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<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXD3</accession>
    <name>A0A9W8XXD3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RPS12</name>
        <name evidence="7" type="ORF">N0V83_010869</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXD3"/>
    <dbReference type="OrthoDB" id="10249311at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000005">
        <property type="entry name" value="40S ribosomal protein S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000530">
        <property type="entry name" value="Ribosomal_eS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047860">
        <property type="entry name" value="Ribosomal_eS12_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11843">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00972">
        <property type="entry name" value="RIBSOMALS12E"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01189">
        <property type="entry name" value="RIBOSOMAL_S12E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="6">
        <location>
            <begin position="607"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="126"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="177"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="471"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="253"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="379"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="533"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4361928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="722" mass="79230" checksum="B5A0B336387EA335" modified="2023-11-08" version="1">MAALASSRQTSAPTRQSTPPSNSHGGALWDLAVPLNNSRTSYEQEPYMSEYVTSAHSPRQPLTSQANGFAKPVRTTTWETQDQYPENSQPYLAPDRLNELHKKPSGDMGASNSEQDSVLEYYKSHANNKRGVAGPAKAKAKKKPTKADPNWAAIEHDENNWIHRDKLKEIEIREMEEAGFRVGRASRSNSRSQSRTRRSRDRTNSETTEFTNGEDRYETRKVVSPIPAEDEEAVEDFNSWSPNSYEEVAPELQPSSPRMNHTGRPSTSRLPLPKTSGIPVPSSVADRNAPLPRSRTGSANWNGDAIATNGARIRSGSVGSQVLLDDMEDGRQTTPPYGHARKFSASGSGSPPKSPQKSPMKAKTPGKPNPTSGARKASAQKTQPKSRNTSAASPPKRPGTSGGSVSRPTTSHRPEGEAPWIATMYKPDPRLPPDQQIIPTHAKRMQQEQWEVEGRVGSMYDKDFRLLNDELPDKRSSQIQPIDLEKAQQNETWPLPSPTKQPPSLEKLDTNVAKSPTTEQGGYKLTPTIPQGPRAPSPKLAPPIAPPQERPSTTRLPEPKEEAKEKQGCCCISDGEDDTKSQVSAAAEVEVSADASAGKGQMSVLEALKGVLKLALIHDGLARGLREASKALDRRQAHMCVLNEACEEEAYKKLVVALCSEHKIPLIKVPDGKQLGEWAGLCQIDREGNPRKVVNCSCVVVKDWGEESQERSILLNYFQTEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXY8</accession>
    <name>A0A9W8XXY8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Defective in cullin neddylation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">DCN1_1</name>
        <name evidence="3" type="ORF">N0V83_010618</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXY8"/>
    <dbReference type="OrthoDB" id="27198at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032182">
        <property type="term" value="F:ubiquitin-like protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.200">
        <property type="entry name" value="Cullin, PONY binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014764">
        <property type="entry name" value="DCN-prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042460">
        <property type="entry name" value="DCN1-like_PONY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005176">
        <property type="entry name" value="PONY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281:SF31">
        <property type="entry name" value="DCN1-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281">
        <property type="entry name" value="RP42 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03556">
        <property type="entry name" value="Cullin_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51229">
        <property type="entry name" value="DCUN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DCUN1" evidence="2">
        <location>
            <begin position="52"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU410713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="271" mass="30409" checksum="436ABF9DF8FA6FB6" modified="2023-11-08" version="1">MRGNDLLKLVPFVADPETKLKLQSVAPENVAKPWARFYYGGGGIGGSTASPASKTTLNKLFDKYREAGAADADIVGAEGTMKYLQDADVDVEGLESLAALEVVQAPTMGEMSREGFVNGWQDSSCDTIDKQKAFIKNLKRELPGNKDLFTRIYKYTFTIAKTSGQKAVALDAAIAYWELLFDSPVSAVRWTSPSTPWLTWWTEFLTSSWKKSVNKDMWNETLKFAQLTLSDEAMSFWNEESSWPSVIDEFVEWVKNEKRGGSKEEVMEEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYR1</accession>
    <name>A0A9W8XYR1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010655</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYR1"/>
    <dbReference type="OrthoDB" id="3798067at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053185">
        <property type="entry name" value="SET_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332:SF2">
        <property type="entry name" value="SET-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="24"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="339" mass="38459" checksum="9DED011FE26C5736" modified="2023-11-08" version="1">MCMVTIILVIDTSERKLSDGTANDRITPANVLGGFGMVATQNIPKGTLILEDNPLLVVDTHILSDNWNGGTAFFNNERASITNRIRYALGSTDWRNKRPRFRLLDDGSPRLVVPNPPNLRVRDVNIVDTNAWGFRNPGVNENTWLVVLGDLSRVNHSCVPNAFTTEVCTNNGQVGRMKLIATKDIEIGEEIVVEYTKEDSWLQPCNARRRTLQRGWGFQCHCNACEDSGFTNCEWRLARMLKAEIDLLPRPTAAESFLLAKRIYAANKYVGILEFWGFGDRRLSEAYLRLAELYVLAEWYAEARKVTITGLEIEGWYYDINEVGRELNQMLARCGVRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ10</accession>
    <name>A0A9W8XZ10_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010896</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ10"/>
    <dbReference type="OrthoDB" id="2962993at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000057">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF38">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="396"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="431"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="463"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="69"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4361955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="496" mass="54413" checksum="00E56C641381AFAD" modified="2023-11-08" version="1">MTSHHPDRDAWKTEVEHIGNLTTTDPKQDALHSETVVAHEDPDLKLVSAPFEEKTEEEKRLVRKIDFYLMPTIWILYCFSYMDRTAIGNAKVAGMARDLGLTSNDYFLAIVVFQIGYVIFEVPSNMVLSRIRPSLWIPILMIFWGTISALLALVKTPAQLIGVRFLLGVAEAGFSPAVLFIISNWYRRHEQSKRFMTFLSAGILSGAFGGIIAGAIIDSLDGAHGVSGWQWLFIVQGVATVGCAIPAPFLLLDWPSTAKQLTPRQREIALARLKADGMHVSASGDGEKSVTHTKALKNAVMNWRLWLLCIGYMTIIGCYSLSYFNPTLVAGLGYTGSEAQYMTVPLYVVAFAIAVPTAIFADRIPPFRPILCAGALFLGGLFCALSAGIYAYIPRYVFLVFINSMIWTANPLALSYASVSMGPLDPETRAISLALINGMGNLAQIYGSYLFPAEDSPKYLKGFGTYAGLLGLGAGIYVAAFFLIKKSPFRARIPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ32</accession>
    <name>A0A9W8XZ32_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009823</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ32"/>
    <dbReference type="OrthoDB" id="5379420at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4363528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="456" mass="50918" checksum="260D1B9431110167" modified="2023-11-08" version="1">MDAHKADSIIADFLHQKNNMDYGSNIQRLAKKYHVDLEDLSSLAIVTFRIPDLSDPKKKAMLPPSEHKTIAGLLLQGCAQAEDPLAIVHILTAVYSSSSSTDLAGREIALLFPQAEITKYRGILEKLGSKAKSIMLGPNVLTLQGLFLERDGKIEKACGFYKEAIICSHLKFQPGGHPMQLPLIDPWNALGFLLKRSKDPSDQAQAKLYFEKGALEADDPLSYYELAAYEPKGSTKWLQYTSKAAASGHRQAAVNLTDFYEEVNSEHSPVLADKKMQKALNWLLGWKRGSAAALAHEWLQASAKLGHKPSMLKLVDYYEARREHERAKEQLRQVLEPPVSANQVEDVFLYPDCFMRLPIVAPSYSTSSAATLPDTLRRFATQVVFSMLANLIPGLLQNWNIFGYMTRAEGPNFEMFSKSNGLHIGQWLLFWIIGELANLLLKRMINPSSNDGTDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZW4</accession>
    <name>A0A9W8XZW4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_010350</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZW4"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000064">
        <property type="entry name" value="MFS allantoate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF97">
        <property type="entry name" value="ALLANTOATE TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G14700)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="289"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="419"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="449"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="60"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4362257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="508" mass="56698" checksum="E56FDCA1EFEDE199" modified="2023-11-08" version="1">MGLEKTPQTHEDEPVKAVPGDSFPVEGNVDNAYKFMQQHAVGPMTPEDDKRILKKIDTHLLPLMIITYTLNFMDKNALSYSANFGLTTDNHLHGTQYSWASGSIFYLAYMVSQPIVARLIVRFPVGRFVSIATILWGAVLMTIAASRSFGSLMAIRAILGCMESCINPAFMVISAQWWKREEQPLRITYWYLGNSIGQVCGGLLGYGIAHITTGALPRWSYFFLIFGAITIIYGSFLFYYLPDSPMNARWLSESDRAMAIERVRGNRTGIETHTWKWSQCRECFMDIQCWILVAMFFLDDIPAGGVASFGSIVVKGFGFSTLNSTLLLVPLGVIQAICILFAGFMTTRFTNIRTWLITGCQIPALIGAVLLYTLPRSNMKGLLGSYYIVQTHGVVGTLTMSLVSSNFAGYTKKVTAGAMMYMAFCVGQVVAPQLFLSDEAPVYSTAFEAAFSCFALCIVLSVVLHFYLVWTNNKRDKLASVDPQAESLESEFLDLTDKQQKQVFRYCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y096</accession>
    <name>A0A9W8Y096_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y096"/>
    <dbReference type="OrthoDB" id="7202371at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="315" mass="35951" checksum="A013A3959608B24F" modified="2023-11-08" version="1">MRYPNRPAMTVADSPKKRKRALSPLPQRKLPIQPGEQKTTDMAPENKTATELFDEALGIANKDNAYDLAHAELLRVEEQDAWDRKAKPKPASESQDGATETRAAIIIKAIREYERKVVFGNLASEAIPGPDTRDMGGQFLTNKDRIDGESKLYEIAKMVPKGALLHLHFNAELHPELLLVQARDMKTMYIRAISPITSQKDLDETEIGFSVLDPDLVEPHVNIFSKDYPGDALNWKTPEWKWKVWMPWKLFRDEFNKQEYTKRYITQEDPTPKKEPTHCGAEPGHVRLLPAENWLKSKMVLSQEEAYGFTQTVNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0L7</accession>
    <name>A0A9W8Y0L7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">nitrilase</fullName>
            <ecNumber evidence="4">3.5.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_009439</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a nitrile + 2 H2O = a carboxylate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21724"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18379"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="EC" id="3.5.5.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbon-nitrogen hydrolase superfamily. Nitrilase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.5.1" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0L7"/>
    <dbReference type="OrthoDB" id="10250282at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000257">
        <property type="term" value="F:nitrilase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044149">
        <property type="entry name" value="Nitrilases_CHs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46044:SF14">
        <property type="entry name" value="ARYLACETONITRILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46044">
        <property type="entry name" value="NITRILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00036406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4363985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="219" mass="24474" checksum="2829C8E8F7F23EE7" modified="2023-11-08" version="1">MRRIQDAVKEAGMFVVLGYSERDGASLYMAQSFISPEGEIVHHRRKIKPTHVERTLWGDGQADSLNLLRYYEYSQRVQIHVASWPAEFEMPDPEKQSWLYHETGEASYRASQFMAIEGQTFVLVASQIITEANLEKNNLVGNHVTKAPGGGFSMIFGPDGKALCDPVGVGKEAVLNADIDLRDIDYAKTFIDTVGHYARPDLLSLMVNPEAAKHVTTMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0M7</accession>
    <name>A0A9W8Y0M7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase S53 activation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0M7"/>
    <dbReference type="OrthoDB" id="409122at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11377">
        <property type="entry name" value="Pro-peptidase_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015366">
        <property type="entry name" value="S53_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050819">
        <property type="entry name" value="Tripeptidyl-peptidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218">
        <property type="entry name" value="PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218:SF19">
        <property type="entry name" value="SERINE PROTEASE AORO, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G10250)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09286">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00944">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S53 activation" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="279" mass="30965" checksum="51ED3ECC8AB8200A" modified="2023-11-08" version="1">MFAPHPEASETTLDWLVASGVDSKRIKHSVGRNWVEFSASVAELEKLLNTEYHYYQHKESSGFRIACDEYGLPEHVRKHVDFVMPTIQLDGLRPVAHARATIEASVNITGLSGTKNCATLITIDCLRAIYNIPVGKYNHSGNQLGIAEWADYLYLPDLKTFFENFTSPKIPSDVVPEFISIDGGKPANLSVAQAEEVVESALDFQTAYSIIWPQQTRLYQNGDSVNVDSVGTFNIFLDALVSNIRSTHCLTTPLTCSGWILLHIPGRRPTIRGSSISRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0P4</accession>
    <name>A0A9W8Y0P4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">Signal recognition particle subunit SRP68</fullName>
            <shortName evidence="10">SRP68</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SRP68</name>
        <name evidence="12" type="ORF">N0V83_008985</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). The SRP complex interacts with the signal sequence in nascent secretory and membrane proteins and directs them to the membrane of the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the SRP68 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0P4"/>
    <dbReference type="OrthoDB" id="10255118at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15481">
        <property type="entry name" value="SRP68-RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3450.40">
        <property type="entry name" value="Signal recognition particle, SRP68 subunit, RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026258">
        <property type="entry name" value="SRP68"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034652">
        <property type="entry name" value="SRP68-RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038253">
        <property type="entry name" value="SRP68_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12860">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 68 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12860:SF0">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16969">
        <property type="entry name" value="SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038995">
        <property type="entry name" value="SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0733">Signal recognition particle</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="366"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="592"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="386"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="596"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR038995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4364392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="622" mass="68275" checksum="5546E8D9984FCAB7" modified="2023-11-08" version="1">MDITKFVVDFRESAFLLGDYGSYRAQLSRRLRIVRRKLGRATPKNAKYAAKAPVTAEEIGKNIEALHLLLLTSERAWAQAMAMKASHSEDNADKNITGSTRSHIVSRLHKAVQNAKQIVDLVGDTAASGATETDVLEAKAYVYALAGAEDFEKQAEGIKGNNATPQRWNSCLTNFSAARIIYSALLKSTKKDLFKDVLAGTTDPSIRYAAYQSRIPRTVGVPAVARKFFPKNDEELLQAVQRLDPAALQEEEAASSHSQITWRGRTANVVDAAIGQALASVDTASSVLDQSPTSSTSSKDRANAYDDILIASQDAVDATRRAIEELEKEGVDEGDARMQDLRVTNLAVNYDLISWRVGRNRVLIGADDGLTFPPNPPQKPRRPRKDGKEWAEREEPTGRKLARLRERIALYDAILQSIDSIKELRGAARDTGFISELDGQRAYFQALKSLNLSHSYAFLSTPKQALALCNRAFSLSSQAASAPRSAAASSSSKAPRLGVSEEQAQSLKQNLENLNSHYRGVVALSQLSDTASKTGTTNAAPVVERLNEYPVSGSVDLKNLVTWPPKLKPVPVKPLFLDVAWNYIEYPGRTQQVKAPEPEKAQVKEKPAERPQAKKGWFSFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0X3</accession>
    <name>A0A9W8Y0X3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small ribosomal subunit protein uS4m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_009107</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0X3"/>
    <dbReference type="OrthoDB" id="3356781at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000025">
        <property type="entry name" value="30S ribosomal subunit S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_uS4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF4">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US4M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 10" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RNA-binding S4" evidence="12">
        <location>
            <begin position="124"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="183"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="195"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="210"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4364512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="423" mass="48165" checksum="D39809EB433F95CD" modified="2023-11-08" version="1">MPRKKPRFHSLKKLNVRQDWSRWSLYSLSQLRAPNVTSRTYFQQKWTAKASTRAYHGEQIREKKWKRQFSRNLPAVVGMDHKYLARYDGSEQAAGRGAGADSLEKSRTPPMTPYMQMAYYPLERRLDTAIFRALFASSVRQARQFVVHGLVKVNGKKMPYPGYMLNPGDMFQVEPSSVMFATGAQKSRSATARRVAKEKATARAEAREQSGGQTPKQPETEVSFTSTREEPTPTELKKHLQDLMEQVDQVLVEDVKAKDKQKFRAFRQSVKKAISLWRSASPETISTLDTQFDFLKTQIAERAAAATSSSSSSKNSAATAAEPLISEEDQARLRSAFEKLRLETEHTSAWNSRNAAAPYATPWRPRDYMSAFAFIPRYLEVNQNICAAVYLRHPVARPGLAEVPTPFHIETGQLAFNWYLRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y193</accession>
    <name>A0A9W8Y193_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acid phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_009658</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y193"/>
    <dbReference type="OrthoDB" id="6509975at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016274">
        <property type="entry name" value="Histidine_acid_Pase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963:SF23">
        <property type="entry name" value="3-PHYTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000894">
        <property type="entry name" value="Acid_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040811455" description="Acid phosphatase" evidence="5">
        <location>
            <begin position="20"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="335"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="51"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="249"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="420"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000894-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000894-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4363366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="480" mass="53580" checksum="C569715A620BB2ED" modified="2023-11-08" version="1">MERIINLLTAALAIASVTADNFNVLQHLGGNGQWFPGPEVTGISSDVPAGCKVDLVAFFSRHGSRYPDTGAYNEWTDLYKRIHASNSFNVTDKKLEFLKTWTPVLSYPDRQIAQLSPTGYKELHEMGATYRLRYPDLYEYNTPFTMWSNYYKSSPRVRDSARLFAQGFLGPNATELGTIYALNSSDSSSWMNSLATSDLCKAYDDEGGSPYKDVWDAIYLPPIRSRINAMIQGDFNFTQSDISIIPYLCGFETQITGRRSPFCDVFTEEEIVQYEYSQDLRYWYGTGLGTDIEKYQMLPIVEMVAKRFVDGPNATYTVGNSTFVPPKIMAAFTNDGQINQIIAALGVFDNEPQLPGNMSLPQRKFRASRLVPMRGTVAFERLTCAASASNTTCGYDNEDNSSQAYMRIRLNDVVYPVVDCTSGPGFSCPLSQYQTILRGKRAEAGSFTKLCNVTDPSFGSEPTATFFMDNTLSFASVIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1G3</accession>
    <name>A0A9W8Y1G3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Monothiol glutaredoxin-5, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">GRX5</name>
        <name evidence="8" type="ORF">N0V83_008834</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1G3"/>
    <dbReference type="OrthoDB" id="415696at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015036">
        <property type="term" value="F:disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03028">
        <property type="entry name" value="GRX_PICOT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000005">
        <property type="entry name" value="Glutaredoxin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033658">
        <property type="entry name" value="GRX_PICOT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00365">
        <property type="entry name" value="Grx4 family monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="GLUTAREDOXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293:SF16">
        <property type="entry name" value="GLUTAREDOXIN-RELATED PROTEIN 5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="7">
        <location>
            <begin position="54"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4365216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="154" mass="16976" checksum="F583BA878084FA28" modified="2023-11-08" version="1">MFSRRAIPSAFRAATRASTARPLPAARFLTPFQARLLSNEVRSAIDKAVASAPVVLFMKGTPETPQCGFSRASIQILGMQGVDPEKFTAFNVLEDQDLRQGIKEYSEWPTIPQLYVDKEFIGGCDILMTMHQDGSLAKMLEEKGVVVPAEAEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1K1</accession>
    <name>A0A9W8Y1K1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_009514</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1K1"/>
    <dbReference type="OrthoDB" id="47007at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48107">
        <property type="entry name" value="NADPH-DEPENDENT ALDEHYDE REDUCTASE-LIKE PROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48107:SF7">
        <property type="entry name" value="RE15974P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4364059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="28270" checksum="A3430D5879E431DD" modified="2023-11-08" version="1">MALTYPLANKTALITGASKGIGAATALELSRLGANVVINYASDAAPAEALVAKIGSDKAIAIKANAGDVKEIERLVQETVAWSGSGGKIDILIPNAGVSIMSTVEQTSEEDFQKTYDLNVKGPYFLVQSLPISLESWEEDVGKGKALPYLQKGSHVVMLSTSLSVVSSITPNYLLYVSSKGAIEQMTRVLAKDLGQKGICVNAVAPGPTATDLFFKGKPQALVDTIASWNPFKRIGEPAEIAKAIAYLSGDGGSWVNGQILRVNGGMTVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1M0</accession>
    <name>A0A9W8Y1M0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_009544</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1M0"/>
    <dbReference type="OrthoDB" id="3233595at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08249">
        <property type="entry name" value="enoyl_reductase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047122">
        <property type="entry name" value="Trans-enoyl_RdTase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348">
        <property type="entry name" value="HYPOTHETICAL OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348:SF5">
        <property type="entry name" value="OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G01420)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="4">
        <location>
            <begin position="9"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4364089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="337" mass="36485" checksum="5BC6BB4571F047E8" modified="2023-11-08" version="1">MKEAQISKGLKTVIKDVPIPEPGPDQLVIQVIVSGSNPKDWKYPELIANYSGNSGDDIAGIVHSVGSNVWEFKPGDRVASFHEMITPGGSFAEYAVGWQHTTFHLPKNVSFEEASTIPLAAMTSAIGLHLRLGLPDPWTPAKESIPLVVYGGAGAVGAYAIKLAVLANIHPIIAVAGKGEKFVESIIDRSKGDTIVDYRKGDEAVVQGIKDALKGAKLQYAYDAVSEHNSYTNIVKVLEPNGHLTLVLPGKKYEGVPESVNMTITMVGTAHKEDKDFAYVYFRYIARGMAEGWFKAHPFEVVPGGLEGVQRGLQNLKDGKASGVKYVFRIGEVKEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1M7</accession>
    <name>A0A9W8Y1M7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GS catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_008196</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glutamine synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1M7"/>
    <dbReference type="OrthoDB" id="3364440at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000074">
        <property type="entry name" value="Extracellular developmental signal biosynthesis protein FluG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43383:SF2">
        <property type="entry name" value="AMIDOHYDROLASE 2 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43383">
        <property type="entry name" value="NODULIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS catalytic" evidence="4">
        <location>
            <begin position="605"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="465"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="851"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="858"/>
            <end position="874"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4365576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="950" mass="107219" checksum="7200A3D209CE35ED" modified="2023-11-08" version="1">MESNTPLLRPSIDQLRHVANNYPIIDNHAHNLILPKHSSTIPFESITTEAQGRALRDTFKALPHLRAARQLRQLYECGEDADWEDILEQRVEWLRSDPERIAHRCFEGVHALLIDDGLAGSEKVYPYGYHDRYTKAPSKRIVRIETVAERLMEDIIRDATEDDLGKTKFLPDTWLAFTDDFEREIKDAMKDPDVAGFKSVICYRTGLDIEPDYEHAARAVGHPFERYVKSCIRKREYRVEKKALNDYLVLRTLEILSERLPHSDSLAKPLQLHTGLGDNDINLLESNPAYLQPLIEAYPNVPFVLLHSAYPYTREAGYLATVYRHVYLDVGEVFPMVSRDGQTAILRQAMELVPGSKLLYSSDGHWFPETYWLANMQFREVWLEILIEYIDKGDLTPFQAVAMTKDILFNNSNILYDLRYEAVFDETIEEAPKQLTYNPKTDVGTPHLSPPVYPAQNAGLASRAAGFDTSPARSPSPFSPSFPPPPKVPQVYDTQLFDKFTQENPDVKFIYVQWLDYMATVRARIVPIKEFSRMIREGSRIGISQGNTGTLQNDALTPIVNTTGQIYVEPDLRSLRRTHNKDPLPSATVLSYWRDENGNAIRECPRNNLEALINSLQYNHSTTLLIGFEIEVTFLSRNASSSSNTTANQPYCPLTKTHAWGTLTPEQWLQLPFLSEIVLALETMGIEIQQFHAESGQGQYEFILPPNPPLLAIDTLIQARQVIAQIAALHGLRATLHPQPFEGIGTAAHAHVSLHPPDREMPFFVGGVLRHLPALCAFSMPEAVSYGRVVDDHWTGGTYVAWGTQNRETPLRRVSKGRWEVRCLDGLANMYFVVAGVIAAGLLGLAGSPLPMPSSTPEEEHGGGGQQFEEKDVDVNPSTLDEQGRARYGITRKMPRSIEEAIAALKSDGELNEALAHGLVQDYLAMKKSELDMLEGMSEAERRVFLIERY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2A6</accession>
    <name>A0A9W8Y2A6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein cwh43</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CWH43</name>
        <name evidence="5" type="ORF">N0V83_008543</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2A6"/>
    <dbReference type="OrthoDB" id="68581at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000031">
        <property type="entry name" value="Calcofluor white hypersensitive protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057315">
        <property type="entry name" value="Exo_endo_phos_PGAP2IP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051916">
        <property type="entry name" value="GPI-anchor_lipid_remodeler"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053912">
        <property type="entry name" value="PGAP2IP_TM_1nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053911">
        <property type="entry name" value="PGAP2IP_TM_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14859">
        <property type="entry name" value="CALCOFLUOR WHITE HYPERSENSITIVE PROTEIN PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14859:SF1">
        <property type="entry name" value="PGAP2-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23022">
        <property type="entry name" value="6TM_1st_PGAP2IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23021">
        <property type="entry name" value="6TM_2nd_PGAP2IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23226">
        <property type="entry name" value="Exo_endo_phos_PGAP2IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="251"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="303"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="647"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="PGAP2IP first transmembrane" evidence="3">
        <location>
            <begin position="23"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="PGAP2IP second transmembrane" evidence="2">
        <location>
            <begin position="181"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="PGAP2IP C-terminal nuclease-like" evidence="4">
        <location>
            <begin position="413"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4365921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="697" mass="77531" checksum="BE7D7FD4839E86F8" modified="2023-11-08" version="1">MERMRMVYVPAFCFLKHTDCLDFPLWYMGISGYEALVMCTITPFFLGIRPLRFLVSRYVWFFHLLSLSGLLAFLAKTPVNRLFAVGFGLSMACLSWAATFYAERSQPHRLEARISAFSLGLIASSVAKFAFWTNNPIWPIMHEPNGGWNKTGLVLAVIAILISTRSTASSGADIPAPGPTKGSPVLASFGLAGLFFAMHSLLSDSSTMISWVWEGYPVRGPLAVPHGTWTLLAMGLGLNIGLFAPNISRSWAFYGLGSIGAAVLTKYSHWSGYYGAFVLAVYTMAAAPVLITHAARHSPGKTFGLAFLVYNFMVLFHVWVVAYAFVPGGPLVRERTDWVMTAMMLQIGAGVFSVSAQPPASKTYKGKAVITATASRQRSYYLYILGALELLAIATAYLRFPSYDYTPYHPETKSITAGIWTIHFSLDNDMWSSEHRMRDLIRELEIDVIGLLESDNQRIIMGNRDSTQFLAEDLGMYADYGPGPNKHTWGSALLSKFPIVNSTHHLLPSPVGELAPAIEATINAHGHLVDVFVFHSGQEEDPEDRRLQSEFLAQRMRATPRPAILLSYLVVKPGEGNYKTYVGEKSGMRDIDGSDWDRWCEYILFKGLRRAAYARVSRHTITDTELQVRSSLFFFIIPLFPLPPSHLLVSPCSLSFFLLSFFFLASHRIAREECANVHVHVNRSVNSSSASRRTATN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2B7</accession>
    <name>A0A9W8Y2B7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008558</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2B7"/>
    <dbReference type="OrthoDB" id="5290825at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="183" mass="20222" checksum="9BDB1191E5AF5EBE" modified="2023-11-08" version="1">MDGKEVRPVEDEKTAMDKEHATHNEKAALGNQPELEHAENTGRRQSVALNIIENPLRRVTAAQAALDARTFAESNGMEQHADVFGRAALVARDPERFEMVNELTEDERAALIYERDHKWNGPVMLWYSISLCAIGAATQGWDQTGSNGANLSFPEEFGIDGTGRDEWIVGIINAIIFLTAGLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3S4</accession>
    <name>A0A9W8Y3S4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Polysaccharide synthase Cps1p</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_008003</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3S4"/>
    <dbReference type="OrthoDB" id="3828420at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06434">
        <property type="entry name" value="GT2_HAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052427">
        <property type="entry name" value="Glycosyltrans_GT2/GT47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47844:SF1">
        <property type="entry name" value="EXOSTOSIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47844">
        <property type="entry name" value="SYNTHASE CPS1, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G02500)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13641">
        <property type="entry name" value="Glyco_tranf_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="443"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4366367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="465" mass="54058" checksum="45411742F468A219" modified="2023-11-08" version="1">MFPFSLALFPNWSWFCIFLALFLFRYVRLWVNLASNWTYTPIQPVDDPTITSKDMTVIIPTVAEDLQQLKETVQSAYRLEPHELLLVTPDSRVKRVYQMVEELGTPKLIQVLSVSQANKRRQLCRAIPEVETKITLLLDDDVWLPEKFTKWILAPFEDSRVGGVGTNQQLRRSNRSNIWEFLGAIYLVRRNFDCTACNWIDGGLPCLSGRAVAYRSEILQDPNFTYGFTHETWGSDHFLNADDDNFITRWLFSHNWKIQLQNHRECEVETTLEDDSKYLRQCLRWVRSNWRSNLTSLSEGHMWMSYPWSLYAVFQTTLTQWAFLLDCLLFASVYFALSEAGFYEAEDDRQGRAEQWQRSLLWALFIAHWVFAKTIKLVPHLLRNPGDVRFIPVSILFGYFHNLIKLYGCITVTETTWGTREGADADDNIRMLPIPPMATITPPLTPPPGGRMLRERGTLFPSSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3T4</accession>
    <name>A0A9W8Y3T4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007666</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3T4"/>
    <dbReference type="OrthoDB" id="3670233at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="511"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="553"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="553"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="762" mass="82822" checksum="9EBC64EED7E258E0" modified="2023-11-08" version="1">MASTNINTNTTSMEQKIADMEEKIANVEQKVADVVTGTNHTESATKEDIKQLKVDMAQMIQAMLQQLLGAGATKAILQPDPATESIVSIPTQAVLDRSDNVLEASTSTYNHGIHVLPQSVNPNNIDISNATTSGGTAPSLPPTEDMFPILRTAIEVADSDVLVAHVASPQSHLPLPVDNAVEPRRVRDLSRVQKQLSGSTRQHNIKISEMATRSLSCAIEHFVAVSAKTDPQATVEHATQELQSKLGLTATARSIEVLQTITQNWSLKAETVHQKLVAASQISAASDEEKLSSEDLDKLRPIPLVSGPLLCISQNERYDTTIEIYRILKAMNPDPDLHSTCRLADNEAFKLAQHEEICIFGGLHAKLIKGRTLPRAAIVDEYHARKSSRCKQYMYRTGFKKGVKAFHFFVRDALKNARKKVQNRVAQSVHSARNGMHRVAGLLSGPPPPAVTVRSVEIDTDIVDTSGHETQVTSPATEQVVSGECENVPATTLPVAQIDLSQAFTSMALQSSAADDTQPTTVASSTDGQRASGGDEEIPAMASLVAQIDPLQTSTSSALHSSAVDDTQTNRHHTVPATPAPSANQDASQSPSTVPQPQTPGIPTSDQLRDKDLAQLRKTSSPTPDMLLISVPTRSDTIVRFYNTFVEANNTIVKLSKGSEVDLLGELMEAGSAPFWDNFVSLSADNLMDMAKKFEADIVKKLRRKVRKREVRNRGHKEEYVKMAEKIDVSLDADGIEWFAGQAHNWRELELDGLGLEGLGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4G0</accession>
    <name>A0A9W8Y4G0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MFS transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_008112</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4G0"/>
    <dbReference type="OrthoDB" id="5086884at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050930">
        <property type="entry name" value="MFS_Vesicular_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506:SF23">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040976408" description="MFS transporter" evidence="7">
        <location>
            <begin position="24"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4366476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="20404" checksum="83D02E40F872FB92" modified="2023-11-08" version="1">MAYGLIGLDIVLRLLLVEKKVAARWDPDSVGRVPVTAGEEFGDDMAVSPQPQDQEKAIPAAPDQLPLALRFVDENTIKDKVLLCALLALIGLFLSLTFPPIMAEISSIVEAKEKSMLAAGHPGFGPGGAFAQAYALFNVAFAGGCMVGPLLAGFIVQDKGWATMAWVLGLLSGITSLPAVLWLGGWLLQKTSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4X5</accession>
    <name>A0A9W8Y4X5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007124</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4X5"/>
    <dbReference type="OrthoDB" id="2430277at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH2" evidence="2">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="198"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="424" mass="42302" checksum="A563618D240DAC29" modified="2023-11-08" version="1">MPPMGRGAGGPPPPPPMPGMKAPGAKPPSGAGRGALLSDIGKGAKLKKVTVVNDRSAPIVGKGSDGPSAPPIGGAPPIPGMGRPPAPPGGLAPPVPAGGNRARSNSDTGGGDSAMASAPQLGGLFAGGMPKLRKAGGVNTGADQSNTPYLSDPETSRSSVPKPPRAAAPKPPGAPAPPIPGGRPPLNPVFGGLRDSRPSSLRTSTSSIHDFSAANTSSSTSTTTFVSPASPSTIDTASPPSAPPPPNAQADDDDDYDPYRYDSRAPSAPAPLPPKAPTNGHTPSLAEQAARNAFGRASPVAPPPPPPPGSAPTPPSSVPPSAPAPPPPPSAAPSRPTSQTPLRSMLDPSSYTLSNGGSGIKSPTSPGMATGGGGHKGKIIPIHDLRWRFQDEGQFPRPREFTGGMKRYRAGRGSSVPLDFSAYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5E3</accession>
    <name>A0A9W8Y5E3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5E3"/>
    <dbReference type="OrthoDB" id="5426165at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40623">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40623:SF2">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="399" mass="44182" checksum="82049F329679083C" modified="2023-11-08" version="1">MGFGTSPGHEFFNSWALWQKMTFLRKYSKVDKGKQAQTPEMLEAQPVTAVSTETKDEIPFGIRAIQSGIEIDGVWISRSNTPVGSSRASIISEHKPMRSYNNSQLELPQAVVHGSSRNSSRAPSSFDRAVSAERIPTNDSRSSSPGRGPNSAPRCARNATHGETGSSSKSSRRTSDESDYMAITQDVRAYESAYIRPASLQAPIDPRTDLDLLQSHRLSHVAETGQLTPRVRRPGNSGEWASVAEHQISSLNGVNYFVPQKTPSPPLPPVIDSQEEIGVASSFMPTQDGHAVNEARQAVPLTESYAPKAAYYPDAYQPRGPHHQYSYDEVPYEVNTDQNQRDSQVIRKVNSGFEILRPGTFQPPTPEELELAERRQSKKLQKKRRSSGDSRQSAFVEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y658</accession>
    <name>A0A9W8Y658_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006506</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y658"/>
    <dbReference type="OrthoDB" id="10261522at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044715">
        <property type="term" value="F:8-oxo-dGDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03676">
        <property type="entry name" value="NUDIX_Tnr3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000019">
        <property type="entry name" value="Thiamin pyrophosphokinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031804">
        <property type="entry name" value="DUF4743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622:SF8">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15916">
        <property type="entry name" value="DUF4743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="132"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="314" mass="35529" checksum="2727371831476251" modified="2023-11-08" version="1">MSHSNLDLVNQVDGFPYPDSNTDQYLATINTYYQLRIAGHDYALGYMLPSVAEVFRGIPNWQLDDDDRALTLTGGSNAEERSKAIETTLLAMRETGHFKVLNKWRDELYAVYGKGKEVLFNVERSASPLFGVVTYGVHCTAYTKKDGQVKIWTPRRARTKQTYGGMLDNAVAGGIASGESPFESLVRECAEEASLAEELVRSRAVPGGTVTYWYIRDERAGGETNLMQPECQYIYDIELPEDVIPKPGDDEVEEFYLWSVEEVQEAMKKGEFKPNCALVVLDFLVRHGILTSENEKDYIEIVSRLHRRLEFPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6H2</accession>
    <name>A0A9W8Y6H2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_007491</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6H2"/>
    <dbReference type="OrthoDB" id="1933717at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43391:SF14">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY PROTEIN 7-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43391">
        <property type="entry name" value="RETINOL DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4366961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="346" mass="37103" checksum="820C9EF78FC921DB" modified="2023-11-08" version="1">MSHHRPKDTTLASTFTPTTHAKATPPRPVRKPLPKPLNVLIIGASRGIGEHIAYAYAHALSTHPSSHLILAARESSSAELAAVAQRARACARGQDGVKPSSSTTTTGTTMTITVTTLSVDITSSPSVACLAASVKDLLTTTSQRGGLDVVVLNSGYSGPVVLKVHEGNPQDFQDVFDVNVQGTYLVAHYFIPLLRDHPGGSSAARTFIVVNSFAACIINGHIANTAYCVSKFAQARLVEFLAEQYGGGDGEQEQEGVLAIAVHPGAVNTEMADKTTPESFRPYLTDDVGLCGAFCVWLSRERRMWLSGRLLGATWDVDEFLAKKGKIEEQDLLKFSYRVGDASNRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6M9</accession>
    <name>A0A9W8Y6M9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_006810</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6M9"/>
    <dbReference type="OrthoDB" id="4161376at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010573">
        <property type="entry name" value="MFS_Str1/Tri12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF109">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06609">
        <property type="entry name" value="TRI12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="390"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="447"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="558"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="59"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="590" mass="63909" checksum="5BE2D55EC4E8D163" modified="2023-11-08" version="1">MQETSPKFTQASHDVEKDEVQHLERVTSDAAPSEIEKRQLAPVVSRGPEIHEKMNARLFMTLLCMSFLWIGSQIPLYLFGSVLPIIYQDIGGFDRYVWFVIGYLIPNAALCPFVGALSDLFGRQKVAIVGQVALIVGPIITATAHTMNIAIAGNVFSGIGAGLNELIALAGTAEVVPIKDRGKYVGLVVFTIAPFCPSVLYAQLIAHSSNWRYNGLFVGVWNFVGLLLCIFCYKDPSRLTEEYTARHVLRQVDYIGGILSTTGITLFMMGLQWGASQYPWGSAHNVAPLVLGLVLIIAFFIWEFYAPYPMVPRALFSKAKKTMIVILLITFLSGGNYFVMLLFWPTQVYNVYGNDPVGIGIRSLPIGFGIIGGAVICLVLIPLTKGRIRALMIFFTALMTAGTGAVSISNPHNLHTVLAIVSLASIGVGGVIIPSSIIAQIACPDDLIATITAITLSIRYIGGAIGFAVYSNLYFRKVTESLTEILAIETLAMKAVVNPLTPAGLAIITEITGLIGAAQFDAVKEILATNPLVLNRNAYPMIIASAQEAFSLAYRWPYWVSIAFGAVCFVLSFFVGDITALLTAQVAHPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y775</accession>
    <name>A0A9W8Y775_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005396</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y775"/>
    <dbReference type="OrthoDB" id="5226996at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="417" mass="45179" checksum="E7995917DAC05292" modified="2023-11-08" version="1">MSEDTETSAPPRASRFKEHTNTNGSIRPPPDELWKDLGIEQLIEQFNEENQRPPVRRNPSGTSATSVTTKGAAAPIALRASSETRAPGIYENSSLGNATPVFGVPSVAATGGLAAAPPATPATHEGTFGRFQRAFASVFGNVLGKRKAGSVDAEKDKEKKVLDERKRAADAAYQEAKELGLLPTPKVFVRPTSAVKSHKCVADTATPVRPPRTPTLYRTPSKKDLHKQKKLLRRVSNLESKLASAKKELQTVLHNDDLPPVPPLPTLLPPTPATSQSDLEAPLPDIPTTPEPRSTGKITKKRKATTDDDDDTEYKPIPTDSDGDISMSAASASEPERTVKRVKSSASRKNLKRQTTRLQKRMSRSSISKEEAAVIVVPDGVTVPPIPKMPKSMEKEGKKAAVKRRDDGYGGLEHEMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7E5</accession>
    <name>A0A9W8Y7E5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EDC4-like protein pdc1 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_005502</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat EDC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="21128at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000817">
        <property type="entry name" value="WGS project CABT00000000 data, contig 2.15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055393">
        <property type="entry name" value="Beta-prop_EDC4L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045152">
        <property type="entry name" value="EDC4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15598">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15598:SF5">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24106">
        <property type="entry name" value="Beta-prop_EDC4L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="EDC4-like protein pdc1 beta-propeller" evidence="7">
        <location>
            <begin position="455"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="9"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="558"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="907"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1284"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="65"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="166"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="210"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="232"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="290"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="568"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="907"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="927"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="993"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1291"/>
            <end position="1300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4369739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1455" mass="157640" checksum="758289392032C1B6" modified="2023-11-08" version="1">MTDLQELFARLNGGASANQNQHGHQQPSVSSPIFSPSPGGPQPHHHSAIMSPNTSVPNTPAPEGSVSQQTPQQQSANLLNLLRFNSQPGVGQVRRVSQNAPTSEGPPPHGRTVSATDLVASFMGNRTPSQGPSSAAPASAQPVPSSVPSENPQDLLLRLLNHPKPAQSDGRSSRAASAAFSPPVTSNKPETVVDDLAQDLADAELEKVSSDPAAGAAATSPSPMRVFGSDGSGPSSRFQPAATTLPPKFTYVNPFDQLEAASPRNRTPVPESTKSPAPKVEILKPKHTFEASSAAATSSKEPTAAQPHETVAEAVSDLGEQVGQQIEEALAEAEHKSTAQMAEDLDTLPAEDIAEVQQAVIDIATEIKEEFADTGKAKEIQAALPKPLAAALKEVATEVAEGNVAEDWETAEAAEVAKGEDQTVIVYRFPMRAFASIAVNQMPPRRLYFRPELFMDVARLKKDFDQIDRSLVSASKNFIVYALVKKGGFRIIRQENGNYRQVFENHQERIFNIAVCTAGDEYSTKTLESILGIGVNGTVFWTSLDSVREDQFGEHLDSQGLMLPPSPSQDDNTSGGQLKTRAKPSSRHPEFFAVGRGKSIHIVFPKVAAAYVRHKSRICHTEKYLKERSLKILTGKAGKDFTFSEDDTIIVSLDKAGRMRFWDIRTLTEPELGEPHNAPRQVEVSDTLLEFHTTTPNAKSWPTSVFFFDKDKPYTKGIALRYVMVGMKQNHAFQLWDLGLGKAVQEIHLPHENESDAICSVSFHPRTGIMAVAHPTRNSVFFVHVSCPRYNLPAGLSQATYIARLANKGQPPLPAVTATAIMTSITEYSFASKGQIRSLHMLNEPAGPADVDDPDNAALFELYVMHSKGVCVLRIRRSDLGWKKEGEPIHPKEAEEEGVITITQLKPPQAVTDESSTAGDTPAPKSVSDRSARETLKKENSNVSRQSMTPEAAMRASTLAKVESKQDAARAAFINGAGEKSEKKKKKRSNAETASQVSASVAASTRAPPSYAQAAQATPQSKSPVPPEPPVQQDPTSEDNEAPEWAKKLISKHFQQGTPSAPAAPTAPSAVAEIDAKKLEEIVQGEITKGFSRELDVMYKRFDEDKRVMNAANGAKQEAILRLVSSTLTENVEGVIRKMVDENIRNVLLPEVLAKTSSTLEQSISSNLKSAMGPQLSKEIPDAVSRALKSPQIFQSLSDQVAKKLTGTFEPMVVASVGSVINPAISGLSGLIEKQIGGQLRQAHAQRHEDAAKIAQLTDTVHTCLETIQSMVATQAELQSQVAKLQQSIAQPPPEQPPHYSPKAPVKQKSPEELETEAITRLMADGNYEQGTMQWLQSSRTSELFDNVFVRCNPAYLSQVSPLLALSTGAVVSDALERNLPERLVWLDAVLRSVNPNDAEIRDIVPKIMDVVCQRLTTAYIQLNESTPGSPLLRRISGLVNKCTEMTRAAYGPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7X5</accession>
    <name>A0A9W8Y7X5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006391</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7X5"/>
    <dbReference type="OrthoDB" id="3686891at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="592"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="389"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="463"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="594"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="616" mass="68325" checksum="8607B758E3B4E0F3" modified="2023-11-08" version="1">MKQFPLQEEGSMRQNESHGDDTHLHRHFPPSDLDDTFITYFEDSIHNPQDLDALLEYNDQSNQFYVDANDCSQSHISNMCLAAERTTMHGSQPTEETPPFAVSNDNGLRHATGNYAGLSSSGSRSREAEEREVLISQEGTPHPYNMHRKTVSLASPAMAPMQLLPYPSSRHSSAPPQAVGHSSFAPFGRLFNTSADAKRHRHVKLRFDRHAYDENDDSIALVEANRQHHVERIYNAMTRGDLARDNAGSTAMKRWTREPHYPSDLVEAYAHKVFDCLLGQAKEGFRGWHHNDYVADDRKGEDEDRDVNCAGRLENVITALELEKTICEDVMNSACQIRMFVNAPKAYSNRKHQNRVGNSKRPGAKAGTLDPSNRSSKVRKTGDRRSRARSSTTSELTASTNAAVQPLHQTSTLVPYTNAESQRISSSAPSGSFLAPQAPSMHRAPVRSQRSSFGQHQAIAMAPSTSSSRPPSHTMQLPHIPRITSMPPTQQPPFLSPPPLSHGPFSAPATPDDAKSRNIDPRLIPWQQTATFGESSSSNFPAHPTAGGSPFAQVADWSRNVSTFTPFTPGELAGNTNYFEHHPEIGVNLADVEQQPSNQVEDANQDSESSFFWQFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8Q6</accession>
    <name>A0A9W8Y8Q6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DUF159-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_004321</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SOS response-associated peptidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8Q6"/>
    <dbReference type="OrthoDB" id="2111841at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106300">
        <property type="term" value="P:protein-DNA covalent cross-linking repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1680.10">
        <property type="entry name" value="SOS response associated peptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003738">
        <property type="entry name" value="SRAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036590">
        <property type="entry name" value="SRAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604:SF0">
        <property type="entry name" value="ABASIC SITE PROCESSING PROTEIN HMCES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604">
        <property type="entry name" value="DC12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02586">
        <property type="entry name" value="SRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143081">
        <property type="entry name" value="BB1717-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0190">Covalent protein-DNA linkage</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="302"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="302"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="332"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="382"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4371105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="411" mass="45751" checksum="805E2662A9B62DBE" modified="2023-11-08" version="1">MCGRYALALRPSEVRQQFEHSHMPVEDAPDDDDVRQSYNFAPGYHGLVYRADGPDYGGATGETKYKLQSMQWGLVPFWTKRNPDYGSKMKTINCRDDSLIEDRGMWTTMKKKKRCIIVAQGFYEWLKKNNGKEKIPHFTKRKDGQLMCFAGLWDCVQFEDSSEKLFTYSIITTDSNKQLNFLHDRMPVILENGSDAIRTWLDPNRTEWSKDLQSLLQPYGGELECYPVSKDVGKVGNNSPSFLVPINSAANKNNIANFFGNQKNAAKSETDQKVIAKAEHDLDESTNQNGDVTVEHDVNETRATTDRVEGTENKAPLPVPATTEPQLGDGPKGIKREHSEEDVKGSTADVEPQPKRSKPAATPSPAKSPAKKMSTPAKKQGTRSATSNGSAAKTSAKADGSRKITSFFSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9B7</accession>
    <name>A0A9W8Y9B7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Golgi to ER traffic- protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">GET3</name>
        <name evidence="11" type="ORF">N0V83_004637</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the arsA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9B7"/>
    <dbReference type="OrthoDB" id="1770at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02035">
        <property type="entry name" value="ArsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000235">
        <property type="entry name" value="ATPase ASNA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03112">
        <property type="entry name" value="Asna1_Get3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="ArsA/GET3_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027542">
        <property type="entry name" value="ATPase_ArsA/GET3_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="ARSENICAL PUMP-DRIVING ATPASE ARSENITE-TRANSLOCATING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803:SF3">
        <property type="entry name" value="ATPASE GET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ArsA/GET3 Anion-transporting ATPase-like" evidence="10">
        <location>
            <begin position="26"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="33"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4371420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="340" mass="37734" checksum="33CDD93E53572C3E" modified="2023-11-08" version="1">MASTALIDADAMEPTLQNILDQKTLRWIFVGGKGGVGKTTTSCSLAIQLAKHRKSVLLISTDPAHNLSDAFNQKFGKDARLINGFDNLSAMEIDPNGSIQDLLASGAEEGQDPMAGLGGMGSMMQDLAFSIPGVDEAMSFAEVLKQVKSMSYEVIIFDTAPTGHTLRFLQFPTVMEKALSKVSQLSRQFGPMLNSFLGASGRLPSGQNMDELIEKMEALQKTIGEVNGQFKDADLTTFVCVCIPEFLSLYETERMIQELNSYEIDTHAIVVNQLLFPKKDNPCEQCNARRKMQKKYLDQIEELYDEFNVVKMPLLVEEVRGKEKLENFSNMLVQPFIPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9M2</accession>
    <name>A0A9W8Y9M2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">acetyl-CoA C-acetyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">N0V83_004701</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.9" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9M2"/>
    <dbReference type="OrthoDB" id="5404651at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000007">
        <property type="entry name" value="acetyl-CoA acetyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919:SF156">
        <property type="entry name" value="ACETYL-COA ACETYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="15">
        <location>
            <begin position="30"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="16">
        <location>
            <begin position="298"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="12">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="376"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4371484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="423" mass="44728" checksum="0AEB5E40516D3FD8" modified="2023-11-08" version="1">MASRPQQRLASIQRQFSSSAPQKKEIRDAYIISASRTPTAVFNGNFTTVSATTLGATAIKSALEKAKVPVSSIDAVYMGNVLSGGVGQAPARQAAIFAGLPTNIEATTINKVCASGLKAVNIAASTIELGQAEAQVAGGMENMTRVPYYLNRASQQPPFGHQKLQDGLIADGLWDVYNQIHMGNCAENTAKKYQVSRKDQDDYAIETYKRAQQAYKDGLFKEEIVPVTIKSKKGEVVITEDEQYNKLKLEKVPTLKPAFDKGPDASVTAANSSPLSDGASALVLGSKEIAQQYGKDSRVLAKIITYADAAVDPIDFPIAPASCVEKLLKQSGLSKEDISIWEFNEAFAAVIKANAKILGLGTDNVNPRGGAIALGHALGSSGSRILVTLLHQLEVGQYGAAAICNGGGAASGIIVQRVSADQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9W0</accession>
    <name>A0A9W8Y9W0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005516</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9W0"/>
    <dbReference type="OrthoDB" id="3779826at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="304" mass="32621" checksum="391EEDB54C992836" modified="2023-11-08" version="1">MSRPEAAAEGVASKSTWEQAAAFLTVIDGDLPLFKDADEGRDPDQELYVPRALTAPSASAAAQLSSVSGFHIFHNTAFDHHKEDRKQGQLDNAVDTPLPTSPEAPGVVVSTSQDDDHEHEHVSMPSSDKSSLGFNEQDDDPESALDFMDTQFSKWSHNQREGTDAVIGDVMRASSDIHATLVGLDKKTWTKTPDVLSQATNVDEPEDTAGAADLHDLRHHGSKGKMSTDAAPVEDIISTKDGSVKEQSLLQEPHASKRAAMIAKSKAAGDKSILTGQSANQKRAALQKSKNRKLVDSDSGNLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9Z6</accession>
    <name>A0A9W8Y9Z6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial 2-oxoglutarate/malate carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_004872</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9Z6"/>
    <dbReference type="OrthoDB" id="756301at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000009">
        <property type="entry name" value="Mitochondrial 2-oxoglutarate/malate carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="73"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="172"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4371652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="27243" checksum="3011DF5FA3595F07" modified="2023-11-08" version="1">MVKLIGEGAKGGPRPSPISAAREIIAQGGILSLYSGLSAGYLRQIVYGTARLGLFDTFIDMFEKRAKSNNTKIGFGERTVAGMGAGSLGALIGNPTEVVLIRMQCDGLKPKEQRSNYRSVFDALARITRTEGIRTLWSGAFPTVIRATATNFGQLTFFSESKNQLKRHTSFSAQAATVTASTIAGFFAAFFSLPFDMVKTRLQRQNKAPDGSVAYKNMFDCFVKVAQDEGPLRFYRGFGPYFFRMAPHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAC9</accession>
    <name>A0A9W8YAC9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PLC-like phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_005265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAC9"/>
    <dbReference type="OrthoDB" id="7984201at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF80">
        <property type="entry name" value="PLC-LIKE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26146">
        <property type="entry name" value="PI-PLC_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4370744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="294" mass="32702" checksum="D006731DEFCE215D" modified="2023-11-08" version="1">MRVTSPCILEPRNESARVLCHSSCALFDVGPLYKWLWEIRVWLDAHPDEVVTLVLVNIDFVDARELEAEYSKADIAHYGYVPPVIDKAPPPSTKYNSTWPTLGEMIDKGERLVTFVNALVPDKDNAPYLLNEFDFIWENQYDITSLDDFACTPDRPSNTTSIAEEHTSGKLFMMNHLLYWQQAFGIQIPDTRKITATNSWDGPGGLGTHMIQCGSQVGRQPTFVLVDFFNVGPAIDAVDIFNGVRQPVGRLNVTTEVTDGTTMMRKVNGGGRSVRAPVLAMVVIALGTILRTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAJ5</accession>
    <name>A0A9W8YAJ5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AMP deaminase</fullName>
            <ecNumber evidence="4">3.5.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Myoadenylate deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">AMD1</name>
        <name evidence="12" type="ORF">N0V83_003893</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.6" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAJ5"/>
    <dbReference type="OrthoDB" id="1723809at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003876">
        <property type="term" value="F:AMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046033">
        <property type="term" value="P:AMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01319">
        <property type="entry name" value="AMPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.20:FF:000001">
        <property type="entry name" value="AMP deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000214">
        <property type="entry name" value="AMP deaminase Amd1, putative (AFU_orthologue AFUA_8G02860)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000300">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006650">
        <property type="entry name" value="A/AMP_deam_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006329">
        <property type="entry name" value="AMPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01429">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359:SF0">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19326">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00485">
        <property type="entry name" value="A_DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="285"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00078830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4372120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="887" mass="100509" checksum="A39CEF2AD74277C6" modified="2023-11-08" version="1">MLKGAFSADADGSPDMGPVHEDQMPSSEEDELSPDYSEPEAGRGRRARTDHGATATAQDTAGTGVDDEDAAGMLPRDKQRRTAFYDYTAEKQMSHTEAKQFYQRHQLETQYGGSQAGDAYSPAMRAKTFPPNFGGTDGSDFPGRAESIRSRKSNVSLANQGHRFTLPIGVPHTEQSQDPRPHMTPNAANHQGSYGDADALLQADQLARSHAQHPALPREPKPLLAEEGIHGAGAGIGVGSGAGGFAMSDSSVTAELSAIYTNVQKVLDLRHKYIRLSLQRNFDNPKDDPGWRIYPPHPEPVWNDSSKERRNASSSLQNSGVLEPSDSPKFPRKMGTNIGEDFHIDDQLPVPGTSEMSFRLDSNGVFQVYETAKSAELDTPIVAIPTLRDFYMDLDSILEISSDGPSKSFAFRRLQYLEGKFNLYYLLNEYQETADSKKVPHRDFYNVRKVDTHVHHSACMNQKHLLRFIKSKMKKSPDEVVLFRDGKHLTLKEVFESINLTAYDLSIDTLDMHAHTDSFHRFDKFNLKYNPVGESRLRTIFLKTDNFIKGRYLAEITKEVISDLESSKYQFVEWRISIYGRDIDEWDKLAAWVIDNKLFSPNVRWLIQVPRLFDVYKATGLMDNFQQVVVNVFQPLFEVTRDPTSHPKLHIFLQRVIGFDSVDDESKVERRVYKKFPFPKEWCTKQNPPYSYWMYYLFANIASLNVWRKQRGFNTFLLRPHCGEAGDTDHLAAAVLCCHSISHGLLLRKVPLLQYIFYLEQIGVAMSPLSNNALFLAYERNPFLNYFRRGLNVSLSTDDPLQFAFTKEPLIEEYSVAAQIYKLSAVDMCELAKHSVEQSGFEHIVKQKWLGSHYHLPGVAGNDMARSNVPSIREAFRHETLMGELAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAN3</accession>
    <name>A0A9W8YAN3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAN3"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="95" mass="9737" checksum="8AE464D14B5A3C8B" modified="2023-11-08" version="1">MLNPELGVGEDNAEGDAVRDGGGVEDVTGTAEPSSICAEEAVLVGLWGSWELKTGVSNDTVTDEADREVALGVEKMVDSIELGGRSTGVDENTVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAQ0</accession>
    <name>A0A9W8YAQ0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_004094</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAQ0"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="43"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="176"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="179"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="220"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4372320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="233" mass="25505" checksum="C06074E05B317113" modified="2023-11-08" version="1">MTASAIQGDKEKCTLAGMDDYLAKPVKGKLLEKMLVKWAIEGRRKTAKAEPSTNVDDQHGAATKATPAPIASISAKDHPQVPGSPAESETTAQALTTQLHRLHYQSDAAFARTTEDEGDRAMRRIQAEERDTKLRDDKLLSLVGPQLIRHNSSQGPAIEPSMPLTVENIEKLVHEQGDGDSSATRTLSSLDAGSNRVNQSHSRTDSRQQQLARPMLPEAQLHESEDTIVERNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAV9</accession>
    <name>A0A9W8YAV9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alcohol acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_004074</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAV9"/>
    <dbReference type="OrthoDB" id="3355480at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052058">
        <property type="entry name" value="Alcohol_O-acetyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037:SF1">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4372300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="590" mass="65196" checksum="AF8CDBD7CBF68099" modified="2023-11-08" version="1">MATQPKPQEHWLAKYASNYHAWKPSTTPSGQKCFQRPLGLVETSFDIDGNYYGGRADMTAIFTLALRHTLSKAELRRRIALAWTSLRLQHVLLMSRVADNVETAAAAGKKKSFVVDVPASKEDAVRDVESSIIWVEDLYGGEEAGVMDDEKELYRHALNVGRIIEPEKCMSKMHVLPLKKRPDGKTYELRFLIIMAHQISDGLSAYNWFSHFIRILNSPLSDIEAAISTSIAQEQIRKILPPAQEDLYPPIAGNKARQRWFWAIIRVLRHVKKPLPPTFVNPLCREEQQRLEKPVVAFEPKSSKLFDYSEAVRPPMNSGHVTASLSPTASQRLIHLCRSANVSIGAGCFALAGLSMMEIHESRYPDMPPASRPAFAASFPLNPRAFFANPPAADSCMLAFSEGIIMPFLPRSLPIEGRFRLVAKHANRELRVYQKRLKGNKGEALIPGLDKHSPGRLLATGYLAQIERANSKLPMEKQVDTSPQGALAPSISPYGATCGVSSVGSTAAFFKRGTYDLSNMEGEDLVVDFLDLKMGVRARDNEFLVGSSTNAQGIVGFGVSYDASAISEEAATMWKGKIEGLLEKSGAARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBR4</accession>
    <name>A0A9W8YBR4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004341</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBR4"/>
    <dbReference type="OrthoDB" id="3790073at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="530"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="654" mass="73065" checksum="B3CBC95F1EB43247" modified="2023-11-08" version="1">MKLSFTTGIASSNSTSGSSISSKRSRSNDTDSRIKDAGTARTQTTLTMWKRSKPTKKPVSSASNKSAEQHAVPSLTSSLNYRRQDPTSIATTFNSPKRYGSEQDNPEYSQEPKRAVSEPLKLVECNSAPNTTTILDEDVLFSDYDEPEPDSSTEDSIDSINSTVAIDLLASAQSPSMLQSASYFTELQQSIQRSLSSEWAEIEAFLHTDYVPQELEEFNDLFEAFRRQETGFSGWNPAFLVTPSGHPGERLHLKLHYPTFRSQHLEYGESADDTNNSTKLLMRKGLDASNAFWFEACFRRQTPTSSRHQECATKLWPTEVKDTHSSFSHLCEGFRKKVLLLFGAVNRKNFNLRYAVVERTVRTSPTELVTVGIVRRPNEAIHYLVVYCPHPEWLLWNWTTWAGRYYDACINVAATLTGLEVLPHLFEQRASCVKVNATQKNALRDADTTRKLEKQNKVRCHPDDLPPNVQLFAANNGIPKDRLKTHFQGGWSVAQLVYEFIKARQPSTSSWNNGVRMPRNLPRNLPAQPTSGLSTRSSSSTSSALCIVPNVTKSKNPIIKTAVIHKGQEILLMCIRCGKTPHSKPVDRKPVFVTISAAAGKYIAQRQACATPACNPKGRKGQIDQFMVPQDPNIPFIRSDNLSKVAIPEDTKTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCC1</accession>
    <name>A0A9W8YCC1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002503</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCC1"/>
    <dbReference type="OrthoDB" id="265717at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053185">
        <property type="entry name" value="SET_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332:SF2">
        <property type="entry name" value="SET-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="476" mass="54360" checksum="852D915760FB445F" modified="2023-11-08" version="1">MQSLNSGSMYALKDVLGKGKGIVAIENISKGTRILSEEPVITTPEYQQDDEWLKSHLSQQVDVLSEPQRQSFLSLHNLYPFQNIVEQSLGIIRTNALPIEADGIGGGIFLEASRINHACDNNAQKAWNQCIKRHTVHALRDIAKGEEITIYYLGLDSSREVRQKKLEDKFGFLCSCRLCSLTTELSQKNDEMLERIDYLDDLIGRDGMQMKFFLRTLRYVDERVRLYNEQGPGNSGLPRAYLDAAQIAIANGDLARGRIFAERAVEGWRIANGSDSKEVIRHGSLARNPATLPLYGLSMKWKTSLNEVPQELDLDDFEDWLWRRGKSNKLGQSGLLTDLHNREIFPGFTALPSSNPLDMKFYEVVDGTYQPLRHWCFLGEITDYTTLHHLELELTDIDEKKIPLHFYTDSRGSELVPAQIQKGYTVAVLYAQRRAFTYGDPGIRHEDPQKLKNVPWMQKEWRVLESMREVLVVLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCX9</accession>
    <name>A0A9W8YCX9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Vacuolar ATPase assembly integral membrane protein vma21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">VMA21</name>
        <name evidence="8" type="ORF">N0V83_004030</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for the assembly of the V0 complex of the vacuolar ATPase (V-ATPase) in the endoplasmic reticulum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasmic vesicle</location>
            <location evidence="6">COPII-coated vesicle membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the VMA21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCX9"/>
    <dbReference type="OrthoDB" id="160405at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03058">
        <property type="entry name" value="VMA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019013">
        <property type="entry name" value="Vma21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31792">
        <property type="entry name" value="VACUOLAR ATPASE ASSEMBLY INTEGRAL MEMBRANE PROTEIN VMA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31792:SF3">
        <property type="entry name" value="VACUOLAR ATPASE ASSEMBLY INTEGRAL MEMBRANE PROTEIN VMA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09446">
        <property type="entry name" value="VMA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="2 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4372256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="107" mass="11876" checksum="9EEACA9572EE178B" modified="2023-11-08" version="1">MTTRRIVSDEKSFLDNDGRGAPEPSNIAPAVPSSVIWKLMSFTFAMITLPISTYFFTVNFVFSGNATYAGGLAALMANVVLIAYVVMAFKDDQEEQREEAEKEKKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD24</accession>
    <name>A0A9W8YD24_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">COP9 signalosome complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_003103</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Component of the COP9 signalosome (CSN) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD24"/>
    <dbReference type="OrthoDB" id="422427at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000022">
        <property type="entry name" value="COP9 signalosome complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019585">
        <property type="entry name" value="Rpn7/CSN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045135">
        <property type="entry name" value="Rpn7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145">
        <property type="entry name" value="26S PROTESOME SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145:SF2">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10602">
        <property type="entry name" value="RPN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="10">
        <location>
            <begin position="243"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4374362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="462" mass="51982" checksum="B8EC476B990B15AA" modified="2023-11-08" version="1">MAASIEQSPFFQQQRAKGEVVVKDAPKFDLESYIANYAGITRIDRLHHIGSHSPYLAVDAYRLAIADAKKGKNVSRYTGLVEEFSNIAPQDAAALTDTAWAEKKTRETQQEQDRLEHELKSYKNNLIKESIRMGNEDLGHFFFDTGDYANAHKAYMKMREHCTSNKHLADMTLRLVYVSIAQKSWLSVQSNLAKVDAAQLKGDDKFKLDPIVSACTGLSHMAIGNYREAATNFLNTSPAYVTADPAANIVWQKEVLSPNDIAVYGGLCALASMDRSDLQNKVLAHTEFRNFLELEPHIRRAITLFCNSKYSACLEVLEGYRTDYLLDVYLSKVLNTIYGRIRTKSIVQYFIPFSCVTLAEMASKFPPTDHATIEDELEEMINNNTLDARIDLVDRLLVCPPTNPRYEVLEDALAMAEEYNHTLRLRLTRLNMLAAGMEIQPNKGARARRLRVVVGAMGLGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDE6</accession>
    <name>A0A9W8YDE6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cell division control protein 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CDC11</name>
        <name evidence="6" type="ORF">N0V83_001820</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDE6"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032156">
        <property type="term" value="C:septin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000328">
        <property type="entry name" value="Septin spn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="5">
        <location>
            <begin position="16"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="294"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="301"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4374744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="375" mass="42835" checksum="9C31460B1EA1A724" modified="2023-11-08" version="1">MSSALNKTRRKKNVKKGIQFCLMVCGASGTGRTTFVNTLCGKQVLGHKEVDDPTTAHVEEGVKIKPITVELDEEGTRISLTIVDTPGFGDQIDNEASFSEIVGYLERQYDGILAEESRIKRNPRFRDDRVHVLLYFITPTGHGLRELDIELMKRLSPRCNVIPVIGKADSLTPAELAESKRLVMEDIEHYRIPVYNFPYDIEEDDEDTVEENAELRGLMPFAIVGSEDIVEINGRRVRARQYPWGIVEVDNPRHSDFLAVRSALLHSHLADLKEITHDFLYENYRTEKLSKSVEGGAAADSSMNPEDLASQSVRLKEEQLRREEEKLREIEVKVQREINEKRQELLARESQLKEIEARMHREQSGQLQEHDAEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDX6</accession>
    <name>A0A9W8YDX6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003094</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDX6"/>
    <dbReference type="OrthoDB" id="3532430at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159">
        <property type="entry name" value="CENTROSOMAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159:SF31">
        <property type="entry name" value="CENTROSOME-ASSOCIATED PROTEIN CEP250 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1074"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="215"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="484"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="618"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="989"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1080"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1101"/>
            <end position="1117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1117" mass="127012" checksum="DBD4767FD932A004" modified="2023-11-08" version="1">MARLSGLPSEHHHEGPGDAPRRKRGRKPKKQTPSEDMTSTGKRTASPSAELSQTKRTRMQVDDDEDQIAEEIQQSFSRSQQGDTIRVETQTSTATTSRRPARRYSEPPITADDDDDELASRALPSTQPTSGLTPHLDRIGASHTRFTNKRRARMSMPAQLHIERVDEADEDGTQFQYAPLTAVLDSRTRRRLRRSHLSQEVNEFEDHQKQDKKMLLELRRQLRAQDDKIKDLEYRLEARRLGDIDLTDEHAEELEHQLAQAREEIDELRASSLYNGDDNDAVDMSDYEEDDLLLVNSEELHVSQDLEMDHTPNGKYASRVLELSNQVTFESLPGISQLSHDTLIEDEDTPVPDKIHDQAVERYERELQHYIHLLAESQGALRVMTLELQNLHVIEAGASTDEILVQLRHGFDTLRTEIEKYFPNTTNGLTNQQVLRKVPELFSGIFFELREKLAQISSSQKTEVLLRRQYEGVLDLLGESDERVEQLQKDLYNLDKTNEEKQRTILDLEERVTELTTLTKDQAGQITENDAQIHGLQDENEDKETALGRLREALEKYRKDLDSVEKSAIVFEEEHHVMIGRLEQEHADTVNELSAELGAETEGREAAEADALQKGEYIEELEGRLARMETDVDGIANEMTMLRERLATQTEARQVAEGERDEQAELAYEHANTVENLKETIMELKEDLAEALANLETERSQREKTEVALDDANGKIDDLNSRLHDAGLQANELRSKLFQIQQEKEATIAQLEKDAQEREDELNQQLADEEQLRDSAEATIAKLEEQLAQLQGSLATVEEDVVTMTESRDELAQDRDAQVATLNAQLADLKAKYTALENSTNSTITSLQANITDLNNQVQRQQVEIKRLVEEAAEKDRVYLEDTTALKEKIEVLEEDLDTQKVENEGYRKENASLSKRVENEAQELLNIVGAHSEQVKSLQTVISTQEATIKNLQEASAKRVTEFEETVEERTREITELQLMGDARVETITILEAQITTLKERFEAAEEDTRVTINALMLSQRQLQDQNEQLADALKGRNAEALKAVQEMKLKRVEVKTQGVDLHRVANGKVTKTSEKVKIGKKGRKKATKRQWDSGFGVDENIEDGEDVDGEEPIAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDY4</accession>
    <name>A0A9W8YDY4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M24 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_002139</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDY4"/>
    <dbReference type="OrthoDB" id="5876363at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01089">
        <property type="entry name" value="PA2G4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000029">
        <property type="entry name" value="Proliferation-associated 2G4, a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000016">
        <property type="entry name" value="Putative curved dna-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047113">
        <property type="entry name" value="PA2G4/ARX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804:SF11">
        <property type="entry name" value="PROLIFERATION-ASSOCIATED PROTEIN 2G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804">
        <property type="entry name" value="PROTEASE FAMILY M24 METHIONYL AMINOPEPTIDASE, AMINOPEPTIDASE P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="3">
        <location>
            <begin position="19"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="381"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="382"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="403"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4375059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="412" mass="44879" checksum="065DB7117FFCCDC9" modified="2023-11-08" version="1">MAEAAAVDYTLANPDTITKYKVAGEISQKVLKEVAGWIKADASIVELCERGDQLLAEEVGKVYKGKKVLKGIGHCTTISPSAYVTPYTPLKSDTEEAAATLKEGEVVKIQLGAQIDGFCTIVCDNVIVGASEGEVTGREADLILATYYANELLLRLMVPPGLVAHGDEEEQKKAASKKAYTQSQITNMLEKVTKAYGCNLVESTTIWLFEHNEIESKKKIILAPGEGVRGEGLPEIGEVWGVEMGVSLGSGKVKNNGNRTTLHRRTATTYQLKRPTSRSLLSEVVKKFGTFPFSLRQLEDEKSAKVGVVECVRSGVLRQYEVVVEKDSSPVARLFSTVAITKNGMQRLAQPAALDLSKYKSDKKIEDEEILKILEQPIGKTSTKKNKKKKKKPAKKAAAGGAAEEEEESDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDY7</accession>
    <name>A0A9W8YDY7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PRE2</name>
        <name evidence="12" type="ORF">N0V83_002381</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDY7"/>
    <dbReference type="OrthoDB" id="37597at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03761">
        <property type="entry name" value="proteasome_beta_type_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000013">
        <property type="entry name" value="Proteasome subunit beta type-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF3">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6 11 12" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4375295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="283" mass="31019" checksum="2F4A8F36CF3CB8C6" modified="2023-11-08" version="1">MDSLVAQYSRPAHADEGQTQDEQLELHSGTPELSLKFALPPVAQSASWLRAMTDDYSNPNCPIKIAHGTTTLAFRFKGGIIVATDSRATAGNWIASQTVKKVIEINNCLLGTMAGGAADCQYWLAYLGMQCRLHELRHKRRISVAAASKILANLVYSYKGMGLSMGTMCAGVTPQEGPALYYIDSDGTRLAGNLFCVGSGQTFAYGVLDANYKYDLEDDEALELGRRSILAATHRDAYSGGFINLYHVKEDGWVKHGFNDTNPIFWETKLKKGEFSNVTSELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEB5</accession>
    <name>A0A9W8YEB5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">WD repeat protein Lub1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">lub1</name>
        <name evidence="9" type="ORF">N0V83_001939</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEB5"/>
    <dbReference type="OrthoDB" id="10265988at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010992">
        <property type="term" value="P:ubiquitin recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.870:FF:000003">
        <property type="entry name" value="Polyubiquitin binding (Doa1 Ufd3) protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000236">
        <property type="entry name" value="Polyubiquitin binding protein (Doa1/Ufd3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.870">
        <property type="entry name" value="PFU (PLAA family ubiquitin binding), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015155">
        <property type="entry name" value="PFU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038122">
        <property type="entry name" value="PFU_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013535">
        <property type="entry name" value="PUL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849:SF0">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09070">
        <property type="entry name" value="PFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08324">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51394">
        <property type="entry name" value="PFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51396">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="10"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="105"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="233"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="PFU" evidence="7">
        <location>
            <begin position="372"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="PUL" evidence="8">
        <location>
            <begin position="500"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="464"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4374861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="770" mass="83509" checksum="F047B030B67A3F74" modified="2023-11-08" version="1">MGDFKLSATLRGHEDDVRSVAFPSPSSVVSASRDFTVRIWAQQSSSPPVWDSTIKTHGKEFVNSLAIVPPSSEYPEGLIVSGGKDQIIDVRQPSKGLDDDADALLIGHGNNVCALDVSQDGKYIVSGSWDMEARLWEVGKWGDSTVLQGHTASVWAVLAYDTKTIITGCADKQIRIFQTSSSGTPVRSIQAPEVVRALCRLPSNHPSGAQFASAGNDAVIRLWTLNGQQVAELHGHENFIYSLAVLPNGGLVSAGEDRTVRVWENNQCIQTITHPAISVWTVAVCSENGDIATGASDKLLRIFTRDQGRYAAAAEIEQFNDDVKGSSIPQQTVGDINKEKLPGPEFLTQRSGTKEGQVQMILESNGNVSAYQWSGAASQWVNVGTVVDSAGSGGRKISHNGKEYDYVFDVDIEDGKPPLKLAYNLNQNHYEAARKFIEDNELPLTYLDQVANFIIQNTQGATLGQGNAPGADPWGTDARYRPGDASQTTSQPPPAPPATKILPQKDYLPIATGNHKIIFKKLQEFNEALVNDGQKGISLNPSDVEQLSTTVIALEKGDGKSIDITGVELLVRAATQWPAEKRLPALDLLRLVLAFEEPAAYLVSPEQNLLPALTESGVFSDSSPPNNTMMAIRCFSNLLQTDKGRLLASTEFDTIHPHVAPFITSTNRNLIIALTTLYINYSVLLTSENNADRALSLLDDLSKILTTATDSEAVYRALVATGTLLCLGPDFCEAGRDILQIGDAVKRAEEKVKEPRIRNVIAEIRQKLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEL1</accession>
    <name>A0A9W8YEL1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001281</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEL1"/>
    <dbReference type="OrthoDB" id="3932250at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4376001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="89" mass="9401" checksum="0BAD8F7812FC5B19" modified="2023-11-08" version="1">MLACIATRVPITAAFAGKRAAPAAVRYNSSSSSSRAARVMEMAAQAERPSGAEAAVPVMWAVCGALTYTAWNRMTERNAGENVEKLLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YER1</accession>
    <name>A0A9W8YER1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Beta-xylosidase C-terminal Concanavalin A-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_002594</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glycosyl hydrolase 43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YER1"/>
    <dbReference type="OrthoDB" id="2139957at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041542">
        <property type="entry name" value="GH43_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006710">
        <property type="entry name" value="Glyco_hydro_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051795">
        <property type="entry name" value="Glycosyl_Hydrlase_43"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42812">
        <property type="entry name" value="BETA-XYLOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42812:SF12">
        <property type="entry name" value="BETA-XYLOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17851">
        <property type="entry name" value="GH43_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04616">
        <property type="entry name" value="Glyco_hydro_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-xylosidase C-terminal Concanavalin A-like" evidence="6">
        <location>
            <begin position="262"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity, responsible for pKa modulation of the active site Glu and correct orientation of both the proton donor and substrate" evidence="4">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606710-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4373855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="476" mass="53985" checksum="559D6E4A4DE571F3" modified="2023-11-08" version="1">MPLVRNPILPGFNADPSIIRVADDVYTDVKRKDGSFKDTPNYIVTADKIEGPWSDPIYVNSSGFDPSLFHDDDGRKWFVNMLQDHRRRPRAFAGIRLQEFDPVAEKLVGPAKTIFYGTELDLVEGPHLYKRNGWYYLLTAEGGTGYEHACTLARSRNIWGPYELHPEKYILTSKDAPLASLQRAGHGDLVETPNGKTYLVHLTGRPTTQARRCVLGRETAIQEAYWTDDDWLYIKNGPIPSLDVELPAERDENSYWAEHCYKFDTHEGLHKDFQWLRTPESERIFAVRDGLHLTGREAIGSWFEQALVARRQTHFSYDAETVISFCPTDERQFAGLTAYYCRYNFFYLTVTADAAGQRELLILTSEASWPDGKLHHPLANPIQIPNQGKVKLAMSIRGKDLQFSYSLEGEPTLKKIGPVYDASILSDECGGHQAHGSFTGAFTGVAASDLNGTKTTALFDYFLYRPMKHHSDRYDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFC0</accession>
    <name>A0A9W8YFC0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_002495</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFC0"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF28">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4375408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="12523" checksum="90881158530417B1" modified="2023-11-08" version="1">MYLYPAEIMPTCIRSAGVATAYVVMHTVMIVIVQVAPLALEAISWRFFLIFLVANVAFVGILYTFYPETKSKTLEEIGALFGDEVAETLEEAGLHFKEEKQQTQHIERPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFE4</accession>
    <name>A0A9W8YFE4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFE4"/>
    <dbReference type="OrthoDB" id="10265994at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="208"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="226"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="240" mass="25919" checksum="0A84788DC923EE74" modified="2023-11-08" version="1">MAARSRPRGGQLKDEFDEERSIWNSIKSDGKRVDQLMREAEAIRIRLGELNTEQDARIARGAEPSSRIDDELEKNLREDIRLCNEIQTLLQPIEGGNDICTQLNILSALRASEEMTTSASRATSVGGKSGRDRQGKRKLTDVDDRDSIAADSPGGPSPKVVISSQKDRLVAKSGSSRAGSVPVREGSVKAEDDKDDMGKDARPRLSPKPKSSTATTRRTAPPPAPTQATKAKASSAASRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFK5</accession>
    <name>A0A9W8YFK5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Glutamate--cysteine ligase</fullName>
            <ecNumber evidence="3 11">6.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Gamma-ECS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 11">Gamma-glutamylcysteine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">GSH1</name>
        <name evidence="13" type="ORF">N0V83_001438</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cysteine + L-glutamate + ATP = gamma-L-glutamyl-L-cysteine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glutamate--cysteine ligase type 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="3 11"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFK5"/>
    <dbReference type="OrthoDB" id="7939818at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017109">
        <property type="term" value="C:glutamate-cysteine ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.960:FF:000002">
        <property type="entry name" value="Glutamate-cysteine ligase Gcs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.50:FF:000001">
        <property type="entry name" value="Glutamate-cysteine ligase Gcs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004308">
        <property type="entry name" value="GCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164">
        <property type="entry name" value="GLUTAMATE CYSTEINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164:SF0">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03074">
        <property type="entry name" value="GCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="5 11 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="366"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="366"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4376157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="512" mass="58403" checksum="7B73171B8D430FE5" modified="2023-11-08" version="1">MAQTEFPITLTTFPRLGSVDCITPNYPVSGPKLRSQFVPDEIANPHIRFPTLAANIRWRRGRKVEVNVPVFKDENTPWPWKDPTVNYNLHQWPEDDDVRNGAAKDNFIHMDAMAFGMGSCCLQITFQAKNIEEGRKMYDQLSPLGPILLALTAATPVYKGFLADTDVRWNQISRAVDDRTPEELGEKPLKNDRWRLPKSRYASNSTYISQDPRLRPEYLDPDLIIDKDIKKRLVEGGMDDLLATHFAHLFIRDPIVVFAEDLEELDLTKADHFENLQSTNWQHMRFKPPPAGSDMGWRVEFRPMEIQITDFENAAFSIFIVLLTRAILSFNLNFYIPITRVSENMETAHIRDAVSTQKFWFRKNPFSSHKSTNGTGTHTPAEASSRPATPTGPVEDEYELMTINEVINGQTSTDGGFPGLIPLVESYLNSMNVDVETRCDLATYLDLIRKRANGTYWTAAKWIRHFVRTHEGYKGDSVVSDEITYDLIKAAEQITNLEGRDGLGKEMLGTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFX5</accession>
    <name>A0A9W8YFX5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_002232</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFX5"/>
    <dbReference type="OrthoDB" id="189226at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036149">
        <property type="term" value="P:phosphatidylinositol acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07990">
        <property type="entry name" value="LPLAT_LCLAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032098">
        <property type="entry name" value="Acyltransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF16">
        <property type="entry name" value="LYSOCARDIOLIPIN ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16076">
        <property type="entry name" value="Acyltransf_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="6">
        <location>
            <begin position="130"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="503"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="631"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="509"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="539"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="656"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4375148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="709" mass="77596" checksum="67EA0DB97A053A27" modified="2023-11-08" version="1">MSTALKKKKKDDLGAGLRVSKDNIAAHPAGPPRYGELTQAERAFTLASTFLSGVLAISASQFLGAPLKLIDPQFYDGYMAYTKECFAILITCLTQWWAPTVVRVSGDSSMVGQLIKKKDGSLQCNFADRMVLMANHQLYTDWLYLWWIAYTNNMHGFIYIILKESLKNIPIIGWGAQFYNFIFLSRHWEEDQRTFKKHLAQLNKPNDPMWLIIFPEGTNLSASTREKSKKWAEKNGLQDMKHQLLPRSTGLRFCLNELRETTEWLYDCTIAYEGVPPGQFGQDIFTLRSSFFEGRPPKSVNMHWRRFHLDTIPIQNTKAFEVWLRNRWREKDYMLEYFNRNNRFPAEDFWKNHLDMSSQSSHNGNKSLRSVPRPAVQIETEVKSGNWNEFVKIFAPITSVMMALTVAYGASPDDLPIPGGKEFFEQHIKSLLGQGGEMKGLPSPEQLEKMIEGAAQMGAAQAAEPQKLTGAQKLTQENLAKLVKETAISSGVVLPGGMRRASTALPQAPTKTLATPTVSVPRKASTVVNGPTAAKKKPTAGATAKPKPGTAAHARANATATKPKPAAPTGPMKEMMTASGVMIKVPKSEVEDAKKTGTITTKSGLKIKIGKEDIEEAQKKKGTTFVTSSGVPIKVTPSGAITVPEKAGAQKPKPTPTANKPAAPKVVPAAAAKKAGAAPKLKGPVTATKKKAPVAAGAGTKPSAAPKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGM5</accession>
    <name>A0A9W8YGM5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PHO89</name>
        <name evidence="9" type="ORF">N0V83_000108</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Sodium-phosphate symporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGM5"/>
    <dbReference type="OrthoDB" id="260807at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101:SF80">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="457"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="546"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="299"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="299"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="339"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4377284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="580" mass="62146" checksum="A4FFD133E080C89E" modified="2023-11-08" version="1">MPALAQFDYLFAIGTIFAFLDAWNIGANDVANSFATSVASRSLSLKQAMCIASVMEFAGAMLVGSRVTDTIRTKVISISLFEEDPSVLMLAMVCAIVGSATYLTIATKFTMPVSTTHSIMGGVIGVGIAASGTKGVNWSFKGVSQVFAAWGIAPGISAGFGAIIFLATKYGVMRRSNPVKKAFIMVPIYFFITAFLLALLIVWKGGSAKIKFTDAEAVGVSFGVGGAVAIIVATFFIPFLYRRIIKDDWELKTWEVIKGPLLLRRAEPPVRPEGVTAGHIKDYYAGHLTSGQLEAKRAAEAHVAPDDIEKGAGHSVKPTGDGAESDITPIGSVRGAPVTATSHQQLSTPKKQPPPGAWYTPAVAFYWVKYAVFHGVEQDVVDQQSKRDFLSGDIEKVHATGEHFDNRAEYTYSFLQIMTASTASFAHGANDVSNAIGPYTTIYFIWSTAKISNKVPVPLWILAFGGAGIVVGLWTYGYNIMRALGNKITLHSPSRGFSMELGAAVTVILATKLSLPVSTTQCITGATVGVGLCNGTWRTINWRMVAWIYMGWFITLPCAGIISGCLMGIILNAPRWGMGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGS2</accession>
    <name>A0A9W8YGS2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Actin-like protein ARP6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Actin-like protein arp6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ARP6</name>
        <name evidence="8" type="ORF">N0V83_001706</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Involved in chromosome stability.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the SWR1 chromatin remodeling complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the actin family. ARP6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGS2"/>
    <dbReference type="OrthoDB" id="6220758at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10210">
        <property type="entry name" value="ASKHA_NBD_Arp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000014">
        <property type="entry name" value="Putative actin-related protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.36.70">
        <property type="entry name" value="Actin, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4376422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="439" mass="48793" checksum="BB4A55AF0B3B4693" modified="2023-11-08" version="1">MPPKGSKKSAKATSNKTLVLDNGAYTLKAGLVSTDTKPTYDDCAVVPNCIARSTRDKCTYTASELASCKDFGELAFRRPVEKGFIVNWEAEKAIWEHEFMGPTAGEGLRCDPKETNLVLTEKPNCPKELQKNCDEIVFEQFEFAAYYRCVGPTLNAYNTTNAASISSLPQECLLIIDTSYSDTTILPLYNGKLIQSAVRRLTVGGKLLTNYLKELSSLRHYNMMEETYLLNEIKEAVSYVAPSSQQYAKDLERTWKGRLGDRRELDTSIVMDYVLPDYEKSIHGYARPHDAAKSRMRRGLQPMQGPREDLLPLGNERFAVPELLFNPSDIGIQEVGLPGAIVDSLSVLPEALKVGMLANVVIVGGNALISGFTARLEAELRALVPAEYMLKVSRAEDAIKHTWLGGARFASQPELLKQVLVDKAEYDERGSTWLVKKFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGW1</accession>
    <name>A0A9W8YGW1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001766</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGW1"/>
    <dbReference type="OrthoDB" id="10635389at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="135" mass="16250" checksum="C8EA54DEB8B7324A" modified="2023-11-08" version="1">MIWRRFGIFGPVGSVVKYASYRWFHNDQDDKIAHLSRTVRQHFDELIAAPIFFDGEYVETAQEKGKDDKVIEVDWHEFSKWYDEVISWNEMHNEMEREREQKEDEKKKKKKGRKKKEEEEKRARKGGGTAQEKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGY3</accession>
    <name>A0A9W8YGY3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000346</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGY3"/>
    <dbReference type="OrthoDB" id="94998at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35606">
        <property type="entry name" value="CELLULOSE-BINDING FAMILY II PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35606:SF4">
        <property type="entry name" value="CELLULOSE-BINDING FAMILY II PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="237" mass="26893" checksum="ADF03F603C65C177" modified="2023-11-08" version="1">MVKVWTQTLKENPQGLEDKNWITDQMMANNGSINYCVRWNYSGKSTAAARASTVTALQRSLQKWFDVLVGFEGFPLTKLDVNVVGYAVKDKSLIEGSTTDLDIYTTVDGDGVPECDTRCYRAAHLDGDLSQCPGGEDSRYDISLWLDESLLGQMAGYGYNWGEEFAPDYFLDNVDTENIHILLHEMGHGFGLLDFYDWVPEGQTSFVMMAGSAMEITEFDAWMLRDWWRKLKAARSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH04</accession>
    <name>A0A9W8YH04_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Potassium transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TRK1</name>
        <name evidence="12" type="ORF">N0V83_000750</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TrkH potassium transport family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH04"/>
    <dbReference type="OrthoDB" id="9999863at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140107">
        <property type="term" value="F:high-affinity potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004773">
        <property type="entry name" value="K/Na_transp_Trk1/HKT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015958">
        <property type="entry name" value="Trk1_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051143">
        <property type="entry name" value="TrkH_K-transport"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00934">
        <property type="entry name" value="2a38euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064:SF30">
        <property type="entry name" value="HIGH-AFFINITY POTASSIUM TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064">
        <property type="entry name" value="POTASSIUM TRANSPORT PROTEIN DDB_G0292412-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002450">
        <property type="entry name" value="K+_transpter_TRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="4 10" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="387"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="460"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="523"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="556"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="585"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="640"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="669"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="88"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="264"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="766"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR002450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4377920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="823" mass="92161" checksum="EBE4FFA1FAEEC249" modified="2023-11-08" version="1">MALVASVILYGNGSIHYIDALFFGSGAATQSGLNTIDVNKLALYQQVTIMIIACMCNPIFINGSVVFVRLYWFEKRFQHVVIESRTNKSGRTRTISRRKSSVNAESAHDLGREERGVGDREIRVVRGANGHADGGTIEDEKAFQEGEDNAGIRKASSSDEDEVGPLDQPTAPVHHVTSNVVWADDLNAPAARERQRLRDSVETPEDRLPQVDKEKSIAFVESQRNPTEKAKLRIPGPRDFDRGLVPEQVGEHDEHLNRVISRMSQDSYPRRERSNSVPAMNGDDRPFKHHITIDVPDRPRPTTGASVYNRAKTTDMETPIPTPGMHMRSRSRSRTLASFLTRTKEEDEDPMPYLSWTPTIGRNSNFVDLTEEQREELGGIEYRALKLLAMIIVTYFVGFHLLGMVCLTPWINRDARFRAVVEDDGLSPTWWGFFTAASMFNDLGFTLTPDSMVSFQYAALPLILGTFLIIIGNTGFPCMLRFVIWICSKLVPRNSGVWEELRFLLDHPRRCFTLLFPSKANWWLFWVLILLNGIDLIFFIILDLNDPTVTTLPGGYRFLAGLFQAAATRTAGFAVVNIADLHPAIQVSYLVMMYISIFPIAMSMRQTNVYEEKSLGVWAGEEDEQDAHSSYLSHHLRRQLSFDLWFVFLGFFLIAIIEGSRLENTNEYAFSLFSVLFEIVSAYGTVGLSLGIPGFNTSFSGQFKTLSKLIIIAMQIRGRHRGLPYALDRAILLPSESLHQKENEDATRRARRGSYAFDIGGVGGGGGMDGITRTGTGLSRRSSVSGEQGRPSAGDRFNPKQLKRILSGAFSAGPSVQHREKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHI8</accession>
    <name>A0A9W8YHI8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000872</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHI8"/>
    <dbReference type="OrthoDB" id="201656at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08267">
        <property type="entry name" value="MDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050700">
        <property type="entry name" value="YIM1/Zinc_Alcohol_DH_Fams"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11695">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11695:SF294">
        <property type="entry name" value="RETICULON-4-INTERACTING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="1">
        <location>
            <begin position="17"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4378042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="338" mass="36296" checksum="ED96F6F262F5AFC1" modified="2023-11-08" version="1">MAQQTMKAWQYNSTKGGIEKHLKINNAAPLPTITDNELLIQVHAVGINPFDHKVPESGMPMRLLGSNLTPCVDFCGKVVKVGTKVDDLEVDKWVFGAKMAALNHGALAQYVAVPRQMVAGLPEGVKVEDAAGVGIVGLTAVQTTKPNVKKGDKVFINGGSGGTGVFGIQVAKALGCHVTTTCSTPNVELCKSLGADEVIDYKKDDIVQTLASKGPTFSLVVDNVGKPANLYTRASSFLLPQGKFVQVGSNPNLGGIAQISRNMLLPGFLGGGMHSYQVFFAKKAVHDLEQLRDWMKEGKVKGVTDSVWEWEDAPKAFEKLKTGRARGKIIIKVKQEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHR7</accession>
    <name>A0A9W8YHR7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000684</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHR7"/>
    <dbReference type="OrthoDB" id="3758401at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="217" mass="24204" checksum="E35901E3A481F6E1" modified="2023-11-08" version="1">MSDPMDIEPTIMDDMINETTVMDLDDDSMNLPSVTSFSNEYENLPAGGPVSHAHIINLDEYAGCIRETGAVQLQDAVVTTLFKTSPDTLKSFLDPTVNSFKAVFGTDDMQLVVWRKGVEVLIGKFEHHKSLKVYGFEHVAGFLIGYDGSWNLKVIARNAYCNAKWTDVWAGKFDSYGGLVKMYIESEELGMAKTSSTLAESILENMYWEFKANIKAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHR9</accession>
    <name>A0A9W8YHR9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001377</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHR9"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044283">
        <property type="term" value="P:small molecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000030">
        <property type="entry name" value="Thymine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00682">
        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="3">
        <location>
            <begin position="189"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4376096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="343" mass="38358" checksum="84EBBA0B38A0EF6B" modified="2023-11-08" version="1">MADIKVDSAVSQDDLVIPLIDFSAFLSGDPSAKKQTANAVLKGFQNAGFIYLKNHGISPSTVSDIFSQSAKFFARPKEQKDTLAWYSPAANRGYTAQGREKLVVLEETGTEEEMRKIVPDLKESLEIGRDDEPETPNMWPSGDEEAKEFKKEMMTFFETCRSLYTEVMRAIALGMGLEESWFDGFTDAGDNTLRLLHYPGVSKSIFKRNDGQLQVRAGEHSDYGAITLLFQDARGGLQVRSPKGTFVNATPIENTIVVNAGDLLTRWSNDTIKSTLHRVVEPPPRPEDADKDEYPPRYSIAYFCNPNYVRMIEAIPGTYEAAGRKYDNVTSGDYLIQRLTATY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YK86</accession>
    <name>A0A9W8YK86_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_000637</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YK86"/>
    <dbReference type="OrthoDB" id="332281at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009316">
        <property type="entry name" value="COG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024602">
        <property type="entry name" value="COG_su2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12961">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12961:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06148">
        <property type="entry name" value="COG2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 2 N-terminal" evidence="11">
        <location>
            <begin position="28"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="112"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="177"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4377807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="286" mass="32169" checksum="2C5213B54326F008" modified="2023-11-08" version="1">MSRFYFGDSRSNSSDDEDNLDTLPFATPLQRSDFLAPNFSPSTYLSTLSNRHQTLEDLRSELRSRSQLLSKELLDLVNSNYQDFLNLGNSLHGGEEKVEEVRVGLLGFRKEVNSLRDVVEQRELEVAQLLQERRDTRQKIEVGRRLVDYDARLKELESELVIESAGKDTIILGGEVSDSDEDEDEDEEDEGSYGVSITKLRRNVVQYQLILGLQKTLSEHPLITAQAPRIAKVRSTLLMDLSTALQQAKNAGAAGSGRIMKIMKIFADMEESAEAVKVLKSLKTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHA1</accession>
    <name>A0A9W9CHA1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Uracil permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010355</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHA1"/>
    <dbReference type="OrthoDB" id="2018619at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045225">
        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618">
        <property type="entry name" value="NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618:SF0">
        <property type="entry name" value="PURINE-URACIL PERMEASE NCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="288"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="332"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="354"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4362262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="513" mass="56101" checksum="BAD23FE3AA144EC5" modified="2023-11-08" version="1">MATADSKTFYDDKNGSPANSDYVEPTEVGQVSRRRRLPGFSLEVRQEESSFAATKNASNADFDPIPPSKRTWNWGAYVAYWMADAWAVSNWEVVQTTTGGQCMTVLLTAIWPSFANIPNHIPDGEGIKTNGMIGFVLYFLLQLPFLCIPYTKVQYFFAFKSVIAPIIFLAVFGDTLHKAGGSISSSTVISQGTTVSGSVLAWAFFANLNGVLGNYATLGLNIADFSRYANKPSAQNVQAFVIPVIFTIVGLLGIFTAAAAQSAYGEILWNPIDIISLWMKSGSHGGRAAAAFGAIGLIIVTLGINISANSISAANDLMAFCPRYINIRRGQLLAAFIGSWGFVPWKILASAAKFLAFLGGYTIFLGPMTSILMTDYYIVRRGNVSVPDMYNFHGMYRYSPKFASNWRAVVAFFIGCIPPLPGFVNNIVVAGNEKTTVSLGGQHLFAIGYIYSFVAAGVFYWLFNRFFPHTPSIMDHPETGEDIIAANDAKNVEKKRAVRAERRTSVVQKIFEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHH5</accession>
    <name>A0A9W9CHH5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">superoxide dismutase</fullName>
            <ecNumber evidence="4">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SOD4_2</name>
        <name evidence="8" type="ORF">N0V83_010598</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHH5"/>
    <dbReference type="OrthoDB" id="159229at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000007">
        <property type="entry name" value="Cell surface Cu-only superoxide dismutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4362504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="329" mass="33666" checksum="932F13B464FCE327" modified="2023-11-08" version="1">MLGYIRHWLLTPEVNIPNIERLIHGVTRTRPLNTHLHHDHAQISPPPPGPPAPRPLAISSQVQEAPSTPVLLNSTKSGVIPTLPTATPFTGVDTLQGAIISPLPPLPGYTGLLGTATAQTAQPTSTYLASLPPTMFNPLVGTVVQGSLMAVGGPSGVTFTVNITNLPPQAQYGPFNWHIHTLAVPADGNCTATLGHLDPTNRGELYMCDASMPATCQVGDLAGKHGGKIMTEGSFSTSFVDPYLSVVNGSAGFFGGLGFVLHSANTTRITCANFEMMGASGGGNASGTMSMMPSSTGTGAMPQYTGGVARVGAAGVGAVGGAVVALLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHI9</accession>
    <name>A0A9W9CHI9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010656</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHI9"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF223">
        <property type="entry name" value="CYTOCHROME P450-DIT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="494"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="571" mass="64462" checksum="9479C1025A507DF8" modified="2023-11-08" version="1">MMYTLLAALPILFFIARRITNWTRNYISALKYGLPIILLPVSFEDPWWLLLRPLFSFISYLPFNLGHWYLYTTMGWPQEDSNRSLLKYGENFVLCSPRSNILVTAEQGVLEAVWGVHGKGWGLQEDQGQLFAFYGQNVSSTNGEMWRRHRKITGLAFNEATMRSVWDETSRRAGKLSLGDADGKESKGETLGNVRKTFDLLAMQILTVVGFGQNTDLTDVPPGHQESLMESLAFILQHIMLTVIFNSLKAPDFLLPNALRRLKVSLKELRVYMQESVLQHLQSVKARPEGVKGVSLLEAMVRANEASKQEQKTGGPRSYLSDSELYGNIFVFNVAGYETTASSFIFSLSYLAAHPEIQEWIREELDMCYNNSRGSDLDTTVPDYSETCHKLVRCLAVMNETLRLASPAPMFVRQPFAPTELLVNTPQGRRSITVTTDTQVGFNPYGAHLSPQWGPDNLSFDPKRFIQTSSSGEERLVVPEGPLFAPFMLGPRVCPGKKFSQVEFVAVLARVMTDWRVEVVRDPGESEELARRRLLAVVDDKVFHISAHLKRPEAAALRFVRRGLQCEGACF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHV6</accession>
    <name>A0A9W9CHV6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHV6"/>
    <dbReference type="OrthoDB" id="446723at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF81">
        <property type="entry name" value="PROTEIN ABHD13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="75"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="269" mass="29478" checksum="07CC2F9594C7A19E" modified="2023-11-08" version="1">MYQGAIAYLDGYNYHFSYPCGKNSLLTEKLFAWLIAPLGVYARHVDDFLQDQSAAEDIEARLAFKMLKNDPDARLLIYFHGNTATIAQQRRTEEYRSYASGASEKIFILAFDYRGFGASTGTPSELGLLNDAAAVLEWALNRARIPPERIVLLGHSLGTAVVAGIAHHYSKLDIAFAGLILCAAFTNAVHAEDDQTMPWNQTEELFRCALNAANESVLDNEAGANDGTKVIDLGEAGRQEVWQNGTRSISMTIAKHGGTYSLPGIKVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI42</accession>
    <name>A0A9W9CI42_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pre-mRNA-splicing factor ini1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ini1</name>
        <name evidence="2" type="ORF">N0V83_009907</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PHF5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI42"/>
    <dbReference type="OrthoDB" id="10248186at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005345">
        <property type="entry name" value="PHF5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13120">
        <property type="entry name" value="PHD FINGER-LIKE DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03660">
        <property type="entry name" value="PHF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016468">
        <property type="entry name" value="PHF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="108" mass="12239" checksum="BDEC4C3AD2CF956F" modified="2023-11-08" version="1">MSRHHPDLVMCRKQAGISIGRLCDKCDGKCPVCDSYVRPTTLARICDECSFGNYQNKCVVCGGEGISDAFYCFECTRLEKDRDGCPKIINLGSSRTDLFYQKKNFRNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIK7</accession>
    <name>A0A9W9CIK7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Carrier protein ymc1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">YMC1</name>
        <name evidence="12" type="ORF">N0V83_008250</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIK7"/>
    <dbReference type="OrthoDB" id="409586at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF12">
        <property type="entry name" value="MITOCHONDRIAL ORNITHINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="17"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="114"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="215"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4365630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="305" mass="33245" checksum="888E7879A66DDEA0" modified="2023-11-08" version="1">MSKPHLEHDVPEQGGLKRTLKDLFAGAVGGVAQVLLGQPFDIVKVRLQTTSQYTGALDAATKIYQNEGALAFYKGTLTPLIGIGACVSVQFGGFHYARRAFEARNTAKTGSPQLSYAQYYAAGAFAGIANTVLSSPIEHIRIRLQTQPHGANRLYAGPIDCVRKLSAHQGVLGGVYRGTAVTFLREAQAYGVWFTTFEYLMNADAARNGVARDQISTWKVAFYGGLAGEMLWISSYPFDVVKSKMQSDGFGADQKYRSMRDCFAKVWRAEGMRGFWKGIGPTLLRAMPVSAGTFATVEVTMRLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIQ5</accession>
    <name>A0A9W9CIQ5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytosolic factor, phosphatidylinositol/phosphatidylcholine transfer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SEC14</name>
        <name evidence="3" type="ORF">N0V83_008385</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIQ5"/>
    <dbReference type="OrthoDB" id="1434354at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051026">
        <property type="entry name" value="PI/PC_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657:SF1">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="106"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="348" mass="38897" checksum="88E178939BC9532E" modified="2023-11-08" version="1">MAAPHELKLDPKYDDYDYPTVAPAAQSGHPGHTTPEQDAQVFQLRSMLEQAGYTKNLDTLTLLRFLRARKFNVELSKKMFVDCEKWRAEYAGVGVEELVRNFDYKERPEVFEYYPQYYHKTDKDGRPVYIEQLGKVDLTALAKITSQERMIQNLVCEYEKMADPRLPACSRKSGYLLETSCTIMDLKGVGIAKATSVYGYLQAVSAISQNYYPERLGKMYVINAPWGFSGVFSVVKKFLDPVTSAKIHVLGSGYEKELLAQVPAENLPKAFGGKCECEKSCQLSDAGPWWDPQWAKEPKWAKKSDGDAIDNTKLPPPTHEGGAPAVPVGIDPATAPGSMPQSQTQHQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIV4</accession>
    <name>A0A9W9CIV4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008611</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4364995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIV4"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="138"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="453" mass="51888" checksum="7B7D298142509D73" modified="2023-11-08" version="1">MHSHRAPSAGPHYEFDDMMESNIDLGANQSTYDNDSYLQSGQQHSSFYDQSPTEYGNQYTSQWPTTSIAQQRFAHQQHLSALSYSSGSSGFRSSGNSVFSHDPTRDSTFSSISTSSYASDVPRDYYISQTEYLLTHSSSAQSSYTPSPVEGPTSPAPRKRTAQRQAAQEKDYFKTCISGNKQRRPCGKEQKYFCTACEKPFVEKADWRRHEETYQERPEMFQCDLCPAIYFLDKDFATHHVSNHRCAACFENIKCSQKRHVQLAKKQRVTRTGWGCGFCCHFSSSWTERCNHIAYHLEKEDKKVEDWRHSCVIYSLLHRPALLPAWTYILQSKRRHFTSFAWNQHSTGRVEGYPESSQLIQLQDALEYYTPNQNAAALAQLAFDKAKKQVALPEEDIAPPVPPKDTRDYRDHHATSLQDLMKETESWTQFVNSIVEDDIFPTGVCHLEGRHAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJD8</accession>
    <name>A0A9W9CJD8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-dependent Clp protease proteolytic subunit</fullName>
            <ecNumber evidence="7">3.4.21.92</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_008266</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase S14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.92" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJD8"/>
    <dbReference type="OrthoDB" id="2017408at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009368">
        <property type="term" value="C:endopeptidase Clp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07017">
        <property type="entry name" value="S14_ClpP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000002">
        <property type="entry name" value="ATP-dependent Clp protease proteolytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00444">
        <property type="entry name" value="ClpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033135">
        <property type="entry name" value="ClpP_His_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018215">
        <property type="entry name" value="ClpP_Ser_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001368">
        <property type="entry name" value="PRK00277.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009205">
        <property type="entry name" value="PRK12553.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381:SF11">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00382">
        <property type="entry name" value="CLP_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00381">
        <property type="entry name" value="CLP_PROTEASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0720">Serine protease</keyword>
    <feature type="active site" evidence="5">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4365646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="254" mass="28523" checksum="F7B046C50968CC86" modified="2023-11-08" version="1">MATILRRAARPSILRGFCRNDVRSASRKFGFAPQWSPQQQQDQQSPRALSLPIPYVTETTGGGWKTSDIFSRLLQERIICLNGEVDDFMSANCVAQLLFLEADNPEKPISMYINSPGGSVSAGLAIYDTMNYIRCPVSTICMGIAASMGSLLLAGGAEGQRYILPHARVMIHQPSGGYRGKASDIADHAKEILRIRDKLNRIYQSHVTKKRTLEEIEKYMERDYYMDAQEAIDFGIVDRILEKREAAVKDEEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJH3</accession>
    <name>A0A9W9CJH3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_008491</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MSOX/MTOX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJH3"/>
    <dbReference type="OrthoDB" id="2219495at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051698">
        <property type="term" value="F:saccharopine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008115">
        <property type="term" value="F:sarcosine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045170">
        <property type="entry name" value="MTOX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961:SF24">
        <property type="entry name" value="HYPOTHETICAL FRUCTOSYL AMINE:OXYGEN OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961">
        <property type="entry name" value="PEROXISOMAL SARCOSINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="6">
        <location>
            <begin position="9"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4365869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="433" mass="48265" checksum="781663F7E8474A49" modified="2023-11-08" version="1">MAAESKSNILIVGAGTWGCSIALELARRGHTGIKVLDGCSFPSAISAGNDLNKIAEEANKPSATDSNESYFWNRITEIAMHAWKRDPLFAPFYHDTGFIMAAASDEAYERCVEYAETEDVDISPLNSREDFQRTMPKGVLQGNFPGWRGFWKQGGAGWVFASGALQAMHDEAARLGVQFITSELEGEIQELIFFHDAVLGARTSDGVIHAANQTILAAGANSSALLDFKKQLRPTAWTLAHFPLSSEGAQLYKSLPVLYGVDRGFFIEPDIERHEMKICDEHPGYINPVIDSNGEMHSVPFAQRQIPKEAETRMRRLLTETMPQFAERKFSSARICWDADTVDRIFLIDKHPELRNLVVAVGGSGNGFMASPAIGILVADVLENKGEIEERVRRMTRWRPETSVDRDWWSTQGRYGADGKVMDFRDVAEWTNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJY3</accession>
    <name>A0A9W9CJY3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pectate lyase</fullName>
            <ecNumber evidence="10">4.2.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_007066</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Pectinolytic enzyme consist of four classes of enzymes: pectin lyase, polygalacturonase, pectin methylesterase and rhamnogalacturonase. Among pectinolytic enzymes, pectin lyase is the most important in depolymerization of pectin, since it cleaves internal glycosidic bonds of highly methylated pectins. Favors pectate, the anion, over pectin, the methyl ester.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Eliminative cleavage of (1-&gt;4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.</text>
            <dbReference type="EC" id="4.2.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 10">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the polysaccharide lyase 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.2" evidence="10"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJY3"/>
    <dbReference type="OrthoDB" id="441042at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030570">
        <property type="term" value="F:pectate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004898">
        <property type="entry name" value="Pectate_lyase_PlyH/PlyE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33407">
        <property type="entry name" value="PECTATE LYASE F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33407:SF9">
        <property type="entry name" value="PECTATE LYASE F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03211">
        <property type="entry name" value="Pectate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041017208" description="Pectate lyase" evidence="10">
        <location>
            <begin position="24"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4367483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="256" mass="26103" checksum="97FE86FBD6B4B86E" modified="2023-11-08" version="1">MKASVAASILAFAAAAAAGPARSSPMNFFSLTKRASLPIPDSTGTETLSEPQEISGTFDGGLKTYGRGVSCTGQAEGGDSDAVFLLKDGATLKNAIIGADQIEGVHCEGSCTIENVWWAAVCEDALSLKGDGDATVTGGGATGAEDKVIQHNGAGTVTIDGFTVDDFGKLYRSCGNCKESAERHVIIKNVKASNGKLLAGINTNFGDSATIDTATCATSVKEICEEFEGTTPGNEPESVSTGPSTACAYTDPLPEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK30</accession>
    <name>A0A9W9CK30_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_008131</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK30"/>
    <dbReference type="OrthoDB" id="3934656at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF232">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4366495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="23337" checksum="52E7EC26CE9C77DC" modified="2023-11-08" version="1">MQYEGKDEPGMFSSLHSYLGYTASVGIMNEFHKTLFPLFLITGSGGMFQMMKFTQDQISKFKSEADRDEKPTKGDFLSRMIKMHKENPEKFTDNDLFTACITNIGAGSDTTSISLCAVLHCLMTHDAAMAILRKEIEEFVDGSTENGRRISFQTAQKMPYLQACIKEAMRLHPATGLPLSRVVPKEGAIIAGRYFPAGVRTLPKKSTLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKE4</accession>
    <name>A0A9W9CKE4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007557</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKE4"/>
    <dbReference type="OrthoDB" id="5362630at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052973">
        <property type="entry name" value="Fungal_sec-metab_reg_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35392:SF3">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35392">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="768" mass="86282" checksum="8ABE05F25B62CF4A" modified="2023-11-08" version="1">MNNGPALPHDPNQRHNRRGKLVPVTTWHWVPGTDADDDLQHDQMPPTASVDYAQPLITPVNLQQTHNPYSPQIHTGPIHPTGHRYNGSVAPSASDLFNLAIAQTNAQPQYPQPDMIQFPDYSQAFTTSSNLSIPFENHYNPEAPYRADNSITPFRDDPTITAGPTSAWPSSVSGTLRNAFDGVYNLEMPPDVDPVWIEGNGPPANSQNSSAHLMAPFGDSPSLYSDIIDTFLANIQEASAIGDATDEERINGSISPFSQSTTATKNVSPQTTIPTPQSEITSSDLGSQIDSAGRTLRKRARSPGAVKHEKIIQNEGGKVQGMTMVWQGQKAPNRKLDDVTKADRKRRKNLGTCSRCKRLKQPCKGFKESPYLPCIACRTSNPTTITSVCLLEASLLNIALFRMGPPVDNPFHPLNFFSHRQNILCDVAKGDDTSNTLELELMQDDVGQMLTLTVSRFAPLPGDKVAYEGIGEEGPYKLDMPPYCISDIKKAQQSLITYMRKAKSAYFNRFVDRSNALLFNTFKMAAMCDFTKRPLINKALDIWAATRLIERTWKMCGSETLGIHPVKGRSDPWKGFVPVTPIMDQQLDQVVIREILVPRRNQLLAQLKEKVYNEYNRKLHAFEVYMTFFILLNNAEVQIAFEREFAQRYGFSGRFGPRGKYMDAEAQFHAARTMLGHFHYICRANSLLTTSPEFLKELGIGDTEKKYLEFVRRQVELQAPHFTKLVQEHRYETTMYFCHQMLFNEWKPGYGHIEELPEMTVGEVMAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKQ1</accession>
    <name>A0A9W9CKQ1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_006394</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Aromatic compound metabolism; phenylacetate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKQ1"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000072">
        <property type="entry name" value="3-hydroxyphenylacetate 6 hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050364">
        <property type="entry name" value="Cytochrome_P450_fung"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46300">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46300:SF9">
        <property type="entry name" value="P450, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0349">Heme</keyword>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4368039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="527" mass="59844" checksum="6D33F1E7C1F83D0C" modified="2023-11-08" version="1">MISTIVPLLSSQLSSHPLRSLLLLLLSIPITYILANEYVRFSRRIKGFSGPTNWPLVGNIPDIKYNAAEKYREWSRRFGAVYQIQLGNEPVIVVNSAEAARKIFGGNSQALSSRPVFWTFHKVLSNTAGTTIGTSPFNDSLKRRRKGAASALNKPSIATYIDHLDLETKDFVKDGFESGRAGTVGVDPMPMIERLSLSLALTLNWGTRMGSRHDPMFHEICEVEAAISKFRSTTGNLQDYIPILRLNPFSFGTRAAKEYRNRRDVYLTRLNRDLDDRMEKGIHKPCIQANIIQDKEAALNKEELTSISLTMLSGGLDTITTLVQWSVALLAQRPDIQKKAIEEIRKFYSDEEPLADAYDDQKCQYIVALVRECLRFYTVLRLALPRATVKDVIYEGELIPAGSTIYLNAWACNMDPEVWRDPEEFRPERWLEQPDAPLFTYGLGYRMCAGSLLANRELYLVFLRMLNSFELVQDSEIDVHPVRGSSDPTSLVTMCKHYKVIFKPRNEKALREALRAADERLAEHEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKQ8</accession>
    <name>A0A9W9CKQ8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translation initiation factor RLI1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP-binding cassette sub-family E member RLI1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">RLI1</name>
        <name evidence="8" type="ORF">N0V83_007097</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the multifactor complex (MFC) involved in translation initiation. Required for the binding of MFC to the 40S ribosome. Required for the processing and nuclear export of the 60S and 40S ribosomal subunits.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKQ8"/>
    <dbReference type="OrthoDB" id="6593433at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03236">
        <property type="entry name" value="ABC_RNaseL_inhibitor_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000144">
        <property type="entry name" value="ATP-binding cassette sub-family E member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000152">
        <property type="entry name" value="ATP-binding cassette, sub-family E, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013283">
        <property type="entry name" value="RLI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034348">
        <property type="entry name" value="RLI_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009945">
        <property type="entry name" value="PRK13409.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19248">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01868">
        <property type="entry name" value="ABCEFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="42"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="66"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="323"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00056499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00067238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00082546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4367514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="600" mass="67485" checksum="AC76B7C71A211DE1" modified="2023-11-08" version="1">MSDKLTRIAIVNSDKKCRQECKKSCPVVRTGKLCIEVDSSSKIAFISERLCIGCGICPKKCPFDAINIINLPTNLETQVTHRYSANSFKLHRLPMPRPGQVLGLVGTNGIGKSTALKILSGKLKPNLGRYDNPPDWEEILKYFRGSELQNYFTKVLEDNLKAVVKPQYVDQIPKAIKGTDRAVGQLIKSRSQMDNLDEMIDELELRHIFDREVQLLSGGELQRFAIALVCVQQADVYMFDEPSSFLDVKQRLAAARKIRSLLRPDDYVIVVEHDLSVLDYLSDFICVLYGKPAVYGVVTLPASVREGINIFLDGNIPTENLRFREESLQFRIAEAGDEYMIDHSRGYTYPAMEKTLGGFHLSIETGSFTDSEIIVMMGENGTGKTTFCKMLAGALQPDGTTKVPKMSISMKPQTITPKFTGTVRQLFFKKIKAAFLSPQFQTDVYKPLKMDDFIDQEVQNLSGGELQRVAIVLALGIPADIYLIDEPSAYLDSEQRIVASRVIKRFIMHSKKTAFVVEHDFIMATYLADRVIVFDGQPAVDARANAPESLLTGCNSFLKNLDVTFRRDPNSFRPRINKDKSQLDQEQKLSGNYFFLEDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKZ5</accession>
    <name>A0A9W9CKZ5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006426</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKZ5"/>
    <dbReference type="OrthoDB" id="3776608at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="156" mass="18128" checksum="8688195C011955C2" modified="2023-11-08" version="1">MPIYFDCNKVFGWTCESRPFSDAYCKDVRAKEDMQHQSDRRREQLERYYLAELENREMPKQEAVAAQVQQVHAPEKTEEPPPTEQLISTTLFKMSDDESDKRDSGVFLEVQESLIEDFSSGMNNVPAPWISLRQIADQHEFSLEMEGGTEYNPTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL34</accession>
    <name>A0A9W9CL34_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MoaB/Mog domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006412</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL34"/>
    <dbReference type="OrthoDB" id="448496at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047884">
        <property type="term" value="F:FAD diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042726">
        <property type="term" value="P:flavin-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00885">
        <property type="entry name" value="cinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47675">
        <property type="entry name" value="MOLYBDOPTERIN BINDING DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G11210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47675:SF1">
        <property type="entry name" value="MOLYBDOPTERIN BINDING DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G11210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="1">
        <location>
            <begin position="15"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="284" mass="31097" checksum="028166C517C91D05" modified="2023-11-08" version="1">MASSTQQKSLIHTAACLIIGDEVLGGKAYFAKFCFSLGINLRRIEVIADDEGEIVEAVRRMSKKYDFVVTSGGIGPTHDDITYQSIAKAFGLQLKLHDAAYERMKRLSKPHKSQPNFDWNVESPALTAKKRMVELPIDDSVSDEDQVIFVSDALWVPVSCVNGNVHILPGVPRLFEAMLDGLKSRILPRLTDPEGKGVLRILISTPMAESAVAGYLTELAAKAEPKGVKVGSYPRWGKDHNTVTLVGRDREFMEGLVPEVEAAVQGRRVAVEGEDDGDTSDKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL60</accession>
    <name>A0A9W9CL60_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_006472</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL60"/>
    <dbReference type="OrthoDB" id="8120565at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000026">
        <property type="entry name" value="MFS quinate transporter QutD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF14">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="359"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="492"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="64"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4368117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="559" mass="61257" checksum="79C6440AE285A8ED" modified="2023-11-08" version="1">MATDGTKGQLDRARTNEPSKDSADYYETIEPISTANLPTVLAENVSYGPNGIKGLMSSPTVFGAAFLASLGGFSFGYDQGVISIINVMPHFLDAFPPVKTGFGKGFMTGMLEFGAFLGCFFFPYLADKISRKRALTAMVVIFNIGAILQTSAVNYGMLVAGRTIGGIGVGTMAMGAPLYISEIAPPNIRGTLLVLESISIVSGVVISFWITYGTRNMVGEISFRLPLGLQMICATLLGIFIHFFPYSPRWLAMVDRQDDALSSLCKLRRLPPTDERIQTEYRGILAEVTFQNALQEKKHPGVRGIKREFQAWLDLFSRKTWRRLAVGCGVCFFQQFSGINAFIYYAPTLFQSLGQDAEMSLLMSGVFNILQLVAVAICFVVIDKLGRRPLAIWGAVGTTVSYVIISILAGLFGNDWPSHGAAGWACVAMAFMFILTYGVSYSPLGWALPPEVFPNAMRAKGVALATAVNWISNFTVGVVTPPMLEKAKYKTFVFFAVMCFLTVVWAVLLVPETKGRTLEEMDEMWGDSSAEEEKEILKEAVLRDRTRANMGMTQKTAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLM8</accession>
    <name>A0A9W9CLM8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_005829</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLM8"/>
    <dbReference type="OrthoDB" id="2399539at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052202">
        <property type="entry name" value="Yeast_MetPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782:SF12">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="242" mass="27488" checksum="3476E8AAAB3E74D1" modified="2023-11-08" version="1">MPLDLEDEYITKEGLSRQPRSLSSEPPTSITGAIHVVKLRRLWSKFADNLYPTTTKSSVSHSTSQHVLAGHLRQELEEWHAAIPETVDYSGSHPLSVFASKAWFQLAYDHSVLLLYRHYITLPPQPGEEESVEKAFEECAIRSRERWSQAATYRDIFETLSEKTISMICNNQSQEVHPGNDSADFGLDFTAAQSAVSQEWIMGLDDMPVPNEPGWFVQELLQGMRDYQQPDITFDDLGDFHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLN6</accession>
    <name>A0A9W9CLN6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLN6"/>
    <dbReference type="OrthoDB" id="415825at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37540">
        <property type="entry name" value="TRANSCRIPTION FACTOR (ACR-2), PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37540:SF5">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="665" mass="73893" checksum="0814CD237276342A" modified="2023-11-08" version="1">MGPNMNKVGSVSPKSKAVATSSAGESSELPPPNPQSNAKKKAKLKTRKAKRKASYGHPKFQFLTATDPSQFRDPEAERIVRSQAMIAFRRTGPSVVETLEPPEDASVAKGLAISGPSRAVVLPERLAHVAPDTAQARLLQARLDDQQYLYPSTSAAWWATNPSSGRVVDGEEIVFYCDEPRQRSRYDSAYSRSSLSASAKVVSYEDSDCHEDLQMRLLVGKLARFYQIGDGVDPFNVLPQFQSPGLNSLYLLRKCIRAFASDSTIVKWLPAMLSHPHLLLSSTVLASTWLDMHAGHSGDSVRTYLVKQETMDMINQRLHDPDTKLQDATLMVMLHLLAGTMWSCEEKTLKVHEEGVARFIAVRGGMQQFEHGVVGEIAAACCYHCDIFRDKGSLTIFYDWEPPTYSFDGTTAIPESPLFCPRTEFASFVNEARCSQSTFDLLCDMRDLTNLFVTSTGNSDVIPAIIVGEAVCLDPPADYAIKVNEIRKRLVSLPSAYTTGLPTSGDWIYEACRLTALIYTAAIIMLVPFSVAADPSRNQLLSGSRALRNLQASGHPRMVRLTESLYQVLERTDMSNLWDNMPGVLYWVCAVGAAAARTPTPITIPQQTKFRGEDYAVWIRRCLIMFSTRTMIILVFQHPLPVILAQKRLLKVQELIRIGARRFQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM29</accession>
    <name>A0A9W9CM29_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enoyl-CoA hydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_004954</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Mycotoxin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM29"/>
    <dbReference type="OrthoDB" id="2018133at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000001">
        <property type="entry name" value="Probable enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF54">
        <property type="entry name" value="ENOYL-COA HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0843">Virulence</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4370436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="145" mass="15423" checksum="BFDB4580F60FF35A" modified="2023-11-08" version="1">MQCDMIFASGDAQFGFPEVKLGTIPGLGGTQRLTKTVGKQKAMEMILTGTPTTASDMERLGVVNRVVASEQDVFEEALKVAQSIASFSAPTIGLAKQAVVAAEATTLEAGLEIERALYYSSFSLADCSEGIAAFLEKRPASFQHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMT0</accession>
    <name>A0A9W9CMT0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7907 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004767</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMT0"/>
    <dbReference type="OrthoDB" id="3515453at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057229">
        <property type="entry name" value="DUF7907"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25484">
        <property type="entry name" value="DUF7907"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7907" evidence="1">
        <location>
            <begin position="24"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="206" mass="21700" checksum="C53D4FE75C9EAC8A" modified="2023-11-08" version="1">MKKTTSLVASLIATASAQYYNITSPPFHLTVTSADGTTNSTLSACHTGAALESLCLSTGPPTTSKPDPIAAALFAFNTSIYSQAPAPGLGAPGVLTYSLPSIPPIPSTLFFSYDPTTDIALPILGPGDENAQQLAFADDGRLSVQGYVDWSVSPPNGTGGVREYFRWYACVTYYSGYQYEALAWGLGPGKPENPSCVSVDVRRVFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMU1</accession>
    <name>A0A9W9CMU1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_004797</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Component of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17919">
        <property type="entry name" value="DEXHc_Snf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15489">
        <property type="entry name" value="PHD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056616">
        <property type="entry name" value="Chromo_MIT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055565">
        <property type="entry name" value="DUF7141"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040934">
        <property type="entry name" value="Znf-CCCH_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041684">
        <property type="entry name" value="Znf-PHD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF17">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23615">
        <property type="entry name" value="Chromo_MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23614">
        <property type="entry name" value="DUF7141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18585">
        <property type="entry name" value="zf-CCCH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15446">
        <property type="entry name" value="zf-PHD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="747"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="1052"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="175"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1355"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1433"/>
            <end position="1494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1612"/>
            <end position="1714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="185"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="258"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="286"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="297"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1368"/>
            <end position="1382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1442"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1469"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1639"/>
            <end position="1652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1685"/>
            <end position="1696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4371578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1714" mass="193452" checksum="0129652C3F23C54E" modified="2023-11-08" version="1">MSSDEDGSELMAAFKKKDDRKLITACPKSPEPAAFSPGNFISDDGSRGEEYVTTVASLPKSRRAVCVRPLPIQNKHDYIYYEPQDEVQSILREFTRKGEIMCQVRLWNHRSKVISFDKLLELPGGPEALRVFNTNDDENATTSSADEATMRLRGKTSKARNDDFIDISTIVLSSSEDELTPASRKLLKGKGRTLAKRRADSGRSSRTQSRGSKLIELSTSEDDDDSDNGGRKRRRRRKDVQEPTRRSTRQPMPVRAYDEEEEDEEEDEETDDSSASDILRSDVLPGRKRKRRTLRTLKPEKIPRTGVRQSDRSTRATNNMQETEMDDIYRSDSTPKVSAQKMTVVREVFTTLPRSDLFRSRHAEGCEVCFNGPNIAPLIYCQGCSLAYHKNCLGNRSTRDHLVTKVGNENFMLQCKRCINFYKKKDATAPDLSKCHDCIQVGASCKPFRHRKTTQQEMKDREENGGQDPIVAVPSELINNAHNVLFRCTKCSRAWHYHHLPPISQYAMDVNRDDEEIADERFREYSGKWLCKECDDTQDQKVGSIIAWRPSDVETYRPGTSCDMVSEDDKQYLIKWEKQSYFRAAWYSGAWTWGVTASAMRKAFFKREEGPKMRTEDAIPEEYLRIDIVLDIKYTSYVEVRSEEIDKARIKEVDKALIKYKGLGYEDAVWEKVPTSEDGDRWLDFVTAYNDWVAGRYIRYPKQGPLKARLEKARGTPFAKLERQQQPENLVGGELMKYQVEGLNWLYYQWYCQKNGILADEMGLGKTIQVIGFMATLIQEHNCFPFLIVVPNSTCANWRREIKQWAPSLRVVAYFGSSQAREMAYKYEMFPQGTKELRCHIVVTSYEAAADDSCRRFFRSVSWGGLIVDEGQRLKNDKSQLYTALTSVRSPFRLLLTGTPLQNNARELFNLLHFLDDTVNAAELEEKYAEMTSENIKELHDQIRPYILRRTKVQVLTFLPPLGQVILPISMSHLQKQLYKSILSKRPELLKALFTSDKTLKQQERANLSNILMQLRKCLCHPFVYSREIEERTDVAAVSHRNLVEASAKLSLLELLLPKLKERGHRVLIFSQFLDMLNIIEDFMDGMQLAYQRLDGTMGSLEKQKRIDQFNAPDSPLFAFLLSTRAGGVGINLATADTVIILDPDWNPHQDIQAIARAHRIGQKKKVLCLQLTTRASVEEKIMQMGRKKMALDHVVVEQLDTDDIEDQDVESILKFGAAELFKDDNADHDIRYDDASIDKLLDRSQIENTKTSDDTSAESQFSFARVWANDKGGLQDDFDTVDQEVAPDPGVWDKILKERQAAAAAEALARAEAMGRGRRAKANIDYATEKDGVETASVAGEDIFEDILPSPIKKADKRRKKHTGSESDTDFQADTEEESEPEPPIADIDAARELEGARRRSTTTIANSTLYKQNGAGASKVSSTSTFRFPSVTLPLPNSAQQGPQQPPRGGVKIRLPPVAASKESTHQAPPKSTTSSTPSAQAALQAPKTNGSAVKPFQRANIPIRSPLEPTYGSFSNRICPACNKNHPQGACELKAAGVEHCGLCGLAHYGHSRTCPHIKSETQVREMLEALKNSPEKKELVDAALKYLRGVKGTLVQQKKKDREKAELLKNGGQLPAPAIARPPKNTTPNQMQRPPYAPQNVPPQPPSSHPATLYRSGVPNAYSPYTPPVNPATGSVGSHLQQQQRAMQMQAQGGYEDQEVESALRGFLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMU9</accession>
    <name>A0A9W9CMU9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004361</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMU9"/>
    <dbReference type="OrthoDB" id="2533647at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13577">
        <property type="entry name" value="SnoaL_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="28"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="250" mass="28442" checksum="0672EC248E0F9644" modified="2023-11-08" version="1">MATDLHNLPAGSRPDSAIRNNGPDHLVLERLKLRELAEGWPCYRDACEWQNFASLFHPGAHVYTTWSGRIPYTDFIAASKAGMDKGAFIMHRCHGCTTDINASGTRAVTKLKATITQRFVIPSSSSSSPSSGEEECEFDVEADCRFIFFFEKVDDKWGARLVRHWYEKDKIIPANPQRLPPVDEAKAKTYPPGYRYLAYWQEKTMAGVKVKMDMPGHRRDVAWKLNLEGHDRLYWQAKSWLEDGTGIDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNJ8</accession>
    <name>A0A9W9CNJ8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003507</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNJ8"/>
    <dbReference type="OrthoDB" id="5386330at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053157">
        <property type="entry name" value="Sterol_Uptake_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784:SF5">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="430" mass="47700" checksum="B0A186E1ABB31D22" modified="2023-11-08" version="1">MCGHFDPAPKPSTLSSAFATEKVSSHVLIFPQKPDNTPTPPADHGLLDPSVQAALLKDFPAADQLRFLQILKHFATDTIPTMLSLEPAQAAWRKAVPILATNYKFLTHGILAVASLHLAQLAMTQEERQTYEDIAANQMNVGLTQYRVEVQDVTTQNAEALFAFSTTITTFVLHSAGAECKAILKLMKENDWSIEDRMESISALSNAVFRVFRSVRGVLVIIVPCWHHIVKGIFRPVIARDWWPPGVPTTPEEHDEDRKLQHLNTMWSRPGRTYDYSCDTLRHALKDLKEAFALVSRVMALAQSGDTVCASTFDWTVVFHWPVQLSLDFISLLEQRRMEAWVLIAHFAILLARATGVLWLDGLGTSLLATAALVIGEDNWNWIAWPAAAVNVDLASLRNVAITSSKTDTPSRGTPTERPPVSFSTESSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP86</accession>
    <name>A0A9W9CP86_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_003238</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3182339at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022099">
        <property type="entry name" value="DUF3638"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022105">
        <property type="entry name" value="DUF3645"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046541">
        <property type="entry name" value="DUF6606"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051346">
        <property type="entry name" value="OTU_Deubiquitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367:SF32">
        <property type="entry name" value="DUF6606 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367">
        <property type="entry name" value="UBIQUITIN THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12340">
        <property type="entry name" value="DUF3638"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12359">
        <property type="entry name" value="DUF3645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20255">
        <property type="entry name" value="DUF6606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DUF6606" evidence="10">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3638" evidence="8">
        <location>
            <begin position="1948"/>
            <end position="2171"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3645" evidence="9">
        <location>
            <begin position="2291"/>
            <end position="2323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="3087"/>
            <end position="3121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="3087"/>
            <end position="3101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="3102"/>
            <end position="3111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4372947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="3121" mass="356425" checksum="81F264D05167DEC9" modified="2023-11-08" version="1">MLIIHASAQNCALLVYEHSSDSGERRVIFEAFEASATCDMVLATENALLWDFPGCAVSVSSETFAEHSFQESLAAFVEQASSELVTKFATVTCKAAATLPEIRDTADPTLVSGLLMTILEANGHVVHVPVLRKRVHDTVCFDRARKPWRRSPFYLVVRVGVQRYLYKLLGPEIGRLYYKCIMGKLLSQLLEEGLKQIPFEATLHLRQKLGRRLAKLDSDTDRASEPAKKAYDHLLRSLESDFDGILSTTGGYLKSVWRNHRTRHERRIPLLRRQAEDSELHLRLANSGKLLHAIMFHADNITVESFAPSELLEQYERSSPLVKPYMRIARHHIALSQHHEDVIFPAKSSTYPEGSQFIMDISKVIEDYVRKAKALSDGYPDQLSQMLLHLLEMWVLMDKATVQLYPLLKEYHSGFNYDILDPLQLLTLEDLTRVQNVQAYLANRNPIQQDLRRGTIFDDPSDDCFAARYYDDYDVSGDLSEIRESIEEDAAIAYEAKETEWKKTTQRYKDLIDQRDKTYCIFDPVPLWDGTTDSRHRWPCDWHALQDEARNLMIKIYEHPLPSWEPAAKATLFELRCPEDFAAYRDVTRRIISAICLPPAISFNKVSLMRKYTKLRLYTNDTECQVVLASEEKAHLKTHYSGWDFPIDLCTVRRACGLKPRYYDTSTQTWTEDWNKASLWHHFPLRLTNGSQFQVLRLSYGGWPSSNQIQASQTDCPTDVNVHEFMAWQGLMVGTHCRWLGLLRELGSTNINFSSEATWILVHRLCTEVGPSTVGKDPLRDVHSAILDVTFCMKLLHQIQQRLNAIDRNWREPVQMHILVTILSKVASLSSAVHSEAMDLLQQARLVTDGWRAELQSTVTEDSKVTQFAIWASLLCRSTLHLNRNLLLDAKALRHFVDASISLQYNLTEMDSMPCSLRNAIIQDTLFAYENREFIKHAILAQPSALVDAVHELWHIPDYQKATNTIPQPIDQKTWLLLSFGSEKSNHSYSVHYNYVYGTLLVDGKEMGTLPLEYRSNATFRATFGCMNPIVFPSPLHGMSFTISECMKNGHRIHLGFRSNATGGKDLVIRAVQDGQILELVSRRVFGSQGFSDLPAPLVEGCFHWLNVRTGRLEIRKQDIWVSKQGNWWIHGIQTGSHRAFRHYREGKETRLLDASSAMAQDVFRIFSYFEQPSHIVVFTSRDGVTVELKRLELSFFVSNNGLLHSKRLAAVIPQNQDAGTWYGLKSKIIIQSVANLRQKSILVPAGRLKVSKEAIHVSVYIETGGDVYLQFGINEVLGRVECPPEPKLLYTKALFHAYTSHVLCDPLTRRTGSEEAIYLLRTGSYMPWSPLAESQVDILRLISDLTPSREYYPKDKQCMETITWQHNIPISMQDDRYRALIERILRRSSDLSNFFLDQPSQSRIDIPPGNPHLESRAMLPRHTRRTKEDSVYHARDSRRATIDRRNVLEVSELLCKWRLPVFDGSSLITLLHDAPVVGGYDKLFQKCLLTEHLAIDLRAEWGALARKLLDSSDEDRFRLMFLLASMGFKEDASMGLIRMLIAFAMIPGIAMLEAPQYPVYYHFRADGTPQEQNLLPWIEKAKVPFSSTGFKKRHAMIVAQTIFEKNVRQSCKILVDSILEQWPNPEIDREKLVSVDATYLDIDRALVNVSAEWVRLTQNHELALHLGKVQEVVDRVSTNRESMPDQKYPPSHNNEVVTQMGIMIYPMRARDRDDSSLSKLLRNTVHMHAIPDRSLLSPSSATFNGTRLAIRPGNVPRNLHNRYDVLSGATDHGWPSQSANVRKIPIKRQVPEIKLLQSLAARFEGTSSFVQSRYAKELKASINALQGYIRRREEATHRSRPRITKVEIDPSKETAMNVAKQVRESLSEHDPQARWLQKVDLWPKMTTIDLLSELRSTSGTTFGADTREALVTVGLAISKWQHLLRIQDAQKRCRQQQERDELANQGHTNWNPLSYPDWLLLEIDGDVLLREEQVQVALATISPESGENSVLQLLMGKGKTSCILPMVAAVLANKSDLARIVVPRPLLLQSAQVMQAKLGGLVNREIMHIPFSRKTPTNRTLIRLHRKLHERVKNNSGIILALPEHILSFKLSGMQQLCDNNLDEASTMIRTQHWLNEHARDVLDECDVSFAIRTQLIYPSGSQMTVDGHPSRWQVTEILLNHVKDFLPAIQSRYRRSIEIVGRGTDSFPLVYFLRKDAEDHLIELLVRLICTGQAHSILPCVEFPDRLQQDVEAYISRPTLSQEVARRVIEYFWDKQKLLKTVNLLRGLFVHRILISTLKKRWNVQYGLHPTRAPIAVPYLAKGVPSPTAEWGHPDVAIVLTCLSFYYQGLTLSQFKQAFEHLARSDEPSVEFEKWILNAELPHGLDDFAALNVDDHWQIMELHRFVRHNAALVNFYLNNFVFPKYAKTFSLKLQASGWSLCPSISVHEKGCRVSGFSGTNDSRHQLPMLVKQGDLHHLAHTNAEVPFYLLAPRNRGYERMIHPTGARWNEIDLLDNLSNPSRRPPYELNFHIKSKIRILIDAGAQVLEHSNRDFAAAWLERDYDAAAAVYFDDDHRAWVLYRNSNKRTPLLASPFADSLDRCLVYLDESHCRGTDLKLPADAKAALTLGPHLTKDALVQAAMRLRLLGQTQSVIFYSPPEVHQGILDRLRKDDFFRPNTADVLQWVIGQTCDAIEQQEPSYFAQTTHYLNQEQAQLDHPEYLQDNVQRNAFLAAVRVKESMLLKKLYEPKTQRTTAKSGTWDPRLQRFADALHLRKKHFQDRGSAVHASILEEVEIEQEREAEREVENEVEDVRETQRPPRFNAYNTRNLHEDVKHFATNGRLVAGSDAYKPMFSVMKGTALGAKHALSMSMNSNLWVSKQFTRTVEAPHGSNDNFLRPCQWVVWSSISQKALVVSPEEANELTPILRSSTSHEVDGDGSVHLIVYAAPVTRRMLHFNRLDYYAVPSLPSDFKAPTWLRIELGLLSGRLHLEWDEYYELLGYLGLSQDLSLDSQKQAFAQKPLTFLHEWIALRRKGQDFEQTPMGFVTTGKPLTANHAFFRAFTHECDSDIRPQVARLTSGRAVEGGEEEDYGDHDDEEFVPAVEHIDDSDVEDEKLGSEDEGEYEDATESPKSAGDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPF8</accession>
    <name>A0A9W9CPF8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Suppressor of anucleate metulae protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPF8"/>
    <dbReference type="OrthoDB" id="3785113at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4373306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="471" mass="53425" checksum="09832CDE5D692427" modified="2023-11-08" version="1">MSPGRCNFCNRPGTLYCNDCATYDANYNIIDAHWYCSGLCKAADEPKHLGECRAHLLMPEELFRRAEQAGEAAQSLFYAFVQNTWAYDMSTIRIVRDQDDEVVAVEVTAGAGVHAGPGAESTCGRHGGGWLVNFPDAAFDIYDQEAKRAVLADQNSIWAFVSMHVAVEALFKDLVNDVGKDIKEVVHYVPHDTRRLVEHKGRFGLARAERENVVYPDYDDQGDLKGVYLITLKDGTQIALDLAGAQYGLSHTTILPWSTYLNRHVGKSIKYRAPFRSHYTKHADKMSNYRCITPLTVIMEQNRHFNKFVANTKEDWNLDLKDLPTMSPQDFDDILHHLVEMAHVTLLHLPRGLDDGGPPEEAFDLRHPKVVASLPRRRAPSSSTTDVSSLVSLPLDLNFMSPFSWYSLRRLIQMPASEVKYHEKKTARKLLSMRCAYKLPGDWRVVFLQDTLPSVNVPRWCVSENPGWKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPH0</accession>
    <name>A0A9W9CPH0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-dependent RNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_003630</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RdRP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.48" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPH0"/>
    <dbReference type="OrthoDB" id="10055769at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031380">
        <property type="term" value="C:nuclear RNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007855">
        <property type="entry name" value="RDRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057596">
        <property type="entry name" value="RDRP_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079">
        <property type="entry name" value="RNA-DEPENDENT RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079:SF55">
        <property type="entry name" value="RNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05183">
        <property type="entry name" value="RdRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0696">RNA-directed RNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RDRP core" evidence="3">
        <location>
            <begin position="378"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="184"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1224"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="141"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="207"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1224"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1236"/>
            <end position="1246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4373336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1246" mass="140715" checksum="BD9587F3B3F6AAB7" modified="2023-11-08" version="1">MNSSAQRRVQASLFASSRSASKKRPSDSSDESRYSPKLSKTAQGKQAVYFAKPEAITTPPLFKKPSREMAPSFQTAGSGKTSANTSFNESATSSQNTNATTNTSFTSYDGSTDVRDSKMTRTSSLTIGSLDDDDMLNATSHSEREAFGWDKQESSSQNRSTFGSIDEDGLQDVLLQLEGETKPISSPVAKRGPVVPVESGIPRNHRLGSSMSVTASFPSDGASTATISSNKSLLRPRSSSVDMPPPKKPQGPVSPVVSPVKEAHYIRGLPDQNLFVDEAPQALKGVPYFILFICQRLVAAAYISLQDLTKHMDLAAACSSSEAFWTSIRNHPNPKIALQKPQEHSRIWQAARRRRFDGYTFKGRIDFNLKSSGQVFNLSLQPVHAEDSCRFQRKFDSDRFLYLTAPVFDYKKTNRFDELRMKQIELRWKEWLQKEHVFLGRKWSVVHVEPTKRDKGKRKETTHDKRLVLFATKGWGIDTPCDIGSMLNWFLPFQKNQGQSFCKAYARLDLGLSRTVPTLVFQPSQIIRVRDKLANGERENSIFNDEKLHFESIPEDQVMNDGSSVMSVGAAQKIWGLYLEATGLNSRTPLPSAFQGRIAGAKGLWMISGETFSRDRNDTDIWIQISDSQLKFEPHDEDLSDETYDPIRLTFEVSNFSSGPSPSDLHISFIPIMIDRGVSKQIVADFMIDCLDADRSQLLDVLTDSARTYDWLYRNGSKSSNANSEISWQAGLPVSAEEKVKLLLESGFTATKFSYLAKSIERFITTQQIFQESKLRTPLGKSTYLFGVADPLGVLKPGQIHVQFSASFVVDMTKEAFLNLRNMDVLVARQPACRRSDIQKVHAVVCPELSHLVDVVVFPSRGQYPLAGKLQGGDYDGDIFWLCWESRLVDPFKNAPAPMQSLDPMPYGIKVNRQKVKDVMNPTDLTEVGKLLQKAFEFRSAEWLLGRVTVFLEKQAYRENQVYSTTLDSLCGLHDLLVDAPKQGYTFTSKDFDHYIKDILSISELPKVPAYKAAMEDCLYVKELGEIDKVREKKHRHKPNRVLDYLYFGVVRAHNVETLEQVKRVFSTATTIDETLLHPYKHLEARQHPIINEELRLLKDKLAQLSYRWGSNFKTGAGATPEQKNVLVDECHREFLALQPTKTNHPEVKAWLEPYTGPSSFSWDVIRASTLYARFPYPEKMNFVFKMAGRELVGLKARSIPNSTHIVPFIRGIMRPKRIKAPIEYEEDEDEESEDEFKKAMEQITE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPV2</accession>
    <name>A0A9W9CPV2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_002796</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPV2"/>
    <dbReference type="OrthoDB" id="1393670at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:001190">
        <property type="entry name" value="Short-chain dehydrogenase/reductase SDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051122">
        <property type="entry name" value="SDR_DHRS6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43477">
        <property type="entry name" value="DIHYDROANTICAPSIN 7-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43477:SF1">
        <property type="entry name" value="DIHYDROANTICAPSIN 7-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4374057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="285" mass="29478" checksum="6B02E9C223A9960F" modified="2023-11-08" version="1">MAEIPSTPPSRGLKGKVAIVTGAGCEGEGIGNGRAISIILADEGCNVVCLDLNLEWAQKTAEIAMSKPGRGKAIASQSDVTKAEDCEAAVNLALKEFGRLDILVNNVGIGGAAGTAVEVDMAAWAKGLEINVSSMVQMAKYSVPAMQINKYEEEMKGVIVNMGSVAGLKGGTPHLLYPTSKGAVVNMTRAMAAHHAEQGIRVNCVCPGMLYTPMMYAKGMSDEAREARRKRSLLGTEGNGWDAACAVAFLASDHARWITGAILPVDAGATAAVGIGMTKAASVNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPW9</accession>
    <name>A0A9W9CPW9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Long-chain fatty acid transporter fat1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">FAT1_1</name>
        <name evidence="7" type="ORF">N0V83_002723</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPW9"/>
    <dbReference type="OrthoDB" id="10253869at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044539">
        <property type="term" value="P:long-chain fatty acid import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000002">
        <property type="entry name" value="Long-chain fatty acid transport protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107:SF15">
        <property type="entry name" value="FATTY ACID TRANSPORT PROTEIN 3, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="60"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="6">
        <location>
            <begin position="548"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4373984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="691" mass="76600" checksum="FCB9128B052097AA" modified="2023-11-08" version="1">MSALQLPYTVPAAAAALAYLNARHGLTYDWPLLSSFISASISGALHERRDHLNLFYELEAHAQSSRANHAWIIFQGRTWTYKEAYDIVLRYGTWLKSKGVEKDEIVAMDFVNSETFIWVWFGLWSIGAKPAFINYNLTGKPLVHTIKTSTSRLVLVDQEGKGKFEEHAMKEHGFELVKEESREGQVKFQFAMDQSEVPTSVKKQTTTPQAAVDAGAVSSEPLQRQLDIVFFDDNLTSHILTLPPTRLPDSARANQKRNSMAMLIYTSGTTGLPKPAVLSWGKGSMAAKFVSSWLSLKPEILYTCMPLYHSSASLLGVCAVLRAGGTIVLSKRFSHKTFWPEIRASNATIIHYVGETCRYLLSAPPSALDTQHKVRAAFGNGLRPDVWEPFKSRFGIRTIYEFYAATEAPGGMWNRSSNLFSSGAIARNGTIASLLFAPAMCVVRINHESSSPEPLRSPTTSLCEICLPNEPGELLYALDPADTQQKFQGYFNNPNATHAKIIRNVRKQGDAYFRSGDLVRWDSEGRWWFVDRLGDTFRWKAENVATSEVAEVMGRHGDVMEANVYGVAVPRHDGRAGCAAVVLKASAAATTTEGEGEGRKLPLPSEGILTSLAQHVKKELPSFAVPIWLRVTSEMQTTGTNKQQKHGLQQDGIDPAVVEGKGDVLYWMKEGRYVRFTKRDLEGIEGGGVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPZ6</accession>
    <name>A0A9W9CPZ6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">SecE/sec61-gamma protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_002918</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPZ6"/>
    <dbReference type="OrthoDB" id="2401875at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12309">
        <property type="entry name" value="SEC61 GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01067">
        <property type="entry name" value="SECE_SEC61G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4374177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="69" mass="7669" checksum="DEB22D134DAA90F7" modified="2023-11-08" version="1">MENVKEFADMPAEFLKEGTLFMNRCTKPDKKEFIRISQAVGIGFLIMGVIGYIVKLVHIPVNNILVGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ60</accession>
    <name>A0A9W9CQ60_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">GTP-binding protein of the rab/ypt</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">YPT52</name>
        <name evidence="12" type="ORF">N0V83_003113</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ60"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016050">
        <property type="term" value="P:vesicle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000464">
        <property type="entry name" value="GTP-binding protein ypt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4374372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="220" mass="24162" checksum="A5C64BCC4E5CA646" modified="2023-11-08" version="1">MAARAPQGQRPGGSRFAQFKLVLLGESAVGKSSLVLRFVKDQFDDYRESTIGAAFLTQTIALDENTTVKFEIWDTAGQERYKSLAPMYYRNANCAVVVYDITQAASLDKAKAWVKELQRQANENIIIALAGNKLDLVTESPDKRAISTADAEQYAREAGLLFFETSAKTSENVRELFTAIAKKLPLDQAGPRNLRAGQQRQGVNLRPEANQTQGPGGCNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQA4</accession>
    <name>A0A9W9CQA4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tripeptidyl-peptidase II</fullName>
            <ecNumber evidence="4">3.4.14.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">N0V83_003115</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Secreted tripeptidyl-peptidase which degrades proteins at acidic pHs and is involved in virulence.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal tripeptide from a polypeptide.</text>
            <dbReference type="EC" id="3.4.14.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="15">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
            <location evidence="3">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.10" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQA4"/>
    <dbReference type="OrthoDB" id="409122at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04056">
        <property type="entry name" value="Peptidases_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11377">
        <property type="entry name" value="Pro-peptidase_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000015">
        <property type="entry name" value="Tripeptidyl peptidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015366">
        <property type="entry name" value="S53_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030400">
        <property type="entry name" value="Sedolisin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050819">
        <property type="entry name" value="Tripeptidyl-peptidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218:SF39">
        <property type="entry name" value="PEPTIDASE S53 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218">
        <property type="entry name" value="PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09286">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00944">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51695">
        <property type="entry name" value="SEDOLISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 15" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8 16" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0843">Virulence</keyword>
    <keyword evidence="13" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040866727" description="tripeptidyl-peptidase II" evidence="16">
        <location>
            <begin position="19"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S53" evidence="17">
        <location>
            <begin position="215"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="15">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="15">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="15">
        <location>
            <position position="527"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="569"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="570"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="588"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="590"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4374374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="613" mass="67607" checksum="FB7F481B10B9A1F2" modified="2023-11-08" version="1">MQLIVLLAAAIAAPAVLATPIARSPYVVKETHFAPREWTKLDRANGGKTIQLQIGLKQGRFEELDRHLHEVSDPDHARYGQHLSADEVDELVAPSSETHSLVLDWLRESGIDTDRLGYSSAKDWIIVHLPIEMVEDLLDTEYHNYKHKDGSVVARTTKWSLPRHLHSHIDAIQPTTSFFHIAAKEATYVSEDAEVPASYQHPKNESIASVCNVTSVTPECFQTLYSTKWYKTQASDKNSVGFANYLGEIPIRPDTKLFLQKYRPEAVSQAYAFKQLSIANGPVQDGPLTYNQSSVEGISREANLDVQAIAGISWKTPITSFSTGGEPPFNPDTSTPDNTNEPYLVWVNWLLQQRAIPKVISTSYGDSEQTVPRSYAERVCKQFAQVGARGTTLFFSSGDRGLGGTNTCYSNDGKNTYKFLPAFPASCPYVTTVGATMNFEPEESAYRPASNTSAGFRDLYSSGSGFSNYFDRPAYQDDVVPDYVDSLGDQYEGLYNPEGRAYPDLAAQGLYFAYFWNLTEGVISGTSASTPLTAGIFSLVNDALIASGRPTLGFLNPWLYKKGYKGLTDITKGFSYGCNVQGFPVTEGWDPVTGFGTPDFPKLLRLAGAKGHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQW9</accession>
    <name>A0A9W9CQW9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ornithine decarboxylase antizyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_001066</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ornithine decarboxylase (ODC) antizyme protein that negatively regulates ODC activity and intracellular polyamine biosynthesis in response to increased intracellular polyamine levels. Binds to ODC monomers, inhibiting the assembly of the functional ODC homodimer, and targets the monomers for ubiquitin-independent proteolytic destruction by the 26S proteasome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with ODC and thereby sterically blocks ODC homodimerization.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ODC antizyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQW9"/>
    <dbReference type="OrthoDB" id="5959761at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008073">
        <property type="term" value="F:ornithine decarboxylase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075523">
        <property type="term" value="P:viral translational frameshifting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002993">
        <property type="entry name" value="ODC_AZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038581">
        <property type="entry name" value="ODC_AZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10279">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE ANTIZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10279:SF10">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE ANTIZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02100">
        <property type="entry name" value="ODC_AZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0688">Ribosomal frameshifting</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4375789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="154" mass="17466" checksum="BA10FE03C91B8FAD" modified="2023-11-08" version="1">MTSDMVNDYGVDARRYSDSASSMDSSVSFPAPIQSLEKRAITDWIEMWDYVGGIRFRGFVADKEGEKAMLVFFDQSVIEGDLKAGLMALLELCEVDYFSCDRLVVCIDRHADQVAKDTLTKDLGWIGFSLTTLDEFSHGEELISDKWLFMEMET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRG2</accession>
    <name>A0A9W9CRG2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_000805</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRG2"/>
    <dbReference type="OrthoDB" id="2152029at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF8">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="136"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4377975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="383" mass="42822" checksum="637D02381288A4A6" modified="2023-11-08" version="1">MFSTPSRAMAIFAYQPFKAIYVFVALGFELARLPVFLVKYLVSYGRQHPEWTFRQAITVRIFYSAVYHISQIQLKTPLPLAPGPEKERFVSCKPAQDRYYKGPLRNNTEIKPVEVGATWYPAPLSAASEKINIRVILHIHGGAFVTGDGRTGASGYMARKLLKHSTATHVFCPQYRLSTLPASKTSNPFPAALQDSLTSYLYLINDLKIAPGDIILSGDSAGGNLSISLLRYIVEYGSDLGLSNPSACLLWSPWTNPADITASYVHDNDHFATDYLSPTFTHWGSEAYAGLSGLQSLDQPYVSHKNRPFRTKVPLWVNTGGGEVLYYDDKEWFNMMKEADNDVTLDVEKHVPHDVLLIGNMLGFDKEATDCAKRAGEWLRTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRP7</accession>
    <name>A0A9W9CRP7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H/ACA ribonucleoprotein complex subunit NOP10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Nucleolar protein 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Nucleolar protein family A member 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">snoRNP protein NOP10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NOP10</name>
        <name evidence="9" type="ORF">N0V83_000126</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NOP10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRP7"/>
    <dbReference type="OrthoDB" id="13807at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904874">
        <property type="term" value="P:positive regulation of telomerase RNA localization to Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.40">
        <property type="entry name" value="H/ACA ribonucleoprotein complex, subunit Nop10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007264">
        <property type="entry name" value="H/ACA_rnp_Nop10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036756">
        <property type="entry name" value="H/ACA_rnp_Nop10_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13305:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13305">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NOP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04135">
        <property type="entry name" value="Nop10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144210">
        <property type="entry name" value="Nop10-like SnoRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4377302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="68" mass="7942" checksum="E7CE981B2066E9D5" modified="2023-11-08" version="1">MHLMYIPDPANPTKRIYTLKKVIDGEVSKSAHPARFSPDDKYSRHRVTIKKRYGLMLTQQKNKVAERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRS8</accession>
    <name>A0A9W9CRS8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Septin spn4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">spn4</name>
        <name evidence="6" type="ORF">N0V83_000098</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRS8"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032156">
        <property type="term" value="C:septin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000238">
        <property type="entry name" value="Cell division control 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="5">
        <location>
            <begin position="24"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="314"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4377274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="381" mass="44225" checksum="BEFF63377BC99F5B" modified="2023-11-08" version="1">MAESASPIGIANLPNQRHKIVAKRGAAFTIMVAGESGLGKTTFINTLFSTTIKNYADHKRRHHKQMDKTVEIEITKAELEEKFFKVRLTVIDTPGFGDYVNNRDSWQPIIEFLDDQHESYMLQEQQPRRTDKIDLRVHACLYFIRPTGHTLKPLDIEVMKKLCTRVNLIPVVAKADTLSPADLNRFKLRVRQVIDAQGIKIYTPPLEDDDAAAMQHARGLIDAMPFSVIGSEKDVQTNDNRTVKGRQYAWGVAEVENEEHCDFKKLRAILIRTHMLDLIHTTEENHYEAYRAQQMETRKFGEARPRKLDNPKFKEEEEALRKRFTEQVKVEEQRFRQWEQKLIAERDRLNKDLEASHAEIKRLETEVDSLQGSVNRSHGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXT2</accession>
    <name>A0A9W8XXT2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_010426</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXT2"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF76">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="307"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="120"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="191"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4362333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="627" mass="69624" checksum="CF7C6F98FB5865BF" modified="2023-11-08" version="1">MASNSSISSHNFQKLKLDTSAPQKPVASAFATYPRKESSQNNHGQSQLTAELAGAESLPDGAYIQRNQNDRNGNGHNGSYDGSVASSRSEEEEEDIELRFKKRTMSISFNHEVSLEDGNRLGLEVPVPKPIRQSHSRTPESGSYSDQEYLEMPYSMRGHLQHRVGESRYPLLQTTVDDLAKNPEYNDQVASLTSETTASPPLEEAQTPLETPSEYMLSPLPATSPIEFPLFSPRRTAPPRTKSYRSETGEDGSLRKASRRSSGRSGRSLSSMSPAASFLARYKASEAPIKPSEPDDEGQGIGYDSEFIIGKQIGFGGFSIVKEVTSMENGKPVVNAVKIVRKLQMEKSELENEQIQTQFDHEVDIWRFLRHPYILPLLRVYTTDFATFCITKLNKGGTLFDLVRDTRRKKKAGLPEHVVKRYAYQMASAMRYLHNDVQVVHRDIKLENVLLDMTVPNAEQDGGDILLCDFGMADFIISDQRDGPEPHSIGVNQNIGPADTSTSVAGSLQYAAPELFNAPGPVFSPAADIWAFGVVLFALLTATLPFNDGLDIKTTERIQKGNWSEDLIRSAEAVQGGGVEDALALVRGCLELDVEKRWTVHDILDCAWLRDCEERYENVSRAWLSDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYD8</accession>
    <name>A0A9W8XYD8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_010459</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5430750at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494:SF1">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1084"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1118"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="88"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="759"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1165"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1244"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1326"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="170"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="771"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1185"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1251"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1347"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1361"/>
            <end position="1371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1396"/>
            <end position="1413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4362366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1413" mass="159215" checksum="4C7AC5D13D69C5B5" modified="2023-11-08" version="1">MWAIRPLLEEVYTLLNQQELVDDIKLGRYEIQTDDGATIIPSVWSAIVKPSSKIQIDFRSDRPIGRRRSTQDAESVIETRDVRVTINADSPEFALSNSEVSDSNGDESEDDSESQSSTESEDEASEQAVLPEPVRDAAPPLDAEGNKLSFSVDTSCAKAIQRPKDPKINGVNSKVPSSQKDASDTYVETLRITRATSTVVESRSLIQIHTLPSTDNIHLRESASMTWFHIPATRLDYSRFRDVCLAVPHLSDRLYTLIWETFKAIEKDKIKAFLDGMFIEPGTVIRADEKGQAESQSVIFSCIPYFDLQPPAKKLATGQGDRLFPSRTLMQSYYPYEPVRDRDAEQAYRKFGTDRSGSVVNVPNMWTMNIGTDIVVTCGHKPLSQELIRSMEVIQEDLAHVGTKDITNNPLTRLRLRDWDGRVLLFPLNACRSYFQMEQRLRELNWTVRGRMDRRRLRLVQRTSDGEKNVAVKGWMDIIRQTDLIFIDLGVVDDAKAKEPQPEPPEEDISNLLTPSSTTLVPPFFLWPSASLGGMSNDMEAAPESTPSRVKRSMYCLEHAEKGMRSETLDVYDTVNEVDKTFTSTSYYRSLPELTHSDVLAAFSSLRHRPVQVEGAASSSHTHHQAIISTQCTGIVERSSKFFKIVYATFKLFVEDVDENALLRKTWSAMSNIPKLAAAIKDRGALNPGKNEPSDSFQKRPLMGDGGEWFIRYNVKDGVSPAPEADKKFKRAVARCNRCQSFAPFSSREAAIEHLQKHLRPGSHTQHVEPATTSSALPASDQSQPGSALAPTLNDWVVNSELLKREETNAGALAILRQACETAKDLFIQSRELADGVQNEDGTMSELYTLPRQLVEAFRSIAVFYLAIERALHHTEETYQNYDVLEATDDLSQRPYSEQGLEVLKRFGEGARRSLMMARAELCSMVASTTPFDILEHLSLGPEYVCAWLMRRLLVKPLQEHMTVGDMYREYLSTIQFQVNHRPGKRLLRSINLLQEELQVLSEVTTWQTKLIQNYMRVLDDSTYEKDMPPRRVMFPYERVLLRSCLDALDLTSEEYAELLRRCGPLSDSTKQSLEINEEDHGKAIMVFTTVTVIFLPLSFVTSYLGMNTADIRDMESLQSLFWIVALPLTLVTMATCLVVGYNGDEIRDAVAGAYRRVSGKQDRSTAARGISVAQRKRAGGRIQGTSSTSSSSPSASGGNNSQLDAAASNTFADDAEYANPRPEYAAGGGMDRYGSLRRRAATTPFTSSYPYPPPPPPPEPIVIEEQQQPVALGDMLFETRAAAPASYAKVQSFAEHIPISPPPLPSPPQNRRQALPTYNYEMPERRVRGPYGMPGPGPSTSRRRRGPDVYDRRVERVGDGGDEEREEDEIREYTWHRRRRGHEHDGGREQRRRIWREDMEADRRAEERRRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYW4</accession>
    <name>A0A9W8XYW4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">rRNA biogenesis protein RRP5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Ribosomal RNA-processing protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RRP5</name>
        <name evidence="13" type="ORF">N0V83_010632</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Involved in the biogenesis of rRNA. Required for the formation of 18S and 5.8S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="412781at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05702">
        <property type="entry name" value="S1_Rrp5_repeat_hs11_sc8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05703">
        <property type="entry name" value="S1_Rrp5_repeat_hs12_sc9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05693">
        <property type="entry name" value="S1_Rrp5_repeat_hs1_sc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05697">
        <property type="entry name" value="S1_Rrp5_repeat_hs5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05698">
        <property type="entry name" value="S1_Rrp5_repeat_hs6_sc5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05706">
        <property type="entry name" value="S1_Rrp5_repeat_sc10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05707">
        <property type="entry name" value="S1_Rrp5_repeat_sc11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000103">
        <property type="entry name" value="protein RRP5 homolog"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000155">
        <property type="entry name" value="rRNA biogenesis protein RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000196">
        <property type="entry name" value="rRNA biogenesis protein RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000278">
        <property type="entry name" value="rRNA biogenesis protein rrp5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000279">
        <property type="entry name" value="rRNA biogenesis protein rrp5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045209">
        <property type="entry name" value="Rrp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057301">
        <property type="entry name" value="Rrp5_OB_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057302">
        <property type="entry name" value="Rrp5_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048058">
        <property type="entry name" value="Rrp5_S1_rpt_hs11_sc8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048059">
        <property type="entry name" value="Rrp5_S1_rpt_hs1_sc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008847">
        <property type="entry name" value="Suf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 11 PRE-RRNA PROCESSING PROTEIN RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270:SF10">
        <property type="entry name" value="PROTEIN RRP5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24685">
        <property type="entry name" value="OB_RRP5_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23459">
        <property type="entry name" value="S1_RRP5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05843">
        <property type="entry name" value="Suf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="150"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="264"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="457"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="549"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="643"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="731"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="826"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="933"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="1041"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="1128"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="1222"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="1311"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="96"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1389"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1474"/>
            <end position="1493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1392"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1432"/>
            <end position="1447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4362538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1786" mass="196344" checksum="65C75AAAD632E446" modified="2023-11-08" version="1">MGAIKRKAEQGNTPSKKEKAPSSDRSAKRRKSDGAAEPSPAKAKSESAAPKSVFKDEETSFPRGGASVLTPLEHKQIQIKANQDVLFEQAGVKRSGGDGFSDMGSDDGEAAAPKSSKKKSSKKGKRSQEDGEQEQTIRAEGLSYKKLSLGTIILGQVTEITHQDIVLALPNNLVGYVPLTAVSDKLNERLEKLLKEEDADKEDGSDDEGFDDIELKDMFFVGQYLRACITATSDDTARARKRLELSIDPKLVNKGLSKRRIPVNSMIQASVVSNEDHGLVMDLGLEDPNLKGFLPKGELGSKIKHAKVQEGAVLMCLVTGLNSDGRIVKLSADQAKAGNLTKGNTMTEAPTIDVFLPGTAVDVLIADTTPATVTGKILGLLDATADAYHSGATEKGVDVTQKYKIGSKVKGRILFTCPDSEPRKVGVSFLDHVVTLSTRMSGKPKERKPPLELLPISTVVESAKVIKVQQGHGAFFDLGVRDVVGFAHISRLADDKVDILADDAGAFKLDSKHRARIIGYNELDGLFQLSLEQKVLDQPFLRIEDIKAGQVVKGKVHKLIADKTGATAVLVHLAEGITGLVPETHLADVRLQHPERKFREGVPVTARVLYTEPARHQIQLTLKKSLVNSDVEPWTSYSMLSEGATGPGILVNVRRNGATVRFYSDVKAWLPVAEMSEAYIEDATRHFTNGQVVNVRVISVNPEERQLLVSCKDPAAVDINKEAAFSALTPGAVVKGTVVEKSEETATLDLGHGVKGLLRIGHLTDGSEKKDVSTIKKIRVGGTLEDLVVLAKHDKSRTAVVSNKPSLRKAAQASMLPLSVDDLQAGEIVHGFVRGILPDKVFVELGNGISGPVFKSQLTQEMLLAPNFGVRKDQSVTARVTHVDAAKGFFWLSMKLEPEADEENAPKAPEPTGEALVNPIDTKISSTADINFGTTTTVRIRSVKSTQVNVALAENVQGRISVAEMFDSWDDIPDKKHPTAHFKMNDRIEAKVLGRHDARNHRFLPITHRSSNKTPTYEMTTKSDKIKTEFDVLSLDKISPGSSYVAFVNNIADRYVWVNISANVRGRIDFFDLTDDLEQLSAVEDNFPVGSALKVRVKAVDVAAGRLDLTAATTVTGKTLTLQDLKVGYILPARVTKLHDTSIVVQINENIAAPIYMEQLADDYDKAKPSEFKIGDIIRVAIIEIDIPNKKLGLSARPSRVLSSSLPIKDAEIKDKTQLKVHQVVRGFIKHIADNGIYVRLGPHAEAYVRVSHLSDEYIKDWKSAFHVDQLVTGKIISNKEHMRNPQMSLKTSVIQGDYAEPLEFSDLRNGQVVTAKVRHVEDFGVFLVVDNSNNVSGLCHISQLADSAVDKDKVKEMYKKDDVVKARIMRVEPKKRKISFTLKYSQVKGQDDDEDMEDASDDEVADEEGSDDGGVELDDDVDMRSVRSAESDDEIADADDSDEADEDVKPAGPGLSTSGFDWTGATLDFDERKGTAASESDDGAAKKKKKSKKAIIKEDRTGDLDAYGPQSVADYERLLLGQPNSAEMWVRYMVFQRELNEIEKARQIARRALATINPREEKEKLDVWTALLHLENDFASDDAMDEVFKEACQHNDAREVHERMIKIYIGSGKVDKADTLYQSMTKNKSFTPDPQFWLSYATFLMDVLSPPSPTRARALLQRATQSVASSQHRYLTQKFAALEFKSPNGDAERGRTIFEGLVSTWPKKGDVWDVYLSLEMSHGSTEGVRDLFERMSKVGGKSKRTSAVYKKWAEWESSVGNVKGVERVKALEEQWREAKQGKDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ83</accession>
    <name>A0A9W8XZ83_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CHS2</name>
        <name evidence="12" type="ORF">N0V83_010105</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ83"/>
    <dbReference type="OrthoDB" id="26569at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013616">
        <property type="entry name" value="Chitin_synth_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF38">
        <property type="entry name" value="CHITIN SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01644">
        <property type="entry name" value="Chitin_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08407">
        <property type="entry name" value="Chitin_synth_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="784"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="827"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="858"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="902"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1026"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1058"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin synthase N-terminal" evidence="11">
        <location>
            <begin position="350"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="134"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="98"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="295"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4362988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1140" mass="127563" checksum="518B842E021AC252" modified="2023-11-08" version="1">MDGPTTPGGGHYGRPPAYDDEDGLSPQMQGGSTMRLLTNVDESQRYMPRRSFESTSSMAGARSTTAALGERMTPSEVGKQKAAEADIVDFLREAGDALNSTSSAPSAAAVSHSHMPRRKTVRSTMVDHVPVLQSNMTRDPGLPLHPTRRPPSPVSRTYHPTASSISSLSRTTSVLDTAPNMPPPAEDSPSYVPYRRPLSPDRPAPGRPYSPTRTSVDYSRPPASVNSYEPVDLNGSPRPGTPSSRYGGGRPGSPKRPLPPAPLFAGGARPVSRDSEGTMDMTDMTSIPIDNDDPFTEHGDDRPELHSRDSYMTDDTYTDVYTADGEKVEHYGPAPDGAQVRRGAREAVMTKKEVRLINGELILECKIPTILYSFLPRRDDIEFTHMRYTAVTCDPDDFVDRGYQLRQNIGNARETELFICITMYNENEIDFTRTMHGVMQNISHFCSRMKSRTWGKDGWQKIVVCIIADGRGKVHPRTLDAIAAMGCFQEGIAKNHVNQREVTAHVYEYTTQVSLDSDLKFKGAEKGIVPCQMIFCLKERNQKKLNSHRWFFNAFGRALNPNVCILLDVGTKPGPKALYHLWKAFDTDSSVAGAAGEIKAAKGKAWLGLLNPLVASQNFEYKMSNILDKPLESVFGYITVLPGALSAYRYHALQNDHTGHGPLSQYFKGETLHGQDADVFTANMYLAEDRILCWELVAKRSEQWVLKYVKAATGETDVPGKTLFRIRLQTQANLSLDTVPEFISQRRRWLNGAFFAAVYSLLHFKQVWGTDHTIWRKILLHIEFVYQFVQLLFTFFSLANFYLTFYFVAGSLSDPQLDPFGHNIGKYIFYILRYTCTLLICMQFILSMGNRPQGAKKMFFWSMVMYGVIMAYTTFASFYIVIVQLKDPKAEKSLGNNVFTNLIISTATTIGLYFLMSFMYLEPWHMFTSSAQYFALLPSYIATLQVYAFCNTHDITWGTKGDNVAKTDLGDAKAKTKNVVELEMPSEQLDIDSGYDEALRNLRDRVEVPTPPVSESQQQEDYYRAVRTYMVVVWLISNAILAMSVSEAYSDRKVTNNFYLTFILWSVAGLAFFRAIGSTTFLVINSIHAIMETKLKWEDKMEDKKSRTGLGGGRRKFGKFGGDGVVAAVAGGVGVRFRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y035</accession>
    <name>A0A9W8Y035_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_010004</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y035"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11065">
        <property type="entry name" value="CYP64-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050364">
        <property type="entry name" value="Cytochrome_P450_fung"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46300:SF2">
        <property type="entry name" value="CYTOCHROME P450 MONOOXYGENASE ALNH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46300">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4362887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="543" mass="61769" checksum="67CDAA092380E4B1" modified="2023-11-08" version="1">MFDTSYAPIFVICVVALIAHRLSKLGRRPAGYPPGPPTLPIIGNLHLMPKKQAHLQFQKWAEEYGPVYSLILGTKVMIVLSSDQAIKDLLDKRSGIYSSRPEMYLGQIVSGGLRMLLMEYGDTWRMIRKIVHNNLNIKAARTYVPYQDLENKAMLVGFLEQPDLFVDHIRRYTNSLTTQMIFGFRTTSIDDPKLKQLFHGFEKFSEVQGTQMAALLDLFPVLRALPDFALPLRKYAKKLHEKESELYVGHWLRVKQAIKNGTARPCFCLDLVRAQDEQGFSDPLAGYVSGSLLEAGSDTTAAILVGFIQAMVLFPNVAKRAQEELDCVCGDRFPTVEDEPNLPYIRACVKESMRWMPTVVMGVPHAVIRDDEYLGYTIPKGAGVMWNVWAIHMDPKRHSDPRRFDPARYAHDNQTAAEAANNPDATKRDQFIFGAGRRLCQGMHIAERSLFLAIARLLWGFDFHRARDANDREVVPDAHDLTEGMFVLPKPFLARIVPRDQNKIVQVKAEWSKMQDLLDEDQQWKVLPEGLIWKEYKQAAKAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0T1</accession>
    <name>A0A9W8Y0T1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">5'-3' DNA helicase ZGRF1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009737</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0T1"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052800">
        <property type="entry name" value="DNA_Repair_Helicase_ZGRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018838">
        <property type="entry name" value="ZGRF1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28535:SF1">
        <property type="entry name" value="PROTEIN ZGRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28535">
        <property type="entry name" value="ZINC FINGER GRF-TYPE CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10382">
        <property type="entry name" value="ZGRF1-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="5'-3' DNA helicase ZGRF1-like N-terminal" evidence="2">
        <location>
            <begin position="23"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="412"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="285"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="745" mass="81236" checksum="D10431C86FBF564E" modified="2023-11-08" version="1">MTAPLRGTPRSSALPASQNTAPVAEFRCLFTHDVRRKQKRWQDGYLKFHTFNNRVMVYDQSRNFLGDTYYKDSNELHEGDELNLDKGVMIEVAEPMGVTQTDLTPLFEKKPKEPPVRPNAASQTRPFQRPTSVAPQTTSQLRHKSLNSLLGTPRGPIGKSVPLKSPYEARKEKENNIVEERPPKRQKTTPQGGILEGIQSSLRRKPRAKVIVLDAEPEPAPAFFSDVTLPSTPPGHVRPRSEPSPQPQPIVKPVVIEKPPVQTPKIPQGKVPVPSVKAVETPKQSAPTSSPPVSASNRIANVNFAVQPARKQPKEPSPTASPPRNPKTNPKTKSLRLTTGVKRGTLLCQSLPQPIAPLIEGKRKGPRKVKEGAAKRAKVSASPPSAFLGVVDDPEVVHGIMDQQILVPLSPVQPQELTSSPAFDDPRPLPTIATRKVGAKEAVRKQAAPAPSEPQIAKPKLPSPKSTSQNALAQKKAIEKPQPIRKASPTPVHAFEPPTADSRDTSPAHTEAPDTRSRNSTVSPKKKIPLSTGGFRKKPQRSSKQSTVPPTSEVIVPSPRNESVPLPPHPLRASKKGPVMTTSELAALLQKPKRAKKAPINDPIEDDGTQGAAAGKSPNRNFRRVRSENDAPIPSTAEDWEKRNLPKTPPSNNLTDIVVVDEPDITVVGAADVVPVVTTRKNNGLSALIKKTDPRRKFQRTQSLSVQTNIPPVEVDIPSPVVDQDVGPWSTEAYDLFDWRPPGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y113</accession>
    <name>A0A9W8Y113_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_008996</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y113"/>
    <dbReference type="OrthoDB" id="5321960at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000077">
        <property type="entry name" value="Probable chitin synthase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF46">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03142">
        <property type="entry name" value="Chitin_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="456"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="486"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="519"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="705"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="714"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="745"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="791"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="863"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4364402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="877" mass="98972" checksum="52BB33455408EA77" modified="2023-11-08" version="1">MVWHEMMGVFARADASIPDITDPWPVKDIVYVSIIGTVMLAALLEWFLWVAAFMYCIVKVFQKAETISVRILAIFFGILFFLLRAIFLPIMVVTLPLPSQVVKYFPQQMVDFLQWFAFWSFAGLLTIPWLFCVYQLVTHSVGRTRRIKSVLDEASAPKVVIVMPCYKEIPEILLRTCDSLVDCEYPPSCLHIFLSFDGDQEDELYLNTIEKLGVPLTLDSYPKSIDVIYRSCRITVSRFPHGGKRHCQKRTFKLIDKIYTEYIKRNDNLFVLFIDSDCILDKTCIQNFMYEMELKPGSKKNMLAQTGVITSTTEKNSLITLLQDMEYVHGQLFERSVESGCGAVTCLPGALTMLRFSAFRKMAKYYFADKAEQCDDLFDYGKCHLGEDRWLTHLFMIGAKERYQIQMNTGAFCKTEAVQTYQSLLKQRRRWFLGFITNEVCMLTDIRLWKRYPILLIVRFMQNTIRTTALLFFILCISLITTSQKVANLPVGFIAISLGLNWLLMLYFGAKLGRYKIMLYPLMFIINPFFNWVYMVYGIFTAGQRTWGGPRADAGAADTSKTPQQAIEQAEATGDDLNVVPETFKASAEAHKHRPAPIPLQPSDNIEGRFAPAERLANGWYQQGNDSGLTLPNTLPRNPNVPNVPLHPRSSMDSFMSSGTSANNSIYMPRRVESFMDPEDARIYHKTQQTQKPAGGAFFEEARQGPYDVGRSGSGKSPYQESVDSLSDGDSVYHSKGPQRPQAPRMNSDNGGYSGYNTPPSGARTPTGNNSPLSAEYHNQSSGLEAPKPSYNRTTSGDQQQRSGRSPLARQSYVRTAPGEGIELQAPVQQQSSLMRDVSPAPLGQPSMPAASNHQRSESADSKKRKRLSKAPPQGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1B3</accession>
    <name>A0A9W8Y1B3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Haloacid dehalogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009689</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1B3"/>
    <dbReference type="OrthoDB" id="444127at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051540">
        <property type="entry name" value="S-2-haloacid_dehalogenase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01493">
        <property type="entry name" value="HAD-SF-IA-v2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43316:SF9">
        <property type="entry name" value="ACID DEHALOGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G14460)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43316">
        <property type="entry name" value="HYDROLASE, HALOACID DELAHOGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="247" mass="28022" checksum="589645E484193B9B" modified="2023-11-08" version="1">MSKLTDFKLLSFDVYGTLIDWEGGALTALEPSLQKSGKTFNDPGLDQKHILQVLHEFEPVAERENPKLKYSEILTICHPQLCKKLGLAEPTKEESEKFGASVGSWPGFPDTVDALKRLSKVYKLVVLSNVDNESFKAGNAYGLQGFKFDAVYTAEDIGSYKPDLKNFEYMLKHVKEEFGVEKHEVLQTAQSQFHDHHPAKDIGIKSSWIYRPGAVMGNRKDPVYNWKFDTLGDMADFVEKELEEQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1G6</accession>
    <name>A0A9W8Y1G6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1G6"/>
    <dbReference type="OrthoDB" id="3050608at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="66" mass="7393" checksum="5963930E0FCAA764" modified="2023-11-08" version="1">MNTNNQGGAPQKDYLDKGLEMAQKKFGGAQGQKMSQNRNLNEKITDGMRKAYEKLTGKKVNPKYSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1K6</accession>
    <name>A0A9W8Y1K6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pectate lyase C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_010231</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1K6"/>
    <dbReference type="OrthoDB" id="302705at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052063">
        <property type="entry name" value="Polysaccharide_Lyase_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42970">
        <property type="entry name" value="PECTATE LYASE C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42970:SF1">
        <property type="entry name" value="PECTATE LYASE C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040984213" description="Pectate lyase C" evidence="3">
        <location>
            <begin position="19"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4363111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="402" mass="42345" checksum="2C44FF1B466A7492" modified="2023-11-08" version="1">MQFLWFLPLLTSTVSALAFPGAEGFGANAIGGQNGKVYVVSNLNDSGTGSLRDAVSQPDRTIVFSVGGLIQIKDRLVVSKRISILGQTAPGDGITVYGNGWSFSNADDAIVRYIRIRMGKSGASGKDGITIAEGSNMIFDHVSVSWGRDETFSINGNDVGNITIQNSIIAQGLETHSCGGLMQTNLGNGISLFRNLYIDNKTRNPKVKGTNDFTNNVIYNWGGGGGYIAGDSSAASEANIVGNYFISGPSTSVTAFTRGNENFKGYVEANFYDSDKDGALNGAQLGVSSSNYGGMVIQTTRFPFPAPSKTLSAPDALKLAEASVGASKVRDAVDKRLITELQSYGKTGELISDENASPMSGPGTIDGGTQWVDANGNGIPDDVESQFKTVEDWANSLVPSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1N1</accession>
    <name>A0A9W8Y1N1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycine zipper 2TM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_009594</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1N1"/>
    <dbReference type="OrthoDB" id="3801381at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051407">
        <property type="entry name" value="Bact_OM_lipoprot/Surf_antigen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008816">
        <property type="entry name" value="Gly_zipper_2TM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35603">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35603:SF2">
        <property type="entry name" value="OUTER MEMBRANE LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05433">
        <property type="entry name" value="Rick_17kDa_Anti"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycine zipper 2TM" evidence="4">
        <location>
            <begin position="230"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="14"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="262"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="78"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="99"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="294"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4364139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="319" mass="36648" checksum="F9FC2AD2F6A0B8A8" modified="2023-11-08" version="1">MADYEELVELGFEGFNKVTDKYHDRAYDRLPNMKRKKKRESQDRAKAEGQSDQQQQQQRSPRQSDDNNSRPRSGPPRDSPRDRGAKEYRSDSKMYSPERGQERDSRGASERYYDNVGFNSDGPNTGATPPRDRDDYSKSRGFEVAPYQAPPMQPYQAPPMQPYQAPLMQPYQGPPPGYYDQSRGQGDRGRPGTQRRGSSWSPPRPKKHDRDSHKPRARSKSGDKQQRMIATVGGAIAGGFAGNQARKGKKYDTVATIVGAIVGGMGAREASEFWDDRKKKREEKQDDWEEDFGKDERGSERGSRRRRDDRDDRDDRRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2H6</accession>
    <name>A0A9W8Y2H6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2H6"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="255" mass="28905" checksum="2107CFA403C40ADD" modified="2023-11-08" version="1">MSSQDRPTYNNLSALLSYNQQSARLGSHNNRATTPTPATLNPNPEKQKKKTALGADNNTTNNGGAERYNTANTFALPLRVVAPPNQRTSLKPSTPTRNDSVLHRRSLASHQDLAQFRARLGLRPVREALLGEPWGSSGSAEDDQDDDDDDDDDDDEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEVVVVEEREDDIIWDCGGVPVAYAIILEDIGWICLEGPECGCVAEHNFVLQVDDEDRIWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2Y3</accession>
    <name>A0A9W8Y2Y3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2Y3"/>
    <dbReference type="OrthoDB" id="3795810at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="76" mass="8495" checksum="3676FF38973BC824" modified="2023-11-08" version="1">MSTTTTIKLPTMTPAQEATAAFEREKAAITSYEEGWDRWDGPVWPVGYVQCRMQEDGYRSVPPTPSEEDGAAKKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2Z1</accession>
    <name>A0A9W8Y2Z1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="9">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_008360</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2Z1"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF103">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MANNOSIDASE MNL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="74"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="444"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="762"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="531"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="650"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="844"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="930"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="607"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4365740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="939" mass="105760" checksum="708CEE14BEA91511" modified="2023-11-08" version="1">MFRYRRYRVFLAFAFIAVFALYKFGTSGASWREAASHAAGLKSEAENAPQVDTKPRPAVAHETRSPKLDIAVANSQQPLQTPPPVKERPQPSSSPVVTRKQQKPTVPTPIRPRPPHARPNHPGHGDALSTSIEAVHWTKLPEHFPVPSNSLIRLPSGKTKPMPKIQFQFKPEDAATKADRLSKLEAIRGVFKKSWSGYKEYAWLHDELRPESGTYRDPFANWGATLVDALDTLWIMGMKEEFEEAAKAVDTIDFTTTPRADIPLFETTIRYLGGLVGAYDISGKKYKNLLDKAVELAEILISAFDTPNRMPETYYYWRPQFASQRHRASTRVVLAEIGSLSMEFTRLAQLTGDDKYYDAIARITDYLEEFQNYTRLPGMWPTYLDASGCGKVYVDLASQEPLVAQADLLAEESREDTPTTTELLSPEGKKYVPLNLPDPIVLTPNRAPSENHDSTVPGTAAKDKIRDWNDDPLEKRQLDIDLDEPPTIAPDAAKTSSAKAAVPTPPECVAQGFVSTSDYGREEFTLGGMSDSTYEYLTKEYLLLGGHVEKYRTMYEQSMDVVKEHLIFRPMLPHGDDILFSGKLNVPSNVDDTKVGDLEHENAHLTCFAGGMFGMGAKLFDRPEDLEIAKKLTEGCVWSYDMTASGIMPESFEVARCESMKECAWNETAYWEVLDPRSEYRLQNYKEQLKGYKKQLVSASSWYEAQLAAMTASPSPSADAIFETQATPTLFYADTLDKRQLADLEDDMDVAVTPAPSVPIRQDRESVMGGEPEEGEGPPTKVQPTLDIEEVALQPSLTLPAFPMLYSPKPPLSHEEYVQSRIKEERLPTGVTRIGARNYILRPEAIESVWYMYRITGDSHWREAGWRMFLAVNEHTSTVYGNSAIDDVTQTSPQLNDEMESFWLAETLKYFYLLFEEENVLSLDDWVLNTEAHPFERPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y360</accession>
    <name>A0A9W8Y360_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GTP-binding nuclear protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GSP1</name>
        <name evidence="9" type="ORF">N0V83_007888</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the small GTPase superfamily. Ran family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y360"/>
    <dbReference type="OrthoDB" id="48625at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00877">
        <property type="entry name" value="Ran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000131">
        <property type="entry name" value="GTP-binding nuclear protein Ran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002041">
        <property type="entry name" value="Ran_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071:SF0">
        <property type="entry name" value="GTP-BINDING NUCLEAR PROTEIN RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071">
        <property type="entry name" value="RAN GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00627">
        <property type="entry name" value="GTPRANTC4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51418">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4366252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="219" mass="24625" checksum="B9A1BF5411B76FEA" modified="2023-11-08" version="1">MAETAAQGNIPTFKLVLVGDGGTGKTTFVKRHLTGEFEKKYIATLGVEVHPLGFTTNLGPIQFDVWDTAGQEKFGGLRDGYYINGQCGIIMFDVTSRITYKNVPNWHRDLVRVCENIPIVLTGNKVDVKERKVKAKSITFHRKKNLQYYDISAKSNYNFEKPFLWLARKLVGNQTLEFVAAPALAPPEVQVDQAVLEQYQKEMQDAAQMPLPDEDDADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3R5</accession>
    <name>A0A9W8Y3R5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">amidase</fullName>
            <ecNumber evidence="3">3.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_007371</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a monocarboxylic acid amide + H2O = a monocarboxylate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:12020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35757"/>
            <dbReference type="ChEBI" id="CHEBI:83628"/>
            <dbReference type="EC" id="3.5.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.4" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3R5"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072:SF4">
        <property type="entry name" value="AMIDASE C550.07-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072">
        <property type="entry name" value="AMIDASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="7">
        <location>
            <begin position="77"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="5">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="226"/>
            <end position="229"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4366843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="540" mass="59739" checksum="284E1DAE98C3D851" modified="2023-11-08" version="1">MVKAWEEIAKEAQDYRASTIAQLSPPAPTVPSELPKDVTNVPGQLLDQHVLAITNLLPEQLLPAIASGHLSAVKVSHAYLQRAAVSQGLTNCLTELLPNRAFQRAKELDAYLEEHKKPIGPLHGLPISVKEHIGLQGLRCTTGYVSHWDNIALEDAHILQILQEAGAVFHCRTAVPQTMMHLETDSNLYGVTSNPYNRNLTAGGSSGGEGAVIALRGSCLGIGSDVGGSIRSPAANCGIYGLKPTAFRLPSDGWGYMMAGADTVETVLGPLSTSLEGLKIFMKSIIDSQPWLTEPALIPMPWRPYTIPQDRPLKLGLLWSDDIVRPHPPVNHALQMIAGRLQEQGFEVCNFPPYLHDEAWAILASLYYPDGGEADSEDIAKSGEPWRPLSEWIIKENSCVKKLSVGELAYWFEEREAYRKEYALHWNKYGIDALLCPVGPGVAPKHNTAKYWNYTSQWNLLDYPGVVFPVCHVDQVEDLSPDYRKYMSGKDEENWKLWDPQEFHGAPVGLQLVGRRFEDEKILGILEHIKAKIGLPLKDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4V7</accession>
    <name>A0A9W8Y4V7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_006847</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4V7"/>
    <dbReference type="OrthoDB" id="2985014at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000013">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF9">
        <property type="entry name" value="MAJOR FACILITATOR-TYPE TRANSPORTER HXNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="376"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4367266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="436" mass="47897" checksum="72EED060B99B4D44" modified="2023-11-08" version="1">MGSPKDAETGTEGRTMSIEVQHAAADDNGSMHKGPISDTLYLDPVAERSYVRKLDMWLLPVLAIMGIVSLCQASVKNYAGILTCRILIACFQSGFFSGCIFYLTLFYKRDELAFRTAIFYGTATIAGAFTGLISYGVFQIDSHLAGWKILFLIEGAMTVIWGAVSFWLIPSGPADCKWLTPAEKEAARTRLMLDSTAVVNSKYSTKAGLKALTDPIMWPFMLLCISYGVATKSTGTFLPQIVARLGYSTIKTNLYTVAPNLVGCIFTLCVTASSDRFQERTMHLCLGLSFTMVGYLMLAGLDPLKNPGPSYAGTFFLSMGTYIPSCLVHSWHNNIKTDENSRAMVTGVMVGFSSVGTIISAQLFRDEWAPKYTQALAGTGSFQGLAIIITAGLGLYMRRENKRRDRVMGVEAMKPGDVPMCDLKDGQKDIRFRYWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4W9</accession>
    <name>A0A9W8Y4W9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATP citrate synthase</fullName>
            <ecNumber evidence="2">2.3.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">ATP-citrate (pro-S-)-lyase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Citrate cleavage enzyme subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">acl1</name>
        <name evidence="20" type="ORF">N0V83_006862</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the formation of cytosolic acetyl-CoA, which is mainly used for the biosynthesis of fatty acids and sterols.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>oxaloacetate + acetyl-CoA + ADP + phosphate = citrate + ATP + CoA</text>
            <dbReference type="Rhea" id="RHEA:21160"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.3.3.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Composed of two subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.8" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4W9"/>
    <dbReference type="OrthoDB" id="3261737at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003878">
        <property type="term" value="F:ATP citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06100">
        <property type="entry name" value="CCL_ACL-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000003">
        <property type="entry name" value="ATP-citrate synthase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.230.10:FF:000005">
        <property type="entry name" value="ATP-citrate synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000024">
        <property type="entry name" value="Probable ATP-citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23118">
        <property type="entry name" value="ATP-CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23118:SF42">
        <property type="entry name" value="ATP-CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="20" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="20" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 20" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CoA-binding" evidence="19">
        <location>
            <begin position="47"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="8"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00062455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00076189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00083544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ4367281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="668" mass="72512" checksum="43E5D849047BFEC7" modified="2023-11-08" version="1">MVSAAPVNGTTPQQAATSTSEGLTPNDNIRRFAAPSRPLSPPAAHTLFHPKTRCFVYGLQPRAVQGMLDFDFICKRKTPSVAGIIYTFGGQFVSKMYWGTSETLLPVYQSVEKAIGKHTDVDTVVNFASSRSVYQSTMELMEFPQIKSIAIIAEGVPERRAREILHVAQKKGITIIGPATVGGIKPGAFKIGNTGGMMDNIVASKLYRPGSVSYVSKSGGMSNELNNIIANTTDGVLEGVAIGGDRYPGTTFIDHVLRYQSDPDCKIIVLLGEVGGVEEYRVIEAVKSGQVTKPIVAWAIGTVAGLLKTEVQFGHAGSFANSQLETAATKNKSMREAGIYVPETFEDMPSTLAQVYQKLHKDGTIKPKAEPVVPKIPIDYSWAQELGLIRKPAAFISTISDDRGQELLYAGMPISDVFREEIGIGGVMSLLWFRRRLPNYASRFLEMVLMLTADHGPAVSGAMNTIITTRAGKDLISALVSGLLTIGSRFGGALDGAAEEFSKAFDKGLSPRDFVDTMRKENKLIPGIGHRIKSRNNPDLRVELVKEYVKKNFPSTKLLDYALAVESVTTSKKDNLILNVDGCIAVCFVDLVRNGGAFTNEEAEDYLKMGVLNGLFVLGRSIGLIAHFLDQKRLRTGLYRHPWDDITYLLPSLAGGAPGNEGRVEVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y544</accession>
    <name>A0A9W8Y544_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006968</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y544"/>
    <dbReference type="OrthoDB" id="3643156at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040992318" evidence="1">
        <location>
            <begin position="20"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="387" mass="43392" checksum="8DC74E65D8C7690B" modified="2023-11-08" version="1">MKLLSFCVIGAASAHVVRSQEQQVPIVGHIVEDVAPRVLLGEDDVKDFAPSIGIHFTTSYAVAAARYQNGTIRDLVKVRGDAEYIELMTRWVKTRQSWREDCNYIASRPSGQFQLGRGKCNWRKVSRGVRGLLGLPLSRDAATLSSFLHIVKMKIEEEMGEPVTAIAPTIFPLDPAKKQDFQAALDLTGLTSTRSQDGYDPVVYHDINAAYAGLGYGFCEEWTIKENCTWEESRLLYFDVLFLSFDNSSFTVTVDYLRKASRELNTYNGNFSSDLGWWNLPIDEVPRARFWAQIHEMIVDTVSAWSHPPGRIVLMGEHGGDEEFRGVVEAALWSVMEVDADLMLQANELEDTTRLAARGAAEMAWRAQYLLGTDKEQKIAAQASLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y610</accession>
    <name>A0A9W8Y610_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Pre-mRNA-splicing factor cef1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CEF1</name>
        <name evidence="11" type="ORF">N0V83_006967</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CEF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y610"/>
    <dbReference type="OrthoDB" id="1410009at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11659">
        <property type="entry name" value="SANT_CDC5_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047242">
        <property type="entry name" value="CDC5L/Cef1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021786">
        <property type="entry name" value="Cdc5p/Cef1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047240">
        <property type="entry name" value="SANT_CDC5L_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45885">
        <property type="entry name" value="CELL DIVISION CYCLE 5-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45885:SF1">
        <property type="entry name" value="CELL DIVISION CYCLE 5-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11831">
        <property type="entry name" value="Myb_Cef"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="7"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="53"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="53"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="239"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="241"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="284"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4367385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="783" mass="87422" checksum="D8CF8C8A7AD48D7E" modified="2023-11-08" version="1">MVVTKVGQWSNVEDEVLKAAISKYGLNQWARCASLLAKKTAKQCKARWSEWLDPTLKKTEWSREDDEKLLTMAKLLPTQWRTIAPIVGRTATQCLERYQKLLDEQEARESGDLGLAGPEGGEAAAPSAEEVRRLRPGEVDFYAESRPARPDAIDMDEEDKEMLSEARARLANVSGKKAKRKARERQLDETRRIAQLQKRRELKAAGINIKITPKKGNHIDYNADVPLEKEVPAGFFDTTEEIDRNEKQRDAFDPRKQQLANKRKMDQQEEGGDSKRKKKDEKSGGLAASYAKAAQAQRIREAEQSSKRRALVLPSPQVGEAELEDIVKMGMIGERAIAAGGDDNIATQGLVGNYSNIIGNTPIRTPMAPKEEDFIANEVRNARLRTETQSALLGGDNPDLVDDEAPTSLSGPASRHQIVTPNPMATPYRQANGGVGATPIRQGPGATPMRTPRDTLRLNEEESGMQLVGQTPRDIRLRESAKHQDLQSSLAALPNAKDENWEFDLPEEQETQMEIEISEEDAAEIDRRKEAARQAAESAEFRRQTQVVQRYLPRPSLVDIDAMLQRALDLSDPVERVVETEMALLIANDVSKFGGGRVTGSLQPVERMTDEALRAAKMELALELAMTSDKDKKAFHSDFGTTWTNLHGPAILPGIAGYEEDEIDEHQLLVEAFDNAQESIIESAEQANKIEKKLALHHKGYINRAALLREKVGGASGALETAKRELDAGRTMQYSEQIAIDRRLESLRAEVQFVTKREREAQELYRSRRDELESLQEPVNGVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y637</accession>
    <name>A0A9W8Y637_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006392</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y637"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4368037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="191" mass="22046" checksum="2E8CD2C0B2BFBB25" modified="2023-11-08" version="1">MAGRVSPPSWKDLGYSDEAVSLEKYKIWEEHNNRIEKGTNVWIITIRENPFWDEAYQIIGSGRGYTTGPSTVKWCLTNKRWFAQGYGIRVTVDGTVDGKVSDEKTLEELKRIIKHLDTNAYWFGQSWPTNSAALVGRESCPWGMSFDLKIERTRYEHGRHSITEGEKLFSLTLQEEMEFGEDDIVESIEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6H6</accession>
    <name>A0A9W8Y6H6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_006848</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6H6"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044283">
        <property type="term" value="P:small molecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00682">
        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="3">
        <location>
            <begin position="206"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4367267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="379" mass="42286" checksum="8E3F7ABFD6113C57" modified="2023-11-08" version="1">MSYTGMYDKGDHIDTGIGGTRKINTAGKPSSGTFSSIPVIDLTDATSPSLDARKKVAQEVYEACVNVGFFYIKNHGVPDEAIASIKHEAKRFFHEQSEEDKMNLDISKNTEFYGYAPIKPKLENGRAKTRMFESINFGYEPSMDPGASTTEDNGPNFWPSEEKLPNFKANVGKYYSAVMDLSRQLLHMFALSLNLEETFLDKFCKKPGVLLKLNHYPAVVPDSSDSAGIHAHSDLESKRLRFTYQVELESLHSPPGFTILLQDDVKSLEVLSKDGHWIPADPIPGTFVVNIGDAMSMWTNNLFLSTIHRAYNKEGKVRYSVPFFFGADYDAVMETLPSCIDEMRPVKYKPITAGEYVRMKLNNTYPKAVGKVAAEVSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6L8</accession>
    <name>A0A9W8Y6L8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6L8"/>
    <dbReference type="OrthoDB" id="5337308at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040815307" evidence="1">
        <location>
            <begin position="23"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="365" mass="40494" checksum="579AFD7338B3A214" modified="2023-11-08" version="1">MKLSTTTLIAAIVSSSPPPALAFDSSDLADPQDWTRYTLKGGALLCALHATDQLAGRQLHDTRNPPSAASIWTGDLKQALETWYWHDVPPTEPSICDIAGYWKIPFAVRSLGLDSRLKVEGGDNACYRVEHWDPGMKDGENGRQVPAINQWYNVDGREYRSTGAHYEFATNPRGGALYTFYLESPKSSASSLWYNSRKDASPDDLPSLRALSDVLWGFWNRENENVKNIRYFFVLGVTNEVTNQLIATCLQNAGGKELSDWPGTSFGTGTEEGHALLGSPNGATFAYFLLQHKAQLGYKSISKVTVFPPETDDDVAFVDPALVFHVEDVTDLLHDEAEEEVKKDAGDRRAGTVLRIHKLHNSNWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6S9</accession>
    <name>A0A9W8Y6S9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphorelay intermediate protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">YPD1</name>
        <name evidence="3" type="ORF">N0V83_005864</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6S9"/>
    <dbReference type="OrthoDB" id="1673781at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043424">
        <property type="term" value="F:protein histidine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00088">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.160:FF:000007">
        <property type="entry name" value="Multistep phosphorelay regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="entry name" value="HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045871">
        <property type="entry name" value="AHP1-5/YPD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28242">
        <property type="entry name" value="PHOSPHORELAY INTERMEDIATE PROTEIN YPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28242:SF52">
        <property type="entry name" value="PHOSPHORELAY INTERMEDIATE PROTEIN YPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="Histidine-containing phosphotransfer domain, HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HPt" evidence="2">
        <location>
            <begin position="27"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="1">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="140" mass="15837" checksum="06DD9E2CCFFAA1DA" modified="2023-11-08" version="1">MSIPGAEDTIDPATFEQILEMDEDEVEREFSKSIVYDFFGQADSTFKKMDSSLEKKEGDYLKELSELGHFLKGSSATLGLTKVKDSCEKIQHYGSLKDETGVKDIKEKEACEKLKTTIAQAKEEFVEVKGVLKEFYKDTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y726</accession>
    <name>A0A9W8Y726_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dicer-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">dcl1</name>
        <name evidence="25" type="ORF">N0V83_006523</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="25">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="17 18">Belongs to the helicase family. Dicer subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="416741at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050688">
        <property type="term" value="P:regulation of defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18034">
        <property type="entry name" value="DEXHc_dicer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd18802">
        <property type="entry name" value="SF2_C_dicer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000015">
        <property type="entry name" value="Dicer-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001669">
        <property type="entry name" value="Dicer-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001988">
        <property type="entry name" value="Dicer-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.380">
        <property type="entry name" value="Dicer dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038248">
        <property type="entry name" value="Dicer_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005034">
        <property type="entry name" value="Dicer_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056755">
        <property type="entry name" value="DSRM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950:SF62">
        <property type="entry name" value="DICER-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950">
        <property type="entry name" value="DICER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03368">
        <property type="entry name" value="Dicer_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24995">
        <property type="entry name" value="DSRM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51327">
        <property type="entry name" value="DICER_DSRBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-0930">Antiviral protein</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="13 18" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="22">
        <location>
            <begin position="119"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="23">
        <location>
            <begin position="444"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="Dicer dsRNA-binding fold" evidence="24">
        <location>
            <begin position="643"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="domain" description="PAZ" evidence="21">
        <location>
            <begin position="902"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="domain" description="RNase III" evidence="20">
        <location>
            <begin position="1046"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="domain" description="RNase III" evidence="20">
        <location>
            <begin position="1276"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="25"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="46"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00025403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00035116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAJ4368167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1578" mass="180219" checksum="0D1AD657FA86DD59" modified="2023-11-08" version="1">MARAEVDEEQGNFVSCDQIDASSLAIGEVKHADDHEQADEDDESDIDSHGPKSTSEKRRAQNKVMRAFAANISAHISKEEVKEAASKSVNEEQLSIRDILAKQETTVRITNPRDYQTELFQRAKDENIIAVLDTGSGKTHIATLLLRHILEKELEDRANGSIHKIAFFLVDSVNLVYQQANVLRCGLDQSVEGICGAMGASLWSKSTWEKHFSNNMVIVCTAAVLVDCMMHSFISMAQINLLIFDEAHHAKSNHPYARLMKDYYAQEHDVSKQPRIFGMTASPVDVKGQSSEHIREAARDLEKLLHAEIATSADSTLASNSISRPDEEVAVYARLRNNYETPFHVEVKARYGDVSAFHKFFVASSRIASDLGRWASDMYWSFAFADEQSSKLQNREQFKYNKITKDGSQEEWDRKIKRLEEAAGFVQKQNFGIPTLSDQDVSSKVQQLHYWLNLYYERSDEARCIVFVEQRQTARLLKLIFGHIGGSNLHCDVLVGVNNRAGEHNVSLRNQILTVSKFRRGELNCLFATSVAEEGLDIPQCNLVVRFDLYRTMIGYVQSRGRARHRNSKYLHMLEADNNDHRERLLDVRTDEHVMRNFCKGLSHERKMEELGKDGSELLAFEDQLFPSFTDPQSGAKLTYRSSLAILNHFVATWPYPNHATMLQPNYVTSPEMSYDPRDPQRRGFVCEVILPEGSPIISITGDVQSRKAIARCSAAFKMCLELHDKGFLNEHLLPTTAKLFPAMASAHLAVNEKKKGKYQMIIKPFFWKQGRDTVPERLYLTVVHVDAGLDRPHQPLALLTRNPFPQLPSFPIYLTDGTASNVISQSSSKPLPVAAESLEIFTKFTLRLYEDIYNKVYDHDIRKISYWVLPILKTRMSALPHITSVEKILDMEQIHKVCKEAIWTWTSETKAEDLIDKYYLDPLHGGLRYYSNRLAPHLRPQDPVPTHVPRQNHKFMKNILEYTDSRWSKSRDISRWDQSQPVLEVEWIPFRRNHLARVEDNEGKELRNLRAYICPQPLRISNLAVPFVAMCYILPVIIHRFESYLIALEACQALDLNVNPALALEALTKDSDNSEEHGEEQISFKSGMGKNYERLEFLGDCFLKMATTISVFVQQPDEDEFEFHVRRMEMLCNKNLMETALGKKVHRADDPERNLQLYKYIRTEAFSRRTWYPEGLKLIKGKGMNKSEDDWLKLTHNLGDKSIADVCEAFIGAAVMEDHEDGPWNPSQWDQAVKAVKLFVNSQDHLMSKWSDYYAAYDKPQYQVADSTAAMIDMAQQIERTHPYHFRYPRLLRSAFTHPSYPYMFEHIPSYQRLEFCGDALLDMAFITYLFHKYPDKDPQWLTEHKTPMVSNKFLGAVCVNLGWHTHIKQNTAVLSTQIRDYVTEVSEAQREAGPGAVDYWVNVPEPPKCLADVIEAYVAALFVDAEFDFSVVQHFFDLHLKPFFVDMTLDSYEHFASNHPTTRLSRLLSINFGCSEWRLGALETDTIIPGKGKAIAAMVLIHNKVSFHSLGQSGRYARVRASQAALETLEGLPPYEYRRRFGCDCVGEGEGMGAGSSEVAVRAKKAQMKEAMGPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7S5</accession>
    <name>A0A9W8Y7S5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 subunit J</fullName>
            <shortName evidence="5">eIF3j</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 30 kDa subunit homolog</fullName>
            <shortName evidence="5">eIF-3 30 kDa subunit homolog</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">HCR1</name>
        <name evidence="7" type="ORF">N0V83_005007</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eIF-3 subunit J family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7S5"/>
    <dbReference type="OrthoDB" id="20381at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.60:FF:000003">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.60">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03009">
        <property type="entry name" value="eIF3j"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023194">
        <property type="entry name" value="eIF3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013906">
        <property type="entry name" value="eIF3j"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08597">
        <property type="entry name" value="eIF3_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="107"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="225"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4370486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="270" mass="29954" checksum="DFDB2CCD20BBF8E0" modified="2023-11-08" version="1">MAPGKWDDEDEESTPPSSPAAAPIARRSKFDDEEEDSDVLESWDAAEDSEVEREKAKKAAEAKAKADAEAKANHKSKAQRIEEHRQAQLRRRQMEDDMSSDEDETEAERRARLKQQELDGSRAQAEELFGDLSVSKKRTGKAVTLTDESDPGNAIDLSSLSIFNPNTKDQFTKLRDTLTPLIAANSKKGQYSLFLQEFTKQIAKDLPSEQIKKIASGLTTLSNEKMKEEKAAEKGGKKTKAAKTKTTLNAARDVGVRADTKAYDDEDFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y818</accession>
    <name>A0A9W8Y818_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Decarboxylase AgnL1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">AGNL1</name>
        <name evidence="3" type="ORF">N0V83_005520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tpcK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y818"/>
    <dbReference type="OrthoDB" id="3454835at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009799">
        <property type="entry name" value="EthD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07110">
        <property type="entry name" value="EthD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EthD" evidence="2">
        <location>
            <begin position="34"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="156" mass="18233" checksum="3A24D5F62E47FEFE" modified="2023-11-08" version="1">MGGISTTNDPEMPPTPWGNPENQFLCLTICGYKKPGMSEEDYKHHMTKVSAPMTKDLMVKYGVKRWTMIHNTTESRSFMNRLFDHQMCNLADFDCFSQVVFKDVDDYKRMKQDPWYKQHLIGDHEKFADTKKSMMTIGWITEFIRDGEVVDGMQNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8K9</accession>
    <name>A0A9W8Y8K9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pre-mRNA-processing factor 39</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_005163</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PRP39 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8K9"/>
    <dbReference type="OrthoDB" id="10265668at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030627">
        <property type="term" value="F:pre-mRNA 5'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000451">
        <property type="entry name" value="mRNA splicing protein (Prp39), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000064">
        <property type="entry name" value="Putative pre-mrna-processing factor 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059164">
        <property type="entry name" value="HAT_PRP39_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204">
        <property type="entry name" value="PRE-MRNA PROCESSING PROTEIN PRP39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204:SF5">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23241">
        <property type="entry name" value="HAT_PRP39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="536"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="536"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4370642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="563" mass="64987" checksum="87C05A2595BF811D" modified="2023-11-08" version="1">MLALGRGSIAQVVYSGDEAAVEIRKLLEAVVENEDDFEKWEALVTRATTLEGGVNRNSNPSAIDLVRNVFDCFLAKFPLFFGYWKKYADLEFSIGGTETAEEVYERGVSCITTSVDLWANYCTFKMDTSHDPDIIRQLFERGAHFVGRDFQSHPFWDKYIEFEERVQAPANVTKILSRVLYLPLYHIARYYEKFSALLGSRPLEELVDDETLDTLKNAVQLENQAGPEIAPLELERQLRLKLADYYYTAYSKTSQEVQTRWNYEQGIKRAYFHVTEVEDAELDNWRKYLDFEEKEGDFERTAFLYERCLVACALYPEFWLRYARWMFSQGKEENTRLIYMRASCIFVPISAPAVRLNWALFEEKLGRISVSRDIYIAMLEQAPGQTDTIISLAGVERRHEGNDAAVALLEQYIGQSDNQIGGILAAEQARILWHCKGAVDEARDIFKSKYARFLDSRDFWIKYLQFEVAQPSPDQKEAHARIKAVHELMRTKGTVSEEVSKELSHYYMAYLLNRGGKDAAEEYMQLDKIVNGYVSSDKSVPSQSLPQKRNGLPHHTASKRQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8W8</accession>
    <name>A0A9W8Y8W8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_004411</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Spindle pole body</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8W8"/>
    <dbReference type="OrthoDB" id="205662at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990498">
        <property type="term" value="C:mitotic spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099070">
        <property type="term" value="C:static microtubule bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061863">
        <property type="term" value="F:microtubule plus end polymerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030951">
        <property type="term" value="P:establishment or maintenance of microtubule cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990571">
        <property type="term" value="P:meiotic centromere clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000022">
        <property type="term" value="P:mitotic spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000019">
        <property type="entry name" value="Cytoskeleton-associated protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000282">
        <property type="entry name" value="Spindle pole body component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048492">
        <property type="entry name" value="Stu2_CTS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045110">
        <property type="entry name" value="XMAP215"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048491">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12609">
        <property type="entry name" value="MICROTUBULE ASSOCIATED PROTEIN XMAP215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21042">
        <property type="entry name" value="Stu2_CTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21041">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TOG" evidence="5">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="5">
        <location>
            <begin position="273"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="504"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="715"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="517"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="601"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="623"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="729"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="759"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="806"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4371195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="890" mass="96560" checksum="6F2FE10698B7BA46" modified="2023-11-08" version="1">MADQEEDFSALPLPDRFTHKNWKVRKEGYEAAAKEFSLAASESDAVVRQFVNDASIWKGVVGDSNVAAQQEGLAALCAFLDISGQAGCTRTRNITIATIAEKGLPSTRPAAKQKALEALMLYVETDKPDPVIEELLPILSHKQPKVIAATLDALTQIYHAYGCKTVEPKPVLKLLPKVYGHADKNVRAKAQELTVEFYRWLKEAMKPLFWNELKPVQQQDLDKLFEKVKDEPPPKQERLLRSQQAAKEAAVAAPGADDDLGDEEEEAAIDLEPEFEAVDVFPSIPKDYSEKLASSKWKDRKEVLDEVHKALNHPRIAEGPFDELVRGFAKSMKDANIAVVSTAANCVELLANGLKKSFARYRKDIMNAMMERLKEKKQSVTDAIGGALDAAFASTSFQECLEEILEFLKHKNPQVKLESSRFLIRCLKTSREAPTPEQAKAIAEAATKLLTESQEVQRSAGAEALGTLWKIMGDRIMNAHLDGLDDIRKNKIKEYFESAEVKAKYKPKAAAPPKPAAAPVATKKPLGKRPAPGGTKKPAPASTASSLAEDPVAPLAPKPTSRPGASKLGAPKSGLAAPSRLGGLKKPGSGLAAPSPRRPVASPPPEEAAPPPQPKFGAGRGLAGRPLGKPATEPAVAPATVSQAPTGLNLVERAELEELRAEVDRVRRQNEDLRGERTKLTSQIHELQNQNAQLIEDHTRDVLSIKAKETQLVRARSDYEAAEQTVQNQRREVDRLKRELSRQVRATSPPPTDLADQIYTDNNNPNSNGTRLGDSGYGSQGLYGRRDRAFVSGPTSPGGDGKENFDSLSRPGSNLSGKLSPLRADASGKSSLSSRGGGRISPAENGASRIGPSQAASGAESWKRAAEVTQNLKQRIEMMKAKQGIGRPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y992</accession>
    <name>A0A9W8Y992_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pre-rRNA processing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RRP9</name>
        <name evidence="7" type="ORF">N0V83_005297</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y992"/>
    <dbReference type="OrthoDB" id="189968at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039241">
        <property type="entry name" value="Rrp9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19865">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19865:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="221"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="263"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="364"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="55"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="370"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4370775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="601" mass="66507" checksum="F21D1EFD8D9464D7" modified="2023-11-08" version="1">MSSSFFTTPASQRKRKRTDGSSNVPKRRNTDTNSTRPARAPRRAEREESISGSDISDDDRGDVFDDVNDDSETSEDENETAAEKRLRLAERYLENIRSEVQEEVGFDAEQIDKDLIAERLKEDVAETKGKIYRSIAEELDFEEASHYCGFSGLQKSITGCAVKLPYAWTISKDLVVEKWEIADPKSYAPDADRPSNVTPRRTPKRLFWRKGNKNKKGERGYLGHTGEIISIAVSDSGKYLATGDKHARLIIWDADTLTPRHLFTRHRDAVMSLSFRRGTEQLFSGGADRAVIVWSAPESAYIETLVGHQDVVVGVAGGLEVNQETCVSVGARDRTARLWRVVEENQLVFQGGGTARQKGMDKLRKGRFGKNVEDTDKVKDDQVNGDHPEDDDPPIAYAEGSIDCVALLDASLFVTASDNGALSLWSVQRKKPLFTYPLTHGRDPRLTPEQMSANADAATSVKPGPRLPRYVTALATVPFADLILTASWDGWIRAWKIGADKRTIEPVGKVGRVPVNDEEESVNRDGAEDAILIRGIVNGLSVQERGDRGKDGLCIVAAVGREPRLARWMSGKVKNGIYVFEVPRKSLVNGTGDESGAEEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9D2</accession>
    <name>A0A9W8Y9D2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_004368</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9D2"/>
    <dbReference type="OrthoDB" id="5335493at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38790">
        <property type="entry name" value="2EXR DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38790:SF9">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4371152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="490" mass="56499" checksum="53834A1B31365A20" modified="2023-11-08" version="1">MYPTKISERTEGCPAQPEDSVTMASSSDVVLHAFHDFIQEERMRSFKFLAQHSHRYMQQNELLVASPAEADHINLVRERVNPRKTAGYDVQYYTFTQPLALGSLQRTVAFLVPYAVKCSIHSMKVVDLLDEVLSYVTPATTWFHNTYDWDTEHGMQSPDVQNDEQRMWWDNNGKTFRLLDLPPELRETVYLHTIGCIMAPITHTKRSTGNTNVYLALGISTGPKTRAGRYRDPDIQRPNMSILQLNKQIHKEATEVAYRDTTKRICTLGRHGKSPIRGFGPRLLALPYLLYFLPREAPHPAFLRHIQLEMSARTYFNFVGIQARKNQPLHSRRIRIPFGITSLRTFPALRSLDFRFVSPKHPDAACPWSSNQTRQNPEVLHHSCQKLWIDWFFVFAWDALIELNDKRRQGVKNSTKPIRSSLSGCVKDSTRKHWQSIFDDECMADYTDSIKAGVLQIRRDKTDTESIPCHCSTPCSAPWAEESRESLVEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9J5</accession>
    <name>A0A9W8Y9J5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Mannosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MNN4_1</name>
        <name evidence="7" type="ORF">N0V83_005350</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9J5"/>
    <dbReference type="OrthoDB" id="444255at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="6">
        <location>
            <begin position="10"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="6">
        <location>
            <begin position="120"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="189"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4369588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="217" mass="25701" checksum="C562F16AC0ADA1D1" modified="2023-11-08" version="1">MVRAYLNFFRENGLDTWIAHGTLLGWWWNGKRLPWDYDLDTQVSDATLQYLGANHNMTIHNYTSSDGNTTREFLIDVNPWIVERVRGNGMNIIDARWIDVRNGLFIDITGLSEVHPHNQPGVWSCKNYHYYKTDELYPMRETMFEGVMAKVPYNYDKILTDEYSENALILTEFNGHNWNRDLKAWVKNQDTMKREDEEREAKEEEDKKKALEQAKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9R3</accession>
    <name>A0A9W8Y9R3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9R3"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="718"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="782" mass="86870" checksum="D83225E8945C3F3D" modified="2023-11-08" version="1">MNNAMSSFQVFACIVATIIILGKGDAFVLGTAIAMAIFLFNLQYLNAAHPEAWIQHLLCVLARFCASAADSFKDTTDGARARQQLLLFPHGRQTALVALNIDRSKCLWDFNTVFRHAVIDSYYARLWKAMHQQPIHRQLLARFSTDVILLHVPSQIVVRSNNDESWSSDVPDSVLDDLASKTSSPPLETSKIKDTSDDDHTTSTNPEEERGRTPEDGKDATTYRDSVLLPGFTDTEEELAEDNAADANTDTASGHQHGSEEEAVNEATSSEAADPKEQAGKDPSATDPKGLQDTDDKDINELETALSLLSVCTVSAEEQSADERATSTDLQDPSGAEETVVNEDDTNKVETGFILLSTTKTEEQYIEEGATDSKHEDLSGAEKTAVDEHVSATDKTKAVTDDAPEEDEDVEYEIDANSSRELVPWTDPTKSTRGGDAPSGASSTFGPSPPRNTSSSPPLTGEAAAVQFWQSLGPVDQAYYAHYLRLYNTHEGQYTVRKAHWDRLVAGYPFGNPALGRKYPTWESCVADFTMIERILPFFYGVTHHWNVFKEYKISEAAFEFYKNAVKEAIVPEIKGLLTWDEQFAKREAQREDYTKRVVLVVGEDEDAENGVEVTQVGSPILQPPDLKVHCSHNLATATATSLKPQFMSDWHFEGDKLDPVIAEAAKKLGVKLPKITSKAELERLQKALEVPQGESIEAYKEMGWDNVLLLFGRHTEPIEEDEDESTHESASQNDSTDDQDENDEMKNDMDIDWRATDDMHGLKRWRVSYRQEDNTLLVQAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9T0</accession>
    <name>A0A9W8Y9T0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_004508</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9T0"/>
    <dbReference type="OrthoDB" id="2190219at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004712">
        <property type="entry name" value="Na+/H+_antiporter_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382:SF2">
        <property type="entry name" value="CATION_H+ EXCHANGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="338"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="374"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="423"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="7">
        <location>
            <begin position="39"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="476"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="484"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4371291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="505" mass="55771" checksum="7499768404D168B2" modified="2023-11-08" version="1">MQPRDTQSLSDETSSMAWSQLEPTGPHLAYLVLTSFLLIYALFSLMIRNRLHLSEPPLATAFGIAIGPRGANVVHPYDWGFNDGIIQETTRIIVGIQCFAVGLELPNGYFAKKWKALLVLLGPVMTFGWLICALFIMVLFETDFPTALTISACLTPTDPVLAASVLSNSQFSTRVPKRIRDLLSAESGCNDGVSFPFLFFGLSLLTRSTAAGVLKKWFLITVLYQCVVGVIIGLVLGHVFNILYKFSHTREMMGRASYLAFYLLLAIFSIGVASTLGVDDFLVAFCAGRGFSQGGNSPTADTRLPVVIDLMLNSTLFIFFGAMIPWQSFDKLDSITPLRLIGVLALILLLRRIPIVFTFYKLKMMPHVKTTTEALFVGHFGPMGVGALFLAIEARAQLETNTSLPLPHPPEHLSPERRRTVTLIWPIVCFVVFGSTIVHGLSTLLISVGGHFSRAEGERAPLIGGERDGLEHMVFDEDETAENEGDHRDEEAEEREYRGYLQGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9X3</accession>
    <name>A0A9W8Y9X3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_004531</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Mycotoxin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9X3"/>
    <dbReference type="OrthoDB" id="1844152at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11041">
        <property type="entry name" value="CYP503A1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46206">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46206:SF1">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9 10" id="KW-0349">Heme</keyword>
    <keyword evidence="7 9 10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="9">
        <location>
            <position position="472"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4371314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="525" mass="59631" checksum="E4660298D2E4BA42" modified="2023-11-08" version="1">MSWALNIIYSIIAITAFILIKLIQLKFSPRPALFNELDSVHPSPEWCPPTFARNVTTSILWFREYTNEGYQKFSKALDRPFALLTPWVRGATVLVLPPSRIPLLTRPDKAKDGEWTNLHGLLETTQLTYVVDDANVYQNLVHFDVVRKHMAPSDMRRLATVTAKETDLAFRDIWGTSTSWKTIKGWDECGRIISRASQRFLIGLPLSRDETMLETSRLYANALLVGGAIINCFPPWIRWVVAPLIAIRARYFQARYVKMLVPVVEERIRQYEAGLYKESDEDDFLQRMIPMCAKDAEQLNAERIALRTASLLTPLIFAICYVFAHCVLDIYSSPDKVEILAGLEEECRRVSAEFGGLSSSASMDALKGIDSTIRESMRVSDVMITNIFRDVTAGEVDVGNGLRVGPGVRMLFPTQNIHMDPDNYDDPTRFDAFRFSRPFKDKKSLGDESGEQDLITTPTPTFMPWGYGRHACPGRYFAAQSMKQALSYLVLNYDVELVGPRPKRKALLNMMIPPVDAKIRIRRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAK2</accession>
    <name>A0A9W8YAK2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_004864</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-binding monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAK2"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050775">
        <property type="entry name" value="FAD-binding_Monooxygenases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43098:SF2">
        <property type="entry name" value="FAD-BINDING MONOOXYGENASE AUSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43098">
        <property type="entry name" value="L-ORNITHINE N(5)-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4371644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="662" mass="74207" checksum="17BE5CB96643C4E8" modified="2023-11-08" version="1">MAQITTSPAGAIPSTQALEAGAAGSPSLASVQQKYEQERAKRLRTEGAKQFVDFYALKRLERFHKDPWLDENSSPPADGDRTEILILGAGYGGLLFAVRLLEAGFKLEDIRFADSANGFGGTWYWNRYPGLKCDIESYIYMPLLEETGYMPTEKYCTGLELQEHADRIAKKWNLTDRAWFQRTVDKMEWDERANEWVTHYTHRGPDGKDFPRKTMRSRFVVLTTGFTLVPRVPQIPGIGDFKGEMFHTARWDYKCTGGSPTDPNMVNLKDKSVGIIGTGATSIQVVPALANSVKTLYVFQRTPSAVDARDNSETDTEWWQREHSKPGWQKERRDNFNAFLANKRTKPSINLVGDGWTTMPSYSVLVGNPEAADMVKEDFSAVPAYVAKLHALDLSRQEKIRKRVEETVKNAAVAEQLKPWYPGWCKRPCFHDDYLEAFNNPNVNLVDTDGQGVELITENGIFFDGREYKIDILILSTGYRSPFNYSPGGRVNIDIIGRNGASLDKKWVNAVTTLHGMMSHDFPNLCWPGLSQSGGTPNFTHCMDHSAQHIAEILHNAVLETKQSGEGQSGYKYNFIVEPTAESEEEWSNAVASQAGVFAASAGCTPSYINAEGDLDKEVSEEVQRKRARGALWGKGFDDYCRILSEWRSKGDFSGLEIKEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAT2</accession>
    <name>A0A9W8YAT2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_003239</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAT2"/>
    <dbReference type="OrthoDB" id="3365399at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140115">
        <property type="term" value="P:export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042908">
        <property type="term" value="P:xenobiotic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF12">
        <property type="entry name" value="MULTIDRUG TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G06440)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="352"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="428"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="456"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="490"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="524"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="114"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="567"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="575"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4372948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="597" mass="65857" checksum="3995DD47603E6BF8" modified="2023-11-08" version="1">MVPNATDKDIGVASPSDRERVASTSARSNTSKRSGRIEDIETEDDDISSISTDSINSVVDRRPVIHQTWSRNTGQSWPGDKELAGITTVTTNATHDPRFELEFDDNGENPQDWSMAKKAMVIFFMSFSTLVVVMYSTSYTSGIPGMMRTFGIESKTLVILGVTTYLLGLAIGSLLLAPLSEMYGRRPVYLVAVFAFTVLIIPCALSNNLAQILVMRFFGAIAGSAMISNAPGTVSDIANEHYRALAFSIWSIGPMNGPVVGPLVGGFVYQALDWRWTNWVVMIGSGTSWFMVFLLKETYAPAILRAKAAKKRTETGDSRWHSRYDDKKAFWPLLRENLYRPLYMSVKEPICIFWNVYIALVYGVLYLCFVSYPIVFGEIRGWSPGMIGLGYMGIGIGGMTTIACEPLIRRMINAHKKDPETGKPYPEAMVSVVCIAAVCVPVGELIFAFTGGNPKIPWIAPLLAGIPFGAGNCGVFIYASNYLVHSYGIYAASALAGNAVLRSVMGGALPLAGPQMYATLGPQWSATMLSLIEFALIPIPVVFYLYGYKIRMKSDLIRQMREDREKLEGRKRRAAEREARLKPAEGMRLDEKKTLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAV8</accession>
    <name>A0A9W8YAV8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Flavin carrier protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">FLC3</name>
        <name evidence="11" type="ORF">N0V83_003284</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transient receptor potential (TRP) ion channel family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAV8"/>
    <dbReference type="OrthoDB" id="5212126at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010308">
        <property type="entry name" value="TRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040241">
        <property type="entry name" value="TRP_Flc/Pkd2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032800">
        <property type="entry name" value="TRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145:SF2">
        <property type="entry name" value="FLAVIN CARRIER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_7G01610)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06011">
        <property type="entry name" value="TRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14558">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01320">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040737910" evidence="9">
        <location>
            <begin position="23"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="333"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="411"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="503"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="532"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="565"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="ML-like" evidence="10">
        <location>
            <begin position="24"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="624"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="710"/>
            <end position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4372993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="731" mass="80181" checksum="589BA6FFBA63F5BE" modified="2023-11-08" version="1">MRPSLSKSLLWLAAAAAPLASAERFIESKSLNPCMSNSSFSATLFNVVFTPGNRSLGFNIEGISNIAGKVEAELELLVYGYSAMNDKIDPCDQKELDGMCPMNAGPLTIKSNFDISDDVIKSLPGIAYTIPDLDALVRIKIKDKSTNVQLACVEAELSNGQSVDQKAVAWTTAIIAGLGLVASAITSGLGHSNTAAHVAANAMSLFGYFQAQAFIGLCAVPLPPIVAAWTQNFQWTMGIIRVGFLQKMATWYQRATGGTPTTHLSQLSYVSVEVQKMKKMRRAIDFTAGAVGRLMARSNAAATEAQAGNERVVLHGIERVGFKARIETTNIFFTGYTFFVIFVIFTVIGVVAFKFICEGLTKAGKMKSDKFLDFRNGWQTVLKGILFRVVLIGFPQMMVLGFWELTERDSAGLVVLAILSMLTMIGIVAWASSKVVRIAQRSIAMHKNPAYILYSDPVALNKWGFLYVQFKAAAYWFIIPWLGYLLLKAMFVGLSQNSNRTQAIALLIIELALLVGVSWLRPWMDKKTNGFNIAIAAVNFLSAFFLFMFTEIFNQPALVTGVMGVIFFVINAAFSLVLLLMLLVSSGFALFTKNPDTRYQPMRDDRGSFIKSQTQLTTELDALGATARGEGKGTHPYTQSQSRIEDDDDFSSAEEQRQMAAKEGGFSEHYQPAPPRSQGGDGYTTSPSPPSFYDRRSPAPSYGRPESTRNDVQYRQGNNASPWQRGAGYEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB26</accession>
    <name>A0A9W8YB26_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Golgi apparatus membrane protein TVP38</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Golgi apparatus membrane protein tvp38</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TVP38_1</name>
        <name evidence="13" type="ORF">N0V83_004179</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Golgi membrane protein involved in vesicular trafficking and spindle migration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TVP38/TMEM64 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB26"/>
    <dbReference type="OrthoDB" id="166803at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000022">
        <property type="term" value="P:mitotic spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051076">
        <property type="entry name" value="Golgi_membrane_TVP38/TMEM64"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47549:SF1">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47549">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP38-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="12">
        <location>
            <begin position="134"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="336"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="345"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="360"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4372405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="380" mass="42017" checksum="2C240B1A76104AB0" modified="2023-11-08" version="1">MPASDYDARALALPLEDEETGETPVWSRRSTSSFRRNPSHQSHQPSWHQRVMDNYNKFNNRVTKQYNKLTPVQKVLFVIGNITLAVLGILFLVYNEKIFGALLPVAKKWRDVTAGWLILWALIFVVSFPPLIGYSTLLTLSGFVYGFPNGWFIAATATIAGSTASFLLSRTLLKSLVHRLLANDTRFAALALTLKHDGLKLLVMIRMCPLPYSLSNGAVATFPTVHWASYAAATAIVTPKLMLHIFIGSQLEKIAESGGKMDAKTKALSYVSIGIGAVAGVATGWFMYRKTKQRAAQLEAEERAGVRRVSIDDLENEYADDPGALEAAERLREEADDISLRTTWDDEYSDEPPETGDAVEIGDDPFIDGDASDEEGRGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB54</accession>
    <name>A0A9W8YB54_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_003286</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB54"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05283">
        <property type="entry name" value="CAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000022">
        <property type="entry name" value="Cinnamyl alcohol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000022">
        <property type="entry name" value="NADP-dependent alcohol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047109">
        <property type="entry name" value="CAD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42683">
        <property type="entry name" value="ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="6">
        <location>
            <begin position="9"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4372995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="331" mass="35907" checksum="78DD7E21DABDE8D0" modified="2023-11-08" version="1">MPSFTVYKGSKEGKIVKSETKKPDLTDDQVLVKVTASGLCGTDEHYKSADMVLGHEGAGVVEHVGPAVKSLKKGDRVGWGYEHNSCGACDSCLSGRETYCPEREMYGEANLDQGSFASHAVWREAFLFRIPDELTDAEAAPLMCGGATVFNALHTYGIKSTDRVGVMGVGGLGHLAIQFASKMGCEVVVLSGSDSKEQEAKKLGAKEFIAMKGKKELSLERKLDALLVTASVNPDWSLLLPIMAPNSTIFPMTVAFDNFEIPHLPFIQKGITIQGSIVAARAVHRKMLAFSAFHGIKPMVMEFPMSEKGIEEAMSTLRDGNMRYRGVLKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBG2</accession>
    <name>A0A9W8YBG2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Phosphate metabolism protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PHM7</name>
        <name evidence="13" type="ORF">N0V83_003320</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBG2"/>
    <dbReference type="OrthoDB" id="1076608at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022257">
        <property type="entry name" value="PHM7_ext"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF26">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G10920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12621">
        <property type="entry name" value="PHM7_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="394"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="441"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="486"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="538"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="597"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="648"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="681"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="11">
        <location>
            <begin position="23"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="12">
        <location>
            <begin position="198"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="9">
        <location>
            <begin position="393"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="10TM putative phosphate transporter extracellular tail" evidence="10">
        <location>
            <begin position="763"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="720"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="726"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4373029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="869" mass="96679" checksum="AEC5D45DBD621C8E" modified="2023-11-08" version="1">MSTTTAGQTGSAKKQSSSSLSAMISTLVPTVLLAAAFVTAFLIARKYFRRVYAPRTYLNHLGQQRQTPAQGGGFLGWIKGFAKIKDAYILDHQSIDGYLFVRFFKVLIVTSFLGCLITWPVLFPVNATGGAGQTQLDLLSMSNISTDGKNINRYYAHALISVVFLMLVLVIIARESFYTVNLRQAYRRSPWGASRLSSRTILFTNVPKTLSQTALFEMFPGVKHAWVASNTKDLDELVEDRDKTAMKLEAGEVQLSRDANMNRIKADKGKKHFVASDVSDGTKWIDPKKRPTHKLKFLIGKKVDTIEYGRSHLAELLPKITQEQDKHWNGQGDLVGAVFLEFDTQRHAQDAWQVMQKRKTKPNKKLQARQLGVMPQEVVWGNLRIKPAEHLVRWAIATAFITVMIIFFAIPVSFVGLISNINYLASRFSWLSWILKIPKVILGVITGLLPSVLLAVLMALVPIVCRLMAKLAGYVTYSQIELKTQSWYFAFQVVQVFLVATLSSAITSVINDVLNNPGIVLKLLAQNLPKASNFYTSYFLLLGFSFAASTLLNIGGFVVVVLLGRILPGKTPRKIFDKLTKLSSPSWGSEFPKWTNLAVIAITYSGIAPLILGFAVLCFSLIYIAFKYNFLYVYETNIDTKGEAYQKALGQLLTGVYLSEICLLGLFAIATADNIYAVGPLAIMAVLLALTILFHITLGAALRHEEARLAYTDPAGFNGHHTATNGHHEDGLHGEKAATQDSTASNMAPKASRMPLFLRKIFNPERHSIHTLSSQLDQFYHTPQEPLPIEIQKRAFFNPSVTSPAPVLWIVKDDMGISAREIRDTQKAVPGLHVTDEQATFNEKGKVYWQGAEDGMSREAPVFDERIVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBR8</accession>
    <name>A0A9W8YBR8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tyrosine specific protein phosphatases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003378</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBR8"/>
    <dbReference type="OrthoDB" id="449382at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026893">
        <property type="entry name" value="Tyr/Ser_Pase_IphP-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF1">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13350">
        <property type="entry name" value="Y_phosphatase3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="2">
        <location>
            <begin position="173"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="334" mass="37386" checksum="2AE7B5C56A66BD8F" modified="2023-11-08" version="1">MLTAKPAEASPPTSSEPAPRAPHPSFFPFHIVPGVSNFRDIGGWPITLPSIPEKPHHVRRGILFRGSDTTRITAAGIAKLQELHVKTDFDLRSKQQIEKAGGFKDMQEWGIERVWAPVFSDEEYTEEKARERYEMYASEDPANTQIGTEAHAYPQDIVSAFIEILTSGAPMMKTVLQHLLSTTIEQEPQPPPALFMHCTTGNNRTGIFISLLLLLLHVPAPTIIHEYTLSELGLAPTRHINVDRLLAKGAFKEYGEREARRRCERMVGARAGSMEALLDEVERRWGGAEGYFRGCGVERGEVGRVREVFSVQGMGIRELFGEGAQLSRKRRYPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCG3</accession>
    <name>A0A9W8YCG3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_003512</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCG3"/>
    <dbReference type="OrthoDB" id="6428174at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF85">
        <property type="entry name" value="DUF803 DOMAIN MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_1G15880)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="369"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="380"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="410"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4373219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="421" mass="46136" checksum="AE36CB1806A87AB0" modified="2023-11-08" version="1">MVEDKYIGLMLAMSSSLAIGASFVITKKGLNASMEKHGFNGDGFGYLQNPVWWAGITTMVLGEIFNFAAYAFAPAILVTPLGALSVLIGAVLGSYFLDEQLGLLGKIGCAICLIGSVIIVLHAPPDKEVSSVEEILHLALQPGFLFYCAFVAVFSVFMIYKIAPKYGRKNPLIYLSICSTTGSVSIMFIKAFGIALKMTFAGNNQFTHPSTYVFVIMVVGCILTQMNYFNKALSQFSTNIVNPLYYVTFTTCTLVASCLLFQGFNTTSAVNTISLLCGFLIIFSGVYLLNLSREDPNGNRHLGSNFTDGPPSDAISGFPTRRSMQARRSEELISERYTNGAAREGRQSYADRGDRSALMHDYDVENQFELGDLAEDSEDESRNTKRTSFDEDSRLNGRLSGTGRGRVQLESVQPKRNSSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCH3</accession>
    <name>A0A9W8YCH3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ASST-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCH3"/>
    <dbReference type="OrthoDB" id="5427350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053143">
        <property type="entry name" value="Arylsulfate_ST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039535">
        <property type="entry name" value="ASST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35340:SF5">
        <property type="entry name" value="ASST-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35340">
        <property type="entry name" value="PQQ ENZYME REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14269">
        <property type="entry name" value="Arylsulfotran_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="416"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="425"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="487" mass="53715" checksum="B5EE9157BEFDEFB2" modified="2023-11-08" version="1">MRLGTLVWTGNYTEPFDFRTQTYKGQPVLTFWSGELENGYGRGSYYILNQSYAEIAHFQVNHFGDNMGDIHEFTITSDDTALVPIYRPITWDLTSSGGPQDGWIFENTIQEINIETGALVFEWNASTHVGINESYNALTDDVGRAEDAPWDYFHINTISKDKNGDYLVSARVMDCVYKISGTDGKIIWRLHGKQSDFDVDSAADFAFQHDAKWVDAEQTRMTLFDNGPTDSVQYSRGLLLDVNQDDKTVKLVREFANGAKTFAQFEGCLQAIDPSNATTNFLLGFGSQQYFAELDHDGNILLDVQFGKTNTVNSYRAFKQQWQGKPLTKPDVHWDKTGNKSYFSWNGATDVETWEVYTANTSDSKAWKNVTSTRRTGFETTVDLGNMQLNTYVRGRALNTTGGVLGWTRASDGNQLYDAPDDVETSSSSSSSSVSSTSTSTSSSSKTSAAPSSSSSTAAAAAARATQALYEQVYVAAVVVVGGLAFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD88</accession>
    <name>A0A9W8YD88_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_003452</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD88"/>
    <dbReference type="OrthoDB" id="310217at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF98">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="46"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="427"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="578"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="432"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4373161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="897" mass="100697" checksum="FC482F25BF2B88E7" modified="2023-11-08" version="1">MHLLRRATRYLKNHGKALGNRMLGKSSQANTNASNSLQCSNFHLRYAPVRLLEGGGNGTVHLYRNTNKGTLVAVKTIYHDDPQSPPNEVRILQLLGGSHPHIVQYHTMLSHPALDFHVQLVFEYCQFGDLVDYVNSSIGRDRDVPEPFIWHVFKHVAAGLSFMHLNSVVHGDIKSANILMAAPRDGEIYPLPRIADFGTAQVNPPYNVPYGHHATMGWQPPEWEHCHGPAADVWGLGSIVHELAHKCLPQRAIEPPPAMDSMTWFESSGKTVPPGTERVVWYKQFCFYMAFHPAAAMRLDRPRPSSSTPAPVVYSRLLNYMVMRTLDTDADRRISTIELTSLLPVLDTVARNLRGLEQEAILSDFDDGRDGEWKEVSFVTDSQVLRQLFCTVASQAKHEGNMEMLSWGASLVKLMNPRDQPEALLNPESIAKHKMGTNRKKKSGGGRGGRKTASGGGTKAATATPEPSMPKLKLKLKLPSNPNSGTESDDAPTDWNALGRPKEFEEEEEELPVAEQTVTTRRGRQVRKPQTGDDIYGSDFDRLLEQSSDQKVDDDDDYHYEAGADDVQVFQVQATRKSGRPSKNVSFAPEEDLDDGEQVGMSSDTITRSSPQSGLFLIPPDPEVYMLIEGLCGTSEIFINLFPVEETATPGVEKPYTAKMLIQLYLVGYENKHWNVCDLVADTWIRAFHQRRERAERDTQQSIWRRNKVLENRKRELEEAARKIERIGGKAPKGIGFDPHPPDYDLPVNDPLLEADVTLFNPALLTELYKHTPKLCGARLLWADAMTLCGAKMEQEIVDSAKNGTEWPADLLFDIMQTSLRMLGKKLTLKIEESTEGCWCKRYHEHVKHGLPCYRELAWLQVNEEDDGDEQEGGDGDDMDFESAMEAELEKSFAMDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDD2</accession>
    <name>A0A9W8YDD2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Malic acid transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_004017</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDD2"/>
    <dbReference type="OrthoDB" id="2901184at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015140">
        <property type="term" value="F:malate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09317">
        <property type="entry name" value="TDT_Mae1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.150">
        <property type="entry name" value="Voltage-dependent anion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030185">
        <property type="entry name" value="Mae1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004695">
        <property type="entry name" value="SLAC1/Mae1/Ssu1/TehA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038665">
        <property type="entry name" value="Voltage-dep_anion_channel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31162:SF0">
        <property type="entry name" value="MALIC ACID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31162">
        <property type="entry name" value="MALIC ACID TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03595">
        <property type="entry name" value="SLAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="406"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="466"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="85"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4372243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="510" mass="57021" checksum="3EE07CD8880E8064" modified="2023-11-08" version="1">MQYGPPLGFRASSPKQMLDYAQPVESYSPLSDLQQQTQHQSHQDSPTPGSRSGQTTRVQTPADADPSKDDYFKDPEYNNGYPLDTKLKRSKPEPPSKEPLSHSHSPIDTQSIDLEKYEKVKEWDKHTKLPGRKDPDQPYLPYGQRLKHFTWAWYTLTMATGGIATLISIQPHAFPGLITIGAIFYVINLIFFTGITMTMILRFTKFPGALKGSISHEREGLFLGPFFLSIATIITGTQKYVIEAYEVDHPNRTWAITSIAVAFWGYTFAAFCLATFQYSFLFCCHTYKLTSFMPSWLLPIFPVMLSGTIASVIAKDQPVASRMPIIVAGLGCQGLGFTVAILMYAHYIGRLMQVGYPDREHRGAMFIGVGPPSFTCLALIGMANALPDEFDLQGDGLVDAKLLRTMALVVALFLWVLAMWFFLITLVAVINSWAVYFHLGWWAMVFPNTGFVIATISIGNSLQDETILYVGNGLSIAIVVMWLFVIFNHVRAVYVADIMYPGLDEDIADH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDH8</accession>
    <name>A0A9W8YDH8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">60S ribosomal subunit assembly or modification protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SQT1</name>
        <name evidence="5" type="ORF">N0V83_001881</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDH8"/>
    <dbReference type="OrthoDB" id="10261640at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051179">
        <property type="entry name" value="WD_repeat_multifunction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857:SF8">
        <property type="entry name" value="ANGIO-ASSOCIATED MIGRATORY CELL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="130"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="226"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="330"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="12"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="334"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4374804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="511" mass="53690" checksum="06C9B7FC17192FE6" modified="2023-11-08" version="1">MAIPNPNQPHQEEDDIDYHDEDNTLDADEAEEILEEDDDVPMDSDDEDEEGGRDFQMEINLQNDSIAHFDEHKDSIFCIAQHPVHSEIIATGGGDDIGYVIDISPAAAATQPSNGAEPTEREGLKSLFKLDGHKDSINAITFTEPKGQFVATSGLDGKLRAWQGVPDGKKWKFLAEVQEVEEINWLTPNPSPDHPNVVALGANDGSVWVYQLSTDKGAELQVLQAYYLHTESCTGGTWSPDGSLLATISEDSSLYVWDVFGDAASQGLTSTSDSQTVVGLTGLDERFRVEGGLYSVGIAPSGAFVAVGGPEGQIRIIGLPRLSLAAPAQASTSSGGGGAKNKAGGGKQAAAKGGASSAGQAGQILASLQAGSDNVECLSFSSPPLTLMAAGNVDGSITLFDTAHRFAVRRRIEDAHADEEYPQAVVKLDFVRQEGAGGWLLTSAGYDGVLRRWDTRGGTAAAGKGLVGEWKGHRGGGEGGGIMGFVQGNGEAVVTAGDDHVALVFKTPLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDS8</accession>
    <name>A0A9W8YDS8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1479-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_002502</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDS8"/>
    <dbReference type="OrthoDB" id="8249012at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010856">
        <property type="entry name" value="Gig2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30613:SF1">
        <property type="entry name" value="DUF1479 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G09280)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30613">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YBIU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07350">
        <property type="entry name" value="Gig2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4373763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="440" mass="49665" checksum="DBF37CF985A7A370" modified="2023-11-08" version="1">MPGALIPGALHEWPAWNEYTFEKSEKGPLLTQYKKDIVARYGEEALRKSWLKVCAELEKLTDEISEKGSSIIPEIAYDDFFKLSAEEQEKIKSVGCFVVRQAVSEDLATEWFNDAKTYVADNKASVTGWPTETPFVLNIYWSPSQQAARTHPRQLKLQRAINTWWHDEEGNTSPEPLSYADGIRIRPPGVPFYGLGPHIDAGSLSRWVDPGYIKVYDSIFSGHPEDFDAFDLTTRKKANQSLFEGFAHSSVFRSFQGWTALSKAAPKEGSLLLYPNVKTAIAYVLLRPFFSQPKDEKDIMDATKWTFDVETPWFPGTWREDSQFASPTSHPHLRLKECMLHIPPMQAGDTIWWHCDMLHAVEVEHIGDHEASVVYVGAVPTTEVNTAYIKGQLQKFVDGGQTPHDFDHKKLAPESTFKGYLGEKGILGGDEGRKAFGFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDY6</accession>
    <name>A0A9W8YDY6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDY6"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF31">
        <property type="entry name" value="LAEA-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G01930)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="42552" checksum="1D3332581C8AE73B" modified="2023-11-08" version="1">MQQSRASRSPSKSPKTAAASPPKIKPEATTSSEAPPVDSGIGSSSEYDQDNSGNHLLEAQDDTSEASTDEGYAESDASSFLSSILSEVRRGVEIEGRLYPTYGKHAYGMPMDEKEMDRNDLQHHKYSLLLRDRLFVAPVPDAQLTSSNARILDLGTGTGIWAIDMADAYPSAELLGVDIAATQPPFVPPNCTFEIDDVEEDWPYRPAHFDFIHARDLMTAVRDWPRLLAQAYTHLKPGGWIQLASTIPGALTDDDSIPPNSGYVECGRLYFEIAEKMGAPLDAPRAWSSQMRGTGFQNVQDEVYKLPMGPWPRSKRLRTVGKLEQIMILEGGFEAYMLRGYTQVLGGRKEDLQVVMALARREIRDPGVHTYVQFHVTYGQKPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDZ8</accession>
    <name>A0A9W8YDZ8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_003102</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDZ8"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051061">
        <property type="entry name" value="Zinc_finger_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179:SF13">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="290"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="322"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="79"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="177"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="224"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="351"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="444"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="80"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="224"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="451"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="501"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4374361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="636" mass="70433" checksum="B9F4925F89A7D5E5" modified="2023-11-08" version="1">MVDYVPYPSNIPQDFELYSNTHEQQMEFHNSQAFLPSSTYGMEQTFSAPYDPMGSLADAPRAQDLQFHYDAIAQGVKPFQYQSPTGSPNSSAHSFHDQPPVLSASSESGASVSSSAMHSPSLAPQFNEPWNPMGLGLTSSFEYPGIAAVEKTFVDPNLIQPFAFAPHSPFPEIRTTPSPYFPVIEQPPSPALSHISSHSQRPGSARIKRGSASPYLHTQQYQPYPQFSGQRRGSVNSLHSQHSVTSRKSGSSYELDDDTREKGLCPLPECGRVFKDLKAHMLTHQNERPEKCPITTCEYNIKGFARKYDKNRHTLTHYKGTMVCGFCPGSGSAAEKSFNRADVFKRHLTSVHGVEQNPPNSRKKSPSGRKAYSGQNNVAGTCSTCSVTFANAQEFYEHLDDCVLRVVQQADPSEAINQRLLTSVAEDKDVTETLDRNGLSKSIEYTIPSYNDEEEDDDDEEIDNDDQNDDTYGMRRSRSGKGGIKSTKGVGATQHVGGGNLPNTSNNKITKTQKGPRAGLTFSKGGVAISGSGRKKRKNYPVSWGCPTDKMKMKKRVLCVYDGSRRLWKDDMMLDQEFEVRMKLPDGKSYVTDLDVQTLNRAQALHGATAEEKGPWLPDNIMGAIEVPPHMEEFMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE53</accession>
    <name>A0A9W8YE53_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Carbohydrate kinase PfkB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_002323</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE53"/>
    <dbReference type="OrthoDB" id="198885at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004730">
        <property type="term" value="F:pseudouridylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01941">
        <property type="entry name" value="YeiC_kinase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1790.10">
        <property type="entry name" value="Indigoidine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01876">
        <property type="entry name" value="PsiMP_glycosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022830">
        <property type="entry name" value="Indigdn_synthA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007342">
        <property type="entry name" value="PsuG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42909:SF1">
        <property type="entry name" value="CARBOHYDRATE KINASE PFKB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42909">
        <property type="entry name" value="ZGC:136858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04227">
        <property type="entry name" value="Indigoidine_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110581">
        <property type="entry name" value="Indigoidine synthase A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00583">
        <property type="entry name" value="PFKB_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="9">
        <location>
            <begin position="432"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="9">
        <location>
            <begin position="740"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="379"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4375237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="797" mass="84343" checksum="E4686E9786E42B82" modified="2023-11-08" version="1">MTWLATRAVSRPRLPRACLGAAAALGRRCLTNNGFFRVSDEVREALHSNKPVVALETTIYTHGFPYPENVALASLLESVVRTNGGVPATIGILDGIARVGMNPEDIIRLASSAGKSNTLKLSRRDLSYVGGMRLTNKLFNGGTTIAGTMLLAHLAGIKIFGTGGLGGVHRGAESSMDISADLTELGRTPVTVVSSGCKSFLDIPRTIEYLETEGVGVGTFADGRKGNVDFPAFWSRDSGVKSPTTIADEVEAAAIIHAQHALQIKSGLLFANPVPSEFAIPKAEMDVIIEEALKQADAAGISGKDNTPFVLAKIKELSKGASIPANRALIESNVKRATIVARELAILQGKQAEAQGRDPYFTPVQFSPAATTEDRLSNASLSPSATPATPATSISESAADIDVVVAGALAVDFSCDYAPFTTSTSQTEPLPHTSNPAVINQTLGGVAHNIAKAVHLLGSPVRLHSAVGDDLSGRAAVSQLQEEGMLVSGIETLSKPARTAQYVAVNNANKDLALAMADMSILENIPEHTIEKLATTLFNNPSTRPKVFVADANWSSTSLYTWLQAARHTSTPTIFEPVSTAKSLRIFPPNSSSPTTAPNVFPTPLADIITPNTYELTALHNHAHTSSYFDSQAWFAIIDALGIPPTGLRVPLTYTTTSSLVDQGIPQQAIKLLPFFPTILTKLGPQGVILTKLLSVNAEELHDDAEREFVLSRSMSAEARELGVGGLYVRLFSPERVLAPGEVISVNGIGDTFCGAVAVGIAKGRRVQDIVGFAQKAAGLSLRCRESVSPELKGLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEP7</accession>
    <name>A0A9W8YEP7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Prohibitin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PHB2</name>
        <name evidence="10" type="ORF">N0V83_001131</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Prohibitin probably acts as a holdase/unfoldase for the stabilization of newly synthesized mitochondrial proteins. Involved in mitophagy. Required for the switch to necrotrophic growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">The mitochondrial prohibitin complex consists of two subunits (PHB1 and PHB2). The subunits assemble into a membrane-associated ring-shaped supercomplex of approximately 1 mDa. Interacts with ATG24/SNX4; the interaction is direct and plays a role in mitophagy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the prohibitin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEP7"/>
    <dbReference type="OrthoDB" id="275637at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035632">
        <property type="term" value="C:mitochondrial prohibitin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03401">
        <property type="entry name" value="SPFH_prohibitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000001">
        <property type="entry name" value="Prohibitin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000163">
        <property type="entry name" value="Prohibitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222">
        <property type="entry name" value="PROHIBITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222:SF1">
        <property type="entry name" value="PROHIBITIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00679">
        <property type="entry name" value="PROHIBITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="9">
        <location>
            <begin position="55"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4375853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="311" mass="34525" checksum="70374848154CA2B3" modified="2023-11-08" version="1">MSAPDPKEAWQRIQNELTRRSARFGGSGGGPPKGLFGGIGGLVLIGGGIWMANNALFNVDGGHRAIKYTRVGGVQKEIYNEGTHFRIPWFETPITYDVRAKPRNVASLTGTKDLQMVNITCRVLSRPRVEALPQIYRTLGTDYDERVLPSIVNEVLKSVVAQFNASQLITQRENVSRLVRDNLVRRAARFNIMLDDVSLTHLAFSPEFTAAVEAKQVAQQEAQRAAFVVDKARQEKQATVVRAQGEARSAELIGDAIKKSRSYVDLREFENARNIAQILQQSSNKVYLDSKGLGLDISQTTADKEQRASRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF76</accession>
    <name>A0A9W8YF76_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Class II myosin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">MYO1_1</name>
        <name evidence="13" type="ORF">N0V83_001242</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Binds to cdc4 and rlc1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120104">
        <property type="term" value="C:mitotic actomyosin contractile ring, proximal layer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902404">
        <property type="term" value="P:mitotic actomyosin contractile ring contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01377">
        <property type="entry name" value="MYSc_class_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000001">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000101">
        <property type="entry name" value="Slow myosin heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.270.10">
        <property type="entry name" value="Myosin, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055914">
        <property type="entry name" value="DUF7491"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF857">
        <property type="entry name" value="MYOSIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24319">
        <property type="entry name" value="DUF7491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 9" id="KW-0518">Myosin</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="12">
        <location>
            <begin position="166"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="51"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="9">
        <location>
            <begin position="739"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1659"/>
            <end position="1712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2364"/>
            <end position="2392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2479"/>
            <end position="2506"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="938"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1310"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1463"/>
            <end position="1581"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1607"/>
            <end position="1644"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1846"/>
            <end position="1929"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1958"/>
            <end position="2063"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="2099"/>
            <end position="2154"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="2190"/>
            <end position="2359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1660"/>
            <end position="1672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1688"/>
            <end position="1707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="2481"/>
            <end position="2499"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="259"/>
            <end position="266"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4375962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="2525" mass="288964" checksum="A306A4CBF40F71CE" modified="2023-11-08" version="1">MAAPFARNSRNSPFARNTPSPSPGLTPAALRPKSGLVTSPLAEAEPTMAHARNQSLTNLSSPLLGRSDSTRLRSNSHRNSTPVGTFAPQFISEDGDETPKRGGIVEGENDFSGKRYVWLRDPSTAFVRGWVVEELPAGRLLVQCDNGSQQEVDADAVDKVNPAKFDKADDMAELTHLNEPSVIHNLHMRYQADLIYTYSGLFLVTVNPYCPLPIYSREYINMYRGRSREETKPHIFAMADQAFRSLVDEGTNQSILVTGESGAGKTENTKKVIQYLAAVANSDDPRAKSGGRHLSNLSEQILRANPILEAFGNAQTVRNNNSSRFGKFIRIEFSRTGQIAGAYIDWYLLEKSRVVRLNGNERNYHVFYQLLRGADNKMKQDFFLANKDTEDFDYTKHGNDVISGVSDRDEWNALIEAFHIMGFTDKEQASILRTVAAVLHLGNIRAMKESRRADQASLNLEAEQQAQIVAQLLGISADDFIKALLHPRVKAGREWVEKVQTPEQVNLSIDALAKGIYERGFGDLVDRINRQLDQTGSGGDDSHFIGVLDIAGFEIFDENSFEQLCINYTNEKLQQFFNHHMFVLEQEEYAREQIEWKFIDFGRDLQPTIDLIELPNPIGIFSCLDEDSVMPKATDKSFTEKLHSLWDRKSTKYKRSLLTQGFMLTHYAAEVEYSTDGWLEKNKDPLNDNVTRLLSASTDKQVAHLFADCTDMDDEAGGSRSRVKKGLFRTVAQRHKEQLSSLMTQLHSTHPHFVRCILPNHKKRPKQFSAPLVLDQLRCNGVLEGIRIARTGFPNRLPFVEFRQRYEVLCKDLPKGYLEGQVAAKLILEKLNLDKALYRVGITKVFFRAGVLAELEDQRDTLIRDIMVRFQSAARGFVQRRLAHKRLYRAEATRIVQRNFQVYLDLQSNPWWRLFARMRPMLGATRQAAEVKKRDEMIHQLEEKMQHEAAEKQRVEDERRRAEMDIQRIQKTLESERALALDKEEIFKRLQLRETELAEKLAGAIEDQDQLEDQLDALMEAKKKSEDQVELLRNELEQAGQIISKLEGETQELERQASHLDQQLKELEALRSQRTQAEETLGQEVKMLKSHLSLKDRKLQDLEAKLLKTDQDLDIKLAASVKEVQAGKKQIKELLDENRQIRQQITDLSSTSTSYEDLIRRKESELAILKTDLKNYENDRHNFDQEKRTLASKHDDLQSRLREAKAELDAVRSQKQQLEREAADAKRLLEEKISEDAQSSEGRKLLEEQIEGLKTELFQVQKELSRERQSRDDVEMLGEHKYDTLKRDFDALNDSKITIEKEMYSQQDTLRRAVETRTAHEKERKEYQNELRTLREKYLKLEEEKLDADAAAERAQSKMALERQAIQQQEIEAKDEQLDQLEAERNDLAEQVHNLTQMLADSDSFKERNDQQKERLEREIVTIKGRLTASENDNRALLNKLQQKNLDIARSNSRAGDTQRSKITQLTHEKSKVEEDYKRLFRQLEDAQLTIVSLEKQKEKLSLSLEDLNHEVTREHKTSRNAEKASSTINLQLADVNRKLETERQLRTQAQANTRAVQATLDNSNRELDECRKQLVLLRKVFDPESADVAITYDAAKPDIARTVDIAERLEAANQALRVAAERYSRAETQLDELRERHEGELNDLDTRHANSKRALLEEMNSSQVNQANARRSPTHNRKDSENRRPFSITNTPTNQRHMSNATNNSARSDRTVDTVTYNNSMDMAAELELVQNQLQMSEMRNKHLQSQLERSPVRNGWQDDSPSVRRVQKLERENFRLHDMLDDSAKKVSVLEQSLRSGQLSYQEVQTKSHEELFDLLTSQEQSRKSIVQAHNVAINELADAKTAFDDIKQVKAVLETELRDIQSEFEDMIGEREQEQANHNQLLQEFADLQIRLDNETSSLLDTQSSLNLYKNRADEYFSKLEQAEIAVLKASRAEQFAKTQAREAEETCATIMAERKEMDSMVEDLQRTVQRYDERVEDLSADLQTALQAKKRLQNELEDYRSQHAHDIEDSESSLEQTRKKYQVELSTLTHELDLERENVIYVREENGRLREEIEELRNKWDDEVLNSSTWAKEKSRLEMTLQVISNSRDEAANAHNEAQSKIVSLLSQVRNLRTNVDDLAAERDSAIEEKKRLESRLQEAADKLDDLSRSESPAMRNAAAYDRDLLELKSGLAQQEDITAAAVGKMRRAEALAQELQKDIVAEREANVQLHKDKAAVEKGLKDLQLRLVDLETKGYSGSAGKDVRFLHGRIQELEQALETSESTRLTESRSVRNVDRTVKDLQNQIERRDKQNTSVLEDLNKSRDKISNLLATIDELQSSDSTNQLAAKRAERELREEKEKNLRLERELESWKTLRVERRSNLGVPGQQQQNGGSEAGGDNRSRRGSSIGLLMGEMGEVAASDKGKFGGSLRSMGLRRLGPHGQGEEGLVKQHLYRKPSVLDVVDEVVHIDLDDGDQDTLVDEGGEQGEKHVHVEITDDEAEDDDDSEADAEDSPAGEAAAGCGVKRKRTIRVIIRSRAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFA8</accession>
    <name>A0A9W8YFA8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_001287</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFA8"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033768">
        <property type="term" value="C:SUMO-targeted ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032183">
        <property type="term" value="F:SUMO binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140082">
        <property type="term" value="F:SUMO-ubiquitin ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049627">
        <property type="entry name" value="SLX8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47094">
        <property type="entry name" value="ELFLESS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47094:SF1">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="223"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="95"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="301"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4376007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="312" mass="34203" checksum="42451CBACEAC5DF5" modified="2023-11-08" version="1">MSSPDYSAYVSEYNSQPSHTAAARNSQSPLTGHDPNFLDQLLPPFNPSSHRASPYSLAEVHFDEAYRPSPSPSPPISNFLDTILNPAGLDHNRYSPFVYRPSPPRQSFAAASEMAPSTRARPQTSQRPARLPNGYVDLTATPHSPPRTRKRESPSPGPSAKRQKRGHAKTARRESAEPVNVEEVDLTDDKQPVQEILQKQREDAIKAQTKPEEKPTTFNTFTCVICMDNPTDLTATACGHLFCHTCLMEALIAGENRTGPGEPKRSQCPVCRKSISRSKATDIIPLLLKKGLATQPRKKAAASSTTTAPKVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFG6</accession>
    <name>A0A9W8YFG6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-N-acetylhexosaminidase</fullName>
            <ecNumber evidence="3">3.2.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_001378</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.</text>
            <dbReference type="EC" id="3.2.1.52"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.52" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFG6"/>
    <dbReference type="OrthoDB" id="428480at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004563">
        <property type="term" value="F:beta-N-acetylhexosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06564">
        <property type="entry name" value="GH20_DspB_LnbB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000055">
        <property type="entry name" value="Uracil-5-carboxylate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.379.10">
        <property type="entry name" value="Chitobiase/beta-hexosaminidase domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025705">
        <property type="entry name" value="Beta_hexosaminidase_sua/sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052764">
        <property type="entry name" value="GH20_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015883">
        <property type="entry name" value="Glyco_hydro_20_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029018">
        <property type="entry name" value="Hex-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015882">
        <property type="entry name" value="HEX_bac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43678:SF1">
        <property type="entry name" value="BETA-N-ACETYLHEXOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43678">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_2G00640)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00728">
        <property type="entry name" value="Glyco_hydro_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02838">
        <property type="entry name" value="Glyco_hydro_20b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00738">
        <property type="entry name" value="GLHYDRLASE20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55545">
        <property type="entry name" value="beta-N-acetylhexosaminidase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1071"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="domain" description="Amidohydrolase-related" evidence="11">
        <location>
            <begin position="94"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-hexosaminidase bacterial type N-terminal" evidence="10">
        <location>
            <begin position="401"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 20 catalytic" evidence="9">
        <location>
            <begin position="500"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="855"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="864"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR625705-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4376097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1091" mass="120005" checksum="13CD0C271ABA8452" modified="2023-11-08" version="1">MAIVDIHTHVYPPKYMELLKSRSTVPYVRTFPDAPDSARLIILPGEDDPSTPSTSRGRPIGSEYYDIKEKIAFMDLHKINKSVISLANPWLDFLSVEEAGEAAKKINDDVNDQCSQYPGRLYFFGTLPLSASPDVITAEIERLSTLKYARGVIMGTSGLGQGLDDAKLDPVYAALEKHQQLIFLHPHYGLPSSVYGPRASEYGHVLPLALGFPLETTIAVTRMMLSGVWDKFTKLNVLLAHSGGTLPFLAGRIESCILHDGHLEKHGKTEKRRDVWDILKTNIYLDAVIYSEVGLKAALEASGSDRLLFGTDHPFFPPLEEDATEWHSVNANYGAISKAFAGDDKKAQDVLGVQWVTSNTTVSGFSLTEAQKTIYIDASFGNVTDTEGLTLIPPTALEFAETFREDISTLFGANWTLQRVDQLPSSGILLGHFRGNERDLTYENGIVTEEGYEVEVANGSVYVGGTGARGMFWGTRSLLQGLLVANGSLPSGRVVDAPAYATRGYMLDAGRKWYTADFLKELCTYASFFKMSEFHYHSSDNYPLNRGHNETWSKVFSHFSLYPEENTELQGLIQRRNETLSRADFEDLQKHCAQKGVTVIPEIEAPGHALAITKWKPELALAKKDLLNLSHPDAIPTVKAIWSEFLPWFQTKEVHIGADEYDSELADDYINFVNEMADFVNSTSGKRIRIWGTYEPSSNLEISKDIIVQHWQYGQSDPIELQDSGYDLINSEDWWAYMSLKNDHMPILPAPYPQFYNVTRTVNFANVPGWQWDPSLFNPVNTTEQLQPDASGNKGAILAAWNDNGADATTQLEAYYAMREGIPAMAARAWAGKRGVGIQADKLAEGIAFLAPKAPGQNLDRRLPNSQTPSQTSPLLSWTRQSSDTTTINLDKASYGPPYTLTLNVSSSFTLSGPDTSLSLVATNSSINNNNTHPYTLIFSTADNFPYPLRSVSPLDGHDPGHPGRIWTNQSSSSHEPVTITLPTTLRIETDIRNGSRVWVNDEFAGRFEVFVFGGRNTLFSWSQMALVAPLGKIKGGVDGLVLEEGIGAGQSGSGNGSYVGVPGQSAAGRVGVSGMGMLVFGVVMLNLLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFX1</accession>
    <name>A0A9W8YFX1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001548</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFX1"/>
    <dbReference type="OrthoDB" id="5046242at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF414">
        <property type="entry name" value="CONTAINING AMINE OXIDASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12150)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="1">
        <location>
            <begin position="65"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="401" mass="44767" checksum="32FA5938641167C6" modified="2023-11-08" version="1" fragment="single">MPGKEASENTEVVWTIIEEAMKLSNEKSSSIPVGKSLYSFFEEQVEEKFPTKTEDTSDQEAKRKQRTILQMAEMWGAFVGSPIQKQSLKFFWLEECIDGENLFVAETYHKVLAKIAEPALETDVKFGYKVKKIKSDDEANDPSVLIDIEGKDSEVFDEVVMTAPLGWLKRNLDAFVPELPIRLREGIEAIGYGHLDKVYINFATAFWNKSSSTDALPSASQDHSLPNVTATTAPLHQSLSSDSSDSPVDPSHYPGFTHWARPDYAPDTNPHQWNQEAVNLAALPGETAHPTLLFYIYGPTSIHIAKLLASHPSSEHDALLTTFFQPYFSRLPNYSPTDPSHQPKSLLATAWANDELAGYGSYSNFQVGLERGDEDIEVMRRGLPERGLWLAGEHTSPFVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFZ0</accession>
    <name>A0A9W8YFZ0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_001882</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFZ0"/>
    <dbReference type="OrthoDB" id="295274at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14687">
        <property type="entry name" value="bZIP_ATF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051027">
        <property type="entry name" value="bZIP_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002112">
        <property type="entry name" value="Leuzip_Jun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19304">
        <property type="entry name" value="CYCLIC-AMP RESPONSE ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00043">
        <property type="entry name" value="LEUZIPPRJUN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="179"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="100"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="269"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="204"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="127"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4374805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="329" mass="36681" checksum="E2C07064D2BEC950" modified="2023-11-08" version="1">MMEIQTPHSFDWAHDAYLALNDPDTKHQQHDNWAPFTTAGPLMTSGFTIPPSLLTHATIERFGQVTPPEELSPAKPRLDTAREDSIPVDELLNETSWSHNGTQANKHAQSSQQDSQESSPKRRRTSRQTSNSKSANSAGPQQGAEDGNPQPPKRKRGRPKSQPQMVEAFTADGFPFQVSSARQSHLEKNRVAAHKCRQRKKEYINGLEGRAREFSSKNKALKENVALLREEVLSLKNEVLRHAGCGFWAVDEYLARCAGDLLGMEAPRHTLPSHQSKNKSPELALTLSNEEFEHFGSPGSSPTGSSPDDEFGGLELLQDFEDEEMDYQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGG6</accession>
    <name>A0A9W8YGG6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Putative gamma-glutamylcyclotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_000120</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the gamma-glutamylcyclotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGG6"/>
    <dbReference type="OrthoDB" id="1044435at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045038">
        <property type="entry name" value="AIG2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009288">
        <property type="entry name" value="AIG2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31544">
        <property type="entry name" value="AIG2-LIKE PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31544:SF2">
        <property type="entry name" value="AIG2-LIKE PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06094">
        <property type="entry name" value="GGACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Gamma-glutamylcyclotransferase AIG2-like" evidence="5">
        <location>
            <begin position="36"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="136"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="223"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4377296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="260" mass="28601" checksum="0118374B544C9948" modified="2023-11-08" version="1">MDLTTLYAHQHRRKPPSQSLSPPKPSSTMSSSHTAFFYGTLMAPPVLHRVIWGASSPPTPAHASLLRIRPAILHAHQRHKVKHADYPAVLPSESPSSTVRGTLVEGLTDGDIWRLDIFEGSEYERKKVNVRVLRGRGHNGDAGGEQQQATTDELVHGQGEEEKEVEVVEAETYIWTAGAHRLEAAEWDFAEFTREKMKRWVGAEAAETDEGFQEVDDAVAALQQQEQNQNPTGGRGANGVISQQLKQGGKAEDTILESAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGH1</accession>
    <name>A0A9W8YGH1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enhancer of mRNA-decapping protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">EDC3_1</name>
        <name evidence="8" type="ORF">N0V83_001570</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EDC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGH1"/>
    <dbReference type="OrthoDB" id="10030313at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10260:FF:000007">
        <property type="entry name" value="YjeF N-terminal domain-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="entry name" value="YjeF N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025762">
        <property type="entry name" value="DFDF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019050">
        <property type="entry name" value="FDF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13612">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13612:SF0">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09532">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01199">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="YjeF N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51512">
        <property type="entry name" value="DFDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DFDF" evidence="7">
        <location>
            <begin position="354"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="YjeF N-terminal" evidence="6">
        <location>
            <begin position="535"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="111"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="225"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="425"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="488"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="276"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="304"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="443"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4376287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="804" mass="85824" checksum="42CA7005712E2225" modified="2023-11-08" version="1">MASGFVGLSVAVTLQKPPNTVVYGVVAAVNPQTATLTLQNVFFPATGHRLSSYHVEGHAIADIKVNATAPAASHPVPPRPDPPPYSQYPPSHVQRAPADRVQQMPQLISQPLRQPASQPARQASLPHANQPVPQSAPQPPQASAFVDPAILSMGKRASLAPSSNQHVGAPPQEAPATPIKSISAATIASLPKNTSPFVGHTKAVRKPSAATLEGPFSSLDIADAEEADSETKTVQASVRRASINKTRTGKPMDDPSPQKAEDGSKKTRRGGKSRKKELAAQERKTGGDLNSNSDAVRKGKGNGWRNTPMLQDAEQSQARTPGVIGGRIGLEAANASARKSRRQKALEATNGWATEDATDIQEMPEFDFQGNLSKFDKRTVFNQIRNEDTTADEDRLVSFNRLARPGTHGGKNLHPTENVLDRKLKSTTNTSSEDDSDFGSGRNSKRAMSRASVKRAPTRQGSGVQAEHDVMGSGLLSRASRAFINRPYASSHATGSPKPGRITTPPDSPLESSTRGCLRLVSNNHRCHAVTSGGMLAVEETAEVDFGLTEELIAENSGRGIAEVALTAINPGGRRLARDNPNAVPVIVVLAGNHRGGARAVAAARHLQQRGPKVMVALLGFERNADWDKDVRRQVDLFRKLGGSVRAWKDTEEALKRLQAPPELIIDALLGRHKEFEALGDEDRRTVLGIVGWANKSRAACLAVETPSGVGGSTGEVAILEGEPLEVRAKYIVCLGAPRTGLLKALQNGAGRGDPAWLIWIVDIGVNKPWRNAGISGGKGIKFGEHWVVQARYDEAEEEDAAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGR4</accession>
    <name>A0A9W8YGR4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ran-specific GTPase-activating protein 30</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">YRB30</name>
        <name evidence="2" type="ORF">N0V83_001690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGR4"/>
    <dbReference type="OrthoDB" id="512915at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030695">
        <property type="term" value="F:GTPase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008812">
        <property type="entry name" value="Ran_GTP-bd-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31010:SF2">
        <property type="entry name" value="RAN-SPECIFIC GTPASE-ACTIVATING PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31010">
        <property type="entry name" value="RAN-SPECIFIC GTPASE-ACTIVATING PROTEIN 30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05508">
        <property type="entry name" value="Ran-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="608"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="635"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="665"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="687"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="710"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="755" mass="83223" checksum="9174867557EBD3C3" modified="2023-11-08" version="1">MDILLAKVTQQAMTYAIRSGIAITSTYALKQCSRLMKTVDGYEKQELATLQLRLDSKIKIISPAIDMIELIAARGNTSLESAVTLTKSLRWEIQTLGSRVEQAVAEEQLSRRGSSKAKSRAQNDVELKIIISEMRKLLDRIEDAVPLINLAITTSGVSLNTTLPATVSPSRLLQASTFLTAGDMQYCSSKPRAQQIGPTFTLSMYMLFSGYANRPHGDSFRETTWKEVMHKARVTLLRVPLDHVYDYPTPFGPDTLRPHEGNHRHIPSDSALQEFAYQLLVVEDLDDGRMHEFEDGEPQPGPYDGIEQAGVREIIPIHEISKIFYADTGKILNIGTDGETNNPILLLKRDVNAAPPRRMMERDATEQGYDSDDSHTVGDDGADLEQSELEDQLRRESTPNLQEQLVDEPEQPTFPWRLPPTLDLEWLAFEVFSEDPSTDSEVDETEGSEFDVASPTSAEPVAQRNRSESPAFISRLSNLNLRPSTPSETSPPPSNTQLIPSPQKANVPVPQYTSQAPTTLPPIKSSLSLLEMLIRLTALQQFQQSSHLAIPDEFLNFFLSDSSTVGAGSDADLRRRVRREARMRVGFDPYDESPIKRRGEEYLEHELHDGQQHVYGATSPVDGGTPYDYEGYPTSRASSIYSSRSRSGTPARSPSSPSFPNSTPAGPSSRSKSSANILTEGRPSLAHVSSPSPLLRRATTQPNQHNIMRQPSFRAPPTAPPTSSSPRQAPAAPGVQAFRGSVAPDTPPSSVGKQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGV9</accession>
    <name>A0A9W8YGV9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_000526</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-binding monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGV9"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050775">
        <property type="entry name" value="FAD-binding_Monooxygenases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43098">
        <property type="entry name" value="L-ORNITHINE N(5)-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43098:SF3">
        <property type="entry name" value="L-ORNITHINE N(5)-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="63225" checksum="BAD6B41303437D20" modified="2023-11-08" version="1">MVHFRTDIGATNESATQNSENYAKELETDVLIVGAGFGGIYLMHKLRQQGFKCKIFEAGADIGGIWHWNCYPGARVDSQVPIYEYSLPEIWKDWTWSCRYPGTEELQKYFEHVEKKLDIKKDCAFNTRVIGAQYDKPSSQWLVRTEDGRTGKAKYLLLALGFAAKRHFPDWPGMDKFKGEIHHSSFWPASGVPVKGKRVAVIGTGSTGIQIAQETAKEAATVTNFVRTPNLCLPMQQKLLTKEEQDKRKQGEYQEIFTNRLNTFAGFQYDFVEKDTFDDSPEDREAFYKKLFEAGGFEFWLANYRDVLKNSEANRAAYDFWAKVTRKRISDPEKREILAPLEPAHAFGTKRPSLEQNYYEMLDRPENKVVDVKKTPIKEFTEKGIITSDGKLHEFDVIALATGFDSVTGGIKNMGLKDVDGVELAEKWKKGTYSYLGVSLSGFPNCFFLYGAQGPTAFSNGPTCVEVQGDWIVDAIVKLRNEGINYCDATHEAEKEWREKVIELSDETLFPQTKSWYMGDNIPGKVREQLNFAGGFPLYSKLLRSALDDGFRGFITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH66</accession>
    <name>A0A9W8YH66_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Radiation sensitive protein rad9</fullName>
            <ecNumber evidence="6">4.6.1.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RAD9</name>
        <name evidence="6" type="ORF">N0V83_000769</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.16" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="129353at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17745">
        <property type="entry name" value="BRCT_p53bp1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047249">
        <property type="entry name" value="BRCT_p53bp1-like_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041297">
        <property type="entry name" value="Crb2_Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047252">
        <property type="entry name" value="TP53BP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15321:SF3">
        <property type="entry name" value="TP53-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15321">
        <property type="entry name" value="TUMOR SUPPRESSOR P53-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18115">
        <property type="entry name" value="Tudor_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="5">
        <location>
            <begin position="1259"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="161"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="299"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="379"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="731"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1207"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1507"/>
            <end position="1529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="177"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="229"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="347"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="433"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="474"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="496"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="554"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="731"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="786"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="877"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="981"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1207"/>
            <end position="1219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4377939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1563" mass="169971" checksum="EAA2F03EE6BC6431" modified="2023-11-08" version="1">MAESQALTVLADTLYDDPSQLSQALRQRAGSDKPLHFTTSTESHHSGNTASAFTVSPQLSRALVPQQPPVVLARVDHAPSSRRVATANYVKPALPQHHSAPTSTGIVAEVVGAYKMHHSFSVMDAPGDTQPDSQMHKQWTSGVFPTVEANNRPVSLFTEHVDEEEEENDLPTDEVRASQSSQQRNNPTVTSPTVFDIDDRAPAEQHSLALTSPLKFETPALAGRKRDNSGQMLSSAARSNETPGTVVSASAFFPVALGNGAIAGGPLMSLTQAWQNTQAPTSPAVGGASEDVVFTRPSPNFTNARHSSPIPAYSSPIKAIRPETPKSDPVIRSSSEPRAEYVTMKQSQEKRKLSTGDERVTAVEQDSWQELSVKMKRRKAKEQFHQRAARSLSNISAPTPLVPRSGRKRAVNAKSSSPVRLKTPRRIAHADPEGEEDDDSMDELLRPTPRKRHDAEDSPDELSQDVPFKARTTVKYNSNDNRVQVPKTSSHPHRTPSGQALRQSSQHATPSSQLQRESRSRPPGSQPRLRDTLRPSSSKDSVAIMDSQPDATADYQSIPQPKSLRFPSSPSTNEYSINQTTMATKTGYTSQVISSSMPPLPPRSSPTPSDDVLNEEVMADEAERVPSSPPILTDKDAATHEEEIEYDEHAYDEYGADVENHGENEPSVVDDDILMDEDEDLPVANQLPDQKAVAGKEVDVADDDELDLVQPEKVDLGTDEAVPSASLYVLHEKQTYRDEDSSKAVEYPHPPRMQRQNTVPETDALDETQPSCFPDNVQDDLASRTEMANHTNSTEPFQTAQEEQHGSQSNRPAPLSSREEGSDLIQLGNRIRSINDVHNLPATQQSIPEEEIEMPRLSGLDEEDPFLPRSSPAPPSAKRRKVTYTAKRKAFRSPVKPLTDSEPLSDPPPSSPLDGVQEVLADTPPKAPAREQEKQGGLAVVSTGGEAPYARPATRKPAGLPRSLKAQAQRKGALKPITRELLQSLSSPNKSPSKLRGSLTSRASTPPSPSRRRADAGDAQTNIDMIDVDNETDELAGPTPQTANVDSFVEIASNRDTTPSGNVLVPNRVFASWPGSHYYPATCIGRSTSRQLQVRFDDGNDTSLDAVHVRALSLRTGDHVKVDEAGMKKNTYVVVGFKDKIDDLGGEEYPTTDQLGFATIVLEEKRRDSLPAAKAILATEQVSVPMASIYLTTGLWTRLRDRSFNFSPPTSPNKATSRIGTPVIDPLATPSFTRRGTTAPSLLKDVTGRAASVASTARSGTSVFSNMAFVLTSTAADVDKDAIAKLIKSNGGQVLEHGFHELFDYESAEATSSQSRRRSISADEGSVGLALKHTRKDLGFVALISDSHSRSTKYIQALALNVPCLHLRWIQDSLAAARALSFAKYLLPAGVSKFLDPNGVLRSRTMSIYDPAGDDVSFAQTIQERDLLLHNQAVLLVTGKSKKEMERRQPFIFLTHALGSANVGRCADLAAAAQMLTEGQWDWVYVDNGERGVAEAAAELFGRTNGAKSGGAVKAKKGKKRKADESDEREELVARGELAGRKVRITCAEFVIQSLILGALVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHG1</accession>
    <name>A0A9W8YHG1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ATP-binding cassette transporter snq2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SNQ2_1</name>
        <name evidence="12" type="ORF">N0V83_000410</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001010">
        <property type="entry name" value="ABC multidrug transporter (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="537"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="572"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="614"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="646"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="679"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="706"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="785"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1205"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1234"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1273"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1313"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1341"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1465"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="169"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="864"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="81"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4377583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1491" mass="166902" checksum="CD9CA87E2AF4DA81" modified="2023-11-08" version="1">MDNGNREQSVARTYEPIHADSHNIQRDNDSVRTASIDNPTARNSNDFNEKTGFTNIKGGVDIQRAEAEFAELSKELSRTSHISRRLSRVQSRQSRKDQVATDVEKGAIAGSEASSDEPFDLEKTLRGSRDEEEAAGIKSKRIGVVWEDLTVSGIGGVKNYVKTFPDAFVSFFNVFETASNLLGLGKKGKEFDILKNFKGVAKPGEMVLVLGRPGSGCTTFLKVISNQRYGYTNVNGKVLYGPFESSFFEKRYRGEAVYCEEDENHHPTLTVGQTLDFALETKVPGKRPAGLSRQDFKQKVIDLMLKMFNIEHTRNTIVGNPFVRGISGGERKRVSIAETMITGASLMSWDNSTRGLDASTAVDYARSLRVLTNIYKTTTFVSLYQASENIYKCFDKVLVIDNGRQVYFGPAETARAYFEGLGFMEKPRQTTPDYLTGCTDPFEREFKPGMSEKEVPSTPDALAEAFNNSPTAARLATEMTEYRTRMDEEKHVYNDFQIAVKESKRHAPQKSVYAIPFYLQVWALAKRQFLLKWQDKFSLVVSWFTSMSIAIVIGTVWLDLPKTSAGAFTRGGVLFIALLFNAFQAFSELASTMLGRPIVNKHRAFTFHRPSALWIAQIGVDLLFASVQILVFSIIVYFMTNLVRDAGAFFTFALVIIIGYLAMTLFFRTVGCLCPDFDVAIRLAATIITLFVLTSGYLIQWQSEQVWLRWIFYINALGLGFSALMMNEFKRLDLTCAGTSLIPYGSGYGDINAQVCTLPGSHSGSTSVSGTDYIKTSFSWDPKDLWLHFGIMIVLIVGFLLANAFLGEFVKWGAGGRTVTFFVKENTELKTLNTKLQEKRDRRNRKEVGANDNSELNITSKAVLTWEDLCYDVPVPSGQLRLLNNIYGYVKPGQLTALMGASGAGKTTLLDVLANRKNIGVITGDKLVDGKVPGIAFQRGTAYAEQLDVHEPATTVREALRFSADLRQPYETPQEEKYAYVEEVIALLEMEDIADAIIGDPESGLAVEQRKRVTIGVELAAKPELLLFLDEPTSGLDSQSAFNIVRFLRKLAAAGQAILCTIHQPNSALFENFDRLLLLQRGGQCVYFGDIGKDAHVLLDYFHRHGAECPADANPAEWMLDAVGAGSAPRIGDRNWADVWADSEEFAEVKRHIVRVKEERVAEVGSTEPTEQKEFATPLSYQIKQVVRRQNLSFWRTPNYGFTRLFNHVIIALLTGLMYLQLDDSRTSLQYRVFIIFQVTVLPALILAQVEPKYAIARMISFREQMSKAYKTFPFALSMVIAEMPYSILCAVFFFIPLYYIPGLNSDSSRAGYQFFVVLITEIFSVTLGQAIAALTPSPFIASYVNPFIMIVFALFCGVTIPKPQIPKFWRVWLYELNPFTRLIGGMIVTELHGQSVTCKPNEFNQLSAPQGQDCGSYMSDFFSSGAPGYLLNNATNMCDYCAYKVGDEFYQPLGYEFSNRWRDLGIFAAFIASNLIILFVAARFLNFNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YI32</accession>
    <name>A0A9W8YI32_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oxysterol-binding protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">kes1</name>
        <name evidence="3" type="ORF">N0V83_000938</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the OSBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YI32"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008142">
        <property type="term" value="F:oxysterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120009">
        <property type="term" value="P:intermembrane lipid transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2720:FF:000003">
        <property type="entry name" value="Oxysterol binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000010">
        <property type="entry name" value="Oxysterol-binding protein homolog 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF184">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN HOMOLOG 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4378106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="399" mass="44827" checksum="B0451CD9C907AFF7" modified="2023-11-08" version="1">MAEKEGSAVPAQAKGSWTSFLKSIASFNGDLSTMTAPAFILSTKSLTEFSSYWTEHPSVFVAPAAEKDPAKRAMLVLKWFLSTLKQQYASRSEKLGSEKKPLNPFLGELFLGKWEDQAGTTELVSEQVSHHPPVTAYSIWNSKHGVRLEGYNAQKASFKTTINVKQVGHALLHLDAFDETYLITLPSLHIEGLIMGAPYVELNSNTYITSSSGYTSRIDYSGKGWVSGKKNTFSAILYPTGKEKDAIYKVDGQWNTSFQVKDAKTKAVVDTFDHKAFKTTPLTIAPVEEQDNFETRRAWKKVAEAINKGDMDLTSAEKSIIENRQREMRKDEKETGREWNRAFFTRVQKFPLFEKLASQIGEKVNDDQTGGVWIFDKEKANAAKSPFHPDVKPPIYERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJ88</accession>
    <name>A0A9W8YJ88_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_000121</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="442460at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070139">
        <property type="term" value="F:SUMO-specific endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051947">
        <property type="entry name" value="Sentrin-specific_protease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46896:SF3">
        <property type="entry name" value="FI06413P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46896">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="7">
        <location>
            <begin position="571"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="62"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="230"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="507"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="695"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="926"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1234"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1286"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="230"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="276"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="514"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="926"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="979"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="992"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1070"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1182"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1286"/>
            <end position="1296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1312" mass="147636" checksum="6FD185998D0B3EE6" modified="2023-11-08" version="1">MNKPGSNNSFRPVNTINEINPAARTRMTTYSAKHSRRSVAPIGQTRHAGQLAYLDRERETAPTATAHNVFSRTEQARTAGGASSAKRRRSEVIIDVSDDEDVEDNNNELRGVEPPSVSYARAGTSYDMPLSARSSQSKSLSHLSDGSGSAITTVKHPSQPKSAFLATEDFFEPHRKKQRTPSSNRRQGTQRIPSLSGNSFSGSFEVHEPIVTESVHENVIGRHESARRSILDDFKQGESARSSDERQQQSRRHDTITSRHFTKARINESTSEKLVDNQQTKRNATELRNQYRPAPSKHAVTEGSEDELSMPNPKEMATGRNARRGGSISKARQAANSGVKRRVPWKAANEGWPLVFARSRDYVCHGSSMDDGRAQIYLRQDSDCWRVFTFDQTEGEFSPRICIRPQDVNTVNVDDDGTRVRLEDRTFKYFARSMQHPWHFEEDFFRDKDTMAKLFSKPLKSNDKVGTSELVHDAITEADDHTQADRQALNTPLLDQLRNISRGENEHQKLEAVSRPVRISTRPTRSTRSSLPSYETEATNNAPKVTKYSVDVGLGLPWNKPVEYGQGRQRAVVDFNDLPRLDEEEYLNDSLIDFYMIYLFNKFNVPSNKVYFFNTYFYTQLTKNTGRQSMNYKAVERWTSKIDVFSYDYIVVPINEATHWYLAIICNVANIDQTSSIEEVDKDLEEALERSRQDLVKADESADKPQPSEPLLGSNNAKPDHTEQIDDDPNLFEEESGLNIVDREDPGVEIRVHDNQVSPASHSPAAETVHAARGASDTDIIPKGILTNLVASPERRKGKRKFTQQKKDPNQPIILILDSLGSVRSGTVRALKDWLAAEGAAKRGIEATIKEKGYYPKASQIPMQSNWTDCGVYLLGYVEKFFQNPDEFKNKLLTAEMSADDDWPELKPKDMRTNMREIIVQIAKEQEVARRKEKKAKKESSSAKTSPAPIKEPAQPEMQVSQLAQVPSMGPDDDPKEPANLNATNAETNESDAQHPIEDTRPRLGSPFNPEPQHIKTATRSPTPVAAKEVSQGPISPVKAIGTLPHLRHSPRRISPEVRIPIKTPQSVASERRGKTSSDRTERQSEHVRSPVNAFQSSSPTKRPRQDEDDSQLRLPVAKTHIRSPRQQKSNPIRRSSPLMPRRREGSADRPIQINDSQDEIPVAITAFKYQQTAGASSPRKTPSRTPRRPNVLRHTSSIEEIPGPAAHAIARHHSPRGQQLIGHLLEAELDHADHERHRTRRSTARSPTTPKKNGSLYQKRGTEAMTIDLESQDADTMDLETYEARPDAMVRETPEALRSSPRLHKDGSLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJC4</accession>
    <name>A0A9W8YJC4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PEBP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000407</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJC4"/>
    <dbReference type="OrthoDB" id="2506647at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030414">
        <property type="term" value="F:peptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030162">
        <property type="term" value="P:regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046578">
        <property type="term" value="P:regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF141">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040993625" description="PEBP-like protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="25265" checksum="B1A6E5401FAE85F4" modified="2023-11-08" version="1">MYLSSSIILAAVIGFAQAQTPSGFTPQVNTKLDVLFNSTSVATPGQKLSKASTATEPQIAIPSSMVSRNETFMFVMLDLDVPPTQGNQTRRVLLHALNTGFKATQQKINGTAVLLTSPEKGPAMYLPPGPPATDTVAHRYVQLLFKQPADLQVAQSEFADTQDRINFNIQSFASENKLSAPIAANFFRVDGRDSASATGTATGTGGIVQDTLVPFEGGAGQLAYSHGLAGLVGGLAFFAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CGK3</accession>
    <name>A0A9W9CGK3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7719 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_010461</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CGK3"/>
    <dbReference type="OrthoDB" id="5597489at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056136">
        <property type="entry name" value="DUF7719"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37846:SF1">
        <property type="entry name" value="DEACETYLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37846">
        <property type="entry name" value="YALI0B21296P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24841">
        <property type="entry name" value="DUF7719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="129"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="196"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7719" evidence="3">
        <location>
            <begin position="159"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4362368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="228" mass="25200" checksum="6D32E30F0F7497BC" modified="2023-11-08" version="1">MAAGNRKERRAKESQTNAKSTGSAFQPSNELDQDAVEMILKHPDYSGPKGKTLFQLAEERQKELDKGKSPAGKVDEDKASARDAPLDGEPAIGPVGDAILYATSMAALHLTLDVIVYSQYREDILWGEIFRRAATATPIFILLVYLTHVDLSYRFPIFRDIAFLAGSIVAGCYLVYSGNKHGYFHVMKAAPPVGTLWIWGVVEMSLPFAALSAIAVFGFTWWNGFEFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHK7</accession>
    <name>A0A9W9CHK7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Gylcosyl hydrolase 115 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_009477</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHK7"/>
    <dbReference type="OrthoDB" id="4849794at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.379.10">
        <property type="entry name" value="Chitobiase/beta-hexosaminidase domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.520">
        <property type="entry name" value="Glycosyl hydrolase family 115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031924">
        <property type="entry name" value="GH115"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041437">
        <property type="entry name" value="GH115_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042301">
        <property type="entry name" value="GH115_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029018">
        <property type="entry name" value="Hex-like_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37842">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37842:SF2">
        <property type="entry name" value="GYLCOSYL HYDROLASE 115 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17829">
        <property type="entry name" value="GH115_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15979">
        <property type="entry name" value="Glyco_hydro_115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55545">
        <property type="entry name" value="beta-N-acetylhexosaminidase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040763403" description="Gylcosyl hydrolase 115 C-terminal domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="domain" description="Gylcosyl hydrolase 115 C-terminal" evidence="4">
        <location>
            <begin position="839"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1013"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1023"/>
            <end position="1032"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4364022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1032" mass="113524" checksum="ADB0AF656026915C" modified="2023-11-08" version="1">MELFSRTVLGFLSLSVAFAGPATAVESFRKPTVGTQRGNGSTLLASRGSAVQILADAADWPGVLRAANDLAVDFGRVTGTNGTLTALGTGTANASAIFNVTGINADWSVGSSGNSTSTSATAGTIIIGTIGNSSLIDNLIASGQLDVSAIKGQWEAYVSAVVENAGNGTGEALVIAGSDRRGTIYGLYDISEQIGVSPWYWFADVPPKSHESIYALKTTKVQKTPTVKYRGFFINDEAPNLTGWADKIYPLSPYGAPFGADFYKHVFELLLRSKANYLWPAMWNGMFNVDDPRNQPLADEYGIVMGTSHTEPMVRATQEWTKIAKGTDVSWSWATNNATLYKFFYEGALRAAPYENVVTVGMRGYHDTSMSADVQTDLLEAVVNAQQEILNKIHGNSSEVPQMWCLYKEVQDYFEAGMQVPDWVTLLWTEDNWQNIRRLPVGDEKKRSGGAGVYYHFDYVGDSRNYKWQDTIQLQKTYEQMRLAKDRDADRIWIVNVGDIKPAEMAISHWFDIAYDIDAYDETSVPQWIAGWAARNFDDEHAQTISDIMDEYGALANMRKFEWVEPSSYSVLNYEEADHILARWQALAQKAQEVNSALPAAQQPAFYELVLHRVLAGGNFVDIQIAGARNIIYAGMGRNSANKWMQRVINGMNQDHNLTKIYHTQLNGKWDHMMDQTHLGYQGYWQQPMRQSTPDLRWVQTLERSLAGDMGVAVEGSNATIPGDDQWHSNGGGSLNLLDITPFTPKRWLEIAHRGTGPFSWNISADPFITLSQASGTMKPDDDDLRVYVSVDWTKVPDGFGAKSTLNISSSTDYGTQGGAPQVIVQVNKTSIPSSFSAGFVESAGQLAIEAEHYTRTSSAGNLSYMVLPKYGRTLSAVKLNNALAEGLTSSTGPSLEYDFMTFTPTTSAKGLNVTLILSPTLNINPKKPLAYIAQVDDEPEQRRQYVIDQPQPNFPVGWGKAVADSVWYNTTNHGALAPGKHTLKLWLVEANVILQKVVIDLGGVVYSHNGPPESYRVGGGSSNSTVTRRHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHL7</accession>
    <name>A0A9W9CHL7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010051</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHL7"/>
    <dbReference type="OrthoDB" id="4524534at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00413">
        <property type="entry name" value="Glyco_hydrolase_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040737702" description="GH16 domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="2">
        <location>
            <begin position="14"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="223" mass="23984" checksum="FC03527917B11CAA" modified="2023-11-08" version="1">MHFFSLSVAALLLSTASATPHHKRATTLIPKTVFDSTASFEQYFTYNYPWGGTTHNGGARMDKAHVSISPPGTLTLTAQPAKNQPPATHAGKQIPINYLSGAVGAKQHFTVPTGGGLLFSASVLATTTKGTWPAFWLTAVSGWPPEVDMAEWKGSGKISFNTFNTSSSAQTKDVAYPDPGAFHTVKCQLRDNGGGVVQVKYWLDGTEVTTHYGRDYVGKELYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHM8</accession>
    <name>A0A9W9CHM8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010674</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHM8"/>
    <dbReference type="OrthoDB" id="2398441at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033768">
        <property type="term" value="C:SUMO-targeted ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038886">
        <property type="entry name" value="E3_SLX5/Rfp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28042">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE COMPLEX SLX5-SLX8 SUBUNIT SLX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28042:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE COMPLEX SLX5-SLX8 SUBUNIT SLX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="454" mass="50770" checksum="12996CF145EFB29D" modified="2023-11-08" version="1">MEGHERSASPAWTFNTRQPSLSFHSSLPLYRGRDYEPPPHYYNYDERRTPAFEPSTRFSSIYEPINLGHEPPTDRPASTSALGPNTSGLHFPRIENMASSAPRRFAGDGLDYRRPISSARELDAVDLTREEEAIDLSRDEDVIDLTADDSGYGASQDGNNTGSHEIDGGGENANRSRRTEIGAPRLPRGMDIIIDLDNGEEQWRMASPPVAEPEPASPEIEYISSRRIDQPRRPPMFGRSNSDGDEVEFVRENALPQEEVRRRRNQEIDNVMDLLGTMNGRFTHLRAQVDRFNAQVNRTADRFRRGPIPPPRGAPRAHAHVRIGAFMAPALDFEMVGFDIGMPGVRAQEPPPPTYEAPERAPEGFTRSPEEADVLVCPNCGDELCVGEDEVKRQVWIAKGCGHVYCGECTTNRSVKRSAKGKERPTRTKPFKDCQVEGCGKNVANKKSMIQIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHT8</accession>
    <name>A0A9W9CHT8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ATPase</fullName>
            <ecNumber evidence="4">3.6.4.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">AFG1</name>
        <name evidence="4" type="ORF">N0V83_010145</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AFG1 ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.7" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHT8"/>
    <dbReference type="OrthoDB" id="548867at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005654">
        <property type="entry name" value="ATPase_AFG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040713">
        <property type="entry name" value="ZapE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169:SF6">
        <property type="entry name" value="AFG1-LIKE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169">
        <property type="entry name" value="ATPASE N2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03969">
        <property type="entry name" value="AFG1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4363027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="465" mass="52850" checksum="65F01A1548217F0F" modified="2023-11-08" version="1">MAEYDARVESGRLRDDEHQRGIIKNLEDLHNMLLNYSQPTVRQPTIESLQPPKKSFFSFFSSSKPASTALPPIPDSLPKGIYMYGDVGSGKTMMMDLFFDTLPPNIKRKTRIHFHAFMQSVHKDLHKMKMQHGNDIDLVPFVAADIAQRSSVLCFDEFQCTDVADAMILRRLMEALMAHGTVIVTTSNRHPDDLYKNGIQRESFIPCINLLKSRLTVLNLDSSTDYRKIPRPPSGVYHHPLDASAHTHAERWFRFLGDFDHDPPHKAVHEVWGRNIEVPKASGKCAWFSFNEIIGKATGAADYLELMRNYEAFIITDVPGMNFKSRDLARRFITFIDALYESRAKLVMTTAVPLTSLFLDASELNEAVHASQEASGADPAENHESVSDVMRHLMDDLGMNMSMLKNSSIFSGDEERFAFARALSRLAEMGSQEWVERGLGMEKKGGRAEMEGWQRVRSRWREDSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI22</accession>
    <name>A0A9W9CI22_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009697</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI22"/>
    <dbReference type="OrthoDB" id="1862401at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051283">
        <property type="entry name" value="Sec_Metabolite_Acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896">
        <property type="entry name" value="FAMILY REGULATORY PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G14730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896:SF13">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="225"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="491" mass="55199" checksum="D03610AADC71A07C" modified="2023-11-08" version="1">MPRVKEFQLCPFGWENDPAEERYKLGTLDYLSTTTWTNTAIFFKLEDAEKAKAEAILKEGLERTLSQIRHYAGTIERDEDGHHSIVKKKNSTVRFVVQHLDSPEDKYPSFDKIAKAHFLSTVLGDINVLSNAPMTCGNKPEAHPDNHPAISSYKANFIAGGLILNVHTHHYCNGLSGSTAFFKQLAGNCYALANSTKFPTWDLKWLDRSLYGVPGFDRPSNSDKPLLDAPRRPERNVQHKPSQSVMFHLPKSKATKLKKAAMPSDGSRISTYNAVCALMWRVLSRVSEPLYKPGLDYKPLWAEGVSISKLFTNPPIPVQMQGNLQIDIKSTTSSLPQLTLAEIISEAPLSRLATFTRQMTDSVTADMLAETLEKFTQVRNKQDLSIYLDSFPPMSLLVSDWRYIDMCTFDYGFAFPTAFRHLFGGVPLSQAIVYPPHKGPAGDDEGMEVQFTLEIELMPELLKDPEWNKYFDFRGVDATEEGPLSKHASKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI87</accession>
    <name>A0A9W9CI87_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ER lumen protein retaining receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_009384</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI87"/>
    <dbReference type="OrthoDB" id="7694678at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046923">
        <property type="term" value="F:ER retention sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000133">
        <property type="entry name" value="ER_ret_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10585">
        <property type="entry name" value="ER LUMEN PROTEIN RETAINING RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00810">
        <property type="entry name" value="ER_lumen_recept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00660">
        <property type="entry name" value="ERLUMENR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="279"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="310"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="350"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4363931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="40489" checksum="2B3183AF60990B04" modified="2023-11-08" version="1">MKFNLFHILGDVSHTSSKLILIWAIHSNSSAEGVSLITQALYALVFGTRYLDIFTSSATKDVWHTWNFSLKIFYTLSSLYIIFLMTSVYARTREREKAWKFGMYCLLGSVVIAPFWYLIFKQWVIGTSTFLRMLWVFSEVLESVCVIPQLLLLRQTTVPTVIDSFYLVTLGAYRFMYVLNWIVRGANEKGYADPTSWVWGSIQTALMIDFAWVYWTRQRVKLRRGGVVDSDDLGRGWIVGRFVGRKSVDFDFDEEAASGQGNNTVPPKNKWGRRGISVSADEGVQGAPKRKGAQSGNVSAESQPLADPAAFEDESDDEDAPPPAFAGSSAAAHESGVRGDEWDDDVDEDAREHEGAAKLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIE8</accession>
    <name>A0A9W9CIE8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Phosphatidylinositol-binding protein scs2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SCS2</name>
        <name evidence="9" type="ORF">N0V83_008915</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIE8"/>
    <dbReference type="OrthoDB" id="264603at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160219">
        <property type="term" value="C:cortical endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140506">
        <property type="term" value="F:endoplasmic reticulum-autophagosome adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160214">
        <property type="term" value="F:endoplasmic reticulum-plasma membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033149">
        <property type="term" value="F:FFAT motif binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090158">
        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051685">
        <property type="term" value="P:maintenance of ER location"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902647">
        <property type="term" value="P:negative regulation of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000813">
        <property type="entry name" value="Vesicle-associated protein 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016763">
        <property type="entry name" value="VAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809:SF6">
        <property type="entry name" value="AT11025P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019693">
        <property type="entry name" value="VAMP-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="MSP" evidence="8">
        <location>
            <begin position="2"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="131"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="234"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="136"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="234"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4365296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="292" mass="31484" checksum="A20FC8EE95970247" modified="2023-11-08" version="1">MSVELDPVELGFNRPFQQEVSQTLRLKNPHSDPVAFKVKTTAPKQYCVRPNSGRIEPGKDVEVQILLQAMKEDPPPDAKCRDKFLVQSVLVTADKEFTNVGSLWTHIEQTSKSSIQEKKIRVLFLPPHDPFGTATPAKTNGVSGDSMLSSPSPEAVTPQRGVSEAAGPVSLPRDRPADSKNLGEIKDETYNPATSGGVQSTVAAAAASVKNALPSQGDLQTQLDEAKAQIARLTQQAGESTGLRQRKTESTRDAKEQLSSAVDTRQAPAGGVPIHITALLCLISFLLAYFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIF9</accession>
    <name>A0A9W9CIF9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pectate lyase</fullName>
            <ecNumber evidence="10">4.2.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_009553</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Pectinolytic enzyme consist of four classes of enzymes: pectin lyase, polygalacturonase, pectin methylesterase and rhamnogalacturonase. Among pectinolytic enzymes, pectin lyase is the most important in depolymerization of pectin, since it cleaves internal glycosidic bonds of highly methylated pectins. Favors pectate, the anion, over pectin, the methyl ester.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>Eliminative cleavage of (1-&gt;4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.</text>
            <dbReference type="EC" id="4.2.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 10">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the polysaccharide lyase 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.2" evidence="10"/>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIF9"/>
    <dbReference type="OrthoDB" id="441042at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030570">
        <property type="term" value="F:pectate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004898">
        <property type="entry name" value="Pectate_lyase_PlyH/PlyE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33407">
        <property type="entry name" value="PECTATE LYASE F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33407:SF9">
        <property type="entry name" value="PECTATE LYASE F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03211">
        <property type="entry name" value="Pectate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041016572" description="Pectate lyase" evidence="10">
        <location>
            <begin position="19"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4364098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="256" mass="26264" checksum="1ECB2167E83BB61C" modified="2023-11-08" version="1">MKFTVAASIAALVATVAAGPAASTPNEFFSLNKRATLPVPASKGSVTYKKPQEVSGVFDGGMKTYGRGVSCTGQAEGGDSDAVFLIKNGGTLKNAIIGKDQIEGVHCEGACTLINVWWVDVCEDALSLKGDGNALIQGGGAQAASDKVVQHNGKGTVTIDGFTVMDFGKLYRACGNCKNSVSRSVVIKNVKAYNGKVLAGINPNFGGTATISGTCASSVKTICEEFKGTTPGNEPKSVSKGPSNYCKYTASAIKAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIL9</accession>
    <name>A0A9W9CIL9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIL9"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="152" mass="17464" checksum="FAD41AD64C5EB4B1" modified="2023-11-08" version="1">MEDGQDRGMEKERSQMHINLDEDTEALKLGIEKAEIRLAERKAEQMRSNMEAKWTAARLEGEKDHDEMVGGEKEKKMMEGYRIPKRARPVVVEEEEDREEEQPSSKKFKIAHSGPGHQPHISIRPHILTTTAGPRAHGHQHTDAGVYGRRDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIZ0</accession>
    <name>A0A9W9CIZ0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Mitochondrial fission 1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">FIS1</name>
        <name evidence="14" type="ORF">N0V83_009279</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Has a role in mitochondrial fission. Has a role in outer membrane fission but not matrix separation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The C-terminus is required for mitochondrial localization, while the N-terminus is necessary for mitochondrial fission.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the FIS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIZ0"/>
    <dbReference type="OrthoDB" id="421154at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12212">
        <property type="entry name" value="Fis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000179">
        <property type="entry name" value="Mitochondrial fission 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016543">
        <property type="entry name" value="Fis1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033745">
        <property type="entry name" value="Fis1_cytosol"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028061">
        <property type="entry name" value="Fis1_TPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028058">
        <property type="entry name" value="Fis1_TPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13247:SF0">
        <property type="entry name" value="MITOCHONDRIAL FISSION 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13247">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 11 TPR REPEAT PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14853">
        <property type="entry name" value="Fis1_TPR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14852">
        <property type="entry name" value="Fis1_TPR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008835">
        <property type="entry name" value="TPR_repeat_11_Fis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 12" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR008835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4364682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="153" mass="16971" checksum="E8BB840A855C7C34" modified="2023-11-08" version="1">MSPSLPYAADVESPLKPEELQVLRAQYEKEGEYVGLQTKFNYAWGLIKSSSRPDQQEGVRLLSEIFRNSRERRRECLYYLALGNYKLGNYAEARRYNELLLELEPANLQAGSLKGLIDEKVAKEGLVGAAIVGGVVVAAGVLGSLLLRGGKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJA3</accession>
    <name>A0A9W9CJA3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sister chromatid cohesion acetyltransferase Eco1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_008264</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetyltransferase family. ECO subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJA3"/>
    <dbReference type="OrthoDB" id="428854at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061733">
        <property type="term" value="F:protein-lysine-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028005">
        <property type="entry name" value="AcTrfase_ESCO_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028009">
        <property type="entry name" value="ESCO_Acetyltransf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45884">
        <property type="entry name" value="N-ACETYLTRANSFERASE ECO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45884:SF2">
        <property type="entry name" value="N-ACETYLTRANSFERASE ECO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13880">
        <property type="entry name" value="Acetyltransf_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13878">
        <property type="entry name" value="zf-C2H2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="N-acetyltransferase ESCO zinc-finger" evidence="11">
        <location>
            <begin position="146"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase ESCO acetyl-transferase" evidence="12">
        <location>
            <begin position="341"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4365644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="411" mass="45589" checksum="FCFDFF93DA998DC6" modified="2023-11-08" version="1">MPSLAAKKIVRTYSRQAKRPLYDEEPPTKRRRVETSFESSIIQVKTRPAFSSPTKSSSPTRESSEPPTSSPKDSSAIFSDELPRSSPASSPAPRSSPPPLQKRRPVFSFLKKQPKLKTPTKKPLTERSHNVQSPPEQPQKKKRMVQMQLDLATEHRKTCKTCGMEYIPSNTEDATMHRKFHAMNLGGVDFTKPLVERFRKTQVWSGGDGSFITVIGRKDALALRNRASDVLKVVNTELAAVPISNEELWSQAGITSFADAPKETTALIPEASAIEESGSPLVDRFKMYLYIRGNKCVGACLAERIWESFAVLDQNDASEQACQLPAASHSSSISISTESDPAILGISRIWTSSQHRKQGIATKLLESARIGFLYGLKIEKEQVAFSQPTESGGNLARRWFGSQAGWHVYID</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJG5</accession>
    <name>A0A9W9CJG5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJG5"/>
    <dbReference type="OrthoDB" id="5315820at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="206"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="286" mass="30114" checksum="763F9530CDE17B9E" modified="2023-11-08" version="1">MATNAPTLINLPPPPSDLDTPNEAPGTPNSTTTSMSELSTVAIKDGHRGHFPHHRNPAEAERADRISRLAGLERVAVSGRTPQGLAPGSAANPPPGYFDANNQPQIFRERSTVGSASATGSVGGRTTWASGSDVYEPDRMSEDQDVETSSMGGFSDEAASLVGFGEGARTPARQHSQIGSPAIGKASAVPGHLREQLPGSPMTGVSAPSSSGTTQTTSSVEQQKNDAKNLDGMTYDENIIDTANRTPPVATQGRDGDSTSEHAIRERMRQRQAGPEVMDYEQPKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJL8</accession>
    <name>A0A9W9CJL8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ipa protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008493</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJL8"/>
    <dbReference type="OrthoDB" id="2922289at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40788">
        <property type="entry name" value="CLR5 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40788:SF1">
        <property type="entry name" value="IPA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="574"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="593"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="714" mass="80598" checksum="9E799BF8BC7674EF" modified="2023-11-08" version="1">MDETVRELHCDLKRKFQRHGSRVEQMWRSLDQEQRKKVLIDGSRDGLVLKDPLDTSLGNVYKFLPEINLRDITSPSSDFLLDILKHRATTALHIQYTVGVSGGPGDHAHIVDMMQRKNLQLVNASRYKDCYTLFYNEEKYGQSFKTTPNHKDEVLATLMPAIQAQLIVPQAIGDLILMRQMYLLQHLNIVIEDILDTASSTRARTERPKNPTDVATAALAKLSLHSPSEKLELSHLVDTSLDRKSALEDNINLISTEPTVLAHEVNLWFFTRPELVADEKGRRLHVYTDKYISGAVFDAIHGTVKAATVWNYIARLLALLKGSSEKQFRVIVLQELSNTCHLEYTRAQALFKRNVSACSGGNKWFKRMSTVRKDGIVRISMKRNPESLTVENPQLHYMLRLCQGDTQWFKASQWLQKLEDLHRAHPLEKDKMFGGEYDSLGDLVGIVTFIQSLSSVVQLPPVAQKAGQLFVSRYSALEDELRQMKTGVDLGDFAIPIDNLLEPGMADGALTTLNQYILEKTGTKLGYLYQDLVDDCVSNINKRYEQQKAKAGEAKAEAEYMVPTAPEMPESCIQQRRQKEKTRPAHSSLYGITPQEASANTTTTTSEQPAQPQQIFEVKAATFAVFSSLLSRSSAARGSISWDAFVAAMTDLGFSVVPKGGSIYTFVPPEKVPVQRELTLHRPHQSCIEGRHLLIYSRRLKKVYGWNDETFLET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-04-02" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJP3</accession>
    <name>A0A9W9CJP3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SEC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008463</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2157641at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    <dbReference type="PANTHER" id="PTHR10663:SF373">
        <property type="entry name" value="PH AND SEC7 DOMAIN-CONTAINING PROTEIN C11E3.11C"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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            <begin position="809"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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            <begin position="50"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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        </location>
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        </location>
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        </location>
    </feature>
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        </location>
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            <begin position="1167"/>
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        <location>
            <begin position="1593"/>
            <end position="1616"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1623" mass="177501" checksum="C58D89446200775D" modified="2023-11-08" version="1">MQQTMQDFLREDSRPTTPTGPSRAHKPSVPMRSLNTNQPKQETFFDDATPVDRTRGNDQEHDLLRDSHDLSFADGATVRDSVVDNMLLSLDQFSTGNMFSNTGPQYTNYGEDDFFLRDNSYRPPGPRHRGHTYTSSRSSDYDLHPEAYAAQHSRTRRSNSSNNIGASISRKGSVRDAYPGRSTNNGPYGQLPQAGHLRGGGKKGSKGSGSSSMDFGQTGLLGTQRLGFGKRSASFDHSNANDRARLPVKVESVLDRGRMAYQNYPDDYDAAPEPTIPAGPRRAQEQPPQSPIAYPPHPSYAPPQAPGPRRRGSVTSNTSYKTVRKNKSQPETNMRAQAQEFVNASTLRDLPPVPSFQDPTAPSPTVATRKSLFPSQSQTAPPKEKPGFFRRVFGGGSSKTQAQPTTISNVTNNSQMESVPATAKQRPPEVDSIYSQARPRTTPNNNNNNHIATQLKSLPKPPQTASGPQRELQQPAPPSLAKKPSSFFRRRKKSMSENTKPPVMALDFTPPPKTVLPPQPSPGVSSLRKVMHPYLNDAELAGEKFYDSQEQISGEDKSNDERPQGFSPGYKPHKDATVRTVKSVSRGDDKTPPSSRSEQLKALQLISPDRNSPKLKLKVKHGKATAHSPQEDTFLADSSSCNEDPSGCATPTGERSGVDQTRRPSTGPTSSSLNQSASEGRNDGKEKSGRRSERLSPHNPATAGSMSASPSTSEVEDEGWVITEPTKKEQKSIGKTSAAAKRVWLDTTLPENKHGDTTDDLKLPLEGARSSQQSLDKPSPITPDATSPTSPNEVFHSATSLPIVQVESRESDAMPAIVEDRSLHTEPTDADRERAFQIYNGDDSSILKAQAAAVLGDVTLTSMRTRKAFMDLFDWTGFNILAAMRDMCGKLVLKAETQQVDRILMSLSERWCECNSSHGFKAVDVVHTICYSILLLNTDLHLADIESRMTRSQFVRNTLPTVTRVCQDAVKASGGETLRPQSTQFRRGSLPWNDRSEPNSPNADTTSFLPEAGEEPSQDKRTRSRLSIRPPARSGSEGLLSYDSATSEANILVNSPYNGPMRGWEFQVESVLKEFYDSIRKQRLPLHGVSDLAVHQQPSSNNLSVSGMLRRTPSVLSKAPSDNASYRGRSQTDFRSVGSRWANKNRSKQRLYPSSTVASSRTSLDDGSVWSPAGSSSWSRYSYGKTGTSMSMDSLGSHFAAGDYQQAIGFANALSQAIIREEGMTIASDEEFSRVAPLLEDETLELAGAPWAKEGILKHKHHLESVDKKAKDRAWTECFAVVEKGYMRLFSFSMNSKSVRNKSKLRPSAGSVVGGGNWMDNAEALDSFPLRQTIASALPPPGYSKSRPHVFALSLPTGAVHLFQVGTPDIVREFVLTANYWSARLSKEPLTGGVSSMEYGWGENVINPALIRQDSTPSVQGHMPRPSVTSSLRSSMDHATGTPKARLPGDKIVLSDWSPPTSSMMASNLMEVDQLRALTDYVKTIEGELAHHNELRAPLLITFSPRHPNAAKAMANWERKSQYLLREIVKFRTYIDTLTNAQNLKQKIYAEREAHERKISDADSAAAAAAAAQSLKPAAAAEEDGAKEAPIKASQPVVAPQSSPSTTTTTTTTTTTSAFLLHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJQ4</accession>
    <name>A0A9W9CJQ4_9PLEO</name>
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        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007785</name>
    </gene>
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        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
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            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366150.1"/>
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        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
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        <property type="entry name" value="VOC_core"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
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    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="128" mass="13983" checksum="539ED8055D778A0C" modified="2023-11-08" version="1">MSIRGDRTTDRAETENAPIFKIYDSKLHKVKIAWLGTGNSVGFEVFEFIDPPHQPVPEFEYARSGFFHIAVTSPDVDGAVNRVLENGGSQVGETVSMGGTEKAAYVSDPWGNVVELLSCSFEALMANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJS1</accession>
    <name>A0A9W9CJS1_9PLEO</name>
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        </submittedName>
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        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
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            <taxon>Dikarya</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366388.1"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
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        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ4366388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="234" mass="26197" checksum="731A348A293483D0" modified="2023-11-08" version="1">MELTALTLINVQTLLISISVVALSIVNFYFTGHALQTFGKYFPPGSYDWQNPGQPWTQPKGPAHEVQLRYDYSPENMILISTAFSILAGLFGIAGFWIARNSPKLSTFWSTSTLSASTAAFVATFISIIVSSVKYESLANSTCKWSEGRFSNPQLTCTRELVACELINFLTDTNWADNRRVCRETKGARVILVPLTVLTFVLVALYALRIHMLKTVKSVDDSAEQRVERLQREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKW3</accession>
    <name>A0A9W9CKW3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NUP160 middle TPR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKW3"/>
    <dbReference type="OrthoDB" id="67716at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021717">
        <property type="entry name" value="Nucleoporin_Nup160"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056535">
        <property type="entry name" value="TPR_NUP160_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21286">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21286:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23354">
        <property type="entry name" value="TPR_NUP160_120_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NUP160 middle TPR" evidence="1">
        <location>
            <begin position="3"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="164" mass="18482" checksum="6E44E4120ACDD685" modified="2023-11-08" version="1">MGKFLKFPLVGLADDVDAILSSLCHKTLNLASGPPYHQMLYSLRISRNNFRGAASILFERLQRLKTTSSKIHDPADDSLTQCYLMMINTLSSVRQEDAYILAEQKVDGVAPPQWGIGQGKKLLKRQIITLETLRKEYQAELDRVAAIESGQFPFVDPRDDMDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL81</accession>
    <name>A0A9W9CL81_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006682</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL81"/>
    <dbReference type="OrthoDB" id="3944408at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054505">
        <property type="entry name" value="Myb_DNA-bind_8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22980">
        <property type="entry name" value="Myb_DNA-bind_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like DNA-binding" evidence="2">
        <location>
            <begin position="5"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like DNA-binding" evidence="2">
        <location>
            <begin position="60"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="115"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="353" mass="37790" checksum="E55D5767908AB785" modified="2023-11-08" version="1">MPTEAENIQYLYLVLTNDGPPSINWDAVGTALDLKKGAVTKRWSRLKQAMEKGEAPGGSTYQFLWLCVKHSTRDKALNWPDIAAKCNTTAGAASKRYSRMKQAFDQGVAAPGSSPTKNTPTKPKATPRSTPKKTPTKAFTASGYDDGEDDDDDDVEATPAPKRKRASPKKKTPALDEQEFKEFKLEPEGEDDDEDAVSSEADKKVNKRAKVGGKAKATPKGTGKRGGNAKTTDVAAAAIQQDYDIPTPTTEATTLIKPEHEDSHMGGDVDADAVADDTFFDAHEAPLGDDLMEDLLASRSGSTDDNRKYSSPPHSRSISPLVLPPASEWAAEWSDLVGRIGNLSSGGPTGGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLY5</accession>
    <name>A0A9W9CLY5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_005762</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLY5"/>
    <dbReference type="OrthoDB" id="337038at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05380">
        <property type="entry name" value="CAP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040937904" description="SCP domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="163"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="120"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4369998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="330" mass="34763" checksum="949B3B9E688A0C55" modified="2023-11-08" version="1">MLTSLLLTSVLVMGAMAMPQSYRRKGHTVTETEVVVTTLTVFVTEPYAEPTPTSSTSLHLSTSAPLEPTSTLVVSAPAYTPDPLPASTSSATGNLAPQSVPEPTSFEFVQSSSSIPTPTPAVVSQPQPQSQPSFAHDALRAASTSAATHITGTNEAYLSSGADYRAAVLYHHNAARANHNAAPLTWDSSCEANARITAQTCNFVHYIPQGAGQGQNLFTISGKAFNVTAGITENWYKGELEPMMPYFGQSNIPDNVFHEVGHLTQLVWKATTKVGCVSIDCGSKMIVGGQSSTMNKYTVCNYAPAGNVGGRYAVNVAAPDSLTSLGSWAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMQ3</accession>
    <name>A0A9W9CMQ3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7709 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMQ3"/>
    <dbReference type="OrthoDB" id="2359405at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056126">
        <property type="entry name" value="DUF7709"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24813">
        <property type="entry name" value="DUF7709"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7709" evidence="1">
        <location>
            <begin position="9"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="112" mass="12169" checksum="7834356A4EE83A2B" modified="2023-11-08" version="1">MSQTEGTNDKLAALNSATMGTTMPVVTLPDGQKVQTGTVGALIINIKLYDELMSQSNVDNERKAELESMMAASLPVLKKANMFNLFTPEEWMEGTSPGRRFVGELALQSDQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMU5</accession>
    <name>A0A9W9CMU5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rieske domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_004346</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMU5"/>
    <dbReference type="OrthoDB" id="429143at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03477">
        <property type="entry name" value="Rieske_YhfW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.102.10.10:FF:000014">
        <property type="entry name" value="Oxidoreductase, FAD dependent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038010">
        <property type="entry name" value="YhfW_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF281">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="5">
        <location>
            <begin position="455"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4371130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="553" mass="60992" checksum="D2674D0638B38390" modified="2023-11-08" version="1">MATTTMSAPEKFMYTSGDTDPVWVHLAPYKDLPKFSRLDRDLETDVCVVGAGIAGISVAYELVTRGLNVVLVEGREVLSGETGRTSGHLASDLDDGYPAIAKKHGDKGAKAAADSHQWAINRVGEISKQLGIECEYRQLPAYDLSQYPRGAKEHADDIKTLIEEGRKAKELGLKTEYQEGFLPKGWNSSVDHRDAVIYSGQATFHPTKYCIGVLEFLKQQPNFSCYTNTRVMDVSESGIEILGLGHKEVKVQTIDGYTIKCADAVEATCVPLQKLSVIAEEEYFRTYCIAIRIPKDSIEDCLFYDSAEIYKYVRFTECDEKDVYMVVGGGDHKVGQADTTKPFQELEEWTRARFPQATTIDYKWSGQVFEPVDHMAFIGKNQGNKHIYIVTGDSGNGLTHGVLAGKLITDSITDQPNPWISAYDPKRLASIAKSLPSMLTHDLQINAQYKRFAQTDITDIEDLVPGEGGVLNKNPTKPLAVYKDESGKVHKLSALCPHLKGVVCWNHTEKSWDCPVHGSRFSKDGVQLCGPAKAGLQAVDESGEAAQKSAVDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNB5</accession>
    <name>A0A9W9CNB5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calmodulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CDC31</name>
        <name evidence="7" type="ORF">N0V83_004845</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNB5"/>
    <dbReference type="OrthoDB" id="343296at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000172">
        <property type="entry name" value="Cell division control protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF48">
        <property type="entry name" value="CENTRIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="82"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="179"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="215"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4371625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="247" mass="27413" checksum="C16EBAAC35E53FEF" modified="2023-11-08" version="1">MATPSGPLGPGPRYTLNRTQPHNPPQPSTFSTSNNNTPAAFAPSKTAQRLESERLERDRKAREERQKMDAAGQSSLAELSEEQREEIGEAFNLFDLDKDGHIDYHELKVAMKALGFELPKHEILAILNTHGVPAPSSTAAGKQPAARQASREQYQPPARQLLSFQSFQTLMAQRIVSRDPQEEIVRAFELFDEGGKGKITLQDLTRVARELGEGLSHDELVAMIEEFDMDGDNAISRDEFINICLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNE6</accession>
    <name>A0A9W9CNE6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004679</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNE6"/>
    <dbReference type="OrthoDB" id="3800839at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="332"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="658" mass="71923" checksum="72BD1B6DB37473BB" modified="2023-11-08" version="1">MEPFHRTLSNMNLASADTENYSADLHGKAGDDTPEASKSHAGRGMVRAFSASRALQQVMGFQEPDPFVPPPAVPEDLEERFVEGLGEYGLDYMTGVGMPPQETVREAGKSKLPDIVEEAESPVSDTTTHEDAVGEKIVTKQNSQFKIPMSPLVAQAVMGSGPQVEVVSRTSPPPVSYESDGANDTDIGETTSGQLGTRVHHGFDSSTSLAETPLSYPRGSAAASGAFTEAVHAHCASANTQETTNAAQWSAFAPMPLFPSPRVEQPSEDFSPIQPVISGAHARQIIPTTQEDVIKSSFAVRQKPTVPSIIVEAPSPRNKNKKKKPKSRKRAPSTTTPELSEPLPTRVRAERTTGLPTLPLASEALWKFKFPGNTHALLTILLTWQLKMQSCYNRHPNPKSFAVNPAFPYAVTPPVYQRLVSVSFYDTSVEPHKEIRFLGPGDAAEMIYAEVDTFRDEEDIEEFEEQQKQDDRLAAVKRALGLDTGYEKGSRQMAMHHRAKTGEGRWAYILVKGNKTGGPNDTAPQLMVAWHISAVTASSTCLHSIFPDDHPGFAAPAPVPAPPLKRSVSFQNLLSGARNPIRMYKSLHSTSTLELPKVDLVDTAAHDGAQTLHRTVLRMGKAGRVPLIEGFRVDVQAFRGGWRRWGGEGKVMMWRQRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNX8</accession>
    <name>A0A9W9CNX8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I and III subunit RPAC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RPC40</name>
        <name evidence="9" type="ORF">N0V83_004248</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNX8"/>
    <dbReference type="OrthoDB" id="270173at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055029">
        <property type="term" value="C:nuclear DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07032">
        <property type="entry name" value="RNAP_I_II_AC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.120.12:FF:000003">
        <property type="entry name" value="Dna-directed rna polymerases i and iii subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001514">
        <property type="entry name" value="DNA-dir_RNA_pol_30-40kDasu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033901">
        <property type="entry name" value="RNAPI/III_AC40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001988">
        <property type="entry name" value="PRK00783.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF13">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00446">
        <property type="entry name" value="RNA_POL_D_30KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="8">
        <location>
            <begin position="61"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4372474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="374" mass="42316" checksum="CA40FDBE49474106" modified="2023-11-08" version="1">MSTPSADELMRRKKLDVGPETVSNQASTDFPGHWPGENNEWDLDYFKETFKVDFHSHTPNDTSFSLISLDTSVANAFRRILLAEIPTLAIEEVFIYQNTSIIQDEVLAHRLGLIPLCGSHSGLNWMKWYAKPTDDNEGSGNPSDYNTVVLRLDVECKWQEGGLQRAVAGETDPDKLYVNHNVYAKDLKWEPLGKQSEMFPPGEEIRSTNPDILIVKMRPGQSVSLSMYAVKGIGQDHAKFSPVATASYRLMPTIDILKPIVGADAKKFARCFPRGVIKLDSVTSEDVEQHAELESKEGESKAVVDNAMNDTVSRECLRHPEFKDKVKLGRIRDHFIFRIESTGQWNSDQLFLESVKLLRVKCQRIKRGLDEMMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNY1</accession>
    <name>A0A9W9CNY1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Coatomer subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">SEC21</name>
        <name evidence="18" type="ORF">N0V83_002539</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Oligomeric complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Cytoplasmic vesicle</location>
            <location evidence="13">COPI-coated vesicle membrane</location>
            <topology evidence="13">Peripheral membrane protein</topology>
            <orientation evidence="13">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the COPG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNY1"/>
    <dbReference type="OrthoDB" id="1074925at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000046">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000071">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1480:FF:000001">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000008">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1480">
        <property type="entry name" value="Coatomer, gamma subunit, appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009028">
        <property type="entry name" value="Coatomer/calthrin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032154">
        <property type="entry name" value="Coatomer_g_Cpla"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017106">
        <property type="entry name" value="Coatomer_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013040">
        <property type="entry name" value="Coatomer_gsu_app_Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037067">
        <property type="entry name" value="Coatomer_gsu_app_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261:SF0">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16381">
        <property type="entry name" value="Coatomer_g_Cpla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08752">
        <property type="entry name" value="COP-gamma_platf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037093">
        <property type="entry name" value="Coatomer_gamma_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55711">
        <property type="entry name" value="Subdomain of clathrin and coatomer appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 13" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="15">
        <location>
            <begin position="18"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer gamma subunit appendage Ig-like subdomain" evidence="16">
        <location>
            <begin position="649"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer subunit gamma C-terminal" evidence="17">
        <location>
            <begin position="803"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="608"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="608"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="620"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4373800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="916" mass="99768" checksum="59B85771AFC8AC24" modified="2023-11-08" version="1">MDKKHEDVGGVAQIDKTSVFQEARVFNQSPISPRKCRVILTKLALLLFTGENWGRQEATTLFFGISKLFQNKDASLRQMVYLVIKELAGSADDVIMVTSSIMKDTSVGSDVVYRPNAIRALCRVIDASTVQAIERLVKTCIVDKNPSVASAALVSSYHLLPVAKDVVRRWQSEAAEAASGAKSGGGFLGGFGGSSHTALQASTNYMTQYHAIGLLYQMRSGDRMSLVKMVQQYSAAGVVKSPAATVLLVRLAAKLAEEDPNLRKPMMQLLDGWLRHKSEMVNFEAAKAICDMRDVTDGELVQAVHVLQLFLTSPRAVTKFAALRILSQMASFKPDAVRSCNQDIESLITNSNRSIATFAITTLLKTGNESSVDRLMKQITGFMAEITDEFKVTIVEAVRTLALKFKAKQSGFLAFLSGILRDEGGYEFKRAVVEAIMDLIRFVPEAKEDALATLCEFIEDCEFTKLAVRILFVLGREGPSTPHPTKYIRYIYNRVVLENAIVRAAATSALAKFGVGQKDPEIKKSVHVLLTRCLDDVDDEVRDRAALNLSLMDQADDDMAVSFIRNDSMFSLPVLEHQLAMYVSSDSRDTFESAFDITKIPTVSREQADAADLTKKTEGATPTLKAPSAAKAPSKVGADAAASAASNAQKYAVELQKIPELAAHGGVLKSSSVVELTESETEYVVTAIKHIFKDHVVLQYDIKNTLPDTVLLDVEMVVTPEDDGDVQLEEEFVIPAPKLPTNEPGTVYVSFKRLETESQFISASFTNVLKFTSKEIDPSTGEPEDGDGYPDEYQVEDLDLNGADYVVPAYAGSFDNVWDQSNGDSATETLQLSNMKSISDATEQLTNTLSLQPLEGTDVAISASTHTLKLYGKTITGGKVAAMIRMAFSAKTGVTMKIDVRSEEEGVAALVVGSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP06</accession>
    <name>A0A9W9CP06_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_002780</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP06"/>
    <dbReference type="OrthoDB" id="2687058at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050794">
        <property type="entry name" value="CPA2_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32468">
        <property type="entry name" value="CATION/H + ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32468:SF0">
        <property type="entry name" value="K(+)_H(+) ANTIPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="421"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="9">
        <location>
            <begin position="50"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="517"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4374041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="891" mass="95192" checksum="D80781CEF0071BE2" modified="2023-11-08" version="1">MSSVVSSASASPTAPIKAPPQGGVLEGVNPVHFDAKNPIILFIVQAGIILIFCRALNWPLSKIRQPRVISEIIGGIILGPSVMGRIPGFRASIFPDASMTNLNLVANIGLTLFLFIIGLEVDLRFLLSNWKVALNVGLASMAIPFGLGCAIAVGLYNEFKDEPGTVQIDFSIFMLFIGVAMAITAFPVLCRILTELKLLMTPVGVIVLAAGVGNDVVGWVLLALCVALVNAGSGLTALWVLLTCAGYMLFLVYIVRPAFIYVLRRSRALQDGPSQGIISLTLLIALGSAFFTGIIGVHPIFGAFMAGLICPHEGGFAIKVAEKIEDLIGGLFLPLYFTLSGLNTNIGLLDSGIVWAYVVGVVAVAFFSKFISATLAARGTKMLWRECFAIGSLMSCKGLVELIVLNIGLNAKILSTRTFTIFVVMALVTTFASSPLTMYFYPMWYQKKVESWRRGEIDWDTGKPLDGSESTRNSVHYDKIAAEKIKRLTVYLRLDSMPNLLAFTSLFGGDSAKPAPKTHPAKVIDASSKESSQPSTKGDAPNRPVEAYGLRLLNLTDRGSSVMQVSEIDSYTAYDPVVNTFRTFGRLHNLAVSGEVLVVPESSFAETLSTRASDSDLLLLPWSETGGMSEQTIIEDKGTKNKLVASSYNSFVNSTLEQSVIPVVVLINKNFGGSKNKDQKQSLKLTRKFSNVSLTSARDKAITAPIADRSHHIFFPFFGGADDRAALRLVLQLAENAQVTATIIHFDVPESIIGSTLVPATSVGTSTSKAGEITSTPLSSSREQDAALFASLHASLPADLSNRVVFETVASTTPVQDVIARAGIEVGQTPKNAGDLVVLGRNIGRNSVLAMDDDVDAIRSSSSAGSTLGVLAEKVWEGKLGASVLVVKAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP13</accession>
    <name>A0A9W9CP13_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Translocator protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_004204</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TspO/BZRP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP13"/>
    <dbReference type="OrthoDB" id="8841220at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033013">
        <property type="term" value="P:tetrapyrrole metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15904">
        <property type="entry name" value="TSPO_MBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.100:FF:000001">
        <property type="entry name" value="translocator protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.100">
        <property type="entry name" value="TspO/MBR protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038330">
        <property type="entry name" value="TspO/MBR-related_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004307">
        <property type="entry name" value="TspO_MBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10057">
        <property type="entry name" value="PERIPHERAL-TYPE BENZODIAZEPINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10057:SF0">
        <property type="entry name" value="TRANSLOCATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03073">
        <property type="entry name" value="TspO_MBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4372430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="19008" checksum="1E09427B2E78679D" modified="2023-11-08" version="1">MTTYLPSLTLPSFVFENPAEDAAKLPIATSTQATYHSLKQPPLHPPGYVFGPVWTALYATMGYTAYRAYTIGASSMNPETAALAKHGATLYTLQLFLNLAWMPLYFGLGQPIAATADILALGGTLAYLTKVWGQVDPVCGWLLAPYLGWVGFATYLCVGSGYLNNWDFKNAPKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPA9</accession>
    <name>A0A9W9CPA9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fatty acid desaturase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003097</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPA9"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36459:SF1">
        <property type="entry name" value="FATTY ACID DESATURASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36459">
        <property type="entry name" value="ORF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fatty acid desaturase" evidence="2">
        <location>
            <begin position="151"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="449" mass="52746" checksum="C48A6AFD71FE3913" modified="2023-11-08" version="1">MGRLPFPADAELTGPDRVVLGILQKQYEAYGDPIKNASSEAAEESDDSATKHSEFVSPSPEDLQDQEQQAIAYLKKLNNPKSAGFEPTVFSSVDEPDIKNPLLRQWIIDPYIRWARTAVRVETDVIMVTHLLLYSCTTIPSALYLFNNFHWWHAIIHGVMQGWYMGAYTLLRHQHIHMRGILSKKYAIVDEFFPYFLDPFMGHTWNSYYYHHVKHHHVEGNGPDDLSSTVRYQRDDVWDFMRYVGRFYFLIWFDLPRYFLRTRKPGLALRSGGWEVSNYVFLYCMFTYVNARATVFTLLVPLALMRLALMTGNWGQHALVDEVEPDSDFRSSITLIDVPSNRYCYNDGYHTSHHLNPLRHWRDHPVAFLSQKKQYADEHALVFYNIDYMFLTINLLRKNYEHVAKCLVPMGDQMKLSLEERVEMLKRKTRKFTEEEIAEKWGKQYAKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ26</accession>
    <name>A0A9W9CQ26_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Xaa-Pro dipeptidyl-peptidase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002890</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ26"/>
    <dbReference type="OrthoDB" id="416441at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02231">
        <property type="entry name" value="cupin_BLL6423-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3020.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005674">
        <property type="entry name" value="CocE/Ser_esterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000383">
        <property type="entry name" value="Xaa-Pro-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013736">
        <property type="entry name" value="Xaa-Pro_dipept_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050585">
        <property type="entry name" value="Xaa-Pro_dipeptidyl-ppase/CocE"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00976">
        <property type="entry name" value="CocE_NonD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43056:SF10">
        <property type="entry name" value="COCE_NOND FAMILY, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G00600)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43056">
        <property type="entry name" value="PEPTIDASE S9 PROLYL OLIGOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02129">
        <property type="entry name" value="Peptidase_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08530">
        <property type="entry name" value="PepX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00939">
        <property type="entry name" value="PepX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xaa-Pro dipeptidyl-peptidase C-terminal" evidence="2">
        <location>
            <begin position="501"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="776" mass="87937" checksum="858C7B64430F4258" modified="2023-11-08" version="1">MASRNGIENNFPDVQRFITTHDPSTKEAIFANTVSEKTKVDRIPGAAFRLGYVTKGFPVELNENADLAVYKSYLESPPGLVASGGTVLRFVDMEPGSLSPMHRTVSLDYGVVLEGEVELVLDSGETRKMKRGDVAIQRGTMHAWRNLSSTSWARMMYVLQECKPVEVDGQVLKEDYGTMEGLEVKDAHNVDESSFPYIYEENVAVPLKTSTRSIIRCNVYRPRSTSEGAKLPVLVTYGPYGKDIHYKDFHGKSYSEVNSEHHSDHSAWETPDPGFWTKHDYIIVRADERGLGQSPGFLDTMSRGTSEAFFDVVEWAADQSWSSGKVGLLGISYYAGSQWRVAARKPKGLAAVIPWEGMSDYYRDRCRHGGILSNKFIDFWWNRQVITNQYGRPGRAERNWGPDTVDGNLSEEIREKNRQDQTIDTAKFKFRDEQYYASKEYDMSDIEVPLLSVGNWGGILLHLRGNIEGYTHAGSEFKYLRMITGRHDLPFYYKEEVEIQRSFLDAFLKGDDRVGWSIKGKLPPVDMVLRKGNVGFNDSEKERAYERRTENEWPIARTRYTDFYLTPSQSLTQARPITNRPQKLSYEALGTLDDPKLLQFTTSAFEEETEITGHIFAHLNVSLSPHAAGSTPSDIDLFLTLRYISPEGKEVHYTGTAGDPVPLTKGWLRVSCRKTNPDHAKHREHLPWRDYFSTNVQPVIPGDVYAVDVEIWPTNVVVEKGGKIIFEISSGDTQGSGIFQHNHPEDRSQEKFAGLNQLHFGEKFVNYVTLPIIPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQH2</accession>
    <name>A0A9W9CQH2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001535</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQH2"/>
    <dbReference type="OrthoDB" id="5383057at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="224" mass="22716" checksum="AF2AEF3EA2EAE2D4" modified="2023-11-08" version="1">MPTSHGQKVTTDPESTRNPIQEGVGTVTSDSLAADSIKGGGSFGEGNPKAAASKQPSASTTTNTTDTSNTKKLDPAVDAHAREAQEGWSEEKSYNSSKGLGKESGVGPTYNDVGSGVNSSGDARGVSTGTSGGSDNVQGNVAPTGAYAGQDLDSGVLKPKGKNVTEDPELKGNSKFGEIGTDQDPARVAELEFAKRDAAPAAVPNRDLAQDGDSKFSALKDEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQJ8</accession>
    <name>A0A9W9CQJ8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-dehydropantoate 2-reductase</fullName>
            <ecNumber evidence="4">1.1.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ketopantoate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_002043</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16233"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.169"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.169" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQJ8"/>
    <dbReference type="OrthoDB" id="3609at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008677">
        <property type="term" value="F:2-dehydropantoate 2-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000017">
        <property type="entry name" value="2-dehydropantoate 2-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003710">
        <property type="entry name" value="ApbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051402">
        <property type="entry name" value="KPR-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00745">
        <property type="entry name" value="apbA_panE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708:SF30">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708">
        <property type="entry name" value="PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 4" id="KW-0521">NADP</keyword>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="10"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase N-terminal" evidence="6">
        <location>
            <begin position="11"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="7">
        <location>
            <begin position="189"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4374964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="325" mass="35373" checksum="6535F0D2E6E1B9A1" modified="2023-11-08" version="1">MTESNSQPRVLLFGAGGVGAVYLYLLSKVSSTTAVCRSNYDVVKKDGFTINSSIFGKNIYFRPNVVRDCEEAVSSGPKPYDYIIVCSKAIPGVIPQLIKPAVTLGQTAIVLVQNGVGIEQEYHDAFPDNPIISAVVYLPATQRPAGVIKHGEVEKLQIGSYPSSAPNERASAFTKLVQSAGATAEFYEDVQPKRWFKLLVNASWNPICALTLSKDTDVLGSLDEASGVLLATMLEIRDIAAAYGYTITRDEVEYQLGRAQARIPTNAGIEPSMLQDVQAGRRIEVEAIVGNAVRMGKEKGVACVRLEMLYVLAKALDLQIERRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR87</accession>
    <name>A0A9W9CR87_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000565</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR87"/>
    <dbReference type="OrthoDB" id="1046782at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF143">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C X DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="439" mass="48083" checksum="6C8901303288D192" modified="2023-11-08" version="1">MGFGGCIGIINASPFTWTQTAISSYQFIQWDNSFPSIIKPGEVYKNYIERDTALFVTESDTVGKVSFTLDNPSNTQFEFVAGENNIFVTPTNFDQAGATHDNNQINVGTFLHDQCVTFYLSGEQGSLYANNPPVDWMHSTLDVIGNKTLQDIAIPGSHDAGIGPDVEYGTFGVNENNVKTQQLDIGGQLAQGVRWFDIRPVVGDGGKWLTGHYTGGSGSLGANGESIEKIISQVNTFLSASSELVILELSHAYNTDADYRNLNQDEWNTLFDMLTTNVKYLYTKDVSDLSTIKISDFISGGPAVLVVARPDDDISLGKYSAAGIHPDSSFKVFNSYADSSNVDNVVNDQLNKMTNWKTMDPSKNVFLLSWTETEVGIDNAFGNGIESNAGEINERLFGNVAPVLSGNNRPNIIMEDYIETNELASLSMAINWIQANIWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSE6</accession>
    <name>A0A9W9CSE6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Palmitoyltransferase swf1</fullName>
            <ecNumber evidence="2">2.3.1.225</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SWF1_1</name>
        <name evidence="2" type="ORF">N0V83_000852</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSE6"/>
    <dbReference type="OrthoDB" id="3797485at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4378022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="115" mass="12108" checksum="FACFC609821A4936" modified="2023-11-08" version="1">MHRYWLLSRTCADKKTDHIGGGLNLNVLGALSGALSSKSKKTTHQNPDGSSDTTEDRHDKAAANGIASGQGNAYAAGGAQEHSLKSKEKGVGQEATQAKQLKGKKEEVNHLGIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXM7</accession>
    <name>A0A9W8XXM7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010721</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXM7"/>
    <dbReference type="OrthoDB" id="21418at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38645">
        <property type="entry name" value="CHROMOSOME 9, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38645:SF1">
        <property type="entry name" value="YALI0F12243P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="252" mass="27954" checksum="55858438B9DD3E13" modified="2023-11-08" version="1">MDSMRSLNKSLPKTRRQPDVHQSFRTAALNVTNLYKSALADIDRARSDGYQEALDELLSFLDKENMGVGDGEGWRIRQWATERLDGVLPKHSSSDSDDEYLDDKRARSSSPIMDRNLSHEDTRTLEPVVEAQRSDSAPPPLQMEATTTEADATPLHHVFQFAAPQAYPSGTTNDNTTPDVSAAARRAFPTPRRSSNRSGPRNLQRSAAQNLFQLGNGAGQKRRLMQDFFNIDGFNDRRDGSSGGGSKRGRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY40</accession>
    <name>A0A9W8XY40_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010041</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY40"/>
    <dbReference type="OrthoDB" id="3892429at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="351"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="417"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="434" mass="48374" checksum="C36B28D207EBE0BA" modified="2023-11-08" version="1">MSPAPIIQADAAASLSPPQQAAPPCAATPIPTHFDDIFHDKFMHIISEWKNDPARGQLTQKERKRFIETAFLETDELYFGKRQLSDQEIKLRALTKVFYKVLAEEPWSEDWIRSPDVERKLSKFEMHKDSVALAAEHGLVTPRDAVKAAREASLKHEKALKDIKDPKALKDMKINPGPYDEIEIIYIAGTVSSTATNRIDRENIYPVSVSSVANVLQNHICRDMVRPFSMAERLVNCSDTKELEDMRRFACNVCDFAPHLLDNDNVSLLVVLLILSRSECCRRFENNGIRIEGSTISHRKAECMKNKMGWKTSEEKKPYDNFANEFQTRVKNACFPAPRPIRLAGRKPPRKSSTPATTPATPATPSTPPVYPPPPMGYMASPQVRNDSVNTGINIPAPSMQSIYSQTAAPAVKTQRARASTGAGSSAVEPMDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYX1</accession>
    <name>A0A9W8XYX1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Maltose/galactoside acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009760</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYX1"/>
    <dbReference type="OrthoDB" id="25818at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03357">
        <property type="entry name" value="LbH_MAT_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051159">
        <property type="entry name" value="Hexapeptide_acetyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024688">
        <property type="entry name" value="Mac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416:SF54">
        <property type="entry name" value="ACETYLTRANSFERASE, CYSE_LACA_LPXA_NODL FAMILY (AFU_ORTHOLOGUE AFUA_2G08430)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416">
        <property type="entry name" value="SIALIC ACID SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12464">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01266">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Maltose/galactoside acetyltransferase" evidence="3">
        <location>
            <begin position="7"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4363466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="217" mass="23792" checksum="9ADF5801B37B3E3A" modified="2023-11-08" version="1">MSLSENKQRMARGELYHAFTPELIAERARCKHAVARYNNAGEVPRRKLTELFRDIIQDKTPLPPPAATSEDDDKLFEHDPWIEPPLAMDYGYNVRLGENVFINFNSVFLDTCLITVGSRTLVGPNVSFYSGTHPLDPAIRNGTQGPELGKEIHIGEDCWIGGNVCILPGVTIGRGSVVGAGSVVTKSVPDFTVVAGNPAKFIRKVVTDMDPSQKVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZB5</accession>
    <name>A0A9W8XZB5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZB5"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="61" mass="7233" checksum="0C67C87C10223F68" modified="2023-11-08" version="1">MGKINHSKSKSKKKKKKKKKKKKKKKKKKKKKKKRDEIDAATEAFMYSSISEISEYTISAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZD3</accession>
    <name>A0A9W8XZD3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Metalloendopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">OMA1</name>
        <name evidence="8" type="ORF">N0V83_009959</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZD3"/>
    <dbReference type="OrthoDB" id="7464992at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07331">
        <property type="entry name" value="M48C_Oma1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051156">
        <property type="entry name" value="Mito/Outer_Membr_Metalloprot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22726">
        <property type="entry name" value="METALLOENDOPEPTIDASE OMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22726:SF1">
        <property type="entry name" value="METALLOENDOPEPTIDASE OMA1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M48" evidence="7">
        <location>
            <begin position="141"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4363663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="348" mass="39409" checksum="0667152B3582E619" modified="2023-11-08" version="1">MFRIRPPLRAFQIPVRTRQPTSRPSQTQFQPLRSHAQRRYYEQQSFGRGPRYQRFMPAGRLYGLLVRWYGRPTFYRDVGIISAGAGGIYIYNLEEVPVSGRRRFNIISPGMEEMMGKATVDQVREEYQGKILPDYDPRVRSVKKVLQRLLPFAEGEGLKELEWEVAVIDSPEQNAFVAPGGKVFVFTGILPLCEDENGIAAVLGHEIAHVVAHHTAERMSQAPLILLGCIALSMFDVSFYSGKMLLDLFLSMPASRKHEAEADFIGLMMMAQGCYRPEAAMDFWARMEKAGKGAPPEILSTHPSNHNREEKIREWLPKAQEKAEESDCSMTGKFANDFTSALGGFGRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZI4</accession>
    <name>A0A9W8XZI4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZI4"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="292"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="414"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="440" mass="48019" checksum="69868B51BA2E73B2" modified="2023-11-08" version="1">MDTTFITIHDLSDDANILYSSDSIVDILGHTPDEVVNRSVWQFFHPEELPFAKAQHSRGVKLDKAAVLSYCRLKNRQGDWIGCECCFSVVYDVMVCCTSIYRNGMQSQKRAVEAPIVRKLFASSPKDPRYHMLSHLSSKFDLGPEQQTHEPRAALFLNRFTRTLTVMYATSGIEQIIGISGEAMKGRSFYYCIAPNCLEDAVRCLESAKGNDSIAYMRFFFRDPRQDDPPEVTPSDSEDDIMTDVTGSDDESEGGVEIDGPSTSESTSSRNLSHGSNSMDNSEEPPSNARTNSGDSNNSSDTHQAMFGGRSAHHSSTSSIASSRSERGNDPIELEAVISCAQHPAMVQAGPSLQFQNAFMQSIRDVAVFAWALTGINGSLADYARGSPNGESQPPEGFPVWNPLPDPTQYGRHSAAPSGYVSGSSSSSNDPFGFGDPGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZQ7</accession>
    <name>A0A9W8XZQ7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">INO80 complex subunit F domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_009785</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZQ7"/>
    <dbReference type="OrthoDB" id="10070927at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056513">
        <property type="entry name" value="INO80F"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24245">
        <property type="entry name" value="INO80F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INO80 complex subunit F" evidence="4">
        <location>
            <begin position="22"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="74"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="179"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="120"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="202"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="223"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4363490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="251" mass="27549" checksum="955F10B5765EB3CF" modified="2023-11-08" version="1">MPQPPARAFSQKDLPPSVESAYYRKCIDLRRRINDIEENNDGTRLRITRLNRGIQKMRLERAFLLEQLNKHMEYNVDDSDRSSSPPPTPTDKPLRSKRSHNRKGTPPAGAQVGGLPGGMQHQSPGSTHHQSLLHQMNPMSSAQSTPDPSRQPNSYFSTVGAAATSPHAVNGAAVLPPLHSLPPLHPQQQSAQRAAYFDPAYDEQRPTPVDHEGDGGRRRAYSGAQQPSAETAPPQNGDTEMTDAGFTAVNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1A7</accession>
    <name>A0A9W8Y1A7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008861</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1A7"/>
    <dbReference type="OrthoDB" id="4776947at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040772824" evidence="2">
        <location>
            <begin position="21"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="157" mass="15198" checksum="B752858785A608DB" modified="2023-11-08" version="1">MLAKTIFAATFLALAQFAVATPPGCLLGAVNQYDDPSDVASICKEKDLSSTVASYCGDDAKTALAALADICNDQGVKVATDVPTSTSVSSKPSGTGSYTIYPTGSGSASGNSTAPTATGGPKASGTSTGGLAESTGAAGRIEIGVAAVVAGLMAIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1G2</accession>
    <name>A0A9W8Y1G2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_009459</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1G2"/>
    <dbReference type="OrthoDB" id="2985014at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF21">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4364004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="364" mass="40104" checksum="086F71E301D324BD" modified="2023-11-08" version="1">MAKRQLGWGAATLAHAFIQGRSALIALRLMIGALEAGFYPTAVAYLSYFYCRYDMAVRVGLFFGQYAIAGAFSGSIAYGVFHLRGSRLHNWQYLFIIEGSLTMVIATIAWFWLPQGPGSAWFLNAEERKFAVERITKDSAKYVAYEGGSGEQRLTRRDVVETAKDWKLWYVLVFNICASVPSQAFSVFLPLVVEGLGYSSIEANLVPSKERGYHIIGGICIALIGLITTVTVHSSGAKYAGLCILLLGSYVSPPLTVVWLSGNTPEPGKRFLVLGVNGFGNLAGVIGSQLYKRRYAPTYRLPSYVTLGFVAVALVGYVAYRFTLKAVNRKKLAILAGKSTQEVEGERVDPTRYADKKWTFIYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1R1</accession>
    <name>A0A9W8Y1R1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NuA4 histone acetyltransferase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ARP4</name>
        <name evidence="3" type="ORF">N0V83_008154</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1R1"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13395">
        <property type="entry name" value="ASKHA_NBD_Arp4_ACTL6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="348"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="352"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="477" mass="51535" checksum="21C5B4DFA5068E9B" modified="2023-11-08" version="1">MNIPSSSAAPAAGEYAGDEVSAIVLDPGYSSVRAGFAGEDVPKSVCPSFYGQNSAGEHLFGENAIHAPVPGLEIKNYWGADGIVEDWDTAAKLWEYAITSRLTGARPGDPAKNGLNDGTNANGDANMDVEMEGTENNEAPLEDSPLLVTEPGWNSAKAREKYIEIAMESWGTPAFFLQKTGVLAAFASGKASGIIIDVGASHTSVTPVLDGMVLRKGVQKSHLAGNFVSSQIRQIFKQATPEIPLTPHYLVKSKTQVDAGSPAQATYKSFANPLTSSFRLNEEDRILTEFKESVVEVWPGPGRLNNGGTNEELAKNMPGRPFEMPDGWNQVFSSERFRAAEGLFDATAALPTSTSNSNSDSTNTTTRPKDDQTIPRLVQAAVQQVDADQRPMLLSNIVVTGGTTLLYKFNERLNYEINNLYPSTRNKLIAPGSVVERKYAAWIGGSILASLGSFHQLWISKKEYEEHGSGIVEKRCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1S0</accession>
    <name>A0A9W8Y1S0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">P-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">N0V83_008605</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family. BCS1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4364989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1S0"/>
    <dbReference type="OrthoDB" id="10251412at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057495">
        <property type="entry name" value="AAA_lid_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014851">
        <property type="entry name" value="BCS1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050747">
        <property type="entry name" value="Mitochondrial_chaperone_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23070">
        <property type="entry name" value="BCS1 AAA-TYPE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25426">
        <property type="entry name" value="AAA_lid_BCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08740">
        <property type="entry name" value="BCS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01024">
        <property type="entry name" value="BCS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="BCS1 N-terminal" evidence="15">
        <location>
            <begin position="32"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="14">
        <location>
            <begin position="267"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="506"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4364989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="554" mass="60718" checksum="AC7A1CD89D1CA5AC" modified="2023-11-08" version="1">MKRSATASFPILEVIQRLLYRLKPSGGDLEKILALIGLYQAVRPVYGHLKDFCIWACTVQVTIPENDPVAKEVLAWMGSKVILNSRTRNAMLVTGGMQDPNDDFHRRMIMRGPGAIEKRVDDEVLCLPPIGTRLFWVGYRPFIFARRGGGSFRSTSVMSGNLVDDSGQLQNSLSLLTLGWSLKPLQEFTALCHQFKLDNLTGTTTVYFAGGGHGDPYGDGWQSVSKAIRKLDTIDMEEAVKSDLIRDAEYYYSDQSRQFFADCGIPYRRGYLFYGPPGTGKSSFSAALAGHLGCDIYHINLATGDITDGGLHRLFLGLPRKCIVVIEDIDSAGIGREQGPSLRPNAATQDLYDNHPGLLPNVPPPSRARGERRRNMVTLSGLLNAIDGNASQEGRLLIMTSNNPDALDAALTRPGRIDKKVYFGNMSRTAGKSIFKRLIGRSALAHDAAYTMTQVEEYATEFADKVPANTFTPAQVQNFLQGCRGDPIKALRDIDAWVEENRDSVGMATARTSDASEIIVSRPTSADAAQSPLEDGSEDSDSESPKPRQPQSMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1S2</accession>
    <name>A0A9W8Y1S2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1S2"/>
    <dbReference type="OrthoDB" id="341259at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052391">
        <property type="entry name" value="E3_Ligase-Neurotoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24133">
        <property type="entry name" value="ANKYRIN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24133:SF40">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="369"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="413"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="501" mass="56797" checksum="20433B453BF0B765" modified="2023-11-08" version="1">MQLLHLSPELLKPIIDNVIKREGIRGAVHYRLVCKTFGSYIMDSCIGSASKLAFASLRKDSVYKHLLHHYGGAILSKKVLRSGEKLQDKDKIYSENLQLSSFAQQLVTYILEQQWSSIQGREKLRDGYTLAICNVVMTLDTEAFQECLIGIYQPEAGELWSLKKMEPALAAAVGDMDTLRRTLTLPGLLTPHYNLFPGILHAAIASNQTGVLNFVLDFVLTNVRGKRESGSWDEMRDHARAISDALKMAIQHHKNHAATMILEFISNFDVLEGSLTLHFEDNLIKHSMQFGNIEFLDKALAYCSIFRSMEKEFVYKNPNILVSASQIGNAFRTGHSQVLRQLLDYGKLDPNHFDYYWDERQRKVHRLLANETPLEAAMNGHRYDLARVLLTHGADVNGTAQLKKIDKCKTGGPGKTALYYAADRGDIQAVQFLLAHKADPVVSKEDNTKSPRHVASQSRWRKCAFLLDIAAREGNEATLRSDTWRQYKDYLDSQREDYPYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2I8</accession>
    <name>A0A9W8Y2I8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4</fullName>
            <ecNumber evidence="6">3.1.13.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Carbon catabolite repressor protein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Cytoplasmic deadenylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Glucose-repressible alcohol dehydrogenase transcriptional effector</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">CCR4_1</name>
        <name evidence="26" type="ORF">N0V83_008383</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="26">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="23">Acts as a catalytic component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. Ccr4 has 3'-5' RNase activity with a strong preference for polyadenylated substrates and also low exonuclease activity towards single-stranded DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CCR4/nocturin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2I8"/>
    <dbReference type="OrthoDB" id="428734at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000447">
        <property type="entry name" value="Glucose-repressible alcohol dehydrogenase transcriptional effector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF100">
        <property type="entry name" value="POLY(A)-SPECIFIC RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="12 26" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="17" id="KW-0804">Transcription</keyword>
    <keyword evidence="16" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="25">
        <location>
            <begin position="385"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="19"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="124"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="179"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="24">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="124"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="193"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00030493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00031469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00033317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00045495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAJ4365763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="484" mass="55063" checksum="238DEE758F90242F" modified="2023-11-08" version="1">MAEYSVYQRGAGQTFYPQHAQSIRNLPNRVRSPTTSARIPFNNPDTPSPSRSPVRSSPQQFAMFNQGHGHQSHNVMMNGRGGQQNYAMQMNLGKPFQHNQHNQHHHQHHQHHQEQTGINHGAQYNHQHNISSGGMSSVQPNFTPSHLQNGTPGSVNSGLSKPPNEHWAEQLRMAQIAREMTQSHSHARIHPSVSKSIVAGTSNGMQKDADKEERNRPAAQRVEDAKEHHIWTILDFGGQNLKVITPALFNYTFLTKLYVNCNKLTYIHQAVGQLRNLTHLDLSLNNLQYLPPEIGMLVNLKQLLLFDNHIDNLPYELGSLYQLEMLGIEGNPIPDDLKQIIIEQGTTELIKHFRENAPGPEAPPERDWIVLDEIQDPAQETVTALSYNILCDKYCTQSQYGYTPSTALAWENRRELILAELRERNADIVCLQEIDQDSFNEFFREALAHNDYKGVFWPKSRARTMAEREAKLVDGCAIFFKNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y355</accession>
    <name>A0A9W8Y355_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_007331</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y355"/>
    <dbReference type="OrthoDB" id="2441642at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF51">
        <property type="entry name" value="QUINIDINE RESISTANCE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4366806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="251" mass="27817" checksum="F4465985145F3059" modified="2023-11-08" version="1">MQMPNPLKSLLIVADFETLLALLPAAVSSSVLVHQLLSFQQHPGRPHVRFSVLTTGKFVDKSYRRHAKKLGVEIEQDRRQDLTEFPVERARLELTIPIFILLSAFVIIYGWIMPHHANQAGPVIVLLVLGYCMIAVYQVLSVLLVDLHPRQAATASAANNLLRCELGAAFAAFISPMIDGIGTGWSYTEMALITVIMIPGLFLTMIRGIRWRKSKAAKQEAKKSKVENSTSVNAKGWGIDSDVSRLPVSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3K6</accession>
    <name>A0A9W8Y3K6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fungal N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007898</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3K6"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="31847" checksum="D1AC80D6D7517617" modified="2023-11-08" version="1">MEVLAGVASGISVVSLSIQLIDSVNEIRKLIRNVKGASVELARLAELLTRLAAMLEDVRNLSALRILGMRQRKSIRYMSTLRSQQDRLLSSHQEEIISEENEYILNSKLFGLGIRWSYEGAYNRILPSLSVYPVVPKFSVDVYTAFLDADLQDIQKMFASGALHPFTQEQCSDQSLLHEESGDWSLRWEWWYEAYGTKPPADTVFSQYEVLATCWDRWEWPFAQWKFRSTTEEMRIESKLDARFQRRMAAKARKERARAGLKRPRSKIPGSWVFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3M2</accession>
    <name>A0A9W8Y3M2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Shugoshin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_008773</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the shugoshin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3M2"/>
    <dbReference type="OrthoDB" id="5394106at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000779">
        <property type="term" value="C:condensed chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045132">
        <property type="term" value="P:meiotic chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011515">
        <property type="entry name" value="Shugoshin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011516">
        <property type="entry name" value="Shugoshin_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07557">
        <property type="entry name" value="Shugoshin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07558">
        <property type="entry name" value="Shugoshin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shugoshin N-terminal coiled-coil" evidence="12">
        <location>
            <begin position="21"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Shugoshin C-terminal" evidence="11">
        <location>
            <begin position="435"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="163"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="557"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="29"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="404"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="457"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="517"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="568"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="586"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="610"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="671"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="729"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4365155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="757" mass="83018" checksum="768710FB3EF61664" modified="2023-11-08" version="1">MARLNEPPIAPQPSAETIDAVKRRFLRQNRELAKTNSQQSIRIRSLENDCSRLLAENLSLREQVLQLHNNIESRPAFDHIDAVKTQLEAKIQELGGLVAGLAKIKRGDEKGRRKSLVAKRRSGGERQWRSGLGLQEVENGMMPTITEDKYYPRRTMGADELQEALDDPDSQSPDIGPPPVSRFENEEPIAFNPSPASEEQQGGIDEEGEPALSVNLETRKKRRESGPKLNIRRVSLFESPEEAEEGATKAIRTGAKRKFSVQEDADKLQSNGEPFRFSRRNTPSASETEASNEDSRPSSPERPVLGSSKRLNCLIITTNTNVAEPVNTDPVVSPKKQRSSVQEKPEKNSQPLSKPRGRSRLNITRNIAQPDFPPLQMPEPIQTAEIILDSLPPKTPAVEEIFSPPSTEPSTQRPESKDTPPPGDLSSSDQIGQAARPGRRSRPQVSYKEPSLHTKMRRPDAKLVDAVIDRRTSAEPQTAPSTAIKRESTGDLNWKPVSAVSGRGGEEEVEVGSPLRQKLDRRDIGHDSKTTPLPVEQEERSTASKAISALINETSIAKRKAAAPTVSVVDTKSASETLPVNSQEHSAPKEEPTIKQEAPEKDSLAVFDFTDSSPAEPSNNNPRTRINELAKAARNARRHSAMPAPTASVTFESRRADKAEGALPSLHKRTGSGNVKSNSTTSLGKSTTMARSSSVKERKPTSSSGGAVAARADTIDVKMKSDIPAAQEEGARVRERERETSSLRAERAASRRKSMML</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3T3</accession>
    <name>A0A9W8Y3T3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcription initiation factor TFIID subunit 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TAF9</name>
        <name evidence="7" type="ORF">N0V83_007766</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3T3"/>
    <dbReference type="OrthoDB" id="341924at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07979">
        <property type="entry name" value="HFD_TAF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000069">
        <property type="entry name" value="Transcription initiation factor TFIID subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003162">
        <property type="entry name" value="TFIID-31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051431">
        <property type="entry name" value="TFIID_subunit_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48068">
        <property type="entry name" value="TAF9 RNA POLYMERASE II, TATA BOX-BINDING PROTEIN (TBP)-ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48068:SF4">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN ASSOCIATED FACTOR 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02291">
        <property type="entry name" value="TFIID-31kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="15"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="59"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4366131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="252" mass="26628" checksum="9B6FF036EF9B9C23" modified="2023-11-08" version="1">MASPAAETMQNGISTPPTTTNANATPPTQSSTQPNTQQASQLSNGAAAQPPPPSQPPSMNASLPSTSLSDTGASKRPRDARLIHLILANMGVHAYTERVPLQLLDFAYRYTSGILSDAISYEPPAHASTTSKKRGADKGGDDDGGGVSLNALRTAVGARAASTFTAQLGKEFMTEIASERNRIALPRVEREFGVRLPPERYCFTGVGWDVKNAWEGDEVSEDEEMDLGDEAGGSGVPGLEGRWVVLARMETG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3V7</accession>
    <name>A0A9W8Y3V7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cation-transporting ATPase</fullName>
            <ecNumber evidence="13">7.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">N0V83_007696</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.-" evidence="13"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="48943at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015662">
        <property type="term" value="F:P-type ion transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07542">
        <property type="entry name" value="P-type_ATPase_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000032">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000119">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000057">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000068">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047819">
        <property type="entry name" value="P5A-ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047821">
        <property type="entry name" value="P5B-type_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF8">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12409">
        <property type="entry name" value="P5-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="564"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="600"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1110"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1134"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1174"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1221"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1251"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1288"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="domain" description="P5B-type ATPase N-terminal" evidence="16">
        <location>
            <begin position="181"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="15">
        <location>
            <begin position="417"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="18"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="123"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="739"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF12409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4367166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1346" mass="150916" checksum="F4FCCDDB7CC7E35D" modified="2023-11-08" version="1">MSEASFMEDVEMAQDEVFELGPMPLSRSYSGASRSRIPRRKPGQQLTPTIQVFSGPMSESVPTSVSAFSHRARHRADSSASLTSFAYYDEDQDSDEGLEDGAIIEDGESEYDGYTVEELEDLEAGLSSQSTSPSPSRRKSSSSRKSVDRPLLRRHSSTGSAGSGADWRGRRSNQKIYIMTEDLTIVIAGFKTSPIGFAVYALLCIATFGIGWLLFRWLPRWRVALVGRATPLKECDWIVIENQWGEFAVQDVSRQTYGRSLSTVFGDPEKGRHGDWDEDEDPLIKELRCLDYRYIRFVYHPIKDKFVLANTWKDPAWTEVSALREGLDNDERDYRELVFGKNLIDIAEKTIGQLLVDEVFHPFYVFQIASLILWSLDEYYYYACAIFIISAVSIITTLLETKASMKRLREVSKFECEVRVVRSGFWTHVDSAELVPGDVYEVTDPALTQFPCDSLLLSGDCIVNESMLTGESIPVSKIPVTNHSLDLLDLSASAVHPEIARHMLFSGTKIIRARRPHEDHVDDEAAALAMVVRTGFNTTKGALVRSMLFPKPSGFKFYRDSFRYISVMAIIAMIGFVASFINFVHLGLAWHLIVVRALDLITIVVPPALPATLTIGTSFALSRLKQKQIFCISPQRVNVGGKLDVVCFDKTGTLTEEGLDVLGVRVVERPRNRFSELLADPFDILPGSQHDRDPTVEYLAHKTILYTMATCHSLRKIDDELVGDPLDVKMFDFTGWSYEEGEQKSGNSGDDDPEQKLSPSVARPPPGREYDVDDREDDPNRKPVELGVLKAFEFVSQLRRASVIVRQFGSKSGQVYVKGAPEVMKEICRPESFPTDYEELLAFYTHRGFRVIACATKTIAKLNWLKVQKMKREEAESQLDFVGFIIFENKLKPSTAPVIEELERANIRKTMCTGDNILTAISVARECGLINKTAHCFVPHFVDGDSRTALSKLSWESVDDPVFKLDENTLKPLPPPPEADVSLPYDVSNLRNYSLAVSGDVFRWIIDFASESVLREMLVCGQVFARMSPDEKHELVEKLQSIDYCVGFCGDGANDCGALKAADVGISLSEAEASVAAPFTSRNFDISCVPQVIKEGRAALVTSFSCFKYMSLYSAIQFCSVSFLYASASNLGDFQFLFIDLLLILPIAIFMGWSGAYATLSRKRPTANLVSRKVLTPLLGQMAFCILIQFIAWHFVQNQPWYLPPVIDKNHSNSLNSQNTALFLVSCFQYILSAVVLSVGKPFREPMSRNLPFITTIFFTLAVTAYMLFDPAAWVMQWMELTYMDARFKVFIAALGLGNFAVAYVSERLLFPGLAKWIGVLKVRVGGERGRKKRKAYKVIAESMRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3X4</accession>
    <name>A0A9W8Y3X4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_008030</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3X4"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45768">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF13-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45768:SF18">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL47-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="25"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="123"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="124"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4366394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="163" mass="18137" checksum="4F769FFCCA3BF16A" modified="2023-11-08" version="1">MFATSEAFIDGGLDFSLGSDAEGDCSICADTNMSELVQVKLCGHEFHMGCLKEWLHLSNTCPLCRVSLFKPYEESDREIFTEMLAENVVLSFQTLSDIALRGHVPEATELLRHLIVRLFFSSTGDDDNDSDDGDGNDSDDDDEHDSEDEQSNSEDDLPDDFVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y489</accession>
    <name>A0A9W8Y489_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_007791</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y489"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF41">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="375"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="442"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="59"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4366156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="502" mass="55498" checksum="00A0416E203895E5" modified="2023-11-08" version="1">MTIHDDVPKLDVEQSTDEKHVLNNQHVDKAAQFLVEVDAYPPLSPEAERKLIRKVDWIMIPMLFITATLGAVDKVALGTAAIYGLRTDAHLVGQEYSWLGSILSLGALAGMFPSSYLIHRLPSAKYLCACSVGWSAMALMMVACTNWSSLMAVRFFMGCFEAIIVPGISLIIASFYRKSEQPPRNALVFAAASSIVNGFLSWAVGHIDAKLAIWQYLFLIVGSVTFAWSLFVLWYMPATPMNAKFLSQEEIYHMVRRVAENKTGVENKEWKWYQVKEAFLDPKTWIIFFFNIAINIPNGGLTTFNGIIISNLGFSSVNASLLSMPTGVMSTLSAFIFSYIGAKWSNRRCLVTVAACIVPIIGTAVVYAVDRKNVAGQMIGIYLLYTYFGPYVVGIGLAQANTAGQTKKTVVFAILYIGYAVGNLIGPQTFRANQAPAYTGGVVAMIVCYCACIALICLYWALCVFKNKRVGSEAERRMSVADCMDDLKDQTDFEQEGFRYTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y493</accession>
    <name>A0A9W8Y493_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DDHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006981</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y493"/>
    <dbReference type="OrthoDB" id="69269at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004177">
        <property type="entry name" value="DDHD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058055">
        <property type="entry name" value="PA-PLA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509">
        <property type="entry name" value="PA-PL1 PHOSPHOLIPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509:SF6">
        <property type="entry name" value="PHOSPHOLIPASE C1020.13C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02862">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01127">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51043">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDHD" evidence="2">
        <location>
            <begin position="714"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="484"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="937" mass="103417" checksum="F84866A41E2F067F" modified="2023-11-08" version="1">MAGNEPNASSHANQYIREVLHSTEAPPTIPVRYFYTSPLAIDDPLSPLPPPPTGTTSGKRQPPRPFSEYDNDAIQESWLELRKKILKHNEELGEKEGKGPGTPTNPAVARLKRKRGGSTSSDEASIQGRDIPGTRSPSRGQYSRSAELVDRRRPGSSGGSQGPTVMSGSLKAVDPGQSSPLEPTTTGTPFTRLPSRGNIAKSWKPAPTEGGHRPAIHEQDSYKWDDDMEPALPGLKDKGPAKIPSKPKVGPSAKVPVGVSRLHHVVMDADSIRMEPIYWSPLSDVAQIVRGTWFYKDSMMPVEVEVANMLEAGYLEMRPWTQTWKDELNSAVEVGAFGEMKILHNLWPEKPKKAESRPSTSQQMQAGLVQSTLPEGEDDPEKERREIVENVCDLIDISTGPNGPDHKAAGDLEYGADGRKRLYLKAGVVYAKDNEAYILKPTLQPSDYYGRRPLANYIRKGRSIGVCVVRGFDQAIWDRLHPSKNTPKAQAAREGVSSSQAGAPQYRRQKSDPELAQSERPKVTDLVLVIHGIGQKLSERMETFHFTHAMNAFRREVNVELGTSEVKRHLRKDMGGIMVLPVNWRHRVSLEVDAANDAEVEDPDTNKYTLKDITPETLPSVRGIVSDVMLDIPYYLSPMHNPKMISACIQEANRIYRLWCSNNPGFAEYGRVHLVAHSLGSVMAIDILSQQPTYVDPRLSDPSLPEDDLPNDQFIFNTTDLFVCGSPVSLFLLMKNANLLPRRDKEKPGADSYAAPGVAGEQGTYGCLSVDNIYNIINPYDPVASRINPTVDVVYASMLKPATIPSASSSYFAFVGNPFRRAPSSSSANNHPDNKPSTLRLPSNVELETHNFTREEVAEKRAFLLNDNGQIDYFMKYGGGPLEIQYLTMLGAHSSYWLSRDFIRMIVVEVGRTVGKEGTVPAMRAQKKKVIVGGGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4T4</accession>
    <name>A0A9W8Y4T4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase S54 rhomboid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_006414</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4T4"/>
    <dbReference type="OrthoDB" id="10260614at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050925">
        <property type="entry name" value="Rhomboid_protease_S54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731:SF14">
        <property type="entry name" value="PRESENILIN-ASSOCIATED RHOMBOID-LIKE PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731">
        <property type="entry name" value="RHOMBOID PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="387"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="9">
        <location>
            <begin position="304"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="14"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="138"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="41"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="56"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4368059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="569" mass="64290" checksum="F60CA7655EDAC982" modified="2023-11-08" version="1">MRFINRVTQLWGSTPTIVGTRRTVQDSEEEDTSTDKRIEPENTELEDPKAEDEESKFEGFDDEESRHAESKDADLKIEDPREIEDQAEGSELDLPDTGPEAILVDGDSGGRESEAIEISSASSLTAYRPALCQLRNQYPTRGQHNQPPRPPRPSGPQSSQQEVRHRADQEKEQILYAANDGQYQPEPEPVFEDFPKVKVRYLRPAVWALAVSGGIYTGLAYLQAKEELKPKKPAAWLQAPQWSTPRRTPPTPTEIATGWWDQMNPISRLSIGIIATNSAVHASSFLVPRFWDTLWHLPARNVNYTQFTSMFVHSGALHFFVNMYFLNNFMTPVGYSRLFEGSPYHVLSFFLTTGVLSGFAQHWATLIPVQKRAIPEIFIRCGGASGALFGVLGVFCMQYPTAGLGILFVPVHFDAQYVLPAIMLFDFIGMVRGYSFVNFGHAVSTIRFDPSKCLALTTQGTFVRSADWRGVFLFRREDEPLEATCTILEAPVTRHVVSPEAPEYPINCLYMHRYQYVPIDKSSHLRQTSQHFLPYCIMLSTQHPPALYQNPSRHQKIRKVRRDASTASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5D7</accession>
    <name>A0A9W8Y5D7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_006612</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the OSBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5D7"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008142">
        <property type="term" value="F:oxysterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120009">
        <property type="term" value="P:intermembrane lipid transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000010">
        <property type="entry name" value="Oxysterol-binding protein homolog 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF92">
        <property type="entry name" value="OXYSTEROL BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="383"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="506"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="392"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="405"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="428"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="517"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4368256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="553" mass="61373" checksum="20D18D1C3B4C57C9" modified="2023-11-08" version="1">MSNDIASNRSTLKEFLASIATISGDLSNITAPPFVLAENSTVEIPQYWADHPLLFVAPAAEPSPEKRALLVLKWFVGSLRNQQYAGRREDEGVKKPLNAFLGELFFGSWQDAELGETRLVSEQVSHHPPVTACYLWNDKHGIRAEGFTQQEITFSGSVSIKQKGYAVLHIDKYEEDYLVPVPNVKVKGIISGVPYPELHGDYSLISSTGYVSHVKFEGKSLFGSGQKNGFEARLFHVDHPKEDIYTVNGAWNGSSTIHDSKSEKQIETFDVMTLQSQKLHVSDVSDQDPWESRRAWGDTIAALHSSDMKGTTDSKSVIEEGQRQMRKDEESQGKQWKNIFFRSVQQDPIFEKLAVHDNGSFTIDREGGIWKVDHEAITTAKKPYHGVLLPSNEKHNSTEREGSRNENNVQNQSQNTNVVAGTPSEAPQEQKQDVVKGRIPETNNVTHEQRASTAEEEQRAKGVPEGAEGTANEGASTWQNLTNNVNTTVSNLNEQLRGVTQAASAYVSGEQTTTEAVQEKQPEKRTSNLREATDAEVEAFLRAKHSSNNRGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5L5</accession>
    <name>A0A9W8Y5L5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_007652</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5L5"/>
    <dbReference type="OrthoDB" id="1939598at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14705">
        <property type="entry name" value="bZIP_Zip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13044">
        <property type="entry name" value="ACTIVATING TRANSCRIPTION FACTOR ATF 4/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13044:SF14">
        <property type="entry name" value="CRYPTOCEPHAL, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="113"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="116"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="182"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4367122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="201" mass="23424" checksum="586E0D06E1B77CDA" modified="2023-11-08" version="1">MTRKYRSNTQSDSRRFISLLFSFSFIFLMKCQRRLTRPRGLQYTSPTMAGHMPQQYEHESAIQVLEELQGFSPITYNISAYRTPYPDSNATSTSSATSPDPANAQIITDSVIVEADEDKRKRNQAASARFRQKKKQREQQLMETSREMQERTRKLEAENDGLKKENMFLKKLLVEKVDHMSDEDREMLKKATEGTLNVKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5M1</accession>
    <name>A0A9W8Y5M1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_007304</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5M1"/>
    <dbReference type="OrthoDB" id="408373at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43329">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="177"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4366779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="439" mass="49260" checksum="77F888E3F3E7EB9D" modified="2023-11-08" version="1">MSGQFKRANPLTYLNEVDLPAMRQGLIKFQTSLTSLTINTMDSSWQVDLDIDIPGFGSFREFVVLRYLDVSGLVLWGDSIDEEYPRLSTMLPESLEILKVKTEWDHDVDDAFCNLIEDGRAPSITSHVSSIQLPTNNPMDASRHRQRIATITTTSSEQIRLNYIECSHPASNQRKGVILLIHGFPQTSYQFRHVITPLSDAGYQVIAPDYRGAGQSSKPLTGYEKTQMAEDLHILVRTHLNITEKTHVVGHDIGGMIAFAYASRYPTDVASLIWGECPLPGSSCYERIKGTSDVFHFVFHQVPDLPEALIAGKERAYCKHFFDKIIYNSGAITPADLDHYALAYEQPGAIRAALEVYRAFERDAEENREWRGRNGKMEVPSLLLMGGKFMLAESAGEMASEFHEGAEVEVVAESGHYIAEENPDAFVKGVLKFVRKHRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5S3</accession>
    <name>A0A9W8Y5S3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-type proton ATPase subunit G</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_007946</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the V-ATPase G subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5S3"/>
    <dbReference type="OrthoDB" id="250802at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2950:FF:000009">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.620:FF:000004">
        <property type="entry name" value="V-type proton ATPase subunit G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005124">
        <property type="entry name" value="V-ATPase_G"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01147">
        <property type="entry name" value="V_ATP_synt_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12713:SF11">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12713">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03179">
        <property type="entry name" value="V-ATPase_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="32"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4366310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="116" mass="13302" checksum="C39637851CC763AE" modified="2023-11-08" version="1">MSAQNSAGIQTLLDAERDAQKIVQQAREYRTKRVKDARNEAQKEIEEYRKEKENEYQKFEKEHSSGNQKAEEDAKKDTDAKVKEIDVLGKKSGSKVVDQLLEAVINAKPEPPRKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5S9</accession>
    <name>A0A9W8Y5S9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006846</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5S9"/>
    <dbReference type="OrthoDB" id="4457531at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4367265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="355" mass="39699" checksum="BC5ABAEFCF44422B" modified="2023-11-08" version="1">MSSPAIGRIGDQEFAGLSLDELDENEFQRLARAGLPLLLPWHREPVRLGTGYHSSRQLTKDPWAGETPFILAELMLQAKIFRPEQGTTSSFTSKKTSRSVETNDHLTLGFGVGVSPPIPVVTVSVKGTFDQHVQENSDSDKQSIRAGCRAGCIDFESGPRLTSEAIREIKYGGGFDRFRERYGDYYVAGYRLGADTGLLLSSSTSSRKQVDKYSIKAEVEVLFFSASKTWEKDFEKFRCGRSLKLVGYDTLEGRNWKNAGAAGDAEREMNNWLQGRPTADEGSLRADAEAIIQRSHSLIDRVGEVLHTHNVRNGGYLTFDQCEDLVEAGVVVELLLLPMERLRDVFRWRLDDNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y639</accession>
    <name>A0A9W8Y639_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_005967</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y639"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140115">
        <property type="term" value="P:export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042908">
        <property type="term" value="P:xenobiotic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF24">
        <property type="entry name" value="TRANSPORTER, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="422"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="455"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="42"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="499" mass="54787" checksum="4749D4DD6CE4A8DB" modified="2023-11-08" version="1">MTLTERERRGNETEPLLPRPETAPHIVTFSPNDLEDPRNWPLSRKWLMVAAIIPIDLSVSWGASGFSPAAGDFARDVGVSPLVATLGLSMYVLGLAFGPMTLAPLSEYYGRRPVYIVSYGIFLLLLLATTYVESLGPFLVLRLFSGYFSSTTISNFGGTIADLFHHHDTGPAMSWFLWAATGGSPTGFVLFSFIAHGRSWHAVFRIMFFVCLAFWIILVGALYALGETRHSVILLRRAMTLRRVTGDEQLEVPDELKQRGPKQLFGTALARPFRFLASEAIVQFGALYNGFLYGLSFLFNGAFHMIFGPNGYGFDTIGVGICFLGIVVGISLGLLTNIFQEQHYKRQVAQAGHKDVPEARVHHAKLAAVILPVSLLAFALTATPNIHPFVPVLASAFWGWSFYTLILMTLTYTEDAYKTYSASALAGIGLVRNLAGAGFPLVGRRLFARTGTRNASLVLMAIAVCLVPIPFVLEKRGASLRKRSPWAAAHEDEDDSDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y654</accession>
    <name>A0A9W8Y654_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Beta-galactosidase</fullName>
            <ecNumber evidence="3 8">3.2.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_007028</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 35 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="3 8"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y654"/>
    <dbReference type="OrthoDB" id="1657402at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000065">
        <property type="entry name" value="Beta-galactosidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000040">
        <property type="entry name" value="Beta-galactosidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.20.10">
        <property type="entry name" value="Beta-galactosidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.390.10">
        <property type="entry name" value="Beta-galactosidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018954">
        <property type="entry name" value="Betagal_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037110">
        <property type="entry name" value="Betagal_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025972">
        <property type="entry name" value="BetaGal_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036833">
        <property type="entry name" value="BetaGal_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025300">
        <property type="entry name" value="BetaGal_jelly_roll_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031330">
        <property type="entry name" value="Gly_Hdrlase_35_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019801">
        <property type="entry name" value="Glyco_hydro_35_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001944">
        <property type="entry name" value="Glycoside_Hdrlase_35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23421">
        <property type="entry name" value="BETA-GALACTOSIDASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13364">
        <property type="entry name" value="BetaGal_ABD2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10435">
        <property type="entry name" value="BetaGal_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13363">
        <property type="entry name" value="BetaGal_dom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01301">
        <property type="entry name" value="Glyco_hydro_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00742">
        <property type="entry name" value="GLHYDRLASE35"/>
    </dbReference>
    <dbReference type="SMART" id="SM01029">
        <property type="entry name" value="BetaGal_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117100">
        <property type="entry name" value="Beta-galactosidase LacA, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01182">
        <property type="entry name" value="GLYCOSYL_HYDROL_F35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040990297" description="Beta-galactosidase" evidence="10">
        <location>
            <begin position="23"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-galactosidase" evidence="11">
        <location>
            <begin position="394"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4367445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1025" mass="111940" checksum="2EF9F3B829F44FDF" modified="2023-11-08" version="1">MKGFLKFSSAIALSCFAVEAAARAVGYSPKEWIRPYKREPLQDIVTWDEHSLFVHGERIFLYSGEVHPYRLPVPDLYLDVFQKIKALGYNGVSFYVDWALLEGKPGEYRADGVFDLQPFFDAASEAGIYLLARPGPYINAEVSGGGFPGWIQRVNGTLRTRAPDFLAATDNYMKNLGATLAENQITNGGPIILVQPENEYTQALSSITPFPDPVYMQYVEDQLRNAGIVVPFISNDASAKGNNAPGQPAAVDIYGHDGYPVGFDCQNPTVWPDGNLPTNWHQLHEQQSPSTPYAIVEFQAGSFDPWGGPGWDKCSVLVGAEFERVFYKQLYSFGVTILNLYMTYGGTNWGNLGHPGGYTSYDYAAPIREGREVDREKYSELKLQANFFKVSPEYLTAMRGNASNSTWTTSTALSVTPASGENAKFYFVRHSKYNSLESTSYKLKISTTAFGNITVPQINGTALTLNGRDSKIHVSDYTLGGATLVYSTAEIFTWHKYADKTVLVVYGGPGETHELALAVTGLEILEGDVKSISTRGYTLLNFKADGTRKVAKVGVESNFIYVYMLDRNEAYNYWAVDQAPHDSSNPVILKAGYLTRTAMVTGDTLALTGDLNATTPIEILGGAPSSLSKLTFNGDEFDFKVSKSGAVTANIDYPRPKFTVPQLNELKWKYIDSLPEVQPGYDDSEWTKADLEKTYNSLRALTTPVSLYSSDYGYHTGTLIYRGTFTAAGNETTFYLSTQGGSAFGSSAWIGDHFLGSWRGYDAALNGNNTFTLPNLTKGKTYTITVVIDNQGLDENWTIGTETMKNPRGILDYKLSGHAQSDVTWKLTGNLGGEDYLDISRGPLNEGGLFAERQGLHLPGALSASNAGWKASKGPVADGIAAPGIGFFATEFDLNLPSGYDIPLSFTFSNGTTSTTNTTSGSSYSTNNSTAPGSSVAAYRVQLYVNGWQYGKFVSNVGPQTKFPVPEGILDYRGTNYLGVSLWGLDDEETKIEGLDLSVDGLIWSAIGEVGTVEGQTYESREGAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6E1</accession>
    <name>A0A9W8Y6E1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2293 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006558</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6E1"/>
    <dbReference type="OrthoDB" id="5288828at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018744">
        <property type="entry name" value="DUF2293"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38113">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38113:SF1">
        <property type="entry name" value="DUF2293 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10056">
        <property type="entry name" value="DUF2293"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2293" evidence="2">
        <location>
            <begin position="178"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="362"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="387" mass="43928" checksum="1E534BFBEF16295E" modified="2023-11-08" version="1">MARIHHARPQHAPKRGDTLGQSRAARKEKKPYKIVLEAVTQEKKKLHSILTYGSNAPSGFGFIPAGHPEFTEWAKEQCRQRNLDVHVVSAKPKNKMHTDPAKLSHHVHRIGHHFPLEIIQLACSRFGYTYDQERGLRKVTTSGGANWIAQHVEDYSSRQPPHELPTTEEETRDYIIGAVREMFPKIPEPDVASIVDHAFAEGSTRVGNAKNLPLARKIQLAVVAHIRHTYTDYDKLLKTDGWLDARSQVEPISLAKLKEWRDETGEMSNEIEETFREVIVLDDDDNEVSSDDGTLSIPDEREQSMEIVTSRATARDMQPERSSDYAHTDIHDGRHLFSRTIVVRPYHPPPSGSTVFEPVSKYIHESRPPPPGESETLRFAHNRPVDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6E5</accession>
    <name>A0A9W8Y6E5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006283</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6E5"/>
    <dbReference type="OrthoDB" id="540004at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052356">
        <property type="entry name" value="Thiol_S-MT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45036:SF1">
        <property type="entry name" value="METHYLTRANSFERASE LIKE 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45036">
        <property type="entry name" value="METHYLTRANSFERASE LIKE 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4369198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="264" mass="29380" checksum="338FB08A684EC260" modified="2023-11-08" version="1">MTILETVFLELRPFTIFNLTKLRFKSFARLWKHNGEAMSTEMPGPTFQLLSECRGIVLDIGPGSGEIMPHFNPTMIRALYGAEPAEDLHPGLRRKAQESGFGAKFHPLLCGAEPESLIPALHKSGILDLSGKDGLASEGVFDEICCVRVLCGVPHAEQTIKGLYNLLKPGGRMVVCEHVANPWRTEGRVTARFMQLVYTVLGWPFFMGGCELQRHTMGYLRDAGEWDKFDLKYYGPTDCIPFVVGELIKEAGSLEKSYAQAVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y711</accession>
    <name>A0A9W8Y711_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006508</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y711"/>
    <dbReference type="OrthoDB" id="3889716at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="693"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="700"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="748"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="759" mass="88356" checksum="846DC80A54D4F95A" modified="2023-11-08" version="1">MSLFALLDMRSMSPSLQDSDSSREESPLFDPLNLPKPKATANLHSPLLKLPDDIFKCIMDYLDRDAAWSLKRLCKGMADSETVKQLLYKYPIQLTDVRDIRLGDWKYRGLGLLRWQYFKESINDSNRKYVHKLAMSHWASISDFLWIEDNLPSLVSLDISAIKDFVWTPEETWTWKMLAEACPKLFARLEELEVANWADYTAHSRIEYSYSYNDYRFKQKFRISRRRDGGSVANMLFPICKKLKTLAIRERYSGFHTWNEWEVHQRVCCLVDGVQKHCPASMTKLRVHDYAPYRSLFSTDATAWSRLTDVEIGLYSWMEDRRDRDVIGPIPYRITQGHHHRDEEEAFDDKTFDSCKRDHMVLGNHVVQGVGASFEDLLQSLQTISNKYPKINIRPIRNLQNITLHPFHLVNVMQRRHHFGQNTQNNDQASSDPSSKPEVQEALRWLAQTCDWKPILAWDSMMCDVFPANLEPNRTFLPKADVVSRIQNMVSTLRGLNIPIRVSIGDRTNACPSSGLDGSLYFGDYKMFVGEGEDKREVLLPTQASFSLTKIASMVDELTIQYPLDVPGVSGWLRAAKRPTPAEKALMQREMVGWRRFWARYASQFKNLKKLTANVPNDIYEDWGKGDLAALLADPRWEMLEVEERGGDFGFFGSYFPFSSSSLRYSFPRKRTRTKFVQRVFFRLDDEQLDLTALDTEMGEQEREEREITDEQIKDRELPSHRFWSTKDEEKGVKRKRLDEGTEDEEDQANKKPKVEQAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7N7</accession>
    <name>A0A9W8Y7N7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA-processing protein 42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RRP42</name>
        <name evidence="9" type="ORF">N0V83_007199</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7N7"/>
    <dbReference type="OrthoDB" id="272245at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0271">Exosome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="8">
        <location>
            <begin position="38"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="73"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="279"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="85"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="291"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4367614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="360" mass="38651" checksum="7935CD25D80E18F6" modified="2023-11-08" version="1">MAPQHALVNLSPAELSFLRSTLAQTPPIRLDVTKGPRDFRSMRAEYDVLPSANGSARIALEDGTEALVGVKAEVERSPQRPTLGEQEDVLMGDSEETDDGKGKGQNSWVEMSVEIPGFREDDALPVFLSSMLTESLLASGELKDRLYINRRFHWKIYVDILLLSPPLSYPLALLSMATHLALLTTRLPALKSEKDEDPLFDDDWDASIPLYPKNNTKVAKATQTGRPPVIILAMAVGPNIIFDPCKEELAVADAVLAIACTNSTSSPTGARIVSIRTIDPPSHLTPPGVPNSMNSATGGTVPTSSADALTQRELLAESGVWTPPRGGMKRGLISQVTKMVVEHGGVAEEVISALAAIEVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7R4</accession>
    <name>A0A9W8Y7R4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">4-aminobutyrate aminotransferase</fullName>
            <ecNumber evidence="3">2.6.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">GABA aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Gamma-amino-N-butyrate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">UGA1</name>
        <name evidence="12" type="ORF">N0V83_005338</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23352"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="2.6.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.19" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7R4"/>
    <dbReference type="OrthoDB" id="10260828at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034386">
        <property type="term" value="F:4-aminobutyrate:2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000029">
        <property type="entry name" value="4-aminobutyrate aminotransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004631">
        <property type="entry name" value="4NH2But_aminotransferase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00699">
        <property type="entry name" value="GABAtrns_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43206:SF1">
        <property type="entry name" value="4-AMINOBUTYRATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43206">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4369576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="512" mass="56673" checksum="8FEC0105CC6F441E" modified="2023-11-08" version="1">MASLLRTAGRARSVLPRTIPHATSRVAPIAFQRRHASAAAAAVADSSEQSFFPDEPTAPIIKTEIPGPESKKAVERLSKVFDTRSLNMMADYSRSYGNYIADLDGNVLLDVYAQIASIPVGYNNPALLLAATSPSMASAIINRPALGNFPQHDWADILESGILSVAPKGMNQVFTGQSGSDANELAYKAAFMWKRQQQRGGAHVDFSHEDISSSMNNQSPGAPDMSILSFKSGFHGRLFGSLSTTRSKPIHKIDIPAFDWPQAPFPSLKYPLEENEAENAKEEQRCLAETERLITEYHSPPAAIVVEPIQSEGGDNHATPAFFRGLRELTKKHDILFIVDEVQTGVGATGKFWAHEHWGLSTPPDMVTFSKKAQTAGYYFGNNDLRPNKPYRQFNTWMGDPARAILFRAIIQEIKRLDLVSSTAATGNYLFDGLQRLAERYPSEIHNLRGKGQGTFIAWDSPRRDEVLKKAKGQGVNIGGSGEQAVRLRPMLIFQRRHADLLLERLERIFQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8Q3</accession>
    <name>A0A9W8Y8Q3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_005310</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8Q3"/>
    <dbReference type="OrthoDB" id="3934656at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF168">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4370788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="25374" checksum="27BEE95417282736" modified="2023-11-08" version="1">MAKNVFSIAKKLVAERFGPSAKSHHDMLGSFIRHGLTQEEASGEALLQVVAGSDTSAGTLRAVMLNALTNPTIYSKLRAEIDENLAAGKISSPITDAEARQLPYLQATIREGLRIMPPASGAFFKTVPPAGDVIDGKFIPGGTQIGSSPLGIHRSKRIFGVDADIFRPERWIEASPEKFAEMANTVDLVFHYGKYQCLGKGVALMEFNKVFVEAGIPLQVRLQMLTMFVALQTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8R9</accession>
    <name>A0A9W8Y8R9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_004978</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. TCR/Tet family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8R9"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17502">
        <property type="entry name" value="MFS_Azr1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000014">
        <property type="entry name" value="MFS drug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF158">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G14490)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040773740" description="Major facilitator superfamily (MFS) profile domain-containing protein" evidence="8">
        <location>
            <begin position="20"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="303"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="473"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="4"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4370460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="519" mass="55631" checksum="086CACD8600E0BDD" modified="2023-11-08" version="1">MAAILIALFLSLFVAALDATIVATAAPTISRDLNSAAGYTWIGGAFLLANAASGPIWAKFSDIWGRKLIVLTALAMFFASSAVCATAKTMEALIIGRAFQGAAGGGLILLVHVCISDLFSLRQRSLFMGFTEGVWALAGGVGPVLGGVFASLVSWRWCFYINLPISGFAALLIVLFLDIQHEHTSFKAGIKAIDWFGMFTFLGCTLMILFGLDFGGVIFPWDSAKVIALLVVGGIMIFAFVYSEARMAKYPLIPMTLFKQRTNVAAFAVAFFHGFVFIAGEYYMPLFFQAVLEASPLRSGLLLLPFIVTGCVAGVLAGVVIHRTGRFREIAWIGTFFLALGFGLFISFDAYTTTAKAVGFVMIGGLGSGLLFEAPLIAVQSQVEQQDVASATATLSFVRNIGLSISTIIGGTIFQNSMAGRASFLRAAGLPEELLDKFDGDHAMANVLVPSTLENPIWELAAKQAFAYATRNMWITYTVFALLGFVASLFLEAAHLGTEHTETVTGLRKEKPQPAIESP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y911</accession>
    <name>A0A9W8Y911_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glutamine--fructose-6-phosphate transaminase (isomerizing)</fullName>
            <ecNumber evidence="4">2.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Hexosephosphate aminotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">GFA1</name>
        <name evidence="13" type="ORF">N0V83_005105</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y911"/>
    <dbReference type="OrthoDB" id="15235at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006047">
        <property type="term" value="P:UDP-N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00714">
        <property type="entry name" value="GFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000001">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000002">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047084">
        <property type="entry name" value="GFAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001484">
        <property type="entry name" value="PRK00331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 13" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="11">
        <location>
            <begin position="2"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="12">
        <location>
            <begin position="371"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="12">
        <location>
            <begin position="542"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4370584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="693" mass="77256" checksum="6C6C46E523825EA0" modified="2023-11-08" version="1">MCGIFGYINYLVEKDRKYILDTLINGLQRLEYRGYDSAGLAIDGDKKGEVYAVKEVGKVASLKKLVEEQNFDLNKVFDSHAGIAHTRWATHGPPSRVNCHPHRSDPNWEFAVVHNGIITNYKELKTLLESKGFKFETETDTEAIAKLTKYIWSEHPDLSFPALVKAVIKELQGAFGLLLKSVHYPHEVVAARKGSPLVIGVRTQKKMKVDFVDVEYNDEGTALPAESAAHNVALKKSNNLLAPPDKSLLHRSQSRAFLSDDGVPMPTEFFLSSDPASIVEHTKKVLYLEDDDIAHIHEGSLNIHRLNKDDGSSNVRTIQTLEIQLQEIMKGRFDHFMQKEIFEQPESVVNAMRGRLDAVNKTVTLGGLRQYLATIRRCRRIIFIACGTSYHSCMAVRGIFEELTEIPIAVELASDFLDRKAPVFRDDTCVFVSQSGETADSLMALRYCLERGALTVGIVNNVGSSISLLTHCGVHINAGPEIGVASTKAYTSQFVCMVMFALSLSEDRASKQKRREEIMVGLTKVSDQFKEVLKLDEPIKKLCEKFKDQKSLLLLGRGSQHATALEGALKIKEISYLHCEAVMSGELKHGVLALVDENLPIVMILTRDDIFAKSLNAYQQVIARSGRPIIICNENDPEFPTDKTDKIEVPSNVDCLQGLINVIPLQLMAYWMAVAEGLNVDMPRNLAKSVTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y918</accession>
    <name>A0A9W8Y918_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">mRNA 3' end processing factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PCF11</name>
        <name evidence="3" type="ORF">N0V83_005530</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y918"/>
    <dbReference type="OrthoDB" id="343582at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005849">
        <property type="term" value="C:mRNA cleavage factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16982">
        <property type="entry name" value="CID_Pcf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000016">
        <property type="entry name" value="mRNA cleavage factor complex component Pcf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045154">
        <property type="entry name" value="PCF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054127">
        <property type="entry name" value="Pcf11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047415">
        <property type="entry name" value="Pcf11_CID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021605">
        <property type="entry name" value="Pcf11_Clp1-ID"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921:SF3">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX 2 PROTEIN PCF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21936">
        <property type="entry name" value="Pcf11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11526">
        <property type="entry name" value="Pfc11_Clp1_ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="2">
        <location>
            <begin position="4"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="582"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="682"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="146"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="584"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="701" mass="76414" checksum="CFAF9582FBB82862" modified="2023-11-08" version="1">MSFPAAEVAADFRDALQDLRMNSRPEISNLTLIAKENTEYAEAISTELENHVRSTRPEWKLPALYVLDSIVKNVGTPYTVYIGRNLYKTFMDAYLVMAQDTRKAMEGLLRTWKQPVPESMDPRPVFPADVTKDIENALMKIHAAQQAQRPQHALPARPPTNMAWRDVSTPPPSLPRFAAPNDPRARPPFPTTPQYGPPHTATPPVPYHQPPQQAQPMSSLDLSELKSEVTQLVSTTQTMFAHNPVDGALRTKLSALLSLKQILDTQTLPPQQLEAVRQQIRALAPPAPTPVFSPPTPVYPAQNSVPLHFPPPLSPNYPPVGAPPNSAPPNVAQLLAGFRPPMHQPPQPTPPPPNPPAFNLADLLKRVSSPAQLSSVPAPAPFQPPFQGFPPPVSTPVPIAQAAAPAPTSTPTANLAALLAQFNKPAAAPPISQSTPIQSTPIPQMPPTPQPPTAAPALGSAEWLLKALGGVPGAMPSTSTPVASQPMIRQCSAPFDIMDQIELTTASMKKPRFNLISRLYEAKPNICATCGRRFESTSEGKEKKARHMDWHFKVKDPDAAKRGVHRSWYMPEKEWIEYKEVDETSPNQTSNDPNNLASTGGKLKKQAKDRYVSVPQDVVSQQAPCPICQEKFETVWSVEANDFVWMDALSVGGKIYHATCFEEYSKGAGIAMPGTPDSVLGKRRADDGSSGIGEQKKVRAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9E3</accession>
    <name>A0A9W8Y9E3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamine-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="8">6.3.5.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">NAD(+) synthase [glutamine-hydrolyzing]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_004554</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>deamido-NAD(+) + L-glutamine + ATP + H2O = L-glutamate + AMP + diphosphate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">In the C-terminal section; belongs to the NAD synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.1" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9E3"/>
    <dbReference type="OrthoDB" id="2020662at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07570">
        <property type="entry name" value="GAT_Gln-NAD-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000036">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.110.10:FF:000003">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02090">
        <property type="entry name" value="NadE_glutamine_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014445">
        <property type="entry name" value="Gln-dep_NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090:SF9">
        <property type="entry name" value="GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006630">
        <property type="entry name" value="NADS_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8" id="KW-0520">NAD</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="9">
        <location>
            <begin position="6"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4371337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="709" mass="79672" checksum="D100257B8DC15A2A" modified="2023-11-08" version="1">MSRRLVTLATCTLSQWALDFEGNRDRILESIRIAKAKGATLRTGPELEITGYGCLDHFLEMDVYTHALEMLHSILSNEATHDILLDIGLPILFKGLRLNCRVLSLNGKIILIRPKMWLAQDGNYREMRYFTPWQTDEVDDYRLPELFHKLQGAETCPIGNAIVSTKDSSIGLETCQELFEPLAPHNLLTLSGAEIITNSSGSHHSLRKLDVRIGLIQQATKVCGGIYLYSNQQGCDGDRLYYDGSSMILCNGQILGQGTQFSLNDIEVIVSTVDLDEVRAYRSTPSRGLQAISAPKIKYIHVDFNLSSTGDDLDLDVMPTPPQPLSVHKAEEEIALSPACWLWSFLRRSKQAGFLLPLSGGLDSASTATLVYSMCRLVMSALDEGNEQVKADVKRIAGPHHEDGWMPKDARELCGSLLSTLYLGMAKQSSSETRSRAQRLAESIGSRHLNMDIDNVFLAQKELLTQATGFEPNFKVHGGTQAENLSLQNIQARSRMVTSYYFAQMLPTVFNRKGGGSLLVLASGNVDECLRGYLTKYDCSSADLNPIGSISKTDLKRFLHYAQTEFSLPILEEFLEATPTAELEPLTEDYVQSDEADMGMTYVELSVFGRLRKEQKLGPFGMFQRLVHEWRDKCTPRETAAKVKHFFHCYAINRHKMTTMTPALHMEDYSPDDNRYDLRPFLYPSFYESWSFKKIDEVVEKMEKRGAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9H4</accession>
    <name>A0A9W8Y9H4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004626</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9H4"/>
    <dbReference type="OrthoDB" id="2157530at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF73">
        <property type="entry name" value="HET DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G01020)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="3"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="610" mass="69359" checksum="E8F4D2264A5751C7" modified="2023-11-08" version="1">MAIQQYQEDNPVSMPRLWIDAICINQEQNTERSHQVSMMRDIYTGAESVIVWLGLAQTNEELAFLLTRYPTLLKVNEISTALVDLLNKSYWCRVWVVQEFVLAKAVEIWCGEFQADAADFDALWRADGLPLEFASIPQQIVNSNGWPLFKYRRDFRHSRRYKRELLGRRNSKTLKATFRLRDLLQAFETSQSSEVYDKVYGFLGIASTGRGERIRPDYSKSRVDLLVDVICNQCDDTIGRKDEDDYHFVIFLMRMLNVSRVEFARHVLQNSSEMQPFIYVFATTDFMVASMSLLGTIKEVGSSVHQAEAFQESMWTAGSLLRAPMHPGGLSNSDIRDMGLLVTFPETRLALEFEDYHIPNAVHQGKSEQLRQRLVAKSTETVMQSLIQRTRRKTGNDVENFQKPIQIDSKTLRQVFLNSFGAGAAVILSAMREQSERNTQPRYEQYSTFTGTNNIIGIICTSGSPDAPQITPGDHICVFSDDTVSKNALIIRRNEIEGQWIIIGFAVIITSISKSEPLGVVRSLQQAFGRGTLHTRTDWDIIDSVVGEMLHENLCFHCHLTDLLELSRCGILNETQLDRLLQQSLRDSDDDETHQCSLGTGKHDVLAFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA03</accession>
    <name>A0A9W8YA03_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004576</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA03"/>
    <dbReference type="OrthoDB" id="3760848at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="551"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="660" mass="73290" checksum="5FB911E597AFCF12" modified="2023-11-08" version="1">MVGSSGDTGTIPWPLLDTTISYEPINAHNSPESTTDTEHLTAQQALEDVIRNAEVRPEATERRTSRLRRRITNGFSRMRRTGTGETSFSSDGTSDSVPPVTTVSRAMNTETDPNLEGQDPSEEAVEAYMMKHARNGTKFQSVMKRIARRLPSPVEDHLDSSDNFTSLRTSTTPTTLRAAVRVLPEIKLLTDDFQEFSVAVDVEGVLYNRNLLPDTTIDVVFVVDNGYYVTKDCLNRALDAVNGALYHLGDGDRVALYTTHCTHHAVTGNKPDLHYPFKPFDAHSEQIFRDLTSGIAVCGTQVWEPPRPNPSMVEVILGIARSLDNAGLKAGRTHVILLSPAGHILHGVSKHFPELYVHQISPAALPCRPSLPLLDTVCTDICCQNVFISNWTSYQSVPGRIKHILKNARAEKPIGELTNVSIDIRTKHGCNIIEICGSKDIPILRLGQVHTMIVRIRVNRAETQGVDLDTNNPVLSSSLGVKGLRQGLKNSIASGAIKIHLFDVQAFHRNSLHASDCWNYIETPLLVIREMGGLAPPLDTSMEVYKRQYFQKFVQLEINEAKAEARNLLSELDGVNNQAKQYIERIAKEIDCHQAIQDYEQNYRQKLPLCPGPITIEASPHEWLMDLSNGKKTKRTGVAIVKREDMHGLIDGINGLERLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAG0</accession>
    <name>A0A9W8YAG0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_004322</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAG0"/>
    <dbReference type="OrthoDB" id="445341at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00431">
        <property type="entry name" value="cysteine_hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032854">
        <property type="entry name" value="ALKBH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31212">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31212:SF5">
        <property type="entry name" value="ISOCHORISMATASE FAMILY PROTEIN FAMILY (AFU_ORTHOLOGUE AFUA_3G14500)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="3">
        <location>
            <begin position="743"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="98"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="283"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="787"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="292"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="329"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="384"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="434"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="448"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="531"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="555"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="611"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="793"/>
            <end position="810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4371106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1156" mass="127124" checksum="1C2CD5FD3A65D2D9" modified="2023-11-08" version="1">MVSKMSSLMDLVSQNQPHFQTHQALLVIGLQNDFVLPDGRLPVNTKKGFLERIQALIPKFRELNGNVIWVQTLYEADRIATDANTGEGDALVVGGLIDGEESSTEGGDEEPTKDLLPPAQSRSSKHKQRALDLLKRVSARRKTIPQEVARASAEQDDELFLLKSEKKTPACIPNTHGAEFADVIARQFELPADLVIRTTNYSAFQGTSLLMTLRAKLVTEVYICGCITNVSVLATVIDAARHGVKINVVEDCLGYRKRARHDLALKRMEEFFDAYLVNSTDILSREPPVTPPQKQSSSPNGSRKGSKGSDKPLEAMVGKLSLSEDGRPASRSSNPRSPTKVLSGKQRTLSLVSVAESRKASQNTTTPIAEPSTKPATEPATKPGTKSATGQPTEPASETTDKEFADSLVKGAKVPGGKEQSPENEKNETNLVKTKIRMRSRPKKKKKEKDGETSKDKAESVPENGEPSKAVRHTPAEPPTTTGAVKPEPTEAQRTALMAKAGSVADLRERDARQQALKSVASVPVLAAKPSNDEKEKNRLSDYADRVRLSFSRAPKVEPNSDSKNEPPTPTKSTSVASTAEEGEAKEISANPSKPTTAIASLPAPAPSTPSAPTSAEKPAAMGKAPKLQSLATLPVLGPEDTIAEGDSRIIHDFFPADLRHPTDTSKPLKDLIFTQLYNEVRWQKMLHQQGEVPRLVCCQGAFGEDGSMPVYRHPTDQTLPLLHFSPKVHVIRKQAEKLIGHPLNHVLIQLYRSGNDFISEHSDKTLDIVRGSSIVNLSFGAQRTMRLRTKKSSSTTNSDVPSTTSTEDSSTQRETQRVALPHNSMFALGLATNAKWLHGIMPDKRLPAERSDAENAYNGMRISLTFRHIGTFLDAKESVIWGQGATAKEQRDAADVINGDEAESEKLIRAFSKENHSGTSFDWEKEYGAGSDVLHLHSTPAEDTGDLPILFASNNKIETTQVRICLAEAKIKYRLVEPPRLDNNSNKEFELDRQVSFRDTDTQHTEVTMASGILLYIDRFHPLSSSSSPSSSDSRYCTANAYPIMILTGGLLKAWLNRHVPTYLAEFENLLRCLEEEVERCGGPYVAGKRFSVGDAFVWPVVDEVRREWEEWGRGEGRYPVLGKYWEGLWKKKACVRGLGRGWRGQGGGWWGWGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAK9</accession>
    <name>A0A9W8YAK9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">LETM1 domain-containing protein ylh47</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">YLH47</name>
        <name evidence="11" type="ORF">N0V83_004162</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAK9"/>
    <dbReference type="OrthoDB" id="275278at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033122">
        <property type="entry name" value="LETM1-like_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044202">
        <property type="entry name" value="LETM1/MDM38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009">
        <property type="entry name" value="LEUCINE ZIPPER-EF-HAND CONTAINING TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009:SF1">
        <property type="entry name" value="MITOCHONDRIAL PROTON_CALCIUM EXCHANGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07766">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51758">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Letm1 RBD" evidence="10">
        <location>
            <begin position="247"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="49"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="475"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="492"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="537"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4372388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="565" mass="63368" checksum="E7137FD4902DB659" modified="2023-11-08" version="1">MYASRAASRAAPAVFRAGIRTSTRAHRPMLRTSPAIRILIPLRALQTETTSSNAAQNYPPPGFDPKQASKPLPKQDKQEPQQTQEKASPLLAKEETIIPKTGATSHPKTPAQDVQTMTELAAEKAAADTKEEKKIIAKKEEEKKKLTIYQKVKKELAHYWDGTKLLGFEIRISTKLALKMAAGYELTRRERRQLQRTVQDLGRLIPFLPFVIVPFAELLLPVALKLFPNMLPSTYEGQSAKDDKAKRLRSTRKEVSDFLRQTLKETGLPVSAENAQTAEFGEFFRKVRTTGEKPTPAEIIKVCKIFKDDLTLDNLSRPQLVSICRYMNITSFGTDNFLRYQVRVRMRQIKRDDRAIAYEGVDSLSVPELQTACASRGLRTYGVSPGRLRDDLQAWLDLRLKHGVPSTLLVLSNAFVYAQGKESEMTTQIDALEAVLSSIPEELYHEIELEVHNTEGAATNKQRLEVLKEQQELINEENEQTQAVENKATASPKDHEDIDEDDKPKRKEAEEAKEAKEAEESNEAEASTGDMTGGSAERAEQDDRARKGEEPAGKEEKSAEAAKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAW0</accession>
    <name>A0A9W8YAW0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_003875</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAW0"/>
    <dbReference type="OrthoDB" id="186625at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16180">
        <property type="entry name" value="EFh_PEF_Group_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051426">
        <property type="entry name" value="Peflin/Sorcin_CaBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212:SF3">
        <property type="entry name" value="GH27120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212">
        <property type="entry name" value="PEFLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="143"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="210"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="276"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="76"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="105"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4372102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="325" mass="36413" checksum="DD86927B11144633" modified="2023-11-08" version="1">MAYNKGFNPDRLPMHAEPEQAAQAMAGNQSSSGSRPPRISSARPEYNKPPPPVPSQRYDARPDERYGGGGGGGAYHDSRYDGRPSSDRYDSRHDNRYDDRHGVGSPPPATYGQGPPPQGYHGRPPIAQQSRPPPTPAPPRDANDREALWRLFGAVDKDNNGSLTEAELRTALVNGDWSPFDAHTVRMMIRMFDTDRSGSVNFDEFCGLWGFLSAWRGLFDRFDADHSGSISYAEFNEALIAFGYRLSQQFVTLLYRTYDRDGRNALSFDLFVQACISLKRMTDVFKKYDEDRDGYITLSFEEFLTGAQALFLFNSISAQTDTGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAX9</accession>
    <name>A0A9W8YAX9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ribonuclease T2</fullName>
            <ecNumber evidence="2">4.6.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_003905</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the RNase T2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.19" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAX9"/>
    <dbReference type="OrthoDB" id="435754at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033897">
        <property type="term" value="F:ribonuclease T2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.730.10:FF:000020">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.730.10">
        <property type="entry name" value="Ribonuclease T2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001568">
        <property type="entry name" value="RNase_T2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036430">
        <property type="entry name" value="RNase_T2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018188">
        <property type="entry name" value="RNase_T2_His_AS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033130">
        <property type="entry name" value="RNase_T2_His_AS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240:SF17">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00445">
        <property type="entry name" value="Ribonuclease_T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55895">
        <property type="entry name" value="Ribonuclease Rh-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00530">
        <property type="entry name" value="RNASE_T2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00531">
        <property type="entry name" value="RNASE_T2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040946780" description="ribonuclease T2" evidence="5">
        <location>
            <begin position="20"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="SnoaL-like" evidence="6">
        <location>
            <begin position="301"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4372132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="415" mass="46934" checksum="6CA1C94297B68A9E" modified="2023-11-08" version="1">MRAVLRNAAAALLAQTAAAQLYPNQSPLNHTCQLQAPLLSCPSQDPSKVDSCCVETFGGLVLSTQFWDTYTGLETQGQLLPRDTWSLHGLWPDFCNGSYTQYCDLNRQYDPIPSPNTTNGLPNGTVVAPYAGPNIGTFLEPFGRYDLLEYMNAYWIGWLQDNAGFWGHEFSKHATCFSTFNAPCYGPRYRQHEDVVDFFETAIKYYKRFPTFKWLEEECITPSNSTTYTYSKLRDVLFKNHGGVPFLGCSGPRYNTTTAGQGSTDNGYTVLTEVWYYEYFTLTSQVSDTENITSLLRQYGVVLATHSMSDLLSLYTEDGVLMAPGFQPAVGTKALKSSYERIFSTVKLEIDFSIDEIVVMNEDWAFARTTATGTKHWLKKGTKEDHHNQEMFVCQKTESEWKIARYCFSSMKPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB95</accession>
    <name>A0A9W8YB95_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003511</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB95"/>
    <dbReference type="OrthoDB" id="5339076at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018822">
        <property type="entry name" value="UPF0646"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10336">
        <property type="entry name" value="DUF2420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="412"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="539"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="706"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="421"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="539"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="568"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="715"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="787" mass="86218" checksum="8CB09DFC73ADBABD" modified="2023-11-08" version="1">MTADNMDLHSDDEIDFGDGDLDLDLEAAPSPRRQDEDVSIKDAASVGGNDVQAVPTEEDDFMADHEDLFEEGYRNEGEVGDGIVAADQSSTNNNALADQYDDVEEGYRNEGETGDGMVAAGQSSADKNVSADQYDDIEFDYYDDDGVLGEQKVESYTYEGQDAQDEAVPVNPDGVQDHDQAHQAADDEGVPVEGHADGQSDHHSDTHNEEGDHTDGGDGGVQLYGHEDLADEDEYEGSEHEVPHEDSDPATDAADNDDEDDQSFNLPPVTVNYTGNELWLFKEHDTDDSGDWLLEGLYLANKSMSELFHACRSSLGDDVSNEHEIGFRFDHLHGLELYEEHVASVAVKLHRLVGLYHTLQEQDGNNEPDSFYMTLLFRPRFMTFLSDIGKCAEQGIGYSGLEAAVATGETHFSNLNTGSSTEHESNEWENEEQREADYGDAEQTEGLLQEEVEHVEQESQAHTASDADYNQEVDDEHDSANTSNVPTTQQDSVGQLDAQISLAQSVNTTASDARREQEQDDLIDYSDDEGPEVTEQVTHASVNDQSVSSSTVQGDESTAVEEGANTTHDLDHNHHGIEHNDEGVLYAEDEHDNQFNDQLQEGIDDTTQSYQDYDDQAFGQDDLFQDFQANGDANQDYAGTEYNDLDQQQIDFLSGADLNAGDHDLTNANEFFDLTNASQPATDQGPASKIHEDDLIAYEDTTAQVDEEDGTADQSAAAVSSNANPTAASSTGLQDSSSRGQKRSVAEAGHGVEDALESIGMVVYLWRFKRTCNVDSLLLPDAKRPKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBZ7</accession>
    <name>A0A9W8YBZ7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Vesicle trafficking between the ER and Golgi</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SLY1_1</name>
        <name evidence="2" type="ORF">N0V83_004462</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBZ7"/>
    <dbReference type="OrthoDB" id="10251230at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="270" mass="29611" checksum="CE60EFF5EC2792BE" modified="2023-11-08" version="1">MALTLRDKQIASIKRILNLNAPLPDAGENDEGNNVPTNAATDSAAWKILVFDDMGRDVISSVLRVNDLRASGVTIFLNINSMRNPINDVPVVYFVEPTEPNIKKITADLAQGLYTPAYINFTNSISRELLENFGAMTVASSTAEHIAQIYDQSLSFVVTEPDLFSLNMKKSYYTLNSAKTSDAELDHVVDSIVGGLFSVCVTMKALPIIRCPKGGAAEDISAKLDRKLRDHLLNSRTNVFSESSAVGSRPLLILVDRNIDLLPMLSHSWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCI4</accession>
    <name>A0A9W8YCI4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_003543</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCI4"/>
    <dbReference type="OrthoDB" id="269227at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF123">
        <property type="entry name" value="GMC OXIDOREDUCTASE (AFU_ORTHOLOGUE AFUA_2G01770)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="3">
        <location>
            <begin position="259"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4373249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="563" mass="60267" checksum="34939A5E636B1BF3" modified="2023-11-08" version="1">MDSSFDYIIVGGGTAGVVIAARLSQYLPTCKIALVEAGPNAVDHPKVNDVSDPVAFLKLLQEGLVVDYSTTPQEHLDGRTIPNPGGRFLSGSSGANVGNWMRASAADHEVLAKRAGNERFRFERMVKYFKRVETHFDGAADGEWYGFDGPIHTTGGRKYPLREALRESAEKLGHRYDPDAGAKGYQAGLQDFVQCFRATSDSTATRQHSAKVYDLSGVEVFCDSPVARIIFDAARRATGIELTSGQKMHASKEVIVSCGTQKTPQLLMLSGIGPKDELSKHGISPVVVDAPAVGQNLFDHAIFTQYFKLKDPSKGLSFPFTGTLRPEYSQGLPVDFSLFANIPAADLTPYLKLDNSPNDDDALLFPKRNHIMTIPFYLPLFPSPALYPSISATAGTHISLGALNMLPSSRGTVTLSSRDPSAPPIINPRFLSTHTDRYIMRRAVRANLALTSTAPFADEIEGEVAPVDANPDAKRLSGLSTGSSDEEIDARLRAAVATVAHPMGTCALGSVLDGEFRVKGVEGVRVCDASVFPEPIGAMPSCTVYALAEMCAEVVSVGGAGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCM7</accession>
    <name>A0A9W8YCM7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Xaa-Pro aminopeptidase</fullName>
            <ecNumber evidence="5">3.4.11.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Aminoacylproline aminopeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Prolidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">N0V83_003618</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.</text>
            <dbReference type="EC" id="3.4.11.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the peptidase M24B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.9" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCM7"/>
    <dbReference type="OrthoDB" id="10261878at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01087">
        <property type="entry name" value="Prolidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007865">
        <property type="entry name" value="Aminopep_P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052433">
        <property type="entry name" value="X-Pro_dipept-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226:SF3">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE AN0832-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05195">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01011">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="8 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminopeptidase P N-terminal" evidence="16">
        <location>
            <begin position="33"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="495"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="571"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="689"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="713"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="750"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4373324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="854" mass="96061" checksum="031908C33003232B" modified="2023-11-08" version="1">MEVLDATSLAERLKWEAKEYWLHLEADSPLEKYPAKQHARRVQEKLGYDEGLIYLAGQPARNNEDSDMPAPFRQRRYFYYLSGIDPKRVIWNGRGSTRAEAIDKYDIDAVHYVDQLPGFVHDWASCYGNKHANLYVLHHLQVPPGLPDIRSRIDSVTLQPAMNIARMVKDDHEIDLIRKANDISSQAHREVLASILNFKNEAQVEGLFMDVCISQQAKQQAYDPIAASGPNAGTLHYDANNEDFGKRQLMCLDAGCEYKLYASDITRTFPLSASWPSKEAENIYKLVERMQEACIKGLAPGVRYLDLHILAHQIAIDGLLQLGILHNGTREEIFKVGTSRAFFPHGLGHHVGLEVHDVGQAELMSVRRGKPVYEQAPSLYPENYHLPVYDPKTCFGPTDPHSSHLEEGMVVTVEPGIYFSVYALQHFYLPSPIHSKYINAETVQRYLPVGGVRIEDDILITAKSYENLTKAPKGDAMLDIIRSGKPNTIMTPKLTRKASRRNHDDEESPLLRAPGIPSETPELMLKPLARASTMPPEFKQRGSVDFEPFDGPSLFSNFKRSMTAEEKIQRWRQSHGCTPIPNSPSTTSRKPIPVCGESIPHLKHVYMSTASHFGALSRGPQESESQRACKNCTILVQTLDRLRQNLNSSAQSSPTVEQNPMFVLPPQTLNSRQTRIDDLAARLQVVKDAANPASPTRKHGKPEMVRFREGNNPGANPTSQSQIQHIRHDPWTNTTPHNQARVPSHVNPMSSALPSRSLHSRRSLGILRATQLGEKGENLLPTMPANETQHRPESINPLHTSNTRGHIGDCIPFAPSVVNNDPLEHARQEIESLKLRLESLERPGRFETHRQERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCV5</accession>
    <name>A0A9W8YCV5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_002793</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-binding monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCV5"/>
    <dbReference type="OrthoDB" id="74360at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051209">
        <property type="entry name" value="FAD-bind_Monooxygenase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42877:SF2">
        <property type="entry name" value="FAD_NAD(P)-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42877">
        <property type="entry name" value="L-ORNITHINE N(5)-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4374054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="618" mass="69939" checksum="46AC2FF1824A6F50" modified="2023-11-08" version="1">METTSSGPTGPIDDTAPEIPQVKGASNGVKHPQVASTNGVNGTHKDAEFDPFELNKQYAFTPRKLKVITIGAGFSGLLMAHKFQHRFPEMQEMVDHTIFEAHSEVGGTWLVNDYPGVQCDVPAHIYAFPFDPNPEWTRFYASGAEILAYFKRVVKKWNLDRDLQLNTKVVAAVWKEELGQWKVTVEHAGVQRDEFCHILISGQGVLVHENWPDVPGLKDFKGHVTHSARWDHNYDYSNKRIAVIGNGSSGIQIVPQMARLPGTTVQNFIRGPAWVYYRAPPSKHLGRAVDDPNPAFTQEEIEKFRDPEYHHQYRKGIVSRTNKSFYIFLKGENNDAGMKLASEQMAEKLNHDKRLCDMLIPKWELGCRRITPGPGYLESFLRDNCDLTNSAITKITERGVETADGKTFECDVVICATGFDVSHRPRYPFIGQNNVNLREKWEDEPESYVSVMTPDFPNYFMMMGPNCLGGHGSLVESLNWTGDYFVKWIKKLSTEDIKYVAPKRSVVDAFVKYGDEIHKTLVWTGGCKSWYKRGRVDGRVTALFGGSAHTFNRLLSEIRGEDFDITYNTANPFRFMGNGFTKYEMNESSDLSWYVEMAEEAQGRGPARQKAMNGTVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCY9</accession>
    <name>A0A9W8YCY9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA-binding RNA processing protein rpp1</fullName>
            <ecNumber evidence="5">3.1.26.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RPP1</name>
        <name evidence="5" type="ORF">N0V83_004045</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCY9"/>
    <dbReference type="OrthoDB" id="17948at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000053">
        <property type="entry name" value="Ribonuclease P complex subunit Pop2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002738">
        <property type="entry name" value="RNase_P_p30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01876">
        <property type="entry name" value="RNase_P_p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="242"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="242"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="287"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4372271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="303" mass="33916" checksum="6AD80C86E2BEE6EA" modified="2023-11-08" version="1">MFHDLNVPWTEATRELQRTVAFLDELGYDVVALTHTYSGKLPADLTSPIPNPLPFPTPARLRILRRCNIFLTDSASNFRIPQLQQQYDLVAARPTDERTLQQACQSLDVDIISLDLTRKFEKHFKFPMLGTAIARGIKIELCYSQGILSNDPMAKRNLIMNATQLIRVTRGRGLIFSSEAKTVLGIRAPSDVINLASVWGLGTEKGKDGLTREPRSIVEFARLKRQSFKGIVDIVYGGEKPVEKSAAEAKDKQSQKGKGTRNQNGKRPGDSLESMPLRQAEDDKPISKRQQKRNKMAKTDVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-06-18" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDF6</accession>
    <name>A0A9W8YDF6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation factor G, mitochondrial</fullName>
            <shortName evidence="10">EF-Gmt</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Elongation factor G 1, mitochondrial</fullName>
            <shortName evidence="10">mEF-G 1</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Elongation factor G1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">MEF1</name>
        <name evidence="13" type="ORF">N0V83_002800</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="198619at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070125">
        <property type="term" value="P:mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01886">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01434">
        <property type="entry name" value="EFG_mtEFG1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04097">
        <property type="entry name" value="mtEFG1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04091">
        <property type="entry name" value="mtEFG1_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000003">
        <property type="entry name" value="Elongation factor G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000001">
        <property type="entry name" value="Elongation factor G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000022">
        <property type="entry name" value="Elongation factor G, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000015">
        <property type="entry name" value="Elongation factor G, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000558">
        <property type="entry name" value="Elongation factor G, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_B">
        <property type="entry name" value="EF_G_EF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047872">
        <property type="entry name" value="EFG_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004540">
        <property type="entry name" value="Transl_elong_EFG/EF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00484">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009381">
        <property type="entry name" value="PRK12740.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43636">
        <property type="entry name" value="ELONGATION FACTOR G, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43636:SF2">
        <property type="entry name" value="ELONGATION FACTOR G, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 13" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tr-type G" evidence="12">
        <location>
            <begin position="869"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="96"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="66"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="155"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="355"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="381"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="426"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="497"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="578"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="675"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="691"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="878"/>
            <end position="885"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="954"/>
            <end position="958"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="1008"/>
            <end position="1011"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4374061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1570" mass="169295" checksum="D3264C5BC3B58CD2" modified="2023-11-08" version="1">MSSGVRNLRAMFENQGAASSPEPRGRSPIGDASTECSSPSTKVRASFVPVEPSIPIATDLGATKGTPINSVSAQRRESFSVSQDNIEELAELKKAVSQEKAERKKSDAIPEAVPEQAVLSREASISPPPIRNPPDGDMPNLGAIMKGSDFPEPAKQVEKPRVEETPAKPRETPVKKAEKPKSTPAPTPQPKQASVKQTDKPTPASAAQSKRDTVKKAEKPTPAPATPSKPVKEVETPIPAPAIPAKQDTTDKVEEPSPAPAPATEVDQNPVKEEEKPTPAPTIQSLEIPAEEEKQPTPAPATQAEEVPAKVEQKPAAAPVVQPQETVAEEAKELMPAPVAEKPAEDTDKAVTAVSEVSPSNTTEGPAEGAVSSEAAVPHPSATTQASSTTSTNGTPKVAESKAKTNGTPAAKKPEPKKPAAISTVKASLAKPSSAKSPLPKTAPRTPTTPKHTAPKPAVAAPAAKTSPAAKVHPKPAATKEPTKPPAAKTSRTSLRPAATSSIASGPTASAAAKTKTAAPENKKPATTKPVTAAPHASAFKKPRPKSPTRPVKLPSHLTAPTAASAAKHGEEAKLTRKPSTATRPKAAPAAKPSRASIAPKRPDSRASTVGAPKEGFLERMMRPTAASASKTHEKPASPPRKAPAKAPVSSALHKGKKKVEEVASKTKAAVTNGQHEEEHQDGHHADDTSAGASNPTDQVDASATADGATEETTKAATPTQEVDSSAAELQTPHFQGEAIHEGSAFPTSTIARREQSSHCCPIDCSDPFTMRTVDHCGVAHTIHQWHKDRHVDSQPVKASAKKMAICCCTSVSVHQRFSRSWSHRCGDLEREWKLIKRRRLEQAKENPSALTQEKIVENLDPVEWERLSRVRNIGIAAHIDSGKTTATERVLFYTGRINSIHEVRGKDAVGAKMDSMDLEREKGITIQSAATFCDWVKKNDKGEEEKYHINLIDTPGHIDFTIEVERALRVLDGAVMILCAVSGVQSQTITVDRQMRRYNIPRISFVNKMDRMGANPWKAVDQINQKLRIPAAALQVPIGREDGFLGVVDLIRMKAIYNEGAKGEIIRETDEIPSDLVELCQEKRQKLIETLADVDDEIAELFLDEQEPSVQQIKAAIRRATIALKFTPVMMGSALADKSVQPMLDAVCDYLPNPSEVENIALDKKRKEAPVKLVSYNSLPFVGLAFKLEESSFGQLTYIRVYQGTLKKGMNVFNARSDKKVRIPKIVRMHSNDMEEVPEIGAGEICAVFGVDCASGDTFTDGGLGYTMTSMFVPEAVISLSIKPKHTKDTPNFSKAMNRFTREDPTFRVHVDPESQETVISGMGELHLDIYVERMRREYRVECETGQPQVAYRETITKRVNFDHTLKKQSGGSGDYARVVGWMEPSEALAENKFEQQISGGTISEKFLFACEKGFMASTAKGPLLGHKVLGTSMIINDGATHAVDSSEMAFKNATQQAFRKAFQAGAPQVLEPLMKTTITAPNEFQGSVVGLLNKRNAVINDTEIGPEDFTVYADCSLNSMFGFSSQLRASTQGKGEFSMEFSHYSPAPPQLQRELIAKYEKEQKARND</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDM8</accession>
    <name>A0A9W8YDM8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003214</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDM8"/>
    <dbReference type="OrthoDB" id="3359339at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="121" mass="12616" checksum="126A46853770630C" modified="2023-11-08" version="1">MSSEHKNTNPPKVAEQAERDLGTEAARKGHNTSDSTLESGVDEAVTTKFPGSTVEYGSGASGSGNNRDIPLSEGGDLNPKTGKPYKAGDFARGGVGAPEARDEKYAQTHGGNDDVRGNIRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE30</accession>
    <name>A0A9W8YE30_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Succinyl-CoA:3-ketoacid-coenzyme A transferase</fullName>
            <ecNumber evidence="3">2.8.3.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_003454</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 3-oxo acid + succinyl-CoA = a 3-oxoacyl-CoA + succinate</text>
            <dbReference type="Rhea" id="RHEA:24564"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:35973"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="2.8.3.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Ketone metabolism; succinyl-CoA degradation; acetoacetyl-CoA from succinyl-CoA: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the 3-oxoacid CoA-transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.3.5" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE30"/>
    <dbReference type="OrthoDB" id="1933379at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008260">
        <property type="term" value="F:succinyl-CoA:3-oxo-acid CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046952">
        <property type="term" value="P:ketone body catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1080.10:FF:000001">
        <property type="entry name" value="Succinyl-coa:3-ketoacid-coenzyme a transferase subunit b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.10">
        <property type="entry name" value="Glutaconate Coenzyme A-transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012791">
        <property type="entry name" value="3-oxoacid_CoA-transf_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014388">
        <property type="entry name" value="3-oxoacid_CoA-transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004165">
        <property type="entry name" value="CoA_trans_fam_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004164">
        <property type="entry name" value="CoA_transf_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02428">
        <property type="entry name" value="pcaJ_scoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707:SF60">
        <property type="entry name" value="ACETATE COA-TRANSFERASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707">
        <property type="entry name" value="KETOACID-COENZYME A TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01144">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000858">
        <property type="entry name" value="SCOT-t"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00882">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01274">
        <property type="entry name" value="COA_TRANSF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="active site" description="5-glutamyl coenzyme A thioester intermediate" evidence="4">
        <location>
            <position position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000858-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4373163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="543" mass="58005" checksum="F27111A3A97557DF" modified="2023-11-08" version="1">MDALPSSYRLLAALPSRICSRRPLPSQLRAARSLAPQWRFTNHRRNISMVADRAPTIDRSKSKLFKDADEAVADIQSGSTVLSAGFGLCGVADTLIQAIQKRGPQSLHSLTAVSNNAGIEDVGGLALLTKNGQVEKLIISFLGNNKALEKQYLGGGIEIELCPQGTLAERIRAGGAGIPAFYTPTAVNTLLQDGEIPAKFDKEGKAIGYGQKREVREFNGKKFLMETALTGDVAIIRAHKADEAGNCVFRYTTKAFGPIMAKAARLTIVEAEEIVPIGTFDANDIDLPGIFVDRIVPSTAPKNIEIKKLRSPSTSSKDKTQSKTDHRARIARRAAKELKHGYYVNLGVGIPTAAASFVPDGVKVWLQSENGILGMGPYPTEDTIDPDIINAGKETVELLPGASTFDSAESFGMIRGGHVDVSILGALQVSASGDLANYMVPGKVFKGMGGAMDLVSNPEATKVVVATEHVAKDGSSKIVQECNLPLTGAKCVSTIITDLCVFEVNRKRGTLTLTETAPGVSVEDIKAKTDAKFEVSPDLKEME</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEQ1</accession>
    <name>A0A9W8YEQ1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Epoxide hydrolase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_003549</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEQ1"/>
    <dbReference type="OrthoDB" id="7130006at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097176">
        <property type="term" value="P:epoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010497">
        <property type="entry name" value="Epoxide_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016292">
        <property type="entry name" value="Epoxide_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661">
        <property type="entry name" value="EPOXIDE HYDROLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661:SF39">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G08960)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06441">
        <property type="entry name" value="EHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001112">
        <property type="entry name" value="Epoxide_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epoxide hydrolase N-terminal" evidence="4">
        <location>
            <begin position="18"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001112-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4373255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="397" mass="44607" checksum="BD16CF141901B3F7" modified="2023-11-08" version="1">MTSSNFSTVPKGAQQQPTPFELKIDESKLQELQTLLRLSPVAKETYENLQDDGSHGRLGVSRKWVADTKKYWEQEYDWRKEEAHINSFLNFKTKITDDDGGVFDVHFVALFSSNPSAIPVAFFHGWPGSFLEFLPLMGILSKKYTPETLPYHIIVPSLPGFTLSSDPPLNRDWKVADTSRIMHKLLLSLGFGSTGYLVQGGDIGSLVSRVIAATYAECKGMHVNFMFIKDIATKSSPDDEISEAEKKGLDRLNQFMSVGHAYAQEHGTKPSTIGLVLASSPVAQLAWIGEKFLAWADPSTTPSLDTILSDITLYWLTGCYPTSIYTYREAKELLYVDKPIGYSYFPYELAPVPKAWAEKTGKMVFFKSHDKGGHFAALERPEQLWADFEEYVKAAWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YES6</accession>
    <name>A0A9W8YES6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003594</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YES6"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="158" mass="17159" checksum="287451B654EB2A33" modified="2023-11-08" version="1">MESNSTQQTNNLNFDASSVAEAIRGMGGESALARVARRQGLQVQSLPPPILTPVARRALRELYAITQDILGGVPNDELQEAARRFCNDLTPELARSKKEWDANGKRSGEKRDGAALGGGENGEGDTEEDETDEEGNDENEDVDNEGKELTLTIRTRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEZ3</accession>
    <name>A0A9W8YEZ3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="6">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ARO8_1</name>
        <name evidence="7" type="ORF">N0V83_002714</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 9">
            <text>D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="5">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="5">
            <name>Cd(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48775"/>
        </cofactor>
        <text evidence="5">Binds 1 divalent cation per subunit. The enzyme is active with manganese, cobalt or cadmium ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the class-II DAHP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEZ3"/>
    <dbReference type="OrthoDB" id="2338at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002480">
        <property type="entry name" value="DAHP_synth_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21337:SF0">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21337">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE 1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01474">
        <property type="entry name" value="DAHP_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0104">Cadmium</keyword>
    <keyword evidence="5" id="KW-0170">Cobalt</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602480-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4373975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="465" mass="51402" checksum="13E355171957DE98" modified="2023-11-08" version="1">MEPTNGQHEEWSPASWRSKPIKQVVTYEDQSAVNSALTKLTSLPPIVTPTEIARLKSSLRDAALGKAFLLQGGDCAELFSYCADNPIDAKIKLLLQMSLVLIWGSNKPVIRIGRIAGQYAKPRSSPTEVLDGKEIPSFRGDILNGYDPEHRRVDPERLVSAYFHSATTLNYIRGQLASGIADLHNPLDWELGHVRDQELQSKYQKIVSSISDSLRFMKTIGADTSGQLQTVDLFTSHEGLVLEYEQSLTRRLKNPAGYKPSRASSDGKGWYNTSAHFIWIGDRTRQIDGGHVEYFRGIENPIGIKVGPSMKNDELVELLDIVNPNKEVGKITLITRYGAEKVEAMLGPHIEAVKSSGHIVVWQCDPMHGNTRSTPTGIKTRSFNSIFSELSSALKIHKQHGSFLGGMHLELTGDAVTECTGGSEGLVDEDLSLNYTTYCDPRLNEKQALELAFLVAGHYRADGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFH2</accession>
    <name>A0A9W8YFH2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001573</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFH2"/>
    <dbReference type="OrthoDB" id="4150467at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="588"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="190"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="307"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="422"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="307"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="422"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="435"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="451"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="481"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="494"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="516"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="531"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="544"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="597"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4376290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="680" mass="74404" checksum="5CF2095688F3589B" modified="2023-11-08" version="1">MQVSNLLAEPQVSGVRRFPETVEELIAGRQYDSRQSASGYDMLPQREDSGMMDIYPSTETASAHDSLSPTKQVQPKHISFELLLPQSPAHKARLPMRVNIYPHDTTDSIITTVKNFYGLYERRGVIFEDRHGNTLIARFENFEHGMVVYVRVSPEDPDADDYSPAPRQPTASPRRPRPQLEEAFQMLPPSINHHAGSRAGSRTGRQSHSPVPGHGRRSVSASKRIRPSMKSRGNSSHGSFADANGDAYSDSEGDHGSVTSSRRSRKEPLASADISVDNIVEGGRRKRAKFDSSELPLFVPPQVPMTASLSSVSPQRRISGNTAGSPYSVNQQTFSYSHPLPSPQSYGQGESSYMQGLVTPYSASSGPVQGYRARTRGSAQHAHYRYSGSGGVLPTPETTLGSISVISDEDVARQLMRLGDASNFSTHGRTSTSTLDDALSGKADAASSSGESDEGSEDERELPPLPYNMGRMIPMPRVYDETESSGEDYDDNRDDSFKGESDEIMPDEHRDQRMQSGVVKARSSVSSKSGKPAKPRALSKNKTKVNASSKPPMSPTSLPSQSRKASSASLNFQHQLGVDEEDLSSKPRCQRCRKSKKGCDRQRPCQRCKDAGIGADGCVSEDEGNGRKGRYGRHMGVSVKKPSVATSMAPPPMHDYNTPMDTMHTAFSSSNMDKNKKRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFJ4</accession>
    <name>A0A9W8YFJ4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_001601</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFJ4"/>
    <dbReference type="OrthoDB" id="433512at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="415"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="485"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="102"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="43"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="367"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="384"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4376318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="525" mass="58632" checksum="04EAFA7BC2D15EF1" modified="2023-11-08" version="1">MAALTLKNNWSYLRSEESVNTLNVFDNRYWDWDWEPERSIRRSCSSASTTTRHQSSNKPRPGPTAVDASPPNNSSPLRIKEKTQRLQYITGQLDDNGFDAGVFLVAASGFLTDSYLLFVTNTVIPSIIYVYMHDRPEEARDFEMWINLTTLIGSIVGQVLFGILADLLGRTSLYGWELIIVIISTLGFAFTSEGVLPGAQTKLGPSLDLKTSFYCWRFVQGVGIGAEYPLSAVLTAEWASVDYRAQMVAAVFTMQPLGQLCAAIAGYSAIAIVNRSFRLSDYGVHGTADSRALVDRVWRGIVLFGALPSVLALIFRFYLTDPGRYTLEVQEDIDQAVEDTDRQVSRQLLSRLKKVLPWKTKTLTEPEDIELPRAQPRHRGTGHTADTSSSDPPQTHDHRQSLGDLYTYLWTERNIILLFGTCATWFLLDIVFFGLGISSPDTLAVVWADQPVPSVSNTTVPPWNPDPTHPDYTIVDVLKSNSKELILASALGALGGSILLTITINQFKRKWLLQWTKYTHLLIPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFJ6</accession>
    <name>A0A9W8YFJ6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001423</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFJ6"/>
    <dbReference type="OrthoDB" id="2141239at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040823210" evidence="2">
        <location>
            <begin position="18"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="176"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4376142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="382" mass="37210" checksum="524745627AA19CE3" modified="2023-11-08" version="1">MVSKVFVIAAAVAYVEARFGQEQVPISAISAVQGGAPGAAATVAGAAISDLLGGANSCAKLTRADQVFSELGGGADALAAAIGLVTAEKNTNPFANDNVQNVCGDASLPATPELRGITPLIDPAVDVDGKVAALSKSSAASPLAADGLSVFDLLDQAGLGSLVVSQAAAGGAAAGNANQQASSSNNNTQQAASSTQAAASQQTAAADDNADACDDSNANADAAASNNDQQAAAPAAAANNTNVAAGVQASSIAGLDFGKCTPTITFQGGEGNRPATEFTFQIADTVARGGQGEALNPNIITNALCNQLTNVCDANDAAKAACLDAKAQVAAAGTRDKSTADLFNTALGFAGAVTNPAGGPADPPAAAAQSGRKMRRGLRAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFK3</accession>
    <name>A0A9W8YFK3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin-specific chaperone A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RBL2</name>
        <name evidence="5" type="ORF">N0V83_002047</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the TBCA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFK3"/>
    <dbReference type="OrthoDB" id="296187at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048487">
        <property type="term" value="F:beta-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004226">
        <property type="entry name" value="TBCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036126">
        <property type="entry name" value="TBCA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21500">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21500:SF0">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02970">
        <property type="entry name" value="TBCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46988">
        <property type="entry name" value="Tubulin chaperone cofactor A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="81"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4374968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="116" mass="12856" checksum="B3D7FEEC270F5F7A" modified="2023-11-08" version="1">MAPPSKLAIATGVVLRLVKEEASYHKEIEQQEARIKKSETSEGDENAEYTLRQERQALQETKNVLPGMKTKIEQALERLEEELESNKEAGGAASTEDVTKAKEAVAKAREAMREAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFW2</accession>
    <name>A0A9W8YFW2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Brl1/Brr6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001767</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFW2"/>
    <dbReference type="OrthoDB" id="5961at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040202">
        <property type="entry name" value="Brl1/Brr6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018767">
        <property type="entry name" value="Brl1/Brr6_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136:SF1">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10104">
        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01042">
        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Brl1/Brr6" evidence="3">
        <location>
            <begin position="275"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="481"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4376483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="502" mass="55962" checksum="997FCEB9F74D3FDF" modified="2023-11-08" version="1">MSRGHRASTTPMEFEWTNQTGPVDSQSPFLTAGQQSAKKRKLSTFASAATLEQGRPLTSWPPFGQPGPHSVLDSPSKNAFATPNRLRDPDNRPFFFSQSSSKPLPSIPAHVQSAWEPRTPASNYDSSGGETPNTPQVDSDAGTPDTQTAMSMGRLGLGNGESNSPKKAGRRDSWFKRLTGISSPTPTKEREKDVSQKYYSKKAENRIVKRRSERTERSRSKKRHAMRDDGDDSDDAQPGAQGAAKEQGVVKQTFAMSIAGFLSWVEAHPNLPSVLSFYLQLSVNFFLGSAFLYLIYSFWSGVMTDVEIESSKHASEVMVEIAACAVEYNRNRCRPDEVVPAMEKACGVWETCMNRDPRKVARASVTAKTFAMIFNSFVEEFSYKSMIFTAIIIFGGFNLSNWAFGLLRSQQQHQHQTPNPNDFVPQTPHRVPSNNYIDYNQQAYQQNWQQNTPYQTPYGGMRHIEAPPLVHAQSMPAVPTASQEGVALADERKTPRKKGLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG51</accession>
    <name>A0A9W8YG51_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001973</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG51"/>
    <dbReference type="OrthoDB" id="5086500at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046536">
        <property type="entry name" value="DUF6601"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34414">
        <property type="entry name" value="HET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34414:SF1">
        <property type="entry name" value="SUBTILISIN-LIKE SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20246">
        <property type="entry name" value="DUF6601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="225" mass="24989" checksum="2752DA0232F77E5B" modified="2023-11-08" version="1">MVIVIIDLDLRKASPAFPTLPGLLENAPLGFLLSYVALVEREIDLNMAINHGLMPKEITWPSWLTLVEEIVCVSSQAKSYLVDTSPASSEVSLKHRVPVNPRFYYGELRLGRLNWIYRLVLGRPRGYMSGCTTYGAFMRENINSLITLFAYTTIVLSAMQVGLGTEFLANNYAFGMASYVFSIFSILAPLACIVAILGVIAMMFVINLTRTLKIRGTRRRQGAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG55</accession>
    <name>A0A9W8YG55_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA-splicing endonuclease subunit Sen2</fullName>
            <ecNumber evidence="4">4.6.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SEN2</name>
        <name evidence="8" type="ORF">N0V83_000164</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the tRNA-intron endonuclease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.16" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG55"/>
    <dbReference type="OrthoDB" id="10249562at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000214">
        <property type="term" value="C:tRNA-intron endonuclease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000379">
        <property type="term" value="P:tRNA-type intron splice site recognition and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22363">
        <property type="entry name" value="tRNA-intron_lyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1350.10:FF:000007">
        <property type="entry name" value="tRNA-splicing endonuclease subunit Sen2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006676">
        <property type="entry name" value="tRNA_splic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016589">
        <property type="entry name" value="tRNA_splic_SEN2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227:SF0">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011789">
        <property type="entry name" value="tRNA_splic_SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA intron endonuclease catalytic" evidence="7">
        <location>
            <begin position="346"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="137"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="152"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="376"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="384"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR011789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR011789-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="482" mass="54733" checksum="0BF10B18C56436D0" modified="2023-11-08" version="1">MTEATPVQASPQGAPRENGVASENPEKAANGAPRKPRQKRPNYHEIHAKPLPLEVYPLPVFIPHNPISIVRIAIGLLSHSLWPPKSHTKVHRAYFSPETQSVHVTDPASIRALWEQGFWGKGSLSRSEPQWLVAEKRRRGAAASKTSAEVTQSRREERKQFKLERAKAQREAIEQQLRQEGKLDADGSIAELVENGDMAESPRGTIYTPNAGDVVTLTGDSIVSEVDKSLTSHALSWGLDAVDVDAIEEAEVQDLEHLQLSPEEAFFLTYVLGVLDIVTDGDLAVAAALKPYPAWYLLRLYCAYASSPTQQEMIENLHQAYQSCEDHQQVHHTLPSTPYIEPDNGFLLKYVVYHHFRSLGWVVRPGIKFAVDYLLYIRGPAFTHAEFAIMIVPSYSDPWWSQNPDKLREKEKEYKDWWWFHRVNRVQTQVMKTLLLVYVEVPPPWDASPSGDNFKLDVGAVLKKYSVREFVFKRWSPSRNRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGL7</accession>
    <name>A0A9W8YGL7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="8">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ARO4</name>
        <name evidence="10" type="ORF">N0V83_001743</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the class-I DAHP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGL7"/>
    <dbReference type="OrthoDB" id="4699125at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000005">
        <property type="entry name" value="Phospho-2-dehydro-3-deoxyheptonate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006219">
        <property type="entry name" value="DAHP_synth_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00034">
        <property type="entry name" value="aroFGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009395">
        <property type="entry name" value="PRK12755.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE DAHP SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225:SF12">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE, TYROSINE-INHIBITED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001361">
        <property type="entry name" value="DAHP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP synthetase I/KDSA" evidence="9">
        <location>
            <begin position="57"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR001361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4376459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="375" mass="40172" checksum="FA6F155F392695F2" modified="2023-11-08" version="1">MASAQANLDMPAFRDAPSDDVRVLGYDPLIPPALLAAEIPIPPHAEKAVVQGRKDAAKIITQKDDRLLVMVGPCSLHDPELAIEYCQRLKTLADKLQDDLCIIMRAYLEKPRTTVGWKGLINDPDIDESYQINKGLRVSRKLFVDLTSSGMPIASEMLDTISPQFLADLISLGAIGARTTESQLHRELASGLSFPIGFKNGTDGGLTVAVDAIGAAAAKHHFMGVTKQGLAAITRTAGNEHCFVILRGGSKGTNFDKDSIKAVREALGKKGLPEVMMVDCSHGNSNKNHRNQPLVAKDIAEQIRNGETGIVGVMIESNINEGNQKVPAEGPSGLKKGVSITDACIDWDSTVTVLEDLASAVAERRANKAKTNGVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGN0</accession>
    <name>A0A9W8YGN0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_002264</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGN0"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13738">
        <property type="entry name" value="Pyr_redox_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4375180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="524" mass="58941" checksum="F0D4F7EE0C0740AB" modified="2023-11-08" version="1">MGRIGSIVQYFFTHPILFLYQILQYVLDKVLSPTPPPPHAHLVRPKIAVIGAGLTGVSAASHIVGHGFDCRIFEAGPKENVGGIWSKVNNTSGLQIHSIMYRFHPSVHWKKGYPDRQQIVSQIRDLWERYGLEEKTEFNTRVERVYKDPHGRWIINDPSHGRFDGVVAAIGTCGDPKMPTLPGQENFKGEIWHSSQLDGKSAKGKKVAIIGGGASAVEALEFVARSEASHTNVLARSEKWIIPRNPLVDALLAMNVLGSETLFSWIPEGILRLFFYRDLYDISPPSHGGKGLFEETPMVNDHVLDLMRNGTASWLRGDILGYDDSGKGIKFNKRSRGVPKNGPGSEKLIEADIVIMATGYQRPSLGFLPDEVFQEPYEPPNWYLQVFPPEHPSICANNCTYVNAIGTVGNYHIGIYTRFLLMYLVDPLARPNPWWMKRWIDMTRFVKRAAPGGAFDFFTYSELIYWFLFTIVINPFRWKWAAFVLCGIGKGLPLSVIQQENKARNGLGMRNALTNGGEQAEKEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGQ5</accession>
    <name>A0A9W8YGQ5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Flavin-containing monooxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_000434</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FMO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGQ5"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4377607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="486" mass="53119" checksum="4788F77D388DC908" modified="2023-11-08" version="1">MSSIKKNVAVIGAGISGVTAAAHLRKEGLDATVFERSSAAGGIWLYDSRKPLEPAYPSVLASKAGSSYTKGVGEDLQLEKLLHAPPGPCYIGLKNNVGTRLLETTLNQFPAGTEDFVTHDVLKDYIQDTAAITGVHEITQYETEVKHVSRKGGNWIVETATLHSDEAGRIMQKTSHSEFDAVVVASGHYHAAKVPKTPGLADWKRTWPDRVNHSKGYRTPKEYQGKNFLLIGGSVSATDIARELGPIANNIYQSHRNGNFDIPPTLLPENATRVGEVVSYDAPINGWSKEPLEASDAIPATVTLECGEKLCDIHHVILCTGYHITLPFLPHLHSDATLVSAAGDELLVTDGSQFHNLHKDIFYINDPTLTFVGVPFFTATFTLFEFQAMVVAKVLSGQAKLPGEEAMRAEYDERVRAKGFGKGFHSLKDRESEYVDELLACVNKDLEKLGVKKLDGHSEKWHAAKAEQVIRMRGLFGGTPGPEKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGV7</accession>
    <name>A0A9W8YGV7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGV7"/>
    <dbReference type="OrthoDB" id="2151417at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38849">
        <property type="entry name" value="SMALL SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38849:SF1">
        <property type="entry name" value="SMALL SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040930895" description="Small secreted protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="17257" checksum="C60E09797CDF9E8E" modified="2023-11-08" version="1">MRFSTQIFALVLSLSATSLGAPTTKRALTAQSFNEFTVSGGVAGNALAEVNANFPVDTTDLAGVSASDLAIISSAAQISEQAEVATGGFNDALKAVSGDAATALQNGKIKNKVLKLQTDVLRLQIEQAQGKSGLDSQLAAQQKKLATNVALDVKAKGQASTAISFDGTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH74</accession>
    <name>A0A9W8YH74_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Argininosuccinate synthase</fullName>
            <ecNumber evidence="3">6.3.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Citrulline--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ARG1</name>
        <name evidence="14" type="ORF">N0V83_000784</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the argininosuccinate synthase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH74"/>
    <dbReference type="OrthoDB" id="1688907at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004055">
        <property type="term" value="F:argininosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000053">
        <property type="term" value="P:argininosuccinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000050">
        <property type="term" value="P:urea cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01999">
        <property type="entry name" value="ASS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000019">
        <property type="entry name" value="Argininosuccinate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1260.10:FF:000003">
        <property type="entry name" value="Argininosuccinate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1260.10">
        <property type="entry name" value="Argininosuccinate synthetase, chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00005">
        <property type="entry name" value="Arg_succ_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048268">
        <property type="entry name" value="Arginosuc_syn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048267">
        <property type="entry name" value="Arginosuc_syn_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001518">
        <property type="entry name" value="Arginosuc_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018223">
        <property type="entry name" value="Arginosuc_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023434">
        <property type="entry name" value="Arginosuc_synth_type_1_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024074">
        <property type="entry name" value="AS_cat/multimer_dom_body"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00032">
        <property type="entry name" value="argG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001770">
        <property type="entry name" value="PRK00509.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587:SF2">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20979">
        <property type="entry name" value="Arginosuc_syn_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00764">
        <property type="entry name" value="Arginosuc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69864">
        <property type="entry name" value="Argininosuccinate synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00564">
        <property type="entry name" value="ARGININOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00565">
        <property type="entry name" value="ARGININOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginosuccinate synthase-like N-terminal" evidence="12">
        <location>
            <begin position="5"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Arginosuccinate synthase C-terminal" evidence="13">
        <location>
            <begin position="178"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4377954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="415" mass="46952" checksum="077882043479E9AE" modified="2023-11-08" version="1">MAKGRVCLAYSGGLDTSTILRWLIEEGYEVVCFLANVGQEEPWDEVEKKALKIGALKMVILDLQKEFVEELCFRAVQCNASYEGRYLLGTSLARPVIARAQIRVAQQEGCQFVSHGCTGKGNDQVRFELAFYTLQPTIKVIAPWRLPEFCDRFKGRQDLLDYAEKHGIPVTSTKAKPWSMDANLAHCSYEAGILEDPDQSPPDDMWTMTQSPLSAPDQPTDITVYFEKGIPIKVVTPDKTYTDSVELFTALNKLGFTHGIGRIDIVENRFIGLKSRGCYDSPAMTILRLAHLDLEGLVMDAQVRNLRDQFVSHNWSYQLYNGMYFSPEREFIENSLLFSQRRVNGEVRMKLYKGNAYVLGRSSQTEKLYSEEEASMDTLDNFSPMDTTGFIAIQSIRLKKYGLQKAEEGANMSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH79</accession>
    <name>A0A9W8YH79_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_000870</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH79"/>
    <dbReference type="OrthoDB" id="48317at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08249">
        <property type="entry name" value="enoyl_reductase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047122">
        <property type="entry name" value="Trans-enoyl_RdTase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348">
        <property type="entry name" value="HYPOTHETICAL OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348:SF2">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C2E1P3.01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="4">
        <location>
            <begin position="13"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4378040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="342" mass="36543" checksum="BFD6D04E2FAA5957" modified="2023-11-08" version="1">MSVQRAVVHKSEGVTELRNDVPLPTLPEGNWALVKTKAVALNPTDWKNIARAVSPGAIAGCDYAGVVEEVGKGVTDLKVGDRIAGFARGGDPADHSNGAFAEHIKGKVGIHLKIPDNTSFEDAATLGVGITTVGQGLYQELGLPLPPNKVHEPTPILIYGASTATGTLAVQFAKLSGYEVIATASPHNFDLLRKLGADQVFDYKDPEVGDKIRKATNDKLKLVFDCIAEGNAFQISAAAISSSGGHLSALLPVKDFPRDDVKTNFTFAYTALGEKYNDRFGASQTDYEFGAKFWKLSEDLLKSGKLKTHPTEVRKGLEGVPQGLKDLEDGKVSGVKLVFKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHK9</accession>
    <name>A0A9W8YHK9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001662</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHK9"/>
    <dbReference type="OrthoDB" id="5376804at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021514">
        <property type="entry name" value="DUF3176"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35394">
        <property type="entry name" value="DUF3176 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35394:SF5">
        <property type="entry name" value="DUF3176 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11374">
        <property type="entry name" value="DUF3176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4376379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="444" mass="48035" checksum="D6566A2984FB04F3" modified="2023-11-08" version="1">MTQSKSDAAVNPDQQADRAAETKQALLTTIGESDDASREHNQDLRWKTGLASGEDWWVWELAGLVISAVALVCIFVLLRRYDNKQQPEWAHLSLNTAVALLSTLGKASVLIAVSRGLGQLKWIWLFNTERSLADLQFRPSTTQIAHVTNSTSYKAVGPNIGGSLYYIDSSMKANLYGSILSPNATQQWGVPAFICSIGDCTWDPVATLAIRPLCSDITSMLETSCSIFNSTTEGELEPCFVSLNGSRTLSFTNDPSLGRISMNMTVTGLGTGSTLPPIVYDNQTFPVIRYVSAIGTAIGLPGTSPEPVIPLSHFVATECALEPYVVSVQPKVNKSVYSEVILDEWSLTNTFSEMNITSDEGLTFHPPWGPELGMGRDQPFTMSFTAWNSVGLAIIYMFNGSIQTAQPIGDGSGTSADRATGGTIVIVDQQVQYYPTSEVVLYGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHM0</accession>
    <name>A0A9W8YHM0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001677</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHM0"/>
    <dbReference type="OrthoDB" id="1028014at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053185">
        <property type="entry name" value="SET_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332:SF6">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040919218" description="SET domain-containing protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="141"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4376394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="441" mass="49854" checksum="28657C0AB47059D2" modified="2023-11-08" version="1">MKSMIRLSLLTGTLAFASEQVQIAGQQPLFHDACPSESIALDDNAIISNVAFHSNKTQTWQSLEESRTSASQIRDFPWTFRPECFSNENTTEPFCVFSDQTFASGRGIFIVGTETLAYSMLEKDAFRRPEVLDRMNKYDNPSFEQHEFPGKGRGLVANKTLHRGDQIFASTPLLITDPDIYQLSTSERLALLYRGVETLPPASQASFWELMGHYDGDPVDDRINTNNFELSINGISHSGLFPEIAMMNHDCRPNAAYFWDARTMTHYVHALRDILPGEEITITYIDNEQPRAKRMKKLERNWGFRCSCSSCTAHPALTAESDSRLHQIAALLDMLNDWTPASSATPEAVELLVTLYEQERLHANLATAYKHAAEVYSSFGRKFEAIRYARLSTELSMLDKGFADEDVREMRNMSKNPEMSWSWNKRVGLSGKSGCGCARKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YI68</accession>
    <name>A0A9W8YI68_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_000616</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YI68"/>
    <dbReference type="OrthoDB" id="10031947at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052073">
        <property type="entry name" value="Amide_Lactam_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47171">
        <property type="entry name" value="FARA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47171:SF6">
        <property type="entry name" value="SPECIFIC TRANSCRIPTION FACTOR, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G06130)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="8">
        <location>
            <begin position="309"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="40"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="124"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="567"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="602"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4377786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="710" mass="78724" checksum="6B92B07E1A5B2270" modified="2023-11-08" version="1">MSTMITFAANNEFFRKRKRVHRACESCKKRRKRCSHTFDDEIEADGGSSSQSGHVPNPTSEPHAPAVNGHHYHPQDPRARGDSQGYGHHSTMPPPHSPAEDPHAATPPNFLGYLNPEAVLREQVHSERGKTAQSPSIAPIGQWIEERDQRPASTQGGSVSSRINETGPQTTQEVKVSTALQQYLDAVGVDLLPSRRNQEALLDIYFAYVHPLLPLVDKDLFYEQYEHGREPRMLMQSICIVASKHADAAKHLCLSPDPQPLSPREFSQRLYNAVIVGIEAKLEKNRVVLIQVLALISLHCEGPDGAEQASMHLAQAIHHAHTFGLQFGHQWKNQSSESQGNLEDVFWCLWSLDKINACMNGRPLLMHDRDNSLRQLPSDPEKRSSPFGIWLQISEMLDKVIDYYRPGRAAEETGWEGDDFLGFEEMVGDGEDKLDGPIMSLLSVFHHTMCMASHKSLSINAPVKSTPSYVRQSLSATRVIHLLNAEAPELLPPLPLVPYALGVALSVVYRHFRSRRLKVHVKRATEELKQCVRLLDRLRNAWWSAGTMADLGRAVLSNAAGRATNAVNTPATLSAEPRPHEPKTEPPTPKSTHSGLAPIPNPTQQWPQNLDTPIDPRLQQHQPPAPITSLLNPSPAPNPGQHPTSSERADRAPMPPAGFGFAEQSPDWLNFDAAFENFEGLLGSSGADLSNELFRPLNYETLEGFLDPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YI87</accession>
    <name>A0A9W8YI87_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YI87"/>
    <dbReference type="OrthoDB" id="2209940at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072380">
        <property type="term" value="C:TRC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.150">
        <property type="entry name" value="Haem-degrading domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005624">
        <property type="entry name" value="PduO/GlcC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038084">
        <property type="entry name" value="PduO/GlcC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010371">
        <property type="entry name" value="YBR137W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28255">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28255:SF1">
        <property type="entry name" value="UPF0303 PROTEIN YBR137W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03928">
        <property type="entry name" value="HbpS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143744">
        <property type="entry name" value="GlcG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4378181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="105" mass="11774" checksum="B922946121C84AA5" modified="2023-11-08" version="1">MCSLDNDFWVARKRNTVIRFGASTWRLHNVFDGGDEEKFKAKNGLGEKASEYAIHGGGVPVYVKNVDTPVAVVVVSGLKQWDDHMVVIEELAKVCEGLERKRQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIP0</accession>
    <name>A0A9W8YIP0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIP0"/>
    <dbReference type="OrthoDB" id="2823490at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="358" mass="40888" checksum="29C21B4C55E1CC91" modified="2023-11-08" version="1">MVSKLRKFLDEFEVRQKTSAISSNSGPPSEQQESASLTQITPDITMLSFMVLPRELRDKICTYVLFSQTNECPDLNQTFEQLLANRTCYDKPRLKSWLDLTMSHPGDTLSNATNLLLVNHQLHAETLENIKRYGKSITYDLDIILLDEILLLPTWTHVPILTTHLEEVNVTFRISGKYDAAKELPGRKRKDDDPAPGPYAHFGRWKGFRIGCGAGYAMGWQIYSILERFIRAGPTGNGTAADEHRHIAVKTLNINVETLADIDPARFGPHLINFLEGNIGSLLYGTNHEWFEYGQILYEHVDEVVVLKDGEELRRFDVAAMLESVVSIDKYLTQERLDSYKESTWMARRKRDLKAQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJL2</accession>
    <name>A0A9W8YJL2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ARF GAP with effector function(S)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">AGE2</name>
        <name evidence="4" type="ORF">N0V83_000544</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJL2"/>
    <dbReference type="OrthoDB" id="10266696at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08839">
        <property type="entry name" value="ArfGap_SMAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000010">
        <property type="entry name" value="Stromal membrane-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051718">
        <property type="entry name" value="ARF_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044732">
        <property type="entry name" value="ArfGAP_SMAP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705:SF1">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="3">
        <location>
            <begin position="16"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="153"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="463"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="197"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="303"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="359"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="414"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="465"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="508"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4377715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="543" mass="56797" checksum="8F2DB8F680098057" modified="2023-11-08" version="1">MSRRPNPAADRAEQNRTTLKNLVKLEGNKTCADCKRNKHPRWASWNLGIFICIRCSGIHRGMGTHISKVKSVDLDSWTDEQLQSVLKWGNGRANKYWEAKLAPGHVPSEAKIENFIRTKYESKRWVMDGGIPDPSTLEADDDDMPLKKVQEKVQIERSASQRLAGSSSQPQPPLARPQPAIDLFGDEPAPPRPSTGPPAGARAPPKAEAQPPKQTKAGDSLLGLDFLGGPTSTQPARPSSTGPAAAAPSRPDLKQSILSLYASKPAAPPQQPQHARQESFGGLVSPQTQTSPQQSAFGGMNDAFSSLSFSAAPAPKPSPFSGLDSFSSTKSPPSTSSSNMFGGGGNFFNSKPTSPPPSQAQHRTTSPSGGLGNFSAFTSPGPSKPATAASGMNDLLDLSMPTEPAPAPRVTSPPAASQQFNSAFNLSSPSAAPVPPAPKANPAPATVNYGGFSNMDAWGSNDVWASAAPSQPATTTTASHVAPKSPGWGAPHVAQDDDFGGWSSAAPTTTTHTSTTTHTTSTQNKPAGGFGGGSDDLFSNVWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJX0</accession>
    <name>A0A9W8YJX0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">D-aspartate oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">IFG3</name>
        <name evidence="8" type="ORF">N0V83_000454</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DAMOX/DASOX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJX0"/>
    <dbReference type="OrthoDB" id="2015447at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003884">
        <property type="term" value="F:D-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006181">
        <property type="entry name" value="D-amino_acid_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023209">
        <property type="entry name" value="DAO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530">
        <property type="entry name" value="D-AMINO ACID OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530:SF11">
        <property type="entry name" value="D-ASPARTATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000189">
        <property type="entry name" value="D-aa_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00677">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="7">
        <location>
            <begin position="59"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000189-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="348" mass="38352" checksum="2BAB9B3C4F0440C6" modified="2023-11-08" version="1">MPAFVPGKAKTKVGAISKLTYEGKATWIPTAFVDSFRAEVVLLHYAKQELNLLYGSNDNALRWDRWGYAHLSQLASERSKEAYVQWTPSTELWDDDGPRDKIQHMSEYLKDFRVLSDAGLSEGVKFGVSFMTLTVNAPEHIKYLYHRLQGQYGVRFSRQKLSCIESASDSPSTKIIFNCTGLASGTLPGVADPKCYPTRGQVLLVRAPHVKTNIMRHGADYETYVIPRPGSNGNVILGGYMQKGVGDGSTYASEAESILARTRALCPEVCQEEVEVLAAFAGLRPSREGGARVEREEILVGGRRRTLVHNYGAGGTGFQAGFGMAVESVALVKDILEETRRDVPQARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHD1</accession>
    <name>A0A9W9CHD1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sporulation protein rmd1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RMD1</name>
        <name evidence="5" type="ORF">N0V83_009893</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHD1"/>
    <dbReference type="OrthoDB" id="18302at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003734">
        <property type="entry name" value="DUF155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051624">
        <property type="entry name" value="RMD1/Sad1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF15">
        <property type="entry name" value="SPORULATION PROTEIN RMD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02582">
        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="488"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="DUF155" evidence="4">
        <location>
            <begin position="292"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="226"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4363597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="517" mass="57707" checksum="AAF9EABBA7BCB9EE" modified="2023-11-08" version="1">MASSSTETTPLISSMASGSRAVPPKPQRTVTFNPTISSASPKQPSRNLSFGPSSSGFQPTSHGGQPMLSSLNSKLRRRNSSGAPTRPPSGPAPKIGPQRTTRTAQKLKLLPDPAAEDGEDEESGRDVYVQYTRIKDPTARRDAARLGKADRERLPRVTAYCTAASYKLDDLMRFLKGKQKARGAMPKRFDECIYSPYNYGSKGPEDVASTSVLDGMPGGVQRRFSDSAIEVEHENERRRQDLIDLHDEGDAPNIGNEEAIDDRPRRPSITHADSDPQTDTPDFDVQVHTPEVFLFEYGTVVIWGMTLQEEKRFLKEIAKFETDKLGKDEVETEEFNFYYTREYQARIYNDFISLRDKKNYMIKLAISHGLSQSVKTSLFEDLVDNTIDDTKDIPAQIASSGTINLSKKQINMQIGELFILRISIHLQGSVLDAPELMWAEPQLDPVYQAVRSYLEMDQRVSLLTERLNVIGDLLAVLKDQLTVTHGELLEWIVIILVFAEVVVAAINIFVDLHAGAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHU6</accession>
    <name>A0A9W9CHU6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NDT80 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_010294</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHU6"/>
    <dbReference type="OrthoDB" id="2288358at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1390">
        <property type="entry name" value="NDT80 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052605">
        <property type="entry name" value="Fungal_trans_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024061">
        <property type="entry name" value="NDT80_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037141">
        <property type="entry name" value="NDT80_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35144">
        <property type="entry name" value="MEIOSIS-SPECIFIC TRANSCRIPTION FACTOR NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35144:SF2">
        <property type="entry name" value="MEIOSIS-SPECIFIC TRANSCRIPTION FACTOR NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05224">
        <property type="entry name" value="NDT80_PhoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51517">
        <property type="entry name" value="NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NDT80" evidence="4">
        <location>
            <begin position="68"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="NDT80" evidence="2">
        <location>
            <begin position="68"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="220"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="330"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="393"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="237"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="353"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="402"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4363174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="512" mass="55390" checksum="AA1002CB729E5A32" modified="2023-11-08" version="1">MSSTRRMSSLHDNSSSAMLPPTRSPGLNSVSPSMGGSVRDNYTTATPGLHRHPHTLSPRSDIPRYNTLPDTPRSVLPTAMPLTTYNGYQTGTYGGQPSSTETPPFNAQETYGTITCEGTTVTPNIDAKIEKGFFFSSDNVWTCYRRNYFAVNVSFNLTPWISNARLYYDQGNGKQQEQIQSMAVSLSAAVDGTAGKTIELIQHTPKRDKGPQLAMKKELLAPTPPGKSHEHGYGLSSFHQSSSLPGPQLPLQNDNDSSQQYSPTSHANSNYQHSFERIQFKSATANNGKRRAQQQYYHLIVELWANVQNPRDSEPRWVKVACRSSHPVVVRGRSPSHYQNEGPHNAGTSRGAPGAGLGGAGHHGLGSNGRASYSSYSNGLSGGGPTGMGGSMYRGNTYSLDPSPVGSHSVSSASSLSGGPIEGLVGDQHMVDDDDSKMMDAPHDYSYYPTPIYESIPPKLETTLPQPDRRIKDECPGPAAIAAGWQVGGCGRFQGMETSRGYYPDVHTHATY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIZ6</accession>
    <name>A0A9W9CIZ6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009294</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIZ6"/>
    <dbReference type="OrthoDB" id="103819at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF5">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="3">
        <location>
            <begin position="175"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="67"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="640" mass="70014" checksum="1C433C5212DC12B5" modified="2023-11-08" version="1">MDDGDDMDVSLDGGTSKRAQNKCEVKSDRRLLTKRSEKKIDRIEDRLSGIENVLEALASKLGNLDLRGDSAESSSQSRSSRTGAIADTGTPAPFEGETTINSQSDYAREFLAQAVGSTPSIGHNAEVKSALAALEEHLEVAMSQLDMFMPANYENIMALLLGAAHAIELCKPSLCWTLISSAAGLSQNLGYHRINTMMNDTVQDRRSKLHVFWMIYMFDKTLSLRLGRASLIQDWDISLPFIAPGDEALNGTEGSKMLAYWVKVAQVQGQTYEKLFSPAAFLRSPTERTRVAVDLVDAMNMAWYERGDASMGDLTNFSKSHTLHQQKATFTANSSPNETEVPSKRRLFGQQSGRAGASSDGKDHIHDHVQGSFEKVQDIFFHADVVMHYSTCALIQRAVTPDQVTFNQECLESSRAALVAHMKANSQFNTKGNEELWSGYIHWSILQAPFTPFIVIFCNAIQKADSSDLTSLTDFVTSLESCRTVSEGADKLYKMCRLFLQVAKLYISAKRQDMTPSSTTQTSSSYSQNNQPNYYTTADGTQLDLNAMTEFDPYLSALGLVSNNAWSMAGGPNVPSASMGTAAFSQAPAMGGVVGPNAAKMGFGPPGGGQNSIQDWFSGSRYLMNLMEAGDDLQMPDLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJL1</accession>
    <name>A0A9W9CJL1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">HTA1</name>
        <name evidence="16" type="ORF">N0V83_008372</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4 12">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the histone H2A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJL1"/>
    <dbReference type="SMR" id="A0A9W9CJL1"/>
    <dbReference type="OrthoDB" id="9421954at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000008">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0488">Methylation</keyword>
    <keyword evidence="11 12" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="14">
        <location>
            <begin position="10"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="15">
        <location>
            <begin position="94"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4365752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="134" mass="14136" checksum="35B522A89B04EDFA" modified="2023-11-08" version="1">MTGGKSGGKASGSKSSAQSRSSKAGLAFPVGRVHRLLRKGNYAQRVGAGAPVYLAAVLEYLAAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGHVTIAQGGVLPNIHQNLLPKKTAKPGKGPSQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJU0</accession>
    <name>A0A9W9CJU0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007771</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJU0"/>
    <dbReference type="OrthoDB" id="309640at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="628" mass="71864" checksum="84C00950AF9684D1" modified="2023-11-08" version="1">MSVFRNIKALFLGPPERPSSPERSKRKASSDFVRKTKYPRHDEPYSPRDYQKGSYAGSMTMTTTDEESGEDRARAGPSLSRNNPKTLKALLHEPKKQSSPTRVVSPDKIRKAAEARHLLSKAHEKYESIGNSEAERLGASIGRLETRAETMAKHLSTATQTRNGKEPATLIQEFQPLSDTSSEIDPNRNDGEDIYTPPSLHIGERSEGWEELTAQKKWQVKPNLRARELVAENLGRAKVAIEEEVEEPEYALRHAEVRDGMWQIMDQTERFAQEFFRFQFSPGKDGAVSNGWYQSLTPQTAKIIGCVASGGPAGIHGWHDLFINEQKRRALVCGIIGNVLVEQVFQHMFFGGRATDIQAVFALQEKHRNEDGFDRNKHYAVAIRKILMKNATSETLILPANFNNHVNQIVSALWTHLAPIFHLNSRGAYSSRIAEIPDDMFIKLHELIIQAGLLSLHMRLDPHTVYYFEPVFKEYNFTSQRMECFNSRDMQQRHPRYTPEELLTLDTKEQKRRANISDAEKKRAENDEPLTQITIMDGITAYRLGGWETADSSVLEPRYEKSEYRSQGSRQRILTHGWVYCRWGRARRYDRGRPNDDATIHGEAWKGGFVEFTDVDGVPNWSEMERDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJW7</accession>
    <name>A0A9W9CJW7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">NIK1</name>
        <name evidence="16" type="ORF">N0V83_007862</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10266508at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06225">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000235">
        <property type="entry name" value="Probable nik-1 protein (Os-1p protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000002">
        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1530:FF:000001">
        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1530:FF:000002">
        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000015">
        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1530">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18947">
        <property type="entry name" value="HAMP_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="HAMP" evidence="15">
        <location>
            <begin position="210"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="15">
        <location>
            <begin position="305"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="15">
        <location>
            <begin position="397"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="15">
        <location>
            <begin position="489"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="15">
        <location>
            <begin position="581"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="15">
        <location>
            <begin position="673"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="13">
        <location>
            <begin position="746"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="14">
        <location>
            <begin position="1116"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1255"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="153"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="10">
        <location>
            <position position="1165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4366226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1312" mass="144116" checksum="03692C73ABC227C8" modified="2023-11-08" version="1">MSAETYAGLADIIKNLAKQHDPSREPTFSAQIAANGAKNSIALPGPASEEKALLEQELAALCARVDFLEHKSSVVVNQNTFPVTPAGEPPEDGLGYTPTGAFRSSNGPPTHGRRGSNKERALWVSNWLAAKESNGASEEPAAALTEEQLNYLRDHLNKQADQIKNQREHIDNLSAEVNKQLTNQNIVFEHGIEDIGALKRELGKHQQANLAFQKALREIGAIVTAVAMGDLSKKVLIHAKEMDPEITLFKRTINTMVDQLQEFASQVTFLAREVGTEGRLGGQANLPGVAGIWAELTDSVNVMAKNLTEQVREIAVVTTAVAHGDLSRKIERPARGEILQLQQTINTMVDQLQSFATQVTKVARDVGTEGKLGGQAEIAGVKGMWNELTVNVNAMAQNLTTQVRDIAQVTTAVAQGNLTRKVEAECKGEILELKNTINRMVDQLQQFAHEVTKIAREVGSEGRLGGQATVHGVEGTWKDLTENVNGMAMNLTTQVREIAEVTTAVARGDLSRKVKAEVQGEILSLKITINTMVDRLNTFAQEVSKVAREVGTDGILGGQAQVDNVEGKWKDLTNNVNTMAQNLTLQVRSISEVTQAIAKGDMSRRVHVDAEGEIRLLKDTINDMVMRLDEWSLAVKRVARDVGVDGKMGGQADVRDIDGRWKEITTDVNTMAQNLTSQVRAFGDITNAAMEGKFTQITVEASGEMDELKRKINQMVSSLRESIQRNTAAREAAELANKTKSEFLANMSHEIRTPMNGIIGMTQLTLDTDLTHAQREMLTIVHNLAGQLLTIIDDILDISKIEANRMVMEEIPFSMRGTIFNALKSLASRANERKLNLAYDVSFKVPDYVVGDSFRLRQIILNLVGNAIKFTELGEVKVAISMAQEQECGPDQYVFQFAVSDTGIGIRGDKLNLIFDTFQQADGSTTRKFGGTGLGLSISKRLVTLMGGRMWVESDFGKGSVFYFTCRVRLGNPELTAIQPQLAAYKQHTVLFVDQGHTNFSGEIVEHLKTLDLVPMVVNTVEDVPDTAKRTDMPYDCVIVDNEKTARELRVAERFKYIPLVMLTPHVAISLRSALENGISSYMTTPCLPIDLGNALIPALDGRAAPLVSDHSKSFNILLAEDNAVNQKLAVRILEKYHHRVTVANNGLEAYEHIQKKRYDCVLMDVQMPVMGGFEATAKIREWERENGIPSTPVIALTAHAMVGDREKCLAAQMDDYLSKPLRQNQLIQTILRCATLGGNLYDHDTRHSMEHLPELQLPSDKAGGATPKKRPQLDARGFTERGNAADSPKLLAVDQTDSSSIERVRSLSASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK42</accession>
    <name>A0A9W9CK42_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK42"/>
    <dbReference type="OrthoDB" id="4158987at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="188" mass="20227" checksum="8AE5CF33BFEBAF81" modified="2023-11-08" version="1">MTSLHNGGHPPLHASVVCPILTRTYRTLPTITMPPKKLEQRSSKSPPQQDEDAPSQDDLKSLIASTKDSPESALQDAASKAEAALAAQKTASSLRSAADTIRDPAKREKYLQDAYKKEVEAHGNSKKARVLSSGAFRGQSGWRDWYGCWSGAGDGRGDCDRDCCDDSYDGGGGLWARGWVGFMGLGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK52</accession>
    <name>A0A9W9CK52_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007599</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK52"/>
    <dbReference type="OrthoDB" id="10674096at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="262" mass="29528" checksum="2DE1263EB009A1C7" modified="2023-11-08" version="1">MPLSDVKKRGKAEKSADKKPKSSRPSKTATHNIGRTLAGHVVSGDNGDDDDDSGDYVPPEKDEESDKDSDLQDVDEDELDPDVQESIDDEGNIVRIVGGKAMSDQEILDSIHASPTYRKMREFKKQRWVSQHKKMKSDAKDAEDDPDDEIIEVDCREDDDSGARYAMLPVTDVDDIRGGVFKTGLGYTFEGKRSVWEIDKMRLKVKYDSGEKDHMGRPVWKEKEQLAAKIFADGSIAVRKREKGQPEEEPNGQRSNKKKRSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK68</accession>
    <name>A0A9W9CK68_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_007327</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK68"/>
    <dbReference type="OrthoDB" id="4116913at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF39">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="3">
        <location>
            <begin position="278"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="336"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="347"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4366802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="511" mass="56804" checksum="B164AC0799C2091A" modified="2023-11-08" version="1">MPPLPTDERHISSTRDTRSLETPDFYRRQIKPAVPSERANGQALRLQQSIDELRALLSKNKDIHFTKSEFQDALPTTGKRKYNQISPYDELADQGSSKSRFKSDNLISLPADSQTFGTSSFYLHPMEPTDAKVAPPTMAQAKLQPTMRLANCASGSCFLPSPAEGSALLNEYLHDFNSRIPLFDPETVYNYVRDCYSGTADQYPLAWVLSYTALGIGHRLRAMSLFAATDDTPNAEFYLNKCLAVLPDLLLQEPTLELVQALLGVSILLQTSTRSRRAGPFVSTAMRIAQELGYNEVSQEQIEESSRDKAAYYVFWIAFFMDTGMNLRGMRPNTQKLGDIDVPLPNSSSSQQSSSTASETSTAGREVNVFALHASLSLIQAEALEELFSFRARQRPADSTTTSFEKMMIKLTTWRMNNPLADPDAPSILDSLYRSDVVHSVILEASYFATLYQLHASKVLGAFRSRVDVFSPDGLRSAAGKISHEIFADAHRLLEFASLVPLGDVSVTWYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKC2</accession>
    <name>A0A9W9CKC2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Component of the SF3b subcomplex of the U2 snRNP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">BUD31</name>
        <name evidence="4" type="ORF">N0V83_007376</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BUD31 (G10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKC2"/>
    <dbReference type="OrthoDB" id="277109at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001748">
        <property type="entry name" value="BUD31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19411:SF0">
        <property type="entry name" value="PROTEIN BUD31 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19411">
        <property type="entry name" value="PROTEIN BUD31-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01125">
        <property type="entry name" value="BUD31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00322">
        <property type="entry name" value="G10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4366848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="141" mass="16528" checksum="2D67B54D517A61F7" modified="2023-11-08" version="1">MPPVRTARASKKAPPEGFEDIEDTLLEFQNKMKDAENASHEGKKKYEMTWPVFQITHQRSRYIYDLYYEKEAISKQLYDYLLKNGYADAMLIAKWKKQGYEKTKETNFRSTCICRVPKDQMKENQEIQCVNCGCRGCSSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKH2</accession>
    <name>A0A9W9CKH2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Endopolyphosphatase</fullName>
            <ecNumber evidence="3">3.6.1.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PPN1</name>
        <name evidence="13" type="ORF">N0V83_007095</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the endopolyphosphatase PPN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.10" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKH2"/>
    <dbReference type="OrthoDB" id="348678at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000298">
        <property type="term" value="F:endopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004309">
        <property type="term" value="F:exopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006798">
        <property type="term" value="P:polyphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00842">
        <property type="entry name" value="MPP_ASMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000082">
        <property type="entry name" value="Endopolyphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041805">
        <property type="entry name" value="ASMase/PPN1_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012358">
        <property type="entry name" value="EndopolyPtase_N1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340:SF55">
        <property type="entry name" value="ENDOPOLYPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027093">
        <property type="entry name" value="EndopolyPtase_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="549"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="656"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="708"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="550"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="716"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="765"/>
            <end position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4367512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="822" mass="92956" checksum="A7BFF5531E414D2D" modified="2023-11-08" version="1">MHFGESRATPTRTFAKTFAPVALLTPLSDAAPVMSASRQALICGLLFWGSARAAPPSPAGGAGESAAGTSEASRSVSAARKLTGRFLHVTDFHPDPFYKTYSSTTADAACHHDHGPAGIYGAETSGCDSPFSLINQTFQWINDNIKDDIDFVIWTGDSARHDNDEEIPRTQKQVIQQNEYMVSKFAEVFGQNNHHHGSMGDFVIPVVPTFGNNDIMPHNIFLSGPNRWTMKYLDIWRSFIPEAQRHQFQQGGWFSVEVIPGKLAVISLNTIYFFNSNSAVDGCANKHEPGYEHMEWLRIQLQILRGRGMKAILMGHVPPARVDSKESWDETCWQKYALFGERFRDVIVGSLYGHMNIDHFMLQDFADINKDTKKGRMADTGKLQSAGSDPIALLEDGEVTVASASDYLLDLRQAWAQLPAPPAKSNKSLRPKSWSEKLSMFEWLFSKTNRPTKDSNVGRSEKKRYLDQIGGKYAEHFAVTHVAPSVVPNYFPTLRIIEYNITGLEHILMPSVSAREDFGSWDRPSKQLSPVENELLDDVEYLRDVDTQVKRKQKAKKPKKRPKKYKFKVPEGPSKSAPPGPAYSPQVFTWTRYIQYFANLTQINNDFVDPPQDLSDPAKPHSQVHFEATTVNDLSPHTIFGLTIGSDGEIEHNKWKEGKHGKHQGKRPRPEPHPKEFVFEVEYDTKKDKGFKDLTVRRWVEYARNIGQGSAKTADVEEYEGDEEDTWDDTEEEEYDEEPVADDASDEDDEESEAEEEYDAESRAGGHKKHKKHKKKKKHHASKDWFTFVKRAFVGTMDPHEIKELFGARSQANEVAEEIMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKL4</accession>
    <name>A0A9W9CKL4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006961</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKL4"/>
    <dbReference type="OrthoDB" id="3796409at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37540">
        <property type="entry name" value="TRANSCRIPTION FACTOR (ACR-2), PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37540:SF5">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4367379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="506" mass="56864" checksum="946C9951C53F7BF0" modified="2023-11-08" version="1">MSQKGRGRQFFQQLQPTFSIELPKESATRLEFLDHSDPSKDTVVRKKAREWVNKNKANSKKSRDVQVFSEAKRTAGVINEADETQRQLYMKRSKWPEISSNPFQAIGNLDPFNLLPEVGRKYDHIIQFFLTSSCPEEIPCSDDKYSDQSKHSLIPFSSDNTVLGNMAKSQATFILWLYATALIRDGMCGSINTEEVQWFYNKSLRALQETLKKDVESGEYSDPLLNCLACMTATASFAGMFKTAELHRDALIKVLALRGDGDVVQGIKSATTWSVKAIQWCEIMVATQLVELPKIPYLPFAPAKPAPKQVTLEAARLTSITLASLPRLSESLHKIIHLLHHLGIAYAQPTGTKIDSYIIQPLYDAEYALLQLLSSQKSPGHGYSEIEVLLAEAFQLYFWTGPRMLPPQTRLCDLLITRLSITTKRVDGIQSIKETCITTVRILKDVHMGQLQSSLRNALSTFGPDSAQYRTIKLMVDEHAAKLALENLTLSSSRAKQQDDQKMQMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL45</accession>
    <name>A0A9W9CL45_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005855</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL45"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="155" mass="17884" checksum="FA89DF5EF62AB943" modified="2023-11-08" version="1">MSKFTPLNQEIPVLQSPPNSPFNPASKSEEIKRINDLFLLAMKQLQDLKHLYLGPGTWEESQSGGKEKYRTRLQESERKFYVKSVMRLHRYTEDILNWTIFVDRDLGAPNEWEYLMARPDREESGESGEDESDAQEEEAEEAEEQDGGLSLVGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN21</accession>
    <name>A0A9W9CN21_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MIP18 family-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_003914</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MIP18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN21"/>
    <dbReference type="OrthoDB" id="2746at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000007">
        <property type="entry name" value="Cytoplasmic protein required for cell viability"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039796">
        <property type="entry name" value="MIP18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="4">
        <location>
            <begin position="71"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4372141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="197" mass="21619" checksum="D0BE50548131A563" modified="2023-11-08" version="1">MDVDKDNANPTILNPSDLPSRRRESTVKGRDDGGTGLFDDLIPRYNYLSDPFDEPVSPSDSDEDARDDIDEQEIYDLISTICDPEHPLSLGSLSVVNLPDIRILPPTSPLSSISTVVVEITPTITHCSLATVIGLGVRVRLEQALPPRFRVDVRIKKGTHSTDEQVNKQLGDKERVAAALENGTLMGVLRKMLATCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNN3</accession>
    <name>A0A9W9CNN3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_003798</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNN3"/>
    <dbReference type="OrthoDB" id="93019at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051178">
        <property type="entry name" value="TfdA_dioxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43779:SF2">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT XANTHINE DIOXYGENASE XAN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43779">
        <property type="entry name" value="DIOXYGENASE RV0097-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="7">
        <location>
            <begin position="20"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4372025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="375" mass="41907" checksum="C03C7D35FC60B59D" modified="2023-11-08" version="1">MPHATISPITVHKIEPQPGSSFDFGAEIRGADLENITEADASAIINALYENQVILFKNQHTLSPKAQYELTRLFDPSATGYGHGKTLDAKRSILHPDLKTIPHQPQVQVIGNGPVAEFEGLKNITLKHPHHRTFHKEAISDEDDRENTRFYRWHIDAALYDLEPPKVTSLLAVKVPKTEFQTLRYDDGTGDEVQLSRGSTAFVSSYKMYDLLSEEDKAFVRGTKVQYAPHPYIWMSPARSRSDGLGLISDNLELPNDQLPPIDESKIKILPMCWKNPVTGKLALQVHPSAVQALHLADGTIISDLKEVREIVHRLQRPGIAPELVYAVNWEEGDMALFNNRGVLHTVTGSFLPEEVRIFRQCNMAASEGVVGPDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNN5</accession>
    <name>A0A9W9CNN5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sphingolipid C4-hydroxylase sur2</fullName>
            <ecNumber evidence="8">1.14.18.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SUR2</name>
        <name evidence="8" type="ORF">N0V83_003628</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.18.5" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNN5"/>
    <dbReference type="OrthoDB" id="408954at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102772">
        <property type="term" value="F:sphingolipid C4-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="7">
        <location>
            <begin position="205"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4373334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="437" mass="48924" checksum="80F83C5FA02BF804" modified="2023-11-08" version="1">MASANTTSYDLPPLPEYTLTPLQPLTSWVSDALIQAALPVLGYWIVSLIFHAIDVYDVFPQYRLHTPAEVLKRNHVSRYEVLRDVVLQQIIQIIASFSLSLFDEPLTTGKADWDVSWYAQKIRLAQRAVPLILSAVGVNPVALASNMSSSYPTLSSVLLGGKYPALLQTATIAGEQTLVPAFAAWELATASFVYWYGIPAIQFSAAIVIMDAWEYMLHRAMHMNKWLYVTFHSRHHRLYVPYAYGALYNHPIEGFALDTLGAGLSYLLTGMTMRQSMWFFTVSTVKTVLDHGGYAFPYDPTHWIFPNNAAYHDIHHQSWGIKTNFSQPFFIYLDRWCGTMYKGDVTEKYERARRAAQQKMDQEKENGVTSVPAGSVAASAIPNDESLLPQGVATPRMSRKKASSISSSAGNFKDLTNKVNQNLHGRRANVLGVRSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNT3</accession>
    <name>A0A9W9CNT3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Flavodoxin-like fold protein</fullName>
            <ecNumber evidence="5">1.6.5.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PST2</name>
        <name evidence="5" type="ORF">N0V83_004113</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WrbA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.2" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNT3"/>
    <dbReference type="OrthoDB" id="504689at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000001">
        <property type="entry name" value="NAD(P)H dehydrogenase (Quinone) FQR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010089">
        <property type="entry name" value="Flavoprotein_WrbA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01755">
        <property type="entry name" value="flav_wrbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002999">
        <property type="entry name" value="PRK03767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546">
        <property type="entry name" value="FLAVODOXIN-RELATED PROTEIN WRBA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546:SF23">
        <property type="entry name" value="FLAVOPROTEIN-LIKE PROTEIN YCP4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="4">
        <location>
            <begin position="5"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4372339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="204" mass="21917" checksum="E3D6B197102AC218" modified="2023-11-08" version="1">MAPKIAIVYYSMYGHIRKMAEAELAGIKEAGGDAELFQIAETLPEEVLQKMYAPPKPTDVAVLKDPAQLEPFDGILFGIPTRYGNFPAQYKAFWDASGKQWQQGSFFGKYAGLFISTGTLGGGQESTAIASMSTLTHHGFIYVPLGYKNTFADLANLDEVHGGSPWGAGTFSAGDGSRQPSALELRVATAQGKAFYETVAKAHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPD0</accession>
    <name>A0A9W9CPD0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003509</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPD0"/>
    <dbReference type="OrthoDB" id="5422351at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="452" mass="50016" checksum="0B3D1851710CC67F" modified="2023-11-08" version="1">MPSTPIFADFKVLKEKLASKRSRSGSKPKELEIGSPVLISTTNEELNLIPLPVYQPVPAPSSRVPSPATTPTIRPVPTIKKEFSPLASHPVDPDNDSLFEFIESRTAKECAIRRRRSHVISTVVTSEFKIDQGRSRANSADTRRTRHYLFSNDPWLSSPKLQEEFDMQDRFVADDTPSAPILSRPSSFLGLPTSSDRPTSSHGGHRSPNLRQSPLDKELPALPRFLTPAPLFACNSSSSTPVLTEEPQDELEEDENTECLHELQMQFQEKPRSHFSTWSSDSIAYSCPASDDDAVQSPTFSSLTSDCSDIDTPQDQSVRYSYVEQTSSSDDDKRTPTIQAIDTDSSSSDEANTTYLSATPPQLDNLLRISTFGSDLFSLDIQHTDTTPRRQAACFGLGFYTLPDDETTSKTTITDADVSLHNAPTVNVQRESSMSQLNKLMDEFAFLGDAVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPD2</accession>
    <name>A0A9W9CPD2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase S33 tripeptidyl aminopeptidase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_003373</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPD2"/>
    <dbReference type="OrthoDB" id="425534at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013595">
        <property type="entry name" value="Pept_S33_TAP-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051601">
        <property type="entry name" value="Serine_prot/Carboxylest_S33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248">
        <property type="entry name" value="2-SUCCINYL-6-HYDROXY-2,4-CYCLOHEXADIENE-1-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248:SF25">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08386">
        <property type="entry name" value="Abhydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040796217" description="Peptidase S33 tripeptidyl aminopeptidase-like C-terminal domain-containing protein" evidence="4">
        <location>
            <begin position="20"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S33 tripeptidyl aminopeptidase-like C-terminal" evidence="5">
        <location>
            <begin position="477"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="457"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="457"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4373082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="591" mass="63828" checksum="77C3AD6891B0FD31" modified="2023-11-08" version="1">MSRIASFLAAGLAATAVTARPTSERWTRQTSSNTSMIYSFEDLPSSADLEWTPCYDRFDCANLEVPLDYAEPDIGTTIIAFIRQEATNGTGHDILFNPGGPGGSGVDYILSGGGDKIIELTGGKNGVVSFDPRGVNNSGIALTCFPDDPDARDAYYSSMNTDLPTLNEDYYQAIALGQWCTQANNGTYARYAGTSAVVQDMMHFTELQAALNGDEKPEESQIWYYGASYGTVIGHTLAMLYPDRIGRIVVDANVNSENWFNGLVRTSVETSDDGYDWFFKLCAEAGLGKCAFAGNSSSGDDIKKRFDSLLTRLEKAPVVSNDTTSPSGPQIITKSRVLNVGFGTLYSPVSQWPLLAAGLYFLEKDNATGWIEVEQALSEPTNPGPFNYTSIAAQETLTFITAVDAAGRYPIKDVDDFIKVAGEIEKESVYAGENYVETNILINAGLKLSPPKSQYFSGKSSQVKSRPSSTHPSDSFLCERKPANDGQKGFKQTNTSTPILFINTSADPITPLASAKHMSTYFPGSVVLVQNSPGHSWTAIGSSCVEGHLASYLADATLPKEGTVCETDTKPLVDTKEVAKRSLAVNGHFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ68</accession>
    <name>A0A9W9CQ68_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">FACT complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SPT16</name>
        <name evidence="17" type="ORF">N0V83_003128</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the FACT complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase M24 family. SPT16 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ68"/>
    <dbReference type="OrthoDB" id="10251642at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000002">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.210:FF:000001">
        <property type="entry name" value="FACT complex subunit spt16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000017">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.350.10:FF:000006">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000005">
        <property type="entry name" value="FACT complex subunit spt16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.210">
        <property type="entry name" value="FACT complex subunit Spt16p/Cdc68p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029148">
        <property type="entry name" value="FACT-SPT16_Nlobe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056595">
        <property type="entry name" value="Fact-SPT16_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048969">
        <property type="entry name" value="FACT_SPT16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013953">
        <property type="entry name" value="FACT_SPT16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040258">
        <property type="entry name" value="Spt16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980">
        <property type="entry name" value="CDC68 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980:SF15">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14826">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24824">
        <property type="entry name" value="PH_SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08644">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21091">
        <property type="entry name" value="SPT16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01285">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01286">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 11" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FACT complex subunit SPT16 N-terminal lobe" evidence="14">
        <location>
            <begin position="7"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="FACT complex subunit SPT16 middle" evidence="15">
        <location>
            <begin position="540"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="16">
        <location>
            <begin position="813"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="446"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="753"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="927"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="619"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="472"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="935"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="950"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="962"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="994"/>
            <end position="1007"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4374387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1021" mass="115619" checksum="3D9ED271E6799198" modified="2023-11-08" version="1">MADDIVIDKQAFQERLGSLVTKWKADKRSGDQIFQGAGSIATVVGKASEPGSYLKPAAFQLWLLGYEFPATLFILTPDLLQIVTTKKKASYLEPLKGGKVPVEILVRGKDAEENKKQFQTCLDTLKKAGKKVAVLKKDGATGAFADEWKAAFQEAAFKDEDQVDLGPILSNAALSVKDEKELRTIRDASRASSALMTNYFVEEMSDILDSEKKISHRALADKVANKIDDAKFFEKQKVSKTFDSMQLDWCLQPTVQSGGTYDLKFASEPDDNNLHAGVIISVLGLRYQTYGAMVGRTYMVGPNKEQETAYKLLLAIHDLVIKSIKDGVVAKDVYNKALAHLKSKKPEWEKHFPKNVGYGIGTENKDTALLLSGKNLRVLKDGMTLVVQTGLQDLENSKPQDKKSKTYSLVLVDTVRVGQGDCAVFTKDTTSDLDAVSFFFDEEEEEAKPKVKKERAPAIAQTNITKTRTRHERTTNQDAEKEEQRRQHQKELHSKKQQQGLEQYSEGAKSLNGTEEKKFKKFESYKRDNQFPNSVANLEIVVDKKNLTVLLPIMGRPVPFHIHTIKNASHTPEADFTSLRINFLSPGQGVGRKDDQPFEDPNAHFIRSLTFKSQDVDRIDQITKDITELKKDVVRRETEKKQMEDVVEQDKLIMMKTRKPHMLDLIFIRPALDGKRIPGSVEIHQNGLRYVHGNGSQKIDVLFSNMKHLFFQPSQHELIVIIHVHLKNPIMLGKKKTKDVQFVREATEMQFDETGNRKRRHKFGDEEEFEQEQEERRRRAALDKEFKNFAEKIADAARNENVSVDIPYRELGFNGVPSRSSVLVQPTTDCLVQLTEPPFTCLTLSEIEIVHLERVQFGLRNFDMVVVFKDYNRPPVHINTIPVESLDPVKDWLDSVDIPFTEGPLNLNWATIMKTVTSDPHQFFADGGWSFLSTETDDEDDGEEEEESAFEVSESELAVSDESSDESDFDENASEEMSDEGSEDEFSEGESWDELDKKAARKDKESALEDDDEGKGKKRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQL2</accession>
    <name>A0A9W9CQL2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Synaptic vesicle transporter SVOP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NAG4</name>
        <name evidence="8" type="ORF">N0V83_002212</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQL2"/>
    <dbReference type="OrthoDB" id="6770063at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF48">
        <property type="entry name" value="MULTIDRUG TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G02700)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="367"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="447"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="472"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="507"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="540"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="138"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4375128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="588" mass="65117" checksum="AD74623A5BD3837D" modified="2023-11-08" version="1">MAEEAIIQPSLTRDFASPNPTHPTHTNTNALTSDAASDVTRVDPNHQTHHEPTTIHRTATTTTIHRTPTIEDVVNLPLRTLSNEANLDEYTQETIDGQILREVRSAANGKIERYELVTFKVDDSENPKNWSKAYKWWCTMCVAVTCFAVAFNSAVITADLEGVSKEFNVSEEVSLLTITLFVVGFGVGPMAFAPLSEIFGRRPVYAVTLALAVIFTIPGAVAQNIGTLLVTRFLAGVSFSAPMSLVGGTLVDLWRNEERGVPMAAFSAAPFIGPGVGPLVGGFLGDAKGWRWLYWIQLIISGFVWILITFTVPETYAPKLLADRAKKLRKETGDDSFVTEQDLDMRPFSQRLSLFLLRPFQLLFRELIVFLISVYMSVLYGLLYMFFVAFPIVYQKGKGYSASSTGLMFIPLIVGVLLSAACAPLVNKHYLKLCEKHNGSPPAEARLIPMMLSCWFIPIGVFIFAWTSYSDLHWAGPAFGGFPVGFGFIFLYNSANNYLVDSYQHQAASALAAKTFLRSFWGAGAVLFTNQMYDRLGDQWASSLLAFIGLACCAIPFVFYFYGAKIRARSHYAYSPEAEANKSEKQEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRA9</accession>
    <name>A0A9W9CRA9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DUF221-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_001507</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRA9"/>
    <dbReference type="OrthoDB" id="1076608at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022257">
        <property type="entry name" value="PHM7_ext"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF20">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN 75"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12621">
        <property type="entry name" value="PHM7_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="685"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="729"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="776"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="825"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="898"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="937"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="968"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="11">
        <location>
            <begin position="30"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="12">
        <location>
            <begin position="231"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="12">
        <location>
            <begin position="562"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="9">
        <location>
            <begin position="684"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="domain" description="10TM putative phosphate transporter extracellular tail" evidence="10">
        <location>
            <begin position="1167"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="422"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1051"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="357"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="489"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1051"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1071"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1096"/>
            <end position="1120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4376224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1247" mass="140453" checksum="BF5F968EC14984B6" modified="2023-11-08" version="1">MSATAASASPTSTSGTGSAQKAQNQSIENFAASLSTAAIIFGVQLTIFLILSGNWKLHKTKASTNDKSDERQSFFSIGDQSNANETSYYKTSFVPQAKRIAAPVTAIESFKNVFTISDRELVRVAGVDGYLFLQYLQLLLRIFIPMALVILPILLPINRSGDVKGVSGLDSFAWPNVGVPAKVHRLWAHLILAVCVVCWVCFNFYLALRKFIRLRQTILTLPEHRIRASATTILVQSIPRKWLTVPALDALYDVFPGGIKDIWINRDFDDLMTKVKKRTKIARKLEAAETDLIITCTKKHKEMEEKAAKAAGKQRTKKEKKHDEALQNEVINQETHEQGVDAGNPHQIEEQLQQVLEVDSVSSSSSSSRSTSPDRKRGLIPIPFVGQGIHAVGEGIRDVGQGVDRFNKKIYGGVRGVFGGKQHREHRHEHESAEPPQLDGAAEVPGSSPTSADFAHRGASSDTYTPLAPPTKEKYGSEGTVTEPNSPKPAERIDTTEEKEKPKKGGFRPWQKLVASYFGQGDFASPQPFRQEGEVLPIEVKDEKVNYDMIGLYDDAFAEDGEDAVWRKYIQSKDRDTMRIPLFDKSWWPSLPLIGKKVDTIYYCRKELARLNVEIAEDQAHPERSPLMNSAFIQFNHQVAAHMACQSVSHHIPRQMAPRTVEVNPNYVLWDNLTMKWWERYLRMLAVILIIVALVIFWGIPVSFTGALSQVKTLTDELPWLGWINKLPTWLISFIQGVLPPAFLAILFAVLPITLRFLAGVTGTTTSGERELLVQNFYFAFVFVQLFLVVSISTGITTAVQDLVNDPISIPATLAQNLPKSANYFFSYMILQALSISSGTLLQIGAVVVIIFLRFLDKTPREKVSRVLSRPGINWGTMIPVYTNFGAIGIIYSVVSPLILIMMLITFCLFWFTYRYQMIYVTYAKAETNGLIFPKAVNQLFTGLYFLELCLIGLFFLQRDTNNEVACFPQAIIMIIALAATVLYQFVLNRAFGPLFTYLPITFEDEAVERDEEWQRAQASRWEKNNEEHEPLTAEQDAKLTAEEREMAHLEEQNRLDKKQRESSYGPGSYELSNLDSTRSTARPTNSRHSSNWAQRSRRSSNSRSRSHSHAHTPKRHNKRKDPLDLVTSTLRRGLDDVTRPVRDIEAQILPSKNLFDDIDDELADIEPEARQKLIKRTFQHPATRAIQPAVWIPHDNLGVANDEIQRTSAYTKKVWITSVNARLDASGNVLYRGLPPDRDPFENIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRU0</accession>
    <name>A0A9W9CRU0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA repair protein rad9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRU0"/>
    <dbReference type="OrthoDB" id="60092at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071479">
        <property type="term" value="P:cellular response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026584">
        <property type="entry name" value="Rad9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007268">
        <property type="entry name" value="Rad9/Ddc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15237:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT CONTROL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15237">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04139">
        <property type="entry name" value="Rad9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009303">
        <property type="entry name" value="Cell_cycle_RAD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="39246" checksum="081AC8F4AF3EC7C5" modified="2023-11-08" version="1">MYTKALLSVFKGRLYDPLGRDGAIDRCEVSVQERENEAQCRFIVKMVCNQGVIKTYKLTYEAVDVMHALFDRSVAKNRWSMPSGAVKEYIEYFGTKTEMLDIYAGEDGRCVFKSYTEKISNGKEILKHPLVTAVAVSTSDFDEFSVQAGLHIIISVKDFKAIVIHADTLKTSLKAYFSQPTRPLQFSYGCDGLLCEFTLMTSGDHSAAPPTPTPAPQTLESRQTSRAPSISTDRAESRNVRSDMPPPRRVRNPGSRQKPSTAQSNPAQHNSESLFIPDNEEARRREDEEDSAWAPINYEKEETLGWDASADHDASVFPTFRDSGSMSGTNTADSNEVFEPTQRVSQIKGLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYQ8</accession>
    <name>A0A9W8XYQ8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_009669</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13 14">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="952271at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2230.10">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF24">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00695">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DUSP" evidence="10">
        <location>
            <begin position="217"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="562"/>
            <end position="1372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="419"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="522"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="936"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1048"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1387"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1443"/>
            <end position="1490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1517"/>
            <end position="1595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="36"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="99"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="153"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="169"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="530"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1059"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1070"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1094"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1113"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1412"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1578"/>
            <end position="1587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4363376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1595" mass="175141" checksum="019AADB28A688FC5" modified="2023-11-08" version="1">MSRVTTASNNSKKRKIVTPAPNSPRDETAAPSPNVGAGAASASPRSSPHPSASPPRYLPPHLHDEVLVEPSHDAASAAARDSYTGASSPSEAYANLTLDSREHSMTESDASRPEQQQQQQQQRRPPRSSSPAKRLHSDMDDSSMDVDPSASSTRRNSGQSSPRATKPLAAAASQRSARATSVEMADAPNNSGSSETDASNADSSATSVDASQQADLPSLDEQVARVMTMMGGELQDRQEGYVISEKWLERVWARTSENLSRPQDFSKEATQGPIGPVDNSGLVDMEALQEDLADQRGDDFIPLIKGTVIGQDFEVLPAKAWELVVGWYGLKEGSPVIRRYAHNTVPDKSSENIIYELFPPIVTIRKVRKSPPTGTENTRTAERMVASRYDSFLGFLEAAKKAASIDLNNKVRTWRILKTAPSDEPQPSQPSGILTPDASPRDGSPVSSQRAPLIMNVTSFSSLTYGVERELVTGKDEKANEEFNDSLTLADAGITQDQVIVLEEHDEEGKYISDSVRVAPKKQAGAQANKALQSNPNSGRSTPTSGALTRGRTRTGKVRGHVGLTNLGNTCYMNSALQCLRSVEELSMYFLNKKWQEEINTTNPIGHKGAIARVYAQLLTQIYDVGNMSSFSPKNFKQTLGKANSLFSGYGQQDSQEFVSWLVDAIHEDLNRIHNKPYTENPDSDDNTFRDPELIKQLGETYRANHRARNNSVATDLFGGFYKNTMVCPVCDKVSITFDPYSQLTLQLPIEQTWTHTVTYVPLYGKPFQLEVDIDKNASIKTLKEFVGKRGGDVKASHLMASEVYSHKFYRHLDDNSTIAESNIGIRDDIYFYELDHAPSNWPPPKKKGSRYKSYSLLHGSSDEEIPDNASPLHDRVLIPVFHRGPSMSSYKSQNHAMALWPFHIVLTREEAKDYDTILRKVLAKVAQSTTRPILSELTGPSLGSRSGSDVVLTTEEDASPNDPQVKDGSIEGEDLVEVTMTDSATMSDVQTTAAKENGIPQVLLSGNDIGEALRALFEMKHTRKSNEIVTTGWSTVDHARALDSISKRIRVPSREESVQSSPAGSEGSSSDEDEDTTEPVDAKANIEAVNVSSDEEMTTFEPEESALSRGGRQGKKKNKKQLKRERKLERKHRNNKNFKSKKNRVPEQPNYPEDPDDETDESLIRMGEALVLEWSPQAYEALFGGTTADDPRGMDAMKFIETIEDPEIQEKKAKRVARRKHGIILDECFTESSKSEVLSEDNAWYCNRCKELRRATKTLDIWTVPDILIIHLKRFSGHRSFRDKIEELVDFPVEGLDLSGRVGYPESKNLTYDLFAVDNHYGGLGGGHYTATAQNFFDKQWYDYNDSIVSRCDSGQKAVTRAAYLLFYRRRSPVPLGPPELQKIVAADSRPDSEDDADDDEQRSRSPAGNGLRLGDSSRNGSSSAGAVGAGVGALRGGGSQLSAVASRPRNGAAAANHVTNDADDDDELDTLPPAYGGAASEDEGFHDASEADYVSQMYAPLNNYTDDAVWNFNALDRGEDRDPDDAASDAPNLGSVNGNSDDLSSRMLEDFGDEIGSAPRVSTPTDRLQVTLGDHGDDDDSDGEVAEIRLRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZV7</accession>
    <name>A0A9W8XZV7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amidase signature enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_010579</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZV7"/>
    <dbReference type="OrthoDB" id="5423360at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058329">
        <property type="entry name" value="Arp1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46310">
        <property type="entry name" value="AMIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46310:SF7">
        <property type="entry name" value="AMIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26053">
        <property type="entry name" value="DUF8016"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040952650" description="Amidase signature enzyme" evidence="1">
        <location>
            <begin position="17"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="Scytalone dehydratase-like protein Arp1 N-terminal" evidence="3">
        <location>
            <begin position="56"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Amidase" evidence="2">
        <location>
            <begin position="226"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4362485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="548" mass="59919" checksum="763D127CE9995F27" modified="2023-11-08" version="1">MFLPLVFSLFACATLATTSVKVFLQNYDGSLAYTLGNSSAVYYTPSTKSAKPVGVYNVKSKKDTLIPFTVVVVNETTITADVLKEVIKSYEVDDVWTPDFLGGIQLITTTQSAVLDVSGISYLHSLSKSDVIIDSIISCPTHNISTFLKSTITPPGPYLAKLTSGTISLLKTYATFYDQYDGFMNGVTANDDGSFAYARLWHPEQFEKIIPYPSRLYTSLLDPTIYPLAGLRFALKDIIDLAGILTGGGSREYPRLYNTPKNATAPAIQRLISLGAVPLGKAKSATFAYGAWPDQNDDIPYPWNPRADGYLGLSSSSIGSASAIGSYEELDFVLGTDTLGSVRNPADRVGVYGLRPTWGTIEVEGIITSAKTLDAVGFLTRDPLLAHKIQAVWDGANNPALTPGNFSYPKKIIYPTEWFPVNSSVAQNLVDAWLANMTAALGMQLEYQNTSQIFEEVVGYNGTLGDWTTNVSSLTIKDNWDTLGRQFVEDYGAAFGGRYPELDVSVREPWAEAPSYTQEEYAANVNRSWEFTEFWNEVGGPSHTNMHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y081</accession>
    <name>A0A9W8Y081_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009901</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y081"/>
    <dbReference type="OrthoDB" id="5430532at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="375"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="456"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="521"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="566"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="626" mass="69846" checksum="9D745E26E3B2E045" modified="2023-11-08" version="1">MKRKFSFNLAPVVSASTHFSEALPSLTSVQKLSGKSDTAEKEAPEPTPSHSQSSSQSHRRHNSKDTICDYSAYINRRVLDEDTEQELRIACRLILQNFKPSDHGMENTDPKLDFGGLERRKDPRENRQHREHKSQTADVIVRMPTGAPAELTSNFVARKPSTRTKTRTRDKADMEIRQRTQGDLPARANSSRKRADFGWLDDRDDKREQNLRKFGKSPSMDLPRPAALNVDGDSGVSTPVTVASTLAYSKQPSTAPTSASLPSGRSSNRRSRQLENPAAVADAQAAEWMRQELDKRRKEEASEPQARPSTAVRPPSRTASIKNNIKEYVFPGTRSRNLSRAQSTESLRTTDSQDPSQDPQRSGSAMGWRSWALPRRSGSRSNSRPGTSRGQSEEPEEKKKQDPKVVNLNRDLPPLPSLDTWKDPEPEPSKEDVAKSPRSPTSAAHIASLMRQQEPTQERSPARGREQHRRSRSDTLAVQYANAYPVRVSSHNKDVLQAAPQPTRATPVPESAIDTSRLEPGSSTHARTRSGDSLLSPTSPNGQSSLEVPSSFSRTISVDASSHSMTSREAKTSKREEQKSRLKKVFTGWMHKKDKKDDWMHRIEKEGVKEGVIVQDGSHVAPVVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0E6</accession>
    <name>A0A9W8Y0E6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0E6"/>
    <dbReference type="OrthoDB" id="40579at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901">
        <property type="entry name" value="2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901:SF38">
        <property type="entry name" value="PSEUDOURIDINE-5'-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="201" mass="22340" checksum="2A9DDC6E9991A9F0" modified="2023-11-08" version="1">MVSSFPPIRACIFDVDGTLINTEDIYSDIYNTILQEYGKPNYPWKIKAIQQSRGTSIFGDDPEMLHCDKKPSPDIFLLALKRLNNACVVAHDGEPEGLKPEECLVFEDSIAGVEAARRAGMRVVWVLHPGLAQVCRGREMDVLMGRTEEGGQAPDFSETEIDSSGSTVERNDCDGLMSEDGWAEMRLSLEDFPYGKYGINV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0H6</accession>
    <name>A0A9W8Y0H6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA mismatch repair protein PMS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PMS1</name>
        <name evidence="7" type="ORF">N0V83_009119</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0H6"/>
    <dbReference type="OrthoDB" id="10263226at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032389">
        <property type="term" value="C:MutLalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000710">
        <property type="term" value="P:meiotic mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16926">
        <property type="entry name" value="HATPase_MutL-MLH-PMS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03484">
        <property type="entry name" value="MutL_Trans_hPMS_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.100:FF:000001">
        <property type="entry name" value="Mismatch repair endonuclease pms1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000014">
        <property type="entry name" value="Mismatch repair endonuclease pms1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000120">
        <property type="entry name" value="Mismatch repair endonuclease PMS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.20">
        <property type="entry name" value="MutL, C-terminal domain, dimerisation subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.100">
        <property type="entry name" value="MutL, C-terminal domain, regulatory subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="MutL/Mlh/PMS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014790">
        <property type="entry name" value="MutL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042120">
        <property type="entry name" value="MutL_C_dimsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042121">
        <property type="entry name" value="MutL_C_regsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037198">
        <property type="entry name" value="MutL_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF52">
        <property type="entry name" value="MISMATCH REPAIR ENDONUCLEASE PMS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08676">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00853">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118116">
        <property type="entry name" value="DNA mismatch repair protein MutL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein S5" evidence="6">
        <location>
            <begin position="217"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="MutL C-terminal dimerisation" evidence="5">
        <location>
            <begin position="862"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="367"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="476"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="605"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="400"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="414"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="480"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="605"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="638"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="658"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00070941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4364524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1098" mass="120801" checksum="700C717F808DCD27" modified="2023-11-08" version="1">MATIKPIEGRSVHQIQSGQVIVDLQSVCKELVENSIDAGATSIEVRFKNNGLDAIEVQDNGSGIAPDDYETIALKHYTSKLATYDDLSSLQTFGFRGEALSSLSALSNFHIVTARAADGPKGTKLEFEQSGKLKGTSVVAAKQGTTVAVETLFYNLPVRRKELEKNIKREYSKVLQLLNAYACISVGIKFSVSNQVPKGKKTIAFSTNANPTTRDNISNVYGAKTIGALIPLSLEFELDPSNRPGATQNARNWSTQEDSGSHIVKIVGHISRPVVGEGRQTPDRQMFFVNSRPCNLPQVAKAFNEAYKSFNFTQSPFVFADIKLDTNAYDVNVSPDKRMIMLHDQTALLERLKESLLELFEGHDQSVPQAQLPGQKGVMSSFKPPLLRPLDSTSPAVLEHSSDAEDRNGEGRQPSRTIDLSSGTLGEFPRTGFIKASLIERFAGRDTEDRAIRSATGPRSNNLREVDKRIEELEARPTGQHRSASPGHNNIDQVPQQGSQSPLFEPEQAESQVPIRLSQPSRAVQDFNARIAEQHAQQLERDPSPAPLEHSAVQDQEEGRIPAITQTPQKALSQSTIQSAFDRMRPVRTPVQQATITVGDTTIISTVGSGSQSASSKRSRIHTPKFSLSGKPLNQTPKKSLFKTSLSSFAAPGTQVDSSDEGGSEDEASQNRSFQDAPARSPAPPTRPSRKAFEEPQTDDLAESSSPPPLPLPVIEVDSANEDLASATENESPGEAINAEELDDEYIDDTEKKVREEARVAQMIAEAEEAAARPTELNLKRASKMFKVTQKKFWTINLERVIETDAHRITQHLQSIQSSIDASIAAFEEAALLPSSTQLNKEDPEERLSLTVTKPDFNEMRIIGQFNLGFIIAVRPPTTTSPTSDLFIIDQHASDEKYNFERFSATTTLIPQRLVHPHPLELTAVEEEIILANEHALTANGFVIELDTSDDNNDASHHRAKLTSLPMSKEVTFTPKDLEELLALILDNPPSSSTSTSPHIPRPSKVRKLLASRACRSSVMIGKTLKHTRMREIVRHMGSMDKPWSCPHGRPTMRHLYGLEKWQGWSEGDGVIGVDNVQGETADWAGFLRKNKRQQGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0S3</accession>
    <name>A0A9W8Y0S3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009530</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0S3"/>
    <dbReference type="OrthoDB" id="37659at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44229">
        <property type="entry name" value="15-HYDROXYPROSTAGLANDIN DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44229:SF4">
        <property type="entry name" value="15-HYDROXYPROSTAGLANDIN DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="321" mass="35589" checksum="5B571BF3A3A400FA" modified="2023-11-08" version="1">MLDLCKNCTIRTNPSTNGTTMTKKVAFITGGVSGMGLKVAEALASRTNEDWDLHLVDLNAEAGKKVTASLKNSYFHQTNATDYQSLVSAFEATFNTTSRIDFVYANAGIVERDNFYEKHDLDAGPPPQPNQLSVDINFKAVINTSYLALYYFRKTIAKHGKDKVDPVLVMTASCGGLYPSEFCPMYSGSKAAVVQFQRAISYAYHQEGIRTFATCPGTIKTGLMTAEEWKSFPDEYYTPVETLVEAVTTIVDGGDVEDGKGRKVKAEDNWGLTVEVSGESFYFRDQVEFSDKRMEDMMKFTSMENQLARIEKTKKAQANGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0S8</accession>
    <name>A0A9W8Y0S8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_008603</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4364987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0S8"/>
    <dbReference type="OrthoDB" id="415706at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001425">
        <property type="entry name" value="Dynamin GTPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF149">
        <property type="entry name" value="GTPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G11890)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="5">
        <location>
            <begin position="26"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="4">
        <location>
            <begin position="596"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="705"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="705"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="744"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4364987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="793" mass="88700" checksum="BB42D8F9B0553CA9" modified="2023-11-08" version="1">MSLQSIETKNLLDIIDKLRSHGISRYINLPEIVVCGEQSSGKSSVLEAVSGVRFPSKDNLCTRFATELILRRGPDAPIKIGIVPGSQEKDRSEDDLEKLRNFHVSVSAENLQLEEVIESAKEAMGINESSRVFSSDILRLELSGPDQPHLTLVDLPGLFQAGSRSQSDADSETVKSLVLSYMKNPRSIILAVVSAKNDFNNQSITKHSRQIDPKGERTLGLITKPDTLDEGSDSERFYLELAQNKDVKFRLGWHVLRNRDYISRNTSVEERDKAEAVFFNTGIWRSLPANQVGVHTLKPRLSKILKDHIVAQLPDVLTQIKSGVRGCTERLDTLGASRATAQEQRRYLLQVSQSFSTLVRAAIDGQYSDPFFGDASTTEGYQKRLRAVLQNTLTDFATDMRKKGHTYTIVDEGPTKLPHHISRSDYVAKVTKLLKRSRGRELPGTFDPLIIEQLFHEQRKPWAALFDQYLEMILHAVSFLARTALAHVCDESTHAGLSRRFIYIRLEKLTAELRSKVAELLKPHESGHPITYNHYLTDNVQKAQSKRRAQEVKKSFEGVLGGDYTAVGVVNIPVNVNMLINTLVSKTEANMTSYASSTATDFMEAFYKVAMKKIIDDFSVLAVEACLIQELPLLFSPADIIDIDDATVASLASESEESSTERTLCSEKLKVLEDGLRALQNAREISSVPQDFDDALMQELEWKEHATETADEPDQRELVASQEEPLAAQKEPLAAQEEPLTAQEEASSAEINWTYPDQTPTPNDPLDLISPTPKRDLKKKKKWVKATVSELSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1U5</accession>
    <name>A0A9W8Y1U5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008647</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1U5"/>
    <dbReference type="OrthoDB" id="1933717at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322">
        <property type="entry name" value="PKSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322:SF736">
        <property type="entry name" value="RETINOL DEHYDROGENASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="312" mass="33605" checksum="E196F8A338F678E0" modified="2023-11-08" version="1">MDPAQFGLSTLSLQHAPYGPISVEKLQGANAGKVALITGAGQGIGAAISESLAKSGANVAILDLKLGNLENTKKACLSQNVKVEAFECDVTDDAKVKEVFDQVEKQLGPIDVLVNNAGILDQRPFIMSTFPAFWRQIEVNFKAPLMLIHNILPKMRSRGHGCIINIASRSGTVDVPMTLGYVTSKAALIRATHTLQKEMKLDGMDPAIHMYALHPGGVLTGMGGAGAAPDVRSKYGDITDEEFYKDLFKDAPVLCGQTCAFLASGKGKELRGLFIDCRQDVGKLLDIGRETLLKENRNTLTVNFIEGYTNEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y205</accession>
    <name>A0A9W8Y205_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Target of rapamycin complex subunit LST8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LST8</name>
        <name evidence="6" type="ORF">N0V83_008727</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat LST8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y205"/>
    <dbReference type="OrthoDB" id="400at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098588">
        <property type="term" value="C:bounding membrane of organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000339">
        <property type="entry name" value="WD-repeat protein pop3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037588">
        <property type="entry name" value="MLST8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842">
        <property type="entry name" value="G BETA-LIKE PROTEIN GBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842:SF0">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX SUBUNIT LST8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="71"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="112"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="203"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="267"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4365109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="328" mass="35989" checksum="1B2538F756DB76B7" modified="2023-11-08" version="1">MSVILCTAGYDHTIRFWEALSGICSRTIPHPDSQVNRLCISPDKRYLAAAGHHTVKLYDIKSTNPNAILTFDGHTSNITGVAFHCESKWLVTSSEDGTVKIWDTRSGNVQRNYTHGVPVNDVVIHPNQGELVSCDRGGNVRIWDLGENKCSHQLIPEDDVSVASVTVASDGSMVCAGNNAGNVYVWRMIQRSDTTSILPICKFLAHGTYITRVLLSPDVRHLATCSADHTARIWSVDTSAPHNITQADNLPSASERDPAAFPLETTLHGHQRWVWDCAFSADSAYLVTACSDHYARLWELGSQSIIRQYNGHHRGAVCVALNDTAVGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2J6</accession>
    <name>A0A9W8Y2J6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YCII-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2J6"/>
    <dbReference type="OrthoDB" id="5519740at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1060">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051807">
        <property type="entry name" value="Sec-metab_biosynth-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005545">
        <property type="entry name" value="YCII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33606">
        <property type="entry name" value="PROTEIN YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33606:SF3">
        <property type="entry name" value="PROTEIN YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03795">
        <property type="entry name" value="YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YCII-related" evidence="1">
        <location>
            <begin position="12"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="114" mass="12721" checksum="252DBF17BD552BCB" modified="2023-11-08" version="1">MASASSTPLQEWLVIVPDFEGALEKRIAVRSKHLEGLKSDAEDFWLWGGAMLEEPIQSGDTNPPKMKGSAMLIGAKTREEVVERVKKDVYVQNGVWDVSKIQIIPFKSALRKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2M4</accession>
    <name>A0A9W8Y2M4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Kelch repeat-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">KEL3</name>
        <name evidence="3" type="ORF">N0V83_008228</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2M4"/>
    <dbReference type="OrthoDB" id="4447at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025183">
        <property type="entry name" value="DUF4110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052588">
        <property type="entry name" value="Kelch_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46063">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46063:SF1">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13422">
        <property type="entry name" value="DUF4110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4110" evidence="2">
        <location>
            <begin position="595"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="445"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="528"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="276"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="463"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="559"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="691" mass="77338" checksum="1D6B3A354F0030F6" modified="2023-11-08" version="1">MAKDKKKATAGNKAAVKAAKAAKQEKKAGKKDKKVATKTQEVDSDAEDVDLDAVLAQYAKEQEQYHAITEVASEPPSARTSGTLIASPANENEIFLFGGEYFNGATAKFFNDLLIYNVKQDSWKKVTSPNSPLPRSGHAWCRAANTKDIYLFGGEFSSPKQGTFYHYNDFWKLDPTNREWTRVEGKSKTAAPPARSGHRMVSFKQYIILFGGFQDTSATTKYLNDLWIYDCVNFAWHTPKLQAARAVPDARSSFTLLPHEQGAVIYGGYSRVKAAAGGKQQQQQQKGKKGGGGGGPASRMVLKPKVHDDTWFLRITPPPADQPSTNLPTVSWEKRKKPANAPNPPRAGATMAYHKGRGVLFGGVHDVEESEEGIDSEFFNQLFIWNIERNRYMPLSLRRPKTNANKKAAQQANINRRDRGKAAEEELLANLKALEMKAGVAMADISDDEEEKAKEAEEEDEQRPEKPRIYEYPHPRFNAALTVQADTLYIYGGTFEKGDREYTFDELWSVNLNHLDGVTEIFKRELEDWQGSDDEADSDEEEEDEDSDEEDEEDENEKSTAASTAPTSVTDSPLVAPSKAVPDEDLGNQEEISALTDTLPNPRPFESLREFYARTSEQWQNLILDELTQRSKAIEKSPKEIKKMAFERAEEKWWDCREEIRALEDEQEAAGIGEVVSLADKEGAGGVGRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2Q0</accession>
    <name>A0A9W8Y2Q0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">UNC-50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_008474</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the unc-50 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2Q0"/>
    <dbReference type="OrthoDB" id="10027013at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007881">
        <property type="entry name" value="UNC-50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12841">
        <property type="entry name" value="PROTEIN UNC-50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12841:SF6">
        <property type="entry name" value="PROTEIN UNC-50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05216">
        <property type="entry name" value="UNC-50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4365853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="278" mass="31972" checksum="BB205F5AA32A95AB" modified="2023-11-08" version="1">MNITLPRPTGGPSNFGATTPSRRNEARMPRFFKRLFKFPQMDFEMAVWEIMSLIIAPKKVFRQIYYHKQTTKTYHRPDPSFTYLLSFLLTLTSLAWGFAYANGFTQTLHITLVFIFGHFLLLSLLTATLFFFLVGRLLGPGNSLLPGRRRGLYNLGEVEGGAGKEELEFGYCWDVAIRAFVPVWVFLYVVQFLCMPLVGTDHWVSILLSNTLYLFALNYYFIITFLGYNALPFLHHTELLLVPIAVTTILWFASLFGFNMSRHFAPVLWAGAKLRKHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y324</accession>
    <name>A0A9W8Y324_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Galactan 1,3-beta-galactosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_007990</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y324"/>
    <dbReference type="OrthoDB" id="3426327at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18821">
        <property type="entry name" value="GH43_Pc3Gal43A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006710">
        <property type="entry name" value="Glyco_hydro_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22925">
        <property type="entry name" value="GLYCOSYL HYDROLASE 43 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22925:SF39">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_5G14190)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04616">
        <property type="entry name" value="Glyco_hydro_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040940187" description="Galactan 1,3-beta-galactosidase" evidence="5">
        <location>
            <begin position="20"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4366354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="486" mass="54004" checksum="7407E652901A24E5" modified="2023-11-08" version="1">MGFLTLSSQLLLAATSVSAKWIIPGARWRDTAGNLVNAHAGSVTVDQGTGKFFLFGEYKVEGQVEGGGVSVYSSDDLATWEHHGQALEPIEGHPYISKEDIIQRPKVVYSKGTGKYHMWWHADNSTYGELLQGFAQADNITGPYSFVDATAPLGNWSQDFGLFVDYKDGRAYALYSNGDRKEGRDVYLTSYNENITALDKVVHRFDKYDLEAPTIVQTDKSYFALMSHKTGYRPNNVVAFRADSLSGPWSQPFIVAPLNTRTFNSQSGFTLRINGSAKTTYLYLGDQWDSISLWESRYIWLPISIDESRKSLEVQWHDVYDLDVKTGVVTPIKGKSYYAKDATVSGDAFLQEASFASHGVIATGIYGNDSKVTFSGIQGSGKPQWVSFYYQNTDDMGFGDQPGGTPDRINGTWQLRRISSVVVNGKVEEMETLYQRDTHKGIVLSTPLLLRLEKGGNNTVEIGGLYNGQDFKGADVDRIVVYEPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-06-18" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3P5</accession>
    <name>A0A9W8Y3P5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_007958</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112:SF16">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="738"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="1528"/>
            <end position="1566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="435"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="527"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="635"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1320"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1396"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1637"/>
            <end position="1674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="435"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="473"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="529"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="636"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1325"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1349"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1403"/>
            <end position="1433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4366322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1674" mass="183656" checksum="2C03460CE6DB61D6" modified="2023-11-08" version="1">MPQHQQQSLLKPAEDNTSTGFFFGDEGIDTTTGFSQQFDTMNPALLSTPQTSFDMQHTLVRACVEPVSSLRTDSDQAMDNTVGSATAWNGQLTPNSHHALNTFPPTPIQSFDSLYQPNFSTALGKRPLQLDAQDFPHAKRHETADFTLFSDLPSTTTTSSWGLETQSTPRSIEVGLSDEAADVCATWFNKYAVLPSDRHIDSLSQLTGESADAIRSWFGRLLKQGMGGSHGDSAYKSQTNLNIQQDTFWNDHFSTGVAQTSPPQLLLQQSQEDTTSPEAACSHENTTVVQPASTLRGSKKRCTPTEDRQLLSRDPNKIYQCTRKCGKRYGRKCDWKRQEEEAYPCKSWVCSLCTSEGVENVKPCFRKYHFVQHFRNIHPEINPAEYEEASVVSSETEFPRKCGFCRHRFVSRQERIDHIADHFKHGKCMLDWKEEADDDSGSDNTDDDDDRPSGDGFDGSAPSYQPPHSDPRGGAGSKYNGGGSDSSGSDDQPPHGGFFQFQLSQLHEGGSGSQPSCAEQHIKPADALQSDQQQRSSFRSSSGEQSSTGGSISSPFSKHVPAAGDNKDTLAGDALSRSLIDQSPRAATDATWLLEKPAFEQWRNASTSKTLWLHDQPGAGKSVLSAHIINHLQELGHNSSQDSSNDSKTITLEKPTSTNRTRLQILDDYRSPSPEVLQSTSATPAQKVPSTGVQSSQFASVLLESESKVTSPTHNMVIGESLIAQQALQSISPTSQSFLSVRLLGAGGFSTVDEVVHRDTNLRMGRKTLKNRDQTAIEEIRKEVNVLQKLRHPHVIRFLGAYSTGNKMSILLSPVADTTLALWLEQSSQEKPPGLTETVVKMFGCLASSVRYLHESRVRHMDIKPQNILVVTGDQEIPHVVLCDFGISSSSDVLDGQTKPLTRQYTAPEVFEGFTRKRAADIWSLGCVFAEMASVAFSQGNPPWLNFRKEFSGRTGKYYWQDVPALQGRLSAFLEDSSTSTEQTVLRTLKSMLSAEPSERPDGTALTMVFTPAPCCLSWPNDKATFPGPHEESGEVEMLICEDSVDCYTHSHVRGTTQETDIDFSTARGWLEECSHSHDACRQQALSGANVLPTRLVDVRPNRQETSYVRLVNSASLDSTTGKIDYVALSHVWGQDDVALSSSSLQDMLTDLPLQALPTAVNAAISTAQRLGYRYIWVDSLCVLQDSEDDKRRECAAMASVFRNAALTVVLDQMTTKLAGEDKPSVPSTSDKLDQALLLTPETRRVSTEDRLPASASLPAIDFATPGFAWDTRAWALQERLLSRRFLHLGEQMYWECNSLKASETFPRGLSPLVWEKVHSKNTEGRQQPRVSSRTTPTDTAALPSSHTHCADKSGGLDKKLRSVTPHHLRNCQWIKKEGDCIGDSMEAAQTTLKFGGRDAEASSSSLSGLQRGRTTRTPDQQRTPISTSSPRSRTLLNEHEIHHCDVHESAAQHASFVVDAAKTHGRHGDLKPANILWFQRESNESEDGPFDTLKLERARERVQSGIATEHHTHCTSTAVDGHSSPRFYCPTACTAAFTPKANLKRHEQSGIVPIDDDNTHMHDSPANAADDGLTTTTITTASSSSLVAAAANVKGIGGRKVGRKDISLSGQLDQHGMSHGYAENIITMEDRDACVKERSGNGDADEESSAGSAAGNIDTGSDGQLVVMKDRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y410</accession>
    <name>A0A9W8Y410_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">SLN1</name>
        <name evidence="20" type="ORF">N0V83_007902</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y410"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007234">
        <property type="term" value="P:osmosensory signaling via phosphorelay pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000004">
        <property type="entry name" value="Ethylene receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000289">
        <property type="entry name" value="Osmosensing histidine protein kinase SLN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047:SF72">
        <property type="entry name" value="OSMOSENSING HISTIDINE PROTEIN KINASE SLN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="18">
        <location>
            <begin position="552"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="19">
        <location>
            <begin position="985"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="724"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="877"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1109"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="511"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="727"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="877"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="896"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1123"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="14">
        <location>
            <position position="1040"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ4366266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1172" mass="129090" checksum="FF3995082798DF1D" modified="2023-11-08" version="1">MVKMRIPIREQLGCLVLLASMIGLAVIAIATWVTNHNFVLDIRSTRLTLTASLKSAQLSSNLALMQILVKQATNRLAPQTALDHYYQGNTSDANWVRTQEDYDAVFSGDDKTRIALQVQIYPYYSSDESIFNRTAYAMQDVALPYKTSSGENATLGDPTYGFIPELYPKFAVRTTPYNSTFNTYQAEFNGREIDGSSYLLSGPYRVNDSLSLVSITMPIINNTSNIDTLGWLTVVLDASLITDVVEAMEGLDNTGVTMLFGPNNDTNNFPTGYLYNSPNPDPPKNADVRFLYPPTFRSNVMRHGQYDTALDPPPFDWANFVAIRRGFTEATGATNNAGSMISTRNEEGESVAVGYAVVNSAMVNWMVVVEQTHGEVWGPIYRLRKVILACVFGTMGAMLLLTFPVAHYSSRPIRRLRDATKKTVSPHMFEDDSISSQNDGTNEDPDDLALAHKEGFFGSIIHYRRNQKLSRAEKREAERRRQFRIPSKVKDRKHFIHDELTDLTKTFNEMTDELMMQYEKLEERVAQRTAELEQSKKAAEAANESKTLFIANISHELKTPLNGILGMCAVCMSEDDPTKLRRSLGIIYKSGDLLLNLLTDLLTFSKNQVGQQLNLDEKEFRLRDISQQILAIFDKQAKEGGINLTVQFEGPYENNLDDNGRPNYFGDLGPFGLGRLKDMILYGDQHRILQVVINLVSNSLKFTPQGGAVTLTIRCVGEASMSDSRKASLQSRQSSMRNSKTRFRATSSEVGSISVATPTNYDTANVINAMDKPNTYSQMMAHERTATPPPGRWLSFEFEVEDTGPGIPEHLHAKIFEPFVQGDLGLSKKFGGTGLGLSICSQLAGLMKGTIGLKSEVGHGSVFIMAIPLKHLSSRADSTASSNNINLDTNTPRRSLSMEETRGRTHDRTEDALSTRSVQSATSGPVAGTAAAAGPVAFETDSKPRLVGLSQPFFASTAPLESPNSQAAAMQRVEAEATKRGGKVKVLVAEDNKTNQEVVLRMLKLEDVYDVTVAKDGQEALDKVKESMDNQTPYNLIFMDVQMPNLDGLQSTRLIRQSGFSSPIVALTAYAEESNVKECLDSGMDFFLSKPIRRPALKHVLKTYCPTIPEEEPEASTPPADSSKPNGKTTGPATILGNTNAATAPVTVIAGTSDRRHPDDSPAISPMTSPQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y417</accession>
    <name>A0A9W8Y417_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 18">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="6 18">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">PGK1</name>
        <name evidence="20" type="ORF">N0V83_006890</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines. Negatively regulates the biosynthesis of acetyl-CoA from pyruvate in the mitochondrion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 19">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="6 18"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y417"/>
    <dbReference type="OrthoDB" id="275353at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00318">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000003">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000019">
        <property type="entry name" value="Phosphoglycerate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="entry name" value="Phosphoglycerate kinase, N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406:SF0">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="11 18 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18 20" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="24"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="64"/>
            <end position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="373"/>
            <end position="376"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00016471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ4367309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="417" mass="44718" checksum="A1118CFCD6C8E107" modified="2023-11-08" version="1">MSLSNKLSIADVDLKGKRVLIRVDFNVPLDSDKKITNNQRIVGALPTIKYAIDNGAKAVILMSHLGRPDGKPNPKYSLKPVVPELEKLLGKSVTFTEDSVGKQVEDTVNGASNGQVILLENLRFHAEEEGSYKDEAGKKQKVDKAKVEEFRKGLTALGDVYINDAFGTAHRAHSSMVGVDLPQKASGFLVKKELDYFAKALEEPKRPFLAILGGAKVSDKIQLIDNLLGKVDSLIICGGMSFTFKKTLEGVKIGNSLFDEAGSKTVKDLVEKAKKNNVKIVLPVDYITADKFDKDAQVGYATDEEGIPDGWMGLDCGEKSIKLYKDAISEAQTILWNGPAGVFEFDKFAKGTKETLDAAVDAAQSGKIVIIGGGDTATVAAKYGVEDKLSHVSTGGGASLELLEGKDLPGVSALSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4H5</accession>
    <name>A0A9W8Y4H5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_007927</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4H5"/>
    <dbReference type="OrthoDB" id="20872at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39608">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G08640)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39608:SF2">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="7">
        <location>
            <begin position="55"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="207"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="219"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4366291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="233" mass="25350" checksum="F5D01B46EEE8999A" modified="2023-11-08" version="1">MVFGRSRAARGDNVVEHGVGAPATGGVGHHRNTDYGAPVDRSDKMHRPLLLANHILHWISSLIVMSIAAYFISKYHSRRNTHLIYWVTIGALDAILYLAFLVLPVMKSYKGYGAPLPWIFSYLWLTAFIFATQDYSDGRCVYNSPTFVTECGLKKTIAAFAFIAFFTNLVGTLLEGRLWDAHRVRGGAHHTGGTAFDNDKHGHHGGVATGGRNVVGEPTTTTGTMGTTGTATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4R6</accession>
    <name>A0A9W8Y4R6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4360 domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4R6"/>
    <dbReference type="OrthoDB" id="152248at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025649">
        <property type="entry name" value="DUF4360"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38847">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38847:SF1">
        <property type="entry name" value="PSEUDOURIDINE SYNTHASE RSUA_RLUA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14273">
        <property type="entry name" value="DUF4360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040907395" description="DUF4360 domain containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="21798" checksum="7010F35FA35951EE" modified="2023-11-08" version="1">MKYTFTALALAALSLASPVPDTTSAPLTFKITSVASGGSGCPQGSIDINWTDNRILPIVFNKDFTAKVGSKTDVVESRKNCQINLGLEFTPGYSFAIFSADYAGWGDLDNGVTGIVKSTYYFSGEQDQSSTTLELDGPWHGQYYKSDKVALSYWSPCGPGDAMLNVNSEVALTPFSSALNGMLAAGREGARFSSNLNIQWRKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y511</accession>
    <name>A0A9W8Y511_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">galacturonan 1,4-alpha-galacturonidase</fullName>
            <ecNumber evidence="12">3.2.1.67</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Galacturan 1,4-alpha-galacturonidase B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Poly(1,4-alpha-D-galacturonide)galacturonohydrolase B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">N0V83_007243</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Specific in hydrolyzing the terminal glycosidic bond of polygalacturonic acid and oligogalacturonates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl](n) + H2O = alpha-D-galacturonate + [(1-&gt;4)-alpha-D-galacturonosyl](n-1)</text>
            <dbReference type="Rhea" id="RHEA:14117"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14572"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58658"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.2.1.67"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.67" evidence="12"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y511"/>
    <dbReference type="OrthoDB" id="187139at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047911">
        <property type="term" value="F:galacturan 1,4-alpha-galacturonidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000040">
        <property type="entry name" value="Probable exopolygalacturonase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31736">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31736:SF6">
        <property type="entry name" value="EXOPOLYGALACTURONASE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 16" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040787281" description="galacturonan 1,4-alpha-galacturonidase" evidence="17">
        <location>
            <begin position="20"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4367658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="456" mass="49873" checksum="78D13C864391F013" modified="2023-11-08" version="1">MKSLSLLLSLTASLALTSASVLDPIPAGASITLPRSVSGPSRFSLLGRGNHKKKKDNWGNAVEKDRKEVKIRASKNDQDDISDDFLWAIKKANHGGLVHLEKGKKYVIGKKLDLTFLKDVYVKIDGELKFTNDIEYWQKNNFYYDFQKSITFWVWGGKDIKIYGNGGVINGNGQAWWDGFSGAEILDPNNVFYRPILFLTDNATNIEITGLHLKDSPCWTTFLVRTKNVVFDDIYIDAVSTNVSSLPKNTDGFDSLNVDGLTVTNTRVNVGDDCFSPKPNTTNIFVKNLWCNGTHGVSMGSIGQYPGVKDYISNAWLENITLLNGQNGARLKAWAGPDVGYGYIDNITYKDIHIENTDAPVVLDQCYFNINETTCAAYPSKVNITNVNFLNVHGTSSGKNGRVVADLTCSPGATCSGIHLEGINITSPAGSPSQIVCDNIQGDIGVECISASEADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y527</accession>
    <name>A0A9W8Y527_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RNA helicase</fullName>
            <ecNumber evidence="4">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SUV3</name>
        <name evidence="15" type="ORF">N0V83_006550</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y527"/>
    <dbReference type="OrthoDB" id="6692397at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045025">
        <property type="term" value="C:mitochondrial degradosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000965">
        <property type="term" value="P:mitochondrial RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17913">
        <property type="entry name" value="DEXQc_Suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18805">
        <property type="entry name" value="SF2_C_suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000269">
        <property type="entry name" value="ATP-dependent RNA helicase SUPV3L1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.40:FF:000002">
        <property type="entry name" value="ATP-dependent RNA helicase SUV3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000957">
        <property type="entry name" value="ATP-dependent RNA helicase SUV3L, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055206">
        <property type="entry name" value="DEXQc_SUV3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022192">
        <property type="entry name" value="SUV3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041082">
        <property type="entry name" value="Suv3_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044774">
        <property type="entry name" value="Suv3_DEXQc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE SUPV3L1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22527">
        <property type="entry name" value="DEXQc_Suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12513">
        <property type="entry name" value="SUV3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18147">
        <property type="entry name" value="Suv3_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="209"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="327"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="622"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="567"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="328"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="630"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="642"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="664"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4368194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="689" mass="76815" checksum="53153542C8BF3BA1" modified="2023-11-08" version="1">MADFRALDKPMDQASDARAGRAGQRRFTGEEYAHDAPEIYVLRADMWWALHRGGTGESEGGPTNSGKTYHALKRLEEAETGIYLGPLRLLAHEVYTRLNAKGKPCGLVTGEEQRMPENEQARMYSCTVEMAPLNTLFDVAVIDEIQMINHPDRGWAWTQAFLGLQAKEIHLCGEARTVPLMRELCALVGDKVHVHEYQRLTPLQLQPRSLNGKLTHLEKGDCIVAFTVLGIHALRREIEKKTGKKCAIVYGSLPPETRAQQARLFNDPDNDYDFLVASDAIGMGLNLAIKRVIFESTIKSNGVNYVPLQISEIKQIAGRAGRFKTAHQAVSQDSAQDSVTGNAVDPAVLDGRNPKPKPEQKTIGWVTTLDQVDHNNLRLGMGREPEEIKTAGLFPPSLIVERFASYFPPGTPFSYIMLRLHEISEIHPRFHLCALKDQLAIADNIHTIKNLSVQDRIMICSAPSNMRDTTERAFLRALAECIADGKSGDLLDLPDLPLNVMDHQPSGDRKYLYALEQLHKLLVCYLWLSYRFPNVFTTRALANYTKKLVEDQIEKTLTDFSFIEQARAKMRQRREKARKDLDKQDKQAVLLERMEGGGHREILAPPGLPLEAGDIVNDTTAFAEGAPLPDDAEEYPDEPEPAEAGGDDSAAAAEGRHQRGTRNLSHEVGHVLERQRRDAPDSSTSSSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5B0</accession>
    <name>A0A9W8Y5B0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006685</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5B0"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4368329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="236" mass="26014" checksum="AB11605EA3943CBA" modified="2023-11-08" version="1">MDHLKDEGEWVTFDEAGAFSLRSGFKDDIKEVWECEEYDPSQPSQISQLGEIGGRLGSEPGVVIDIYENCQLNKPWTLEVFKAGRGPGEDTEDFPSFLFLSAGQAREFSKDGRPRVTILVPAALDPQARPLMNLESTLEKFAAFETRNYHDMLEDVTDLYAKSGPAAQGVEKIRAAMGGGNGIINLLGLGILLPCSVPSFLVDAPGWDLLEAMRVRKDVDAATRKAVMDFYQSSDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5E2</accession>
    <name>A0A9W8Y5E2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_006731</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5E2"/>
    <dbReference type="OrthoDB" id="2151789at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF22">
        <property type="entry name" value="FAD-BINDING PCMH-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="5">
        <location>
            <begin position="39"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4368374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="473" mass="50996" checksum="0913ACDC2B6F45BF" modified="2023-11-08" version="1">MSVSEAIQALKAISASLVVLPGSDEYKEISRSYLSTVESDLEPAAFALPSTKDDVAAILKALKPFDNKVPIAICGGGQQPAAGVANVAGGITIHLRRFKGIELSQDKTFVSISPGETWGEVYKKLEPEGLAVSGSRAGTGGIGGLSTHGGISFFSSERGFIADDVVNYEIVLASGDVVGANAKEHSDLFKALKGGGGSNFGIITRFDFTTFKQGKMWGGKFMYFPASFPQQLEGIAEYLNGPNPDPRAQVRINIGYAAELGGMMCMNDPTYTKPEKPEGFSLFTDVEPRIPQVDTLRVATVSELANEQSEQSEYKKRVIYLTTTLKANAEVLKMTLEKFNKGFEPIKSVAGLIYAATYEPYPVSLLKASTGNSLGLDANDGPLVLFLLYTSWSDTKDDDAVIKANKEVLGDIKKEAEAMGQLSPYTYMNYSFPGQDPISSYGPEIKKQLQAVSQKYDPEGFFQKAVSGGFKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5L8</accession>
    <name>A0A9W8Y5L8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5L8"/>
    <dbReference type="OrthoDB" id="10250120at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF18">
        <property type="entry name" value="SORTING PROTEIN SNF7 FAMILY PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G16692)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25880">
        <property type="entry name" value="WHD_CHMP7_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="446"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="469"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="518"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="537" mass="60325" checksum="DE10B2357778A986" modified="2023-11-08" version="1">MLKRKLSHDFSSGPHVSIYGDDPTRMTSKRKQLFRPRNDNKQAWTPEKVLEMSELLEFVLQHEEAFKNQYRLASLYADFRQQLEINPEGYHANIAAWTKALTDASRAGVIPMQGTVRDLLNIRTADELARALQHPQYGKPTCLPAVFHEAVQNKEMIPLKDFLNAKESIYKTSWIPSPWKVLRWSLRQVGVLGELKSPDKLEVGNFVVVKNIEIAADEILKKMMEHVSTADRVLSRSDFLKRFSTVLNDAAPLTTNDLNILLVFLARDKHAISYDAKTIKFKPEHEAVPLPITEEDAAIANLRDTVANINAQLPPLMEKIAAADAAAREAVAAKQMIRAKAALRSKKLAESALAQRSDVALQLESVYTDLQHAADQVEIVEAMRAGAAALKGLNEKVGGAEGVQGVVDAVNEQMATTEEITNIINETGQPLDEAEIDDEFEALERAEREKQEREEAEKTAARLAELEEAENRRKEKAAEEKARKEQETERDAEKEDDKTEQKVVEASQGLSRLSFYEQEAEDEMKDAEEEKRVPLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5N2</accession>
    <name>A0A9W8Y5N2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major royal jelly protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_007093</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major royal jelly protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5N2"/>
    <dbReference type="OrthoDB" id="7776143at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017996">
        <property type="entry name" value="MRJP/yellow-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10009:SF17">
        <property type="entry name" value="MAJOR ROYAL JELLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10009">
        <property type="entry name" value="PROTEIN YELLOW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03022">
        <property type="entry name" value="MRJP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4367510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="414" mass="44993" checksum="F495DE0F6C3B4BA9" modified="2023-11-08" version="1">MLLVPSSSSAKYLDIPTNGSSGTGHGAYPQDFPILGPSLEAVHESQKPPTGLAVDVSHNIYLTYPRNFGSSQYNVVICTSFNDEQPWPNSAIQNCTTGQDPSTCFINVQNVVRDDLSRLWVVDSGIPYNAAPNLDAIYGGAKIMSFNETTAEHLRTYIIPQELLAHRTNLNDMRVNTTLGGREGYAFIPDASVNSSLLAIDLEDGSTVRRLFNTSVVRADPDYVGSYGGGLTYTWNGTTKGHLTTAADGIALASGNLYWGVLASRRFYYISQEVFVDTTKSDAEIVAAVQNPGQCGSEQAGFSADDQGRVYIAASEQNSIYYVDTLESQVNQTVNGNAPGGSGLVPAKDYVVKNLVRNAMIQHADSLAIMDGWLYFCTNQLELGPSFQYNNTDKRVGPFRSFKIWIGRGPARDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y612</accession>
    <name>A0A9W8Y612_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PH domain-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_007519</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DCP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y612"/>
    <dbReference type="OrthoDB" id="440673at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13182">
        <property type="entry name" value="EVH1-like_Dcp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010334">
        <property type="entry name" value="Dcp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290:SF0">
        <property type="entry name" value="DECAPPING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290">
        <property type="entry name" value="TRANSCRIPTION FACTOR SMIF DECAPPING ENZYME DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06058">
        <property type="entry name" value="DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4366989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="29897" checksum="C856A8ED50C8057D" modified="2023-11-08" version="1">MAPRAKARPSHPQPQPSDYETDAAPATVDVPLPPPRSNEELNFAVLSRVYPQLVAIEHVTPYAALYTFNLDAQQWEKVGVEGTLFVCQLAPSPIGVERYCVIIYNRRGLDNFYLDLTSSDDMEITDPYVILQGDEVVYGIWIFADPPPASTAHCRVETANKMMEIADRAKASREAKEREHKHDIREATEHVEAASSAPMGRQLSLRELFGQQREQDAGFSVHNHHSQLPLQQYPQQVYAMQNSGPAQQDVLGQLFMKAKQDYNGFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6Q2</accession>
    <name>A0A9W8Y6Q2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_006739</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6Q2"/>
    <dbReference type="OrthoDB" id="4132249at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050797">
        <property type="entry name" value="Carb_Metab_Trans_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668:SF18">
        <property type="entry name" value="MALTOSE FERMENTATION REGULATORY PROTEIN MAL13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="43"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4368382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="557" mass="61199" checksum="DE9AFAF42776F275" modified="2023-11-08" version="1">MSTAPRKGTRRACDPCAVRKIRCDGKTPCSRCEAASWECTYLKTHGKSGPKGPRRTTEAAIRRLQESSKSSHDESRSNSEASLDAGSPVTTDLPYHEQFPPIIEPNTEQQGWPDIIGPALPAGQREPKKISTSCISQYLDIYQTRGYGIWPVVDAEALTARLLTHPNDSEAYALATAICAAIVSQFSIDAEPGSPVEGHYRVSSAVFEGEAKRARKESDHMENVTLFSLLGSFFLHVYSANTGRMNVSTVLLGEAIVKAHILGLHKPSHYHAMTAEGIQHTLRVYWLLFITERAHSLQHDVPTTLRRASNLPSLENLNDGSVTPAFIQLCRLFNILDVTITADPATARNALALAQQQLSSDDGPLSLENELQRADITMTQQWMRIFLWQHALNVTNLSSADRSEEFSFSFPAKVAQNVLSNLSSLSRQSIEAHGPGMESKLFDVANSLADVMMIMPSMNHESHFGIGPRDLIHSLSQVLSSFRGGNPAVISILQHKLTTLGLAVNSPQKLLDISSPEEEHEEWHDRSLSMPSSYPAFANVAPISPSFPTSMSMDGDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y720</accession>
    <name>A0A9W8Y720_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_005966</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y720"/>
    <dbReference type="OrthoDB" id="5086884at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17325">
        <property type="entry name" value="MFS_MdtG_SLC18_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050930">
        <property type="entry name" value="MFS_Vesicular_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506:SF23">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="400" mass="42322" checksum="2C3950373FC4AEE1" modified="2023-11-08" version="1">MADFFGAPLCAWYVDRAQSRRLPWYFGIILITTGSLLFGLSSNIAMLMCSRVLHGVSSSILYTVGLAVLVDTIGKDEVGQWMGTAMSCNNVGIIISPLLGGILFDKGGKMAVFAIMLGLGAIDIILRLFMKEPPRETRVFMKSANSSGSFETGNGDEKEKTQISVVILPSTSESEPVSTSTAFRTRRLPGILALLRQPRLLAAMFGVFINETIIASLCATLPLFVHSTFHWTALAAGLIFLCIAIPTFGGPLAGALSDRFGARWIAVSGFALTAPMLVLLRLVQQDSLEHKVLLCALLTMAGAALTLFLAPLGAECSFVAEETSEEMGCDLYASSFSLMNCALASAGLLGPLAAGGLVNAAGWKWMTVALGSFCALGIVPCALVTGNRRAVQTSNDDSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7C6</accession>
    <name>A0A9W8Y7C6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome c oxidase subunit 12, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cytochrome c oxidase polypeptide VIb</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">COX12</name>
        <name evidence="8" type="ORF">N0V83_006180</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome c oxidase subunit 6B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7C6"/>
    <dbReference type="OrthoDB" id="1107506at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00926">
        <property type="entry name" value="Cyt_c_Oxidase_VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.140:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 6B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.140">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048280">
        <property type="entry name" value="COX6B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036549">
        <property type="entry name" value="CX6/COA6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003213">
        <property type="entry name" value="Cyt_c_oxidase_su6B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11387">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02297">
        <property type="entry name" value="COX6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47694">
        <property type="entry name" value="Cytochrome c oxidase subunit h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00074891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00082359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4369097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="140" mass="16349" checksum="9E635DBBA13F682B" modified="2023-11-08" version="1">MSDNENGDELVTKPFKFVTAAKALKNTWNMFMRSLGYATQGADELRRSLGFDARFPNMNQTKHCWQNYVDYHKCIIAKGEDFAPCRQFMLAYKSLCPSGWTTRWDDQRGEYPLFRFKFLDLRSISGADDASLKRCIKNEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7F9</accession>
    <name>A0A9W8Y7F9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Non-reducing polyketide synthase PKS8-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PKS81</name>
        <name evidence="12" type="ORF">N0V83_005518</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Non-reducing polyketide synthase; part of a gene cluster that mediates the biosynthesis of a yet unidentified natural product.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.366.10:FF:000017">
        <property type="entry name" value="Non-reducing polyketide synthase aptA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.366.10:FF:000002">
        <property type="entry name" value="Probable polyketide synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.10:FF:000011">
        <property type="entry name" value="Sterigmatocystin biosynthesis polyketide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.110:FF:000001">
        <property type="entry name" value="Sterigmatocystin biosynthesis polyketide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000031">
        <property type="entry name" value="Sterigmatocystin biosynthesis polyketide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030918">
        <property type="entry name" value="PT_fungal_PKS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032088">
        <property type="entry name" value="SAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04532">
        <property type="entry name" value="PT_fungal_PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF37">
        <property type="entry name" value="SI:DKEY-61P9.11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22621">
        <property type="entry name" value="CurL-like_PKS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16073">
        <property type="entry name" value="SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01294">
        <property type="entry name" value="PKS_PP_betabranch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="10">
        <location>
            <begin position="390"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="11">
        <location>
            <begin position="1320"/>
            <end position="1633"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="9">
        <location>
            <begin position="1724"/>
            <end position="1801"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="8">
        <location>
            <begin position="1320"/>
            <end position="1465"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="8">
        <location>
            <begin position="1484"/>
            <end position="1633"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="8">
        <location>
            <position position="1352"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="8">
        <location>
            <position position="1542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4369754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1802" mass="196132" checksum="83DD25801F7E2247" modified="2023-11-08" version="1">MSNLEVVYFGNELPKEDLQDVFRKLHLHSKDRRHPLLARFIAESTQAIREEVRRLPSDLKHIIPPFDTVLTWAEHNDLREGPLCGAIDGVLLTIVQIGTYIGYIEKNPKSLRNFSKTSVAGLGVGLLASTVVALSPMPADLPLAGADAIRLAFRMGIHVQEVSENLEARDLLGDPETWAFVVHNVDHAAAQKELDLIHTNENAPDTAKIFVSAISATSITISGPPTRLKGIFNKSRYFRDARSIALPVYGGLCHAPHIYNQKDVEGILDTRSASLHTVAAETEPVMGIYSTSTGQPYSAKDPEELYSSVLAELLTQAICWDLVVDGLVDAAKLAESSEVVLHCFGNSLSLKDLSTSLKSKISDIDVSVNNLATWVTQKLSENTAPRGTKQAKLAIVGMSCRLPGGATNTEKFWEILEKGLDVSRRIPADRFDIDTHYDPTGKQLNKTMTQYGCFIDEPGMFDAPFFNMSPREAQVVDPQMRLSLVTAFEALERAGYVGNRTASTQLQRIGTYYGQAADDYREVNQGQEVSTYYIPGGCRAFGPGRINYFFKFSGPSYSIDTACSSGLAAIEVACQALWNGDVDMAVTGGVNVLTNPDGFAGLCNGHFLTKGHNACKTWDATADGYCRADGVGSLVIKKLEDAEADNDNILGVILAAGTNHSAEAVSITHPHAGHQSYLSRQVLRQAGVDPLDVSYIELHGTGTQAGDYEEMQGIMDVYAPLTKRRSKDQPLYIGSVKSNVGHGESVAGTTALIKVLTMLQKNAIPPHIGIKTDINPRFPKDFDKRNLHIAFEKTPWIQVAGKKRIAAVNNFGAAGGNTTMVLEDAPRRADSEADPRKTQVIAVSAKTKGALSANIENLIAYLDKHEDTSLSDLSYTTTARKYQFSHRVAISASDMGSLKKQLVSRLAKVDSMKPVGKSGSPPIAFAFTGQGASYKSMSLELYHHAPTFRNEIDLLSSLAQAQGFSPFIAAVDGSHPQDYTHSPVVTQLALVAVEIALARYWMSLGVKPDVVIGHSLGEYAALHVAGVITASDALFMVGRRAQMLTEKCKIGSHTMVAIRASLKQIQEAAAGKPYTIACVNGPSDTVLSGTTEQMEDVVASIEAAGFRCIKLDVAFAFHSEQMDPILEGFEAACKSSVLFHEPKLPVISPLLGKAIFDGKTLNANYMRRASREAVDFLSALESAQQMSTVSEETIWVEIGPHPVCTGFIKAIMSKHQPIAIPSIRRGVDNWTTISESMATLHQAGVTLNWNDFHRPFETRLRLLDLPTYAWNEKNYWLQYNGDWCLTKGNNFYDTPEGSSTTALESSVPLVRSEVQTSTVQQITELSIDGAAGTVVMQSDLMQPDFLAAAHGHRMNNCGVVTSSIHADIAYTLGNYLYRKFYPNVKDINLNVADLVVTKGLVAQSSTKTPQLIRVTASTTDINSGIMDMTWQNVDSSYGSADEPFATASVFVEDASGWLSTWESMAHLVHGRIEALEVLAAEGKASKLSRSMAYTLFASNLVDYADKYRGMQTVIMHGLEACTDVQLTEKESGIWTVPPYFIDSVAHLAGFVMNCSDAMDTQNNYCVTPGWSSMRFAEPLVAGAQYKSYVKMIPSKEDPTVYFGDVYIMRRKDMQVIGMVGGIQFRRYPRILLNRFFSPPDKASAKESKVQKRDVPGSVVPAALPLKAARIPVTRDFPVNLPNARPGPIKALTPAPASTITAPEPILAPPPEPEPMPIVAAAASASDNSTTGKAISLIANEAGLELEDLTDDASFADLGVDSLMSLVISEKFRTEINVKVGGSLFLDYPTIGELRKWLDEYYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7K1</accession>
    <name>A0A9W8Y7K1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_006302</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7K1"/>
    <dbReference type="OrthoDB" id="6752799at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006532">
        <property type="term" value="P:aspartate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000066">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF55">
        <property type="entry name" value="GLUTAMATE OXALOACETATE TRANSAMINASE 1, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="28"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4369217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="410" mass="45665" checksum="89A6407E4E299A06" modified="2023-11-08" version="1">MFFQDVPQGPPDPVYILKNAVDADKCPEKVDLGVGVYRNERAQYNELGAIKEAKKILTQKELGHDYEVTTGDAEFVRNAAAVLFGKNNKAIKSSRITSVQAISGTGSIHLAALFLSRCASFKGKQVYIGTPAWGNYEPLFNLVGLKVVKYKYYDAAKGVVDFKSVLDAVSQAPEGSMFVLQGCCHNPSGADLKPEQWRELANVMRPRKLFPFFDMAYQGLGAGLEEDAYGLRYFEEQGFEFLACQSFSKNFGLYGERCGVLHVVSEGEKVPANVYDQLRCLIRWEFSSSPAYGARLVNTILGNEELSTKWTNELITMRQRLISLRRALYSELVEKRKTPGNWSTILSSTGLFCFLPLDPRQVSTLATQHHIYMLQSSRINVSGMNQSNIERIASSIDEVVRSSGTVLANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7W2</accession>
    <name>A0A9W8Y7W2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_005701</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7W2"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016652">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19120">
        <property type="entry name" value="AKR_AKR3C2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044494">
        <property type="entry name" value="AKR3C2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF3">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="7">
        <location>
            <begin position="25"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="6">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4369937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="297" mass="33187" checksum="B4BE65DED924C42B" modified="2023-11-08" version="1">MAKLDLTIPNLKLNDGNSIPMLGYGTGTAWYKTGEESKIDQAIIDAVKTATKLGYTHLDGAEMYKTETELGTAIKESGVPREKLFVTTKVDNGNIHDIQGAIKTSLKKLQLDYVDLYLIHQPWFASSDADIQKAWADMEAVQQSGLAKSIGVSNYLPSHLTALLQTAKVVPAVNQIEFHPYLQRVELLDFHKKHGIATTAFGPLTAGTKAKPGPLDDYVGRLTKKYAVGENEIYLRWCIDQDIVPITTSSKEQRLSDYLRAATFKLTPAEIKEINELGQQKHFRGFWQKKFDDKDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7Z4</accession>
    <name>A0A9W8Y7Z4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7Z4"/>
    <dbReference type="OrthoDB" id="2624308at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="563"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="587"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="788" mass="89184" checksum="27C1FFB6CC094674" modified="2023-11-08" version="1">MSSKEAQSSAYEVGPYGSGYHGDAPVSTIQHGQSYIQRAGPLQLKGYRHIFDPWTTAKWAFPNFYRKPSLGYQKLGQHRHCDVFDREWAGDYDDRYISSWDGKVSKLVVQAVDDIYDNNTGSTHRRTFIRNVAPLYLRLANWPFNHVDYSKNGGKPTSDDYPLLCLKWFVTSCIISLVISSPSPAEIAPRNGGNYDPVPYQYIGYPKVARNVLEMDGSRGRAPHQPNPVAERILRPRYLCFLREQGEPAIIMDVEEWITQYKSERNLTYVFIAYTAEQFQSSEDLRVLHQIADAAARNAGVMAYWVGCSCMPDPDQLQEDVYRICDVIRGAQSLAIAVGRPPNDVRGISTADLMLQQWGRRIWTFPEVLLAPAGKEIKVYMRGSDLLNPILVAKNQFAAKVWKDDAHVARQLIDHYEGNLILSQLELVTLALECLHKRDTTQYLRGDHSYALMGLVRIRPKIDPTDTAFQAFARLSLANGSDQLLERLICVLPKDPHQPWHSMNDAYEAKLWDILPQDVGIAGVGEDDSIILDGCRAANVRWKSFTPVAYTRRASWKRIIAQYMLRMGGVVFLLAIILLLFPFTLVIGVILLIYSLILMGLSPWLLRMLYLGKFWEQQCWFFGFEGYMDVDTIERQIFGGRLGRMKWTTNASPLSRHHRNEHGECLGDDPTSDPAVAALVEKAKTAGPGEQRIFTLVDTGNMTATLFQAERPPVCFLMAGSEGGMKRIIGCSYDWTNATMYRETVLRLGTHFEDKMSRIGRVKIGFKRSQHPFGPLAQVGEATGQEMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y879</accession>
    <name>A0A9W8Y879_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_005621</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y879"/>
    <dbReference type="OrthoDB" id="2262349at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009074">
        <property type="term" value="P:aromatic amino acid family catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052780">
        <property type="entry name" value="AAA_Catabolism_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31644">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR ARO80-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31644:SF3">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="36"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="83"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="183"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4369857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="678" mass="75586" checksum="B946D0FED4C80553" modified="2023-11-08" version="1">MSTAEETIAGLGLQDATSATSKKRTHPDDFKRAYKACINCRQRKAKCILGTGPDGGELKPPCQRCKREMRECIFRSERSWVKRRKPGETRDDEDEEAGQVQSNPAPVVQPSPGHRSSISVHGATPTFSPRFNGSHRSGILSPGHLRRISTSQPDLAHSVMRTVVSSGSDALNLLFEAAHHRDAIDSQEQSGSQAYETPRSLGQVKPSKAQSRTIGSIEALLLLSEWHPRALHFPTAADGWDCELMIGANNTTEEGSKLLEGDATSSRWLEDVIEPAKRSDRMSWMLMGCALSLAQELGLFEDNASIDKDQVSYPKFTTEFLILRRIRARKLLYVLLEQLSWRLGCTSMIPQSLNHALMEKIPVDSATGAVEQWQSFMSAWVELTKLARSVSDTIYPSTATTRSLLRTGRYIGLLEHFQPLLTSWRKKYLDPCKLKVGLHDMLHIEYQYVRIYTNSLGMQAVVERTLAETDPDVPQEDNLPFNLEPRDYDFIQEVVDGSCQILQKVCQLSETGALRYSPVRIFLRITTSSIYLIKGLSLGSRNQKLQSSLDILDAAIRALRASTLDDMHLASRYATLLDLHVARLRESFVVSSRPPRLPSRATSADVKSATTGMDFLNNHNAGQSSLSSLAENVTGLMPDDDWLALPFDPAMAPFGLDNQQSFQGFDDGTLDFIWNLPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y888</accession>
    <name>A0A9W8Y888_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA-splicing endonuclease subunit Sen15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_005573</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEN15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y888"/>
    <dbReference type="OrthoDB" id="10002170at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000214">
        <property type="term" value="C:tRNA-intron endonuclease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000379">
        <property type="term" value="P:tRNA-type intron splice site recognition and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1350.10:FF:000012">
        <property type="entry name" value="Probable tRNA-splicing endonuclease subunit sen-15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042777">
        <property type="entry name" value="Sen15_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018593">
        <property type="entry name" value="tRNA-endonuc_su_Sen15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28518">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28518:SF1">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09631">
        <property type="entry name" value="Sen15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA-splicing endonuclease subunit Sen15" evidence="3">
        <location>
            <begin position="41"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4369809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="192" mass="21619" checksum="106FDC5177217B1A" modified="2023-11-08" version="1">MTSLANAKPAIVPAPLQSFVSNPATLRSTTHLPDEQSLARQILHNLQYQHYWTELQVHTQSPVTKEPLPRPLVSGLPPQRLYVHPDEQVELLKRADSRRKAGESSTLDVKAQPEREWILPTRLNEKWTLHRLADVFDAITMVPPASAAPATEQDSKANPWRTTKRVLLGTVDSDSTVVYYIVHDGVVKPRQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8D4</accession>
    <name>A0A9W8Y8D4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_006617</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Secreted aspartic endopeptidase that allows assimilation of proteinaceous substrates. The scissile peptide bond is attacked by a nucleophilic water molecule activated by two aspartic residues in the active site. Shows a broad primary substrate specificity. Favors hydrophobic residues at the P1 and P1' positions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8D4"/>
    <dbReference type="OrthoDB" id="2747330at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06097">
        <property type="entry name" value="Aspergillopepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000024">
        <property type="entry name" value="Endothiapepsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000026">
        <property type="entry name" value="Endothiapepsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034163">
        <property type="entry name" value="Aspergillopepsin-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF23">
        <property type="entry name" value="ASPARTIC ENDOPEPTIDASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G15950)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040959692" description="Peptidase A1 domain-containing protein" evidence="12">
        <location>
            <begin position="20"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="13">
        <location>
            <begin position="112"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4368261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="431" mass="45555" checksum="EC1D219C9AE6DBD3" modified="2023-11-08" version="1">MPSLRIFFVVVLLAAIVLAVPTPAKKRGVQKRSFKVPRQINTAHPTGPNGPAAMRKVFRKYNFEVKADFTVKEGFIGLSKQNQNASSSAATDGNQTQSGSVAANPEQNAALFLSPVDVGGQKVNLDFDSGSSDLWMFSTELSTKTIGQHTAFDASKSATFKKVNGAQWKISYGDGSGAAGIVGTDVVTIGGVKVQNQTVQLANQVSQSFVEDTNTDGLVGLAFSTLNTVNNGVQKTPAKTFFDNVMGDLDLPVFTADLDPDGTGVYEFGKIDTTKFEGDLAWVPVKAETGFWQFSSTKFAIGNQTFDNPQGSDAIADTGTSLLLVDQQVAEAYYSQVQGAQLNAQVGGFVYPCDATLPDMSVAIGDYMAKIPGSVITFATVDDANTTCFGGVQGNQGAGMQIFGDTMFRAQFVAFNGGNQSLGLGQKPLAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8L1</accession>
    <name>A0A9W8Y8L1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Beta-lactamase/transpeptidase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_005753</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8L1"/>
    <dbReference type="OrthoDB" id="5946976at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050491">
        <property type="entry name" value="AmpC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021860">
        <property type="entry name" value="Peptidase_S12_Pab87-rel_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46825:SF14">
        <property type="entry name" value="BETA-LACTAMASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46825">
        <property type="entry name" value="D-ALANYL-D-ALANINE-CARBOXYPEPTIDASE/ENDOPEPTIDASE AMPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11954">
        <property type="entry name" value="DUF3471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase-related" evidence="2">
        <location>
            <begin position="24"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S12 Pab87-related C-terminal" evidence="3">
        <location>
            <begin position="432"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4369989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="542" mass="60472" checksum="DE612734BAFEC069" modified="2023-11-08" version="1">MDQHRENIKPTESIRCRFENAATEIAELMTTSGAPSLSYAVIHNGRLIHTHHLGYRNIETKLCPDNDTRHNINSMTKAVTAALVVMEVAKGTLAWSSKVKELLPDFASRSPVVEQECTILDLLTHNTGLSECDALWLGSQNNILLDRDELIKTVATLHPQSAFRSKTFVYNNWGYEIVGLVLERVTGKGISQLLQERIFQPLNMSRTSTSWYDEDDNEATAHVVLNDLTAVEIDRPKVGKGTLMAAAGGVKSTLNDLIILYTEMLSAISSALDPNISSSKTSNIFRDCIPTFIAHRVIPGESLREQTYAMGWCRAQLPGQIGRIANNAALNTPPIIGTGASPCLVIYHHGNIPGGATVVNLLPETQSAVIVLSNASAPVDIADYAAQILIERLLDVPKPVDFVALAREHVRKTFKQASDVKVELDRNRQLGTSPKALSSYVGRYWNELRNWCIDISVRNGELNMAFQGLDTETFELRHYHHDTFEWWMSWDDSCRRAREAIGVASFWLIEFEFEDGRASRLLWAMDPAVSESKEPFYFDGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8U6</accession>
    <name>A0A9W8Y8U6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8U6"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004767">
        <property type="term" value="F:sphingomyelin phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038772">
        <property type="entry name" value="Sph/SMPD2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320:SF1">
        <property type="entry name" value="SPHINGOMYELINASE DDB_G0288017"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320">
        <property type="entry name" value="SPHINGOMYELINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040980988" description="Inositol polyphosphate-related phosphatase domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="2">
        <location>
            <begin position="60"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="296" mass="32174" checksum="C8B6D6EF3B79898D" modified="2023-11-08" version="1">MHSFSIILLPLLSLLPSTISQTSGTFNAMTFNVAGLPAILNDNGVPGDKTANTARIGQLFTQYNISLIQVQEDFNYHATLYANDKHPYRTATSGGVPFGSGLNTLSNYEFTDFARVKWNTCSNIDSADCLTPKGFTFMRVKFAEGVSIDAYNLHADAGTTAADTTARASNLRQVSDYITAHSINNSVLVFGDSNSRYTRTGDIPSIFSTANGMKDAWLELVRGGVAPQAGTDALLCDNPSPNTTCETVDKMWYRGSSAVELQAQKFQYAGNMFLQENGDVLSDHDPVLVDFAWTKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8Y1</accession>
    <name>A0A9W8Y8Y1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_005060</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8Y1"/>
    <dbReference type="OrthoDB" id="443402at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056693">
        <property type="entry name" value="DUF7791"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF5">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25053">
        <property type="entry name" value="DUF7791"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="2">
        <location>
            <begin position="273"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7791" evidence="3">
        <location>
            <begin position="575"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4370539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="869" mass="98047" checksum="21CA844827CCFA2C" modified="2023-11-08" version="1">MGRPIPAVGLASALVQIIDCSINTLRKDHPIYQPSDPSTTPVENAAFLQDLIHNLYRLTDLIDQSELKKLHEEKSANKAAKKLSEAAQQLVKLSDLTKELTYPLIDALITAQAKGSFGDPRWSTAREALENGVWKKGDVTGTKKKFRALRREVETALLLAMRQYLDQSAESGLPVFTAEDTGRMHHWEKWQNTALDAIHANDWKHNKKKNVEEFSKQVDRLIQAEKEAHFYETVFKLLWFEELDDRVNSITKPIPGGFEFVFGEGGVQVGGILEWFGSSRGEGLYWVTGKPGSGKSTLMKHLFRNPRIFSSLEAWSGSSPGITSGFFFWAAGTELQRSTVGLLRSILYESLQDMIFGPLDQDDSILQSLFADRWNQFISYGGGLHAFTFSGLRRAFELMISDVSKKFLFMIDGLDEMDEYPNEVVDMIISATKKDNVKFCVSSRPSHMFQGAFERRPRLVVDEWTTHDIQIHIARAFNQEPKLERLRGKMDGTEEGKIVTTLTEKATGVFLWATLATSFLLLGLEEVDDFLILKDRADALPFLLDDLLPHILDKIEITDLEQVWKIHVLLERHSYPRLLPLSFALTAETPATFAADVRPLRPEEVARRIEDIRFQLTQRCKNLFAVFDTTPPRQPGQPLTLPDVEDLRVTYTHRTIRDYLLSQPELFSKIPPHASTLFASQQWANAHLWSLKTLSPHPAAPLRIWSPLSNALETSLALFTETKKFPLTYMHAALTTAIFLHLKAEAAGSDLPYFPITSNANSTPIQQALNMTSSLDLATLLNITSYVAVKAKGAERKDVRHAIEFNREMRKRWGKGGEEAWLGAGQGGVERLRVEYNRVRTDTDALLEYYGKAVKFGASKPAVEVPECV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9X5</accession>
    <name>A0A9W8Y9X5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_004842</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10 11">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9X5"/>
    <dbReference type="OrthoDB" id="3182339at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051346">
        <property type="entry name" value="OTU_Deubiquitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367:SF34">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367">
        <property type="entry name" value="UBIQUITIN THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="407"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="407"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="420"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4371622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="689" mass="77665" checksum="C8633A42ACF714DE" modified="2023-11-08" version="1">MSSYDIHYEEFVRTASPSLPAAFRQLSGISIRDRHQCVTQVFPALRYSKNAIDYYLSHLVFPKQCKQFPKKLSASGWDLGASKTHSTTGFSGTIDTLHLLPLDVKFLDLPSQSYTNAQVLVYLLKDETTVKLLPPCTSDMISDGEHLLTIIQGLESDVRVLLDCGASILESNNREVAEAWLLKRDNDVEAVVYFEDERLSVLDRTGHIESFQTSPYAKQLDSCIVYLDESHTRGTDLKLPRDYRAVVTLGAHLTKDRLTQACMRLRKLGHGQSVTFIVPEEIATKIRELTDKSVGDLIGVNDVICWSISETWQELKRSMPLWAVQGRRFEKSRKYLLDGANTTKSQAKGFLEDEAQGLQSCYGPRTKDDNGSSQLVDWDMSNGNIAQIVSRCRDFKASNFGSAALSEEQERELAPEIEEERQVERPPKMKAETHRIHRDLRSLVHDGIIKPGFTAFKPAFKALRSTSAAKLFRLDELSSFPTDLLVTDDFMRTVKIPYNTSRASFISDPYQRPVQFVITTPSLEYEELTQHAIVISPHEANTLQPIIQRRKKVTLHLFAARSNGAFASLDDLLLYNVGREFTPYSGSRSLTMQLNLFAGSLYLRSLTEYNELCDFLGLLRTSKAQPGQLVHADGFIEPPAGRWGLKQSPVPFLRALLMKIRREGEGIEKTHMGKLLSGVILEESDFRTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA49</accession>
    <name>A0A9W8YA49_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005458</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA49"/>
    <dbReference type="OrthoDB" id="3540210at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="502" mass="56684" checksum="E9CE25661B7DD764" modified="2023-11-08" version="1">MATPPEAEHFQSGPWTEYATGKAYILVSERTASIVTIILAFTLWLSLLQSYKLIRKLSLFLLGRIHGVNQTQGQGIVMQNLSTGATVAAITHQAVLEEPSPEQVLFRLAEDYIFVNLVQLPNPASTPRNAFTEDGSNSYELDTGWVDANELGVNESRSRVVDDVGSRYVEYLYGDGWGTAKQGNKTFGEVVRDPQRVQHEQGDYLIRGINTNAVENLQSPRKWIPELRAPGSRVSLFFVRPVNIAYTRPSTDPIFPATKSSKFTIGDGQPLYINPLQRSSVFACADTFTIRHPMHRIEWQPRYPNKALLGLPEWKDPDVLTSLHMVNVSFLARTQIASRYSDMSMHFNAPRRIRSFYSSPLESEQWKVEVKKMFNTELALLQFNVRGIAQGLGHDLPRAKNLLQELGVDARGKVLFPSQGAKNIRVAELMGFVVGCMACWILTIECKDGSGQKVLWVTTLLRSITKFWRGTVKGKFDDFWVQIKDMNFDQICEWFDEKLGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAL9</accession>
    <name>A0A9W8YAL9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Rho-type GTPase activating protein Rga1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rga1</name>
        <name evidence="10" type="ORF">N0V83_003740</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4371967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAL9"/>
    <dbReference type="OrthoDB" id="20689at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030695">
        <property type="term" value="F:GTPase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09391">
        <property type="entry name" value="LIM1_Lrg1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09392">
        <property type="entry name" value="LIM2_Lrg1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000058">
        <property type="entry name" value="Rho GTPase activator Lrg11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000146">
        <property type="entry name" value="Similar to rho-type GTPase-activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN LRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215:SF10">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN LRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="77"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="140"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="9">
        <location>
            <begin position="815"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="545"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="704"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1047"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="588"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1077"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="1099"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1155"/>
            <end position="1167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4371967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1167" mass="130073" checksum="E462FB6C149255D4" modified="2023-11-08" version="1">MGTPAEGPPPMDYQGNQQQYEQYGYDPRSNGLSRRLTDDAQSSSNSLAQQNRDYPPRSLTDPDQQQNPQPKPKRNGKICGKCGEGLTGQFVRALGDTYHLECFTCHDCGKIVASKFFPVPDKPPGQYPLCETDYFKRLDLLCFECGQALRGSYITALDRKYHIEHFTCSVCPTVFGASDSYYEHEGSVYCHYHYSTKFAQRCNGCQTSILKQFVEIFRNGQNQHWHPECYMIHKYWNVRLHSTGQPIIERLQDADGDATDEVRETVRQQEEEIEAKVNWIWKTLSAFEEKSATCISDMLLHVSNGAYVDGVMAAKKFIVHVELLFAAADDLEAQLITRTPKGLTYSRESKLLCKKVVAFFALLTQSQGTGVRRLGVTQELLSLVTGLAHYLKLLIRICLQGALKLERETRSSIGLTNFLTQVNSLDARLEEEAQRDQAAESALLVPRWADACPICDAQVEEKCLHLNNMSFNYGCMICRGCNTDLRGDVRNAYWSKSKQRVFCPACAAAQPDVEGGFEQVTKLRQYVHLLNVAHARLMATLRSSGALPHTSDDPNLTGYDSSQGHRLGDRGSELQPPHLRQDARSKSYGGTSTTESQHSTNAAYEQSMTDIKRLRSTRLDKHLSNTMKRARASRIIDGPEGIEAGGDPQVRNGMQIVQERDGQADQDNVTLAVNSLALDDIARMAALEQQREQRPNAFRAGGSALLGQDSPRLHNGHRRDFSGGQELQNQTEGRSRTFFSELSPLEYFKLKGLAVLQLGLLLDDNQYNQGELLDLIESKKNNFWGKIGFGKAKNKPKKNTTGVEKPVSDKATFRQSLEYLVEKYGDECTEGVGPGALKVPALLQDCITAMRSMDMSIEGVFRKNGNLKALRELEEEVDAKGVQQVDLNTKNPVILANLLKRFLRLMPEPVLTLKLYRLFMTANDIQDEEQRKKVLHLVLCLLPKAHRDTMEVLFCFLNWVSSFHTVDEESGNKMDTWNIATVMAPNILRESNEKEVKNVDQGAVRVVFDLIENNDEFSEVPPEIMELLSDDHASDMSAKEIMRQWEQRGKNPVAAAPTPSPRNQGGTAKRKDERNAPQITQADNNPQALAGEASVRQIPGGGGGGNPALGGPPSSQQQSPVLGSPNPPYGQNPKASAESHRTASPHRHSYRSPAFQKQTQIGTAGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAN4</accession>
    <name>A0A9W8YAN4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_005744</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">RNA-binding nucleolar protein required for pre-rRNA processing. Involved in production of 18S rRNA and assembly of small ribosomal subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAN4"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07920">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033712">
        <property type="entry name" value="Pumilio_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF48">
        <property type="entry name" value="MEIOTIC COILED-COIL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="6">
        <location>
            <begin position="373"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="435"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="471"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="619"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="663"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="113"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="157"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="113"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4369980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="752" mass="83492" checksum="B3C5D26AC647868D" modified="2023-11-08" version="1">MSFNITSEGSPTPRKGPTRLSMAGDLRIDTSPEVLGQAVGNISQNHYPYNLHRAFGSSLTSNSQGMKRPAGSSTLGGQPMLLQQTTLDGYKKSKMVSHEEAAVMDSLLQRLNGRSSPQDQLTSSNGSVPITPATDEFASTPPSELTDSVMLVDASALQKLKLELQEARNEVNRMNQEMHSQHVARSTMEHLSQSSEADYGYTGDVTEQTLTQLQNKFNASTRSNYGWGNEPVRPTYNSNNSFGPPYQAQSRLPPAAQSGYRRSGFLNEPTHFPLDQTFRGSGMSSGLTNGMSNSFTSGMTNGFGNGMNNPPSRPGSAFDPLYTQYAVPPMYSAGQMAPIGTMGSRLSPDASEFNLANGMNPSPWNSQTPSDTGVAQYVSPAEPMNYRRLLDRNMSCNWKYIVDKIICNNDQQASIFLQQKLKVGTLEQKYEIVEAIISQAYALMVNRFGNFLVQRCFEHGTNEQIIAIAQAIRGNTLSLSMDAFGCHVIQKAFDCVPEEYKATMVHELLRRIPETVIHRYACHVWQKLFELRWSDSPPQIMRYVNEALRGMWHEVALGETGSLVVQNIFENCLEEDKRPCINEVLASIDVIAHGQFGNWCIQHICEHGAPADRSRAVDHILRFATEYSMDQYASKVIEKCLKIGSGEFLDRYLERVCEGRPDRPRMPLIDIAGDQFGNYLIQHILTNSSAHHRELVGSHIRKHMVSLRGSKYGSRVAMLCCNPALATRPGPPAGMVPRYNNPVPRTSFGAFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB30</accession>
    <name>A0A9W8YB30_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB30"/>
    <dbReference type="OrthoDB" id="4226666at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052973">
        <property type="entry name" value="Fungal_sec-metab_reg_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35392:SF1">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35392">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="321"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="877" mass="99173" checksum="0ACB46A7C6E4F768" modified="2023-11-08" version="1">MSPYSQHYQHFQGQPLFIDPHTFVYGHHDTRGSKTPTDSLINHTGLSPGPLTTTPPLSRNPSQPPEPSRDQLPDQFCDNGSLSNSPTSVRTPDGESFEVEMLDSDSLRNFYYQNGNTMSTQSSHNAIPAVDSSMFFTAQGTISDQAIQAAFHATIASEPQQYQQQYNAQTQQPYHISTIHQQQSNFYNQSYTTQPPRHDPWNGQGPSRSSMVPRPGHVVFDPATDPVNYSEYVNEVNDVDLWTMNYTDPSYLVSPSEPTIPPQDNSFPITAQNLTHRPHNHVHHTHAQNQSPVEMHLTRASPPPDQQNFVNYNASSTLFTENYTTGNQYPPTSPASSSIGQHPRSPYQPAISPTVASPGSSDGMVSSYQHSDSGMMLDPYHPAQQYHNILVPQVPSMKVDYSPTRGVPETSFDASPEPESSRAGEAARALNKSGGRALGTHLEPTVAKAAHDMRKIVACWHCVLQRDKCGPGDICERCLKRSQRPNADCGLGCTRIKLVDLSAYFLPSLVTQMHEDSNLTHFVSQYIHQWGNVELTVWMTCGQTHMPRIPVKVYEFVPRGDALLKQIQYKSDPVTHERIAVEKKSPALGMVHINHNEEKKYDKYIADIVDNHLDAFGELCWMEDDNDFQQKLFRLMTRVKPKSDDEAKLLREVFRLIVVTFIMSHTLTIAEETKISTLQRMHSYGGHNSYVDNFTSPRMTNRQLKYFFSRLQRSIQATVLNKLQQMFKSSKGCDKWLAAFVAVLGMCMALEDQQKTIHLVMSTKATTEKLDQRDAQGQADIACREIDARMHFIQQIFRWKYNRKCNPMRNAEHDWDKEVGFGDASSVNFVRQVAQLVKDNTDFLQQRQQVSISPANQTKYTSRLVGQFLLSFWLPNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB92</accession>
    <name>A0A9W8YB92_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PSP1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004760</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB92"/>
    <dbReference type="OrthoDB" id="243127at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047767">
        <property type="entry name" value="PSP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007557">
        <property type="entry name" value="PSP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43830">
        <property type="entry name" value="PROTEIN PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43830:SF3">
        <property type="entry name" value="PROTEIN PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04468">
        <property type="entry name" value="PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51411">
        <property type="entry name" value="PSP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSP1 C-terminal" evidence="2">
        <location>
            <begin position="570"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="365"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="902" mass="97499" checksum="2FD6A40B1954C1BE" modified="2023-11-08" version="1">MSSNTFKGGLTVGGSALDKSKLQMRRPTPDSDVLASSDDDREHVPPIVRPTAGGYPGGRRPSSGWLQDIQPNRKFSLPSVSFAGSQPTTPSLELPQQARPSTSAFPWNTTSFTSSQSNSRLKEVLPSPTSAHAPHDKPLPSPTTIEGDDGIGFLLNQQNPTPLRKSVRSQSYSIGQGDIENSPVGQFSAARLRSGVRHRPSKPSLLGESAIGLSQLREDDADEVESSNGSEHGVRLPTGYWEREQKQALLKQAAVENARARNRATSTGSPVSQQRRKTTAGLRNVSYGNTTEYAIEEHDDPETTMNTPTLTALTRRFSEHVSVLGREQDADVVENSQKWATGNIPPITVDALGRRHSFAHYGGYNPSMPMPTLGHTQEEDEDLLSPRQSSQSPDENEPFDAAAYFTGYGPASRAINSSAISAAHPDPIIPNTAMSAGNPYAVPHVLGRPGRRLFVVTFKCSRADIYYLYDNTGLEIRRGDLVIVEGDRGCDLGQVTHADVSLEDAKKHKAEANEEHFRWLVMFSQYSLAGNANDSGMLGALYRANGFPNPMSRSQLTGMGAQQEQDAKPKMIKRLAQQHEIGALRDKEGHEAKSKRLGAQKAADHKLPMEILDAEYQADYHKLTYFYYAESYVNFNDLVTDLFKQYKVRIWMSAVNPASVVNPAGLTQIPPPSAIGPGAILNSNTTNAPLSVGPGFGSNNYRSNEQYGRGRNNVSYTNYDDGYGAFSHQLPSYPPQPAYNMPPWAHGQQLAPQMYDRYGAYAMQTGNYPNVTASGSPMNYGAYYPPTTYAPSPAYNIASSGPAYRGGYPATSAYNASQSYTSTAGPSYGQYTTSGAAYTSSGAGHPNVYGSTAYTSGPTASTSYSTSGGYGNGYTAASGPSYNTGYDPAFLTAMHNLSFGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBS5</accession>
    <name>A0A9W8YBS5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SNF1</name>
        <name evidence="16" type="ORF">N0V83_003393</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBS5"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14079">
        <property type="entry name" value="STKc_AMPK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14334">
        <property type="entry name" value="UBA_SNF1_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000544">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000069">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000236">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032270">
        <property type="entry name" value="AMPK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013896">
        <property type="entry name" value="SNF1_UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16579">
        <property type="entry name" value="AdenylateSensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08587">
        <property type="entry name" value="UBA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="62"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="414"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="491"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="588"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="690"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="427"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="458"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="601"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="627"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="719"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4373102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="893" mass="99683" checksum="38FBC93856443F60" modified="2023-11-08" version="1">MSSAIDNEDLEELSMSMPPPRRGGASTTKTVDFSPAPSTALATTANESRSRDSKANQRLGQYTIVKTLGEGSFGKVKLATHQVSGQKVALKIINRKRLVTRDMAGRIEREIQYLQLLRHPHIIKLYTVITTPAEIIMVLEYAGGELFDYIVNNGRLQEDKARKFFQQIVCAVEYCHRHKIVHRDLKPENLLLDDHYNVKIADFGLSNIMTDGNFLKTSCGSPNYAAPEVISGKLYAGPEVDVWSCGVILYVLLVGRLPFDDEYIPTLFKKIAAGNYSIPSYLSPGAVSLIKKMLMVNPVHRITIPELRQDPWFTKDLPTYLEPPVQEFYDSGADPNKAIDPKVLAPSAPAPVVQKLHETVVTKLGKTMGYAKHDVQEALARDEPSAIKDAYLIVRENQIMKTNPLLTNEKNLQPFFAQSPPSHHESYLSTSIPNTLSSISRPQVIPPASSSEHERARQGSNASSQLANVRSPVSTVAILPSSLTEYHKAYMKGHPRPLNNPQDDALPNSEQTEEQRQISARRLKPNFRATPESHRQRPEPMTSLPAKKARPTKWQFGIRSRNQPAEAMLAIFKALKAMGADWEIPKIRKAGGRSGSRSRSASRESQERSRSRSRSRSRGSSISSHSSQEDQGDRFNSPHREPLTVRNGSADEQETRGRQKRHYNHTNDWGYHIPEDPWVINARFRKEGMFPPGVAHPSSTHSSRVDLASDPSGLRRRSSTNTSTSSLNQNTENMTLAERTAAASAGEDYPQPDEAVWIYMSIQLYSIDRDFFVVDFRCAGYERLVSNLVREIKASSTSNTSISNLALSSSQPAHHHHEDGWDDEQGVWRRLGEGEPLPEDLARELRDGTGKGVLRERTEEVGAGRRKDGEKIVTSPFPFLDVASTLILQLSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC19</accession>
    <name>A0A9W8YC19_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">methylmalonate-semialdehyde dehydrogenase (CoA acylating)</fullName>
            <ecNumber evidence="2">1.2.1.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ALD6</name>
        <name evidence="7" type="ORF">N0V83_004028</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.27" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC19"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004491">
        <property type="term" value="F:methylmalonate-semialdehyde dehydrogenase (acylating, NAD) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006210">
        <property type="term" value="P:thymine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07085">
        <property type="entry name" value="ALDH_F6_MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000002">
        <property type="entry name" value="Methylmalonate-semialdehyde dehydrogenase (Acylating)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000003">
        <property type="entry name" value="Methylmalonate-semialdehyde dehydrogenase [acylating]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010061">
        <property type="entry name" value="MeMal-semiAld_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01722">
        <property type="entry name" value="MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866">
        <property type="entry name" value="MALONATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866:SF3">
        <property type="entry name" value="METHYLMALONATE-SEMIALDEHYDE DEHYDROGENASE [ACYLATING], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="6">
        <location>
            <begin position="54"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4372254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="543" mass="58350" checksum="4F496FB18B224BFD" modified="2023-11-08" version="1">MSSPASSNKVAARRLHATARHLKPATSAVAESYPMNHEPIQSPADTPNFVDNQFVASKATQWIDLHDPATNNLVTRVPQSTDEELQAAVDSAEKAFKPWKETSLLHRQQIMFKYTHLIRENWDRLAASITLEQGKTFGDARGDVLRGLQVAETACGITTQMTGEVLQVAKDMETRSYREPLGVVAAICPFNFPAMIPLWTIPIATVTGNTCVIKPSERDPGACMILAELAEKAGFPPGVVNIVHGAAKTVDFILDEPRIKAISFVGSNKAGEYIYSRASANGKRVQANLGAKNHAAVLPDCNKNHALNAIAGAAFGAAGQRCMALSTLVMVGETKDWVPEIAERAKGLQMNGGFEDGADLGPVITPQSKQRIEELIASAEAEGATILLDGRGQKPPSEKYANGNWIGPTIIANVKPHMKCYTEEIFGPVLVCLNVETIDEAIDMINANEYGNGTAIFTRSGATAGRFQHEIEAGQVGINVPIPVPLPMFSFTGNKKSVAGGGANTFYGKPGLQFYTQQKTVTSLWRSEDAVATKASVNMPTHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC46</accession>
    <name>A0A9W8YC46_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_002754</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC46"/>
    <dbReference type="OrthoDB" id="4150765at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046707">
        <property type="entry name" value="DUF6780"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20571">
        <property type="entry name" value="DUF6780"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="3">
        <location>
            <begin position="186"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="43"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4374015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="762" mass="82494" checksum="8565D9D9ABD6FF0E" modified="2023-11-08" version="1">MKREEDLDQSLPGSQSTETIGRSNDSTFGVQSLADTLEAAFGAESTAAGKRTEACNRTKGHGKSTSRAGSHSSSAGSVKPLESSSSSPVRKLKRKLSHHGSPVPFTLPTADTPSPVLTSAIPSPPKSASVHSLKLSDEESALDDVASQAITSGGEEEDADVQEGPLLQLPTASDAQYTDARKEAIYKQRQGYGQTEDNGVGKTSLIRSIVQLCEDIVHVDPLSPSQSFSQPPPPKSKSRKRKAEQVNGTTRITEIHASTKPYPHWWTDVEESRVLRRRKSSIDTVLERNVCFVDTPGCGPGASEKDDMNLVVDYVESLLYQTSSIPSMEDGDVLGVVSGNGGVSIDVVLYLLPPNHDISKDVEFMHRLSFLTNVIPVIAKADTLSAQEAIALKTSILARLQTTSVKPFFFGKALDDALLAVQGLPLIHSQPTSQPVVQLEKWAEASQFPFTTPTYPYAISSTPGPDNENMDASLLMSPDYVQPLLPSELAALVNQVFDPESIAWLRHSAAKKFLAWRRRTQLSGDSFMMQGLQQPRSPTTASLGLSAAAMNTSSIFSAASPSGVLVPRSSSPFYSSNLQSPLLAASASLANSDTSDYPTEFPLTRYTKYTQGEQRLSEVSIAKWATDLQRSLRNERERFEDLQRNDRAKWLLERVGEEVSRGTIVTSPGGSPRAEWAVVRHGDEKASKASQRYNRATGLDSRDPLGLCNFSDDLRKRGFVLVKVLGGMSVLGAIVVGIVRAFGMETGLPQGGWWSWLTGGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC71</accession>
    <name>A0A9W8YC71_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SRR1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC71"/>
    <dbReference type="OrthoDB" id="5318346at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012942">
        <property type="entry name" value="SRR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42080">
        <property type="entry name" value="SRR1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42080:SF1">
        <property type="entry name" value="SRR1-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07985">
        <property type="entry name" value="SRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRR1-like" evidence="1">
        <location>
            <begin position="57"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="252" mass="28327" checksum="602686104FBBC773" modified="2023-11-08" version="1">MSSAPVSEAVQAFAQLTRNRKSKVKSSSREWQYLSAMLQDHQALFEQSQLSQNISRQLKVTLKNSSTSVTTILSLGLGSLQVTREQTRRLKQLTILLSLRDTLRQVSGKQIGIYAQDPTFTRTDETFLNSLGISILRTPSGSDLGEAAAIICPSTLIYSPFLTLEAYEQLLFNAATPVQFFVGDDFNALLKKWPKHSAERAQVERVMKSGLSRYRRRAVDGEGFWKEQDGTFPMAVYGWVDGGERRREKAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC74</accession>
    <name>A0A9W8YC74_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWR1-complex protein 3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002799</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC74"/>
    <dbReference type="OrthoDB" id="5338195at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140849">
        <property type="term" value="F:ATP-dependent H2AZ histone chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037651">
        <property type="entry name" value="Swc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057558">
        <property type="entry name" value="Swc3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28108">
        <property type="entry name" value="SWR1-COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28108:SF1">
        <property type="entry name" value="SWR1-COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24707">
        <property type="entry name" value="Swc3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SWR1-complex protein 3" evidence="2">
        <location>
            <begin position="64"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="590"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="470"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="590"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="636"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="645" mass="71131" checksum="1C2DB0550D8EFEC9" modified="2023-11-08" version="1">MSEPRRSTRARAREEAAPETPKETPVKAAKPSLKRKRASIVVKESTPLTTVTEGEPVPPKQTLPLKLVEGQPLPTLPEPQPLDLPSSEWQNIQQSGVLEASLERSRAVWTSGVNFRTFHKHFVQPKKVADRTDEDKTKNQRQKELLRNFPQIGVSSEKEKEIAVAQLVIEPHTFNIRLFGPREMAKSVPKKAPISNYGPWPNHSQHGQHSQPNQYTQYNNSQPVYKPPPQPRPPPPPPKPVQQAPAPATSTPAPDPVIHMLAARAGTDPELKAVMKIVANGSASKEQLEFFQTHINELTAILARQKEQAARTQKPAAPPPPQPKPLPPPPLPKPAAAPPTPRPVQPIYQPLQHTPKPYSPTPHQAQVQQAPHGHQSHSPYNYNPPQQYRQPQQAPSYSAPPPRTSYRPLVFEFTEGNNDKLYFPSYSFMEWLPNGSGVKISFLITKMKPKPEPAVKPPSTPAPKAVQTPLPTPGVPPSATPDGPQPNDATPTPNPNPATPAALPPAVPPTPNPTPYVPPPRIEDFDEKNDIADIEFYQPVTVLLLTDDPEIIRSLPRAVRPPDVVEKYMEEVFDKCKRAEETYLAFRLPKDGEEQSEKRVRSGDVTPAVATPTHDVHMGGLGANLGMSSSALEKKKAGRPRKSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCA3</accession>
    <name>A0A9W8YCA3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6606 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004838</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCA3"/>
    <dbReference type="OrthoDB" id="3182339at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046541">
        <property type="entry name" value="DUF6606"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20255">
        <property type="entry name" value="DUF6606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6606" evidence="1">
        <location>
            <begin position="8"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="269" mass="30078" checksum="9D98E781F3973EAA" modified="2023-11-08" version="1">MAPVAAPERCLLKMVIQALQDLTNLVNKSYLDTVTSAITMIKNLRDNRDGHGDVSEVQLEALISDLAAGKTRSPVPLEIKAQNACILISRQNGHLNFEFFELSPTNEAAMRSTRLTRTFPGYASRVAVDQMMNPSRQKSIAGTIAKMATQSAPGLQPQVRKNGKIEDEDRDTTAPSLVTDFLMTVIAAIGETTEVKRIMKATREDVLWDDCKLPWRRSPLWLLVRVVLQLWFSRNATCLQSPDELYKAFMICMLSQLLQAVRANIFSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCE2</accession>
    <name>A0A9W8YCE2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_002756</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCE2"/>
    <dbReference type="OrthoDB" id="5667at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF224">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="59"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4374017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="452" mass="49073" checksum="CA5B3F42AAF932C5" modified="2023-11-08" version="1">MSQTPSATSFTDEEKQQRMNNDVEQPPISEKPAESAEPATAPAAVNPFHPSQFPDGGKDAYLCLLGGFCCLFCSFGWLNCLGVFQNYYQMNQLSEYSPSTIAWIASLEIFIMFLPGPIVGWVYDNHGPKYIIIFGTFMHVLGLMMTSLCTEFWQFILAQGICSPLGLNCIFQAGTATIPTWFFKKRGLAYGIMAAGSGLGGIIFPIMASHLIPEIGYGWTMRTFGFMILGLMCIAGLTVKSRLPPKPRPLQLKVFLEPFKDIKFVLLTISSFLFFMGLFIPINFIEVQAISDGMSFRLAGYLLAVLNAASIFGRIIPGALADKVGKFNMQALWCTVAGIIVLALGLPASGNAAYIAFAALYGFASGAYVSLLPAQMAAISKVEQIGVRVGVTFACVSFAGLVGNPIAGAIVDHNHGKYWGLNVFSGIMLLSGASLFLFTRMYVANWKLLARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCG1</accession>
    <name>A0A9W8YCG1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_002640</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCG1"/>
    <dbReference type="OrthoDB" id="10255013at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15849">
        <property type="entry name" value="SNARE_Sso1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF380">
        <property type="entry name" value="SYNTAXIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="5">
        <location>
            <begin position="216"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4373901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="335" mass="37295" checksum="6B5320D70989569A" modified="2023-11-08" version="1">MAATNEQYGNKGYGQIGYGNNPYDQRDDGAGGAGGDRYNNYSQGRYDDPALEMQSYNGGGGADPNAILNECREVDRALDQLDDQLNNLERVFRQVLARPDMPSGEINDFTSQVMTGYRALVSRVKNIKSKPESGSPRNAPQVGKVDRRLKATINRYQTLEADFRRDSQAAAERQYRIVRPDATDEEVREAASDPNAPIFQQALLSSDRRGQASSTLRNVKERHDAIQNIERQMVELAQLFQDLDQIVQQQEPLVMNIEQKGEEVHENVVQANTEIGGAIVKARSRNRKKWWCLLICILIIIVIVVIVVVVTQVNKAAAPKAKRALAGTSLLQILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEW6</accession>
    <name>A0A9W8YEW6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Vacuolar import and degradation protein 27</fullName>
            <ecNumber evidence="5">2.1.1.221</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">vid27</name>
        <name evidence="5" type="ORF">N0V83_002215</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.221" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEW6"/>
    <dbReference type="OrthoDB" id="10251113at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052905">
        <property type="term" value="F:tRNA (guanosine(9)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040458">
        <property type="entry name" value="Vid27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013863">
        <property type="entry name" value="VID27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040979">
        <property type="entry name" value="Vid27_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040768">
        <property type="entry name" value="Vid27_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913:SF0">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08553">
        <property type="entry name" value="VID27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17748">
        <property type="entry name" value="VID27_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17747">
        <property type="entry name" value="VID27_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Vid27 N-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Vid27 PH-like" evidence="3">
        <location>
            <begin position="246"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar import/degradation Vid27 C-terminal" evidence="2">
        <location>
            <begin position="423"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="375"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="766"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="183"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4375131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="800" mass="90133" checksum="EBBFF68018030747" modified="2023-11-08" version="1">MFMLRNLSKFVFGDGSKETIVEIAQGQLYLVRPRSVKGYSELIFKDAAATIRRTGQEFQYQLVVQRAYEEGEEDLLAEEGGEDAVLDALAGDKDEKTFLLDEDLQFRDDVRSTGEKIFAWRDISGDLGDLWEFVCDPSVKPETAALFERVALQCQYERKFRRSHEHATEEDLDALAYYEEPIPDASPVASPSSKALPAPPTAEELFPNTTKENMVKKVAGKGKALQVEEESTAAPPSAAQPDARETLAEGVAELHLFDFPTGTFIKQDAKVHATVTEIGNWNYWLHIVGEEREWLGQPIVDDINPVFNYEFKSFIFNHYLDDGSAYSWLLRFTEQEELENIQQGVMQALWEHLNQIKWQKAKDTDREYVLEAFQDLTMEDAPEEEQEEEEEEEYESANDQRSEHYDSDESEDDVDTAPKDGAENSQLAVGYKHDRSFVVRGNKIGVFKHTADNQLQFSTTISNVRTPKGKAFNPNKVMLHQQDQDMILQNLDNPNALYRMDLETGQVVDEWKVHDDIPVKVFAPENKFAQMSGEQTFLGLSGNALYRVDPRLDGNKLVDTQLKQYATKNAFSAAATTEKGHIAVASEKGDIRLFDRLGINAKTLIPALGDPIIGMDVSADGRWVLATTRTYLLLIDALQKGGKNDGKLGFEKAFAKDDKPQPRRLALTPAHVAQFQHETKAPISFTTARFNTGLDDKETTIITATGPFIVTWSLKKVLANRRDPYIIKRYAEEVKADNFKFGSDKNLIVALPNEVDMVARRALQKPTRESIATPSRVGANRRSGVRGSYLGRNDIVNSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF11</accession>
    <name>A0A9W8YF11_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Arrestin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001509</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arrestin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF11"/>
    <dbReference type="OrthoDB" id="3892815at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF17">
        <property type="entry name" value="FI21816P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="350"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="364"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4376226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="440" mass="48961" checksum="F3B0E2522C536C6D" modified="2023-11-08" version="1">MGIFSSSKLPQPPLPDLAIHLSAPAQKVFQPDDIVTGHISFTSVVPIIPRVLEVSLFGQSLVWHRTSHSNSNNTTDYHHWRDKAPLFEVATNVLSSSNLSGKKEGALSCAYEPGQTYTFPFSFRFPAGTGNTRFGQYKNEADERWTVTPHDLPPTFLHTDKNGGTDNPNYAKIEYGVRVRLACPGVGVVQGKNMVDLTATAPLLFQPQNQSRIQQIVEGHSPTGGLIRHAKDFTFQSSLLSRPTTGQAASSATTTLSFRQSLRDRFSSHTPKLTFSTALHLPSHLTCGSEFRFRATFTVLSRSPTATHMPSPIVFRVLKLELLDFTFVRAPRDWEASTWRDGHHRGNKWEFWPPPNGHPFSGEGTKDRKGVEGVDEQEKEKEKEMDMGQSGEVWFTARIPGFTPPSFKSFAITRVYKVKVKLGVELAGKQFVLEAESHVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF40</accession>
    <name>A0A9W8YF40_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_002374</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF40"/>
    <dbReference type="OrthoDB" id="444325at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061632">
        <property type="term" value="F:RNA lariat debranching enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07380">
        <property type="entry name" value="MPP_CWF19_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040194">
        <property type="entry name" value="Cwf19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006768">
        <property type="entry name" value="Cwf19-like_C_dom-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006767">
        <property type="entry name" value="Cwf19-like_C_dom-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12072">
        <property type="entry name" value="CWF19, CELL CYCLE CONTROL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12072:SF4">
        <property type="entry name" value="CWF19-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04677">
        <property type="entry name" value="CwfJ_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04676">
        <property type="entry name" value="CwfJ_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cwf19-like C-terminal" evidence="3">
        <location>
            <begin position="306"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Cwf19-like protein C-terminal" evidence="2">
        <location>
            <begin position="479"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4375288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="541" mass="60388" checksum="27A44B8E6DE8B3D3" modified="2023-11-08" version="1">MASKIVVVGHVDGQFQAVFQKLSSLHAKNNFAFAIIVGNLFAGSWEADGADQTNIQSLVDGKIEVPLPTYFALGNHPLPPPAVEKLESNSDELCHNLFFLGKRTTMKTSEGIRIVALGGQLDPNIIAGQSKDKYPPFYGESDAKVLRGATTADILITSDWPEGIQKRSKVPFNPEGQPSAQQCIADLDAVLKPRYHFSASGGAFYEREPFFHAPSEETNGLYPITRFISLAPYGNPNKEKWIYAFSLEPSASHPVSPPSGSTACPLTLSEKKRAAPEHREQTLVYDDGSRGRRSHKRRKGETRGPISASECFFCLANENIATHLVTSIGENSYLTTAKGPLSTSQTFPKLGFPCHMLIIPFVHQPTIGSMEEEERRATYSEMQRYRVAMNSMLKTVSGAEYGSVTWEVSKSSLPHTHWQFLPVLADLIQKGLVAAAFKALAENLHWPNFKKEDVGDGVEETSDFFRVLVWDPNDEPGKQTSYIMRFDEKIRFHNQFGREVLAKLLRLDQRIDWRDCGQTQAEEEQDVEKFKAAFKNHDFAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFB1</accession>
    <name>A0A9W8YFB1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_001659</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFB1"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04077">
        <property type="entry name" value="Peptidases_S8_PCSK9_ProteinaseK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000007">
        <property type="entry name" value="Subtilisin-like serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034193">
        <property type="entry name" value="PCSK9_ProteinaseK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF58">
        <property type="entry name" value="ALKALINE PROTEASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040765197" evidence="8">
        <location>
            <begin position="22"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="10">
        <location>
            <begin position="36"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="9">
        <location>
            <begin position="166"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4376376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="413" mass="43371" checksum="62D5DEE4493C4D3E" modified="2023-11-08" version="1">MQFFTRALALAATAVPFLIQAAPLPSSPTNDLIAGKYIIQLKPDTDIASIAAHHNKVHDIRARNLARRESEGEVSAGVEREYDFGNFKGYAGSFDDATIEELKALPEVLVVEQDFMMYTTQENSSTKALVTQDNAPWGLGSISSRTRGATSYIYDASAGQGTYSYVVDTGIRLTHVELEGRAVWGYNAVNDINTDNAGHGTHVSGIIGSKTYGVAKNTTLVAVKVFEGTTGTASTVIAGFQWAVKDIVAKGRTSTAVINMSLGGQGSTTWDAAITEGWNQGVLAVVAAGNENTLASEKSPARSPETLCVGNIQSNDVRYQGNTGSNYGPAVDIWAAGTGIISTYASSDTATTSLTGTSMASPHVAGLVSYLRGLEGAKDAKAIKARVLELATPGRVSDEMGAANLLAYNGNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG54</accession>
    <name>A0A9W8YG54_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG54"/>
    <dbReference type="OrthoDB" id="3682662at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="25"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="135" mass="15408" checksum="F4367264F69DC41A" modified="2023-11-08" version="1">MTTSYTAHRAALLSTTFWEVLPSHYDKIQNRRSKIARLYDEAKSELLATDREVAMNSLKAELEMLDNDVNEYRNVTKGVDITDIAGMYVTAGKSPHRALQVAKEDFENLEINLRMIEERIAELKADIVYGLGEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG73</accession>
    <name>A0A9W8YG73_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001756</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG73"/>
    <dbReference type="OrthoDB" id="3798510at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="471"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="412"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="517"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="537" mass="59238" checksum="7B52AF3D518200A2" modified="2023-11-08" version="1">MAHSEQKRPGRQKSNSMSELRNRFAALRMKDDRPALEPPLGHRDHQTNSEESSPVSLTESVRHHWAPQFRPNSATTPASPKQAGYHAGPPQPPEPCFVRSKLRIQKLDADRVERKDSHLYPLSAFLTCPDLTDRYPSTSKFLDTAHTSLGNSDSKLINAPANPTDPELSAAWAVCTALDSKKPEPTSAQSAICMQEVELLCPQQPTQSPPSQCPPASSHPTLAMERLTPNPSNVQTYRPPVPRPTGQESTPSSVLPQVSEPLVQPLLGLQQTSSLPPHYIREGPQATFAQRIKALREHHNLCNSSASRLVRAPIPYPHLFIDAPKRQPPPAIPTNLFNKSHVDLILARQKASRRTLSPKAQKELDDFLERGHANPCWCSRHMKPASASRNTATVSCTTSMEGRYDTNEDDPAAASSDDSAADGNIDMYKVDTTPPTETPRSEFDELYDMMFESTKIEDDWTVVSPSARYRKLRSQSLPTSPVSDEDNELDMASYLPSPPLTPNRPKYTASDPGTDVSTPSPWILSPILSPSDSVQSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHA6</accession>
    <name>A0A9W8YHA6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000916</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHA6"/>
    <dbReference type="OrthoDB" id="3438340at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021641">
        <property type="entry name" value="DUF3245"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11595">
        <property type="entry name" value="DUF3245"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4378085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="180" mass="20039" checksum="63A42C413816F6C3" modified="2023-11-08" version="1">MIGGILPKDIADGSFTRRIPTSNDKLLEQLIGKKRAKAHLAAKQEAARPNAKVQRHGKPATQRKDESEDEEEGRAATFKSKRRKGTEGRSVVTKKEGSDDENEDEESRTKRLEGGMSSEGRKKEVDNAVEIKDEQTEAVEEPEGASLNKKAPSRSKAKPKSFLDEILAERSKKKNRKSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHG0</accession>
    <name>A0A9W8YHG0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHG0"/>
    <dbReference type="OrthoDB" id="5125733at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112:SF16">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="207"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="803" mass="91297" checksum="AA8586283C4A5D2B" modified="2023-11-08" version="1">MFCNRCQEFWGEALSRAEIPQIINSCVDVRWNHHEIVLHQSIRDLKTASDLRCRLCRIIYYTPTEYEHDKLLKDVDEPLDVVLSLNTANGPHPVLMAEFQAKSENGTQPIIPKRWVASCSGLFSDDDLATTLKRSVETENNSTGSDAALELAAFWTKTCINGHEKCRAPTSHGTTSFVPTRLLDVSNGAIRLIESKSEIRDGTDRSFIALSHCWGLIPIIRTLTSNYDDHRKGISPERLSKTFMEAVHTTRKLGYRYIWIDSLCIKQDDGADWAAEAATMCDVYQYAALTIAAAHAPGGDVGCYAERDALLHLPFFIELPESPASSKATRIYFTSYGRVKDVGGGDPALYGRAWVLQEQILSPRMLIFDGSQIKWECLTMHGSESSPTSGTTRHSLYHKHIRSGIMDDFEFFDTPADKTSEDRGFWARLKHQYWCYIVMDYTHRGMTKSRDRLVALAGIGKALSRHTKNRYLAGLWSEHFTTGLLWSLAHNEKFTMSAADNFDVDKNEKIRHEEDLAPSWSWASVTAPVMYASNELLNYDRICNVVNVSVSGDIDKQTGRAEICGHIRRGYVNAVYPYAIREAAAEYPHMATIKPAGNLGREEMKFKGRMFHPNDWFLFSEKSPSTGKSDASDQHLTTHGNFRLVRGTFKPDQIIKPSQEITFIAIAQQHFGAQLETRLASHQDDDALKVHSLVLLPTSRVPGEYKRVGLAIWDECAWYGYLCGWKDARDRIVERPGRWTEEGKWSKEGWLDKVGRKLWWDDLEFYEETKKGDHQHEYEANCLPDMAKYHKKVVVEEKTVVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHM9</accession>
    <name>A0A9W8YHM9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_001060</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHM9"/>
    <dbReference type="OrthoDB" id="2988756at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF146">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="30"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="289"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="326"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="299"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="346"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4375783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="377" mass="42165" checksum="3CF613294533C450" modified="2023-11-08" version="1">MSSGNNWISQGELLRTDYGMIAFTSTVFVARVIVQVWRRKKVEMQDVWMYISFAAYLSFCVCYVVITPTFFQIDAISAGEIAPWAGMQRDIQLASEIMWSSGMQYWTCLWCVKFSLLSLYKKLLVGMPNAYLWTWWGTLIFAIITYITCIITGPGLACNDTKAFFDEGKMCLSPKEVRQQTANLYYAYAVDTLTNLMVMFLPIRLIWNLQMARARKIGCMLLFASGFVCILFSTIRIVQVGGDGKPRSPDAKWLTMWTIIECSTAVIIGCCPVFASLIPKTSTTNRVSYDTQGYVRQPGSGSNRSAPATASASRGVKLKSLISSKSRKAGTGNGSLFQDENGSQEELAKSRDDGRGIKVTTQLRQEHERRMASKSTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHV6</accession>
    <name>A0A9W8YHV6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_001181</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat MET30/SCONB/SCON-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHV6"/>
    <dbReference type="OrthoDB" id="5580488at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22147">
        <property type="entry name" value="F-box_SpPof1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000016">
        <property type="entry name" value="E3 ubiquitin ligase complex SCF subunit sconB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000715">
        <property type="entry name" value="F-box protein MET30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051075">
        <property type="entry name" value="SCF_subunit_WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19872">
        <property type="entry name" value="UBIQUITIN LIGASE SPECIFICITY FACTOR/HREP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19872:SF9">
        <property type="entry name" value="UBIQUITIN-BINDING SDF UBIQUITIN LIGASE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="8">
        <location>
            <begin position="208"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="391"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="431"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="510"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="626"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="651"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="691"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="317"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="580"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="580"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="592"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4375903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="735" mass="82358" checksum="7B694640F05B5CC9" modified="2023-11-08" version="1">MASNTTHTIEAVATDRHRSQPKSDTPKRRRLSWRTQVPLSDNFTTATSTDPHAALMHGHDHEGLSERTRHLHGESVEDTEEAAASKSKKIEKMITPYLAQHIPHQYNPLGGNSDGQEAPPTTANTKFCYRHRPDLLCRRQADEPSMEQLQDELGRESQADQQSIANVWSLFSAAPSRHRNLMLQGILAQCCFPQLSFISSSVRNLIKIDFLGALPTELGFKILCYLDTTSLCSAAQVSRRWRQLADDDVVWHKMCEQHIDRKCTKCGWGLPLLDQRRLRHEKRRIQLRASGRGLNEWSPNITPGPETSPVPALIRSASDISDSTTGTKRSAPEDSTSPELSTKRPCTNMEVGQDLASYFDQPKKRPWKEVYKDRFKVGTNWKYGRYTTKIFKGHTNGIMCLQFDDRVLITGSYDATVKVWDIKTGQEIRTLTGHTQGIRCLQFDDSKLITGSLDNTMKVWNWRTGELIRTLPGHSHGVIGLHMADKLLASGSSDNTIIVHDFNNYQRTQLIGHSDWVNAVKIDLPSRTLFSASDDMTVKLWDLDTHRCIKTYEGHVGQVQQVLPLPREFEIDEDDFLANANNDTSDTASLASETSQTLPQPPTSSSQSNEETCFFPNDPTRPSPASYMLTGSLDGTIRLWHVPSGRCVHRFFGHVEGIWSLAADSLRLVSGAEDKTIKIWDPRTGKHERTLTGHTGPVTCVGLSDEMVVSGSEDGSVRVHCFTGGVLNSNNFFSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIG4</accession>
    <name>A0A9W8YIG4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIG4"/>
    <dbReference type="OrthoDB" id="244495at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF27">
        <property type="entry name" value="PHO85 CYCLIN CLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="359"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="51296" checksum="CA67425A5D250354" modified="2023-11-08" version="1">MPSFYPRHNLPRQLLTPPEFVPSYHSGCGTTYQQQNGYTGQPAIRQQDDGYDFADRYGQLAIAMPPMYPQAGTYLSNAPPSLPPASSFYEPAGATILPPMRVLPGHSMAEAVAQQQRAQEYNQRVNQPAKEEKPVGGVSAKLDYDMDVMTDFVCDMALQLITPGRMMPTSFRKWVHQVLCATRLPSATILLSMFYLSNRMPMLIGEPKTDTHLFRLLTIALVLGSKFLDDNTFINRSWSEVSGIRVDELNRLEMEWLNAIDYKLHRDPTEQQGWTSWSDHWKVYQSQATVRAGRSNKLSPIDTSMHRRSLNPNKPLPPLPMQQAYNPPPYEYTPKSAQSSYNLPSYSQYDPWRSANDHSPASAPTTGPTTPEYYGTAGTWAPAEGYSRRTMFGFPPLSQPAPAQSQQPSNYGPATYHTQYNTSVWNQHGVNCGCSYCSQRHPPYFMAPGYGPQAVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YKP5</accession>
    <name>A0A9W8YKP5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000892</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YKP5"/>
    <dbReference type="OrthoDB" id="46529at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039298">
        <property type="entry name" value="ACOT13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660:SF1">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660">
        <property type="entry name" value="THIOESTERASE SUPERFAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4378062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="14788" checksum="588BF892EA8B3ACC" modified="2023-11-08" version="1">MVDLGGSLAVASRGLFATGVSTDLNVTYLSSGGKIGDLIKAEVTCDKCTSPPRHCFLPERVPCIRMLWFSYVLSFTANICTMYLVGKTLAFTSIDFSNAKGEVFARGSHTKYVALAWKDPNNIVEELSPKQSGKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH60</accession>
    <name>A0A9W9CH60_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor 38</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_009710</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PRP38 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH60"/>
    <dbReference type="OrthoDB" id="190958at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005037">
        <property type="entry name" value="PRP38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23142">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 38A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03371">
        <property type="entry name" value="PRP38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="111"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="236"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="243"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4363417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="276" mass="31467" checksum="20EB3AEC80C76921" modified="2023-11-08" version="1">MSKPKYDRADAGALLDERGYSGALIRGQNPALLFEKGVRDRITESYYWKEQCFGLNAATLCDRAADLQFIGGTTGITGKPTPFLCLAFKLLQLVPEKEIILEYLNFRGDEDETDGGETNGHGQVKKEENSDAEGDDDETAEKKELHLNAAGKLGDFKYLRCLAAFYIRLAWEPVEIYKTLEPLLTDYRKIKRRLKDAFTLTYVDQFIDDLLTKDRMCATTLWKLPSRANLEDLEMLDPRESPLGDEVDALDDEDMKKKEQKSWGTQPKEYKEPQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHT5</accession>
    <name>A0A9W9CHT5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Adenine DNA glycosylase</fullName>
            <ecNumber evidence="3 13">3.2.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">N0V83_009033</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Adenine glycosylase active on G-A mispairs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site.</text>
            <dbReference type="EC" id="3.2.2.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="13">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the Nth/MutY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.31" evidence="3 13"/>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHT5"/>
    <dbReference type="OrthoDB" id="10248838at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034039">
        <property type="term" value="F:8-oxo-7,8-dihydroguanine DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035485">
        <property type="term" value="F:adenine/guanine mispair binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032357">
        <property type="term" value="F:oxidized purine DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000701">
        <property type="term" value="F:purine-specific mismatch base pair DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03431">
        <property type="entry name" value="NUDIX_DNA_Glycosylase_C-MutY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000002">
        <property type="entry name" value="Adenine DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044298">
        <property type="entry name" value="MIG/MutY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029119">
        <property type="entry name" value="MutY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42944">
        <property type="entry name" value="ADENINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42944:SF1">
        <property type="entry name" value="ADENINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14815">
        <property type="entry name" value="NUDIX_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="15">
        <location>
            <begin position="117"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="338"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="380"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="338"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4364439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="574" mass="63334" checksum="657FD0279C45DC18" modified="2023-11-08" version="1">MAPRKKPTAIRKATKSVVSRPAKVIKVTKPAITAPTSPQSTALPPSRAHHASYHYPLLSDDKASSDALLAWFEGVEETRSMPWRKRWIDPKDFEGREQELGSVLGKRAYEVWVSEVMLQQTRVSTVIPYFNKWISKWPTVENLAAANHDDVLSVWKGLGYYSRATRLHEGAKAMIAKSFGSSCLLPSDATELQEFPGIGRYTAGAVSSIAFGKAEPVLDGNVARVLSRQLGLYMDVKDKKASDILWEQADQLIKRVSNYTEASTSAIPGQWNQALMELGSTICTPRPRCDECPIQGTCRVYSEGQALSDKRQSPTVVPVVPDIEDACSLCELLDTEDLVTAPEQDDNEEKTRPTKKRKTGTKQANTISQYFLVGTPKSIAETEDGDESGDSASNDPKKRKAPVSAANSKFTSSYCSLFPKKSAKKKVAEEDCVVCLIEMALSDGSSKWLIEQRPAKGLLASLWQFPQCTLAASQSTPANRKTSAQDFVSSLDIGDIDTSKAHYVASFPPLVHVFTHLKLTMHAYHYKITADKAEDVDLACNGPLARKWVDTETMDDETLSTGMRKCWELVEKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI86</accession>
    <name>A0A9W9CI86_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Allantoate permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_009969</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI86"/>
    <dbReference type="OrthoDB" id="1935484at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000247">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000106">
        <property type="entry name" value="MFS transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF29">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="376"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="413"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="442"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="472"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="504"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="540"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4363673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="598" mass="68028" checksum="0FA683F0CB36181F" modified="2023-11-08" version="1">MDDTQRLLPDVQSSFGTFADNMVPAGALDDSIPVALQADTLSIQSIEKSDKPPAAVTRDAQLVTAKGNVITKDGIVFGNHGSSDSSLNDNIFADPEVRDYYKQVYEDAQYECRHIFDAEAAWSKEEERKVIRKLDWHVCLWACTMFFSLQIDRGNIQQAVSSSLLKDLHMTTNDFNYGNTVFLVSFLVAELPSQLISKKLGPDRWIPMQMVLWSIVAMSQAALTGRTSFLVTRALLAVLEGGFIPDLVLWLSYFYTSRELPIRLSFFWTALSVTTIVTSLLAFAIFHLDGIHGLAGWRWLFLLEGLITLLIGFASFFLMPASAVQTKTWFRPNGWFTDRELVIVVNRVLRDDPRKGDMHNRQAITPKRLWSSMKDYDLWPIYALGFICFIPEGPPAYYLTLILRSLGFSTLNVQLLVIPSSVAHIITLLSITWISEKLNQRALVAIWQNLWTLPCVIALYKWDDLINSKWGTYALVTVLLSYPYCHAINVAWTSTNANNVGSRSVSAALYNMFVQLGGIVSANIYRDDDKPLYRGGNRNLVIINILSIGLFIFTKAYYVWRNKTRERKWEAMTREERLHYVRTTADTASRRLDFRFAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIZ5</accession>
    <name>A0A9W9CIZ5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_008673</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIZ5"/>
    <dbReference type="OrthoDB" id="660550at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05471">
        <property type="entry name" value="pepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000060">
        <property type="entry name" value="Aspartic-type endopeptidase ctsD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034164">
        <property type="entry name" value="Pepsin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF75">
        <property type="entry name" value="ENDOPEPTIDASE (CTSD), PUTATIVE (AFU_ORTHOLOGUE AFUA_4G07040)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="13">
        <location>
            <begin position="1"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="273"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="275"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4365057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="348" mass="35787" checksum="A43B67D39A64165A" modified="2023-11-08" version="1">MHTDSTPFNVTYGTGSVSGSLATDTIHIGTLSSSLTFGLATNVSAEFQSYAMDGILGLGRGTTQSDSQPSVMDVLSTSNLISSKLYGIHLSRSKDGKLDGELNLGAVNKDRFTGDLNWSDVVDNDAGFWEIPLSSATVDGNLVSLTTDGARTAIIDTGTSYILLPPADALALHSGIPQFEQSSDKQTFSVPCDTGSTIAFSFGKEVYNISSADWIGAKLDSGLCRSNIIGLQTFGATQWLVGDVFLKNVYSAFDFDNKKVGFGVLSDDDAEEADATASSSSGSSSAKVSQTGTASSGSADPTSSADAASGAQQPEGQKGAGGAAGKATASLLGFAVMIALLSSFVFTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ07</accession>
    <name>A0A9W9CJ07_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008778</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ07"/>
    <dbReference type="OrthoDB" id="10059120at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21456">
        <property type="entry name" value="DLC-like_SpDlc1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.40">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005334">
        <property type="entry name" value="Tctex-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038586">
        <property type="entry name" value="Tctex-1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255:SF4">
        <property type="entry name" value="DYNEIN LIGHT CHAIN TCTEX-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255">
        <property type="entry name" value="T-COMPLEX-ASSOCIATED-TESTIS-EXPRESSED 1/ DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03645">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4365160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="16894" checksum="DC0FCEE56D8CC54E" modified="2023-11-08" version="1">MAVSTTVIDPVVKQVTHHLQSPLPTEELQQIATSACEQAIGAHEAYNHSNVADWNTEIIQSILKTLISKTSSNSTTDPSSTTSEAAQPAYKYIVNSTVIQHIGSPSAPEKHGRRGMHSAVGAFWNNEKDGTYSYKWEGAEKKGMDIVITITWIAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJK1</accession>
    <name>A0A9W9CJK1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">U3 small nucleolar RNA-associated protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_007768</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UTP11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJK1"/>
    <dbReference type="OrthoDB" id="29058at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007144">
        <property type="entry name" value="SSU_processome_Utp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12838">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12838:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03998">
        <property type="entry name" value="Utp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="152"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="190"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4366133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="294" mass="34241" checksum="4B559C57A7D634C1" modified="2023-11-08" version="1">MCRRLSESTLSTPEHPTTLTMSSMRNAVQRRNHKERAQPVERQKWGLLEKRKDYKLRAADHREKKAKLKILSQKARDRNPDEFSFKMMSSKVDKQGKKVADRGNEALSVEVVKLLKTQDAGYIRTMLQMARKEREQLEQRLILEEQGEVKAYKDEEKSRRSQHRVFVDNEEEQEEFDPDAWFGHGQEMPGRVDMDPAHVEDLHAESSQDEDEADAPQIRTLSKKQLEAQELARREARKFRKDRGAGTITDSLQKRERELAAADDELEAQRAKMNSTVGGVNKDGVKFKIRERKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJP8</accession>
    <name>A0A9W9CJP8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">chitinase</fullName>
            <ecNumber evidence="3">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">N0V83_007934</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the glycosyl hydrolase 16 family. CRH1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJP8"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02183">
        <property type="entry name" value="GH16_fungal_CRH1_transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF27">
        <property type="entry name" value="GLYCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040806401" description="chitinase" evidence="14">
        <location>
            <begin position="22"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="15">
        <location>
            <begin position="28"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4366298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="373" mass="40976" checksum="638FFD339D4B77D1" modified="2023-11-08" version="1">MKFLAGSALAVVAALLPTTLAQTHTDCFPLNTTGCPDMSALGGNATFNFNNTGIPDGSKIWEKQNQGTIDWLEDKGATFTVARSGDSPMVQSKFYMLFGRFEVVMRAADGQGIVSSAILQSEDLDEIDWEFLGSNHTHCLTNYYGKGNTTVGDRGKEFKMDAAPQDDFHNYTIDWTKDRIQWWLDGNMLRQLNYSDALGGKNYPQTPMNVRLGIWAGGDTSHNDPGVVEWAGGKTNFDDGPFVMTVQSIYAQDYTSAATYSWADMDSSGSWEKVKVITGKSDVLKEIESPSGVKNRWKALPQGAKIGIIAGAIGAFALAMLALLFCCIKQRRAGRREHAALLAEEEKDAAELQEYKRQMQSGKFGFGSTVHRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJU7</accession>
    <name>A0A9W9CJU7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein SSH4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_007906</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the proteasome-dependent degradation of fructose-1,6-bisphosphatase.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJU7"/>
    <dbReference type="OrthoDB" id="25503at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12909">
        <property type="entry name" value="SPRY_RanBP9_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035782">
        <property type="entry name" value="SPRY_RanBP9/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="3">
        <location>
            <begin position="177"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="CTLH" evidence="4">
        <location>
            <begin position="469"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="133"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="566"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="133"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4366270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="708" mass="78250" checksum="A96429D400F63936" modified="2023-11-08" version="1">MNVFANIVLRDRMCRSITIASAMNARRESWARVAAGSAGSNQSIPAPTRSGAFSHLVNSGSTPHMISQNRHSRSIDADGHHNSMSTSWGRGGPLPSYSSHSGYWQGLGGSGQDIPPFFVPSYLRGSKHAERLQETHKAKLAAQREYKSTHSSNAPSLSTSSSSVNLHKMAPSHRGLSHDIIERAPPFVDEPAAQWPTKWNEGDKFAQLELEDGGRLAKFSGTQKTHDEAAAVRADFPMPRQCGIYYYEITVISKGRDGQVFRPLSVRMPPDNLTRRMIGVGFSGPKVALSRIPGWEPDSYAYHGDDGQVFSNTTSGKAYGPKFGTLDVIGCGLNFRTNTAFFTKNGHMLGTAFRDLKPNMPYYPTVGMKKPGENVRVNFGQEPFTFDIDKMVQDEKAAIQAEIARSKSASNVPADETELIHQLVGQYLAHDGYVETARSFAEEVIEEARALANDEDADISYRAVVEDPHALNRQKIRTAILEGDIDKALKLTTAYYPNVLRDNENIYFKLRCRKFIEMIRRCGELNAECLPVPTPPSKRSTASTVNNRNSTATDEYDFEMELDEQLGVHNPPPSWDNKDQDDDLDDEDDIEDKEAKLQQLTDATITYGMELRAEFANDPRREVKRALEDTFALIAYENARESTLAPLLEIGGRVPVAEELNSAILVSLGKSSSAALERLIAQTEALVTELAADGGPGAFINVRRDFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKB4</accession>
    <name>A0A9W9CKB4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKB4"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="171" mass="17878" checksum="CA0B09EB48DAD380" modified="2023-11-08" version="1">MEDAPIRRAAPGRRQRAALRAGAVPINERQRVFAESVAKGQSNRTDRGDKIDHLVVAVAGTSLALLHGTAKTNGADHEIVPSGGGILPLVVAVTDTSLALLHGTAKTSGVAHLLEQLGGEINHLVGSIGTRHGTARTNGTEDEMEQPGDETNHLVITIIARPPTEQSEGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKG9</accession>
    <name>A0A9W9CKG9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006651</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4066896at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031991">
        <property type="term" value="P:regulation of actomyosin contractile ring contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032955">
        <property type="term" value="P:regulation of division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057454">
        <property type="entry name" value="Bud3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051492">
        <property type="entry name" value="Dynamin-Rho_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032634">
        <property type="entry name" value="Gef2/Nod1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22834:SF21">
        <property type="entry name" value="GUANYL NUCLEOTIDE EXCHANGE FACTOR, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G11890)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22834">
        <property type="entry name" value="NUCLEAR FUSION PROTEIN FUS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17114">
        <property type="entry name" value="Nod1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25351">
        <property type="entry name" value="PH_BUD3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="2">
        <location>
            <begin position="244"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="799"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1082"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1169"/>
            <end position="1412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1480"/>
            <end position="1503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="842"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1098"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1208"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1220"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1330"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1353"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1384"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1486"/>
            <end position="1503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1525" mass="168023" checksum="EEDAB748AD2D4E1C" modified="2023-11-08" version="1">MVAINPEPAALSADDLSLFYTTDELLSNSPVLIFYGPTATSTQATHSRIQTHVFTPAGLQSFARLIISPTATFYNAVTCLPREEQGDEICRGLAFSLYKYFVELPSEIKHAWEKRYSTTGQLPSAPKLFSESHAAIVAAKMVKVENVADVIVDVRHALGEQTLSWIDLDVVLPQGSIQKLETRESAQFDESEDDISQQRYGQYAPIVSLFGDAAFLPTSRLRRAPSKPTALNRSQSFSRKQKETLRREMCELLDTEENYVSKLDELVHTVAVEFREKAKQKSLSSSSPSEEALQALFPPSLDKILEINAKFLDTIRIILEETENGAIEDIEQATDAVFIAPLRGQKDPIDVTGAAAVAKALVEWFPQFGDCYTDYMAAHGDFSQLLKFFTKDTTSSFSKRVYETGEQRLMSMLIEPVQRLPRYNLYIDNIIKQLPVRHPAIKTFLKARDIISEICSREGPTAQQIRIFDRLRKLVSGWPTSFNPQGRLITAIDYVELPPPYHGDLTGPSTVSGIFVLFTDFLVLLNKPVGCTASARSLLADLDNPKFAESFNGSTEVSYHQHLKLCDVFVSEHSEGNILQVLSPTPTSTQPTRPRSRDRQYVGIRMFYLHGAYEGKGSKAVEEFTKARVEGRFPETERESYKWEVRSLAGDLSFFSSVSEETGSPKVEGRKEPAKVQILVEASKHSQSIQVGDEGVELTVNISILGDGFFLLDTNGSNGYTARDKLTNHEFLPVLTKRLANYFQLRNSVRNPTLCETYLFRNQQILKSLSLQLNALEEEQGSKSRPHSPVKMLSSLFGSSVSREGGSRRLHRNPHTLGEIPRMAPPLPPTPARTYSRDGDTSRPTSSNKNAGFSAMAPIDSLAKLEESLANLTLAIHARKGNIVGRSVRARAVADELIVNELYNSLLENPSNLDLAGQSSVDVLFAVFEKFLDVAWKERMGPVLSHAMFVSLQMRSDSMYPGEFEEFFRTTFNDLTPQNQRALRTIIHLLAELLDGTSNDGDRGILTAAFAEMLVPAGNANDFISLMDRLVQDIDALFPIQTPGLNTPNYGSIDSKGRATAAGSINSNTSLRKRFGFGTLTRENSKSESESKVGSLWRSLSKNSHGPDSQPSSLSKAGPASLNRSNSTDAARLSPKRPSSRDRPTVLGAFTFENASTSSRAFLGALGTIGEVPPTAGPPRKKRRSSLSDLKTLQDANDAPMWSSQTPRKPESGLRDSRQTSASPRTPSRTTPSPAKHTSSIPTPTRLGSPMRKENSPGNILDRAGSVARPKSLAAKVEAKEEVTVKSYSPTKRRGESVSGIPSLKPVTSSGLSERPGSGNTIKMPPPGSRSPTKTGTSSASPPKRLRMQSPQKLRERLQNQQRDIDTASEDLQNELSAIGQELSARSTPRLTTQSSAPPSTSGHSPMKSAESRITTLEKHLKASLDALSARTTGIANDVSSSLQVSEARCKHLDQLYRDMNAENEALYARFNEELDKVTSSARKGKASEEVEQRLKASEEEAARLRKENARLKREVAGLKAQIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKI8</accession>
    <name>A0A9W9CKI8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial import inner membrane translocase subunit TIM54</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_006885</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIM54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKI8"/>
    <dbReference type="OrthoDB" id="5598305at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050187">
        <property type="entry name" value="Lipid_Phosphate_FormReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021056">
        <property type="entry name" value="Mt_import_IM_translocase_Tim54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358:SF101">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358">
        <property type="entry name" value="SPHINGOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11711">
        <property type="entry name" value="Tim54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="242"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="363"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="267"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4367304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="467" mass="52854" checksum="F335C6CB1F9AD95C" modified="2023-11-08" version="1">MADTDAKAAPASGASAGPSKPQKPPNPVWRMMGLPNFRFKLPSRNWMIFLSITGSWTAAVMYDRREKKRIQKKWARVVEHIAKEPLDTQQLQRKLTVFVSAPPADGLVPARDHFHEYIKPILVAAALDWDAVEGRREGDIRAGLAERIRKLRKKRGETSEEPIEEGTEELLEATRQRAGVREWDGPAGDIVIGRHTWKEYVRGLHEGWLGPLDAPTLQAEAIEPPAETVLPAPGATPELLKTEPAEETTAPPVATDDTSPTAAPEAPKTDEAEKPKEEENKPKKKKQPPPFITTAEYENATLSRNCPQALGPTAIIPFPHLLGFFNFPIRMYRFLNRRQAADVIGRETAAAVLGAYRPFETSGEASTFNDQDDHSSTPWEQQKLLKHEEPDWHKSARKREEDDKKERVWLDDMVLDPRIAERMRRFALDAEVEERAKNLPRAEEKSWFSSWWPQEKKKGAWEGLSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKJ5</accession>
    <name>A0A9W9CKJ5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007588</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKJ5"/>
    <dbReference type="OrthoDB" id="3694718at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="255" mass="29017" checksum="8C9538339042891D" modified="2023-11-08" version="1">MFNPWRQDPFQRLPFTHPSFSSYQKHSAQNYKQQHRPRPHTPRRARHSSLFNLDAIEGSDFSSDETSLSERYYLSDDHHHHNTFPSLFSKSPEDTSDHRRATIKPALHNLKLNIPKSSLETLGLREDDVLILAPGTTLTRLKVQIRDHHNHHHHATTETLRAVAVAADVRGRDIIKQLLPGLSGEVRAYVRTRGSEWQELDLGAKVGSVVAEMGGSVVELRIVVSSGSKGLERRMGVGMAWQKEMGVGKEMFWMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM21</accession>
    <name>A0A9W9CM21_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="16">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">PPT1</name>
        <name evidence="18" type="ORF">N0V83_005403</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Protein phosphatase that specifically binds to and dephosphorylates the molecular chaperone Hsp90. Dephosphorylation positively regulates the Hsp90 chaperone machinery.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:20630"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:47005"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="16"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM21"/>
    <dbReference type="OrthoDB" id="445564at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07417">
        <property type="entry name" value="MPP_PP5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000039">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041753">
        <property type="entry name" value="PP5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013235">
        <property type="entry name" value="PPP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051134">
        <property type="entry name" value="PPP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668:SF5">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08321">
        <property type="entry name" value="PPP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033096">
        <property type="entry name" value="PPPtase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 15" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="15">
        <location>
            <begin position="42"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="17">
        <location>
            <begin position="278"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="14">
        <location>
            <position position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR033096-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4369641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="477" mass="54453" checksum="47EE79B556C74288" modified="2023-11-08" version="1">MGNPLEEATQYKNQGNDAFRNQAWDNALEFYTKAIEAYNREPSFYTNRAQTYIKLEQYGYAIQDADTAIELDPNNVKAFYRRASANTAILKHREALRDWKLVVKKAPNDAQAKLRMAECEKVVKRDAFLKAIEVDDAPSAAEGLDIEHMAVDSSYDGAKLGDKMTLEFVEDMIERFKNGKKLAKKYVYQIIVAVMDIVKKEPTMVEHDVEEGHEITVCGDTHGQFFDLMEIFRLAGKPSEKHSFLFNGDFVDRGSWSTEIALLLYAYKWLYPDKFFLNRGNHETDDMNRMYGFEGECRAKYTERVFKLFSESFSMLPLATLIGKKYFVLHGGLFSDDKVTLDDVRKLDRFKQRQPGQAGLMMEMLWTDPQTAPGRGPSKRGVGLQFGPDVTKRFCENNGLEAIIRSHEVRMDGYEVEHDGRCITVFSAPNYCDSTHNKGAFIKIGPEYKLKFTQFDAVPHPNIKPMAYASNGMAGMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM70</accession>
    <name>A0A9W9CM70_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GTPase-activating protein GYP7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">GAP for YPT7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">GYP7</name>
        <name evidence="6" type="ORF">N0V83_005555</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM70"/>
    <dbReference type="OrthoDB" id="10264062at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000032">
        <property type="entry name" value="GTPase activating protein (Gyp7)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000005">
        <property type="entry name" value="TBC1 domain family member 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF502">
        <property type="entry name" value="SMALL G PROTEIN SIGNALING MODULATOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="5">
        <location>
            <begin position="431"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="239"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="727"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="247"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="263"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="733"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="757"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00072091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00082648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4369791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="814" mass="92185" checksum="BF931975F5D61046" modified="2023-11-08" version="1">MASPARTPPSPSASFYDLSDDEEGEYNTIRHTSSGKGVKLLYTKSKVYVHPSPSTKDNIPGFVALVQQKSARSTNDARPTSSSSARSVNASSLLLAWIPESGLGDAYDTYVKVDLSDSSSPPRQSYLVPPPPTTSTHSGTPGYAFAIPVSEIYSVLVRPPSIGWWFGSVVVNTRAGDSFPALFFHDSECQSTILQRKKLAKESFDPFGDGGGMFWGGDEVLRWLKRYVTVERSGADPSVYLIDPSEDDKRSFGKDVAPRKSTEGSGNASSSQPPGKRDGGMDPVTKALKEARWSFLEKLSQVTTFTRRTAQAVAENPKIPPQVRRLIQNPEVQTLQEEFDSARLYLARWAMGIAEQSERDRNQRIWTAKDVLAMEQSDVGDFEILDMDKMTMADRRKPVTLTEWKGFFDPKGRLQLTPDEVKDRIFHGGLDPDDGVRKEAWLFLLGVYEWDSSEEERRANVNSRRDEYIRLKGAWWERMVEGHQDEEQEEWWKEQKNRIEKDVHRTDRNIPIFAGEDIPHPDPDSPFSDVGTNVHLEQMKDMLLTYNEYNKDLGYVQGMSDLLAPIYAVMQDDAVAFWGFVGFMDRMERNFLRDQSGMRKQLLTLDHLVQLMDPKLYLHLQSADSTNFFFFFRMLLVWYKREFEWADVLRLWESLWTDYQSSNFHIFIALAILEKHRDVIMAHLKHFDEVLKYVNELSGTMDLESTLVRAESLFKRFQRTVETIDKKGNFPSAPQARQRKQAAAIVAGPGPKLDPRPSSSNAEAATSGTDKGKQVGEVDEDARVISPELRALLSRQVEKLDKSTVVEHGGGVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMQ7</accession>
    <name>A0A9W9CMQ7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosome biogenesis regulatory protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RRS1</name>
        <name evidence="7" type="ORF">N0V83_005229</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in ribosomal large subunit assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the RRS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMQ7"/>
    <dbReference type="OrthoDB" id="28455at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007023">
        <property type="entry name" value="Ribosom_reg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04939">
        <property type="entry name" value="RRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="189"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="189"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4370708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="235" mass="25706" checksum="07DEB1CFB472C4CD" modified="2023-11-08" version="1">MSDTEMNGGIALDGPSPTHSTLADKITGGAMDVKSSHLNGALSVADSTVSSRLPITVNKPTPFTFDLGNLLANDPNPLEADADEQVLSATARDAAQALINQLLSTCQIQSTSEGVHLVLPAPSTPLPREKPVPAAKEPTKWERFAAKKGIKDKKRDGKMVYDDTTGEWVPKWGYKGKNKEGDDQWLVEVDEKKEKETGEAHDQRADGRRERKERIRRNERKQRANDAKSRKPGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMS9</accession>
    <name>A0A9W9CMS9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_005230</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMS9"/>
    <dbReference type="OrthoDB" id="2962993at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000034">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF19">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G01812)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="335"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="434"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="65"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4370709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="491" mass="54982" checksum="00B97805E8B34214" modified="2023-11-08" version="1">MAAAVERHSDSPVSEEKRIGDEHLEKNEIGHRHTIENLPDPDAGLSDEERAAHDRKLLRKLDLKLIPWLSFLYLISFLDRTNIGNAKVDGLQEDLNMTNGQYNESLTIFFVSYSVFEPLTNVLLKKLRPSVFLPIIMIWWGICMACMGLVHNYAGLMTARWWLGVAEAGLFPGVNYYLSCWYRRSEFGIRAAVFFSAAALAGSFGGLLAAGIAQMGGVGGKPGWAWIFILEGLATIFVGIASYWMVHDFPAEATFLTPDDRARVLRRLRADKQASAAHEDFQMKYFWQSVKDWKTLMFSIIYMGADGSLYAFSLFLPTILQQLGYTSTRANLLSVPPYAVAAVCTVLVGWIADRTKQRGLCNIIMALFGIIGFSMLLGSGTPGVQYAGTFLGAIGIYPLHREHHLLGIQQPGRRLQAWRLARDKPRFKLGHGVVLAYLTLFLFGGSIVTRILLAWENKKRKSGARDTWVEGKSEEEIHEMGDGRPDFIYTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMT2</accession>
    <name>A0A9W9CMT2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bem46 protein, variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">bem46</name>
        <name evidence="3" type="ORF">N0V83_005051</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMT2"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF81">
        <property type="entry name" value="PROTEIN ABHD13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="2">
        <location>
            <begin position="91"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="296" mass="33178" checksum="31BCD94AACDD1E0F" modified="2023-11-08" version="1">MASALETFMSYLRLPLLASSGVAALLSGLLYFKQNEIIYPRGFPPDARTNVPRPSQFGITDFEELFIPTPDGESLSAFFIRANKQHARNVTILMFHGNAGNIGYRLPIAKILENQLRCNVLMLQYRGYGLSSGNPNEKGLMIDAQTGLEYIRQRHELRGTKIVVYGQSIGGAVAIALAARNQKHGDIAAIILENTFTSIRKLIPTAFPPARFLAPLCHQIWPSEETLPQITNIPILFLSGLKDEIIPPSHMAKLFQVCKTFNKVWKELPNGSHNDTVAEPRYFQYIEEFLSEFVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMY6</accession>
    <name>A0A9W9CMY6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_003809</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMY6"/>
    <dbReference type="OrthoDB" id="762982at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF49">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="34"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="57"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="754"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="135"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="754"/>
            <end position="766"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4372036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="886" mass="98150" checksum="8C348D53FA4F46CF" modified="2023-11-08" version="1">MPPTPPSTTSSSAGHSPPDPQFRVVRKRNRVPLSCGPCRHRKLKCNRGHPCDNCTKRGDTASCTYATPGNRKKSTASTGPTTSPDDMQNRIDRLEGLVLSLMTNGAQSAGPAAAQAAIAASLNSSSSDQQFDISNMSSGMSNNGAPESIPEEDVEEESEMENVTKSIGVMKVNNNHTVFASEAHWYAILGELSEVKNYFAEHKKQYDDQLKRYKAAHSEERPPGTSFLMGGQKPSSKAEVLSRFPPKPTADILVSRFFNTYDPGIHIIHGPTFQKEYDRHWLNPDETPCIWLGLVYSMMCIALQSYTRAGDEPPEYHNKSWSMSKEYGDLTAQCLIMADITQPITGMLETLILHINAEYARSRDAEVGILISTGIIVRLAMRMGLHRDPGPYPGIPVFQGEMRRRVWAAVRSLDLLFSAQAGLPPIVRPRDTNTGIPRNIYDDELYEDVKVLPPSRPETEATPTLFLINRTRLVYKLGEAVELADTLTCASYDEVMKMDSQARELHDNISPHLKMRTMDESARDPSTLIMQRFTLDLLYLKIMCVLHRKFLAYARVQPRFSYSRTAVIDASMTLLQHQATLHRECQPGGRLRNVKWFISSLTTVDFLLAAMIVSSDLYHTIRIERQGRSPPAEMYMWSSDRLDEMLEAIETAVHIWDGLKDHSMEAYKAHATLSVMLHQLKKDRPARQAPNGFQAASSAFPTAAPMDDANVAPEHSAAMTLGMLSTGGMTPNSANMFDQRYPASMANLLNDPVPQQSTGLTPTYNGGTSGPAENAPSPFSNLFGANLFQNLDLPPTDNINWDAWDNYIQGPGIDTSNNFFPMDLSGMPMQTEQANAVPNMEPTPAQQAAQNTRTNAFGQNVFMGVDPKTASGEGYAMGFLLPKRDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNP5</accession>
    <name>A0A9W9CNP5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_003475</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNP5"/>
    <dbReference type="OrthoDB" id="951172at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000313">
        <property type="entry name" value="Importin beta-2 subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000219">
        <property type="entry name" value="Importin subunit beta-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="7">
        <location>
            <begin position="32"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="351"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="383"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4373183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="943" mass="104460" checksum="008676CBFCB7BB22" modified="2023-11-08" version="1">MAWQPDEEPLRQLTQCLRDSLSGHDQNARNNAGIMLKSAKDSPDIDKYLAYVFSNSQPPPAVNMDAGGYFQARAAAAVMLKNDVKTAYKDMPESTKDYIRSIILVGLQDPSSQIRGYAGNVITEIVRQGGIMGWPQILSELINMVSNANGSVSTQAQEGGMSALLKICEDNRRALDKQYQGQKPLNFIFPKLLELTTSPQARVRADALASINIFVPENPEAVISNIDALLQQLFQLASDPSEDVRKHVCRAFVHIADIAPQKIVPHMEGLVDYMVTQQRNDDNAELALDAAEFWLCVGEDEKMRQHLGPYLPKIVPVLLSSMVYSEDEIVRLEGEEEDYQVEDREQDIKPTFASSKAGRLTTGANGEDANNVNGAPGSSANSIDDDLSEGEIDEFDDDDDEFGEDPEQQWNLRKCSAAALDVLASVFHEGVFQATLSYLNNNLDHPEWPHRESAVLALGAIADGCMEVVEPHLHKLTPYLITLLQDPKPVVRTITCWTLGRYSGWAAHLDTAGKRQFFEPVMDGILKKMLDPNKKVQEAAASAFANLEEKANTELTGYCGAIVHQFVQCFAMYKDRNMFILYDCVQTLAEHVGPALAREDLVSILMPALLQRWNKVSDQSREMFPLLECLSYVTTALGRMFSQYAGGIFARCVKIIHRNLEDGVFAVENPGFEAPDKDFLVTSLDLLSAIVQALPEGDSAQLVAGTPSFFQLLAVCMRDTNNEVRQSAYALLGDCAIYVYGQLQPCLPTILEILIAQLEVRQINLDGETGYSVINNACWSVGEISMRQKEAMQPYAERLLEKLGSILFDEKVPESLNENAAIALGRLGLGCAQYLSVHLAQIAPAFLRAIKKVTWLDEKLHALTGFMQIMLANPGAMEQSLLEFFNEMAQANSKIVESPLGQPFAQTFQKVIQQYKGMISDFDGFLGALPAEQQSNLHKLYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNT9</accession>
    <name>A0A9W9CNT9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003627</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNT9"/>
    <dbReference type="OrthoDB" id="3786931at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="415"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="499" mass="55493" checksum="E61486FF08AF9236" modified="2023-11-08" version="1">MPLVPKKMETRRLHNSDMKKLVRILRGTPYPFSSSKDKAYRQNAKESIHSLPRRLRSSHLSFSSSTTTSNLCSTHKGLDANLIAEIYAWLRHELDGAIGKFLYPLIMSGVLTHTQELKMRQLEPARQMWQHDFQADLSAPPGRQAIQCGDKWSYQVDQCAACILARIGSDEGVLFALFAGMVGRMKDKASGMDVLRGIVGMEKVKSKRLRFVRYWVKAASRGGENAVFEAGELGLKMKGLRHEWKVQQRRLRDGKNLQPAPKPNMPRQSHETESAGRLFTDAESVIGIDITVPYQPKEWSPLNAHDPKLGPNPRPSNAIPLSPAALLGFGIPGFRDSDPHSPHAESESKPPLARPPSTSSDTTIHPGSSISVAHLRPPPLRPQKPPKMQEALVEKYRAMLAAHSNAYASHPIYEEEEDGDDDASSGNDLPKPRPTSMYESFGNTGFDDDKFEGVDEEVEEIEEEDGEDVGEWRAPQTPNVATYRRPSVASASTGWDGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNU7</accession>
    <name>A0A9W9CNU7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cystathionine beta-synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_004187</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNU7"/>
    <dbReference type="OrthoDB" id="2536440at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42115">
        <property type="entry name" value="BETA-SYNTHASE (BETA-THIONASE), PUTATIVE (AFU_ORTHOLOGUE AFUA_3G08420)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42115:SF1">
        <property type="entry name" value="BETA-SYNTHASE (BETA-THIONASE), PUTATIVE (AFU_ORTHOLOGUE AFUA_3G08420)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4372413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="17940" checksum="71102BC6ED1D03B6" modified="2023-11-08" version="1">MATEAPTSNGDYQSLWANKYRGATVEDLDPPPALSVKPSDPISWALMSGLERDYTHLTVISQENRALLGYISIPHLQQLLKEGKVNDSDPVEEAMHKFQRRGRRYKVITTETPLEELEEFFAGGVDGSGKQEFAVVTDASRKFVLGVATRGDLENFAKRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNW6</accession>
    <name>A0A9W9CNW6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNW6"/>
    <dbReference type="OrthoDB" id="3641178at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="320" mass="35627" checksum="1F5287836D0F7CEF" modified="2023-11-08" version="1">MTRYNEMKEKTQISKALYQSRHYTQCAKFNEQLLSEVHNDVSARDDIHDSNLASRASDAYNASQPTITDGYQVHPLHVAYLNFYTALSHDTLAREATLKNRYRELSLAEKHYSAAIAVLSLSHSPKSEDEQLSSPVSTTFNEDQTWTRRSSNAGSFDSTTSAASSATSYSPEILDLIPELTPKRLARSQFSQPARSFGSDATAISKILKRNDSINAPALTQTRTPQGYQFAADTSAFVRMMEAHLASVKQLKQKTAIPGVRFTFPSPPISPATSKPRNSHLFDNATAEAVRQKRRTVTFRPRFDPSSVQRLCTEALAEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP67</accession>
    <name>A0A9W9CP67_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fumarylacetoacetate hydrolase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_003326</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP67"/>
    <dbReference type="OrthoDB" id="194468at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018773">
        <property type="term" value="F:acetylpyruvate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050163">
        <property type="term" value="F:oxaloacetate tautomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.850.10:FF:000002">
        <property type="entry name" value="2-hydroxyhepta-2,4-diene-1,7-dioate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820">
        <property type="entry name" value="ACYLPYRUVASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820:SF7">
        <property type="entry name" value="ACYLPYRUVASE FAHD1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="4">
        <location>
            <begin position="88"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="352"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4373035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="597" mass="63670" checksum="EFEA8B27CD9C674F" modified="2023-11-08" version="1">MSSVGSANGQMPFTNFVSYQDAQSDRNRVGHYDLTKKTIQPLSFTSGTPIETLYQVIEVGESNIISAGGPPVPDSSIKILPPFTGRDILCVGKNYAEHAKEFNSSGFDSSDKVDTPSHPVIFTKRFTSIIATGEEIFPHPEFTQTVDYEGEIGVVIGKPGFRVKEEEAMGYVWGYTIVNDVTARERQRDHKQFYVGKSPDTFCPMGPIAVPASKLDKVLRVQTHVNGELRQNATTDDLIFSIPYLIKTMSEGQTLMPGDVLATGTPAGVGIGRKPPIYLKPGDEISVSVTGLGTLTNRIADPGSHNPVVQQVQNISHLRQTNATKSIDPSSLTQINNKPLFYKHLGDSKASPIVFIHGLGGSAEFYTPLIQALALEKSHSLHLFDLEGHGLSPTSPLSRLSIDSFAADVNGIFENGGITSSEGATLIAHSMGCLVAVAFALAHPEKVAKLILIGPPPSPLPEAGKNATYARAETARTKGMVSIVDALVAAGTSEKTKTSNQLAVAAVRMSLLSQDPEGYAKACTALAGATGLDFGGVQAETLIVTGSEDKVSPPGLCEKYVEVLKGKATLMVLENVGHWHVFEDLQGVASAVKEFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPN5</accession>
    <name>A0A9W9CPN5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">amidase</fullName>
            <ecNumber evidence="3">3.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_003692</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a monocarboxylic acid amide + H2O = a monocarboxylate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:12020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35757"/>
            <dbReference type="ChEBI" id="CHEBI:83628"/>
            <dbReference type="EC" id="3.5.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.4" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPN5"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072:SF6">
        <property type="entry name" value="AMIDASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G14530)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072">
        <property type="entry name" value="AMIDASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="8">
        <location>
            <begin position="87"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="494"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="5">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="239"/>
            <end position="242"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4373397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="515" mass="56341" checksum="75503F6F8CD7695C" modified="2023-11-08" version="1">MSKSTTQSEPQLPPKWQLIAWQKKDEQYARIPSEWRLDSLPAPNITNYADIPRQCGLLSKEELEITEQYDATGLADAIRGRKLKCVDVTRAFCKRAAIAQQLTNCLTEIFFNDALKRAEELDAHLASGKQPLGPLHGVPVSLKDTFKVRGYDASIGLASLAFKPAKENSVLVDCLLNAGAVLYCKTNIPQTLMALDSHNNLFGRTINPLNTAVTAGGSSGGEGALLAIRGSILGVGTDVGGSIRIPAMCDGTYGIKPSWERLPYAGQEGGALPGASKIGIPASAGPLAHSMRDIELFFSAVAQQKPWLVDPDTVPLPWTSASNGKRKLRIGVVRRDGVIDPHPPIVRILDEVKTKLQKANVDVVEMDITALFSQCQSLANAMFGVEGGNAMFDLLESANEPLSPWLATRLKRKQPVELPKLQQLHGRREQLRKEFLKVWEDQHGEIDAFICPVAPHPVPPIDRYNGVSYTSSFVLLDYPAGVVPVRTFTKEDMHGEMPKTKPLGSWDKANRELCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPT8</accession>
    <name>A0A9W9CPT8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_002751</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPT8"/>
    <dbReference type="OrthoDB" id="438641at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050869">
        <property type="entry name" value="H3K4_H4K5_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197:SF273">
        <property type="entry name" value="MYND-TYPE ZINC FINGER PROTEIN SAMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4374012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="559" mass="63919" checksum="F0BA753B121A4549" modified="2023-11-08" version="1">MQNLDNLALRKRLTQELIALRQHTPEQPYSIAHRLKLAKAYRSLGYPDLAIGDAYKALLLVDEVREESEYHDEALEAAEADFLTYRMAKLDVEQDVNENVAKEHEVWNWAQTKSKTAYDILIRCLIDCGCLRSAFDYITRAKKAFPGDTTFEIYNEVLSKQVDSVTDVDDYPDKGLVRREQYAWNDHEPNRFSEECLTFLNEELRSIAPKLEVKVAELPILSFGGGTNESHTEIRYAKQLGLFAKENISPGVRVLKEKSLLTAIARLHDSYCDACSIPLPNSQDTESAEGAEINIVACEDCDEVFFCSADCHDLAQDSYHPSLCGVDHAQSQVPAREAADYLYSRLLVRALALAETQDVHPLELKEVRYIWGDYHSLDLDSVWQAETSGRLIDAFGSVPQTLPFSFKYNVLVPQQILEKMDVNIFTQSHRYETWIFNTLYAKFRGTASARQGLDGRPEISAVHPMWCLANHSCDPNVGWDWRGTMRFWTREKFVDWEGRDPTKTPGLKKGEEVYGHYCDVRLPVKERREWAVGALGGDCMCERCVWEEAKERQRNDTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQH1</accession>
    <name>A0A9W9CQH1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Suppressor of Profilin deletion</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SYP1</name>
        <name evidence="6" type="ORF">N0V83_002086</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQH1"/>
    <dbReference type="OrthoDB" id="331602at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032185">
        <property type="term" value="P:septin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09264">
        <property type="entry name" value="AP_Syp1_MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07650">
        <property type="entry name" value="F-BAR_Syp1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000102">
        <property type="entry name" value="WGS project CABT00000000 data, contig 2.23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018808">
        <property type="entry name" value="Muniscin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049609">
        <property type="entry name" value="Syp1-like_MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF54">
        <property type="entry name" value="SUPPRESSOR OF YEAST PROFILIN DELETION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10291">
        <property type="entry name" value="muHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="5">
        <location>
            <begin position="6"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="MHD" evidence="4">
        <location>
            <begin position="613"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="238"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="533"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="852"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="339"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="359"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="376"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="397"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="427"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="544"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="558"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="591"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="855"/>
            <end position="864"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4375007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="892" mass="96263" checksum="F0AFE19EF109E16A" modified="2023-11-08" version="1">MELQRKEYPALLTSLTPAQAVNILNDRVNHVSRLNNHIADWLQERRRVEEQYVQGLRKLANRHPPDESSDLGIFSTPWQKIVSATESVAQSHHHLATKIEADVERPLREFAANSREVQAMGNIAGNLAAIAKDIDSAQKKTSKLKDKGTKAKPSAVADAIQDIEKAELQWDSQAPYVFEQLQAIDETRLNHLRDALTQFQTHEVDQVERSRVTAEETLNALLNIETADEIQTWALRMRSGDLPQPSRKLSSTGTPSRSLAPPVPTTPGAADDSRSQKSGSIQEKHSSNPLKRFGTVLGRRRQSQHPYGRASSPERKSSSNLGSAFSGFGKGKSKDRDALGSSLGSSMGSSSERPRSPLRRLSSAARASQRSDSPTHTRLPSTDAPNGTIAESPMEPSDSNAINGTAPVQESIPELKESLSPPPVAETKPEPEKDAEGIFSGLYATSTYLEYPLTDFDSAQSESNPTPQFKLDIRNAPIQEEGEDADAATANLVNTLRAQAAQPKRASTLRGRRDVRNTIFVPNPATPELQGLQSIGEVPPPLPSAGSGPASAAGSATFPPPTLPPQPASPAFKAPHRSLLADEHAASDTQSIRSGRSLSSSASATVRHPDLHGPGLNASLVETVSAWFEQGTVTKAMVIGQVALAFNPIDISAGPFGTESIRLENFPVLEKVAPNPAFIEQSPDNPGNYTVDLARITKTSVAFHYQLHIEDSNITSLAPIILSPVWKPEATQTSAILHYSLNPKFNVGSATSITIHNLVLVMRLEPGSKAKSCQSKPSGTFSRDKGLIYWRLGDVTFFKDQVAQTIRVRFLTEGEAKPGNVEARWEISDEQSLTLGSGLGVSMSAPLNEVKQDGEADPFADTDENAPPASPAIGWKPVTSVKKIISGTYMGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQS8</accession>
    <name>A0A9W9CQS8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ubiquitin C-terminal hydrolase Ubp14</fullName>
            <ecNumber evidence="5">3.4.19.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ubp14_2</name>
        <name evidence="5" type="ORF">N0V83_002273</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYBBP1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQS8"/>
    <dbReference type="OrthoDB" id="342531at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000182">
        <property type="term" value="F:rDNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007015">
        <property type="entry name" value="DNA_pol_V/MYBBP1A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13213:SF2">
        <property type="entry name" value="MYB-BINDING PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13213">
        <property type="entry name" value="MYB-BINDING PROTEIN 1A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04931">
        <property type="entry name" value="DNA_pol_phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="725"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="799"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="726"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="755"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="773"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="810"/>
            <end position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4375189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1016" mass="113632" checksum="B6D59181C1CDD392" modified="2023-11-08" version="1">MGSKIRKRGPDSSLGDDVNEAPAKRRRQINESHLKLSKLYEDLAAESDDVRIEAAKQLIVKFSPENKPAAKDVEATLSRLIKGLCSQRKAARVGFSLTLTELLRQIFGSGKNAIEGLELDVAGIVKMVEEKTKVEGNVPGRERRDHLIGKLFGYKAIMQSSIVIEPKLSLECWNELLDHIYGMARDIPWLREECGMVLVEAVRSLKARAQYQKCAEEVIRRLCAFKLVSTPEGVAVWLTVQAIYEDALPDGIWHVKDPLSKRERTRLAKILKENFQGETEDGTDRATKTASANPNPSFTWDLVLSEILRRDEQSRSDAKEASEFPQFWVDAIDSNLFSSTSSHERKAWGFKLLGNMITRVPDWAVPALFSPNLMRTIINQSKKEDRFLHSAALTSLQAVLARVQRDSSTAFPIFVALTSKNGNVEFDRITKTKTLEHVLISADDESLRRAVRHLNSLVLRPDSEDQTIADSRRHVIADMLLNIVKQYQRYDELVEDVAEKDNWLRRALEILVEYAYFVPSHGAKTSKVPLPPISERTRTIFQERLSSCLTKLFDVNVSSRSAFALMVVSMIRNKSKSSKSLDPFFKADKSVTKTIEEASLTLDAISAQGSIAGNKMAAEGFLLLYSLTLLQAYNGEGDAVMLLDDLDASRAAMLKKEKGSTVEGQDVFVEIILSFLGNPRTLFRRIGEEAFSIFASEIGADGLRSLTDILDTEETLEGQKELFNQADDDAEQDQSSDESEDESDVEMIDGQSQNSGDDLPDASASDSSTSESGSDENDDDDSSEDDAELTQFNNLLALTLQTSKPNLNGDDAEETSDESDMDDEQMMALDPHLSQIFRQRSKTTSKKKQREEAKQNVVQFKSRVLDLLAIYLEKQYSNPLILDMLLAMLRRTRANANKQTGDKAAKILKTFFDTRTKHKAPLPKPEEVEAVWEILRDIHEEAKMGGGAKLHAEACSSASLYVVKVLVGLDKSNYAGVVDVYAGTQKGWFADKKSRLQPVLFTQFQNWSLNARQQGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQY5</accession>
    <name>A0A9W9CQY5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQY5"/>
    <dbReference type="OrthoDB" id="5410741at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="15517" checksum="3705DA93454AAC3D" modified="2023-11-08" version="1">MRLTLTQFHRLKVGSLREEGHRDDPENIKEITPLKGVRFHIAAWVSWWGKAERLRFYNDEEDKVEEPTYPQKPRRRPTTEDKEEYTERDHPAQSPDLNPIEGTWAIVKQRLCRRIFDSEEEMKEALQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRA3</accession>
    <name>A0A9W9CRA3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRA3"/>
    <dbReference type="OrthoDB" id="3772124at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="148" mass="16800" checksum="113A887A0B24D4C1" modified="2023-11-08" version="1">MDRYPQVKVSERECPHEDFSSSRHDSGSSINKAALQRSLDTNSHNQDSSALPQYTSFPPPSNHSQQRTDSTNSSKSSPDEETDQRIDAEFARYTDAPPPYSEKQYEGKSEQETSNMRMKDYAKEISRMMGRQLVRGLKTGKSDKSESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRC8</accession>
    <name>A0A9W9CRC8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">M protein repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001431</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRC8"/>
    <dbReference type="OrthoDB" id="5413982at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43941">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43941:SF1">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="260"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="453"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="374"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="139"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="190"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="210"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="269"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="296"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="453"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="465"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="490"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="521"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4376150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="594" mass="66074" checksum="B470B91C04631C86" modified="2023-11-08" version="1">MADEDKEKAEKVAAAKKRVEAMKKQKAKKAGGAKKKDTKTEAPAEAEASSTPAKSEDKPEDKPEETAPEPVEATSASPGPVEDDDEPSDLPVPKGSHGRKLSMAVESRQRSESFYRSGTAGTPISPGPLSPGGGVTSEVYREQAQRIEELEKENKRLVGEVEDSEKKRRKGEEELEELREGRGDVALAVEKGKEADKLKSEVESLKRQLSQAQTQSTKSTRRTSTASPSQTASIDDLNAQVASKSATIESLELEISNVNRQLSEETNKNNELQSRVSSLETALQKAEQEASSTKTELSELKANLDKASEQADKEGSDRDSAQTRVAQLEAELGAANRKASDSVSRAELLEKKIETLTQLHRDNDSRNQTRLQEHKKVEREAAELRTRITGLSNENARLREAEQRRRKADLGSIEDSSVQELLDEERNKLLAQVRTLEEENFELRRGVWRDRRRDMQPNIDDQHDATQPYSSPRNNSSGFDEIDLSGHAPSRQQLPNRSHSTFTDVLNSGISAFTGQTPSHRRSDAASKPKPRQESLGSLDEFEIDEDAFRLAQEEEGKRRIERVREVKRGLVNFKGWRADFVDVRVGMGGVFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRE6</accession>
    <name>A0A9W9CRE6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001612</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRE6"/>
    <dbReference type="OrthoDB" id="3226064at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="4"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="462" mass="52933" checksum="88EEA5418399C54F" modified="2023-11-08" version="1">MTELLDLCYDVLIRILEEINPEDLAACAQTSWGFNNFIKTNTRLYKAHYLRNFDDPRRRPADPDPDWVEDLQKLTRCQKMLESANNDLKRDEFKFVATTVESLVATGSTDAFGHSHNRDLITKLFRHISQNHDAFMCRSSLFGRVGKPNQKPADDKEGRQLSAKLHCLFGIPSTNAGRRVLSTHPHARSRVYDLRNYTDKTEWGPFRDDGSMRVDWEMIESIMIVLGYNSGLCCRRFLQRFRPPWTEPLEGVIPERAKILPEYPPKLLLEPDIPLHLKDPYNVSGIWSRVVCFLDYNDLYNFNFSAEAMKVPSDELRETITTEEAIRHIMMDLQVTDVQAPGEFDNPSLPIVHFSGKSKSVDAAWDPNANSKLRGTVRLTPDGEVRWQTISVFYGGEERWRSEGVQVGGLRSERGVIGTWFDKDYDPHGPAGPTAFWKICDRHVCGESEESDSEEGDGDWQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRH8</accession>
    <name>A0A9W9CRH8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Potassium transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">KCH1</name>
        <name evidence="3" type="ORF">N0V83_000865</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRH8"/>
    <dbReference type="OrthoDB" id="436496at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031606">
        <property type="entry name" value="Kch1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36424:SF1">
        <property type="entry name" value="LOW AFFINITY K(+) TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36424">
        <property type="entry name" value="PHEROMONE-REGULATED MEMBRANE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16944">
        <property type="entry name" value="KCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="226"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="357"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4378035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="612" mass="67817" checksum="4B5524D6C82F22B7" modified="2023-11-08" version="1">MGCCGDREKAVTVTEEQKWTYINLSDFKSTSCLAPFSYVWLWILVIVSCAVYAADAFTAVNLLAFDKWSSQVKPKVPFEVAKWIFAITIIISYVFLLYRWIMALRVMRSGSVTECYLEPLAAILQSMRLTKSGQGWRRFLVFAALTKSKKGANYIALFVYFQFKSALLIVVAQGPRIAINGMTLYAVMQAQLLPVGDHASEDRSGFDQFFFNIKVMVDTGNKQETVIYFTMLFSLVIWVIAALGLLISGLLYIFFLWHYIPRADGSLTNYCRRKVETRLERIVSKTVKKAIEKQNQQRKRDEQKAIKKGALDPSQTRPTLPKLGGEDDDTSTVFSLQRSDTMSTNTTLPPYSSNAPSRTNTMNTMSTNRNMSKPSLPSLSERPDMPSRSNTQFSTTSYGSNAPLLNQAGGMGMASPVPPMPPLDRNGDYFGDQPPRPYGSAGRPYTPMSQGRASPLPPRGHLPPVDTSYSNGRYESDPQLISPLPSDLRGPPSAQSSRRPYPEFSPFDSRGPQIGPAYELSPVDMTPTDNIAQHYYSNSNDSDDYKQPPQIPSALRAGSPAMSQPVGLPSQPRAGTAPPRPGLPASLQSAIQRREASQPLPNRGMNGSVQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRM1</accession>
    <name>A0A9W9CRM1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_000051</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-binding monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRM1"/>
    <dbReference type="OrthoDB" id="74360at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051209">
        <property type="entry name" value="FAD-bind_Monooxygenase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42877">
        <property type="entry name" value="L-ORNITHINE N(5)-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42877:SF6">
        <property type="entry name" value="MONOOXYGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G15050)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4377228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="626" mass="70486" checksum="AE09001FCB651A0C" modified="2023-11-08" version="1">MAPKSVIPAADKTTSDAQTSNSTRRVAQLTGHLASANETEQVNAIPSPPVDYPVSRLKLDFDRSIDQVQELKVAVIGAGLSGINAGILLPAKVPGIRLTIFEKNSDVSGTWLENIYPGVRCDIPAHVYQSTYSPNTQWSEIFAQGSEIRDYWQSQAKKHDVYKYVKLRHRVDEAEWDDAQSLWQVKVHDLVNDKTFTESFNFIITAIGRFNAWKLPDYPGLDEYKGFLRHTSNWDPSYDPKNKKIAVIGNGASGIQVVPNLQLVAKHVTHFVRNPTWIATSWAGDERTFEPQPYPEEQRKSFEDPETYLAFRKDLEDRYWRRFRSVVRGSTENVGMRDLFINVMKKRAAKKPELLDGLIPDFAPNCRRLTPGPGYLESLTEDNVDLVKTPIKRFTATGIETVDGKTYDFDAIFCATGANTDLVPPFPIKARGVDLNKAWKPEGKWGFPYTYMGMASPGFPNLFFLAGPHATGPSGTVPQAVETALTYFAKVFRKASSQGIKSLTPSKEAADDFIDYCDAFFPTTVLTDNCSSWNNGGIPGQRIHGLWPGSAAHITIVRREPRWEDWEYERENKINRFAYFGNGYTKAETEDDTDLTSYLKAPGKEVDLRDLHESWWDTPGARKKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRU1</accession>
    <name>A0A9W9CRU1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Queuine tRNA-ribosyltransferase accessory subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Queuine tRNA-ribosyltransferase domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_000144</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Non-catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer of a catalytic subunit and an accessory subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the queuine tRNA-ribosyltransferase family. QTRT2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRU1"/>
    <dbReference type="OrthoDB" id="27601at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008479">
        <property type="term" value="F:tRNA-guanosine(34) queuine transglycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03043">
        <property type="entry name" value="QTRT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028592">
        <property type="entry name" value="QTRTD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050852">
        <property type="entry name" value="Queuine_tRNA-ribosyltrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46064">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE ACCESSORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46064:SF1">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE ACCESSORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA-guanine(15) transglycosylase-like" evidence="7">
        <location>
            <begin position="31"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="479"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="503" mass="55867" checksum="BAEA14A781FD8DE7" modified="2023-11-08" version="1">MSVESSMQKLDQLPPEMLEFTLLKTTGALAPRLGRLSLSGRKSILTPGFIGNTSRGVIPHVSQDNFRKGVDVNGVYVALEDCKCLVEKHPQTTPPIFSHDVPESLRDFIALPQDTLLVLGARRNPPIYSPVPNTNTEIGVLTSVGFKSISSEYYAAAARKLQPDIVVGLADIPFGQESVGIKRKDKMSDRTENWLRDIIAKRDSIEEGEKSWGIFAPLLPIDRDLQSWYLEHLVDEMVDKINGVAIYDAYLLDDLPDQLHHLPRLSFHAPASPHELLRQISLGMDIFTVPFLADATDAGIVLDFKFPALAKDDDSSTRRSLGLDMWSDSHACSVTPLSEGCGCYACTKHHRAYLQHLLAAKEMLGWVLIQMHNHATLSAFFTGVRASIEAGTFDADVAAFEAYYEPTLPEKTGQGPRVRGYQFRSEEHAKKEKKNPKAFRKYGEDEIVKLKAAHGQQPNSRPEMRDVIDDEALLGLVGMDDSEAHADPDVQIENLRIEDDAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CS79</accession>
    <name>A0A9W9CS79_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PLC-like phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000523</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CS79"/>
    <dbReference type="OrthoDB" id="1046782at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF143">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C X DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="34458" checksum="493A8584999547D0" modified="2023-11-08" version="1">MDAWSWPTVSAEGKATSVYVEFGTKGNTRDDAGETYYNIAGTTSKFTVLGRKSSDYDLTISLDGMSTKQSPQGSKIDMGFRHDAAVNWIMSTDESGQWWSNSGSYITDWMQQSMGSLANRTLKQICMPGSHDAGMSKFTPGTVGANFANTQAQYLDFSQQLMAGSRFFDLRPVISNGQWVAGHYSALENDVEDIWVGGNGQSITDMIKQINDFTAQYKELIIINLSHALDTDNQYKNLSQDQWNRLFETLKGVNNRYLASNPGNHDFSNEVLGEFITDRASVFIVAQLPSDITLGDYANQGFFSTANFPCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSB4</accession>
    <name>A0A9W9CSB4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SNF1-interacting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SIP5</name>
        <name evidence="3" type="ORF">N0V83_000629</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIP5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSB4"/>
    <dbReference type="OrthoDB" id="21471at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24139">
        <property type="entry name" value="SIP5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039301">
        <property type="entry name" value="Sip5/DA2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31315">
        <property type="entry name" value="PROTEIN SIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31315:SF1">
        <property type="entry name" value="PROTEIN SIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="173"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="393"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="486"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="556"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="184"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="556"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="566"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="601"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="626"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="637"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="676"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="699"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="726"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="753"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="774"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="785"/>
            <end position="796"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="861" mass="93088" checksum="0C022EEF75BB3EEE" modified="2023-11-08" version="1">MGNTQGKESQPASRGHARRSSAQVPASPTTSGPVAPSQERTGTGVYSSRSGRGSRHDLSFLGIRAGSGENTERDPALEPRRETKAEREARKLEKDRVLRAQERERSIREEGVDGGFLVTLGVYTGPEDFSKPVVRQLQIERRLAPFWKGLDDHEDTWTEHQLVAVVNGLPLPAPDEIPTGEPPRTNNHLSPAWNPRSSEPNLSNLTVPIGSRSMSANSDRSGNLSPSHPTFSLPSPASPISSSTPFFRGRAKTLASLATGSRNASQTDMAPQELQLPKDPYVNGQRVEAFLYKNASECPICFMFYPPYLNKTRCCDQPICSECFVQIKRPDPHPPEHHGDAESPPAEPQEEIQLVSEPAACPFCVQPEFGVTYEPPPFRRGLLYSGQGRNPLGSATSAMSSTSSLNSPNVVSPGRRRATSLAANDKTVITTDMVRPDWAKKLADAKAHALRRAAAATALHNAAYMMGSIQQQESRFGLGRRRRMFANDSAGSSGQGTPRREGEASSGRGPEGSNDLFPGRISSRRGNRIDDLEELMMMEAIRLSLAAEEERKRKEEKEAAKEAKKEGKKKAKENKKVAKAQRNIGSGFHPVEIDGVDDADAGTSAAAGKGKAVDRSAGAVGFNPMTEPTSTINASTAKDDPQKHLEDSRAQIQRGTLEPPNLSPFDPFGEQPSYRSALRNMSNASSSASSFAESYQNSLRQDSQNNLAPSSAYGPSPNASGVSLSQGETPPQGTPETEPMFNFQSLAEAINPEDGKDENATQHIENIEERKSDSTNTAHATSDMATSSAQANTASSEPLGESVMTLKPSPPAPVLTVTNEDDDEITPAPRVEVVPDDHSFDQKQYGDVNMVDTLSHHQATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XWS0</accession>
    <name>A0A9W8XWS0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="19">AAA ATPase afg3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">AFG3</name>
        <name evidence="19" type="ORF">N0V83_010712</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XWS0"/>
    <dbReference type="OrthoDB" id="1413014at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005745">
        <property type="term" value="C:m-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000019">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000003">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1690.20:FF:000003">
        <property type="entry name" value="Mitochondrial inner membrane AAA protease Yta12, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011546">
        <property type="entry name" value="Pept_M41_FtsH_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF2">
        <property type="entry name" value="AFG3 LIKE MATRIX AAA PEPTIDASE SUBUNIT 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06480">
        <property type="entry name" value="FtsH_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="18">
        <location>
            <begin position="424"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="44"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="164"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="852"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="54"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="166"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ4361772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="883" mass="97511" checksum="58C493C5E1A3F049" modified="2023-11-08" version="1">MSTLLRRSGHLARLSRPCLSASSRAPLTLRRRVPIPQCIYAQQRARRFATGPPEEPKNSKKDEEIEKKQGEAAKTEPAEDSKKDAESQLSKLTPEEEKLLDQLVSFVSMGVPKSQKHAIKEAMDEIKKTGIPPELRLEMDRLARGEKLDLATAAKISRLTTNMARKAAEQQRFKDEKTDEPQQKSGAHDPGPQSRGKESKGGAPGAGGPNPMFGGSMDTNTILITAFLTYLTYKMLFPGENTKEITWQEFRTTFLDKGLVEKIVILNGNKAKVHLHREAVASMYPDSPSVNQNFFYYFTIGSVEAFERKMDDAQYELGIPSSERIPVAYSNEISWFGTFLSFGPTILLLGSLFYFTRRAGGGGGGSSGGIFGMGKSRAKKFNHETDVKVKFADVAGMDEAKQEIMEFVSFLKDPSRFQKLGAKIPRGAILSGSPGTGKTLLAKATAGESGVPFFSVSGSEFVEMFVGVGASRVRDLFANARKSTPCIIFIDEIDAIGRARSKQNFGGGNDEREATLNQILTEMDGFNTTEQVVVLAGTNRPDVLDKALMRPGRFDRHINIDKPTMDGRAQIFGVHLKKIITKEDMEFLKGRLAALTPGFSGADIANCVNEAALIAARHNAETVTMVHFEQAIERVIGGLEKKSLVLKPEEKKTVAYHEAGHAICGWYFKYADPLLKVSIIPRGQGALGYAQYLPNGDTYLMNVNQLMDRMAMTLGGRVSEELHFPTVTSGASDDFRKVTQMATAMVSKWGMSKKIGYIYYEDDSNGQQLQKPFSEDTAKNIDMEVKRIVDEAYKQCKDLLTEKKTEVGLVAEELLKKEMLGREDMIRLLGPRPFEDPADFHKYFSGEFGKAPGLIEPKGDDGVKGPLVPPPEPAAFRDLDARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYN5</accession>
    <name>A0A9W8XYN5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_010329</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYN5"/>
    <dbReference type="OrthoDB" id="10009520at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20353">
        <property type="entry name" value="Rcat_RBR_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051628">
        <property type="entry name" value="LUBAC_E3_Ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047546">
        <property type="entry name" value="Rcat_RBR_RNF216"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047544">
        <property type="entry name" value="RING-HC_RBR_RNF216"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058758">
        <property type="entry name" value="UBA_RNF216"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770:SF42">
        <property type="entry name" value="FINGER PROTEIN (ZIN), PUTATIVE (AFU_ORTHOLOGUE AFUA_4G03910)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770">
        <property type="entry name" value="UBIQUITIN CONJUGATING ENZYME 7 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26200">
        <property type="entry name" value="Rcat_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26191">
        <property type="entry name" value="RING-HC_RBR_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26112">
        <property type="entry name" value="UBA_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="435"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="272"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="10">
        <location>
            <begin position="414"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4363209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="629" mass="72014" checksum="4518E3BD1162A6B8" modified="2023-11-08" version="1">MATVFSSLIRPPKARRASSSNPEVAGPGPSLDSHGLDEPDLRDLNVSLQALTDMFPDIQPEVFREMLVSFSEESRLQVVTEALLKHGHKYVRGRYRMPAEQEEQRETAQKYKYRKEDAPKDTRGIPLALEDTFRSKRYKEAAKEALYLEFKGLSHSTIKAVLAEYNWSYTQARPTLLGLSTKSWRSSITNFLMRRKAPTADDHPLVIWVAADAKTNRPRMPLLMKSSSDELNKELYEALIVPEVEKQRAEQLQQDFELALQWNEEEAETEGEMYDCECCFIPNTLQQMSTCDVDGHYICFRCIRHAIKAALYDQGWARNIDTALCTLKCIAPMTDGVADCRGCIPLAFIKRALCEERDGEDNFNKLNERFASETLLKSQLPLARCPFCSYAELDDLALPDTNLFANLRLKPRPILVASIASIPILELLCYTATQAMVFFLVFSSTFFALLFRIPVIQPLHDALRRLHLKRRGLRFQCLSPTCGRASCLSCNLPWHDPHTCFSSQLTSLRLTLERATTDAVKRTCPQCNLGFVKSEGCNKLVCLCGYSMCYICRQGLAGEGYQHFCGHFRERPGQRCTECNKCDLYRVEDEERAVKRAKERAEAEWWERQGRGRRRGWKRRLGRRETVGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYP2</accession>
    <name>A0A9W8XYP2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribose 5-phosphate isomerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_010301</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LacAB/RpiB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYP2"/>
    <dbReference type="OrthoDB" id="2106730at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1400.10:FF:000004">
        <property type="entry name" value="Ribose 5-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1400.10">
        <property type="entry name" value="Sugar-phosphate isomerase, RpiB/LacA/LacB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011860">
        <property type="entry name" value="Rib-5-P_Isoase_Actino"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003500">
        <property type="entry name" value="RpiB_LacA_LacB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036569">
        <property type="entry name" value="RpiB_LacA_LacB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051812">
        <property type="entry name" value="SPI_LacAB/RpiB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004051">
        <property type="entry name" value="PRK05571.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02133">
        <property type="entry name" value="RPI_actino"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00689">
        <property type="entry name" value="rpiB_lacA_lacB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43732:SF1">
        <property type="entry name" value="RIBOSE 5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43732">
        <property type="entry name" value="RIBOSE 5-PHOSPHATE ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02502">
        <property type="entry name" value="LacAB_rpiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89623">
        <property type="entry name" value="Ribose/Galactose isomerase RpiB/AlsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4363181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="31719" checksum="0FFB7775C910726B" modified="2023-11-08" version="1">MSPPKRALFSLNLDSDADHVYQRVDDHSKEGTDKVKVIESKGPTHNHTDLSYVTKPFEKLVNRTVDGVIEGTGKKVHSAEGELTYSTERSEKKQGNSRLNVVSKKKRRHIEDKYDTSELDNAALPPLRIAIGCDDAGVSYKNAIIKDLSSDARVSTITDVGVPEHADKTAYPHIAVDAAKLVADGKADRAVLICGTGLGVAISANKVPGIRAVTAHDSFSVERAILSNDAQVLCMGERVVGIELARRLAKEWLGYRFDKGSASAKKVEAIMEHERKNYAGLVQDQQNGSRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYW6</accession>
    <name>A0A9W8XYW6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytosolic neutral trehalase</fullName>
            <ecNumber evidence="2">3.2.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Alpha,alpha-trehalase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_010605</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 37 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.28" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYW6"/>
    <dbReference type="OrthoDB" id="10036721at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004555">
        <property type="term" value="F:alpha,alpha-trehalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005991">
        <property type="term" value="P:trehalose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001661">
        <property type="entry name" value="Glyco_hydro_37"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34987:SF6">
        <property type="entry name" value="ALPHA-L-RHAMNOSIDASE SIX-HAIRPIN GLYCOSIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34987">
        <property type="entry name" value="C, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G02880)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01204">
        <property type="entry name" value="Trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040844636" description="Cytosolic neutral trehalase" evidence="5">
        <location>
            <begin position="19"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4362511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="582" mass="63025" checksum="4A945828E544CFAC" modified="2023-11-08" version="1">MKLLSILTGLAILDSGLAIPYSEYILAPKSRTLHPVSVHNTWGPVTNPSALLTTSSTSSPDSNTTTTFHSTSSLTYDFGLNIAGIVSLNTVSTSASNQTIGVTFSESSLWVSNKSSDATADAGKDEILWFEIEGPGVVTARKEKGRGAFRYLTVVYEGTGSIEVGGLEVHYTAMPHWEDDKLGEYTGWFHSDDDLLNRIWYAGAYTNQLCTIDPHYGDALVHIFQINASVSDATNVTWWSNYTITNGSSALVDGAKRDRIVWAGGKFQTLSKYMSIAIPGIVVSTNDVVSVENSLTSLFDIQNPSTGQLPYAGRPFPSGNISYLRTLWPKYKLALHFSLSYIDSTGLMNVTSPADWLRSGMGGHNIEANAILYHTLNQATLLASVLNDTSPSISYWSLTASRIKQAANALLWNNEAGMYIDNETTTLMPQDGNAWAVVSNLTLNASQISSISEKLQGRWTAFGAPAPEAPVPSSNISTISPFITSFELQTHFLASSAHTALALIRRQWGFMLTSPLMTNSTFIEGYSSDGSLHYAPYANDARISHAHGWSTGPTSLMTFYVAGVQVTGKGGGEWRIKPMLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYW9</accession>
    <name>A0A9W8XYW9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_010335</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYW9"/>
    <dbReference type="OrthoDB" id="9996127at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4363215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="215" mass="23926" checksum="FBEB85167C0BBDFC" modified="2023-11-08" version="1">MLNPVIIMYIIQEGVDAVDSKYTQAFHDLGPLVVSPQSGTYKDLAAWTDIALDSPPCQDFGFNNPRFPIYIKSYNVTAQKIAYDLYASAVSGANNTYTNSIFMFEDYATGGVRALDTSATAFGFRQDLILAAPLIIYEPTDKARDTAVGQLGNKLREIIREGTGSTEVHSYVNYAYGNEGPKGWYGYETWRQDRLKALKKKYDAKGKFSFYGPIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ86</accession>
    <name>A0A9W8XZ86_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartate aminotransferase</fullName>
            <ecNumber evidence="7">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">aat2</name>
        <name evidence="9" type="ORF">N0V83_010117</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ86"/>
    <dbReference type="OrthoDB" id="6752799at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006532">
        <property type="term" value="P:aspartate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000064">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000001">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF55">
        <property type="entry name" value="GLUTAMATE OXALOACETATE TRANSAMINASE 1, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="8">
        <location>
            <begin position="33"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4363000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="415" mass="46243" checksum="98D605EC357955A1" modified="2023-11-08" version="1">MAATSFNADVVPQAPEDPLFGLMAAFRRDQDPKKVDLGIGAYRDDNAKPWILPVVKQADERLRDDPNLNHEYLPIAGLAEFTSASQKLVLGGDSPAIKEKRVTSLQTISGTGAVHLGALFLAKFYKPNTERLTYFSDPTWVNHFQIFSNVGLSYKTYPYFSKKTKGLDFEGMVGALQDAPEGSIIVLHACAHNPTGVDATQDQWKKIAEVIKSKKHFPFFDTAYQGFASGDLARDGWAIRYFVEQGFELCIAQSYAKNFGLYGERAGCFHFVTSPSSDAESTISRIASQLAILQRSEISNPPAYGARIASTVLNDPQLFKEWEANLRTMSGRIKEMRTALRNKLEEMGTPGTWNHITDQIGMFSFTGLTEQQVLKIREDSHVYMTKNGRISMAGLNTHNVEYFAKAVDKVVRETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZQ9</accession>
    <name>A0A9W8XZQ9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">RNA helicase</fullName>
            <ecNumber evidence="15">3.6.4.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">DBP8</name>
        <name evidence="15" type="ORF">N0V83_010958</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="15"/>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZQ9"/>
    <dbReference type="OrthoDB" id="10261904at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17955">
        <property type="entry name" value="DEADc_DDX49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF24">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 10 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="14">
        <location>
            <begin position="85"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="116"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="326"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="9">
        <location>
            <begin position="85"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4362017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="514" mass="56602" checksum="90A88A1914E4097C" modified="2023-11-08" version="1">MTSSYSDSGEFDEDIMEAQSGARKRRRLSPSSDESANSDTETTPKIPTSTISRIKSKQQQPPQPPTDRQPGPAVTAAVARTGEHVSFASINVAPWLVASLSSMEIKKPTGIQKACIPEILKGRDCIGGSRTGTGKTVAFSVPILQQWAEDPCGIFGLIITPTRELAIQIYEQVKAISAPQSMKPILVTGGADQREQAIALASRPHVVIATPGRLAEHIKTSGEDTICGLRRVRFVVFDEADRLLAPGKGSMLPDLETCLSVLPPKEKRQTLLFTATVTPEVLALKEQPRPGRQPIFICEVDTETLAIPPKLQQKYLQTPVTHKECYLHVLLSTPENLKKSVIIFCNRTKTATLLEYMLRLLQHRVTALHSGLKQSDRVGNLARFRAQAARILVATDVAARGLDIPEVALVINYDVPRDPDDYIHRVGRTARAGRVGTSVTLIGQRDVELILAIENRVGKKMEEFEEEGVSIEGRVVRDALKPVTEKKREAMLSIEEGRDVLGKRKTGMQRRRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZU2</accession>
    <name>A0A9W8XZU2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sfi1 spindle body domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_011004</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZU2"/>
    <dbReference type="OrthoDB" id="5215300at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013665">
        <property type="entry name" value="Sfi1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08457">
        <property type="entry name" value="Sfi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sfi1 spindle body" evidence="2">
        <location>
            <begin position="353"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="926"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="992"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1184"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="927"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="941"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1004"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1075"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1124"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1201"/>
            <end position="1212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1221" mass="140425" checksum="911AA1752209269F" modified="2023-11-08" version="1">MTAHGYDTPDLTKDDLTDEAIETLYDIVRRAQLSSSGQPSHPSDPNPPSSRALFAAYEDVLEELGLSSSDDVVLHRFLFRMQENRRKDESLMQRFKRVLGEFNITFEIDEDGEVTTNLDATRNRTRNSVGRHSRRGSFDSFFDGTADKIAGTDYGDLPVRARRASQGALNGHGTRESQRRTRSETEAQLPFRNKVNGNAHQRSASGHYQSNRKRSASVSSRGSLQIRRDVQTWNSGARDNDDGSEYTERTTSLDLSHVQIPGLNAPIPGDDQESSHQQQYISEPWHPSDTRLMDEAEMFEEQRLHRVTRDCIQTWRNRTQERLSIRDDMENLAAARDRRILLKLSFEQLRDTLRMRRSAQETDRFFTRLESRADKARNLFLLTKAFTHWAKCTEDEVQRTSVARRHILRTRFFNGWRDITAVNELKIQHFVLSKFLRQWRAKAATIRDNSEFAVQLYEENLVHRVYKQWFFKFCAIAAPAWRNDRIRRVTLQKWSEIAKVLRERENWVTDRRDRTVLRTTFQAWQQNTTKVRALEPQADGLRQKRLLSLALRTIQKQAQLAPLLRQFQARVDGRLIRTTFQTWHRTSQLSRQARNVDRLRILRNAYTAWNDRLRIKALEDRINDRVIVECLYKWTLASRVSLFQRVHDRQLKESAFLTWVTKTNQQANTIDAAKRRFAQFKRTQLLRTSLRKMEAITAEKKAEEFAVVAEYQQKLKQRIFEVLKEKLEHFQQLNQWSADASFYVLGKRTLRTWSEATQHARRNRRREAYAQVRRMTKMNLVRRVFETWRNKANHVAVLNQQANDMLENRVLQSTGSFLHQWNDRTITLQQQDTQAINLHNFKLETRCLSTWTHRFETLRTLESQAVALRQESTEIAAASALKKLGWRLWNIQRQEQNAQALYERNFEKHVRAMIRFWLEQAAERLATRPVSPTPTSRSRGSRRDRDDGHAQGDGDGGLDEADDETHRLENWTAFDEDALGLNNDLDLSLSITPDHPRTYVNPYAHPPPPPPSSSRPPPQQPPLSSSRRVPPPRPSTYPQPQSALRPRPPHPPTIFEDDQSDLDFGAQSTFWSGTPMPPPPPPLPLPLPTTATAAVSSSKPGYLKTPSKRSVVRAKRPELLPASPEKRDRDRDFSPQRRRLGAMSAPPAQVTRAVGGGGLGAGGVVGASGVSGITSFERRMQESGFSRSVVGGGSGGALARGRGRGGKGKGRVGFRDVSEMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0M1</accession>
    <name>A0A9W8Y0M1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0M1"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="178" mass="21238" checksum="30B0EDF5EC12AE42" modified="2023-11-08" version="1">MYSEEVREKRHQERNVQTRLLRHRNPAETEELRTLNQAAFGANRKIKVAEDARSKQGRRPNTEERETEDGLRRVVEQYGRRRDEINAATTERLNKEAEQKERERQAKNKEKEEKWKREEADKKRKEAEKQAAKAKKEAEKKRKRDPKSKSRSPGEQDRWGNYVNKVEKGESSRGGQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0X5</accession>
    <name>A0A9W8Y0X5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">inorganic diphosphatase</fullName>
            <ecNumber evidence="3">3.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_009814</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0X5"/>
    <dbReference type="OrthoDB" id="1608002at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="175"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="175"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4363519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="200" mass="22130" checksum="4A6172280F0A60A9" modified="2023-11-08" version="1">MIGAKVETKRDAPLDPIFHDEKDGTPRFKESVRSHKAMPFLHGSIPQTCESPNVNCSFTGYPGDKDLMYLFDIGQDPGYTSQVKQVRVLGGLSFVDVDETDCKSIGPDITDPLAPLVDSVDDVERPTVKKIQESNDFWKDLGNGKADPGKIEFAQTSNGELESYTSCKDATKDFKLPKKSKTGKEAEKPEKWQQLNSTIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1F3</accession>
    <name>A0A9W8Y1F3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_009749</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1F3"/>
    <dbReference type="OrthoDB" id="3257095at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF4">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="329"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="413"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="460"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="490"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4363456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="58135" checksum="B1741828EF8F7F6C" modified="2023-11-08" version="1">MSKDTQIETSVPKSPSYDDENHGEEYHDSVRGYTRADRADMLRMGKVQELRRNYRPLSALAFTVILQGTWEVLLTATSQGLIDGGPAGLIWSFVWTWFGFSTVMLSLAEMASMAPTAGGQYHWVSEFAPPTIQKPASYFIGWMSTLSWQAGTASGPFLVGTLIQSSAVVMYPDYSPTNWQGTLMVIAVTILVWVSNLWGARMMPMFQNIMLVVHVFGFLTIIIIFWVLSPRASAEVTFTQFTNSGGWSTTGLALMVGQLSAIYACICSDSAAHMAEEIKDAGKTVPRAMIGAYLMNGALGIIFLISYMFMITDVEAALDDATGYPHIWVFSQAVSAGGVVALNAIPTVLIFAGTLTFNLSTSRQTWAFARDRGLPFSNWIGHVDQKLQVPANAVTVTCLITIVLSFINIGSDVAFNAIISLNVVALMITYVVSIGAVLYRRVRHPELLPACRWSLGQWGVPINIGGVLYSFHAFFWCFWPEGTPVAVESFNWAVVMFVGVFVLSLVDYVIRGRKQYKGPVVLVEGFKGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1M6</accession>
    <name>A0A9W8Y1M6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009299</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ustYa family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1M6"/>
    <dbReference type="OrthoDB" id="3687641at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043386">
        <property type="term" value="P:mycotoxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021765">
        <property type="entry name" value="UstYa-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11807">
        <property type="entry name" value="UstYa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00035112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="61" mass="7198" checksum="036C620BD760D873" modified="2023-11-08" version="1">MQILRMNLMCLSDINVFTFHKLPGERAKEGYWPDYESHHVCRNFDAIKQWANDNAMPEADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1U4</accession>
    <name>A0A9W8Y1U4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protein SLG1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">wsc1</name>
        <name evidence="11" type="ORF">N0V83_008200</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1U4"/>
    <dbReference type="OrthoDB" id="2019572at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051836">
        <property type="entry name" value="Kremen_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269">
        <property type="entry name" value="KREMEN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269:SF23">
        <property type="entry name" value="PLASMA MEMBRANE SENSOR TRANSDUCER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040728214" evidence="9">
        <location>
            <begin position="22"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="189"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="10">
        <location>
            <begin position="44"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="140"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4365580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="283" mass="30308" checksum="DF3EBBB351FDBFB7" modified="2023-11-08" version="1">MVLIRLTSAIAAASMLLMAAADDTPTATVSNPIETPTVTLPASQISEIGCFETGTPLENHGDYLYQSPGNCQLICLEQKKNVMGLSDGENCWCGDLIPAKEWQTDNSTCDTTCSGDKTVRCGGPSVLWIALTGNTRNRVSYYEPESSSSSSSSIQPSSTAAPTSVAKATASSTPSAEPKEKSDSKPNTVAIAVGVVVGIIGLAAILFGVWFLLRRRRQRQAEEDYRRNAANVNAFVNGGKLHTSNSSMNDSRLDPSFMDRRQSNGSIADNEDYSRRILKVTNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2U8</accession>
    <name>A0A9W8Y2U8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008348</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2U8"/>
    <dbReference type="OrthoDB" id="10042665at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19481">
        <property type="entry name" value="RecA-like_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054289">
        <property type="entry name" value="DUF7025"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46411:SF3">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46411">
        <property type="entry name" value="FAMILY ATPASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22942">
        <property type="entry name" value="DUF7025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="445"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="651" mass="73930" checksum="AE10C4F21D522D09" modified="2023-11-08" version="1">MAVKETSNGVKDTNGTKGLAPEGSLASFKSLYQGPQDSNKKWTWLDKEPADVAEAAENEETAQHALITRLQKANDSRKKFETHSIIIQSPWLKEALAEILQDYPGIHCGLKRLEFEAPFSPFVHRWSEFLEFRARKDLDSVTSEHIELLHDVLRAELKDVIKTLEDYVDHGIVTYDHIWTIYQPGSIIYSDSHNGAHGAYRLRSGAYVKTQCGKAYQLSVEAIDWNGNHFGRSVESIRLWEFLGTTRILGLSAFPLSFHPHQDKVRELLINRGKKFEELAGCHYKSYDGFAITWDKEGNEVPYMCNGRIIVDSDSFRRFSPRFVRGIEPLAAKEGVQEPSCAPEKLNIEDPSNIKYVFGQQNKADRIRLTEEHHLICLPRVRGYSLKKKQWLLFYLDLVHDIDFNENAFGSLVLPEDQKELILSFAESQAMNHNSFDDVISGKGKGHITLLSGPPGVGKTLTAESVAEHMRAPLFMMSAGDLGINPDQVESKLTNILEMIAKWNAVLLLDECDVFLEARSTHDLERNKLVSIFLRVLEYYEGLLFLTTNRVDNIDAAFQSRIHISMAYPDLTSKSRRHIWENFLTGLGVLQEWKAEDLEDLAKVELNGRQIKNVLKSAALLSARKKDKLGRNYVDMVLGIERRRPGVAEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y368</accession>
    <name>A0A9W8Y368_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_007903</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y368"/>
    <dbReference type="OrthoDB" id="3941101at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4366267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="257" mass="28840" checksum="176B21477AE8DA6E" modified="2023-11-08" version="1">MTALQHINAFDLLDDVAPDEELPGYEAATAPAYSSGLYHEPLMTYSLRQYDRRIQMLVAYGSATTSSYRITTNNFRMFSKKPEMEVLYTSPENRQRNIASIAFNNNGAIPWRPRAHFDHTDSTGSTSTYNMESANFTDWTVGIRGVTYVWRVDMSPISIVLSELNSHIVIARFTYSACGTSAARGAEVGELTIFRDSLTSETDGIGKMVCSLMIALTQLKKMGRYYTNTVNELERSRSLRGEQLELRRTSTAAFSTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y387</accession>
    <name>A0A9W8Y387_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4364960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y387"/>
    <dbReference type="OrthoDB" id="10262413at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051783">
        <property type="entry name" value="NAD(P)-dependent_oxidoreduct"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48079:SF6">
        <property type="entry name" value="NAD(P)-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48079">
        <property type="entry name" value="PROTEIN YEEZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="354" mass="38446" checksum="0A25E333CED767D1" modified="2023-11-08" version="1">MGQNVLITGAAGYIGGSVLAEFVSRTSGSIPTAKINAAVRSEDQVQSLSGLGVNVIQLDLSEEADVLETILRNDIDIVLLASDAKALTGSQSSVTTLFSEEGGWIYGEVRDTDSIFEKEKELGESHPVRQTNIVVVEEASRQGVASYNLAIPTVSDGRGSGECRKLSVSIPQYVRTSIKHKTVYRFDKDGVSSSATLVGGKSQCTNTNQSPPAAHISDLTALYALLVEKIIQQEPFPSGKNGYYFAMAHRAPWWDVMDGIAKSLHARGLVDEDRVQVWQSDKMAAEYLGFPELYVRAMGTSTGELVPVNANRLGWEPKWDKERFLESIDDEVQAVLELDTVKATVFDALKTPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3F7</accession>
    <name>A0A9W8Y3F7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Adenylyltransferase and sulfurtransferase uba4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Common component for nitrate reductase and xanthine dehydrogenase protein F</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Ubiquitin-like protein activator 4</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="14">Molybdopterin-synthase adenylyltransferase</fullName>
                <ecNumber evidence="14">2.7.7.80</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="14">Adenylyltransferase uba4</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="14">Sulfur carrier protein MOCS2A adenylyltransferase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="14">Molybdopterin-synthase sulfurtransferase</fullName>
                <ecNumber evidence="14">2.8.1.11</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="14">Sulfurtransferase uba4</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="14">Sulfur carrier protein MOCS2A sulfurtransferase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="14" type="primary">uba4</name>
        <name evidence="14" type="synonym">cnxF</name>
        <name evidence="17" type="ORF">N0V83_007822</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers urm1 and MOCS2A. Its N-terminus first activates urm1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to urm1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards urm1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; nfs1 probably acting as a sulfur donor for thiocarboxylation reactions.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers urm1 and mocs2a. Its N-terminus first activates urm1 and mocs2a as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to urm1 and mocs2a to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as a nucleophile towards urm1 and mocs2a. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; nfs1 probably acting as a sulfur donor for thiocarboxylation reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H(+) = [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:43616"/>
            <dbReference type="Rhea" id="RHEA-COMP:12159"/>
            <dbReference type="Rhea" id="RHEA-COMP:12202"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:90618"/>
            <dbReference type="ChEBI" id="CHEBI:90778"/>
            <dbReference type="EC" id="2.7.7.80"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 = [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48612"/>
            <dbReference type="Rhea" id="RHEA-COMP:12157"/>
            <dbReference type="Rhea" id="RHEA-COMP:12158"/>
            <dbReference type="Rhea" id="RHEA-COMP:12159"/>
            <dbReference type="Rhea" id="RHEA-COMP:19907"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:90618"/>
            <dbReference type="ChEBI" id="CHEBI:232372"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="14">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="14">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.80" evidence="14"/>
    <dbReference type="EC" id="2.8.1.11" evidence="14"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3F7"/>
    <dbReference type="OrthoDB" id="10261062at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061605">
        <property type="term" value="F:molybdopterin-synthase adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061604">
        <property type="term" value="F:molybdopterin-synthase sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2 14" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="12 14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
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        <location>
            <begin position="409"/>
            <end position="527"/>
        </location>
    </feature>
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        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
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    <feature type="active site" description="Glycyl thioester intermediate; for adenylyltransferase activity" evidence="14">
        <location>
            <position position="283"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <position position="128"/>
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        </ligand>
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        <ligand>
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        </ligand>
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        <ligand>
            <name>ATP</name>
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        </ligand>
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        <location>
            <begin position="217"/>
            <end position="218"/>
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        </source>
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            <dbReference type="EMBL" id="KAJ4366187.1"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="529" mass="57322" checksum="08D1D47906BA03FE" modified="2023-11-08" version="1">MVAVIDSLRQQIASCEATLRDLRQQLAEAEHVHRQQENALRKTKSPSTDPLDHDMNFGIPDDFRSEVFAILQHGAATQEQDDVDKQRKWPLEKNEYTRYGRQLIMPEIGLQGQLSLKQARVLVVGVGGLGCPAAAYLVGAGVGTVGLVDGDVVEISNLHRQILHSTARVGMTKVESAMVALSSLNPNVKLIPHISRLIPETSVSIFKDYDIVLDCTDTPASRYLISDTCVLLGKTLVSASALRIDGQLMVLNNPPLPPGDPKGGPCYRCVFPKPPPPVSVVSCGDGGILGPVVGVMGVLQALEAIKVLTQKPAGTDAAQVDPPSLLIFSAYSSPMFRSIRLRSRKLKCAACSVQATVSPEVLESGSLDYVQFCGSISPINALTPRERISAENYAQLRSGVNPFTGTVSSKDNHILVDVREKVQFELCSIDGSVNVPFSAVSATSSLSHDVNTPSPRSNAMEVDENDWVTHLRQTEKPIFVVCRLGNDSQVTVKKMKELGLDYGGKRYIGDIRGGLQAWRKGVDADFPNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y465</accession>
    <name>A0A9W8Y465_9PLEO</name>
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        <recommendedName>
            <fullName evidence="1">Tyrosine specific protein phosphatases domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">N0V83_008790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
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            <taxon>Eukaryota</taxon>
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        </lineage>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAJ4365172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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            <begin position="15"/>
            <end position="97"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ4365172.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="166" mass="18439" checksum="74EB53C837A14BAE" modified="2023-11-08" version="1">MSDFLEEGGVKAYTAMYLEILDVYAPSFKIALEWLRDEKTGILFHCTAGKDRTGVLAALLLSLADASRDLIAYDYALTRVGVEPSREVLVQVLRLWNAEWTEDTPGMHEFTQVKGDFILGLLDAVDLKYGGMERYAIAVLGFEVEELDVVKSVLRGELSKSQGGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y478</accession>
    <name>A0A9W8Y478_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_007345</name>
    </gene>
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        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="4135672at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="DUF6540"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20174">
        <property type="entry name" value="DUF6540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4366820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="143" mass="15546" checksum="4ED6C881BE0A8B74" modified="2023-11-08" version="1">MRGTGTVVDCKKSIAQSSDDLGDVLLRLRGGAPRNHVGIFVEVNEDLSGSLFQVTGSIQNGMTFGHKKAYRPEDSASYVSKTYVGTIAKDDYARIQAIVEAIPPPPKQFNGPRRIDPSKPLITCQEWTKEAVQALKDGGVLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4M9</accession>
    <name>A0A9W8Y4M9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4M9"/>
    <dbReference type="OrthoDB" id="5419922at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="623"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="715" mass="78328" checksum="A98E4D9598DA3796" modified="2023-11-08" version="1">MPPTLHPHLKRWLDFDEFYTDPEDVIEEIPTHYHDHEERTAKRRRVEIIAAQYLRGKVPVILSAGLRGPFNKGWKNPWAERSLEIPKLSSGDKGMVARRSRPSQRSSAGGMSTAKARKDRKKVADAAQEGARIASPEASRAAKEDLGLETHQRDDSLDAVEVPPATAPSKVDDNASGATEYFSANTEKIIRKRSPLTNPFWLRRPVTEETRNMNQPSNSCTDVSPTRSRSRKGNSQLDVTEGLQIALPKASLHQEGSPARVAMPDECRSSASAAMIISSPMQRSNTWSNTKISLPSSIGGQGHPEIPIAESAVQQEISLHVKLAHCTTPNGEATPEDVRGLKDAEHRIAVLGSQRRPSRKDIQQSADRLVSLMPSSTRRQAYSKNKAVQNRAVRTAPQHNLVASPAPASSTGFIYRKLGVSKHNGRSAQTSKAKVINFEPSPAATNGTIVSDERTLDKTGNTPNPESCTIFTHQLDTAREETAYKLYSGEVAKAHDKDLHEEPDQEPSRAHLVSTNSTQAAMLLAQKVFQEGAFSTMSLDTPRQLQHDTPRPLLPDPSPAITPLPGFDGQLDKVLPDASVLRGPPISTQDLFGATSPFAFSTVKKKPEISQRSSLRFALLQTDDEQSKTSAAHAKSPTPSAERVPLKDKNTTTAFWSFVTEKASQASQESSVGRTRRSIGDVGLPQLDFHTSLDDFGPNGDLHFTDRFLRNLDGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4V1</accession>
    <name>A0A9W8Y4V1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Programmed cell death protein 2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008306</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4V1"/>
    <dbReference type="OrthoDB" id="443682at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007320">
        <property type="entry name" value="PDCD2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47524">
        <property type="entry name" value="20S RRNA ACCUMULATION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47524:SF1">
        <property type="entry name" value="20S RRNA ACCUMULATION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04194">
        <property type="entry name" value="PDCD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Programmed cell death protein 2 C-terminal" evidence="2">
        <location>
            <begin position="274"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="414" mass="44951" checksum="926375C8A5AB177F" modified="2023-11-08" version="1">MPPYDSESSDEGEDYTETNVLLGYATTEATGDAVSHLGGAPNWIDNKTAPSGTLAKCKVCNGLLSLLLELNGDLPEQFPGHERRLYIWSCRRKTCRRKQGSVRGIRGVRIAKGASNKVAAKVEKKEEKKQEEKPQPKIGESLFGVKDSQSTTAPANPFSNPFSSKPNGQTPSAPAESDTKQDEASTTLPESFASKVRLSSPLPSQQPARPREPWPAESSFPTPYPLYHLDAEYETLDAPSTPQIPANARMDIDAEGSGTGGGKEDKDVFESSLDKTFQKFADRVGENAEQVLRYEFKGKPLLYSDHDAIGKLLAHHSENGPSSNAKITTVSSRGGASIPPCQICGAERVFEVQLTPHAITELEADEMSLDGMEWGTIIMAVCSKDCKPSDVSEGEVGYAEEWVGVQWEEVASKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y559</accession>
    <name>A0A9W8Y559_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y559"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="243" mass="25407" checksum="756FA71186D64B2C" modified="2023-11-08" version="1">MSSRNTHRSSSSFSQQHQAVPSSRMTSQRVPAGLTNIGGMGTGTAAPSSSRIGSHRSNAPTGSRLPTARVPTAAAQPPIGSRRQSQQQMLDVPLVGNTHGHGGGSRSGMNMNGMYPSAAPAPSRSASVHHSNSMMMNAPGPSRHQSMSMAPNGALMRPSMAARLRNEAAMGGGMAGAMVSYAPQNDMARQQSVSSSRGMSSRGMETRDGRGGMMAPSRSDGRPPRGARSATYEYSYMKVTYHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5I3</accession>
    <name>A0A9W8Y5I3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007607</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5I3"/>
    <dbReference type="OrthoDB" id="5336600at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43669">
        <property type="entry name" value="5-KETO-D-GLUCONATE 5-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43669:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G03445)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="273" mass="29579" checksum="ECFE271E38921A94" modified="2023-11-08" version="1">MTRTLIVFGSGPGIGNNVAAAFASKGIDHIILLARNTQRLENEDAPFVSKGHNSVKVDTLRLDLANLDSIPSVITKIDHLTENEDVEVVFFNAARIKPSEPLGVSVQEIDEDFKVWSHYYYTTTTLLHSAVPLPNPTQPNHPKTQIANPLSLTQTTNLALYIIAQHYLPRLQTLASSSSSPSPLKPALLVTNSALPWDPIPQLLSLSAVKAAQANMVQSFDRAFAGSGVHIGLIHVCGQVAPGNKVLNPGTIAERTVAFWEVGRGVEVRIEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5L0</accession>
    <name>A0A9W8Y5L0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_006586</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5L0"/>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003823">
        <property type="entry name" value="SNF2 family helicase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF17">
        <property type="entry name" value="HELICASE-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040816162" evidence="6">
        <location>
            <begin position="19"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="503"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="1065"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="16"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="265"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="339"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="888"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1020"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1240"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="309"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="353"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="364"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="888"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1032"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1242"/>
            <end position="1252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4368230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1261" mass="140705" checksum="D8E6002B87260474" modified="2023-11-08" version="1">MVVFEILVLLAPFNMTEPIDMGGTQPLHPEVEPSSEETMTAGGDDDSQLRDFQDDGEEHEDNEGFIHAPGFSDPTEESQDNMTQDQEDKVYPSIEAPLLESETTIPDDADATSLFVPENGSPTPPVPRREGPAANMPPPPRPSAASRQPTAGISTLAKIRNMQKKYQEKKNASASRNPTHINTHADNEAYLEAVMSGITPTSSTPVPAVDEDDMADRQAIADFARNKRHYDELKLKNGGKLSFRHDIEWMKIKNAEDARKKKRQRDLAKARDSDDEDLNLFPTINPAVNDVQDNDSDDGTFNFDEFIPRKRPRQLPRKGPKQISMMDAELRSMQVALEAEGDMPRKKKKVDESQSSAASSARSKGSKAKSTKSSKAKAPPKKAGNGPRNTAKKKREVDHAVKQATSLFNSNVFQQQAGAGAIEQPTFTSRVKADALKELIASVPIGDQTKARGEMNTLLAATKEFDGKGSVKSDGAGHWLVKGMKTSLKAYQVLGTAFMRKRENATEQPRGGLMADQMGLGKTLMMLANIVNGQPPRGVHPRTTLLVASPSLLTQWKNEIEQHTDCKFKIMRYGAGTRVDSNNAFNIISAHDIVLTTYNEVMKSYPKNEPPIECQTAEQKIAWWKNTYDKQRGILHRGMFLRVVLDEAQMIKNHMGRTSIACRALIAHHKWALSGTPVLNSLTELYPYFKFLGVPHTGSFKIFKHNYCDNGGTESSERLLVRLSQFMIRRTHADEMFGAPILKLPQADQTTYWCEFNSIERQIYEIVRIRFSMRISMWAKKGTLEQGSYSNALVMLLRLRQLTAHVLMLQFVMRDLLEREDIERIKQVVEGQASNTQNGRTIVAIRKQLDKLAADEKKKSAAEAAAKKAAKEAGGNVEDANMPMDLTIDEVGDNADNEDNDKDEPEDATEEHVQGSRSRVGSGGLFGKEYNFKPFLNSLRTGESWEKAKKKAKCGTCKEQPKNPYMLSCSHLICENPCLVDADVEAAENGKQFADCKACGVCPAYCHPCEPEAEDFLEPVAQGTRSRTKSKKEKERKQRGNEDIAEDWLASVGDDVLPSSKTIAVKAQIMNWTRENPGVKVIIYTQFLAMIRILARVCAAENWQAEQYHGNMSLLARDKAISNFAENPNVKVMLASLRCGGLGLNLTMASKVIMIDPWWNSASEQQAFCRVFRIGQKEETFMSRLCVKDTVDERLVEMQERKDAEIEAVMNDDGSKVKKMNINDLMRLFGSDGTPYIMVDNPDPRGGFRADRDDEGYVDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5R5</accession>
    <name>A0A9W8Y5R5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphatidylinositol-3,5-bisphosphate 5-phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">FIG4</name>
        <name evidence="6" type="ORF">N0V83_006676</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5R5"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043813">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043573">
        <property type="entry name" value="Fig4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738:SF5">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="5">
        <location>
            <begin position="263"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="770"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="859"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="770"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="794"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="878"/>
            <end position="888"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4368320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1118" mass="127589" checksum="C6AB67281A45C6FB" modified="2023-11-08" version="1">MEATKSREEPQDRASSIASRATTSQDSSQERLDDIEKASTVVGSPLISPQDEVPNGLRAEVDLEKLPGHGKPRRESESTKFGGEEEDPNVGVSFQRSSTPSTIHKVKGLTYQHVGESGIKRMHKFSLYETSTRFFLIGADIMEKQYRVLKIDRTSAPGHLSIFDDDIVYNRREMYQLLSTIDDGNKATGGLKEKCSAWGLLGFIRFTEAYYMLLVTKRAQVAMLGGHYIYQVDGTEMIPLTTGSTSRFQKDRNPEEARFLAILNNMDLTRSFYFSYSYNVTRSLQQNLIKEREALNDGVVNAERDFQDMFVWNNHLLDPARDTFKNVYDWCHPIIHGYIDQQSLDVFGRRIYLTIIARRSRFFAGARFLKRGTNDSGYVANDVETEQIVSDALTTSFHAPGPRLYANPTYTSYVQHRGSIPLYWTQDNTGVTPKPDIDLSLVDPFYSAAALHFDNLFERYGAPVYVLNLIKARERTPRESKLLYEYKNAIEYLNQSLPNDKKILYEAFDMSRAAKTRGQDVISQLETLAEKVLRKTGFFQNGDPELDTPHVQNGVARTNCIDCLDRTNAAQFVIGKRALGRQLQALGVIAGSTVEYDSDCVEMFTHMFHGHGDAIAIQYGGSHLVNTMATYRKINQWQSSSRDMVESFKRYYHNSFLDSQRQEAYNLFLGHYIYEKGQPLLWELTTDYYLHHQDPRTWLGKVRRSYNDWYTPANLQPRTLPPVITPWEYGDQVPEAVISARDDYWLEYYRPAAISSFLKIFAFRLASPRAGRDNRDGTHPSRKENLSPFVPRKKRDDHGNDRDSKKGPRKGVTIIDPSSDTESRHNGITGRRRRDIHLSVPHADSPAKQSILRDPHFETQMPSSAPSATGAFRSMNNSTSTSTGLSSTLKPGFKPADKTLIYQWTLAQFYDNSMNPSVSANEEDEYSRYTEHPLSLPLVVSSEMPSADDPSAMEYYEYLCMTEGGIPAPKESDDDYIRINTTDFPRLDTDVTSIRSFTSHNTPRPMTAQSVSTPFSHAPSYSYPQQYPLHPHVHQSYPIDTTTTTTSQTYTAPGKFHTADEDIEEFEEFLNIRENPLDVEDDDGGKKRYKAYRQWLKGKSFFKQSKVDPEWQNQLPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y647</accession>
    <name>A0A9W8Y647_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_006407</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y647"/>
    <dbReference type="OrthoDB" id="432970at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="6">
        <location>
            <begin position="208"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="283"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4368052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="314" mass="35127" checksum="90E00F90237C9F50" modified="2023-11-08" version="1">MEPTLIPLDDFKLPDYPDASLNLNASPLTILDIKDFTNRHDTSKLTPAIGIATLLYNWCPESLKAFLDLDAWFSLTWNLSTNDNSPSETRLEIGRVGNQITFGSLNNGGESWTIMLTFNIATEGPERGSWVPNVKESMLGDEDVADAQEIERLARDWVREAILEKRWETGKGIRHRFFVEYAPMDDVWSDGIRMNPHWLYSALDLSACTTCCQKPGAGANATLLQRCGRCGTATYCSSACQKRDWAVHKDICTMGMEDRGQALAITRKGGLMMWDVEKTFAEVGSGEESRNPNFKEAQMRRVRRRDGDGDSVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y688</accession>
    <name>A0A9W8Y688_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
            <ecNumber evidence="4 12">5.3.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">5-proFAR isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 12">Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">HIS6</name>
        <name evidence="13" type="ORF">N0V83_007352</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:15469"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="5.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.16" evidence="4 12"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y688"/>
    <dbReference type="OrthoDB" id="446074at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04723">
        <property type="entry name" value="HisA_HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000110">
        <property type="entry name" value="1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011858">
        <property type="entry name" value="His6/HISN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02129">
        <property type="entry name" value="hisA_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090:SF2">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8 12 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4366826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="258" mass="28515" checksum="4F1A434C21DAB9D8" modified="2023-11-08" version="1">MTRFRPCIDLHAGSVKQIVGGTLNTTTPDVLKTNWTSEHPSAYYADLYKQHDLTGAHVIMLGPGNDTAAKEALEAWRGGMQVGGGIKEKNAKQWIENGASKVIITSYLFPDSTFSMDRLRAVLHALDNDKQKLVIDLSCRRKDDKWFVATNKWQTITDFELNQDSISLLEPHCSEFLIHAADVEGLQRGIDHELVTKLAEWCSIPVTYAGGGRSLEDLELVKKLSGGKVDLTIGSALDVFGGSGVKFEECVGWNKEQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6J7</accession>
    <name>A0A9W8Y6J7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3-oxoacyl-[acyl-carrier-protein] reductase</fullName>
            <ecNumber evidence="2">1.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_005330</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 8">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6J7"/>
    <dbReference type="OrthoDB" id="1669814at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050259">
        <property type="entry name" value="SDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879:SF2">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE FABG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4369568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="259" mass="27400" checksum="B82078421E0F3DAB" modified="2023-11-08" version="1">MSNIQNRVIVVTGCSSGIGLATARLFLERQAIVFGIDVGAVPQKLADTHSTFTFHQADLTSDQAVEHAIAKCIGKHNRIDVLVNCAGVSDGWSSADTIQDEEWERVICINLTVPIRMMRAVLPAMKEQKGGSIINVASKAGVSGASAGIAYTSSKHGLVGATKNVAWRFHTDNIRCNAVLPGGVATNIASSVQMEHFDSAGFNSFFPTVQLHVSKDSEGTPVPVIGVDDVARGIAFLASDDAKMINGALIPIDNAWSTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6Z0</accession>
    <name>A0A9W8Y6Z0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER-golgi trafficking TRAPP I complex 85 kDa subunit-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006477</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6Z0"/>
    <dbReference type="OrthoDB" id="203724at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024420">
        <property type="entry name" value="TRAPP_III_complex_Trs85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12975:SF6">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12975">
        <property type="entry name" value="TRANSPORT PROTEIN TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12739">
        <property type="entry name" value="TRAPPC-Trs85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="710"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="718"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="761"/>
            <end position="774"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="774" mass="86077" checksum="F0638E06D646A38B" modified="2023-11-08" version="1">MTPPQDATPKPPKYPADLEASMILPRQRASPASSIASLPYRRSNPSLSKLFESTTSISTSRPSSGTATPTPSAVPGSVFSPGSRAGGTGTPSSLPLGVSDEYRVLIQRAFVPTVAVLADAQTEELIRAKGVEGGLVQLLRPFGESVPGKVTIRDSNGASKSWEDFGVRFAGVDDAYPDLRVDTAGQRTSSSSMRRPRRTGGDVAQIEELVDRHLQYSEFNRQNPVSDYLNRGEPVALTESTSSPFYTLYLRRLLSGLPVVPHETFSHPVAGIIAISSRNPHPIEELRRLYNRQHDGDLRFPQWVENDFLRYYVLIHDEETGDIAKSNQTFDSMKRHFGLHCHLLRLKSQQCISSDDDSVRLPTCEWMSASEELAEIQRREATDDISDPTPYLPDSDISSLRTFIRELVTQSVIPNMERSVSTWNEQILSRRRGLSGRFMSLSKRWTPFGASRNSSASSTSSSNSNYDSLQGFYRPDAPEAIMRRLADYCFMLRDWKLALSTYEILRTDFQNDKAWRHYAGAAEMAALSALMAPTPMTSKSRSENIDAWIEAASYSYTDRQRSAAPYYALRTLALSFELLRLRGSSAADDAARWAIRILDTGLVGPIGHALITERVSACYSIRTGVGAYKLGNRRRKAALWAVLSAENWWRLDKSLPAEKCLDTALRLYTVQGGNEEGGAVKLPFEDMQYFVDELRQQIMNVRLSNKGYTADEARQEDEGMQSPDVEEATETLDTSPRTHRKSLIGTAAPPIDVGPLSPALERNEEADLKSEGFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7J0</accession>
    <name>A0A9W8Y7J0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_005521</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7J0"/>
    <dbReference type="OrthoDB" id="10253869at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031956">
        <property type="term" value="F:medium-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04433">
        <property type="entry name" value="AFD_class_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201:SF5">
        <property type="entry name" value="MEDIUM-CHAIN ACYL-COA LIGASE ACSF2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="90"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="NAD(P)-binding" evidence="6">
        <location>
            <begin position="556"/>
            <end position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4369757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="812" mass="89058" checksum="5114015E80020FCC" modified="2023-11-08" version="1">MLAPKITTSTYNGFLEAEVSGGPLSNADSIFKHLETGLQRNPHSPAVICMHQAAGHLSEWLKSGKKTSELSNMNPVPLSEGQLTGSYKDLRDALHSLKPSIVVVPDQISAVPVDIAVKELCIARPFGIILSGESSDSEWCAFSSFSETDLSVEFEQTLTDQARHDDPQRIHSMMFTSGTSGNPKGCPLRVHGQTHFLESWSWLLNEQNSLRSLQQAHNSRAIAPTQMLQTWHTGGTVLLPSRSFHIEDTIAAIDTFGATFVVLSPAMVHAITDVIQSRSHIDLSSVRTIQIGGDAVNKTVLLKCAALFPEAQICTHHGMSEGVAAFKWPFFDISPSKIPFLGEICPTGTVAAGSTVRLWHADEQRTVARNELGDMHIRSSSIIHHYLGGKSEESFYDDPEGRWFITGDKAIMDSTGMVYILGRSKEAITRAGLTIMPGPMESLIQQFLGAQTCVVGIPDARFGEKTYAVVKAFKGKTVDDIVKHVHGFLGIQYELDGVLTLEELKLAEFPVNQTFKIVKRSCSLPLLASLTRILSSTRSLHKTKISYMMSTYAVLGSTGNCGTALIENLLKSDAASKINAYCRNKNKLIRLLPQTNDDKRVKVFEGSIADVDIMASCIKDCKAVFMVVTSNDNIPGCTLGQDQAKSVISALERLKSEAAPGTLQMPKLVLLSSATIDDHLARRMPWWFRPIMLTAGSNVYHDLRMVENFLRAQQDWVSTIFIKPGGLSVDIQRGHKLNLDHEESFISYLDLAAAMIEAADCAEGIYDMKNVSVGNANGRARFPPGTPKCILMGLLRHFFPFMHPYLPTTGPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7M9</accession>
    <name>A0A9W8Y7M9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006306</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7M9"/>
    <dbReference type="OrthoDB" id="3685192at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4369221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="269" mass="30399" checksum="655641672BB8629C" modified="2023-11-08" version="1">MAAADLFAYQVLGPPVDAIRLLTLLPAQFSYDVYLSITHEELEDKKQKSNIDQPFSTDSITQAVISKVSLNNHLYSTMAPTFKEFLSAYPERPRGKKSVAFTDAPPEIHEIPFEYSSWQNGEQAEPPSAFEVDHAEMLKEQALAAATAEELADAKEQAVQEANMHLDPALTQNFSCSIKRKPLPVRPKLMRELRTTPDRRAEEPFTPPMLARANTTFDDFLPDHKLNTWQNVDDAARQRRLKHKVSLLAADTLLKAQHHVKKAVVQLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7T9</accession>
    <name>A0A9W8Y7T9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_005713</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7T9"/>
    <dbReference type="OrthoDB" id="5428495at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026030">
        <property type="entry name" value="Pur-cyt_permease_Fcy2/21/22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806">
        <property type="entry name" value="PURINE-CYTOSINE PERMEASE FCY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806:SF5">
        <property type="entry name" value="PURINE-CYTOSINE PERMEASE FCY21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002744">
        <property type="entry name" value="Pur-cyt_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="185"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="242"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="342"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="407"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="450"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="487"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR002744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4369949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="513" mass="55400" checksum="64596C258F980872" modified="2023-11-08" version="1">MGARKDVEDGRFPSKDQTKQSKAEEDGRHEVGGAGKQTRLGGGWRGVLNLLEKRGDVELRGCTPVPYDQRNETNYFNIFTLWFCVSCNPLPVTFGMVGTMGFGLNLRDAALVILFFTLISAAPVAYLCTWGPMTGMRQLVQARFSFGKYLVSVLVLLNLATLTGFCVIDSIIGGSALSAVTDGETISATVGIVIVCLLALVISFCGFRVLHIYERWAWIPALVALIIAAGCGGKSLSQQVEAPAATAAQVLSFGGLVASFMLPWAALASDFSTYMHPMAPRHRIALYTYTGLSLPTILLMTLGAAMGGATPNITEWQAGYDSNAASGVLAAMLHPAGGFGRFVTVILAFSMLGNLSATMYSVTLNLQMFIPWLFRVPRVLFSIIITGIIIGVAVEAAKSFFLNLENFVGVIGYWSSAFIGIVITEHLLFRRATFASYTEDELAWDDMKKLPPGFAAIGAAVLCFGLVIPSMSQTWFTGPIAEHTGDIGFEIAIVLSALLYVPLRYLEKKVWGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y841</accession>
    <name>A0A9W8Y841_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Autophagy-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ATG8</name>
        <name evidence="11" type="ORF">N0V83_005550</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Autophagosome membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Vacuole membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ATG8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y841"/>
    <dbReference type="SMR" id="A0A9W8Y841"/>
    <dbReference type="OrthoDB" id="6738456at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16128">
        <property type="entry name" value="Ubl_ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000010">
        <property type="entry name" value="Autophagy-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0072">Autophagy</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="lipid moiety-binding region" description="Phosphatidylserine amidated glycine; alternate" evidence="9">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR604241-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4369786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="119" mass="13876" checksum="38F61030E25A1E48" modified="2023-11-08" version="1">MRSKFKDEHPFEKRKAEAERIRQKYNDRIPVICEKVEKSDIATIDKKKYLVPADLTVGQFVYVIRKRIKLSPEKAIFIFVDEVLPPTAALMSSIYEEHKDEDGFLYITYSGENTFGEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8V4</accession>
    <name>A0A9W8Y8V4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_004605</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8V4"/>
    <dbReference type="OrthoDB" id="5981550at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000003">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.130.10">
        <property type="entry name" value="Ubiquitin-protein ligase E3A, N-terminal zinc-binding domain (AZUL)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032353">
        <property type="entry name" value="AZUL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042556">
        <property type="entry name" value="AZUL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044611">
        <property type="entry name" value="E3A/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700:SF8">
        <property type="entry name" value="HECT-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16558">
        <property type="entry name" value="AZUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="7">
        <location>
            <begin position="851"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="500"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1016"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="513"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1017"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="5">
        <location>
            <position position="1177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4371388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1209" mass="137769" checksum="16988BA211636281" modified="2023-11-08" version="1">MSTQNRGHASRRGSRASSSSGPAGAGRPSRRLAPPFSNVVDVQDPNRVLECAQLERQLRFQRLVRQYLSQILHGCKSAYCTTPTCFSCNKRNAPKPHRPPTQLTARALAHYLASQDNPHRALCPHELKVPPDSFEIEGAIGTFIQQRRNGTDRQYSVYPAVRTLPLQYVHTEKGGHAIENTYPVASSIEQSPGIQQRVIDALGERHQTKKDSKSLGQNLYDSAAMISYFSKQIPSPASVLALIRSPSNVPHHGKGTPQPIAPSVMPPETDTDRIVRQHALQNLWPHGTLQATQIASETLGNGQQILKIPYHPPSATKPSQIPTEPTTLDDTLELPRLSIKKTGRKNFTIGGEEPRIPRQNKPALQRAQSVSKHVPHTDPVVPVVAKLNCDMLDEFLEDASQQHQTQITLDLDMTLGRFSRGYRTKAAVRRSLFYSLSDPEKLLVSFHDSNDAFSHSPLPHIDSARLVKSMRHWVWETEALIFDSLYIAVEALFTPPPELDVQKSPRLKPSRKISPTDSCSRQSPDQQTFRNPTSRYLSTDEAAHIVMICIHALTSYIPWGSAFEWAPLRSLRSRGVNETRIPWRDGTSPYTYTYLTDRLEYEPAIRLADRLLCGIGARTCFEHILASLSRRDKREEDLGKTSADNTLLHTITQHLKVVERVALDHKNKTDFTEDTSEDPGWTVTATFMEWARTIIINKWENKAEINKWSSVGTAVMLFDKLHIDYGPLNLRPSMFELQIFHDGLDTVEEPLRFLNWEEKPNVLHILQYPNLFTAQQLVTYFRTINFTSMMRQYDHTTHVHQMHRCLDMFLPRPYSWVINHRMKTTLSDYLVLNVSRENPLKDTLDQLWGQEKRMLLKPLKVKMGQQEGEVGLDHGGVTYEFFRVVLVEAFKPENGMFTFDHKTHMTWFQPCTLEPEWKFEMLGILFSLAVYNGITLPVTFPLALYRFLLPADSTFRNREIQPDTLNSIKDGWPGLAKAFQELLAWSDGDVGDVMMREYAFSYEVYGQRIDHDMRQPFSHNASGRRNANSGNADEDEPSLVTNENREQFVRDYIRHLTFESVAPQLGAFLKGFLTCIHPRSLYFFTPTALRNLVEGTQHISWSDLMECARYDDGYSPMHQTILDFWTIVEEFDQEDCRHLLEFVTASDRVPVTGYESITFDIVRVGGGPENLPTSSTCFGKLYLPDYPDRETMSRKLKLAIQNSQGFGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9C8</accession>
    <name>A0A9W8Y9C8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_004551</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9C8"/>
    <dbReference type="OrthoDB" id="5392779at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47840:SF1">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47840">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4371334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="663" mass="74223" checksum="0D95D1EC9686BA82" modified="2023-11-08" version="1">MTDSGSMASHEGGRKRPPKGLRRACWECKRPKIQADSSQTDISSPNLSAFGPDRVDQNHACSIHIGERLSKLEQLFERFVCRKPSTAGTPVDAPRSPTLTSSSDMEPKFDLPKFSSDTQSMSSLGDGILGAQTWTSAPSINTLVDKPDQGHPSSSDKVHRALTALLPSQHDADIIFESSNGWMILDGIYSASPDIYINKDHQSYALDMQAIARERSIIVARTLLHLAICINSLPPEFDDSRLSSMWNLDATIQNYVATVTSLVTSSDEQMLTLHGLETLLLLALYHLNSASLRQSWLIIRRGMNLAHLMGFQRQINQPDLQPPIPAVSNAKQIWRTFVDLDRYLGLHLRLPFGADDYPLPEDANLHAVHRSKLNVISRQIAELDREVSPQAYAAALALDEKLESTMKEMPKDFWEVPNIPSTARSPESFATLERLMVQMWHFEMKIFIHLPYLLRAPQEGRYEFSKVTALQASRNVLMRWFALRNAGITQACCRFAEIGVFIAAVTLTLDIVIELGTKEKSEVQKTKGTDFAMLCRLIGEMEKLAKASSREKIAARSAVVLKKILSSLDPSRQSAGKTRLSIPYFGTIELEFKKLPRRPAFDVDSETGKMMNAKATGQHLPVFSFISNALWPPTGNDSEYDFDWDIVLFDGLEDRDTEGNWVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9I1</accession>
    <name>A0A9W8Y9I1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_005309</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9I1"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4370787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="248" mass="28002" checksum="0372633B05C69DB9" modified="2023-11-08" version="1">MQAPHIFDLSSLSGSKLTYLFAMGRTYSPMTDELSYLLRRSMEKSTFDKTPRSEFLPKGKLDQLITKESILAEFDLDELCDEDEVLVAFILDKAKSLFATTASASLKAAGPNSLYKAMALFYDHGFDDSNLPIKDRSHESTEAVSTKWLQHPFACMETESGVKKIRHRLWTGSTIYNFHEAQWKFIVPKFSTSEPNHDLGEHIVPFVSKHSSGEGAFGVVWKYEIHNDHINDSSNSVGENKRSLQALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9N8</accession>
    <name>A0A9W8Y9N8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_005410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9N8"/>
    <dbReference type="OrthoDB" id="19653at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4369648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="34357" checksum="AB4DB5F7C2718688" modified="2023-11-08" version="1">MEFQWPDDSMFSHFTIFQEKFKTVGSVDINAAIINPKDITPGSHPVVINLHGGFLMYGHALFAPFFAPGANGVADSLEDLEDFWQWSRNQLPEILERRAPGHSFDFTRLLLTGSSAGGYCAVQVALSHSDEVSAMAMAYPFVDPADDVIVNGPTGTDPNIFRSPFESLPSKEAVMSWIEEQKKTVKVQAGMETTPFAVAATQYGLLYSHIFDSRNLRRSEFLPLKRLQLGATLPQNIWILTGEDDSIVYFRTIQKFVDLAERNPVQSTIRLDIAPGEDHAFDLTKTTWELHAIGAMDFVKKSWLHSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9R6</accession>
    <name>A0A9W8Y9R6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">diacylglycerol O-acyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">DGA1</name>
        <name evidence="18" type="ORF">N0V83_004947</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>an acyl-CoA + a 1,2-diacyl-sn-glycerol = a triacyl-sn-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:10868"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:64615"/>
            <dbReference type="EC" id="2.3.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycerolipid metabolism; triacylglycerol biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the diacylglycerol acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.20" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9R6"/>
    <dbReference type="OrthoDB" id="264532at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07987">
        <property type="entry name" value="LPLAT_MGAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007130">
        <property type="entry name" value="DAGAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317:SF0">
        <property type="entry name" value="ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03982">
        <property type="entry name" value="DAGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 18" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="190"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="125"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="33"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4370429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="508" mass="56869" checksum="BF69E10162BD37A0" modified="2023-11-08" version="1">MSLNPEPSAPDERAVQDLPPKSYADAAEEALEPDSHAANHTDGTLEHAGQRQRQRQRAKADQVRPNGIRIPSADDKDSLEGVGMDSSPKSPTVKGHRRVASRSSHGSLGRKHGEHVAHELYEKHQNGNGDALTSVKPPQELDRVGRTDALKRRNSELKSGRQAGAGWARSKIRFAPMNVPLQRRLQTLAVLMHTLSIVGGLAIFFFLCSIPLLWPILLPYTVYVLFSNAGTSGELSLRSERLRRLPVWSLFASYFPARLHRSQELEPTRKYIFGYHPHGIISHGAFAAFATEALGFSQLFPGITNALLTLDSNFRYPIYREYALRLGMASVSRESCENLLSKGGPNGEGMGRAITIVVGGARESLDARPGTVRLVLRRRKGFVKMAIRTGADLVPVLAFGENDVYDQLDGKSHPYVHKFQMLVKKVMGFTIPIFHARGIFNYDVGMMPYRRPINIVVGRPIRVVQNKHPDTEYLDQVHDQYINELMRIWNEHKDTFAKHRTGELEIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9V2</accession>
    <name>A0A9W8Y9V2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004824</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9V2"/>
    <dbReference type="OrthoDB" id="2270193at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057654">
        <property type="entry name" value="Znf-CCCH_tandem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37543">
        <property type="entry name" value="CCCH ZINC FINGER DNA BINDING PROTEIN (AFU_ORTHOLOGUE AFUA_5G12760)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37543:SF1">
        <property type="entry name" value="CCCH ZINC FINGER DNA BINDING PROTEIN (AFU_ORTHOLOGUE AFUA_5G12760)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25542">
        <property type="entry name" value="zf-CCCH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25543">
        <property type="entry name" value="zf-CCCH_tandem"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C3H1-type" evidence="1">
        <location>
            <begin position="55"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Tandem CCCH zinc finger" evidence="2">
        <location>
            <begin position="91"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="20186" checksum="98A7266F6F009121" modified="2023-11-08" version="1">MEPSPGSVVNLDRSNISANLPTTAMPGFIPLNKDGQRLDTYTHPPTQEEWRIYNARFHKQKPCNSFHLQRICTTFGCPFDHSELEPESRHVLEYVLKCSPCPRKSECRAADCYYGHICHKDDCTGQMKGCRMKADLHSVDPKVARMVAAAAATEEELEEEMVVDAPLVDMPDETNFLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9Y2</accession>
    <name>A0A9W8Y9Y2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_005367</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9Y2"/>
    <dbReference type="OrthoDB" id="6161812at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4369605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="71" mass="8342" checksum="BD9ABA22B06D0C25" modified="2023-11-08" version="1">MSTFRIMTHRKPEHARALFKKADFLRSLGSDDYKDLLDEATKLTKEAKPELESWKQLGLKDFDDVVSIWSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA16</accession>
    <name>A0A9W8YA16_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_004776</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA16"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4371557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="402" mass="43920" checksum="C30C9B625DE3BF0E" modified="2023-11-08" version="1">MQSKPGEHALLQRPQWRRSVFVSWQPPEQRRTPGPPQMGVGVEVEVGVVEELERVVVFVEEVEEVEEEEDAVLLVGSDEVVLLAGELEVLKGEIVVVVLLEDSVEVVLDEEYVVLDNEAVLLGTSDEVVLLEFPNGGALVDGVYVLENEEVLLLEDSDAALVLLDVSDEVTLLEELDSLEDDAVLLEDAVVEVELLEVVDGSSEEVEGLLEAVDEKVEVTGVVHETLEVMDDSLDVGVMKLEVLGEPPEVDDEVSDVEDWLEVVEVRLEAEEVPVEVSGELLEPVVEMLEVVDGLLEMPDELLWEDELLDSTEDVADVPVMLDELSLLAELVAALDDTEALVSVLLKLLLDCEVVARVVSELKDVCVEVKVVGQGVPVVSVSVMRVDVVVVVVCIVLALMFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA70</accession>
    <name>A0A9W8YA70_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005682</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA70"/>
    <dbReference type="OrthoDB" id="77989at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03426">
        <property type="entry name" value="NUDIX_CoAse_Nudt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045121">
        <property type="entry name" value="CoAse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992">
        <property type="entry name" value="NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992:SF44">
        <property type="entry name" value="NUDIX HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="82"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="468" mass="51178" checksum="E90D0A536261A570" modified="2023-11-08" version="1">MAIPKDTEDIITHLKSVLLELNAHPYPEIACPPGVPKRASVALILRIQPNYSHWPAFSDKPLPTLARSPSVRRRSSAASTASHKFNFEAILDAFFEQDWVKHGDPEVLFIKRATRVGDKWNGHVALPGGKRDPEDDDDAVTAMREALEEVGIDLGYNNAIAVGNLPQRIVTTSWGKVPLMVLCPYIYLLTSPSYPAQRLQPTEVASSHWIPIRALLSPDLRTHEYADVSARLAKSELGIKRWFLQAMLSKMMFAAIQLVPTNSTYCATIPDFIPASDGATSLMGKAKAALGGSEARYADRKPPLLLWGLTLGVVSDFLEHIPPHNALELWTYPTFTNWDVRFIMWAMSYRFRNQKAMEMATTTTSSSITGTSCDVPVQDLAGAGAEALGGLSEETEKPGEVGIAGLGVGRGWGQTGRAKMIARGAAVNSMLEGYYPIVRKAVATALVGRLSVAALLVVWAWKSLKKNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAH7</accession>
    <name>A0A9W8YAH7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003863</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAH7"/>
    <dbReference type="OrthoDB" id="5415512at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="104"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="314" mass="32936" checksum="5813787B0AFF366D" modified="2023-11-08" version="1">MANEEQRKAFEAYMGNASASNSQGQPIPQYPGSVANPGNPGNAYPYGVYQFPGLGAPPAAPTPPLAAPSSIASYPSYQSGAPQPGYGHGGYGQGGFHPRPPPLGQAAAPQHQQQQMSPPARPPGYVQVPRSYNAQPPPPPGYDVGWQYTDPMAEQNRRLSGAPAGAIAPTSRNTSGDIVESQSRQTNEYYHYQQYGRPSAPPAPPAPSAPAPVVEEIPVPVITAIIQLCQASPWCQLHVDVARRRSGLGDIRVSLAYALVPPMNLVTGLGSLFTSTLIIMSTGAGDEIEKKSMSPGTLPVGLGLGEAQARHTWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAI9</accession>
    <name>A0A9W8YAI9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAI9"/>
    <dbReference type="OrthoDB" id="3509362at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08276">
        <property type="entry name" value="MDR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052711">
        <property type="entry name" value="Zinc_ADH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45033">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45033:SF1">
        <property type="entry name" value="OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="1">
        <location>
            <begin position="16"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="370" mass="39867" checksum="C7BF8A0B1CA63747" modified="2023-11-08" version="1">MTSPQKVKQWLTGQDGLDKLKTTEVDCPVPGEDEVLVEIHTVSLNYRDTEVCMGLYNHHKSVEQPPAIVPCSDMCGIVVAVGPTSSNTSDPYKNTAFRLQTGDRVISTFAPSHLTGQIQAKDLGNGLGGPAPGVLAQYRIFPTFGVVKVPEYMSDAEASCLPIAAMTAWMSLNCMRPNGEIVSKGETIVCQGTGGVSISAAQIAAAAGADVIVTSSSDEKLQRAKSLGARETINYRSNPDWENSVLSATKDQGADIIIEVGGASTLRRSFDCVRFGGLIACIGYLGGKQDQEADRTNTNLLCLKRNVTLKGILNGPRDELERMIAFYERNKIKPVVDKEFSFEEADQALKYLFSAGHFGKVVVRVKASGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAS2</accession>
    <name>A0A9W8YAS2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vezatin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">N0V83_003815</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the vezatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAS2"/>
    <dbReference type="OrthoDB" id="21151at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017022">
        <property type="term" value="F:myosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026859">
        <property type="entry name" value="Myosin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026858">
        <property type="entry name" value="Vezatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15989">
        <property type="entry name" value="VEZATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15989:SF5">
        <property type="entry name" value="VEZATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12632">
        <property type="entry name" value="Vezatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin-binding" evidence="16">
        <location>
            <begin position="133"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="472"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="507"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="397"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="509"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF12632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4372042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="594" mass="65519" checksum="186859F145064DE4" modified="2023-11-08" version="1">MLGEGRGQDSRPASPVASSPDKQGFAPRGPSTLQSRIRDKLPQPLRIHERHGSIARIHTACSKAVNSRLGRADNERFLEHFRYLIVASQLLGEQGGLRTAAIPSFTANGPPGPQEFKIATISPTGAALTATTAFILVWLLHWSQRCGGFSKGRSLVVVVVFAAVATTVYTYMRRQWLQYLRQESVEGASALVTNLQSFEASTSSALALIQEVELVSRGYRLSSPLPPISRLEEKGQARRCQRLRRSLRAAFADIIPILADQCGTLKQLIQEDDLEKYLDVYEISNPDLQEAAQGYSATEFEDAETLKALRTLQYRLSTLRRVYLCSLLALEADGGKPDFIRWSTVVDSMLSLARPVGQWSENFNSVLAEEEQFILPSPNKVSHTPGREKLRNSVRKLSGLSQGIRGLQAKMQVLREETNKTLEDTEDVTELGQHLMAQYESIGADLKSLMQAWEAGKSALALNIDRHERRISQASSGLRSPVPSLGGLTAVDEGSPSDALRALNGDLLSPQQSAPPSDQGSTSDDEVFEAIAIPKTRVTMSREERMHKIQEDRARQASLREQREASTNMLRELESVINLRPTRVTGPGARVTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAZ3</accession>
    <name>A0A9W8YAZ3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mannosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ECM39</name>
        <name evidence="13" type="ORF">N0V83_003973</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the eighth mannose residue in an alpha-1,6 linkage onto Man(7)GlcNAc(2)-PP-dolichol to produce Man(8)GlcNAc(2)-PP-dolichol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29535"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="Rhea" id="RHEA-COMP:19518"/>
            <dbReference type="Rhea" id="RHEA-COMP:19519"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:132517"/>
            <dbReference type="ChEBI" id="CHEBI:132519"/>
            <dbReference type="EC" id="2.4.1.260"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:29536"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyltransferase 22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAZ3"/>
    <dbReference type="OrthoDB" id="19039at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052917">
        <property type="term" value="F:dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF1">
        <property type="entry name" value="DOL-P-MAN:MAN(7)GLCNAC(2)-PP-DOL ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 12 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="95"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="222"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="272"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4372199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="481" mass="54286" checksum="B753E86382898610" modified="2023-11-08" version="1">MTSLAWPFYLLIPTFILLHLFISPYTKVEESFNIQAIHDILIHGIPTENVDQFLTSNYDHVSFPGSVPRNLALRSLLNAYALPWNRQDVSRGYRLSLYLLTIAGIVFRSELAILVGTITLYLFLTQRISIISVIIPAGLGGLIIGLMCTVPLDSFLWQSFPLWPEWTSFYYNTIQGHSADWGVSSWHYYFSNALPRLLLNPAIYLLCIPVAVLNAATRRRSLDLLIPLLGFVALYSFLPHKEWRFIIYVVPGLTAIAGTGASWIWTRRAKSVIYAAISLALVASVLVSFAASTAILGISSLNYPGGEALKVLHNEIEHPPKRHFDVYFDNLACQTGVTRFLESHNGPQTVLDVLEAQDSLQKRTWAYDKTEDPTMLLDPMFWAKFDYVLAERPEKVIGSWAVVHVVYGFDGVRLLKPGEESGSQTEPLYHAKKEVADGSWASKVANIWHVLEGLLRDSILRGHWAEIKMEPKIRILENQMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBJ3</accession>
    <name>A0A9W8YBJ3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxymethylenebutenolidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003663</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBJ3"/>
    <dbReference type="OrthoDB" id="5440at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030638">
        <property type="term" value="P:polyketide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009959">
        <property type="entry name" value="Cyclase_SnoaL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38436:SF3">
        <property type="entry name" value="CARBOXYMETHYLENEBUTENOLIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38436">
        <property type="entry name" value="POLYKETIDE CYCLASE SNOAL-LIKE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4373368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="19887" checksum="10BFBA85343E7CA1" modified="2023-11-08" version="1">MKAGKNVEKLLETYIPGPDGPTPFVNFTPTMTGGHSLPELRRFYKDYFLRAAPPSLHMRLISRTIGVDRIVDELYIQFKHTCHMPWILPGINPTNQKVEIVVVSIVAFRAGKVWCERVYWDQASVLFQVGLLDPEEVPDELKKDGLEMLPVSGSEAARKVMDVQSEEANDMIDDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBT5</accession>
    <name>A0A9W8YBT5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_003202</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBT5"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015203">
        <property type="term" value="F:polyamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010509">
        <property type="term" value="P:intracellular polyamine homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF5">
        <property type="entry name" value="QUINIDINE RESISTANCE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="294"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="477"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="501"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="543"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="566"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="138"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="65"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4372911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="603" mass="66661" checksum="01B5A543CF713E99" modified="2023-11-08" version="1">MANPGNTPSRPGTTRTEHEPGRSYEERVEEKDIAIDKSAVQEKDADMTSTGREKKEEEEEDAISISPSHASTASEQSHHSSASTEQKSRPVTLSRTTTITPDAIIVPRSHRRGLLARFALIPEVTNPEHYARKTKWFLTFIVAFCAMAAPMGSAIVMPVLQEISIAFNATHTVTNMSVAVYMLSMSIFPLWWSSFSERLGRRTIYITSFILFVLFAILSAVSTNIAMLIVVRTLSGGAAASVQAVGAGTIADIWETKERGRAMGIFYLGPLCGPLFAPIIGGALGAGLGWRSTQWFLVIYGGATLVFIIFALPETLHRQEKNDASPVSRPEDLEEQNIGNKTGDAELRRTATRASTIRKTKTVLTHLRHIFIDPLTIVTWLRYPPVFLTVYYASITFGCLYILNISIQSTFSAAPYSFSTLIIGLLYIPNSAGYLLASLFGGRWIDSIMHREARKAGRYDDRGKLIFRPEDRMRENAWLAAILWPSALIMYGWTAEKGVHWIVPMIANFFFGIGSMLIFALVNTMLTEFMPKRAASGIALNNFVRNICSFTGAVVADPILSGIGNGWFMTILGLWSVVTGCLALFAMRKWGERWRGAMVEALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC57</accession>
    <name>A0A9W8YC57_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_002621</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC57"/>
    <dbReference type="OrthoDB" id="19619at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051975">
        <property type="entry name" value="mtLSU_mL45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28554">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L45, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28554:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4373882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="376" mass="42872" checksum="12D61C9D93936E88" modified="2023-11-08" version="1">MSTQAPLRSVRIPALQRQCLFQRRQCLDRSFPYAASRPFSSTALRPYKKKTPRLQDRTAVEASTENYVSPSPYAAQAAAQGDIGQMAEDIGLLQHTIVRAPFKHLPRFTSWEFYDYFWKLLKSKGLGFYSRSSYKTMIQKEGWSRYLPVGAGKNAELKIEAKKMYEEVYRNFAKFVLLHFHTYKAACDIDPFHRGNVDAIRPICLPPVLNQFDARIAARGPLKMDWKLLKWRSARVVSHRATPLGEDQPDTAYRQAIIRLESTQSLSIAEPHQPSASPLSRRGNKAPSGLAWIPEEAKAKNVHAVQQEEGGFTDEFLDNGKQKTVVEYLVLQMRVIRGKEEKWKVWGFANESTPRTIEEDDIYWSKMLSVQAAGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCM5</accession>
    <name>A0A9W8YCM5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004779</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ornithine cyclodeaminase/mu-crystallin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCM5"/>
    <dbReference type="OrthoDB" id="41492at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000577">
        <property type="entry name" value="Proline utilization protein PrnX, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="entry name" value="ornithine cyclodeaminase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812:SF19">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="89"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="486" mass="53850" checksum="38F79E9FB71C6726" modified="2023-11-08" version="1">MPLTILTDDDVRGLLLQLTKQDILDLQQSLADALHYYSTSTEEESNGCCSSYQPLRTALKRNDGQTTLLMPASSNDGIGVKIVTLATPDKRTPSDTSSLTSSVDTLSLSQQSSPSTTSSTTGHEPPKSLASAQTTTPKGSLTLFDRSGNPRALINAEEITAFRTALASTMLFKKRENVHDVVVFGSGKQAYWHIRLALMLRGTDIHHLNIINRDFERVHHLLQKLYNPHEQPHNWSESTGIPNYREKFLASDLHRPKIQILTPGHGEYERLLKSTIRASSVIFFTTPSLTPLFPAPYLTNPEGRKKGRYLAAIGSYKPHMVEIHPDILRQNVAPEHGHRHFHKHAQQAGAVVVDSVEACLKEAGEIIQAGLGPNEVVEIGELLMLKRDAERRRRECQASGENKSAEGLDEGGVELGECEKAKKKRRGSREKDHEVEDKAHKSLMDWLQKGNVIYKSVGLGLMDVVVGGDLVRLADERGIGTRVENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCV3</accession>
    <name>A0A9W8YCV3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_002537</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCV3"/>
    <dbReference type="OrthoDB" id="4685598at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053181">
        <property type="entry name" value="EcdB-like_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47785">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="563"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="48"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="640"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4373798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="667" mass="74932" checksum="970F75D7E9A907CF" modified="2023-11-08" version="1">MAEKRKYDRSPGSDLSRPLPDDSAPSSASPQEAPTIVENYPRKRIAIACNVCRFRKTRCDAQKPSCGFCTDLGIECTYRKPTVGDRSKPAGPPSEAFANIERRLAQLEAKLESTQQLVQNSAVLSPNASNHSEIPSSQKPRSRHSSMNSNAANLVKNITMSVRSVGTPVPYDFAYRQTSTEGTTPRPSLTTFRAPSYIHVESWDYSADFYDDELQAGDQLADQCDECAHRPIDLSRRMCRRLQQSFVENFLRWTPIFDQHACVDALEQAETAAFAYSTPPVCLAFMILALGAISEGRNESPNNLTPGLEYFARGCQILERLSLRTGNLTTLQCRILQASYFKFAIRPLQTWNSITSAARDCMHILSSQATRRFNPTEQEAFNRAFWACSTILHEVEATSKMHPTGLRHFHDLVPLPQFEEEDSGFYFFLAQISLRKFLTQTLEVVGYLTGRVIFAPVVIGELRKQVREWYDHLPSVVRFPLDASPLFDSRKSFLRIQYIALHVVFGWPSVLKILENSEGDPTIPDQETLRVTTEQANSCIRSSALLMSIADEQLMGRKMGTHFTLYATFACFATLLIAFNHPALAFAEETRQEQPIRNGYEVLRPWAHLPLIRRALERGRILMQQAGLAHIFHAHADESPPASMGISPGVASSHDWTSPHNGIPMKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDA5</accession>
    <name>A0A9W8YDA5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA ligase D 3'-phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002725</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDA5"/>
    <dbReference type="OrthoDB" id="2588098at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014144">
        <property type="entry name" value="LigD_PE_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39465:SF1">
        <property type="entry name" value="DNA LIGASE D 3'-PHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39465">
        <property type="entry name" value="DNA LIGASE D, 3'-PHOSPHOESTERASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13298">
        <property type="entry name" value="LigD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA ligase D 3'-phosphoesterase" evidence="2">
        <location>
            <begin position="116"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="451" mass="49929" checksum="3CDACE6EC5CA9213" modified="2023-11-08" version="1">MSIPPHGLTTMDLPSSLARDISPPRPLKRRRTPGNTSPSAISTEEECKKPPPSIEPTLAAVEAGKAKIDDHLAYFKAHLAKALRSTGPETPRLSISDFSALYQDNQKETGHHFVIHQHNHPRAGVHYDLRLQFSELSSMSFAVPKGLPGDPNSRSMGRMAIETRIHNYWNHLIESASAKTGSLLIWDTGTYEVLPRKMGNKSGKGMPSPVATDNENSSSDSEDDGRKSTTASTQQQSRPNENAKLISAFQTRYMRLRLHGTRLPKNYTIILRLPSNEIIKRSPARRKKKSRQKQTQNAHSHNTDSDSEPEPDFDLITIQAVENHKQEEEEEDLNTDSEEDAQTRATNAYPGSTNSIGSIHQRHWFLQLDRQNSGFVLLPIDKNYSSSGGGDSKGKWVCGGKDGGGGFDAFLVRGRDFERSVVTGRLAREVESDEGVGEGFVGRGGWVGVDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDH6</accession>
    <name>A0A9W8YDH6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">G protein-coupled receptor GPR1/2/3 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_002869</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDH6"/>
    <dbReference type="OrthoDB" id="100006at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022596">
        <property type="entry name" value="GPR1/2/3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF37">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR GPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11970">
        <property type="entry name" value="GPR_Gpa2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="G protein-coupled receptor GPR1/2/3 C-terminal" evidence="7">
        <location>
            <begin position="225"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="365"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4374128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="420" mass="47862" checksum="F0F47E2211EACC95" modified="2023-11-08" version="1">MVNLEHLDVDSTSFVLPDHIVPWLRAVVSLGFISFFASVSLLFILTYKIVSWQIKSKRSNQFVILIFNLLWADIQQALAFLLNVEWLRLGSLEVQNPVCFAQGWLVSTGDLGSGVWCFVIGLHTFASVILNFRLRPRYFYLTIFLLWAFILGVSSIGLGLHHENFYVRSGVWCWIHRDLKDLRLWTHYVWIFIFEFGNVLTYALIYTILIHRIRHGYYNIEQAKRVKSISNLMVAYPLVYVVCTIPLASARMASMSGHPPSLARLCLCGAMITSNGWLDVLLYTCTRRIMIFSDEPPPDNNGLDTFAMFWAEKPKRFGGECTIEAMRSSHTKSRRGRSGVTLSSGGSESIDDLCGIGSRDIKLVRTTQVTSEPAQPEDYEEMEAEARKARPRSPMGRWSDETNESRNLSLKELGIVHFPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDJ3</accession>
    <name>A0A9W8YDJ3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADP-dependent mannitol dehydrogenase</fullName>
            <ecNumber evidence="4">1.1.1.138</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_001794</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.138" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDJ3"/>
    <dbReference type="OrthoDB" id="1888931at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050085">
        <property type="term" value="F:mannitol 2-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019594">
        <property type="term" value="P:mannitol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05352">
        <property type="entry name" value="MDH-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000090">
        <property type="entry name" value="NADP-dependent mannitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF12">
        <property type="entry name" value="OXIDOREDUCTASE, SHORT CHAIN DEHYDROGENASE_REDUCTASE FAMILY (AFU_ORTHOLOGUE AFUA_6G13830)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00066645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00069279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4374719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="265" mass="28102" checksum="003E85583C76E631" modified="2023-11-08" version="1">MPLYKPANTDAHVTSQFSLKGKNVAVTGGVRGIGLAASKALAEAGANVAILYSSTKDADEIATKLASETGATVKAYKASVEKKDEITAVLNSIASEFGSLDVVVANAGIASHHPAEDYTEEQWRTIMNVNLDGAFWTAQAAGNIFKKQGHGNLIFTASVSAILVNVPQKQAAYNASKAGVVQLARCLSVEWVDFARVNCISPGFIATDMLSVHPQEWRDKWFGMIPANRLCDPAELKGAYVFLASDASTYMTGANLVIDGGYTLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEH8</accession>
    <name>A0A9W8YEH8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcriptional coactivator p15 (PC4) C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_002030</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transcriptional coactivator PC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEH8"/>
    <dbReference type="OrthoDB" id="2505440at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.31.10">
        <property type="entry name" value="Transcriptional Coactivator Pc4, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003173">
        <property type="entry name" value="PC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009044">
        <property type="entry name" value="ssDNA-bd_transcriptional_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045125">
        <property type="entry name" value="Sub1/Tcp4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13215">
        <property type="entry name" value="RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02229">
        <property type="entry name" value="PC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54447">
        <property type="entry name" value="ssDNA-binding transcriptional regulator domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcriptional coactivator p15 (PC4) C-terminal" evidence="8">
        <location>
            <begin position="70"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="140"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="140"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4374951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="179" mass="19890" checksum="AFBDBFC17BF82642" modified="2023-11-08" version="1">MAGFKRGDFRGGGGAAFKKGYPKKRSSPDDEDSVPRASKKSKGDEEEEEPATVVPKLDVDVDNNHFVALNASGKRRVTISDFNKNTLISIREYWVNDGGELKPGKKGISLTVDQYNALLAAAPLLESVLAKKDIEVVRPDYDADLSAKRETEKDDDKDEEEEEEQAQVNKVEDEDEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YER9</accession>
    <name>A0A9W8YER9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF8212 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002999</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YER9"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058525">
        <property type="entry name" value="DUF8212"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10622">
        <property type="entry name" value="HET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10622:SF12">
        <property type="entry name" value="HET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26640">
        <property type="entry name" value="DUF8212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8212" evidence="1">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="531" mass="60924" checksum="7C47A3AF8786682D" modified="2023-11-08" version="1">MRLIKCSTLELEEFFNIAIDEPRYAILSHTWGAEEVSFQDFVGEKSRRKRRKMTGWEKIRLACKQATEEGIEYVSLISTPRHGQKSFLDVAGLREDELIAPRKVRFYTKEWNLYGTKEGLHQILSKITGIDEDVLGHGWIRFGYLGLREKPASRKMFWAAERKTKREEDIAYCLMGIFEVNMPLLYGEGRERAFIRLQEEIMKDSNDLTLFAWDRSHDGGYTSFDQGIMALSPKDFWRAGNIEWSQSLRFNPEFQMTNKGVRIHTQLRPDTEGNQRYGIGHYFMSLQCHYSSDPAGTVGIELLKGGPQSDHFNRTSEGPVTMKYYGGYLSKASTIYIAKNESFWAQTYIVEDTGLAPPATVKFEDNFPMRRGEKSKWYTGPGSEIFIAFKNEVSEPAHRWENTGPQSSGNFQTIDTRTFIGLRKFRGMCEYERFEFIAVCGFSQASEPWLCLATPESHSAIWEAAEHLDLERVGEMACEVRATSVNLKKDPEHTGRGESMDSIDPTCHIVASSTVDDWDLHVKLKLKTGWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF20</accession>
    <name>A0A9W8YF20_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">DHX16</name>
        <name evidence="12" type="ORF">N0V83_002292</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF20"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000007">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000615">
        <property type="entry name" value="pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF83">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE DHX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="197"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="383"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="813"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="815"/>
            <end position="827"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4375206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="835" mass="94165" checksum="A9B9CADED3F25C57" modified="2023-11-08" version="1">MDPKRRKTDHQDMSELRLASRQKYLAEREAQQLALLRRQVAEEAEEEARLGSKLSERERREFARNRQTLSLAEARQAIDEHLDGYRLPDSDYSNKNEVLTKRHKDKESLKSEVELWEDETLRKVKTQIKRPERVREDDYEFVFDEQTRVSFQTDPATRIDPDKQALQSQLDAAEKAAQTIADTQKSLPVYQYKEVLLQAMEDHQTLIVVGETGSGKSTQIPQYVVENFTKGAVVITQPRRVAAMSVSARVAQEMGARLGKEVGYSVRFDDKTSDETRVKYCTDGILLREVIADPMLSKYSVIMIDEAHERSVSTDLLLCLCRQLVRARADLKLLVLSATINAGKFASYFDQAPILNIPGRTHPVQLNYVQNPEASYLSAAITTVFQIHISQPLPGDILVFLSGEEEILSAMENIESTMKKLRGKAKELIVLPLYSALPPNEQAKVFEPTPSNSRKVILATNIAETSITIDEVKHVIDSGYSKEVTFDPSTGISSLVVSPISRASANQRAGRAGRTSSGFCYRLYTKHAFYNEMSETTEPEIQRIGMDAVVLQLKALQIDNLLEFEFLDPPTPNSIIKSLEGLYALGALDSSGKLTRVGRKMAELPLDIKLSKAILESEKYGCREEILSIVSVLGESASLFMRPKDKRVAADSARQRLAKEAGDFGDFMTYLQIYLQFEESGFDPRWCTENFLQYRSLNRARDVRDQLVKLCEKVELPESSSADHIAIRKTLTAGFFPNAARLQRDGLSYRTLSNNGMTVFIHPSSSLMENRPKFVVYAELILTTKEYIRSVLAINPEWLVEVAPHMHTASAISRLGDDKKMPKERSKTSAGITRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFA7</accession>
    <name>A0A9W8YFA7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">DBP5</name>
        <name evidence="22" type="ORF">N0V83_001466</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">ATP-dependent RNA helicase associated with the nuclear pore complex and essential for mRNA export from the nucleus. May participate in a terminal step of mRNA export through the removal of proteins that accompany mRNA through the nucleopore complex. May also be involved in early transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Associates with the nuclear pore complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the DEAD box helicase family. DDX19/DBP5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFA7"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17963">
        <property type="entry name" value="DEADc_DDX19_DDX25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000849">
        <property type="entry name" value="ATP-dependent RNA helicase DBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 17 22" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 17 22" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="11" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="4 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0811">Translocation</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="21">
        <location>
            <begin position="62"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="95"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="274"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="62"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ4376185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="471" mass="52030" checksum="0BBC786A6FDD7F24" modified="2023-11-08" version="1">MASTEETPAQEPVAQESPAEAQVDGSGIEPGKGSALADTEFNVEVKLADLQADPNNPLFSAKSFEELKLSDELVKGIRHMNFRKPSKIQEKALPLLLMDPPTNMIAQSQSGTGKTAAFSLNILSRINLSNPEPQALALAPSRELARQILGVITHMGQFMEGLTTMAAIPDPSRRGQQFNAQVLVGTPGTVQDMLKRRLIKANNIKILVLDEADNMLDQQGLGDQCTRVKQLLPKDVQTVLFSATFPPNVIAYANKFAPNSNTLTLAHEELTIEGIKQLYIDIDKDNDKYATLLKFYGLMTQASSIIFVRTRRTAEELERRMVTEGHKVAQLSGAMEGPERDRIIDQFRSGEAKVLITTNVLARGIDVQSVTMVINYDVPTMPDGRQADPETYLHRIGRTGRFGRVGVALTFVHDKASWQQLHDIASYFKTDLHPIDTSDWDAVEDMIQTIIKSSRAGKSTQEMTQMITGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFE0</accession>
    <name>A0A9W8YFE0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6536 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFE0"/>
    <dbReference type="OrthoDB" id="5429634at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046623">
        <property type="entry name" value="DUF6536"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35395">
        <property type="entry name" value="DUF6536 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35395:SF1">
        <property type="entry name" value="DUF6536 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20163">
        <property type="entry name" value="DUF6536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="315"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6536" evidence="2">
        <location>
            <begin position="8"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="426" mass="48815" checksum="DF7CC1B63CEC549D" modified="2023-11-08" version="1">MAYKIEEVTGTLLKGSCARVRQLNVWVHLLVNVVSTLLLCASNYCMQVLIAPNRADLDRAHANRHWLHIGVPSPHNLTRIARDRSLLWILLMLSSLPLHLLYNSIVFTNLQANNYVVLPTQESWLYGAPYDFSGFVGFRNETLIPLQFSLSDYIPNLNDTILLNNGTIVPKYKNESTANCFNKYDGQYISEIGNVYLVQSEPTVWRNTTLWGLGVRESTGDFSWYKKYLPPDNHTSLPPVGYNFEDTETIFPFTSTPGTFPSNGWRCPSHRTSTCNVDDMKEVPQNRSKWEPYESPVRYCMVEQVAEICQLRFNFVVAGIVVGANLVKIICIAVMLFWYKKHNALVTLGDALGSFLECPDPETRFRCLQSLSEIKAHWNGGKSRHSKLTKRRVTVEVEARPKRYKSEHRRWAHAPSDSRWFATYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFE2</accession>
    <name>A0A9W8YFE2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alternative oxidase, mitochondrial</fullName>
            <ecNumber evidence="4">1.1.3.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">AOX1</name>
        <name evidence="4" type="ORF">N0V83_002473</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.13" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFE2"/>
    <dbReference type="OrthoDB" id="269227at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047639">
        <property type="term" value="F:alcohol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF119">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE OXIDOREDUCTASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="3">
        <location>
            <begin position="211"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="512"/>
            <end position="513"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4375386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="607" mass="67853" checksum="99B3BB78635E4F04" modified="2023-11-08" version="1">MKLDSKTATFYYSRPSEWLDGRRAVVPCAHILGGGSSINFMMYTRASASDYDDFQAKGWTTKELIPLMKKHETYQRSCNNRDIHGFEGPIKVSFGNYTYPIKEDFLRATESQGIPTTDDLQDLVTGHGAEHWLKWINRDTGRRSDSAHGYIHSTRAVHQNLHLLTSNKVEKVIIEGDRAVGVKLVPTKPLSTGQLQSRVIKARKQIIVSGGTLSSPLILQRSGIGDPEKLRKAGVKPLVDLPGVGLNFQDHYLTFATYRAKPHVESFDDFARGDPAVQERVFQQWNINGTGPLATNGIEAGVKIRPTEEELTQMDSWPCKEFRSGWDSYFKDKPDKPVMHYSVIAGWFGDHMVMPAGKFFTMFHFLEYPFSRGSTHIVSPNPYEAPDFDAGFMNDKRDMAPMVWGYIKSRETARRMDAYAGEVQAMHPFYAYDSPARANDMDLATTNAYALPGNITAGIQHGSWTSPVKKGRAPEPNLLNSNCQEIMDDLTYTEQDIKHVEDWVKRHVETTWHSLGTCSMAPREGNSIVKHGVLDERLNVHGVKGLKVADLSICPDNVGCNTYSTALLIGEKCAMLVGEDLGYSGSALDMKVPNYHAPREVVGLARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFR9</accession>
    <name>A0A9W8YFR9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pre-mRNA-splicing factor cwc26</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CWC26</name>
        <name evidence="3" type="ORF">N0V83_001724</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWC26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFR9"/>
    <dbReference type="OrthoDB" id="6022at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070274">
        <property type="term" value="C:RES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018609">
        <property type="entry name" value="Bud13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051112">
        <property type="entry name" value="CWC26_splicing_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31809">
        <property type="entry name" value="BUD13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31809:SF0">
        <property type="entry name" value="BUD13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09736">
        <property type="entry name" value="Bud13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="16"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="193"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="260"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4376440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="328" mass="36119" checksum="FFD5109482AA8218" modified="2023-11-08" version="1">MSLADYLAKNYLTADSEKKSKKRKRKHKDGGLTIDDDDTLGWKEKAEEDDEDAPMIVGGGTLKKGKKKSKSTSAATWKAVGVAAPSHAEQIAADAAAAEAIIAGTAADRQKAAEAEDEAPEMVDTEGVLRMESGAKAGLQTAAEVVAAMKEKTDKEKREAAKVAKEMGGAAQETIYRDASGRIINVAMKRAEARKKAEEEERKKLETEKAARGDVQNAEAARRKQQLQDAKTMTVARYADDEELNDELKGKSQWNDPAAGFLRKKKAGRSTTGKPLYQGAFQPNRYGIRPGHRWDGVDRGNGFESQWFNSRNKKADIERLEYQWQQDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFV0</accession>
    <name>A0A9W8YFV0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001821</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFV0"/>
    <dbReference type="OrthoDB" id="407617at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="164" mass="18213" checksum="E92D4FE4985A6F2D" modified="2023-11-08" version="1">MAISASVIQVMGLIPPYFELAKRNGRVIGINFWFLTIDYAGAFFSLMAVETGIFLSQAIWLWRVRHIRREAKKVGLMYDQYIAKNPSKKLPRSDSQDSVVDVEACHGHNEPNTYNEKVETTSINNTCTTGLQDSNATSDTTTTTEKETLPKPPPVAVLKSTGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG09</accession>
    <name>A0A9W8YG09_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">rhamnogalacturonan endolyase</fullName>
            <ecNumber evidence="4">4.2.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_001913</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-(1-&gt;4)-alpha-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end.</text>
            <dbReference type="EC" id="4.2.2.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the polysaccharide lyase 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.23" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG09"/>
    <dbReference type="OrthoDB" id="1179585at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102210">
        <property type="term" value="F:rhamnogalacturonan endolyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10316">
        <property type="entry name" value="RGL4_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10320">
        <property type="entry name" value="RGL4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051850">
        <property type="entry name" value="Polysacch_Lyase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029413">
        <property type="entry name" value="RG-lyase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029411">
        <property type="entry name" value="RG-lyase_III"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32018:SF1">
        <property type="entry name" value="RHAMNOGALACTURONAN ENDOLYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32018">
        <property type="entry name" value="RHAMNOGALACTURONATE LYASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14683">
        <property type="entry name" value="CBM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14686">
        <property type="entry name" value="fn3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040837671" description="rhamnogalacturonan endolyase" evidence="10">
        <location>
            <begin position="16"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="Rhamnogalacturonan lyase" evidence="12">
        <location>
            <begin position="311"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Rhamnogalacturonan lyase" evidence="11">
        <location>
            <begin position="406"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4374835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="565" mass="63214" checksum="7C73A8979FD09D14" modified="2023-11-08" version="1">MGVFKALAFAALAVAVPVEERAKKPQSFLKDLGNSTWVIGNEIWNVTQNRQYANKLFYKGGDRVGNAVGHYVSYNGAASDLNWTSAAVADSGNDWINVKFTANEGDFHWVIYDDLPGAYQYFVNHALPTLGEFRTLWRLDNTSFSRGRTNVKDGVLPPLEEYRSAINVQDETWQKADGTYLTKYDWSGNVRDMDFHGVYGDEVGSWYLNPGKDYFNGDQLKQELMVHRESQTGDAVQLNMIHGTHYQAISRDAFADGKVWGPWLWYLNDGSKDDAAKRAQEEEEAWPYKWFDDKEYQSRGKMQGKLVLSDGRPAAGAAVFLGDNNNSTISTLDQGKGYYYTTYADEDGKFDIHDIRTGTYALYAWGNGDSIADVTTNFTQNDVVIKPGKNTNLKELTWKVTDTKNRIFQIGAFDRKTDGFALSGPTPFEHARISKCPANLTYTMGKSQTSDWCFGQALNGTWNINFPVKTAPSDSKLTVSLAGFSQGTSATILLNGVQVGNITSASLVNSQDTYRGATRNGEWRLLEFAVAKEGLKMGENRIDVGVEKATQWRGWLWDSIVLERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG99</accession>
    <name>A0A9W8YG99_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG99"/>
    <dbReference type="OrthoDB" id="3794996at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="133" mass="15257" checksum="2BE052DD3FF9748A" modified="2023-11-08" version="1">MSHTATKMPTVYDFFTLSEHDKQRIMALANHVALDWQIVSPASELPYKYHPDPDPHNWGVDVLAALHRLAKMTQVKLSDKQQAVQLVCDSIQNRNRGLDVIHGDYVIRPEDINFAIEKIENPVDSYRRGYADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGD6</accession>
    <name>A0A9W8YGD6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_000432</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGD6"/>
    <dbReference type="OrthoDB" id="3257095at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF14">
        <property type="entry name" value="GABA PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="369"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="395"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="439"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4377605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="509" mass="54845" checksum="EBE105A3850CF5D0" modified="2023-11-08" version="1">MADDAKLKRHLPTDGDDERLAQLGHVQELRRDYSIWSLISLCLCLMATWEALSTVIATALTSGGAPCLFFNLLMSIICTIAIALSLGEIASIYPTAGGQYHWVAALSPPSTRSVAAWSTGWINVGGQIVLTASAAFAAGLQIQGMIVLNNDTYIPARWQGLLLYWAVLVYAAVMNIWGSRLLPTANLLAGILHVAGFFAVLIVLGVMAPKNTASFVFTEVSNSSGWSSDGVSWLVGLLSAVYPVLGYDAACHLAEEMPHASRNVPIAMVGSTVVNGILGLGFCIMLLYSTGPLETLLETSTGFPFMQIFLDATKSATGATVMSVIVNVIAIAATVAGITSTSRTLWAFARDKATPFDRYFSYVNHKSQIPVRAVVVVTILQMLLGFIYLGNSTAFNAVLSMAVLGLYASYLIPIIYFMVYGRPNFTAHDFGVFRLPKGLGLSLNLLACAWLILAMVFSTFPTLMPVSPQNMNYSTVVIVGWVVLGGAYYFINGKHKFEVPVIDREARGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGK6</accession>
    <name>A0A9W8YGK6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGK6"/>
    <dbReference type="OrthoDB" id="3219396at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="5"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="412" mass="47404" checksum="385FFAE33C3F1E45" modified="2023-11-08" version="1">MSRPATSIASLPTELLFNVAAHLTHLDRNQDLSNLARVSRQWRPIAQEQLLKHPRFSLTHIAEYMWELGLRPHLAPHIESLEIWSKSEGRVRRDARGTSIKEYVPTPAPLRMTLRQSEMMDKCAAFIKHFARTRRESRWWRDALLEDVVPALFGVLLCILPNLRALRLGKAWLKDFPIFSYLLARDSSTVQFVVPSAWKGDCFAGALTHLLPRLEVLDVPADMVAMYFHSRTTAIFDFRDFGNLKELGITFKALSGFGITRHVSLNPRNMFPKSLEVLKISEASENSTLFLGNLCQVKKEGHFPALRRVEVYHAYGIAWTRNAAQAITYPDPVDDVRLLFRDAKLELYLYFPPCILRTWEVGGTPWMLKTESKALKRAERVCFKHVTNFYDVEHRFPRLEAEWDVDGDAVMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YI11</accession>
    <name>A0A9W8YI11_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">O-glycoside alpha-1,2-mannosyltransferase 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">omh4</name>
        <name evidence="7" type="ORF">N0V83_001514</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YI11"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF2">
        <property type="entry name" value="MANNOSYLTRANSFERASE KTR5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="311"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4376231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="513" mass="59568" checksum="5EBC8A38FAE76785" modified="2023-11-08" version="1">MEGSQDVTRRVPVSKEKLDKPDSSSHRAEKPSQRLSFFHQRIRLKGDSSVSIPLALVLLFPCLVMFLILVLFMRSPDSEGMMNMPTGTSPSTRKISEKYDKPFAIGCLEPQVNGPRAKAAFVVLARNKELEGVIQSVKSIERHFNRWFHYPYVFLNDGDFNETFKETVLNYTSAPVEWGKIPKEHWGFPDWVDPAVAKEGIAKQGDHAIMYGGMESYHHMCRFYSGKFYQHELLQKYEWYWRLEPDIKYFCDITYDPFIHMIRNKKTYGFTIAVKELRETVPNIFRYASAYKRMHNLTSKGLWEMFVEKPKEQDEKPLPTTDPRYKKPLSQDIPDPESSVRDLPEIDPEAMEGESYNMCHFWSNFEIARLDWFRSKEYNDFFELMDHSGGFWMERWGDAPIHSLAAGALLGPEDIHYFRDFGYRHTTIQHCPANAPARQLPRTRYLEKTTLDEKKRLEEDAYWATPDPVKENGVGCRCKCDTDIVDVEGKEGSCLAEWVDVAGGWASPGPQWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH42</accession>
    <name>A0A9W9CH42_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AA9 family lytic polysaccharide monooxygenase</fullName>
            <ecNumber evidence="5">1.14.99.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Endo-beta-1,4-glucanase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Glycosyl hydrolase 61 family protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_009665</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Lytic polysaccharide monooxygenase (LMPO) that depolymerizes crystalline and amorphous polysaccharides via the oxidation of scissile alpha- or beta-(1-4)-glycosidic bonds, yielding C1 and/or C4 oxidation products. Catalysis by LPMOs requires the reduction of the active-site copper from Cu(II) to Cu(I) by a reducing agent and H(2)O(2) or O(2) as a cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[(1-&gt;4)-beta-D-glucosyl]n+m + reduced acceptor + O2 = 4-dehydro-beta-D-glucosyl-[(1-&gt;4)-beta-D-glucosyl]n-1 + [(1-&gt;4)-beta-D-glucosyl]m + acceptor + H2O.</text>
            <dbReference type="EC" id="1.14.99.56"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 5">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="5">Has a modular structure: an endo-beta-1,4-glucanase catalytic module at the N-terminus, a linker rich in serines and threonines, and a C-terminal carbohydrate-binding module (CBM).</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.56" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH42"/>
    <dbReference type="OrthoDB" id="5558646at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030248">
        <property type="term" value="F:cellulose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21175">
        <property type="entry name" value="LPMO_AA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049892">
        <property type="entry name" value="AA9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005103">
        <property type="entry name" value="AA9_LPMO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33353:SF13">
        <property type="entry name" value="ENDOGLUCANASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33353">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12560)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03443">
        <property type="entry name" value="AA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040812089" description="AA9 family lytic polysaccharide monooxygenase" evidence="6">
        <location>
            <begin position="18"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Auxiliary Activity family 9 catalytic" evidence="7">
        <location>
            <begin position="18"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU368122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4363372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="222" mass="23484" checksum="8D5B1B50166F53F0" modified="2023-11-08" version="1">MKFQLLSTAALASTISAHTIFQKVSVNGADQGQLKGVRAPDSDYPITSVTDSAFACNKDLKHQDSTVITVPAGAKVGAWWGHVIGGAQGANDADNPIAKSHKGPISYYLAKVDNAATTGTSGLQWFKVAHDGLSNGKWAVDTMIANGGWHYFTMPTCVAPGDYLMRVELIALHGAQNQGGAQFYMECAQIRVTGSGTNKGSGFVSFPWRVQGKRSGCAGQYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH74</accession>
    <name>A0A9W9CH74_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH74"/>
    <dbReference type="OrthoDB" id="4359806at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040982875" evidence="1">
        <location>
            <begin position="19"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="228" mass="25310" checksum="0D10604DF7BD06D2" modified="2023-11-08" version="1">MRFLNVLPTAFLAATAYAAEDTSTPSSSSSSSAATPAVEEPTPIGALWTANWTTSTLSPYTQHCHNTNTYHAKIYKLNELYPDLKDQAPQLKVFYNKQYYAGSWGGIDVHGTGRELIKMEMAELPYKVREWLKREPKQRHFSVQDDAVFFAPGAIYPILPLWVDDAEEGLECEGVFDDLENYSNEPKDGAVIGKVMHTNTGEKQVKFTVEALQVKAKKTGAAAAKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI80</accession>
    <name>A0A9W9CI80_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">dihydroxy-acid dehydratase</fullName>
            <ecNumber evidence="14">4.2.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ILV3</name>
        <name evidence="19" type="ORF">N0V83_009880</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:24809"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24810"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:27694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:27695"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IlvD/Edd family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.9" evidence="14"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI80"/>
    <dbReference type="OrthoDB" id="3851628at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004160">
        <property type="term" value="F:dihydroxy-acid dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009082">
        <property type="term" value="P:branched-chain amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.80:FF:000001">
        <property type="entry name" value="Dihydroxy-acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="entry name" value="IlvD/EDD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00012">
        <property type="entry name" value="IlvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050165">
        <property type="entry name" value="DHAD_IlvD/Edd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004404">
        <property type="entry name" value="DihydroxyA_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056740">
        <property type="entry name" value="ILV_EDD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="ILV_EDD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00110">
        <property type="entry name" value="ilvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002068">
        <property type="entry name" value="PRK00911.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21000">
        <property type="entry name" value="DIHYDROXY-ACID DEHYDRATASE DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21000:SF5">
        <property type="entry name" value="DIHYDROXY-ACID DEHYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24877">
        <property type="entry name" value="ILV_EDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="IlvD/EDD N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00886">
        <property type="entry name" value="ILVD_EDD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00887">
        <property type="entry name" value="ILVD_EDD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 19" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroxy-acid/6-phosphogluconate dehydratase N-terminal" evidence="17">
        <location>
            <begin position="73"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Dihydroxy-acid/6-phosphogluconate dehydratase C-terminal" evidence="18">
        <location>
            <begin position="405"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF24877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ4363584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="601" mass="64151" checksum="E8A37C397458DB95" modified="2023-11-08" version="1">MSLARFGGALWRPKCSARPQWHAARALSSSAIRTARADNELNSVSKNITQPKSQGASQAMLYATGMSEEDMNKAQVGISSVWYTGNPCNMHLMDLNHKVREGVERAGLLGMQFNTIGVSDGISMGTKGMRYSLQSREIIADSIETVMGGQWYDANISIPGCDKNMPGVIMAMGRVNRPSLMVYGGTIQPGCAKTLDNQQIDIVSAFQAYGQFITGEINEDQRFDIIRHACNGQGACGGMYTANTMATAIETMGMSLPGSSSNPANSQAKMLECLAAGGAIKNLLKEDIRPSDILTRQAFENAMVVISITGGSTNAVLHLIAIADSVGLKLTIEDFQSVSDRIPLLADLKPSGKYVMADIHDIGGTPALLKFLLKEGLLDGTQLTVTGKTLAQNLENVRDFPAEQKIIRPLSDPLKSTGHLQILRGSLAPGGCVGKITGKEGTVFTGKAKCYDAEDAFISALERGDIKKGEKTVVVIRYEGPKGGPGMPEMLKPSSAIMGAGLGKDVALITDGRFSGGSHGFLIGHIVPEAQEGGPIGLVRDGDEITIDSEANELNIGVGEKELEERRKQWKAPPLKYSKGTLFKYARFVKDASQGCVTDSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIP3</accession>
    <name>A0A9W9CIP3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIP3"/>
    <dbReference type="OrthoDB" id="3751150at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="649"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="649"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="680" mass="75939" checksum="97197FD5D535D3EE" modified="2023-11-08" version="1">MGTTCRTPSPRRESYSNCDDDSSPEDQQPSSPQLVPQASQNFSGCQKSESTRLLGHWHGLPSPQTPTFTHDRYGTLPMVNTLQPIPTQNGEDDATTATISKTKVQFGAEILQQVTERDTDLARELEAIPSPTSLRRSSRISTKTKKPVLDVAQPAVAPVPKSAKTGPMVPTAPPQEISIPPFQIDPIRFEGLPALHTVDWQLQYPPGGSAHPSYPYPSLDPRLIFHENMVPMSNGLPDISRLPKLLLPMGWSHISWSGLLPIVFDPYRQAFKLTPVGPMSITCEELHQNGLYKYVPGGEQHPETALLPQMIALSDGSDAEVYDWEGVDWVLPWNEMNNASGSQSTSVGLASPVRTEFDALQHAQQLIAPAEFIHYNEGWDCPDGIVDLTDAWRWLGEKEQKPGPIFKPSKGKTWKGTGIHLVSRRFKQPIASLMAGAIATITDDPEGYLVKQDQREFCPFRSVATPINVNIALLADIEITLKELLCYFPNHFQWRKCGDRLVRAGLGGSDIANMINMTRCLSGDAARKIGTISHHITYETGEDGTRVRIVRSDDEEKKYTADGWTYEAWELSNYPLLGLAHGLLELPQGEDAGPLTRIIRWCRTQHRYNTLLSEVSTLLDEEGVSKQIERTFEDCPDKGMLARHAEAMKEDRKRVLKSEKMKRLALEDADNDQGKRRRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIV7</accession>
    <name>A0A9W9CIV7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRAL/TRIO N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008522</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIV7"/>
    <dbReference type="OrthoDB" id="30289at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL/TRIO N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="89" mass="10240" checksum="93CD0E7C6C433897" modified="2023-11-08" version="1">MLRFLRARRFIAPEAFKQFKDTEDWRKENHLSEIFNTIETEEFEQTRRLVHGPAYSAARLCLTAARSIPSGSVDATSAAFPSFFSKSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ00</accession>
    <name>A0A9W9CJ00_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosome biogenesis protein SLX9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_007829</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLX9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ00"/>
    <dbReference type="OrthoDB" id="5429132at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028160">
        <property type="entry name" value="Slx9-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15341">
        <property type="entry name" value="SLX9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4366194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="172" mass="18969" checksum="D6FEC9D6766CBE65" modified="2023-11-08" version="1">MAPIKKRTTARAKSARAARPATSTATLPTPEDASSLFPSSKKDKQKIKHSSFVSKIEKSSSKTTKRRRPNKKLVATLESLADALPGLEDEGADGEIEVGQAKIQRKSLKSMRSRPGALKRKEKLEKLERDRFNKNLAQLASSTQGSAVQDRWAALKSHVQSTMEVKPEFAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ55</accession>
    <name>A0A9W9CJ55_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Trafficking protein particle complex subunit BET3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_007995</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Golgi apparatus</location>
            <location evidence="1 8">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ55"/>
    <dbReference type="OrthoDB" id="10262857at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14942">
        <property type="entry name" value="TRAPPC3_bet3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1380.20:FF:000001">
        <property type="entry name" value="Trafficking protein particle complex subunit BET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016721">
        <property type="entry name" value="Bet3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13048">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018293">
        <property type="entry name" value="TRAPP_I_complex_Bet3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR018293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4366359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="194" mass="22098" checksum="04A4523340B103D4" modified="2023-11-08" version="1">MASSTKAARLGEEVWKRRTRVDKVSAELVTLTYGTIVAQLCKDYEYNYADVNKQLERMGYNIGVRLIEDFLAKSSAPACTNFREVAEMISKVGFKIFLNVTPSITNWSSDNKQFSLIFDENPLADFVELPDDGRAQDELWFSNILVGVLRGALEMVHMQVEAHFISDVLRGNDTTEMRISLIRYIDDEMPPDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJT3</accession>
    <name>A0A9W9CJT3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJT3"/>
    <dbReference type="OrthoDB" id="5389734at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="465"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="513"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="553"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="583"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="615" mass="68744" checksum="4088EE6DC8CA7511" modified="2023-11-08" version="1">MVPFRRLSQKARRSFENHRDPLEWHNERDKHIMTATFPIHPLPSMQAPFEESMLDATGETGHVPFVNPKKGVKTRQQLLCRGEGASELSWNFTYNCHWRNNPKGKFHPLVKTVTQIVFGLHLLHQHLEKSVADVADILLKHVNELDSFLQRANEDLESSLKDMLFRHKCLKVPMEHVNEFDRLLEDRAYRAQLLDGNVVIERTIGRMSGMLNDYLIDINTFQDANQKLDLYLLEIGDAWTYDNEDVGRIYSAMCGNTGGWSQFLQSLVAKAEKLGLVLVQVSSYCNEIEKRCGAASRRSMVRMPGKWKFFCNPADRLQIATRTSSRNSSNSRDNGRGFRNITNNKPLPTPPSERPPFMPSPNNGSEPASDVETPQRYSQMPKPRPQAQPDLVNTSDSAAETPKRSEDSASTPATSDTDVDVRRQSAPKQPEYYRESWHNDDQRGRAAPQADSGRTNRLFGHRRNPSSVSRGSTEETLSTPQRLSVKPDSPPLTGKDSAYSSVSGASGMSPTVASPRSASSMSSRQTAQFGLFPTRNLTPKGSISGRLGAMSPAFSQTSRSTKPAETPTASRPNTSFSNYSDASSTKRLSKRSSFSSLKRLFTKKKSSGEIDTIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK56</accession>
    <name>A0A9W9CK56_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">4-aminobutyrate aminotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_007374</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK56"/>
    <dbReference type="OrthoDB" id="5419315at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000013">
        <property type="entry name" value="4-aminobutyrate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF79">
        <property type="entry name" value="ACETYLORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4366846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="450" mass="47987" checksum="904F59416FC34470" modified="2023-11-08" version="1">MSSDLDTAAFAEQHIAKGIGRLTKHVFDEGRGTFITTDKGVKLLDLTAGIGVVNLGHCHPKVSAAAAAQCSKITHAQVNIGFSSAQIALLKELLPILPDKSLDTVFLWNSGAEAVEAAVKLARAATKKPNIIVMQGSYHGRTNATASMTRSKTIYGEGHGPLMGGVFATAFPYYSQFGAPSDTPTEELVNQALLQLKLTLQQSTSPADTAAIILEPVLGEGGYVPAPPEFLRGLRKVCDDNNILLIVDEVQSGMGRTGHMWFIEESGVRPDVLVFAKGIANGFPLSGIASSKKLMDLQKPGSMGGTYAGNAVACAAAKATIEAFREEKILDNVAARSKQLFSFLRDLKSSNTKAGNLIEDIRGSGLMVGVQFANPEVQRDSSNTASRNAQSQPQLAPKIVQECVKRDMLLLSTSVFDVLRFIPPLTITEEELSQACGIFKESLEAVAKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKI3</accession>
    <name>A0A9W9CKI3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_006870</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKI3"/>
    <dbReference type="OrthoDB" id="432010at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032465">
        <property type="entry name" value="ACMSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240">
        <property type="entry name" value="2-AMINO-3-CARBOXYLMUCONATE-6-SEMIALDEHYDE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240:SF30">
        <property type="entry name" value="AMIDOHYDROLASE-RELATED DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="4">
        <location>
            <begin position="77"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU366045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4367289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="348" mass="38018" checksum="9A1FEE1956061D80" modified="2023-11-08" version="1">MATTPSNLIATVESYLSPTLAIQPSTSNDPSAPAFHLLPLTTQAKLRNFGPIRVKDMRALGHSKQIISHIPFPASALTCAKLNDALYAANRLNADKFVPLALLPSGAGEGKDAARELQKCITKYRFVGGVLGLGPGSSRSGNLDDRSFDELWALAEKYRVPVALREMWPIESELSKTYQHNLPASVLAPLVSHIHAVLNSQTALYLLHLYLSSTFDRFPSLRLILCHPGVLPSQFRRITAFLALIPDADKPKRPFLDVWQHNVYLTTADVQDMSSLRSLLELIPVDRVLYASNYPFEERGKELMEELRGSGFLTGRSGRGLLGECGGTVWIEGGDVGKAAGGVPGYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKK2</accession>
    <name>A0A9W9CKK2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006757</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKK2"/>
    <dbReference type="OrthoDB" id="2150604at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="88"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="217" mass="24086" checksum="2AD2CDC7F513666A" modified="2023-11-08" version="1">MTYDDDVENREAASERPPTSGGASHQPLTHDQDQHKWSVLRVLQFFGGGIYAPDPSTYDPIEILLNTEDTEQRDELTVRWRDNKLSELSFIGVVAALLAGVLTSTGSWPNILPNGKQSPWPVRTSWYCGIVLALFSILSAANQTVRLHRLPSKAKLASNHGLADARDVLDDSDNLYDCGPELWDDNTKVAITYTVIALVAIIMFFMGQIAMHAPIRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL11</accession>
    <name>A0A9W9CL11_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_007176</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL11"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000386">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000065">
        <property type="entry name" value="Putative MFS pantothenate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF37">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="377"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="435"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="469"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="75"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4367591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="529" mass="59611" checksum="092F2098C3677DDD" modified="2023-11-08" version="1">MASKDEVEIQSKGLPEASSSRETSPPAPNVLKPWEKRGFVGSTRRTFRTIQRYVWDDPDKSPEEKWFLRKLDIFLLVAACLGYFSKNLDQANINNAYVSGMKESLNMNGSELTYAGNCFTAGYVVGQMPAVMLATRLRPSILIPTLEVIWSVLTFCTSSVTTVSHLYALRFLVGLCEGGYFPVMIYLIGSWYTKQERGKRTALFYCTAALASMFSGYLQAGAYTGLDGVHGKQGWQWLYIVCGIISLPIAFLGYFFIPDFPETTRAFYITKEEAQRQCQRLIEEGQKPLGHNPWNRKKFFNIVKQWQFWVLPLGYFFVQASYPSHQPVMALWLKSKGHSVYERNVWPTGQVAIGVVVQLLAGALSDSPLLKGRRWPAIVVMQATTVLGCIILAVWHVSDNAKYFAYYISYFSAGVPGPYYAWYADLIPHDHEMRGFVIAASNMFSYTMVVWYTIAVWRTADGPRFKPGFIAASVFGAALILLTQVLRLLQRRDSKKRARETEAVRDVEAPVASISDPKFAADAVRTKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL20</accession>
    <name>A0A9W9CL20_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_006786</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL20"/>
    <dbReference type="OrthoDB" id="5428055at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="502"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4368429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="543" mass="61406" checksum="DCB8D623FF21E092" modified="2023-11-08" version="1">MGPSDSRSLHTYLAKSSGSSYHFLSDEELEYMSLIQTRAQPYFPRFAAALAEQTPFEREQGRCAAVDFTNPQDHQITSFATATELHQYLGNDVSPPPYHTVPELPTRRLFILEDLPCNHILALGSRLRIPPSFFAGHWEDPASSTFNHRNPFQRCTLPNFRLRYATANRVEVDAPTPGHGGTSPNVFTFDTRVCRYLHTYNRGGLVYDEARSHHAMSFWSSPAREDGSWHAVLLVDPPLGQHVRCMTTKQLIPLRTRLADETSMPKYFLNPEMQTMDELPQSCSQWSSSYSSPGYISMFDDTLQSFSSNTSAPITATHNPLSVVAFPRRLVISTLIAYIRRRYLNLVSIQNSSFKPHTMRHNYLASFSKSNYSSWNSDLFDFIVGSRAAIRVFAGEMDDNATALALHNTPSDCGAAAAVVEQWERDGWQSIRELTAVVEETVNAFATGYLQYVTIQEAHVSNGNAQSLARITVLTMLFIPLSTVASIFSMGGDFLPGENRAWVFWVVAIPLLAVLAWLYWYRQIMRAWRERKEAVLPLFREKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLH6</accession>
    <name>A0A9W9CLH6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_006699</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLH6"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000064">
        <property type="entry name" value="MFS allantoate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF97">
        <property type="entry name" value="ALLANTOATE TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G14700)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="56"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4368343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="426" mass="47552" checksum="EBA1E3A5022CD416" modified="2023-11-08" version="1">MAESKEQYMTSDAEKHTDASHDLRGDIALGVFEEIREGALGSVDDSKLLWKVDMRLMPLLCITYMLQSIDKTTLGYAAVFDLREDTQLHGTQYSWLGSLFYLGYLFWEYPTSLLLQRLPVAKFMSGTVIVWGIVLMCHAAAHDFGGLAAARTFLGMLEASINPGTMLIFSMWYKRSEQPLRMGIWIGSAGIGYVLAGIASFGIGHIGGALSSWKYMFLIWGAVTASWGVVIYLVLPDNPVKAGFLSENERRGVIHRIRENETGVENKHFKKTQFIEALTDLKTWLLFIFAVASNAPNGGLTTFQGLIIKGMGFSTLRTTLIQMPSGGVQMKITTNAFLLIGYCLGNFIGPFFFLAEQAPQYELGVGMMFFCIGVQVLSIVGLWVLLWMRNKSRASANVSEQDVIEGYENGFRDLTDKENVHFKYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLP5</accession>
    <name>A0A9W9CLP5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_005476</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLP5"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000078">
        <property type="entry name" value="MFS maltose transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF10">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="369"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="405"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="440"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="18"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4369713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="525" mass="57906" checksum="9DA8CF5DC89690EE" modified="2023-11-08" version="1">MATIKGESWRSHWRLFLACGIVMLSPFQYGFDFGMIGGLQAMKGFLQIFGYQTTKTVTGWNITTERQQLISSLMTLGAFISSATAGVPSSRVGRRICLAAACITCFVANIIMMTTESIGALYAGRLIIGLANGYFMTFSQLYLQECSTAELRGVFLTGFNFVVSFGTLIGTIVDWATAKRPDKSAYLIPLGLIYVFPFIIVCTIWFIPESPRWLILQNRYEDGVKALEWLRPTGHDSRAEALAIQVAIDKEKESSSGVGWLDMFSNPIDRRRTTLAVCAVLLQAASGSMFIIAYKAYFLQMAKVSDPFAMSNVLSAISICAFIANSFIVVRYGNRRVLLITGLLVCGILQLIIAVVYHKQPGAHSTGKVTVGLTAIYMFSYNGMISTYAWLAGGELPTQRLRSHTFGLAASVGFVGAWLTTFTAPYFINPAAMNWGPRYGFIWFPSCVIGATWVFFFLPEAKNRTLEEIDEMFEAGLGARKFGSYKCVGVAASLDPESRKSIEHEKEISVLRDEKVTAGNTVTRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLS3</accession>
    <name>A0A9W9CLS3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">argininosuccinate lyase</fullName>
            <ecNumber evidence="4">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ARG4</name>
        <name evidence="8" type="ORF">N0V83_005964</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLS3"/>
    <dbReference type="OrthoDB" id="2561043at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.10:FF:000002">
        <property type="entry name" value="Argininosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000001">
        <property type="entry name" value="Argininosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000025">
        <property type="entry name" value="Argininosuccinate lyase chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814:SF1">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="6">
        <location>
            <begin position="15"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Argininosuccinate lyase C-terminal" evidence="7">
        <location>
            <begin position="373"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="465" mass="51817" checksum="57FAC1BECB96FF72" modified="2023-11-08" version="1">MASEKKVAENMLWGGRFTQGLDPVMEQYNASLPYDRLFYAEDIAGSIAFARANKNNGILTADEFAAIEKGFAQIKEEWASNSFEVKANDEDIHTANERRLSEIIGKDTGGKLHTGRSRNEQVATDMRLWLREQLRTLEGYLKLLIQTSVQRAEAEIDVLMPGYTHLQKAQPVRWSHWILSHATAFASELERLREVIKRVNRSPLGCGALAGNPFNIDRDAMAKELGFEGLLTNSLNAVGDRDFVFETLQWGSSFMLKMSRWAEDLIIYSSLEFGFVRLADAYSTGSSLMPQKKNADSLELIRGKSGRAFGHMAGLYVTIKGLPTTYNKDLQESVEPLIDHIKTVSDSIQIATGVLSTLNINAEKMHAALAPEMLATEFADYLVRKGVPFREGHHISGRVVALAENENMPMDKLSLEQLKGIDSRLGDDVVECLDYERAVELKNATGGTSKSAVLEQVQILKKILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLT8</accession>
    <name>A0A9W9CLT8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Beta-glucan synthesis-associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">KRE6_1</name>
        <name evidence="12" type="ORF">N0V83_006174</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SKN1/KRE6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLT8"/>
    <dbReference type="OrthoDB" id="412647at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015926">
        <property type="term" value="F:glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02180">
        <property type="entry name" value="GH16_fungal_KRE6_glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005629">
        <property type="entry name" value="Skn1/Kre6/Sbg1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31361">
        <property type="entry name" value="BETA-GLUCAN SYNTHESIS-ASSOCIATED PROTEIN KRE6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31361:SF1">
        <property type="entry name" value="BETA-GLUCAN SYNTHESIS-ASSOCIATED PROTEIN KRE6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03935">
        <property type="entry name" value="SKN1_KRE6_Sbg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="193"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="11">
        <location>
            <begin position="267"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="100"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4369091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="664" mass="73407" checksum="0C82C442B6FFF418" modified="2023-11-08" version="1">MSSPLSPRGNGPPHIRLNSGTQEARTDDNSPPAMRPGTARTNSARSLTQPVRPGTAPLSALRSPTVPAVYTNAGVAESAEMLLPPRKLKTKQYRDESPIRSPSAMSSRRTSWSSDTTRDSRGPFASPFDDSRAPSRAGSEEDLNTQTVSEKFNILPSAGLLLFPEDVEKDDYLHNPDPNDKDKNECNIWNRRGLANVGGLGFLAIGILVLFIGYPVLTFVDKLTETERTPCTDNPLCIKGKENEPLLSNLRTGLIDPDTPASAMSRTSYDGTKQVLVFSDEFNTDGRTFYDGDDPFLQAVDIWYGATQDLEWYDPDAVSTANGTLNLKFDLFKNHGLNYRSGMVQSWNKLCYKGGHLEASISLPGRGDVSGFWPGFWTMGNLARPGYLGSTEGLWPYSYHDECDMGITPNQSSADGLSYLPGMRLPACTCKGEDHPSPGKSRSAPEIDALEGSVHFMGPGETNAVGVVSQSFQAAPFDIWYMPDYDYLEVYDTQITSMNAYKGGPFQQAISGLTNLNNDWYNGKQYQTFSFEYKTGSDGFITWYVGEDKTWTFKAEATRPNGNVGTRVIPEEPMAIIANFGMSNSFAAINLADIQKDLPATMRIDYIRIYQPEDNQIVTCDPDGYPTTDYIENHPEPYANPNLTNWKSTGFEWPKNSFMDDCKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM81</accession>
    <name>A0A9W9CM81_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS7m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_004466</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM81"/>
    <dbReference type="OrthoDB" id="9972728at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14868">
        <property type="entry name" value="uS7_Mitochondria_Fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.455.10:FF:000006">
        <property type="entry name" value="37S ribosomal protein S7, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047988">
        <property type="entry name" value="Ribosomal_uS7m_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="9">
        <location>
            <begin position="170"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="40"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="102"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="118"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4371249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="337" mass="36723" checksum="840DF33E9E120A0D" modified="2023-11-08" version="1">MPPRLNITTFTRSIAFRPKPQVQWPARSAARFSPSQCRLYSDANDRSKRVDAKPLEHVSEEKAAVAEAMGEQGPDMNQGTPVEEVVKGDKAAQEKLPKVMQDKIKAQSSNSAPKGSRSYSTMTTQSSGSQGLDMGLVDLQSATTAPATPGLKFAMPTLPLPKDGHVKHRYDPVVEQVTNLLMRHGQKSVAQRNMALILQHLRTSPIPTINPQRPLLPGAPPPSHLPLNPILYLTLAIDSVAPLMRIRSQRGAAGGGVALQIPVPLGQRQRRRAAIQWIMSAASKRRNMGSGKGSFAQRIAQELVAVVQGTSSIWERRNAIHKLGVAARANIVLPRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM88</accession>
    <name>A0A9W9CM88_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_005600</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM88"/>
    <dbReference type="OrthoDB" id="5368516at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040874241" evidence="3">
        <location>
            <begin position="21"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="308"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="418"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4369836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="449" mass="48791" checksum="BF1099FA9C37DB22" modified="2023-11-08" version="1">MTYFLLVAALIYILVIPVEVQSLRRDFTNVVSTSRVAEVSLFSSGMIITFFHLFLRTNAMRMVIRPIEEMKGTTKQQRPKIRFFGPSDLEMNISGPMALQSSRLPESRQGLIDVGPEKNRFDFDPEYFVRPEKAVTPASVKPESPIDPTKWPLPPDVEENKVATPGHERGKKSYSLFPTRAEDVPRLPATVYDPTAAKDTRVSKLAMRRFTRRSSITNVSEAFDFLTKPKPLFAAADRHRRDRSTDSSATVQIGLRFSVAPATLAAAKCTQTERNVEADSIAMPPPPPLKRGETQSSGESLALPIQHPSTTNSSSSDELPSPSAFPQPPARESSPTRPRMASPARTPAFPLIPAQHSSAYLKAQREKVLPPPPPRSPLSTPKIATQPSASTPAAVMSGLHMNPVTPVTSVGSPTASLPGTPARAAPSPTARIPLGAGTMSRSPPPNGWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNF6</accession>
    <name>A0A9W9CNF6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADP-dependent mannitol dehydrogenase</fullName>
            <ecNumber evidence="4">1.1.1.138</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_003189</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.138" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNF6"/>
    <dbReference type="OrthoDB" id="1888931at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050085">
        <property type="term" value="F:mannitol 2-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019594">
        <property type="term" value="P:mannitol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000090">
        <property type="entry name" value="NADP-dependent mannitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF1">
        <property type="entry name" value="NADP-DEPENDENT MANNITOL DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00066645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00069279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4372898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="276" mass="29432" checksum="0DAA52B1A4EE88B6" modified="2023-11-08" version="1">MSMSIEECQRPRPGFPRPVPDTPLNVLEQFSMKGKVIVVTGAAEGIGGAVADAMAEAGGNVALWYNSNPAAEQRGTTLSHSHGVKTKAYKVDVTSAEAVENTMKQVVDDFGRIDVFVANAGTGDSKPLLEQSLDSYHNLMKVNVDGPVFCAKYAGAVFKQQGFGNLILTSSISAHIVNVPVDQPIYNGTKAFVSHLGKSLAREWREFARVNIVSPGFFDTKMGAGPQVVTEGLRMTTLGRQGDVKEIKGLFLYLASSASSYVTGSDVIIDGGYVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNG8</accession>
    <name>A0A9W9CNG8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNG8"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4372943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="245" mass="28223" checksum="6AE7032C98A7512A" modified="2023-11-08" version="1">MKADDEVVGPTYNPARNTAESPYQSIDNLKEWFWAPFPKYLINPVIHDFYNAWGVGYALVDLGINPISHEYEGGKNELFFLDHQGFDPAFPDVDKQWYQINGKEYRATGASYAFTINSEDGVIMSLNRKSPRYAAKERNPPVPDDELPKLNQFSDVAWIGWDTVSQREGVDIKNLRYFLSIGIDNTDTKAIIIRAMNSRGWQLSEWPGHIFEMEWMETQAILGKSKMPACADYTQLLNEWRRRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNN8</accession>
    <name>A0A9W9CNN8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003978</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNN8"/>
    <dbReference type="OrthoDB" id="2537141at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="463"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="394"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="669" mass="74762" checksum="CB95CD01D789A292" modified="2023-11-08" version="1">MDIRRWLEETVQPEHLSSLPGNTIAASFCHKKPGPTTSKRHRREQSKSDSSLLDPPLREKPPTRKQKRTAEASTDHSAGSEASHPTHSETTESDVSSHRYARRPRRKTHPERYEPSSKAVKERGKHAYRSRKGESNKSQRKSKRKKCEKACSGTVQCFHAKNVSGDRLTLKPREQLGLFNKGKTSMAVKGRGLPDLVFSEMKFLQQQKDQPELIPEAEPPKKKRKSNYTHTKEDEISAFFTSVRPVLAEKDGNIQAKGTSRTHDTSLELRHRERGRSSLAVEDATPTVPTIEVSDKASYLGFGSRGPRHESTSVVSWSDSTREPSTKPALARTKHVLYFRRLDSINPGEHGKTTDKQDVSFKRPAPLSSGPRRTNGTSERFEVSSLAPSRQRVSRSQSHPRYTSSPRRVNLVDRAAKFRSTDTIDSPSSMPPVAPVHAAAIDARQVQVADEYNASKFRAASIASPGASSTYRQPTLDGEDTDAGFNPQTSSDLGRVIQKCNSTFHERRRAATPQRRHPMPEILSRFADEAESQSSRGFMLYPANHRTPIVRFAGVENRAPPLTSLAGPSIYEQQAQRQQLPLQPMAEENFLQDTDIAQECFDETEDMGCGTQNWEESPEDGSSWSPFEVPWSFAGACPMQTVGSDVRHGGAPRPLLPAGQPHYEIFRQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP31</accession>
    <name>A0A9W9CP31_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1989 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002719</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP31"/>
    <dbReference type="OrthoDB" id="504708at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018959">
        <property type="entry name" value="DUF1989"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31527">
        <property type="entry name" value="RE64534P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31527:SF0">
        <property type="entry name" value="RE64534P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09347">
        <property type="entry name" value="DUF1989"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1989" evidence="2">
        <location>
            <begin position="338"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="531"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="551"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="611"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="621"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="662" mass="70885" checksum="6A1223CA15BF1852" modified="2023-11-08" version="1">MQNNVKTAPSIPSRRALAGLALVSVLSAVEKQRKKKKAQDGGGEDFAIQDMSGELQTIPARHGVATFVPRGRTIKVINTYGKQVVSMWAFSLGAPPEDEEEGEEIEEKEVDEEQVQKEAEGLKKAVEEGGGMEAESTDKGKEKEPESPGKGKEKAHDAEGTGKETQTTSEDEADDPPEQAPDTPDNEKTTETTETTAASSSKQPSKRTWASYLPSIPYRNKGTAGKQDEQKKPSAEEEKAQNEENTKKWSSYLPTGKGFSSYVPNVQIPDSKGVVSAFKSSHYRDPNKSYAEQLYDFSKTPVGAGTIAAATGSGYASSVYAAYSAYAATHPSSSALPPMEYLSLPHTRAASHKLVPEIDDELLTNLRNPIITLIEDTSPGAHDTLTAACDANLYTALGVDKPAEHGSCAENLVLALKELNENAGLKGAKAVGADISVNIAPTPLHLFMNAPIELANNASSEGAGAKGAILKVEEAKGKKRSFVRFRAERDVVLVFSACPMDVGKQNGGRCMAANFMVEDVQEGEDLATSAASIGKKRGTPKKLNGSAAGAKKAEPTQKADVKKEEPKKPETKKAEVKEESKKEEPKKEEPKKEEPKKEGAKKEEGQKKDAAAPSKAPAQKKTSEKAEEKTKKEESTSEEPKAKKKPKKLQVRGSGAGTPAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP46</accession>
    <name>A0A9W9CP46_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004295</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP46"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="76" mass="7379" checksum="C09BC9DE7DF6A155" modified="2023-11-08" version="1">MRSSPYLGNSSRISTMSSTSAPTGSATNKIPVTGFAGATATSSPGTGAAPASFMPSAGLSVAAVFGSIFFGFAVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP78</accession>
    <name>A0A9W9CP78_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD40 repeat-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_003007</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP78"/>
    <dbReference type="OrthoDB" id="4869960at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045717">
        <property type="term" value="P:negative regulation of fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045151">
        <property type="entry name" value="DCAF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15574:SF40">
        <property type="entry name" value="WD AND TETRATRICOPEPTIDE REPEATS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15574">
        <property type="entry name" value="WD REPEAT DOMAIN-CONTAINING FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="43"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="165"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="893"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="131"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="358"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="754"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="964"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="756"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="791"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="964"/>
            <end position="977"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4374266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1079" mass="119850" checksum="8C4E7D213B86F5E9" modified="2023-11-08" version="1">MKYTVYDRLLRREVGQQRKKYTDIRGIYGDRQWVDDMDIVDELGGHHGCVNALSWSTSGRLLASGSDDHRINIHSYHPESSTSQFDLTTSIVTGHRSNIFSVKFMPYSNDRTIVSATDDVRIFDIEHSGHSALSSGSRRQTGRRSTRMAPDGVTLTEGDTNAKTFRCHSDTVKRIVTEDNPFYFLTCSNDGDVRQWDVRQPSKAYPPARDSMIPSWARDTDASDSVPPPLISYSRHGLDLNTVSCSPSQPHYIALGGAHLHCFLHDRRMLGRDRNRERGSRLSSPANWSEHDDALLGTATQCVKKFAPNGKQRMKRNDGGHITACKISDAYPNELIVSWSQDHIYGFDMLRAPDASEEVKQTKVANGDSSHRVKDQNRKRKRPKSGNSSQESAERAGSRQRTQSSDEDLALRVTYGNGQSEDIRIAPPAETRMSREELNDLRSTDHYRIAKTTITMAKRMFDLGDHGETGLRLAFTSILGFANSILSDMDDISRTWGYPVDPDPVDVAVQNKLRDSRAASRRFVQAAGTLARVLGGQLLTGANSDALISQHFASIQPAPRERELPRHEQFGYDFLKAILLWVDSGPGAVVEGFSARSSSPRLPIPQDSDIDAIDDILIPYLLEMATDDPIVNVDVSRFETDQTRVLFASGTDAVLAFSHALKVPFADLTGPSVPASCDSSTQTRDAAKRRWAYFVGRGLLLNAAKDIKFAYVDRAFGGRGIADKQMKVEEQTLREQQGEVDPSHDEGPVVDAELINRNSPGAGSEQTGASSSPSTGESVAHESRRIRVTTAEDDDAEEEDDDDDEDGDDTDGDVDSEEGDPEDDEDEDEEDEEGLRRTRSGRMLWRSDFGRSRYKRERVERDVPCAPHTRVYTGHCNVKTVKDLNYFGLQDEYVVSGSDDGNVFIWDRKTAQLVNILEGDGEVVNVVQGHPYEPTMAVSGIDDTIKIFSPDTLLQCNARKGIGVQSSDPNSFSSLNWSRRRRNQAQSAQSESEPSDETLSDSDKEVAANGLRSRKRMHQAYQITSKNDMDRKGGREDYFISQAVFAQLARHIAAQQAGGEGEEGEDGPVVITEENCAVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPD4</accession>
    <name>A0A9W9CPD4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_001827</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPD4"/>
    <dbReference type="OrthoDB" id="438545at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005290">
        <property type="term" value="F:L-histidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015189">
        <property type="term" value="F:L-lysine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015194">
        <property type="term" value="F:L-serine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF678">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="120"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="267"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="378"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="402"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="474"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="11">
        <location>
            <begin position="41"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="345"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="349"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4374751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="501" mass="54336" checksum="A0AA225C39EBC814" modified="2023-11-08" version="1">MATYSAIGPEPTEADPMSSSTGSLRGRRRGRGKKDAGFQGQATWISSVINLANTILGAGLLAMPSALSKMGIFLGIFVIAWAGITAGFGLYLQTRCARYVDRGHVSFATLSQLTYPNLSIIFDAAIAIKCFGVAVSYLIIIGDLMPGVVVGFAPGAGDINFLVDRQFWITAFMLIVIPLSFLRRLDSLKYTSVIALFSIAYLVILVVAHFIKGDTMADRGAVRVFQWAGPVSALAAFPVIVFAYTCHQNMFSILNEISDNSHFRTTTVIFASIGGACLLYILTGITGYLSYGDNIRGNIVSMYPTAAASTIGRLAIVILVMFSYPLQIHPCRASIDACLKWRPRRKNQSEGSPSRNSLMTNTPKPGSAQSREMSDLKFAVVTTVLIIISFITAMTVSSLEKVLAYVGSTGSTTISFILPGLFYYKISDPESPHHQRLVKNEDDEDEFQSGEVEGAVTSEAYGNRDWRRGLLRNLALTLSIYGLVVMVVCLVTNTFLVAAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPG2</accession>
    <name>A0A9W9CPG2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_001904</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPG2"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:001515">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF11">
        <property type="entry name" value="MONOSACCHARIDE TRANSPORTER (HXT8), PUTATIVE (AFU_ORTHOLOGUE AFUA_2G08120)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="292"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="477"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4374826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="496" mass="54212" checksum="5EC57CD30FF492F6" modified="2023-11-08" version="1">MTVDKAAHPSKRSQFNPYNFLVCWLVSLGQVAFGHPAAVIGTTLGEPPFYAYMKLVDAKGEVTSKAISLIGTMSGIFQAGAIFGILTTPTYSAELAPPGLRGFFVGMNGVNIALGYAIASYMGMAFFFASSDSAKWRGPLGIALLWPIMMIGITFIVPESPRFLLMKGRVEEAEKIVMRLHSIKGDPEQEFARGEFYQMLKQTELDRTLEPGWWAMFTKKGYRKRTALAMGFAFVGQSTGVLVINNYGPTLYATLGFDTKQQLELQCGWISVGIVFNAVGAMFMDKVGRKPLMLFGVGGCGVCLAIEAAIVATYAEAATNKAALGAGVAMFYLFLAVYSMGVDVAGVVFYSELFPNHIRAKGVCLSMATVALTDLVYLQATTTAFANIGWRFYLIFIIICALGVTFFIFHLPETKGIPLEEMARIFGDEVVVTLDDVHINHDTHELVIGHGEKGGLEHVATHQGVTPEIENAIREELDKEEKAGRKVHREDVNERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPI8</accession>
    <name>A0A9W9CPI8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_003690</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glycosyl hydrolase 12 (cellulase H) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPI8"/>
    <dbReference type="OrthoDB" id="89349at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013319">
        <property type="entry name" value="GH11/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002594">
        <property type="entry name" value="GH12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34002">
        <property type="entry name" value="BLR1656 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34002:SF9">
        <property type="entry name" value="XYLOGLUCAN-SPECIFIC ENDO-BETA-1,4-GLUCANASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01670">
        <property type="entry name" value="Glyco_hydro_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040872462" evidence="3">
        <location>
            <begin position="18"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4373395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="270" mass="29084" checksum="7F3A7727DEC6ED80" modified="2023-11-08" version="1">MFSEALLCLALVAPAFAAPAPQGITLSDSTKQQPITMCGTAQNIVLTDTPWIVYNMFYNQGQTKGSMCTGYKSVSTGSDGNKNIEWSAITDIDYVQATNNVPKGYTFVGLTQNLETKLSAISSIPATYDWTRTNTTAYKGNICFDFMTSDTKGDSTSSSAQELMLWLNYTGGQLPIGWTAGPKATISDLYGTSWKLYQGVNTDTGITVSSLLPDEQFTGSFQGDIKDWLLAMVKVGVFTESTYVNVGNAGTEPFYGKATVNATLGLQINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPL8</accession>
    <name>A0A9W9CPL8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPL8"/>
    <dbReference type="OrthoDB" id="2115692at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052523">
        <property type="entry name" value="Trichothecene_AcTrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791">
        <property type="entry name" value="GNAT FAMILY ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791:SF14">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="82"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="224" mass="25667" checksum="D03E763E2FD198DF" modified="2023-11-08" version="1">MPNPNLQISLLKPEEAEQYMRMRHEVFRPTVNNILYSRGEPSEKTLSRVTEEIRDGIVDKGILYIKCVDKATGEIIAGARWRHVKPGKEGAIERTWDEVEAGFDLPEPFDESHPEMLEGLHDLFNSNKREILGRRPYYCLDTLVTLPQHERRGAGSMLVRWGCERADEAGVEAYLEASLMGAPMYARHGFQAVKEVELDLRKWEGKEIMSFILMLRPAKGEQTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPT9</accession>
    <name>A0A9W9CPT9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_002752</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAP domain-containing ribonucleoprotein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPT9"/>
    <dbReference type="OrthoDB" id="445357at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052240">
        <property type="entry name" value="SAP_domain_ribonucleoprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040746">
        <property type="entry name" value="THO1_MOS11_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46551">
        <property type="entry name" value="SAP DOMAIN-CONTAINING RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46551:SF1">
        <property type="entry name" value="SAP DOMAIN-CONTAINING RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18592">
        <property type="entry name" value="Tho1_MOS11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAP" evidence="4">
        <location>
            <begin position="4"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="16"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="16"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="174"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="192"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="231"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="265"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00046328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4374013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="284" mass="30911" checksum="B428A46394A8BB74" modified="2023-11-08" version="1">MPEYAKMKNAELEALLKERGLPHGGKKADMVDRLTKDDEKTTAPANAKPADNPEDEIDWDDDIEVATEPVAKDDKPVTTNEETVAKAGGEGQPPNPQAVPNQVADIDPAKTDDLSVQPPTEGEQGESAEAQPEEKKEPAPDYSRGLAATNLDEEIEKRKKRALKFGTKIEDDEGLKKLERAKKFGETAPPKGLDEALPERTRKRGRDDGGEDAGGNKRRGGGRSGGRGGRGGRDRRDNRNDNRNRDGRDSRRDEHKSSGGSGWMSEKDRAAAEARKAKFAKPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ69</accession>
    <name>A0A9W9CQ69_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutaminase</fullName>
            <ecNumber evidence="2">3.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SNO1</name>
        <name evidence="7" type="ORF">N0V83_001231</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaminase PdxT/SNO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ69"/>
    <dbReference type="OrthoDB" id="2039at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903600">
        <property type="term" value="C:glutaminase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008614">
        <property type="term" value="P:pyridoxine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01749">
        <property type="entry name" value="GATase1_PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01615">
        <property type="entry name" value="PdxT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002161">
        <property type="entry name" value="PdxT/SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021196">
        <property type="entry name" value="PdxT/SNO_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03800">
        <property type="entry name" value="PLP_synth_Pdx2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01174">
        <property type="entry name" value="SNO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01236">
        <property type="entry name" value="PDXT_SNO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51130">
        <property type="entry name" value="PDXT_SNO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4375952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="284" mass="31763" checksum="4C9D727BD6FDE238" modified="2023-11-08" version="1">MPEATIITVGVLALQGAFSEHIQLLRQATADLSPQDLQWRLIEVRTPQELSQCDGLIIPGGESTTMSLVAARSGLLEPLRDFVKVSRRPVWGTCAGLILLAESANRTKKGGQELIGGLDVRVNRNHFGRQTESFQANLDLPFLSSSTSETSKEAPPYRCVFIRAPVVEKILPHKKGVQSEEERLDDTIVAPSKTPVDDLAREQLRSDVEVMATLSTRLPALQENQEHEHKAAEDIIAVRQGNVFGCSFHPELTEDARIHVWWLREVMQAMERRQQFELADRTRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRZ4</accession>
    <name>A0A9W9CRZ4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RTS1</name>
        <name evidence="10" type="ORF">N0V83_000415</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphatase 2A regulatory subunit B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRZ4"/>
    <dbReference type="OrthoDB" id="10264446at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051754">
        <property type="term" value="P:meiotic sister chromatid cohesion, centromeric"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901991">
        <property type="term" value="P:negative regulation of mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098813">
        <property type="term" value="P:nuclear chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000016">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002554">
        <property type="entry name" value="PP2A_B56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE 2A PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A 56 KDA REGULATORY SUBUNIT GAMMA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01603">
        <property type="entry name" value="B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028043">
        <property type="entry name" value="PP2A_B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="618"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="125"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="618"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="647"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="688"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR028043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4377588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="730" mass="81721" checksum="DF6C0062979CB812" modified="2023-11-08" version="1">MKGFRQRVSEQLSRNKDKDSKSSKKKDSTSGTASPSHGASNSPSGSAQATPSSSQTQLTDSRVKNQPAADGAQGNINSLQPGQQPASGSLAGGPQFGGQQGMMSPGSAGGAGASGPGTPSRLGQPLAPSVIISPSAPHIPPPGAAETMPGDLAPPKAGQKSLIFDRLQATPKDNIEGIRTPKRQHSSRFDISDQRQRELEKLPGFHEVPPNKREELFMQKIDQCNIIFDFNDASGDMKSKEIKRLALHELLDYVANNRQVITEKMYPRVVEMFAKNLFRPIPPPMNPQGEAFDPEEDEPVLEVAWPHIQVVYEFFLRFIESQDFNTNIAKAYIDHSFVLNLLELFDSEDPRERDFLKTTLHRIYGKFLNLRSYIRRSINNVFFQFMYETERFNGIAELLEILGSIINGFALPLKEEHKLFLTRVLIPLHKVKSLSMYHPQLAYCIVQFLEKDAALTEEVVLGLLRYWPKVNSTKEVMFLNEVEDIFEVMDPAEFAKVQEPLFNQLAKSVASPHFQVAERALYFWNNEYFCNLVSDNVEVILPIMFAPLYENSKGHWNRTIHGMVYNAMKLFMEVNPQLFDDCSHDYAESQNNAGQRQQSRQDRWDKLAKLAEARQNGTVEKVNLTSSTAASPMRVDDSDPLSQGRLENLRLQDDGSASRDRRPKEYERQSSQTSRRHHTQVEHHASSARRSAGRPRSNSRPNSSGNAVASARAPTGRARANSGGTITRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CS53</accession>
    <name>A0A9W9CS53_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000446</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CS53"/>
    <dbReference type="OrthoDB" id="426882at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053187">
        <property type="entry name" value="Notoamide_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47256:SF1">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47256">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="2">
        <location>
            <begin position="135"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="284" mass="32509" checksum="A9FE82AE7549901F" modified="2023-11-08" version="1">MKRKNEEMQGELSNLRQLYDFLRLRPEQEAMEIMRRIRANPPNTSPSQRIQELADFMGELVSAEDIRQIQTCQLACESTLPPPTSLEAILHPSPSSAMGDSSTDPRLLSAKNWTTVTGDINILVDLFSAWTTREHSYFHYLDREAFLDDMASGRTEYCSELLVNALLASACFHSDAVKDRQKPFSETSMTTMFYKEARRLWDLEEGKDSLTKLQAGLCLFMVLGKYGRDKVGHTFLLEACRIGRDLGLFQSQPLEASWSRSSETQRKWENVRAVTAWALFNFQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXP5</accession>
    <name>A0A9W8XXP5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Acetoacetate-CoA ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010482</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXP5"/>
    <dbReference type="OrthoDB" id="10253869at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030729">
        <property type="term" value="F:acetoacetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005914">
        <property type="entry name" value="Acac_CoA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01217">
        <property type="entry name" value="ac_ac_CoA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002937">
        <property type="entry name" value="PRK03584.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42921">
        <property type="entry name" value="ACETOACETYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42921:SF1">
        <property type="entry name" value="ACETOACETYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="7">
        <location>
            <begin position="46"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="111"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="6">
        <location>
            <begin position="585"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4362389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="692" mass="75865" checksum="84FABE5ADF776E12" modified="2023-11-08" version="1">MHATNGANGASTSSKLLWRHSSPQSTPMYRFLQLINQTYGLQLSSYQALHEWSIRNVDEFWRRAWDFVGIRHEGLPSSAVDARAPMFPRPAFFQGATLNFAENLLFPTQEVDPDSPAVIAVTEITRETVTWKELRERVKLCQAGMLGLGLKEGDRVAGYVANHTNALVAMLAAISLGGIWTAVSPDTGVHAVLERLRQIEPTILFADNAAYYNGRSHPVIPKVLEIARELPSLQATVVFPTVASVEIDVNLLSGSSAKAYDYATFTSLTSSTELTFKQLPPDHPVYILYSSGTTGAPKCIVHGAIGTLLQHKKEHIIHCSITPGSRLFYFTTCTWMMWHWLVSGLASGATLVLYDGSPFRYIDRDDPSTSIPDDLAMPRIIQEFGVTHFGTSAKYLSILEQKSVEPKAAGLSLDRLEAIYSTGSPLAPSTFSYVYSAFPSTINLGSITGGTDIISLFGAPNPLLPVYGGEIQGPGLGMAIAVYDYTGTDITSTGEPGDLVCTESFICQPVAFWGSEGSTKYKSSYFDKFTNTKNQPIWHHGDFVRINPDTGGLWMLGRSDGILKPAGVRFGSAEIYNVVLEHFPEEVSDALCIGRRRETDTDETVVLFLKMETGQQFTPELVQEIKATIKKSLSARHVPSIVDECPEIPVTTNGKKVEGAVKQMLCGLNVKTGASVANAECLDWYREWARTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ07</accession>
    <name>A0A9W8XZ07_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Acyl-CoA dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_009705</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ07"/>
    <dbReference type="OrthoDB" id="435240at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00567">
        <property type="entry name" value="ACAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000012">
        <property type="entry name" value="Acyl-CoA dehydrogenase fadE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000014">
        <property type="entry name" value="Probable acyl-CoA dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000027">
        <property type="entry name" value="Putative acyl-CoA dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF1">
        <property type="entry name" value="DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G06510)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016578">
        <property type="entry name" value="HsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="9">
        <location>
            <begin position="10"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="127"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="240"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4363412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="44030" checksum="E6FD494C27D34981" modified="2023-11-08" version="1">MRIMETTGFSETQLTVREAISAICSNFPNTYWQEHDEQGLDPTDFHAALAKDGWLGIALPEAFGGSGLGISEATMMMQTIAESGAGMAGAQAIHANVYATQPLAKFGTEKQLQETIPKIISGEFRTCFGVTEPNAGLDTLRLSTQAKKQDDGTYSITGQKIWITCAQVADRMILLARTKQREEVDKPSKALSMFCIPIDKSAPGLDMRRIKKMGGRAVDANEVFFDNYKIPADCLVGKEGEGFKIILHGMNAERCLLAGEALGLGYAALAKASTYARERTVFQRPIGMNQGVAHPLADAYMRLEAAKLATYHAARLYDASKTDDSIRQDAIGVAANSAKYLAAEAAFTACERAVLAHGGMGYAQEYDVERWFRECLVPRIAPVSREMILNYIGEKVLELPRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZL5</accession>
    <name>A0A9W8XZL5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Tyrosinase copper-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_009422</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZL5"/>
    <dbReference type="OrthoDB" id="6132182at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1280.10">
        <property type="entry name" value="Di-copper center containing domain from catechol oxidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008922">
        <property type="entry name" value="Di-copper_centre_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050316">
        <property type="entry name" value="Tyrosinase/Hemocyanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002227">
        <property type="entry name" value="Tyrosinase_Cu-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474:SF116">
        <property type="entry name" value="TYROSINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474">
        <property type="entry name" value="TYROSINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00264">
        <property type="entry name" value="Tyrosinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00092">
        <property type="entry name" value="TYROSINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48056">
        <property type="entry name" value="Di-copper centre-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00497">
        <property type="entry name" value="TYROSINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00498">
        <property type="entry name" value="TYROSINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040841866" description="Tyrosinase copper-binding domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosinase copper-binding" evidence="3">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosinase copper-binding" evidence="4">
        <location>
            <begin position="277"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4363968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="355" mass="38555" checksum="A745BCF825838208" modified="2023-11-08" version="1">MKVSSVVAATLLGVVGAAPLESRATYESDNLTKQGVVNLGFYVKKNGYPNPQKCTLDNTAQRKEWSTLSTAEKTDYINAVKCLAKKPAKTPAGIAAGAKNRYDDFVATHINQTLSIHGTGNFLSWHRYFTWAYETVLRNECGYKGYQPYYNWPCPDSCGVSLPPGNGGGCVKSGPFKDWSVNLGPVAPVLTDVKANPDFTGLGYNPRCLRRDISKAASQGWTKDSDVASLIKNSKDFLTFTTTMQGDFANGFLGVHTGGHFTIGGDPGGDLFASPGDPAFFLHHAMIDRTYWTWQNQDIANREYALAGTITLNNSPPSRNTSLDDIISLGYVGVPDVKIRDASNTLGGPFCYIYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZL6</accession>
    <name>A0A9W8XZL6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Luciferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009481</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NtaA/SnaA/DszA monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZL6"/>
    <dbReference type="OrthoDB" id="5561043at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051260">
        <property type="entry name" value="Diverse_substr_monoxygenases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016215">
        <property type="entry name" value="NTA_MOA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03860">
        <property type="entry name" value="FMN_nitrolo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011">
        <property type="entry name" value="ALKANESULFONATE MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011:SF41">
        <property type="entry name" value="XENOBIOTIC COMPOUND MONOOXYGENASE, DSZA FAMILY (AFU_ORTHOLOGUE AFUA_3G15040)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000337">
        <property type="entry name" value="NTA_MOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="3">
        <location>
            <begin position="1"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="345"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00033748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="379" mass="42134" checksum="7CAFEEF71E4BF6A0" modified="2023-11-08" version="1">MAAVTKKLSFGITASTTYENPFLLARRFATLDHLTKGRVAWNAVTSHLESAAKNLGLETQVPHDERYQMADEYLTATYKLWEGSWRDDAVVKDEKQYAVPGRVRYVNHEGKYFKTAGPLTTEPSIQRTPFIFQAGASTAGKAFATKHAECLFLAGMDPQAVRKSVEDIHRLAAEQGRDPKSVKTIAGISIIVDETDEKAQAKYEEYLSYADLEGSLTLFGGWTGTDLSLINDDDDLKFTGPGAIQSLVSTWSATIPGSDNVKWTKARVARELAIGGPHAKAIGSPTTVADILQHWVDESGIDGFSLSYAVNPGDYEDMVKWLLPELRRRGVFWEDYGASTTRENYLGDEKGPRLRDDHPGSKFKWTAEEKECRKRKLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0G4</accession>
    <name>A0A9W8Y0G4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009204</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0G4"/>
    <dbReference type="OrthoDB" id="3794773at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4364608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="319" mass="35531" checksum="33277A7BECE68F50" modified="2023-11-08" version="1">MPFSQKLADGFNLDTVTCEEFPMADWMQDDFVNGDGPPRNALRCVEGRQNSAAGSQWLSFREGTGQHSKRKCDGAMDQANMDCEYSVYERSKPDIPLTEPEPLDKFTVEMNLDNTNADLLGYCLATLPHGPSNIDPGEFWMDTLSLPNGANNFPLQNADNRYLIGAKHNDGWTQDFVWQCRLELFRQTGDNYDVGMYDAVDNSEVAKGSQDLPNDGDRLVLTPTNSKYPSLIVIRTGEMGKGGTPGSRIDFISSYTNDGSNPLRDFSFSTESQGSDTRFKKVDGSNPEKGFYCDVPNIDDSSENGPTQQITCYYPCNTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y142</accession>
    <name>A0A9W8Y142_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Alcohol dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008798</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y142"/>
    <dbReference type="OrthoDB" id="5407715at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051397">
        <property type="entry name" value="Zn-ADH-like_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677:SF4">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="2">
        <location>
            <begin position="33"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="1">
        <location>
            <begin position="197"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="40165" checksum="67A5E05468CABA6D" modified="2023-11-08" version="1">MSSPLPETHLALFRETTGQPLELKHLPTPQPVPGNVIVKILYVPVVSYTKEIQNGTRQYPYPKPFVPGPGGIGRVAALGPDATSLCLEDLVYVDSVVRSRDEPSDMYLLGAVQGGTAGSAKLARDMWRNGTYAEYCSVPLENAYRLDEEALLGGELGYKLTDLTLLARYLVPYGGLRDINVCVGETIVVAPATGAFGGAAVAVALAMGANVIAMGRNEATLHSLVEHFGARVKSVTITNDVGADTQALKKAASGPIDAVLEISPPAAAQSLHIKSAILALKKGGRVSIMGGIREDYGIPMNAVMRNDLSLKGKWMYEREDVKSLIRMVETGRLAIGERNGVKIEGDFAFAEWEKAFDVASENARWDAQVVMKPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y149</accession>
    <name>A0A9W8Y149_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pyruvate decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_009053</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y149"/>
    <dbReference type="OrthoDB" id="2867507at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02002">
        <property type="entry name" value="TPP_BFDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006203">
        <property type="entry name" value="PRK08327.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF164">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="6">
        <location>
            <begin position="5"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="4">
        <location>
            <begin position="208"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="5">
        <location>
            <begin position="413"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4364459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="593" mass="64411" checksum="93737EC34722ADA1" modified="2023-11-08" version="1">MVYTASFAFFEALWEYGVSHVFVNLGSDHPSIMEAIVKGQNEKKGQFPKIITCPNEMVALSMADGYARLTGKPQCVIVHVDVGTQGLGAAIHNASCGRAPVLIFAGLSPFTIEGEMRGSRTEYIHWIQDVPDQKQIVAQYCRYTGEIKSGKNVKQMVNRALSFAMSDPKGPVYLCGAREPMEEDLTPYQLQLEYWHPVEPTALPPSGIKAIAEALVNAQEPLVIAGYTGRNHAAVQELVQLADNIKGLRVLDTGGSDMCFPANHPAWLGCRYGNDPSIKSADVILVLDCDVPWINTQCHPKDTAKIFHVDVDPLKQQMPVFYLNAIQRYRADAYTSLSQLNSYLESNLKEKLSSQLFSQRWSTLQESHKKKLETIASEAKVDDKGHFGTSYLCSQLKKLCPNDTIWAIEAVTQTGLVADQIQATLPGSWINCGGGGLGWSGGGALGIKLAADAEGKKQFVCQIVGDGTYLFSIPGSVYWISQRYKIPILTIVLNNKGWNAPRKSMLLVHPDGEGSKVSNEELNISFAPTPDYSGIAKAASGGEIWAAHASTADDLGKLLPEAIKNVLEGKSAVLDAHLEGPEGKYGGGKGRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y155</accession>
    <name>A0A9W8Y155_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7143 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009945</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y155"/>
    <dbReference type="OrthoDB" id="2497581at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055567">
        <property type="entry name" value="DUF7143"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37592">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37592:SF1">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23631">
        <property type="entry name" value="DUF7143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040771416" description="DUF7143 domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7143" evidence="2">
        <location>
            <begin position="34"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="193" mass="20081" checksum="BA40F731D2FA9E2E" modified="2023-11-08" version="1">MYNHVTTALALLGSSYLTLSSAYAITPRQSACFVVGTTALPAEVADVVTSLGSSITCSATVKTLSNVPDVTSGAVSFSSIDFSQSTQTPLQFALSQFATADPLASTDLQTFQDSLNVYLATEAGIRSVGGSLAIKVPKFFLEMQVSRIETAQGNAPSEAALQVDHLRDKVTKNAAGESQALLDQVVALAAQLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2B8</accession>
    <name>A0A9W8Y2B8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Calcium/calmodulin-dependent protein kinase type I</fullName>
            <ecNumber evidence="7">2.7.11.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cmk1</name>
        <name evidence="7" type="ORF">N0V83_008278</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.17" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2B8"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000153">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002036">
        <property type="entry name" value="Gliding motility related CaM kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="23"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="330"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="362"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4365658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="394" mass="44052" checksum="EF22DD4C9BDF18C5" modified="2023-11-08" version="1">MASNGGSGAHTPQTQAQVQPCRYKTGKTLGAGSYSVVKECVHIDTGRYYAAKVINKRLMAGREHMVRNEIAVLKRVSMGHRNILTLVDYFETMNNLYLVTDLALGGELFDRICRKGNYYESDAGDLIRATLSAVAYLHDHGIVHRDLKPENLLFRTPEDNADLLIADFGLSRIMDEEQFHVLTTTCGTPGYMAPEIFRKTGHGKPVDIWAIGVITYFLLCGYTPFDRDSNLEEMQAILVADYSFTPLEYWRGVSLTAREFIRRCLTVDPSSRMTAHEALSHPWITELGKAPDGEEDLLPTVKKNFNARRTLHAAIDTIRAINQLRAGGAAGMMDGARSGEPRRGAPPSNIPQPAEDEDPMEIDSRGNGHGQTEEMIKEQERRIRETQQGLWGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2M5</accession>
    <name>A0A9W8Y2M5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycoside hydrolase 131 catalytic N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008210</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2M5"/>
    <dbReference type="OrthoDB" id="120072at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041524">
        <property type="entry name" value="GH131_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34612">
        <property type="entry name" value="GH131_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34612:SF6">
        <property type="entry name" value="GLYCOSIDE HYDROLASE 131 CATALYTIC N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18271">
        <property type="entry name" value="GH131_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040978462" description="Glycoside hydrolase 131 catalytic N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase 131 catalytic N-terminal" evidence="2">
        <location>
            <begin position="20"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="392" mass="40446" checksum="BF35E44B7DE79840" modified="2023-11-08" version="1">MFCKAAVLSALLSAASAGTILWDGRFNDLTSSADLEKWSFSNPVGPYQYYIHGTGSIDEYVNLDSTYKNPADTGSKQGVKITIDETSKWNGQTMLRTELIPQTSAAINKGKVYYHFSIKTAAENVPTATNEHQIAFFESHFTELKYGASGSSNTNLQWHVGGVSKWDVELVADEWHNVAYEIDFDAGSVTFWHSTGSNALTKTAGPFTASTSSNGADWHLGVLRLPGSNDGTGAEDWFFSGVYVESGELTTSVDSPGGSGDSSETVASSTAVAATSTAVVVASSTAVATSSTAVIVASSTAVAVVETPAASSVAASTEVPVASSTAASVEVPVASSSVVASSAPVASSAVVPSSTSVATPTASAGAGSNAVLPEEFTIKEFIAWLKAKQGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2U3</accession>
    <name>A0A9W8Y2U3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_009503</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2U3"/>
    <dbReference type="OrthoDB" id="3734019at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="325"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4364048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="683" mass="77210" checksum="06CF5970DBC45C89" modified="2023-11-08" version="1">MARLYRKIAKSVANAPCQASNIHGSLSQMIYDPQNAAKNQDVMLGHGLEPRLLLNTGERGQYLPLSPRDQAVNAVTSMVEETHHAFVLGDMSLQRHLAQIYRELRSNRDQLKVLTQVVHEERRSLQSQTDNDASNEGADASSSDVVDIFFDCVSSFSDDDSDDSLDRSRARFQGSADYLLERRANFIPEQYTTPGRFHSRIILISMLEIGFTNSYRLQRYCLFYLENPRVWRRVIVSATVVQPLRISTLAGISSPDTSNVFKVLPGNLKKQLEWALAKKSLPGSDTHVSLVISEPEPSLFEIEPSHVDVAVDDTQITTTVEAALLRQLNEVGCARFSEREVAVQLRLTVYTQVVQIYGQCLIEHKRPFTQTKHQTSRSDDERQKEFLNDVSILLALREFDAVIKFTGYVLDSTGSRLHSYLCEWPLLGSLEHVLTRALRTGQLIPWAIRETWVKQLITAVAKIHERGIAVGSINRLSVFVHEDGNLVLDNPKTARRTLNQSGRLPPELRSDSYYALSHAKRTLETDVFQLGLLLWCLMEHKVSASGLCPRFECTHKPKSSCPVHSNPIELPQSNNPMIPKYMDDIIRQCRERDPTRRCSARQMLWVLPRETTPVDATLLAAPLRDWEGFKWSVCDECGGFISDGIKFKCPICQGGNFDLCPRCVSEGVHCFVSEHILIQHVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2Z3</accession>
    <name>A0A9W8Y2Z3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arrestin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007945</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2Z3"/>
    <dbReference type="OrthoDB" id="3365616at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22952">
        <property type="entry name" value="ART10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF166">
        <property type="entry name" value="ARRESTIN (OR S-ANTIGEN), N-TERMINAL DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_7G02050)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin-like N-terminal" evidence="2">
        <location>
            <begin position="4"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="412"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="230"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="421"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="443"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="498" mass="54714" checksum="BB63055AE5F7365A" modified="2023-11-08" version="1">MLVSIALEHPHEHYTNLDVIQGKVFLRVPNPTNVSSIIVKLEGESRTRLLAPIHPNRPDKQRPVLEVHKFLYKTQIVWPANIRPEDLVNNPRASFTINAGSYEYPFQFKIPLNTACQQTNSPITNVSFSNAIPEFAKTPTQHIKATLPPSLAGFPGEAEIRYFVKVTVNRPQFFKENPRALANFTFLPIEPPRAEKTDGEAYARRQHQFLENAPSVAAGKRPSIFDRKSSGSGIPTSPTTGSVPPRVAIDARLPNPAVLTSNQDLPMRLLVRNVSPRTKNIYLQMLQIELIGYTKVRAHEVTRTESNSWILCSFSNMAIPVGSPTDAVDTEVPINPEYWSGQAIPNTVPPTFATCNLSRFYELEVRVGIGYGSYKHGEDQLVVLPLRLPVKVYSGIAPPKALLVAGKLNTTNLNVPPPQEQAPHTPTTPTHGGFGNSGGGFPGQPVQQQAAAPGYDEAPPSYEEAIGQDLPPINGYRGSYQPPPAPEGAPGFSDEKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y339</accession>
    <name>A0A9W8Y339_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DENN-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_008862</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y339"/>
    <dbReference type="OrthoDB" id="6019893at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032483">
        <property type="term" value="P:regulation of Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.200">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
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        <property type="entry name" value="dDENN_dom"/>
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        <property type="entry name" value="DENN_C"/>
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        <property type="entry name" value="DENN_Domain_GEFs"/>
    </dbReference>
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        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296:SF21">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03455">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00801">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00800">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="UDENN" evidence="5">
        <location>
            <begin position="231"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="4">
        <location>
            <begin position="864"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="60"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="150"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="719"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="776"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1080"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="719"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="776"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4365243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1105" mass="122410" checksum="DF37A9C0D70B46A7" modified="2023-11-08" version="1">MAPLPNTSTDASSTPLADYFFISGIESSQVYDERTQQAGIVTSPLAQPPVDETIEEDRALETASIRPTSQEGLPNSDSTRRRSANRLSEHRQSIGTIIGPDSKQSASNRSSATIKGVPVAGSGLSDFDFDVALRKFASERDNFLEEIQFTAGVAQQNRPTPTKPRPRPQRVPQGNIDDGLGNLRSGVGSIRRRISTMQSSLKRQSSVARQSSIRTSKRMSGYNSVIPAPQPFVPEPNMHPLLRRYEPVLLDRYPPKNMVEELKRRNPFPDYVPMFAFPNDVSVVSSDERPKSLWHGFAMTNGDGSKLYGICLIVWLPLNATAAEGLERQCEEWRRVHMNPEERELASSLGERLALERAKLSQLLTRLSGVAPESEERENLEDDIGAVEEKIQLMTDLLRPVRHGAASKIEGLTDGETGLWIPRAYGVMGRDASLTPFWKEWLKAVAVPMTNGAIVGVPASSPRVGMWQPIERYVVNLCAEALSPITSITQVEVAVRELRMYARKEAVNEIPGSRNIDIYALFRCLSIPNIVTLFEYVLSEARIILLSSHTAMLHLASMAITNLLYPFQWAGVFIPVLPARLVQTIEAPCPYIVGIERRYEPVDYPEDEYVLVDLDNDSIVSNGPPPPSLPRQQRRKLTALLQHSAPHHTQYGVPTGPPAYAVEAFPNSAFASENSGVFSHRAHPSTVQTYVGLNSTSFGTITGPSTSRPLIYNAFLQSSASSRGNDRPSTASTVKTFSNSPPSPKISPVSTVFPPMPSTPISRSDSGYALQANLREKRSGHFETSSRRSSSFGFERIPPMRRPSQPFLGHAPSGSTSSLSHELRAGSSYAPSVYAQSTLAASTIMPGMAIQQVRNTATTQWQEGHCMQFRPHEEKAVCSICEEKSDDGLYRCTGCCTFAHGRCVSNISMVCPTAFRPDQVRASFVRCFASLLYTYRRYLVPATGERKKAGMVYQFKMDEFVKSLPSENVQYITSLQQTQAFNEFIHERESKRSDDPSVKLFDEVILAKRNRGKSSFFYKSRVDFLSDTTDHLWRTASANPPNSRFPGDYRAVISRIPAKLDPTLMKEPRVIQGVPRIPMGKARRKPIPSMLGPGGPSNPPQIRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y344</accession>
    <name>A0A9W8Y344_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Macrophage colony-stimulating factor 1 receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CSF1</name>
        <name evidence="5" type="ORF">N0V83_009285</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10051416at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006113">
        <property type="term" value="P:fermentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029636">
        <property type="entry name" value="Csf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056779">
        <property type="entry name" value="Csf1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048636">
        <property type="entry name" value="Csf1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32085">
        <property type="entry name" value="PROTEIN CSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32085:SF3">
        <property type="entry name" value="PROTEIN CSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25038">
        <property type="entry name" value="Csf1_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21678">
        <property type="entry name" value="Csf1_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Csf1 N-terminal" evidence="3">
        <location>
            <begin position="141"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Csf1 N-terminal" evidence="3">
        <location>
            <begin position="580"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="domain" description="Csf1 C-terminal region" evidence="4">
        <location>
            <begin position="2347"/>
            <end position="3121"/>
        </location>
    </feature>
    <feature type="domain" description="Csf1 C-terminal region" evidence="4">
        <location>
            <begin position="3175"/>
            <end position="3243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="552"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1148"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1182"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2458"/>
            <end position="2485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2540"/>
            <end position="2563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3126"/>
            <end position="3166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="552"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="563"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1192"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1215"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1242"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2458"/>
            <end position="2473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3130"/>
            <end position="3160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4364688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="3244" mass="361472" checksum="BC7D11D2F062B322" modified="2023-11-08" version="1">MAGSIAAEPLDPRDGFNWVFLVELLICGILTIFFLFYFNRLFATLVSYGIRAYTWHTFHAYIDITSLQISPLGGRIFFKDIRYHAHNETILVHGGHITWNYWLRRVRDAGVYTDDEPPGDDESQQSPSTPDSRSASRSRNVDRAEKGGKQMPKQLPCRISIKLSGIEAFMYNRSPAYDGIIEAVRRKAEHEAKQDQTCNESVAESGNMHKDEEHHHHNKLKKADTGRSITAKLKKPELPAFLKLLPIHVDITKGAIVVGNEHTLAVITAQFEKASGTLDAGMCGPLDVYQQLFNFQVTHPVVHMKPNPDYKASQLDTAAHYKQEASQGASEESIIEEDAGRKRRRRWLTKLPSLSGLFTKSSDSIHTHAKSSGGADSKLFAPQWHFPGQERWKGLARYLDDSVNDGHGEWDGVEYAKTSLIADVPCVNVSFYWDVPGPVPLEIDNSRYIDPVRPNDINGSLPPDYGMDIQIFGGTINYGPWADRHRGVFQSMFFPGSHVDGVPTEALKPGDTRVLSVFKLYLGIEEDTVLRIPVREPSKDWKWKGKAHTLAAHDKPGTDKNKGKAKSRRRRGKHRDAAVAQNVRPFAWIDVKIASDSSVNYVMDMYASKSGFESKVDVDIKKTEISSSVNHGLLWRSGAIALDCDLSTPLGWDSLRKWLFNIKCQDLELFLLRDHLFLLTDLVADWGSGPPPDYFLFVPFQYLLNIDFTDFKLYLNTNDSNIINNPADLDDNNFIILYGKRLHGDVVIPLDKFRPLQNEIKFDVQGQALGLEILMPQKNTLKTFVQAPNVARLGGLTLKGSHTLMNETSAMNTDRLFMDIQGHRLDLELYGWLVHHFMKIKDNYFGDDMHFKTLEEFQGLPSVNLVADGTNPNGQPAKVSNDLDVILCISADQASVLLPANLYSAERSIRADLPYASADLRFTNYYMDLMVNFSPISVSLGGAKPSLDQSIEFTGGRTELFVDSTIICGHRLFGLPPSEPTYVCNWDFDVGRLSGECTSDFIETAAGAARSFAFTLDDDENSLPLTEAEVIHDATFLRLRTGEVHVWMHVAQEALLLKTSPISLDFNDLAGSTFSQKLKAHIPDLIVSAVDSKSASRHRTRAGEKHVVETHAFLQTTLSLDMLKRKLDFSDERSKQHLHMQVHDQRTNRTPFMFGDDPTPSHQRDTKNQNVRTPAMQYPDIPHPIFLFLPPSQDNGSFKSFPSSSDTTSRRGRPLRTNTSRSASSASLSNSIRSTRRHRTASKTSGSSRSRTLLGGSPDRFDREARFYTLPKEEERAKRNLPPSSVALSSSLATPYFPLDQVDPDLTDVPSFPALAQPFEERDVDGLEFSDVSTKHLDESFSHTSLLVRAERGIRVHCTPEFIRCVSKLIELVQPRRPEDILDDFQVKVMTKILDNRNRLEGKGSSIEFSVRIPFTHFRFQHGFDLQSAGDAGKDQYDVILNRLKTVARVKKYPGERAGENSLALHTTLGSLGVSVTERTRHGSNEDVAIQAEIKDVLLWMMQSKETSVNVAFRSFEAATASRKVDYLASLIHRTTLLADGFVANFIEVDQRQDHRLRYLAWYLTSVQSQYSDPAFLTKASYALRAARDHLRGHDSWKIISRFRYTWQCLPQDEKAGARDCSERSVECPANAEEKIIAMWDQWRTWDLAHVKKSLAMRLLFGRVTEKLPTPQTAPLHLDVKSASIKLIVDPGPQQSEVALRMLAINVALLPPTEPTGLMLVGTDTPKKSTTVQISSKESVLHIQWEICELAEALLALFQKENLRAAEKASSKAMTSSQTPATLYDHDFQVVFATDKAQIILDTINLRNTNTARHLQFSFIDADSKASGIGEVLTAHLHAEVAVSQLSSRSRSLATTQFQQPTLYFARVEHDKDTNVPDEIKAAAASRKIVVKVEEDVLGFIEVIDCVLKDEVAYFHRQLTSIQGASRETPKENKLKEKTTQLPNITLALFMDAYQIELALLHPLCWSITGSSGRIAVIPALGKTVSLRVDYDLEANLHRLYSNSSERSNIISSFELPPLNGRLTVTQNEKETKVLASGTIEKVQIQASEIQALATTMNKPEMSHMLQAIQDDVEVLKTHIDEIFPPSSEPRVVKAPSTSREILFGIQMTLSGMDIAASAPGQHPDSPTANLAFRLSSVQLKVTNIGSDQSIMVYPEAAIQIHEVTVDLSLTDSKTIRKCGNLTFSATFRATTEKDSDPARRVYRLRSSGLEVNVFADTASAVVDVMNHLQDKIKDLDLSKEKKYFRRLRHTKSRINQKRDKSPKDDANADDDSIDSGALFTSTYSLELLDLQASWIIGNSVAAFPRSETEDLVLSFGKIDLSTQKDDAARLMIEDMRLQTVPISANKRSRSLNSALLPKMVFNVAYASTKDTRKLAFQAAGKSLDLQLDSRFILPANVIERSVALAGKKFREASANWSMTPTTTGAQRKNPFGNKRLASLTVDANFAGAVVHINGNETVPVQGSSNRSQQKGRYSQFVGDGSKSSMAMRAPGVAVRVEYQDNGSDPSLTAEVRVDGSTNTLLPTVVPIIIDISDSIKAVVREKDSEGSPSSPEDSKAKPKPAAKLLEEDNIMSADPTTLLGRTRLNLGLRICKQEFSLSCQPIARVTAIAKVDDIYLTVNSVKSQEHGHFFAASAAFEKLQATVQHVYSRESTFGLDVDSIVLSLMNSKHVSGTSGISAILKINPTALQINARQLQDFLLFREIWVPPEIRRASKPPASPNHEPQEYLMQRYQQVTAATAFPWNANVAIADVKVDLDLGQAIGKSSLHIHNMWASSKKSTTWEQNLCIGVENIGIESTGRTSGFVELHGIKVRTSIGWPLQDTGVRRSPLIQASIAFQQLRVKTGFDYQAFLMADIANFAFLMYNAREEGEDAKDRLVAILDGDKVHVYCHATSAAQGLALWQAIERLIQENQHAYRQSLRDIEKFLRRKSSVSTAYSRSACQSQIITKPDNDTFKAPISLHTDVVVTLRSIKFGVFPSTFVDQQLLLLEAGDMQARFAVALETDKIHSSLGMTLGQLSVALSSVPTPKKSISVTELTVEDVVESTRSARGGTILRVPKVIATMHTWQVPRSNHIDYLFRSSLEGKVDVGWNYSRISYIRTMWSNHSRTLASRLGKPLPESNIKISTSQTLQDPSSPSTDTDPSTPSTTTRPRAPSLPSSDPQQQEKITAVVNVPQSKYDYTPLEPPLIETPQLRDMGEATPPLEWIGLNRDRLPNVTHQIVIVSLLEVAREVEEAYERILGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y345</accession>
    <name>A0A9W8Y345_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_007873</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CbxX/CfxQ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2423195at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd17936">
        <property type="entry name" value="EEXXEc_NFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06008">
        <property type="entry name" value="NF-X1-zinc-finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001660">
        <property type="entry name" value="NF-X1 finger and helicase protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000159">
        <property type="entry name" value="p-loop containing nucleoside triphosphate hydrolase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000216">
        <property type="entry name" value="Type VII secretion ATPase EccA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000160">
        <property type="entry name" value="WGS project CABT00000000 data, contig 2.55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041627">
        <property type="entry name" value="AAA_lid_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000641">
        <property type="entry name" value="CbxX/CfxQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050773">
        <property type="entry name" value="CbxX/CfxQ_RuBisCO_ESX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43392">
        <property type="entry name" value="AAA-TYPE ATPASE FAMILY PROTEIN / ANKYRIN REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43392:SF2">
        <property type="entry name" value="AAA-TYPE ATPASE FAMILY PROTEIN _ ANKYRIN REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17866">
        <property type="entry name" value="AAA_lid_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00819">
        <property type="entry name" value="CBXCFQXSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="1308"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="1586"/>
            <end position="1729"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="1858"/>
            <end position="1995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1167"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2090"/>
            <end position="2185"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="2204"/>
            <end position="2282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1196"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1231"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1241"/>
            <end position="1256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4366237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="2314" mass="257686" checksum="DB74A34DA2D7E610" modified="2023-11-08" version="1">MAEQEAAAKRAAGLTRLFNAVIHGRREMKSAADGDRFLEALCAQEDVSKCVECLIASPTGLIAVAKAFRFSRDSAFLNGQATCVIHHLSKPAVKQLYGGQFLHRILEQIVQPPTFWNTLVEAHNARILTSQGTHAFAWLLLEVLCVRSEDILDVRNVAERVTNNESLLNSSSLDVRNIGQKIKHILESTSDDSVEGPGGRHDNDFADYRKVKILPTPDEFASTEEPFYRRADVIASAEVANRGMMHLDNQFRLLREDMLGELRSDFQIATGQKKGRRKMILEKLRFAGIDCGPAKKRRPCSLKLLCSDDIPQLRNIKGVTARRKYISDNKNILKHQSLGCLVSNGSIVAFASVDRNEDLLAQEPSVAVLQVADPKSFGKALIACKTSWDLCFVQVDTAVFAYEPILKCLQSMTELPLEQQLLTLTPGSSEAVSGIQPSTIINAIRERWKENLQDVIGTDRPVHLDLAQAESLLTGLAKKASLIQGPPGNDEAILVLTYTNHALDQFLVDILNAGIPAASIVRLGSKFDANTRALSLSAQQSNYRRSGQTNAMINIQNGQAESYHDALTTKMADFSRASVNDQSLLDHLEFSDDSEYFDAFVVPKGKDGMILVGKKNKKIDKYYLLKRWSNGQDAGLFKANAMQEYPHIWNIDLQSRRELLAKWPKEMLEEQVAEIAKLADSYNTCQGRIRRLLREKDSYVLSQKRVIGCTTTAAAMYTEVIQKASPGIILVEEAGEILESHILTAMTSATKQLVLIGDHKQLRPKVNNYDLTVEKGDGYNLNVSLFERLVLNGVSHTTLNKQHRMRPEISTLIRSLTYPELEDAETTKGRAPLRGFQDNIIFVSHSKPELSAERIADRRDNGAKTSKENLYEAEMVLKCVRYLAQQGYGTDDIVILTPYLGQLFLLLRTLSKDNDPILNDLDSYELIRAGLLTPAGGNVSKRNIRISTIDNYQGEESDIVIACLTRSNSEGDIGFMAAPQRVNVLLSRARNALIMIGNADTFMKSRKGKDIWIPLMDQLKKSGHIYDGFPVKCEQHPEKTALLAEKEHFDSICPDGGCSEPCGKMLNCKTHTCPHRCHQLQDHSKMDCKAIISSKCSKGHTVTRKCHDKAAAACQKCEAEVRAQEKKRQRDYKLDQERQKKQREYAARLAEVEDEIEHQKRILKDQAEERDRQQALAQKKQDLLGLKTKAKETTRQPDVNKSSTKSSTIMSENNSPSQNGASTPQHPANPPTSKSSAATTSEHDGQPDWDKSEAKDDWEWQKKYEGAENEALNSLISMTGLETVKQQFLAIKAKVDTVVRQNVSLKGERLGAALLGNPGTGKTTVARLYAQFLVKVGALPGDHFFESSGSALANDGVTACKAHIDKILEEGGGVFFIDEAYQLVSGNSYGGKAILDYLLAEIENLTGKVVFVLAGYHKQMEAFFSHNPGIPSRIPIQMEFQDYSDRELQHIFCHYVNAKYKGNMQIEGGMNGLYVRIVARRIGRGRGRDGFGNARDVQNKISQITERQAKRLRQERRAGTMPDDNILTKEDLIGPEPSSVLQDNAVWTKLQKLIGLASVKQSIQSLLDGIQFNYQRELEEKPIVQYSLNRCFIGSPGTGKTSVAKLYGRILADIGLLSNGEVVVKNPADFVGNVLGGSEANTKGILASTVGKVLIIDEAYMLASGAAGDPYKVAVIDTIVAEVQSTPGEDRCVLLLGYKDQMEEMFRDVNPGLARRFPLESAFVFEDFDDAEIRCILDLKLKDIGFEATDQAKKVAMDVIQRARNRPNFGNAGEVDILLDRAKALHQKHMTAGKVKQFDTLEAIDFDPEFDRGERAATNLPALFKDAVGCEELIKQFQGYQTTAANMKALGMDPRDQLPFNFLFKGPPGTGKTTTAQKMGKIFYDMGLLSQAKVEECSATDMIGQYVGQTGPKVQKLLEKAMGKVLFIDEAYRLAEGGFATEAMDELVDCLTKPKYARKLVTILAGYDKDIDRLMSMNPGLTSRFPESVIFKHLEPNTCLELLVKVLADIRKRKKVPLDIHSIDPPSPDLHQRVLELFSELSVLDSWGNARDVKSLAKSMFGTLISSAVPPFDSLVLSEATVTHALESMLDERSRRSQAVGTSRFSERLPLHPLPQAHPQKQRPAPTLAPNLSTKAASPPPVVPQPAMIKTEEQKPLPQIEQSPPTNHEDGHGEDPISSIFNAKRDPGVSDATWNQLELDKHAMLAQERTYRRIQQEKREEEERIEEFKRMEQAAADEQERRVREAERVAAQLERRRKDQELAAIEREREMEKERQKKLRVMGPCPMGFMWIKQYGGYRCAGGSHWLGDGALGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y388</accession>
    <name>A0A9W8Y388_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Imidazoleglycerol-phosphate dehydratase</fullName>
            <ecNumber evidence="4">4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">HIS3</name>
        <name evidence="9" type="ORF">N0V83_007379</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y388"/>
    <dbReference type="OrthoDB" id="447729at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000005">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000001">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.40">
        <property type="entry name" value="Imidazole glycerol phosphate dehydratase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00076">
        <property type="entry name" value="HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020565">
        <property type="entry name" value="ImidazoleglycerP_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133:SF2">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00475">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00955">
        <property type="entry name" value="IGP_DEHYDRATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4366851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="234" mass="25758" checksum="7251E750E52B4BC7" modified="2023-11-08" version="1">MPRTCSLNRDTNETKIQVSINLDGGELSPYEHSDFWAALDQQSLNGEGEKIEKDHATQKSASQEISVDTGIGFLDHMMHALAKHAGWSLRIRCKGDLHIDDHHTSEDAFLALGTAFKNALQGSTGLARFGHAYAPLDEALSRAVIDLSNRPYSVIDLGLRREKIGDLSCEMIPHCLQSFAQASGTTLHVDCIRGENDHHRAESAFKALAVAIRHATTVVKQWEGEVRSTKGVLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y442</accession>
    <name>A0A9W8Y442_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_008135</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y442"/>
    <dbReference type="OrthoDB" id="10016252at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="4">
        <location>
            <begin position="37"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4366499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="228" mass="25963" checksum="48167CCD74CD2F46" modified="2023-11-08" version="1">MDGWDIQANFIVDPEHWFMAARISDDGLWRITYGEKPGFTRDQLRERLPEKFKTMLPGHPEPSEYKVANFAPYKVHQRLAKSMRVGRFLLAADAAHLCNPLSVISPSYELLLPIVLIWIDRSGGLGLTGGIVDVGNLYDCLRGIYENKADESILDQYNEVRRNIYLEIVDVVSSSNLKRLFDLDPDRAIEQDPFFQMAKKAETDPEFSKTLQNVSTSRGSANRLLVEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4D7</accession>
    <name>A0A9W8Y4D7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS10m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">37S ribosomal protein S10, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Mitochondrial ribosomal small subunit protein 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RSM10</name>
        <name evidence="9" type="ORF">N0V83_007439</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4D7"/>
    <dbReference type="OrthoDB" id="366214at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="30S ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_uS10"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_uS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="30S RIBOSOMAL PROTEIN S10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_S10"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2 9" id="KW-0689">Ribosomal protein</keyword>
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            <begin position="180"/>
            <end position="277"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
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            <dbReference type="ARBA" id="ARBA00022980"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035261"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042916"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00078476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4366910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="335" mass="38865" checksum="3CF575255189DC42" modified="2023-11-08" version="1">MQRLIRQQITQARRPYAHAASRTKSSKAEEADGGDGRPDKKGKLMDQLRRDLENDDVTLEQRLKDLRVDPAEYKSLWRSQQAANERMEEWEPRTREEAQQRWKDMQNTPDHLTLLHMYKRAGVEPIKVAKERGITLPTDKTPLDPKTQAALDELRSPINVRAVHLRPLRRTPEHGVPVCDLQLRTYSIRNLLLFADFAVRAAYYMGLPARGPIPLPRITERWTVPRANFIFKKSQENFERITMRRLIQIQDGHPDVVKAWLAFLQKHQYHGVGMKANIFEFESLEAATRAGYDIKEGDYTRRREGPVLQKVDEILGSDGFKEAMKGHKSELESRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4J9</accession>
    <name>A0A9W8Y4J9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007578</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4J9"/>
    <dbReference type="OrthoDB" id="4121058at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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            <begin position="45"/>
            <end position="71"/>
        </location>
    </feature>
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        <location>
            <begin position="131"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="173"/>
            <end position="183"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="298"/>
        </location>
    </feature>
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        <location>
            <begin position="299"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="322"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="488" mass="53435" checksum="DE554A088E29F136" modified="2023-11-08" version="1">MSVFAPPTRRDTFLYSSLLYADAGNSNHHPRASVAELAALLRPEAPNLSSSNKKRKSENTAAPPTTTKDPPWHFWSAQLIHYGLAGTKDKNVAKVRLLDKLNQGQLEVPGWVGKVEAELKKEWEAENRRLKKAAAGGAVAGEKGGKKQDKGEKEDKKGAKGAKKEDARGQGVGSSQSAYGSGVNVNVNLTLSSGFAMMEQQAGVRKPKLTPSKPQPTKRKRAASDVQEVPTSAESSRGAPKTKSEPAPKKQAKTKESTPKTQVRKESASKVQVKKEATPKTQIKKESAPKTQVKKEPTSKTQVTKEPASTPSRARVKKEPNSPRSSYPPSSYIKPDPYTNSSSQNPPTLLLSGKYDVRVPYDNLYSHCPFTLTLFRDSSHSNTWWGTISWGPLSGLIKMDPGPSDFDASGTTCSLGWRLRDIQDNSLTFGRKCTGEMTFFRDQTFSGVLDQMPHMGSVEMEGTRQPGPGVVGEFQDEWEDFVREAYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4M8</accession>
    <name>A0A9W8Y4M8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_007575</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
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            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
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            <taxon>Pleosporineae</taxon>
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        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:catalytic activity, acting on a protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
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        <property type="entry name" value="Cysteine proteinases"/>
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    </dbReference>
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        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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        <property type="entry name" value="Papain-like_cys_pep_sf"/>
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        <property type="entry name" value="TRAF-like"/>
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        <property type="entry name" value="USP7_ICP0-binding_dom"/>
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        <property type="entry name" value="Znf_RING"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
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        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
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    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12436">
        <property type="entry name" value="USP7_ICP0_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MATH" evidence="8">
        <location>
            <begin position="281"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="1354"/>
            <end position="1389"/>
        </location>
    </feature>
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            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
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    </feature>
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        </location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="158"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="174"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="980"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1071"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1093"/>
            <end position="1121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4367045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1402" mass="155923" checksum="460CD99F94A6D53D" modified="2023-11-08" version="1">MSVSVEQPLHFSQQPSSPSFSSPPQSPNHLIHAHTPAPPQPPPPPPADIDMSSSMTTVPPPGQRHDREDAEMQDGEGLPDMHVEEIATPPSNRESTTGSVAVEVAAVDEDAMDTAPDTDAALVLPNGSADPNEALVLAPASPALNGDGQAEPSSNEPALSAAHLDDAASQQLATGSWSVANSEQTQTENIPPIDPPPPNDPSQQPPPPPPPVEPVRSDSDSSDDDDGVQPWHPIQEDTSSPDEAELKEIEEMSEHSALDHEYWESKAFLPLEEPEYTAGETGRIHWSIDAYNGTRENPNREILMKSDPVTIGGHQWQIKFYPKGNDSDYLSVYLECLSVVDPKKGESGDQSEDTSKGEAKEETREETEEVDATMMASAPFAPTEPQHAPLPLLGSKTIPKRNCVAAQVSVVLYNPTEPRVNYSRTALHRFCNGSPDWGWTRFHGPYYDIPHRMRGQRQALLRDDKLAFTGYVRIIKDETECLWEHHSRDNPWDSFAMTGLQGLMLGEDASAPGGNMISAIASWMLFKPFRKLLYNVEVADPINEPFVRPKPLINALQKVLYMLRTQVDPGAGSVALDDVLDALDWYGIHERLDKLDVIEVWEILRSKLEEELQNTPHITILQDICGPKRDYSAGVPSYRVPVVGVHTMQEAISNSSGFIVPGQPLPQLLTIELERQHFDLKTRSYVKLLNKVILDDRIVIGDTHYILYGFVVHKQTLQSYVYQPILRPEGPGCRWYSYSDSKDENQVKCLTKRQAVDAHEGKPGTEHVVGNDAVAYIAMYVRDDTAKAAFVSDAESERWEVPEWIKVEVEKSKTSNLLPPMPPPPIEEPVVQSGTIDQPEEEGSEAPKTLEFQVVHSHTFAQHEGPGIFDAYDAKWLRGSPELIFKVQLSSKDGRNEIREKLVSVLKDIQDPRQVKFWFLDPIRGSFGRPQLLGTGKIEFSSGSYDRYSDLKDWKLGDSPFACRRIWVHVVDFDKLPELPKEDEKKEQSIEEPQSQTLMEGIPAETSSDEPQTDLPPPEVPPHSEDTPMSEPDESVPVPIELPALEIEVPPVPDTVEGGDATMSEVEVPQISDSMPSTTPVGDTEMGGTQEDLPPPPPPPVDIPTEPVQPPPPARSPSPEPPSDEIYFFLKFWNAEQQRLEAKGSHIALKSVRVDETVVTLLGLPLEDKKKIEMWEEEELTTTRLIKHRRTFSQLDLHNSTIIIAALPRTTEQRTALAARAAFADPQPYLAFRAFARNFPSKLNGHFTYTYFSSQHYKGEIKNGTPHGHGHMIYHSGATYSGTFRLGDRHGHGLYTFQNGDTYDGTWEDNQQHGTGTFVEAATGNTYVGGWQHDKKFGEGVTHWKNAQEAERLCRICWDEPAEAAFYDCGHVVACLACAREVQSCPVCRKRVLSAMKLYYVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4Y9</accession>
    <name>A0A9W8Y4Y9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Inclusion body clearance protein IML2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Inclusion body clearance protein iml2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">IML2</name>
        <name evidence="7" type="ORF">N0V83_006892</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Inclusion body (IB) resident protein that interacts strongly with lipid droplet (LD) proteins. Involved in LD-mediated IB clearing after protein folding stress, probably by enabling access to the IBs of an LD-stored soluble sterol derivative that acts as a chaperone in inclusion clearing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with lipid droplet proteins.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4Y9"/>
    <dbReference type="OrthoDB" id="2154985at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019412">
        <property type="entry name" value="IML2/TPR_39"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10300">
        <property type="entry name" value="Iml2-TPR_39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="370"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="150"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="264"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="180"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="202"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00043897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4367311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="731" mass="82461" checksum="57CBCFC4CA4D1135" modified="2023-11-08" version="1">MADDVESAEELLSKGSSPFHQVRALEEWLGFEQEVMREGLIPYPMSTVKLMLTLRIAASALSQSENSAYEHQRRANKDPNAYRSPIYPPGTEYAVCQAESQLMSAIVGVLNESLTEAIKSFYKLRKAYVTLENVMENEKKFLKERSMSSVNSIGSASSSRPGSVRSSMRGSTLKNSRPPTSAGISAAGSTTSFKQSSSQEKLTTKEPTVKPKVANDKDSDDEFDFVDAEEDRKGDETPVEYLGHLNLPAGKGASIALDSSRKTVDINSSSAPDLPSTENNPSNSTPDAIEDFEQLTLGDRDDPSADISTFSDHPVDQFILAGSNFCFGMLLLLLSFIPPSFASLLKIVGFKGDRERGMRMLWQATKFHDIHGAMAGVVLMGYLNGFTNFCDILPTTGEGSYPKERCTALLVAMRERYPKSHLWLLEEARMLGSEKHLEKSVQFMEDAGESPLKQLEALSWFERSLNSMYMHDYEKTAMSFQKCISLNNWSHGLYYYICGASCVELYRRTKTANPSAAKKHAAEAEGFFKQVIPNTGKKKFMARQLPFDIFCIRKMQKWEARAKEWHCDFIDAIGVSPLEEMIYFWNGYKRMRDDHLQVSLQNLAWSESSENPHWDKEGLDEKSILVLLRAATLRNMNKTAEAKALLEKDILPVDRTLFKGNLKDNWTAPCARYEMGANLWREADADGHPEEHAYMLDECRNWLEEVTKWDGFDLDARYGFRLPFWVLEPVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5B3</accession>
    <name>A0A9W8Y5B3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_006896</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5B3"/>
    <dbReference type="OrthoDB" id="1914839at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032936">
        <property type="term" value="C:SREBP-SCAP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045540">
        <property type="term" value="P:regulation of cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032933">
        <property type="term" value="P:SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030225">
        <property type="entry name" value="SCAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46378">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN CLEAVAGE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46378:SF1">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN CLEAVAGE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="369"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="439"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="562"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="12">
        <location>
            <begin position="303"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4367315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1109" mass="122803" checksum="97FEA8B5D5C32AD7" modified="2023-11-08" version="1">MIWYLLYPLRGTTEPPRLSTSHPIRRAFQAHGTATARHWLLSIVLTIVISVLLCYQAVFQTDSSAAAGLRNLPKHVWTSTTEIEGDRPADVVVRQVWVHGDYMNAIDLRVLREATYVQDVLIGSGFDRESTAHSNNPKLSSDPRGCVVPGPGEKWGWHSPLMYWDCSLSVLEEDRDLLGTINAHTRSHSALNITLRPSTVFAGKTFSNTKLRAADALVITVFDQTQSNLGDIWDSRSRQLATELQSDWTIFPRDGHVTRNRLYEFRFRPMTLSDDLFLAASYLVTAVYVILRMMQLRAVKSWFGLLVTIYDDIFRLINVVLETPPEMPPPQRIGNALGEVGHLSVAIAFQNLALLYLCSRLVTPWVADFCVFAAVTLVFDLVFHLTFFVAVLSVDVQRMELSDSLERTELTQPKKKSRQERQAWLSALREGTLPLSTRFAGTVAIFSIIIAINWHFFDSNDKHMSFKILRQKFLSRRYKRNNDSTWSLPPINQARTPAEWLRIQDHNTARELFGFIKPGAHSFVARIYDPLLVVSKGASGRDRPQPPTSLLEGLRRFTQAHAFPAALISVFLIAGVTLLMNYLLWTGLPEDVDEEEDQETILSVKTLPTQQTLDVVRLSSCAKGHLATISLDRSTAIWLHGRSGYLHTTLQTATMKPKLWPIYASAMDEGGSLLALCADTGQVGLWSTTASRFLMFPKVDIRGQVPILFAFTTLTTRTDAEKICLMIVSPDGYLTELEARTGIHHTKQITPKQIVCAQLYTCAKGNNSLVFVTKPGEVHILSLNDNSNSTTEVVAGLDPGPPPGSNPAKIQCIQGVPSLGLIFALRDEEAEVFDFNSRALIHSFQIGHIKPHSFRVMHSARRFCACGAPTVHYLSVAYAEQDTDHMIMQTFTQDDTATSQICLGKAPDREKYQCFGFDCAKESVHYVEPAGVWESTSALGVVGIRRGVGSTASPLAASDDGNPAADSFPLASALKQRARKQRAPNGLFSALDSAFSSRIQTSTTAGDADTWEAWTLSSTGEFYSRPLAPDDPEGTMESCAEDDLFVAAPGPISRLGKRSIAVGFGDTAKIITLGKESFDGSMNIDDGNLDIVLGSYKSRARKGNGRKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5I5</accession>
    <name>A0A9W8Y5I5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5I5"/>
    <dbReference type="OrthoDB" id="10257049at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08241">
        <property type="entry name" value="QOR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051397">
        <property type="entry name" value="Zn-ADH-like_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677:SF4">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="1">
        <location>
            <begin position="12"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="339" mass="36523" checksum="625E28B21515C311" modified="2023-11-08" version="1">MRGIQIKEYVKGPDDLKVLDLPTLTPKPDEYLIAIHASATNFFDLLQIRGKYQNQPPFPWIAGSEFSGVVLKAPSSLPGGKTPKFQEGDKVFGATQGGYATHVVATEERLKGVPEGWSYEDAAGVFVTAPTSYAGLVVRAGIKKGDWVLVHAAAGGVGLAAVQIAKAFGATVIATAGTKHKQDVARSFGADYAVDYRQSDWPEQVKKLTPKGRGVDIVYDPVGMIAQSMKCTAWNGRLLVIGFAAGDIEKMATNRILLKNVSVVGLHWGAYATNEPEMQDKVWDGIFELVKQGKYRGTAFTDREFVGLESVPAALKALGARDSWGKVVVKVPQEGESKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5P5</accession>
    <name>A0A9W8Y5P5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">3-hydroxyisobutyrate dehydrogenase</fullName>
            <shortName evidence="9">HIBADH</shortName>
            <ecNumber evidence="3 9">1.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_006589</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-oxopropanoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17681"/>
            <dbReference type="ChEBI" id="CHEBI:11805"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57700"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Amino-acid degradation; L-valine degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HIBADH-related family. 3-hydroxyisobutyrate dehydrogenase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.31" evidence="3 9"/>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5P5"/>
    <dbReference type="OrthoDB" id="21615at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008442">
        <property type="term" value="F:3-hydroxyisobutyrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000006">
        <property type="entry name" value="3-hydroxyisobutyrate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000630">
        <property type="entry name" value="3-hydroxyisobutyrate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002204">
        <property type="entry name" value="3-OH-isobutyrate_DH-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011548">
        <property type="entry name" value="HIBADH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="HIBADH-like_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015815">
        <property type="entry name" value="HIBADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01692">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22981:SF7">
        <property type="entry name" value="3-HYDROXYISOBUTYRATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22981">
        <property type="entry name" value="3-HYDROXYISOBUTYRATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000103">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00895">
        <property type="entry name" value="3_HYDROXYISOBUT_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0101">Branched-chain amino acid catabolism</keyword>
    <keyword evidence="6 9" id="KW-0520">NAD</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase NADP-binding" evidence="10">
        <location>
            <begin position="38"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyisobutyrate dehydrogenase-like NAD-binding" evidence="11">
        <location>
            <begin position="210"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000103-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU910714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4368233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="342" mass="36417" checksum="ECA449B6F626702A" modified="2023-11-08" version="1">MRTVSAVATPLRRLSPVAIRQGSRRCFSSSQNLQATWGFIGLGRMGYPMAKNLRAKIPAEDTLFVQDVNTAATKKFLEEHPQGVRIAANVREVAEKAETIVTSLPEPQHVKGVYKEMLEPATLLTDGFITKERLFIDCSTIDPMTSGAVAEIAHSTKQGKFIDAPMSGGVVGAQAGTLTFMIGAPHEAVEQATKVLSLMGRRVLHLGEQGAGLKGKLANNYLLALNNVATAEAMSMGIKWGLDPKALAGMINTATGKCWPSEVNNPVPGVIEGSPASRGYEGGFGLALANKDLKLALKAAAEANVKLAMGDAARAVYDAAEKDENCKGRDFSVIYRYLGGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6C6</accession>
    <name>A0A9W8Y6C6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_006611</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6C6"/>
    <dbReference type="OrthoDB" id="3923077at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF96">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="27"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="186"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4368255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="308" mass="33938" checksum="A5478E040E240A2D" modified="2023-11-08" version="1">MFAGCAIGGVKWGTGRHMDTLSNDDILKAMRLSIGLFLLRVTVTKTHRWIIYIVMGSTVVTGLVFFFVTLLQCTPISYFWNRAQNGSCVSIDVVIGLTFFYSGVSVICDFTFAILPMFLVWGLNIASVAVVVRLGYVMDFKNPDFLWATVDIAIWSDIEQGLAITAGSLATLRPLYRQLASKIGLTSTGPDPSKPTGMRTPQWNGAPSNESRKKSGFMSLTNTLLRTEKGTVTGTVGGNDEEYGMGDLQPIRLRDDLCNENSSEKSDKGFNTWSIQAGKISDEEHRAGTITMHNEIFQQSDRRSAGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6L5</accession>
    <name>A0A9W8Y6L5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Swi5-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_007551</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SWI5/SAE3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6L5"/>
    <dbReference type="OrthoDB" id="255837at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032798">
        <property type="term" value="C:Swi5-Sfr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034974">
        <property type="term" value="C:Swi5-Swi2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010772">
        <property type="term" value="P:meiotic DNA recombinase assembly involved in reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000709">
        <property type="term" value="P:meiotic joint molecule formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010760">
        <property type="entry name" value="DNA-repair_Swi5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28529">
        <property type="entry name" value="DNA REPAIR PROTEIN SWI5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28529:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN SWI5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07061">
        <property type="entry name" value="Swi5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="297"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4367021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="458" mass="48969" checksum="E7D1C25E3EC5EADC" modified="2023-11-08" version="1">MAIRQGRSEIPDSEDEPMTSSPANVSNEALDKLSPPGPAPSQDAQETRQAAKDTHQAYADHVGITVSGDIEGLNADRDNASMNIDASQFDHINTQLKDAALHERPQVETCDDELKPSSLGISSQPESSYKSALAVHDAPATRTMHPVEADLYCPDLRCEPTATVDQNLGEQSINAAFGDGTITSVTNSGDSSFASGLKDHTQQRSAKEPSASQADPAALIASLRASFADVPHTTEPMDVETNNVNKTKASVCVKEGETASPGIEDNTAKLERTLTPTLDNGNSSLSLQDPGHIEFQNSEHGNINDERGNQVQSNRGHNIDQPVAQSVNYGRMANGDRPKELTPRSTHSITQKTSQEITLAELKAQKAALLASLATLPAIQVLVEETASSQAEQSDGGEEPTETDIMAAASKIVKDHIKLLHEYNELKDVGQGLMGLIADQRGVRIVEVQDEFGIDAND</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6W9</accession>
    <name>A0A9W8Y6W9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoglycerate mutase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005891</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6W9"/>
    <dbReference type="OrthoDB" id="354304at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045820">
        <property type="term" value="P:negative regulation of glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043456">
        <property type="term" value="P:regulation of pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051695">
        <property type="entry name" value="Phosphoglycerate_Mutase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46517">
        <property type="entry name" value="FRUCTOSE-2,6-BISPHOSPHATASE TIGAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46517:SF1">
        <property type="entry name" value="FRUCTOSE-2,6-BISPHOSPHATASE TIGAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="311"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="228"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="37012" checksum="404319B8E1401F2D" modified="2023-11-08" version="1">MDLLRPSTNLCSAGSRDSELTNHGYQQATRLGTHFQSVGLSFTHLFSSHLQRAAKTAGKIREAQLGQLRDEALKRATPDVVQLPLLMEQDFGSMEGRKWYDRPPESKLSGKEHHRQENKETAGFVDVESKDSMARRADAFLDEHLLLLLEKSEAKELVVAVVSHGIFLSRLWKRLLLRLPPKSVALSPELQATARPSLEHLGGWSNTGYLELYLVKDVREQPSPTVHASPTPATEPSSSPAERAIADEQFGEVELPPSATAEVVTSLEGVAESPAPPVALGSSLSTSRIADGWTSTILTINGKDHLKGLKRTGGGVGSSRHDASQKSIETFFKRRKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y710</accession>
    <name>A0A9W8Y710_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y710"/>
    <dbReference type="OrthoDB" id="4001642at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000935">
        <property type="term" value="C:division septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053060">
        <property type="entry name" value="Cytokinesis_Signaling_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36419">
        <property type="entry name" value="ARRESTIN FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36419:SF1">
        <property type="entry name" value="RHO1 GEF LOCALIZING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="1">
        <location>
            <begin position="185"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="401" mass="44405" checksum="94FABC2B7603A207" modified="2023-11-08" version="1">MSAFVRVSGPPNSNFLVGYPGISATLPRIEGRVEIRPLAGISAPVNVSLVTIALHRRETIHPSAESITKKHLAAPRKEITDIVGKEMLLYRCHAGREYESILCMDLPFVIFIPYGRGGEEIARRVPPASLQLPSRTAETFYELVVTLQQGPQEQKKYPFPVPIQRYDTLSTFGMYNRPESAERVTDHLVTLGISLPRWSYGPLDPVSVYIKLSPNPDWLKKATKVTIQKITVGIDEEIIFNHEGDEPTRKVKTLARTTQAVGVKMPEAGYFTNLGLVFPAKDLRDSDGIIPRGKKEFPMYAVNGFTTTGTLYKIEYYLTVKAQMSSARDILLRQPIVVCPFDHAGCKEEMEAIEQAAKDAAHVTPDNPMLPASTIVRSNDPDGLRALGIAIVGGVRKPLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y764</accession>
    <name>A0A9W8Y764_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_005647</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y764"/>
    <dbReference type="OrthoDB" id="5343383at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4369883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="156" mass="17548" checksum="844597B0AC07C167" modified="2023-11-08" version="1">MRTPLKPGCLDPVMSSTEPFDVDRIISLWTKILQLQVDIGYYPNDDSISFPPPGGRAVDETICQEFGLTDEVLSLLKRLPCPSNFDEAYETTIFEESMAVPLTDSEWIRNSRDPARCWYADADAPLRSDLKPEELALVLVKDESGYNLILDTKASM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7K4</accession>
    <name>A0A9W8Y7K4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7K4"/>
    <dbReference type="OrthoDB" id="3793406at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="423"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="489"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="514" mass="58196" checksum="E44D778309586BE5" modified="2023-11-08" version="1">MRQLTTTMELVSSDDDSNPGADNSSDTTTRESGAAYLNNDVVPSKYGPGVCKFPNKTVVVNASLDLANTSLPNTRNFPPASFVSPSLPPPSDSMDYIDVSNAPSPPRASEHGDETEEATMSLSHSGTSGKRERQHSPLPYQQFRHNGHELISDLPFDPPKGPRGRQRPISPFEQARKDEYDSHSGFSFDTPGGPREPQRSPSPYRHIHHNERESLSSFSFDPHEGPRERQSVSPFEQVRDDEYDSPSGFPFDPPTGPRERQQAPSPHEQARHDEHNSSRPFPFDPPAGPQASSLICFDWYHKGHCRPKRRKGRWNCGMLHTLDVPDAQVSLPIYTIDHGPCGLKLCPLRDVESGSHWANNQTHNREHELPNNREPTFFEEGENFGGTKDDFKKQQRGIEDSRREDSIPPEDLALNRDEWMDLRRRNKREKNRQKRMAKRAGHRHPGPTPVYQSIRRRSSPVNETHHRRSSPMYETYVRRSSPVYQTHHQSSAPQSPRASPSGHRTTSDTSVIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y899</accession>
    <name>A0A9W8Y899_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006796</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y899"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="189"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="36"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="265" mass="30067" checksum="BC481ACF1412B315" modified="2023-11-08" version="1">MASNRYENEYYRLSPEDEDYTPETPSPSSADEDSSWADKSRRLRPRESRAATKPSLVVKLRYKRGSEQTNTIPLSSPIVTRTRGRRANVEATRKHLDSRGGSVQEQQHNEPAPASLSTPCTAGERGNRQDHEGLSAGSSKRKRQDEEEGDQELDYEKLVLADVAKNFEPSQLNEPYPPLEAFFKTQFQISEAQKEYDELLAAKPRTREEIKAAGAAQERLELLKSYEEELKKEGGFEAGIEYGMKLMLEKLEQLSGIQRENLDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8D5</accession>
    <name>A0A9W8Y8D5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cell wall mannoprotein PIR1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_006210</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8D5"/>
    <dbReference type="OrthoDB" id="5415592at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054508">
        <property type="entry name" value="PIR1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051153">
        <property type="entry name" value="Yeast_CWMannoprotein_PIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000420">
        <property type="entry name" value="Yeast_PIR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254:SF2">
        <property type="entry name" value="COVALENTLY-LINKED CELL WALL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00399">
        <property type="entry name" value="PIR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22799">
        <property type="entry name" value="PIR1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50256">
        <property type="entry name" value="PIR_REPEAT_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040958045" description="Cell wall mannoprotein PIR1-like C-terminal domain-containing protein" evidence="5">
        <location>
            <begin position="16"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="298"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Cell wall mannoprotein PIR1-like C-terminal" evidence="6">
        <location>
            <begin position="65"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="170"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF22799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4369126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="323" mass="32145" checksum="79A11546413B08B6" modified="2023-11-08" version="1">MKSFVVLALAAAAMALPQTSSPSGCSSSSSSSFQITTVNVTSSSSKREVEKRQLSGALTLTLKDGILKDQAGRQGYIAANYQFQFDSPIQAGAKETSGFSMCSNSSLALMGSTVFYSCNSGDFYNLYSQSTGAQCIPIHIQAVNGGGATQVSDGQPQASTPGAVVTQISDGQPQASSAGPVVTQISDGQPQASSPAVVTQISDGQPQASAPASSVPVVTQISDGQPQASAPAVVTQISDGQPQASAPAVVTQISDGQPQASAPAVVSQISDGQPQASAPVATSNLTMPNATTPSIPEFTGAAATGAASVGALAAGLLGLFALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8P4</accession>
    <name>A0A9W8Y8P4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004948</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8P4"/>
    <dbReference type="OrthoDB" id="3782133at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="34"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="207"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="288" mass="32381" checksum="6972A681F198EC6F" modified="2023-11-08" version="1">MTILLEDKPARSDIFAPTAKSRLLKTLVRHVSAPASSLPSPAGFDPSEGNLSISQKRSRPPLRPFSSSTPRSEQGLLSELSLAQSYTYNWHVHSQKHITDNILAEDTVKSRARTISEQQGQIIDLHHSNEGDPLRYKQLYTRFQAQVTKHNKLVDQLNDALRTINRLKKSDRAKGKVQQRNLRLKATLQHYTSRDGSSTSHLNGNVENTVLEALALANERIEQLESKGESLLDLLEEGKDGGQRDAGGDISERNVSEEILVAKVAFREILEDETFREQKVNWAELLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8Z0</accession>
    <name>A0A9W8Y8Z0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005484</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8Z0"/>
    <dbReference type="OrthoDB" id="4225365at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040900379" evidence="2">
        <location>
            <begin position="31"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="299" mass="34336" checksum="A95BA3D2A4CB9DED" modified="2023-11-08" version="1">MPRVTPYRILRLLLLPILLRLNEYTDKWNAANINVTNILEDANAWNAQQVQPKGDWMPYFSGPLKAPEIPAGNDDEEINLPWFAPADKHLFHYDMHGFTASPLLRAVVPEPGVVEVWSFSVVLKAMNSLFDHVSDDGLMKGYHKPEAWQTLYRTLEKNERSSGLLAIFKLPESAKLRDIVVKELRGEFPSRTYPIRRAMLFLLGPIFSIVFFLFGLLGLLRDSIAPIFPYIVLGLVVYILYRVWRYTSGSAARATKEKKPVWGPTGPMEVKSHSKWFDEEKELGVQRPQTVRLGRSWKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y983</accession>
    <name>A0A9W8Y983_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004592</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y983"/>
    <dbReference type="OrthoDB" id="5378679at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="375" mass="41866" checksum="C6145652D45E3407" modified="2023-11-08" version="1">MRLKTLNGAPLCECLDFNDTNLLKIDECNEFQKVTGQTETEDITKAFKWRRLTSQHTRLRTGWSQPYLPRSGLQTHQADLSFSIPNAHQDVTFPDGNTTNIDTTVTFDGQPSDTEGFLRHSIIFHDTLLSSQVLPDADADNTVASASFLTTSFNTSTTDSSSPSRVDGLNSTFQIPPTMAIMPLGSVPSPHHLKSIYPQTPTPNLLCVLMTNPERREVFVRKGGYKMHLWEVTVADDTRSGFKVSFWIRPPRESNNEQTNAQTLLLQALERVKVGDILLLRNIALTYFRDTVHGQSLNPSITRARTSIDILMKRNGASVGQASGLPGNIVERFNKVKRWARIHVATDSAGSKKRKARHSHSEHDESLPPDTMEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9D8</accession>
    <name>A0A9W8Y9D8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_006303</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9D8"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000006">
        <property type="entry name" value="General amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF6">
        <property type="entry name" value="AMINO ACID TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="388"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="418"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="495"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="8">
        <location>
            <begin position="54"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4369218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="568" mass="63298" checksum="032C28ECD2B92F4B" modified="2023-11-08" version="1">MDGIKYDDKAVNAHEKSADVSLEGQDGIYRNETLEIGHVDNVDQLQRHLGNRQVQLIAIGGSIGTATFVSIANGLVKGGPGSLFLAYTIYTCMLGLVNNSMAEMASYMPVSGGFIRMAGHWVDESMGFWIGWNFFLYEAFLIPFEISALNLVLTYWTDKIPVAAVVAVCIVLYFCINAFVVKWYGEAEFWLASGKVFLIAIVYSFTFITMVGGNPQHDAYGFRYWNNPGAFAEHITTGDLGRFEGFLGCLWSAAFTVVGPEYLSMLSGEVKLPRRYLKAAFKVTYVRFAIFFIGSALCVGIVIPYNESTLLDILSGDTGGAGTAAASPYVIAMRNLKISGLPHLTNALLCTSIFSAGNAYTYYGTRSLYSLALDGHAPKFLRKCTKSGVPMFCLCVTILFPFLGFLNVSSSSAKVLTWFTNIITAAQIIDSIVICITYLFFYRACKVQGLDRRTMPYFAYFQPYTTWIALIFLTMVVTCYGYSVFLPGQFAIDTFFTYYMMVLVAPVLFFGWKLFKRTKFIPASECDLVWQKAVIDAYEESFNEPAVGFWQEIGQIFGFRRNKKVDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9F7</accession>
    <name>A0A9W8Y9F7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004413</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9F7"/>
    <dbReference type="OrthoDB" id="2279190at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="92" mass="9625" checksum="39889B4D2D81E920" modified="2023-11-08" version="1">MSTLESTMRPTGYNSDTDSDGQASGGRKISSNIGPVPSSANLNRPAGNIIKGAVDDEGKLKDAALLIGIKLDLEAEIHLTARIRGDITIGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9M4</accession>
    <name>A0A9W8Y9M4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">alcohol dehydrogenase (NADP(+))</fullName>
            <ecNumber evidence="9">1.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_003844</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a primary alcohol + NADP(+) = an aldehyde + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15937"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:15938"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:15939"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.2" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9M4"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008106">
        <property type="term" value="F:alcohol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006066">
        <property type="term" value="P:alcohol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05283">
        <property type="entry name" value="CAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000158">
        <property type="entry name" value="Zinc-binding alcohol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047109">
        <property type="entry name" value="CAD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42683">
        <property type="entry name" value="ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="12">
        <location>
            <begin position="20"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4372071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="354" mass="37906" checksum="3301E152D3558538" modified="2023-11-08" version="1">MSSNTTSSDFKGWVAHDPSAADGNMKWGSYEPKSFAETDVEMEITHCGVCGSDIHTLRSGWGPSDYPLVVGHEIIGKVTRVGKDVKDLKLGDRVGVGAQSGCCESCGPCKIKQESNCKANTGTYNSKYPNGDKSFGGYAKSWRGPAAFAIPIPEDLPSEVAAPLMCGGVTVYNPLVSYGAGPGKRVGVVGVGGLGHFGILFAKALKCDKVVAISRSSSKKADALELGADDFIATGEDPEWMTKNASSLDLIISTISGSFPLDQYLNLLDVNGTFVQLGAPEDALPSFSPFSLILRNLKIAGSLIGSRQQIRDMLELAKKTDLKAWVQVRPMDEANQVLIDFEKGLPRYRYVLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9Q8</accession>
    <name>A0A9W8Y9Q8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9Q8"/>
    <dbReference type="OrthoDB" id="10654792at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="356" mass="39172" checksum="C23F29CBFE65DEC1" modified="2023-11-08" version="1">MDMNTAPRNSTFFFGSETSSVYSDDDLISETNSTGTQIHHKSVLRPTLGGIPRDYGQNFERSSTLSFPYENTPQDLDEVDIPDLSASQNRNAFYRFESAEEAQERHQAEAARAQVDENVRARHVLGSEGHIMSNGPDMLSYSRSVHSPHGSQINSTGVYAEEDVRGRTMSAEPSPMLPLMDAEEIANEYISEISSSTPSLQGEYPAHDLIVEATQPQIPRPRAISISRDPTELDLTDLSALNRQDHHDEALQALSSGTPNIVGSEDLAILPHSGIQASDETMNANRRQGKVDWREGLTNGSPPRASLFSSTGREYGRSNSARGYDSGEFITNLEDSAPATKKSRKRGVIKRLFCVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAF3</accession>
    <name>A0A9W8YAF3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhamnogalacturonan acetylesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_005311</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAF3"/>
    <dbReference type="OrthoDB" id="2141316at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037459">
        <property type="entry name" value="RhgT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43695">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_2G17250)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43695:SF1">
        <property type="entry name" value="RHAMNOGALACTURONAN ACETYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040915660" description="Rhamnogalacturonan acetylesterase" evidence="4">
        <location>
            <begin position="19"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4370789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="27510" checksum="DAF8E3CD1701263B" modified="2023-11-08" version="1">MKLSTIIPFTSFALGAFALPGGLNPRAAPMIYLAGDSTMAAKGANDGATDGWGQYLASYVTTTVVNKAIGGRSARSYSNEGRFTEIANLVKSGDIVVIEFGHNDGGSPTSTTDNARSDCPGTGEETCKSGKTGETVYTFGHYVKAAANTYIAKGAKVVISSQTPNNLWEGGKYSASAPRFVAYAAQAAKEVGTGASFVDHFQAVANMYSKLGNTATNALYPNDHTHTSPKGADLSAQAFAQAIKVALNGTTPISPYLKAGNPTVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAS4</accession>
    <name>A0A9W8YAS4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005804</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4370040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAS4"/>
    <dbReference type="OrthoDB" id="4692165at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="291" mass="33182" checksum="596328B241549027" modified="2023-11-08" version="1">MTVAVADKGCSSQGEDEEYEKYDTRDLPSPPSGSPIIRAQSPVKGVEKGDAPGSKDEWHLITNQTRIHRPLQIREMDHHLSDLSRKAMRPSLKEQLRAAFVPSTFDARQRSFLPSSRLRAIVTQQSVQIAFDETPKVSESEGHAIQSLTDWIVTRACKLFAILNLCGFESANLRYSIRNFKDNEYDDSRLPLENPRKQLPPSDIFPTPMWSPHTLDVFYEEQWRLLVPVFSSHTFEYNLQPDIILPFTVETSDSDTAKSSFVRKVEIHPDHGKDLATFTVSTNLHKPLDKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBL6</accession>
    <name>A0A9W8YBL6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005269</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBL6"/>
    <dbReference type="OrthoDB" id="2115692at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052523">
        <property type="entry name" value="Trichothecene_AcTrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791">
        <property type="entry name" value="GNAT FAMILY ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791:SF14">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="16"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="229" mass="26078" checksum="8E9C01D0565CE859" modified="2023-11-08" version="1">MQTEKTPRYPSNNMPLEIHPVAESDLADFVRVQMAAFSHGGGITGLLTPKPLPPDYVEKSIGKHIKSWREEPDVTYLKVIDTDLGGKMVAGAKWRINEKERTEEQMQSTLPVPGPDEEGRPGAQDFMWFLNSVRREYMGTKPFYFLHILATDPEHHRRGAGAMLIAWGTRKADSAQLPSFLESSPMGRPLYERMGFQEKYVKTWDLTKYGREGTDTSTVMIREPLLYVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBQ1</accession>
    <name>A0A9W8YBQ1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription elongation factor Eaf N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003348</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBQ1"/>
    <dbReference type="OrthoDB" id="125903at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019194">
        <property type="entry name" value="Tscrpt_elong_fac_Eaf_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09816">
        <property type="entry name" value="EAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription elongation factor Eaf N-terminal" evidence="2">
        <location>
            <begin position="19"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="258"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="370"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="476" mass="50932" checksum="14EF5864C5D403DE" modified="2023-11-08" version="1">MASPMVESRVEPHKKAHFSLHISDGIAKNDTSLGSLSDNHKPTQTSDSRTTKLTSSKKTYNLTLEDKDSSGAKDIFTFTGQKSAPRKSYVLVFDASSQQATLEPLSSSYTFNLTSKNNKDVSSQYPKIYPKKFSEGSQDKDEDDDLFGEAAGDGEAGDPDAANPYDFRHFLSKEKEKRGDESEYHFASSPDYRTGTGSAANTPQFGARRPTAAPVPKTKPAEPAPKKRKNVAPNPLVKPKKGQQAPPIHIERRATDNAPKPKGKAAAPPASKIKSKEIVDSSDDSDMDADGQVASSPPAPRRRSPSPVHRQPSDDEDEDAEGDSDDDGGLEIEVPDERPPRRGKGALASLGLGQNIGAGYLKSPSNGPMSLASAASSAAGSPNPQAFGSRNGRNAQDEIDFGDLGGDDAEGEEEEDEDYNMRDIEPMDIGPPARQGGHERKASAAGVAVEEDEDDLEKMMIEGLVGGDSSEESEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBT3</accession>
    <name>A0A9W8YBT3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">mitogen-activated protein kinase kinase</fullName>
            <ecNumber evidence="6">2.7.12.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">wis1</name>
        <name evidence="9" type="ORF">N0V83_003650</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBT3"/>
    <dbReference type="OrthoDB" id="10252354at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000433">
        <property type="entry name" value="MAP kinase kinase PBS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF25">
        <property type="entry name" value="MAP KINASE KINASE PBS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="279"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="300"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="598"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="640"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="83"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="107"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="188"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="222"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4373355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="682" mass="71453" checksum="470F5B1013053BC2" modified="2023-11-08" version="1">MASTASTPDADFDSDTPFDSPSPSSPPAPSSPVANASPTHLSADRPAVRSISTGGATAGMTPSSTPLGNINAARNPRPPNPPTISSRGSGLNVSQDILARIQAVHLGRQGAPPNGAAGRGPLPQTQNPNANSRGPLSPGGPVMGNPGGVPANFRMPPKPQMTSFQSAPAIPGRMGGSKPSLAEKRGLKLPGGLPGASPSGSSPAPPAGRKRPGLTLSQMNGDKPEDQAAPKKPETMLDKYSEYIDTKTGSLKFAGKAILNSDGVQFAGGTTFNISLDEVDTLDELGKGNYGTVYKVRHSRPRMRKPGQGLAGNKAAPGSPSRKNFDGDESSPTSAKPAAGTGIIMAMKEVRLELDDSKFAAIIMELDVLHRCVSPYIVDFYGAFFQEGAVYICMEFMDGGSIDKLYGDGVPEGVLRKITLATTMGLKSLKDEHNIIHRDVKPTNILMNTKGQIKICDFGVSGNLVASIAKTNIGCQSYMAPERISSGGIAQAGANPGGGTYSVQSDIWSLGLTIIECALGRYPYPPETYNNIFSQLSAIVDGEPPDLPQEGYSDAARNFVRGCLNKIPNLRPTYAMLLQHVWLAPLAKPETITEEDEEEVEAAHQAVGGEAAGDKGLPEPVEIYADKEVADWVKGAIEKRKKGTLKRAEKPALHAAPLDAVSSPSHNDTNGLDEALPATTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC45</accession>
    <name>A0A9W8YC45_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002606</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC45"/>
    <dbReference type="OrthoDB" id="422362at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="480" mass="52452" checksum="0CC89F3A84613CDB" modified="2023-11-08" version="1">MPCLRPLERTVTTRRATTASFTMAERDISPTSTSSTLSNITVQTDKQAGVRAMSSASPSISTPPTSLDDGASVLSETTKPEQAVDVDDGTPALQTMDEVQSTAHTTPATGNSEGRRSARSSRKSVTTYNVQILAGTAIHTPTKYLEKHHMNVLHGSLESIMQENVVTPVKKKPSKLRTAATEPSDPVEEQLVAEAAQAARRRTSSRVTDLRKEALRNLSGVGDAVANTISGGKAFIRGTLRRSVSEPHLKATQPSPGPVSLKRSRTVMEAEEKYQDEQMPAKEYLKPKSKVWLKQGLYVGQHRDFDPRLSESQNRVKKRNRKSKENATLPLPIFACDRLLNEDPRHVFRDFKLPFDTYSPLPRKVKVDGWVKLHKSKDHPPINIQSLIVLQTGLLATHRPCGNATSRILLSVTVTQKMVVGKPAIIVSWHTSVTALIVGLRLNDAATAHLQNSGDVPKVIAMTMALRSSTLMIAGMACAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCN9</accession>
    <name>A0A9W8YCN9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003894</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCN9"/>
    <dbReference type="OrthoDB" id="5377226at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="190" mass="21151" checksum="11E3D21E9F5DDCF5" modified="2023-11-08" version="1">MVYITSDLFGPTTTHDREHNPNPTFWQNMATAQYSAGLGKRKRHEEEERPSQYETSLRATQELRPNHSSNNHQGHHRLPPSEWVSHPQSFDPPPLGRDNTLAFALYSATMNAAASERRPVKQLKRHGPKAVLVKSTSHLMDIEPDLPPTVQLAVTDLRPCHTAIDATAEPAISVHESVLGDAGNQFARNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD68</accession>
    <name>A0A9W8YD68_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_002960</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cystine(out) + H(+)(out) = L-cystine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:66172"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:35491"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:66173"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cystinosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD68"/>
    <dbReference type="OrthoDB" id="75720at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015184">
        <property type="term" value="F:L-cystine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07822">
        <property type="entry name" value="SRPBCC_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000016">
        <property type="entry name" value="Cystinosin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005282">
        <property type="entry name" value="LC_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131:SF5">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040978480" evidence="13">
        <location>
            <begin position="23"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="293"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="400"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4374219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="419" mass="47189" checksum="0E0D683E57C673C5" modified="2023-11-08" version="1">MLLFSSMRVILILAFLVITALSEYTNLPDVPPGIFNASTRIEIHSTTTAAWNALTNFPDYADWNPFVRAAIVVSPQNLTLPEQYPVESNHLFMRTQIPPLPLPVNSSTPDNPLNTEFAYELITHVQPDLGRLAWKYTPEALLQSQRWQAISDLGNGTLLYESREVFNGPLASVLKATLGESLQKGFDAQGQGLKLLLEGGANSELVAFAKAISRVLGWAYFLCWSGSFYPQPISNYFRKSTTGLAIDFPTLNVLGFICYTVNTAAFLYSPTIREQYADRHPDSPETTVRFNDFLFAAHGAVYTLGYVKLLTVLVKYIPQAWVNYKRKSTIGWSIYPMLLDFAGGWLSLAQLVIDSSLQNDWSGVTGNPVKFGLGNITIVFDIVFILQHYVLYRHPKKQIEDEEEDWESEREELISERHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEF8</accession>
    <name>A0A9W8YEF8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kelch repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_002253</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEF8"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="656"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="703"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="749"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="703"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4375169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="797" mass="85221" checksum="B75A86B39BC3B9F5" modified="2023-11-08" version="1">MERVRPQPAANDNGRRMSVFTEVGLVDEERIRDERSPVRTHGDSLKRLRPAKMLRFRSRNDVINANGEKEDGDESDWESVCDEDTKMEPTSTPTVLPAQYTMSTKLYRLGLFSIVLALLLPIFQLNPLARVGVRGGAIPRATIQAVPEDSLLVKRDDSPTDACKRWSGQSTVVNGTLYMYGFREIDDAKQTDNTWTNNFLTLDLTKSWQVASPSLTGLPKPSGPPAVSLGQLWSSHDSLWLYGGQYSDNPIVSPGPNSVWEYNIGSKSWIEHTDPKTSAGDSAEGDGQSVQRAAEGAGFSVNTLGRGFYFGGHLDDFTTEGWSNQIARVYLKSLLEFTFPGRENNAVESLKTNKTAGADGVYRNITEGGLQNSGSFPERADGVLVYVPGFGDEGILLGLTGGDNDTFTQMNVIDVYDIAQSTWYKQSTSGTMPDYRVNPCATVAAAADGSSYNIYMFGGQNLQPAADQTQKDDMWILSVPSFTWIKVDQSNQSVPYARAGHACHVWDGQMIVVGGYVSKDISCDAPGIYVFNMSSLSWSDQFTALTGDNALKTYTGEGSTGNPLAQQANQRGFGVGAGIEGSYGYEVPAPVRSVIGGEATGGATLTAPVQTPTQGPLATGKPQTYTVTGPNGATITEVVGGGGGSSNGYNSKGTNVGAIVAGVIAGVFFIIAAYFAFCAWVYRKQVAIWKNHAAMVTARANNEKQGTYTSGSKNSSERPPRTLGESSGGAAAGSFDIARGTSAEQTAYAGAGGGGFGRRSSEGSVTDDLLSGSEPSFWGTRGVLLNPRRSLRVINRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEW8</accession>
    <name>A0A9W8YEW8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_002252</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEW8"/>
    <dbReference type="OrthoDB" id="39591at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051651">
        <property type="entry name" value="DMTF1_DNA-bind_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46380">
        <property type="entry name" value="CYCLIN-D-BINDING MYB-LIKE TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46380:SF2">
        <property type="entry name" value="CYCLIN-D-BINDING MYB-LIKE TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02178">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="491"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="491"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="540"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="15"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="610"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="163"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="217"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="237"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="345"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="623"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="711"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4375168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="788" mass="88397" checksum="DDC644B2D03DCA28" modified="2023-11-08" version="1">MDDLAASQQLIDEVAAVDRTTNNNGDSILPNGTISSTSSSHKKRKRKSSGASKPKEPPTPQPAASQVGWSPARQSLPGQLRSYTLADPNDFADSDEEHEAASSGWPVSPLKSHAHNISTPSQDDNVEVPDSQTVPVATAALMSPPMTSSVAVPASMPEESSTHKKSHKRKRKRKKVNSGNSLPAEEDNNRGEETAMATGEVLESPTSPTKGKRRHSNIISPSPSSQAEANHKETAPTRKKLGNKRQKMNGIEACDLLNSTRKPNQRVDDHSILDHEEEAQDDADKGHAESKVPGQKRRKKSGGKAKADAPAAATSGIDLSESLGYSSGEDEIPPVSPSESQGSAQKRKHTKVRKSARPSQTPRRIRTSNINPTNVLTAAERSLNTARDLNYPPDLRTSGDFTQDEDELIRRAIRDYQERKGLDTLELVELIQWTDDTKDISLSRRISDLVTKEVQDKHESNEFWEEIKDVNLTRSKEIVRSHIRSQYHIYKSGGWTKEEDEQLKNLFELYPRQWKLISKVMGDRSMHDVHNRWRDYVQYGDKRNTSTWSKDEENLLVRSITTVAQRDEDYRAAAGKRPLNEYTNKDINWMEVCTLMGNVRSRLQCTVKWTKMQSRNPPPRIQLEIKPRNKIVSEDEEDQTRVKTPKKRGRPRKSDAQQTPQSKRKRGRPRKSEDISIADTSTKQEKKRRKSRKSDVAAPESTEEHGGELQEQIEPDADAELDEGFDEPAIAKSSTSKKQKQKMSARESTSRSRPPSKKKSILSESRTTTPKSFKSAELIIDSDDIESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF26</accession>
    <name>A0A9W8YF26_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1993 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001331</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF26"/>
    <dbReference type="OrthoDB" id="3724345at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="entry name" value="dinb family like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018531">
        <property type="entry name" value="DUF1993"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36922">
        <property type="entry name" value="BLL2446 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36922:SF1">
        <property type="entry name" value="DUF1993 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09351">
        <property type="entry name" value="DUF1993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="DinB/YfiT-like putative metalloenzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4376050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="20631" checksum="1764F9A79531BB06" modified="2023-11-08" version="1">MTGITFHQLAIVPVLNELKNALAFVTKGYEYTKTQNIDPNDFLTARLHPDMADFIFQINRFTEAAKGIPRRVNSSIEDITFSNEEKTFPELIARIEKTIKYLESIDASSLEGRETAEVILLIRGGTLEVKLTGMSYVTQFAHPNFWFHVTTAYGLLRMKGVNLGKMDFLNGAGMINATPVAKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFA6</accession>
    <name>A0A9W8YFA6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Protein BZZ1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">bzz1_1</name>
        <name evidence="13" type="ORF">N0V83_001464</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SUN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFA6"/>
    <dbReference type="OrthoDB" id="5339822at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051526">
        <property type="entry name" value="Beta-Glucosidase_SUN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005556">
        <property type="entry name" value="SUN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31316">
        <property type="entry name" value="BETA-GLUCOSIDASE-LIKE PROTEIN NCA3, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31316:SF0">
        <property type="entry name" value="SECRETED BETA-GLUCOSIDASE SIM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03856">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040942607" evidence="12">
        <location>
            <begin position="18"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="96"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4376183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="459" mass="48853" checksum="38C5825ED9DF75F2" modified="2023-11-08" version="1">MKLGIAIFAAVAAVTVAKPHANVHRHAHGVAVRDAHPDNVVYVPAATETKIIYMLDGHSISEEEVRQGIANGTLMWGDDGNLSTSAALPVAVVTPTAAPDKHDQPAEASSVQAFQEPKSQEPTTPAAQPSSGSAPAPSSNATPTQQDTSSSSWADLVDNNGYCASCDKEFPNGTIPCSEFPYGYGALPLANEGLGGWSGIQDPNYRGSDGFDDIYTVRKGSCSDGSCCTAGNFCSYGCPNPYLKLSFPKRQGRTGQSVGGLYCNENGYLEMADGSIGTTLCGKGSSRMTVKVQNKLSKPVSFCRTDYPGTESMTFPLTLGPGETGFLANPDQSKYYFWEGRSTSAQYYVNKQGVPESEACTWGEANQGKGNWSPTIFGTSWDDINMHRGFSGLSQNQDCKQEQLDYDITFTGDAVVAPCRYQRSTNQYCQAGTCFDNKDQGCTASVGQGGTLILVLSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG41</accession>
    <name>A0A9W8YG41_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dual-specificity kinase</fullName>
            <ecNumber evidence="2">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">POM1</name>
        <name evidence="13" type="ORF">N0V83_001455</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14210">
        <property type="entry name" value="PKc_DYRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000112">
        <property type="entry name" value="Putative dual specificity tyrosine-phosphorylation-regulated kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.10.30">
        <property type="entry name" value="DYRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042521">
        <property type="entry name" value="DYRK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF22">
        <property type="entry name" value="DUAL SPECIFICITY TYROSINE-PHOSPHORYLATION-REGULATED KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="1073"/>
            <end position="1369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="165"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="487"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1368"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="86"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="214"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="342"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="409"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="444"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="459"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="535"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="546"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="627"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="674"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="692"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="733"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="760"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="792"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="825"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="848"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="865"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="915"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1377"/>
            <end position="1389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1487"/>
            <end position="1499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4376174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1499" mass="161954" checksum="356DBDF76E4569E0" modified="2023-11-08" version="1">MESTNVPLRSGSNVSAEDSSGSSEDRVRKFQKTADNGSRLRKTSAANALNAARPPPVSNNAAVRARNKGPLGPRGPPQDFSKQRIASGASAASAESRSFLPTTANEASTRPVGSNPRFSFSKQPEPIKTLGDSTGSYDFLPSVNFDDFANSISCEPDLSDFPAPGTFKSSLPASTSTPNMQSFTAAPADARPARSNSFIRRFSQSTSRKPSGASTTSNSTVNSDTSSMPPPTNNMAIRTRRQSQFPAQAPPNPAARAPRKSVGPGLLPTNSFEGRSEVARLAGDNTQPGLGRAPSLNKGTRRETFNTSINSAMSAPKAPNAARTAKAKSFQPPSRLQPGHLTVSSMTPDPSSMPFNQARSPGRSPAQRTHTPSSSSSKRQSTAHHISGLGARTISPTDARRLRRLSMNPNPPGVPAGPPTPQGDQSFDDRAFSQSPAMVFGKSMTPSSARATPEQNRKSYASGISLSSNSSLNSLKVANSAIPMRSNPVLAGSRLPTPKTRNVHSSAAGEEEEVPPVPAIPKAYESPKDPERSSFAFSTTSLKSNMPPPSAPPPSAPPPSAPPPETATNTIAHLPEPRHTETLAASPGKTGLANRSTGSIRHRRGLTVGAGSEPDRTPAAQQSSKKNLQPIRLPPLNLLPLGTPYNNRISSFPAPSAEVDNRNVTPPPKRAANKTPSTPMTASKASFFSRSNHQDQYEYSKHLRSSSSAFNLRAAEHAQDGAPTIGAMPIPTLNPTRQATTPFASNSLPKGSGEFARLQSRPSGEYSFSKNVEMQSMATAGSRPAQKDASDSAHTSTSTEPETPSSGSSLRRKLSLSGWRRASSKAASHATQHSQARKTTQKADDAAPQPPKHNDMPPPRLPASATWSGAIGTSPTPAATRSRPSLDFGRRKTSTATIASDTELEKPSARKATGNAGSRTLSGYTDQASNQHAHKSSILTPMQRLISSKSSSNVLKARHLESNLDRDDLAADEEMRKLASKRKDFEHAARDVDDLRKRAKPQERVTPAQALQMVNLNIFERGEIIDYKEIYFCGTKSAKKHVGDLNAQTANFGYDDDRGDYNIVLGDHLAYRYEVVDLLGKGSFGQVVRCVDHKTGGLEAIKIIRNKKRFHQQALVEVNILQKLREWDPDNKHSMINFTQSFYFRGHLCISTELLGMNLYEFIKAHEFKGFSLKLIRRFCKQMLASLVLLKSQKVIHCDLKPENILLAHPLHSEIKVIDFGSSCFETEKVYTYIQSRFYRSPEVILGMSYGLPIDMWSLGCILAELLTGYPIFPGENEQEQLACIMEIFGPPEKHLIEKSSRKKLFFDSLGKPRVTVSTKGRRRRPSSKTLQQALKCDDEAFLDFISRCLRWDPERRLKPDEAMQHEFITGVRRNPRQPQRLNSASVSSPVKRFPPSQTPQARTRPLPEPPATSFKNGTAVAAHRDASNSSSPVKAPAPQRRHSTAQPGQAGASGTKRAANGAPLQVSTGSGLPRAAQRSMSGKPDLASAAAIASLKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGE5</accession>
    <name>A0A9W8YGE5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Carbohydrate esterase family 5 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_001622</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGE5"/>
    <dbReference type="OrthoDB" id="2586582at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000675">
        <property type="entry name" value="Cutinase/axe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33630:SF13">
        <property type="entry name" value="ACETYLXYLAN ESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33630">
        <property type="entry name" value="CUTINASE RV1984C-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01083">
        <property type="entry name" value="Cutinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01110">
        <property type="entry name" value="Cutinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040785532" description="Carbohydrate esterase family 5 protein" evidence="4">
        <location>
            <begin position="20"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="265"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="272"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4376339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="346" mass="35783" checksum="83609BC0A92B00AB" modified="2023-11-08" version="1">MKSLILSTLTLLLATLTHAQERCDVPSPAIFCNTTEVLQYESSSCTPFHIFLSRGSDEPYPGRLGNITSEICATIGGTKACGFESIQYPAKSTAWGVDVWCKSAAKGAANGQAQMKAYSEKCPDAKLILLGFSQGGAVAQDVLGGGGGQVFACDQATNPALDASKAPGANIVAAVTFGAVVRNHGQNFTIGEGVNYDGRRARTPEQLAALNKYSDVLLDYCHYGDPMCAVGSEPESVEQHLNYFVQHNPEVIEWVAKMAKASEKGETVGKPSSSKSHTSSVVSSSSAKATATASASKAANNFAAASGTQAQATQSVVSEKTGAAAALGVHHAGVAVMVALVSAVML</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGK3</accession>
    <name>A0A9W8YGK3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Regulator of volume decrease after cellular swelling-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_000389</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGK3"/>
    <dbReference type="OrthoDB" id="19714at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034715">
        <property type="term" value="C:pICln-Sm protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039924">
        <property type="entry name" value="ICln/Lot5/Saf5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21399">
        <property type="entry name" value="CHLORIDE CONDUCTANCE REGULATORY PROTEIN ICLN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21399:SF0">
        <property type="entry name" value="METHYLOSOME SUBUNIT PICLN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03517">
        <property type="entry name" value="Voldacs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="214"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4377564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="253" mass="27383" checksum="AB12418952131FDA" modified="2023-11-08" version="1">MALQHLETAPKTEDFTPLQEHQEQTPTTFFGAKPVLYAQYSGLTLSSPASELEQNAAFSKFHSERDGEDALIKGVDIYVSSENLVFFQSTPSPTGVAIPYPSIALHATMKYKDTTDALFMNLSLNDVESVNDQDDIQVLGVTVLPPSYASNPDTACIQDIFGAMNTCADLHPDPNMSGDEGDDVLDETEPGATGWITADNMDEYVDEEGNFRGTVIGDEELGPGAGTVRQREDGGANGVNGDDEHEGKYYRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGT6</accession>
    <name>A0A9W8YGT6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_000301</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGT6"/>
    <dbReference type="OrthoDB" id="256333at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08234">
        <property type="entry name" value="threonine_DH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050129">
        <property type="entry name" value="Zn_alcohol_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401:SF2">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="6">
        <location>
            <begin position="53"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="5">
        <location>
            <begin position="182"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4377476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="353" mass="38230" checksum="C1D0F3A22AC2A8CE" modified="2023-11-08" version="1">MTEHNGDIDAASRQNKHGLDVPKKMKALQYSEAEKFAVVEIDVPEIGDDDVLDLHYHKGEFLARFPLIPGHEATGTIAATGRNVKNVAIGDRVAADALQACLSCFHCTRRKPLLCDSLLGFGGNVPGGFAEYCRYPARLVHPIGDLPDLEAVLVEPAACAAHGIERMQIEVGSRVLLFGCGPTGMLLAQLIRLNGAAHLTIASKGGPKLDLAKELGIADSFITISDHQPQVEMAALASANPYGFDVVVEATGAPTVLEQSIEFVRKGGKLVVYGVYDEGVKIAWSPFRIWENEITVLASFCSMGHMPHVLEYVKSGKLKLGGIVSKTYRVEEWAECLEVVRRQEVVKAAIVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH12</accession>
    <name>A0A9W8YH12_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_000319</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH12"/>
    <dbReference type="OrthoDB" id="1929172at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040999125" description="GOLD domain-containing protein" evidence="10">
        <location>
            <begin position="17"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="173"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="11">
        <location>
            <begin position="28"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4377494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="207" mass="23179" checksum="FC45B7ADC7C20DE9" modified="2023-11-08" version="1">MRSLLVIASLLAAVYATALTYRLEAHEKACFFAQVDNKGTKVAFYFAVQSGGSFDVDYSVVGPTDKVILDGTKERQGDFVFTANDVGEYRFCFNNEMSTFAEKMVDFEIAVENEAARAKIPSKQGSSPEQTSVLEESILKLSAQLSTISRNQKYFRTRENRNFSTVKSTEKRIFNFSLMESGLILTMAGLQVFIVKFFFQGARKGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH42</accession>
    <name>A0A9W8YH42_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000349</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH42"/>
    <dbReference type="OrthoDB" id="3357341at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="456"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="568" mass="64450" checksum="6CA8534413E4EF54" modified="2023-11-08" version="1">MLDENLPTFFLKASPDKIKHHESYYFTQNGSEPEAQYHLHHADPAANSARNTYAVALFDSHSPEILYGEVLAKPGWSHPTLSQEEIRKNGGVPPPPQPIYPTEFAIQLYNPDMQVIVRQQTTKWSSTVSWEFSIPQVTFRMPSSSDLDRTQSDPAADASTPRVNFVWRKEGRIGKDMTCYMTGKSTDPTGKKAKKSKEPDIAVALFSGMKEMTIMEPNLYRVEIEDYKGLEVTLLLGAAVIRDLFFSDLTEQFHINNTAIRKNSGGLRARKGSSPLEGPHITPPVMTPQPPPPPAYSGPSQYPPEKVPLQPASSNAVNTQIPPPSYTNNSAAKRSSLPPLATNQHRPNQPQPQLQRPVRQQQQPPQQPPRLDPRAQWEIDAETARLKAQVEAEAREEKRRADANRKARRKQEQEEERKTIQFLEQERKQKEREEREQRRKQAEIDKETERLRKQFGDQTSLLRPSPQQHRHSAPLIQGPWQTQRPNQQVPRPAPVPAQPPRVQNQAPFLQAPYLQPGGQRPAASQSSFFSNGLPKPQFQPKAKKSFWGLRTQSETNAEKLKKKKSSMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHL6</accession>
    <name>A0A9W8YHL6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ring-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_000501</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHL6"/>
    <dbReference type="OrthoDB" id="5398191at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="374"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="414"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="207"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="62"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="141"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="416"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4377673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="503" mass="54181" checksum="839B3E8698B91AC0" modified="2023-11-08" version="1">MASYLQKLRRHSKQLIPQTESKSADSTPQEAAKVSEPPKMEEATAGPSDPVKSPPAPTVTEPSDEPGAEPVLDEEDEKFLERLAAIASEPEGTPPPLPTRRMEVVDDTGEKKVGRDAQEALMDGADKVALPMSPPETTADASEKGKGKEKAKDGGLGRKKSVMSYFSLPKFNKKDGDKGKEKIAKDMKKKEVSDKDRAQFADDLHSAAEAAKQAELTEAQKEDKELTDILDQLNLSAVNNRVFSFSKESEELLNKFTQVLKDLVNGVPTAYNDLEKLFTDYDNQLKKMYGSLPPFLQNLVKSLPAKLTATLGPELLAAGAEKPGFDAKTAAGGSKSKRSMIPSVPSLKSLVTAQGAVATALRSIVNFLKFRFPALATGTNVIMSLAVFVLLFVFWYCHKRGRETRLEKERLAAEDAASGRVSSASSVNEDADSIFGDKNKTSKSAQNAKQGDEEAPKSPLIIGDGKEAGETTATVQDMPSVSHLPDPKDGQVPKPASPSPLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHT6</accession>
    <name>A0A9W8YHT6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000801</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHT6"/>
    <dbReference type="OrthoDB" id="529205at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="102" mass="11865" checksum="904264C128112D7F" modified="2023-11-08" version="1">MAPRALLPLRHRTPFLYTSRRTLSMTPRMLIKEDADRSPEQVEKAKQDQLKEQKQGEGRWREDLASHGESNIAADKEKVHDHEDHIEKLQHETKKKGESGDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHV8</accession>
    <name>A0A9W8YHV8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_000831</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHV8"/>
    <dbReference type="OrthoDB" id="5425637at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040876211" description="Transmembrane protein" evidence="3">
        <location>
            <begin position="18"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4378001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="17512" checksum="37C727D49E725A3A" modified="2023-11-08" version="1">MSRALLFSLLLPLLALADDTQSELPPAGYNGGTDNPQDPDDAGAAGTQKGAFSLSSGALAAIIVVAVIVVIGGIGSATLWWLAKKRQWDVRQSLRRASRRLTGRSTNDVSKQNRNNRRTGIRLNSPPPGRNANKPRQDRDLEKGLPLSNKEAKTTTTITSVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YI53</accession>
    <name>A0A9W8YI53_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">B-type cyclin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CLB4</name>
        <name evidence="8" type="ORF">N0V83_001574</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YI53"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20568">
        <property type="entry name" value="CYCLIN_CLBs_yeast_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20512">
        <property type="entry name" value="CYCLIN_CLBs_yeast_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000001">
        <property type="entry name" value="G2/mitotic-specific cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 4" id="KW-0195">Cyclin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="380"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="7">
        <location>
            <begin position="473"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="477"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="171"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="172"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4376291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="612" mass="69160" checksum="E43F6FAE431D9927" modified="2023-11-08" version="1">MDAKPQRPMRALRIRDENAPPQLPAGKVLHQRNKSTPALSTLMQVGGIKAAAAKRAVFADVSNTTRQVTAKDDMLVPSKKKNMDVLNESAIVSVKEVAKPNALLRPAQRPLSNITSQANVSNNTDTVASSIPKHVTLDIEQPAANIRKILSKKSTVFKELHVASIPETTIAPVPSRQPLSSQPSSFSNEDRASAPAQEPTPVTIEAVPEPSEHKVEAKYVEAVVEPEEKYEYLDALEEQARVLEQVRNEELAKSHALQNLDQEEYWDEDEEEEYYDADGYTTARSLRSRGDNTTGGVTLVLAPRVTAKTQRELEAARLYVEANRSPEDVEDEQWDTSMVAEYGDEIFEYMHSLEERMKPNSTYMDHQAEIQWSMRSVLMDWLVQVHNRFTLLPETLFLAVNYVDRFLSCKVVSLGKLQLVGATALFVAAKYEEINCPSVQEIVYMVDGAYTADEVLKAERFMLSMLQFELGWPGPMSFLRRISKADDYDLETRTLSKYFLEITIMDERFVGCAPSFLAAGAHCLARFMLKKGDWSQAHVHYSGYTLSQLRQLISIILECCDNAQKHHAAVYDKYTDKRYKRASVFVESEVTKGFQLPFVVRDSFASQTWRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YII4</accession>
    <name>A0A9W8YII4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Actin-like protein arp9 (SWI/SNF complex component arp9)</fullName>
            <ecNumber evidence="2">3.1.1.73</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ARP9_1</name>
        <name evidence="2" type="ORF">N0V83_000986</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.73" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YII4"/>
    <dbReference type="OrthoDB" id="74201at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030600">
        <property type="term" value="F:feruloyl esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4378153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="69" mass="7711" checksum="337F18141817A215" modified="2023-11-08" version="1">METPTSIKVVKPPEYFPEWKDVGFDESAFLGAQVAAKVVFVVDQGQSKGYMTRPDYNDQGPQGIHDYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIX0</accession>
    <name>A0A9W8YIX0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein HRI1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIX0"/>
    <dbReference type="OrthoDB" id="4045395at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11693">
        <property type="entry name" value="HRI1_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.320">
        <property type="entry name" value="Protein HRI1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031818">
        <property type="entry name" value="Hri1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043047">
        <property type="entry name" value="Hri1_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16815">
        <property type="entry name" value="HRI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4378176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="38485" checksum="E427C92288E3D1BC" modified="2023-11-08" version="1">MDSSGNLSTAAELASEQFDNNSDAASSCGSDSSQITVTPADSVSSMASSPEALALPGPAVPHSSAPGPVADSANISVREYIYFLPYPLPAGTPVPYSLDLANNNPLNLPPHQFEPTSTLVLTSPNRTFVDIRFFKPFAPAHSTPLPNQGGERERLEWAFAGTSASHAITLPETDNEEEESWEGVTHSTWTHWLDSRHAVGSSNIPVDEGDMYPIASDLTLEHGHAFQPMVNAYKTHEEMWRDVAARSTNSSGSKICVVMRLQADAAGVRGLIVRVGQYCQGIVMQGGYCTVERWEFDEGKGDGEEAAKWVRTARVGDQHLPCGTTFRPEVLAVGTVLKYHDYEWTVEEMWEWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CGW2</accession>
    <name>A0A9W9CGW2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Monopolin complex subunit Csm1/Pcs1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010128</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CGW2"/>
    <dbReference type="OrthoDB" id="2431049at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034506">
        <property type="term" value="C:chromosome, centromeric core domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033551">
        <property type="term" value="C:monopolin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990644">
        <property type="term" value="F:microtubule site clamp"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045144">
        <property type="term" value="P:meiotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23787">
        <property type="entry name" value="RWD_CSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.80:FF:000001">
        <property type="entry name" value="Chromosome segregation protein (Pcs1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040349">
        <property type="entry name" value="Csm1/Pcs1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020981">
        <property type="entry name" value="Csm1/Pcs1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038608">
        <property type="entry name" value="Csm1/Pcs1_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28006">
        <property type="entry name" value="MONOPOLIN COMPLEX SUBUNIT CSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28006:SF1">
        <property type="entry name" value="MONOPOLIN COMPLEX SUBUNIT CSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12539">
        <property type="entry name" value="Csm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Monopolin complex subunit Csm1/Pcs1 C-terminal" evidence="2">
        <location>
            <begin position="414"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="521" mass="56877" checksum="3B3054470D6B2C24" modified="2023-11-08" version="1">MAPRSALANISFTVDSASEDEMTHDELNALPTPDSNTENKAPTRKARGTTAQTKNSAPATKATAKGRPATRRVSGNSVLGAKKSNAAVKKNAPAKGGRKALAERKDANGSDTEEVDDFEEDEPAVQTKPAKRGRPAAKKAQVDEPTEEPAAAKKGRKAAAKEPTAKKEPKPKAVPKSRSTKRTAEPHEPEHMTIPETQPEPEADPMDIEESIEIDEIPESMPPPPRPSARRAQVPARTARQTSTGIRRTGSVSDSERDPVLRRKLGDLTRKLEAMTVKYDTLKEAASLGKESNFEQLKKKTEQIAKDQDAVIKALKQQNSELQSRTSDITSLKKELAQLSKENARLNSENKRLTDSLTSAQNESKTLSTKLAAARSAPSETKNVPGSAVKSRTTGGVLPGTAEAAKEAQLAKQKVDLYSDLTNLVVLGMKKNEDEEDVYDCLQTGRNGTLHFHLTVASGNDSYEDTEFIYQPLLNEQRDKELLDLLPDYLTEEICFPRGQAAKFYCKVVDSMSKKIILEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH37</accession>
    <name>A0A9W9CH37_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010786</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH37"/>
    <dbReference type="OrthoDB" id="2684236at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009836">
        <property type="entry name" value="GRDP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34365">
        <property type="entry name" value="ENOLASE (DUF1399)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34365:SF7">
        <property type="entry name" value="GLYCINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07173">
        <property type="entry name" value="GRDP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="844" mass="95560" checksum="0D59A34D32889899" modified="2023-11-08" version="1">MAEKKAQRTWKEKEMNDMRRESMKAFSVYAEPPPYSPQPPSAEVSSSNGATGGRPARSIPLIEHLSFTPSPLEIPTPAECIAHLKLLHAFAKLRHDVGNHEGLYFRTQKLGTEGHASAPESELQLNGHQQPGGSHTQDPSGAATEAHAAENTASTDSTHAERIRDKRWSIFVARAVSRYQEWWDSLSTAYQRSSSIWRTRITTDDFDSTQTTQYISKIPLTIGFRNADLYLVTKFPTEGDSLGHNSKFRLPPLDVLMVWHAHILNPRIYLEDCIRHSKHALWRTSFPWQRIYEAVDNDTFEYNQEDTYSFEKATGHYWDAHQDVSQTSKNIDCPQCATKLLVPWTRPPESGSQATMDSYLTSDTGYAAPAFQEECSKCRLIVTHEKLRVGKFIDDAYSLLNERRPLPGTILNIWGEPQCTTIGKNLSSHDAFFPNRVISKLSRFWPASLREEMKTLTVEKLKKMFQQVLKSKSDLTYVNSSQHKPEFVARGSKIAVRKLLSHYWDNSSIFGIDLVGAVLRQGTFVQKMHKLDWIHSPSYITTVQRLIVKYHRFVRLAALSPKQTVVPTLDVDLAWHTHQLAPPVYYRYTLAETKKFLNHDDKIPESNLHTAFQATSLAYEKKYGQPYSECSCWYCECTREPLRSNFLNKFSSRRSSLSVDKLDEKGFTKDAHLGPHVSAHNALVLDDEKTARERRRELEELDLQYAKVVKRYQKKKKGEEAPRRDNDAYLFGAYGYPMYYPMPIYVPYYADPSCTQEQYDKSAGGSGSSGGCVAATCAVSTSLGACAGGDGTPGCRASCGDMGMLEGVWDVWWRGWRGDGEDVVDVEGDFSPSLFLHGAMRMGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH56</accession>
    <name>A0A9W9CH56_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_010757</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat MET30/SCONB/SCON-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH56"/>
    <dbReference type="OrthoDB" id="19711at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="6">
        <location>
            <begin position="268"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="388"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="430"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="470"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="94"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="115"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4361816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="640" mass="70490" checksum="5083AFDED1185076" modified="2023-11-08" version="1">MHHRSSILSYRPKTSSAALDDDTAAAQNSIEHAMKRTASQTFNTQYHDMRETAMALDAVNEASLGADGIVSHHAHASSLSRAFGAVTGSFTRQKARRRLSSFSSAPSAPRRRGSIQSFIESLPSSSTGRKTSTENSRQPLQHQDSGIPTFQQATSMGAKSFSFKRLGSMRRRPTTSAFSEDVSKSTVAPSSIYPSPVLPGAAARQAAAAANQDRQNQLRREQEHTHRFLNGLIPTLRDPDIKDNESGVDMTCASPIVRTDSVTEKKMIDPFQRLPAELITTVLSNLDAASLVRAERVSRSWHEHAASPHVWRNVFLRKHEPEVHVSPTPIQMGGVGIGKMFDENKAITGSRDRTIRVWDLKTNKCIKVYVTPSVNGTRAGNDIYHVPADYHDASILCLQYDDEIMVTGSSDYTCIVWDITGDEFVPMYRLRGHEAGVLDVCLDDKYIISCSKDAMIKVWDRKTGTCMRTLKGHRGPVNAVQLRGNFLVSASGDGVAKLWNLETGVSIKDFPSEDRGLAAVEFSDDAKYVLAGGNDHVVYKFDASTGELVHSRVKHDGLVRSLFLDAFNGRIVSGSYDQSIQVSDYETGKTYAVYKNWTTSWILSAKSDYRRVVATSQDGRTLILDFGHNVPGSELLVGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH89</accession>
    <name>A0A9W9CH89_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">pectinesterase</fullName>
            <ecNumber evidence="3">3.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pectin methylesterase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_011009</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pectinesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH89"/>
    <dbReference type="OrthoDB" id="2019149at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321">
        <property type="entry name" value="ACYL-COA THIOESTER HYDROLASE YBHC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321:SF57">
        <property type="entry name" value="PECTINESTERASE 53-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SbsA Ig-like" evidence="9">
        <location>
            <begin position="16"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase catalytic" evidence="8">
        <location>
            <begin position="162"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4362067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="462" mass="50362" checksum="5440BAF1C3529B13" modified="2023-11-08" version="1">MRICTQRNVRTASVDLFPANGAKDVNPDTQLVLTFASPPIIGSSGNVTVYDNEKIIDTLDLSIPSSPSPYGNGSTKANYSDTTTYQSNLVGGMDFYFFPIIVRDNVATVYLHNNRLEYNRTYTVTIGSTVLTLSDGNSNGIDSNFSWTFSTKAQGPGSEATEVTVCADGSADFNTVQGAIDWAPSNSIKKRTIHIKEGNYEELVYFQYKSNLVIRGESRNKTIVGYPNNSAFNPPNRQGPSRRPAFSFKGVADLQLSGFSITNYYRGQAEALLIDGIRVVVDHMNLNGSGDALTTYGTAYISDTTLWGDGDTILGYGSVFWNQSTVISTAGAVSWTRTVQNIHGNVMLNCTIIGEQGNSTFARLPDNTGGVQPNWPYAEMVLLNTKTGGIAPEGWGPVQGAPFDTSHLHLWEYNTMDLDGKPISYSERLNVSRQLVSPEDDTFVEQYKNPAFVLGGWAPVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHA0</accession>
    <name>A0A9W9CHA0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_009817</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHA0"/>
    <dbReference type="OrthoDB" id="202203at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735:SF3">
        <property type="entry name" value="FERROPTOSIS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="5">
        <location>
            <begin position="6"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4363522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="419" mass="45455" checksum="B91CE709C19CA748" modified="2023-11-08" version="1">MAEQRNIVILGASAAGLQATHYILKHILPGLKAKKDAKYHVYCIAPSPHYYFRIASPRVATSTTLMAAKDIIFDLHEQFKQYSPNDFTFIEASASGLSTSGRTVSYRSNKGSQEEYITYHALIVATGSKTYFPAFSSNTPIQETLDAIASTNEKVKSAKSIIIVGGGPTAVEFAGEVGEHRNGKPGWFKNSERKVKTTLVTADKQLLPTLSASAGKKAEKKLNALGVDVLYNSRVSNASTAKDGRTVVTLGSGATLEADFYVPAYGVEPNSSWLPKELLNEKAKLITNETTSRVDIAGPRVYAFGDIASYSRNHIWDMLAALPALTVNIKRDLLSYDTHMPDAKPKGKDRLFKIDAQVGMIAPIGSGGGVGEVKGWTIPSFFVWLIKGRDYMVGMSGKPIATGDGMKKEFKWTAEESAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHS0</accession>
    <name>A0A9W9CHS0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_008988</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHS0"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000297">
        <property type="term" value="F:spermine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000082">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF38">
        <property type="entry name" value="POLYAMINE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="322"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="413"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="438"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="483"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="507"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="94"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4364394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="543" mass="58890" checksum="36CC4ABD0E1C7F02" modified="2023-11-08" version="1">MAWSGLTAAPTRPTSHVDSEKDKSLHGNLPVQSNAQSGAPLDPEKGIQPSPQRDAKSNSSTASPSKDRPVALDWDGPEDPLNPHNWSLARKWFQTYTISGIALVGTFASSVFTPGIEAAATELKSTDLIATLAFSIYQLGLAFGGPFAAPLSETFGRRPVIFISLPIFALFILGTGFAPNMAALIVLRFFAGFFAAPSLSMGSGTLSDIWPPEKRSGPMSLYVATPFFGPALGPLLGAAVTQTQGWRWTSWLILFFTVVLVIAPAPFYIESYKPILLRQRAKKRNLPLPRSPTEGMSLPKVVKTYVSKTLTRPMHMLLTEPIVATFNAYSAFNFGLLYAFFAAFPYVFKAQYGFDSLSTGLTFLGVGAGVIIGTICIVVFSKGYYQPHVRQAVSNEEPTPAFATSRLTPETRLPLAMVGGPCITIGLFLFGWSTAYRVHWIVPTIAEAFFGFGNMLVFMACMMYITDTYGPLYSASAMASNAILRYILGGTFPLFAVQLFRGLGPQWACTLLGCLSVVGACVPFGLARFGERIRKRSGYKRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHS3</accession>
    <name>A0A9W9CHS3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Protein PNS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PNS1</name>
        <name evidence="10" type="ORF">N0V83_010174</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Probably involved in transport through the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cell membrane</location>
            <topology evidence="2 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the CTL (choline transporter-like) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHS3"/>
    <dbReference type="OrthoDB" id="44736at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007603">
        <property type="entry name" value="Choline_transptr-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385:SF4">
        <property type="entry name" value="PROTEIN PNS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04515">
        <property type="entry name" value="Choline_transpo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="232"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="281"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="381"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="443"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="23"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4363056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="546" mass="60052" checksum="4838E5AB679AF855" modified="2023-11-08" version="1">MAQQGEAASYYTGGAPQQPQDAYYQGQKYGPPQQQSGQQQYTQQPPQYGQNYAPPQGPPPQNNGYAQQSYGDNKQGFEQTFKIAKPKFNDWWAGLLFIAVFLGYVAVSAISIRGYSQQNTGGIYGNANQFALNSNTVILFAFVLGMATILSYAYMWAARAFTKQFIWITGILNILFGFVTAIYMLSRKYWSGGIVMLIFSVFAAFCFYSWIPRIPFSVLMLQTAIDVSKKYGHTYVVSLIGGLLAAALGAWYSVTLVAIYVQYTPNQNNSSCSNGAGNCSNGKVIGLIAFVTFAMYWISEYVTSTLSEWLKNTIHTIISGVFGSWYFNPNSPPKGATRGAAKRALTYSFGSISLGSLLVAIIQFLRQLCSAAQQNAASDGNIVGSILFCVLGCLISILNWAVEFLNRYAFSYIALYGKSYIAAAKDIWRLIKDRGIDALINECLIGPVLTMGSTFVAYACALLAYLYLIFTSPAYNSDGGYTPVVVAFAFLIGLQICNIFTTPLSSGIDTLFVAMAYDPEVLIREHPDLYRRMIAVYPHVQQAIHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHZ2</accession>
    <name>A0A9W9CHZ2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010297</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHZ2"/>
    <dbReference type="OrthoDB" id="276276at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02883">
        <property type="entry name" value="NUDIX_Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43736">
        <property type="entry name" value="ADP-RIBOSE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43736:SF1">
        <property type="entry name" value="DIHYDRONEOPTERIN TRIPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="31"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="184" mass="20756" checksum="AD022D4AFA05DE7C" modified="2023-11-08" version="1">MSLSAQSLTYPSSLQEYAVSEQDFLRLHPEYQVLCTGIVVFNSEGKLLLVQRAVDEKAYPNFWEIPGGKVDDTDETILQAAARELKEEAGLEAIRVVRKVGQFTFSDGRPGRPAITWLKLIFEMEVRDSTNVVLDPVEHQKFLFASEDEIVKDLVGDVKLAYVSPPNKEVKLEAFRLQREALPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI38</accession>
    <name>A0A9W9CI38_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="2">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PIK1</name>
        <name evidence="8" type="ORF">N0V83_009892</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI38"/>
    <dbReference type="OrthoDB" id="10264149at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05168">
        <property type="entry name" value="PI4Kc_III_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000016">
        <property type="entry name" value="PIK1p Phosphatidylinositol 4-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021601">
        <property type="entry name" value="Phosphatidylino_kinase_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057754">
        <property type="entry name" value="PI4-kinase_beta/PIK1_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF22">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11522">
        <property type="entry name" value="Pik1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="7">
        <location>
            <begin position="623"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="117"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="425"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="601"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="154"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="436"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="487"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4363596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="905" mass="99167" checksum="5FCE314BC8A9C4E5" modified="2023-11-08" version="1">MSVCCWSVGVLTFGIQTFWLFQTYLHDLASAPNSIAFKTCRRIYNRVQRIVFGSAEPQRREKIKENILPVTVLSSLVLASIAAPFLPAHAGPLAIAQARKPRPIEDIISDNVQTAKVGRSKTVAGASPRLKQRRTAQGHSDPEDGRSSKPSSPRPREPRDPKELSRHAARPPNAPRAPSMQHRPSLLSVAHEARLSSSSLPDFRDPKRSPLPTPPPTAGLGPKPDHHGSRKLTSQPLRPLTPTALSRSQKIRLLRSNYFRNETQFLSALEDISNRLVVVPKPARLSALRAELALIAQDLPAEVDIPTLSPPTLKDGIASQSQHHRIVRINPAEATSLNSAERVPYLLMVEVLKEDFDFDPETEQNQQLLERLMLEKGTSRRRLFDITSAEYLALDRTPPNGTDSVFEPATGDLGSTSLIKDMDGNDNASGLARVTLPSTSANGKATAPRSSSGANTLSSMATLSTPRTSDSAISRSNSPAPHRRMTIATTNRGNQSVDQPDFSALATHMRTAAQMLAQLEASSAKRPKTEVAAIKAKIIASMQTLEEQNFLSEDQGPTFDTIMAESDPTTITAEPEDLEGGMVDATNSGAGAARMENDLKTGGMRRKGDRDDPSAATFGEEWSAKRERIRRSSPYGNMKNWDLISVIVKTGADLRQEAFACQLIEVCAKIWEEANVPVWTKRMRILVTGESSGLIETITNGVSLHSLKRSLTLASIAAGTNPRKRIATLKDHWLKTFGEPESESYIAGVDAFTRSLAAYSMICYVLQLKDRHNGNLLIDNMGHIIHIDFGFMLSNSPGSMGFEAAPFKLTQDYVDVLGGLTSPAFEEFKALSKKAFQALRKDAERLIMLVDLMSKQSKMDCFKAGAASVTNSLRARLMLHLSREEAEVFVDELIAKSVGSYYTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI85</accession>
    <name>A0A9W9CI85_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Blue (type 1) copper domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_009851</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI85"/>
    <dbReference type="OrthoDB" id="2331100at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00920">
        <property type="entry name" value="Cupredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052953">
        <property type="entry name" value="Ser-rich/MCO-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34883:SF17">
        <property type="entry name" value="CUPREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34883">
        <property type="entry name" value="SERINE-RICH PROTEIN, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040890617" description="Blue (type 1) copper domain-containing protein" evidence="4">
        <location>
            <begin position="19"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Blue (type 1) copper" evidence="5">
        <location>
            <begin position="22"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="142"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4363555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="203" mass="20613" checksum="AFC99699EB8A4A90" modified="2023-11-08" version="1">MHFSTIFAATALVGAAFAEDHTVAVSTKSGDLIFKPDSIKAAEGDTVTFKFWPKNHSIAQAAFASPCQPMANGFWSGFVPTTDTAAVANTTFTYEVKNASAPIWFYCTQGNHCNMGMVGVINPPTSGEKTLAAFKNASEAATGNVSPTSTAGTGGNLTVNGTTTSGSASSSASAPASSTGAASHLTGSVAFAGLTGLFTYFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIH2</accession>
    <name>A0A9W9CIH2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009598</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIH2"/>
    <dbReference type="OrthoDB" id="3034003at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040866141" evidence="3">
        <location>
            <begin position="21"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="659"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="624"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="709"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="762"/>
            <end position="785"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="785" mass="85999" checksum="EBD9515362BDB045" modified="2023-11-08" version="1">MSFFETFMLVLIAVAAVVTPLGLYEGIVAGPTSTAPAFHYIKDMSPMGYGTAPRDTNNTWSRLCGAFLLIPCPNDNNAATVVDNLTTISVDYQNDTYSSEVPKKVIDVFQSGLKNFNESVSSVFDIQFRNYVGSEVDDNEEGPIIDNRTSRTVGNYQPLSSLLLNDAIVPVEGLIVDMKNGGIGFRNHSAPTRKPYGSTWSEDILFVVPDTVCVDTNLTIDFVIPRTKSEGLKARSGVYRPSLTDRGGFINLNKTYPRWIVGDVQQNPELWYRAYRAAWLSNAYSMAYMNVTNFANETTGVKAFAYLNSEMNKNFPLYYPDNETATGGLSISPQMFSISTIFGSHLDGTEGLSNISTVGNQTTEYDFESKLPIYPNPFGVNSTQWSSIGEHWVYDIINILSLIHIPDTLCRGQGGGDLANITNIVSKCGVLNGAPIRNDGSTSLMFDPGSEWSVSMYTCMSTVQATIKTVSFRFNGTNDDLTGLEVVSLLDKTYPTEDPKPLWGVENSEMYLKDGNPLWGLVSEERSESLNLSTVRKASLYLPGRDGVMGWQNLPGVDFASIALDMAYNFAFTTKGVDYSGQSNLAMFRKWQELSKNPATSAKILNLIWTDIAANMVVGTKSLAPHENAKRKRDDATGDASTTPPVTNYTRRVKYHNAYGIPAFLALVLIVACFTSLIYFVLFSGATPSTMRTFLQHTSAGRLLTSHAGNAGHAQPHTGYDGYTPRGEGDGYSDTPTKVWLKGAGQRRFTLGGEGWTKNVQDVPGYDRKGGATATYAPVPNPQGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIR7</accession>
    <name>A0A9W9CIR7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Rhamnogalacturonate lyase</fullName>
            <ecNumber evidence="11">4.2.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">N0V83_009080</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-(1-&gt;4)-alpha-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end.</text>
            <dbReference type="EC" id="4.2.2.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the polysaccharide lyase 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.23" evidence="11"/>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIR7"/>
    <dbReference type="OrthoDB" id="114708at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102210">
        <property type="term" value="F:rhamnogalacturonan endolyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10317">
        <property type="entry name" value="RGL4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10320">
        <property type="entry name" value="RGL4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000102">
        <property type="entry name" value="Rhamnogalacturonate lyase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029413">
        <property type="entry name" value="RG-lyase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029411">
        <property type="entry name" value="RG-lyase_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016590">
        <property type="entry name" value="Rhamnogalacturonase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015364">
        <property type="entry name" value="RhgB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36574">
        <property type="entry name" value="RHAMNOGALACTURONATE LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36574:SF1">
        <property type="entry name" value="RHAMNOGALACTURONATE LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14683">
        <property type="entry name" value="CBM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14686">
        <property type="entry name" value="fn3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09284">
        <property type="entry name" value="RhgB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011794">
        <property type="entry name" value="Rhamnogalacturonase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9 11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 11" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041032943" description="Rhamnogalacturonate lyase" evidence="11">
        <location>
            <begin position="20"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Rhamnogalacturonase B N-terminal" evidence="12">
        <location>
            <begin position="21"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Rhamnogalacturonan lyase" evidence="14">
        <location>
            <begin position="278"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Rhamnogalacturonan lyase" evidence="13">
        <location>
            <begin position="362"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR011794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF09284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF14686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4364486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="528" mass="56013" checksum="2C89922ECBD8BEA1" modified="2023-11-08" version="1">MGFIANIAAALAFSQGATAAFGFTSTSKGFSVDTDGGLVFEINKSNGDLNSLKYNGVEYQGTGKASAINSGLGTSTVTAETVNGYVKITAKSSGLPVTHYYVAKKGDATIYMATNNQGPVDPGELRFLARLKTSEVPTGVHGDVGDTRGCTAIEGKDTFKCANGHTSCKMYTSDRFIDDKVHCVSGSKAQVCMVMPGVAYETSAGGPFMRDINSQTTDSDQELYWYMNSGHVRTEDWRSGLNGPFAMTFTAAGTTPSGNLDTSFFGSLKIDGYVGESGRGSVSGKASGVPDNFEAVLHWYNKDAQYWTKAQGGAYKSPLMKPGSYTMKLYKNEFLVTTDSVTVTAGQTATKDITSKEANTSTIWRIGDFDGQPFDLKNGDKITRMHPSDSRMSSWSGAYTVGSSKATDFPMALWSKQGSPATVAFSLTQAQAAKGHVLRIGTTLSFKGGRPSVKIGSWTGKDPGAPKLIDSRGVTRGAYRGYGEVYTWDVPASAFKEGANTLTVGVSGSGDAAWLSANYIVDALELQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIZ1</accession>
    <name>A0A9W9CIZ1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RanBD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10265837at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053074">
        <property type="entry name" value="NPC_Nucleoporin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38697">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN SIMILAR TO S. CEREVISIAE NUP2 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38697:SF1">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN SIMILAR TO S. CEREVISIAE NUP2 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="375"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="759"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="845"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="941"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1207"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1271"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="291"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="414"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <begin position="464"/>
            <end position="475"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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            <end position="514"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="524"/>
            <end position="555"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="570"/>
            <end position="606"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="616"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="797"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="944"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="959"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="977"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1018"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1069"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1096"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1126"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1140"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1271"/>
            <end position="1283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1423" mass="146811" checksum="0EEE10E187416B1B" modified="2023-11-08" version="1">MSGKRGNVFQQGRPENPYEENDDADIPQKATAAQLASRKIRTAKVKRPAGASSVSVSTPPAHYTPVIARARATATATSGMSANRYTVAGRREIRRRHEKQIADAHIQGLIPNVNFAPPQQSGFNFGASAPAPSNDSSNIGSNFNFGGASTFGGGATNSFPPAQSAAPSNPFTNSSFPAFGASSQSTGFDLQPPSAGFNFTAGASNPFASSNAAPMTNGNSPAPSFGGFGAQPQNNAFGGLGQNNTSNSASGGMFGTTSGPASGNMFGTTSGPASGGMFGTTSGPSQSTMFGTAGSPAPTSAPASQPFTFGTSLSTQNNTSASASTNMFSAFGTSVQAPTQNNGFSAFGTSAPGNAAATEIATPPPNLFGGFGTSTQDTEKTTSAPPATKLFGFGSNMPNNDNATTTAPKTDLLNTTFSAAPSTSAPSSTSASNPFANLSSTPSTTPALFNFTPTTQTPQGQEKNSTTSNNLFSSLKPADSGSSSAAETPKPNLFAALAKPNGTTNASSGASTPGPSLFGKAQNKDTASGSTEKSLQGATKAPSVQSSSMFNGFSTPQPPKTVGAAKPQGGLSNTTQPKPGTGIFSQTFQPENHKSSALFQPLSTPSKELPKERDTATQQAPKEVSNSFSKLSTPAAERSHLFNTAKEPMSASSASFMPTLTGAAPTTSAEAPKGAELPKIAKVHIPKEWAVPNVTAAQGINGLYNHVSELTMQLQALNERYRQQLSSLPLMADWSSISLWHYQHATEIKKKIDNAKKQRAAAKGITGNESTLSTKRKVNEESPENRNVSPSKRARPSEAPTTPTPQSSAPTSTLDPSATSTSNLFSKAISHKPSNTSASTNLFISKSSETPNSDPSKSAASVTGFTPLSSTTNGSKASEATANSAGFTPNLGGTSNITSKSTGFQPILGSASTGGAGGFFTQFSKTAKTYEELAAERKAKAMAEDYDSDDETEEQWSSRYDKKEAERLAAEKKQIAATPTFNVPNSTKSSGSTTPRSNPFAGLAKPATGASTPSLFTSRVSSPAPSVGGPSVLDAPSTASTPSPNIFGHLSSGPSSNNQDESDEDDDQQAASKSQNDQPIGSVESTTPPKRKYGESETESDETLEESMRRKKQDTASKGSLLSRMTKGDDGGVESEKENNNSASIFGQTNGTQTPTNKPFQFFDFGAAGSKTGSPKSDTFAGDQTFKAGTPIKFGFGDASATEKKDGAPKFVFQPATPSAAEFSTTPAKPPPTSLFSFGQSTSGSSLLPPHAGFSGAGSVASSVFSSRAGTPLSEADTSAASAAEEDEDGGKQEQVDFARLTEEELKALDIVFHAEVALAKHAVDKGDGSKTWANIAKGPLWILKDKVTAKCFVRIRIASGSTPLNYQILPALQASVTGSSKKQVVATMPRKEGGLSQVYYALKTPEIAEEFASKYNSSLPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJA5</accession>
    <name>A0A9W9CJA5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Glycerol-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="4 13">1.1.5.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">GUT2</name>
        <name evidence="18" type="ORF">N0V83_008887</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a quinone + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:18977"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.1.5.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.5.3" evidence="4 13"/>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJA5"/>
    <dbReference type="OrthoDB" id="264015at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004368">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.870:FF:000001">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.9.10:FF:000001">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.870">
        <property type="entry name" value="Alpha-glycerophosphate oxidase, cap domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031656">
        <property type="entry name" value="DAO_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038299">
        <property type="entry name" value="DAO_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000447">
        <property type="entry name" value="G3P_DH_FAD-dep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985:SF15">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16901">
        <property type="entry name" value="DAO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01001">
        <property type="entry name" value="FADG3PDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00977">
        <property type="entry name" value="FAD_G3PDH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00978">
        <property type="entry name" value="FAD_G3PDH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="5 13" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11 13 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="16">
        <location>
            <begin position="108"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-glycerophosphate oxidase C-terminal" evidence="17">
        <location>
            <begin position="521"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="735"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4365268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="756" mass="83055" checksum="0558B25E37F4C4F8" modified="2023-11-08" version="1">MAARRGSRILKPLLYTAGAGAIGSAALYIAVRPRDIPGTEAAAVPPPTYGEGGVFRPPQFPTAKSRDEQIADLKRSAGVVGIADKAKKWLGGSATAPPAETAEEEPYDLLIIGGGATGTGIALDAVTRGLKVALVERDDFSSGTSSKSTKLVHGGVRYLEKAVWNLDYNQYALVVEALRERRYFLDTAPHLSQWLPIMIPLQKWWQAPYFWAGTKFYDFLAGKENIESSYFLTKSKALDAFPMLKKEGLWGALVYYDGAHNDSRMNVSLAMTAALYGATVVSHLEVTSLEKDANGRLCGARAKDCITEMDGKKAEEFTIKAKGIINATGPFTDSIRKMDDQQVEEIVAPSSGVHVILPGYYSPANMGLIDPNTSDGRVIFFLPWQGNTIAGTTDTACDIKKDPVAGEDEIDWILKEVKRYLQPEINVRRGDVLAAWSGIRPLVRDPKASKTEGLVRNHLVTSSPSGLLTCSGGKWTTYRQMAEETVDEAIKEFGLKTQSLVNPTLISGTEVKDEAPLDGTCQTHRVRLVGAHGFSKTLFINLIQHYGIETDVAKYLCQAYGDRAWTVAALSAPTEQRFPVRGKKISGLYPFIDGEVRYCVRHEYAQTATDVIARRMRLAFLNAQAALEALPTVIDIMGEELKWDSKRKDNEWKNSVHYLGSMGLPKNKLALTRKDVESGHVGKYTDEDYHLYARHGESTPYAATQPSDDEACGQMFAFGIRLNQVFLDKPDELLESDSKYPAGHNPVVGKESKVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJK2</accession>
    <name>A0A9W9CJK2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Importin subunit beta-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PSE1</name>
        <name evidence="10" type="ORF">N0V83_008342</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJK2"/>
    <dbReference type="OrthoDB" id="543373at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041653">
        <property type="entry name" value="Importin_rep_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040928">
        <property type="entry name" value="Importin_rep_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041389">
        <property type="entry name" value="Importin_rep_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057672">
        <property type="entry name" value="TPR_IPO4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18808">
        <property type="entry name" value="Importin_rep_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18816">
        <property type="entry name" value="Importin_rep_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18829">
        <property type="entry name" value="Importin_rep_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25780">
        <property type="entry name" value="TPR_IPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="9">
        <location>
            <begin position="30"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="797"/>
            <end position="832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4365722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1093" mass="121308" checksum="21085457F2C06F8A" modified="2023-11-08" version="1">MSVLPAEVHTALASLLQGLQSPDNVQRTAAEQQLNDEWVSQRPDVLLMGLSEQIELAQDTSTRTFAAVIFRRQSSKPRKTPSGQTADLFLTLNPAEREAIRAKLLSCLAKEQDVSVRSKVGDAVAELARQHTDEGVPWPELLGALFQASQSQDAAQRENAFRIFSTTPQIIEKQHEDVVVSAFKGGFADSETSVRIAAVEAFASFFRSITRKAQSKYYSLIPDILNILPPIKDSGDAELLTKALISLIDLAEVAPKMFKPLFNSLVQFSISVIQDKDLGETARQNALELMATFADNAPQMCKKDTNFTNDMVTQCLSLMTDVGAEDDDAEEWNVSEDLDEESDSNHVAGEQCMDRLANKLGGQAILPPTFNWLPRMMTSSAWRDRHAALMAISAISEGCRELMVGELDKVLDLVLPALRDPHPRVRWAACNAVGQMSTDFAGTMQEKYHQVVLPNIIPVLESAEPRVQAHAAAALVNFCEEAEKNILEPYLDQLLNHLLMLLQSPKRFVQEQALSTIATVADSAEAAFSKYYDTLMPLLFNVLQEEQSKEYRLLRAKAMECATLIALAVGKDRMGQDALNLVQLLGRIQNSVSDSDDPQASYLLHCWGRMCRVLGREFVPFLAGVIPPLTELAGAKADIQLLDDEDQVARIQDEEGWELVPLKGKVIGIKTSILDDKHMAIELIVIYAQVLEDAFEPYVNDIMDKIALPGLAFFFHDPVRVASAKCVPALLNAYKKAHGPESTQLGQLWERTVERVLEVLSTEPAIDTLAEMYQCFYECLECIGRNCLTSTHMSTFIESARSVLQDYQERVKERLEEQAENEDGEEASEETLFAIEDDQNLLSDMNKAFHTIFKNMGTAFLPHWELLMEFYKMAVVNRDPTQRQWAICIFDDVLEFCGPQSWKYNEHIIQPLIDGMQDDVPANRQAAVYGVGVAAHKGGEAWSEFAAASLPMLFQVIQRPNSRADDDVFATENASAAIAKILHYNAAKVQNWQEVAAAWIDTLPVVNDEEATPYAYAFLTQLVEQQNEAVFSNPAKVFTFVVQALEAETLQGQTASRVVGAVKALIQATGTNLAQVAAGLTPEQQRTAQAYFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJU4</accession>
    <name>A0A9W9CJU4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="11">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">DBP4</name>
        <name evidence="16" type="ORF">N0V83_006960</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="11"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJU4"/>
    <dbReference type="OrthoDB" id="10259640at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17941">
        <property type="entry name" value="DEADc_DDX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11 16" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 11 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="15">
        <location>
            <begin position="49"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="80"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="278"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="492"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="694"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="10">
        <location>
            <begin position="49"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="18"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="523"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="694"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="747"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4367378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="808" mass="90915" checksum="E4BF7F97113E85D0" modified="2023-11-08" version="1">MVAPGITGRTRPTKVKKRENAQKRKRDDVDVEKLEQAVQELEPKTSSYKDFTDLPLSDPTKQGLKSCHFAVMTDIQSKAIPLALQGHDILGAAKTGSGKTLSFIIPVLENLYRLQHIGPDAGLGALILSPTRELAIQIFDVLRKIGRHGHMFAAGLLIGGKSLEDERQALTKMNILVATPGRMLQHLSQTAAFSVDDLKMLVLDEADRILDMGFQRDVDAIIEYLPKERQTLLFSATQSRKVSDLARLSLQDPEYVSVHAEDKSATPKGLTQNYIVCPLDEKLDTLWSFIQASKKSKILCFFSSAKTVRFVYESFRHMQPGIPLLHIHGRQKQGARLDTTAKFSSAKHSCLFATDVAARGLDFPAVDFVIQVDCPDDVDTYIHRVGRTARYNREGRGVLFLAPSEEEGMLKRLEAKKVPVELINVRQKKRQSIKDQLQNMCFQDPALKYLGQKAFVTHVKSIYLQKDKEVFKLKEYDLEAYASSLGLPGTPRIKFMKGDDSKQKKQASRQTIEVSDSSEAEQETEKKAKPVRTKYDRMFERQNQDVLADHYKKLVRDGSGDGPSVADDFIGEATANDGHDDDFMAVKRRIPANDEDEDFGADDDTAVAPGGRVVHLAGASQPLVIDSNRREKLLKSKKKLLAFMDKGKKLIYDDEGNPHEIYELETEEQFKAKGLPEEQRQRFIEAATEVVKAADVEDKAAAKAKRQEKLRKRKERERNETTEGGEQGVELEDAGEDLLANFRADAEFTDEDEEADEQPKKQKKEKKWFQSDSEDDERSKKRKKKAKHVVEEPETLEDMEALAAGLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKB0</accession>
    <name>A0A9W9CKB0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNase MRP subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">RMP1</name>
        <name evidence="2" type="ORF">N0V83_007541</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKB0"/>
    <dbReference type="OrthoDB" id="5414547at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000294">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, RNase MRP-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22573">
        <property type="entry name" value="RMP1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047205">
        <property type="entry name" value="RMP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047204">
        <property type="entry name" value="RMP1_RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37792">
        <property type="entry name" value="RIBONUCLEASE MRP PROTEIN SUBUNIT RMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37792:SF1">
        <property type="entry name" value="RIBONUCLEASE MRP PROTEIN SUBUNIT RMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20945">
        <property type="entry name" value="RMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase MRP protein 1 RNA binding" evidence="1">
        <location>
            <begin position="30"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="172" mass="19395" checksum="CB535B6D1BD077C7" modified="2023-11-08" version="1">MPSASPAITATTLLAASQHEKAMLHDIYELLDKLFARNRNQHRRSHWWKSLHAFRKQTGLLLQEMESKKSEREAKLEARLRYWDEGCIHQWYYQFSQLVAVGPFAMLGLVMMASVARICRISGITAVYEEIASGDVQGVLSANGELALAAGFSGVLDAEEEWDEGVVVQREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKS8</accession>
    <name>A0A9W9CKS8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_007174</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKS8"/>
    <dbReference type="OrthoDB" id="5375558at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053175">
        <property type="entry name" value="DHMBA_Reg_Transcription_Factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38791:SF13">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38791">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="153"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="192"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="16"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="203"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="222"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4367589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="596" mass="64968" checksum="CEC1E6BD2F551047" modified="2023-11-08" version="1">MADPNVQQSRQQAVAYPSPHSYPSPSMQPTYTYPPPQGQQSNEPYRGSPQGSNMSLPPLNLPPIRLQDGQQPPPQPQPPQQPMGSPLPPPQHGMPQYYPHPAHAQPGQPMNMGNMGPAQFNAMRYQLPPQGADQRVLSGGRHKKEIKRRTKTGCLTCRKRRIKCDEAHPMCRNCQKSKRECLGYDPIFKQQSGPAQIQPAPNSAPAQHSTTPAPASAPPAPATYSQSPVPQGYAPASSAGFAPAASATSAGHQPENFNAIDPALAQSGSGMHSQPPPYNGVHAMDPAMRAPPGANYPPAPPPEPVKGKRLNIADIYGICNHSPPEVPQRSAPVPPGLDEEFTQIFVNDYCQGLDVMLQTTWFSSNNNALNRVFADRTLHEEAAFFTETIKYKSSNTDMTGVFSQEARLIWHLLGSCKHTPPITNGTNGPANGFAESEDLALREVRARFDVLEALLTNQNMESNPLRQLSYPADIQDTKRAEVDFWQSLGDFAQYSDSETAPPGAADHALATMRNVLHMQEVRDAIYSIAIARHYGSRLSGFPNALPSAVNQHAESDLNKLLVAMSFISHESRSATQQVLARICDMAMLSWTVSRTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL66</accession>
    <name>A0A9W9CL66_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fungal-specific transcription factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006637</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL66"/>
    <dbReference type="OrthoDB" id="5130013at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF39">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="38926" checksum="A4EAC412B635ADE5" modified="2023-11-08" version="1">MGDALMRMALSSGTPSSAAVLKSLLALSSLHRSGLHVQSINLKIESLGALRVASKSELGVKELIQHVAAGMLLCSFEVQQSSCTSSQWMCYITGVKGVLSSTRLRPCHQDSDFNALKQWVYYHDVLARFTMRHWDPAAYTFCVMMRSNMTQFMSSECIPSSQSKPSILTILKLLSEVCQTVASRPQATKSGQQSDDYKSYLEVLDWRVRNIPIPSKDDETPAMVAIIELYKLAISVYLYRATENLLDQSVRTQKQIERGFTLFSQLDSCERQFPVLILGCEARTDDQRAIILDLIARTECKMSSRSFNHVSLVLAALWAQDDLADGELNYWDKLTSIISSCEILPSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLC9</accession>
    <name>A0A9W9CLC9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LSM2-LSM8 complex subunit LSM8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LSM8</name>
        <name evidence="11" type="ORF">N0V83_006833</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">LSm subunits form a heteromer with a doughnut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLC9"/>
    <dbReference type="OrthoDB" id="422364at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01727">
        <property type="entry name" value="LSm8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000027">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034103">
        <property type="entry name" value="Lsm8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044642">
        <property type="entry name" value="PTHR15588"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588">
        <property type="entry name" value="LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588:SF9">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4368476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="98" mass="10965" checksum="73D911A382824513" modified="2023-11-08" version="1">MALNVYLNKRVSVLTNDGRTMVGLLHSCDHSMNLVLQEAVERIIRPRDEDEPSEEVPLGLYIIRGDSVAVVGRVDEEIDSKIDWTKVHGEVLGSTKHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLD6</accession>
    <name>A0A9W9CLD6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006156</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLD6"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4369073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="152" mass="16509" checksum="86DEFE63B43F5BCF" modified="2023-11-08" version="1">MAATPTSAIGTPRPMPIFWRRVSPELLESGASGSVASLEVGAELAVEVLDREVEEACEVDDVDNVVDDSAVELEELLLVDTLLDSDSVVLLDIVSELDESVCDDCHELVSDAGTEVAALDIPRQFQEPFWLVIIQELFPKNAGDGWHVCRSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM25</accession>
    <name>A0A9W9CM25_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_005478</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM25"/>
    <dbReference type="OrthoDB" id="425534at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013595">
        <property type="entry name" value="Pept_S33_TAP-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051601">
        <property type="entry name" value="Serine_prot/Carboxylest_S33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248">
        <property type="entry name" value="2-SUCCINYL-6-HYDROXY-2,4-CYCLOHEXADIENE-1-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248:SF30">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08386">
        <property type="entry name" value="Abhydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040990158" description="AB hydrolase-1 domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="89"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S33 tripeptidyl aminopeptidase-like C-terminal" evidence="5">
        <location>
            <begin position="397"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4369715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="53223" checksum="C1F639C1E2F22399" modified="2023-11-08" version="1">MSSGRTMASYILCIALVAVPAFATPISRHTPRSGISWGNCTVWPSLPANLQCANLSVPVNWDAPDGDHFNLGMVRLPRPSNSTAERVGHLFINPGGPGGSAVQTVASIAGGLRNVSSSLLNSFDLVGVDPRGVGHSAVSQCDPAIWNERVSQFPKTKEDYDRLIDHNKRLGESCLNRTGDVINYLDTISAVKDHEAVRAALGEKMNWLGLSYGTQLGSQYAQLFPDNIRVMVLDGMLQHSQTESSNILIESTAYAAELEAFFTWAGTNESSPLKGQDVEKLWYTILQNATDTPIPAPDCNNTTCRQDVNEEEIRLNAQGLLLRTSTRATFAEALLQASEGNASLLSTPLASDSDPSLFAGITIGCQDWSSYASSFEDFQAKERIGEVYAPLNKGACQSWTLQASCVGWPSPIKNPPAKLNVQTQKPILMVNAIRDPSTSYTWAVGMLEEVKNNVLLTRNGDGHTSWGLGGATTDAINHFLISGELPAPGTVLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMA8</accession>
    <name>A0A9W9CMA8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005719</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMA8"/>
    <dbReference type="OrthoDB" id="3786824at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="595"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="394"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="413"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="476"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="526"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="538"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="604"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="644"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="749" mass="80822" checksum="44834F70813A2CAD" modified="2023-11-08" version="1">MAPSSNTPYNPSVNSAPFMVLPDHVVGFIDLNTLADIHRYLSESEAEEDVALADWVKEKYLHEPINESGRTIRTTEGFQKLDRAYRRVRSLTNKAQDKWPFFDRNWKQMSSATVRNNARGEAAPGVSLPSMYGDQRASSATTGAETTKDSPAPRLQVPFGTKQPTRRSVLGKHDPRPSTSKKHQKTEEQAQFFSLPESSDLPRSEPEPEREPELGHQEPTETASGLLNNEDHLSESIAQAILQSTVAPKEEPEVLRAASDASALDPMHAAASSAQPELTTGLEVEVPRDAGGGKVRGPNGRYLPKDKPSPISSKSGRNSSGIQKKKASAKPTGSRMSGRNTQAFVDHLNNSTEVLPLSVPTQGGEPVEVQSANSGASPQLEAQPLPLSPENDGLVDRAASEPRSHIHLNDIISSRSTSLGSTHSGSLAAESEPVFSNLDRLPPHCARRANKRKSDSPKQQEPRKRARHSGPVGRSRKSERQASKSDPDAVKDTEEKDLSLSQDTAEEEVPQMVTRRITRRSAASTLGSSTPTSAGPKQRQDTVETKDVAMKAAIVGGIDGEDTTTGHEASAPAPTTYALKPSLVAKLKVGRVSAAVEDKPKSAASATMPESQLSDTDLLAIGISKTSSPTRRSSRKKLSTPKSAKTQQPVKSSDTPNGSASAPSAHTANGTATEESATGISPPRPRSRVEYFARVNTLANKVEVPVATEKIDSDENEVIEKYAEWMTQERNMDLSFEQFRSVFGFAKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CME7</accession>
    <name>A0A9W9CME7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_004881</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CME7"/>
    <dbReference type="OrthoDB" id="6339427at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005366">
        <property type="term" value="F:myo-inositol:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904679">
        <property type="term" value="P:myo-inositol import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF22">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="53"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4370363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="430" mass="46247" checksum="2788A155472A5DEB" modified="2023-11-08" version="1">MAYDSKNEKKVDLGRTTSIDRIEDISNAREYADQIAELSGIEATAASKAAWLISITVSLGGLLFGYDTGYISSVLVTIGKSLGHELTSSEQELVTSLTSGGALIGAVIAGLLADKYGRKLPIYLACLVFVIGTVLQTAAYHLPQFAVGRFVVGLGVGSAAMVVPLYISELAPAKYRGRMIAFNNMSVTFGQFVASAIGAGFAQVGGSSSQAWRATVGIGAAPAIILAGLLTLCPESPRQLVAHGKADEASVVLTRIYPTSTEEQRRAKIMSIEMSIQEVTQTMVDDSLWQTLARIFTTPSTFRAVFTACTIMAISQLSGFNTLMYYSATLFKIVGFTNATAVAITVSATNFVFSVVNLVIVDKFGRRRIQRYFESIVSFVQICILTDKYSPDLDTLSTCPLHHSSPHTQMLNAASVSPLSRLLFRHIDRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN15</accession>
    <name>A0A9W9CN15_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ADP-ribosylation factor GTPase-activating protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_005292</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">GTPase-activating protein for the ADP ribosylation factor family.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN15"/>
    <dbReference type="OrthoDB" id="10266696at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08204">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07608">
        <property type="entry name" value="BAR_ArfGAP_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000051">
        <property type="entry name" value="ARF GTPase activator (Csx2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000252">
        <property type="entry name" value="ARF GTPase activator (Csx2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000017">
        <property type="entry name" value="ARF GTPase activator (Csx2), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045258">
        <property type="entry name" value="ACAP1/2/3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180:SF160">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN EFFECTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180">
        <property type="entry name" value="CENTAURIN/ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="659"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="8">
        <location>
            <begin position="836"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="538"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="769"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="796"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1015"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1080"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="256"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="538"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="565"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="796"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1015"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1115"/>
            <end position="1124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4370770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1149" mass="127147" checksum="DD4EE0031DD39BBE" modified="2023-11-08" version="1">MGAVNSKAGEAGPLYLRDQTRFSVASLNIVNARNRTLLNITPNAFPATRYGARREFGDDSVVEYIQDPEAPAPNDGPPSFLIRLPNDEELTFNFTFILRQHLAVPSIYNVNSNSTIAPSGLVDTCINGLTFVFASSSKELDNLVTREFHANPNLHKNPQVELVGDYSTGGSSSVQFQWSWKWTPPSTSESRGGGWRTSCSFVEYDQRAHKLDTLASFTFWVQNTTRLQSPHSPSPRMELSVPPRLRVPSAQSMDSRVSDTTDDYKEWDPVPPKSPIFEAIPENGLSQIPSQATTLTSGSLPMRLDLSRPGEDLSQTDDGPLFRATMKSLEQKTGNMRTRWKKVLKRAESAMEAQVQCNNAMSDLMDALKEASSSNANAVQPAIDHYFDKIAKEILAYERSNTTNIQKLIIDPIYKLYNVDIKQAETKRKDFEEESKDYYQYLGRYLGQRQDSLKEKKRAETDSKYQAKRRNFELKRFDYSSFMQDLHGGRKDQEVLSHLTRYADAQAKYYLETAKKIETMLPQLEALSSEVKEADKEFQIQRTEREEKRRALEKTKKTFDEPPSTAMSSVSALSGSAPRVPTKTEPELPGRKSSIAPVFQSTISPPQSSMSIPSVASPQSPEISKANLATITPQPDKFKGIRDLEEKDLAALVDADLGTHRKEGLLWALSRPGSHVDPKGLNKTAWHKFWIVLDQGKLSEYTNWKQSLELHMEPIDLRMASVREARGADRRFCFEVITPQYTRVYQATNDDDMKSWINAVNNALQTAVETVGKSDRPSTDSLPGSKTRDIASVLTGKSPSLSSHRNQYASKPPARHATVGDRPGGYRREDALGDEGKLLRQVREADASNKVCADCGSEIKVDWVSINLGIIICIECSGIHRSLGTHITKVRSLTLDVTSFTPDIVEILLKIGNRISNSIWEARLDQSLKPGPMSTREQRLHFITSKYSDRAFVAPISPAISHYATPEETLLASVKKNDISNVLYALALHANPNARDRSRGTHAVFLALAAADPASPSASASPAGSPGPGARPSTAQPSRKPFSVAELLLQNGAELPTLPAPIPLSHSARLYLDFKTDQRAGKRVPPVVGSQPPPSSDTLTALPSIIAGNGSSPAERAREREARLQKRVSAGGRLVKTPNFDISDGKRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN64</accession>
    <name>A0A9W9CN64_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004633</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN64"/>
    <dbReference type="OrthoDB" id="5423360at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46310">
        <property type="entry name" value="AMIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46310:SF7">
        <property type="entry name" value="AMIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040916749" description="Amidase domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="Amidase" evidence="2">
        <location>
            <begin position="174"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="613" mass="65039" checksum="BB5849722F05951F" modified="2023-11-08" version="1">MAFIRALGIASLLASGVRAAVSSTGFTVSLTDIDYFLPPKPVASIAGCDEIQSLFDGGLFVPFTVVKGDGITASYSEDDVWQESFLDALYVQGSSKPANISGTILSGTASSELMPGPYFINAAGQVYEAWRLYSDVAGAFLESVMSNGDDSYSVLPAGTVGQKLAIAVPSRLYFEKSAEKPLAGVRIGVKDIYDLKGLRTSCGNRAYYWLYPPASNTATPVQNLIDAGAVIIGKMLTSQFANGETATADWVDYHEAFNPRGDGYQDTSSSSAGAGAGTASYPWLDVSLGSDTGGSIRGPSQVQGLYGNRPSHGLVGLEHTMPLSPVLDTAGLLARNPTLWKEAAQAMYGANITITDTYPKSIKTLAWPTSPSNVANQLLIDFLANVTDFLGANTTAYNVTASFGSDNPELPSLTTLMNLTYALLITKQQIGLVRDPFYADYAVKHDGRLPFVNPVPLARWGWGANQTNTVEEAIANKTAFMSWANTTFLAPSPQTCSESLVLYVGSVGGTVYRNTYWTAPGVPFGFGNSRISVMAEVPDFVVPIGEAPYNSTITGHVEYLPVTVDFLAAKGCDGMLFSLIGALYEAGIVKESLTGQSGITGGEILLRRGMAEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN79</accession>
    <name>A0A9W9CN79_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_004484</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN79"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000297">
        <property type="term" value="F:spermine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF182">
        <property type="entry name" value="POLYAMINE TRANSPORTER, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="316"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="53"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4371267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="373" mass="40353" checksum="164AA669B00D4E90" modified="2023-11-08" version="1">MSSKNSADIEVQARASLDSKEICEQTKTLTDDWDAPDNKENPRNWTALKKACHIIPVALLCLSVTAGSSMITPGVFAISHKFQVSHTAALLPLSLFVLGLAIGPVLAAPISETLGRGIVYKVSMPLYMLFILGAGFSNSFGGLLVCRTLAAISGAPCLAVGAGTVADLFEPRRMAAPGALVVMAPFLGPCLGPVIGGFSATYTEYRWTQWSTIFLALAAYILVLPTHETHRNVLLKRRAKQLGLPPPEPELAPREAIKLLLTITLFRPIRMLVSEPIVLLYSVYNAFTFSVLFAFFEAYPFVFMGKYGFSIWQYGLTFLGIGVGVLLGALGAVLVDLLVYQKIVRKGGDRITKKGPEQRLYIGMIGDLCLPIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN86</accession>
    <name>A0A9W9CN86_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase eIF4A</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Eukaryotic initiation factor 4A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Translation initiation factor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">TIF1</name>
        <name evidence="23" type="ORF">N0V83_004140</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the eIF4F complex, which composition varies with external and internal environmental conditions. It is composed of at least eIF4A, eIF4E and eIF4G.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. eIF4A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN86"/>
    <dbReference type="OrthoDB" id="206213at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18046">
        <property type="entry name" value="DEADc_EIF4AII_EIF4AI_DDX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03426">
        <property type="entry name" value="NUDIX_CoAse_Nudt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000089">
        <property type="entry name" value="Eukaryotic initiation factor 4A-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045121">
        <property type="entry name" value="CoAse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044728">
        <property type="entry name" value="EIF4A_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 17" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 23" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="21">
        <location>
            <begin position="23"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="54"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="235"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Nudix hydrolase" evidence="22">
        <location>
            <begin position="492"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="595"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="644"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="687"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="23"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="595"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="693"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ4372366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="800" mass="89509" checksum="EAED0D8C2732CFBD" modified="2023-11-08" version="1">MADKGLEDVPEGQIESNYDETTDSFDSMNLKAELLRGVYAYGFERPSAIQQRAIMPVIKGHDVIAQAQSGTGKTATFSISTLQKIDSNIKACQALILAPTRELAQQIQKVVVAIGDFMDIQCHACIGGTSVRDDMKALQDGPQVVVGTPGRVQDMIQRRVLKTDHMKMFVLDEADEMLSRGFTEQIYDIFQLLPQSTQVVLLSATMPQDVLEVTTKFMRDPVRILVKKDELTLEGIKQFYIAVEKEDWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLTARDFTVSAMHGDMDQAQRDVIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADDVRMMREIEQFYSTQIEEMPMNVAGKHLLNLDAATRFPIIWLSIASAHDINPSDSPSPVARTTHEANSMARSKKSHCDHGAFPIICCRFAPSLPVVLEVAGQALNRLRTYTPPPTQWYNCPLTRRAAVLILLFPDRHGELKVVLTMRSAKLRSYAGQAALPGGKADTLEESPWDTARREAYEEIGLPMNEEKLRGFKVEHLCELPANLAKTELGVRPCVAFLCPSNTSSTTSSSPTSTSSTTNGTEEPSVEERLIPRLDPKEVAAVFTAPFPSFLRKEWSGEGPPPVQKNGKPEKWYRGSWTDWHESRWRMHNFYIPRPPPTPSTLLRNPSNSSSSGPEPSPHQALPEGDDPQPEPSALDELTTFRVFGMTARIMVDAARVAYGEEPQFEHNSHFGDEEMIGQLLRMGRLSEVRREGEVLTKEVIREASKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNL5</accession>
    <name>A0A9W9CNL5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 13">Ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="8 13">5.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">RPE1</name>
        <name evidence="17" type="ORF">N0V83_003917</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="15">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate degradation; pentose phosphate pathway; D-xylulose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 13">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="8 13"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNL5"/>
    <dbReference type="OrthoDB" id="1927044at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004750">
        <property type="term" value="F:D-ribulose-phosphate 3-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000130">
        <property type="entry name" value="Ribulose-phosphate 3-epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02227">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026019">
        <property type="entry name" value="Ribul_P_3_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004076">
        <property type="entry name" value="PRK05581.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="RIBULOSE-5-PHOSPHATE-3-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001461">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01085">
        <property type="entry name" value="RIBUL_P_3_EPIMER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="12 15" id="KW-0170">Cobalt</keyword>
    <keyword evidence="11 13 17" id="KW-0413">Isomerase</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="10 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="174"/>
            <end position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="225"/>
            <end position="226"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00009541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00013920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR001461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4372144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="252" mass="27017" checksum="184B84FBBC7DB6EF" modified="2023-11-08" version="1">MSPPAIIAPSILSADFGALGRACSDTIEQGADWLHVDIMDGHFVPNMTFGAPVVTKIRSHVDRPATAHGKGTFDCHMMIAEPKKWVRDFKKAGCDLYCFHYEAAVSSTAADSPSATSDRKTSPKELIRFIHNEGMQAGIAIKPGTPVHVLWDILANPVKQEVPDMVLVMTVEPGFGGQSFMASELPKVSALRQRYPELHIEVDGGLSEKTIDQAADAGANVIVAGSAVFGAADPGEVIARLREAVESRRGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPY5</accession>
    <name>A0A9W9CPY5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002768</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPY5"/>
    <dbReference type="OrthoDB" id="5361617at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="394"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="415"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="475" mass="52681" checksum="DF3A25C4F17CBB26" modified="2023-11-08" version="1">MRRSTFPPRSSKPADTMVRVPEQPKRKVFNCIVDDTALVAGVKKSTRDGIRKWISQGAIRLFVPLHSMRPCTMIDVSPLTILLALTQLHRLKNGDERVNADAREAVKWLDEVTSIPAVEVANRVQLEGVEEAYTTWAEVAQFLLPQTLLSMEESDSEEEDYHEDLESSFNALDLSDETSMSSTHSFDETPRTPESPKSCLSKDLKPTNGAMQETQSEGVIVQSPDRTARNSTEMARAEKTPKGAVPVHLRPLFNHILWRINKEENPDAALESFILLTNDPTKQAIAQKFGIRAKRLEQLRDAVAREDREFKNHLTMHKLEVESTASKEGPASPSKAVNRPASSHTSATAPPEENSDDEDVVLFQRAPRGPAATTNNQRVFDPNDFGRVNQIAQHRPQRGGRGGPIAPRGRGGPPFRGRGTFAPRGAYVPPGPVFRAPPTPRHDPNQPIDPDSFTRPVPKAGPVRGGGRRKLWEPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQB0</accession>
    <name>A0A9W9CQB0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001291</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQB0"/>
    <dbReference type="OrthoDB" id="415532at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025340">
        <property type="entry name" value="DUF4246"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049192">
        <property type="entry name" value="DUF4246_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049207">
        <property type="entry name" value="DUF4246_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33119:SF1">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33119">
        <property type="entry name" value="IFI3P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14033">
        <property type="entry name" value="DUF4246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21666">
        <property type="entry name" value="DUF4246_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4246" evidence="3">
        <location>
            <begin position="33"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4246" evidence="2">
        <location>
            <begin position="151"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4376011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="709" mass="80341" checksum="2A0EBA631832CF54" modified="2023-11-08" version="1">MARTSQIRKQQASTPASQAQPSKLIQLPKLLEYPGLGLPLRHNDQNFKRGFYPLGAHGSCSGSHSDLIPVRELAMMDVMEKLTDKEDWHKKVFDDEIVAKWRKEALAIPDEEFWKLAINGKRQWWDSNDDVRLQIDSSGGVEMSKGIMSEAAFSCCIEELRSKARYFEKTDIIPTLDASASVAKSDQIVSPELHESLRKAFDTLKVDHEVSPDWHPNSNDMVQDLVHPSMYPLVYGRSRGFKEECIGVVEAIEHWAGKGDLIPKDDWVPDANDRYRYGVGSGTVPPNYWSETYQWLPANVAFQEDGSVKFTSYINNLHPTKYPEIYRTTEDLVKTSLPLWDQCLTMATGYQKRDGAGRTEPRFGKPDNPDDENPDNWTPDMEDCAHAEVSEKDLSDRGYDPKYCGEEDLTRYKWEVVRKARLPEPTFNDIDYAREEGKRLVDRFRDSGLQIIVKMASIELTPEKPDFPGGGWHVEGQMNEHICATALYYLDSENITNSSLSFRMQTSAYISDDEGYNVGQDAYHWMEEVYGTTLGSGGSPCLQNYGTVETRQGRLLAFPNVLQHRVSPFQLIDPTKPGHRRFIALWLVDPTKRIISTANIPPQQMTWYVDSLLGSTSSARDEALSKLPPELVALLEQHAVTGAESTGNAKLPAELMIMVKEYFEAGKEALPMTFEEAREHRAKLMAERGAFVKTAEGGWQQHSYSFCEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQK0</accession>
    <name>A0A9W9CQK0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="13">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">CBS2</name>
        <name evidence="15" type="ORF">N0V83_002163</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 13">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12 13">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 13">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 13">Belongs to the SHMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="13"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQK0"/>
    <dbReference type="OrthoDB" id="10265628at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000097">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF57">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 13" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9 12 13" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="14">
        <location>
            <begin position="53"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4375083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="524" mass="57960" checksum="18EE17F85F11749A" modified="2023-11-08" version="1">MATLRAVCRAPLRQLSARSSLSIATNHARILPQQWRGYATTLEAQQKVLSQDLEQADPTVYQIIEREKNRQKHFINLIPSENFTSQAVLDALGSVMQNKYSEGYPGARYYGGNEFIDEAERLCQERALKAFGLSSSEWGVNVQPLSGSPANLYAYSAVLNTHDRILSLDLPHGGHLSHGYQTPTKKISAVSKYYETLPYRLDEKTGLIDYEKMAELAHLYRPKVIVAGTSAYSRLIEYERMRKLADEVGAYLLSDMAHISGLVAAGVIPSPFPYSDIVTTTTHKSLRGPRGAMIFYRKGTRKVDKKGNEEKYDLEGPINASVFPGHQGGPHNHTITALAVALQQAQSKEFKEYQQQVLDNAKALAQRLGDTKENGGLGYNIVSGGTDNHLVLVDLKDRGVDGARVERILELVGVASNKNTVPGDKSAMKPGGLRIGTPAMTTRGFQTEDFKRVADVVHRAVGITQKLDKEAKKKAEENNRKNPGSVAAFKEYVGEGEDITDIVQLRKEVEDWVSTFALPWDKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQW2</accession>
    <name>A0A9W9CQW2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_000141</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQW2"/>
    <dbReference type="OrthoDB" id="419598at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05259">
        <property type="entry name" value="PCBER_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051609">
        <property type="entry name" value="NmrA/Isoflavone_reductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045312">
        <property type="entry name" value="PCBER-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47706:SF7">
        <property type="entry name" value="CIPA-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G01630)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47706">
        <property type="entry name" value="NMRA-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="3">
        <location>
            <begin position="23"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4377316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="323" mass="35017" checksum="B2A7DBD323646006" modified="2023-11-08" version="1">MDNIFEVSFSDTLSIMSAKQGRNIAMVGASGNIGSRTLAAILETGVHTVTAISRGESNATFPAGVAVKKGDYADESFLVNALQKQDVLVIQLGFMVTGQQIPLIHAAAKAGVKYILPTEFGSDPYAPFATVFPMLAEKKKYRDLVEELGMSWIAVVNNPWFDWSLKQGNWGINVKEKKAVLYKGAEAKFNTTTLKQVGEGVAALLSLPDAQLETFKNKPVYLSSFETTQKEMLDSVFRATGGKEGDWNIVVRDATDVIAEGREEVAKGNPFAFVYEFYIAHMQDGFGGDYHEKAAKDAEILGLKAGNLDDAVKAVVQEVATGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR78</accession>
    <name>A0A9W9CR78_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hydroxymethylglutaryl-CoA synthase</fullName>
            <shortName evidence="5">HMG-CoA synthase</shortName>
            <ecNumber evidence="5">2.3.3.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">3-hydroxy-3-methylglutaryl coenzyme A synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ERG13</name>
        <name evidence="8" type="ORF">N0V83_000474</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form HMG-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-3-hydroxy-3-methylglutaryl-CoA + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10188"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.10"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the thiolase-like superfamily. HMG-CoA synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.10" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR78"/>
    <dbReference type="OrthoDB" id="1269963at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00827">
        <property type="entry name" value="init_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000008">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000590">
        <property type="entry name" value="HMG_CoA_synt_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013746">
        <property type="entry name" value="HMG_CoA_synt_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013528">
        <property type="entry name" value="HMG_CoA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010122">
        <property type="entry name" value="HMG_CoA_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01833">
        <property type="entry name" value="HMG-CoA-S_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323:SF2">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08540">
        <property type="entry name" value="HMG_CoA_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01154">
        <property type="entry name" value="HMG_CoA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01226">
        <property type="entry name" value="HMG_COA_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase N-terminal" evidence="6">
        <location>
            <begin position="4"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase C-terminal" evidence="7">
        <location>
            <begin position="178"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="3">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="460" mass="50809" checksum="400CCAA63B5279C7" modified="2023-11-08" version="1">MSTRPSNIGIKGIELYIPSQCVDQAELEKFDGVSQGKYTIGLGQTKMSFCDDREDIYSLSLTAVSSLFKKYNIDPKSIGRLEVGTETLLDKSKSVKSVLMQLFEESGNYNVEGVDTVNACYGGTNALFNSVNWIESSAWDGRDAVVVAGDIALYKKGNARPTGGAGCVAMLIGPDAPLVMEPGQRGTYIRHAYDFYKPDLTSEYPIVDGHFSIKCYTEAVDACYKAYNAREATLKSQQNGNGVNGHSQELDTPLDRFDYMAFHAPTCKLVAKSYARLLYNDYLVNPSNPIFAEVPAELRDMDYATSVSDKTVEKTFMALAKKRTAARVQPSIQVPTQCGNMYCGSVYGSLCSLLANIKSEDLQGKRIGMFSYGSGLASSLFSFKVKGNTESLAKQLDIHNRLESRRVVAPEVYDEMCNLREKAHLKKDYTPTGAVDTIFPGTYYLTGIDGMFRRTYEIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRG6</accession>
    <name>A0A9W9CRG6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001672</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRG6"/>
    <dbReference type="OrthoDB" id="2336090at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF19">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="148"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="393"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="287"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="531"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="660"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="710"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="940"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="317"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="531"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="549"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="676"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="732"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="946"/>
            <end position="961"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4376389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="986" mass="107808" checksum="2E7C39B2D7C3483D" modified="2023-11-08" version="1">MPVNTLSVGAAVTPIVIQTYISHYLNRRPLHQKPTAHISYHEGLELIRRFLHYASFHTVDELQAFTSQWVPVPRWVHVKEVEITNAQLLRSAECINAQLGPEGIKAVGGEKWWQWRREGTTLKAEWIEMRKDYEARKQLNITKGDRIMLYVHGGAYFFGSVDEHRYQMQRHARKLKARVLAPKYRLAPQFPFPCGLYDCLAAYLYLLDEKHDPTTIILAGDSAGGGMVLSMLVTLRDQGIPLPAGAILISPWVDLTHSFPSLGGDGKMDYIPAHGFIHKPSMSWPPPNADDMLELERSSSSQKQKERKSPRTSMSKGHKEERAAQKEAKAERVRGYSVRSDQPDVKSLLDLSGQSQSQEADTWISPDGKYSIGPKSTLSVQLDNDVRIEIKDQIQMYAPNHLLTHPLVSPALQPTLGGLPPLLIQTGGGELLRDEQIYIAHKAAQPLAYLPPPSNNQTAESIQAQAARYRPTNVQLQVWDDLCHVCPTLSFTRPAKYMYRSIAQFGAWALARAQKKSIDILDDDDISFVSTDSSASSSSDKPGSNSSLEDAKASKNRDVPQDRTTKAVKQSGTTVGRAGDALPPFERHMIRQRIDRHGTIYPLSAQEELVALNLATSEIGVPKTGPVSKWMKAQQQWNSKFSKQKINIQKQRIKDMQKGFEGFGGEKPPPTALAGRRVKGMKAEKAKKKSWGMAMWSGWGSKHDEATITREEKADHTQDQAQPSKSDMEPAQNKTDGAVETQTNTKAKRTRGSTLTAPDKLRSRSHSRHSAVTDKGQTGASVEDFPQIAPPAPGLAEATVLAPERRASPLPSPTSELSSPGFPPPGIIVSDNPQSPHTLIPATDTLTTRPTKGGVAYPFSLKVDGPDGKDFNASTATLESVNIMTPAAVDVPQHEKELGAGVAGEAAVIGTGSVNEQVLAEERPGVERYFTAGPGVGLFSSGDGVQEEKAAVDGEKGARPPVERFETAMEDLSTLADAGAGAGAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRL3</accession>
    <name>A0A9W9CRL3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">V-type proton ATPase catalytic subunit A</fullName>
            <ecNumber evidence="2">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">VMA1</name>
        <name evidence="20" type="ORF">N0V83_000021</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Vacuole membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRL3"/>
    <dbReference type="OrthoDB" id="1676488at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18111">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18119">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01134">
        <property type="entry name" value="V_A-ATPase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000008">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000052">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000003">
        <property type="entry name" value="Vacuolar ATP synthase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00309">
        <property type="entry name" value="ATP_synth_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031686">
        <property type="entry name" value="ATP-synth_a_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005725">
        <property type="entry name" value="ATPase_V1-cplx_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003220">
        <property type="entry name" value="PRK04192.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01042">
        <property type="entry name" value="V-ATPase_V1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607:SF1">
        <property type="entry name" value="H(+)-TRANSPORTING TWO-SECTOR ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16886">
        <property type="entry name" value="ATP-synt_ab_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="17">
        <location>
            <begin position="106"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="ATPsynthase alpha/beta subunit barrel-sandwich" evidence="18">
        <location>
            <begin position="210"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="16">
        <location>
            <begin position="315"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="19">
        <location>
            <begin position="548"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="30"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF16886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ4377198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="698" mass="77161" checksum="D2BEFFAA2A5EA485" modified="2023-11-08" version="1">MRLGLRPRLLRTRPSLLDLIQKKNIDQISTSADRIPVLEDDVEPHRVPLPSSPLISPQPQSAAADQPPPPSITISEASDRAEKEITTAKMPPKQEKKQEHEGHGYVYSVSGPVIIAENMIGCAMYELARVGHDNLVGEVIRIEADKATIQVYEETAGVTVGDPVYRSGQPLSVELGPGLMETIYDGIQRPLKGIADAADSIYIPRGINLPALDRKKKWDFTPGDLKVGDHITGGDVFGSVFENSLFSDHKILLPPRARGTITRIADKGSYTVDEKILELDFNGTKTEYSMMHKWPVRVPRPSNEKLSSDQPLIVGQRVLDSLFPSVQGGTVCIPGAFGCGKTVISQSLSKFSNSDIIVYVGCGERGNEMAEVLMDFPELTIEVNGKKEPIMKRTTLIANTSNMPVAAREASIYTGITVAEYFRDQGKDVAMMADSSSRWAEALRELSGRLGEMPADQGFPAYLGAKLASFYERAGRVQTLGSPERYGSVSIVGAVSPPGGDFSDPVTTSTLNIVQVFWGLDKKLAQRKHFPSVNTSISYSKYTTPLDKYYAKHNPDFPRLRDRIKELLTTSDDLDQVVQLVGKSALGDSDKITLDVATLLKEDFLQQNGYSDYDQFCPLWKTEWMMKNMMAFHDEAQKAVAQGHSWVKIRESTSEIQSQLRSMKFELPSDGEEKVGKKASYEELLQKMTEKFASVVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSE7</accession>
    <name>A0A9W9CSE7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000883</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSE7"/>
    <dbReference type="OrthoDB" id="4196148at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4378053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="243" mass="27321" checksum="80DBDB9960CCAA1E" modified="2023-11-08" version="1">MSTPVPSYEEANNFAPPTTINPSSQPPPLPPTELPPAYAVVDEKATNYTIYGTIIHTPSGPAYQLSSLLDACISRLRIRRLRAEEISRLQAGAPNVSFDNTRVLYEAHDPPFAVNEYFVQGKQSGCLPGVLQLRFGLRRWHVRHLPEQGGRANDIMTCGKRGKLGRVIRERRNEMEPSEWKDTDGTVMATEVLKIEQGGKMPTIELREDLDQTCRELILALWVTRLWAAFGAGTRSMYQQEGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZB7</accession>
    <name>A0A9W8XZB7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_010946</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the aldo/keto reductase family. Aldo/keto reductase 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZB7"/>
    <dbReference type="OrthoDB" id="48988at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050523">
        <property type="entry name" value="AKR_Detox_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364">
        <property type="entry name" value="NADH-SPECIFIC METHYLGLYOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364:SF7">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="4">
        <location>
            <begin position="29"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="4">
        <location>
            <begin position="171"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4362005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="411" mass="45897" checksum="96ECE4480B2D370B" modified="2023-11-08" version="1">MTFVAPKVPVSLLGRHRILSRTAGVRVSPLCLGAMNFGTAWTSFLGECSKDTAFEMLDYFYEQGGNFIDTAVNYQFGESEQWLGEWMEQRGRRDEMVIATKFTGMQVLEEEKAGGKAKTVKSNMAGNGAKNMYTSIERSLKALKTSYVDIVSPRFVLGHLRRRQAYTSWQYYCHLWDSTTSIPELMHALNDLVTARKVIYLGISDTPAWIVVKANCYARQHGLRQFSIYQGRWSAAERSFEREIIPMCLDEGMALAPWGALGGGGFRTKKQREEGKEGGRKMAMTKIGNEEKVSDVLEKIADSKSPPTLITSVALAYVMHKSPYVFPIVGGRKVSHLEGNIAALGLRLTPEEIQEIDNAYGFDMGFPHSFLNPANVVLMGPEDNRFTGQCGFFDFVETPKPIPPHEGKLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZK7</accession>
    <name>A0A9W8XZK7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Leukotriene A(4) hydrolase</fullName>
            <shortName evidence="14">LTA-4 hydrolase</shortName>
            <ecNumber evidence="14">3.3.2.10</ecNumber>
            <ecNumber evidence="14">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LAP2_2</name>
        <name evidence="16" type="ORF">N0V83_010300</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>an epoxide + H2O = an ethanediol</text>
            <dbReference type="Rhea" id="RHEA:19037"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32955"/>
            <dbReference type="ChEBI" id="CHEBI:140594"/>
            <dbReference type="EC" id="3.3.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13 14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the peptidase M1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.3.2.10" evidence="14"/>
    <dbReference type="EC" id="3.4.11.-" evidence="14"/>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZK7"/>
    <dbReference type="OrthoDB" id="79562at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09599">
        <property type="entry name" value="M1_LTA4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000009">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.320:FF:000001">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000004">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2010.30:FF:000001">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.320">
        <property type="entry name" value="Peptidase M1, leukotriene A4 hydrolase/aminopeptidase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012777">
        <property type="entry name" value="LTA4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049980">
        <property type="entry name" value="LTA4H_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038502">
        <property type="entry name" value="M1_LTA-4_hydro/amino_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034015">
        <property type="entry name" value="M1_LTA4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015211">
        <property type="entry name" value="Peptidase_M1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02411">
        <property type="entry name" value="leuko_A4_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726:SF3">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09127">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01263">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 14 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 14" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M1 leukotriene A4 hydrolase/aminopeptidase C-terminal" evidence="15">
        <location>
            <begin position="477"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="396"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="142"/>
            <end position="144"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="279"/>
            <end position="284"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="581"/>
            <end position="583"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR612777-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR612777-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR612777-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4363180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="629" mass="71095" checksum="EA34864D2A402451" modified="2023-11-08" version="1">MTDPNVTIRTPRDPNTLSNYHNLVTRHTSVDFNIDFEKKRLYGSVTLTLESLTDEEVKDVVLDSSHLDISVVEVEGKSVKFNVGDRIEPYGSPLTITLPSSVPKGKTVDIEIKVATTDKCTALQWMTPAQTSNKKHPYMFSQCQAIHARSVFPCQDTPDVKSTISFALRSPLPVLASGLPTGASEYQPPNKDGEYGTLKYTFEQKVPITSYLFAVASGDLASASIGPRSTVWSGPEELLDCQRELDGEIEPFMKALESIVSPSYQWTQYNVLILPPSFPYGGMENPVWTYATPSIISGDKQNVDVIAHELSHSWSGNLVSAASWEHFWLNEGWTTYLERRIAAAIHGEPHRHFSAIIGWKALEQSIENFGKDHEYTKLVTDLKGGDPDDAFSSIPYEKGFHALYQFELLLGKEKWDSFIPHYFDTFKNKSLDSYDFKSCLIDFFAKDSEASKKLNDFDWDKLFYAPGYPPKPDFDQSMVTKCYELADKWHSLLTKSTSGDFKPKESDIEGWVSNQSVVFLEKLQSFAEKFSAEHVHQLGATYGYTTTQNIEVLSRYLSIGLMAKAPETYQPSADLLGRIGRMKFVRPMFKKLNAADRDLAVKTFEKNKDFYHPICRQMVEKDLFGDEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y003</accession>
    <name>A0A9W8Y003_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pinin/SDK/MemA protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_009906</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pinin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y003"/>
    <dbReference type="OrthoDB" id="330772at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039853">
        <property type="entry name" value="Pinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006786">
        <property type="entry name" value="Pinin_SDK_MemA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12707:SF0">
        <property type="entry name" value="PININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12707">
        <property type="entry name" value="PINN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04696">
        <property type="entry name" value="Pinin_SDK_memA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Pinin/SDK/MemA protein" evidence="9">
        <location>
            <begin position="66"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="164"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="174"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="226"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="253"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="295"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="315"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4363610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="330" mass="36716" checksum="520686A9C227BEF1" modified="2023-11-08" version="1">MDGAIASAVVLPDDLTGPASPPNGHKRRQSSISEEHAKRPRLNDDDASTEGPQSANGSKPAAPVRREKGRERRLFGAVLGALSQNPTTAAQKRRSEIEKRQFAQRKLQDQESGQRKAERVARRKVQRWKEQKYFEKESMRVRHESLLHMAHFLRTETEPHLYYKPWETSPDEEDRIRDQIAEAHAIIERELDEYEARQRQDNPHQHASADGGGGGRGGDIYDVDGSYNSKNNDGSTPQEPSTTNSGTNGSAKHPKDLDMRDDHAAAEDPGNEAVRDAATTNTTTTEEDVEVDSSRIPSTGHETTFANDPGSKDNNDDELVEEAAEDTVIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y169</accession>
    <name>A0A9W8Y169_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008698</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y169"/>
    <dbReference type="OrthoDB" id="2991872at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4365081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="277" mass="30373" checksum="DD7126448F6BD92F" modified="2023-11-08" version="1">MLEVLNWMFRLPEPDRALLSNHLPDKPSEPSAIPFNSTSGELDNITLRNHHSLTTISIVDMIEKSCSAILCNFTIPIPNKLFDDVVGMRGYVCLWTMGTMDAVLASGLIPDSIVSATESSSSDTQVSLLPRITNTPPQVPATDITSLQNIQLGSQKPFATAVSLPQSSTLRSPQDCYSASMKPSPLALADSNWTTPTGHIFDDGPSHPYDVPVKPPSLDIDIMEPKKLDIAARREWLNCILYYIGTELGVKKALCIPYTENFLSTVKPKVDSIFGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1A1</accession>
    <name>A0A9W8Y1A1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphogluconate dehydrogenase (NADP(+)-dependent, decarboxylating)</fullName>
            <ecNumber evidence="3">1.1.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_009351</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 6-phosphogluconate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.44" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1A1"/>
    <dbReference type="OrthoDB" id="434986at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004616">
        <property type="term" value="F:phosphogluconate dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019521">
        <property type="term" value="P:D-gluconate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000634">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006114">
        <property type="entry name" value="6PGDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006113">
        <property type="entry name" value="6PGDH_Gnd/GntZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006183">
        <property type="entry name" value="Pgluconate_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11811">
        <property type="entry name" value="6-PHOSPHOGLUCONATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00393">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000109">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00076">
        <property type="entry name" value="6PGDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01350">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0311">Gluconate utilization</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="188"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="496"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4363899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="524" mass="57375" checksum="C3E2712286D6AD13" modified="2023-11-08" version="1">MSKFSTIAMIGCGSMGGGMALLFAEDGVHVGLSDPSEEAMDAVVEKAEKAGYNGKVKKHKGMMIDYDSLCKSLSSPRLLVFSLPHGNVGDKVLEGLMPHLSRDDIILDCGNEHFANTERRQEKVKDTGIRYIGCGVSGGYQAARAGPSMCPGGDISALKEVLPLLEKVAAKDKDEKPCVGMIGKGGSGHYVKMIHNGIEHGMMSAISEAWGIMRKMGMGYEEIGNVLSQWNSDGELQGTFLISIGADLSHKREPGKEGKADKNPNHHPLVVSEVLDKVVQDVTGEEGTGIWSNTEAIELHVPAFTLNIAHALRLASAFRGDREKANKACNGGFPPSNLDIQDKKNFIENLRLATYAACLASYIQGFNIIARADQEHKWDIDYSQVWQIWRAGCIIQADYISDEILAPILKSKPSSQDLNLNFSARVAQDIQACFPALRRVVSKAVETDQVVPAISATLEYFKVISGTDLPTAFYEAELDYFGSHMFDVKGDKDSQVKKPMEGKHHFEWKPATSQKEEYGKEYKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1I9</accession>
    <name>A0A9W8Y1I9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nuclear aminoacylation-dependent tRNA export pathway component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CEX1</name>
        <name evidence="4" type="ORF">N0V83_008670</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1I9"/>
    <dbReference type="OrthoDB" id="447103at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF3">
        <property type="entry name" value="N-TERMINAL KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="548"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="593"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="558"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="593"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="612"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="639"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="658"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="745"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="763"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="776"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="786" mass="85319" checksum="2251FDD4A5BB335E" modified="2023-11-08" version="1">MDFLKSAVASAISKGPAFGYTFGDRVDLNDSIWTLHNGTKREDGSKCSIFSFDITANKSRLPLARNALRKLRTLRHPGVVKILDTVETETYIYIATERLSPLSWHVKRKSLTEETTKWGLHNIAKTVKFINADATSIHGCIRPASIFFSESGEWKLGGFDALSSVKEDDSILPTYGSLIPDAGRYMAPEISKAGWEIIKQNPTHAVDAYNYGVLIFEVFNGSYHSSDQLSQMKSIPPSMHQSYKRLLNPNPKSRMSVGQFLDQGKRIGGFFQTPVIQVTDDIENLGLKADDERNELLSKLDEVADDFPADFFKMKVLPELLKSVEFGGGGAKVFSTVMQIGQKLSDDEYETQITPVVVRLFANPDRAIRVCLLDNFPLMIDHLSQKLVNDKIFPQMVTGFTDVAPVVREQTVKAVLTVVPKLSDRVVNGELLRHLAKTANDEQPGIRTNTTICLGKIARNLGASSRAKVLAAAFSRSLRDPFVHARNAALLALAATADLFSEDDCATKLLPIMCPSLVDKEKLIRDQAQKSVDIYIVRIRKFAASMPDTVLPPPNLATGGSATSPRMGTPSNDNSGAGWAGWAISSFTNKLASTSGQMQSNSATNGAADQRSSSVPPTTTSSSKPPVPLASKPGMLLHSSKSAASTPTLTSPDPVTAFDDEAEEFNDDWGGFGDDDSKGQEEEDAWGTPVISKPATTSYDDKGEPDFAGWLAAQSSGKKTTKNPLPKGLAKPSASSSKTTRPVIGGRASTTGSATTRKVVVPPKKEAKKEQPKANEEEEEGWGDAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1Z3</accession>
    <name>A0A9W8Y1Z3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_009106</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1Z3"/>
    <dbReference type="OrthoDB" id="439993at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12291">
        <property type="entry name" value="RRM1_La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002344">
        <property type="entry name" value="Lupus_La"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF140">
        <property type="entry name" value="ACHILLES, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00302">
        <property type="entry name" value="LUPUSLA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="7">
        <location>
            <begin position="113"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="235"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="335"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="40"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="359"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="401"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="462"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4364511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="483" mass="55166" checksum="039BE2D9A309EC5F" modified="2023-11-08" version="1">MADTENAAQSASNPAEAPAPMEVVQSEETTVKPTAAGDDGEVKWDLSKSGDKNDRGNDRDEQRNGDRRDYRGGRGRSQYPSSGFQKKKKSVHIQQWLEAHPDNFDRHNTEFDDLPETDDPEQIRAQVEFYFSSQNLATDEHLFLELEGPKNRPVSLKHVTSFKRMRRFAPYTAIVNALRESKHIDLVDGGDFGQESIKLKEPLKVDAKPGDDEKEPSTLELFNRLKHRSSNDMTASAYVKNFGDEQKADQITLEQFFRPYGAVVVRKRRNDDQSWKGSVFVEFDTEDSQKQFLALDPKPKFNDNELITMSKKEYVEMKCKEKGIKPDWELTEQELAEKRQKQRGQNGNSRGRGGDRSHRGGGRGRGRGGRGGRGGFGGGRDRDRDRSPRRRRDRSASDGSVDERDWKTRRDRFKNGKDDRATRGHSDRKERKDLERDAHGVPVVRDSRTDAEIAAASNKRKARDDEERAESPKKSKIEIKQDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y217</accession>
    <name>A0A9W8Y217_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y217"/>
    <dbReference type="OrthoDB" id="3755201at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="185" mass="20582" checksum="D3D9462C99CC762C" modified="2023-11-08" version="1">MPTDVHGQTHAGSLGLFDDDTEHDNATARAERHEYERLEGVIRRQERRDARQSRKDQKAAEHRAAAAAQGKSKLLPRWLGRLFGGGGGKDLSSDDATPPARPVAVAEGQRGRGIEEERGRSRIPRRNMEEYVIVRRPETPEPVYTGEDVDAVDSPWLRECGMVRVKSDDPGGCHIVPKSVVQKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y294</accession>
    <name>A0A9W8Y294_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1996 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008411</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y294"/>
    <dbReference type="OrthoDB" id="74764at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018535">
        <property type="entry name" value="DUF1996"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43662">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43662:SF2">
        <property type="entry name" value="DUF1996 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09362">
        <property type="entry name" value="DUF1996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1996" evidence="1">
        <location>
            <begin position="5"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="204" mass="21705" checksum="8109F5EF4CD83763" modified="2023-11-08" version="1">MPYSAKNNKMTAFKPGFRMIAGDPMSKTNNRVSICHRCLGNGEGFAPCDNKDTGELPTKYCPKGIRASIIFPSCWDGKNLDSPDHKSHVAYSNSGGLGTSNCPSTHPVAIPQVMYEIMWDTALEMVLGTASTATIFLAGRTTPLQKAMDALPSGKCANANCNVLKVQSAADAEKCKKAQQVVEDVGKDGAWISQLPGGVAVTYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2F3</accession>
    <name>A0A9W8Y2F3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Siderophore iron transporter mirB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_009980</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2F3"/>
    <dbReference type="OrthoDB" id="4078873at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF50">
        <property type="entry name" value="MFS SIDEROCHROME IRON TRANSPORTER MIRB (AFU_ORTHOLOGUE AFUA_3G03640)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4363683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="211" mass="23150" checksum="835041E061119E44" modified="2023-11-08" version="1">MRLSEAGYVNSTFQVVSGVLLFIVGYLIHRTGRFKWLFYVTVPIYTFGLGLMIYFRQPNVKIGYIIMCEIFISIGGSVFILLVQLGVLAAVDHQHVAAVLALLYVTGTVGGAIGNTISGTIWTNTFFKALQQKLPESALPDVLTIYSSLPAQLAYPVGSPERIAIQEAYGYGQARMLAAGIGIFGLSFIWMFLIKNHDVSKKTLQTKGVVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2H3</accession>
    <name>A0A9W8Y2H3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HAD-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009313</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2H3"/>
    <dbReference type="OrthoDB" id="2363873at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051540">
        <property type="entry name" value="S-2-haloacid_dehalogenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43316:SF4">
        <property type="entry name" value="ACID DEHALOGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G05870)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43316">
        <property type="entry name" value="HYDROLASE, HALOACID DELAHOGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="26972" checksum="895502BF615CE33A" modified="2023-11-08" version="1">MTKSACFDVIGTCFSFTHPITTIHTLLSPKLPSTTTLDAKSLFFSWFYAAQRDFTYVSLAGTYTPIATILRSTFRRACLIVDLPTDIVTDSDLDAIMAAFKAMEARDGLKRCFDGLKEAGWGVYGVTNGGSETSLGYFRKAGIEWLGDGELLSCDELGVAKPDVRVYENANEHLTKQGVGKEDGGGERWFVAAHAWDLIAARKAGFKTAYLEFEEHDPVTEVFGKFDLYAKDMDELLEKLKKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2S7</accession>
    <name>A0A9W8Y2S7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009118</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2S7"/>
    <dbReference type="OrthoDB" id="3801226at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4364523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="416" mass="48890" checksum="96B5AB24C0CACC24" modified="2023-11-08" version="1">MNIVKKLQQELETAVQEEATVNASYRKHLIRCARADCLQLTKMMHERLPRELRDLVYQYLLVDERPILAGPYYHFRKYDPHTRQNSLHVGLPRSFDAHDIPRNAIDGFDTDEEEVGLDSDAVILPDGRVKHDHSHKPPSDMVLPNSHVLNPSYVGESFAAEAHEVYYTNNTFSICNVERAIFEFIFTEPLRKFFNRKDDERFTPRAALVRNLQIRIKMENFESTMPFSVQQDYATEEEKYAYERNFLRYHTIYLKYLGNLTGWSRRRELNIEFILMTDLPPNPLPKERIFVNMLQAIRDTVYTNMYIRKGMTIRVTHYDEAIGPFPRDLTGLFSLTKEQWEREQAVHHGKSRWVADFYLAPEPIDVATLGGGFSEREMLPLLRERWGIDSAFDTKCKYPITEGRYWPKGNSRPGWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3D2</accession>
    <name>A0A9W8Y3D2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glutamate decarboxylase 2</fullName>
            <ecNumber evidence="9">4.1.1.15</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GAD2</name>
        <name evidence="9" type="ORF">N0V83_008166</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the group II decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.15" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3D2"/>
    <dbReference type="OrthoDB" id="392571at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004351">
        <property type="term" value="F:glutamate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45677:SF8">
        <property type="entry name" value="CYSTEINE SULFINIC ACID DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45677">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5 7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4365547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="520" mass="55297" checksum="808D7E03C8C70B05" modified="2023-11-08" version="1">MSATNGNSEAPLNRADEVQDLLTAITDLIVPFVAAADQDAATKHTGHGLRVEGSGGPRTTLVEHHPPKKLEGLLQKELDIKGNALGRDGLVSLVESVLKYSVNTWDQGFLDKLYASTNAVGLVSELLLAALNTNVHVYQVSPVLTLIEKRTTTYLASLFNLPPSTSGGISQPGGSASNSTAIVIARNTLYPATKASGNGTHAFTIFTSAHGHYSVEKAANLFGLGSRAVISVPVDAAGAIVPEEFERLVQESKGRGETPLLLNATAGTTVHGSFDPFTPLAAICKKHNMWFHIDGSWGGSVVFSSAHAGSRLAGAELADSITINPHKMLGVPVTCSFLLGADMNTFYKALTLPAGYLFHNAPGAGVEDIYDLADLTPQCGRRGDSLKFFLALRYYGAAHFEQLVANGYDNAEYLLRALKERGNFVTISPEPLPCLQVCFYYAKGGVLGDDAVANGKVTADVASALIRRGFMIDFAPGEKGKFFRVVVNGNTRKGTLDGLVKAIEETARSLGYKLTRLTET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3F3</accession>
    <name>A0A9W8Y3F3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008382</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3F3"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4365762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="194" mass="21934" checksum="D56778DBD40F18E8" modified="2023-11-08" version="1">MKDLSMVVPLGPPSQRVLDEATRILRSGGTLEIWETDHIIRSILPHPPPPPGKNPHEYACAVATGTFLISPITPFTEVQNKYLQDSNTWIQEALDRRKLSPTPCARISQMLLQETETLRDVENHRVAIPLGELRWEREAGKLKKGNSAPDTSTLTEEQAALRYTALLVVIQLIESLEPLLKEVSGKNQEEWQRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3R1</accession>
    <name>A0A9W8Y3R1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_007490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3R1"/>
    <dbReference type="OrthoDB" id="5429770at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053175">
        <property type="entry name" value="DHMBA_Reg_Transcription_Factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38791">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4366960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="487" mass="54361" checksum="966F849273302D70" modified="2023-11-08" version="1">MHIVCSGYRDPLDQNFRDESASVVKRAQKSYKTASHGAVTLKTCTPEVHSDSNYADSRDGTTLELRQRHSMSSPNVLFGLPQSVEKVALAYFMSAYIPGSHYEYLPFMYRQPETEILSATVRATSIASLARETGQTSLTEMARRSYAKALLETNATLANTATTTNDTTLISVLLLSLFEAIAWSSPRPPQSWTTHTQGALALIRIRGSQQFKTLIGRQLFVQVTNIICINSIQQRKRLPPDLVDLIKLAEQTDEGALKYKLAFLTSDVSNLLSDIDEGVMTREEVIRATQSLDAQYVSFCESFPPAWKYQKIRLEKSRPEVYGTIIHRYSSQRIAQLWNSCRMTRILLNEIIHAHALHVPSQYGTPIRTLAAHNLQNMATDICASIPQLIDPSIVPILPDSRMNSPFAPFTESILESTSPKAVAASLLWPLSATRSASLASEDVRAYAVQQLNSLGREFRLPQTENITIGHCEFDALQDGLHMFYVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3V5</accession>
    <name>A0A9W8Y3V5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MFS general substrate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_008000</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3V5"/>
    <dbReference type="OrthoDB" id="10250282at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF76">
        <property type="entry name" value="POLYAMINE TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="403"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="471"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="558"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="589"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="671"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="707"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="756"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="784"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="845"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="881"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="178"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="260"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4366364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="994" mass="111898" checksum="D152357AB5BED6D6" modified="2023-11-08" version="1">MVIEHGVPPAQRTPRVPKAPTPLRNPHRASISHEPQRRLSIVRHDNDELDEDSLYDVRPRADSIAPVPDHLERSITPESIHIPVQRHVGTIAVPSAFESHISISPTQTSPGPSTFKLHDRRISNAVEDLEEMVQEAVEIAEVADDRVQVKEIYEIINEAKSAIQEASGEPMKHLMRTTSPLEVSSSGSEDESDLYSQFKDGERPTNFPPSEHRTSPRLPVVPRDILDDKEYSMHPKSSVGAQRESVSFDWAYPKRKIQPDKISSMSSLSSSDEDERDRNKFSTRSDLLLPPQHAQASPRDHVDFVLRPVNRDQSRGRPRQQFSEDQTTRPRRHYHGKSSSRSRARRFHSSKYDTSLEEADLTAKPYGTELNVREQADRHTFSLRRHHRRQPIARNWSTGKKRVTAAIACINTALLGIIIGVYAGMVPRMQYYLADEKHIVIIGNAVLYFGLAISTFVVWPLPLLHGRKPYLLAALALALPLQFPQAIVISARRSPKDPRFRTGLLCSRAALGLVLGFADVNYITVLLDLFGASLQSKNPHQEFVVPNDVRRHGGGMGLWLGIWAWCWIGSLAVGFQIGAAIIQSLSPDWGFYVVVIILAFALVLNIIAPETRRAPYRKSVTEVYDRDENYITRRVSRGECKLHISTEGPKYWFEEVWAGIKLMIMMMCQPGLLVLSFYLGWIYAQIVLVIVLLGALLSRDYRWQPRYVGAGVMSIAIGAFLALPLTKANIFSRERKRAFRTDSMTFQKQVTWTSHLVRRAIFTLTLPLMGLAYTVSSAGRPRPYMVPIVFAGAVGFLSVLAIAECHGLIMETFDTCDLQPGVNTRHRFESMAVQDRRRRTNYTSFPRVTAGIFVSQTFAFILAGVATLVGGSMTRRLGAQASTGVTAGILMGLTILLTAVVWRFREVQVIPNHTFGTRRDTAAWEEFKQLEKIGRGSDWKAVVIGNPSGKMRRMNVLELGSLSRWTEIRKLNFLIKGPRLGEEKQEGRGFTDGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y523</accession>
    <name>A0A9W8Y523_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_006889</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y523"/>
    <dbReference type="OrthoDB" id="10267969at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF50">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YOR292C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4367308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="224" mass="25234" checksum="EBC7053A775585E1" modified="2023-11-08" version="1">MAAVSLLARKYNSYYAQRPVLTTMITNSVLGGIADTVAQTLTAVRQRAVRKKGGPDKDDFLAIEIHDLDRRHVFSDHDLIPDSKKLPPPFDFERTTRFMSYGFLMSPLQHRWFKFLSATFPVSKAASWIPALKRVAFDQFLFAPAGLAAFFTFMTIAEGGGKRAVQRKFQDVYVPALKANFMVWPAVQLINFKLMPIQYQIPFVSSIGIAWTAYLSLTNSAEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5B1</accession>
    <name>A0A9W8Y5B1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="9">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_008518</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5B1"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000037">
        <property type="entry name" value="alpha-1,2-Mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF89">
        <property type="entry name" value="ALPHA-1,2-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="321"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="435"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="498"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="584"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="392"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4365896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="607" mass="68688" checksum="8293F8151A6FDAFC" modified="2023-11-08" version="1">MALLRRYTLYTAIAVIFVYMLYSLSFPYETEGSPGHHDGDAQQEQRPQRQPAYKPFNWAKVPFKNRIMKLEPLPTGAIKQLPRIQFAFKAEDPTAKKIRETRREEVRKQFLKCWKSYRSYAWMHDEIRPISGEVTNHFGGWAATLIDALDTLYIMGFEEEFSSAIKDIEMIDFGYTDLPKVNIFETNIRHLGGLISSYELSGDKRLLNKAREVGEMLYHAFDTPNHMPITRFNFRAAGEGKPQVADEQVLIAEIGSLTMEFTRLSQLTADPRWFDAVTRVTRMLEEQQSKTALPGMWPIVVNARTADFTRDTGFSLGSMADSTYEYLAKMYALLGGRDAIYMKMYEQAMATAAKHALFRPSTPGESDLLAPGFVRVEGSTVFVDPELQHLGCYTGGMFALGGKLFQKEEHVKIGRKLTDTCVWAYKASPAGIMPEVSHLLKCTNTTGCPWSEKSWIDTVQLKSEAGAKEKDPLRNIANLRLPAGFTAIDDRRYLLRPEAIESVFVLYRVTGEQSYQAAAWDMWTAIQRATDTDIGNSALLDVSAESPPRLDSMESFWMGETLKYFFLVFSEPGVVSLDEWVFNTEAHPMRIPQVRKEAKDGGGKKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y674</accession>
    <name>A0A9W8Y674_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SsDNA binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007609</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y674"/>
    <dbReference type="OrthoDB" id="1078367at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04496">
        <property type="entry name" value="SSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00436">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="16333" checksum="92C6BC241BCFDFAD" modified="2023-11-08" version="1">MFSSLRTSAARAPAAFTTRAFSSTPRSQLARMTVVGRLGVAPEEVTVSGDRTLVRYVVGTSYGKGEDKKTSWFRIASFVQGAQKDYLLSVPKGSLLYVDADARMETYVDAEGNKRSNLSLVARNFDVLSRSQNNEVPETNDEGLVQEASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6P7</accession>
    <name>A0A9W8Y6P7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Autophagy-related protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">N0V83_006167</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6P7"/>
    <dbReference type="OrthoDB" id="310217at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0072">Autophagy</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="40"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4369084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="376" mass="42834" checksum="92E65B54D2EA8654" modified="2023-11-08" version="1">MNQMSTPPWRAKTITVKTVQSDVSIPTHRLSVYSLFTKYFSGKKKLGEGGNGAVHSYEYSVSKIVIAVKTASSGQRSAVEQLRDEIENFKHIGKHDHIVDMLTYCTSYGEPGNNAIFLELCDLGDLKAYADSCCEQEQAIGNPAQPLEITIWKLYKDIALGLNYLHNDLDHCYVHTDLKPGNILVSRPKYWSDADGLPLEPTFKITDFGPMTQYPLPRDAPHYKWAGTPEYAPPLSEQTPPLRPSVDIWGLGATIQFFALGHHPVQSRETCRALRRALGKDVPNSKQKRLWSTTYWRRQRPTMYRPLNVDVEELKGPWDLGRMQDDYEPYSSVLNREYKALWEKDSGSRITAKMLVKDVIPKMDRQIALLKSQEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6U0</accession>
    <name>A0A9W8Y6U0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_007014</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6U0"/>
    <dbReference type="OrthoDB" id="191979at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF285">
        <property type="entry name" value="RETINOL DEHYDROGENASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="288"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="373"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4367432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="409" mass="45753" checksum="32432966A727ABC3" modified="2023-11-08" version="1">MPVNILAQVFSEGLSSVPFGWTFVRAASVCAVLYLLKWFFNGATNGSERNMHSKVVMVTGGTAGIGAEVVRGLAHRGAQIVLLVRQPLSDPFLVDYIEDLRTETNNELITAENVDLESLHSIRQFATKWVDNAPPRRLDMIILCANTMTPPGGKATMTEDGLESTWGLNYVANFHLLSILSPALRAQPPDRDVRIIFGTCAAYIGGKLPDLDSKSSTTRSKKDAKAEPGQINFTPSGVYATSKLALMTFAVAFQKHLSNYVRPDKQPMNARVIIVDPGWTRTPGMRRFLTFGSLWGLALYLITWPLWWLILKSPDQGAQTFLYAAMEAQWGRGEGSYFLKECRRFTWTRKEIEDEVLQKKLWESSEKTIEVLEKESAKRRAVEKKEQEKSQGKSTAKESNGEAKQRKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6X3</accession>
    <name>A0A9W8Y6X3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_005853</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6X3"/>
    <dbReference type="OrthoDB" id="260807at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101:SF57">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="20658" checksum="8DD50D70323BB754" modified="2023-11-08" version="1">MVVVALLYGLHYDIHKAQVGVLGTPEGRRMDRVYSMAPKYKNEVEYLYSFVQVITACTASFAHGANDVGNAVGVWASMYGAWRTGQTVASKEEVPLWQIAVVALTICIGFITYGYNIMKVMGNKITYHSPSRGSSMEMGAAITILIFSQYSLPVSTSRVGLLFFSWVMTIPIAGLIGGGLMGLALNTPSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y757</accession>
    <name>A0A9W8Y757_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">N0V83_006026</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Mycotoxin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y757"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902181">
        <property type="term" value="P:verruculogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11058">
        <property type="entry name" value="CYP60B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000063">
        <property type="entry name" value="Cytochrome P450 monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF210">
        <property type="entry name" value="CYTOCHROME P450 MONOOXYGENASE ASQL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="453"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4368944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="517" mass="58089" checksum="347269D094D2579F" modified="2023-11-08" version="1">MGVFDGDRIWQIALWTPVAFVAYVVLNAIYNLTLHPLAKYPGPLLWRISPVPSIISLLKGRISFDYKVFHDRYGPVVRVMPNELSFNTAKAWDDIYGHRVGLANMDKDPIHVGAVEAIPGATNLTMAPDLHHARQRRALAHAFSKQALLEQEPILKGYVNLFVQKLREMAHRGEAANMVSWFNFCTFDIIGDLSFGEPFGCLSEGEGGESASWVVLIYEAIKSGAIEQATRRFSPPGSLAQRVLMWCIPTAVRERRFRHLSNSTEKTVRRMNTKTEHRDFIWYILKQREKKNEVSDDEVIMNAALFIVAGSETTATELCGLTNYLLRNPEIFKKLKDELRAACKSEADLNMDVLGSLPYMNACIEEGLRIFPPVPIGLLRTVPKGGSLIDGHMVPENTSVCVSSWGAAHSASNFVDPDSFIPERFLDTPESKTKFGRDVKKAAQPFSTGPRGCIGRNLTYVELRLILGALLWNFDLDFADGARLWDPKDEFKGLRAYNTWEKSPLMVKLTDIRKTPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y766</accession>
    <name>A0A9W8Y766_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF1275 domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005381</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y766"/>
    <dbReference type="OrthoDB" id="5288586at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010699">
        <property type="entry name" value="DUF1275"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37488:SF1">
        <property type="entry name" value="DUF1275 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37488">
        <property type="entry name" value="DUF1275 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06912">
        <property type="entry name" value="DUF1275"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="221"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="29672" checksum="AFF5D5A6B0F11848" modified="2023-11-08" version="1">MSNHTSRRDATQRAPDEESPLLGRLPGKDSDSSVTVQRLKKHFAADVTKDWADLVLLFCYIITGLLDSSAVFIWGSFVSMQTGNTVYLGLGLIAPTEGIRWVKAGTSIASFCLGSFCFARFHRYFSPAKRWVLIASYGFQLLLVVLAASIVTFGDDKSNELHWQVLVPLASVAFQSSGQAVTSRALKYNGLTSVVLTSNYCDLFSDPKLFALSNIERNRRIAAPVLLLLGACFGGLWAHSSVGLAGALWTAAALKLVAVVAWVFWKSEKEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y821</accession>
    <name>A0A9W8Y821_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_005791</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4370027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y821"/>
    <dbReference type="OrthoDB" id="424974at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF50">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="5">
        <location>
            <begin position="117"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="357"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4370027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="475" mass="53795" checksum="85944118C575E81A" modified="2023-11-08" version="1">MHVLWEYYVLNVDILVKVLYKPSVQCLIKRASSDLDSLDTSSEALLFAIWFATATTMSRSECLQIFKKERSVLVSRYRNALEQALVQAGWMMSQEIAVLQALVLFLACAPSKNVRLTWMISGTSLSLAQAMGLHSDSASFCLGPIETEVRRRVWWSLCQLDNRISDDCGLETHVPVLSMDTKLPLHVNDADLLLQPCNDARSQEPRMESRTGDPGGFTEMTTTLVKMEFARISLRVKRYDKQCTRLIIAKLGHIIVTHDLLPVPLTHANPNSQQQEKQQAVTSGKMNESLLRYNATVLDITAQFPNDDRNFGWFFRCKYTKWHVMAYLLVELFKHADAAGEAVEQAWEVLHSVFEGGKRGDARGSEEEESKRNEKGEGKENENEKKGLWMPLKKLYMQARQARQHARGGQPGAAPAEALGEETLQVDEDEDGDDDVNDHALLGDPFLGSDLDFDQEISWEQLDAWIMDFEPEAFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y843</accession>
    <name>A0A9W8Y843_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein mS29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_005217</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y843"/>
    <dbReference type="OrthoDB" id="274828at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019368">
        <property type="entry name" value="Ribosomal_mS29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12810">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12810:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10236">
        <property type="entry name" value="DAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="45"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4370696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="481" mass="52542" checksum="78C4AFC70EF87EC3" modified="2023-11-08" version="1">MPPSLCARSFSQLSLDASAHTFVVSRLVHHRVACFSTSTARYAAAPKKKGMSAAPKKGVKSLNTKKGKSASGGDSGKRPAQGERKALRKRIVLSNNNALEVSSLKDLDKANALSEQNGGKVMGLPEPVVDALRAAEAFKTTQGWSLFRRPAVLMRKEAVQIATLFKEVEASASGQQKKTIRRILSGERMSGKSTLLLQALSMGFLRDWFVISLPEAQDIVNAHTDYAPLPKSEPMQYTQDTYTANLLQQILKSNGKFLETTKLTTKPDLPLPLSEKATLKELVALGSANPEASWPVFVALWAELSKPGRPPILFAVDGLSHIMRNSEYLSADVKPVHAHDLTLVRQFIDHLSGQKQLPNGGIVLGATSQSNAPASAALDFCIEVAEARQKTPDNVPQWNPYKNVDVRVMEALKDLNNEKSDFDVIKVGGLSKDEARSIMEYYAESGMLRHQVNVGFVNEKWSLSGMGNIGELEKASVRLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8D0</accession>
    <name>A0A9W8Y8D0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">D-xylose 1-dehydrogenase (NADP(+), D-xylono-1,5-lactone-forming)</fullName>
            <ecNumber evidence="3">1.1.1.179</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">D-xylose-NADP dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_005711</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>D-xylose + NADP(+) = D-xylono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22000"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15867"/>
            <dbReference type="ChEBI" id="CHEBI:53455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.179"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfo/Idh/MocA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.179" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8D0"/>
    <dbReference type="OrthoDB" id="2129491at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047837">
        <property type="term" value="F:D-xylose 1-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050984">
        <property type="entry name" value="Gfo/Idh/MocA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22604:SF115">
        <property type="entry name" value="DIHYDRODIOL DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G07520)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22604">
        <property type="entry name" value="OXIDOREDUCTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="6">
        <location>
            <begin position="36"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="7">
        <location>
            <begin position="141"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00042988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4369947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="380" mass="41747" checksum="C98A1240E9CD6FA9" modified="2023-11-08" version="1">MTSNPFTLRWGILAFTKDLLIDPTTRDAGDIRHVVAAAASSTSASRAQSFLNEAGAPSSAKAYGSYRELVEAEDVDIIYVATPHSHHYQHTRLVLDAGKHVLVEKPITVNAEQCKILRELAKEKRLFLMEAVWTRFFPLSREVTEFVRSGQLGDVKRVFADFSFWNDVEKEFGNQHRMVNLELAGGALLDLGIYSLTWLFMTLYHAQPKDKRGDPQVSSAVTKYEPTGADETTTVLLKFPGTGAQGIATTSIQVATTPNPSLPAPDAIRIQGTHGDLTVNYAPRPRSYTLTPASSSAHGTLASFTHQTKTFDTPGGGHGMFWEADECARCVRDGKLESEVMPVEETEVLMRVMDEVRTQGGVVYPGEIGSVEWPLEGFGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8R4</accession>
    <name>A0A9W8Y8R4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 11">Glycerol-3-phosphate dehydrogenase [NAD(+)]</fullName>
            <ecNumber evidence="2 11">1.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">GPD1</name>
        <name evidence="15" type="ORF">N0V83_005238</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 11">
            <text>sn-glycerol 3-phosphate + NAD(+) = dihydroxyacetone phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.8" evidence="2 11"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8R4"/>
    <dbReference type="OrthoDB" id="10263760at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141152">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000004">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006168">
        <property type="entry name" value="G3P_DH_NAD-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006109">
        <property type="entry name" value="G3P_DH_NAD-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011128">
        <property type="entry name" value="G3P_DH_NAD-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728:SF8">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE [NAD(+)]-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07479">
        <property type="entry name" value="NAD_Gly3P_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01210">
        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000114">
        <property type="entry name" value="Glycerol-3-P_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00077">
        <property type="entry name" value="GPDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00957">
        <property type="entry name" value="NAD_G3PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9 10" id="KW-0520">NAD</keyword>
    <keyword evidence="3 10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent N-terminal" evidence="13">
        <location>
            <begin position="11"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent C-terminal" evidence="14">
        <location>
            <begin position="257"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="186"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="196"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="16"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="334"/>
            <end position="335"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4370717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="411" mass="44846" checksum="C1374FD9385682C5" modified="2023-11-08" version="1">MATLSAQRKHKVTVVGSGNWGTTMAKLVAENVKEKPTLFEETVQMWVYEEDVTFSKDSKHYSGSDKPEKLSQLINQVHENIKYLPNITLPSNVVANPDLPSSCKDSTILIFVLPHQFIARSCSQLAGKILPYARAICCTKGVDVGEKGIRLMSETIGMTLNIYCGALSGANIASEIAQEKYSETTVAYDPPPMDSRNPTPSGSPNASQVDLINPSKPNPGPRSAHLTPLPAEYPPLSHENVRKLFHRPYFHVHMVSDVAGVSLGGALKNIIALAAGFVDGLGWGDNAKAAVMRVGILEMVKFGKAFFGESARTSTFTEESCGVADVVTSCMGGRNFRCAKMAIQRGKTIYEIEQTELNGQKLQGTSTAYEVNEFLKSQGMEDDFPLFTAVYNILEGNNKPEDIPNLIEKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8U8</accession>
    <name>A0A9W8Y8U8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005256</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="255" mass="28864" checksum="02FB8347A85FB130" modified="2023-11-08" version="1">MGQGQSGNTEEPPEEPPEGPPEPPRGDVGFNEFVKKVEQNFAENGGDPRDIIKFRELAHRINMAVDMQEGPTKPRAGPMEPYTMDRMEADNFRMIVEKQKERDARIEQLQKKNPWHLLQMDDNMFCFVMPYGPGGKPGPKPANEAEENKEGLAADVFNLASSKMAEALDDCNNTWVYKRRKPCYPECMQPELGFDHCHCEEIRYWKGIHERDGAEYVSLVVRGGSERDSDRDGLCCSSDHDGVEPMAGEEEPNSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y901</accession>
    <name>A0A9W8Y901_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Small subunit (SSU) processome component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">UTP5</name>
        <name evidence="6" type="ORF">N0V83_005912</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UTP5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y901"/>
    <dbReference type="OrthoDB" id="30195at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052414">
        <property type="entry name" value="U3_snoRNA-assoc_WDR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44267">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44267:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="5">
        <location>
            <begin position="208"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="342"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="342"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="383"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4368830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="452" mass="48608" checksum="DDE597A85A302443" modified="2023-11-08" version="1">MPAAVASRARELKSSRNRDSAPPAKKTKLTQSPAASRQNGLKALVNGTAQKPTAKINGIKNARTDRVDEATAVVGGGQAGHGDVDMADAGNQVIEISSAEEESDEASSSEDEEETPQVGAAPAVNGHVEEDGMDQDGTAEPDELSFGDRLRAQDPEPVQQSRIVDVESTFAGANDESRALATAAHSRPLAAPNASSLGTVLAQALRTNDTELLESCLRVVDVDTIYATVERLPSPLVANLLQKLAERLHKKPGRAGMLMVWVQWSLAAHGGYLASQPQLVKRLTTLNKVLKERASGLQPLLSLKGRLDMLQAQLELRRRNQRKAANDDEDEAVIYVEGEDDYVSSVEGDEEIDDAAPGSQKRTRQDLEDEDESSVDEMPTTMEVDEDSEEGTDDSEDLIDDEAEETDDDIGDDMSEPASDDLDDGEGVSESEEEAKPARRSTAARAGLKSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9G9</accession>
    <name>A0A9W8Y9G9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003769</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4371996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9G9"/>
    <dbReference type="OrthoDB" id="276546at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02933">
        <property type="entry name" value="OYE_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000337">
        <property type="entry name" value="NADPH dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF129">
        <property type="entry name" value="FLAVIN OXIDOREDUCTASE HXNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="1">
        <location>
            <begin position="18"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="414" mass="45391" checksum="34B067F433E76DCC" modified="2023-11-08" version="1">MPKADSPSGFKPLKETALFTPFKLGPLSLEHRIVQAPLTRMRASKESDGVFVPKDLHVEYYSQRATKGGLQLTEATDIAKYASGYPGVPGVFSESQIAGWKKVTDAVHAKGGYIFCQLWHTGRASPPSFRAGAPTVSSSDIPMEGSWIDGVACADHPPKPLSVEEIQDLTKTWGEASKKAIEAGFDGIEIHGANGYLLDQFLHDNVNKRTDQYGGSIDNRSRFPLEVIQACSKAIGADRVGIRLSPYNYFQNTKDSNPNEHWEYLCEKIAALPEDERPAYVHMVEPRFDEVLDEQAKMDALAAYTSKGGKGVEAEATVKGKGNTLVIFRSILQKGGVKFIAAGGFNRDNATPKVESGDADLIIFGRWFIANPDLPKRLAEGLELNQYDRNTFYGADPPQKGYTDYPFYGQTASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9M8</accession>
    <name>A0A9W8Y9M8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Guanine nucleotide exchange factor for Cdc42p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CDC24</name>
        <name evidence="4" type="ORF">N0V83_004380</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9M8"/>
    <dbReference type="OrthoDB" id="1594986at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000935">
        <property type="term" value="C:division septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031106">
        <property type="term" value="P:septin ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05992">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13246">
        <property type="entry name" value="PH_Scd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000364">
        <property type="entry name" value="Rho guanyl nucleotide exchange factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000176">
        <property type="entry name" value="Rho guanyl nucleotide exchange factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010481">
        <property type="entry name" value="Cdc24/Scd1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033511">
        <property type="entry name" value="Cdc24/Scd1_PH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053026">
        <property type="entry name" value="CDC42_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47339">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47339:SF1">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06395">
        <property type="entry name" value="CDC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15411">
        <property type="entry name" value="PH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="2">
        <location>
            <begin position="258"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="3">
        <location>
            <begin position="938"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="591"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="720"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="840"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="877"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="41"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="598"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="624"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="650"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="720"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="753"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="817"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="900"/>
            <end position="934"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4371164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1035" mass="115711" checksum="7937A49C9AC815A6" modified="2023-11-08" version="1">MEGGIAFHSGLARAHTSIPSPSPSSSHSRIASNGGPPALMSHQSFQSQYSSYSHSSVRDTQSTQATSTSTLFGQPPPFPSSTPVNGGPVEASDNVLNKRADKDTSLFQRCLTLQMRLRAIQGFDRWMAEEENKADDDADPVTLLWRTFRRGYPLMELYNALGPRILLSIPNAKTDEKSLKKNEKMATYRFIQSCVNELGIPQESCFILGDLYGDDTTGFVKVINVVNRVLDSLVALGKISETAEIMATAAVTSTKRTQREHIIDELVKTERTYVQHLELLQEFKKLVEEKGVISGDQVHDIFLNLNALLDFQRRFLIRVEQTNAQHATEQNWGNLFVLYKDAFKVYEPYIANQKRCEQIAMREFDKLKDTGGSPEMRQMVESPTLLTSFLLKPFQRLTKYPLLLQQLRDKGDLDEGRREDISRGIEAASSVLAGTNDAIAREERVEAVEELKTLVEDWKGHRIEGFGELLLYGQFTVLKGESMTSKNEEREYKIYLFEMILLCCKEINVNKPKNKMSTRSLVTKDGKPKLQLKGRIFMQNVTETISLQKPGSYTCQIFWKGDPGIENFIIRFTSEETMKKWATQVDTQRRVWKDHARSSASTTASKPSDTQFAYMRDKVLENPYQEEDDEDGEDDVDVVVPGYPRDSYGHRQNSPSASLRSRSTTGESGPPMGDGDPRVPPPRFPMGYNGQPPLTLRTQQIQNAANQESYFSPVAETPMSTYSNARTSSSSNGTFPFPRQAPPNNYSHEENNRYTAPAQTRSHLSRGESGQYPQGGRAMQRPSLPPNANLSTQGRLRAASSPDINSNLPGRRAPNTQQPPVPDVPPFPTHYAYNAAIVNRSNSNSPNTIPPPRAATQSPAIQRDRLIQQRTALELANANNEYHGGTARRDPNYPPPPMQRTMTPASSFERSQGTLTPASMESRNMSPPLSQESSIPTQLKVKVHCPSAGSSMVLVVSTNISFQSLKDRIDAKLQRSTSVSLGSGQVKLKYLDSEGTYVSIQCDDDVQEAFENWKEQQRDLNPGGGLGEIELFCQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9P0</accession>
    <name>A0A9W8Y9P0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcriptional activator HAP2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">HAP2</name>
        <name evidence="8" type="ORF">N0V83_003859</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NFYA/HAP2 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9P0"/>
    <dbReference type="OrthoDB" id="1097733at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001289">
        <property type="entry name" value="NFYA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12632">
        <property type="entry name" value="TRANSCRIPTION FACTOR NF-Y ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02045">
        <property type="entry name" value="CBFB_NFYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00616">
        <property type="entry name" value="CCAATSUBUNTB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00521">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51152">
        <property type="entry name" value="NFYA_HAP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="115"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="246"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="144"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="197"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="319"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="343"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4372086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="358" mass="38557" checksum="B1E427C6082FA18B" modified="2023-11-08" version="1">MTDYAQYQQPHHGHAPGHLQNYGGAPNPAAGNPSITSPTQQQMQYQQTSPILPSQGYGHGGAPNPQHHQQMNYAQQGYIPGMHQQFMSPTQAAAMATAAAAGPAAGYYDQNAIPGQLAQDPRASPRMSGGQMKGDGRVAPRSPTAVSNAMSSALPSQAQMTPGQMQQRRMSAQISSPAMQQPQPVMSHVGPRPSVPPQMTQQPPQHQQSPELVSGAQAEEAPLYVNAKQFHRILKRRLARQKLEDALRLTSKGRKPYLHESRHNHAMRRPRGPGGRFLTAEEVAAMDAQKGGEGGEGNKENDSLPMRAPPSSGPKRKASSTQIKGTPNNKKNKAGAAQRQSTSEEEEEEEDDDAEDDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9S5</accession>
    <name>A0A9W8Y9S5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">VPH1</name>
        <name evidence="12" type="ORF">N0V83_004456</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9S5"/>
    <dbReference type="OrthoDB" id="10264220at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="423"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="469"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="544"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="574"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="639"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="797"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="685"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4371239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="855" mass="97640" checksum="FF1EC3EABA64AAA2" modified="2023-11-08" version="1">MAPPKDTMFRSADMSLTQLYISNEIGREVVSALGELGVMDFRDLNSETTAFQRTFTQEIRRLDNVERQLRYFHTQMEKSSITMRSIYDFNNPFTAPSASEIDELADKSQSLEQRISSLNESYETLKKREVELTEWRWVLREAGGFFDRARGQTEEIRHSLDDDDAPLLQDVEQNGQGDTGAERSFTVMNIGFVAGVIPRERIAAFERILWRTLRGNLYMNQSEIPEPIINPETNEETSKNVFIIFAHGKEIIAKIRKISESLGADLYSVDENSELRRDQIREVNTRLSDLASVLRNTKSTLDAELTAISRNLAAWMVIIKKEKATYETLNKFSYDHQRKTLIAEAWAPTNSLGLIKSTLSDVNERAGLSVPTIVNQIKTTKTPPTYFKSNRFTVGFQTIIDAYGTIKYREVNPALPSIVTFPFMFAVMFGDTGHGVILLLAACAMIYFEKRLERSKLDELFSMMFYGRYIVFMMGIFSIYTGLLYCDAFSLGLPFFKSMWVWDNDGKGPTSSRVEGYTYPFGLDYRWHDTENDLLFSNSYKMKLSILLGWCHMTFSLMWSLVNARYFKTKIDIWGNFVPGMLFFQSIFGYLAFTIVYKWCIDWPARGENPPSLLNMLIYMFLQPGTLEAGVNPLYPGQAAVQVVLLLIALACVPVLLFLKPFYLRYEHNKARALGYRGIGESTRVSALDDDDDEESRPMNGGRESFGDDDDGIAMITQDIGHGEEHEEFEFSEVMIHQVIHTIEFCLNCVSHTASYLRLWALSLAHQRLSIVLWEMTMANAFAVTGVLGAIMMVVVFYFWFALTVAVLCVMEGTSAMLHSLRLHWVEAMSKHFIGDGVPFEPFSFKVLLEEDPVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA10</accession>
    <name>A0A9W8YA10_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Efflux pump dotC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Dothistromin biosynthesis protein C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">N0V83_004025</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Efflux pump; part of the gene cluster that mediates the biosynthesis of dothistromin (DOTH), a polyketide toxin very similar in structure to the aflatoxin precursor, versicolorin B. One function of dotC may be to transport early-stage dothistromin biosynthetic intermediates from the cytoplasm into vacuoles, thereby affecting the rate of dothistromin production.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the major facilitator superfamily. TCR/Tet family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA10"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17502">
        <property type="entry name" value="MFS_Azr1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000014">
        <property type="entry name" value="MFS drug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000196">
        <property type="entry name" value="MFS toxin efflux pump (AflT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF102">
        <property type="entry name" value="DRUG TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G08530)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01036">
        <property type="entry name" value="TCRTETB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="345"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="439"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="481"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="553"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="14">
        <location>
            <begin position="85"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="579"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="579"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4372251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="634" mass="68030" checksum="E4FAE5F4936C5EA5" modified="2023-11-08" version="1">MEPTHDTASQRDIRLPNDLAPHKEMDVATPALSNRSSEVDAAVQEPSGAPKEEQISTATATDGPPAPMGADEEKPQRSKLKITLIMFSLAVAVLLVALDITIVTTALPTIAEQFHSASGYTWVGSAYLIAQSAATPIWGKVSDIFGRKPILLLTNAIFFVGSLLAGVSVNMNMLIAARAVQGIGGGGLITLVNIAISDLFAMRDRGAYFGIIGGVWALASSLGPVVGGLFTQKVSWRWCFYINLPFDGVAFLIILFFLDIKTPKTPLRKGLKAVDWLGSLTMVGGTIMLLLGLEFGGITHPWDSATVLCLIIFGAATIFLFFLVEWRLAPYPLMPLDLFSKRSNLAALGVCFFHAFTFISGSYFLPLYFQAVLGATPILSGVYLLPTALSLSFLSAFTGIFIKKTGQYLPMIWFGMFMMTLGFGLFINFDANSPWSKIIVFQIIAGIGVGPNFQAPLIALQSLVPKRDIATATATFGFTRNLGSAISVVVGSVVFNNVMKTKQPQLAATLGPQAASAFGGGSAGANVGLIQSLPDNHKAVARKAFADSLSTMWILYVCFAAVGLMISLLITKNKLDKQHEETKTGIEAEKAKRAERDAERAERRRKRASKGSLPVDTEAQSSTAVVEAEKEIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAE2</accession>
    <name>A0A9W8YAE2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquitin-conjugating enzyme E2 Z</fullName>
            <ecNumber evidence="3">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">E2 ubiquitin-conjugating enzyme Z</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Ubiquitin carrier protein Z</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Ubiquitin-protein ligase Z</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">N0V83_003940</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAE2"/>
    <dbReference type="OrthoDB" id="1926878at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23809">
        <property type="entry name" value="UBCc_UBE2Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00195">
        <property type="entry name" value="UBCc_UEV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000126">
        <property type="entry name" value="Ubiquitin conjugating enzyme, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116">
        <property type="entry name" value="(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116:SF26">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2 Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="15">
        <location>
            <begin position="4"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="UBC core" evidence="15">
        <location>
            <begin position="286"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4372167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="438" mass="50536" checksum="07E6E65CD7860C1D" modified="2023-11-08" version="1">MSNQSILRITRELSEIQRGSDLSLAVACRDIDVRHVRALIIGPPDTPYEFGFFEFAVKFTRDYPTKAPNVTAITTNGGRTRFNPNIYAGGKVCLSILGTWRGERGEEWSSAQGLESILISIQSLMSTNPYENEPGFEDANSEYDKKNQEAYVAKIRHESLRISVIERLEEYMGIHQDRPGVTVYNAEEDYQSSRDDAPFEPFKDLCKRRFLWYYQSYLLRVDEASKKHKDGDRFEKMPFEGPGNTMEGTFQYTTLRERIVKIFESLNKEMEGWAVEGMSAVHREMSIASNLQRQYEQIVEKYKKNDAVTLDLDLVDKNPFVWQLVLFGRPMTNLDGGMFRIMLYISPKFPDVLPRVKFDTPIFHHRVSPDGILCYDAARKDDMRSHIEAIIAAIEEESPAYDPRTLVNPEAAKLFWGAPDEKKLYNRRLRRSVQDSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB23</accession>
    <name>A0A9W8YB23_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CTR2</name>
        <name evidence="5" type="ORF">N0V83_004274</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB23"/>
    <dbReference type="OrthoDB" id="161814at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0187">Copper transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4372500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="91" mass="10680" checksum="6CE9AE9B60049E2F" modified="2023-11-08" version="1">MNTRCQMLFTWDTTDLCIVFRGWHIDGTTSLVFSLFAIVLLTAGYELVREVSRRYEEYKSRGLESGRRDGDDVRGEFESLILYCWVDGWRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB38</accession>
    <name>A0A9W8YB38_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004668</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB38"/>
    <dbReference type="OrthoDB" id="3793201at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="661"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="763" mass="83763" checksum="3714794B10D233D8" modified="2023-11-08" version="1">MTPRHSGINLLPTPLKTPYHAPFLRAIGLGVVNSLLARLQSRSKAEEPKKSIAKKSFWVALQRCAVHLLPCAVSLIVIVLNLKGYFIGFELAGLPNRTSQYMALLQVAAKAQELLIVASLATVVVHQLRHDLIKGPGVPFGLVGVTSLFSQLSFFWSPTFIGSLTRKSGPSKRLFILILLVGAIAATAGPAVAILVIPRESIWPAGGSKYWVSGTAEDLWPSQVGLEHYMPEAQYGIAKAKCNAANAYTNSLCPSGGYMGLLQRITLAYDKPASSGARDAWYSFVNLLRAGYGTLVTSPPSSGQMTPYMMISAKRGILALESSAVAVHGPSAWIAYELEGNWYDAAMSEPNNPGVQSSRYKYYDTAMTTVSTFVPAVRVVCSPPKPLISGDSKVDFPWTPDFEPAGELENKNDIGSGRLRRLRTLNLEGSSLHNRTFSNFPKVSRVALSDSDLTNSTMGFVFEYPWSNNSGRNVSGCVLDMRWAKGVVLQQRDSVSRPALLDPHTIDRDPYMFNDLFSLNPCSVKSRIEISEELFDAVNFPLAPDLKEVAYDMPTLIRPPNTTAIETLLMTIDWNDDWDDGGYIPVFEQAVAILFADALARANAWRMSPQGPYPQKKPDWKAQILNDGEAFDSPPSNPTTTFSMKQQISGYTYNMDTWADWLSIVVLLIHLVVATGHTILLLVTHRSSSCWDSLPELISLAQQSTPSTVALRNTAAGIYRLSTFRHIARIRVSEEDAEHVELVFDVDHGKDSGRQPDAGQKYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBG3</accession>
    <name>A0A9W8YBG3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004866</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBG3"/>
    <dbReference type="OrthoDB" id="433474at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF8">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="45"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="274" mass="29905" checksum="1C73B5D391FDFF1D" modified="2023-11-08" version="1">MIRPELPADVEQTIYHVKGSDGYEIPVYHLRKKNVPLGSGQTPAILHMHGGGYIGVSAEDAVPSIAAYVSHSGIPIFSVDYRLAPENPFPVPLEDCWNSLLWLHSQAEKLDVDVRRIAIMGESAGGGLAACLAPLARDRELSPPLAKQILIYPMLDDRLKSDHTGGLAIFGVEDVITGWAAYLGPNYDTDNVSPYSAAARLTNVKELPPLYLDVGHLDMFVHENLAHVQKYVAASIATEFHLYTGVPHAFHRFAPTAFVTKRAFANRVGAMLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBX4</accession>
    <name>A0A9W8YBX4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Microtubule associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003563</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBX4"/>
    <dbReference type="OrthoDB" id="193478at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018750">
        <property type="entry name" value="DUF2306_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10067">
        <property type="entry name" value="DUF2306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="40000" checksum="A8C4D2E664EB3BF2" modified="2023-11-08" version="1">MVATTRPPRNAFDTFMKKIYSPLGFSKAYNFVLWSIFAGALLGFVLARVMYLNFSTHFCPKSGPSHSGNSAAPGECYYYLNFSRYKIGILLHLAGVFPAALLAVLQFTPFIRHRWIIIHRISGYFAILLYTVSLVGALMIARNTFGGGLDMQAWVGFVGIGVLVCFVISYINIKRLQIEQHRAWMLRGWFYAGSIITSRFIMIIATMIIANQGYYAVWPCAKIEATLPTPHLLLVRYPGCAAYANGTSPNQVAAVAATFGEGDATNSGAALNVNFGMALWLAFAIHALGVEVYLHLTPKEAQRLRQVSYQRQLEAGMRKPGSAGLTVDRLGDAEDWVPEAPRNESVDTVGSGSSKTKRCEETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBY3</accession>
    <name>A0A9W8YBY3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">D-arabinitol 2-dehydrogenase [ribulose-forming]</fullName>
            <ecNumber evidence="5">1.1.1.250</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_004655</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; D-arabinitol metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.250" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBY3"/>
    <dbReference type="OrthoDB" id="47007at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047038">
        <property type="term" value="F:D-arabinitol 2-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000240">
        <property type="entry name" value="SDR family oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF4">
        <property type="entry name" value="CHAIN DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G08710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00066831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00070881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4371438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="300" mass="32188" checksum="F28352C549DAF4A1" modified="2023-11-08" version="1">MPDNADNRASLVNRQLPEMVLSTEENPLTAPRPPPEDLPVAQRAQYRFQVKGNAIITGGAGTLALEAAQALLEHGASGLALWDMQPDNAFDAIKALHAKFPDRRIMTEAVDVRDEAAIASAVESTVKVLGSIDILCCFAGVVGCTHAIEMAADEWRRTHDINLTGSFLCAQAIARQLRSQGTGGSIVFTASISAHHTNYPQPQAAYNSSKTALLSLAKSLAAEWSSYGIRVNCLSPGYMNTILNEGDGLKRARESWNGRNPMGRMGHPWELTGPLITLCSNAGRYINGTDIIVDGGAMVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBZ0</accession>
    <name>A0A9W8YBZ0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2-(3-amino-3-carboxypropyl)histidine synthase subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">DPH2</name>
        <name evidence="9" type="ORF">N0V83_004670</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase. Facilitates the reduction of the catalytic iron-sulfur cluster found in the DPH1 subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the DPH1/DPH2 family. DPH2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBZ0"/>
    <dbReference type="OrthoDB" id="449241at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11860:FF:000001">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010014">
        <property type="entry name" value="DHP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00272">
        <property type="entry name" value="DPH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF2">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01121">
        <property type="entry name" value="Diphthamide_biosynthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00408">
        <property type="entry name" value="Diphthamide_biosynthesis_famil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="93"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="432"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="551"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="96"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="441"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="465"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="497"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="556"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4371453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="592" mass="65010" checksum="2D5C805BF6AA7A2E" modified="2023-11-08" version="1">MRAYANMAVPALATAPALSTPDTHIFEDPTPAVDVSSLPRLSDEQITLTYEIERTVKEIREGRWRRIALQFPDHMLTDAPRVYEHLTRGLGKARKSQNGALRNGEQNGTPTETELSAQLGSSSIQENREDEERLFILGDTSYGACCVDEVAAEHVDADVVVHYGRSCLSPPSRLPVIYVFTERPLDLDPVLSTIRETYPDKETKIILMADIPYSHHIPTLHSLLRDEGYADIFATEIVHDPSSLLPNRTVPSETMQEKEALCDYALFHISDPPTALLLTLSSRVASVHIYPTTSTTTTPSAALASTSLALRRRYALLTSLSTTPIFGILINTLSVKNYMHILSHVQNQITSAGKKYYTFVVGKVNAAKVANFSEVGGWVVIGCWESSLIESREFWRPMITPWELGIALQGDEERVWTGAWEADFEAVLSAEKNNNKDEKTNGASNGVSNSQAKDDEPTNGIGDDAATDDYDSEEESAPPEFDLRTGRYVSHSRPMRSKNTVAKESPQDTQNSRAASSSTALTKRANGDIASVGGVASPGAEYLRSNRTWQGLGSDYRSEEGQDGVDGRAAKMEEGRSGIARGYVVGDEGRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC10</accession>
    <name>A0A9W8YC10_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phenol acid carboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_004013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC10"/>
    <dbReference type="OrthoDB" id="4415004at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008729">
        <property type="entry name" value="PA_de_COase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40087">
        <property type="entry name" value="PHENOLIC ACID DECARBOXYLASE PADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40087:SF1">
        <property type="entry name" value="PHENOLIC ACID DECARBOXYLASE PADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05870">
        <property type="entry name" value="PA_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4372239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="26405" checksum="042F183B14970B74" modified="2023-11-08" version="1">MPNNLRPVDPSDYIRSVFYFFNIGKGKKEIDQKVLAVIQKSNTNIALSTMAPGEHLPDFLTNTPLHPSFDKDILDTHLIYDYDAQDDNGNPEKWRYELWCFSNDRVVYAIHGGPMAGRINYQRATYQCIRPGELWQINWLEETGTIVSAVYDIPNKKMSTMIAFSEGHWKRAKEALGDKRKREDLERWRKLAEVGTQTSRFMLSEQGDVVEVFKGKGDLVPIKESDPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCE1</accession>
    <name>A0A9W8YCE1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_002553</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCE1"/>
    <dbReference type="OrthoDB" id="4940293at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.100">
        <property type="entry name" value="YAP1 redox domain. Chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013910">
        <property type="entry name" value="TF_PAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023167">
        <property type="entry name" value="Yap1_redox_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF8">
        <property type="entry name" value="AP-1-LIKE TRANSCRIPTION FACTOR YAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08601">
        <property type="entry name" value="PAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111430">
        <property type="entry name" value="YAP1 redox domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="5">
        <location>
            <begin position="106"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="55"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="88"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="122"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="137"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4373814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="298" mass="32813" checksum="6C6B9690819FA402" modified="2023-11-08" version="1">MDYAYFGAPQQPYHHFMGMPSNPFPHTAVDPEAIRSVEPLDPTLFPAPYDAFTFHGLPTPQQGSPDAAVAQTPMPVGSLDSGFGGELDDGRASRTRSSSEEKESLTPAQSRRKAQNRAAQRAFRERKERHVRDLENKLSSLESNTHSLQSDNERLKLALQRAQTENEILRATSGQSPSASRPVSVSYPSPGAHLPLEEDAKDSDYNVQSFVNGSVVNAADREHPASRRKPRGKEIPADKAWDFMQSHPLVKQGLVDIADVCERLKGAAKCNGHGPVFDECTIWQAIEESRRCGGDELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCG2</accession>
    <name>A0A9W8YCG2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">37S ribosomal protein S22</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RSM22</name>
        <name evidence="9" type="ORF">N0V83_002578</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial ribosome (mitoribosome) assembly factor. Binds at the interface of the head and body domains of the mitochondrial small ribosomal subunit (mt-SSU), occluding the mRNA channel and preventing compaction of the head domain towards the body. Probable inactive methyltransferase: retains the characteristic folding and ability to bind S-adenosyl-L-methionine, but it probably lost its methyltransferase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCG2"/>
    <dbReference type="OrthoDB" id="421327at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052571">
        <property type="entry name" value="Mt_RNA_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015324">
        <property type="entry name" value="Ribosomal_Rsm22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184:SF5">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09243">
        <property type="entry name" value="Rsm22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="619"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="767"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="157"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="767"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4373839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="799" mass="90194" checksum="BC84BDA75D16C565" modified="2023-11-08" version="1">MLKTRSVQSVCASCRLNFAISRARNARLPAQVQLVPRHWRSRATNFLTPRRTLSTTVVRNQLSQENVAAEDLNVEVNVRRARQLFGDSLPRDHLSDEEYTVYERLYGTPVFVDKIEDLQELDSVAEEDIIEEERPDGSIVLLRRGEDGELEELEVIEETEDTVENEEAEPDEKTMTPDEWEAYRERQAHTYHEGVYEKSRLAQEEAEAWEDMEEEEDFMRAHPLTIAGRFKPSPSTVFLPKEALVDPTSLLLTRANHKHLAESANRIFGGRGLPYSASTPPARLGKEQKPIPLDPSQADMGNIEADAYMATVQPGTYASIMSALVEVRRRLGTSWLEHMLRNKKGGTILDAGSGGVGVLAWHEVLQAEWQRMHEESGNTSQDATPLGKATVLTASDTLRHRASKLLENTTFIPRLPETVTADGEANTQQPRKFYDLIIAPHTLWPLRQEYLRKEQIEKYWSLLNPKGGVLVLIEKGLPRGFEVIAGARSHLLNKHIASPDSETIEASVDSQVSRPDEDTRFTDKETGMIIAPCTNHSTCPMYQSPGVSTGRKDFCFFSQRYIRPPYLQRILNAKDKNHEDVQFSYLAVQRGRDQRLSEHDIIGKGFSQSEASTAAAFEGHEWNPSSDPDSNPELEEPVMSSVQDVNPLTFPRLILPALKRRGHIILDVCTPAGTLERWTVPRSFSKQAFRDARKARWGDLWALGAKTRIPRSIRLGRPSDEYDKKGRKVRKAKKVVIEIGVGEKDGRTVEEGEARVLGKGGRMLVGDRDGRYTSDRKIRGSGGRKGRRKKIDHDVLDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCL4</accession>
    <name>A0A9W8YCL4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphodiesterase</fullName>
            <ecNumber evidence="3">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PDE2</name>
        <name evidence="6" type="ORF">N0V83_002668</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="3">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCL4"/>
    <dbReference type="OrthoDB" id="546632at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="5">
        <location>
            <begin position="252"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="596"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="652"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="671"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="722"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="752"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="797"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="828"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="852"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="893"/>
            <end position="907"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4373929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="919" mass="101325" checksum="53DF72FED242219C" modified="2023-11-08" version="1">MAARTSDGNVGQSYFHLGKTSPPEVHGNVEAILSMFNEVHICGSGKAGLSKIANVLESTKANVPTFVIIDIPYDEERRLKRLSREPRTPSPTSRLFRTEAEPDDTYAMHLLTHIHSEIASRNFSKLVVPVVMLTGLDQEATSALPSPSLQVSHPLTDSVRLTRYIDAGAFDVLSSPLSKDRIHGLVLHAYRLQKEWAREEASFLTTKRNRKLSWVGVDDTKPYAYLREAMVSNLMTGICNPETVGDSLEPTDFDLDQDRKAFVEEAVGTWAFSAHDFTDDELLYGALTMLKHALQMPELAKWAITEDELIVFLLASRTAYNEFVKYHNFRHVVDVLQALFHFLVQIGTLPQYPPSDVKQNSKSPIAELLKPFDALTLLVSAIGHDVGHPGVNNAFLVALNAPLAQLYNDRSVLESFHCAAYSQILRRYWPSAFADAAMRKLMINNILATDMGLHFKYMSDLGSLQQKVAHDKGVLDGWSVKVQEEQKDLTCGLLIKCADICNVARKFETAAKWANILTDEFSNQGLMEQELQMPSCLFGGPPVREDIIKLGESQIGFMNIFARPLFEAVTDILPAMHFAVDEILTNKAIWEKKIDDERQKSKKSKNLAANLSAQNFPIDRTPSPFSDGPAQPAASVPALPTVHSATKLALQDEADRRGSTGSMHGIVPGSRRSSAAEQKTLVSHENQSPSRRGSADPSLTTILVTQTPNVSDAPPKGSSPSPEDRARGDRKDTLTSSSSKKKEKDMGRPVTAPSQARRSQVVSLYPVPQTLPQSHSSEVDLSHTANGSHDGSKLPQWDNSKLSGDSSATRSDASRNSSWWRQMSTRRGTRDIRNGDADGHSQHKDALLNTYPSNTDSTATSPTSPSPSRKTTTGKIKNFFKRKPSSNKEQEKQLSSYGSSSQLRTPPTSDPGRSLNSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCL7</accession>
    <name>A0A9W8YCL7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_003603</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="5">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carotenoid oxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCL7"/>
    <dbReference type="OrthoDB" id="407010at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010436">
        <property type="term" value="F:carotenoid dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016121">
        <property type="term" value="P:carotene catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004294">
        <property type="entry name" value="Carotenoid_Oase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543">
        <property type="entry name" value="BETA-CAROTENE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543:SF24">
        <property type="entry name" value="CAROTENOID ISOMEROOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03055">
        <property type="entry name" value="RPE65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="535"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604294-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4373309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="549" mass="61527" checksum="7846E5237616C29D" modified="2023-11-08" version="1">MVAATADSWPNDSGFDTSYEEHEPVELTVKGNIPHYAAGVLYRTGPLGYKAKTDDGKTWAAKHWFDGFSCVHRFQIDFPEQNSPAKVQYRSRRTVDEYLEIVRKTGKLDSITFASKRDPCESFFKKVMSIFQASNARNAKNVGVTLSINMPGGGYVKNPEKPSINGHTNHIETLHAKTDAFLLKKIDPETLEPLGIATQKILHPDLKGPMSAAHAKSDPNTGDIFNFNLDLGYNSTYRVFRTSAATGKTDILATFKGKPAYIHSLFLTENYVVLCVWNSHITWSGISILYNKNIADAIAPFDLKSKATWYVVDRKDGKGLVATYESDPFFCFHSVNAWEEPNETDPKKTDIITELSMFENTDVIHRFYYDNLVSSIANPEYEGKKRLSCLPMQAQFRLSSVDARVHAGKPLPAELLFQADKMISMELPTINPSYLTRRHRFSYGCADRLKSSFMDGIVKFDNTSQQSIFWEVEGHTPGEPIFVADPEGQAEDDGVLLTVVLDGFVERSYLLVLDARDLKEVGRAEMSGPMSFGFHGAYKSMSRKYAGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCQ4</accession>
    <name>A0A9W8YCQ4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCQ4"/>
    <dbReference type="OrthoDB" id="3732509at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="294" mass="33343" checksum="06639BBEA673E8E2" modified="2023-11-08" version="1">MTKMVQLHCPSTGQTVDKFVISPFQTHEQVIQGIRIRLGIQHAALYTTDAKLITNFDSLQEDQRVLVAATSSELMLPDAPTGFILYDGEESDEVDPTTEGFEQPWEDLTEREKCDHILSLVEQKPTTRNKLRITRPYQSVQPDLFTMHLNSISPTEAEALIDQRWRTTVEHFLPDALKPAKPKTSGKFWDEQVVATLSVLSSFTHGQSRLAREFLEEAVSMRMERSVDDDKDSIVRGQDVIDAVALVYERAGVIPAKLTKHKSAKVKQKERRKAEKEKAVKEKKNAEARRGSGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCT9</accession>
    <name>A0A9W8YCT9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cochaperone prefoldin complex subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">GIM4</name>
        <name evidence="4" type="ORF">N0V83_003970</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCT9"/>
    <dbReference type="OrthoDB" id="29646at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23163">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000002">
        <property type="entry name" value="Prefoldin subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027235">
        <property type="entry name" value="PFD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13303">
        <property type="entry name" value="PREFOLDIN SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="10"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4372196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="117" mass="13684" checksum="128E043CF84FE554" modified="2023-11-08" version="1">MASQAQLKKQQELQTTYQNYKNTLQTIAQKIGDIEQETEEHKLVLETLEPLPGDRKCFRMINGVLTERTVKEVVPILQTNSDGLKKALEELVKQYKTRQDEMEKWKKNNIQVVQQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD67</accession>
    <name>A0A9W8YD67_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD67"/>
    <dbReference type="OrthoDB" id="3798482at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="238"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="334" mass="37892" checksum="8C4DDF2D519CF26C" modified="2023-11-08" version="1">MSLKVDDQGNPPSMAEILRIVQLMREYISGDRRFDQQNAQIDSETCGQADAADIARGMHRYIGSRLRAERIETFCNAVEHNLQTQGHTDDHENSLSVVKNPFKYFGFTIHPATHAIAHGSQAVTDRIMTLVVDVGKWAFTKPDGTPIFSMKGYTICHLTETIECHLGEELFCRIGGAYYCAGRGFNIAHAGSSTSGDLGELQHNIAVEKWKECDQFRGNLDFHNGQLRQEIEVNLPAYQGHLKELDQAEQAAKSRRNNLRRRRDELKAAIAENNTRDWIYRQKFLFSCLPYEFASRRLLVMLGYAQNISKATICTYFVKTKDTNAYTLFIPSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDE2</accession>
    <name>A0A9W8YDE2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PHD type zinc finger protein with BAH domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SNT2_2</name>
        <name evidence="2" type="ORF">N0V83_004270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDE2"/>
    <dbReference type="OrthoDB" id="336088at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048189">
        <property type="term" value="C:Lid2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036205">
        <property type="term" value="P:histone catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029617">
        <property type="entry name" value="Snt2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47672">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SNT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47672:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SNT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="336"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="440" mass="47110" checksum="593812E9AF921D32" modified="2023-11-08" version="1">MRSGYIFGFDMTPVKASRRDAVPTVTLNGETGTLAAAIWCKEHAPKIAVHSMNEPVEGSDQIALQLFAREFKQADLTLTGTARKANLVDQSTRIVSQPAPTQVNRRASAVTAQTPTSARGRPSIAGLPVKEESSEPPIPRPERKCVRCKIEASPRWWKVDETLPTQNAPRVVDGPLAPNGTEPNANGERKPNIENGQIVNGSTDHQMPDAPPVVSAPPQNHLRLDTDVAVVRSASYLCQKCHWKKQNGADDEEERSRSVSVVPEPQHLALRSPPVQPYAPPALAGPWAVPGAPLSMPPNQPPPLPAWHSSVPPPGPPSHHLLNGNGYPAPPPLQGPPVGHAAPYHAPYPQPNGYPPYTGPPIHSQMPPAPLRVSYPPPPVSGPPPPLHLNNGAMLVNGMQSPRTMPYSPTHPHAHHSSRSTESPFTAPARGPAVPSSPTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDE4</accession>
    <name>A0A9W8YDE4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NADH-ubiquinone oxidoreductase 12 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_004032</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDE4"/>
    <dbReference type="OrthoDB" id="10252718at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039993">
        <property type="entry name" value="NDUFB10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13094:SF1">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13094">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE PDSW SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4372258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="12423" checksum="A83786F7FE49354D" modified="2023-11-08" version="1">MPTPESEMFLAKKPKVAPTFTGVDYDDNQALKAAQDSIIREQWVRSMMARLVREEMGKCYRREGVNHLEKCGHLRERYFELLKDSKVKGYLFAQFNYIDEPFKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDF8</accession>
    <name>A0A9W8YDF8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_001791</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDF8"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000117">
        <property type="entry name" value="MFS hexose transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF3">
        <property type="entry name" value="HEXOSE TRANSPORTER PROTEIN (AFU_ORTHOLOGUE AFUA_8G04480)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="324"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="419"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="448"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="34"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4374716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="508" mass="56310" checksum="CCE40F2C693EBE0D" modified="2023-11-08" version="1">MKDADIVGAAIAEVLPQTNKYWFQIPHLLRLNLILLIPLLSSAVAGYDGSLMNGIQSLTQWQEYFGHPTGALLGLVNAAQSGGSVVVLPFVGILSDKYGRRIILFSGLIGVIVATIIQATSTTLAQFVVSRFIVGAAGMFVVQPSPMLIAELAYPTHRGKYTSAFWTMYYLGAILAAWTTFGTQDRQSNWSWRIPTVMQAGYPCIQLAFFWVVPESPRWLVAQERNEEAQHILAKYHSGGDENSPLVTREMSEIVQAIRHEVAAKSVKWSALVATPGNRKRLFIAVCLGSFAQWNGIGVVSYYLTLVLNSIGITDPFYQTLINGLLQIFNFAAAVSAALLVDRLGRRTLFIWSGIGMLISYIVWTACSAVNNEQNNKAAGYVVVVCLFIFFFHYDIAYTPLLMSYPTEIFPYSLRSKGVSCELLSIYVSLVIQAFVNPIGMENIGWKYYIVFCCLLVVFLIVTYLCFPETKGRSLEEIAEIFDGPGASPDVDLVEKDTQYTKEHNEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDX3</accession>
    <name>A0A9W8YDX3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TPC1</name>
        <name evidence="14" type="ORF">N0V83_004287</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDX3"/>
    <dbReference type="OrthoDB" id="18574at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090422">
        <property type="term" value="F:thiamine pyrophosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000011">
        <property type="entry name" value="Mitochondrial thiamine pyrophosphate carrier 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="94"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="189"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4372513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="295" mass="31940" checksum="3FF54676A29EBF76" modified="2023-11-08" version="1">MSFVIAPLDVIKIRLQLQIHSLSDPLSVRDVNGPVYKGTLGTLKQILREEGVTGLWKGNIPAELMYLAYGSIQFSAYTYMSHILEGVPAPYTPHNSAISFISGATAGAAATAATYPLDLLRTRFAAQGKDRVYTSIIASVKHIAQHEGPKGFFRGLGAGVSQIVPYMGLFFASYESLKPVMADSPIPLPLGSSDAVAGVVASVLSKTAVYPLDTTRKRLQVQGPLRDRYVHRNIPTYTGVISTITRIWKHEGRRGLYRGLTVSLLKAAPASAVTMWTYERAMGIMLALDEKSAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEC2</accession>
    <name>A0A9W8YEC2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein mpe1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MPE1</name>
        <name evidence="10" type="ORF">N0V83_002370</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEC2"/>
    <dbReference type="OrthoDB" id="106784at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16620">
        <property type="entry name" value="vRING-HC-C4C4_RBBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014891">
        <property type="entry name" value="DWNN_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033489">
        <property type="entry name" value="RBBP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025829">
        <property type="entry name" value="Zn_knuckle_CX2CX3GHX4C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439:SF0">
        <property type="entry name" value="CELL DIVISION CYCLE AND APOPTOSIS REGULATOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08783">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13696">
        <property type="entry name" value="zf-CCHC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01180">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51282">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DWNN" evidence="9">
        <location>
            <begin position="5"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="8">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="334"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="404"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="648"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="334"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="376"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="404"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4375284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="678" mass="72595" checksum="3498E5C3608B6098" modified="2023-11-08" version="1">MSSSVFYKFKNSREPERIVFDGTDITVFELKRDIITASGLGDGTDFDLHLYPEDDPKAEYDDDTTTISRSSTVIVVRRPAARGHGRAARYVSGKAPVRAIKKETKPTVPIAGATLNEADAEAAFLAESAQVWDAQKETLAHAKPVFHKKKPVNVPTHEPPPGYVCYRCHQKGHWIQACPTNSDPDFKPTIRAKRTTGIPQSFLKKVEKPVDDEDARGVMMNADGEYVQVMTDNRTWEKFQEKANASKAQAASADAASKEVRERGLECPLDKRMFVNPVKTPCCGKTYCHECIDNALADGDLICPNCAKEDVLMDDLVPDEEMVKSIKAYEAEKAKEKLDREKEQAKAEAKAAEQPAAATASPPIKNETESSAVPVETSSTTTASNNDTTTSSLTTTVAATTAAAADPAVEASSATPGATGGNGNASDTESTTSKKRKSPPTEIKPPTAPKAMRQQKEQAANPAAAMEQEFIQQMEALKNGGPAMPLPMLGGVPNMPSMPNMSNMGNMPNMPNNMGFPGQMQMPGFNNMNGPMGGFNPMMNMNMNNMNMMNMNGGQMMGGGPGGGMAMHHQHNNNPQMNGYGNGMGMGMNHQQQQWQNNNQNNGYGGGGGGFGAGMNGYGGQAGMGMGGMGMGMGMGMMNAAGGGAGGADYNDAYERQPVNPRGRHRKAARAPDYRHVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEI8</accession>
    <name>A0A9W8YEI8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_003441</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEI8"/>
    <dbReference type="OrthoDB" id="1431934at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20335">
        <property type="entry name" value="BRcat_RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20336">
        <property type="entry name" value="Rcat_RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="94"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4373150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="389" mass="42410" checksum="E620ECA3D15F0389" modified="2023-11-08" version="1">MARTKQTARKSTNGMSNGRRVILPRNQFTPAAVITPQPPSTTRVLRSDTAKAAAASTSKVTTRSTRRRRALDPWTPKRKVNPNPKPRPRAPPPTHFTCRICIEEQPVDHFVRWFPPKRPGRGRPTGPGEVPFHCVAHLARNPRRKHIDPVCKTCIGLSLSARLDTLGARAIGTGCLEPGCTEPWSHEHLMTYMPRGAALEKFNMDMFAVWLQDADPKPMTCLAPGGGCSAIGVPDASAPGYPQVTCHTCSFRSCAVCLLPWHKDLTCAENGANRINEQISDPEKDTLKLMQTKDGKRCPNCYLVIEKDGGCDSMFCLGCKKYFNWATAASAVTGAKKAEPVTHSNPYWHVNNGPVVCEMDRIEGVASGVFSGIVNGDGVPFVRATADAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFF9</accession>
    <name>A0A9W8YFF9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Phosphoribosylaminoimidazole carboxylase</fullName>
            <ecNumber evidence="4 11">4.1.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_001091</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + H(+) = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + CO2</text>
            <dbReference type="Rhea" id="RHEA:10792"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="EC" id="4.1.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">In the C-terminal section; belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.21" evidence="4 11"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFF9"/>
    <dbReference type="OrthoDB" id="15425at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004638">
        <property type="term" value="F:phosphoribosylaminoimidazole carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000037">
        <property type="entry name" value="Phosphoribosylaminoimidazole carboxylase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01928">
        <property type="entry name" value="PurK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016301">
        <property type="entry name" value="Ade2_fungi/plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005875">
        <property type="entry name" value="PurK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040686">
        <property type="entry name" value="PurK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054350">
        <property type="entry name" value="PurT/PurK_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004679">
        <property type="entry name" value="PRK06019.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01161">
        <property type="entry name" value="purK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609:SF5">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609">
        <property type="entry name" value="PURINE BIOSYNTHESIS PROTEIN 6/7, PUR6/7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17769">
        <property type="entry name" value="PurK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22660">
        <property type="entry name" value="RS_preATP-grasp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001340">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52255">
        <property type="entry name" value="N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="13">
        <location>
            <begin position="108"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4375814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="567" mass="61273" checksum="7A580C29EDE6540A" modified="2023-11-08" version="1">MVDNRKVGVLGGGQLGRMLIEAANRLNIQVNVLDAASSPAKQISAHDGHIEGSFKDSAAVKTLAQASDVVTVEIEHVDTHMLEEVASEVVVEPSWKTLRTIKDKYAQKHYLKEHGVDVADSIDLEGKGVEGLKEVGTTYGYPYMLKSKTEAYDGRGNFVVKSDADVDAAFEALGKRPLYAERWADFRIELAVMVVKTKDGVLTFPTVETVHEDSICKLTYAPPRNVSESTAQSAQALAKKAIGAFEGKGVFGVEMFLLKDDSLLINEIAPRPHNSGHYTIEACPISQYDAHLRAILDLPISQSNLRLREPSIMLNILGGQTPDSHVQVARKALESPNASVHLYGKGDARPGRKMGHITVTAPTLAAAEHEIHPLIDFVDAKKGKKTTTPSPRSETSLSTKPEPLVAVIMGSDSDLPVLKAGLQILETLHIPFTTRITSAHRTPDWLRTFSKSASATSLRVIIGAAGGAAHLPGMCASWTTLPVIGLPVKATHMDGWDSLVSMTQMPRGEPVATVGINNSTNAALLAVRILGAEDREIRERLRVWMEGNEKEVLRKDGVLLGGGGRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG08</accession>
    <name>A0A9W8YG08_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">5-oxoprolinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_001343</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxoprolinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3643at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049517">
        <property type="entry name" value="ACX-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF2">
        <property type="entry name" value="5-OXOPROLINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19278">
        <property type="entry name" value="Hydant_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="6">
        <location>
            <begin position="14"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="4">
        <location>
            <begin position="246"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Acetophenone carboxylase-like C-terminal" evidence="7">
        <location>
            <begin position="560"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase B/oxoprolinase" evidence="5">
        <location>
            <begin position="753"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1279"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="552"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1285"/>
            <end position="1300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4376062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1318" mass="142611" checksum="7D11DC9C4F6A8C61" modified="2023-11-08" version="1">MPFVEQPSQGAGIRIAIDRGGTFTDCLGIVPGKPDILVKLLSNDPTNYADAPTEGIRRILEQATGVSIPRGQKIDTSNIESIKMGTTVATNALLERNSTKCALVVTKGFRDLLRIGDQTRPKLFDLNIRRPETLFDDVLEIDERVTLHDSTEDKATLRDPKRDSYDLQKGVGGEAIRILRPIDSTSARQGLQALFDKGVRSLAITLINSYTFPNHELEIADIASDIGFSQISVSSQIFPMIRAISRGYSATADAYLTPLTRDYINGFREGFVGGLEDVNGARCEFMQSDGGLVGWQHFSGLKAILSGPAGGVVGFSKTCYDPLRKKPVIGFDMGGTSTDVSRYAGTLEHTFESITAGVTIQSPQLEIDTVAAGGGSILSYRNGLFLAGPESASAHPGPVAYRKGGPLTVTDANLVLGRLQAEYFPKIFGKNENEPLDYEGSKKAFGELLVKVNEDLKNTGGKTKTVEELALGFLDVANETMCRPIRSLTEAKGHRTSDHDLAVFGGAGGQHAGSIATNLGIDRVLVHRYSSILSAYGMALADVVRDVQEPCSALLSSSLEHLDERLTNLEKNAAEMLAKDGFAGQTIGFEHFLNLRYEGSDTTFMISRPDKGTWDDAFVAEHKRQFSFIMPGRDILVENVRVRATARSPSVEPEFALEQQLANAPPTAVSSKKISDQVQVYFEGGWCTAPLFHLSTLNQGDQVEGPAIILDNTQTIVVAPKSKATILERHVMIELDRSAGQANTEDIAPRAVDPVELSVMAHRFMSIAEQMGLALQKTSVSVNIKERLDFSCALFSPEGRLVANAPHVPVHLGSMEQAVMYQHKKYLGQLRPGDVIVANHPISGGTHLPDITCITPVFDDAGETIIFYTASRGHHQEIGGILPGSMPAGSVELHEEGAMIVSEYLVRDGVFDEELVTKVLLHDPAQYPGCSGTRKLADNINDLKAQVSANAKGAALVADLITERGLATVHLNMHAITANAEACVRDFMKRTYKETGGKPLQALDFMDDGTPIKLTITISPEDGSAHFDFTGTGEEGYHSFNAPQAIARSATLYVLRCLINQDIPLNEGCLRPLTFTIPEGSILNPSPYAAVCAGNPITSQRVTDVVIRAFEACAASQGDCNVFSFGINNDFDSDGKAIPNSGFGFGETICGGSGAGPGWNGTSGVHIHMTNTRITDPELLEKRYPVLLREFSIRDGSGGQGQWNGGNGIRRIYEFYRDMNASIVSERRVTRPYGMFGAEDGKSGVNYIVKKVGNRWCRLGGRKDFSVQKGDWFVIDTPGGGGWGAKDDEQTEGADRKGSAPVERRFVSQFQLAQEASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGF9</accession>
    <name>A0A9W8YGF9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001884</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGF9"/>
    <dbReference type="OrthoDB" id="3525185at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053178">
        <property type="entry name" value="Osmoadaptation_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38111:SF11">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38111">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="422"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="430"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="445" mass="49715" checksum="729757F8D8170D74" modified="2023-11-08" version="1">MTKTAKHGMQKRKNKALPKAKNQIAWAFQHSDNVVVNRISQRAMVTEAFYERFLSYFTSEGEGKDIQNRLTWLHRLPLISTDGTDDALVLALQATASAYCAVDSSNPALTRHAWNLYGDALRIHGRTVARSTSKHQVTLHMVSTSVLFSFFEAMQATNANAYRSHIFGAAKMLEVTGPGQCTQGILCQIFYHLRTQMAFVHLTGNGNETPVEVRKILHGTLEYTRLPMFQRLMSHIATLAEMYVEMRDGVINGTLHRPLNLGTYMAVKGEVEALWHEYTASANERNEQLKWEDPSSGAPMYRDAFTALTIAYFESARVLLAIVAPQLAASFVDLNDHYAAIMATAQYLQIHDIGCAYMRMATPLLLVALHSPKREQRRAAIYCFECWAGGSMRGISTLALQSIYRQRASKIPVDVRSAIADTPSTVSSDDSSRYSDTRTIETASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGY4</accession>
    <name>A0A9W8YGY4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGY4"/>
    <dbReference type="OrthoDB" id="5371646at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="193" mass="20428" checksum="367CEDD303C70FF5" modified="2023-11-08" version="1">MSDNNATKPAGGPKTSWTDREVLVYLWALVEKSGAKFDYNNTPRPGNRTVIAGQRKIARLATDLKDDLVALRSGQSGVDEAKTTPAARKKNGASGGAGSGSTARKRKAADGEDGDDDADAAPLRIFKRKRGPRKNKGNKGAAVEAEGDAKADGEGEGEGDVGDEHELEVKAEPMEDVELGFDEAEANFEVDGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH87</accession>
    <name>A0A9W8YH87_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">40S ribosomal protein S28</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RPS28A</name>
        <name evidence="4" type="ORF">N0V83_000707</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH87"/>
    <dbReference type="OrthoDB" id="10258930at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04457">
        <property type="entry name" value="S1_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000025">
        <property type="entry name" value="40S ribosomal protein S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000289">
        <property type="entry name" value="Ribosomal_eS28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028626">
        <property type="entry name" value="Ribosomal_eS28_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01200">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00961">
        <property type="entry name" value="RIBOSOMAL_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4377877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="68" mass="7726" checksum="F1F86D1DA05A0FB5" modified="2023-11-08" version="1">MDTAKAPVKLVKVTRVLGRTGSRGGVTQCRVEFMDDQTRSIIRNVKGPVRENDILCLLESEREARRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHB7</accession>
    <name>A0A9W8YHB7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000932</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHB7"/>
    <dbReference type="OrthoDB" id="3795464at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="147"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4378100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="210" mass="23941" checksum="55732B10DA698226" modified="2023-11-08" version="1">MSWYKDRGVPKGYSSIRQFRPTYRELKVLQRMGLIKNEGARGKLIVPNMLHPLVRSFYDKKPSPQSLNTKLTLIEAMPKSSMGQGVITKHAEPQLSTPGEARLSRTIVKSSLDPRLQTRFGSNSSFLTEGEQTEPILTSVAMPYNAQENAKTNNSTLEDKAETKLQELEDQVMKLHMDNIAKDREIRRLRAELAELRGEGYERGKKRPRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHC4</accession>
    <name>A0A9W8YHC4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Prion-inhibition and propagation HeLo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000348</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHC4"/>
    <dbReference type="OrthoDB" id="3779652at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040880454" description="Prion-inhibition and propagation HeLo domain-containing protein" evidence="2">
        <location>
            <begin position="29"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="488" mass="56160" checksum="F0C808AD8736C9BD" modified="2023-11-08" version="1">MAEVLGPVGFCFGCISFVLSTVPTAAKALDDFSQCVEYFQLYETRLVRSKARADKWNMYWRDKSDFGEHETVIERTKKDIDDLCQAIEDDISSNITDEKERKTWRHLKRGFRCGRFRKPRANEKTVSFIRSVGFVLFEKSHIEGWLTRLETAIDCIEKVSETVFAKRTAEHFNGSPSRAQVLETKELECFLQGITLLAQKLHEESSATTNGFEPETYGWALGLRPPAITVEHWKFIAEVNIEMRFSTRRSVDEENHFHLDVPFHKDDKRTHLDSEQIKSLVRRHTTSKGSPNTTNTAAKCSTQHPKARRTRPIGDLLKHAPKLFQDEAWRQDRATLVRGLSHWAILLWDTAWMEELCCYGLQIEKGVVGKDCISQMFNVGECPKYDRCHHGSRLRNLGIVLAQLVLGKLIRRTSQNDTGEYQQWVDGEWKVLYRSDIIAEVFVKTTSIPLSDAISFCLKDESALANDPFQPGFLFECIDHIYKPYVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHG5</accession>
    <name>A0A9W8YHG5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_000936</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHG5"/>
    <dbReference type="OrthoDB" id="5817083at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46283">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="438"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="517"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="10">
        <location>
            <begin position="73"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4378104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="567" mass="61803" checksum="7236F04B0FE83F24" modified="2023-11-08" version="1">MASLPPQRPASQRRASPPAESASSQQEQVARTNSFSAQSVDSQTLLLNSPPKQSAKPAVEAQKEAPAQSTTPEEDTEPRKCWICFNDETEDDETTSEWRSPCACSLVAHEKCLLDWIADMEAPSSRRRAGTRAGKILCPQCKSEIVVQRPRSYVVDGVRAVERLTGTLLIPGFALVTGTALYSTLTLSGMHTIYQIFGTEDALQILGPLYETPNPRVGSVAVRLVDHLRRHWRLDLGLPLIPTVLVASRTTFADSFLPFLPLIFFVSSGQPGDEMLQLQWPPSAAFSFAALPYLRGFYNAYYDRVCLPLEQQWLKEIQPRAGEDTPADVQAQGDDDHVHDDEGLDGGVVDGVEIEVDFDIFADWNNGGEADNHDAQENPPVPVARGPARPIDAPPLDNDAMPELIEINANAQEGAPAPNIPAQPAVPRRQRVRRERNIAFSTTSLADTILGALIFPSIAAAMGELLKHTLPSSWVTPPANAPATSWLGGWVHNNGGKAGGSVGKPTGFLQTRWGRSIVGGCLFVGLKDAVLLYVRWKMAQNHRRRKILDYDDKGKRKRGGTAAASSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHQ5</accession>
    <name>A0A9W8YHQ5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000371</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHQ5"/>
    <dbReference type="OrthoDB" id="443981at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14279">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058864">
        <property type="entry name" value="UBA_10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26286">
        <property type="entry name" value="UBA_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBA-like" evidence="2">
        <location>
            <begin position="242"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="365"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="414" mass="44508" checksum="319D4F4655CDED20" modified="2023-11-08" version="1">MDEDIRILEQEYCPPIDPGLISAIYFDYIGQENALGSARQLLDSLKATAAEEALTDFNPSGFSEAPDRDSPDKTSTDAESNADTWATQTATTDTSNLSNDISALGFGERSGTGSEASGEGGYFKDTEHFDVPTKELLLAETFPSLSLVKIAYTLKKCGNDFSKTTDELLNHVYFEDIRASPSEEGPIANGIDAFSEDHHVPQRGRKGKGKGKKKKQLDYSIETAYTSGSEASTAPPTNRWLNTTRDVEFITSRTSVSTKTVSSLYHTKGASLSATILELVRNNIKAHAKEGEPDAALVQDALTLNADFTTIDLEHTLALIRLAIPSTPNARDLAKALTTEQPAGETGGKGGGGIKLDLRYAPINLSDPTSSEPLKLPTLAPSARPRDLASTARARSEAFKTHLPPTAKGGRRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHW0</accession>
    <name>A0A9W8YHW0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7918 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000744</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHW0"/>
    <dbReference type="OrthoDB" id="5400327at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057678">
        <property type="entry name" value="DUF7918"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36223">
        <property type="entry name" value="BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36223:SF5">
        <property type="entry name" value="BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25534">
        <property type="entry name" value="DUF7918"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7918" evidence="2">
        <location>
            <begin position="251"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="410"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="470"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="568" mass="61646" checksum="F87D7E789DE4747A" modified="2023-11-08" version="1">MPCFLGLAVSIHTPQGPLAEYSIQKQSKFHRITSYIPVPPATIPPGATKPEQSTFAISITLLTPGLHVPYSTPKPTADDPNPKPKSVGGLPSYTGERGKYAPTIAPYKPLTTSPNETIAAYIYFDGRAKEEVATLLRRGEETWVNSRWVSVPESEGGGLAEREFLFREVGLERWLNGLDLEGKDKDVAAKIERRKQRLEKKRRKRREARDSSDEEDTKESGNGVLRYGESKDAPVETLSGNDEFFTTDSDSEDEPPMPEAAGQIKVALFRVLASGEIKRGEYSPQFDAHDDDEQNGGGEGEDIDHTTSFAKPKTLDPKSISTQTVTGIDPPDKPYAVFTFLYRGERQLRKMGILESEKAAKVTSPAARRKSGALDFGSLKPLNASTGTKNFGGYRDRSQTSPTKVKSEEAMDSDADDEDEIKVEDVDDDKEESGKGMLSPEDALKQGELAEGVRKIKLKRQHSAEPLGRPSASPAAESSASPLSGTPAPGASSATETSSSAAPKPSAELSNESSVASPFKKQRASLPGFDDNVRKSLGQALLSAQKERGNSEGNIPTIETKMEEDDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIA6</accession>
    <name>A0A9W8YIA6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amidase signature enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_000238</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIA6"/>
    <dbReference type="OrthoDB" id="167809at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00431">
        <property type="entry name" value="cysteine_hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050272">
        <property type="entry name" value="Isochorismatase-like_hydrls"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540:SF9">
        <property type="entry name" value="FAMILY HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G08700)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540">
        <property type="entry name" value="PEROXYUREIDOACRYLATE/UREIDOACRYLATE AMIDOHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="3">
        <location>
            <begin position="27"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Amidase" evidence="4">
        <location>
            <begin position="288"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4377413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="735" mass="79332" checksum="DE4A97F5BABE5C2E" modified="2023-11-08" version="1">MAKSMLSPSMLCFDAKPYPFKFPPERTAILIIDMQRDFLLAKGFGEIQGGNLEAVQASIAPTRKLLDACRNAGMTIVHTREGHKPDLSDCPSSKLVRQSAAPGNTQHKLVIGDKGELGRLLTRGEYGHDIVDELKPLPGEVVIDKPGKGSFWNTTILHQLKARAITHLIVSGVTTECCFATTIREANDRGFECCGIEEATSGYNDACFKKSTLDMIHWSQGLFGFIGSLQPLLDVLAPLSSRTLEVGSTPSQTPPAFDGDLTISSLQRAYKNGLSPVTVFDALYDKIESYKSIDPAVWIHLESRDKALDAARTLAARFPDRNTLPPLFGIPFSVKDSIDIAGIATTTACPPLAHVPSASAVVYDKVIAEGALFIGKANLDQLATGLVGCRSPYGITHSVYHEDYISGGSSSGSAVSVGANLVSFSLATDTAGSGRVPAGFNGIVGYKPTRGTISFRGVTPACLSLDCIALSTKTVSDARTIWQILEGHDPLDPYAKPEIGFERHINSIGPQSQTFKFGIPPPEALAICSIPTRRMFNETIAKLQKLGGVLTPIDWSPFQKAGQLLYDGTFVSERLASLPDDFLERNRSGLHPVIRQLMEAVVNRKSSAVDAYRDLQAKVLYTRQAEQVFAYSASGVDVVVVPTTPTHWRIDEVLADPIKKNSVLGEFTHCGNVLDLCGAAVPAGTYPVKELSGKEDDEGILPFSVTFLSGSRLDAEMLEIARRFEESVRAETVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIS6</accession>
    <name>A0A9W8YIS6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nuclear pore complex subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">NIC96</name>
        <name evidence="5" type="ORF">N0V83_000481</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleoporin interacting component (NIC) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIS6"/>
    <dbReference type="OrthoDB" id="203824at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007231">
        <property type="entry name" value="Nucleoporin_int_Nup93/Nic96"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11225:SF4">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11225">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP93 NUCLEOPORIN NUP93 DEAD EYE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04097">
        <property type="entry name" value="Nic96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="343"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="962"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="58"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="75"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="966"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="977"/>
            <end position="987"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4377653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1170" mass="127614" checksum="0E476008E86F19D6" modified="2023-11-08" version="1">MSTLFGNLGASQPASSGVTTGGLFGGAAGASSGPTPSLFGNLGGGASSAQSGTTQPASGGGLGGGLFSGLGGAKSTATTTPSLFSGTASTSQPQPQPQPQPQPQPQTQTGGGFSLFSKPGTQSTTTNPPPNQQQTTGLGQSSLFASTSTSTQQPHQQQQQQQQQQQGASLSQSNAGTTRSAHFDHLLERGRKRNAGQNGTSNFDELPSLQLGLGDIARKVRNLGAGGPSADQVRDGAQDRAAHYLLSASGVKMGSTLRDLNQFSTQAGIGATGPTQNLFDTDVDGYVSNLHSQSTLALIQEGLEQSKRDFDAFLEDNVQIEWDKQRQRIYEHFGLGRQSEEMAASQSTFGNTARGAFGRSTRKGRSMGPKGASATNKSTFGASAGGPPVLGASGSVLGGRDGPDRSVLGGQAGLQDRYVRDKQEKFMDEVKKLNDARLRENTFSVFHAFSDVEKAAGTEYSDQFIDAYGALISMTGERNELEASNPGSSNPKERSFAKDYLDDQSNSVASVRMRKRILDGSRKYLEKKFLEQVDEVLRRNPTEALVGGVPSLLNKIRGFVRAKVAFKELGADTEFFQRIGDTGEEFPWVIVFYLLRGGLLTEAAEYVREKRNFFQNTDRNFQAAITQYATDPDRRLSPETQQKVGHTHAQRVRINTPEDPYRTACYKIVGRCEMSKRNLDPIKQTMEDWVWLQFNLAREGNRAEENAGEIFGLEELRTTIGDIGQRHFMNEEAEAAGGYGVYFYLATLAGLFEPALKYLYDHNYVSAVHFAIALDYYGLLRVSDWTTAGGELLTYTTRQQAQLNFGRVVGAYTADFRAARADAAADYLVLICMNADLPGEAGKQQAGLCHEALRELVLETREFSTLLGDVKFNGQTTPGLIQERVPLLKINGENGLLNTITREAAKMADDNGRTNDAILLCHLAGEYDSVIGILNRALAEALSVELGQEPLRLEPLKPRLTGAELQQQQQQQQQQQASDPTSSSLSMLGTDDPVELAQKVLALYNEHSLWWRKVSQVNRDTIRILYQLNNAKKIIEARSYMEALDVNYLNPNFVDDTNAILQQIENLRILPLSARGNLSDIRSTANSFSSYPPEIARNIGNVLLWCIGCCSRHREHLLSGQFDDPMRKVLADQLLQKAKDLMVFAGLIRYKLPPRVFETLAREGQDVGAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YKW9</accession>
    <name>A0A9W8YKW9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphoacetylglucosamine Mutase</fullName>
            <ecNumber evidence="4">5.4.2.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">PCM1_2</name>
        <name evidence="4" type="ORF">N0V83_001000</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.3" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YKW9"/>
    <dbReference type="OrthoDB" id="1928at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004610">
        <property type="term" value="F:phosphoacetylglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.50:FF:000003">
        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049022">
        <property type="entry name" value="AMG1_III"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45955">
        <property type="entry name" value="PHOSPHOACETYLGLUCOSAMINE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45955:SF1">
        <property type="entry name" value="PHOSPHOACETYLGLUCOSAMINE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21404">
        <property type="entry name" value="AMG1_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoacetylglucosamine mutase AMG1" evidence="3">
        <location>
            <begin position="76"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="2">
        <location>
            <begin position="136"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4378167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="220" mass="23963" checksum="E637EF0BEBED3F25" modified="2023-11-08" version="1">MVITTSRSESSRPHTPTAQQQSMLRNLYASRSTAHQRASSISTTPRRNSTSASTSRPTATAPSSSRTTHSTPSRNTSPATPEKEALEVLRACINLINQSVGDALSDFLLVEVVLAHKRWGPQEWLSTYTDLPNRLLKVIVNDRRIFKTTDAERKLTSPEGVQSQIDKEVQKVRQGRSFARASGTEDAVRVYAEAATKAEAEDLARKVSEIVKAAGGCLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHM1</accession>
    <name>A0A9W9CHM1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Class E vacuolar protein-sorting machinery protein HSE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Class E vacuolar protein-sorting machinery protein hse1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">CDC25</name>
        <name evidence="16" type="ORF">N0V83_010585</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB).</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the ESCRT-0 complex composed of HSE1 and VPS27.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the STAM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHM1"/>
    <dbReference type="OrthoDB" id="546434at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11883">
        <property type="entry name" value="SH3_Sdc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056685">
        <property type="entry name" value="DUF7783"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056686">
        <property type="entry name" value="DUF7784"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057827">
        <property type="entry name" value="WW_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF368">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25006">
        <property type="entry name" value="DUF7783"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25008">
        <property type="entry name" value="DUF7784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23518">
        <property type="entry name" value="WW_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="16" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="8 10" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="10"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="15">
        <location>
            <begin position="724"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="14">
        <location>
            <begin position="893"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="71"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="151"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="623"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="96"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="624"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="656"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4362491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1146" mass="128261" checksum="A6C9B2E5A517EA84" modified="2023-11-08" version="1">MNGFNGTVSPGGMYVKALYDYDADDRTSLSFRQGDIIQVITQLESGWWDGVINGVRGWFPSNYCTVLQRPSEELEDDRNGGDLEDDHDTRSLGGTDYTNSDTESLSGNDTILPIERNQSSKEEEAAFWIPQATPDGRLFYFNTLTGVSTMELPLESPSPNENGPRDRVNVFIPDSSRPPPEMLASGYNVEDTDDETSASDFEGPGAKGSYSSRGRQRTSDGLSSATSMESMNAGFASRSYDTAGMSFSAAAIPPTGTTATSFANSPTIGGPNGAAAQSRKFYDDVNPVPLTWNRMVDDMRRAIERYRQAINNSDRSEFVKRAEDISDHLRLLLAAGSGTTDNHSGNPSIISTNKALYPHFRETMSRFSKLVLSSHIAASDFPPPDSYSKCLKEADGVLNGVYGFVEVVRQQRGEDIPRLVPGFVVGSEIGGSWHNNGTDARDSNPSMSFIDQEDYEPSIEPTVKLDSRVLERLEDIKRLIVSSLRRLDEQLVVRDRIITHQKHQAIGEHTCGAAGKVLDVCRPWISTIESINLASLTTTPQAQQLAEFSIQKQKAYDVVSELVIACQGVAAPLGDEWAELRGDSLEDRINAVRGVSRDLEATIAQQYAILHALSDAVPLADITPRTDSRRNTDSELTNSHTRAPSGAQRPLLKDMPQSQTYSEGTPLTTAEEKLIEPVRSANSNKKIKDFFGEVPVIPQTQVEEIPEFLKLDHEGEISYDHKVTPPQLRGGTLAGLVEQLTRHDRLDPAFNNTFLLTYRSFTTASELFEMLVKRWSIQPPHGLAKEDYQHWVDKKQKPIRFRVVNILKSWFDNYWMEGNDEEARILIQRVYNFAKDHVATTSTPGAAPLMTSVEQRSRGPDMPTKRLVPTLSAQTPQPILPKHMKKLKFLDIDATEFARQLTIIESRLYGKIRPTECLNKTWQKKLAPGEPDPAANVKALILHSNQLTNWVAQMILTQQDVKRRVIVIKHFVNVADKCRQSNNFSTLTSIISALGTAPIHRLNRTWSAVNQRSMATLESMRKLMGSTKNFAEYRDTLHRANPPCIPFFGVYLTDLTFIEDGIPSLIKRTNLINFAKRAKTAEVIRDIQQYQNVPYPLQPVPELQDYILTNMQSAGDVHEMYEMSLSVEPREREDEKIARYTPHYFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHN0</accession>
    <name>A0A9W9CHN0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Rad4-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_010053</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="300780at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000111">
        <property type="term" value="C:nucleotide-excision repair factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071942">
        <property type="term" value="C:XPC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2460:FF:000001">
        <property type="entry name" value="DNA repair protein Rad4 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.20.110">
        <property type="entry name" value="Rad4, beta-hairpin domain BHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2460">
        <property type="entry name" value="Rad4, beta-hairpin domain BHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.260.10">
        <property type="entry name" value="Transglutaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018327">
        <property type="entry name" value="BHD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004583">
        <property type="entry name" value="DNA_repair_Rad4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018325">
        <property type="entry name" value="Rad4/PNGase_transGLS-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018326">
        <property type="entry name" value="Rad4_beta-hairpin_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018328">
        <property type="entry name" value="Rad4_beta-hairpin_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042488">
        <property type="entry name" value="Rad4_BHD3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036985">
        <property type="entry name" value="Transglutaminase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135">
        <property type="entry name" value="DNA REPAIR PROTEIN XP-C / RAD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10403">
        <property type="entry name" value="BHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10404">
        <property type="entry name" value="BHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10405">
        <property type="entry name" value="BHD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03835">
        <property type="entry name" value="Rad4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01030">
        <property type="entry name" value="BHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01031">
        <property type="entry name" value="BHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01032">
        <property type="entry name" value="BHD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1177"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="8">
        <location>
            <begin position="646"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="9">
        <location>
            <begin position="705"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="10">
        <location>
            <begin position="775"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="210"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="421"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="892"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1262"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1416"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="219"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="259"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="461"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="477"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="892"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="922"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="993"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1006"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1022"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1039"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1080"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1141"/>
            <end position="1166"/>
        </location>
    </feature>
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        <location>
            <begin position="1292"/>
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        </location>
    </feature>
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        <location>
            <begin position="1319"/>
            <end position="1328"/>
        </location>
    </feature>
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        <location>
            <begin position="1345"/>
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        </location>
    </feature>
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        <location>
            <begin position="1370"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1432"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4362936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1441" mass="160654" checksum="68F097C648FD3261" modified="2023-11-08" version="1">MPPVLSRKRLQSDSSEPEPRPKRARAHNPPAGRSKQSVFETLDAPPKVKRTLSQTKAFLEQEDDESELSEPSSSDAEFEDVLPDSAKKTNGHDERDEESEDEDWEDALGVHHHAKPDHGPAPVISGDIALTLSAAPKTAFDSKPDGKKGPSKVQRQIRSVTHCMHVQYLMFHNLTRNAWIQDKEVQRTLVANLSYGCWREIEQYWRDAGISDGPSRAVATERKAQKTPAKETPNKKGRWKESGKLGVQVFDSPSRKRAVAREEKSKASKGGRKDLRKADRHARDWGATSEPLEPNTPNLSAGDPLLRLLRYLSAYWKSKFRITAPSLRKRGYLSPSTLEAEINAWRDDPLHPDVFGERVENLEAFREVARKCEGSRDVGHQLFTALVRGLGIEARMIVSLQPVGFGWSQSEEGKPKNLEKLKEATKSAQQTTPAKLLPVKAKSKPPTGIKRRRGTAGSDASESSELSSIISISSGSEEERPVKKSPKRRDYSEELPHPTYWTEAISNLTHTPISVSPLPRTLIASASSPDKLTDFYARGAAADKARQVLAYLIAFSSDGTAKEVTTRYLPKHQWPGRTKGFRMPIEKIPIHNKRGKVKRWEEWDWFKSLMRPYARPHNQRQPWDEVEDEGDLVPAQPEKKKDMHEEGGKETLQGYKSSAEYVLERHLRREEALKPGAKIVRHFASGKGDNEKSEPVYWRKDVVNCKTVESWHKEGREVKEGQQPLKYVPMRAVTVTRKREIEERERVEGGKVQQGLYSKAQTDWIIPDPIVDGRIPRNAFGNIDVYVPTMIPRGAVHIPLRGTARICRKLNIDHAEACTGFEFGKQRAVPVLTGVVVAAENENLVIDAWEVDEAEKARKEADKREKLVLSLWKKFLSGLRIVERMKAEYGDDAELPPKEPKRALEEKPKQKQSEWEVFQSHTDFEGGFLRDDAGPSDGGVVRQAPTHADNDEMGGGFFPASQEEPVHGDLIIDDDDQKTATRIPADTAYRTPISLTSALQQPGSETLEDDVDEQENEHVDSDENPEDDEDEESDLKLSSTRRKSAASRSSRGRGKKTPPKPPTRKRPSKLLDSSDEECSLSDIASNPPSTPPFSSSKRAVPQRKAARKSDAQVKSHFFANGSEGETDLTDMTDRNSPVKGGKRERGKEKGRGDGSGRGRGRGRGEGGEEGNVNKFTAMAPIILIVALIALPVILSSYLAYVGYTSYQRRRNSANDIERGVRDAERGVQLPHHRRRVSIGEHNTNGDGGGVVVVAGAGATGKTRMRLGRDVMTYEPPPEHQQERTYGQKGAAQDEEEFEDVDLYGDSAPPPPPKQHTNKTTSSTSSSSTPKCNYFTTPPIGTPKQQDGEEHPPWQMNEIRDSDSGIKDLTDNDDDDDDDDDDDVLERRVVVVVVGKQGRDGVEDGKTTRKGRIWRRSSDGGAFVKKKGPGGREAVDGRKSWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHZ5</accession>
    <name>A0A9W9CHZ5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010268</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="2331100at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052953">
        <property type="entry name" value="Ser-rich/MCO-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34883:SF19">
        <property type="entry name" value="EXTRACELLULAR SERINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34883">
        <property type="entry name" value="SERINE-RICH PROTEIN, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="187"/>
        </location>
    </feature>
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        <location>
            <begin position="289"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="397" mass="42217" checksum="8AFC50AC833D1220" modified="2023-11-08" version="1">MTGPDKQGFFSGFQPVDKVLDDPPKYTVTVNDTNPIFFYCSAPGSCITYSMVGAINPNSSTSIDTQHQLALNSTYMLNPGEPFPAESPLPSSNPASTALPGGNTGKKDLAAGAIAGIVIATLSVVVLAALLFFFWGKTKTLKDEVDRKESTVVRRVSPDSSTAMLEASRRNGGGGGGGGGGAGSDGLGIYQHYTPTATYFSPQEPQQQQQQQQLPAKFHLTSPTLAEHPAFSNAPYSPETITSPNANNVQRAISPLSHHTHSPTDFDLRPSLTSIPSTSDYYYPAHHNQLTPLLPPNPHSSRTISPPPQDAYKLELGPYGRQVTGNSNNEPAPSYFEAVHRPGPVHVNGRMGMGPVEMDGSSGVEGVQKGVKGGVVDAVVERPRWEEVSVDGEGKMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIC0</accession>
    <name>A0A9W9CIC0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009476</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIC0"/>
    <dbReference type="OrthoDB" id="5239630at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019034">
        <property type="entry name" value="UPF0390"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09495">
        <property type="entry name" value="DUF2462"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="85" mass="9009" checksum="02284C35778E8CE6" modified="2023-11-08" version="1">MAQGAVKKSKPVAARKPSNPGPKRGARVIKPKKASIISQNKIKHKSSAGLTAQTEKLLAQKAGHLEMLKGGKKDKKEGGDKKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIH1</accession>
    <name>A0A9W9CIH1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nucleoside phosphorylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_009642</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIH1"/>
    <dbReference type="OrthoDB" id="1577640at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000002">
        <property type="entry name" value="Dnaj homolog subfamily b member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053137">
        <property type="entry name" value="NLR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082:SF11">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082">
        <property type="entry name" value="ATP/GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="3">
        <location>
            <begin position="23"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Chaperone DnaJ C-terminal" evidence="4">
        <location>
            <begin position="760"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="394"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="735"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="411"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="422"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="525"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="562"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="738"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4364186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="847" mass="93490" checksum="D6515AF390D7350F" modified="2023-11-08" version="1">MANLLVSSSMNPRRRLQARDYTVGWICALPIELAAAQEMLDEVDEPIPDSLDPSVYTLGRIDVHNVVVACLPGGNTGTQSAATVAAWMKFRFTSIRFGLMVGIGGGVPSVESDIRLGDVVISQPYQQHGGVVQYDFGKTEASGHTIRTGWLNAPPPVLLSAIVEQRARHIRRQSDLEGYLAKFKRLETFNREAAGPDVLFQATYDHVGGATCNLCANEMKTRRPGRMGTGVGLHYGTIASGNQVMKDATRRDQISKELGGVMCFEMEAAGLMNDFPCLVIRGICDYADTHKNKTWQPYAAATAAACAREILSIVPGIPIPTVAKNMVDESVQSRIEMKLDQFMSQFMTELQLGYKTQAQFAQDLQKNLVDDDITEVDIEENRDIIDEWMKNTEDDGWLPELGPAEEKKPSNIGTSGVIDQSSSHDSKGIPQKDKSTHSKPNQAFCEDEESGNEDQWSDKNNDSSNAETEVPDEHHTPSPPPPPPVYRRASNPKLRSQDPETQSPAGGAWRPTSSAKNTKKPLGKSRSERERTQGRRETEIDDRYEESQGPSNARPKRRPHSTRPQSSREAVPNRPSSNPFIPRHQNSQSRDKPQYHGESPYPPIPSKHAEQPYPYNSCYSPGYNEQVEGHYHATPPLAHHPTNIPNAANGAWNPYNGHYDDPHGRYAPPPSGPAQPTVPRTGSYDQYADSRPPPPPAPNPTRRFHFSAGDQAGDGFNFSNADDIFSEFLRNSGGGTDFGSNNPATAKAQQSSDTEVTIVERPLPITLEELFNGTTKKMKITRKKYDRATGRQSTQDIVLEVPVKRGLKTGSKIKFADVGDQNPGVSQDLHFIVEEVCELVRDLQVCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIR5</accession>
    <name>A0A9W9CIR5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF155 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008416</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIR5"/>
    <dbReference type="OrthoDB" id="242766at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070131">
        <property type="term" value="P:positive regulation of mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003734">
        <property type="entry name" value="DUF155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051624">
        <property type="entry name" value="RMD1/Sad1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF1">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02582">
        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF155" evidence="3">
        <location>
            <begin position="127"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="419" mass="47012" checksum="E5E5E1A1F963C42A" modified="2023-11-08" version="1">MFPQRHDEVNVKAKDEISNGQQQADRKAPRKHAGKTSSLRSVAVEAQRSRAFVKSRGRTRFIDPDAETKTVTAYCAAETYSISAAARLVKAQGYELDPFQTGLYPQVIHVQTSDRPSEVKDYQQGDIFIFPSGTVVTWNVREREALKLVNQILPSAAEGSHLDLLETEDLDYLEDPSKETSEVVGDTIVLGTKVKQQEPPPDTLHDSSSSKPNNDTDQRHEVDTVLAKIAFSSGLARSTKLAVLETLLSNYQHSTRQIPTMLASNKTSALQSRGHRLFTRSFILRKTGELLSIRAQLNLYSELTDSMPDIFWDSRHELGLGEYYDQVGRALDVGVRIKVLNEKIGFAQEIASVLREQLSEKHGLRLEWAIIALIAVEVVLEFYRHWTEGKGRDDPASTEALLRRYLLTVAKEERPASHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIV1</accession>
    <name>A0A9W9CIV1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cut9-interacting protein scn1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">scn1</name>
        <name evidence="2" type="ORF">N0V83_009158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIV1"/>
    <dbReference type="OrthoDB" id="413993at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053044">
        <property type="entry name" value="Metallo-hydrolase/TatD-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47345">
        <property type="entry name" value="CUT9-INTERACTING PROTEIN SCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47345:SF1">
        <property type="entry name" value="CUT9-INTERACTING PROTEIN SCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="400" mass="45479" checksum="67BCE7F82AFEB3C9" modified="2023-11-08" version="1">MPAMSSDNKDQAFPWHLGVYDAHCHPTDTMASIEIIQKMKARMLTVMATRAEDQDLVTSTADKHSCRASVGIRGFSHQMYINDENAQDDGNSREQEQDVTLKGDAKVKHYQQVLQPSRSDPLSDEDRQIFLSLPDPTSFATFLQQTRKHLQKYPYALIGEIGLDRSFRIPEAWNAHLDLWSKRNANLTPGGREGRRLTPFRCSPQHQKKIFKLQLQLAAQMERAVSVHGVQAHGLVFEALSELWKGHEKEVLSKRERKKRGQDHPSVESAQLENGGAEKEDKPKPPYPPRICLHSYSGNASNFKQYLNPAIPTRIFASFSTAINLSDAMDEETPASFEEIVRTVPDDMVLVESDLHTAGDEMDRRLEDIIRRICKIKEWSLEEGVDRLGKNWKAFAFGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJQ0</accession>
    <name>A0A9W9CJQ0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJQ0"/>
    <dbReference type="OrthoDB" id="4850726at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF64">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="50"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="286" mass="33211" checksum="1047D018FF07D435" modified="2023-11-08" version="1">MAEEGLLVYRVLDPPGNAIRLLNLLPGQFSDDVYISIIHERLQDEPRTPYECLSYTWGSATQAHKKLYIWQKHTTASGEKDSLQYLNVRANLMTALQYLRLRDLPRVLWIDAVCIDQTNLTEKGQEVARMGQIYNKASRVVVWLGPEDESTPLALDIIQRISAGVELTWHHRGIHRTLPGSEAQVLEQRPEDSALSPEHWMALSRFLRRPWFSRLWIRQEVQLASEVRVLCGKREVDWGSLEKMSVFVEHKMDRKYIGVEEILLCRSLFRYLGSDRYVSPRSLPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK03</accession>
    <name>A0A9W9CK03_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008056</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK03"/>
    <dbReference type="OrthoDB" id="8026949at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051714">
        <property type="entry name" value="Znf_CCHC_NABP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23002">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="136"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="160"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="182"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="213"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="242"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="262"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="43"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="303"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="313"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4366420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="371" mass="38477" checksum="B95C1C6B6A7032DE" modified="2023-11-08" version="1">MARGTVASQLSSLPANPYPRAKEHVPQFTMCELCNRTIKTRDWSAHKNSKKHREAEAKEKEETEKPNDNSNGFGGDGSGFTIDTSNNNEFGGQDTFGAAPTNGGNDGWGSSGGFDNFNTSSYGNNTGGGGGGERACFGCGQTGHTKRECPTAGASGGQACYNCGQTGHQKRDCPSGSGGQTCYNCGEAGHRKSECTQPRKPMAGGGGGGDRVCFNCNNPGHNKADCPEPVASHGGGGGGRACHNCGQEGHMSRECPEPRVMKCRNCDEVGHVSKECPKPRDYSRVKCNNCQNFGHTAVRCREPKVETPGGDWGNNDNSGAAAGGWANAGADSAAAGSWGDTPQETTGNWADDSTAAAEGNSWGDAGTSGSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKF4</accession>
    <name>A0A9W9CKF4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor CWC2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Pre-mRNA-splicing factor cwc2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CWC2</name>
        <name evidence="20" type="ORF">N0V83_007468</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the first step of pre-mRNA splicing. Required for cell growth and cell cycle control. Plays a role in the levels of the U1, U4, U5 and U6 snRNAs and the maintenance of the U4/U6 snRNA complex. May provide the link between the 'nineteen complex' NTC spliceosome protein complex and the spliceosome through the U6 snRNA. Associates predominantly with U6 snRNAs in assembled active spliceosomes. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM CWC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKF4"/>
    <dbReference type="OrthoDB" id="10251848at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036002">
        <property type="term" value="F:pre-mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12360">
        <property type="entry name" value="RRM_cwf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000249">
        <property type="entry name" value="Pre-mRNA-splicing factor CWC2, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034181">
        <property type="entry name" value="Cwc2_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032297">
        <property type="entry name" value="Torus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF2">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16131">
        <property type="entry name" value="Torus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="8 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="19">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="18">
        <location>
            <begin position="187"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="16">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="354"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="362"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ4366938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="420" mass="45903" checksum="80254BD047740F92" modified="2023-11-08" version="1">MAEVQSPPAQTSLAPETSSDPSTSLEPSTSTELAPITTEQTIITTNAEGKKVKKIIRRKRRPARPQVDPATFKTEAPPATGTIFNIWFNKWSGGDREDKYLSKTAAQGRCNIAKDTGYTKADKTPGSYFCLFFARGICPKGVDCEYLHRLPTTTDIFPSNIDCFGRDKHSDYRDDMGGVGSFQRQNRTLYIGRIHVTDDIEEIVARHFAEWGQIERTRVLTARGVAFVTYLNEANSQFAKEAMAHQSLDHSEILNVRWATVDPNPAAAKREAARIEEQAAEAIRKALPAAYVAEIEGRDGGEAKKRRKVEGTFGLQGYEAPDDVWYAKEKGEWEAQREIEAGGMEGRLMLEAAADAQEGSDEGAVVPAQVQQEQQQRGGGGILSGSTLAALRGYTATPSNKRPAPVAGPLVGYGSDDDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL44</accession>
    <name>A0A9W9CL44_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006562</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL44"/>
    <dbReference type="OrthoDB" id="5343688at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="201"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="362" mass="39713" checksum="E00467D243ED4D1C" modified="2023-11-08" version="1">MRRQLPPSHLKVPPGPHAFPQRHCLGESTVIVNHYSGIEKSIPAIPSLPGFRTSAAFLTSLSANLRIDPDLPSPLGTPTTFRLNNFSHQPLPPTPLSFSTLPAPLSYPSPAETKIMSSTEATAPVKPPADELPQLTTYTAESEDNRIEGLRLVADSVAQQRQVSSKVLMVHPISLAGYLAIVAIAAQYMLRWYNDKMMVATTVGGITMSFLIFIRWMTGPYIGIAEDINLEWLGEDRLIVSKWGEDIIGALVLGWADNDAAKKRGRRRRGKAVVRAWTVKLKYRGKGVGEGLLEEAVKVAGERGADGIVFDQDHANSKRIMPAVFNGFLDKQEVKAEKALQKVADAKGNFRQRQGSPTWGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLK1</accession>
    <name>A0A9W9CLK1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ankyrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_006775</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLK1"/>
    <dbReference type="OrthoDB" id="4772757at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24188">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24188:SF29">
        <property type="entry name" value="GH09064P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="133"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="180"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4368418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="493" mass="55713" checksum="049C7CBAE9A89CC3" modified="2023-11-08" version="1">MADRDEIQNVETATSSVNKRDEPQQTTSPPSAQEFVPPQSDSDVLPLYSEEQPTTRDWLAELATTIRNETNSSLPVLAWPLAGDRHTYHEMEEFHQYRRIIKMYFEAIEIGNVGLVASFFGAGLVTTQTTNEAGGTPLLAAVEAEHTHMVRYLLDVGAAIDAYGVTATTVLHGKNNYPCTYRTPLQSAAQTGNLPIVRLLMGRGADDALIAPDGEVALRLAAGNGHREIVDYLPTRRGGGFKRWKTKHAEAMRRCKGAAVGIYWFGSVFVWYLPKFFLWTIPKELVVLPLVRGVKWLHEHRAEIPMLLVRTAKNVANGIKKFPARVWKVLKEIPRCIWDVFKECARFLWRIIKQLPHGIKTAFMLLWAGLKRIGLAVHNIVVRFTSFLHTICAAVLSFFQRITLRDVVDGFFYCIDAIIFDAPKKLWEWIYTLGKASLEALASVFWFWGFLARCMVEVVTYIPKKVWKMLVACGGSMKSGGQEVLVWFDPKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLS1</accession>
    <name>A0A9W9CLS1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA(Phe) 7-[(3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4)]-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.282</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_006219</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36635"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="Rhea" id="RHEA-COMP:10379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73543"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.1.1.282"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TYW3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.282" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLS1"/>
    <dbReference type="OrthoDB" id="263283at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1960.10">
        <property type="entry name" value="tRNA wybutosine-synthesizing-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003827">
        <property type="entry name" value="tRNA_yW-synthesising"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036602">
        <property type="entry name" value="tRNA_yW-synthesising-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418:SF1">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02676">
        <property type="entry name" value="TYW3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111278">
        <property type="entry name" value="SSo0622-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA wybutosine-synthesizing protein" evidence="10">
        <location>
            <begin position="8"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="256"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="260"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4369135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="309" mass="34039" checksum="782D9AA6D057ACEB" modified="2023-11-08" version="1">MASRFEARKQKILEQLEAPASEYHDLSPKGSVDEPIRDLISEINNLEGLVTTSSCSGRISVFLEGRKGVIEDAAPITGVEEPRAGPGGKGGGGAWLFISHTAVDVSERPSSPDYMSMFQFEKIVSEADDRPSVSSRYIHLKFEPMILHILSASVAHAQRILTAALTAGFRESGAVGLNSTKNGDTTPMVAVRSTGYSFDSIIGHHEEGGRNIPIVDANYLRTLVCIANERFKVNTERIARFRKALMESYYPQPALGAAKPDWEESDTRKARKREEGLARQRAFKKQKSEDRAVTDLESMSPDTLHGTFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM24</accession>
    <name>A0A9W9CM24_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Class II aldolase/adducin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM24"/>
    <dbReference type="OrthoDB" id="3238794at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000009">
        <property type="entry name" value="Class II aldolase/adducin N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051017">
        <property type="entry name" value="Aldolase-II_Adducin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004855">
        <property type="entry name" value="PRK06208.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672">
        <property type="entry name" value="ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672:SF25">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 14 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="1">
        <location>
            <begin position="46"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="278" mass="30494" checksum="C7B85997C8C4B026" modified="2023-11-08" version="1">MKLVVERESELENAPSPLEAMGHGGVPVRAVPKFDSYAAQRQWMLEHLAAAFRVFGREGYAEGIAGHISVRDPEFSDRFWINPLAVHFSMMTVSDLICLDMQGNVVGGNLSVPANAAGVSIHTACHLARPDAHAICHAHSLYGKAWATFGRELDMISQDVCYFYKAHSVYNNFGGIAFASDEGTNIAQALGKNKAAILVNHGLITVGQTVDEAAYLYTLLEKSCRIQLMVESTGLPKLIVPDEEAADTFRMASDPETLFVEFQPSYKLEEQLDPSFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM68</accession>
    <name>A0A9W9CM68_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoserine transaminase</fullName>
            <ecNumber evidence="4">2.6.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SER1</name>
        <name evidence="13" type="ORF">N0V83_005599</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>4-(phosphooxy)-L-threonine + 2-oxoglutarate = (R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:16573"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58452"/>
            <dbReference type="ChEBI" id="CHEBI:58538"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-serine + 2-oxoglutarate = 3-phosphooxypyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:14329"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.52" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM68"/>
    <dbReference type="OrthoDB" id="1703350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004648">
        <property type="term" value="F:O-phospho-L-serine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000082">
        <property type="entry name" value="Phosphoserine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000006">
        <property type="entry name" value="Phosphoserine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00160">
        <property type="entry name" value="SerC_aminotrans_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022278">
        <property type="entry name" value="Pser_aminoTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003764">
        <property type="entry name" value="PRK05355.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43247">
        <property type="entry name" value="PHOSPHOSERINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43247:SF1">
        <property type="entry name" value="PHOSPHOSERINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000525">
        <property type="entry name" value="SerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 13" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0718">Serine biosynthesis</keyword>
    <keyword evidence="7 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="12">
        <location>
            <begin position="8"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase class V" evidence="12">
        <location>
            <begin position="117"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4369835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="424" mass="46268" checksum="143656BE988C7AE8" modified="2023-11-08" version="1">MVNRSDVNYFGAGPAPLPTAVLEKASQVLLNYRDKGIGLCEISHRSPEANEILAEAKQALTTLLDIPDDYEILFLQSGGSGEFSATVYNLVSIWVEKKRAKIAQEVGDDKEEVLKRLRKVVDEELKLDYLVTGSWSLKASQEAARLVGAKHVNVAVDARKANDGKFGKIPSEDQWSLTPRASNPALVYFCDNETVDGVEFPSFPKSLEGGDAPLVCADMSSNFISRKVDVRKYAVIFGGAQKNIGNTGIAIVIMRKSLLPPHSTPASPDLLRELNLSVGPIVLDYPTIAKNNSLYNTLPIFDVWVAGQVMSGLVNSYGTKRIGGQEEVSNEKARLIYETLEKYSDVYRVVPDKSARSRMNICFRISGGDTDKEKAFLVGAEKKGLLGLKGHRSVGGIRASNYNAVSLDGAKLLVSYLEEYAKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM99</accession>
    <name>A0A9W9CM99_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_005465</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024338">
        <property type="entry name" value="MID1/Yam8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39142:SF1">
        <property type="entry name" value="AEL197CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39142">
        <property type="entry name" value="MID1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12929">
        <property type="entry name" value="Mid1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1324"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="4">
        <location>
            <begin position="264"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="818"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4369702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1348" mass="148803" checksum="DC1456773EE594AD" modified="2023-11-08" version="1">MRILEHFFPGHELNLQSLRTIAKAIKDGTFTAPPVQQTEGLFTNSQPSPDDGESVDDGEEQDVGELHEPLGCMMKDSRGKFRYVGAHSEIPFNAAVVTLGNQRKNPAIIPSPRVGLYPPSLPAPSPSTESGVEEPFYLPARELCDIYVSRFLEDVHCTYWLYPVESLLRHVDSTYSESAPSSSSSWMCSLYAMFAIGAANYVGFNGNSPPPGSPAAMDPKTSEDYIALAKQLIPTVYDEADIDSIRALAIMSIAMENMCSRVSAYLYMGASVQMAYTLGLHRDQLPENGTTIEREQNRRIWWTLFLLDQEISSRGGSPTVIDERYTKTLYPGLHTPLSWLATSVSLCRLKREIIQSVYTERSANSISFSTVSNSLLLLQKWHRQMPAHLIYDVPAPPTHKRAVAVLHLHYWGTTILLTRPFLLYLVIKYSALASSKKIWFERMGKTCIDAAQKSIAILQQMAADGTLSSLTAFDSTCILRLIMIFILAYAHTRTPQYSAHIETLMGLSKGMEQIGFTKMVTEETPGRLADLGIPEEPQDPNRNADAPVHLNDEMIAQLWGNWDPNFMTPLQTQQSLDLTFDDSGAFDINSEILAFTNLDESIIIDPSQVKMKTARAPLNTLIPSTLKPPLLQRPYLFLRTLKPVSWYNRDRRTLADLAAHLLRPSIPPCIPGISDKAVERAMPPLNLAPLQSRLLAYAVTTYLFLTLWTTFSPRPFAYAAESPVVLQHDRLSQPELHALPDGEFAPPGDEVADSEGIEGYTPEFALFDRSLVGRQAAEVSKLTNNEKTEMDMAAGATAYFVLEKTQSRVRRLTDDLLGARGGDNASGGVASGQEAEVASDRAADDEENNGELRKRQNGGRIWITANTCKQPTPSGNVTTPGDPPQLTMYVSTSSQNQKPGPGSTGNLATNSTGVLFDNGYASFDVQAGSDVYIGVAAPKFGGDWFGSWHFELAASTDGPYHSYNDTNPFLFMIDTDSESALFITYDLGTSNATSDVDKWKDNNPFHMYAFAAGDWTPITGMERSYCALKEQFYSNDTKNFTIDTSITTKFGGDLPNSQFHVQGLDTATTYNGFVVMEGGQEDLDIPGIGIVRAGGKVFQQFNWTTKADDSCQVLFDLDFCDTVAYAVPSSTKYKFNDTGLKALYDTQARSYYQNFSRSLAQVACDTTSEAQYSLAKTCADCESDYKNWLCSVLIPRCETWSADDDWLQPRNINTPFPNGTYTFDGNVTRGFNETHRDRFGYNQSRNPMIDQVINPGPYKEMLPCEDLCFDIVKSCPAKLGFACPNVGAARDMGYGKRDPQGDVLMCNFPGAVVKLNVQGAAGRVGFSVAKVVVVVWGVVAVVGVGCRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMT8</accession>
    <name>A0A9W9CMT8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_005260</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NmrA-type oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMT8"/>
    <dbReference type="OrthoDB" id="300709at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051164">
        <property type="entry name" value="NmrA-like_oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42748">
        <property type="entry name" value="NITROGEN METABOLITE REPRESSION PROTEIN NMRA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42748:SF28">
        <property type="entry name" value="NMRA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="3">
        <location>
            <begin position="302"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4370739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="623" mass="68500" checksum="F884013A003B929E" modified="2023-11-08" version="1">MGSIMQDERKVAIVTGATSGIGSWLAAHLHARGYRVALCGRREKEGQEVASSIDASGKSAMFVQCDVKSYESQANFFKTVWQTWGRLDVLIANAGCIDQGSVYNFARRDAAIDDLPAEPDTSCTDVDLKGTIYGTTLTTHFMRHNPQGKGGKIIITGSLLGIYACQTFPEYCAAKAATHQWAKTMGPILQRKENITINCVMPGGVDTAAMPHFSKAFRPEQMTLRSTLFSAYDTFLDDQSNTRTGELLEAAHDKLISWGHPGYKSGAFAKRTEAVFEPWFELMHGERSELPSAIQDWPDLDTKIIAVTGGTGSQGGGVVNIMKKTPGWKVRAITRNPGSDAAIKLAADGVEVVQASFDDEASLVKAFNGIHAVFAVTQWWEHIFQGKSQHEAGKLEEEQGMKIARAAASIRTLEHYMWSTTPSANRMMNGKLQAPHMDYKAHVDARIKSELPELAAITTYLYFGYYPQNMAFFPLCTPIEYPGSGGQYMQTLPTNPNVKVLLSGDMSVNPGIWVRQVLAARDKAYGKYANVALEKWTFQEMVDVWAEITGKKCVFTEISIEAATKLYGSVGNELALQFKFGEACDPWEDTEEHISPEELGIDGEEVVGFRGTIEGLKKAGFWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNE3</accession>
    <name>A0A9W9CNE3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_003325</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNE3"/>
    <dbReference type="OrthoDB" id="1919336at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051965">
        <property type="entry name" value="ChromReg_NeuronalGeneExpr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46040">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46040:SF3">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="5">
        <location>
            <begin position="114"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="3">
        <location>
            <begin position="114"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="70"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="199"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="223"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4373034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="761" mass="86117" checksum="9A1BF65AD6FE8ED4" modified="2023-11-08" version="1">MPDLSAILERLGLTTYLQVLRENGFHNWETVVDITEEDFATLNVKLGHRRALQREIASFRGVPPLLSLESESVSPEPASLSTLALETLTRQTSTPPPREKRRYRRHPRPDINAPKKPKTAYVNFADRIRTDSEISQLSFVKIAREVGRRWQELPAEQKRIWESSAARAMQEFEAQMDEYRKTDSWRRYQVYLRDFKMQQSMSTTGKRPGNSRATTDTSNNTRPYSATSPSSSDSPISPAFNASNEIGAEQCHNALTLAFSELVTLRGEILNQETRPYDEQHLPSEEVVRRSMYAFIRGTGSLLFMWTYEQADEILNRIYRPQERVDPMTLAECFTVAAMGAHYDMDCFSDQIRRVLYASGTLHFHEHTAGQDYLRTMRLLLSMSFYALLEKHMSARYLIAAGLQIARWKYPALHSSNEGGPNDNRKKILRSLIFMDCWLSYTLGYTSEATPSDILVATSLDSRDCLSVDQLIHMQTSKIGLIAAEITTTLASPKLVTKENISLLNQKLESWRVEVPDALQIPALTSSTPTHLTLYQRRAILMVHIMYLGALVLLYRQLLVAIAETQLADGSAYNPNFSTTDARRYRNDCAVAAQQIARILGLIDFDGTLTKRCWLIIYWAFSAAVVLLSSATTKLADGHTDGVDADLKYAKACMDKLEPSRQFEPIAARYLDTLWPLYDSLRDVLQRWVGRSKTSIFALIQADPSTMSPPIAVSKQEMGPISTKLTLLLTDPFGRKQGLPGDGSTRRLLNADGSCSVFWWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNU3</accession>
    <name>A0A9W9CNU3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyldiphosphate specific]</fullName>
            <ecNumber evidence="5">2.5.1.87</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">N0V83_004143</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>n isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate = a di-trans,poly-cis-polyprenyl diphosphate + n diphosphate</text>
            <dbReference type="Rhea" id="RHEA:53008"/>
            <dbReference type="Rhea" id="RHEA-COMP:19494"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:136960"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.87"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the UPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.87" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNU3"/>
    <dbReference type="OrthoDB" id="19639at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038887">
        <property type="entry name" value="Nus1/NgBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21528">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT NUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21528:SF0">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT NUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4372369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="346" mass="39169" checksum="F4702FF3F65A48D7" modified="2023-11-08" version="1">MAATGITPVQEAAFRKGTLNGKQLSAKEREELLKPFLPAEPTSSDVEAKAARRRRASQKSKVSKHGPVKNLLFFAVYHLIALLFSIFFRFRRAFRLVRGKLVSLLKYHHRTPEFIAHDVKNLEKLPKHLSVIVEYQEDDGSQGNAGLEGLVNDVCEIAAWAASAGIPFLSIYERTGVLKNYLPQTHASIWNTLESYFGPRRKPTLSLRAPHLSSYSPPNTPPQMAQPNGDNLKEERQHLTVLLLSEHDGRDTIVDLTRTLAEMAQKGDVRQEQINMDLIDAQLTDHVSSEPDLLILFSPTVQLKGYPPWQLRLTEIFHLPDNKGVNYQVFLRALYNFAKVEMRLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNU4</accession>
    <name>A0A9W9CNU4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002585</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNU4"/>
    <dbReference type="OrthoDB" id="2445133at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46424">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46424:SF1">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23187">
        <property type="entry name" value="UBX7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="224"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="437" mass="46550" checksum="0060D841682EBA18" modified="2023-11-08" version="1">MEMIQISNLLAQKAVLLRLQAGSTEAGFLAAFCDVTNTPTLVVIQNGKLELQLKSDVKKDEFTNSIRKVLGAEAIPGSSAAPTPPPQTSTPEAAHTRTTVEAGEDLYGASQTFEPARPAPPTPAIISTSSAKDKGKQKATSSPDPKPVPLAASVSSAQQDARDALRKKKRDDADELARIKARIEADKAERKAQAEARKAERARERDTNAQTEAQARTTTSTKGSQAKMVNLNVRLFDGRTIRSTFPRTATLQDDVRSWVDEEFAKLSADDPNINNARLPPYFFRHILAPQPSRELSAGDESQSLGDIDLAPSATLVLVPVKGYTEAYSGAGGGVVGTATGLIGGALGLVSSTVGFVGSTLGSVIGYGSSSTEAQTPGQTTESRLGHGTQRPASTEGSNIRVTTLADQRAREPRDQQFYNGNQLDTVPRKDDDHDKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPE8</accession>
    <name>A0A9W9CPE8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_003418</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRP18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPE8"/>
    <dbReference type="OrthoDB" id="10261918at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071021">
        <property type="term" value="C:U2-type post-spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.940.10:FF:000008">
        <property type="entry name" value="Related to potassium channel regulatory factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.940.10">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.110">
        <property type="entry name" value="Pre-mRNA processing factor 4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004098">
        <property type="entry name" value="Prp18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014906">
        <property type="entry name" value="PRP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036285">
        <property type="entry name" value="PRP4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039979">
        <property type="entry name" value="PRPF18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13007">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13007:SF19">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02840">
        <property type="entry name" value="Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08799">
        <property type="entry name" value="PRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00500">
        <property type="entry name" value="SFM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47938">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158230">
        <property type="entry name" value="PRP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA processing factor 4 (PRP4)-like" evidence="9">
        <location>
            <begin position="129"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="117"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4373127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="362" mass="42059" checksum="9F88177F3F766DED" modified="2023-11-08" version="1">MDFAKLMSAQIKKGKDTGSPADSQDKKYLKRSEIEAQRQAAYIAEQEAAERARRERIDNKRKVEEEEAEREGERREKRIRLAEESRRRREEEEEAEERRRRKRLGLPDLPPKKKEGEDEGTPIPAEEDIEDEDLVAKLRALDEPARLFGETHKQRLKRYKKRIGADKLAAIMTDGPIPTTLQLVPEKDMKVELKVPKNKEGREFLFRQLASYFTMVLKEWDVTLASRDAEVKESYQGKQAYAAMVQARENMRPLFKKLEKFDLPDSIVEPVVEIVHAAQERRYVDANDGYLRLSIGKAAWPIGVTMVGIHERSAREKLHESDKNAAHIMSDEMTRKYLQSIKRCLSFAQTRWPPTDQLQLMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPN0</accession>
    <name>A0A9W9CPN0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_002100</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 5"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPN0"/>
    <dbReference type="OrthoDB" id="431202at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000006">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022127">
        <property type="entry name" value="STIMATE/YPL162C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31735">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YPL162C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31735:SF1">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YPL162C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12400">
        <property type="entry name" value="STIMATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0697">Rotamase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040819584" description="peptidylprolyl isomerase" evidence="8">
        <location>
            <begin position="22"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="356"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="9">
        <location>
            <begin position="41"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="139"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="454"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="173"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4375021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="516" mass="55588" checksum="A3056CC0ECA33C80" modified="2023-11-08" version="1">MRLLFTLAALPLALLPSFASAQKLEIEVTRPVDCSRKTQNGDTLSMMYKGTLQSDGTKFDSSYDRGKPFKFTLGAGQVISGWDEGLLDMCIGEARKLTIPPDMAYGNSGSGPIPPGSTLIFETELMGIDGVKAEPVPARPTVAHPQETPDVEEAAPDMPTPEPTPTPSPVQLEPTTTAAAPAEPTDSASAQGSPLDGEDGNGECNLLGDFALIVQGALGLLAVSSLAVKRLRESPRRPIKIWFFDASKQVFGSVLLHLANVLMSMLSSGKFDVAATTSATPQYAGTDEKGDQPNPCSFYLLNLAIDTTIGIPILVLLLKILHAAFALTPLANPPESIRSGNYGQPPRATWWLKQSIIYFLGLIGMKLCVLAIFAFLPWIAWVGDWALRWTEGNTAVQIAFVMFIFPLIMNALQYWIIDNFIKDTDHGDGEGRYVVAAGDDSDDDADEEWLERQRRRREAGIDGDEDSDIEAAATEPLKEANPTAVPVRSNSAKGDEYDPATDGAGTSRAASKSRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPY8</accession>
    <name>A0A9W9CPY8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Low-affinity Zn(2+) transporter zrt2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ZRT2</name>
        <name evidence="10" type="ORF">N0V83_002904</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPY8"/>
    <dbReference type="OrthoDB" id="448280at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000007">
        <property type="term" value="F:low-affinity zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071578">
        <property type="term" value="P:zinc ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004698">
        <property type="entry name" value="Zn/Fe_permease_fun/pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00820">
        <property type="entry name" value="zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF69">
        <property type="entry name" value="ZINC-REGULATED TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="151"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4374163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="392" mass="42732" checksum="E55FD7FD2EE57406" modified="2023-11-08" version="1">MESVAIQVARLALRDAGDSPDTGNDAPVQCTSTNDYDGRMGVRISSIFVILIGSLFGAMFPVFARRAQSRLFPNWIFFFAKYFGSGVIVATAFIHLLAPANEALSNECLTGVIVAYPWPEGIALMTIFVMFFLELMTMRYAKFGEGDDHSHSHAGHSHAGHSHNTAVPSANSDDISLGDRKRTPEDSEALPTGNNPHLRGEDHLGHQRDHIANSEVAPDWQDRGVVPETYTAQITAVFILEFGVIFHSIFIGLTLAVAGDEFITLYIVLVFHQTFEGLGLGSRLADVPWPKQKRWTPYLLGLGYGISTPIAIAIGLGVRESFAPESKSTLLVNGCFDSISAGILIYTGLVELMAHEFMFSPYMQKGPVSRTLKAFALMTLGAGLMALLGYWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ31</accession>
    <name>A0A9W9CQ31_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosome quality control complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_001109</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NEMF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ31"/>
    <dbReference type="OrthoDB" id="207084at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.310.10:FF:000003">
        <property type="entry name" value="Zinc knuckle domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.310.10">
        <property type="entry name" value="ibrinogen binding protein from staphylococcus aureus domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021846">
        <property type="entry name" value="NFACT-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051608">
        <property type="entry name" value="RQC_Subunit_NEMF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239">
        <property type="entry name" value="NUCLEAR EXPORT MEDIATOR FACTOR NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239:SF6">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11923">
        <property type="entry name" value="NFACT-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05833">
        <property type="entry name" value="NFACT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="8">
        <location>
            <begin position="553"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="NFACT protein C-terminal" evidence="9">
        <location>
            <begin position="998"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="438"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="704"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1112"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="332"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="748"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="779"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="850"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="886"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="901"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="942"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1117"/>
            <end position="1131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00070414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4375832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1131" mass="125638" checksum="365F6506931E6B5B" modified="2023-11-08" version="1">MKQRFSSLDVKVIAHELAAQLTSLRVTNVYDLSSRIFLIKFHKPDHREQLLIDSGFRCHLTEYARTTAAAPSAFVAKLRKYLKTRRVTSVSQIGTDRILEFQFSDGLYRLYLEFYAGGNIVLTDADLNVLALLRNVDEGEEHEKLRVGLQYNLSLRQNYGGAPELTKERLRQGLQKAVDRQQEQPVVAGKKARKPGKDALRKALAVSITECPPLLVDHALHQASFDSTLKPEQVLADDDLLDKLVLVLQDARKITDDITKPDQIKGYILAKPNPSAPAAGEEASDKSKYLYDDFHPFRPQQFENSDYTFLSFDGFNKAVDEFFSSIEGQKLESRLTEREQQAKKKLEKARKEHEDRIGGLQQVQELNFRKAETILANVHRVTEATEAVNGLIRQGMDWVDIARLIEREQNQGNAVAQLIRLPLKLHENTITLLLDETNWDQGQEEDDEGNETSSVSEDTEDEDDKPRKKTPTTKVAPQPQLAIDIDLGLSAWANSTEYFDEKKTAANKEERTVQASTRALKSHEKKVAEDLKKGLKQEKEVLRPVRKQQWFEKYIYFVSSDGHLVLGGKDAQQNEIIYRRYLRKGDVYVHADLKGAVPMVIKNKPNNPDAPIPPSTLSQAGHFSVCTSEAWDSKAVMSAWWVRGDQVSKTGQTGEFLPAGMFNIKGRKEFLPPAQLVVGLAVMFEISEQSKANHHKHRIQETAVSAADMATEPTEDTKAAEAVQSEPSDDEDDFPDAKINSDSEEDFPDAKINHTDESDAESEAAAPRSNPLQSRNTEMQEDSDEEDNNYPTNEDSEFVMSGGKNGLSQKDDTQDDAGSMADTERTQGTQGRRHLSAKERRLLKKGQLPGSISASSEPTSVATDGNGDESASAEEDIQAAGKPPATVTSQASKATSGPVPRGKRGKAKKLASKYAAQDEEDRTLAMRLLGSKSGQQAAEVAAQEKRQKEEQAQADKQRRRDQHLRAQAAGKAAEEARRAALENAAQEDDDEGDEVLKANLQNLDAFTGRPLPGDDLLSAIPICAPWSALSAYKYKAKIQPGSTKRGKAVKEVLTIWDHAGKDAKALDKSSQDVERIWPKEIELIRNWKETEVVGVVPVSKVRVMIAGGRRGAEIAKGKKKSARGGRGSKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQB3</accession>
    <name>A0A9W9CQB3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GTP cyclohydrolase II</fullName>
            <ecNumber evidence="3">3.5.4.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RIB1</name>
        <name evidence="11" type="ORF">N0V83_001353</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + formate + 2 phosphate + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:23704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58614"/>
            <dbReference type="EC" id="3.5.4.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP cyclohydrolase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.25" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQB3"/>
    <dbReference type="OrthoDB" id="5569761at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003935">
        <property type="term" value="F:GTP cyclohydrolase II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00641">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10990">
        <property type="entry name" value="GTP cyclohydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032677">
        <property type="entry name" value="GTP_cyclohydro_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000926">
        <property type="entry name" value="RibA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036144">
        <property type="entry name" value="RibA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001591">
        <property type="entry name" value="PRK00393.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327">
        <property type="entry name" value="GTP CYCLOHYDROLASE II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327:SF29">
        <property type="entry name" value="GTP CYCLOHYDROLASE-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00925">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142695">
        <property type="entry name" value="RibA-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="domain" description="GTP cyclohydrolase II" evidence="10">
        <location>
            <begin position="228"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="181"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="204"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4376072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="399" mass="43788" checksum="3B7F51E1A31B66BA" modified="2023-11-08" version="1">MPELPAHASPDLPPLYSPHPTSPGRDGFDFQTSAQGVEVEAHDTLARTKSHDPPASMVSPAFTPPATPGVSTPSHPRPPRSVPVDTSLHSDSPRPELLQKLPEVECEVRARIPTTTGHEMWLHVYRNSVDTKEHLAIVFGNQIRSRSLDAERPGETELDRMTRGAYVGRLYPGRTNSRVEQLKRAEGVPTKRRPESEFPQSEKSNGLLSPSSASSESGDVQTISSSELPLVRIHSECYTGETVWSARCDCGEQLDEAARLMADPILSPSGGAIIYLRQEGRGIGLGEKLKAYNLQDLGNDTYEANILLRHPADARSYGLATAMLMDLGLEGERGIRLLTNNPDKVRAVEGPGREVVVRERVAMVPLAWKTGGAQGIQSEEVEKYLSTKIEKFKHMLDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQU8</accession>
    <name>A0A9W9CQU8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_001034</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQU8"/>
    <dbReference type="OrthoDB" id="424974at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF87">
        <property type="entry name" value="COL-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="29"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="66"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4375758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="719" mass="81870" checksum="6BE103F9C079BF20" modified="2023-11-08" version="1">MEASNNDRRDKADEGHANKRRKRGRQPKSCVACHRRKQKCDGELPCFNCARRDVASSCAYSDEAEAHRRQTSIAPQVNQQGDRPPSPMNEQNSDPPGSAADPTAPRHPEQHRSSGRASPEPRDAQVGNLFRSRDAPSFFGSSYFGPQAAAKIIEAPAPDLSSGIRHAAASTHSFRDVGGPFSQIWDLLGLLPRNKASVDRLVEVFLAELNWTIDAVHPASFKAKYDEFWERKFGFDDFASMDLRWLALLFIVLAYGVFLDCPFPKNTEVQRELQETSLRFYWASRRAIVIAPTFYGESTDLVRAGVLVTRYLIHLRRVPEAWLTTSFAMRMAQAQGIHIDGERWRLPRKETETRRRLWSHLYMLDKTIALAIGRPFAIVDQQCLVKRATNVWLDDEPDDRAATVPELPLSEPTLSVYNFLAHDLAKIVGNIQERCFGLFTVSYDTVLALDHDILEWEAKLPDYYHLKEPDTSRDQLHPFLYWNRLHLHSMYHFARVTLHRPYLLRQSITNRFRHSHDACISSACADLAMRIDYFQQPLHDRLKWTLGPHHLFNSALVLGIIAVKDPHSRRCHAILEDLTAYCDMQRADIWLNEFALAEVKIIELCIKKATQFRANRAMSTVEPPTERASQDLRYEQLQAPEAIAANGSNNNMNTFTSPNTELSSDSIPGLPHLAAGGGPFQWQAPWGDPAFPLFEPTDLQQWEHVLDSITQDQIMFGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRG1</accession>
    <name>A0A9W9CRG1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Intradiol ring-cleavage dioxygenases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001657</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRG1"/>
    <dbReference type="OrthoDB" id="121380at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03457">
        <property type="entry name" value="intradiol_dioxygenase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.130.10">
        <property type="entry name" value="Aromatic compound dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015889">
        <property type="entry name" value="Intradiol_dOase_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34315">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34315:SF1">
        <property type="entry name" value="INTRADIOL RING-CLEAVAGE DIOXYGENASES DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49482">
        <property type="entry name" value="Aromatic compound dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040741928" description="Intradiol ring-cleavage dioxygenases domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4376374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="373" mass="39473" checksum="F3BB19F36F1EB75B" modified="2023-11-08" version="1">MVYFSSAAVAVVAAFFANQAFAHPGHDIREEMAERAAFLSSAEYTNLDHCAEQLEARSTEMIARRKAMVDHLRTKRGIVKRDFDTVLATNHHSNASVTPNSPSDVIFAGNSSCILQAEATEGPYWVSGEYVRKDITNGLPGVPFTFDVQLIDTTTCQPLNRIALEAWHCNSTGVYGGVIAGGNGNQADATNINNTMFRGIQFSNEDGVLQFDTTFPGHYTGRTTHIHVLAHVNSTLNPANSTLTGGHVSHVGQLFFDQSLIHEVEAVAPYSTNTQSLMANSEDGIFAQEAATSDPVLNYVYLGDKVADGLFGWVTVGINPNSVTTPRAAASLDQNGGHANAGGGFGPGGPGGPWPTGSGAPPFPFPTSTGTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRU6</accession>
    <name>A0A9W9CRU6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_000159</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRU6"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050775">
        <property type="entry name" value="FAD-binding_Monooxygenases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43098:SF5">
        <property type="entry name" value="DUAL-FUNCTIONAL MONOOXYGENASE_METHYLTRANSFERASE PSOF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43098">
        <property type="entry name" value="L-ORNITHINE N(5)-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00370">
        <property type="entry name" value="FMOXYGENASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4377334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="558" mass="63141" checksum="177E11C07ADD7C54" modified="2023-11-08" version="1">MGSILQETQQPYDVLIIGAGLSGLCSLYHIRERFPTWRVKVLEAGTDVGGVWYWNRYPGCQIDTESLSYCFSFDKELLQEWEWTDTFAKQPETHRYIKRVAEKHNMEKDIIFNTRIKSAHWDDRSHTWTFVDEDGDEYLTRFFVSCLGFLSSPTLPKIPGIADFKGDSFHTSRWPADLDVGRDFAGKRIGVIGTGATGIQTITALSKAPSIKSLSVFQRTANWSAPLRNVPLSVEDMAKAKGEYDAIFQRCATTPTCFLHEADARKSLEVTPEERLEHWEKLYNSSGFGKWLGVFSDTYVDREANSMYSNFMAEKMRQRVRDPLVAESLIPKNHGFGTRRVPLESGYFETFNMDHVHLVDLQKTPIARVVSEGIETEDGKLHELDVLIFATGFDAITGAFSAIDWHAKDDRPLIASRGSERYSSAIWPDHRPKTYLGLSAPAMPNMLMVLGPHQPFGNATRSIEHASGVICDIFAYCKDNGYTYFEPTIEAVDSWTEHVFKCGSTGLVNEVDSWMTGINKNVEGKTERTVARYAGSAIEFRKRCERCKESGYEGFVFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CS95</accession>
    <name>A0A9W9CS95_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CS95"/>
    <dbReference type="OrthoDB" id="3796132at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="166" mass="19153" checksum="6AB659146A4A15D6" modified="2023-11-08" version="1">MFCPLLSQIQTLPLADITILAEQSLHPAILHTFCRAHGQVITTGNMAPLTELIYYQLGSNLVAQEEWNEMTRLQLEYELILRGLLEISEVRTEWQLRLRLAAAVLMEKKMAENDLQEDVETEGHEDQVENEEEGENMQIEGNDDDDEDDRDWKVIFTKSNGQTMVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSC0</accession>
    <name>A0A9W9CSC0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000808</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSC0"/>
    <dbReference type="OrthoDB" id="3690267at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="303"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="382" mass="41352" checksum="B4BBF0C9B6EE8D2A" modified="2023-11-08" version="1">MSNDTINNKMKGLKSHKKAQKAKQLAKRAAAAEKSHPKKTAGSVIVANEQTTLSLATLGPEKAHVGTATKIEKHLPAAVIETEVVAGAEFDQTTASKEVTVNINAHRKALEVALYDNMETPMQMLARLNFAVTADSEFHPDDEENQPAVDELNAALTTDGRRAIDKHYSFELCYTVPRKNLLTAHALELAKSQSALSTDAPEHGDFEVHLPVDQLFSMVSSTGAKVVGKSKPAVARTLESVEPQQMAERNPVPTATSTTPRGLGISIDQLLSAAARTTDGILRAPISNTQTSLIHKTPMGIDSSSNSTSSSGPHSRSISPTEHGTHTPPTHYSASPPPEYSYGPEQCFSANLYLFYHQEVHNRLLMAPPPYHAYGWRPYGYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSD2</accession>
    <name>A0A9W9CSD2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000807</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSD2"/>
    <dbReference type="OrthoDB" id="27483at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="194" mass="21707" checksum="94960CD64C875B49" modified="2023-11-08" version="1">MAGNSVTMDVKSIPLFQYVEEALQRFDFMPHDGARTLPQTLKGSDMAVYDVFKAVGLELQVRAVLDTRNSLNEYIADYEERNMYNSEDEVRSDYEPPRLQTHTVVGKLGGYGTTETGGWEGDGLDEVIEEWSESFMNIVWVNEPHHSSLDMVHLTYGNEAGIGTLYSFAALLVTIPPPQERGLQGMQNNPIAVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XX95</accession>
    <name>A0A9W8XX95_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_010955</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XX95"/>
    <dbReference type="OrthoDB" id="444432at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024747">
        <property type="entry name" value="Pyridox_Oxase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34071">
        <property type="entry name" value="5-NITROIMIDAZOLE ANTIBIOTICS RESISTANCE PROTEIN, NIMA-FAMILY-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34071:SF2">
        <property type="entry name" value="FLAVIN-NUCLEOTIDE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12900">
        <property type="entry name" value="Pyridox_ox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4362014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="190" mass="20529" checksum="753E4EB260FCF11A" modified="2023-11-08" version="1">MASPSYPKTSVNKLGRLANRVHALINQCPILHVSFNDPEHTFPVVLPMLGCTGNFGEQDADASSSEQDVYIHGYVSGRVFKSGKEAGEQGLPITIAASLVTDKTEALYAMQKITDNLLPQRWAKSRTPPTPAELTSTSILRVKIASASAKIRVGGPSEDRADLKDKEVGETGRAKAYAFEAVEMDGKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXT6</accession>
    <name>A0A9W8XXT6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Integral membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_010812</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM234 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXT6"/>
    <dbReference type="OrthoDB" id="43458at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018908">
        <property type="entry name" value="TMEM234"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28668">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28668:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10639">
        <property type="entry name" value="TMEM234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4361871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="16821" checksum="D46DF4E65350F1A1" modified="2023-11-08" version="1">MDPDAPVQSEPQPSVFRWILGFLMVGACWGLTTPFMRKAAMNYSPPQRPSLTDPNNSWLKKKVLGVFYAVIGVLSRPAYAIPLLLNVTGSVWFFILIGKAELSLTVPITNSLAFLFTVLGEWWAEKKVISRDTWIGMAFVLAGIGLCVQSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY09</accession>
    <name>A0A9W8XY09_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1996 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009996</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY09"/>
    <dbReference type="OrthoDB" id="74764at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018535">
        <property type="entry name" value="DUF1996"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43662">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43662:SF5">
        <property type="entry name" value="DUF1996 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09362">
        <property type="entry name" value="DUF1996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040736134" description="DUF1996 domain-containing protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1996" evidence="2">
        <location>
            <begin position="31"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="334" mass="36056" checksum="9DEB8CDEB2249E0E" modified="2023-11-08" version="1">MKALSAALALIAPVQAGLRFPCSTLTFQRLDPAGQPGSLPSAHVHHIVGGNAFNATMTGDVSSRATCTTCQMADDFSNYWTATLYFKHPTNGSYHRVAPMPVQPLLGGSDGAKGGLTVYYTQFDLSKDNLNQNPIKAFPPGFRMTVGSPTATSKDHAGLSYQCMTGGNRGAITDAMPTKPCTGGIFTTHHFPACWDGKNLDSPDHQSHMYNTVTSEGFTNAPKCPSTHPVRVPQVTFETVWDTTKFNSMWTSGAPNPFVWSFEGAASGYGTHADYMFGWKGDSLQKAMDKSECFYDGCGSITKQKMAVANQCTVKDFVNEPTEGWLKELPGMAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYF0</accession>
    <name>A0A9W8XYF0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mannan endo-1,4-beta-mannosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010164</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYF0"/>
    <dbReference type="OrthoDB" id="5286354at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006080">
        <property type="term" value="P:substituted mannan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04086">
        <property type="entry name" value="CBM35_mannanase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005084">
        <property type="entry name" value="CBM6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022790">
        <property type="entry name" value="GH26_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000805">
        <property type="entry name" value="Glyco_hydro_26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079:SF4">
        <property type="entry name" value="GH26 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079">
        <property type="entry name" value="MANNAN ENDO-1,4-BETA-MANNOSIDASE E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16990">
        <property type="entry name" value="CBM_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02156">
        <property type="entry name" value="Glyco_hydro_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00739">
        <property type="entry name" value="GLHYDRLASE26"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51175">
        <property type="entry name" value="CBM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51764">
        <property type="entry name" value="GH26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040867258" description="Mannan endo-1,4-beta-mannosidase" evidence="5">
        <location>
            <begin position="22"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="CBM6" evidence="6">
        <location>
            <begin position="24"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="GH26" evidence="7">
        <location>
            <begin position="147"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4363046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="452" mass="49045" checksum="A2080289F02A5E22" modified="2023-11-08" version="1">MFMACGIVAIAAALLGSTATAQTITLQAENATLSGVTVGTSVAGYTGSGYVEGFDEAADKITFSVSVNASSLYDLSIIYNGPYGDKYTQVVLNSAGGGQVLLNTGSNTIEIQNNWGWYLIDAITLSPSAKRPAHNIDTTPVTKNASADARALLKYLGSIYGKKILSGQQDQASLDWVTANVGKTPAILGVDLMDYTESRISRGASSTDVDKALAFATQKGGFVTFVWHWGAPTGLYDNSSQPWYSGFYTAATDFDVEAALADTSNANYTLLMKDIDTIAVQLKRLRDAGVPVLWRPLHEAEGAWFWWGAKGPEACKKLYRILYERLTGYHGLNNLVWVWNSVKPEWYPGADIVDIVSADTYTQGDHGPISATYNNLLSLTNDTKMIAAAEIGSVMEPSQLQAYQADWVYFCVWSGDYISGGSWNDLDLLKRVYGDEYVLTLDEVQGWRNGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ90</accession>
    <name>A0A9W8XZ90_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010702</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ90"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00413">
        <property type="entry name" value="Glyco_hydrolase_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38121:SF2">
        <property type="entry name" value="ACYLTRANSFERASE 3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38121">
        <property type="entry name" value="GH16 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="288"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="2">
        <location>
            <begin position="37"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4361762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="527" mass="57636" checksum="EEA21A18D140C374" modified="2023-11-08" version="1">MRPHPYEKNWNARYRAGASPSNIGNNESSAPGWDGNAWTMSIDRPTKNHAVVGASIRSLRRDILYGTFESVLSPPKAGSGGTVLAMRLDYNESQTLNLNVMNADAPKDAWTSFMIYGDWRGERSKGVNFTDFGNSSYNFATSPWGFVPYRIDWTDKKADFFIGDALARSIKSHESPKEWPRTPSTLYIRHSSIGDAYTSEGPPPNGSYAQLGRIRAFFNSSMMTTAEHASFDARCRGLEASDNAPAANSSSTSRQCLVSDMSLRGSTTYSTTATTQFKQRSIDYTKRWPAIFMASTCIAISTILLLHALFKRAPWSKKKTEVPTEATADSARASKEYYVTPSMIFSERDGLALAAQFPGTGTPGAVTPGTQTPGHRSLRYSDFSPPWSEVEGPYFTRSVSSTSLAAPARSRPESGSLHIPPLPVGLSIQQRSRCEDITAILTAKDSNQPTANVRGSLDISPVEPNRQISAFVELIDEKKDPEVTVKEIDIPSAATESAKPAITPPKQRVDYLAGLVSLPILPFSDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZN1</accession>
    <name>A0A9W8XZN1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_010558</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZN1"/>
    <dbReference type="OrthoDB" id="61110at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF3">
        <property type="entry name" value="F-BOX PROTEIN POF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="5"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="84"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="605"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="605"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4362465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="636" mass="71898" checksum="F9308D89ABA6F8F0" modified="2023-11-08" version="1">MTDRISSITDLPLDILVLVFPYLDAKSFLSLCSTCKAFQQPSIRLDPAYWSFATRSTFRVPNQPVVQHDGVRWQKMYRRLLTQSRVFTWGSNSHSRLGHSYEERIHHNPTMPRIMRPGRMRFNNHCSVPTEMEDTRNLGVIADMQCGGWSTTLLTSKGSLHSVGVLDGQRILAASERLQSLKFPEGYPASSAEAQYEEPTIAIRRFSAGRSHILALSDSGRIWSWYHVKRPALQVRFANLDLKELSLHASSRQTSTYGQVKEVVAGWSRSSAFVHGIGIVVWDPVERDHGDDATDTMLVLEHAEVPRTGYQHIPGHNESEEEKTLGAEIGTVKSYIVLEHFVVFVTDIGKVFCGRFGDKNRVDDVLELRQLRTEKGAPLDVQGSFRRFAVFKNDDVITTDQDYLEACWNTRSDSEQANIQGLRKIPALQHNDVISVAFGDYHYLALHSNGKITSYGTEMQACGALGLGDEQTSGGIARGVVYDRFSHNGQLLPHAYTRGRQVWFDARKKDWLEYVVSGHKDQEESRERRSLFTNANNVQGEVSEWFEQEGRAWDQDGGDDGLGAYFALRVSAAGWHSGALVLVNEELASKEPTYKYEDENFPRLRLADGTEMPGEKDFDEWREGRPEWQRNEGSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0N4</accession>
    <name>A0A9W8Y0N4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010442</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Cyclase 1 superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0N4"/>
    <dbReference type="OrthoDB" id="5396at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.50">
        <property type="entry name" value="Putative cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007325">
        <property type="entry name" value="KFase/CYL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037175">
        <property type="entry name" value="KFase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34861">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34861:SF10">
        <property type="entry name" value="CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04199">
        <property type="entry name" value="Cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102198">
        <property type="entry name" value="Putative cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="39104" checksum="86D330C9A6904AAD" modified="2023-11-08" version="1">MANTLKTAQNRLSALAQTLTTGGGSEYSFPAFDDLPKVEGMPQGNLWGFFDKDGKKDEVGTINLLTPAVVQGASREISTGEHIQLDWPLHNVQYPGFNRKEFHQKKINFADFSDFTAMDDEVYINTQAGSQWDSLKHFAHQASGTYYNGLTHEQASHADTNGTHNWCQRGGIVGRGVLCDWLSWYEAKKGEAPSPVTRHEIPVDEIIETLKWQGTSLRQGDIMIIRSGYVRWHNNASETDRKSGTRDNSVAIGLQANEKTIRWLYDQHFAAIVGDTVAFEAWPPKFTDGWCLHEWLLVHWGTAIGEMWDLERLSEKCKEMRRYTFFLTSAPLHVKGGIGSPPGAIAIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y102</accession>
    <name>A0A9W8Y102_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pentatricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_008738</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Regulates mitochondrial small subunit maturation by controlling 15S rRNA 5'-end processing. Localizes to the 5' precursor of the 15S rRNA in a position that is subsequently occupied by mS47 in the mature yeast mtSSU. Uses structure and sequence-specific RNA recognition, binding to a single-stranded region of the precursor and specifically recognizing bases -6 to -1. The exchange of Ccm1 for mS47 is coupled to the irreversible removal of precursor rRNA that is accompanied by conformational changes of the mitoribosomal proteins uS5m and mS26. These conformational changes signal completion of 5'-end rRNA processing through protection of the mature 5'-end of the 15S rRNA and stabilization of mS47. The removal of the 5' precursor together with the dissociation of Ccm1 may be catalyzed by the 5'-3' exoribonuclease Pet127. Involved in the specific removal of group I introns in mitochondrial encoded transcripts.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Binds to mitochondrial small subunit 15S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y102"/>
    <dbReference type="OrthoDB" id="1908178at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936:SF1">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN GUN1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936">
        <property type="entry name" value="PPR_LONG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="5">
        <location>
            <begin position="361"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="77"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4365120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="596" mass="67723" checksum="6014E3CE2C064D5E" modified="2023-11-08" version="1">MSLLRTLDRTSAVSNTAHARPFLGFLYPSLESKTSKRSLSKRAINRSPQLTAPGACMDNLFIHALVRAGSCREHATQLSTRPRVAPISSSHHKPRRKATWPEHDESEVKIGVGPERPFRKVRNFAENELKALVDYYGIDFDKPPLEEPQDEGNLIWNVGDTHEPWPLRDEADAVHIQRLETLLADEEAPHDDVFDTYKTLPSPGVVYLSIETIRALLHHLAIVERPSPIAMQRFLSILDDMKTAHIHIIRSEWTSAIHLAGHALGTVSADDLQSALYIWRDMEQRAGVKASFVTLNVLFNVAVKAGKFVLAETFIKELEARKLPLHRHFRVSLLYYYGVLQDGNGVRKTYQELVAAGDIVDTVVMNAVIASLIRAGEPSAAEHVFERMKRLHAQKAAPAPGHTFFKRTWRDKRLLSLHLAHEARRLNNMEDDEELKRLQDFAPIAPDSRTYGLLIRHQASTAGNIDRVNELLQEMRYNSVPLEGTIFIVIFHGFNSFGGVRYTSWTPDKLEKIWTQYLKALNGKLERTWLSSMAVIAALKAFSRCTDAERTVKAWEEIRKLWQPTEEELENVMRALRKLLPSQAQGSFDENPNRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y131</accession>
    <name>A0A9W8Y131_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein O-GlcNAc transferase</fullName>
            <ecNumber evidence="3">2.4.1.255</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_009023</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 41 family. O-GlcNAc transferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.255" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="421121at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097363">
        <property type="term" value="F:protein O-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000552">
        <property type="entry name" value="UDP-N-acetylglucosaminyltransferase (AFU_orthologue AFUA_1G03380)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11380:FF:000004">
        <property type="entry name" value="UDP-N-acetylglucosaminyltransferase (AFU_orthologue AFUA_1G03380)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000110">
        <property type="entry name" value="UDP-N-acetylglucosaminyltransferase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029489">
        <property type="entry name" value="OGT/SEC/SPY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44998">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44998:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE 110 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13844">
        <property type="entry name" value="Glyco_transf_41"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 8" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="563"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="923"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="domain" description="O-GlcNAc transferase C-terminal" evidence="10">
        <location>
            <begin position="1158"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="domain" description="O-GlcNAc transferase C-terminal" evidence="10">
        <location>
            <begin position="1418"/>
            <end position="1619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="283"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="378"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="641"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="60"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="378"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="404"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="423"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="644"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4364429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1649" mass="182921" checksum="FE7F89F58A987E11" modified="2023-11-08" version="1">MAMVRPYPPQPQSHLLSAPTPYGNLQPTPSHASRLRTAAFEGRSLAQHSTPQQYAHPLSRHNSYGSQSSESGRGSKPPEHMLRRKTPNGILAAAYDGTSVEKNGEPHATKHILLPVTAESKIPYGLKQELPLRSPGVNTGLGYGKQDGQSDWSPSLYFETGSGRTGQHLPQIDSMLNQIPPLQPLLPYQMYGQPYSGPMGPPMQAPLGPTVSNDQGPFGPYWHDGTYIPYRPAAMRDPRFLHHHSHNWNHPQHNPYFGYPYNSPAPNVSSYQQNLHINQSQNAFNNAGIPPVDFSPYPQPNSNFALDYQSPSLPPPLTQRPQDFLHSGQSTPVQEHGPVTPLDEFGPQSINGQSREKVFAWAHAVYVDLLRYLQSTRKSHSHHAHGRTSSRPHIYPKPPRQPGANFSGSSSSDTPSLERRVSFPTTNPYSSQSGLDATPRRPPMLHSRSSSNFSMGGADPRQPWHQMPPQHAMLPQMPGMEPSRTLRRMSGSVASVNQSLRHEVPPSMTAASALEAITKHCEESKWNWIDGILLGGCLAYALGDYQKAQDWYKHILTLDTDHVEAISNLAATLLALQQKRDAESYWRQAVRLRPSYFEAVEHLIGLLCSDHRGREAVQVIEEVERNLRFVKKADALKSLEAESTRSTSTVSESPSLSDSDRPVFEFEGEDESVFKDLDELPGSDQPGFGSSGYAIPGCDNGRILALVHAKGNMLYALGNNAGAAKAFENAVLIGAGPQARGIDGLIKRILSVVGYDGSERSPGGRRVAPSADPILLPPDSATKTAQLCFPPHGQLPGLKHVSSDGMARKAAISTTSNSLLSLAKIFQDGMANNSAKATAYQSNYGVREILALYYLSLSLQPSPSTANNVGILLASVQQSAPSKPLAVSSVVHHPQVPGLVPGSGVALALGYYYYGLQLDKRHAHLYTNLGSLLKDIGQLDVAIQMYEQAVACDQNFDIALANLANAVKDKGRIHDAIIYYKRAVKASPDFAEAVCGLANALNSVCGWNGRGGIAEERGARDRWHVDENGMLLDARIPGASSSGWIHKVVKLVEKQLADGEDWGRGTMDDHFIQDIIRPLTLTDGSAQDIKEKQNNVASALVKWKGQKWEGARLVRLVERAIRRMTWQWYQDRYVTFKQRPSASYKRPQLPAALAAPAAPTVLPFHTFTCPMSAKQIRLISQRNGLRISCSTLRVPWLPSTVYHPPPPPKPYLKVGYVSSDFNNHPLAHLMQSVFGLHNRKRVKAFCYATTASDGSEHRKQIEREAPVFYDTSSWSAERLVNQIINDGIHVLINLNGYTRGARNEVFAARAAPIQMAFMGFAGTLGAEWCDYLLADDTAVPPDTLRQWRRNIDMEDALVDDNSGGSDEKWVYGENIVFCRDTFFCCDHRQSAPDSQGRQPSWEEEQERRWAMRKEIFPDLPDTAIILGNFNQLYKIEPTTFRTWLRILQRLPNAILWLLRFPDLGETNLKQTAYMWAGAAVANRIKFTDVAQKHQHISRARVCDLFLDTPECNAHTTAADVLWSGTPLLTLPRYKYKMCSRMAASILKGALPKTPQGERAAKELIAGNDDEYEEMAVKLASDCIYHGHKASGRLSELRKMLYESRWSSPLFDTKRWVRDLEDAYEIAWKKWVKGEGGDIWLKQRQPSSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y199</accession>
    <name>A0A9W8Y199_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nuclear import receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MTR10</name>
        <name evidence="4" type="ORF">N0V83_010078</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">tRNA nucleus export receptor which facilitates tRNA translocation across the nuclear pore complex. Involved in pre-tRNA splicing, probably by affecting the interaction of pre-tRNA with splicing endonuclease.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y199"/>
    <dbReference type="OrthoDB" id="435593at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000266">
        <property type="entry name" value="mRNA transport regulator MTR10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051345">
        <property type="entry name" value="Importin_beta-like_NTR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057941">
        <property type="entry name" value="TPR_TNPO3_IPO13_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057942">
        <property type="entry name" value="TPR_TNPO3_IPO13_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058537">
        <property type="entry name" value="TPR_TNPO3_IPO13_4th"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363:SF53">
        <property type="entry name" value="MRNA TRANSPORT REGULATOR MTR10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363">
        <property type="entry name" value="TRANSPORTIN 3 AND IMPORTIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24138">
        <property type="entry name" value="TPR_TNPO3_IPO13_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24140">
        <property type="entry name" value="TPR_TNPO3_IPO13_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24139">
        <property type="entry name" value="TPR_TNPO3_IPO13_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="3">
        <location>
            <begin position="32"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4362961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="969" mass="109287" checksum="183CF7E8AAF77395" modified="2023-11-08" version="1">MAANGVQHVFEPVLAAHNTMQSGANRAQKEQAHQFLEQFQKSQEAWTTTLAILESTSADAAAKLFAATTLKGKIVYDLHQVPRPQLPELRASIIRNLATFHAGPKPIRLQLCVCLANLAIQMVEWKDVLKDIVGTLGTDPATLPCVLDFLRVLPEEVTHGRKIALTEHELTMRTAELIEDNAQQALDLLIRYATSSPAAAQNPQLLNCITSWMREIPLDSIINSPLLKVIIDDLSADEPFEAAVECLSALVSETRDVDEAMSSILSLYPQVTALKPMLSQAIQEEDTEKFKGIARIFAEAGESWVLLIARLPTDFRELVQAILETAALDKERDAISHTFKFWYDLKQYLTIEKYAEARMQSLGIYSQLVDIMIGHLQFPKPESGDEKDLFEGDREQEEKFREFRHQMGDVLKDCCEVMGVVECLQKPYDLIQQWVQTYGAQVGPNNVPEWQKLEAPLFAVRAMGRMVPPDENVMLPQLIPLIAAIPDHNKLRFQAVMALGRYTEWTAQHPDTLQPQLDFIMAAFEHSTKDVIRAAALSFKFFCNDCAGLLVNFIVPLQQFYAKHLNNLPISSQEEITEGVASVVARVPNDQIYATLKLYLDPVMQHLVTIANQAKDEPEQKLLADKINLITIFIEMVRPEIPSGQEHPAVKYCQEIFPMLGNIIAHFSNSIPILERVCRCWRYMVLSYRTAMRPLLPDLAINLTKGFDSSRQGCFLWATASIVREFSQGMDEVDPSLANDVYQFYEQQAKTFLRILSDLPPEELPDLIEDYFRLAADMALYFPSESIMSPLMDTILQAACSSLTLLKEDPIIATLHFLRDLLGYGRNSAPSSSFDNTRHEVPEALRNRVKQLVVGSGVQLVQRIMTGMMYSFPEGCFADSSGVLLDLFELMPEQVAQWVASTVAMLPQGSITPQESERFLNNIRQRIQTGDVRMIRTILQDFTTSYRRRNVAPREGLGRLEASRFRFSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1J7</accession>
    <name>A0A9W8Y1J7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3500 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009811</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1J7"/>
    <dbReference type="OrthoDB" id="4539697at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021889">
        <property type="entry name" value="DUF3500"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37489">
        <property type="entry name" value="DUF3500 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37489:SF1">
        <property type="entry name" value="DUF3500 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12006">
        <property type="entry name" value="DUF3500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4363516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="49784" checksum="41E629CDEDA5F510" modified="2023-11-08" version="1">MARRNSFEEASAGFRAHLPDLSSPRFTSAAKQNIYEYAKCMQDNRAPPWLYDLTKVWEKLLEEPYKGVTNDGTVRESLFGARDESADVAGAVNKVEELLNSLNEAQKKKISYPINAREWRAWSNPEFLLRPFGLRLEEVPESIAQSILRVIEASLSPEGYTKALSAMRINHFLGEVVQLPNIMNKYSYNFLLFGTPSTSSSSPWGWLLYGHHLDIACFFKGSQVVLTPSFTGAEPNIIDAGEWKGTEILHKEGNLGLKLMQSLSGEQQGKAQVFKNLRDEGMKQVYGNSNNDETKRDELITDTWGPDDQRHRCGAFRDNRVVPYEGVLVSSFTSEQQQLVLEICHEFLLYHPQSSRQIKLDQIQEHLNETYFCWIGGSSDADAFYFRIQSPVILVELDHHSGVFLTNKEPAKYHTHTIVRTPNAGDYGQAIRGANERLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1R4</accession>
    <name>A0A9W8Y1R4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_010321</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1R4"/>
    <dbReference type="OrthoDB" id="5283415at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF160">
        <property type="entry name" value="SAT4 FAMILY MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="215"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="26"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="295"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="300"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="342"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="382"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4363201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="410" mass="46193" checksum="93A4DF6F149DCB3A" modified="2023-11-08" version="1">MVESRQSHVLAIVGVFTVAASIFVALRTYSRYLGRNFHWDDYLILAALVLLIADTIGTWEYILLSGTGYHVWDVPKKSIHEQLVALRWNFTVQMFYHPLMGLIRASIIMFLFRMKDNRRRIRYSLHVVFWLNICYSVGTSLVNIFQCKPTRYTWTKPAMDYVDAAGNTVAAGTCIDSRTFVLTSCSLSIFMDLIIIPIPSVMVWNLQMSRRTKMLVVIVMSLGWIATGVSVGRFIVYYYRFAPTNTDRTWDIGIAISIAEPAVHIMTACAPATKCLFRIMFPSFGSTRRTPDYYEERHSTQLGRPSKSGSRTSRGLGNFSFGLSKNGGEEAGVEMGTGLGHSRGDVKSESVYDLKARSSVETIERHDVEDDDRRSLEVLEPTRTSTSKTAYTAGSEPIEAEPKHCLGHAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1T5</accession>
    <name>A0A9W8Y1T5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Integral membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1T5"/>
    <dbReference type="OrthoDB" id="3365211at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021369">
        <property type="entry name" value="DUF2985"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35872">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G07110)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35872:SF2">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G07110)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11204">
        <property type="entry name" value="DUF2985"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="395"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="427"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="584"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="483"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="629"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="639"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="682" mass="76859" checksum="183CF29CBCC2D85F" modified="2023-11-08" version="1">MDKLKAKTRSARNRLAEATSSGTGTGELHVPHQSLEPHDNSSTYAVNEGFLGAQPGTAARDFAAPDEQPVRQPTVEPPTSLQSSIDAGAHVPKPSRYGDPSSLVNHSTLSQNAQRARSNSAPNRASPQQHQRRPPVGVRQASIGIRRMPSNNALRQAPQQPNVSTSRLNAPLPALNEDCVLGEVPSRGSESTLTEPEPEQSRLRKVKSAVQTRIPFWSKKEQEKPQPHAAATRPEDLSANYTSDMVDVLDTIDPEVATLTSLTNMQNSLFIPNLPFFNRRPTYNLRRRRSEDESLEDINRYLDPVYAAQAEEQAQTAGPGLRRSESQTTMATVATIDSHLSESRYAVLPHGASLDGWSKDDKKEVNDHVRHMLHSRRSRFKRTMRGFGQYVRRPLGFLVTLYATLITLFGLAWVLFLIGWINVGGRQIYVVHIIDSVLVALFAVVGDGLAPFRAVDTYHMIYIAHYHHFTWSRRKKERLPELQDHNDLPSDNAPISAPRVVDPEDPENQWEFTVLTPKQQEKLEHHQTKFCKSHSFYKPHETETHYAFPLRLLIAIVVLLDCHSILQISLGACTWGIYYKRRPFAITTVILICSITCNATAGLLIWLGDKRTRKQDVLERMNRQELTEEAIEQVKKKKEKENESEREDDGEKKEKRRISLDVVRGKLATGKVGEKTETSAYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y292</accession>
    <name>A0A9W8Y292_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008859</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y292"/>
    <dbReference type="OrthoDB" id="3799444at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="457" mass="51992" checksum="4CB8F3CE6148C52B" modified="2023-11-08" version="1">MDPNMKIRRKDSYGRCGTEIQGPVAGIPEPPAEAAEREASYTSSVLSDADEDMMMVDEIELNDEDARRQEEEGYENMDEEDEEDEDEDETYIEHYGIEDHPYDTNFHRKLPRTRTRTVEAELDLTRSQSKQLRTCINEEMKKAGILGRMSSSPFSSSKAEARRQQHDATLRQIIQTAKHKLVFLRHTVVEEKRLDQWLLARMVIYSRKKKHDEFMTGTEEEVPSPSPSPPPSPTPPERPAPQSSSSVAYAGHDHDLRSVEADLAEGAFRGLRRGRRRERRRERRRGRERAHQSSSALAASFDNLHIEQPLDAALQNLNLMSHESPGTASADPPPPPASNAAMFDETAFLIRVVNSTTMPGIKVQIYELISSSSRRDSHPPLPEAIEDLSLENFTELLSAQVGFDVRGRISIVLPRGGLLMPAERRLRLTTEDQWRTVLWTVYIERERTVELVVERDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2A7</accession>
    <name>A0A9W8Y2A7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2A7"/>
    <dbReference type="OrthoDB" id="2596908at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="128" mass="13788" checksum="ABBA5B9D90447EDE" modified="2023-11-08" version="1">MTKEALQPLLIPEIDLSARNPVSNEDVTTTLSSQEASFVNGVFAKTASAPTNADPQAAASASAAVISAAPFVVPGTSLAFFPVGLVVTSFWTLMFFLAVGFGTFGRIQYRDQYRRRVRMEQARGVRTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2A9</accession>
    <name>A0A9W8Y2A9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008263</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2A9"/>
    <dbReference type="OrthoDB" id="3564818at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4365643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="160" mass="18511" checksum="CB1A2B6EEFF08DE4" modified="2023-11-08" version="1">MSVYAIPAAIDDKLRKPSDWPKWYNRLKYHAMSTGVWKQIDPDNNVQFHVYKAQPVGPELREITTEAPAPHTAAEIQLFGIHFAKWEVEMSNWEFLAERYENVQNWVYATVDPKILGRAKMAILISTTTSVTLQNLIKQLRYQMDPRGLTTNRVRGQVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2E6</accession>
    <name>A0A9W8Y2E6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_007775</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the paxM FAD-dependent monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2E6"/>
    <dbReference type="OrthoDB" id="1047367at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000201">
        <property type="entry name" value="Salicylate hydroxylase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050493">
        <property type="entry name" value="FAD-dep_Monooxygenase_BioMet"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13789:SF172">
        <property type="entry name" value="HYDROXYLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12410)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13789">
        <property type="entry name" value="MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="6">
        <location>
            <begin position="15"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4366140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="457" mass="50348" checksum="3A1FCA5A8724EB96" modified="2023-11-08" version="1">MTADQATVPHSWRRLDVAVIGGGIGGMSVAIALRRAGHAVTIYEKHDFAGEVGASVSCAANGTRWLHEWGVDVEKGDPVVLKKLINRDWRTGEPVSIFDLDDYEERWGYVYNMFHRQYMHAILKDAATQEEGAGTPAKLLVNYADIDVDSNIVSFQNGVRAKHDLIVGADGIGSVVRNIIGIRAEKRPAESSCLHANVTTEQAVKLGLVDYAQDNALEYWGGQDGTWDKIVLSPCNGGKLLSYYCFFPREKGDYISQSWGAEDRPTKELLAPYPELDSQVKAHLAIGVEIKPWRLWVHDPYPHIQKGNVCLLGDAGHPMMPHQSQGACMAIEDAAALGILFSPNHFTGDVQQTLQVYEKVRLPRATRVQSAAAKAAYNINERIGFSANKDSCSTYSVEDEAKKLTIEEMNAYDMYEDIKEKLALAQNKEYNNRFLAGLPMGLELPNGVIIGASVVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2L7</accession>
    <name>A0A9W8Y2L7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pkr1-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008429</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2L7"/>
    <dbReference type="OrthoDB" id="9626941at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013945">
        <property type="entry name" value="Pkr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28251">
        <property type="entry name" value="V-TYPE ATPASE ASSEMBLY FACTOR PKR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28251:SF1">
        <property type="entry name" value="V-TYPE ATPASE ASSEMBLY FACTOR PKR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08636">
        <property type="entry name" value="Pkr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="23142" checksum="B9827983CAC249FF" modified="2023-11-08" version="1">MASFFENLWLSIFTPGPTPTLLVATNASFAALQLLLFAMLLATYSIHFIILSVLCGGLWWAINWFATEIRAAQAKEDEAKRIREARRGKEQGALGEDDGETTDTGDDKEVDTEVEQKYKQPPKPRSNPPRAPRPETQSTVFVERPSESRREATPQASSAGGGGPTAASTTGATTKLAPVVDDALKRRKGLGESTGDLSTDSEWEKLSEGSEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2U0</accession>
    <name>A0A9W8Y2U0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008851</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2U0"/>
    <dbReference type="OrthoDB" id="3542212at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42052">
        <property type="entry name" value="ABM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42052:SF1">
        <property type="entry name" value="ABM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4365233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="22467" checksum="6535BAB0368E61FE" modified="2023-11-08" version="1">MPVLEVTQLRVKGLSADDPALLQSLSVVRGKLKTNSRFYHCIEDPTLIYILGIWPSLDAHLEFLASPERDEILGPQEDMLDFCWSVHMGLDGMSSLPLDAPVLAIEIMKIKGDCVEAFDQAVIRHTQHILGSHPFKVAHGWRCDAAAGSHEALIFTGWENTQAHVTFAVNQEGHRDGDKAALNGQYEVIQVHHAKNLERKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y300</accession>
    <name>A0A9W8Y300_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Endothelin-converting enzyme 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_007299</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y300"/>
    <dbReference type="OrthoDB" id="6475849at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08662">
        <property type="entry name" value="M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1380.10">
        <property type="entry name" value="Neutral endopeptidase , domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000718">
        <property type="entry name" value="Peptidase_M13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018497">
        <property type="entry name" value="Peptidase_M13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042089">
        <property type="entry name" value="Peptidase_M13_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008753">
        <property type="entry name" value="Peptidase_M13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733:SF167">
        <property type="entry name" value="FI17812P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733">
        <property type="entry name" value="ZINC METALLOPROTEASE FAMILY M13 NEPRILYSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01431">
        <property type="entry name" value="Peptidase_M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05649">
        <property type="entry name" value="Peptidase_M13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00786">
        <property type="entry name" value="NEPRILYSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51885">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M13 N-terminal" evidence="9">
        <location>
            <begin position="89"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 C-terminal" evidence="8">
        <location>
            <begin position="547"/>
            <end position="745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4366774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="751" mass="83545" checksum="FAE2576675132A26" modified="2023-11-08" version="1">MAEKQPLIAPATHARVEACQHVQRRSKMGTAGKLLVIAAAAGLVYYGAPQGDDVVVDKKAEQCLTPACVHAASEILYNLSPDYKELDACDNFEELVCGGWRDRHDLRADQGDAFTGTIMSENSQMLLRHILEAPYPKDSDHSYFSPMQLQTTAKSADEENFDKMKAAYDACLNEDKIKGIGAEPLLHVLDEIKKSFPTATSKSHATKDAILLLARYGVTTLVAAGTGADDTDPDTVVVSVSAPWSFGLPSKERYEDEKLVEKYRGVAVEVLGALYPDQNKAAFAGIVDLEKKLAAASPSEEEREDVTKYYNPMLIEEAAAIAPEIELEALLADQAPSGSKIERVIVMTPNYLKQLSVILAATDREVLQSYFIWKAVQSFSSYVDADAVKPYRRFVNVLAGKDPDSAPERWRTCVGHVDNGLGWILSRFFVEKAFSAEAKEFGDTIVTDIKTEFTKKLNTADWMDKDTTKKAVEKVHNIVQKIGYPTKSPDIMDPSKLSHYYDSVNVSSDAFFSNALAMRSFAVDDEWSSLGKPVDRDQWDMTVPTVNAYYNPPGNEIVFPAGIMQFPVFDVEVPAYMSYGAFGSVAGHELSHAFDSTGRHYDQNGNYTDWWSNATIDAFKEKTDCFVKQYANFTVPGSNDEPLHVNGRLTLGENIADAGGLSASFQAWKRRAGEKPNKDLPGLEHFSQEQMFFVSYSNWWCGMTRKDTAINRIYTDPHAPKWARILGTMANSREFRESFQCKVKQPTCQLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y351</accession>
    <name>A0A9W8Y351_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008485</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y351"/>
    <dbReference type="OrthoDB" id="5371740at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44229">
        <property type="entry name" value="15-HYDROXYPROSTAGLANDIN DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44229:SF4">
        <property type="entry name" value="15-HYDROXYPROSTAGLANDIN DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="32149" checksum="B8BCA3DA9AB9B0DE" modified="2023-11-08" version="1">MASKKVALITGGASGMGLAVATALSQRPDWDIHILDLDPSTGIPIASSLNATFHECNVTAEATLSRIFRTVFSQSNRLDFVFANAGIPERANFYDEQQDDSGDGPPLPVKGMHAIVDIDLKSVITTTYLALHYMRKSPRYTEKNIVMTASCGGLYPSYYSPIYTATKHAVVGFMRSIAPFFYHKAGIRVNAVCPGVVKTNLLSSQEWANFPQEYFTSVEKVASVVCMLVDGKDDGSGKVGKEVEGGLASIGGVLNGRAVEVSGKNHYYREMVPFADEAMEEVMGSTNIEVLENVQSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y418</accession>
    <name>A0A9W8Y418_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008138</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DapA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y418"/>
    <dbReference type="OrthoDB" id="191315at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00408">
        <property type="entry name" value="DHDPS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="DIHYDRODIPICOLINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128:SF47">
        <property type="entry name" value="DIHYDRODIPICOLINATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="2">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR001365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="314" mass="33219" checksum="3BA4BA92A9D3BFCD" modified="2023-11-08" version="1">MAPNPPAAGVWCPAVTFYDTTTSHLDLAAQKQYFTYLSKSGLTGLVILGSNAEAFLLTRDEKIALVKAAREAVGPDYPLMVGVSGFSVPQVLENIRDAVAAGANYGLLLPAAYYGAATSKEVVIGFYDEVAKTSELPIVIYNFPGICNGVDLDSVTIAELAKRNPGKIVGVKLTCGSVAKITRLCAELPSETFSVYAGQADWMVAGLAVGSAGCVSAFSNVFPKVCSKVYELYTSGNTKEALELHRKAALAESPIKAGIAPTKHAVSQYSAKAAGIEDAEEKLKPRRPYLPVGEAMRTSVRSAMSELAEIENNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4G2</accession>
    <name>A0A9W8Y4G2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phosphoribosylamine--glycine ligase</fullName>
            <ecNumber evidence="2">6.3.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Glycinamide ribonucleotide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Phosphoribosylglycinamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ade1</name>
        <name evidence="16" type="ORF">N0V83_007518</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the GARS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.13" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4G2"/>
    <dbReference type="OrthoDB" id="2018833at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000006">
        <property type="entry name" value="Phosphoribosylamine--glycine ligase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000006">
        <property type="entry name" value="phosphoribosylamine--glycine ligase, chloroplastic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000018">
        <property type="entry name" value="Trifunctional purine biosynthetic protein adenosine-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.600.10:FF:000001">
        <property type="entry name" value="Trifunctional purine biosynthetic protein adenosine-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="entry name" value="Phosphoribosylglycinamide synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00138">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020559">
        <property type="entry name" value="PRibGlycinamide_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472">
        <property type="entry name" value="PHOSPHORIBOSYLAMINE--GLYCINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLAMINE--GLYCINE LIGASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01209">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00184">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="15">
        <location>
            <begin position="118"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4366988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="433" mass="46185" checksum="40CE6F83867EC9DE" modified="2023-11-08" version="1">MTDKLRVLLVGNGGREHALAWKLVQSPAIEHIFVVPGNGGTATLDKKVTNVSDVKDTDFAGLVKFAKEKAVNFLIPGPEAPLVAGIVDYFAKNGCEGIRAFGPSEAAATMEGSKTFSKDFMKRHNIPTAAYENFSSYDAAKSYLDTISHNIVIKASGLAAGKGVIIPQSKEEAHAALRDIMLDKEFGAAGDEVVVEEFLEGDELSILSFSDGTTIKSLPPAQDHKRIFDNDEGPNTGGMGTYAPTRIAPRDVLDHIDREILQPTIDGMRAEGMPFKGVLFTGLMMTKNGPKVLEYNVRFGDPETQSLLALMNSDLAEVMVACAEGRLKDVNVSVSERSAATVVVAAGGYPGSYAKGTPMTLDAVPEDVVLFHAGTSYSSSDKTLKTSGGRVIASTATAPTLEEAVAKAYKGVECIHFEGMQYRKDIAGRALSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4M6</accession>
    <name>A0A9W8Y4M6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_008455</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4M6"/>
    <dbReference type="OrthoDB" id="5354458at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056687">
        <property type="entry name" value="DUF7785"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057199">
        <property type="entry name" value="DUF7877"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25009">
        <property type="entry name" value="DUF7785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25289">
        <property type="entry name" value="DUF7877"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7877" evidence="4">
        <location>
            <begin position="53"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7785" evidence="3">
        <location>
            <begin position="490"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="298"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="352"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="613"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="769"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="828"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="486"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="303"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="632"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="654"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="693"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="736"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="783"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="842"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="858"/>
            <end position="887"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4365834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="887" mass="94918" checksum="5546F876294C6415" modified="2023-11-08" version="1">MATNGTLTPPSLPPATTSPSIAKRKHIGPHTITLNGASVSAQGKAANGTTYTSQMVLKDILAVLKSYDTQPSILAHPITSAITRSPSGEADSKRAKLTPPAGSSTITSLIQDGSYDSLQALEKDVENATTEILAFSGGSESSALAASPAETTLQAQVLAFRKIAKSLIDREEARKAQFEVEDVDVDAEGEDEIPKEEGTPVPIKEEEPNIPESRTVLTLYGSAPPKQLFSSLQQPHRVQPADGTSTLDASVKVTLPLRETNLPNMISTTEVYPLSDPADSKKKKTSTIGEIFRAPAHLPQISPPKSTRPTTAKGNNTITFAPLEAPKPRRKGSQSYAHQNLSSGFWLGYGGVDMPKDQTSPTAKQKSRQRALSMGEAQQPPSGAILVAVQQAKEDALFRSAYSSFAPTRDDSSAVIPEETKNMVWWQKVGEKRFNETFPVDPALLGAEEVTDTDVNGVTHEDEGFEEAVANFVPVEEDALPGAEEKSEEEKDTDELLKEISELLETLASYQRIRNSSLTTNPLTPVVQNSSLATLAGSPSTPSTEEVDVYQILKSQLTLLISQLPPYAVAKLNGDQMDELNISRTILFDTKEYKGVLEEDQLSRLAKAPPVAVPTPATLTRAGSGAHAHYPGSSTQYSRPNPSVPQSGARPVYPQQQSLHRSSSMHLQRSPSGQTQSFQAGGASYAPPARPSYSATPSYNQQTPRPAYGSTASGLYQSQRSAQPSAFGGSQYFNSTPQTQPQNRYTQQQTQNGYFSRSQNVAPMYNTAQTPQARTGSPMKPGQTPSLSSMTSRPGYGTPVGGGQMRSTYYGPSQYGTAPAPSTPTTYNGMASNPQQMMIDRQQAQVAAQSQARLAAQNSFSSRQGSGTPQPPNGSYSGQVNGASMST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4X0</accession>
    <name>A0A9W8Y4X0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S54 rhomboid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_008336</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4X0"/>
    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050925">
        <property type="entry name" value="Rhomboid_protease_S54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731:SF14">
        <property type="entry name" value="PRESENILIN-ASSOCIATED RHOMBOID-LIKE PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731">
        <property type="entry name" value="RHOMBOID PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="8">
        <location>
            <begin position="114"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4365716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="272" mass="30216" checksum="3F7AAFC007225F5D" modified="2023-11-08" version="1">MSALLRFSRLVRPTGTLQPNKFAPKTMTSQFFTPYRASARGGLSRSRFYSSNYRPGSPYQLSTNMGVLYSLMGANIAIYFYAVYLKAQFMQGYQLPFMKFMQNMTLNFTEFRNGRWWTAVTSTFTHVDFGHVFSNMFTMYFLGSFLATAPQVTPGRFLFIALGSGISGSAGYLCQQYLKVQSNPGVPDYSRGLGFSGAVMGICSVAACLAPTTKFQLYGIVPVPLWALVAGYAAYDGYYLNSADTRVAHSGHLGGLAFGIMYYVLKLRGMRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y599</accession>
    <name>A0A9W8Y599_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dienelactone hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y599"/>
    <dbReference type="OrthoDB" id="17560at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17630">
        <property type="entry name" value="DIENELACTONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17630:SF87">
        <property type="entry name" value="DIENELACTONE HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dienelactone hydrolase" evidence="1">
        <location>
            <begin position="30"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="285" mass="30925" checksum="8867C2C2406B4898" modified="2023-11-08" version="1">MASSCSSECIKGTIHSGQPKGAEELIHGLNTYVIGNRTNPRGIIVVYSDIFGLGLPNNKLIADAYAATGEYLVYLPDFFKGDAVGLKVADALLPVDATKQSTFAKYSGILANMPTFLMWMTRHKQGPTNQICMDFLGELRRATPASRKIGIVGMCWGGKYAVRAGLESNMVEADGGGVKKPLVDAVVALHPSHLAIPDDVEGLVVPVSYGWGVEDIAVSFKQKGEVEAVHDRAQKAGKKVPDMEHKVYKPGRHGFAVRGNPDDPLERKCLEDSEAQVLAWFNKWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y646</accession>
    <name>A0A9W8Y646_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peroxisomal ATPase PEX1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Peroxin-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RIX7</name>
        <name evidence="10" type="ORF">N0V83_006830</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y646"/>
    <dbReference type="OrthoDB" id="27435at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000081">
        <property type="entry name" value="AAA family ATPase/60S ribosome export protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000149">
        <property type="entry name" value="Nuclear valosin-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000365">
        <property type="entry name" value="Ribosome biogenesis ATPase RIX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="198"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="508"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="69"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4368473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="736" mass="81982" checksum="8DFAD3DDF0771B40" modified="2023-11-08" version="1">MSSRGRSSLKQGLDREIYQVVLKYLDDKNESPLKLRPTAVYDYISRSNSSLKRRSKKQLEDSIERVIDALRDDASGDDEDEMAELEGEFGSDEKSKEGKSTDWMNRQIVNQWATPTANGEKSEKNKKRDGEKAKGERESKRQKKSSAPAPDAKINIAPPTGVCLKDLGGVGKVKRQLKEHLVLPLLSPQEYVDRKIPIPRGILLHGPPGCGKTVISRAFAAELGVPFIEILGPSVVSGMSGESEKQVREHFERAKEVAPCLIFIDEIDVIAPKRDNAQSQMEKRIVAQLLISMDSLSMDTNDGKPVIVLAATNRPDSLDPALRRGGRFDTEINMGVPNESMRELILKALTREPKLTDDVNFTALAKRTAGFVGADLKDLVSKAGTWSMDQYREALEKQAAEGEMDIDDQANRDEDFAVDRSIARLIKRVRDTEASRPEGFEDTAISMEAFEAVLPTITPSSKREGFATVPDTTWRDVGALANVREELEMAICEPIKDPQKFANYGISAPTGVLLWGPPGCGKTLLAKAVAAESKANFISVKGPELLNKYVGESERALRQIFLRARSSVPCVIFFDEFDALVPKRSTDLHEASARVVNTLLTELDGLSEREGIYLIAATNRPEMIDEAMMRPGRLETLLYVELPKPEERVDILKALIRQRGVIDVELAEIGRRDECKDFSGADLESLLRKAGQHALRRRAAKIEEVDFVEAARNIKPSVGDIRKYEKLRERFEAKLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6D8</accession>
    <name>A0A9W8Y6D8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Autophagy protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ATG18</name>
        <name evidence="6" type="ORF">N0V83_006659</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6D8"/>
    <dbReference type="OrthoDB" id="1667587at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="267"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="267"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="282"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4368303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="433" mass="47307" checksum="0BABCE2D3570305C" modified="2023-11-08" version="1">MTAMNFVTFNQDHSHLGVGTTNGYRIYTTDPFNKQSESREGDVSSLEMLFSTSLVALTLSPRVLRIQNTKRHSTICEMTFRTAILAMRLNRKRLVVVLESELYIYDISNMQMLKTEKTSPNPNAICALSSSSENNYLVYPLPTKAAPATFQPPSHAPPKTDHIAPTSGEVLIYDATKMEAVNVIEAHNSPLSCIALNNDGTLLATASEKGTIIRVFSIPDAQKLYQFRRGSIPARIYSMSFNTTSTLLSVSSATETVHIFRLGAPNTSRSNSVSSGPNKPLSESRERSSSRASEENIDEFGASTADIASTEQRKPLNPTFASMIRRTSQTVGKSFAATVGGYLPSAVAEIWEPARDFAWVKIPKSPNSSSSGPMRSVVALNNNGPQIMVVTSEGNYYVFNVDLEKGGEGTLYKQYSLLEPSELTSSASQDLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6N4</accession>
    <name>A0A9W8Y6N4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006708</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6N4"/>
    <dbReference type="OrthoDB" id="3763466at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4368352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="327" mass="37742" checksum="B92C7B3D637E1253" modified="2023-11-08" version="1">MSDPWLEPFSNLGNPLLKRRITSYARTALDRLPRELRDEVYKHLWESYNFTPSSAFRRKDMLGKGEDSIHNTLSYLYIQFLSPSYVGRQFVQEALQWFLQHGCAGRYVTAAYLGIFLTNHYPGINITPANSNMRSLNIGIDVGDFDRVDFLLRQRLYSIKAQFAPLLALDLIKGFKLLIRVSIPVWKNHCPDARRLPTICAQLKNVMQSFEKKGSKVVIEFEFAPAAAKWHPEPLRNGFDYHPFTVNTAPRSFYSTSRVRYFNPPWNNARLVWTPNLSRVPPIRGKAAVDLLDATGIGWADYMRNELNVCRCVILEESVFRFRSNNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6X7</accession>
    <name>A0A9W8Y6X7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">proline--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Prolyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_007075</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Pro) + L-proline + ATP = L-prolyl-tRNA(Pro) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6X7"/>
    <dbReference type="OrthoDB" id="10267474at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050062">
        <property type="entry name" value="Pro-tRNA_synthetase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42753">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME PROTEIN L39/PROLYL-TRNA LIGASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42753:SF2">
        <property type="entry name" value="PROLINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="1"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4367492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="518" mass="57742" checksum="44F26F1884419C6C" modified="2023-11-08" version="1">MESKAHSGVFHLLPLGLRVQNKLETLIDKYMQSIGASKLSLSAITTEALWRESGRYSKNSELLRINDRRESGFLLSPTHEEEITALVASLVHSYKDLPVRLYQVGRKYRDERRPRQGLLRAKEFMMKDLYTFDHSPQNALKTYEAVRQAYNNIFDELKLPYLVADADSGNMGGKLSHEYHFVSPKGEDNVWSCDSCEYVANEELVEKKTASPKKVQSPQHTARYTFRGISVDRKTEIQVHLPNPSSAFEKDEHLPWSRISEFANLHAIKNALPDDIELDTGMEASTLAWCLSDSDKVHRPIIHLFDRALHPSHSSPDLTITRPGDACPRCSTGTLSVQKAIEVGHTFHLGTRYSTPLNALVAVPENPTKEPMQMGCHGIGVSRIIGAVASLLADEKGLNWPRVIAPYEAVILTSPQTGEEEAVQVYDALCSGTRSDGGQGAVDGKGEVVDLVLDDRPIKSLGWKLRDADLIGYPVVVVLGRAWKERREVEVQCRRLGVKQDVGLGELKSVVLGMLEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7C3</accession>
    <name>A0A9W8Y7C3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_006132</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7C3"/>
    <dbReference type="OrthoDB" id="3797060at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="209"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4369049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="490" mass="56257" checksum="826B8E76B474DCEA" modified="2023-11-08" version="1">MAKKATPKPKKVDVKKPDPKKSNPKQPDPQGSELFFRGGASVLKPNHITRYIYPSLCGYPKACLRLQPPANSKKRSRPQGANSWGEDGHTFLTISKALNPAPTNAQFTQWSTTEVSKDLKSKNPLGRAMVRVTRYAGNTVLESGKFALKKGAPLVYVVKRTTVIPLGGSDVGKFGREEEEYPRPDIEAHVTLYRKPPPSKQAEFLGDSCLVCGRDWFTESEYACQLKCKHFVCLECLTWHIDSSSGPTIGRGTIHPHETDPNTKFFRCPLCKDINPLTTDRATIIRADELPWWRWKITMRRMEREMKDNWLTRLQTLPHDDPPWFREIPESWDQDRQANDIRVYVRYEHAVKFMEVSKKIWGELPYGFSLDNPVGSREAVALEECLNGELKRVSMERRRTFNTKELVEHMVRVGNEAVKPVVVEDESARLGNPIFPPGYQAYRQFLSEWTARGCFLSPLGRMDVLGFMKRMDEHKGKAWWKDERDVFFDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7F7</accession>
    <name>A0A9W8Y7F7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein transport protein SEC31</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Protein transport protein sec31</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SEC31</name>
        <name evidence="17" type="ORF">N0V83_006177</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPII-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat SEC31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7F7"/>
    <dbReference type="OrthoDB" id="542917at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.940.10:FF:000007">
        <property type="entry name" value="Protein transport protein (SEC31), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000193">
        <property type="entry name" value="Protein transport protein SEC31, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.940.10">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040251">
        <property type="entry name" value="SEC31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923">
        <property type="entry name" value="SEC31-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923:SF11">
        <property type="entry name" value="SECRETORY 31, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="7 15" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="254"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="475"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="775"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="812"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="842"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="874"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="926"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="960"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="983"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="1002"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1030"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="1045"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="1084"/>
            <end position="1108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4369094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1243" mass="133467" checksum="A053BD9869516909" modified="2023-11-08" version="1">MVRLREIPRTATFAWSPGSTQPLIATGTKAGAVDADFSNETQLELWELKLDESEQGVELQPVATVNVESRFNDIAWSQPTEQYPRGVIAGALDSGALVLWDADKLRAGASDAQIEQIDKHTGPIQAIQFNPFRPHILASAGAKGELYIHDLDDESKSFRLGKAGANPDEYTTLDWNKKVAHILATGSSGGFVTVWDVKGKKENLTLNHYGRKTVSAVSWDPDVPTRLVTAIPTDQNPLVLVWDLRNTNAPEKTLQAHDQGVLSLSWCAQDSNILLSCGKDNRTIAWNPHSGEQLGEFPVVTNWTFQTRFNPSNPNLLATASFDGKIAVQTLQNTGATVDQSKSTAQAPEGEDFFAQTHAEPQGASFTLKTPPKWLKRRAGVAFGFGGKLVRFGIVDSKSKVTISTFAVDSGVSTASEEFDKALEKGDLTSICESKIAKAASDEEKADWTVIETLTSDNPRSKLVEYLGFADTKEEEPVEVKKETQTNGDADEGASFFDNATDEGNFLSDLAASKGAKTNNPFQVYTGSESEADTKITRALMLGNFNVALDVCLKENRLSDAFMIAICGGEKCIAKAQAAYFKRQSDAPNYLRLLASVVGKNLWDVVYNADLKDWKEVMATICTFADQSEFPDLCEALGDRLEEAISGEAGSFRKDASFCYLTGSKLEKVVVNWAQELQENEAAGVEQSSGDNSFSIHARSLQDFIEKVTVFRKVTAFKDPEQQKDADWKLEPLYAKYVEYADIASSHGQLAIAEKYLDLLPPKYPAADVARNRVKQATKKGAPQGAAAHRSAQPAVAQRGQRVVPSYGGLPAQSTPVQQTASPYAPVNPLQGQLQQGASPYAPTPANPLAPPTQARNAYTPAGYQAPSQPSIPQPGYGYQAQQQHPIAPPPRNFASSPSIVPAANRPNIPAWNDTPDFGPPKPSSRRGTPLNTVASPFPNQQQPLAGPPQGPFSPGARSTPPPPPKGPPQGPPRMTSPPSGPPQAGAPNPYAPSPAANAYAPPQPSGFAPPPQAPVQRGASPYQPPSSSAPPSNRYAPAPGSQPSAPPHGGVPPPRNIAAPPSQFSPVPSPYAPSPAQQAPPQAAAPPPRGPPQGPPRAGPPQGGPPRPDSRPGTGQSQTAPPANAAKYPPGDRSHIPNASRPIYEILEADMQRIKAKAPAQYKQHVADTEKRLNILFDHLNNEDLLKPDTIQQMNELASNIQAKQYDAAVAIFSDLMTNKNDEGSNWMVGVKRLIQFSKSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7N4</accession>
    <name>A0A9W8Y7N4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Enoyl-CoA hydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_005319</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Mycotoxin biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Siderophore biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7N4"/>
    <dbReference type="OrthoDB" id="2139957at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000074">
        <property type="entry name" value="Enoyl-CoA hydratase (AFU_orthologue AFUA_2G10650)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF158">
        <property type="entry name" value="ENOYL-COA HYDRATASE (AFU_ORTHOLOGUE AFUA_2G10650)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0843">Virulence</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4369557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="30098" checksum="C093335C44B3AE49" modified="2023-11-08" version="1">MADLETQPPPTKFLQLSYPAPRVVLATMSRPKDLNCMNTSAQWEMDAFWKWLDLEPSLTVAILTGSGRAFSAGADLKEWDNSMAADADPKDRLGNAPAFKPLSRRLGKKPVIAAVNGLSMGGGTEFAVNCDLVIAAENAYFALPEVKRGVSPIGGALPRIIRTLGLQRASEFALTGRNVTAKEMHSWGLVNSVVPLDKLIEEATRYATMIAENSPDAIICTRAGLREGWESASVERAVEITLEREFAQLQRGENILEGLKAFTEKRKPIWKASRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7N5</accession>
    <name>A0A9W8Y7N5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Proline dehydrogenase</fullName>
            <ecNumber evidence="2 5">1.5.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PUT1_1</name>
        <name evidence="7" type="ORF">N0V83_006299</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Converts proline to delta-1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23784"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.5.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the proline oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.2" evidence="2 5"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7N5"/>
    <dbReference type="OrthoDB" id="5464at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004657">
        <property type="term" value="F:proline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002872">
        <property type="entry name" value="Proline_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015659">
        <property type="entry name" value="Proline_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914:SF0">
        <property type="entry name" value="PROLINE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914">
        <property type="entry name" value="PROLINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01619">
        <property type="entry name" value="Pro_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proline dehydrogenase" evidence="6">
        <location>
            <begin position="133"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4369214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="472" mass="52013" checksum="A2D37A9F0B5A684A" modified="2023-11-08" version="1">MLQPRFGGNALRCVANKKGLTGLNTFEVRWASTSAVPRGKTETIQIPSQSLKQHPLAAMPTGMLLRSLLIATVSSNKFLLIPSLHLLSFFSKPDRSYLFNIDRNPVLKALLKRTLYNQFCAGETEKETRACVKQLKDLGFKGVILTYAKEMVFDHKSKSPNHHTSDKFVEEGAKAVANDTDIEAWRVGTLKTVDLISEGDILALKTTGGGPAVAESFSKGGLPPQQMLDALDEIAVKCKERGIQIIVDAESQHWQKGIGRTTLELMRKFNTDGKAVIYNTYQAYLKITPSVLAQHLAEAEKDGFTLGLKLVRGAYILSDDRTLICDTKEDTDNQYNGIAQGALRQQIGDFGASGPNARPFPSVNLFLASHNRDSVLSANKLHRQRLEAGLPTVPVAFGQLHGMSDEVSFSLLAEKGENEMPPEVFKCSTWGSMGECVGYLLRRAVENRDAVLRTKDEFAALRAETRRRVFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8T7</accession>
    <name>A0A9W8Y8T7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein gts1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">GTS1</name>
        <name evidence="3" type="ORF">N0V83_004930</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8T7"/>
    <dbReference type="OrthoDB" id="10266696at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000081">
        <property type="entry name" value="GTPase activating protein for Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051718">
        <property type="entry name" value="ARF_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705:SF7">
        <property type="entry name" value="ACTIVATING PROTEIN FOR ARF, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G09120)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arf-GAP" evidence="2">
        <location>
            <begin position="14"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="531"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="301"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="437"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="562"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="614" mass="67258" checksum="1015601F87C2EFB1" modified="2023-11-08" version="1">MASALNKRQQARNERALQELIKTVPGNSTCADCGARNPGWASWSNMKRIGNSESNKTYNPRNVKPQIPIDIDEVDSAMERYIRQKYEQRIYMNDSRPGTRQNTGSTSSEDRPPPLPPKPTGRFGFGLRKAATSPRNLTPPVSPGLGSFAQDASPPRVNKASRVFGSNISTSGDGMDSKLSTLRDMGFPDDKRNSTVLKGLNGNLDRTVEALIRLGEQNPGKSRGSTPTPPTKPGTNGLSFDRTDTAPAVTNSTNPFDALDAAPPPVEQPQVAPIQTSHLPYGGSSVQPTSPSNSYNPFFSQAPQAPYQQQGYPQQQQQQQPNYNYQSNPFGQQQQSLEQSFQGMQITSQQPQQPLFPNRTGGYAQSQPSPYGQTNPFHQTSFTPPPMPQIPEQYSSFYPPQPQYQSMSSPISPGNPFLKSSRSQIFTPTNSNPFGQPPTQQQASQQQQAPQPQQPQNPWMQQQQRHVSSPQPSNPWQSQQQSYQGQQTYQTQQPNYQNPQQSFLNQQQGFQGNPNPFDKSSILSLYNQPQLAPQRTEQPQVPSAPAPASAPPAQVSGHMNPFASNSGAQQGQPLSAAQTPGVRHVSNESVDFAGLMGGRHSPDAFSGLSASFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9U8</accession>
    <name>A0A9W8Y9U8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_005501</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9U8"/>
    <dbReference type="OrthoDB" id="2117453at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37451">
        <property type="entry name" value="MARVEL DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37451:SF1">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="6">
        <location>
            <begin position="9"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4369738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="164" mass="17961" checksum="CC1470B73CC9B367" modified="2023-11-08" version="1">MAISWIHPVRGVQALLSVVVLGLMAYVSSWWSSHWRQLSPSEVNFMIFAPAWSILALTALLVIPMKFSHLLASPAARYGMLGLEALTMLFWFGGFIAMAVFLSDRICFGTVCDVARAGTVISAFNWVAWTVTFVFGVIGVVKGGAMSFGKKRTVTKEVDMHQGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9V8</accession>
    <name>A0A9W8Y9V8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9V8"/>
    <dbReference type="OrthoDB" id="3830006at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="413" mass="46686" checksum="87DCD15BF4706339" modified="2023-11-08" version="1">MSTEIPSTIDRLSPSWIRRSRSGTKKSAKEADSRPTTSNGLLSPESASASSASEDSRSARSARSDRRPSIREMVNRLRSPSSASLASNKSQDVDFSEVENWFYGFRKYNQLVTTKISPDRAYPSSEFSKATKALTKNLGGQFIHGLPEATFDLSLLWCPAGTLTRKTDGNEPSWSWTAYDGPVNFPFDHTSCPDYYKIPRTETELFRSEIVNFHIGPAKDPYTIRREKGEKLRMKLPSWFHAPRGSDSSIESNTLRFTAATISADRFRADPLVCEEEGVMVKQLHCSELSNDQNQHCGVFMEGPSILEPSEDGPFEFILLSRNLRAKHDEDTRKPRVPTMHPPGTPVWDGERFVWDLEVTDFDETLYAPGPWKMLNVMLIKWVGDHAVRLAVARIHEDAWNAESPVRKEIVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA13</accession>
    <name>A0A9W8YA13_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004591</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA13"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="147" mass="16806" checksum="9CC50BB434FBE438" modified="2023-11-08" version="1">MATEDHYSRDREVNHARRLRDEEEEADRRIKAAEAAAAAAAAAQAEDDDDDDDDDDDDDDDDDDDAYRREQEENRLRRLRAEEETARRADEDAFRDLRAAATAPSMDRLSPPPNSLLLHSRSDHEDANLLVLKDNRGEDALTRVTTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAN5</accession>
    <name>A0A9W8YAN5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Transcription elongation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">tfs1</name>
        <name evidence="19" type="ORF">N0V83_004091</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the TFS-II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAN5"/>
    <dbReference type="OrthoDB" id="44867at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001139">
        <property type="term" value="F:RNA polymerase II complex recruiting activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031440">
        <property type="term" value="P:regulation of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00183">
        <property type="entry name" value="TFIIS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13749">
        <property type="entry name" value="Zn-ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000001">
        <property type="entry name" value="Probable Transcription elongation factor S-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.30:FF:000003">
        <property type="entry name" value="Transcription elongation factor S-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.10:FF:000007">
        <property type="entry name" value="Transcription elongation factor S-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.30">
        <property type="entry name" value="Transcription elongation factor S-II, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035100">
        <property type="entry name" value="TF_IIS-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003617">
        <property type="entry name" value="TFIIS/CRSP70_N_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036575">
        <property type="entry name" value="TFIIS_cen_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006289">
        <property type="entry name" value="TFSII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01385">
        <property type="entry name" value="TFSII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477:SF0">
        <property type="entry name" value="IP08861P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477">
        <property type="entry name" value="TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07500">
        <property type="entry name" value="TFIIS_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006704">
        <property type="entry name" value="TF_IIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00510">
        <property type="entry name" value="TFS2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00509">
        <property type="entry name" value="TFS2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46942">
        <property type="entry name" value="Elongation factor TFIIS domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="19" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="17">
        <location>
            <begin position="6"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS central" evidence="18">
        <location>
            <begin position="144"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS-type" evidence="16">
        <location>
            <begin position="261"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="79"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ4372317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="303" mass="33734" checksum="5D00B05CAA34D095" modified="2023-11-08" version="1">MAMNAKEIEFRAKAIGKAVQENAPSESVLKFLNELKTGVHANEDLLRQTKIGVTINRLRTHKDPAVQRLATELVSKWRDEVKKQSKKGTPAKVAAANGSASPAPPPSGTASPAPSQAKKKHDVPPEKRNHKTDNVKYQVTGSEARDACVRLMYDGLAFMSEALPDEILNVARQVEAAAYTNATSVNEAYKGKMRSLFQNLKNKSNPQLRKRVLSGEVPAKQFVVMTHDEMKSDARRAEDEKLEKENMKEAMVAQVEKSVSKEFQCGKCKQRMVSYSQAQTRSADEPMTTFCECMNCGNRWKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC40</accession>
    <name>A0A9W8YC40_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">carnosine N-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_003669</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carnosine N-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.22" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC40"/>
    <dbReference type="OrthoDB" id="978at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030735">
        <property type="term" value="F:carnosine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012901">
        <property type="entry name" value="CARME"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12303">
        <property type="entry name" value="CARNOSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12303:SF6">
        <property type="entry name" value="CARNOSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07942">
        <property type="entry name" value="CARME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01296">
        <property type="entry name" value="N2227"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4373374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="438" mass="49714" checksum="1912C22F3608CD97" modified="2023-11-08" version="1">MAEKEWEGDFDPMADPEEQHNFFPHLERGADPTSSYRRLAHFNGTHVRRQAFYSMPREHWMLLSKPPFSVLDTFTKMDDLIDSNAELAEAIFMAGFKAFLAPTLDSEWVASIVPEKYAHNEYQIYSIVMDHMKTQTSQADMEKARSCVNQFYREWSAEGAIERSKCFDPVVAALEGEYIRRSHEAAELDKSDLRVLVPGTGLGRLVFDVCRAGFSVEGNEISYHELMASSLILNHTEKVGQFKIAPFALNCSNHIDRSDQFQTFQIPDVHPGSELADVGQSKVPGHERMSMSTGDFCVLYSQPEYAGTFDVVATVFFIDTAPNVIRYIEAVRNCLKPDGIWINLGPLLWHHSSRKEADGNYNEERKHTRADVTDAGIGDPGSVELTAEEVLALVQHFGFAMEKYEYGLETGYISNPHSMLQSTYRPSFWIARKEQSRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC87</accession>
    <name>A0A9W8YC87_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_003390</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="26569at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06174">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013616">
        <property type="entry name" value="Chitin_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF9">
        <property type="entry name" value="CHITIN SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01644">
        <property type="entry name" value="Chitin_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08407">
        <property type="entry name" value="Chitin_synth_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="581"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="612"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="654"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="688"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="732"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="904"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="13">
        <location>
            <begin position="1003"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="13">
        <location>
            <begin position="1029"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="13">
        <location>
            <begin position="1055"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="13">
        <location>
            <begin position="1224"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="13">
        <location>
            <begin position="1253"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="13">
        <location>
            <begin position="1276"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="13">
        <location>
            <begin position="1300"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="13">
        <location>
            <begin position="1321"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="13">
        <location>
            <begin position="1345"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="953"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1393"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="970"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="981"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1400"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1424"/>
            <end position="1434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4373099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1434" mass="158890" checksum="C83151D56B90B618" modified="2023-11-08" version="1">MSGYNRLGGSTGGGDDHYEMDPRRPGRTPSPGQPLRGYQLEDRYASPQPGVLEIPMGPNPAAASGDRLPLQPSYSVENIPYAAGNYHDDYDPRPGGQRHDSDYSLDPQAHHDAYYNQPYDPSPHDESPHPAYGTQPTHYWQDEDDRRPMMPGSNAYGPDPHDADVDPENPFHEGEDPFHDEAAPTPAPAQIKRWKTVKEVQLFKGNLVLDCPIPPRLLNQVPHAQPPERDEFTHMRYSAATCDPADFDAERFTLRQKLFAKPRHTELFIVVTMYNEEDELFARTMTGVMKNIEYMNSRTNSKTWGKDAWKKIVVCVVSDGRAKINPRTRAVLAALGVYQDGIAKQQVNGKDVTAHIYEYTTQMTLDIKKGVVGVKKGNTPVQMLFCLKEKNQKKINSHRWFFQAFGAVLDPNICVLIDAGTKPGKDSIYQLWKAFDLEPMCAGACGEIKAMLVHGKKLLNPLVATQNFEYKMSNILDKPLESAFGFITVLPGAFSAYRYVALQNDKTGQGPLEKYFAGEKMHGANAGIFTANMYLAEDRILCFELVSKRNCHWILQYVKSATGETDVPTTMAEFISQRRRWLNGSFFAAVYALAHSFDIFRSDHSFLRKMMFLVEFIYQTISMLFAWFALGNFFLVFRILTASLQTELGTAGKVLFIIFEWLYIAVLITCFILSLGNRPQGSNRWYMSMVYFWCIIMAYLMFASIYITVRSVQSQIKTNNGFNVADLFKDKIFATLIISLLSTYVMWLVVSIIFLDPWHMFTSFIQYLLMTPTYINILNVYAFCNTHDITWGTKGDDKPEKLPSVTTKADGKADIHAPTDDADLNTQYETELRVFSTKWKEEKKVPSPGEKQEDYYKGFRSGVVLFWMFCNLGLSALVLQAGGLEITVSDPDEAQAKQTQAATIYLAVVLYSVAGLAAFRFIGAMWFLVVRMTIEKSLLAIMAGWDTGAGGGDTWNAEHAGENWGGDAGGAAVNADAGAGDSWGGGGDAGGGAGGGDHGGQTCRICNQTGHFARECPDKPEGGGLTGECYNCGEVGHNKADCTNPRFERPFSGTCNSCGQEGHPARECPTNPMKCKLCDQPGHKALECKSRRIVDWTGVPELESGDAWTALIDASKIKDLDTFRTCLRAYARATLDEFSLPAVEEALREDGLGVYLIAKQQDIAVNMTIIDLIGNPDREYVLSIQLSDKPRRAKLAQGWPENAAQNIERLASAGFVQDKGVPLCGNCGELGHIRKHCKQEQAERESMTPAVQCVYCQEVGHRARDCPKERVNPYACKNCKQEGHVAKECPEPRSAEGVECRKCNETGHFSKDCPNVEARTCRNCGSADHIAKECDQPRNPATVTCRNCDEVGHFSKDCPKPRDYSRVQCRNCNEMGHTEKRCKAPIAEGNGMGDGGPVEAAGGWDTSGDAAATAGGEESSWGAGDTGGGSKFNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCA5</accession>
    <name>A0A9W8YCA5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCA5"/>
    <dbReference type="OrthoDB" id="10250441at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11440">
        <property type="entry name" value="LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="714"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="807"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="714"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="734"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="817"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="948" mass="103309" checksum="A10C1F0D82B9ECF2" modified="2023-11-08" version="1">MPETATDSANHDTHRQNALADIQFASLADEELTPSEDETPEEGRLQLETVQSAIGVSIGEQQTRLTSPKTPAAEKAAALDDADGGVLPDGWDEAVDGPAEVEATANGAAEDADTDRRGWQERSNDDGRRDAQPPTRLPSPWRVGPKTFHERVSDRVNDHRAVLRTHLQNGRRRASSNGSMTEAVRKYMPFNLPASISKTYKDLNFSLPSFSALSLESDRIRPSLGTVNNRRRGTYSNSTPLSSIPQHDGGYSSALQRRSSASRGIMASQHPNTAPIPQRGFSDDSSSTIGPPPLRRESHDQPVSMLRRSNSEGSLLVYRSKSFASSLGDDTRFESVQEQVNSRLKAIRDSWQDSNFKLPSPSFPNFNFSTRDDLFRSRNGTIAPKGRDVEDTNTHRVSFRATIPASASRSTTAMPKRLPVRDGAADSDSYPFFTQALKDLEGDLVILGGYRGSVLRSAEPPNRQVWVPIKVGLNLRKVDLEVPLDPDADERMEETIYPSGMLTHIGPVDISKRLFKRLKACENVKSGKLRVHNYGYDWRLSPHFLSGRMIKFLESLPCNQPGVPKEKRGAVVIAHSLGGLIMRHAINQRPELVAGIVYAGVPQRCVNILGPFRNGDDVLFSSRVLTAQVNFSIRTSYALLPLDGKCFINKYTKEEYPVDFFDVNTWKECRLSPCIAAPFPRPEPTKDSNFSVSGLMSAMSSALPTLASRKSSFSLSKSSQSNNANTSSSTASDAAKDVADVTRNAADKVSEGGAQTAGMEPHMTPNPHPSHPAETSNAPIPASDITIPYDKAISYLTRVLAETKTFKQQLAHHPPHTASNAYPLSRSSTANPRPRFESRDAIKRADVYDELAFASGDGVVLARAAMVPEGYSVVRGGVVSSDRGHVTLLGDLEAVGRCLHAVIHARRGGVGLGLGEKAKEEEKEGIGEGQGREMGGVDGLGMQMPLVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD70</accession>
    <name>A0A9W8YD70_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_003422</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD70"/>
    <dbReference type="OrthoDB" id="5296287at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000313">
        <property type="entry name" value="MFS quinate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF8">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="398"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="428"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="458"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="24"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4373131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="558" mass="61394" checksum="DA36D936DFEEF207" modified="2023-11-08" version="1">MWNPWRENLKDTPKESLNWRLWYGVFVFGLMGAARGIDEGLIGTTAELEPFKKKFGLSDSSKSPHEKAELLSNITSMVQMGSILGALIAFFITDRIGRLWATRQLCVLWVAGITIFLTSAKTGSVGQIYAGRFIAGIGIGQTTVVAPTYLAETAPRAIRGLCVCAFAGAVYIGIMLAYFASWGSSIHISSHTDAQWLVPNSMHLMFAGIIFTLSFFSRESPRWLIKVGRHEEALENLSRLRQLPGDHPYVTSEIIDINDQLNREREATMGTSWLGPIRELFSSKANLYRIMLSLMSQLLAQWSGANSITIYAPQYFAMMGTSGQNEKLFATAIFGVVKFISSMFCAFFLIDYIGRKRSLSAGISLQLFAMLYMAIFLLVDNGVSDKSKPQTSSQKHAAMGAIVMIYFSGFGWALGWNSIQYLINSEIYPLRLRALGGSIAMTFHFVNQYGNSKAVPLMFLAMTTGGTMLFFSCVTAIGLAWVWFFLPETTGKSLEAMDEMFSLPWYLIGRKGAELTRGSGGLSEVLDHAGEKAATVEMENVGGGAAVPGAQGQRVEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDB9</accession>
    <name>A0A9W8YDB9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 19">Squalene synthase</fullName>
            <shortName evidence="19">SQS</shortName>
            <shortName evidence="19">SS</shortName>
            <ecNumber evidence="4 19">2.5.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ERG9</name>
        <name evidence="21" type="ORF">N0V83_002740</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Squalene synthase; part of the gene cluster that mediates the biosynthesis of squalestatin S1 (SQS1, also known as zaragozic acid A), a heavily oxidized fungal polyketide that offers potent cholesterol lowering activity by targeting squalene synthase (SS). Catalyzes the condensation of 2 two farnesyl pyrophosphate moieties to form squalene. The presence of a gene encoding a squalene synthase supports the identification of the cluster as being responsible for SQS1 production and suggests a likely mechanism for self-resistance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 19">
            <text>2 (2E,6E)-farnesyl diphosphate + NADH + H(+) = squalene + 2 diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:32299"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15440"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 19">
            <text>2 (2E,6E)-farnesyl diphosphate + NADPH + H(+) = squalene + 2 diphosphate + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:32295"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15440"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="19">Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the phytoene/squalene synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.21" evidence="4 19"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDB9"/>
    <dbReference type="OrthoDB" id="431150at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051996">
        <property type="term" value="F:squalene synthase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045338">
        <property type="term" value="P:farnesyl diphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00683">
        <property type="entry name" value="Trans_IPPS_HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000003">
        <property type="entry name" value="Farnesyl-diphosphate farnesyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006449">
        <property type="entry name" value="Squal_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019845">
        <property type="entry name" value="Squalene/phytoene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044844">
        <property type="entry name" value="Trans_IPPS_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033904">
        <property type="entry name" value="Trans_IPPS_HH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01559">
        <property type="entry name" value="squal_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11626">
        <property type="entry name" value="FARNESYL-DIPHOSPHATE FARNESYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11626:SF2">
        <property type="entry name" value="SQUALENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01018">
        <property type="entry name" value="Squalene/Phytoene_Synthase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01044">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01045">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="10" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="13" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="6 19 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="417"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00053206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU368088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ4374001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="482" mass="55607" checksum="9259DBAD5FE6994A" modified="2023-11-08" version="1">MPSPMSILYYVFHPNQLRSIIQWKVWHNPVHERDESKECESLKRCFYFLNMTSRSFAAVILELHPELLVPVALFYLVLRGLDTVEDDMTIPLEKKEPILRNFQNVLEQDGWNFAENGPNEKDRQLLVEFNVVIEEFKKIKPAYREIIKDITKRMGNGMADYANNAEHNLNGVNTIKDYELYCHYVAGLVGEGLTRLFVEAGLANAALLKKPELMESMGQFLQQVNITRDIKEDLDDGRRFWPKEIWSKHVDKFEDLFKPENKDKALNASSEMILTALTRADDCLYYLAGLREQSVFNFCAIPQSMAIATLEACFQNYAIFQKNVKITKGDACQLMVESSQNLQLVCEIFRRYARKIHKKNNPHDPNFLKISIALGRIEQFIESIFPSQKPPVDQTSPASVEEAERKKKEDDEAKWDLIYMITAVALTVGIITFFMLFVAWLAGARFDIAYYQFKEQMGELLGYNTKPAQVTEVHVASDHSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDH9</accession>
    <name>A0A9W8YDH9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pyridoxamine phosphate oxidase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDH9"/>
    <dbReference type="OrthoDB" id="539398at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39336">
        <property type="entry name" value="PYRIDOXAMINE PHOSPHATE OXIDASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G11440)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39336:SF1">
        <property type="entry name" value="PYRIDOXAMINE PHOSPHATE OXIDASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G11440)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="32040" checksum="BACB93D389E0C17E" modified="2023-11-08" version="1">MVKFYPSLNESHVEFMTNQPIFYVASAPWAGDHINISPKGHPSRTFSVLNPTTVAYLDATGSGCETISHVYENGRVTLMFSSSGPSPKVLRLFCKGRVVEKWDPHYNQLRAKMATENGDEVDMTGARAIIVLRVKKVQTSCGFTVSEGAADAVAMVDHEVDTDAAHELAHRETLDDWARKQVEKKELGDYQKRMNHRSLDGLPGMMSARRSHDENIAVEDAKAWFRKVSRQQDAVAFGIGLGILFMIVLSLGGVLSIRPVFIEHILNFQRRQMGIPEEPVWNKQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE13</accession>
    <name>A0A9W8YE13_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Eukaryotic translation initiation factor eIF-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SUI1_1</name>
        <name evidence="10" type="ORF">N0V83_002622</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Additional factor that functions in concert with eIF-2 and the initiator tRNA in directing the ribosome to the proper start site of translation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SUI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE13"/>
    <dbReference type="OrthoDB" id="10248435at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045048">
        <property type="term" value="P:protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11566">
        <property type="entry name" value="eIF1_SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000005">
        <property type="entry name" value="Sui1 translation initiation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005351">
        <property type="entry name" value="ASTER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005874">
        <property type="entry name" value="SUI1_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01160">
        <property type="entry name" value="SUI1_MOF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10388">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03669">
        <property type="entry name" value="ASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="SUI1" evidence="9">
        <location>
            <begin position="125"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4373883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="210" mass="23511" checksum="6679C91978D616BA" modified="2023-11-08" version="1">MASKKDMRRADLIVPYAEPAKDKNENDMSSTMASTLPMAAIFTRNKMIGWVAVVFAIQSWLAETPEQKRTATTPAYFQVGMSAMSLLVSAPTSSRMSIENLKTFDPFAEADEDTGQVKQSQQDYIHIRIQQRNGRKTLTTVQGLPKKFDQKKILKVIKKKFACNGTIVADTEMGEVIQLQGDQRKDVQDFMTDKKEGLGLDAKTIKVHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE46</accession>
    <name>A0A9W8YE46_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Indoleamine 2,3-dioxygenase</fullName>
            <ecNumber evidence="5">1.13.11.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_001832</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Produces N-formyl-kynurenine through the oxidation of tryptophan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-tryptophan + O2 = N-formyl-L-kynurenine</text>
            <dbReference type="Rhea" id="RHEA:24536"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58629"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the indoleamine 2,3-dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.52" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE46"/>
    <dbReference type="OrthoDB" id="540174at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033754">
        <property type="term" value="F:indoleamine 2,3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000898">
        <property type="entry name" value="Indolamine_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037217">
        <property type="entry name" value="Trp/Indoleamine_2_3_dOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28657">
        <property type="entry name" value="INDOLEAMINE 2,3-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28657:SF5">
        <property type="entry name" value="INDOLEAMINE 2,3-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01231">
        <property type="entry name" value="IDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140959">
        <property type="entry name" value="Indolic compounds 2,3-dioxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00876">
        <property type="entry name" value="IDO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="4 5" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="proximal binding residue" evidence="4">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600898-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4374756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="419" mass="46518" checksum="80E72D1BD2703788" modified="2023-11-08" version="1">MSPHKIDVLPSPAALPQYGISKNGFLPPEAPLARLPQNYYEPWERIIQQLPELLNAQQIRPWVDRLPILSTRHLGGEPEWQRAHSMLAVIAQGYIWCGPEPSERLPPAITIPLLATSEHLEVHPVATYASLNLWNWRPLEEGVDITQPDNMVALQTATGSDDESWFLIISNAMEARAGPLIETMLAAMEAVEVEDFPKIVGALNYFKQSMEDIGTLLERMDERCNPQIFYHTIRPFLAGSMNMEAAGLPNGVFYDEGNGKGSWRKYRGGSNGQSSLLQFCDAVLSVDHARSGGFHKEMRLYMPGPHARFLDEVEAIANIRGYVDSHQDNIELLGAFNEAVAALSRFRDKHIALVTRYIIIPSRMGKPTGAIDRRDIASASTMLASQKPQTQELIGTGGTTLIPFLRTSRDETSAAAVTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEC6</accession>
    <name>A0A9W8YEC6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002802</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEC6"/>
    <dbReference type="OrthoDB" id="3801155at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="314" mass="33482" checksum="6E6ABFAA3443B649" modified="2023-11-08" version="1">MSRPLTTLGNRLRTATISLPIINRTPQRPFSFDTATSKARAAQWEPNLPSLDALLSVKQQQQDDVDDQDGGEKDQDGDLPMMTTYGAFDPGASRPLMLHSGVGVAVPSIADVYLRDVVPGGDRGRGRAITVGEKAVTIKDKVVTTTATTTTAVTLPNKPWPYRLPAFLQPGVGTTFEMMHHATTTTTTLTPTTSTAAAPAPASHESNIHGYDKAPQRIRISILQAILYGVPEPEKFSFAKTVVREGGVVHKILYGVKDHEGTCSGGVGVAVAVGKVVGVAETETETETEIAGEEARVVVDEKTEKIEEVVVVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEN2</accession>
    <name>A0A9W8YEN2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamate-5-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="2">1.2.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Glutamate-5-semialdehyde dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Glutamyl-gamma-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PRO2</name>
        <name evidence="13" type="ORF">N0V83_001087</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19541"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the gamma-glutamyl phosphate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.41" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEN2"/>
    <dbReference type="OrthoDB" id="1934954at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004350">
        <property type="term" value="F:glutamate-5-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07079">
        <property type="entry name" value="ALDH_F18-19_ProA-GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000006">
        <property type="entry name" value="Gamma-glutamyl phosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00412">
        <property type="entry name" value="ProA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020593">
        <property type="entry name" value="G-glutamylP_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000965">
        <property type="entry name" value="GPR_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001221">
        <property type="entry name" value="PRK00197.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00407">
        <property type="entry name" value="proA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063:SF8">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063">
        <property type="entry name" value="GLUTAMATE SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01223">
        <property type="entry name" value="PROA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="12">
        <location>
            <begin position="11"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4375810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="476" mass="50851" checksum="563AC566DB2F665C" modified="2023-11-08" version="1">MSLTNATPLEVAQAARLGSRKLAVLSTEERNAALTAIYTALADGKDEVLAANAKDLEAANKAAAGGTLSASLVKRLDLGKPGKYEDMLQGILDVRELEDPINRTTAKTLLDDNLTLSRITCPIGVLLIIFEARPEVIANISALAIKSGNAAILKGGRESSSSFAAIASIISKALASTAVPSDCLQLVQTHDVIADLLKQDAYIDLCIPRGGNKLVRYVKDTTTIPVLGHADGICSTYLCADYPVEKATKIIIDAKTSYPAGCNALEQLLVEESALSTTLPSIAEALLQKGVSLRCDPASLSALKARLDPHSAALLQEAGPEDFDTEFLDYILAIKTVATLDEAISHINAHSSHHTDAILTLSESTAKSFLAAIDSSSVYWNTSTRMADGQRYGFGTEVGISTNKIHSRGPVGLEGLTIYKWVIVGDAQVSAEYGAGGKAWKHQVLPVDSSSEQEFVARDEQEREILRRFRAQRAYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEX9</accession>
    <name>A0A9W8YEX9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Two-component response regulator SSK1p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MgSsk1</name>
        <name evidence="7" type="ORF">N0V83_001268</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the SSK1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEX9"/>
    <dbReference type="OrthoDB" id="21225at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000146">
        <property type="entry name" value="Putative two-component response regulator SSK1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="542"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="153"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="413"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="733"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="18"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="427"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="470"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="491"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="738"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="759"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="788"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00093463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4375988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="802" mass="86981" checksum="04C0C3472DDFD79C" modified="2023-11-08" version="1">MSSIKTRLQNARSKFLRRSSATSTATRSITSSAGSERQYHQQQQQHLASTIRKSMSLSKLPEMSSTDVPDDSPPGEQRLVRSEVLGTGVTESRAEPDGSLTSRDGHSRQSAYTRSSTNTTSSARPPSPVVPADVPRACPELTLEEPTPIHPAVQPAELDRSGPAPSLSLSLSSAASSEPTPSDAEPPSPRRASTSARLTPPGIVRRQSLIANTDAKIIKTLLAPDIPQAAIATGPPTSTDYFGGAPTNLAGMLHRKIWVKRPNASATLVQIREDDLVDDVRDMILKKYANSLGRSFDSPDVTLRIVPRDAQKGQDRTLGPDEDMCRTLDAYFPGGQSVNEALIIDVPQKRTPKASPRIPLYYSHHDDSVNHQTEYFPPMPVINPSPAALGGGQHDARIPLPHAPHSIAVLNTGQLPALPSPGGTRRGPLHAHRPKFPRHNTASPTALTGGIPSSATTSRPPNRPRHDSSASEAKNSATSQNPIPTPPASTDPITRQQTLQTSNPPTPRVSSPQPNKRRKKRQPLEPPSLPAGLLDGSVPPINVLIVEDNIINLRVLGAFMQRLKVRWQRAMNGKEAVTKWKAGGFHLVLMDIQLPVMNGLEATKEIRRLERVNSIGVFSSGSSEAPNQRLGVEKQDELIEGDRLGDKGLFKSPVIIVALTASSLQSDRHEALAAGCNDFLTKPVNFVWLERKVKEWGCMQALIDFDGWRRWKDFADKSENNDSTSKLSVSYHSMAPSKKTTSISPTLSAKSPDSANGVKNDDDAKEKERERKSKRRSMGVIPPPALPEEVETPPPETDDGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF13</accession>
    <name>A0A9W8YF13_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">DNA replication complex GINS protein SLD5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SLD5</name>
        <name evidence="9" type="ORF">N0V83_002275</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The GINS complex plays an essential role in the initiation of DNA replication.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GINS4/SLD5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF13"/>
    <dbReference type="OrthoDB" id="338231at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11711">
        <property type="entry name" value="GINS_A_Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21692">
        <property type="entry name" value="GINS_B_Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008591">
        <property type="entry name" value="GINS_Sld5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031633">
        <property type="entry name" value="SLD5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038749">
        <property type="entry name" value="Sld5_GINS_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21206:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN SLD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21206">
        <property type="entry name" value="SLD5 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16922">
        <property type="entry name" value="SLD5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007764">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GINS subunit" evidence="7">
        <location>
            <begin position="56"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="DNA replication complex GINS protein SLD5 C-terminal" evidence="8">
        <location>
            <begin position="169"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR007764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4375191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="225" mass="25772" checksum="55E11266020AB9A2" modified="2023-11-08" version="1">MDIDDILAEAAVDSTPQEAYDLQELTRSWVAERVAPEILSWPGDLMDRVLKAIRRQIELVEDQTGNMDPKTNFRLIIYQTELERFKFLVRSFLRARIKKIDMHPLHIQAQHAASLETLTPLLSPSEYQYLTSHQTLLSSHYSSSFLGQFPVSLQRLDDTTGGISMIDRPDEDKAVFIRALRDVGEVFVEGTDRRFEIKRGDVWVVRWSAIRPWAVGTGTGDVELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG31</accession>
    <name>A0A9W8YG31_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001943</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG31"/>
    <dbReference type="OrthoDB" id="3792443at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4374865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="198" mass="22035" checksum="EE3A21061E8873C6" modified="2023-11-08" version="1">MEETIAAVIEGAVIIVYTSENTKYLRAFLDRAPGRYGVVRQLNFNSFSRCSTNVPVNQDLELAVRCTGLRTITLILDDENLTLDVFSGDGTSDDGTSDGPVLIPMKPEDLYANFELERLLDCNLLSKIIIEHCNYTSVAEDVVIELGQMLRQAITKKGTRQTVEILRHYGSSTHGYGLINDYQKLIGTDLALVGDNWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG83</accession>
    <name>A0A9W8YG83_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF962-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG83"/>
    <dbReference type="OrthoDB" id="2124888at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046521">
        <property type="term" value="P:sphingoid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009305">
        <property type="entry name" value="Mpo1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28026:SF9">
        <property type="entry name" value="2-HYDROXY-PALMITIC ACID DIOXYGENASE MPO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28026">
        <property type="entry name" value="DUF962 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G05310)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06127">
        <property type="entry name" value="Mpo1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="21101" checksum="A0BC13C8FE32AA43" modified="2023-11-08" version="1">MPVFDLKKNLVFYGAYHRDPANVAIHIACVPILLATGFLFGTNTPTLPIRQPTLLTRLNLPLNLGTLAAATYSTLYLLLSPNLAGVSIAPLILGAASLANRLTTKYNTTKVNTIAIAVHVISWILQFIGHGKYEGRKPALLDNLVQALFLAPLFVWYEVLFKLGFYKELRRDVEGAIEIEVAKLKGKGKEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGF4</accession>
    <name>A0A9W8YGF4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000282</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGF4"/>
    <dbReference type="OrthoDB" id="3546893at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040733345" evidence="2">
        <location>
            <begin position="16"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="698"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="816"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="883"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="996"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="441"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="515"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="531"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="698"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="709"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="834"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1014"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1054"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1087"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1103"/>
            <end position="1114"/>
        </location>
    </feature>
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        <location>
            <begin position="1115"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1131"/>
            <end position="1149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1192" mass="128643" checksum="9AACC5F18085353A" modified="2023-11-08" version="1">MLCLLVALIFLLVRAVKKHKQLLADLEERGVSIAQAQKEAGGDSVTRPRTVLRRNTVLPFNKSGWGTLPSVETFRSAESTNVPAHYVPPKPTEELKRNSRLSWPFSARRLSGHSSHSIHMKKIKVSRLSTVIEDPKASPMAHVLTNPHLSASRSSVTLSGLNGGPASSTQSLLQYHPAFRHQGQESATPAPTIRANNGSITHTDPNGRLQRAKSVAAVPSLQPSRPQLRARSISLCSQTSGIAPDVILPPLPLDIARIKSDAKRRSQLHRAPSKLSISSFGSADTSILTSRSPVVPQTSNVTRKITKPNAKGSNFARTKPFRDTLDLRTKVLGGRSSSQNSPARISMVREEQGEGQVDNQGTLSKESSTNSLGTIKGAQAVTMSKFSSPTASPLSAPNSTTPKRKLKTQISSSGSPERQYQSSTASRSLAGAMRSPKRQHSQTSSRSSGGNPFQWDPTPLSSAAKPSALKGSPSARQGHRRKNSVRISLVPTIHGPPSRPPSLSISVDNKEDTTEGASARSSKTGADLGISNTRSLPTPPSSSTFAPDLKFSATSLRASLTPTSPELPLVGYDQSYVVLPTDQVLPPLSAVELKRLSNGSLFSLSQFPTAPSIIEPMDFDMSYPHIYPIAQPYDFDGNWMPDTPLIQQYPFHAHMSDRDRSPSPIDIDEYNPEQPNCIYQTPMNTNPRSFQSAFATIPEESSVSSNRTVDLEHSRYDDSPPVSPKTMSPPRFNLMDPSVYNLPTHATVILEEPLDTIDPAVLSKDAFNMLNGNYSNAEGSIFKTPNSNRSSIAIPTTPGSARSMFDPLLEAALPSSPPNAYMDSPTLAHLGTQSPSSSIYSSPSPALSLSSWPADLPSPAIPCSPRPGHTQLPTSRLSINFAEMPKLKPSPRGPRGSPPRPLRSSIAALRRMNSDVMDAEKEKTGRGERRYLRLGRGDSLQLPGDESWLDEMEDDDDDAVELDDVEGRRLVGNVLEGWEDEGCTVLDLDEGNTTMLSNSTIKPESEQAKAKAAGTNTTTGMNNISTSSIWEDGEKFWASNTPPQPRSSGSSPSNNLMSQYQPLASSPVLPQTKPTPPSTPTSKPSSRKRDFHVAKDDAPPSPTENSTLNTSPTSEHNKKSRDSFNDTNSYNNAATTSSGGSGRRSNTSGRSHRKRRVLGVGTPNVRIQVTSPGGQVRGTPGSLYDQQGFLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGU3</accession>
    <name>A0A9W8YGU3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_000543</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGU3"/>
    <dbReference type="OrthoDB" id="3063237at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15853">
        <property type="entry name" value="SNARE_Bet1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039899">
        <property type="entry name" value="BET1_SNARE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4377714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="97" mass="10713" checksum="26294C5A15E6C0F7" modified="2023-11-08" version="1">MSQAYEREQQNNSRLDELASKVSALRGVTIDIYDSARDQHVIDSTSETFSSFGTTLKGSATRLTRMAQSGNKVAILKLAGMIIGVVVVLYWIWGLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGW8</accession>
    <name>A0A9W8YGW8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">5'-nucleotidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_000541</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the 5'-nucleotidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGW8"/>
    <dbReference type="OrthoDB" id="7722975at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009166">
        <property type="term" value="P:nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07409">
        <property type="entry name" value="MPP_CD73_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000020">
        <property type="entry name" value="NT5E isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.780.10">
        <property type="entry name" value="5'-Nucleotidase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008334">
        <property type="entry name" value="5'-Nucleotdase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036907">
        <property type="entry name" value="5'-Nucleotdase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006146">
        <property type="entry name" value="5'-Nucleotdase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006179">
        <property type="entry name" value="5_nucleotidase/apyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575:SF24">
        <property type="entry name" value="5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575">
        <property type="entry name" value="5'-NUCLEOTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02872">
        <property type="entry name" value="5_nucleotid_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01607">
        <property type="entry name" value="APYRASEFAMLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55816">
        <property type="entry name" value="5'-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00786">
        <property type="entry name" value="5_NUCLEOTIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041019403" description="5'-nucleotidase" evidence="6">
        <location>
            <begin position="22"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="566"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="9">
        <location>
            <begin position="44"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="5'-Nucleotidase C-terminal" evidence="10">
        <location>
            <begin position="343"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="531"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="540"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4377712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="589" mass="63962" checksum="E5073BC5BDBFD6CE" modified="2023-11-08" version="1">MKFLARSSVIALSALAALSRAEEALYSRRLAKRGIDAQGNYNISFFHVNDVHAHLDEFSSSGTDCTKPERGCYGGYSRIKTVVEEQRPNYNDSLWLNVGDEFQGTLFYSFYGGEKIAETLNQMDFDAMTLGNHEFDGGDEKLGQFLENLTFPIISANVHSQNEKVNKSVKPYHIFQEYGLALIGVTTNETKSISNPDDSTTFSDVVEAVQSAVDEIKATTNITRIAAITHIGYDKDQELAQAVSGLQLIMGGHSHTLLGDMEDAKGKYPTIVDNKDGDEVFIVTAYRWGEYVGYIDVTYDTEGKILAYHGAPIHLTNTTEQDADLQAQIEAWRGPFEEFAAQVLGESEVELDQTTCQEKECLLGDFMADAMLAYRKNNTDDVDFAIINAGGIRVTIDEGEITRGEVLTSFPFGNAIVEIPVSGRELWEVLEGIVTGVNVANGKEVTSFFQISSGIKVEYNPALNNGSKLVSITIGDKPLSNDTTYNVVTLDFIAGGGDNFFTPKTEFVVLDTQDEVLVQYIQAQSPVNMKLDGRISETDGQSGSGNSTSNGTSPTSSGTPPQQTGAAIRLGSGGIGASIFGILVGLLML</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHC7</accession>
    <name>A0A9W8YHC7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Pre-mRNA-splicing factor slt11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SLT11</name>
        <name evidence="13" type="ORF">N0V83_000947</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in pre-mRNA splicing. Facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome. Binds to RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLT11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHC7"/>
    <dbReference type="OrthoDB" id="10259600at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036002">
        <property type="term" value="F:pre-mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12265">
        <property type="entry name" value="RRM_SLT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000396">
        <property type="entry name" value="Putative Pre-mRNA-splicing factor slt11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034356">
        <property type="entry name" value="Slt11_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048995">
        <property type="entry name" value="STL11/RBM22-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF6">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21369">
        <property type="entry name" value="STL11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="235"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="155"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="331"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="117"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="331"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4378115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="375" mass="41171" checksum="D453A48B504FDA58" modified="2023-11-08" version="1">MPPQIKQDLNRSGWETTDFPSVCEKCLPDNPYVQMLKEDHGAECKICTRPFTIFRWKADRTARQKRTNICLTCARLKNCCQCCMLDLSFGLPIVVRDAALKMVAPGPQSDINRQFYAQEHEKEIEEGRGAMEAYEKTDEKARELLKRLAQSEPYYKKQRRIEAETEESGQKALPAPGGSGSSTGGHVPGPIRTRDSRGTPGARGSMRPGRGGRVGGPSAEPSPQDWLPPSDPNVVSLFVTGVEDDLPEHDIRTHFAQYGTLRSLVCSHRAHCAYVNYAKRKDAETAAEALKGKVVIKGCPMRVTWGKPKQLDSMDQNVRMQYAKEGRQVAGSARRAAASQAAIEAAPQNSLDSMAPIAPPPGADDEVTYASLAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHE8</accession>
    <name>A0A9W8YHE8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_002403</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHE8"/>
    <dbReference type="OrthoDB" id="19981at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021013">
        <property type="entry name" value="ATPase_Vma12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31394">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31394:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11712">
        <property type="entry name" value="Vma12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="20"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4375317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="285" mass="31445" checksum="81DBCA34353E2877" modified="2023-11-08" version="1">MVLLTMTPGIARAVARAAELSSDDLAKLQQPDEPALADPKPGNPISHSQLIDLSKLLKQHSSTLPKNPKQDTTKGPSSTTDQNDHQQEEPLPQTLAALLHTTTLYTPPPPAKKPQTPEYTALMARLRAQQEAQSYQRMLHPPPTRESFTQRFPHAAQPFSLGATAPTDEEEVSYEEVHRQIILIINVIVSIVAVAVFIWVAARHWSVGKRLGLSMGGSGAIAIAEVAVYSGYVRKVKEAKRAEKKKPEIKEIVKSWVFEKDEGSGVTGFKERADDGVRFRKGKHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHF4</accession>
    <name>A0A9W8YHF4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="1">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_000993</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHF4"/>
    <dbReference type="OrthoDB" id="2248014at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02662">
        <property type="entry name" value="Peptidase_C19F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF904">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="4">
        <location>
            <begin position="45"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="208"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="455"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="221"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="491"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4378160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="616" mass="68085" checksum="C998465D1E05AC9D" modified="2023-11-08" version="1">MPEKPLTIATYAAGASLAAITLVYVFGPTFFLDDDAASNKKKGVVGLSNAANDCFINSVLQALAGLPDLRIYLIRETHRRKLDGPEVYHVDPARHNTGTTAIKPGKLEGLQRGLLTTGLKEILDSLNERPIYKKTISPQPFIRALERAFGTRISRQQQDAQEFLQIVTERLCDEYHAGTKARRQAKRIGIAITNGNTTPERREIVEQFGVDPAQVSSSENRDEDQEEEDEPLTDEEGFPFEGKIESQIECQTCHFKPKPSVSTFVTLTLNVPQVSSTSLNACFDGMLKVEYIDDFKCEQCRLEHAIKSKNQELSRATDPGLKERLQSDIAKIQQALEEDPENPPLEVELPASSQAPKRRIARHMYISQFPKVLAIHLSRSVFAVGSASTKNLAKVAFPESLPLGGLLHRKNYRLLGLVSHKGSHNSGHYESFRRQVQPLPFSTPVSFGAEGAYSRQASPYPSAVPSAVPSAVQSPRISTTNLTGTHDRGSPNPSTLALDSPSTQSISSHDSLGSHATSRGPPPTSAPRQPETDSRRPPSSSPDPELSRTRSLRSLRDKATETASSIAESNPLRRRSKKAINRWWRISDDKIKESKTSEVLGMAKEVYLLFYELDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHJ7</accession>
    <name>A0A9W8YHJ7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000579</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHJ7"/>
    <dbReference type="OrthoDB" id="6359943at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47369">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47369:SF1">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="211"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="523"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="752"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="825"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="535"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="975" mass="107773" checksum="6B751B20FEA623CA" modified="2023-11-08" version="1">MDSANRPLSSHDGDGMDHEGQGQLGDPNNGETWALVDSEVADSAGPSITTPVSLFSPGIPVSQPSPSPSFASANSRVHVDNCSPAGSSRSLPSLANITGMAKRTARLKMSDEPTPIVQLHTQIEQAQKSADDPTTPLDSDSSRRTTLDLMRSEHTTRTDINRLFSEGANMTGSRAPSYNNATGPLYLRASSEAIPLPDHLFTRGLLEGRHSDITVIAFGQRYNLHRLILDRAPFFTTALSEPWIESRSKEVALHPEEIDHNITQTSFELAIRKLYGVDISKESDYEAVGLFATACWLEMQDLIDSAIESILRQMAPDTLAYLIRLVTTNYYGRSGDRILESAKAMLSRNGWEMPIKYWDDMPGDIIRELIGSDGFFVDSEWDRWVLSKRILDRRLRHAAIEAGLMKRGQRPIPQAPNSLRLMAVRFDGVYRQNALTAGNSSSDTHADWLALYTTPDIERMLVLLDEGIHYIHMEYEQLDFIRNSRDVFGLPVMPERVISNALWQQLALRQRVLNTDESAQELGLCQSPPEPGVSNIATKTGGNSLTKGKSKAVVPAQEDTEIESESWDGNGRPRKFWIPSSDCNIVLGNGADPVITTSNSFQRHASRLSATIQPEDAQWATDFASTPSTLTNPSTRMHDTQRPISAGGGNAGQPKPIAYTEFPPFRFSAEFPNPRFLKEKKRVYSRTVSYAGSLWNIYIQKVRSAKNVQLGVYLHRAKEREVEEAAHSSGLSQQNNGSVDERIGQLEREMLMRSERRDRRRSTRAHFNDPNAEEDTSGSGGDPDATLVSPGLRNPLFSSSSLGRRRSANVPRLSSRGIPQWDFQALATSPPPINENTDSSSFPLAMYSPSDHGYDSSDDDETDAAAYPNPSYALRAQRKARALVPTLPPYVDGRPTIKTYFKIYSPSKGGRMLSIYESAPDRFNFSQSWGWKSSTLMLDETTISGGGEDAPGGANPAASKKTDGKLRFMVVIGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHN6</accession>
    <name>A0A9W8YHN6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pyruvate dehydrogenase E1 component subunit beta</fullName>
            <ecNumber evidence="10">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PDB1</name>
        <name evidence="12" type="ORF">N0V83_000949</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="10"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHN6"/>
    <dbReference type="OrthoDB" id="10266385at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07036">
        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000006">
        <property type="entry name" value="Pyruvate dehydrogenase E1 component subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027110">
        <property type="entry name" value="PDHB_mito-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006667">
        <property type="entry name" value="PRK09212.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008854">
        <property type="entry name" value="PRK11892.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624">
        <property type="entry name" value="DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624:SF96">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 10 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="9 10 12" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="11">
        <location>
            <begin position="48"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4378117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="374" mass="40398" checksum="8EE145F91C4D382F" modified="2023-11-08" version="1">MAASRLLRPASRLLSASRPAPAFRPAFRAAAITPSIARRGYASGQKEMTVREALNEAMAEEMEANDKVFVLGEEVAQYNGAYKVTKGLLDRFGERRVIDSPITESGFAGLTVGAALAGLHPVCEFMTFNFAMQAIDQIINSAAKTHYMSGGIQPCNITFRGPNGFASGVAAQHSQDFSAWYGSIPGLKVVTPYSAEDAKGLLKAAIRDPNPVVVLENELLYGLSFPMSEEAQKDDFVIPFGKAKIERPGKDLTIVTLSRCVGQSLTAAEQLKSKYGIEAEVINLRSIKPLDVEAIVKSVKKTGHMLCVESGFPSFGVGAEIMALTCEYAFDYLEAPPARVTGAEVPTPYAQKLEEMSFPSEQLIVEYAAKLLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YI73</accession>
    <name>A0A9W8YI73_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_001604</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YI73"/>
    <dbReference type="OrthoDB" id="2123952at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF6">
        <property type="entry name" value="PROLINE UTILIZATION TRANS-ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="4">
        <location>
            <begin position="3"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4376321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="376" mass="42760" checksum="145666B9E9EF0E5F" modified="2023-11-08" version="1">MYQCYFPFLPENDLLVRWQDKIPTPDEPSYVLLMALCAVSAQTAALNAVFDNTLLENLSIPDGELYFSEAVSMIPHRIAQSEMHSLDYLRSFGLLAVYSLQRGNHNDLHRYLGLYHALVAQQGFHDESRWPTDLSLLDIDDRRRLFWCVYRLEIHSACVLGHIVRMPESQVSVQYPRITPAMEPETQAWTAGWDYITDLFRLMEYAIFSLRGAKNRKAVLAVFCERPSPTTLLDALARLKSSKPRILLRLAEPESAFQSNRCKYMAVQITCTETLVNIMALLYCEAPACEIINITEDFLEEVAKAPLIMFKVAGSQIVHQLLGVGHMLFNASKYENGRYWSEAKRLIEFLGDLVTNLEDDIPSAAEAGSRLLRLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YI93</accession>
    <name>A0A9W8YI93_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">60S ribosomal protein L28</fullName>
            <ecNumber evidence="6">3.6.4.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RPL28_1</name>
        <name evidence="6" type="ORF">N0V83_000223</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YI93"/>
    <dbReference type="OrthoDB" id="61900at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_uL15_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="5">
        <location>
            <begin position="73"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4377398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="150" mass="16643" checksum="C47B05FE33B764B8" modified="2023-11-08" version="1">MDALASTANIPEVYVIENSDDYRLMTLTSHALRGLAGGQHHHRTNLDKYHPGYFGKVGMRHFHLQRNHEWKPVVSIDKLLSLIPAEHRGKYLDSSSAPNTAPVINLLNHGYAKLLGKGRITIPVVVQARYISAEAERKMKDAGGVVQLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIB8</accession>
    <name>A0A9W8YIB8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_001424</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIB8"/>
    <dbReference type="OrthoDB" id="5417887at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF147">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="38"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4376143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="361" mass="41222" checksum="37DABF1A4260D0C5" modified="2023-11-08" version="1">MISRRKNDEPDPAYVDPTVQLNAGLWTLFAGASVFLALRLWIKFTRGHGLWWDDHILLVTWAILLANNSIISTEYATGYVTASWDDRMHILINITSCGTLIGQALSKTAFAVTLLKLTKGWQRWILWFCIGSLNLYMVVKVILQWAKICGKPSYDVWYRLDICLNSKFRDDFKEGGNVYNIIMDFVLASFPWFITWKLDMRRVEKIGLNVTMSLGMVVAIIAAVRTSWKDEGNQKDALYFWRNAHSNIWYSSEIVGTIIVQCIPVMRPLLRDIHMSLTSKRLASAAENTARRSKMPVAYGGNINSHKAHIYSDAKSNTYNDAKNETDVDSWDSQGIFQKLDFELTTMELKPEKGEKNTSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJ47</accession>
    <name>A0A9W8YJ47_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Quinate repressor protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_000693</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the 2nd section; belongs to the type-I 3-dehydroquinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the shikimate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJ47"/>
    <dbReference type="OrthoDB" id="4415835at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00502">
        <property type="entry name" value="DHQase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01065">
        <property type="entry name" value="NAD_bind_Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000019">
        <property type="entry name" value="Quinate pathway repressor protein QutR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000386">
        <property type="entry name" value="Quinate repressor protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001381">
        <property type="entry name" value="DHquinase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041121">
        <property type="entry name" value="SDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090">
        <property type="entry name" value="AROM/DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090:SF27">
        <property type="entry name" value="QUINATE REPRESSOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01487">
        <property type="entry name" value="DHquinase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18317">
        <property type="entry name" value="SDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01100">
        <property type="entry name" value="SHIKIMTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0672">Quinate metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Shikimate dehydrogenase substrate binding N-terminal" evidence="9">
        <location>
            <begin position="528"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase" evidence="8">
        <location>
            <begin position="670"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="domain" description="SDH C-terminal" evidence="10">
        <location>
            <begin position="816"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4377863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="873" mass="96398" checksum="37824CC935635A5E" modified="2023-11-08" version="1">MSQDNMASNTFSHAGQLPLSSGATPPGTSGSYDRQNVSVGGIPSVGSSARSTPRDTPAPTAESNECRRRFEPDASIVLIGMRGTGKSTLAVIASIACRRRVVDIDDLFQEATGFYTAKYRKQFGAANHNLRQEELLRSALQTHNKGAIIVCNGGSLERNGQALMRDFAKTHAVIHVVRDFHSMHEYLNGVELSRLKDLLAFTAPILRRCSNYEFYNMSETTTATSTTTTDELAAPAFLTLKRTQRTFLKFLSLITTREHTGGPVGSSIPALEPGYPLSDVPTELRKYTCAVQVPLADLLAEDVNIQCFELGSDAFEVTVDPNRHRLTTDHVDDISSCISKVRRSTVVPIIYHVLPTVESPVSYLEHVHHGLRMAPEFATVDLSLDHAKLTEVITSRGITKIIGTLHADKPWDDPSWIMQYDLAVRLGCAVVRFTRPARSTDDDVPTQSLQSRVSTSEIQIPLVLYNTGCAGRRSVCFNRHLTPVVPSLLREKSAFATPLEPNTSWITAREVTKALYTSFTFDPMNMYIIGARLGYSVSPAMHNAAYDSCGMPHHFVRVQSPSLNSLKELVRKPNFGGSIVIQPFKIEVISLADSLSQHARAIGAVNTLIPVRHLQGDGTIPSDLELFQERNQAGPIQALYGDNTEWIGIRSCVRRGLTPANAVRPTTSGLIIGAGGMARAAVYALLQLGVKHIVIFNRTLEKAQNLVAHFNRLVLSSTATRAELSVSRHDFHPAFHILKSREDAWPDDVRQPTIILSCIPADPIDGGPAAQFTLPAQWMKSPTGGVVMEIAYKTLNTPLMQQVRDEGSRLWAYMDGLDFLPEQAFAQFELFTGKRAPRRIMREEVLRAWRDEQGNPDAEMVQRRLKAIDDQEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJM3</accession>
    <name>A0A9W8YJM3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1996 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000317</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJM3"/>
    <dbReference type="OrthoDB" id="74764at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018535">
        <property type="entry name" value="DUF1996"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43662">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43662:SF7">
        <property type="entry name" value="DUF1996 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09362">
        <property type="entry name" value="DUF1996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040808156" description="DUF1996 domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1996" evidence="2">
        <location>
            <begin position="36"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="496" mass="53279" checksum="F1C15B570F55D223" modified="2023-11-08" version="1">MKSITSLSAAAALLSGTADAFWRMPCQGRIALERLDPIVDPNVASSHAHAIHGGSNFGESTTYDNLRESDCTSCQFKDDKSAYWTPSMHFVHEGGKTEIVPQIGGMLAYYLLLGDDLKAFPEGFEMLAGDSRKRNFTGSVPDKPLAEWGPEDTTQVSLMEKSLGFNCLNYAKTPEGSRYRHSMPTKEYIDDNCANGIRAEIMFPSCWNGKDVTTKDHKSHMAYPSEIDGGTCPEGFDTRLPSLFYETIWNTNAFNGKPGQFVWSNGDPTGNGYHGDFMNGWNIDTLQSAVNDCTDPSGRVEDCVHFIDSLNSEDEMQNQCKIQDLPKILSDDNCAGPANGLCGDVPVQYGPGYAAPLTAGAGGGEPTAVPSLSSLVVPTQSYAPARSEVNGISVYDAPTSAAAPATSAAAPSYAAESAAAAPAVTAAAEVKHASKQDADGSIISTTTYTSAGTVYEVAIKEIAVTVTIEDDSYKARHRRHAHLHRRDREHGLLGRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YKH5</accession>
    <name>A0A9W8YKH5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Stress response protein NST1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">NST1</name>
        <name evidence="10" type="ORF">N0V83_000998</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">May act as a negative regulator of salt tolerance.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the NST1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YKH5"/>
    <dbReference type="OrthoDB" id="21629at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051195">
        <property type="entry name" value="Fungal_stress_NST1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025279">
        <property type="entry name" value="NST1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31780:SF10">
        <property type="entry name" value="LD36051P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31780">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NST1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13945">
        <property type="entry name" value="NST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="260"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="356"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="460"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="825"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="276"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="385"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="460"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="630"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="685"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="704"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="727"/>
            <end position="736"/>
        </location>
    </feature>
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        <location>
            <begin position="765"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="788"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="825"/>
            <end position="835"/>
        </location>
    </feature>
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        <location>
            <begin position="866"/>
            <end position="884"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="889"/>
            <end position="900"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU049441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4378165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1136" mass="125391" checksum="B61F9DC22C7731A6" modified="2023-11-08" version="1">MPPNGTAPHEPTASAVNRKKQKRREKEAAKKAAHDPPAPATGKNGVRMSPALASSGAQTRPSAPQYADPELDEPLYDDEHDFYSDEEAEHYEHAYGTNGHYQQQYGHTPMPSGSGKKPRKKKKAPSAPPPLAYAQHVAPRHSPATGHAIMRSNSGSKKDRIWNTSTQEERERIREFWLSLGEDERKSLVKIEKEAVLRKMKEQQKHSCSCTVCGRKRTAIEEELEVLYDAYYEELEQYAHHQQDVGSFLPEANYGYASSAPHARPMLNTHPPGHYDDDDEDEYSGDEEEEDYSGSDISSEGDYSSEERSLPPPEAADFLQFGSSLQVKGGILTVADDLLKNDGKKFIEMMEQLAERRMQREEDAQFNAHPSMYRSHAGHSHEAPPEEDDFDDEGDEEEGYEDDDYEEDEEDEDDTMTEEQRMEEGRRMFQIFAARMFEQRVLQAYREKVAAERQQRLLEELAEEDERKDQKEAKKAKEAQKRKEKKDKQKQLKAEEKARKDAELAAKEAELKAAEEQRLEEQRKKREEQRKKKEAERKAQEEEKQRKEAERLKRQQEERERQQEAERKAREQKAQEKKAKDDAKRKEREEREAKEKEAREKKAQEDKERKERESKAKADKDRVRKEEQTAAVAAHNAVAGARKVSQPPGVALPPQLLKQNSSTGMPSPHVTPAVPKAPTPSRPRQTSQQGGSHGSSPKTPQVAPGTTKSISPSSQLQNPVMPKSILTKPSGSQPPMNAQHGIPHSSMSPLGPPPGMQGPPGFGMGNMPPGLNGFPGQGSMPGMMGPRPGIPFFPPQPGSQNFRPFPPPGMPTPGSLQMGRGFPMDGPPGFPPPGFPGTNHGFGMGLPSHSRQGSGSFERLSSVDSPIAAPQAQPIQRPAPIQRPSSVKPNDEGRRGHGSDVDDLANHLGSKALLDDEDDIPEPVERRSSVQQHGSMRGAPPLGFGFADAPGSLRSEGYGSFGGTNTIWGTPPLGFPTPGNPWANSPTGPGPFHTIPYGIGITRGDVNERGPGENRVIWLRRIVCSACKSIVARHPTQDGYVDANEVIQHIDATRNPVEPAVSMDEVKSACDIMNGTPNNGGGLLEYKEVAGRLSHIKFVDTAGPAPTYGEIGSPVPSHSVLAGGFGKLTGLGPQGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YKI7</accession>
    <name>A0A9W8YKI7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000802</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YKI7"/>
    <dbReference type="OrthoDB" id="3525185at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053178">
        <property type="entry name" value="Osmoadaptation_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38111:SF9">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38111">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="448" mass="49392" checksum="CC0748F87E7B2F1C" modified="2023-11-08" version="1">MSDVVFVPRVLPAKPSMRSVPRTPRQGSIVIDMTERVVKPRGRKKKEDTRNPDASETATPESSTSARSARPKPPKNVLALAIPEQKSSSTNEQGYPEDVERAITAVRYQYQPPTFHQPSRALPDAFDAAFISHYVELNKSKRTYAPEIQWLVHLPRIHSNANRPAVRLSLRALSMAFYGKLHQDRDILVDSWRWYSVSLNAQRQSISRLSRKRNGIPDEEEVLVPLILALYEVNVGATTSGSMAHSAAAAEIMQMRGPKIQGTLRVFFETPVPAHVDNGPARQLTSKLLADLDRWATTYPNLTGNARGDAPVTAASGTGLSVTNEDLKDQGLPDTFIALIRSNYLAMRLVLNMIMHKMETQSTTPPATPDSAAPQYLTVAIQSAQAILKAAEEVEKRKTPGFDLLRSISPVLAVICTAPTRELGNSASEMLQRWGTRIGGLASVFTRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHG6</accession>
    <name>A0A9W9CHG6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2140489at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36986">
        <property type="entry name" value="UPF0643 PROTEIN PB2B2.08"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36986:SF1">
        <property type="entry name" value="UPF0643 PROTEIN PB2B2.08"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="306" mass="34427" checksum="F6BF410341685697" modified="2023-11-08" version="1">MAPDTAITLVRHDNTSLHIPNDSSKTPNLTISIAELPPTPPEYINHQSPSDHALVLNPAFITQEFDLLRLEAAAYGQLPTTGQVIPNNTPRATSEGTTEGGTTRPELKNSIEFRENVSVSPAFSTHLISSPYNNPGHYLDLSSLPTPSLLFAKALTALKPTRSDYAAAEYTAALNFPAVVAELRSQSEKSGFTWRETSFYVVVFRSRLKEGIDNDWLYKLDYESHREACESGGLLKYWFGKADEERRNLATCFWHSREDAYKGGLGPWHKKARAAGRELYERIVFSTHRFTILDGAEEFRFEDWKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHI8</accession>
    <name>A0A9W9CHI8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009415</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHI8"/>
    <dbReference type="OrthoDB" id="9984693at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033308">
        <property type="entry name" value="PGAP5/Cdc1/Ted1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315">
        <property type="entry name" value="METALLO PHOSPHOESTERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315:SF1">
        <property type="entry name" value="PROTEIN TED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="641"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="190"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="529"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="673"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="190"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="256"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="673"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4363962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="700" mass="77797" checksum="CB5CC791AF4471B9" modified="2023-11-08" version="1">MQLSRLLFRLALLCAPVALISTAWLYLYPTLHRGCAFPTPPATHADVIAPFRLLALGDPQLEGDSSLPNPNARIFPSIEFLIPHLRNATTLDQRRSIVLGATKGVVRDAGRWLEGKRKAIDLWGNDWYLAHIVRSLRWRTEPTHVSVLGDLLGSQWVTDGEFERRAGRFWNIVMRGLEKVPGSIFGVEEAGEQPEPEEATLQEEAAQEDTARENTTEEEAAQEETIQEKTAEEETTQEGTAQEESIQEGTAQEEADDRKEALEGEHSKPPRKPLWGGTTEVLGADKGWETRVINIAGNHDIGYAGDIDQSRIERFEKAFGSVNWDIWFTLPDRNALDTTTGTSASTEPPPTLRLVILNSMNIDTPAWSSDLQTETYSFMNHIITSSRPVTDKTHATILLTHIPLEKESGVCIDSPYFDFFEGGHGVKEQNMLSDHASKIVLEGIFGLSGNKNAAAGGFGRRGIVINGHDHAGCDVLHYIRQAGVEDACQVDDIKREEAYWPVVADTLNNSMSIASADVDEINFEAVSLNETEDASTIGTEADKTSSSPETDTEPIPEPPKPDTSPKWKARRFPPLQYDINEANECTTIRRAPHIREITLRSMMGEFSGYAGFLSAWFDPSLGEKGEWVFEFSSCGLGVQHWWWGIHIMDLGLVVLFIGAGVLSVVEGYEHRNKDQERKEGARKKNEKIRIDQGGKSIKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHQ2</accession>
    <name>A0A9W9CHQ2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RNA polymerase II subunit B1 CTD phosphatase RPAP2 homolog</fullName>
            <ecNumber evidence="12">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">N0V83_010039</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Putative RNA polymerase II subunit B1 C-terminal domain (CTD) phosphatase involved in RNA polymerase II transcription regulation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 12">Belongs to the RPAP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="12"/>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHQ2"/>
    <dbReference type="OrthoDB" id="2590500at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043175">
        <property type="term" value="F:RNA polymerase core enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008420">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039693">
        <property type="entry name" value="Rtr1/RPAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007308">
        <property type="entry name" value="Rtr1/RPAP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038534">
        <property type="entry name" value="Rtr1/RPAP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14732">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT B1 CTD PHOSPHATASE RPAP2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14732:SF0">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT B1 CTD PHOSPHATASE RPAP2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04181">
        <property type="entry name" value="RPAP2_Rtr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51479">
        <property type="entry name" value="ZF_RTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RTR1-type" evidence="14">
        <location>
            <begin position="79"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="240"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="281"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4362922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="299" mass="33474" checksum="0B4653285117F2E0" modified="2023-11-08" version="1">MPPKSILKKTVVSTDAPGRTKPVNPRHLDVALHHANIIEERKRVEAEVLQSIMTLMDFPHSPNADAKRPAATDAHQFREAVVPFQPADYDALIEERNIADKCGYALCPRPKRKARSSAKKHFVDTDHGVEIVDKKVLEVWCSDDCAKRALYVKVQLNEEPAWARQGGYGDSIELMVDNTEEHSKALPLRQKEDTTSPRNEEEDDMAAAWAARDDAVADLALERGEKPGQLSKANKDLIQDHITERVPSNAPPQPPSLPSQSSEHTMAIEGHVPRSDRKDQEEDDDDEDDAQDWDKHLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHU5</accession>
    <name>A0A9W9CHU5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010236</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHU5"/>
    <dbReference type="OrthoDB" id="3946750at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="61"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="166"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="257"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="321"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="370"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="843"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="925"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1030"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="772"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="61"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="108"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="176"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="373"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1080"/>
            <end position="1096"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1151" mass="128177" checksum="7B60C568637C76F2" modified="2023-11-08" version="1">MSSLEWMQLQHYKQWRKRLIEDPYNTMFGASNDMLNGKGLKDWEWISKTFPRWMLREMDEPVENDKDKNKDSYASEFGKKASKDESSAAQTRDNSTCAKSSYSNVANSRHEWDAGSKRRESYFPQPSFRATRLDRDDFTGIISPSDLRRPREQPHVKVVGKAAEVRSESTSDMHHTNSFQNTPVSSASHTPGPAKKIEPVGGQLSELSTSKETSSKEAIARETSFIEQFLADTPPNQSYNANNQIVDDKTWRQTALQRRSSRELISKSAMKPSPIADLSDATTKTTTSREPEAIELPPPQATEHTPEVTTQYKDWLVEQEPPASVTANRTEESTGTGTSRSPLNILDQIPKDDIDFLTAADIRASMGVRRSKIPSDEERRAKRQKLEETFKKTHETSEDINPMIESKIINDQLIRRLERELQQAPDSQGFIKAQTEQHYTEPATVQENVIESSIERMKRWLEQGGAMFSSHFWQDPTEEADATKTKLFFDKIVGRIRKGRIAMRQVVEDLEMEIPATKPLLKRLKDDEEMLDMAIRALRLRVGTGKPHSLTPRKAKAIQALRSKFMDTDRELDAAFTTLNARGKTDAAKSVSLVTRRRISVASKICHKNAHLTRHLIWSLQARLEDPEIDQSILMQYQAVANSLLTLRDTQMALARLVDHAMQIYRVGPTVLENTEFSGQKPSVVDQSPVLENGELERSSSISEAEKANIRAHVAAEERLANEVEAQKSAMRGLSDDGYARTLKLTPKKTFDERSPLAHSLFRPFGPVLESLGKETAENAEAAKANEDAKKKLDDAKLVAQVRKAYEDTYGPITVGHRQVTSSVEEATEGHERDVKTFEMLKDDPTSSAPTVDHASSEQGIDFPALSTEPPLELTKERSTVAPVAHEEVRPHTSDAVAVAENLNEAEPHLDTALGSAKAAAAVATAAEPHNVAPQQHSEISSDSKHSFSSTADLPTHYTILIHDAQTDDLSITTSTTGPPRDISPALALHEALSTLDTPAKFIPYISEGLEIVTAKKDMLVLRDSLDNASSTRSFETIRAPPSTTSTEELETERRTVNPIDGTARLSPTGYVGPEESQEQLEKEFEERREAADRFSRKGGKQQDYSQDQKETKGRRKGGAGSVAKTAIWAAAVCYVVGVFGEIATTSPFQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHV0</accession>
    <name>A0A9W9CHV0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009078</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHV0"/>
    <dbReference type="OrthoDB" id="4034134at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038872">
        <property type="entry name" value="Put_GTT3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41807">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41807:SF1">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="344" mass="38097" checksum="ED0B729B542C2479" modified="2023-11-08" version="1">MSHSWLQRKRKGELLDLAQKAKLPDADSYLKDDLVELLEEHLNSNETTYAKHPEFRDYYGRSGSPVKRERSSPAEALTVTKSRRRTLVKAPSEEPTPEKSSLITRTPRAVSRRVSQVLSEVDIPASPAQLAEVADQSFQVAKTKATELWDRTQIDRLKEVVRENASSVAAIQTLILLIEAIGLQWKTLDTTAAASLPDGVSKHTGQNMLMVPNFTALLSSQWWAPATLWSLTSWVLPLVFSYFFNLTLSANTSRKSSSRQYPADPFTFNIVKAILVYSAYRVVEISAGSIGDSAIAHDVNYSWGPFSADTVEVVRENVPGKYPGLLISSAVGILVSLYDAALKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIF8</accession>
    <name>A0A9W9CIF8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_008947</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIF8"/>
    <dbReference type="OrthoDB" id="5788137at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF43">
        <property type="entry name" value="STEROID 5-ALPHA-REDUCTASE DET2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="7">
        <location>
            <begin position="34"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4365326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="201" mass="23421" checksum="44EAD42445CAF3F3" modified="2023-11-08" version="1">MAGCTYQEQVIHYIYRAIISPLFLNPSMSPMGPLVWLMAFAFQIFNAISIGGYLAGYGPTTQYEWAARSEWMFVGLVIWCWGLMANMFHDDDLREIRRTADRKQRKEAAEQGKPVESVDKIYMIPKNGLFKYVLHAHYLCEWIEWAGWWMIGGWKCVPARTFLLNEISTMLPRALQGKRWYEKKFGQERLEGRKAVIPGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIT1</accession>
    <name>A0A9W9CIT1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">T6SS Phospholipase effector Tle1-like catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIT1"/>
    <dbReference type="OrthoDB" id="3162439at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018712">
        <property type="entry name" value="Tle1-like_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33840:SF2">
        <property type="entry name" value="TLE1 PHOSPHOLIPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09994">
        <property type="entry name" value="T6SS_Tle1-like_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T6SS Phospholipase effector Tle1-like catalytic" evidence="2">
        <location>
            <begin position="15"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="543"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="564" mass="63306" checksum="6DBE32D8F625A652" modified="2023-11-08" version="1">MPYVESPPASPNKVRKLVLCFDGTGNTFQGNTMDTNVVKIYDKLDRDNENQYHYYQPGIGTYDISGGSLNKGTIGSFKSKISKAVDSGIATSFDAHVMAGYKFLMNYYEPGAKIYMFGFSRGAFTARFLSRMINTVGLLSKGNDEMIPFAYSLYQAYEQANDEAQVDKDSHGEDDDETSALLNDSENAKRKLNNFTSTFCRRDRISQPGEEVSYARIKVYFLGIFDCVSSVAVLETPFGKTPKPVSVLGTAEHVRHAVAVDEFRVKFKAALLNQDLMHEKYTKAEDVKEVWFPGNHGDVGGGWLAVKQETKPVQRGWWQNFTANVKRIIIGSKGSTATENVSQDPYQMSDIPLSWMIHELELIGSKEEAFALQWSKRLEGFKRNFNEQEAHAASRHNTMSFGRGSSLLKVLMWKFMDYVPLFPRYELQGSGQWEMRTWPPNLASARDIPKSALLHASLLHRLTAQRPRTYTPRNNHGSGGPCLVADKFIKINVKEPSFGHKADHTLYALDQKTVEKYEEDAEKPHDHSGRITRSSVVNGVSSFKSSSSFKSSSVGVSVNEIHTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIV2</accession>
    <name>A0A9W9CIV2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ureidoglycolate hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_008507</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(S)-ureidoglycolate = urea + glyoxylate</text>
            <dbReference type="Rhea" id="RHEA:11304"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57296"/>
            <dbReference type="EC" id="4.3.2.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIV2"/>
    <dbReference type="OrthoDB" id="10266039at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004848">
        <property type="term" value="F:ureidoglycolate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050385">
        <property type="term" value="F:ureidoglycolate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000256">
        <property type="term" value="P:allantoin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20298">
        <property type="entry name" value="cupin_UAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.480">
        <property type="entry name" value="Ureidoglycolate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047233">
        <property type="entry name" value="UAH_cupin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007247">
        <property type="entry name" value="Ureidogly_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024060">
        <property type="entry name" value="Ureidoglycolate_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21221">
        <property type="entry name" value="UREIDOGLYCOLATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21221:SF1">
        <property type="entry name" value="UREIDOGLYCOLATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04115">
        <property type="entry name" value="Ureidogly_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4365885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="34981" checksum="86DA8B690495C162" modified="2023-11-08" version="1">MPTSMPAPSIRIPIELLTPPAFHQFGTVIENPATSPSAKLPIPHRVPPPDSVTANQGFATKYLDVTHMSNLYTLAPSKKPAKAVMNMFVCKPRELRPHEASMSLPSSWGDLDIDDDEDENGNYERQLLDIGILERHPFTTQTFIPMGLAQQDKSTQYLVIVAPTLPASASRRYSNRPPPYPTPKIERKKSFRDIFARARPSPFTNEATPPPSLFSRLHPSARPKGPGIPDLKNLRAFVATGSQAVTYGAGTWHAPMIVIGDRPIDFVVVQFANEVGIEDCQEIILKPDSTAPEGVVVMVDTDSSGQVQVKTGVASVKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKU9</accession>
    <name>A0A9W9CKU9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_007113</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKU9"/>
    <dbReference type="OrthoDB" id="5296287at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140115">
        <property type="term" value="P:export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042908">
        <property type="term" value="P:xenobiotic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000460">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF33">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="340"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="445"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="513"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="105"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4367530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="547" mass="60472" checksum="A5C439BB10AB2A72" modified="2023-11-08" version="1">MNVEKERAVPSSRSSETQIPDLEKGEARDPNEEEEVINEKELEGTRDDDMGVDLEKHLSRKSTKKDQLPQEKYPVSDLDNGLVGWDSQDDPINPRNFPNGRKWTLLGLVAAITFLSPLASSIFAPGIPFVNADFHNTSQLLGSFAVSVYILGFSFGPLFLSPLSEIYGRRIILNYSNVFFCAFTLGCALAPNLGGLIGMRFLAGVGGSACLTIGTGVIADMFVPAERGKAVAIYSMGVLFGPIVGPICGGFIAQRAGWRWDLWVVFIAGCLLTGALFVFNRETYHSVLLKHKTERLRKELERPELQNIMTYNKDASALSPRDILKRGIIRPLRMLTTSPIVLFCSLYMSFLFGLLFLLFTTLTTVFIETYGWSAELTGLAYLGVGIGNFMGIITVAKTSDATIIRLAKKNNGVYEPEMRLPFCIFFGVLIPISFFIYGWTTYYKVHWIVPIISLVPFGLGLMGIFAPLQTYMIDCFPQYAASAIAGMTALRCLFGALLPLAGPSMYETMGLGWGNSMLGFLAVAFIPVPALLFKYGKSIRERWPIKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL01</accession>
    <name>A0A9W9CL01_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U1-C C2H2-type zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_007145</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL01"/>
    <dbReference type="OrthoDB" id="191651at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013085">
        <property type="entry name" value="U1-CZ_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040023">
        <property type="entry name" value="WBP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13173">
        <property type="entry name" value="WW DOMAIN BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13173:SF10">
        <property type="entry name" value="WW DOMAIN-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06220">
        <property type="entry name" value="zf-U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="U1-C C2H2-type zinc finger" evidence="5">
        <location>
            <begin position="9"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="29"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="73"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="183"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="89"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4367561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="277" mass="30724" checksum="C3B64268D6CC2455" modified="2023-11-08" version="1">MAEYWKSTPKYWCKFCSTYVKDTKFERAQHEATGRHQGSIQRSLRGLHREQENEQRQKARAQAEVARLNGLVPSASTPSVATGVGGKPTYEKRPEKKATIDDRKRQWEQLAAMGVALPDEARGDMAIAGDWKTVSHEVVGEVNEAGEFKAAPLNTGVRKRKIDEEEEEREAAGEMITKKKGWGHKYKTFPGSKGGDDDFESLLSKKPAVQAEDPGVKKEEDDDVKAEAPDESEAKTLQDIPTVEEAEAKATDTTVKQGEDAPAAPTVVFKKRKKIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL67</accession>
    <name>A0A9W9CL67_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">RHO4 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rho4</name>
        <name evidence="10" type="ORF">N0V83_006503</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL67"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032506">
        <property type="term" value="P:cytokinetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000678">
        <property type="entry name" value="Rho GTPase Rho4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="243"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="268"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4368147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="279" mass="30858" checksum="D5632974F6FEC568" modified="2023-11-08" version="1">MASHTAPSQNYDYLRRKQSTKAPVSSITPVRKPVRTPSERNSNGTVSSYGTTNTRDTTITEPPSYSKKLVVVGDGGCGKTCLLISYSSGNFPEKYVPTVFENYITHTPHPPTGKMVELALWDTAGQEEYDRLRPLSYPETDIIFVCFAIDCPNSLENVMDKWYPEVLHFCPTTPLMLLGLKSDLRNKKNCIELLKTQGLTPVTPEQGRVVAKKMGAMYMECSSKEQDGVEEIFDMAVTMAVGDEYKTPQQPNRPTSSRGGGPAFPTAGKKKKRSGCKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLJ8</accession>
    <name>A0A9W9CLJ8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006760</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLJ8"/>
    <dbReference type="OrthoDB" id="5427780at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="131"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="314"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="387" mass="40869" checksum="851D3CC6BE374720" modified="2023-11-08" version="1">MADDPNNPPRRRVSKAFLFGLSNKPFKDDPPPRPAQRSAFNQMVTTPGGLIEWVASDGRKGKLTGAGRQRLTEASLAKVPSEKRTVKEALSVKDKEKKAATEKSASMVGDLFGGMDLNEGAAGRDTKKKQNDGDKANTWTAEEDKKMMDWKNENPSAPWAAFAEEVNKSVDECKAHFKDIKPADWKPTGAKGKGGAGAGVNQQKSGKKDKKQNQGNNNSGKKKEEASGGEPENQWGGMDVGGGWGNDTGNNANADVGGYQGWGTNDAVWGNTNPWGIDNTINDNNGVANANWGNDNAASGGAPGAVADTWGASATTGGVPNNTNTNPVPAWNTTAAAKPASKPPSKAPAPPPHAPTTARRPLPPSPRMRRVVFPSRSTPTRPSREAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLK5</accession>
    <name>A0A9W9CLK5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLK5"/>
    <dbReference type="OrthoDB" id="419598at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052718">
        <property type="entry name" value="NmrA-type_oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47129:SF1">
        <property type="entry name" value="NMRA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47129">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="1">
        <location>
            <begin position="3"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="312" mass="35138" checksum="A94C3DFE664ED779" modified="2023-11-08" version="1">MLALTSATGKLGSAVLNAILDNKLIDPKELVVCTSSSPTSEKFSNLRDQNISVRYANFDEPDSLKSAYTGCNSLFLVSTPRIAMDYNDAPLWSGREAHHRAAIDAAISVGVKHIYYTSLAFANPSKASVMRAHIRTEKYLKELEEQGKVGVTIIREGLYNESWPLYFGYYFGLKKENRSEVLVAGDGKISWTSIPDMAFGTAKMLAAPSEEWAGMTFYLSQRRTWTLENIARIVSKIKGEDVKLQVVSRSEYENFYVQEKEMDRPSVEWWSSSYDALRDGECAIEDPTLERLLKEAGRSPKPLEETIEDMLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLY1</accession>
    <name>A0A9W9CLY1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005168</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLY1"/>
    <dbReference type="OrthoDB" id="3766782at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040814482" evidence="2">
        <location>
            <begin position="21"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="340"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="349" mass="38514" checksum="DA63314C865127BB" modified="2023-11-08" version="1">MRINVILAATILAATASGAALPVATEEAAPATEAAEPEVPEVPEGFPEDFPFGPYGPVYQKRDAQPGWSWTPWRPYGTPVGKRDAKAEPGWNWTPYRPYGTPIGKRDAEANPEADPEAGWNWTPYRPYGTPIGKRDAEAEAGWNWTPWRPYGTPVGKRDAEADAEAGWGWTPWRPYGTPIGKRDAEANPEADPEAGWNWTPYRPYGTPIGKRDAEADAEAGWGGTPWRPYGTPIGKRDAEADAEAGWGWTPWSPYGTPIGKRDAEADAEPEAGWSWTPYRPYGTPIGKRDAEAEAQWNWTPYRPYGTPIGKRDAEAEAQWSWTPWRPYGTPIGRREAEAAPEAQPAAEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMW1</accession>
    <name>A0A9W9CMW1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004317</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMW1"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="93"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="336"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="569"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="627"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="493"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="198"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="585"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="648" mass="71470" checksum="CFBBFBBA4A38DFA5" modified="2023-11-08" version="1">MARIKVTAGRPKPTRRPRDDEESSDEPAKSQKPGRISSPGERPKVTAGRPKPTRRLRDDEKSTKEPMKPQKPARVLNTGERVKVLARKPGYVAPSRRIAHQKPARVFNPGDRPKVLARKPGNTAPRRGKLDLYDLETEDDMSSSDTESGLGASLEAEDAMSDTSSSSSGDDTFESLIEPEVNPLQRASRIGVSPEPKDENDTEVSESDEPESEDLDNEESENEHADSEDSASELAPEPTLRRTTAKVETSSRDTTSSLPSLDSDDEMFEPPPKRRRIAVSPGDESEVHAVSNDQLHSTDMEDDQHADMGSEHTPSDSVMNDIESDFDRQLYLLDNDLSDTKSSPSRAGSDVAPKSVASNGLDTPARLNAITTLAEYANGNNVANLAKFIDENADRTLGNYTNGHAGAGSDRKVHSPWNHVSHRKTYVSFGPREVHHSTQLAAVHLFNRPINDEQAMVNTLRMPRVGPILAANIRAETSQDSRGVYIPVRDALVAEKKLALEAAHDDLKAAEVQLRSLEVKHRMSTLRLATARAQRKPKVRVRDLKKMQAQVDEAAQDVNDAKLNVDDERKAVDEAKEAVQGLGGEIEEEEEEEEEEEEEEEEEEDNGVVANHTALIADINAKIQEAEEVEEQAPPAAVQKVKKRKRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN52</accession>
    <name>A0A9W9CN52_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tricarboxylate transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_004020</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN52"/>
    <dbReference type="OrthoDB" id="44467at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071913">
        <property type="term" value="F:citrate secondary active transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006843">
        <property type="term" value="P:mitochondrial citrate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049563">
        <property type="entry name" value="TXTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788">
        <property type="entry name" value="SUCCINATE/FUMARATE MITOCHONDRIAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788:SF3">
        <property type="entry name" value="TRICARBOXYLATE TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="7"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="104"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="200"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4372246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="32197" checksum="6D4501613F0772F4" modified="2023-11-08" version="1">MLKDNNTPAVVSLAAGGVAGGIEGFLSYPLEFAKTRVQLRAEKGVPTPRNPFRVVTQVYQNEGIRALYKGCGALVVGSIAKDGVRFLFFDQIKESFADPETGTLSPLRNLLAGMTAGVIASITAVTPTERIKTALIDDARTEKRFRGGFHATATIWKEHGILGLYRGFAGTTLKQASATAFRMGTYNILKDYEKKRNIEQNTAVNFANGSVAGVVTTLATQPFDVIKTRSQSARGTTTAEAVKSILLDYGIRGFWKGTTMRLGRTVFSGGILFTSYEAAVKIIMPLYSGVKHTPGVEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN97</accession>
    <name>A0A9W9CN97_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_004784</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN97"/>
    <dbReference type="OrthoDB" id="72726at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4371565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="352" mass="40287" checksum="FC85F83968CB58E6" modified="2023-11-08" version="1">MLAPSQINRWRESSSDYKMDAPNLLTIPVELRELIYGFLFSGYNIRHGFKSSHIPDDKSESSNRIALLLTCRQVFAEAWRHLPLNCTLHFRGTENLLDTLLSVDQSVITRIRHIRVRAFPFPLYASGRAEYYPTYYAANALTLLPGLCLDTLVVEDCWHGFGMGDGWRDVTTYFDIETLLKSAAWKELTYITPCTDFIASGYDHRRKRLAQPETWDEMIKESDGAESGATVKMFIVLYKQEKATGDERTEDGRIMQPWAAKPGHEVIENWRVAGPEQDLKGEVRIVARRGKMARPVQLGLSEKRTWKELKGKEGGFVPEDWKPYHNDMADAMGWLYGGWGRRMQLANSALNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNC9</accession>
    <name>A0A9W9CNC9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_004290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNC9"/>
    <dbReference type="OrthoDB" id="202289at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053143">
        <property type="entry name" value="Arylsulfate_ST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039535">
        <property type="entry name" value="ASST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35340:SF5">
        <property type="entry name" value="ASST-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35340">
        <property type="entry name" value="PQQ ENZYME REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14269">
        <property type="entry name" value="Arylsulfotran_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4372516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="373" mass="42073" checksum="6F7668FE46DEFC29" modified="2023-11-08" version="1">MEQSTLARRGLGLRGIDPHKVSPGYTLFAHLTSPGTVNLVDNHGQVVHQWNLPYRPGRHAQLLKNGNLAYNGVHPDAPRLFPLWQKYRGGVMMEVDPQGNVVKEHRDPLAHHDQHHLDNGELLYTTVEGLEPEKGACVLGGIPETEAPDGKVYADCIKHVSESGELLWSWKAIDHLDPKKFKLQPHYAREHWPLINSVYPLADGNILASLRSVSAVVIISRQTGEIIWHLDSTVVAQQHCASELEDGSILIFDNGTFRHHESATYSRVIQVSREDKSIVWEYRDPHPMTFFTPFMGGAQRLRGGNTLVTEAAFGRVFEVTKDGEIVWEYVNPDFADYTGLDAKEIEGYFAYPANALFRAYKYSPEEVPWMHPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNW1</accession>
    <name>A0A9W9CNW1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ethanolaminephosphotransferase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_004232</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNW1"/>
    <dbReference type="OrthoDB" id="196717at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000029">
        <property type="entry name" value="CDP-alcohol phosphatidyltransferase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014472">
        <property type="entry name" value="CHOPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414:SF77">
        <property type="entry name" value="CDP-ALCOHOL PHOSPHATIDYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414">
        <property type="entry name" value="ETHANOLAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015665">
        <property type="entry name" value="CHOPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="295"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4372458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="424" mass="47545" checksum="CAB95CD8FDE2DF2D" modified="2023-11-08" version="1">MPPSKEAAKHECVSEDALQHLRTYKYSSVDKSFISRYILKHYWNGFVELLPLWIAPNLVTLLGFFFILGNVVLLELYVPDLVGPAPSWVYYSFAFGMWMYSTMDNVDGKQARRTGTSSPLGELFDHGIDSLNCTLASLLETAAVGYGSTKIGAFTALIPVLPMFLSTWETYHTHTLYLGYFNGPTEGLILACLFICMSGYFGPEIWSTPLATYFPSYKAEIGDVTLKELWIPIILFTFFVAHLPACIVNVARARKARNQPLLPTLYEWTPLVIFVVCSMAWLGSPYSKLLEDNHLVLYCLTMSLVFGRMTTKIILAHLTHQPFPYWTIMLAPMIGGALLVNHPYFTIPGTTFGPLSASFELWYLRAYFVFAAVVYGRWAHLVITSICDYLGINCLTIPKKTAEKNARSNGAANGIHPEKGGRQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPW8</accession>
    <name>A0A9W9CPW8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription initiation factor TFIID subunit 12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_002407</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPW8"/>
    <dbReference type="OrthoDB" id="2193432at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07981">
        <property type="entry name" value="HFD_TAF12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000037">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037794">
        <property type="entry name" value="TAF12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003228">
        <property type="entry name" value="TFIID_TAF12_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12264">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12264:SF21">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03847">
        <property type="entry name" value="TFIID_20kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 12" evidence="7">
        <location>
            <begin position="532"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="73"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="201"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="251"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="169"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="251"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="264"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="371"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="396"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="408"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="441"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4375321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="642" mass="71460" checksum="78F7FDCF59813550" modified="2023-11-08" version="1">MSDSQPQQAGAGSGQQQQKLLRADDILKLQSLPDDEKQKYRLIMQNFWNMIQQHPQGSPEHATAFQKLSEWSQKFIARERTHRGKMKAQQEMKAQQQQQQANQGPSSQGNQPAQQMPVKQEAPPVANTGAQGAAASSSQGQQPSSQGQPRPQNQVDPAILKHVHEFPIQLPPNAPPPGTPEYDVKLKEYRNGYLNMLAKQASHAENRRRLTNQIQERQNAGQEVPQEILTMKARVEKEHAVMKDQLDKFRKLQKQWKDEREAKQAQQQPQGQSQGQQPSTASPQQQQQQQQPQRQPSQPNMPTQPQIKEEPQIKVEGGQVPQPQPAAQFNMQGNQQQTPQQQNPQIPPSLPQQQQQVRPPPAPHSQSMPMNQGQQFAQPGQQQPNFQQQQQQQQQRPQINPMQANAMQHQQSNSPHPQSATSNAPAGPPVPLSHQAAVSAANRSYTDPQRTNTPMQQGGQGNFGSREREQLNNPKMPIPRHLNVSSPSGVHMGQARPTMQGPTNGAPGPMGQPVIPRPPPFQLEGEGDRVLSKRKLDELVRQVTGGSEEALTPEVEEAVLQLADDFVDNVISNACKLSKLRESPQLDIRDIQVILERNYNIRIPGYASDEVRTVRKIVPAPGWAAKMNAVSAAKVMGGKTDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ09</accession>
    <name>A0A9W9CQ09_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_001049</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ09"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072:SF3">
        <property type="entry name" value="AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072">
        <property type="entry name" value="AMIDASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="5">
        <location>
            <begin position="79"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="3">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="231"/>
            <end position="234"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4375772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="520" mass="57204" checksum="47906D5AA1D0B718" modified="2023-11-08" version="1">MSEGRKDYQTISIEKRLQQQSKIPKEWILPKSYHGATNFMQVPSTCGILSDVECNITSNFDATALLEKLKSGEWSAEQVTTAFCKRAAIAHQLTNCLTEVFFDKAIERALELDRERQANPGRPLRPFHGLPISLKDSFQVSGYDTSTGLACFVNEPAEEDSGTAAMLLDLGAVLYCKTNLPQTIMTGDCDNNVFGRTLNPRNTALTVGGSTGGEGALLALRGSVLGVGTDIGGSIRVPSVCNGIYGFRPSVGIVPHGGVRDLTTPGTDGVRSTAGLMATSLRDINLFLKTTMQAKTWHYDSTVISVPWTNLAPKKRIRIGLVLDDEIFTPSPLVRRGLRKTAALLREIDSVEIISLTLPNVKEHYGDLIKYFALLGSENYLELFARTGEPEVPAVKAIGLLSITGTDLRGFFDLNVRRQAAARDYLRIFLDNNIDAILMPPAPHTAVPLDQWTTASYTGLWNYLDYPAVIIPWTQFASLTWLMMLAMLNTAPRMPIPDLKITKTLPYAFSWLATAMQMKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ49</accession>
    <name>A0A9W9CQ49_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Domain of unknown function at the cortex 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003083</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ49"/>
    <dbReference type="OrthoDB" id="2119945at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013897">
        <property type="entry name" value="Duc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34826">
        <property type="entry name" value="UPF0590 PROTEIN C409.17C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34826:SF2">
        <property type="entry name" value="UPF0590 PROTEIN C409.17C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08588">
        <property type="entry name" value="Duc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Domain of unknown function at the cortex 1" evidence="2">
        <location>
            <begin position="9"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="324" mass="36288" checksum="155D556B83C38159" modified="2023-11-08" version="1">MAQHEQYMLRITAGATYDSTKHKDMLVNSEKPVTISSDLIDAKIHMRIKDYRGLPKGSPSTSPYFSTPPHPYDRYSISFSFTPKSSIPGDKLVFGNDFDHPIRDRLPPLFDKAFGVVKWWIDPGLDGDVQADEPYLYGALLSSINVLDIGEKGDGKTSSKEILEGHAKTEGDVEKSGSEGEAVVYQEGAHGSGTQIRKEKGIPDKAAARQKFFLTEQNRKDFTFEAGREYRCDFFNPYLDFNEFALKIGYGLPNISIIGHWDGQPLRYVLKNRETNKELFVVLIQLLPTEKAKKEGAEDPEEKFEEVHGGDGGKAQDGDDDELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQC3</accession>
    <name>A0A9W9CQC3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADH:ubiquinone reductase (non-electrogenic)</fullName>
            <ecNumber evidence="2">1.6.5.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">NDE1_1</name>
        <name evidence="13" type="ORF">N0V83_001383</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a ubiquinone + NADH + H(+) = a ubiquinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:23152"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.9" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQC3"/>
    <dbReference type="OrthoDB" id="3244603at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) (non-electrogenic) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054585">
        <property type="entry name" value="NDH2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF47">
        <property type="entry name" value="EXTERNAL NADH-UBIQUINONE OXIDOREDUCTASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22366">
        <property type="entry name" value="NDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="11">
        <location>
            <begin position="112"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="External alternative NADH-ubiquinone oxidoreductase-like C-terminal" evidence="12">
        <location>
            <begin position="509"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4376102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="577" mass="64707" checksum="697B8A6C78F10346" modified="2023-11-08" version="1">MAPNMRPLAAANLLSQVQLAGRRTFTTQRPSLIASRAQWTPRPVQSQMVLRQAVRQQSTAPPPKPKRRFRLLRWTWRLTYLSALAGLGYVGYGIYEMRNPADQPPPDPSKKTLVVLGTGWGSVSLLKKLDTENYNVIVVSPRNYFLFTPLLPSCTVGTIEHRSIMEPIRNFLRHKKASVKYYEAEATKIDYEKKVVYINDDSPIKGDITQTEVPFDMLVVGVGAENATFGIPGVKENGLFLKEVGDAQRIRNRIMDCCETATFKDQSEDEKKRLLHMVVVGGGPTGVEFAGELQDFFHSDLKKWLPEIQDNFHVTLVEALPNVLPMFSKQLIDYTEKTFKEETITIRTKTMVKNVTEKFIEAESAGPDGKKQLEKIPYGLLVWATGNALRPLVKDLINQIPAQKDSRRGLAVNEYLVVKGTENVWAVGDCAVANYAPTAQVAAQEGAFLARMFNQMAKTQEIEADLADLSVAQEKAPGKEARDQVFSEIKALQQKLRRIKQIGPFEYSHQGSLAYIGSEKAVADISWFSGNIASGGTVTYIFWRSAYLSMCFSTRNRILVLMDWVKAKAFGRDVSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQD9</accession>
    <name>A0A9W9CQD9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Carboxypeptidase</fullName>
            <ecNumber evidence="7">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_001876</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQD9"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000118">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF479">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041020880" description="Carboxypeptidase" evidence="7">
        <location>
            <begin position="19"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4374799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="555" mass="61354" checksum="3C6BD1DEE88941DC" modified="2023-11-08" version="1">MKLLTLFGTLLCASSALAGSSPHKRNVFNKVKPVLEKRVANEPFKHPEIRKRASGFLTDKSKPFAVNGTGIPEVPFDIGESYAGLLPISDSPDETRKLFFWFFPSTLESTPEEVVIWLNGGPGCSSLSGFLTENGPFTWEAGTLAPVQNPYSWNNLTNVLWVEQPVGVGYSQGTPNITNEVELGDEFRGFYKSFIDLFELYNWKTYITGESYAGFYVPYIADSFIKADDAKYWNLAGISINDPIIGDDTIQQQVVILPFVEYWQNLLYLNDTFMETIRKHDAECGYSGYLEKYFTFPPPQGPFPLLPDPFSTDKYICDQFDNFYAAILEVNPCFNIYHITETCPHPYSQLGIVNTGDYSPPGAEIYFNRTDVKQALHAPLDSNWLQCTNINVFGNGNDSSDASDTSPGPANDGVLQNVIEYTNNTIIGSGDLDMLLSTNGTLLAIQNMTWNGAQGLSDYPSTPLYAPYHPEYNGGALAGAGIAGVWTSERGLTFYTAHLAGHELPGYTPGVALRMLEILLGHVKDFSSTDNFVTQSGNFTGNSTIYKRGAMEMFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQL1</accession>
    <name>A0A9W9CQL1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQL1"/>
    <dbReference type="OrthoDB" id="10252171at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4376357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="223" mass="25757" checksum="416BCEC2F5F46228" modified="2023-11-08" version="1">MILRPARVFKVARMLSTFVGQSGREYKLHKVLQRHPKKPEVSVCLALCNYKPFVLKPVSQSIFDHLQEFRTEFGSHPRLRNHIDQNEKDSIVFYEYFKTDLLSLCLYVITGVQSFNLNFDEIDTEPEVAVLFKLLATFGPLPDDALVTHVKDMKAGQLLTDFWQVIWEREVRGSFVEWEEDVFPNLDDGAKRLILRMTKLDPARRAPMSDIGTDAHWGVDEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQU5</accession>
    <name>A0A9W9CQU5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQU5"/>
    <dbReference type="OrthoDB" id="368909at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06168">
        <property type="entry name" value="LSMD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034110">
        <property type="entry name" value="LSMD1_Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050914">
        <property type="entry name" value="snRNP_SmB/NAA38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701:SF5">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 38, NATC AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B AND N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="1">
        <location>
            <begin position="35"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="132" mass="14681" checksum="0F9258F3E67A236F" modified="2023-11-08" version="1">MTAANSRRPSRALSWLLPWMQTSTTMEHEQATFWLSQFIGKNLRIHASDGRVFGGQMKCTDKDRNIILALAHEYRAPSAETIRQAVEESGNPSVGVPWNSRYVGLVVVPGQHVTKIEFEESSLPGQKSGVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQW7</accession>
    <name>A0A9W9CQW7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQW7"/>
    <dbReference type="OrthoDB" id="3913028at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="175" mass="19301" checksum="AA037CAFC1D7BBCB" modified="2023-11-08" version="1">MLEESMTFKSVSTSGTASSKASSLFDLDEHGNKSILVYRYRSGAKGLEVKTERALCEELDKDPSLPFMRVTRKPIPRGNSRLDILGEDLMMPEERSKYAEKPTLKASVANVGLPTLAKWKHMILGSYDDLYIVSRSVTPTPESSKNNSPESSQHVSPATTFPSSDANHPLRNPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQZ0</accession>
    <name>A0A9W9CQZ0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_000231</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MSOX/MTOX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQZ0"/>
    <dbReference type="OrthoDB" id="2219495at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051698">
        <property type="term" value="F:saccharopine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008115">
        <property type="term" value="F:sarcosine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045170">
        <property type="entry name" value="MTOX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961:SF26">
        <property type="entry name" value="L-SACCHAROPINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961">
        <property type="entry name" value="PEROXISOMAL SARCOSINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="7">
        <location>
            <begin position="10"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="416"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="423"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="440" mass="48321" checksum="17490C6845F8A8E7" modified="2023-11-08" version="1">MVASRANTTVVVVGGGGTIGSSTALHLVRSGYSPSNITVLDTYPIPSNQSAGNDLNKIMGIRLRNKVDLQLSLEARQMWKEDDLFREYFHNTGRLDCAHGQKGISGLKQAYQALLDANAGLEETNEWLDSEEEILKKMPLLDREQIKGWKAVFSKDGGWLAAAKAINAIGEYLRAQGVKFGFGGAGSFKQPLLAEGICIGVETVDGTRYYADKVVLAAGAWSPALVDLHDQCVSKAWVYAHMQLTPKEASAYKDAPVVYNGDVGFFFEPNEKGIIKVCDEFPGFTRYKMHQPYGASAPKRISVPRSHAKHPTDTIPNASDASIRKAVATFLPQFKNKELFNRAMCWCTDTADAALLICEHPQWRNFVLATGDSGHTFKLLPNIGKHVVELIEGTLAEDLAHAWRWRPGVGDALQSRRSAPAKDLADMPGWKHDEAPRAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRH4</accession>
    <name>A0A9W9CRH4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_001568</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRH4"/>
    <dbReference type="OrthoDB" id="5226580at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF39">
        <property type="entry name" value="TRANSCRIPTION FACTOR PBCR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="25"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="87"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4376285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="740" mass="82633" checksum="D6B6AC06D36CD8E7" modified="2023-11-08" version="1">MSNHLVDSPEFEIPKKSQDQPLNRACEACRVSKVRCLVNPVAGSTQCQRCAKAGKACVFAPPAKRRQRKRTDVRVTELEKEIQQMRSLLKSTNRSSNDISDHESGDEHSDEQAEPGEKGTPQDPQSHGSAASTTSTFANQWPSVPVEKASVPGPSTNCGPRDLLGPSENDIIDRAVITEDQADELLSIWRTELLMGCPGVTIPREWTVMELRARKPALFHAIMAAAAHSKGSALSDKLHEEAVYLYARSAFIKGEKSVQAIQALIVTVTYYSPPKTPGQLQIYQWVNMAASMALELGLASKPRTHEQLPKRAIRSLQKISSPEELLEHCRTVLLLYIISAGFSMRLKRPNILLFNSWMEECFNILGKSKSVDDKRIIAWLKLQRIADEANTAFGFDDASTSFSLSELRMQIILRIFDRRMQDWKNSVPDDVLTLYATIEFHMNMLSMWEFGMDGGRYDVTEYRNRHLTLPALDDDCVQPESLLSRSALQINATTKCIGAAHSILECFSSIPIDKLQKAPNVVYVRAIYALVALMKAEYAVGTDAEMGELLDSQSLKVEYYLDMVIKKASEARGPQKCRIPSHWSFVLEAKLKSWWDEYLEWKKEGRHLKRRRLNEGDKSVPSAGDQTLNFDEVKNTTSTSLQQMQPTTNQGMPTGSRFVLDNNAYSSWSNNEGHLDTTASAHTLGDQATFTSDLGDFSATFQNGDLYLWNDMSVDSFGGWGPQGGLYTGMGYGPLNGQGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRK2</accession>
    <name>A0A9W9CRK2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001643</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRK2"/>
    <dbReference type="OrthoDB" id="2157530at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF82">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26639">
        <property type="entry name" value="Het-6_barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="51"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="595" mass="68322" checksum="DAF6E359125263EF" modified="2023-11-08" version="1">MDDANPATSYPAEDLDLHLRQIRLATILPGRYIDTVACNLSTAYVDDLPEYYSLSYVWGDRKNPRQILLNGVPFDVTRNLQAALRRLRHPTQSRLYWIDMICINQNLGEERTHQVNLMRLIYSRATEVFLWLGDIAETTNQEASPALQNNDSEECLNKAEAVRAVSHIELLATGQHLRYDAQGDEQLRLMAELRALDKLMRSTWWRRIWTVQEAILPPKVMVMVGWLQVPWATLVAAARSVPKHGLECCLGVKAAEWNILDTFNERVAQIDYFRHRYHQGHHPYLSGLLHDFRTRSSTDSRDKIFGLLGLVTEDLKYNELQADYSLPTDAVYRQAFIYLLQQKKSVNLLVRAIELKRTLDLPTWIPDWTAEVDEATANIELNIIYTLHLYNASQSTQSDIVYPNTSNMLGVKGLLFDTVATLPEALSHDPWPTEPRIEEYVKSLYENMGRPSGSYMGRESLARAFRRLFSMDKVVNVTGDSFLRATGRDYGKYPNTCLLYPLRSHNWYLPPFRFFITKKGYIGLGPRNMRVGDSIHIFLGGNLPFVLRQVPEQIGVTEPQETRYEYVGYCYVHGIMDGEALEGVDVDNLGYFNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CS18</accession>
    <name>A0A9W9CS18_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7820 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_000475</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CS18"/>
    <dbReference type="OrthoDB" id="5384459at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056722">
        <property type="entry name" value="DUF7820"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42078">
        <property type="entry name" value="GLUCAN 1, 4-ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42078:SF1">
        <property type="entry name" value="GLUCAN 1, 4-ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25130">
        <property type="entry name" value="DUF7820"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="380"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7820" evidence="3">
        <location>
            <begin position="433"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="647"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="747"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="647"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="658"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="761"/>
            <end position="774"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4377647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="784" mass="85019" checksum="8E5D8A9FDBB59EC4" modified="2023-11-08" version="1">MERRSSDSDEPSRRLSNPNVFDDDYEIDPSEEDFMPSTSDGFRPASTSEAWRNDRQDHDRNESPQRQTSTTKPNDSDLRRMATRNSVAKVRDPPTQDGRPLLNRRPSAQSPLAHRDSVSSTGSFATTSNSENPFETGPSHPYGMYPQHTVARPSSIATTSTERQPHRSMSLQRPTHPYAMYSQSGIADEEPPEEPARPAVPPIQTAIPVGFPGLNNGYHRVLGPDGEEQDIIGPDGHTEQLPPYSRFPEEGPTKASLAAEASASRVLPAPIQVADAEDPFTTPVSPVSPLSVHSFSAPALPSAPLLPSVTPARLPPQRPETQTGHAAPVSSTQANTVPAEPSDSSSASLLTRETSYSEKAEPTEVRSSWRKRKLWGKIPMGVAVFVLILMLVFAVALGAAIGTVVAKKHGNKDKDRNKSHEEPQAQVTGPNGSLFDAIPISTPAGLSALPTGSFSLPLGIPQESNPGCLTQANQYSAWSCKMTFAPLVITINNSSDSDGPQQFASMMEGPAIPDGSILYGLQTPKLDSKQMQLVLDLDYRAYGPAYHFATRYDKLVILRPEEMSLTTGSSTPAKRQDDKGFRQRFEVRPGDYPWYCFWNSTYIEGYIYSEDNSTAASFTAFPTIATTSSPSATSVDSAAVGVATSASATSTAGVNTTPTQPAQVTASASPVARRDASTDAAAPSRMAPYPRIIKIEERRLPNSPQPYCQQMVLLDNGSITPAPNGNDSPVRIWLQEQDPSYEEYFSAQATSTGGPTRKSKRELALEKQRRSDPGDACHCQWMFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSF6</accession>
    <name>A0A9W9CSF6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1996 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000914</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSF6"/>
    <dbReference type="OrthoDB" id="74764at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018535">
        <property type="entry name" value="DUF1996"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43662">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43662:SF6">
        <property type="entry name" value="DUF1996 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09362">
        <property type="entry name" value="DUF1996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1996" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4378083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="104" mass="11450" checksum="EA7F4E6206DD9F47" modified="2023-11-08" version="1">MLEIVWDTRQFNNKADWPADGSQPFYLSTGDNTGYGQHGDYVFGWKDDSLQKAMDDAKGCMGANCGSLKTQQPADGNACSVKGRVQEDHEGWLKELPGMDGMPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXC4</accession>
    <name>A0A9W8XXC4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_011001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXC4"/>
    <dbReference type="OrthoDB" id="3792750at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040930892" evidence="2">
        <location>
            <begin position="18"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="232" mass="22914" checksum="ACBF3FA6AFF7B5D6" modified="2023-11-08" version="1">MRYTIAAFAAFASLAAAVPQYGYGAESSATPTPTPEYPAYPVASSSAPAYPAESSSAEYPAYPVSTPAASSEYPSYPASSSVEYPSYPMSSSVPAYVASSSVEYPSYPESSEVPSYPASSAPSVPEASTSCLSSTTVTVTVPYPTGSGYPVGPGGKPSGPAPYPIVPSGTGYSAAPPAGTGYSVAPPAGTGYPTSTGGYVKPSSPAEFTGAASALNAAGFVAGVGAFAAFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZE7</accession>
    <name>A0A9W8XZE7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010793</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZE7"/>
    <dbReference type="OrthoDB" id="5120271at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015141">
        <property type="entry name" value="PLipase_A2_prok/fun"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09056">
        <property type="entry name" value="Phospholip_A2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="231" mass="26591" checksum="07DEB4EB8B6DAC37" modified="2023-11-08" version="1">MFPFPNAVKDACNRHDFGYRNFKKQNRFDDRAKEVIDQVLKSDLYRQCDHEIPRGACRFAAYIYWEGAVKFGNHHPQGSYFVNHAMASLATELEAWKVDHPECKYEEAFKDDCGKMDVRQGVQVEARQEEEVRTPKNVIAGLVEFYKSNSLADAERDWIPEWTRNHNTTNRTVVKKVEGVPIVGEASDNKTTEDPKDNECGEAETSDPETSDGEGDEWEAWDKEKPGEEYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZX7</accession>
    <name>A0A9W8XZX7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosome subunit biogenesis protein NIP7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NIP7</name>
        <name evidence="9" type="ORF">N0V83_009792</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with pre-ribosome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NIP7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZX7"/>
    <dbReference type="OrthoDB" id="27490at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21146">
        <property type="entry name" value="Nip7_N_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21151">
        <property type="entry name" value="PUA_Nip7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.10:FF:000002">
        <property type="entry name" value="60S ribosome subunit biogenesis protein NIP7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.220:FF:000001">
        <property type="entry name" value="60S ribosome subunit biogenesis protein NIP7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040598">
        <property type="entry name" value="NIP7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055359">
        <property type="entry name" value="Nip7_N_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016686">
        <property type="entry name" value="Ribosomal_synth_fac_NIP7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005155">
        <property type="entry name" value="UPF0113_PUA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17833">
        <property type="entry name" value="pre-PUA_NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03657">
        <property type="entry name" value="UPF0113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017190">
        <property type="entry name" value="Rbsml_synth_fac_NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88802">
        <property type="entry name" value="Pre-PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PUA" evidence="8">
        <location>
            <begin position="98"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR017190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4363497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="185" mass="20453" checksum="16DE5BC4F2EF400A" modified="2023-11-08" version="1">MRPLTEPETKTLFEKLATYCGKGISNLIADRAGGSDRNVFRVQGSRVYYVRESLANLATSVARDNLLSLGICLGKFTKTGKFRLHVTALSVIAPHARYKVWVKQNGEMPFLYGGNVLKAHVGRWSEDCPEHQGVVVLSMNDTPLGFGVSARSTAEARKLDPTGIVTFRQADIGEYLREEDTLFTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y056</accession>
    <name>A0A9W8Y056_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_009039</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y056"/>
    <dbReference type="OrthoDB" id="2406834at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF81">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="41"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="88"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4364445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="628" mass="70544" checksum="4E2F1665AD2B264F" modified="2023-11-08" version="1">MDDDGTQPSTSPDPPASARSASQAHDLHPRKKQKRNKPTLSCEECVERKTKCDRSRPHCLACIKRQSTCRYSEIANLIVSSADKVGAARATGRSRKQSSKPTLGNHTQPHALPVQGHVQVPPTPIRTQYRSVSLSSSGSSPFLLSNIPYSNHTSTTFFGLGSEHPFANYWTSRGGLAEVVGVLPAKEQADILVDTYFDAVDPVYPMVHRRNFYADYQRFWSLSLEEKQVADPVLVALHFVVYAMGTQFIHTPSNHERAQIAEFYVSAAQQALRLSSYLSRASVRTLQAMVLIAYFLMNDNHASDAWAFGGILVRQAYAMGLHRDPDIIAPRCSRSDKQQRRKLWQAIFFQDTFLTVLLKLPPTTTFSDIEPESLSDDLDDYLSNGSSSNGTTDPVINPMSISNIAQHDVYPAYELSDRRYIRSMWHLANLVSRTVCTPRSLATPLTNSVSHKEQLVADYYNLHSSFPAALTASDDATIRQMAETNPRGLRQNLFFRSNFWHCVMAIQADENTREGVVCDVKGTLEAARMALQSFFHFWEFLKIDASVWWVFQHRAFEEALLLARILGQQEQPLSPSHNGMPPSSDPLLHLAKQDARKCLEILDLVGIAPEMQKTRTEVLRTAFADILW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0E3</accession>
    <name>A0A9W8Y0E3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_010202</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0E3"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000433">
        <property type="term" value="P:carbon catabolite repression of transcription from RNA polymerase II promoter by glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051007">
        <property type="entry name" value="creA/MIG_C2H2-ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47428">
        <property type="entry name" value="REGULATORY PROTEIN MIG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47428:SF1">
        <property type="entry name" value="REGULATORY PROTEIN MIG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="28"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="275"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4363082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="306" mass="32498" checksum="4D7AB7E30F32937D" modified="2023-11-08" version="1">MLGKGQQHAAAHQAAVAAVQAGLMDSASSTAYMMPPPPKTISRSAPSSQLGSPNVSPPHSFSSYSPNMGNDLAAYRSGGFNSTSTSPNGLSRPMDLLADAASRLEQGPGHRSHSSRHHLTSAYHPYGNRLPGLSQYAYSSQPMSRSHSHEEDDQYAHRMTKKSRPGSPMSTAPPSPTFSHDSASPTPDHTPLATPAHSPRLRPHGFNDLQLPHLRHLSLNQNFVPALAPMEPQTDREQPYVPSQSSGLRIGDIISKPEGAQRKLPVPQVPKVAVQDLLNGPPSSGFSSGNSSATASLAGEDLSHRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0W3</accession>
    <name>A0A9W8Y0W3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009091</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0W3"/>
    <dbReference type="OrthoDB" id="7457040at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035965">
        <property type="term" value="P:cardiolipin acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF29">
        <property type="entry name" value="PUMMELIG, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="190"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="113"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="337"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="466"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="337"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="473"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="580" mass="65082" checksum="11D29A18C1C241C8" modified="2023-11-08" version="1">MLRTYLRTRPGLLHKTVGFGGASYISLSLSTPIRLESKGNATMASQEADVDAEVRRGQQSILASQRAKEQGEVQQGRFTKWFPLSAKAGFDQWWAGLSPMATEHRVLSFIPHLQSPPTHTETGSAPASTNPSSADLAAPPKHSEDHTTTNKSNDPYGPRRWNSEMVELGGKNRALNEFSVERLGEDVENNLVMLHGYGAGLGFFYRNFEAISRAEGWKVFALDLLGMGRSTRPNFKIHAKTKEERIQEAESWFVDALEEWRIKRGLERFTLLGHSLGGYLAVAYALKYPGHLNKLILASPVGVPEDPYAVNEDMPEPQDSTMANEFTQDAAENNNDVKASTADNNNFMNQQKKKDTAKAANQPPRRRLPGWLTYLWEANMISPFTFVRWSGPLGPRLVSGWTSRRFSQLPEEEAQALHDYSYALFRQRGSSEYALAYLLAPGAFARSPLIRRIQGVGRQWIQAHEAPTVDGDTPSTSQSNPSGQQAQRETGLPIIFMYGENDWMDVAGGFAAEEKLKAEKERILANASAEEKKRENGETKVVIINKAGHHVYLDGWEQFNRVMLEEMEDVKRRERQRSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y162</accession>
    <name>A0A9W8Y162_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peroxidase</fullName>
            <ecNumber evidence="14">1.11.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">CCP1</name>
        <name evidence="16" type="ORF">N0V83_008828</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2 Fe(II)-[cytochrome c] + H2O2 + 2 H(+) = 2 Fe(III)-[cytochrome c] + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:16581"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.11.1.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Forms a one-to-one complex with cytochrome c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion intermembrane space</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peroxidase family. Cytochrome c peroxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.-" evidence="14"/>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y162"/>
    <dbReference type="OrthoDB" id="2859658at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004130">
        <property type="term" value="F:cytochrome-c peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000302">
        <property type="term" value="P:response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00691">
        <property type="entry name" value="ascorbate_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000009">
        <property type="entry name" value="Ascorbate peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000005">
        <property type="entry name" value="Cytochrome c peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044831">
        <property type="entry name" value="Ccp1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002207">
        <property type="entry name" value="Peroxidase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31356:SF58">
        <property type="entry name" value="CYTOCHROME C PEROXIDASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31356">
        <property type="entry name" value="THYLAKOID LUMENAL 29 KDA PROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00459">
        <property type="entry name" value="ASPEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9 14 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 14 16" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="15">
        <location>
            <begin position="116"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4365210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="378" mass="42340" checksum="857BEF755B79A681" modified="2023-11-08" version="1">MASASRTFTRALRTATPSFRATSTRSSARFVAPTQTAFRQQWQRGYASGKETEDGADKYEGNPTGLIAGAIALLAGAGAYGLYTQKPELFGQEGKKKVFVARFEDYQKVYDAIAKRLEEHDEYDDGSYGPVLLRLAWHASGTYDKLTNTGGSNGATMRFAPEGDHGANAGLKAARDFLEPVKEAFPWITYSDMWILGGVCAIQEMQGPKIPYRAGRSDRDVSFCTPDGRLPDASKDSGHIRAIFGRMGFDDRAMVALSGAHALGRCHTDRSGFSGPWTFSPTTLTNDYFKLLLEEKWQYKKWNGPKQFEDVKTKTLMMLPTDMELVKDKSFRQFTDLYARDNEAFFNDFRDAVVTLFELGVPFQQPEDQRYTFKSSFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1F6</accession>
    <name>A0A9W8Y1F6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_008937</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1F6"/>
    <dbReference type="OrthoDB" id="5297881at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050815">
        <property type="entry name" value="TF_fung"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47338:SF4">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47338">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4365317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="234" mass="26298" checksum="6C612308F3D8F11D" modified="2023-11-08" version="1">MAGGVKGDSHFPWHSLSNLSKIRQELDIWASETQDTFTSIEALFGQPDSTILVLSKLIYHLIHCLIYRPFLPVDLAELSGTSQHQPWQIEATNLCFLHANAIAELVEIGRASSIIDWPAFVGYCVCTAGTIHVHGAHYPGREGEVFSVSAEFLSREMQQLSELRFIWAGVQHQRDTLQTIYGCHTELVQSLATNPMRYAPVFQLEDFFDRYPGQVFDGAHVTFLEIAMDSIQEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1I8</accession>
    <name>A0A9W8Y1I8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Eukaryotic translation initiation factor 5A</fullName>
            <shortName evidence="8">eIF-5A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ANB1</name>
        <name evidence="10" type="ORF">N0V83_009254</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Translation factor that promotes translation elongation and termination, particularly upon ribosome stalling at specific amino acid sequence contexts. Binds between the exit (E) and peptidyl (P) site of the ribosome and promotes rescue of stalled ribosome: specifically required for efficient translation of polyproline-containing peptides as well as other motifs that stall the ribosome. Acts as ribosome quality control (RQC) cofactor by joining the RQC complex to facilitate peptidyl transfer during CAT tailing step.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="8">eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the eIF-5A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1I8"/>
    <dbReference type="OrthoDB" id="9975114at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045901">
        <property type="term" value="P:positive regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006452">
        <property type="term" value="P:translational frameshifting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04468">
        <property type="entry name" value="S1_eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000007">
        <property type="entry name" value="Eukaryotic translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000034">
        <property type="entry name" value="Eukaryotic translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001884">
        <property type="entry name" value="IF5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048670">
        <property type="entry name" value="IF5A-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020189">
        <property type="entry name" value="IF5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019769">
        <property type="entry name" value="Trans_elong_IF5A_hypusine_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00037">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11673">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 5A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01287">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21485">
        <property type="entry name" value="IF5A-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003025">
        <property type="entry name" value="eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01376">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00302">
        <property type="entry name" value="IF5A_HYPUSINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="7 8" id="KW-0385">Hypusine</keyword>
    <keyword evidence="10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Translation initiation factor 5A C-terminal" evidence="9">
        <location>
            <begin position="87"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4364657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="161" mass="17581" checksum="D74F3E3077C87A53" modified="2023-11-08" version="1">MADDAQHEHTFESADAGASTTYPMQCSALRKNGFVVIKNRPCKIVEMTTSKTGKHGHAKVHLVAIDIFTSKKLEDLCPSTHNMNVPNVRRQEYQLLDVTDDGFLSLMSDDGTTKDDVKVPDGEVGDKINKLFSEDGKDTNVIVLTAMGEEAAIDAKEAPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1W5</accession>
    <name>A0A9W8Y1W5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enoyl-CoA hydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_008390</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Mycotoxin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1W5"/>
    <dbReference type="OrthoDB" id="410701at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000058">
        <property type="entry name" value="Enoyl-CoA hydratase/isomerase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF171">
        <property type="entry name" value="SD19268P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0843">Virulence</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4365769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="31817" checksum="952F4BA1A62407A9" modified="2023-11-08" version="1">MPLLRPSPRLLFRPVQRCAYATAQAPAVNVTNVPAPHCGSIRILSLNRPSARNAISRQLLAELNHQVNSIHDEGETGSTRALILASDVDSSFCAGADLKERATFAQEDTANFLASLRGTLRSISQLPIPTISALAAPAFGGGLELALTTHMRIFASNTIVALPETRLAIIPGAGGTYRLPALIGLSRARDMILTGRRVGAPEAYFLGLCDRLVEITPEEASQESASRKKVLDEAIKLARDICEGGPIAIKAALTAVEGCALGEKAENAAYDIVLKTQDRDEALVAFREKRKPEFKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1Y1</accession>
    <name>A0A9W8Y1Y1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1Y1"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040795177" evidence="2">
        <location>
            <begin position="25"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="88" mass="9813" checksum="BF5F48F438DC51C1" modified="2023-11-08" version="1">MLLKHPTIITLLLLVATLITLTNAKDHSKKTPDKHDPNCGAPFCNRGRLVPAAQVAQANEEKQKREEEDQDAARRHILEKVVPRALEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y266</accession>
    <name>A0A9W8Y266_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MFS general substrate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_008467</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y266"/>
    <dbReference type="OrthoDB" id="6509908at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF234">
        <property type="entry name" value="MFS-TYPE TRANSPORTER DBAD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="311"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="366"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="447"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="484"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="484"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="507"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4365846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="527" mass="57972" checksum="C2BDEA5FBE78F79D" modified="2023-11-08" version="1">MRTLSTPVSLEEVNEPDWPRSWRAYACLLGCFFLMFNSWGLVNAYGTWSSYYVGHSLRGVDQLELNLIGSTQSFLVLLFSNPVGRLLDAGYARTVIGCGTFLVPFGLFILSVVHPSDIAAVSSFGPIWATQGLVVGLGMGTFFVSSSQVASTWFPKRRGLAVGYVACGASVAGVVYPFMLRSLIEALGFNNAVRCVAGLTSLTCIYSFIFCTPDPAHDHHEPKRWRALRTWFDTEAFHNKAWCWFTAAIAFMFFGFYPIFFNLEEVSTPYLDLALQPLINHQWASVRGFGIRDRTSASATSMNVNDRPLETFWLLAMMNGASTFGRLLLAHFSDKIGALNMHIGSQFVASLLTLILWSLAGSENDAIAFCVVFGVFSGMVIGLPPASIGHILNCTYMTADTKHLAKKKLGHWTGMMYTFAAIPALTGPVIAGHLITQYNTYITVQMWSGTNLMLSFACMLVARWYLPCTNGRNAPSEIARIVRRQKEPLTEKGKGGTETGSPEALSQAPTRVPSNVPSRQVSTDRIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2F5</accession>
    <name>A0A9W8Y2F5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SAGA complex subunit Spt7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SPT7</name>
        <name evidence="11" type="ORF">N0V83_008338</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2F5"/>
    <dbReference type="OrthoDB" id="21449at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05510">
        <property type="entry name" value="Bromo_SPT7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22927">
        <property type="entry name" value="HFD_SPT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000072">
        <property type="entry name" value="Transcriptional activator spt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000032">
        <property type="entry name" value="Transcriptional activator spt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006565">
        <property type="entry name" value="BTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037782">
        <property type="entry name" value="Spt7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47343">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR SPT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47343:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR SPT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07524">
        <property type="entry name" value="Bromo_TP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="338"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="84"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="437"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="484"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="540"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="650"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1049"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="98"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="127"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="437"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="449"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1118"/>
            <end position="1139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4365718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1150" mass="127330" checksum="D257AE2D4098E7B9" modified="2023-11-08" version="1">MSLAASTHRPSSRERPRDARFLHQPSNATRRRTPNAALSDEAMSNGSNANNPGEGTDSIAEEDPRAARWREHYLRTEARLNAVLGGISQNDSPDASDTDPKPSSDDAPTAHDARPAITPKKPTRTIDEDDYGDDDDEDDDEDDTRTSPLLSKSAPNGIAATAPGPPSLMRIPSISNRLGLERTSTPATEQGKTSDDVRKKLQQDKKAAEDAAKRSFHTLFYTLESDRDAMMEQQKLDELDRQVEIETSGQSANAPANGAVAAPQQGTLSTTNLGASSLTLKHLISRIDAQRDRVHATDAQLRSLISEVRKNRSKWANEDRVGQEELYESMEKVLMELKAGEHAHPFLQRVNKREAPDYYNVIKHPMDIGTMMKKLKQLQYKSKKEFVEDLMLIWSNCLKYNADPSHFLRKKALHMKKETEKLVPLIPEITVRDRAEVEAEERRMRNGDVDADGADDSEDGMSSSICDGHDLLICCIEEPIMASRGRKAPSKGGKGSGTARKAPPAGMEDTPGPETKPPVPSINNAVSNLKNEFLRADSEMEGSVNGFSTPPPGTLTPLGPNGVLRNGAHGSQADNSEADGTGASVNGYAMEEDADLDDLEYKTWKQVTKKDRATIAAERHRLFRDDQLQADEPAILRTKAGMRRWLRHRKQAMEEESSAESKSAPDGKDGVNATAGESLAEGIEGEEERQVPDYYDVVCAIPDLTERLQWVEDAEGYAVQQAEEYMRIVPPGQFISPDGALAKKMDSNLQSLQDTRKICAKIGIVKQMQIQSQLYQNQFQRYEPQPFMEADIEPMVVSEDGPVMAPYVCRAALQRSVGKIFYHAGFEEFQPSALDAVTDIAAKFFQNLVASLGIYRETPKMKVDLPVSLPSGQMTSWIPRFSQEEAVLHALHANGVDLETLETYVKDDVDRLGTKLAGMHDRMRSYYAELLRPAMDNAGADGAGAFNDGSEQFVGGDFAEDIGEDFFGFKELGLDREFGLTFSVPLHLLQNRVHNAYARQDNNNVTTTGLLVPEPPKFEPVTIQAVESQIGLVQGWLLNKLHDNNSQALIEDDDLPPKQRFPKPRLPPTGKISSPRKRPLREQQQMARKKRKLDEEKDDNGNDQGGFMKSLGKPIGKLKLEPAQKENHSVQEPEKEDGSAHGMPSPPESF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2Z9</accession>
    <name>A0A9W8Y2Z9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2Z9"/>
    <dbReference type="OrthoDB" id="5326346at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="26736" checksum="E1EC33A0499C5850" modified="2023-11-08" version="1">MVIEHDATTIDEGSETEQEEPPSEHGPSEEPDYVYELSHMDAQVFITKRTSAKENSDEKSDQHQSDIEPTDDTEPTVEYQVSSKHLIMASGKFRSELTGPWAESTKGEDGLHHLTAEDWEPEVFTILLNILHLQNRQVPRSISLEKLAKLAVLVDYYRCWEAVEMWTDIWIAEVKVQAPVPVSYGRNLILWMLVAWVFELDEEFEKTTAVALRQCQTPLINNMELPIPEAVIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y338</accession>
    <name>A0A9W8Y338_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007301</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y338"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF8">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="94"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="355" mass="38618" checksum="FE38F2D32B8B2990" modified="2023-11-08" version="1">MSLDFSQYGGPSEEWLAVQKTLPAMTFDVNADPVALRSAVNTERENRTAAAFIDLAAQVETADYSIPTRDGSSIQARTYRSVEKSKSEKLPVYIYFHGGGFLFGTLSSEDFTCAQTAINTGVIVLNVNYRHTPEHVFPTAWHDSQDALAWLHKNIDTVGGDPTKVLVGGVSAGGQLAASLVLEQHLGKSEVLKGLPAVAGQILIIPCVAHPETYEQGPGKKLKVSSYVENEHAPVLPKTTIDFFMGLLKVDTPDFKDTKVNIVNATEDEVKGLPPTVFGIAGLDPLRDEALLYAKLLAEANVPTEIRLFRGVPHGHRRFGKALKASEHWDQCIDEGILWALSKPTATGKFDVKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4E8</accession>
    <name>A0A9W8Y4E8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyl transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007882</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2582433at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12526">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12526:SF630">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13692">
        <property type="entry name" value="Glyco_trans_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="874"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="911"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="951"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="982"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1016"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1041"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1236"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1272"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1333"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1360"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1389"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2903"/>
            <end position="2973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2988"/>
            <end position="3035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2913"/>
            <end position="2934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="3011"/>
            <end position="3025"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3078" mass="348366" checksum="E179FB22861A2B4E" modified="2023-11-08" version="1">MPEPDILGIQAFRTGPLLQEWWEWTLVAIISASGLVGVLSFVWFSYRSIRNAFKANKDEYLLPRSVQERIHLLSNSSNVIRIRQNLPAKPASFGVYLGLLNNPVTEEEAQILSQWEAVVLDYCEPGVLEAVSDDSIPLGPHIIARLDLLQIIKFAAMDNELDMARTVYIISWIIQQTLRRPDQRRYFTGVLVAGWRERMSAPLLNGLAKLMTAYGLEVYLEVGPPNFLDGVEKLDIKLFSGIIVRNGTIMPNGERRDFFDMDKMKTTTKAFVSQACQRPFITMMWDTINDDAALSHAVVRRAHMWCNYHGAVPYFTRQRALTNVHDVRSCEEPLAAFQWLKNKRVMSVHETYRTTRTLSAGFSSIIDDYLPLQGIFPLLTGTLAGLDGLESDDDDASSTSTLNVHYPEIDENGVLLPMSPASPVSQGLDWTVALEKRASNPLSCSFDGALYGPLGCFPIGLNATKADFDHVLKSQRRLRDLRLLSRHPVTQLHAAANVLSHYSLDASSFLDSVPNSREAISNLADALAKTTDEGDDPYEIQVYSGLDSGFHTPSGAQFWSVWEVEPRTTSIIIYVSKSVQDLTGVLLHTHLSRLGFSRYQSFLLEYGLNESQAETRPLRGLPQRLKQDLNLLSSSDLLLYLQHIQYSDWNEECLLLSALREQCEELLIDVPTYQQFKKSSNIDYIGDMITDEQLVNAKLKWYRLCHLPTLDRHDALELFRHISETFSDILWWRDHERLDTITSAIEDISSRESLDSVADFVLFCIFCAARKAGFEEVYIEVSDRNPLFNQYSDQSAAFAELFALGSRCEAYFDIKPSDIGMLLSKKHRDYYNQEEHQPPMWIFNAPSFASAYAAAQTDIDPNQKASVMPAYRRFTFLSVFAIPALVDIILLSTTGHGLYLSVRMTEPEQKYATLALMCSLLLSGAIGTWISIGGTYYLISMAFSAANMFVLTRLIGGLAFTFAGCFLGFVVISAVVGPELGAVFFFYLLGLTTYLSVLAVLSTYQVPGSSFLNGRRVIIMVVPTLIVSPILTIFFPGHDIIIYPGILYIFVILLILGTRHVATQWVTWYHNIKTLNDADVKDWYIQRHIKRERAVNMDKAKRLSKGPSFSGALRDSVAPSSEMSFGEPDIFQDLSEPAILALCRKDLYNAVIKETERHFWQKPTEDAFVKRLADCWESTLFLLDWYCRTTDTKRPIPYSSTWNLETQVALESMQQSQKGIKLHNSFIHWRTAGDEIYCGILYFLVALMDRWLDLVRGGQLVGLASNMNTTLRLSVGFGLAYYLIGAVLLDYKAQHLHTLSEQMSPVSIENGPQIRKAKANDQKFRRHLYFNTLCRFIGVHVWALALCAALIWIFNGSSTGLVMFLAYVGSYTGLLLYQYNKIFSGPHALMPLLAAVFLGIVVGNILMEVFPHSLYDKVIALAVSTWTAAFLSFRTAKLGLPEVFDIRLWLSESLPTLKRSKKPFESTESVNIKGIPSQLRIHWSTNEKYHAYNGAGTDEEFSQSELQAFCNKLRAAPKEDRFRVMPTGHPGDQITAILLSCTHDNLTRLALHAFPTMPFLVQRIVLAWKNDLIKIFIVPMQSVAEVSADLRAISQYSDGRLTLYIASDAKGSASGQMNVSSNCTTIAETLLHACSETMFGMPHSQAEITESMLACRPVSNELHAVSECNKRSMPALVSGPEALAFETTCRKDLLRNLCLGYDCETQWDVLPLDIRRLFFRRCLGARGPYTSTEMSWINANVPAEDSCTILSRVARYDLGAYLAVNKLNYFMERGDQALSEKEYRQMTADIQEAYHPVLNRSAVTLISIFTEYVRIPLTYVYHTAGTWVKFFVLACMADPEYQRELNGALIRTPRILAKPATWILTGLWIYSRIAMSITLPFFLYHHRKDIAGLAGTVKGSLIIQKKNRLIIRSYGDTETAFVRPIENHTFSLTFYQGTPEQEPTWGHTRITRYDNEMRVTLRQEYKNGQVANEYAYEYATKRPERKIKISKIKSSRIPLSRSCISGSDEGAKVFYNHKGHIESGSYINHGNLVRFKYHYRKNAKYDDELLRAEFVLPHMSANVSWCAPPVRHAEKMERWIPTPRVHEATFVQGADVYECTWLYDHKFHPTITTKLNGESVETPDMIRHDWLGVLKKPTRCTFTDENPLLAFKAPTSSFMSRLFRNNIKRQEISTSRARSQLWKAWKKRNDLDGVVIRWVDEELIRKEALLRPYWRRRDRGSLLKAEDYLALHADAIMASSDLTSDISAWTPLAIRMSDLFSFGQGGDAVIFTRTKALQRDTDRNLHVIAVDTGTWPNEGGGVSACRRDLINNLRTIKWHMVVESANDFGLPKHQTEENVESLKVIPLWGLDFMHPSHGMFTNKLDSEVDHLVKETTIADIKMNFIPTLTALVRGARAINMTAADVKQATRALMNLNTYFQDSRHWKEVWTSDIVKDTWRKLWLADDMPNAQPPSEWFRTELPTLGHFDTALELWFRYLFIFSIPIPEKIPNVFQASHHSVSASYGIVCKLKRNCTLQIWDHAISWRETNLYLSSAMCTLPPFIRNSLLGLMKLTSCLILHHADQILPCADFFNPGWEVEIGSAKGQLTHRNVFRRKVDPIVNGITDMQKFAPVTEIKTKTPTVTMLSHVWFAKDIKTALLAADIISNEWGFKDYKLDIYGALNKSPVYSSECQEILACKGLGPSVTLRGTADPAMVLANSWLFLNSSVSEGLPLALGEAALTGAPVVCTDVGASLRVLTDPDDGKRYSEVVAPNDAYGLARAQINLLAMLDEWAQYADDKPGESAPILPPKPTAKDVEVITRRMYEKTEHRRKLGMMARSIVQKSFGGERYLREHEQMLWIGKACYEMLGLEKRTPPPRNPARMLSVRHRRRTLHEQQQYQHQQHTDTASSNMIDPQMEKMLHPQRPHAPRRTSATTSFSSVYIDQPSSSSTYSDPIWEHDGEEDYWEGSVDAYSPNSSTKYPKSERSGEDIWPSPVIMRPLKTYSRGGGGGNAVQAAIPKSPTSPSSRVARGKRPARHDHHYHHGYTTPINPKMDPRRAVLMRQSMAGPESGRSSVRTRSHLNEVEVAEYGNVDGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4M1</accession>
    <name>A0A9W8Y4M1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kynurenine formamidase</fullName>
            <shortName evidence="3">KFA</shortName>
            <shortName evidence="3">KFase</shortName>
            <ecNumber evidence="3">3.5.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Arylformamidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">N-formylkynurenine formamidase</fullName>
            <shortName evidence="3">FKF</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_007031</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>N-formyl-L-kynurenine + H2O = L-kynurenine + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13009"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58629"/>
            <dbReference type="EC" id="3.5.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="domain">
        <text evidence="3">The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the kynurenine formamidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.9" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4M1"/>
    <dbReference type="OrthoDB" id="420264at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03014">
        <property type="entry name" value="KFase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027519">
        <property type="entry name" value="KFase_ver/fungi-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF33">
        <property type="entry name" value="KYNURENINE FORMAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0823">Tryptophan catabolism</keyword>
    <feature type="short sequence motif" description="HGGXW" evidence="3">
        <location>
            <begin position="48"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4367448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="322" mass="36113" checksum="A5C3DABC9121364F" modified="2023-11-08" version="1">MTSTTYPRLIPSISYSDSPSRLQTLDVWLPRPLESSDTAKSLWIIYIHGGAWRDPLQDSKCVEPTLKHLFSEPNASALSHVAGIASLNYRLSPYASHPTEPGSPSDQDRNAKHPTHVRDIARGIRYLQKEYGVKRWIGVGHSCGATMLLQLVSGIGLEGDHNSSLSSNAENRGPEALLLLEGIYNFPLLLRNHLPPTCPENISQIYRDFIAGAFGSDESVYQAVSPVSGRYNKEQWPEGRLLVICHSYEDELVERAQRDVMCVALDREGWSIVMEEGDEEDEVRAEGRRVLNVRDMKGGHDWVWEDGEQIARLIGEVVQRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y569</accession>
    <name>A0A9W8Y569_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerases I, II, and III subunit RPABC3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RPB8</name>
        <name evidence="5" type="ORF">N0V83_006507</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the eukaryotic RPB8 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y569"/>
    <dbReference type="OrthoDB" id="20018at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000191">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005570">
        <property type="entry name" value="RPABC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03870">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000779">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00658">
        <property type="entry name" value="RPOL8c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4368151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="146" mass="16602" checksum="29D19C30DB5786A7" modified="2023-11-08" version="1">MADAQVFEETFSITSINNEKYDRVSRIFGTSTDNQITMTLDINHELFPVQTGETLNMVLATTLSLDGTSKEQNETMWRSVGKHGVTTLADMYDYVCYGKNYRMEDGDGDQMKYYASFGGLLLYMEGPYRKLGGLKIDNIYMLVKHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5V7</accession>
    <name>A0A9W8Y5V7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tat pathway signal sequence</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007991</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5V7"/>
    <dbReference type="OrthoDB" id="5296155at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="164"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="410" mass="43719" checksum="43652FF53A99B163" modified="2023-11-08" version="1">MDFLRSKRSSTHQPIGDERPAKRISTPAGQRLSVILDSTRSKSPSATSKINVTTTSTYCNDPTHLHIGPVQHGSKGARKSGMVAALTGGRLGDTPRESSEDQTPNGSNLSVSVWSDKDAEKFGRIRQRRGIAGWSWKRVAILAAMLVALIIALAVGLAVGLKKKPSSSLSSTPSTTTTTENTHPAGSDSSTSTQTSAPSSSPTSTLTPSAAPANFPVGSYSFVTFLDTVSTGCTANNSTWTCTPFTDYYTDPQKALTVLNWEITGTSGSYKISSNGEDGTFGTTFQNEKLQLLDEGKDAERYRFQITRSKTVNMTGSIGNEKGDFECDFGSTNLNGFLYTKMQRTYPDDTIAVSGASSNVWPYAVRVEQTVAGGENVPSCKSSDGDSVGLEAQDASTLCSCLYKNWTPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y653</accession>
    <name>A0A9W8Y653_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoglycerate dehydrogenase</fullName>
            <ecNumber evidence="4">1.1.1.95</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">MYO2</name>
        <name evidence="21" type="ORF">N0V83_007579</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(2R)-3-phosphoglycerate + NAD(+) = 3-phosphooxypyruvate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="1.1.1.95"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.95" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047545">
        <property type="term" value="F:(S)-2-hydroxyglutarate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15480">
        <property type="entry name" value="fMyo2p_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01380">
        <property type="entry name" value="MYSc_Myo5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12176">
        <property type="entry name" value="PGDH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000041">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046943">
        <property type="entry name" value="Fungal_Myo2/2A_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036103">
        <property type="entry name" value="MYSc_Myo5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008759">
        <property type="entry name" value="PRK11790.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF706">
        <property type="entry name" value="DILUTE CLASS UNCONVENTIONAL MYOSIN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50084">
        <property type="entry name" value="Myosin S1 fragment, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 15" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12 15" id="KW-0505">Motor protein</keyword>
    <keyword evidence="11 15" id="KW-0518">Myosin</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ACT" evidence="19">
        <location>
            <begin position="412"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="20">
        <location>
            <begin position="501"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="18">
        <location>
            <begin position="569"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="domain" description="Dilute" evidence="17">
        <location>
            <begin position="1718"/>
            <end position="1987"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="684"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="15">
        <location>
            <begin position="1150"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1503"/>
            <end position="1555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1597"/>
            <end position="1616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="684"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1503"/>
            <end position="1543"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="663"/>
            <end position="670"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ4367049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="2082" mass="234594" checksum="FAD23960117F7CFA" modified="2023-11-08" version="1">MTPPQDIAGSVNQLARTVSNSLNFSTSPTLSFHSPPNTFRQPPSGSFSNGRSGSIPGISAAVKQLKPFDTKDIKVLLLENVNEAGVDILREQGYQVEALKSSLSEDQLIEKIRDVQVIGIRSKTKLTQRVLKEAKNLIVIGCFCIGTNQVDLQTAAQQGIAVFNSPFSNSRSVAELVISEVIALARQLGDRSMELHNGTWNKVSKGCWEIRGKTLGIVGYGHIGSQLSVLAESMGMNVIYYDVLTMMGLGTARQVSSMDDLLEQADFVSLHVPATSETKNLIGAAELAKMKDGSYLINNARGTVIDIPALIEASRSGKLAGAAIDVFPNEPAGNGDYFSNDLNSWTKDLVGLKNIILTPHIGGSTEEAQSAIGVEVSTALVRYVNEGVTLGAVNMPEVNLRSVSVDKEDDVRIIYIHKNMPGVLRQVNSILLSHNIDKQMSVSRGEVAYLMADISDVKESEIRELYQNLEELSWSPEDPDIRRVESDERLPTPPTMAHIYDIGTRAWQPDTTEGWVASEVTDKQIAGDKVKLVFQLENGEKKTVETTLSAIQTGNDANLPPLMNPAMLEASDDLTNLSHLNEPAVLQAIKLRYLQKEIYTYSGIVLIATNPFARVDSLYVPGMVQVYAGKQRSYGAPHLFAIAEEAFADMLRDQKNQTIVVSGESGAGKTVSAKYIMRYFATRESPDNPTRRRGKSDSMSETEEQILATNPIMEAFGNAKTTRNDNSSRFGKYIEIMFNKQTDIIGAKIRTYLLERSRLVFQPLKERNYHVFYQLVAGASDAERQELSLKSVEEFSYLNQGSAPVIDGMDDVAEFKATRASLTKIGVPAETQAGIWRLLAALLHIGDVKITATRTDSSLSSDEPSLVKACHLLGIDANNFAKWIVKKQLITRGEKIVSNLTQQQAIVVRDSVAKFIYSSLFDWLVERTNESLATEDVLSHAHTFIGVLDIYGFEHFAKNSFEQFCINYANEKLQQEFNAHVFKLEQEEYMREKIDWTFIDFADNQPCIDLIEGKLGILSLLDEESRLPMGSDEQFVTKLHHNYSGDKHKFYKKPRFGKSSFTVCHYAIDVTYESDGFIEKNRDTVPDEHMEVLKASNNKFLSEVLDVAAQIREKENANTASAKAGAAVSAGRRIAVNRKPTLGGIFKSSLIELMHTINSTDVHYIRCIKPNEAKAAWQFDGPMVLSQLRACGVLETVRISCAGYPTRWTYEEFALRYYMLVRSSDWTPEIRNMATAILKKALGAGKNDGTDKYQMGLTKIFFRAGMLAFLENLRTARLNDAAIMIQKNLRAKYYRRIYLEMREAIISVQSLARGYMTRERAEEARQVKAATTIQRVWRGSKDRKQYTLVRNAVIKFEAAAKGFLLRKNLLDTRLGNAARMIQRNWRRQRWIREYQKKRKSIIMVQKLWRGKKARKEYKTLRAEARDLKNISYKLENKVLELSQSNGTLKQQNKSLQSQIENYESQIKSYKERSRTLENRQKELQAEANQAGITAAKLSQMEDEYKRLQTSHDESTAKMRHLQEEEKELRASLKRTTDDLEQSKRRSNVTESEKLSLRQQLAELQEQVELMKRSGPISELTNGHTPMAASSLINLVSSKKPKRRSAGPDTRDLDRFSQQYNPRPVSLAVGSTAHRQNLSGSTFAQLDNVELELENILAEEDMLNDEVTLGLIKNLKIPSPTSTPPPTDKEVLFPAYLINLVTSEMWNNGFVKESERFLANVMQSIQQEVMQHDADDAINPGAFWLSNVHEMLSFVFLAEDWYEQQKTDNYEYDRLLEIVKHDLESLEFNIYHTWMKVLKKKLHKMIIPAIIESQSLPGFVTNESNRFLGKLLQGSNTPAYSMDNLLSLLNSVYKAMKAYYLEDTIITQCVTELLRLVGVTAFNDLLMRRNFLSWKRGLQINYNITRIEEWCKSHDMPEGTLQLEHLMQATKLLQLKKATLNDIEIIQDICWMLSPNQIQKLLNQYLVADYEQPINGEIMKAVASRVTEKSDVLLLTAVDMEDSGPYEIAEPRVITALETYTPSWLQTPRLKRLAEIVSQQAIAQQEKMEFDDTNGENGVDGHDGNGLAVMSNGHETIEEGIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6A9</accession>
    <name>A0A9W8Y6A9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007103</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6A9"/>
    <dbReference type="OrthoDB" id="37537at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19077">
        <property type="entry name" value="AKR_AKR8A1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050791">
        <property type="entry name" value="Aldo-Keto_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625">
        <property type="entry name" value="AFLATOXIN B1 ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625:SF78">
        <property type="entry name" value="PYRIDOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="11"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="330" mass="35610" checksum="DEA666EC64773451" modified="2023-11-08" version="1">MPKIVGKDIGKVGYGLMGLTWRQSPPSQEQSFQAMRTALSLGSNNWNGGELYGSPERNSLHLLNEYFTKYPGDADKVVLSIKGGLVPGQFKPDGSKAGVQRSIDECLKQLDGKKSLDLFECARVDPTVPIEETVGYIAEYVKAGKLGGISLSEVSAASIRKAHAAHPISAVEVEFSLWSTEILDNGVAATCAELNIPIIAYSPLGRGFLTGKYKKHSDIEPDSSLHHFPRFQPDVFDENIKLLHAVEDIAKSKGATPAQIALGWVLAHSGAKGMPTIIPIPGATTAERIKENTTPAKLEEADLKALDEFLKKFEVKGGRYHPSGSALLFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6P9</accession>
    <name>A0A9W8Y6P9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">glucan 1,3-beta-glucosidase</fullName>
            <ecNumber evidence="10">3.2.1.58</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Exo-1,3-beta-glucanase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">BGL2</name>
        <name evidence="14" type="ORF">N0V83_006953</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1-&gt;3)-beta-D-glucans, releasing alpha-glucose.</text>
            <dbReference type="EC" id="3.2.1.58"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.58" evidence="10"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6P9"/>
    <dbReference type="OrthoDB" id="1293114at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050732">
        <property type="entry name" value="Beta-glucan_modifiers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631:SF26">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040872440" description="glucan 1,3-beta-glucosidase" evidence="13">
        <location>
            <begin position="20"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4367372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="304" mass="32472" checksum="B7B4E3605046A3E1" modified="2023-11-08" version="1">MRFSTVAAALAASPALVSAAKGTMGFALGTKVASGDCKTQQDYSDDFDAIKSNSGSTLVRGYAASDCDMAKNALPAAKSAGFKVVLGIWPDVEESFEKDLKAITDVADQYASTIYAVTVGSETLYRGNFTGEELKDKISTVKSALPSGIKVGTADSWNKFADGTGDAIIGTADIIMINAFAFWQGASKDNATHVYLDDMYQAVTHIEKVAGGADKMPEIWNGETGWPTAVGTDYEDAQGGLENAKYFYQHGFCSLLDWGFNAFYFEALDEPWKPASIGDNGNVADETTWGAMTSDRKAKFSLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6R7</accession>
    <name>A0A9W8Y6R7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">[NU+] prion formation protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">NEW1</name>
        <name evidence="11" type="ORF">N0V83_006140</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6R7"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18626">
        <property type="entry name" value="CD_eEF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000076">
        <property type="entry name" value="Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.990:FF:000002">
        <property type="entry name" value="mRNA export factor elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000193">
        <property type="entry name" value="Probable Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015688">
        <property type="entry name" value="eEF3_ABC2_chromodomain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047038">
        <property type="entry name" value="eEF3_chromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF14">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24984">
        <property type="entry name" value="HEAT_EF3_GNC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24987">
        <property type="entry name" value="HEAT_EF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0034">Amyloid</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0640">Prion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="8">
        <location>
            <begin position="119"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="485"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="729"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1036"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1042"/>
            <end position="1057"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4369057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1121" mass="123779" checksum="64B5C67B4835A2DC" modified="2023-11-08" version="1">MPHSESPPMAPAIVVDKAGAAPPPSHEDVTAFITSIVTAKTSHASVDAAYALTTVLQNSVGFRGLKGYGIIDEIKKAATNKKDAVRREGAMNALGAFFERLAREQKLTEAIFLVQEETLVPLVLDALADKTGSVKESAKYALDALFDNLTAEAKVFGLLPVLTKYLSKKSGKWQGAVGAYDLIGRMADKAKMGMESLEVEKEKDVLREAMGKKLEGLIPVVESGMHDLKSEVAKQSNKTMNSLTTLLQNDDIAPRIPLLVKSIEDPSTQSLQKAIHALSQTTFVAIVTSPVLAMVTPLLERSLNSPSTSQEVTRQTVVVVENLTKLVHDPIEARAFLPKLRPGVKAVVDRASLPEVREIGQRALDVIDKAMADRDGNVDSGAIARTQPSNVAETIQKQISVVGNMLNFPGDAEVWAVARNYISEMIAEDVDQRELKRIPQLIAPYLEPLMNAGDAEKVAEAVHQFYVDEDHRKFGQPVQEDDGETEIVNATFSLGYGGMLLLSHTNLRLLKGHRYGLCGRNGAGKSTLMRSIAGGKLEGFPPQDEVKTCFVEHNQGEDAEITILEYIVRDPRFKDESRERIIEVLEEVGFTSGPEGRQSEKVGSLSGGWKMKLALARAMLMRADVLLLDEPTNHLDVANVAWLEQYLQKHTEITSLIVSHDSGFLDAVCTDIYHYEQKKLICYKGNLADFVKVKPEAKSYYTLSASAVQFKFPPPGILTGVKSNTRSILRMTNCTYTYPGGSKPSLVDASCNLSLSSRVAIIGANGAGKSTLIKMLTGETIPQEGKVEKHPNLRIGYIKQHALEHVEMHMEKTPNQYLQWRYANGDDREVLMKQTRVLTEADKAQMETPIDLGDGKGPRKLEALIGRQKYKKSFQYEVKWLHMLPKFNTMISRETLLEKGFQKLIQEFDDHESSREGLGYRVLEPKVISKHFEDLGMDPEIANHNEISGLSGGQKVKVVLAGAMWNNPHLLVLDEPTNFLDRDSLGGLAVAIRDYKGGVIMISHNEEFVGALCPEQWHVADGRVTHKGHLAVNMDRFEDSRPGSSNVSSNVSSAAPSTTGTPVMSDAEGSKDDMKFRAKKKKKMTKKEVKEREVRRRLRYIDWLNSPKGTPQPPNTDDEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y743</accession>
    <name>A0A9W8Y743_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_005973</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y743"/>
    <dbReference type="OrthoDB" id="10254418at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046513">
        <property type="entry name" value="DUF6691"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007568">
        <property type="entry name" value="RTA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465:SF35">
        <property type="entry name" value="RTA1 DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20398">
        <property type="entry name" value="DUF6691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04479">
        <property type="entry name" value="RTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="247"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="323"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="368"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="405"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4368891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="452" mass="49283" checksum="0B2E6A232A3FA96F" modified="2023-11-08" version="1">METLAFFAGMATSYLPLKALAPQLITNYPSIPMTVQASLLTIGVGLIVGWGTKTSNGCTSGHMLCGLSRLSGRSTAAVATFFPVALITHHLVHPTIYTEACPGNLPCYTPIYPSIASTASLVLLAATSVLSARTIPKFIAAVMASGEKDGKERTDTRSVARSTTYFFSGLLFALGLHISQMSHPAKVASFLSFPSLQHWDPSLLLVILFGVVPNLIENQRKGFSAPPSFADGYSLPAKTFKDVDFKFVAGAAAFGVGWGLTAASVAIAVFSILFFYHTYRLFRTRTWFCVAFVVGAAFLIQAVGATILVTATEKDKMNRGKTIVLVGLVLQIIIFGFFIVVALVFHLRLRKVAAAGKQILCQLDWSKYLLRLYAVSSIITLRNIYRVVEYEEGVTGYLMTHEWAIYIFDALLMACVLALTSTWYLGKIETVNEDHVDGHNLVNRTPSEYTQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7B2</accession>
    <name>A0A9W8Y7B2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">pectate lyase</fullName>
            <ecNumber evidence="5">4.2.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_005707</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>Eliminative cleavage of (1-&gt;4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.</text>
            <dbReference type="EC" id="4.2.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 11">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the polysaccharide lyase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.2" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7B2"/>
    <dbReference type="OrthoDB" id="1637350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030570">
        <property type="term" value="F:pectate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000036">
        <property type="entry name" value="Pectate lyase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002022">
        <property type="entry name" value="Pec_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045032">
        <property type="entry name" value="PEL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683">
        <property type="entry name" value="PECTATE LYASE 18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683:SF18">
        <property type="entry name" value="PECTATE LYASE 21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00544">
        <property type="entry name" value="Pectate_lyase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00656">
        <property type="entry name" value="Amb_all"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040936320" description="pectate lyase" evidence="12">
        <location>
            <begin position="19"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Pectate lyase" evidence="13">
        <location>
            <begin position="45"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4369943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="315" mass="33091" checksum="B7E06121957F1D3B" modified="2023-11-08" version="1">MSLGLMAVSALAAPLSSTIVKRAASLEDVATTGYATLNGGTTGGKGGSTVNVATLAELTAAVKGDTASIVLITGPITGSGENVKIGSNKSVIGKDKSVVLTDFTLTVKDVKNVIIRNLAIAKVVGGDAIAVQVAQNVWIDHVDLSSDRDHDKDYYDGLLDITHAGDFVTVTNSRLHDHWKCSLVGHSDNNGEEDTGHLRVTYNKNYWYNINSRGPSFRFGTGHIYNNYYENVSDGINTRDDAQLLVQNNVWSGSKKPLYSTDEGYAVANGNDFGGAENTALAGTLTAAPYTVTDLLAASAVKAAVVADSGVTLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7B8</accession>
    <name>A0A9W8Y7B8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">WD40 repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RIC1</name>
        <name evidence="5" type="ORF">N0V83_007017</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7B8"/>
    <dbReference type="OrthoDB" id="67540at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034066">
        <property type="term" value="C:Ric1-Rgp1 guanyl-nucleotide exchange factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040096">
        <property type="entry name" value="Ric1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009771">
        <property type="entry name" value="RIC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22746:SF10">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR SUBUNIT RIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22746">
        <property type="entry name" value="RAB6A-GEF COMPLEX PARTNER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25440">
        <property type="entry name" value="Beta-prop_RIC1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07064">
        <property type="entry name" value="RIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RIC1 C-terminal alpha solenoid region" evidence="4">
        <location>
            <begin position="835"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1031"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1053"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1064"/>
            <end position="1077"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4367434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1096" mass="121864" checksum="D861BB070D19B4A5" modified="2023-11-08" version="1">MTYWPISSPSVFAATKHTNPERTHVSHDGVERKQQSQVEDETPTQGRSGVQEKNGIRLQQQSASEQSAEDDIHGKMIAIRVTRSGQMFATLTHSTLTIWQTKPTVVLASVLRSEQSLKTYGPNTDVLLRPDFQIFVVQTTLGFLITYSLATDHASRVYKTQFTNTHGGHSRRSSAISGFKIQRQHDRNAGPGEGNGLKELSLRFRMVIRVDAGIVRALALDDELVVATEKPTAIQCIRWAPDSTGSQTSTELLAKMPWVGKKATLRDMIHDRPMNLSCWITGEGQAFAVQRIAQREANASPTLFRGYGFHTPETDADYGVKAAINARFSLLAVGCASGEIYVYTARDYTGNIPLSHKLRPNTTSPGKLNVLAYSPDGYCLFAGYENGWTIWSVYGKPGATSFTTDRTLSETNGEGWLLGVQEAFWIGGGAELVMLSNNDNRLFVLEMARSAITGCFSSANISRSLMQTSTGFMIYRGYDLPDLTTISADVSLWHTVQIPSTYLVDQWPIRSAVISNDGRYVAVAGKRGLAHYSVNSGRWKMFDDPFIENEFTVRGGMCWFQHVLIAAVECHDSHEVRIYSREAALDNSHIMHIQKLPAPIVLIASSGEDSLLVYTYENILYHYVISVADAAVTLIQVGQIALHGIIRAPPRVRALSWILPENQIHNGDPSQDVAVATILFLVDGKLVLLQPTTTEGGELKYEMRIIAQNVETYALMRDHPAFALDMQHPDSLPPSPSIGLNINGVHSHDLRDSLWFFDGHDMRVWIDMQDVLSSVSAELGRELPTPIRIPVDFYPLSALLNKAIIFGVEPEIIQRRDTSFAFLRLGTRTHLFLPALLRYHLAQYNHPAALHLSHHYQHLLYFPHALEILLHEVLDEEVDTQPPPEQALLPSVLSFLSSFPQYLDIVVQCTRKTEVRSWRTLFLNLPPPEELFEESLQKGNLKTAGGYLLVLHTFEELRSTGDQVVRLLQRAKEEQDWELCKELARFLMALDESGATLRSTLELVELKSPSAEPGFGQSHFTFETSRLNVPVRGRSNGHRADVGLGRPSDAASEESRGRRPRENGSVSPSGSSVSPGSATAHEGLGDYFGLGMQNVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7I5</accession>
    <name>A0A9W8Y7I5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Class II aldolase/adducin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005812</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4370048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7I5"/>
    <dbReference type="OrthoDB" id="3238794at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051017">
        <property type="entry name" value="Aldolase-II_Adducin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672">
        <property type="entry name" value="ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672:SF39">
        <property type="entry name" value="CLASS II ALDOLASE_ADDUCIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="161" mass="17782" checksum="2ECFD8E04DC1DB6E" modified="2023-11-08" version="1">MASEIHKARPDLNAACHAHSVYGKAFSCFGRPLEMIYQDALRFYNDLAVYPRYGGTVISTEEGARIAKTLGNCRSIILQNHGMITCGATVDEAAFLFIALDRCCHAQMLANAASGPGWEKILIGKEEAEFTHKKSGNSGKMWLAFQPYFDQVAKTEPDVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7J9</accession>
    <name>A0A9W8Y7J9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_006794</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7J9"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000117">
        <property type="entry name" value="MFS hexose transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF29">
        <property type="entry name" value="SUGAR TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G14500)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="363"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="398"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="468"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="39"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="524"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="524"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4368437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="543" mass="61332" checksum="C6294277736A3351" modified="2023-11-08" version="1">MAIIKAQDGTSDPIITRMVEADKVPWYKKRNLRLMYLYLFCCCMGVEITSGFDSQLIGTLQFSTPFNKFFGDGYKDKDGKLSIEPSIIGFMSACYQLGSILAVPIAPWVNQKYGRRFSVMLGSVIMVVGALLQGFAQHIAMYIVARMLLGVGILFCIIAGSALIGELGYPKERPFLTSLFNASYFVGAILAAAISIRTTGIVGNWSWRVPSLLQICPSVLQLATIYLLPESPRWLVSKDRDDEAYAILVKYHAEGDANSVLVQAEMAQIRSTIKIEMEHSKQTWLDMFRTPGMRRRVLIAVFLGLFTQMSGNTLLSYYQSLLFGLMGYTSTYAKTRINIANQCWSLINAVVIALTVTRFRRRWMFMLSAGSMVMIFMAMTICFERLQHAKAHDYKNNSAQIASLVFYFAYSAAYNIGNNSLTYTYLVELFPYAQRTMGIGIEQLFGKLAGFFSVYVNPIALDAIEWKYLAIYCGWITFEFLFVYFMYPETYNRTLEELAFLFEDKALADEAVKAVEKQIHEAHHDGPTTEHGKVESTTTERAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7P7</accession>
    <name>A0A9W8Y7P7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable glucan endo-1,3-beta-glucosidase eglC</fullName>
            <ecNumber evidence="5">3.2.1.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Endo-1,3-beta-glucanase eglC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Laminarinase eglC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">N0V83_005611</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="25">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Glucanases play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. This enzyme may be involved in beta-glucan degradation and also function biosynthetically as a transglycosylase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <topology evidence="3">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 22">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7P7"/>
    <dbReference type="OrthoDB" id="77201at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000428">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050732">
        <property type="entry name" value="Beta-glucan_modifiers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018620">
        <property type="entry name" value="Ubiquitin3-bd_protein_But2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631:SF13">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE EGLC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09792">
        <property type="entry name" value="But2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0134">Cell wall</keyword>
    <keyword evidence="17" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0964">Secreted</keyword>
    <keyword evidence="11 23" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040832853" description="Probable glucan endo-1,3-beta-glucosidase eglC" evidence="23">
        <location>
            <begin position="20"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin 3 binding protein But2 C-terminal" evidence="24">
        <location>
            <begin position="322"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00025152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00032134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00032906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF09792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAJ4369847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="462" mass="49280" checksum="2174053646720E9A" modified="2023-11-08" version="1">MRVSSSVLAATGLLSTANAAIKGFNYGANFNNDQAKTLVDFEYEFNAAKQLAGTDGWTSARLYTMIQHGTQNTVIEAIQAAINTNTTLLLGMWASAGQSVIDNEIAALKAAVSQYGTAFTDLVVGISVGSEDLYRITPTGIENKAGAGAQPNDLVSYVQQTRNAISGTSLQGKPIGHVDTWTVYVNASNNAVISALDFVGMDAYPYFQTTMANSIGSASTLFFDAYHATVNAAQGKPVWVTETGWPVSGETLNQGVPSSDNARIYWEDVTCQLVKDNVNLYYYTLQDVQYGNPVPSFGIKPAGDLMAVSPLFDLSCPASAKPADPTKIIGNGYNAQLSPTISTIFVYDVNPIHQGKICTLIFQLPPAFQYPEFAPARISALGGISVSRLNNQVSAEVSVKDVGASNPVGIVPVIQFGQQYNVASGPCEAGQRVGYQVDSLYGLSMDWFQMTNPPLGLFMLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7X3</accession>
    <name>A0A9W8Y7X3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">60S ribosomal subunit assembly/export protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC1</name>
        <name evidence="11" type="ORF">N0V83_005454</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for efficient assembly and nuclear export of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the 66S pre-ribosomal particle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LOC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7X3"/>
    <dbReference type="OrthoDB" id="1743802at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037650">
        <property type="entry name" value="Loc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28028">
        <property type="entry name" value="60S RIBOSOMAL SUBUNIT ASSEMBLY/EXPORT PROTEIN LOC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28028:SF1">
        <property type="entry name" value="60S RIBOSOMAL SUBUNIT ASSEMBLY_EXPORT PROTEIN LOC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="139"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="56"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="173"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="186"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4369692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="197" mass="21718" checksum="76A3FAB59B4CD32C" modified="2023-11-08" version="1">MAPTKPSTKGKSAKGGKSSGSKSSRPKPTGKEPEGISKRRQKSMSLAKPGGAQKTKSIKSKDGKKKRRVYTEKELDIPALNGIIPAGIAKPKGVKKGKKFVDDPASMMAIMAVVNADKEGRLESKIAKARQLEEIREAKRKEAEVRSDDKKAKFEERKQDLKKKRRRHSAPSKGDDAENEPPEKKGKFKPRKRVSFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y854</accession>
    <name>A0A9W8Y854_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006736</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y854"/>
    <dbReference type="OrthoDB" id="2414723at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18316">
        <property type="entry name" value="BTB_POZ_KCTD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045068">
        <property type="entry name" value="BACURD1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145:SF8">
        <property type="entry name" value="RE57120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="33"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="264" mass="29876" checksum="E69477397FB3B521" modified="2023-11-08" version="1">MYSASIPPQSTSATPQPTPTPNMPQPAELPEGDLVTLNVGGLKFETRKSTLKFCDYFANMFSGRWTLELLPDGSVPIDADPEIFSILLAYIRRPTVYPLLWTKEQGFDYATYNRLLAEAKFFGLEALRSWIHKQKYLSAIKTSYKIYVSPACTGTWFSSLPADPPVIGFNNHAGYYKPISNIEVLQSFDLGVPGRGRCIGSKSYKDDPYNDAETFSMEYKPRQRGTHTEIRRPQYERLERSIASLWRSVEFHPEALMAGHGEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y864</accession>
    <name>A0A9W8Y864_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">rRNA-processing protein fcf1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">FCF1</name>
        <name evidence="7" type="ORF">N0V83_004923</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the UTP23/FCF1 family. FCF1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y864"/>
    <dbReference type="OrthoDB" id="76105at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09864">
        <property type="entry name" value="PIN_Fcf1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000019">
        <property type="entry name" value="U3 small nucleolar RNA-associated protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006984">
        <property type="entry name" value="Fcf1/UTP23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037503">
        <property type="entry name" value="Fcf1_PIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04900">
        <property type="entry name" value="Fcf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="PIN" evidence="6">
        <location>
            <begin position="67"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4370405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="196" mass="22527" checksum="D13F090CB681AB22" modified="2023-11-08" version="1">MGVQKRTRKFGQMKRLIGQRDARLKKNQMAGELEAKKKEEENKAKREIPQVSSALFFQANTALGPPYSVLVDTNFLSHTVHAKLELDKALMDLLYAKATPIITTCVMAELEKLGPKYRIALRIARDERWERLKCDHKGTYADDCIVERVMKHRIYLVATNDRDLKRRIRKIPGVPIVSVAKGKYVIERLPDVPDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8I7</accession>
    <name>A0A9W8Y8I7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_005723</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8I7"/>
    <dbReference type="OrthoDB" id="407832at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF2">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="29"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="81"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4369959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="491" mass="55426" checksum="994C38CE4025C8E2" modified="2023-11-08" version="1">MPCNRCRTRHYRFQFLDESSEIRSQYTADIDSPFSSPPIGPDLSPAPSSSRSGTGHHESPVTTLITPTEGFTVLSLLNSESPVQSQQPAPPVSHSHQASTIPFNDNQASTFVYQQPPGAPILWPLEHEQEATLLQHYIENVALFFDMIDHECHFGVHVVQRAKQNSTLMNAILALSARHLSRTTDFDPYIADAYYQRCFDTLIPALNDNVAIKEEPLLAATIILRLLEEMNISVIGSDPQGHLFGTQAIIRAAEQSYAATTGPSFRQAIYWAAFRQELWISLMTQRAFQLHIFPADRSLEPANDSIWATRTIAHLGDVSNFVFGEGRHGIAKYSQLMNENKSWRSCRPDSFDPFFFRQDRDGSGRNFPDIRFHEKSHVMGTQYNTLAHMLLIVHDPTIPQLGPAHKQSRATVDQMVQSDVRTLCGVSQSNAKVYPSKFVACFAIALVGDRFTMREDQERLRDLWYACERAHGFPPTTAISHLEESWGWHND</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8L3</accession>
    <name>A0A9W8Y8L3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EngB-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_004455</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8L3"/>
    <dbReference type="OrthoDB" id="391988at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01876">
        <property type="entry name" value="YihA_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052279">
        <property type="entry name" value="EngB_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030393">
        <property type="entry name" value="G_ENGB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46498">
        <property type="entry name" value="GTP-BINDING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46498:SF1">
        <property type="entry name" value="GTP-BINDING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51706">
        <property type="entry name" value="G_ENGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EngB-type G" evidence="6">
        <location>
            <begin position="109"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4371238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="339" mass="36983" checksum="9AF91A212671EF8D" modified="2023-11-08" version="1">MQRRDIRRSLRRARSERAAKDGGDLLPSTPSAPLGSRPEPKPAPIQASVSSNTSYKLPDGSLEDFNWYWESIAPTIAQKVHANHFFSNNGKNAKLLRSVAQFRLFPESDVPEVAFVGRSNVGKSSLLNAVVNADIKSLLARTSSTPGFTKTMNLYGVGPGNGVTIKKQPDGRDKIVGRSGLTIVDMPGYGEGSLAQWGVEIMKYIQSRKQLRRVFVLLDAEHGIKDKDRSLLASLRLAGVSHQVILSKVDKLYIPKTKEVKRHDGKKLSKLKSKGSPEALRKEMEKLKSEVQPPVGGGALGEILACSAETLVDGKRLGIDHLRYAILKAVGLEANVSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8W9</accession>
    <name>A0A9W8Y8W9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Peroxin-10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">PEX10</name>
        <name evidence="21" type="ORF">N0V83_005287</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8W9"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016562">
        <property type="term" value="P:protein import into peroxisome matrix, receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16527">
        <property type="entry name" value="RING-HC_PEX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000395">
        <property type="entry name" value="Putative Peroxisome biosynthesis protein (Peroxin-10)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025654">
        <property type="entry name" value="PEX2/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23350">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23350:SF0">
        <property type="entry name" value="PEROXISOME BIOGENESIS FACTOR 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="331"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ4370765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="382" mass="42286" checksum="601F6ED892E16F96" modified="2023-11-08" version="1">MASSKPIPFTYPFATSPDIIRSHQKDAYFSGVLLDQLSTLLRKLYGARFAHTYVSETRVIGELLYLGLTTLIGNRTLGEEYTDIVQVESETGRLPALGRRAGYILGYILSPYLLGRILPKLRRRVRAKLEANLRAYGRQMARAQHHAKESGSNKTISKPLGMRIQNYILQNLDTITSPSPIYALSLATFYFSGSYYHLSKRIWGLRYIFTRHVPESDNRAGYEVLGVLLVLQMAVQGYLHLHNTVTTSTAAVGGGHPTGTSALVGGGAEVSLDPNAYSANNALLFDAAATQHTTPPSDVQKWTNTPAADKPRYELGDEDTMGWISGGNRKCTLCLEEMKDPSVTTCGHVFCWTCISDWAREKPECPLCRQSCLVQHVLPLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y993</accession>
    <name>A0A9W8Y993_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Deacetylase sirtuin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_004607</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y993"/>
    <dbReference type="OrthoDB" id="424302at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF10">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="5">
        <location>
            <begin position="9"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4371390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="323" mass="35438" checksum="A863ED7025D43FEF" modified="2023-11-08" version="1">MASSATRPSVVSPKDLESFQQHLTQSTRILALLGAGLSASSGLPTFRGAGGLWRTHDATSLATPEAFERDPGLVWQFYSYRRHMALKAKPNPAHYALAELARKKRRLHYTESERLSPRAQHPASQLKLLHGSLFDVKCSDFFCNYVEKNNYTDPIVPALAIPTDESDPTSTSALAARGIQPNELDISDEGVALPELDYSHLPKCPKCKRGLLRPGVVWFGEQLPSSVLDDIDDYLKTEHKIDLIMVIGTSARVYPAAGYVDMARMRGAKVAIINMDPNDIPAGGLYDGDWFFQGDAAQIVPEILRSVIGEIKMEEVSDAAEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y998</accession>
    <name>A0A9W8Y998_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004479</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y998"/>
    <dbReference type="OrthoDB" id="3687991at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="398" mass="44416" checksum="329F59D3BC20D7AF" modified="2023-11-08" version="1">MSSTSMPSKRKQADVEPTTNSLPPNPAKKVKLSLPEPSKPTLKMTEKIKLRSHSLPRARPEAINKVKPSSQDSSKATSCQTSEKVKLRSQDTPKATTLKKRKTSDDVSDPVPTYHNPAKKAKLSSVSTKKVTSKDVTKSATTPLPTTFSTAKSTLIPRGNRAKATLGQQDDSLLRVLCDDSKTGEAKELHFRKIPHSMVDWNKPQHIKDINSWRNQIYGRAGVKSKQVTMWHEEEELWFELYFHLSIAESRSRGMLLPKTKEVLDAFNKTFVGCVLTDKEGNELEARAERQLNAFASKFNRMCPELKARLNQSVFGKSGDIFLPKITNGMLEEYRQMRSEMAAQGITEESEYSDNIEVWQTFLSHLPSSSDGEAQEDTLLSEEDSEAAATLVSMANAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA38</accession>
    <name>A0A9W8YA38_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_004070</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA38"/>
    <dbReference type="OrthoDB" id="433512at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="340"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="395"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="492"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="520"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="108"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="638"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="656"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4372296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="669" mass="75553" checksum="3648DBA1F3E54A7B" modified="2023-11-08" version="1">MAASSAPSLRDYGVAAPHGPNMTGEHTATTDIDIRHDMSENEASVFHALLHPDDMYDANGTYWADLPFGQRVKFVRNVDAQEYKAEAKFFWDMFKKDPLAPIGHYFRTCVIPGAGLGLEGYVLFSIGNVRPLLQAAFPTCWKTFSICNKTWTQAVDYLEILGIIVGQILVGILGDWLGRRWGLIQDAIIMFLGLIMLTAAWGVTQNGWVICYAWALFFYGVGVGGEYPMTATAGMENAVGSGKVSTKDDRLHRGRKVTSAFLMQGWGQFLNQIILIVLLLIFHHGSKPPYSKRMAQWTYRVSFAIPAVGTLWLVYYRYFKMRSASKQLMLSKKKSKVTGYDVTSLKLTISYFGPRLLATAGAWFANDVFFYGNKLFQDEFIKVITPGNTSVMVGWLYNLVNVGVSLCGYYLASLMIDNKMYGRKKMMIVGFFADFILFIVPAFAYNFFTSPEHVHAFQAMYFLSSFFNQFGPNSVTFLVAAEVFPTPVRATAHGFSAAVGKSGALLASVLYNYIDIQTKFYFVPWFGLAGALLTILFLPDTTGLDLKEQERRWAFIRAGKEHEYHGIAIHWKHLSWWERFRGQHKGYDAAKDYEQKINELRADWYERQQSKFSEKHGWEGAEPDEDDHVDVHNYFKRTTAGSSLGGEPLMAPNSESDSRNSDAIMKEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA44</accession>
    <name>A0A9W8YA44_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Chromatin remodeling complex Adenosinetriphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ISW2</name>
        <name evidence="16" type="ORF">N0V83_004636</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA44"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17997">
        <property type="entry name" value="DEXHc_SMARCA1_SMARCA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000082">
        <property type="entry name" value="ISWI chromatin remodeling complex ATPase ISW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000002">
        <property type="entry name" value="ISWI chromatin-remodeling complex ATPase CHR11 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000234">
        <property type="entry name" value="ISWI chromatin-remodeling complex ATPase ISW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.30:FF:000003">
        <property type="entry name" value="ISWI chromatin-remodeling complex ATPase ISW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.30">
        <property type="entry name" value="ISWI, HAND domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044754">
        <property type="entry name" value="Isw1/2_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015194">
        <property type="entry name" value="ISWI_HAND-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036306">
        <property type="entry name" value="ISWI_HAND-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015195">
        <property type="entry name" value="SLIDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF49">
        <property type="entry name" value="SWI_SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY A MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09110">
        <property type="entry name" value="HAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09111">
        <property type="entry name" value="SLIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101224">
        <property type="entry name" value="HAND domain of the nucleosome remodeling ATPase ISWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="208"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="504"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="15">
        <location>
            <begin position="869"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="44"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="138"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1046"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="45"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="156"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1059"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1090"/>
            <end position="1112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4371419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1120" mass="128222" checksum="7E225E93E55C1869" modified="2023-11-08" version="1">MARKSNVDSDGPSSGDASAHSDVEMHDRVDRMSGFKKFKYFQDTPDYTDSDTNPNTTASSIAGDHAPLDGRKKRAEVLNMRKSIYGKKHDRLGASKEDDTIRRFRYLLGLTDLFRHFIDTNPNPHIKEILAEIDRQDAEEEKKSKASKVRKGGAAAERRRKTEQEEDAELVRNEKHGGHNETVFRESPGFIQGGTMRDYQVAGLNWLISLHENGISGILADEMGLGKTLQTISFIGYLRYIAGITGPHLVAVPKSTLDNWKREFSKWCPEVNVLVLQGNKDDRAELIKERLVDEKFDVCITSYEMILREKTHLKKFAWEYIIIDEAHRIKNEESSLAQMVRVFNSRNRLLITGTPLQNNLHELWALLNFLLPDVFGDSAAFDDWFSQQNADSDTVVQQLHKVLRPFLLRRVKADVEKSLLPKKEINLYVGMSDMQVQWYKKILEKDIDAVNGAAGTKESKTRLLNIVMQLRKCCNHPYLFEGAEPGPPYTTDEHLVTNAAKMVMLDKLLKRMKAQGSRVLIFSQMSRVLDIMEDYSVMRGYQYCRIDGSTAHEDRIQAIDDYNKEGSEKFLFLLTTRAGGLGINLTTADIVVLFDSDWNPQADLQAMDRAHRIGQTKQVYVFRFVTESAIEEKVLERAAQKLRLDQLVIQQGRTQQPVKNAASKDELLTMIQHGAEKVFNSKGPVGPAAEGDLGDDDFDAVMKRGEEMTKKLNKRYETLGLDDLQKFTSDSTYEWNGETFQPRKKEIGLAWINPSKRERKEQNYGIDSYYRKTLVTGGRTENKQPRIPRAPKQIHIHDYQFFPDRLAELQDKETAWYRKENNLKAPLPEGPDEDIEAREADQELAQKEIDNAEPLTEEEKAEKERLIEKGFPEWNKRDFQQFLNGSAKYGRTNYDGIAEEVDGKDADEIAEYAKVFWKKYKTLDNWQKHLGVIEEGELRVRQSEEKKRLIAKKIGMYRMPLQQMVIKYTVSTTNKKVYTEEEDKFLVVMLHKHGVEGDLIYEKIRDEIRESPLFRFDWFFLSRTPQEIGRRCTTLISAIVRELGEGDMKNGKGGKRAYEEEDTEEEEEEPVKKKAKNGVKNKQLDAVKGKASPASTSTSRASSVASNGSVAKSKGKGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBK4</accession>
    <name>A0A9W8YBK4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBK4"/>
    <dbReference type="OrthoDB" id="2305901at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="644"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="655"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="673" mass="75254" checksum="96C492D12A76F11F" modified="2023-11-08" version="1">MAATKAMADRHIISSILHQLSDMKYKDRRQPGPDQPVEFMEFRPTLVPSILVNKMWAEEGTSILWKGYPHLPALKGMDINRRQWYANKVERLFVLGPSLDSAEDPAYLEGLMWPNLKYLELEVDWKTHGKSFEAMLHGGLEHLEFSGSQSGDSKFITDVILPALVAPCKNLRSVHFGPDASDPEDPVSAYDLSNILDSVPNIKDVRIMNTTFLDKDVLFGRLSQRPGLRALEIDLEPGLQLLPLLSGPNPLPSPFSSLKHLHVMCYPEIALALPIHIRRIEVLYLDVARIPDQPFQDLDTSVLDDILHALSKCPELQSLRVNIGPLAVDFPSFSNYPSLSGPALITLATGCSKLREVNLLASEPATIDGSLISSIQFEEFCRKAPHLEELSLKFHPGTVIALEPIVLQSLGRHCARLGVLRLKSALHLPDLPKVNVASQDEQAHATSATLQERSEVLDPPAITINGHIYGDYPMPRSAPDRPLFPRLTHLALARPQSILSIANDTYTISSTSQSSSIVDPTLEEDLVRTWAQSLHANFPHLEILEAWGDWTGQDNESLNYFLPLEEILASTWEFLSGVEQDLWEDGADLEALGDDVEEWDEEFEEDISFDSHGSGDDWEKASLINEFPVEDVAVDREYLGVYEEEPEGTITPGRTIDKEAKNYFEHTRDQHKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBM7</accession>
    <name>A0A9W8YBM7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_005285</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBM7"/>
    <dbReference type="OrthoDB" id="2019572at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051836">
        <property type="entry name" value="Kremen_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269">
        <property type="entry name" value="KREMEN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269:SF16">
        <property type="entry name" value="PROTEIN SLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="WSC" evidence="8">
        <location>
            <begin position="253"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4370763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="475" mass="52799" checksum="60C70E919E1E5B87" modified="2023-11-08" version="1">MRYTFFQLARDTGAQAILKAAPHFRALFGTSDHYSTDQTPLLCPQSDSTIESVYPPQWDPLNQIEGSDALLRLRKEQGWRWRQEHTEREAYVQEQRRYSTMSIQTSAQQVEQRYSLGQTHRVPSWEQQGGVLQHNVPEQDIQGVVTSAQLPKSPESTLFTNSGTDTHATEESASQPIRIRLGERENDLNGYHELRTESSSSSSLRRHDAVRYRRNPLFYRDMPSSPYLADSIDESHGGGGQPGTSVKITAFAPNTYEGCYANLQTTTGLSLLSGPSVTASDMTHQACLAFCNSQSGAPYKYFGIQGGTTCRCSNTQTLSSSMSEDACSTRAGGNSTQTGGGPDELAVWQNAAYTAQSLFLSPHPQDNIDDTSSQHNYRRRNTTMGSDFTFALASITYDFGTRLQTGADHVPTSLREDNEELDSWPLLKIDDTVRTIPHKKETAVETKISGGCHGGSTSYFARFHEDENKESKKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCD7</accession>
    <name>A0A9W8YCD7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbohydrate kinase PfkB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_002533</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCD7"/>
    <dbReference type="OrthoDB" id="204058at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052562">
        <property type="entry name" value="Ketohexokinase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42774:SF3">
        <property type="entry name" value="KETOHEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42774">
        <property type="entry name" value="PHOSPHOTRANSFERASE SYSTEM TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="4">
        <location>
            <begin position="307"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4373794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="362" mass="39323" checksum="5CC05A7573523A2C" modified="2023-11-08" version="1">MSDNNQTTNHIICVGAVYVDTILTVPHFPIEDEKLRAKKLARRRGGNTANTLEVLTQLLEHDPTTTPSSSNGISTSTTELHLLSVLPDKASNAAGFVRDSLPGVDVDEGSIFRGGHSDAASSYIIQSQQSLSRTIVSINELPEMTVDEFIASVNNLVSKQQSQHQAWKKVWFHFEGRIPDITLQCVTYLRRQFPEFKISVECEKPERTQMREVAQHADVVFYSKLWAEKNGFATARAFLEAQRGETRDGALLCCTWGSGGAVVVQKKGGGDGEGGEEEVWADVEAWKGDDGGGGRGGGRGEGDGKGVVDTIGAGDTFIAGMLFGIVRHGRDWSLGQKLEFANQVAGRKVLQEGFGGLGRQMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCL0</accession>
    <name>A0A9W8YCL0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_002863</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the paxM FAD-dependent monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCL0"/>
    <dbReference type="OrthoDB" id="2431938at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050562">
        <property type="entry name" value="FAD_mOase_fung"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47356:SF2">
        <property type="entry name" value="FAD-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47356">
        <property type="entry name" value="FAD-DEPENDENT MONOOXYGENASE ASQG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="390"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="453"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="6">
        <location>
            <begin position="13"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4374122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="479" mass="53326" checksum="FC6FF3F81903DA84" modified="2023-11-08" version="1">MAHTGSTSAKPFRVIVVGGGIVGLSLSHALQLANIDHVVLEKHDKIVSVRGAALIIWPNVSRIFDQFGFLTKIHETTTPVSFECRRWPDGTLHSRAFVLSKLSELFELPSILFDRQALVTHLYENLPDKSKIHTSRKVDHIEHTEDSVRVFLSDGTIEEGDMVIGADGVHSIVKQQMWNYASKAEPGAIPESDKTAVFSEFGGLFGVSEMKDSFGMGPAESNIVFGHGYTNLLFSQPGKVYWAVIFKDERSQPPKRRKNDEEATKAEAARFADIVFTPQIKFKDLWEKRSRSGLLNIEEGIMDKWNAGRMVLVGDSAHKMTADLGIGANIAIEAAVTLCNILHRELKSNPNRHPSQSELAAMFAEYQKDRFDRAKAFVELSGKATRMHSYQTLFGRFFVGYIAPWLMKMQVMQFAKSFAEAPKLEYAPIRTVNEKAEGWKLAKKSESKASAGWLTYVILTSTVGVTMAYIATNGLPALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCT7</accession>
    <name>A0A9W8YCT7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Bifunctional hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase fox2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">FOX2_2</name>
        <name evidence="5" type="ORF">N0V83_004870</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCT7"/>
    <dbReference type="OrthoDB" id="3592703at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051687">
        <property type="entry name" value="Peroxisomal_Beta-Oxidation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024">
        <property type="entry name" value="DEHYDROGENASES, SHORT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024:SF2">
        <property type="entry name" value="SCP2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4371650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="268" mass="28974" checksum="79BC7C006CB1047D" modified="2023-11-08" version="1">MTAVANYDSVENGEKIIETAIANFGRIDVLVNNAGILRDITFKNMKDQDWDSVINVHLQGAYKCTKAAWPHFKRQKYGRIINTTSAAGLFGSFGQCNYSAAKLALVGFTQTIAKEGVKYNVLSNAIAPIAASRMTETIWAPEMLANIAPGWVVPLVAVLVHSSSSETGSIFEVGGGHIAKLRWERAKGLVLKPDDTYTPGAILQEWSKISDFSEPEHPNGIADMMEKLKEAMTLDSNDTSVETLDFTGRVALVTGGGAGYVLKDSSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCU1</accession>
    <name>A0A9W8YCU1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF1740-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_003709</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NRDE2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCU1"/>
    <dbReference type="OrthoDB" id="297219at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902369">
        <property type="term" value="P:negative regulation of RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031048">
        <property type="term" value="P:regulatory ncRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013633">
        <property type="entry name" value="NRDE-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13471:SF0">
        <property type="entry name" value="NUCLEAR EXOSOME REGULATOR NRDE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13471">
        <property type="entry name" value="TETRATRICOPEPTIDE-LIKE HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08424">
        <property type="entry name" value="NRDE-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="167"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4373414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1022" mass="115753" checksum="FB64C9BBCCA79D1E" modified="2023-11-08" version="1">MASNVPKFASFRPKPKAAPEPPKELRNPSSRERPLQEKTRTKATSSLQSERPANSRDTLTSRAYFSDRRGDPDVRKYGTLNRYDIPAYRRYGHGVVLGLAPDQRIDRDQTTDASIQVTPVGRPRQQRLLLDRHLNKSTARSLRLLKAGKADIHPSMDYVALPGGGTRKQEDSDHEGEGLSHVDYRNIESKRRSAEPNDLDTRYESDEGTIKIDAEVTRKNSELVRQTREHPETLQNWLAFIAHQDSMLKLDAPSSELTASEKTQLADVRISIYEEALKKIGKDPHSQMKLYEGLLSEARRTWSETKLASKWNEVLAKHPHSSELWFMYLDFVQSSFATFKYEDCRSAFLKCLNALRASPGTIAFVDVLRVQLRLTRMMQESGYQELALAIWQAILEIHFLQNVPDAPFGPEGPVSAFAEFWESEAPRIGEEGATGWMIPNTNDISPSGSVSLYTSNPSDTVFEGFRKREMDAIDKLRYPGRTSDDLGEDDAFHTIFFSDVADYIIFPLETPFMFVMQAFLCFCGLPPLPEDISQQHKWWSDPFLQRQLNSTSPSQEQPSHFMQNLNRFSKCPLQSFSMTSELLFEQDFSLDAIRLSPDFIRRALKLLVTDASYGETIGEYLLAFESRHFPADAVKTAKRLLKARPSSLRLYNAYGVLESRRGNTVKADQVFSLALSMQKGDVTFSTPSSLQLFSNWTWESLHRGEQTEALWRLGSPLGKVSGRNEQHERPGHGHLLATRKVLAETSERALLQQDYQSAVLATSLLALTAYLSSDCKPEPAIVAHQHLSSWFDSHKLSASPSAELHAQQIARFLIHHATHAPIVKPALIRETLEPFIYLFPNNTVLLACYAANEARSSIDDRVRSIMHQTARQSSDSVGVVGWAFAIHFETLKGEIAGSTSHSIRALYNRATEGIGAHCPALWLANLRFELQIAFQEQKKCAERKLAKSKKKGSGEARASAAGARVKERYYEGLKCLPWCKDFIMLAFTDAREFLTEEELWRLYNIMMEKELRLYVELDEPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCU2</accession>
    <name>A0A9W8YCU2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Translation factor guf1 mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">GUF1</name>
        <name evidence="11" type="ORF">N0V83_003242</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.n1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the GTP-binding elongation factor family. LepA subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCU2"/>
    <dbReference type="OrthoDB" id="1074at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097177">
        <property type="term" value="F:mitochondrial ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03699">
        <property type="entry name" value="EF4_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01890">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03709">
        <property type="entry name" value="lepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000078">
        <property type="entry name" value="Elongation factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000015">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000007">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2570:FF:000001">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000004">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2570">
        <property type="entry name" value="Elongation factor 4, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00071">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006297">
        <property type="entry name" value="EF-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038363">
        <property type="entry name" value="LepA_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013842">
        <property type="entry name" value="LepA_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035654">
        <property type="entry name" value="LepA_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01393">
        <property type="entry name" value="lepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512:SF7">
        <property type="entry name" value="TRANSLATION FACTOR GUF1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512">
        <property type="entry name" value="TRANSLATION FACTOR GUF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06421">
        <property type="entry name" value="LepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="62"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="71"/>
            <end position="78"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="135"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="189"/>
            <end position="192"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4372951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="660" mass="73903" checksum="566F839D76B4006C" modified="2023-11-08" version="1">MRSCVRTASSLLQGWRTHSAPPNGFDRARRSLERLAPLARSYAQAFRPQSQLEKRIAEIPIERYRNFCIVAHVDHGKSTLSDRLLELTGTIEPGGNKQILDKLDVERERGITVKAQTCTMIYKHDGQDYLLHLVDTPGHVDFRAEVSRSYASCGGALLLVDASQGVQAQTVANFYLAFSQGLTLVPVLNKVDLPHADPPRVLEQMKETFELDPDAAVLVSAKTGLNVAALLPTVVDKIPAPVGHLSKPLRMLLVDSWYDVYKGVILLVRIFDGQVRPGDTIRSFATGLKYIVGEVGIMYPDQTPQTVLKAGQVGYIHFNPGMKRSQEAKVGDTYTTLGSEKLVEPYPGFEEPKSMVFVAAFPTDQDNHEHLEDSIEQIILNDRSVTLQKESSDALGAGWRLGFLGTLHCSVFEDRLRQEHGANIIITPPSVPFKVMWRDGTESIITNPNEFPDQDQAHFKVQEVHEPYVSATVTLPDEYLGEVIKLCESNRGEQKELTFFTATQVILKYDLPLSHLVDDFFGKLKGATKGYASLDYEDAGFRKSSIVKLNLLVNGQPVDAVSRVLHTSQVERVGRQWVEKFKEHVERQMFEVIIQAAAGRRIIARATIKPYRKDVLAKLHASDLSRRRKLLEKQKEGRKKLRAVGSVVIEQEAFQKFLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCX1</accession>
    <name>A0A9W8YCX1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003287</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCX1"/>
    <dbReference type="OrthoDB" id="4096268at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051176">
        <property type="entry name" value="Cent_Immune-Sig_Mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 170 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715:SF37">
        <property type="entry name" value="LD47843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="48"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="592" mass="64101" checksum="47F80ACFC341DC7F" modified="2023-11-08" version="1">MTESEILLPSGMPRSSTNMPVFRIVLEDVHQYDIYASREFDLAMDSTFPIGRASKNATKKELMPAAYNAYIDSPVISREHAVLSANTSSGTPQVYISDKNSMHGTMVNGQPLTPNTPKQLSAGDVIQLGLDVNRNEGTYSNGDITLPEFLIANFYVPAEYFVARKYTFDYRLTQPFSRGFAVPDPESEEEELEPHGRRGSQSNPLILDDSEAGSVQSDDDLRDVTVVEEAAVFIDEPTAATGAPFVDEKDPAAVMRAAMIKQAESNLEEDECSVEYSSQSEDEASSEADIDIDSEAASVGSSEPEYNSSDREVPDTDEEAVDDVAYKKWSTDNLPTVQPRDLFNMDLTNGQAPELPSLGVFSSGHLNTTPFHGSMMPPMPSSLSASKQAPWEKPKFTSFGQQSDSQSWYSDEMPGLPQSYLGTNFGDRPSIFSPAPPPPGLESSRTQKNEIAWGGAMSSAPPADGMQTPPPLPASDISTNSPLLQPNRRTKVSIEEIVEEQPLTPTSVGGLKRKADLLEEEEEFVTEPVVVSEGVIDAAAQTATSIAQRPKKQPRSVLSKVLNKAAYPLLGAAGAVASFTLLSTLPDTFFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDD3</accession>
    <name>A0A9W8YDD3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDD3"/>
    <dbReference type="OrthoDB" id="5279008at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="19"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="474" mass="53359" checksum="A0A957E5BE2C8705" modified="2023-11-08" version="1">MADTFVNARTASPETTESHFRILDFPTELISNVCVHLEDDDLMNVRRACRALKAHSTWAFGKRFFQHLVAILHPTSLAILLEIVRHEVLSVFVREVTISGEIYGFTIAPKKIHSRPHLAIQASIKNSGLDILILLEAFKTLRNLASVRIDVDSFWRSCGIKCGRVYLYEGNARDIKPVYHPEVLYNRVYEVVFQALHSASVAEGVSLSVKICTGSYGDDSISFFNVQTPAWEKSFAMRLRDVEAHGTMDTVWLHRLICSATDIHSLSLHSEHNMFSFARTTGAVLQWPGLRRLHLHEIILKHKDFLPFLRGHSLYLEELHVDSVGFLDGNWVEPLEAIEGMPKLVHLSLMDLLERTHYENSSSSSYMFDMEGLGGALSFRNDPRGITSALACLRGDLQTEPMYCYPSPGSIYTDDLEAYVNLRKAEALSQGWLSWIDGEWVMASAPSSPPISSIEDVLSDVLEDVEDETETTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDM0</accession>
    <name>A0A9W8YDM0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SMC3_2</name>
        <name evidence="12" type="ORF">N0V83_001883</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMC family. SMC3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDM0"/>
    <dbReference type="OrthoDB" id="431497at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03272">
        <property type="entry name" value="ABC_SMC3_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000370">
        <property type="entry name" value="Structural maintenance of chromosomes 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000424">
        <property type="entry name" value="Structural maintenance of chromosomes 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041741">
        <property type="entry name" value="SMC3_ABC_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="11">
        <location>
            <begin position="523"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="646"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="690"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1055"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="194"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="282"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="352"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="414"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="851"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="690"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="708"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1062"/>
            <end position="1073"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4374806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1215" mass="138073" checksum="BDFD2F0DD1712977" modified="2023-11-08" version="1">MGYIKQITIQGFKSYKDQMQIEPFSPNCNVIVGRNGSGKSNFFAAVRFVLGDDYHFLSREERQALLHEGSGSAVMSAYVEVCFDNTEDRFQTGKPEFFLRRTIGAKKDEYSVNRKNATKAEVQQILESAGFSRSNPYYIVPQGRVTALTNMKDSERLRLLKEISGSNVYETRRADSLKLLADTDSKCSNIDSVVASINERLDELEGEKAELEAWSKNDKERRSLMYTLKSREEADLEGLIENIDLLENQGREMREDNEAAFMQNEADISQIETDIGKRRGDLDILQEDRVQSEQDRKSATLEKAKIELELKALQDNQSVAQQMKRSRDTQMKSLQQQIRTREAQLKQLLPQYNAKKEEEEAVRSQLMEAEGQRKRLEEKQGRTAFYTNKRQRDDALRAQIDESNGDLSRRKAVLMQTNEEVVQLQSDIERLENEVAELRSTIESEGDASVNLAANIEKAKDAARAIRDEQTHLYREENRVNTQLTGARDDLRKAENTFSRLLDHGTSRGLESLRRYQQEGSLEGVHGTIADLLQVPETYRSVTENAAEGALFNVVVDNDNVSSKLIDRLIKDKGGRLTFIPLNRVQAPNMQLPTKGDMQPLLPKLSYDDRFDAAFKHVFAKIVVCPNLDACKSNAKQYNVRAYTPDGDNASRKGQYRGGFHDPSKSKINAYQKVIAARTQHEELQQRKHDITRELEQKRQQATAASSEIRRREHEKNKGENSYGPMREELRVKHKALQDARDSLTRKQRTASDLQSAINQLGAQQSDWEAEIASKFEKALSRDEEQMLVTLTSTVQDLRRQSARIQTERTALETQKVEAELDLNENLQPELDNLQAQQSGAGGSTSQSTRLGEYERALEAADRAIADLDQQIQETDAQIEDIRAQLLELEAARSEKEASNRQLARAMEKHELAMSKKDSDRSRHTDRLAEVKRDIRDLGTLPEDVHRKYARWDTTKVTKELTKANQALRNFAHVNKKAFEQYENFTRQRRTLTDRRAELDTSRKSIEMLIDTLDQRKDEAIARTFRQVAQAFKEVFQQLVPIGQGRLIINRKSDRDVRGNVSDDEDDESEEETQAQKGNKVAEYIGVSIAVSFNSKHDEQQKIGQLSGGQKSLCALALIFAIQKCDPAPFYLFDEIDANLDAQYRTAVAQMLQKLSGQGGKAGDGGGQFICTTFRPEMVLVADRCYGVSYSNKTSSIDVVEREAALDFVDGMQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDR4</accession>
    <name>A0A9W8YDR4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">COG4</name>
        <name evidence="11" type="ORF">N0V83_002033</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDR4"/>
    <dbReference type="OrthoDB" id="47059at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048682">
        <property type="entry name" value="COG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048684">
        <property type="entry name" value="COG4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013167">
        <property type="entry name" value="COG4_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048680">
        <property type="entry name" value="COG4_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24016">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24016:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20662">
        <property type="entry name" value="COG4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08318">
        <property type="entry name" value="COG4_m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20663">
        <property type="entry name" value="COG4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00762">
        <property type="entry name" value="Cog4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="COG4 transport protein middle alpha-helical bundle" evidence="10">
        <location>
            <begin position="173"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="333"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4374954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="783" mass="86448" checksum="006A2D97BE01D508" modified="2023-11-08" version="1">MILENGTHEPRDIFAATSVAEIHAALHQLHQHEATVTQRLNALIASQKDLSRELGRLDLLRAHLGTQAVNTRAISNGMLSDAASTANRISTAVKRLDQEQSNVKATLDVVEQVAELKACVLGVNGSMGAPQDWETAAGYLSRASKIPDDVVNGSFAEEIVPTAEVPDPPRVTLDAAAESLCGLFLREFEKATKEGDGSKVTRFFKLFPLIGRTDVGLDAYGRYVCQGVASRARTNFNSAAPAQRNEGFFYGNTLTKLFEHIAQIVDGHEPLVERHYGPGMMQKVIERLQIEADVQGGIVLDTWHEERNIDRKLTDIKSYAFSFLVQSFLPAQKSATGTPRSNSPANGVVRTSEDEGVNMKEVDGLLGESALMLGRYALYSRFISDKCAPTEPEDRIDHGLVMPQFLATSNLHKKITSHLIEPVNVMTTFFFRRSVEKAFQLDESPSDLTLNPTKPLGSNPPFITSAVDDVMYIVNQVLQRTLATSQRAIVSSVVPTVSQILGSDFIGMIQRKMRDESYPKPVIQGGLPPEDKVLSFLVLINNLDIGNDYIKRIVQQQLGTQSQGDQEAIKSPLHDLFPFGHDATFVENTLKAMEKSFAAKSGDLLNDGITVLFANVLKPRIRPMLAEAFRDVDYAPEDDDDLNEDVEEDNVDLVKARFDRGWGVVIRPLKRILTTANFDRLLTFALNSLASALEKRIRGYYGRVNELGAVRLERDISGIITAAVAGGKYGLRDAFTKCTQMTLIMNMEDDEWDEVANDTSGESGIPWVLDAEERKRVRAIVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDW8</accession>
    <name>A0A9W8YDW8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDW8"/>
    <dbReference type="OrthoDB" id="3533623at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="465" mass="52294" checksum="0A15BF2DF57FEB16" modified="2023-11-08" version="1">MASTHSRSASDEANVSSMSYILEHVLQYPGSYDVPLRTMYELNRKANSLPKSRAQTPTGRSGDASPVTGQFAWNSAETATMSFTSQLMKQLKSLPTRPSSLPPTFIVNFVSRCFHPSVGLVDWNQSLTALDYLNDLDSRRRRETSAAFARLNIHEDTYGTDMAFMAEKFPGIALWVRNIEGKNKKADQYYAIMWLGLRRWIMINVLSDEPFNKLDCLSLLNTLLPPMYDPKKLPPYLNQQSVQEERRRFFDLISRVQRNGPAILQTVIDENKAPGDVTGWTTVHTVVDKYLRVARNIIDDCLKTTGPETFDRYADERNKEKKHDSGVSFGSERRPSVGTSLHEKPAPEPLSTFTPAPKGLSTLERIAREFKRMRVKPRPEVEEITQVNQRADAAAAEFIPQSGENAGKKKLKKARSLASLKFNNGSSLSLASRKGSDTVPFGAAQMKKQRMMYEASVSKNGYSHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE18</accession>
    <name>A0A9W8YE18_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycoside hydrolase 131 catalytic N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002095</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE18"/>
    <dbReference type="OrthoDB" id="120072at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041524">
        <property type="entry name" value="GH131_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34612">
        <property type="entry name" value="GH131_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34612:SF6">
        <property type="entry name" value="GLYCOSIDE HYDROLASE 131 CATALYTIC N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18271">
        <property type="entry name" value="GH131_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040947230" description="Glycoside hydrolase 131 catalytic N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase 131 catalytic N-terminal" evidence="2">
        <location>
            <begin position="20"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="262" mass="28574" checksum="6FE863C2A5823A11" modified="2023-11-08" version="1">MFGKAAIANALVSAVSAGTILWDGRFNEFTSSADLDKWSWGNQVGPYQWYIHGSGATSKYVNLSPSFKNPADTGSKQGVKITIDSTAKWNSDMWRTELIPQTKAAINSGTVYYHFSIKRSATNVPSATNEHQIAFFESHFTELKYGWISGESGTSNTNLQWMVGGQSKWKVELKADEWHNVAYEINFSSNQVTFWHSTGSDPLVKTAGPFSGSTSSNGADWHLGVLRLPRQGNDGTGAEDWYFSGTYVESGSLTTAVASPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEF5</accession>
    <name>A0A9W8YEF5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Asl1-like glycosyl hydrolase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEF5"/>
    <dbReference type="OrthoDB" id="5959761at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071966">
        <property type="term" value="P:fungal-type cell wall polysaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053183">
        <property type="entry name" value="ASL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024655">
        <property type="entry name" value="Asl1_glyco_hydro_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34154">
        <property type="entry name" value="ALKALI-SENSITIVE LINKAGE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34154:SF3">
        <property type="entry name" value="ALKALI-SENSITIVE LINKAGE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11790">
        <property type="entry name" value="Glyco_hydro_cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Asl1-like glycosyl hydrolase catalytic" evidence="1">
        <location>
            <begin position="25"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="224" mass="24801" checksum="44B39F32280F8B3E" modified="2023-11-08" version="1">MKANSPLVVFNPDDVARGLELLNQANASNPPPAYMELLNEPDIPLSGRGPISPRAAAKAVQPILDYNKAHNVTTLLAPALSHPDGIFDADGWLTQFDGNCSGCITSMDTPIIATHQYNLDAEGVLKSLNKIHKRWPKHSIWVTELAPAVHAWNNCPMTVEEVGDWMETVVRGTAELDYVERIFWNSGEWRPKISNHDCIHSLTHDDGTATPLLKRYESLCKNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEI3</accession>
    <name>A0A9W8YEI3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002879</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEI3"/>
    <dbReference type="OrthoDB" id="10260961at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42103:SF2">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42103">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="411" mass="45562" checksum="856939449F331C16" modified="2023-11-08" version="1">MAQKPRHPPEPHYSFTIPSVHDDTTLDCRVYHPPALSGESDESGASWKKRGIVMAHPYAPMGGSYDDRVVGIVVEEFLKRGWIVGTFNFRGAHGSKGHTSWSGKPELDDYSSFAAFFLHYVCYLQPSPTPDTLFTPEQSPVSPQSHTLSDSTRARNEEPPNVVLGGYSYGSLILKHLPPVPTILQPFSNPTTGTAADEILLRAHNLAGQSNLEWTNLAKDQAREKRGKHGHEHNLSVTMGGEETSPEKRRRSREFRRSFEGNRSLDLKTRLRSLSHRRRPDEAPIMPPDAIHSVSSSSPITAAIRYLLISPLTPPISTLAAPTLGPKFWSRPKEGGQQDDIGKHPALAIYGDQDIFSSAKKTRAWAEQLKAGSGSQFRSVEVEGAGHFWVEVGVEEKLRVALREWEVGVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF91</accession>
    <name>A0A9W8YF91_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nephrocystin 3-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001198</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF91"/>
    <dbReference type="OrthoDB" id="416222at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF5">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="3">
        <location>
            <begin position="10"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="676"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="684"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4375920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="704" mass="80167" checksum="8B40150B35ED888A" modified="2023-11-08" version="1">MAKWSAHDVISDPAEALLTLKGNPGTGKSVLIKAATQKAESPNLIVLHHYFDRAKDSKSSTFKSALDKFCTSLLYQLLTQLEPKPWSFLYEWRELLRFNPGMLPWNLTQLQDAIREIIVGHEKRGNVGVQIYIDALDECYNDISDVHPTQPLEVLQWISKLLKSAADANVDVRVCLSRQHLPAYDGLEPKSQTIIVEDYNRSAIETFVKTELQRVEDIGLRHLLRQKIMGRCTNDFLWATAVVQDVLASVDSSTQKQVLQLVEELPPTHEEMYQRLLFTRLERGNTLRLLQMVSAARQALTADQFRHALAFTQEFKYESITEWEESKEGIEQGEKFENYLRRESRGLIEVHHGPELDETGNSVRFIHGSISTFLRNRRSVVPDETSPWNQRCHLVLLEISLRALDLPGNESVAFVQYACENWIYHVRDCGDLLPEIKELPSFLNDCSKRKAKRIIGRQILALKQTNARQSHLLEAKESLLVLLATLGGNNLLQRHLTECEPCQQDCSTYSTRYRKALQNTIIGRFTDITMWLLEKHVDWDVDINALYEDKTLLYKACYFGQKEVVAFLLTQNADPLKRSLIGYEYPLHAAIELGQVDIVRLLLQHPSASAQFRLPRAAQKSKGYTPLHTAIKSRQPVRQKLAVLRLLLKNAPKGAGTLGLEDEAGVSVLALARQVGKDGGPGAEEIEDEIEDFDEDERNRGRRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFE7</accession>
    <name>A0A9W8YFE7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFE7"/>
    <dbReference type="OrthoDB" id="10579802at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4376010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="149" mass="17098" checksum="E9458730F6DD41D2" modified="2023-11-08" version="1">MTSSSTIKRLSNENSSIEAKEDDTIYMVDNELIARFFDFDRPLSKSAYIRTGKLLQRQQELPNPPSIALRRQIREDGKTFARHNPPKIFTHPAWVEFLKPAPIVVKKEHATARVVTDAMLQKIIADLNEIYRDVDDIIAEKKARKALPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG44</accession>
    <name>A0A9W8YG44_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001711</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG44"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF503">
        <property type="entry name" value="MITOCHONDRIAL POTASSIUM CHANNEL ATP-BINDING SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="2">
        <location>
            <begin position="38"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4376427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="284" mass="30265" checksum="B030763A3079B4EA" modified="2023-11-08" version="1">MDSGSISGLSGVILGTIFSITTSVLVLPALLFSAQAAGQLFSLAPEVTRAKAAAQSVFSLHDEKGTIITATTDLYARPAATEDALLPGASPSYGTFSLKGDLEFRRVSLYYERRPDIAALNNVSKPDLFPGSVKFNIGLGTRPGATISDEDIMQVAKKCEMHDFIMSLPEGYNTEVGAHGSKLSGGQRQRLAIARALIRDPEILLLDEATSQLDASTENAIRKAIAAASKGRTTIMIAHRLASVQHADRIFVFDDGRVVAQGKHDELVAMGGIYTNMVAAQELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGQ3</accession>
    <name>A0A9W8YGQ3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cell cycle RNA binding protein whi3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">WHI3</name>
        <name evidence="6" type="ORF">N0V83_001192</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGQ3"/>
    <dbReference type="OrthoDB" id="431169at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12245">
        <property type="entry name" value="RRM_scw1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000089">
        <property type="entry name" value="RNA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10501">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A/U2 SMALL NUCLEAR RIBONUCLEOPROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="385"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="259"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="311"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="283"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="329"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4375914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="573" mass="60210" checksum="88A3A707F20B7642" modified="2023-11-08" version="1">MAGGPSPSSTSPSNLSNSPFLHGSLSSSTSSSPPKPFSSQNTMTADVFSASHAYGMPNAFGERRTMEKNGLSLLSFSDAETVPEQKGRHTIFLRKLPSNSDRDAVRNILLFAKDLVDCELVSQPRLSEDKGFASAVAHFQTFEGAKEARDLLNGKRNATNDATLVVELVQDGLPGAIGSRRNTVDSAPVRQGSIATGIAATNGRQSSRYNGTFQNMEKMSPPNGTPGLGNGEFPANNLFSPTSPVNTTFASRNLGKSVINDEADDDDGLLNETIGYGSGGPPVQSSIQRRATNPNPSIPVSRFASLSLNTNGVNGMSSPPLPNYASPRPGSNMQSPGTSLSAMSPHTIPSALSNGQNTGFQQYSQHFARNTYPPVNPADQNPPCNTLYVGNLPMDTSEDELKAVFSKQRGYKRLCFRTKQNGPMCFVEFEDTSFATKALSELYGYVLHNSVKGGIRLSFSKNPLGVRSGQGSNMGPPSTITPTTPLSGFGNNVAAPPGFSTATGPPPGLSAPPGLSTPVHNNSNSGFGMYSNGGFGMGPNDMASPMRQPLATSVSGNGFSAMGGGYSAYMMGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH17</accession>
    <name>A0A9W8YH17_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AA9 family lytic polysaccharide monooxygenase</fullName>
            <ecNumber evidence="5">1.14.99.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Endo-beta-1,4-glucanase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Glycosyl hydrolase 61 family protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_002206</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Lytic polysaccharide monooxygenase (LMPO) that depolymerizes crystalline and amorphous polysaccharides via the oxidation of scissile alpha- or beta-(1-4)-glycosidic bonds, yielding C1 and/or C4 oxidation products. Catalysis by LPMOs requires the reduction of the active-site copper from Cu(II) to Cu(I) by a reducing agent and H(2)O(2) or O(2) as a cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;4)-beta-D-glucosyl]n+m + reduced acceptor + O2 = 4-dehydro-beta-D-glucosyl-[(1-&gt;4)-beta-D-glucosyl]n-1 + [(1-&gt;4)-beta-D-glucosyl]m + acceptor + H2O.</text>
            <dbReference type="EC" id="1.14.99.56"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 5">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="5">Has a modular structure: an endo-beta-1,4-glucanase catalytic module at the N-terminus, a linker rich in serines and threonines, and a C-terminal carbohydrate-binding module (CBM).</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.56" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH17"/>
    <dbReference type="OrthoDB" id="6038816at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030248">
        <property type="term" value="F:cellulose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21175">
        <property type="entry name" value="LPMO_AA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049892">
        <property type="entry name" value="AA9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005103">
        <property type="entry name" value="AA9_LPMO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33353:SF11">
        <property type="entry name" value="GLYCOSYLHYDROLASE FAMILY 61-7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33353">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12560)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03443">
        <property type="entry name" value="AA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040930578" description="AA9 family lytic polysaccharide monooxygenase" evidence="6">
        <location>
            <begin position="21"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Auxiliary Activity family 9 catalytic" evidence="7">
        <location>
            <begin position="21"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU368122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4375122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="222" mass="23234" checksum="A2A8D65DEF95AE05" modified="2023-11-08" version="1">MKCVAVATVAFAALAEVAQAHYMFQYLTANGAKGSQYQNIRPVPNNSPVTSLSSNDLRCNVNGATGGSTTTVSVAAGSTVSFTADQAVYHQGPVSFYMSKAGTASTYDGSGDWFKIKEIGPTFTGGQAKWDLEATYSVTVPSQIPAGDYLLRIEQLGIHNPGSPPQFYISCAQVKVTGGGSGSPSPVTKIPGHVKATDPGYTANIYNNFNSYIVPGPKVATY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHB8</accession>
    <name>A0A9W8YHB8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002358</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHB8"/>
    <dbReference type="OrthoDB" id="3438781at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040799079" evidence="1">
        <location>
            <begin position="17"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="277" mass="28174" checksum="54065E428534CF40" modified="2023-11-08" version="1">MSLKFLSLALVAIASASPFESLSKASADEPCEPCQPSGATGTTPPAIGTDLSSLYLDVLASVKDIHFKKRSVESRAEGFCCRQSLNCVNVQSLNIPMCYDKFTTNFAFPDGSYGSLTTGDYSSGSGSANLLTGNYTNGGQSGNIYASDAQAKPNTATLSIPPQYTGTGVGSAVPASQLGSVIVYTTTIPGTTFTAPTTVAETVRVATVSGQTVSETVSATTITQATTIAPQTNVVTKTQDAAATSSSTGAAGQVAVDSTSSLGMSVLGALLYALYAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YI14</accession>
    <name>A0A9W8YI14_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000728</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YI14"/>
    <dbReference type="OrthoDB" id="294702at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45763:SF46">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45763">
        <property type="entry name" value="HYDROLASE, ALPHA/BETA FOLD FAMILY PROTEIN, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="31"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="313" mass="34504" checksum="834F20F41CEBC1FB" modified="2023-11-08" version="1">MSTTFENESITLEDGRTLSYAVYGSPIPVTTIIYLHGFPSSRFEGRLWHPACAKHNVRLIAPDRPGSGRATFQPNRRILDWPSDVLALTAHLKIDQFYILGVSGGSPYTFACLKKIGKDRLLGATVASGLYPIKFGLAGMMIATRILLWVAPWATGLTTTLFDTMIGKAARSKDPKVLEDLIAKDIASRPPADRKALKDPAYWDTFVAMARESFSQGSEGASWEARLLGGDWGFELEQLKVGENGVPLTLWHGTADVNCPVHMAEKADEIMPGSVLHLKEEEAHVGYIFRDVEEILCDLLGLKESEEYLHDGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIN5</accession>
    <name>A0A9W8YIN5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIN5"/>
    <dbReference type="OrthoDB" id="3793790at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="447"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="458"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4378056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="468" mass="51932" checksum="EA903A9A201EF6CD" modified="2023-11-08" version="1">MSLFKSESRKTAIIEDAVGLEQSLIQQAQSQKKGKRQSKQKTTGGFRFSAPQDAGISKQTPAPKAKATPTTAPGKTTAQSKQKTTGGLRFAAPQDAGISKPTPAPKAKAKAKATPTTAPEKTTAKRKLDSASIPTMPPQTTSKMRKLNPIDAPKPTKYSANQASIIPKEQVQDRRVQSQAPQPEIAHHTEAKATPTMAPGKATAKRKLDSVSGPTMPPQTTSKMRKLNPIDSQKPSKYSTNQNPSMMKEQIQDRRIQPQVPASEVAHDTKSGQGGTSQGDEHRSANHHTQTVPKATSPQHPQFEHPKLRYLSKKQSETRALNAKSNLKISQTVERVTDTPTSHKGYEGGRPGHTSAPYLKNGRHGNGGDFDDDPARETGEGHQYEPEDMKRYEGFLQLRHRFYRMHRTYPNLHERERVDPRFKAAWDTEQMWYNRFTEKYPGARVDQWPCGCQKLSEGDESESESEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJI6</accession>
    <name>A0A9W8YJI6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">1-alkyl-2-acetylglycerophosphocholine esterase</fullName>
            <ecNumber evidence="1">3.1.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_000498</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.47" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJI6"/>
    <dbReference type="OrthoDB" id="2363873at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003847">
        <property type="term" value="F:1-alkyl-2-acetylglycerophosphocholine esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10272:SF14">
        <property type="entry name" value="PAF ACETYLHYDROLASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10272">
        <property type="entry name" value="PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03403">
        <property type="entry name" value="PAF-AH_p_II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040786686" description="1-alkyl-2-acetylglycerophosphocholine esterase" evidence="5">
        <location>
            <begin position="23"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4377670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="380" mass="41276" checksum="4349C6499C8ADF40" modified="2023-11-08" version="1">MAPFTTLWIALQVLTLTSTVSTTQLPVVGPGHFEVTFTEFSLTDTNRLDPFAKDGRPRSIMASAYSPSPACQNKRQAPYMPPATAAFQDDKFAVYGLPNGTLKSLTMGTCDKVTKSNSCSPKGFPLVFFSGALGTSRLLYNSMCQSIAAGGYIVVSLDHPYDADIVEFPDGTIITAANISSDADIELAVATRADDIAFVYRQLGNTSLTDKFFPGYPHRNRLYNTAIVGHSLGGAAAAAAMLQIPSLRGGVNLDGTMFGPVVTKGFDRPFLLLGHENKTQNTDPSWKATWPKLTRWKKEFEVKGAAHYSFSDLPLITSALGLQEHLPAEVEQVLGRLEGHRMMDITVTYVTAFLDFLLKSGSEKKLQQASRDFAEVVQVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJY0</accession>
    <name>A0A9W8YJY0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SCH9_1</name>
        <name evidence="15" type="ORF">N0V83_000470</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJY0"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11651">
        <property type="entry name" value="YPK1_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000116">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="167"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="362"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="624"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="220"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="709"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="66"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="262"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="717"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="729"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4377642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="758" mass="84631" checksum="6704066B6952339A" modified="2023-11-08" version="1">MPSSTTLLMPHRTSNNDEETPDQIGSTSSTPPGAMTPRPDPTDKRLPGILHSYFGQVRDSLMPRRSSSAANSSPAAHNFSAQSRPETAPSETQDKEKATEPRPTMLPSPSPSASSRTPSTSQLTGETEKLTEGDFAPPQFQATPPQTPRTRSHESKPLPSNLSRTSNASEKSKESKASPVVGAPKGKLTVTISEGRGLRPSVDPYVVCQFQWAEYISDGPRHDEAKKGRHAPMQMRRTESEMGRPMAIPMKSRQSSNSGHSSDPRENGGLEEVTDPKWEHEAIFDVIGDHAEIDVSVYDRSNGEAFLGHVRFCPNLVEYNEPYDGWFPLEPRDGDGEQVTGEIHLKLNFHKVEKKHYGPEDFEILKLIGKGTFGQVFQVRKRDTRRIYAMKVLSKKVIVQKKEVAHTLGERNILVRTAMADSAFIVGLKFSFQTPSDLYLVTDYMSGGELFWHLQREGRFQEARAKFYIAELILALQHLHEHNIVYRDLKPENILLDANGHIALCDFGLSKANLTENATTNTFCGTTEYLAPEVLLDEHGYTKMVDFWSLGVLVFEMCCGWSPFYAEDTQQMYKNIAFGKVRFPRDALSTEGRNFVKGLLNRNPKHRLGATRDAEELKAHPFFADVDWEALAKKNVVPPFKPKLKGELDVSNFDPEFTNALNGNGSLNARAAALASGVNPASTPLSPTMQANFAGFTFTDQSTMDQQFAHRQRDEMERMDEDEKDDVDWDKPAGRGDRMSGVIPSNDHDIFNHGNFDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CG91</accession>
    <name>A0A9W9CG91_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoserine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">O-phosphoserine phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SER2</name>
        <name evidence="12" type="ORF">N0V83_010799</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily. SerB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.3" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CG91"/>
    <dbReference type="OrthoDB" id="27226at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036424">
        <property type="term" value="F:L-phosphoserine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07500">
        <property type="entry name" value="HAD_PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000143">
        <property type="entry name" value="Phosphoserine phosphatase serb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050582">
        <property type="entry name" value="HAD-like_SerB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004469">
        <property type="entry name" value="PSP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00338">
        <property type="entry name" value="serB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344:SF2">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01137">
        <property type="entry name" value="C1.6.1:_Phosphoserine_Phosphat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01136">
        <property type="entry name" value="C1.6:_Phosphoserine_Phosphatas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00029">
        <property type="entry name" value="phosphoserine_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604469-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4361858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="388" mass="42420" checksum="313614427129E871" modified="2023-11-08" version="1">MMPAKPPPLDVAPSHIVATLFYKSDTRSFFPHPDLSPHSPSDAIPSASLEALKDGVIPTEKLEDYPLEPPTPGPNALDNLYGSYISQSCLTHFFVTLTNIIPGLEQGAIASKRLETSKFCRVVEATFPLPSASPIALETLRRHEAVSRYEREWNVECVFQADSIYRRYKRLAVFDMDSTLIQQEVIDEIASHIGVEKEVSAITAAAMNGELDFEASLRARCKLLKGVPSSVFETLKPRITLNQGVQELIMALKRLGFKTAVLSGGFTPLTGWMGAQLGLDYAFANHLVVSDDGATLTGELTGEIVHAQKKRQHVLDIAEKEGILLDQVICIGDGANDLPMMGVAGLGVAFHAKPKVQMQAPARLNSKSMLDVLYLLGISNEEQQVLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH72</accession>
    <name>A0A9W9CH72_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH72"/>
    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="51878" checksum="F714D1FB88B24040" modified="2023-11-08" version="1">MLSPNDIAPKSKAASIWSFDSGYGSNTFEDDEAFQVNTWFTNARARRMKNSADLLYSPSDDEGHYESGFSSIGSTPMCNNGLPFGYSTQCDRRCSDTSNAGANISDQTAATIARRGKKKNYGQMNTSTPIDNRASSIPPTPVTASTVNEEKEQKTWQCTFCRQHIAPKSWRRHEETQHRPKRKWTCLLTGPRLTVPSRSNTSTLCAFCKAKDPSEEHFLRSHRIAECMKKSEDERTFLRPDHLRQHVKNFHKATLWDIVRDSWRRDGPGKDEAENWTCGFCNQELKTWDIRETHIAGHFKNGLTMADWGEYINPMPTVETTKRRRSSSEEFSSMFTKLARTFTGRSSHQLEQPSPSTYQFANAWQPLPTATRPMLPNTPLLPDMVFDSFMAEVSGNCFDTANPTTAGLEQVDPNPNNRYDSVFPDDSTMELDFDMLADAFLNGSTMEFQGPWDQRQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHE3</accession>
    <name>A0A9W9CHE3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxypeptidase</fullName>
            <ecNumber evidence="6">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_010359</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHE3"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF453">
        <property type="entry name" value="S1, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4362266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="344" mass="39011" checksum="66748D545713AEB7" modified="2023-11-08" version="1">MIGLFQENGPCEFELGSHNTMPINNTFSFNNNVNMLYIDQPLEVGFSTGNGSVNSTDSAAPYVWNLLQAFYASFPDYQSRDFGIFTESYGGHYGAEFAQFIQHQNNMKVGEHIDLIALGINNGWHDSMIQEPAYVDYAYNNSYKPFISEAQHTRYHQYFEDYCLPDLLLCNQTGSDFDCTTADRACNAVVENPLYNYIDFNLYDIRKPTNDSEPPENYLKYLQDPDVQRAIGAKTQFVECSDEISRRFISTGDNSRSYLDELSEVVSSGITTLIWAGDADYLCNWYGGHDVANAVAYPGQSAFSTKSLEPYKVDGKEKGSFKTEGNLSFLRVYEAGHEVMYYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHG2</accession>
    <name>A0A9W9CHG2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Arabinan endo-1,5-alpha-L-arabinosidase</fullName>
            <ecNumber evidence="4 7">3.2.1.99</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_010552</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Endohydrolysis of (1-&gt;5)-alpha-arabinofuranosidic linkages in (1-&gt;5)-arabinans.</text>
            <dbReference type="EC" id="3.2.1.99"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Glycan metabolism; L-arabinan degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the glycosyl hydrolase 43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.99" evidence="4 7"/>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHG2"/>
    <dbReference type="OrthoDB" id="195678at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046558">
        <property type="term" value="F:arabinan endo-1,5-alpha-L-arabinosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18831">
        <property type="entry name" value="GH43_AnAbnA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050727">
        <property type="entry name" value="GH43_arabinanases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006710">
        <property type="entry name" value="Glyco_hydro_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016840">
        <property type="entry name" value="Glyco_hydro_43_endo_a_Ara-ase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43301">
        <property type="entry name" value="ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43301:SF3">
        <property type="entry name" value="ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04616">
        <property type="entry name" value="Glyco_hydro_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026534">
        <property type="entry name" value="Endo_alpha-L-arabinosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040755397" description="Arabinan endo-1,5-alpha-L-arabinosidase" evidence="10">
        <location>
            <begin position="20"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity, responsible for pKa modulation of the active site Glu and correct orientation of both the proton donor and substrate" evidence="9">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR026534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR606710-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR606710-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4362459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="345" mass="37497" checksum="0373147984D6F325" modified="2023-11-08" version="1">MWDLRKTAIVLGLLSVSKALTFRQTSSYPPPEPCTGNCTFIHDPSVVRRSDGTWLRFSTVGNIAVATAPALTGPWTYRGALLPQGSTIKLRDDQELWAPDVFFSNGQFYAYYSVSKSGFQGSEVGVATSVTGDVGTWTDHGSVGVPKSTDYNIIDPNFFRECDTCDNVWTFGSAWQGVYQTSLSSDFLKWSGEAPHQVAYNSSHPAHPQDYPSITEGGFLFWWEVSGTKYYYLFFSSGACCNAANDLEAPGDEYKIMVCRATSPTGPFVDANGKNCASGNGGTLVLGSHGNVYAPGGQGVIVDPAVNRVALYFHYANPSIGYAFNQFLFGFNYLDFSSGWPVVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHP2</accession>
    <name>A0A9W9CHP2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Amidohydrolase 3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_009582</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHP2"/>
    <dbReference type="OrthoDB" id="10258955at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008448">
        <property type="term" value="F:N-acetylglucosamine-6-phosphate deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006046">
        <property type="term" value="P:N-acetylglucosamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01309">
        <property type="entry name" value="Met_dep_hydrolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-PHOSPHATE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113:SF14">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-PHOSPHATE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Amidohydrolase 3" evidence="4">
        <location>
            <begin position="460"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4364127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="966" mass="104984" checksum="2B8AAFEB9EC29767" modified="2023-11-08" version="1">MEKHALEGLPTYTDATTSSRPRPWHNRRKRQRTFRVLALSCFVYLAYYTFTIASNTAKPINTLSLERLQEDYATCSKLRSKPAEAYETRETNKRWINTTKPLLIRNATIWTGEPTAGTSDEDARAGSGWSWTSSDVFVDKGLIFKIASSIDVKHLPHNTDIFDANGRQLTAGIVDMHSHAGLGSVGNLNDDTNEMSSDVTPYVRSLDGIDPLQPEIEFIKSGGVTTSLFLPGSGNNIGGEAFVLKFAVGTKNGRAEISQQDMLADPDRNWRYMKMACGENPKRIYGKAGERGPYSRLGEAWQFRHALEQAAKYVREQDEWCAAADTFGVEKMTTHLKQELEWESLGAVLRGQVRINTHCYTIPDLEAFVRHTNEFKFRVYAFHHAHQTYLVPEILERAWGGTPAAALFADNMYYKVEAYTASERAGAILYAHNITPTYVSDNPVLNSQHVVVEAAKAYRNGLPYHAALAGVTSAPAELLGLGQRIGKVKPGFDADLVVWDSDPLSVGATPVQVWIDGAKQFEHPVELKKPILPPVAPNVELAKELKRVDIAGDLIFTGISHVHHHAFLDAEKHDGDVIAITSNGELICVGRCESELAVLSSTGTPPKTIKLKNGYLTAPLTAFGTALGLQEIDAESDTHDGPPPSDGLTFALDAISFGGKQLAAAYAHGVTKAISAPILDGIDSRGISVGFHTGAKTRLEKGAIWNPEAAVHYPLTLNAKNDKTPSITSALGDLTFKVLDAIDELQKNSTSTDLPSKERLQESAYLRRVVQGDIPLVLSAHSADTIAAIIEWKKYIESTITTSESTTISPSKAPPLRVIIIGGAEAHLVARDLASAHIPLILAPLLPHAQTWDQRRGLTGPPLTNVSTNILLDAGILLGISVEETWESRDLGLLAGVAFANGEGRLSEGEALGLVSGNFEKMLGVGVEEGMGRRGRASGLFGRGVRLRLRGLLGPLGARGRTSVWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIA7</accession>
    <name>A0A9W9CIA7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_009430</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIA7"/>
    <dbReference type="OrthoDB" id="2148442at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003939">
        <property type="term" value="F:L-iditol 2-dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006062">
        <property type="term" value="P:sorbitol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05285">
        <property type="entry name" value="sorbitol_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000068">
        <property type="entry name" value="Sorbitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045306">
        <property type="entry name" value="SDH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161:SF4">
        <property type="entry name" value="D-XYLULOSE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="10">
        <location>
            <begin position="58"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="9">
        <location>
            <begin position="209"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4363976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="394" mass="42049" checksum="43AB05B0BC2902E1" modified="2023-11-08" version="1">MSPAIVLDPPASNTYVTKTLSQPATGTTIKTPLPNPSLQVTADHKLKQEEAPVYAPAKGEVLLHIKATGVCGSDIHFWKTGRIGSLVVEGDCILGHEAAGVVLQCGEGVTSLKPGDRVAVEPGVPCGDCFLCMDGRYNLCEDVQFAGVYPYHGTIQRYKCHPAKWCHKLPSNVSFAEGALLEPLSVVMHGIKSAGLSLGRGVVICGAGPIGLIALAAARASGAHPLVITDLEPKRLAFAKHFVPSASTYQVDRKLDAQGNAQKIRELFDFANMGEYGAPETILECTGVESSVVTAAYTARRGGTVMVIGVGREIMNNLPFMHLSLAEIDLKFINRYRDTWPTGLQCLAGGILDLKPLVSHTYPLEKALDALHTCGDLSNGSIKVQIIDEQENVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CII1</accession>
    <name>A0A9W9CII1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CII1"/>
    <dbReference type="OrthoDB" id="3755745at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="218" mass="24082" checksum="419570B785BF91FF" modified="2023-11-08" version="1">MIEQDSFSLARESSHHPASTFTEHGDITRQNHSQKSSIESNAALRDLIKPWGALPFTPQSCRTVSFSRWTRFTASSTTSTPTSELKASTLCKASGLAPTYDPPTSEWVYLGADQPVNVVEPRSVLRQFEHRRQRSSASWATVSPASDGGEQSKPIQFRKQEVKPVNISSQLSNSFRTSPGACKQPSTMKMMLQRLGLENATCEELPSPFESDSEGEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIJ0</accession>
    <name>A0A9W9CIJ0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008973</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIJ0"/>
    <dbReference type="OrthoDB" id="5689at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050832">
        <property type="entry name" value="Bact_Acetyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43877:SF2">
        <property type="entry name" value="AMINOALKYLPHOSPHONATE N-ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43877">
        <property type="entry name" value="AMINOALKYLPHOSPHONATE N-ACETYLTRANSFERASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="7"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="179" mass="20420" checksum="4BFC3911208AA3A2" modified="2023-11-08" version="1">MTTLSTTVFRLATEQDAIPLERLINTAFRNDTTTQVYLSTDHERIGVTSVESIKARIDQPDFTVYVATNLAGELLAHGSVRKLDESIAWFGMLAVDVIFQHRGLGSQMLAYAEDFARRELGCTRMELNAVNTRVDLIAWYQRKGYREIGVTTPFPYEFHGDWNGVLRDDLRFIHFGKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIL1</accession>
    <name>A0A9W9CIL1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_009570</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIL1"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000090">
        <property type="entry name" value="MFS sugar transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF80">
        <property type="entry name" value="SUGAR TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12170)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="363"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="398"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="16"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4364115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="497" mass="54378" checksum="3736FA78376DC411" modified="2023-11-08" version="1">MVSSIKPRVNAYTWGCGLFAAVGSLLYGIDSGIVSTTIAQHGFTDYFAPFTPSLKGALVSTFGAGSVFGVFFAGWSADFLGRKKTIMIGAIIALIAGIIQAASVHIGMLIAGRIIGGFAVGIMNMTIPIYNSEIAPPHKRGMISGLHAQFVGIGFATANWVGFGCSYATGQFQWRFPLAFQCLPAIIVLIGIFWLPYSPRWLLEQDRDEEAYAVIKRLHGNTGDDSFFRAEFTQMRDQLRFEKSVQKAGWKDLLNTPSNRKRVLLAVLVQAFTQLSGINVINYYQTDLYKGLGQTGHMVTLLAGVYGLVGPIANVICLRYVDTWGRRSTLWITGLIMTLDMVLVMGLSGAYGSSSNTVAKGFTIAFIFCFSIIYSLGYNSIHYIYVPEIMTMAIRAKGSAVSIICNVLINIIFNQVSPIAFADVGYKYYSLFICTNLVGAITVFFIFPETKGKTLEEIAHIFGDEVIVADLATVKEKVGHEEDGNEVELVEDKNKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIW0</accession>
    <name>A0A9W9CIW0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIW0"/>
    <dbReference type="OrthoDB" id="3784677at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="202" mass="21378" checksum="9553332E237EEA5B" modified="2023-11-08" version="1">MPPRTPKKDTSTSTANANKTSPNNNNTNTTNTELFDLGRDRFDHDTALPVDRDYALNTARPVVVRSGRAPKGLENKVYEWSTFQFTTKERDEREELKGDLHEGQLHRQNRAAREGGDGGGNGMGMGGMMGGGGDGRRGGGVEGGMGRGGRGGGRGGRGGGEGGGGRVDSLRAGMKPEIDAPPMAKGFAEELDKSMFWDGPIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJL3</accession>
    <name>A0A9W9CJL3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uracil DNA N-glycosylase Thp1</fullName>
            <ecNumber evidence="6">3.2.2.29</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">thp1</name>
        <name evidence="6" type="ORF">N0V83_007813</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.29" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJL3"/>
    <dbReference type="OrthoDB" id="565731at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141016">
        <property type="term" value="F:G/T mismatch-specific thymine-DNA glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10028">
        <property type="entry name" value="UDG-F2_TDG_MUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.470.10:FF:000010">
        <property type="entry name" value="G/U mismatch-specific DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015637">
        <property type="entry name" value="MUG/TDG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12159">
        <property type="entry name" value="G/T AND G/U MISMATCH-SPECIFIC DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12159:SF9">
        <property type="entry name" value="G_T MISMATCH-SPECIFIC THYMINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="5">
        <location>
            <begin position="115"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="55"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4366178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="326" mass="36450" checksum="231E89BB96AAFC3D" modified="2023-11-08" version="1">MPRTRSTKSEVAGQPSHASAHEVHLSDSAETPTASLPMSFKGQLAKYQFTEIDTVSRNGTSDVESPRATKRTRTTVKIATDRKSTESRPKKKRKPSKFAEPSKYAHLSPLVDILEPNLICVFVGTNPGVQTAIAGHAYAHPSNHFWKLLHSSGLTDRRLKPEEDRSLPALYCMGNTNIVDRPSKDAAELSKEETAAGTANLDAKFLKYKPEAVCIVGKGIWEAIWRYRYNKNMAKAEFKYGWQDEKHNMGKSKQGEQGMDEDGNIWKGSRVFVTTSTSGLAASLKPAEKEAIWKPFGEWVQKRRAERGFVPRPIEAEDATQEWEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJL4</accession>
    <name>A0A9W9CJL4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008478</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJL4"/>
    <dbReference type="OrthoDB" id="3262926at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4365856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="175" mass="19787" checksum="8B3944FCD04BDF71" modified="2023-11-08" version="1">MDATWEEVERMATAASADDAQLASQYPTPETVERWTHLFGYSYMEAVKLISDQRGDVTRERITDDHWALIKDEKEALGYDREAYEHSLQLSQVFKGQSASIPAGGGSDDDDMMFLFRLGGLLKTPEKVKEVAGLEELPVVREGTNEMGVVKFCVVDKDTQKKLEEWLTQHSVLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJR6</accession>
    <name>A0A9W9CJR6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_008524</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJR6"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000180">
        <property type="entry name" value="MFS monosaccharide transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF61">
        <property type="entry name" value="HIGH AFFINITY GLUCOSE TRANSPORTER RGT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="325"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="349"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="416"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="461"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="488"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="29"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4365902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="570" mass="61678" checksum="C5047F5538D465BA" modified="2023-11-08" version="1">MPGGGVVPVTGTADVSRVEAPVTVRAYLIVAFAAFGGIFFGYDTGWMGGVLNMDYFIKQYTGAEYPDVKFPGLSSSDALVTDYRKTTFSVTSSQQSLVTSILSAGTFFGAIVAGDLADFIGRRFTIILGCLIFTIGGILEVASTGLGVMVAGRLIAGFGVGFISAIVILYMSEIAPKKVRGAVVAGYQFCITIGILLANCVVYATQNRRDTGSYRIPIAVQFLWAIILATGLALLPESPRYWVKKGKLDKAAASLGRVRGQPLDSEYIQDELAEIIANHEYEMSVVPQTSYLGSWMACFEGKISSPSSNVRRTTLGICMQMMQQLTGINFIFYFGPVFFQQLGSISNPFLISLVTTLVNVLSTPASFFLVEKVGRRPILIFGAAGMVIMQFIVGAIGCTAGKNSPDHPADPAATRAMIAFICLNISVFAMTWGPAAWIVIGEIFPLTIRSRGVGLSTSSNWFWNCIIGVITPYLVADRADSAKLGSNVFFMWGGLCCISFLFAYFFVPETKGLTLEQVDKMLEESTPRTSRKWKPHSTFAAEMHLGEKNIEIPTGHHHENVGAKNEVSTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJV8</accession>
    <name>A0A9W9CJV8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ZZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_007742</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJV8"/>
    <dbReference type="OrthoDB" id="661148at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="5">
        <location>
            <begin position="355"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4366107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="527" mass="57756" checksum="599867DA3C94401F" modified="2023-11-08" version="1">MPPAPPTTTSEQQLREDEMLAHKIQQMEVEELRRRSSSVVSQHPRLGSMAPPSPQAPSSLLHQPSSQSLRPHSESVSSTVAPYNTETILGSPPRQASPSLLPEVVAGPSANPYFPQPISENLPIPVLQDQITPHLPSAPLDPASLSAYLEQHRQPPYPPQWVPPPVFASFYAYQGANIAPGSSWLDTPDSVVWRAVRPQQHARNPAPASYTFRFKTSSGTLRSPKHSWAMTCPGEQADLLKKVSKSTRPMWSYDLKLDSRTGLRKSEVLSHGREKAILTTYVHALNYDSLRFIGPDNRAYMWVSSSKLSSLNGSRYDTVRHALFVATGNVPDPLYGQIVADHTFWDGYIDVNEVHVGIKCKGCDMNPIRGLRWKCRTCPHHDVCDACRALALREQFGALIQPTCDFSLVNLPDESLHIRSPDVNPALVVATLQIFKDWEKHTLREERKKNVTGFMASEEAARKCDLGVMSYWKAGDLDKKGAEGERFGTMIKARGIMQTVGETAGALGSVADAGVAVGGDGGGGSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJZ9</accession>
    <name>A0A9W9CJZ9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protein-lysine N-methyltransferase rrg1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rrg1</name>
        <name evidence="1" type="ORF">N0V83_008041</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJZ9"/>
    <dbReference type="OrthoDB" id="433955at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF156">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE EFM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4366405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="343" mass="37470" checksum="40B1E754B7BB093E" modified="2023-11-08" version="1">MSLIDVLDLPQLYTRPSAEALLNTLALLRSAPPSWDRDNIDGGGEQTRAQVSPEGVTRYLTSIVSSSLKWIEDEEAKEEIWNQASSRLSERSGRSAMGAMSRKFRIPSSSGSFELSIHEPALTGDDLGLKTWAASYLLAKRLHTLNLIPANTNEKPQVLELGSGTGLVGLAMAALGADVLLTDLPSICSNLAHNAQVNSEIVNRNGGSTRTAILDWTDPTSCKLCSDAAVEDEALASKFPLILAADSLYSPQHPQMLVDTIDVWLSADINAKVIVEFPYRDAYLPEVQDFRQRMSGIGLQIIEEGEEKGYDDWGESGANIDQDESALVTCWWSCWGRQMSHTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK50</accession>
    <name>A0A9W9CK50_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial</fullName>
            <ecNumber evidence="6">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="6">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LSC1</name>
        <name evidence="10" type="ORF">N0V83_007357</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer of an alpha and a beta subunit. Different beta subunits determine nucleotide specificity. Together with the ATP-specific beta subunit SUCLA2, forms an ADP-forming succinyl-CoA synthetase (A-SCS). Together with the GTP-specific beta subunit SUCLG2 forms a GDP-forming succinyl-CoA synthetase (G-SCS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 8">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK50"/>
    <dbReference type="OrthoDB" id="1664372at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009361">
        <property type="term" value="C:succinate-CoA ligase complex (ADP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004776">
        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000005">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004230">
        <property type="entry name" value="PRK05678.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117:SF2">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP_GDP-FORMING] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117">
        <property type="entry name" value="SUCCINYL-COA LIGASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01798">
        <property type="entry name" value="SCOASYNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA-binding" evidence="9">
        <location>
            <begin position="35"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="6 7">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="48"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="127"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4366831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="328" mass="34554" checksum="E67ECF7230640726" modified="2023-11-08" version="1">MQAIRQPLRSALSKTATRQYATNSSQYAETIKNLRINGDTKVLFQGFTGKQGTFHAQQAIEYGTKVVGGTNPKKAGTEHLGLPVFKNVAEAIKETQASATAIFVPPPVAAASIEEAIAAEVPLIVTITEGIPQHDMVRITDMLKSQNKSRMVGPNCPGIIAPGQCKIGIMPGFIHKRGRVGIVSRSGTLTYEAVNQTTQAGLGQSLVVGIGGDPFSGTNFIDCLKVFLKDEETDGIIMIGEIGGSAEEDAADFLKEYNTANKPVVSFIAGISAPPGRRMGHAGAIVSGGKGDANSKITALEAAGVTVERSPAKLGSTLYDQFVKRDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK72</accession>
    <name>A0A9W9CK72_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007342</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK72"/>
    <dbReference type="OrthoDB" id="5357734at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021514">
        <property type="entry name" value="DUF3176"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37576">
        <property type="entry name" value="DEFECT AT LOW TEMPERATURE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37576:SF2">
        <property type="entry name" value="DEFECT AT LOW TEMPERATURE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11374">
        <property type="entry name" value="DUF3176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="127"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="560"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="694" mass="76772" checksum="D081303D4049AAC3" modified="2023-11-08" version="1">MANTEHDIRPISEAPTVVNKVIYPDRHSWKDSPKSSKGDLVIDVEQLEEKKEVPRNAEESNDKAHVDENDDGIDWRPGFKQQFPWIGFAGLMTVIIATALAVAILSQSHKKRVKDWPVKRFPTQPNVLLNIANQVQNLGLITLIGQGLAIAWWRKALRGSSLKTLHQNHAYSYSFYAIVTSGRHFNIIALAALMTKFAVIDSTLFQKATKTEITQQVAYANTSVTGWIETNWQPNRGGIPGDEGNIKTVDAAWASVLDAYNGKIANGKVHDLLEGNASFFDCPFRQECSGNIKGLGFAFNCSTSVEDVDYGLQHKSQHGGVSTSYPLWDINFNSSWSNETKPYASILLDMLYVDSHAGTTNGSCPGILTRRSCEIRPAVVQYPLTVMMPSQEELQGKNIVTHVKFFEDNKTWPISSPLADTEQIDMLKVIDYVDLPEHFGQTSTVGALTYVLNNLYSSSANLTYEHDWDIVARGSSAQTTFFADSDTEDFSRCWYALDRAGKDDPAIELLRKINTLSFVAALYLNGAPTTDVTQRAAAGMSSQTILTSVTGIVEEYMTSFAYVGGALAATLTTLLLVLPVYWGFWQLGRKVTLGPLEISHAFGAPVIAPERMKAHHAEFEEVLGEVGHRRVRYGQRVDAPPGHMGIAEPDRVVEPAARKSWRVGDRGPRGCWDWGGDWGLIAAAIGGMRKIRSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK87</accession>
    <name>A0A9W9CK87_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008118</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK87"/>
    <dbReference type="OrthoDB" id="73680at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037370">
        <property type="entry name" value="Pleckstrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12092:SF16">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12092">
        <property type="entry name" value="PLECKSTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="185"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="427"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="442"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="452" mass="48719" checksum="FEFA130E60EFFBBA" modified="2023-11-08" version="1">MAQYYGTSTKGDRSSSAFDFVRPLKPTADPNPTVTKLTYDSHSIDAIANSPNMPGSETSSTGRASPSPAAMTHSRSFFSKKSQPRGLRAISDAITGGEKANVSAGDVTTLSNKDSPMQSPTRTGSSTPSVTEVSNSLDLGKDASKRLTGSSSATKDGARKKSKKHGTSAYLKGRQQITPQEAMANCDYSGWMKKKSSSLMTTWKTRLFVLRGRRLSYYYTENDTEEKGLIDISSHRVLPANNERMTGLHATFTRATSSPSSPQNATIQTAAATDSANGIPGMEDDMSGMFIFKLVPPRAGLQKGVQFTKPIVHYFAVDSLAVGRLWMAALMKATIDRDEALPFTTTYQQKTISLEKARAMRQRPPNLMDDDDDTETGSAENEKKSEYEKESIQESVIHEEDEKGLNIAGLDGAKALLAFADGADETAATAVPTEKENKHDSTATTTATATTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKP1</accession>
    <name>A0A9W9CKP1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ATP-binding cassette, regulator of translational elongation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">GCN20</name>
        <name evidence="7" type="ORF">N0V83_006931</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKP1"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000104">
        <property type="entry name" value="ATP-binding cassette sub-family F member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000882">
        <property type="entry name" value="Translation initiation regulator (Gcn20)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF117">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="200"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="532"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="771"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="839"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="125"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="283"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="863"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="884"/>
            <end position="897"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4367350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1258" mass="137742" checksum="D74CAD9486299BA5" modified="2023-11-08" version="1">MEAEIRTQIPNIDPVLSEYSVGYLTHAANSFSSDSDPNAPSPLEEAAATVSALLLSASGDLSDQNETSIQNLVEKFISRLNAANGTDGERRQMAPSAKKLEQAIHVGSQRNISSTLGLAGGAVDLESANTRKVESRVDRKKLEKAERKLRAKQDRKEFKNVEYEASRLLNIPDEAQSYEEFYMAVNPLQLGDSASKSKDIKIDSFDISISGKRILTDSSLTLAFGRRYGLVGQNGIGKSTLLRALSRREVSIPTHISILHVEQEITGDDTPALQAVLDADVWRKHLLKEQEKITKELAELEAERATMADTSADAARLDTQREGLDQTLSDVHSKLAEMESDKAESRAASILAGLGFSTERQQFPTRTFSGGWRMRLALARALFCEPDLLLLDEPSNMLDVPSITFLSNYLQDYPSTVLVVSHDRAFLNEVATDIIHQHSERLDYYKSANFDSFYATKEERRKTLAREYEKQMGERAHLQAFIDKFRYNAAKSSEAQSRIKKLERMPVLTPPEAEYTVHFKFPEVEKLSPPIIQMSGVTFGYNKDNILLKNVDLDVQLDSRIGIVGPNGAGKTTALKLLIGALQPTTGLISQNPRLRIGFFAQHHVDALDLNDSAVGFMSKKYHGKADEEYRRHLGAFGITGMTGLQKMELLSGGQKSRVAFACLALTNPHILVLDEPSNHLDIEAMDALSEALQKFQGGVLMVSHDVTMLQNVCTSLWVCDNGTIEHFDGTVKDYKRRITAQANEAGVRLLAPLPLRGSSSTLRPIHAATGTLSPTQAPSRDGLSVVTLLPPTPSDSDTQRPPYAATAPTSINAVLTTAGESPYPPPFSSLYFPTEDAVEARNKPCDSSEDDSPPAFAPAPPFTESSSSASAAAAAAAATKAALPRDTKDGSSSKDLDDGEPPPPYSEGSSPLESFTYVMASAGGPASIITQVSQSSAGPPINTLGGSDENITLELRGTRFTLSRDELLTLPEFVLLSLFPNGLLPDGHMNSYHDGDVYPVDVGNPDFPSPSPPPPVGPMAKIMYHLSNPALYLSDNMYPSQYDPNSLQYMLEFFRTVAQTIPASPPSPTAAPEHPTEAVPIEPMHGSARDMLQDRAGIIVLREDLDFYAIPPHRDITQPEMIEVKRAAGEALLRQDGIFSGLRKSEEPGTTEQHLIEMLTAGGFNHDDHWGHRAGEPNKAVICSIALARLRTDIKGNDLANSNAVGMAQKLLLFWRKPARRCWWEGVELDNVRGVEGKLKVWIRRVWTLEMSVIGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKU1</accession>
    <name>A0A9W9CKU1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Chloride channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_007083</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the chloride channel (TC 2.A.49) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKU1"/>
    <dbReference type="OrthoDB" id="44789at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03684">
        <property type="entry name" value="ClC_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1280.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711:SF3">
        <property type="entry name" value="CLC CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7 9" id="KW-0868">Chloride</keyword>
    <keyword evidence="5 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="109"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="365"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="11">
        <location>
            <begin position="563"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="19"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="32"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4367500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="624" mass="67383" checksum="D57870E1A0797093" modified="2023-11-08" version="1">MALAFGVISSSATMLTKSSLPSASSSEQSSSATTKPNGADIPNSSTTPATAPAGKVMYMAAGSGIPEIKTILSGFSIPSFLSFPVLAVKAFGAIFAVSTGMCLGKEGPFVHISTCVGYLVGMLFPKYRDNGRKMRELLSAACSSGLSVAFGAPIGGVLFSYEEISTYFPRKVLWRAFLCSLTAAMVLKELNPTGTGKLVLFETNYGTDYKPVHYLAFLLLGVAGGIFGGLFCKLNFLWSKWFRAFTIIKKNPVLEVFLVVLATALLQFPNPLTREPGDIIIKNLLVDCNDNSTSQHSSWVCRNELSDETSWPYIGYLLYGTLAKLVLTIITFGIKVPSGVIIPALDAGAFFGRLIGQLIPSISPGIFAMVGAAAFLAGVSRMTISLAVIMFELTGQLSYVVPHMLAILVAKWVADAISEEGVYDLAQTVLGHPFLDPDVAIQIVRKRKMCAEVLVPPQRTMQEITVLVPGTNKVAYTLLKEKLAKLKERGLMDAGLVLVQCHGDSSIPILQGYISQSELEFGLTRLVPATIPGQSDFQIRLLGHEVDDAPSPDALEVDLTPFVDRTPLSICAKAPLEYVVEMFSKLGLRYLCVTEEGTGRLVGVIIKKRLVGFLEHLREHNEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL42</accession>
    <name>A0A9W9CL42_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutathione S- transferase, nitrogen catabolite repression regulator</fullName>
            <ecNumber evidence="4">2.5.1.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">URE2_3</name>
        <name evidence="4" type="ORF">N0V83_005840</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL42"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03048">
        <property type="entry name" value="GST_N_Ure2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF23">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-LIKE PROTEIN TPCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="8"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="96"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4368758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="231" mass="26042" checksum="9C6EAB8638DF9681" modified="2023-11-08" version="1">MSSSTSTLKPITIHGKRGPNPPKIAMLCEELGLPYEALDTEHASLKTPQFLALNPNGRMPVIHDPNTGYTIWESGAIILYLMERYDTDKKLSFPQGSEESYLAQQWLFFQVSGQGPYYGQGVWFTRYHAEQLPSAQERYYNEIRRVTGVVEGHLKKQRASGEEGKDEGPWLVGGKFSYADLAWFSWQNLTMELLAAKVDLSEFTETAGWIGRMKARPGFKKVLEESAIKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLJ9</accession>
    <name>A0A9W9CLJ9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">hydroxymethylglutaryl-CoA lyase</fullName>
            <ecNumber evidence="3">4.1.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_005888</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(3S)-3-hydroxy-3-methylglutaryl-CoA = acetoacetate + acetyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:24404"/>
            <dbReference type="ChEBI" id="CHEBI:13705"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="4.1.3.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate metabolism; (S)-3-hydroxy-3-methylglutaryl-CoA degradation; acetoacetate from (S)-3-hydroxy-3-methylglutaryl-CoA: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HMG-CoA lyase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.4" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLJ9"/>
    <dbReference type="OrthoDB" id="1905920at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004419">
        <property type="term" value="F:hydroxymethylglutaryl-CoA lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046951">
        <property type="term" value="P:ketone body biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07938">
        <property type="entry name" value="DRE_TIM_HMGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043594">
        <property type="entry name" value="HMGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42738">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42738:SF7">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="8">
        <location>
            <begin position="122"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="46"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4368806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="280" mass="29507" checksum="1CCB6AE6FFEE6431" modified="2023-11-08" version="1">MANSAEILEHVLNNTPQSPHPITYQWLLPNVRGLDNFLALWQARTQSGQDAYPTPPPSPGTNEGPPLNTSSQDANATPSATSSPPSHELSIFTAATESFTQKNTNCSIAESLTRFEPIMTRGKELNLKVRAYISVALGCPYEGPNVDARKVADLAVSLLEMGADEISVADTTGMGTAPKTAELLKTLNAAGVDKTDLALHFHDTYGQALVNSVVALEHGIRTFDAAVGGLGGCPFSPGATGNVATEDLVHCLHSLGLKTGVDIEMLAEIGEWISGSWGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLV2</accession>
    <name>A0A9W9CLV2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CBM1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_006054</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the CTF8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLV2"/>
    <dbReference type="OrthoDB" id="70316at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030248">
        <property type="term" value="F:cellulose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000254">
        <property type="entry name" value="CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035971">
        <property type="entry name" value="CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018607">
        <property type="entry name" value="Ctf8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28605:SF1">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28605">
        <property type="entry name" value="CTF8, CHROMOSOME TRANSMISSION FIDELITY FACTOR 8 HOMOLOG (S. CEREVISIAE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00734">
        <property type="entry name" value="CBM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09696">
        <property type="entry name" value="Ctf8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00236">
        <property type="entry name" value="fCBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57180">
        <property type="entry name" value="Cellulose-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51164">
        <property type="entry name" value="CBM1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="CBM1" evidence="9">
        <location>
            <begin position="156"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="194"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4368972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="384" mass="41081" checksum="3A79F25B8A451639" modified="2023-11-08" version="1">MPSITLYPPSKSASATSPTENPLPALLHTPSGLALLEIQGTIHFPAATSASPSSTQVGKFVFPLYDASNDPSDTKWMKRVYLYVGKGQRMTGECKKLGKPVGVVRKRERGEMGKDRDHGVIDREDVEMGGVDGQGEQRAIEEELEIVEVVRKDKTTKNPPPTLLGGGASWTGETACNSGAGCVVINDYYSQCQPGAAPADPQPEPSAQPTEVTPVDTPTATGAPAGTGPGKTLQSGYYWIRAVAAPNFHKYIQTNPLYSTGPALLGDYTTAGQFQVVDGQLVQLVSAPGEAVKLLYAVVNETRVINDNSLAVSFSETKNTYGTFAFGGDDLQWSVAGVTRPNKSAWYVCTGQKLYVNLGNYLYQTPSGCADQTVHYYNDKTANN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLZ9</accession>
    <name>A0A9W9CLZ9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005402</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLZ9"/>
    <dbReference type="OrthoDB" id="3886371at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="260"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="413" mass="45854" checksum="D78AF1558E014577" modified="2023-11-08" version="1">MSPYQQIFSTNAILSVWRSLRTTRDHNDLYLTLQYHADDVRGQNGKQYGKGGAVEGAQEVTDGKTVDVGGKSIGFVVPWRSMQAQRRELREKEKELAEALVVLAGEVAFTDLSPTLQSSLTQNPLIQIRHLAAGLSRQMSKIELQMADNAPFANGKNLYTGGEIAQKFLPTIQTLFEQRPVPQRMIFELLMELKDLVYMGMAGCDKEVLEWEIDGAGTGALEELDDAIVRAVTPLSIMEGGQQPQEKEPGHKRLLKRKPSSMSMKSSSQQQQPPSPLLAAPEDLLYALESLETTALALTHLPIPIQDFSAHAIITLRRLAPRQTLADFSQQKKKRAGRCAEYARCMKWAGTSWRQGGGCRRGCKNEDEDPENLPSWHRSSRWFDEKGSGTVMVGGGRAKARDDSVMDMRVLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-10-08" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMH3</accession>
    <name>A0A9W9CMH3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_004753</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2546325at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF9">
        <property type="entry name" value="NACHT DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_2G01760)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="3">
        <location>
            <begin position="330"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4371534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="2098" mass="236980" checksum="D1B39C8F000E7848" modified="2023-11-08" version="1">MSQRVSSAGHLNGLNGNNVLSPPQSPKAAFRSKGRLPSFTSSRAPSGNFSTFKSFNKRSNGASSYKIGFVQESAISLKKRSMAELGAGVYQGISDVSYVKFLEWIRSERLTTLPHKGSRWDKVLIRSLYFAEQLHEFEQAIQSFALDSSSAAAIGYGHAQLLLELSHNNSEALDKAFSVFYKFAMSFASVLQRSELLAATPEIRENLCLLYTDLISLVVNVGIKFYKTVNGMTSGRASLDIFELFGETIDTFRTRQNTVIELIWDSQIENEDFEEEEALDVKVLSRWLAPEDRVLAALNRDHSTFVDNQVEFTCLWFQKHLSKFVQSDHKFLLVTGQPGAGKTTLAGSIVERLQRPVNRRQFDTLFCSLSPDIPTTATSLAVVKTLLFQLLNQRVGNMGMYYALFRAYHQCRTSDDLKSYEEHLWQALADALQHPVEGSNELLIVVDGLDEIAESTSASIQASGAISPAALLEKLVNVTNQGHGVRLITLSSSVKLPSSAKGTQHQISRDDIRDDIHAVALRALVHNHHFHGQRAYDQEQLLDRIIQATNGSFLTAILACEILNAQKSPEALVKTLEGFETTKPSAQDLILKLFSSLNPTNNAKTLLSWVLAAERPLTIDEIHILFTVDVKSGTLSDRGVNVNETLNSLKSIFTLHERIVRFRHPIIHSALHDFASQNKIPIPLKESEMDLLLRVLTYAKHTLKDKGEPTLDVSDPGVMDRIYHQHHFLEYTVRYWVLHLQQSPLAPKTPEDFKPTADLQKAFPDTTIFPILESLCWDTQLPIPQALDLHRLVGTVRKTLFTENHASVLQTYLSIATSYLLISNTTESARYFYKSTKISRTVLSDIHPLTLECANHFLKITESLTTSSRTEIMTYREEILMILITAYERQYGSTSELVIQTRKVLIELYASLNEQDKVMEIYRLIQEATIQQYGRNSHQAQDVQGHLNVTLGRGKGEHSIDGYKESWFHDDDEEDLVEVFDIASIVAYLRRAESHVAKKEYSLAERTYVELWMEVSSKCRTVQSVEWHEKNIEIATKYSQFLKTQTRTSESTAILTTVWQQYEHHQLSFAESIVSRLTVVAKEMKSVGSYTQALSIFKFASSYYKNVRHEETSISREINEQLSETSTELVKQSLSSSSSVTETTTTVSESVFQEVFFSIIQSSKTVDSSTIALAKKLTIQYIEKKNYTAAISVIHATLQRTWSTFLASSIHDVTMTSTFTQDSIELVERLAECYSHLRQLEKVEDTYSRFFRAVLVTEKVDKSIFERARTLLITFYDKHGYADKSISIFQEILVAYRARFGYSSETIQTLYILARRCQSHPRNHPYWVDYFLQIITALNKDSDVCHKDALDAIVVVTTTYWEDRRYAEAVSIYRVLWNTFVRQTKEHKVFSEVKFVQTLYERYYQCLEETKASWSELHKVTKEYRETVTAVFGAESTIAVEATLSLAQVSQRSEEHASEAIALYEAVSKSSKTTSTKITHSEINQALSSLYVRQLKSSSSSSMKAETVQRAISMTESQFQESTRAYGYSHESSLTSLRELSTLYSRQQKTDLAIKQITTAVSEIITKETSSQKQIESAASIASTFRSIEQTSTAYSLVEELHRQICAKESRYASKWSFDLTKSNRTALAFLASLQYNIRKDLSITFAEIMADLTMEYIYFEQFRLTLQNNESLTNILLAAAPLRWFLRRNGQQVMINVVEDQAVGLFVKRDAQDLNTLSKESPRIFIIGILDQLGNGRNKDFNRSVILAGNDSVTKLTKAKKFPEAYDIANLGFLYASKHDGYNGPRAISLGFKLASLLVGRDGEKCPDAALRKKMLELSNRIVKKILEICKRLDINFAQVQLYELSHLSALLGEQEDYETLEWLLTTLWQTRDAQRSWPASVLLNLGRRLICARYLAGRPVKAIRLAEDIAYNMRRAHGPRAPVTIETYELLAQLYTSTGQSYQAQAAKGEKTGGLAQDYFKKAVGVHEDVLRLVVHEQGSGKDSDDDELDTAAALLAREGVSVKSSPNTPTTTSSMDSATIDRSATALKHLHLLKLAYQRLGGWPKPYDEYERLNAQIFRAFGAEEQWKGVAGTEKWDAKAFGGGKAESQDGGFRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMM5</accession>
    <name>A0A9W9CMM5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Proteasome component M29</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ECM29</name>
        <name evidence="7" type="ORF">N0V83_005124</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="16066at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024372">
        <property type="entry name" value="Ecm29_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055443">
        <property type="entry name" value="HEAT_ECM29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346:SF19">
        <property type="entry name" value="PROTEASOME ADAPTER AND SCAFFOLD PROTEIN ECM29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR GCN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13001">
        <property type="entry name" value="ECM29_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24492">
        <property type="entry name" value="HEAT_ECM29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Proteasome component Ecm29 N-terminal" evidence="5">
        <location>
            <begin position="15"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome adapter and scaffold protein ECM29 HEAT-repeat" evidence="6">
        <location>
            <begin position="1319"/>
            <end position="1480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4370603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1686" mass="187010" checksum="C903DB217ABAC5B0" modified="2023-11-08" version="1">MASEQTPEAKELALVGKVEMRIALASSEKKLEDLLKTYLAPLLLKLGSEHVAVRNKVISICQHINTRTKSQDIKLPVAALLKQYKENADVALIRHFDILYIQQGISRLSVSERLELLPILLQGIANDYDKSVQHASQLFHLILRLLVHFKLPLRGSKDDDELRSTLGLKDEDAAFLSKWFGKLLLLNIVRQGHPESGASLSCPGLSVEEYKFLTLQGKPDAWDPSSDSGLNLTESKALVTRFLASGMFNEDEKFLPALFASADTNSRISEVGEDTLKRVIISKDLENAKVVESLFELYFGSGTSDGQLPVKTPLRIRILNMLSRSVKCTAYPRQIARLVEEGLLSPELNATNKTAGREASKFRSAIFSLVNFVARRGDSKDLSVVAESLVGNLRAFLQDQGWPTPDRDQDMELRGYGYETIGLLAKASPEKILIEPTLDLLEWLFRSLREDSAGKDVAVSIEEALSSVLGAFAKPFDTSVMPRFRQILLRYASVDQVNTPASDRFTRSTRYVATRFANRCLPYDDVLARWIDILAISGGATERHEVVEEGRRGLDPYWFRMSNTPPGEREKSKLAFPDFDRLVSFIFVQQGEDEDAMDLDESGDVLTQVQHIYRQRAEVLPTVITFCSAVAMQSALDEKKLGDDIPEDWGRKLDTQMTTDLRARQAFRAFAANESHARSLALILRASFDRLTRDDVSDIGDIGTVLTRLLSLLPQQQWLLAHLVQDFRALEPSILSNNGGRRLAAAHAYGLLASHDDCDQESLRKSQALFFDKLSTWKSAVGAEINQISGVILALGYYFSRAFWRSGASSSVIGDDHPIQALLSSLVEILKESRDATLKGAAYSCIDQLSLFHVITPSLIGKYAKVEDVAQQLYDAAKTGTIGAILALGHFSMITEEAEGDTEDATDYKIIGDKLYELHEVRQPEVHFSVGEALSCFANGWDSKALTAELDILHPDQAQDPWDAPLQTPSGPKREKTLGRVLEKTLKGCLQTKPSLKKASVIWLLCLLQYCGHKPEMQGYLGQCQLAFKNCLSDRDEVVQEAASRGLGLVYEKGDRQLKDDLVRDLVGSFSDNKSKMAGTVSEDTQLFEPGALPTGDGSITTYKDILSLAAEVGDSSLVYRFMSMASNNSIWSSRAAFGRFGLSNIFSDSSVDGYLAQNPKLYPKLYRYRFDPNTNVQRSMNDIWNALVKDSSATIDKHFDAIMDDLLVSILTKEWRGRSIEKYEKYLSAVWDKTFKVLDDIKETVRVAAASLARVLTAILTRSLEAGDASIKSANAQLTRVLPFLFSTSGLESSAEEVRLFSVHTLLQIVKKSNANTLNPHVPELVERLLGLLSSLEPEAVNYLHLNASKYNLTEQKIDDMRLQSVRSSPLTESIERCLDLADASTMKALVPRIEAAMKNAVGLPSKVGCSRILVTLATRHRFLFNPYADSFLKLIQKQIHDRNETVSSSYAAAAGYLARLASDKQLVSTITFITALYFGSSSSSETSDNSDKNRLLAADTTRAISLHATDRFTSFAASLLPFIFLAKHDSDEQVQKLFAETWDDHVAGPRTVSLYLTEILALADTHLEDRQWAIKHTAAKTVADAILQITNAVGSENIENVGVATAIWPVLDKALGGKSWEGKEVVLEAFVRFVERSEGAFWKGDEGVARALEKVASREAKRQEGKNEEVVRGVLEKLRGRLGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMS0</accession>
    <name>A0A9W9CMS0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycoside hydrolase family 43 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_004737</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glycosyl hydrolase 43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMS0"/>
    <dbReference type="OrthoDB" id="5211809at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18619">
        <property type="entry name" value="GH43_CoXyl43_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006710">
        <property type="entry name" value="Glyco_hydro_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052176">
        <property type="entry name" value="Glycosyl_Hydrlase_43_Enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43772">
        <property type="entry name" value="ENDO-1,4-BETA-XYLANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43772:SF2">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_2G04480)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04616">
        <property type="entry name" value="Glyco_hydro_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="site" description="Important for catalytic activity, responsible for pKa modulation of the active site Glu and correct orientation of both the proton donor and substrate" evidence="5">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606710-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4371518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="36915" checksum="28F5908F1446F10A" modified="2023-11-08" version="1">MADKEPIVTHVFTADPSAHVFNNKLYIYPSHDRETDIKDNDNGDQYDMNDYHVFSMDEVGGPVTDHGVVLAQSDIPWVQKQLWAPDAAAKNGKYYLFFPARDQDGIFRVGVAEGDKPEGPFKADPEPIKGSFSIDPASFVDEDGEAYLYFGGIWGGQLQCWKTGKFVREDYETMEASEGNALTAKVAKLSDDMNSFTSEVKDVVIVDDAGEPIKASDHDRRFFEAAWVHKYNGKYYFSYSTGDTHYLCYAIGDNPYGPFTYGGRIFEPVIGWTTHHSIAEFKGKWYLFYHDSSLSKGVNHLRCVKIREIVYDEQGRIKLAEEQPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMZ5</accession>
    <name>A0A9W9CMZ5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MFS transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004497</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMZ5"/>
    <dbReference type="OrthoDB" id="3797192at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF234">
        <property type="entry name" value="MFS-TYPE TRANSPORTER DBAD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="313"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="409"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="437"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="468" mass="51090" checksum="59CAF76BDB76B8CF" modified="2023-11-08" version="1">MADNWRHTGSGTALHAGQVFVNPAIRKHIDSQEGVTGWQNKRPHEADPGQRAFNVVEAGIATSASARRIRTYYAQALILFNVYGVPLSFGPYLEYYHTTLLPTTTLWSLSLIVAIQMLCIFAAPYPIWILYHRWPKQRRIMVFTSLLAVVGAHGSLIFCKTHLEIMLLQGLLMGCGLGALFTLGTLFLGSHYKFNLPMASWISASGGFAGALVHTATAWQLLRRDQWKAANAVSLGVAIVTLLAAFFLAKHSERGNVQLDVRAPQSLRAIFLEKGALWFVIGYILVFFGVFIWPIYVVLILSHAPAFLWPDAGAWTLLAMLGTAFFASPVCANVHFRRHLPPVASFSAACVFAGVSVITPAWVPQFWFSVGCGAAYGVALGAILTLHIKVVAVFHPLHGVWHPDMPVRGALMMALGGIAAAAGFVTTAVLLEKKKNGSKIVAILAIVCLMLGGMMIAGVRWWRRKGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN05</accession>
    <name>A0A9W9CN05_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">N-alpha-acetyltransferase 40</fullName>
            <ecNumber evidence="4">2.3.1.257</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_005261</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-seryl-[histone H2A] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-seryl-[histone H2A] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50600"/>
            <dbReference type="Rhea" id="RHEA-COMP:12742"/>
            <dbReference type="Rhea" id="RHEA-COMP:12744"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64738"/>
            <dbReference type="ChEBI" id="CHEBI:83690"/>
            <dbReference type="EC" id="2.3.1.257"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-terminal L-seryl-[histone H4] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-seryl-[histone H4] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50596"/>
            <dbReference type="Rhea" id="RHEA-COMP:12740"/>
            <dbReference type="Rhea" id="RHEA-COMP:12743"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64738"/>
            <dbReference type="ChEBI" id="CHEBI:83690"/>
            <dbReference type="EC" id="2.3.1.257"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. NAA40 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.257" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN05"/>
    <dbReference type="OrthoDB" id="424551at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043998">
        <property type="term" value="F:histone H2A acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010485">
        <property type="term" value="F:histone H4 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990189">
        <property type="term" value="F:protein N-terminal-serine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039949">
        <property type="entry name" value="NAA40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20531">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20531:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="13">
        <location>
            <begin position="209"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="149"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="217"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="154"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="232"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4370740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="371" mass="41732" checksum="AEA91D416A697F2B" modified="2023-11-08" version="1">MPRTRYQQEIVTWKQQFKKEFNRNPTYIPSIPDPRQITEEPTHTYLDADDMPHIVDPRAREIQKLFEEDITGLRAYEAPQLVCPLKFDFVRSGHDLKIKELSACLSLVESTSGRDYRASRIGWDPNNKKAEMLDKNMIYLLIRQGDVGGPVEQVPEDDLQEGDVDMDLPPAQQGDDDGLVEQAPQNELQAGEAHMNSSPSQQLNALEQLIQGYASDGDAEDTANAESTLKPDSPPSSPVPFIGPQPPTLVEWPDPGNNGRMFGFVSFMFTYDDPPHDDREVVYLYEIHLHHQLRGRGLGSNLIKFVERAAAHCGISKVMLTVFVANATAMALYEKLGYTKDECSPEGRVTRKKTFPPEYLILSKTIPCIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN89</accession>
    <name>A0A9W9CN89_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_003160</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN89"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11387">
        <property type="entry name" value="bHLHzip_USF_MITF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051732">
        <property type="entry name" value="USF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46117">
        <property type="entry name" value="FI24210P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46117:SF3">
        <property type="entry name" value="FI24210P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="332"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="312"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="488"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="374"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="523"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="548"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4372869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="557" mass="61558" checksum="CB850FECBC5257AE" modified="2023-11-08" version="1">MYYETILTVPQADSLFTPPSSYSPQKPFARLDLGLDSPSDDVLDADIFGMTGTGSPQPYIKEEVDDMSSYTNNNNGRFMGHNGFDMNQQFSNPQYSSTHDNVNGINPSDLTMNGSMMGNFGSTSYIQGAAGIADDELADSLGNFDQQQGFNGFAQENMNQQDVYHSHSTNTNMNQIYSNTPDDLPIHSPFVHPSNFDFNQYQSGPQRTGFPGSIQNGSMRQRITMSRNGSDSRAPMSPKTPAMAGLHLGTPDSGNFTTQPIMTNMHRHQKSVSGQWEGTPGSQHSWIDSPTASPHTTAMHHQQISEVLHSGKHNSLPTKVDIGQTQDAKKRRRRESHNLVERRRRDNINERIHDLSRLVPQHRLEDEKIRKHINNNGPMSPTMATSGMSPPQATSLLAGGSGRRAAGNITQGLPIEDKDKGPNKGDILNGAVSWTRDLMWMLYKKIQEVDDLSARLQQATGEEWSTEMTEDEKRMKTEIIDAIEKNGTGTFRYSRGPGSGLRVPKHTNLAGEPLNGISPQSLSPGMQSTGSGSGSNQQQYWSNQSLKEEDEYGMDMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNN7</accession>
    <name>A0A9W9CNN7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ER membrane protein complex subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_003813</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNN7"/>
    <dbReference type="OrthoDB" id="16510at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008504">
        <property type="entry name" value="Emc6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029008">
        <property type="entry name" value="EMC6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20994">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20994:SF0">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07019">
        <property type="entry name" value="EMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4372040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="114" mass="12269" checksum="C07E29D74E331421" modified="2023-11-08" version="1">MAASALNEERELAINPIVASSVQHNTQVISNIRNLTASLFGVAAGTLGLESYTGFIFYLIASLTVSALLFALKTDGKPAEYFHRPLGDLWLGDVFGGLSGFVLTWTLFYGLVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPC7</accession>
    <name>A0A9W9CPC7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycoside hydrolase family 125 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003508</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPC7"/>
    <dbReference type="OrthoDB" id="7771656at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008313">
        <property type="entry name" value="GH125"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31047:SF1">
        <property type="entry name" value="DUF1237 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31047">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 157 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06824">
        <property type="entry name" value="Glyco_hydro_125"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028846">
        <property type="entry name" value="UCP028846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01149">
        <property type="entry name" value="DUF1237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040833382" description="Glycoside hydrolase family 125 protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="512" mass="56896" checksum="E5FF7AE8018BD240" modified="2023-11-08" version="1">MRGLKLFSALNVPLLATAQCPEYIDYSNQTHEPLSSGKYKLSYQRPSEECRTFKSQGLEDTITRMESVIKDPDLYRLFQNAYPNTLDTAIKWKGYAADDAEEELTFIITGDINAMWLRDSSNQMQSYLPLLNASSDPNSIASLYRGVINLQARYLLTSPYCNSFQAPVESNIEPAPNDAGSGDTVFPSYDNQTVFECKYELDSLAAFLEISVDYYNATGDSEFFKKFQWVETVETILDVAQNMTTPTYGPDGAVLQSPYTYVRYTNRATETFSNDGIGNPVANGTGLIRSGFRPSDDSTIYQFYIPSNMMLSSYLARASEIMSQLDTSNTVGLAQRMSDLSDSLHDAIETQGTVDHPTYGKIYAFEVDGFGGTNIMDDANIPGLLGAPFFGYSVNSDTYANTRELILSAANPYFMRGPVISAIGGPHQGPGMAWPMASIVRILTTDDDDEIINELGQIVSSTDGLGLIHESINSFDQHKWTRQWFSWANGLFGQMILDLEDRKPEILATSFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQC8</accession>
    <name>A0A9W9CQC8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Replication factor A protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001398</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the replication factor A protein 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQC8"/>
    <dbReference type="OrthoDB" id="188186at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04479">
        <property type="entry name" value="RPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000271">
        <property type="entry name" value="Similar to ssDNA binding protein Ssb3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013970">
        <property type="entry name" value="Rfa2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15114:SF1">
        <property type="entry name" value="REPLICATION PROTEIN A 14 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15114">
        <property type="entry name" value="REPLICATION PROTEIN A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08661">
        <property type="entry name" value="Rep_fac-A_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4376117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="11664" checksum="7E591802C2DD9D62" modified="2023-11-08" version="1">MDHPQTPRILAPHLSNFQHRIVRVLGKVVQLRGDSAVIDAGGNIDVILNRDSHLAPGHAVEIIGKVDGNLNIKVQAATDFGTNVDFNAANAVVEATHRYKEIFYDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQL9</accession>
    <name>A0A9W9CQL9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_001670</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQL9"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056002">
        <property type="entry name" value="DUF7580"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF11">
        <property type="entry name" value="CEREVISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24476">
        <property type="entry name" value="DUF7580"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040946794" description="Peptidase S8/S53 domain-containing protein" evidence="6">
        <location>
            <begin position="26"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7580" evidence="8">
        <location>
            <begin position="4"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="7">
        <location>
            <begin position="232"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4376387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="507" mass="57506" checksum="08768ADD97DDBC28" modified="2023-11-08" version="1">MQIHTHPAILALGIIFLEIITGTRFKRTREDTPWEQCNKDNSQALQLLNQLEKKDRHNREKRISSGMNRAIRACLKLEPPPNFPSNRLSEEGPIRHYILQCIVRPLAIELQEGYKVQLDALHKALIPGHEGDDPDGLDDVKSFSRRSTASIKENDHNDRKDISHSSVKTQVKFKTDIATSSTERREACSYRNREELVDEHKVKAATTWFEWHDDASSRITEIRDSTKSNARRVKIAILDSGIELSQDTKDMYDFEPKIQYRSWVDEDETWKDEVGHGTHLAILLRKIAPNAMIHVERVFKKKPTTSSTNKIADAIRAAVEAGVDIIVMSFGFGEEHKDLYDAIQEAAYKNVLVFAAASNDGKNRPDGVAWPAKDNNVICVHSGDGNGARSTFTPSPSDNMRIMVLGECVKSACPPHLKCAADHKLMSGTSCAAPIAAGIAALILDYARGFLDEHEWNRLRRTTAMLRMFKRMKDEHSTDGYWWIKHWQWFDSSRTVGWIQGEIRGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQP8</accession>
    <name>A0A9W9CQP8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WIBG Mago-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002182</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQP8"/>
    <dbReference type="OrthoDB" id="21625at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903259">
        <property type="term" value="P:exon-exon junction complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039333">
        <property type="entry name" value="PYM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015362">
        <property type="entry name" value="WIBG_mago-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036348">
        <property type="entry name" value="WIBG_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22959:SF0">
        <property type="entry name" value="PARTNER OF Y14 AND MAGO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22959">
        <property type="entry name" value="PYM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09282">
        <property type="entry name" value="Mago-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01273">
        <property type="entry name" value="Mago-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101931">
        <property type="entry name" value="Pym (Within the bgcn gene intron protein, WIBG), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WIBG Mago-binding" evidence="2">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="196" mass="21506" checksum="78F1FE3B68BC11DC" modified="2023-11-08" version="1">MPTVEAPAISKAGITTSATGERHIPSSVRSDGSLRKEIRVRPGYRPPEDVEVYKNRTAEGFRNRGKAGIPGADGLKGEDDATKASAAANKNAKRREARKKAAAAASDGKEDDKVAESKDDTKATEPEKVVDPEAEKEKEAKKLTKKLRQARELKDKKEKGDKLLPEQFEKVIKINELIRQLETLGFDAQGEKKVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQR5</accession>
    <name>A0A9W9CQR5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CorA metal ion transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MNR2_2</name>
        <name evidence="7" type="ORF">N0V83_002243</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQR5"/>
    <dbReference type="OrthoDB" id="29879at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010961">
        <property type="term" value="P:intracellular magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.340:FF:000008">
        <property type="entry name" value="CorA family metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21535">
        <property type="entry name" value="MAGNESIUM AND COBALT TRANSPORT PROTEIN/MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21535:SF51">
        <property type="entry name" value="MANGANESE RESISTANCE PROTEIN MNR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4375159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="74" mass="8181" checksum="C9DA6E2D2299660F" modified="2023-11-08" version="1">MNERQEKTSDILGKLTVLGTIVLPMNVVTGIWGMNCLVPGQDIESLTWFWCITGGLVGFGLLCYFIAKRVYGIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXW3</accession>
    <name>A0A9W8XXW3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010789</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXW3"/>
    <dbReference type="OrthoDB" id="3539644at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="470" mass="50558" checksum="CF68E291D818C0F0" modified="2023-11-08" version="1">MGANRELRVDAPPSSAQPVGTGISSPPASAQPLDGDESSHPEGFIDDDSDTPHLVAVPWPTQLATPVRPPQSEEPEQAFPIRWPSRCGREYNVKTEEYDFGTSKDLNIQEAVHQLDGHYKRVAGWIHVTQGPAEQVSGTIRAKFSYAVSASVNVDSIQHSTTATGLMIGDPSFPDGFDGVRSGSACLGMSVVVYVGNGATLETLNVASTHMGMQIHNGVNLTVTKSTSISLTTGTLDSASLYSRQTHLKTISGSISGKYSLLDLVSVKTKSGSVNINIEPKEATKGDTNPAIFMVDAVSSSVRVDFNRKHIPERDYQNYINTTVGSVDGTFIHGSKTKIASVAGLVTADLLPFRSGDYISEIYTHTHSGQTNLTVRTPYKAKHVPMTKLTSTHKSTSGGLDITYPDEWTGYIDGTSLNGALHLHGKDLELVTENNDPGNNHVEAKKGYEFASMMTFETVSGEYEIKIGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY79</accession>
    <name>A0A9W8XY79_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF862-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_010637</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY79"/>
    <dbReference type="OrthoDB" id="21221at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070646">
        <property type="term" value="P:protein modification by small protein removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013535">
        <property type="entry name" value="PUL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF7">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08324">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51396">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="5">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="PUL" evidence="4">
        <location>
            <begin position="302"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4362543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="602" mass="66092" checksum="BA0D10317301BEDE" modified="2023-11-08" version="1">MDVQLYVYDLSQGLARSLSRQLVGTQIDAVYHTSIVLDGIEYYFGQGVQTCRAGATHHGRPMEVIKLGSTQLPLEVILEYLESLKEVYTPESYDLFLHNCNNFSNDFSMFLVGKGIPDHITSLPQTVLNTPFGQMLRPQIDAAMRPITQAPTPQPVTPARPQATSTGVMPAAISTGAGNALEANTGRVSNVTMIRDVDRLLDLAKDRCAIIFFTSSTCAPCKLVYQPYDDLAAEAGTKCVFIKIDFSHADRSINVRFPHVRATPTFITFVKGEKRDQWSGADPRQLRSNVEILLNAAFPTHPHLEKSTPYLLKQSQRPITYPKVPPLEKLVVKMGDAGKDPTISSIVSFINAREKSGAMEAPLPKLPQFAEFLKKSTSVLPPELLFTALDLLRITLTDIRASGFFAEEHKGATGTPATVHHLLSHVDQLGDSAPYPLRLTTLHLACNLFSSPLFIPHLLSPPLSSTLISILTTALLDEKHPALKASALSLAMNLASCNHHIRMKKHSTNASHALAGESELAESEQVEILASLLENLSLEEEWSDNKKMGLICVGWLVYAADMEGELRDLWKVMDAAGSVGKIQAKAAEDRLLVREVKSLLET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYN4</accession>
    <name>A0A9W8XYN4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dihydroorotate dehydrogenase (quinone), mitochondrial</fullName>
            <ecNumber evidence="5">1.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Dihydroorotate oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">URA9</name>
        <name evidence="15" type="ORF">N0V83_010200</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(S)-dihydroorotate + a quinone = orotate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:30187"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.2" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYN4"/>
    <dbReference type="OrthoDB" id="14784at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106430">
        <property type="term" value="F:dihydroorotate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009220">
        <property type="term" value="P:pyrimidine ribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04738">
        <property type="entry name" value="DHOD_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000242">
        <property type="entry name" value="Dihydroorotate reductase PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050074">
        <property type="entry name" value="DHO_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005719">
        <property type="entry name" value="Dihydroorotate_DH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109:SF4">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00911">
        <property type="entry name" value="DHODEHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroorotate dehydrogenase catalytic" evidence="14">
        <location>
            <begin position="24"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Dihydroorotate dehydrogenase catalytic" evidence="14">
        <location>
            <begin position="399"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="315"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="342"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="365"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4363080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="456" mass="48314" checksum="8A5934FA88DE8F8D" modified="2023-11-08" version="1">MLKVLHSFGMNPRERGAADSAGDLAVEVFGHRLSNPIGTSAGVDKGAEVPTPLFELGAGIVEVGAITLLPQEGNPKPRVFRLPSQNALINRYGFNSEGAELVATRLRQRVREFAYHAGLGLDEEAERKVLNGEAGVPPGSLLPGRLMAVQVAKNKTTSESDIEAVVRDYATAVGYVGKYADILVVNVSSPNTPGLRTLQNVEPLTRLLSGVVQAVKKIDRKTKPAIMVKVSPDEDSVEQISGICEAVWDSGVDGVIVGNTTKKRPDPLPKGYLLPDSEAKILLEQGGYSGPQMFERTLALVSKYRKALDEGPKRIALPEKPVAGHATGDEPTSSILEKIEPSSSTSSSPGSSKTTTTVDLDPAATLSSNVDDLSESAPTLTLQSTPNTAPAPSGPRKVLFATGGITNGRQAREILDAGASVAQVYTAMIYGGAGTITRIKQEMRDEAKKQTTLTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYW2</accession>
    <name>A0A9W8XYW2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009716</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYW2"/>
    <dbReference type="OrthoDB" id="566138at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005127">
        <property type="entry name" value="PRK06565.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42678">
        <property type="entry name" value="AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42678:SF11">
        <property type="entry name" value="AMIDASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="1">
        <location>
            <begin position="33"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="681" mass="74484" checksum="D402DCE7707E3D52" modified="2023-11-08" version="1">MANSNEIDFNIVEASIEDHRIALNSGRITSIDLVISYLNRIAKYDTISGLNAFTVFNDQVFDEARASDARRAQGQPPRPLDGIPYTIKDSYKYTGLSVTNGSPALKSLMSSEDSAIAKKLRDAGAVLIGKTNMPPMAAGGMQRGMYGRAESPYNSKYLTGAFSSGSSTGAATAIASSMAAFGMGSETVSSGRSPASNNALVCYTPSRGILSCRGLWPLYVTCDVPVPYARSMSDMLEILNVIATPDEESKGDFWREQTIVPIPKVQHTDYKALQEQDTLKGKRIAVPKMYVGQQDSDPHAKHPFVSPAVIQLWQQTAKDLEALGAELIYTDFPLVTRYENDSLSGEANNVLNAPSDWNQVERGILIAKAWNDFLEQNGDADISSLSDIQDPTMLFPKPKDYKPDTFLEVRNWINYAGLPDLLAQVKDTSIYDLPGMEQAVKALEAQRKRDFEGWMDEMQVDVIAFPAQGDVGLADLEFNLESVKHSLQNGVKYSNGNRAIRHLGVPTVSVPMGVMSGKGMPVNLTFAGKAYEDTKLLEYAYAFEQGTKRRISPPLTPLLDTDSVAARGLPQRGDGRRLDLCAGVRSSKQADGSLLLHFGGTISVDYEDTTLQIYLDGTEAYHGKNSTETWEVELKYTPERPVCLGWDLKPLSPRKVMAIVVARGDGGAVEAKLLWAPVESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYZ6</accession>
    <name>A0A9W8XYZ6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYZ6"/>
    <dbReference type="OrthoDB" id="410198at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052523">
        <property type="entry name" value="Trichothecene_AcTrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791">
        <property type="entry name" value="GNAT FAMILY ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791:SF5">
        <property type="entry name" value="HYPOTHETICAL ACETYLTRANSFERASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13508">
        <property type="entry name" value="Acetyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="21"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="210" mass="24107" checksum="21E166629A98D35B" modified="2023-11-08" version="1">MSDRYLVQEAKTRQELDAVMDVIWAANYTPYEPFAQIFFPVLGFTTAHREAAIAESKERFWNQHQADRSSHWFYAFDTVTGRAVGCAQWVISESNPFAAALNWMAVHPSHRFQGIGSLLMNVGILRADELNLECWMEASSMGKPLYEKFKFQSLLKIAFDNEKLGASDEWRKCAHEMTPPPIFAMWRPKQGRRTSDLGGVRLPWELGTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZB9</accession>
    <name>A0A9W8XZB9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Purine permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_010263</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZB9"/>
    <dbReference type="OrthoDB" id="1641903at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042907">
        <property type="term" value="F:xanthine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006042">
        <property type="entry name" value="Xan_ur_permease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00801">
        <property type="entry name" value="ncs2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42810">
        <property type="entry name" value="PURINE PERMEASE C1399.01C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42810:SF2">
        <property type="entry name" value="PURINE PERMEASE C1399.01C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01116">
        <property type="entry name" value="XANTH_URACIL_PERMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="263"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="333"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="420"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="448"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="480"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="514"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="570"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="576"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4363143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="595" mass="63727" checksum="7C1E944FCFA3CC1B" modified="2023-11-08" version="1">MADRIGPDHTPKRSIGEKARRIVRAFTTKDGLIGTYDYGFLFRPNIPFMKKERRAAPFFGLNDRMPVFLALLLGFQHALAMLAGIITPPIILSQAANLTAEQQRYLVSTSLVVCGILSSIQITRFHIWKTPYYVGTGLISVVGTSFATIPVATGALSQMYATGYCPTAADGTKLPCPDGYGAILGTAACCALLEIAMAFTKPRILKKIFPPLVTGPTVLLIGVSLVSTGFENWAGGSGDCKSRPESGIFQLCPTISAPHALPWGSAEFIGLGFLVFVTIIFCERFGAPIMKSTAVVIGLLVGCIVAAACGYFDRSSIDAAPAVSFIWVHTFKLSVYGPIVLPFLAVYMVLTMETIGDITATCDVSRLEVDGELFDSRIQGGVFADGLNGIIAALCTMTPMSTFAQNNGVIALTRCANRKAGYACCFWLIIMGIFAKFAAALVAIPSAVLGGMTTFLFSAVTVSGIRIISTMPFTRRNRFILTAGFTLGFGATMVPTWFAYVFTYDGPNRALLGFYNAIVLIMETGFAVVAFTTVILNLILPEEIEDEETPELTANEVDGAVDSEEWARIQKGQVKGSDEEARASSEVEPVAGKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZP9</accession>
    <name>A0A9W8XZP9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Translation initiation factor eIF-2 beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SUI3</name>
        <name evidence="6" type="ORF">N0V83_010943</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZP9"/>
    <dbReference type="OrthoDB" id="10255414at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor IF2/IF5" evidence="5">
        <location>
            <begin position="184"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="118"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="317"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="513"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="126"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="330"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="362"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="412"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4362002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="533" mass="57665" checksum="3A252F96655881C2" modified="2023-11-08" version="1">MADTADLPERKPRKSVAFSEGATIVDSNGDVTESKDMNGGKSSAESHASGPPTPPAEDKDVEEVTDMFADLAKKKKKKSTKKKEGEDDDAAEGDFAALKKKKKSSKKKVEDDFEAKLAAAGGDKGEGEEEEAAAAEPEVQEGDMMKGTGIWQHDATTPITYPLLLNRFFTLLHSQHPDLASSGTKSYKIPPPQCLREGNKKTIFANIAEICKRMKRTDEHVTQFLFAELGTSGSVDGSRRLVIKGRFQQKQIENVLRRYIVEYVTCKTCRSPDTDLSKGENRLNFVTCNSCGSRRSVQAIKTGFSAQIGKRRRMVADQLGQQQGQQQYYAPPPGGQSYPPPPTSPPAQQQQQQHYPPPPGAGQPAPQYAPPEQQQHFPPPGSAPPQLQQQHSGYPPEKGPLSPYGGPPSHTPQQQDPRAAAPQHPGMPQQQLSQQQIPQQPQFAGAGAGAGSAAATTADDVGTFNGGSYRVSHRDTNSILTVQLAIGCPLTAKPGKSIIQLYLNQKELAYPLEAVSPDEKPPGNTTRAPERFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZX5</accession>
    <name>A0A9W8XZX5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010719</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZX5"/>
    <dbReference type="OrthoDB" id="870at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14837">
        <property type="entry name" value="AP3_Mu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="7">
        <location>
            <begin position="200"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="163"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="346"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="457"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="484"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4361779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="575" mass="61064" checksum="AAAD390C3B5D37C2" modified="2023-11-08" version="1">MSTIEALYIFDEQNTPILEHTYIGRPPASSTLLPLYLSHPSPRPSLIYLPNTNPPTLLYSLTQDQLLFLCPCASDTEPLQVLEFLHRVADVLEDFLGSPLLAGKIENHYDVVAQLLSEMVDGGVIACTEPNALRDVVEAPNFMKSLLGGVGLPSATPSSLAPSATPFSLNARPSPRLGPTSATQTTSPVPWRRANVRHTSNEMYVDIVETLEVTLSPSGRPLSAIANGTIAFTAKVSGVPDLLLHLACPGGVDNAVSLPVFHPCVRLNRWKERPGELSFVPPDGRFVLAGYEVDLLGSAALDSFSTATTAKSLPKLNVPATVSVHPSLGPIGSDFEVRLQLNPRFTGKSASPHPSTMAAKGGLGRGLGGAPFSSSGTTAAPAIEDMVIHIPLPSSVRNVTDLRVLRGTGDAAYAPGDQYIEWRISSREIGILMSQDRGGGIGATATLRGTVVGQDVEDESSDALDSGGPVSFSTSTYDYDDDQTTTTTTSHITTPSQSTKQQNADADSKRIKSNKVLMPSCATVSFSVKGWLPSGIKVESLNVDQKRSKGLGSGVTPYKGVKYLCVSRRGVECRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZZ2</accession>
    <name>A0A9W8XZZ2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">inositol-3-phosphate synthase</fullName>
            <ecNumber evidence="6">5.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">INO1</name>
        <name evidence="16" type="ORF">N0V83_010395</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucose 6-phosphate = 1D-myo-inositol 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:10716"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="5.5.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the myo-inositol 1-phosphate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.5.1.4" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZZ2"/>
    <dbReference type="OrthoDB" id="2887at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004512">
        <property type="term" value="F:inositol-3-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000069">
        <property type="entry name" value="Inositol-3-phosphate synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000204">
        <property type="entry name" value="Inositol-3-phosphate synthase 1-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2360.10">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002587">
        <property type="entry name" value="Myo-inos-1-P_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013021">
        <property type="entry name" value="Myo-inos-1-P_Synthase_GAPDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11510">
        <property type="entry name" value="MYO-INOSITOL-1 PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01658">
        <property type="entry name" value="Inos-1-P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07994">
        <property type="entry name" value="NAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015578">
        <property type="entry name" value="Myoinos-ppht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0398">Inositol biosynthesis</keyword>
    <keyword evidence="13 16" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myo-inositol-1-phosphate synthase GAPDH-like" evidence="15">
        <location>
            <begin position="331"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4362302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="537" mass="58771" checksum="A460967E99E61C20" modified="2023-11-08" version="1">MAPHAEDSVGQANGYDVKTATPKDLFVVNSPNVEYTDETIKSKYTYRTTDVTQNADGKYVAIPKETLYNFKVDRKIPNVGVMLVGWGGNNGTTVTAGIIANKRNLVWETKEGKRGANYYGSVIMSSTTKLGVDSKTGKEIHIPFHDLLPMVHPNDLVVGGWDISGMNLADAMDRAKVLSPSLKSLVRKEMGAMKPLPSIYYPDFIAANQEERADNILEGSKASMAHVEQIRKDIRDFKAANNLDKVIVQWTANTERYADILEGVNDTADNLLKAIEAGHEEVAPSTVFAIASILEGAPFINGSPQNTFVPGAIEFAEKHNAFIGGDDFKSGQTKMKSALVDFLINAGIKLTSIASYNHLGNNDGMNLSSQKQFRSKEISKSNVVDDMVAANTVLYKEGEHPDHCVVIKYMPAVGDDKRALDEYYAEIFLDGHQTISLFNVCEDSLLASPLIIDLVLVAEMMTRVQWKAHSAEASDAKYSGFHSVLSVLSYMLKAPLTPAGTPVVNALAKQRAALTNIFRACVGLEPENDMTLEHKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZZ3</accession>
    <name>A0A9W8XZZ3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZZ3"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="70" mass="8352" checksum="D12AB2D6D5993074" modified="2023-11-08" version="1">MPQLQQGKKEGQYWGRATWGSKQSKAKKKKKKKKKKKKKKKKKKEEEEEEEGGEGRRRGDKCSERKYIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y029</accession>
    <name>A0A9W8Y029_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Aprataxin-like protein</fullName>
            <ecNumber evidence="4">3.6.1.71</ecNumber>
            <ecNumber evidence="3">3.6.1.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Hit family protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">HNT3</name>
        <name evidence="22" type="ORF">N0V83_010455</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Likewise, catalyzes the release of 3'-linked guanosine (DNAppG) and inosine (DNAppI) from DNA, but has higher specific activity with 5'-linked adenosine (AppDNA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 3'-end 2'-deoxyribonucleotide-3'-diphospho-5'-guanosine-DNA + H2O = a 3'-end 2'-deoxyribonucleotide 3'-phosphate-DNA + GMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:52140"/>
            <dbReference type="Rhea" id="RHEA-COMP:13186"/>
            <dbReference type="Rhea" id="RHEA-COMP:13187"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:136419"/>
            <dbReference type="ChEBI" id="CHEBI:136420"/>
            <dbReference type="EC" id="3.6.1.72"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 5'-end adenosine-5'-diphospho-5'-2'-deoxyribonucleoside-DNA + H2O = a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:52128"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:13181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:136413"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.6.1.71"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 5'-end adenosine-5'-diphospho-5'-ribonucleoside-2'-deoxyribonucleotide-DNA + H2O = a 5'-end 5'-phospho-ribonucleoside-2'-deoxyribonucleotide-DNA + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:52132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13182"/>
            <dbReference type="Rhea" id="RHEA-COMP:13183"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136414"/>
            <dbReference type="ChEBI" id="CHEBI:136415"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.6.1.71"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.71" evidence="4"/>
    <dbReference type="EC" id="3.6.1.72" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y029"/>
    <dbReference type="OrthoDB" id="3512845at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033699">
        <property type="term" value="F:DNA 5'-adenosine monophosphate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120108">
        <property type="term" value="F:DNA-3'-diphospho-5'-guanosine diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990165">
        <property type="term" value="F:single-strand break-containing DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000012">
        <property type="term" value="P:single strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000017">
        <property type="entry name" value="Aprataxin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032566">
        <property type="entry name" value="Znf-C2HE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486:SF4">
        <property type="entry name" value="APRATAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486">
        <property type="entry name" value="APRATAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16278">
        <property type="entry name" value="zf-C2HE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="HIT" evidence="20">
        <location>
            <begin position="98"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Aprataxin C2HE/C2H2/C2HC zinc finger" evidence="21">
        <location>
            <begin position="250"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00068941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00076243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF01230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF16278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ4362362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="309" mass="35112" checksum="E6B287AF2FD8F337" modified="2023-11-08" version="1">MASTSSNSSPPQAQTHDAITAEEITGEETPNSLQKTTDPSSQKRPNAFTELMSSTKKPKPTTQQPPGVLDKAKSVVRGFDPRNGLSIYIEKPEANPEGRVVEYDDDFVVINDKYPKASVHLLLLPRKPEYYTQHPLSLLSTNPHFLSELHKRVARLKQLAASELRRQYGHLSASDAPYQQALEALMSSADLPPPTPEECAARLPPGRDWLADIVAGVHTHPSMNHLHVHVFSRDMQSPWMKHKKHYLSFNSTFLVRLEEFPLEEGSERFHPGDWPGWDMKCWRCGQNFKNKFAALKAHLEEEFEEWKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y095</accession>
    <name>A0A9W8Y095_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">60S ribosomal protein L23A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RPL23A</name>
        <name evidence="5" type="ORF">N0V83_009413</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y095"/>
    <dbReference type="OrthoDB" id="407959at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000003">
        <property type="entry name" value="60S ribosomal protein L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_uL14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006344">
        <property type="entry name" value="PRK08571.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L14/L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4363960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="141" mass="14915" checksum="AB86BB6BE979C754" modified="2023-11-08" version="1">MASKRGRGSTSGNKLKMTLGLPVGAVMNCCDNSGARNLYIISVKGIGARLNRLPAGGAGDMVMATVKKGKPELRKKVMPAVIVRQSKPWRRADGVYLYFEDNAGVIVNPKGEMKGSAITGPVAKEAAELWPRIASNSGVVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0E7</accession>
    <name>A0A9W8Y0E7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ribonuclease H</fullName>
            <ecNumber evidence="3">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_010636</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNase H family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0E7"/>
    <dbReference type="OrthoDB" id="407198at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09280">
        <property type="entry name" value="RNase_HI_eukaryote_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050092">
        <property type="entry name" value="RNase_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10642">
        <property type="entry name" value="RIBONUCLEASE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10642:SF26">
        <property type="entry name" value="RIBONUCLEASE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="9">
        <location>
            <begin position="7"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="162"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="178"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4362542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="201" mass="22432" checksum="98E79967BA0CADF1" modified="2023-11-08" version="1">MDYSKEFPGCLVINTDGSSFRNGKEDATAGVGVFFGKRDRRNVGVPLGPGKQTNQRAELMAIKIALDKADVKANVVIRSDSMYAIESLTNWHRRWKLASWKAPAGIFRENNALVETILERMQLRKHVGASTRFFWVKGHAGDPGNEAADKLAVRGSKRAWNQRKFGDRRSRKVKGRARPAPQEAAPQPAKKQVRFAEVDGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0H4</accession>
    <name>A0A9W8Y0H4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Acyl-CoA desaturase</fullName>
            <ecNumber evidence="16">1.14.19.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">OLE1_2</name>
        <name evidence="20" type="ORF">N0V83_010262</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Stearoyl-CoA desaturase that utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>octadecanoyl-CoA + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = (9Z)-octadecenoyl-CoA + 2 Fe(III)-[cytochrome b5] + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:19721"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="EC" id="1.14.19.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="16">Expected to bind 2 Fe(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.1" evidence="16"/>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0H4"/>
    <dbReference type="OrthoDB" id="10260134at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004768">
        <property type="term" value="F:stearoyl-CoA 9-desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03505">
        <property type="entry name" value="Delta9-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000004">
        <property type="entry name" value="Acyl-CoA desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009160">
        <property type="entry name" value="Acyl-CoA_deSatase_haem/ster-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015876">
        <property type="entry name" value="Acyl-CoA_DS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001522">
        <property type="entry name" value="FADS-1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351">
        <property type="entry name" value="ACYL-COA DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351:SF31">
        <property type="entry name" value="DESATURASE 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000345">
        <property type="entry name" value="OLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00075">
        <property type="entry name" value="FACDDSATRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00476">
        <property type="entry name" value="FATTY_ACID_DESATUR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0249">Electron transport</keyword>
    <keyword evidence="15 16" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="8 16" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 16" id="KW-0349">Heme</keyword>
    <keyword evidence="12 16" id="KW-0408">Iron</keyword>
    <keyword evidence="4 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="13 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 16 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="19">
        <location>
            <begin position="353"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ4363142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="480" mass="54396" checksum="3A92CC7284D3F45E" modified="2023-11-08" version="1">MVSHQPTAGEQAIDPDYVKQPSAMASKSEPNRNPKYDPKKPHITDTPITKANWYKHVNWLNVYFIIGIPLAGCVAAFWTPLQWKTALWAVVYYFWTGLGITAGYHRLWAHKSYNASLPLSVFLALVGGGAVEGSIRWWSRDHRAHHRYTDTNKDPYSVRKGLLYSHLGWMVMKQNPKRIGRTDITDLNEDPVVVWQHKHYIKVVLFMGLIFPSAVAGLLWNDWKGGFIYAGILRIFFVQQATFCVNSLAHWLGDQPFDDRNSPRDHVITALVTLGEGYHNFHHEFPSDYRNAIEWHQYDPTKWSIWLWSKLGLASNLKQFRANEIEKGRVQQLQKKIDQKRAKLDWGVPLDQLPVIEWDDYVEQAKNGRGLIAVAGVVHDVTDFISEHPGGKTLIKSGIGKDATAMFNGGVYFHSNAAHNLLSTMRVGVIRGGCEVEIWKRAQKENKEVNMAKDDQGNPIIRAGNQVTKVAQPIVSASAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1H1</accession>
    <name>A0A9W8Y1H1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">N0V83_008640</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Mycotoxin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1H1"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902181">
        <property type="term" value="P:verruculogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11061">
        <property type="entry name" value="CYP67-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000063">
        <property type="entry name" value="Cytochrome P450 monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF237">
        <property type="entry name" value="CYTOCHROME P450 MONOOXYGENASE ATNE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4365024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="59730" checksum="48D138F92B7FF686" modified="2023-11-08" version="1">MEFLIGLVKSLLASPWLLLLSIFGSLFFYISLVIIYRVTLHPLARYPGPFLAKITDIYLAYYAYKGSRHLAFQRAHLQYGPYVRLGPNLLSVNTATGLKSIYGFRSNVRKASFYEAFPSTPKAVSVHSAIDKMQHARKRRVMSHAFSDAAIKSLEKYILANVRVGCELLRRKSNGGISENGADDKGWNDQWNAAHWCEWLVFDIMGDLVFGKAFGMLESPVNRFATELVGNAAHRHLICGTHLTIHNWHLDKLLFRKIAGQRAQYMQYSKRQAMGRTKLGVDVDRKDFFYYLLNAKDPETGKGFSMDELWGESNLLIIAGSDTTSTAMAGTFFYLANNAAALQKVRKEIREAFIDVEEIVTGPKLSGCAFLRACIDETMRMTPPVAGALPRQVLPGGLDIDGHHIPAGVDVGVPIYSIHHNPDYFPRPFDYLPERWLSDVSANPLHDRLPDAQSAFNPFSIGPRGCIGKGLAYVELTVTIARVLFLYDLRLAPGTTLGAGSKDLEVGRTRATEYQIQDVFASKKDGPILQFRAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1Z5</accession>
    <name>A0A9W8Y1Z5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="5 17">4.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">HEM2</name>
        <name evidence="20" type="ORF">N0V83_008711</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 17">
            <text>2 5-aminolevulinate = porphobilinogen + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 17">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the ALAD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="5 17"/>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1Z5"/>
    <dbReference type="OrthoDB" id="1530at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04824">
        <property type="entry name" value="eu_ALAD_PBGS_cysteine_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000048">
        <property type="entry name" value="Delta-aminolevulinic acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006762">
        <property type="entry name" value="PRK09283.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458:SF0">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="10 17 20" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 17" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="14">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="14">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ4365094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="376" mass="40786" checksum="0D5ADE123ED2AFF2" modified="2023-11-08" version="1">MSFSSLVSDIAYRNSERPERPDLRSQASEARSTRSYASTAATSVSIAGDIKSQLHGGYSHPLSRAWQAERQLTKSMLIYPLFISDQPDEETLIPSLPNQHRRGLNKIVPYLTPLVAKGLKSVILFGVPIAPNAKDALGTNADDPKGPVIRAIRLLRRAFPDLFIVADVCLCEYTSHGHCGILRDDGSLNNALSVERISDVAISYAQAGAHCVAPSDMNDGRIRAIKLKLIEAGIAHQIVLMSYSAKFSGCLYGPFRDAAGSCPSFGDRRCYQLPPGGRGLARRAITRDINEGADIIMVKPASQYLDIISDAKEIGKDMPVAAYQVSGEYAMIHAAAAAGVFDLRQMAEEATQGILRAGASIVVSYFTPEFLDWLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-06-18" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1Z7</accession>
    <name>A0A9W8Y1Z7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">DNA polymerase epsilon catalytic subunit</fullName>
            <ecNumber evidence="19">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">POL2</name>
        <name evidence="22" type="ORF">N0V83_008435</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17 19">DNA polymerase II participates in chromosomal DNA replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="18">Heterotetramer. Consists of 4 subunits: POL2, DPB2, DPB3 and DPB4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 19">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="19"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10060449at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05779">
        <property type="entry name" value="DNA_polB_epsilon_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05535">
        <property type="entry name" value="POLBc_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000002">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000015">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1600.10:FF:000006">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013697">
        <property type="entry name" value="DNA_pol_e_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029703">
        <property type="entry name" value="POL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055191">
        <property type="entry name" value="POL2_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054475">
        <property type="entry name" value="Znf-DPOE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670:SF0">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08490">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22634">
        <property type="entry name" value="POL2_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22912">
        <property type="entry name" value="zf-DPOE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23250">
        <property type="entry name" value="zf_DPOE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01159">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 19" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 19" id="KW-0235">DNA replication</keyword>
    <keyword evidence="14 19" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 19" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="12 19" id="KW-0408">Iron</keyword>
    <keyword evidence="13 19" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 19 22" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15 19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19 22" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 19" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase epsilon catalytic subunit A C-terminal" evidence="21">
        <location>
            <begin position="1524"/>
            <end position="1921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00057054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00065544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU365029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ4365814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="2245" mass="256407" checksum="A789ED3069A03D5B" modified="2023-11-08" version="1">MAPRRAGQPFRRVGKQGNTSYGPRKPKTVEASSLRSTEATSLNEKLESTRLANRIDESMGFPRFDSGKKRVGWLVNMHSTTIEDEDVPGGKAGVDYYFIGEDGDTFKATLEYDPYFLLAVKRGREQEVEEYCRRAYEGLIKNIKRVEKEDLSMPNHLTGYRRTFLQLSFANVNDLLAVRKAVSPVVEKNKKNINAMDTYAEVASGSGGIDFFDNDDYEYEKRATTTIDASDFILDIREYDVPYHVRVAIDKDVRIGNWYTVEAKHGVISITCIEDRLTPADPVVMAYDIETTKLPLKFPDAVIDQIMMVSYMIDGQGFLITNREIVSEDISDFEYTPKPEYQGPFMIFNEPDEKALIERFFEHIKEARPTVMVTYNGDFFDWPFVEARASVLGIDMYQEIGFRKNSEDIYHSDYCAHMDAFSWVSRDSYLPQGSRGLKAVTMAKLGYDPDELDPELMTPYASERPQTLAEYSVSDAVATYYLYMKYVHPFIFALCKILPLGPDDALRKGTGTLCEMLLMVQAYQKGIVLPNKHQAPKEAFWEGHLLESETYVGGHVEALEAGVFRSDIAYNFAVDPTAIDELMQDLDAALKFSIEVEEKKKMEDITNYDEIKAQIVEKLMQLKATPNRSERPLIYHLDVASMYPNIMTTNRLQPDSMIKEANCAACDFNRPGKTCDRRMPWAWRGEYLPAKRDEYNMVRNALENEKFPGKYPNSPSRTFQELSEDEQATLVRKRLTDYSKKIYHKVHDATTIEREAIICQRENPFYVDTVSGFRDRRYDFKGKQKVWKGKTEALKAAGAPATEIENAKKMIVLFDSMQLAHKVILNSFYGYVMRKGSRWYSLEMAGVTCLTGARIIQLARSLVERIGRPLELDTDGIWAILPATFPENFAFKLSNGKKLTISYPCTMLNHLVHAQYTNHQYETLVDPQTFRYEKHSDNSIFFEVDGPYKAMILPTSKEEDKNLKKRYAVFNHDGSLAELKGFEVKRRGELKLIKNFQAQIFKFFLEGTTLEETYGAVAKVANRWLDILDTRGATLADEELIDLIVENKSMTKTLEEYGAQKSTAITTAKRLAEFLGEQMVKDKGLNCRYIISKTPRNAPVTERAIPMSIFSAEESVKRYFLRKWLREDPGEMDIRTVLDWDYYRERLASVIQKLITIPAAYQKVRNPVPRVPHPEWLDRRIRQKEDKFQQKKMTDMFSKKALTNGDANIANNKISGDLEDFGAAKLTQAQSQMKKGIVTKMVAKRKEPEPAVPVQTDPYAALPKVMPSITEDYSSWLMYQKQKWKIQRQARKRRRQLFGEKPVDASDAIGSFFRNQAELLFISTWQLVQLRPTETPGEVKAFVLIDKKIHTIKIIVPRQLYLNLRSEDLPDVSISGCSVEKVVNHTLPNGHPSIHLFKLQMSEQTYVHEANSISALFNHSSVEGVYEKQVPLNIRAILELGSTCTFDESQKGVLGKGLEQGFDLSALRKVPTKQPYLTEAPLNYLYLYHVSSGDRHIYAIFSTWKSDAHIIIQNKSKDSQGMPNVDRIYSDALRRRMEDNQGEPWQTMIEYQDDIHFRTTAVTTRRKALLEIGDVIKKMKSEESGPVIVVMQSPNQALLSHDISILKDLPILPLHPDESDKQLPPLGWQSFIAKRLVGHYLDLGSWVAHLMELARYGDIPLCNLESNDPRFLIDVAYARRLQKDRVILWWSGQAKPDHAGYEKDDILAPLETVEMPSINNAGTYSSVCIDINLRNLAINTILSSSLVNELEGTDSVSFNPAAPSYDAANDGTNVIYSDNAFASAGITVLREMVKAWWVEAVQAGHGFSMADVMVQHLVRWVSSPGSFLYDRSLHYYVQMMSKKTLQQLMTDFKRVGSHIVFASPNRLLLQTTKAEVGNAYAYSEYIIKTIKSKPLFQFMELEIKEYWDYLVWYDEFNYGGKGCKEVVEEENQTIENIMCWQLAQFLPPTMQPTFNDWVVEFIELMHARKRPAPAADGSIPRLTQIPLRPLTVDPNEQTQILPKTFTKPLFKQISTLLRRQHTEILHPELAGDYVFPQLPGSHLKLQNPVLQLVKSIMQVLSLDRAISLEARMLRKELLNLFDIREFSQEATFANPSAALVVQGVICDECTCSRDLDLCRDSSLLPPVLPNNNTTTDTTTTATAPPPPKWKCDNCDANYDKLRIEEQLVSEVQKMVLEWCTQDLKCGKCKRLRSNEFMEHCACAGEWVGTKNRGEMKKKLNVYGNVAGFYGLRMLEAVVQECLEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y209</accession>
    <name>A0A9W8Y209_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DnaJ-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_008290</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y209"/>
    <dbReference type="OrthoDB" id="5894at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051964">
        <property type="entry name" value="Chaperone_stress_response"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054076">
        <property type="entry name" value="ZUO1-like_ZHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44029">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44029:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21884">
        <property type="entry name" value="ZUO1-like_ZHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="22"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="314"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="506"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="246"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="327"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="246"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="300"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="349"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="372"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="385"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="413"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="470"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4365670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="548" mass="61258" checksum="E6E61775C42401C6" modified="2023-11-08" version="1">MGASQSTGGGEAPGARSEVKTSYYELLGVERTATQDELKKAYRRKALELHPDRNHGDTERTTALFAEIQSAYEVLSDDQERAWYDAHEGDILRGGSGEGATEDHYQGNMRVTTSDELARMMGKFRGKVDFSDSPNGFFGFVRETFEQLAKEEEYAADFADIDRPSYPTFGHKDDQYDDVVRNFYAAWNGFATAKSFAWLDVYRLSDAPDRRTRRMAEKENQKYRDDGRREFNDAVRTLVAFVRKRDPRYTPNKQSDEAKAKAQRDARKAQAARARAEKMAQLDQEARALPSWTTARPADELEDESEEEIEEEHYECVACNKTFKSERQYEAHEKSKKHQKAIQSLTRKMQKDNAHLNLDEDAISSGAVSAGADEDDGVDDEGDDDKYASRSDIEARLAGLEKSTTSTSHDAGAEEGDSAEHGEDDDDDDDDDDDEYASRSDIEARLAGLEKSTTSTPHTEESNADSNDVAESKIDAPQEPKLGKAAQKRAKKAAKQAEENQSTPKHKCLGCDAAFPSKSQLFDHLQDHPKHVALKTAGDAGKKKKGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2E3</accession>
    <name>A0A9W8Y2E3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">18S rRNA aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="6">2.5.1.157</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 10" type="primary">TSR3</name>
        <name evidence="10" type="ORF">N0V83_008471</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Aminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine at position 1191 (Psi1191) in 18S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(1)-methylpseudouridine(1191) in yeast 18S rRNA + S-adenosyl-L-methionine = N(1)-methyl-N(3)-[(3S)-3-amino-3-carboxypropyl]pseudouridine(1191) in yeast 18S rRNA + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63300"/>
            <dbReference type="Rhea" id="RHEA-COMP:13852"/>
            <dbReference type="Rhea" id="RHEA-COMP:16309"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="ChEBI" id="CHEBI:146234"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>an N(1)-methylpseudouridine in rRNA + S-adenosyl-L-methionine = N(1)-methyl-N(3)-[(3S)-3-amino-3-carboxypropyl]pseudouridine in rRNA + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63296"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="Rhea" id="RHEA-COMP:16310"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="ChEBI" id="CHEBI:146234"/>
            <dbReference type="EC" id="2.5.1.157"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TDD superfamily. TSR3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.157" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2E3"/>
    <dbReference type="OrthoDB" id="10262062at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106388">
        <property type="term" value="F:rRNA small subunit aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01116">
        <property type="entry name" value="TSR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022968">
        <property type="entry name" value="Tsr3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007177">
        <property type="entry name" value="Tsr3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426:SF0">
        <property type="entry name" value="18S RRNA AMINOCARBOXYPROPYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN TSR3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04034">
        <property type="entry name" value="Ribo_biogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNase L inhibitor RLI-like possible metal-binding" evidence="9">
        <location>
            <begin position="50"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="16S/18S rRNA aminocarboxypropyltransferase Tsr3 C-terminal" evidence="8">
        <location>
            <begin position="87"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="221"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="246"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="373"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4365850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="411" mass="46595" checksum="E80CE0FA4F26D5B9" modified="2023-11-08" version="1">MVRHKKDFKSNNKYSNKPAKARGPPRPRRESDADQEQDAANPRAAKPAYKAACWDLGHCDAKRCSGKRLMRLGMMRELHVGQKFAGIVVSPKAKRIVSREDKDLMEQYGAAVVEASWKRIDEVPFGRIGGKCERLLPYLVAANPTNYGKPWRLNCVEALASCYYICGRPEWAESILETFSYGRAFLDINAALLKRYAACENEEQIKKAEEVWLAKIENEWKEQREEGAEEEDEWAGGNMNRRIIDESDEDDDEEEDGEDKNDDEDEEEEEEKIDPRNPYNIPESSDDEEEMAELRRRVLASKPFSNPAPKHANDDDDDDEKKAPQRIPRTEPAPAPAPANKSKDEEEEEEDDSDPPEENDAGDDEFDSFMAAQPTTDRTGITSTQRSKAMDKTYKATFSSGAVKAPSKQGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2T5</accession>
    <name>A0A9W8Y2T5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_008710</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2T5"/>
    <dbReference type="OrthoDB" id="10251155at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052073">
        <property type="entry name" value="Amide_Lactam_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47171">
        <property type="entry name" value="FARA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47171:SF2">
        <property type="entry name" value="TRANSCRIPTION FACTOR, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="17"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="56"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="120"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4365093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="701" mass="79292" checksum="FFDA4A8432D91421" modified="2023-11-08" version="1">MPPPKDKEQRARRTVKACVICHKRKIRCDIDEVQGDLCTPCQRDGYECVPRERKRKRFTFSPSPPATQRRLRAQHTDRSSTEDGQSTFLAVDPIENDTINSNKPFGTARLALSRDQNFKSEGGLGARRMSSTASLHEPSPLLERHPDPGYPTPHQSIPSNMTDTTYQSSNSVSYLGRLEYLRNDVPVNDDAGLPGKVPHRLSESDVEILRVQRVSELPPRAIRESLMDAFWTRCYPWSPVVERSWIEDRNPNQVSLLLQHAIFLAGSRVSAPLHNYSSEDFYKKARVLFWMGAEEDPIINIAAACLLHWYNPEGPERVSLDTSSFWLRVCIGLAYQVGLHREPTGKADAGLRRRIWWSLAVRDCLINAGHGRPRAIDLKLADVSPISVLDFDGAAASADLFSSYVGISCILGDLTQSYLRKHGLQEHKRSLEDRLFRWLKTLPEHLKLCKATEDRSLKPYSFEARQIHAQYFTVLVILNRPTDPKLSPSTASLLASSYIAGIFEDFMARDELRYLGPIFTFYCLTAGMAQLSCYRYSEMVDLAEENLAIMARALEELSSRWPSAVGSLKHLMDVREKVTQKPHLGLFPDLNFPESTAQFFTDFGPDLCRMWHPVHQRLPQMTPMASRELEMAGILQGLRTPNNQPLDIDLVANVAAQQQMMNSQVTTGATLEPTLLQPQEWFGPCGAGNWLLVDWDQGMGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2Y2</accession>
    <name>A0A9W8Y2Y2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Allantoate permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_008061</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2Y2"/>
    <dbReference type="OrthoDB" id="4454541at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF73">
        <property type="entry name" value="TRANSPORTER, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4366425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="450" mass="49564" checksum="8AF82BB5F70B8E10" modified="2023-11-08" version="1">MASPSDTKDAITHVEQAPQPTTVNTTTIPHNVDTAAKFLASAHVQDASFTYSEERSVLRRIDARVLPLLLGAYFFQQLDKSSLSYVSIFGLQKDAHLVGRQYSWLGSILYLAQLVMQPLAAFILVKLPTGKVIATAILLWGATLAVMASCRGPMKAKYLTEREKVIAVERLRANQMGIQSGHWRWDQVWETFLDFKTWCWFVAIVAISIASGGISTFGNLIIKDFGYTNFQSILFNIPFGFIQIVAILGSSLFATRFKRKGLTIALVAILPILGTILMLTVPRNSHNKGVLLFGYYLVSCLAAITPIIYAWHVQNTAGDTKKKCTSAMVFIGMCTGNVIGPLLYSLDDAPLYRPGLISNLVMFVLVALVALAIPVYLKVLNGRHERRREELGKSRVRVDESMLKKGEIGVAKEVEMEGGGGGGGGEQRAVLDNGFADLTDIQNEDFIYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y392</accession>
    <name>A0A9W8Y392_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008277</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y392"/>
    <dbReference type="OrthoDB" id="3689346at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="740"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="778" mass="88601" checksum="D3A0B19E51C20285" modified="2023-11-08" version="1">MQQSYLVRAHHPIYKDGVSRSLVELFRRHLRQNLHWNIGVRRIRRVLHTNRKFSSFGLIPVTAKDIELGKMRIDDEAKLHELPERKEKEDDRQARSEADKAAKQERAAKSKRTFKKTASNFHTDYVDSQFNVIEQKEDDVVRIEIHEDLRVKTYMLHLADVYLTYRNTREPGDVPVVKAKQDDSDSDSAMYGELEPDECHESDRVMDVSSLVWVDVLKSFSHVLIKIPWYFEALQASLAGGHRLYLLYLRHNELPYAVNLFTHRNNMPNKYEDLGKVMKANPQSFILIRGSDIPEQVKDVMNPSKILAHFNPISEFFNDPKQPYLNDKSVKPNEMCPTFSVAAVQVFEDYQYRPLLKTVFQETKEDAILTLNQIDDMTLSQMQQWSREKCTLQVKVATDTDIKEMKRLSAALFNMRIPQKLVPEYQAKEAALRPYATCRGAAVYLHYEYPHFRVAVTRARESVEWYTDTIQATAPTARIAKTHKMIAMFNYQGVVILNTGVDRMHCERFKVVLGALGPITSSPKAAVKDLTQSALGTSGTRQPLSGTIIEEDSDDEVKACNEKYAHIQALGNNLSDYGDAVTSADGGADDDWNTSSTQVMDNLESNSWNMPHGGVKINAWDTSFGISAWRVDGSVNNAVDEPHFQPPPLPVTAQTRDDLNLTEDPHDNCAEHDRLISSLFAAFQYRYLTVLYLKYRLPLHRFPYRGMLEFTMIHEILMIQSMHTTSGTLKISIVVCSEKPDPSDEGSSGSFYNLPGSNLDDGESAYGSDDGANRGYDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3T5</accession>
    <name>A0A9W8Y3T5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative phospholipase</fullName>
            <ecNumber evidence="4">3.1.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_008018</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = a 1-O-alkyl-sn-glycero-3-phosphocholine + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17777"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30909"/>
            <dbReference type="ChEBI" id="CHEBI:36707"/>
            <dbReference type="EC" id="3.1.1.47"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the serine esterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.47" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3T5"/>
    <dbReference type="OrthoDB" id="2363873at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003847">
        <property type="term" value="F:1-alkyl-2-acetylglycerophosphocholine esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016715">
        <property type="entry name" value="PAF_acetylhydro_eukaryote"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10272:SF7">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10272">
        <property type="entry name" value="PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03403">
        <property type="entry name" value="PAF-AH_p_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018169">
        <property type="entry name" value="PAF_acetylhydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR018169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR018169-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4366382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="428" mass="46897" checksum="011D89D5F6A7EA6C" modified="2023-11-08" version="1">MPVHENADLLKPTNTSNRWPVMMFSHGLGGSRNAYSHICGSLASHGVVVVAPDHRDGSSPLSIHHTPGQKEKLKRVEYRPIAHKPSTEVYEARDEQLRIRLWELGMIHHALLKIDEGRRVKNVAPEHHHHKKGEKDLLAMFTNLLSVHEPGSISFAGHSFGACTMIQFVKSVYYRTSSPAAGYKPLYTPLGDSSIVHQITPSSPVTLLDLWTLPSQSPDTAWLRSKPMPCYDAPTGGKNLLAILSEGFFNWSANFKETKRIVAKPVRSQSKFPDQPGPHIFYPISSAHLSQSDFGVLFPWVTTKVFGAKEPERVLKLNTRAILQTLRESGIRVANTSAQDLELDEADATNKSLAQDVSILSRSKDSVRGWVNLSADLEGGLDLTGVDRVEGKSLVGTPMEKVGSGQVSGGPGEAMVEGEALGQVVEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3U1</accession>
    <name>A0A9W8Y3U1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sugar phosphate phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">HRT2</name>
        <name evidence="9" type="ORF">N0V83_007535</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Metal-dependent phosphatase that shows phosphatase activity against several substrates, including fructose-1-phosphate and fructose-6-phosphate. Its preference for fructose-1-phosphate, a strong glycating agent that causes DNA damage rather than a canonical yeast metabolite, suggests a damage-control function in hexose phosphate metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>beta-D-fructose 1-phosphate + H2O = D-fructose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:35603"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:138881"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>beta-D-fructose 6-phosphate = dihydroxyacetone + D-glyceraldehyde 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:28002"/>
            <dbReference type="ChEBI" id="CHEBI:16016"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="7">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="domain">
        <text evidence="7">Subfamily III proteins have a conserved RTxK motif about 40-50 residues from the C-terminus; the threonine may be replaced by serine or cysteine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the damage-control phosphatase family. Sugar phosphate phosphatase III subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.-" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3U1"/>
    <dbReference type="OrthoDB" id="541375at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.60:FF:000002">
        <property type="entry name" value="Protein-glutamate O-methyltransferase C1393.13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10880">
        <property type="entry name" value="Uncharacterised protein PF01937, DUF89, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036075">
        <property type="entry name" value="ARMT-1-like_metal-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039763">
        <property type="entry name" value="ARMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002791">
        <property type="entry name" value="ARMT1-like_metal-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12260">
        <property type="entry name" value="DAMAGE-CONTROL PHOSPHATASE ARMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12260:SF6">
        <property type="entry name" value="DAMAGE-CONTROL PHOSPHATASE ARMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01937">
        <property type="entry name" value="ARMT1-like_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111321">
        <property type="entry name" value="AF1104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Damage-control phosphatase ARMT1-like metal-binding" evidence="8">
        <location>
            <begin position="24"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4367005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="483" mass="53619" checksum="81EC05450382B75B" modified="2023-11-08" version="1">MEYDTFSASQYKTSDATSFAHTSARERWPIIITQGIDDVHRSLHGTQDEQTLSEGKHIVSELAKLKYEVQHDRVLTPIPDDGESDVAAYNSELAARGSPKWHSVPWLYSECYLYRRISSIFKQTTNWKSYDLFARQKMATFRSSRPAVLELAARYKDIVTELEQKHTIQGAKTQADLEEAEQVLFVEMCEICLWGNATDLSLLTNLSYEDIQKLQGSEARKASEKNILVNDLSKAFKILTAAKKEGKKERRVDIVLDNAGFELFVDLILAGYLLASGLATTIVFHPKSIPWFVSDVLPADFGALLSALANSQNFYGSLSDDDQAAGKAPVPLTDAENANLQFLFQHWASMHGEGQFVLRPNIFWTAGGSYWRLPQSEPELYEDLKESELVIFKGDLNYRKLTADAAWQATTPFTDAIGPMGPGSGIRVLALRTCKADVVVGLPEGEDERIKAMAGGGGDSGARKWAWSGKWAVVQLSDGKTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4S1</accession>
    <name>A0A9W8Y4S1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_007106</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4S1"/>
    <dbReference type="OrthoDB" id="4142200at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000026">
        <property type="entry name" value="MFS quinate transporter QutD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF47">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="54"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="298"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="343"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="1"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="472"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4367523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="500" mass="54966" checksum="F5D81FF0799234DB" modified="2023-11-08" version="1">MNLRPSDHEGDRSDYFDNPSGVSQGAIGSALAAGSVVGSVMAGSVSDKFGRRDALLFACVWWLLGTSLQVATTSRGMLIAGRVLNGVTVGITSSQVPVYLAEISKHSQRGAIIIIQQLAIEWGILIMYFIGYGCSFIPGETASFRTAWGIQFVPCVFFMIGLPFLPESPRWLAKVDRTEEAIYILAHIQADGHLEDPYVVAEYEEIITVLTAERLAPKGWRKFILNGMWRRTLAGFSVQAWQQLSGANVMTYYVVYIFAMAGLSGNINLISSGVQYALFIIFSSIMFFFVDKIGRRTLLVYGAIAMGFCHFVVGGILGAHYTYVPEGVNGDENVQMLVSGAPANTVIAFSYLLIIIYALTLAPICWVYAAEVWSLETRAWGMGIASLGNWLFNFAIGLFIPPAFQNIRWGLFVVFGILCLLGATQFWLTYPETCGKSLEEIEVMFSHDGPRAWRTKKGHSRLEQETNAVATAQAKGNARESIENVIEQSKRGHGSKSETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y564</accession>
    <name>A0A9W8Y564_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Rac GTPase-activating protein BCR/ABR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">STB5</name>
        <name evidence="10" type="ORF">N0V83_007441</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y564"/>
    <dbReference type="OrthoDB" id="2399539at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052202">
        <property type="entry name" value="Yeast_MetPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782:SF7">
        <property type="entry name" value="PROTEIN STB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="9">
        <location>
            <begin position="173"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="72"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="141"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4366912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="280" mass="30749" checksum="2D572580C4C42383" modified="2023-11-08" version="1">MDRFRIAQSTPPTRQHAGLTCSLGISDTTSNEGLRKRLAWLQDFVNQQNLFGDWKPIEQVVTGTDLSRATNQLHSEGQKRNAEPLATTSPVTFSTLTPPATAPSPSLPATAYSNNEDDAASSDLRPAKRIRLLEPTITEQPPNATSSSTNATAPPTEFTPIHIKEPDARALVDAYFLDVNRAYPFVNRSMVLDALQANGNRIPPLRDCEATSTILYLIMAIGFTSLQRAGQVPPTTQWHFPIEYKEVLSECIIQETINTVQILLLLAIYSAGHSNRPDAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5K2</accession>
    <name>A0A9W8Y5K2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007841</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5K2"/>
    <dbReference type="OrthoDB" id="1263307at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052897">
        <property type="entry name" value="Sec-Metab_Biosynth_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37017:SF10">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37017">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="8"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="292" mass="31824" checksum="7534BAAA6979A7B5" modified="2023-11-08" version="1">MANSKPIVLIIGGGWHTKKSYSKLAAALEASGFEVHVPGHPSMNGSQPPNADLDTDSAHIRSYAEDLLKDGRKVIALMHSYGGQVGTNALHGLALKDRAKLELNGGISNLIYMTACTLPEGKAMIDMVKYHGHEELMPLAFDFADDMTCVSRDPKTLLIGADSGVSDQELEEYLSTLVRWNGQCMYDAMTTDRAAWRDIPTTYVYTTKDMTIPLAYQKWLVEEIRKEGVEVQTATVETGHCPNLSAAGEIAGIVERVAKGGILDTQREREGETRSKDDVKDAILGVDAAAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5Q4</accession>
    <name>A0A9W8Y5Q4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_006604</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM U1 A/B'' family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5Q4"/>
    <dbReference type="OrthoDB" id="266020at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030532">
        <property type="term" value="C:small nuclear ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12247">
        <property type="entry name" value="RRM2_U1A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000039">
        <property type="entry name" value="U1 small nuclear ribonucleoprotein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000029">
        <property type="entry name" value="U2 small nuclear ribonucleoprotein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10501">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A/U2 SMALL NUCLEAR RIBONUCLEOPROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="22"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="165"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4368248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="239" mass="26642" checksum="8DEBD92C61A0C552" modified="2023-11-08" version="1">MADKMEGVTTGGGPVKYPVPITTIYVSNLEERTKIETLKEALNAVFKHYGTIVDIVAKSSLKRKGQAFVVFDNEDSADRALQMNNFEMYGKQMKVAKARTHSDETVKRQAAGMFDEHKRKRLTLKDIKRAKEAAKAEANPAVAVEKPRAKTGAALVPDEYVRPNKTLFLQNIPQDVDEDALTAIFERFDGFKEVRLVSVRSVAFAEFENEQFAIAAKEATANTPIGAQGKPMKVTYQRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y662</accession>
    <name>A0A9W8Y662_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ketoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_005881</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y662"/>
    <dbReference type="OrthoDB" id="294295at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000398">
        <property type="entry name" value="Probable 2-deoxy-D-gluconate 3-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF5">
        <property type="entry name" value="2-DEHYDRO-3-DEOXY-D-GLUCONATE 5-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="5">
        <location>
            <begin position="13"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4368799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="252" mass="26847" checksum="BAC69F382A93AB1F" modified="2023-11-08" version="1">MASVQQLFSLEGQTALVTGGTRGIGQAMAIALAEAGADILLVQRDTSNQNTKRAIEALGRSATIYTCDLSSPPSVSALIPTILAAGHRIHILLNSGGIQRRHPSHQFPDSDWEAVLQVNLTAVFTLCRDVGAHMLSSPADLPLSRRGSIINMASLLTFQGGITVPAYAASKGGVGQLTKALSNEWVGKGISVNAIAPGYIATDMNEALINDEKRAEGILARIPAGRWGPGRISRARLCFWRVRRVRMLVGRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6H5</accession>
    <name>A0A9W8Y6H5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DNA topoisomerase 2-associated protein pat1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PAT1</name>
        <name evidence="9" type="ORF">N0V83_006618</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PAT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6H5"/>
    <dbReference type="OrthoDB" id="74835at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039900">
        <property type="entry name" value="Pat1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019167">
        <property type="entry name" value="PAT1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21551:SF0">
        <property type="entry name" value="PROTEIN ASSOCIATED WITH TOPO II RELATED-1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21551">
        <property type="entry name" value="TOPOISOMERASE II-ASSOCIATED PROTEIN PAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09770">
        <property type="entry name" value="PAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="mRNA decay factor PAT1" evidence="8">
        <location>
            <begin position="1"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="49"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="99"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="516"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="225"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="287"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="309"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="320"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4368262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="845" mass="94019" checksum="C3F4B8D31B500299" modified="2023-11-08" version="1">MSFFGFDTSLPRDTGHSRNAPGFGQHDAFAGLGGGAADDDVIDFEETYDGLGDQLDDGDDDLNDDTFGGGPATQQSIGRDFDFAGQTSMISNTLQEEQMLYQARQQPFQQRPPVPQASKPNRSGYESYKDPEYIPQLEARADIWGLKPKQPAAQRQSEPQPAPQPAPSRKMMSLDEVEAMMRAQSIGTPPAQQAPSQGPPAQYRQQQQFPHGQPPYPGMQGGPPGQFAPQILQRPQQQQQYQQPAQRQQPQQQQQQVRAELPGQSMQNQPPQHPTILQRQRPQSNEPSGQQQRQAPPPAQTQGPPSQPRQILQNPNRLSGQGQPMAQAAPQGNRGQAPGHSRGPSFPGMVITHPEQLLQLSEAERAAFLEEDAKRAKRNHKIALLAKDNGLMTPQDKNFITRIQLQQLMTATGNLDEKGPEAVIAEDFYYQVFSQIRGAPRQNPQQPASQFAQTYLFQTNNRFGARRHGRGGDNHMQRMEQQIQRAVEAAKARPKARQLVVEGSLGKIAFSNSKTPRPLLNLKRPETNDKLPKHHKSSIADRKEALRNIEAVYFTLMQMEDHERTLPPPIHESSPPEAIQAHMEWRSKIEALHKQLWLNTKIMEPINPHATTPHPFITILSHAKGKKAIPRLFRHIDEQERITIVTMIAVHLDGLSVVGHAIATPEEPLSAAIREEVELFSQTVLPPLSAHISEAPLNIVTGLLGLILDRTNLHVVARSKIGLSLLTILISRAELLKQSAPELATDWEQWTELYNRLFDTIEPVLPVIFPGTINDTDDIYIWQYLAAMGVGASPEQQQRLVLGVKDRVMETVSVSKALPAEMASARLANVNLFMRAIGLDVELLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6S3</accession>
    <name>A0A9W8Y6S3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_006198</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6S3"/>
    <dbReference type="OrthoDB" id="7700931at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000042">
        <property type="entry name" value="Fasciclin domain family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050904">
        <property type="entry name" value="Adhesion/Biosynth-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900:SF125">
        <property type="entry name" value="FAS1 DOMAIN-CONTAINING PROTEIN YLR001C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900">
        <property type="entry name" value="PERIOSTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040987947" description="FAS1 domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="3">
        <location>
            <begin position="142"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="3">
        <location>
            <begin position="272"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4369114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="481" mass="53615" checksum="37A42313A0593349" modified="2023-11-08" version="1">MRFTRIVPLLPLASALVITEAEVFQNLELSGKRVVDNAQSVLDNAQNVLDDAFGRVKESANDAYSKIHQAGCDVESWLEGSTFDDSEVDFEEGPHHPPPPPHHGKPPHDKPPHGKPPHDGPPHRGPPHHGKPRHPPHHGKPNRTVYELINESKYTTKLAKWINEYDDLVDALNGTKANYTIFAPTDKAFEKIPHHGKKPSKEVLKDILLYHVSDEFYPAGRVLHSYTIPTLYEPEKLGHKQRLTVRVTLKGPAINFYSKIVAANIFGTNGVIHGVDSILIPPPNVASIIDLLPGEFSTLELALVKTGLFPKLNSTEHPHEGGTFFAPSNFAFKKLGPRVNAFLFSKYGEKYLKALLEYHVVVNQTLYSDVFFDATGKNKDKDEAADRPPYYHYDLPTVLHGKYLVVDVTRYGPFVSIKINGFSSVTVHDGVASDGVIQVVSNVLIPPKNANGEQVFWEGEEMSVDDIKERLQPFVNENIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y717</accession>
    <name>A0A9W8Y717_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lytic polysaccharide monooxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007136</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y717"/>
    <dbReference type="OrthoDB" id="2342176at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36182:SF2">
        <property type="entry name" value="LYTIC POLYSACCHARIDE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36182">
        <property type="entry name" value="PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G10930)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040926008" description="Lytic polysaccharide monooxygenase" evidence="2">
        <location>
            <begin position="23"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="237"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="289"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="315"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="38726" checksum="E3DA4A2840D575CF" modified="2023-11-08" version="1">MMSQKSTLLAAMLVMAPLAANAHMIMANPLPYGHPNNSPLDPSGSDFPCKSVPYTVNTMNEWPVGSSQYLSFTGSAVHGGGSCQISVTTDKALTKDSKWKVIYSIEGGCPASVAGNLDETKNSDGRFNFTIPSELPNGEMSMAWTWYNKVGNREMYMNCGPIKVSGGANDNTAFDALPDMAVANLAITPNCKTKESSDYTFENPGKYKISAGTGPYVSLCGGAPSTGGDAPAPAPQQPAASQSPPANPGNSSACRAPSQAPVQTSTLRTMVTVTAPLGPAPTSAQGNVSAPSANSPSAPQPSQAPAAPAAPAPAPSSGTANGSSCSPDGSILCNGETQFGICNHGTVVWQAVAAGTKCQNDAIAKRDYAHRNMRTAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7A2</accession>
    <name>A0A9W8Y7A2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005441</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7A2"/>
    <dbReference type="OrthoDB" id="3799761at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="493"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="496"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="534" mass="58931" checksum="1419939CB5A92E5C" modified="2023-11-08" version="1">MPPSKHDRKFSNASSWSQHTRNASRSDYTPVSPPRNPQAPSFPTITSTSTNAERRSGSFEYDAPGTTPYWDDTYGVIYETPEIPPRHPGRTYAQALRRGLSLHDVSEGHVESDQDTPQREFLQHTASQNSYPQSIGKQLSRKGNFTPWSDATDDRHAASSNGSIAGNQISSSRPALSSKDVNQKLSHELKEKTKAKASTRASLKNSIKLPPAWKELRPSPASVLKQEAIFGIPKTAAKEARPMSALVEEPDPLRIVKKKSTSRAVNRIPSNSSPMLERVRQLRDLEQKAILAKLEGDLMNVKATESTAEDDDIDVFPDAEEANFDVFTGGQTKQQSMAHKEARHSQHTEEFIPNIELSDSDWAKPLYALANPPAVPPKSPKRNLNLNAQRNSMVATIHSVDDSDESNAQSDDSLVDTMSGMRFVPNLPPTPPKHRPFQRKSAVPEGLDLKQIQAAKEKLKEKPVITVAQDAVPNVVGVGVVNSKRNVQLVKASRESVATTTSSISDDFEQLGSPGKDDTEHNGASRKWYKGFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7A9</accession>
    <name>A0A9W8Y7A9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_007272</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7A9"/>
    <dbReference type="OrthoDB" id="2019504at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010301">
        <property type="entry name" value="RRP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13026">
        <property type="entry name" value="NNP-1 PROTEIN NOVEL NUCLEAR PROTEIN 1 NOP52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13026:SF0">
        <property type="entry name" value="RIBOSOMAL RNA PROCESSING 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05997">
        <property type="entry name" value="Nop52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="210"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4367687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="233" mass="27498" checksum="2EAB33E2D7599B97" modified="2023-11-08" version="1">MVTDKKVRDEAFGSLRTYLGAQSEISELDLLKLWKGLFYCLWMQDKPALQQRLSRDLASLVSTLRDPVVMPFLRAFWLTIAREWSHIEALRLDKYLYLIRQYVNVSFQFLSRNNWKKEVVEEWNNVLEEVPLECKDMKIPNGLRYHVMDVFVDELEKVGGSEWEEKEERKQTLETLVQPVEKMAKEGKLKVLRVAAKECLADERLRAWRGQEDEAIDEPEEEDEEAEWGGIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7B3</accession>
    <name>A0A9W8Y7B3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cohesin loading factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_005456</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SCC4/mau-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7B3"/>
    <dbReference type="OrthoDB" id="5565328at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019440">
        <property type="entry name" value="MAU2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21394">
        <property type="entry name" value="MAU2 CHROMATID COHESION FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10345">
        <property type="entry name" value="Cohesin_load"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="127"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="874"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="162"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="878"/>
            <end position="897"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4369694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="897" mass="100321" checksum="9A5BF7598239063F" modified="2023-11-08" version="1">MDPRYNWPPQAYMNPNGQYVPYTPPQPTNGYPQNGYSQQYPQMQMQMPQGYPQSQPYPGHPQAVTSAPGGQQYSQMPPQMQHQQQPQFRAQPQVVIPPKAPNFASPMQMQAPKIRHVQMPVQRTGNVGIAQTDGTSDRHRQYRAQQAPAPPTSKPTQSSQSFQENGPRPQQRNISTPVSNPPQQRTPLQSQSTPQQRPSAQSQTPSSQNRGQPVQQPSSKPKSHPQVVISRPTSHQLTPTKHVHTPQKALPADLSVMLLSAADEYVAAARGMGSLIIRDRKEADLRQYYKLMSTAMGCMDAVLKDFSMLPRDEAKLRLRYATLLVEETDNTTDIDAVISKQVSLCGRCRLQDLKYATMHLQARYQFKTNHRAALKSLDKPISEAETFQHIPWVYAFRFLKVSLTLQIPGRVEAVSALQQLHAILAHAERKGDRAIYVACSALEALVHLRSGTPDRLEQAQRAIAAARSLQLQVSAKQLGSFGTLIDIIDIACGIQHGMPSSQKSTALIESILGENDKTLGQDNGVFTVLVERSPGGNLTFDTGGVFRKSADGRDELVFAWLPKEDLKTLCFHICALDQNVHEKGLSYMKEAHQRSRSALKRQTLYATPISVALAHMNWNRLLDWHSMFTIGLIACFREDRTTAGEALTILRKRISKPPHDNQDRFARTLLYLSAINDQASGYLDSAFAAYSSDTLALTDKGSTTDSKMDIGILAALNRLLIVRNPAHSKHYTGGTLLTQLRPLCENHPNQYIRMAFRLVHAISSEEAAINRQKTLMQTASNRANDIFRNTQNREFVIMALCYFTMRFFADQVGEKSIQAIRATRQNAKLSRKPLWMAVAFGLCMSTYQRNGLLEEAHKAQLEFEAVRPQLPPALRGEEDIDAEGEEDAEGDVNDIYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7D4</accession>
    <name>A0A9W8Y7D4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA amidotransferase complex subunit Gta3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7D4"/>
    <dbReference type="OrthoDB" id="5522061at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036113">
        <property type="entry name" value="Asp/Glu-ADT_sf_sub_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003837">
        <property type="entry name" value="GatC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049545">
        <property type="entry name" value="Gta3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004:SF0">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20978">
        <property type="entry name" value="Gta3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141000">
        <property type="entry name" value="Glu-tRNAGln amidotransferase C subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl-tRNA amidotransferase complex subunit Gta3" evidence="1">
        <location>
            <begin position="76"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="208" mass="23390" checksum="C826325039C3009F" modified="2023-11-08" version="1">MASRRAFFCLEPLLRPVRVSQTRPFTTTPLPAREISPRNTPLVHKNKGIHPDELEQLLAEPTWSVESLLPPKTRAPDAPEITSQQLHHLLRLSALPPPENPEQEQKMLDTLAAQLHFVGKIQQVDTTGVKPLRAIRDETAAAEQEQTITLGTLKEALGNEKVVGHHYKRIQRDTTPVDAQEVENWDVLGSAERKIGKYFVVESGRPQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7I8</accession>
    <name>A0A9W8Y7I8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Phosphate metabolism transcription protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">VTC2_1</name>
        <name evidence="9" type="ORF">N0V83_005563</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7I8"/>
    <dbReference type="OrthoDB" id="6493944at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033254">
        <property type="term" value="C:vacuolar transporter chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07892">
        <property type="entry name" value="PolyPPase_VTC2-3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14480">
        <property type="entry name" value="SPX_VTC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.100.30:FF:000002">
        <property type="entry name" value="Vacuolar transporter chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.30">
        <property type="entry name" value="VTC, catalytic tunnel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018966">
        <property type="entry name" value="VTC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042267">
        <property type="entry name" value="VTC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF2">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 3 COMPLEX SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09359">
        <property type="entry name" value="VTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="686"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="711"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="751"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="8">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="87"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="573"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="594"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4369799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="800" mass="91065" checksum="465C1844A5E66936" modified="2023-11-08" version="1">MKFGTTLRKSVYAPWKDKYIDYDKLKKLLKDNEDDESWTADDESAFVDELANVQLEKVHNFIKDISQKLRDRTSACEKKLEPLAVGIQSDDKQVGEEGEGLFAQGGNGPKKPEPSEQERQKMLNEVLGELDSITKETKELEGFSRLNFTAVIKATKKHDKLRGNSYKLRPFIDARIARHPLDTEDASPLLYRLSALYSFVRQSLEGKPKEALSFAEGSTSGEGFVSHKFWVHMDNLFEVKTVILRRLPVLVYNPQSSKIAEGTQRDPTITSIYFDNPNFSLYSDKVNSKPDAVSLRLRWYGQLADKPEIMFEKKVIKEGDAAEEVRFPIKAKYVQSFLEDNYHMEKSIDKMEYRPTKDQKQLDYFKNAVTDIQSFIKEKNLQPVLRANYTRTAFQIPGDDRVRISLDTNLALIREDALDLDRPCRDPDDWHRRDIDSAHMEFPFKAIKKGEIHKFPYALLEIKVKGMKKYEWIEDLTHSHLVKESPRFSKFVQGVAKLFEDNVNTFPFWLSEVDNDIRKEPEKAFDDEQEKKRKAAEDEFAVGSLFGARASPSYRPSIASPVGSPAAARSITKSASAAAHTQPLINPPLPTVGSHLSQQFQQDNGNRVHEEVDSDDDEARPIGKTKSSGLGSLFPSFSTSKYARKQQRKIKLPPGIRDPGVWIKDQGPVRVEAKVWLANQRTFIKWQHVSVLLASLSLGLYNAAGEGNNIARLLAVVYTCVAAFTLAWGYGMYWYRSKLIRERSGKDFDAITGPLVVCVGLAVALVLNFAFKYNALIKERQHHHDHVNTTFGNEQTELRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7Q4</accession>
    <name>A0A9W8Y7Q4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Aspartate aminotransferase</fullName>
            <ecNumber evidence="8">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">AAT1</name>
        <name evidence="10" type="ORF">N0V83_005863</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7Q4"/>
    <dbReference type="OrthoDB" id="6752799at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006533">
        <property type="term" value="P:L-aspartate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000026">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000181">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF22">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 10" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="9">
        <location>
            <begin position="53"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4368781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="425" mass="46461" checksum="577AB4D3868EBB23" modified="2023-11-08" version="1">MLASSRTALRQATSKQLTLKTSARAVSVWSQVPQGPPAILGITEAFKADSNPKKINLGVGAYRDDKGKPYVLPSVRQAEKKVVDGALDKEYAGITGVPNFTKAALKLAYGSDSTPLAEDRIAVTQSISGTGALRIGGAFLERHYPNAKAIYIPNPSWANHNAVFKDSGLKVEKYRYYNKDTIGLDFEGMVADIKGMPKGSIVLLHACAHNPTGVDPTEEQWRKIAEAVKEGEHYPFFDMAYQGFASGDTNKDAFALRHFIKEGLRPCLAQSFAKNMGLYGERVGAFSIVCESADEKKRVDSQIKILVRPLYSNPPVHGARVASEILNDASLNKQWLGEVKGMADRIITMRALLKENLEKLGSKHDWSHITSQIGMFAYTGLKPEQMETLAKEHSVYATKDGRISVAGITSENVGRLAEAIYKVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y835</accession>
    <name>A0A9W8Y835_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y835"/>
    <dbReference type="OrthoDB" id="3799439at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="491" mass="56315" checksum="327EEBD1F117713B" modified="2023-11-08" version="1">MPPAKKQRSGVSPFQTSIRWKPDTSRDASKPAKPRGDRRRLVIGTEFDGYGVECLRSEAEAIVDQHCDYEVLTRFTKRSDPHTEPTVTDIVSRMEDEFDEANVMIEKFIGDLKKKKSALSSSIDDMLKTIGKRNKWWEETDIFAHDVPEELVHKIFLHCPLRDYAAYANYITKFTDSMELMDSEEPEFGPLIDRLLKEISRVAEVHKRTAMLLLINLQTVVIWAGPYPDLYITKTQEVLVSKIHEAVSKLLEVAGKEKEPEFMDIANLCDIIRQNMEAEPCKTGTEEPEDEHEIVENACSYNIFLETLDLLQRHAYFEIRANVRFALGRRLPAELVQLVFEHTMEIVGIGYDPRIILDATVGTASPCQTCMLYCKHEIATKNVDFKPEYELEYVGPESRQYAFAGAYVCLAKFPHGVADYKELCEKEDSASSKIFNDDLWELCQEPGYDGMLPDDHPNRYDEGSPHYGLQDYEDYGDYFSDSEESEVYGPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8R2</accession>
    <name>A0A9W8Y8R2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8R2"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="167" mass="19484" checksum="4CA9ABEE24E76405" modified="2023-11-08" version="1">MLTKASQPQRPCTPVPKHKKLNSKLSSTLSDLVMADYDMVLASVNDLLTQVGLKHKRHPKDKQLSILLQNVTDLKNDTRQYPRHMSTLAKSEPLYQMFKEKYKDKQAFLECNAGSLDGYQESLKNFKDLESMRVYLRRKGWELAESIVEGLEKFEEEFEEVKRRLYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8R7</accession>
    <name>A0A9W8Y8R7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005378</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8R7"/>
    <dbReference type="OrthoDB" id="3800613at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="257" mass="28773" checksum="AEC9AEA7CA35C652" modified="2023-11-08" version="1">MPKYREPLWLELVVNHFLNSIAAHFDRAFRNTPLEYWLLHPEGQVGTLFMSIILVALVWMILLEYSSQRAKARTATSAPTHIHHTTSIVHKHTHIHNRAHRADSSASDLWSIEWNETPVMRYLASSSPSPNRVTRPFGTPTASNRTIASSEFETPRSLRPTHSQIFSSLAKAAPVSYDTLQETGMLSSDGAPTLKWGLTKRMINAGDRVEEKKTQGKGKGKGKGKGKEVVAYWENDVGMKGGRAKLRQELDGEDGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8V1</accession>
    <name>A0A9W8Y8V1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAR-17a/AIG1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_005041</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8V1"/>
    <dbReference type="OrthoDB" id="1898221at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006838">
        <property type="entry name" value="ADTRP_AIG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989">
        <property type="entry name" value="ANDROGEN-INDUCED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989:SF16">
        <property type="entry name" value="AT02829P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04750">
        <property type="entry name" value="Far-17a_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4370520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="25980" checksum="87F5AFC21F93CA9F" modified="2023-11-08" version="1">MGAAVKSSRRHPLQRLDSPSKGFSGALHNESFGWHLQFLTIIGITICTACFTFGVLADISGSHTCYTLKNYLALVAAPLEIVISLLYWGLRAIDDALVVPPDLPMPPLLYDLGYHLVPAVVLTLDAILLSPPWPTMPMNPQAPMIMLVTSTAVAFAYWWWVEICYSYNGYYPYPLFGVLTTLQRVGLFVLSGITMWVVGGALRALYAWVNGYESVEELEKFRRAQKMGVEGKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8X4</accession>
    <name>A0A9W8Y8X4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LCCL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_004975</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8X4"/>
    <dbReference type="OrthoDB" id="441660at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.20">
        <property type="entry name" value="LCCL-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051957">
        <property type="entry name" value="CRISP-LCCL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004043">
        <property type="entry name" value="LCCL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036609">
        <property type="entry name" value="LCCL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31331:SF1">
        <property type="entry name" value="CYSTEINE RICH SECRETORY PROTEIN LCCL DOMAIN CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31331">
        <property type="entry name" value="LCCL DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G08630)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03815">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00603">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69848">
        <property type="entry name" value="LCCL domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50820">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="316"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="361"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="469"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="511"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="LCCL" evidence="3">
        <location>
            <begin position="185"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4370457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="651" mass="71242" checksum="078395AB75C9A66E" modified="2023-11-08" version="1">MAAARDIPLKSGSTTVPIDLDLERGTRGHDDDRITSSASSSLEPEEAVSKAAAPNLRHSLEIDQGTRRRQSTLGLQWRRRVEDKMPAPVTRWGNRLVEWMRGPKPLRTYRITPLLERWQTLPGRYSARLPKWLRICIYSALCILWVVLFGVILSEYSLPEDIGGFGAPVRLGCVDELWPSPQSCGLDGRNCLPFSNSSFAFNCPADCLSAQVLNPRAIGNQSINYRSLVVGGTPDPSANGALVYRGDSFVCGAAIHAGVISNGNGGCGVLSLIGEKSNFGAIQRNGILSVGFASEFPMSYTFNQTSAKCQDPRWNLLILSTIFTVVFGLFTSSPATFFVPVFTILFFQIAMASDPPSYSNYPSLASTTIGRFLPAAFVAALFYRFSVRKTLRGCKAHVEKTVLWIGGAWVGALSNYTFEQIPIQRLTGHDIRQQPGAITALIIIVLVLFGVVLYQAWCLRNEGRLPRYLALYAILGISLGILAAIPKLSLRIHHYILALLLLPGTALQTRVSLLCQGLLVGLFVNGIARWGFDAILQTAAALQGDAQLGSGVPSVLEPIINGTNITFMWEGLLRGYEGVSVVVNDVERFRGINQVGNGSFTWSRQALDDPEYFRFGFINYLPFGSVSYSDFTKAGTWWPNGTWGGIPPGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9N9</accession>
    <name>A0A9W8Y9N9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP synthase subunit 5, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ATP5</name>
        <name evidence="9" type="ORF">N0V83_006259</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase delta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9N9"/>
    <dbReference type="OrthoDB" id="1262810at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.20">
        <property type="entry name" value="N-terminal domain of the delta subunit of the F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01416">
        <property type="entry name" value="ATP_synth_delta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026015">
        <property type="entry name" value="ATP_synth_OSCP/delta_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020781">
        <property type="entry name" value="ATPase_OSCP/d_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000711">
        <property type="entry name" value="ATPase_OSCP/dsu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01145">
        <property type="entry name" value="ATP_synt_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11910">
        <property type="entry name" value="ATP SYNTHASE DELTA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00213">
        <property type="entry name" value="OSCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00125">
        <property type="entry name" value="ATPASEDELTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47928">
        <property type="entry name" value="N-terminal domain of the delta subunit of the F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00389">
        <property type="entry name" value="ATPASE_DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4369175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="232" mass="24338" checksum="2B19E955AE124CDA" modified="2023-11-08" version="1">MSAARAFSSALRAGAPRAPIAARYAVTRSYAAAAAPSGAATKPPVALFGVDGTYASALYTAAAKTNALDPTAKSLESLSSVFKKDAKLTGILHAPALSVSDKQQIVQELQKYVGNADKEGIVKNFLATLAENNRLGVLEGVVEKFAVLMGAHRGEIELVVTSAAPLDNRTLSRLESAISKSQYVSSGQKLKVVPKVNPEIRGGLVVEIGDRTIDLSVSSKMAKMNKLLKDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9T8</accession>
    <name>A0A9W8Y9T8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mg2+ transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_005486</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9T8"/>
    <dbReference type="OrthoDB" id="1046782at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494:SF1">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4369723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="33322" checksum="3C848658005DA37E" modified="2023-11-08" version="1">MFCFGAPVTIRDRFQKLKSVATCDDLLQDPYALLEIVLEEMYKVMDQTGWVISDIFGAIETQSLGMASKPGKATKELPIDHFTGLHNLAKHTIYLRENCESALATLDDLRDHHRSVIGDRPSSSQEHTRQALKYRKTLFQSTQRRLASLDARMANIIQLSFHIVTQGDSRLMQSENQSMKTIAVMTLLFMPLSTVASIFGSQFMKLQDEAPFHMKVSQDFWLLWLIAVPLTVVVMVIWRVWYMDARGRLVDEIPPSAPGYMGWNTLKQTLRRESGKEKEQYTVTTREVTSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAI1</accession>
    <name>A0A9W8YAI1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Target of Sbf</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TOS1</name>
        <name evidence="2" type="ORF">N0V83_004001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAI1"/>
    <dbReference type="OrthoDB" id="118256at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018805">
        <property type="entry name" value="YJL171C/Tos1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31737">
        <property type="entry name" value="PROTEIN TOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31737:SF2">
        <property type="entry name" value="PROTEIN TOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10287">
        <property type="entry name" value="YJL171C_Tos1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cell wall protein YJL171C/Tos1 C-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="142" mass="15760" checksum="5CFF8D4BF733B11B" modified="2023-11-08" version="1">MDHYNNTGTDQGMISDAPALWFLNAAIPRILQYGNDRNNIPCSCWSTGCGEFDAFEVLSNGQEKAKSTLHRQNNLEGGDSNYFKRPVGSTLKFAVVWNYPNIIALVLDDSFDFGSTLSDDQVQSLVSYDPNSWVHSLFAIGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBN5</accession>
    <name>A0A9W8YBN5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003817</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBN5"/>
    <dbReference type="OrthoDB" id="3656752at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040922955" evidence="1">
        <location>
            <begin position="20"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="163" mass="17487" checksum="B5AEDE608676B72A" modified="2023-11-08" version="1">MHATTTLAAVLAFATSALAGPVSVKLDSRDPNNFRLYSVHQHQNTCAVDICQTIDNYYVNVADDCNGGGCNAKESTLPWDDSYCNADFPVCDRTLQLVSRGDGDCGKLSDLSASNNGQPYATITENGVDVGTCSVDFSRHYSRTCGALGGGFEFQSHVYCAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBQ9</accession>
    <name>A0A9W8YBQ9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_003363</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBQ9"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="3">
        <location>
            <begin position="55"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="310"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="318"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4373072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="487" mass="53647" checksum="B419FA8202BB9286" modified="2023-11-08" version="1">MAEPDRLCDYQAMFLIEVHSHYRARRAAKTLSSRFEKVYHKSTEDFRSVTSKIADVVYPPENATLDQWVQWIELATWQRLLLSCYVLETQQAILLAREPVPSLIQETGLDLPVPTHCSVWDATDPTEWAFAAHRFSHSPRYVYEVTADLTVPPFDSFQSSVLLAAHCNRIDSPAPYTSSSTRLDIEHLLDTSPITTRQLLTTKLVQVTPIRALLAVSGESWILSEKVPSAQDFTAMKTTLRGWLLQLWSTSATKASTVAIKEALKLSIDILQNALKEQQNSITLDWGTGMGIYFAALVLWAITTTANTRIKGPQKTTQQPLRPQSQSPLPFATGSSPSVAPTPIQLLNQFPRHPPSVTSSPTQPHNIGPLQSRETSPASPPSIDCNTLLSHAQITINTITFLSTALLDFGSSDSLAQLPLNLARCQTGCISLLLWVKLRLRGVSLEDQSGMADAWTARPGEGLGELLDGVTGSLERILGKGWSGWGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC60</accession>
    <name>A0A9W8YC60_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003621</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC60"/>
    <dbReference type="OrthoDB" id="3764174at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="274" mass="30833" checksum="95AD648DFCAED9A1" modified="2023-11-08" version="1">MQAHHQHPQTKDDDILSYTAQQRFDAEYSPCHPLSCFHDLLCGHRIQVEYNSETCGINCARQKINKPFVCPECLIDGVRVEVALHGLDLRAPGEDVDMGGYQSTREDAIQKIADMKLKQLLAMGRRMCKMTTKLADPKLQFFHQFLIEEGFEGIVEDGNTDLTTATRYKRPNAAKGKSTRGPAWKHREPAQQDLEGEAEVTVTLGDVMEEFQAGTRQTELLKEERGDDTARIDSAMDGLVEQFYQTRVALPVEDEATRAVREAFELCELTGGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCX8</accession>
    <name>A0A9W8YCX8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002823</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCX8"/>
    <dbReference type="OrthoDB" id="5429993at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="247" mass="27089" checksum="0F78DDD1A5143B69" modified="2023-11-08" version="1">MLPQLGQPIYSSRLEGLSNDPDRDAKSKEPDLVATEPENVPISKDVGVQGSVPGRLRPRSMYQYGPTRTERVTRTDDTPPSRSMLPPASASKPSEPKPAGLSRSRSLRRPGGAMQSAQPRETTVQARTQSVNTTGITRKETMKSDTGSERPKSLLVAPNSNLKTNIIPRDRTPHDTKVSTRIAHNRSTSIKARAEGLSGSARTATTARAEEPLVPQPRQRQPEQEEPKRIARPGLLNFTTTFHTTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD20</accession>
    <name>A0A9W8YD20_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_004090</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD20"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047499">
        <property type="term" value="F:calcium-independent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185:SF1">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="5">
        <location>
            <begin position="654"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="184"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="992"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1142"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="195"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1038"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1066"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1087"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1148"/>
            <end position="1163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4372316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1182" mass="134389" checksum="ED35378A538BC357" modified="2023-11-08" version="1">MDFCDQCWGKQGPHKPGRTGPDGLPHEKANPTIVKRLKDILTPPQDHGEQQTLHVEDEDTTWFGIARDNQNRPIFQDYDRYSSIMADSNSGEYKYRYPQLVSFIGQTGAGKSTLIKMLIDQQERKHGPRDCTFPSPVAGSLSNGSVPTSGDVHLYSDPSTYAAEYPMLYADCEGLEGGETTPMAAQYRNSAPSPYKDKGKDHHAPKEPRRRRLVSKGLHCTQRDIKWANSPDTLKRQYAVTELYPRLLYTFSDVIVFVLRNPKTFESTVLSLLITWASSSLEKSLNQPTLPHAVLALNATDTKVNQQEWDPDYATDLLMSNVASAITRDPTYRELKQYWEGRGKSIQSMKDLLECYYSSITVVRVPGEGRYMMIDEQVKKLHDVVSKRCTESFNAKRRSRMLSNSETLNVYLQCAFDHFAQDLHTPFNFMDVSFKINPIPLDFGGNILKLAVAMKARYDDPRRIFKELSFMVSSCILLDCVRQSLKALEDFCAIFWPCTFRNKRGDRCVNVKERHTKGHQNQRGHIIGTGPYEANFTFEDFAEDWLRYLQTSLSDFQKDLSSKMMTSPAADEIVITTKLHQANVNNFYHHVGGAQNFPLEEALSESFKDEAIFGGVPETPVGCTRKVAVTSATETGEEAVIFTNYNRADDEQIGYRLIRPDDPKNDLMIREAARATSAAPTFFKPFRNSRTMEGFLDGAVFHNNPVRIANYESKLLWPDAEECHPDILLSIGTGHHEADTDGILKPTRSDCRRLQIRKVINQVKPTTRERRSIPGFRGFTEVESWVAIFKKRVESVLDAELTWKEFRKDVVGTSSPIAAERYIRINPRTKNRTPKMDDKTQVHFLHDEVKSGLRTHGMRTKIERIAHRLVASSFYFEKTGPSREAGGHITIQGDYIRKHQQRSFQPFFRIQEVKHDQTAQDIYITAHNIREMIDRGLFHLESIVIPMTSESSLLSMDLHLMDERTKVTVCSGFPISGFPRNLADGEPLKRTASPAALATPERPKMSTKRQSLRDKRHTHHRISLERPASDSNISYTLHSNSSHSSRNNSLGPSGLGFPETPDDIPDWVRRRSEASRRSPKPVLQRFPDQEEHYVPQLSPRREELYELDASGGPPLPLQADDEAIVRAYEQGQRHDSLVAPRITRSENDLSLTPTQRPSLSRTDTGTDEEELARALSLSLQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDY5</accession>
    <name>A0A9W8YDY5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7053 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003379</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDY5"/>
    <dbReference type="OrthoDB" id="4276610at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055481">
        <property type="entry name" value="DUF7053"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38117:SF1">
        <property type="entry name" value="DUF3074 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38117">
        <property type="entry name" value="NACHT AND WD40 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23155">
        <property type="entry name" value="DUF7053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7053" evidence="2">
        <location>
            <begin position="3"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="268" mass="29212" checksum="9C8C8423C0BAE479" modified="2023-11-08" version="1">MSSKHHLHVAASIPAHLTPADIIAALHDHKTALTLQALTTGHTKADSTAPETLKDTYWYPPDQYPITTYNVRECITLLPGVGEFGKKYITFPSCFQDTKAGIKTRADASGVIVRAEFRVVRGGAGAEVEGEGMGVGDAEWVLVEDVEVSCSWWLMPFVKGKMEQAHRDVCRKVVEKVEMEKRQEAVASSAGSVSKGKGRADMMTGMDRGNFGDASRQPHQAESYYERYGKAQRDGGGGGTEGLVEADAVETQRVEMPAPPLPEKITYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE68</accession>
    <name>A0A9W8YE68_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA replication licensing factor MCM2</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">DNA replication licensing factor mcm2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Minichromosome maintenance protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">MCM2</name>
        <name evidence="21" type="ORF">N0V83_002173</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE68"/>
    <dbReference type="OrthoDB" id="844at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17753">
        <property type="entry name" value="MCM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000002">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000138">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008045">
        <property type="entry name" value="MCM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF44">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12619">
        <property type="entry name" value="MCM2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01658">
        <property type="entry name" value="MCMPROTEIN2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 21" id="KW-0347">Helicase</keyword>
    <keyword evidence="9 21" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="20">
        <location>
            <begin position="487"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="711"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="33"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="53"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="720"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00074927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00078186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ4375091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="867" mass="96743" checksum="321644370280FF58" modified="2023-11-08" version="1">MSSPLRSNMSSANRGVGPSSGRNKRARDDGDLPSSAAHPPSSPMASSPPMLPQDDDADMLDDDDIIRDVDDLDEDAEEAEGIDLFADDFENDYRARENDAYEGQGIDDEGDYEELSLAQRRELDARLNARDREARRRMPAAFLPDEDEQDGLADLMGRRRVRRHRYDEEDDMDMGDDIMNEELSLEALTDVKANTLTDWVAQPQVARTIAREFKSFLTEYTDEQGHSVYGTRIRTLGEINAESLEVSFDHLAEQKATLAYWLANTPTEMLKIFDQVAMEVVLLHYPDYERIHSEIHVRITDVPVQYTLRQLRQSHLNSLVRVSGVVTRRSGVFPQLKYVKFDCTKCGVTLGPFHQDSNVEVKISFCQNCQSRGPFTVNSERTVYRNYQKLTLQESPGTVPAGRLPRHREVILLWDLIDSAKPGEEIEVTGIYRNNYDAALNNKNGFPVFATILEANYVVKSHDQLAGFRLTEEDEKEIRRLSKDPRIVDKIINSIAPSIYGHTDIKTAVALSLFGGVSKEAPGRHSIRGDINVLLLGDPGTAKSQILKYVEKTAHRAVFATGQGASAVGLTASVRRDPMTSEWTLEGGALVLADKGTCLIDEFDKMNDQDRTSIHEAMEQQTISISKAGIVTTLTARCAIVAAANPIGGRYNSTIPFSQNVELTEPILSRFDILCVVRDTVDPTEDERLAKFVVNSHGRAHPLVNSAYGYSDKSKATQNGDEESMEVDENAPVKEGEIPQELLRKYILYARENCKPALYQIDQDKVARLFADMRRESMATGAYPITVRHLEAIMRIAESFCKMRLSDYCSSLDVDRAIAVTVDSFVGSQKVSAKKALARAFAKYTLNRPGAVRAPTRGGRQAQRAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YED3</accession>
    <name>A0A9W8YED3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003351</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YED3"/>
    <dbReference type="OrthoDB" id="630895at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007344">
        <property type="entry name" value="GrpB/CoaE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34822">
        <property type="entry name" value="GRPB DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G01530)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34822:SF1">
        <property type="entry name" value="GRPB FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04229">
        <property type="entry name" value="GrpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="192"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="402" mass="45323" checksum="442C4A041877A547" modified="2023-11-08" version="1">MSVVVEQYNSEWPQQFQSIKSELETYLKDIEYLSIEHVGSTSVPGLAAKPIIDIDIIVTRGNLQPTIDALIANGKFDYLGELGIVDRHVFKDPNQSTRHNIYVCVDGVAQTRNHLGLRDTLRRDSELRDEYARVKLDLAAKNTNIIDYLEAKSVIIQKILRASGTLTKPELAAIEKANLKGERLGAIQTPRLLLREFETKDIDSYYELESNPENARYQDWPPRTKDEARQLVLENIRNHNAVPRTVFELAVDHTGHFIGRVGAKLTPTSYDKLPGEMTLKHVTHANLCFSFLPSAQGQGFATEAMKAFIAVLTKGTRGAKDGGKWELEIECDPRNTGSVRLAERLGFERHSLTKEKWESKGEWVDSLVFRKVVGDVVLGSRHARRVSKGESGMDRFNKQIVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEH3</accession>
    <name>A0A9W8YEH3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_001220</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEH3"/>
    <dbReference type="OrthoDB" id="310217at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF13">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="31"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="14"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4375941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="391" mass="44121" checksum="EB49FED20DCC637F" modified="2023-11-08" version="1">MPFHFSFHRKHNVSSIPEASSSPADDFPAKHKYNRRLQGGGEGTVEKWTHESTGIVLAVKVVKYDKKSVPNEVQILRDLTPHNCIIQCLGYYTQQPAPDKLSLLLDYCPGGDLFDIRGRMMKRNFGIFTESFMWSMYDQLASALAFLHEGIDTQNPLGRYNWRPVVHGDMKMENVLVKSLGSKDDWSGIDLKLGDFGMAAYYDPAHPNPSGQIGTTHNWPPEVTWETKKLSPASDVWGIAAILHELAHGFGPVVSPKMAEKKWLAENIGAPYPDHWPKVFKTNYWGAKTLRRVVPINLDTKAPLPVDARLDGPDERVERLRKDRPSPKYSDALNQCMMMALVMNPQERTGAGELLSQIEEAYADFLFQDLEIEYERSVEEAGEAGDSDEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF25</accession>
    <name>A0A9W8YF25_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Structural maintenance of chromosomes protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SMC3_1</name>
        <name evidence="2" type="ORF">N0V83_001804</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF25"/>
    <dbReference type="OrthoDB" id="3776135at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="287" mass="31624" checksum="70EE3EFD466D29A5" modified="2023-11-08" version="1">MLATASMASSMSKEDSHQATPSPTNYTFIIEIGNQRQHVGDVSEVAAGQNLSTRLSAGLYDKCDKKKTGANTCKSDPIILGLIEHSNERGTVAYLDEERKPVNVNVTSDFIPEDYAGKGALQTAFIDQIVDTFTVATSDDKNCYQFDRTSTICWFCKKKLCFGCIGQYCPEHDTRGKIVRWCNAPEYVRVSLSDAQGKEIAHMRVDLKFQGTTDQGKFDCVGIIDAVDKNARERRVGKLERVVGAALGGEQLKTRVVCESREVMGSCPNEQCLYQLGSCFKRGGVMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFD1</accession>
    <name>A0A9W8YFD1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_001317</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFD1"/>
    <dbReference type="OrthoDB" id="2241241at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015343">
        <property type="term" value="F:siderophore-iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000381">
        <property type="entry name" value="Siderophore iron transporter mirB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF87">
        <property type="entry name" value="SIDEROPHORE IRON TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="346"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="390"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="415"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="480"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="556"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="62"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4376037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="611" mass="67062" checksum="2B2640EA76D941E9" modified="2023-11-08" version="1">MRPDDGVVHDGRNGHNGTTDPYHKNPTLERDPYMSEDTASEFSEKQAGVKRIEAVSKAWTKTSLIIAYVTLLLIANVTSLEIQVTGLLTPYATSAFQTHSLISTIYVVQGVVSAVIKPPMGKIADVFGRLESFCITIFFYTIGYIQQAASHNVQTFASAQIFWAAGFNGLQILQQIFVADTTDLLNRALFSTLFDVPFLWTTWAGPEVGSAILSGTSWRWGYAIWAIILPVCFIPLAVSLYMNQRRAKKMGLDVPSPFRGHTAVQVVKNLWFDMDVFGLLLLATAISLILLPLTLAPGASGGWKNPSMIAMLVIGAVCLLVFPGWEMSKKLAPKAFFPPNLFKNRTVVAGTLIAFFYFMAFYMSVFPYFFSYLQIVQGQSVVTAGYITRVFTFSSTVASIVVSLIIKYTAHYKYYVTFGAVIYLTGMGLMLAYRTENASTGTLVGTQIAIGIGGGFLNVPVQLGVQASASHQQVAAATTVWLTLLEVGGAVGSAISGAIWSTYIPSKLQQYLPPDQVADYASIYGSILVSADYTLYPPGSPGRSAINRAYQETMRYLLIGALCCATPIVPLTFCLKNYKLDEMDQKVTGRVIGNADNRETKKRGWRFWSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG35</accession>
    <name>A0A9W8YG35_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001388</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG35"/>
    <dbReference type="OrthoDB" id="3912356at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="595" mass="67056" checksum="DDCEFCF4E76C50BD" modified="2023-11-08" version="1">MADLEPKKSGFRAMFGMRPSKQPGTRKKLSKKGSASSPAVPTLGSSIVANPQRQDSGSFTSQSIDLDTTEALLPTYEDISGFVPRNFRDSPYAAADPVALNNHTYLQPPQPAEYRRNSEGEEQYAAPLYKPQSSRSASTPNFPDVLANPTVQPGLVAKAPPAKLSKAHRATPRADDILDKDTGERKDLTDMMHAFTFNEELDIIDEKFDEVSLIYDPSKPDGTAILGSASPEIWLRVADFLSPLDVANLSSTCRTMSTRLGRYPYKLLLDPINRPDRLNFLLAMDYKLPRHLLCFPCAQWHLRIQPGLETLKPHNVLNPLFECPNRTNILLPPPRIRITDGRTLPFAFVQLARRSWEFGFEYGIQHQSLARRWKDPYSDWAHESTYHVTDAGHVLMRVKSQHYVEGGMTDAAKRLLLFTRGDYTPYFSVCSHWKNGMLTSIPKCALDHIPFVHASEQYAMGLQLREKAYGKKATGLVSLCSRCRPMRRCPECPTEYLFELKFLEDKSVRGHGPERFKQALLVTRWSDLGPARSPADAEWSAIVGENENFDSFLEIGRRAVSGVFESAFTDTIPGQRILSMNPKNLKADDVKEEWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGH7</accession>
    <name>A0A9W8YGH7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL4m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">yml6</name>
        <name evidence="7" type="ORF">N0V83_001585</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGH7"/>
    <dbReference type="OrthoDB" id="275876at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1370.10:FF:000016">
        <property type="entry name" value="60S ribosomal protein L4, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013005">
        <property type="entry name" value="Ribosomal_uL4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03953">
        <property type="entry name" value="rplD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746:SF6">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL4M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="130"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="528"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4376302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="587" mass="65805" checksum="E52D22D94A80C327" modified="2023-11-08" version="1">MRGVTHQLSRLSIRQDALCARCTVRQTALTQYNILTARKKSLSSSFARAISTSTASKVSVSSITTSTDGFVPIKPIAEQTVLATIHQFPSLEPLRFETYPANHLYLPTRRDILHRAVIYEGDMTRLGTASTKTRHEVRGSRKKVRPQKGSGAARLGDKKSPMLRGGGVAFGPHPRDFSTELQKKVYDLAWRTALSYRFRKGELIIVDNAMEIESPSPRLLGDIFKHHEKLRGRGRSLLVTLEERPLLEQALVEMDRGEQTLTWEEVDVKNLLELSRVIIERDALKNILLTHQEDLTHRSITPWHKSLVRSSPPEELESTIGWQEFRALSLTDPKKKDAARADAYESVASTRYTYAETLSAGPKRTGLTISAYDLLAEAKELRFAQMTGLPFRDYMRLTENMEASDVALSFPRIQALDYQIGVKSDLATTAAETSRQKGEELMTEIRELEVQKLEIQYEAALLAAQVHEHVAESQTLSGDEAAAEDTLSLASSERTNVDLVQLQMLEARLEVAKQKVLVAGLKRDFGGVKKAQEEVTQCTELVEGKRNEIEAWDAQMAEIEGAEEAEVAQVEEVKAEPVVEVKKEEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGI8</accession>
    <name>A0A9W8YGI8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001930</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGI8"/>
    <dbReference type="OrthoDB" id="3205825at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35179:SF1">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35179">
        <property type="entry name" value="PROTEIN CBG02620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="65"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="216"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="419" mass="47720" checksum="92FF2C23D23480AE" modified="2023-11-08" version="1">MNVDITPHDYTAYGPFIHDNYVVPPVTRADLTIAGTLFSLANIFALLAAYIAIKQTKAARRPLRSHYLWMVWLEWAASVVINIECLLYLLRIIRPSFWFYMSILLNWSIQVQLLLQIIINRIRIILDRKERGRQLIIGTAILVTWINISVFCVWIPARLQINETWVRINAVWDRVEKVLYLTIDGFLNYYFIRVVKANLVTRGLEKYNRLVRFNQGIIFVSLSMDVMIIGAMSIQNSFVYAIFHPLAYLVKLNIELSMAHLIKTVAQGNPKKDNLTFKLTFGTSPNGFRMANNNIFAEPTPTRFSLLRGLSDRTVQSLSVPDNEILKTKEFVLRSSLQQGPEQHSPKHTADASIEQVDENHVNRMSFTPRPSGDSGSATVMARPGPSGEIWDEEAIVGLSPVLLRSRSPESKHASLTSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGM1</accession>
    <name>A0A9W8YGM1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_002249</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peroxiredoxin family. Prx6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGM1"/>
    <dbReference type="OrthoDB" id="2996783at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03016">
        <property type="entry name" value="PRX_1cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1020.10:FF:000001">
        <property type="entry name" value="1-Cys peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000011">
        <property type="entry name" value="Peroxiredoxin PRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1020.10">
        <property type="entry name" value="Antioxidant, Horf6, Chain A, domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045020">
        <property type="entry name" value="PRX_1cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009668">
        <property type="entry name" value="PRK13189.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503">
        <property type="entry name" value="MCG48959-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503:SF9">
        <property type="entry name" value="PEROXIREDOXIN PRX1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1 6" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="4 6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="44"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="7">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4375165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="258" mass="28494" checksum="FB1AD77350D9747C" modified="2023-11-08" version="1">MASFFPRAASRALRSATRPALNFQRPAAPLLRRGLAVPAEQPRLRLGSVAPNFQAKTTHGDIDFHKFLDNSWAVLFSHPADFTPVCTTELGAFAKLKDEFDARGVKMIGLSANDLTSHDQWVSDINETQNTTVQFPIIADADRKVAFLYDMISQDDLDALSKKGGIAFTIRSVFIIDPSKKIRLTMLYPASTGRNTAEVLRVIDSLQTGDKKGIATPINWNAGEDVIVPPSVSTEDARKKFGDVREVKPYLRYTNVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGN8</accession>
    <name>A0A9W8YGN8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arginine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Arginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SYR1</name>
        <name evidence="14" type="ORF">N0V83_002005</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Arg) + L-arginine + ATP = L-arginyl-tRNA(Arg) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGN8"/>
    <dbReference type="OrthoDB" id="68056at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07956">
        <property type="entry name" value="Anticodon_Ia_Arg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000006">
        <property type="entry name" value="Arginyl-tRNA synthetase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="entry name" value="Arginyl tRNA synthetase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956:SF11">
        <property type="entry name" value="ARGININE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="ARGINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="Arginyl-tRNA synthetase (ArgRS), N-terminal 'additional' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 10 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginyl tRNA synthetase N-terminal" evidence="13">
        <location>
            <begin position="30"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="DALR anticodon binding" evidence="12">
        <location>
            <begin position="523"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="238"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4374927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="645" mass="72441" checksum="B1EC9359B3FF45EF" modified="2023-11-08" version="1">MEDLTNTLTTLGLDKVPQEPNTYPALNPFDIYRSHITELLSQVSGVDKHIIYPTLAWTAKPEHGDLQLAVPALRQKKKDMKAFAQELADQFPESPLVKKPVVTNTQIGFFFKPETLTSLVLPMVLKSGPSYGFNKNLGLKNPLDPSSGSKKMIVEFSSPNIAKPFHAGHLRSTIIGGFLANLYEGAGWDVVRMNYLGDWGKQYGVLAIGFDLFGNEEELVKNPIGHLYDIYVRVSGIQHEEAEKMKALKAEAAKLKEEGKDSSAQEAEIKKIEAEGIDEQARKYFKGMVDGEPKAIGIWKRFRDLSIEKYKQTYARLNIHYDDYSGESQVKDESMDLAAKIMWEKKVCEDSEGAVIVDLTPHSKKLGKAVVKKKDGTSLYLTRDIGAAIERVDKYHFDKMIYVVASQQDLHLAQLFKIEELMGRKDISEKCQHINFGMVLGMSTRKGTAKFLDDILRDVGDKMHEVMKGNQSKYEQVQDPEKTADTLGISAVMVQDMKGKRINNYTFDMDRMTSFEGDTGPYLQYAHARLCSIYRKAVTADPSLAQLDLTSADFSLLKEPHAIELVRQLASWPDTFTNTLKTQEPTTVLTYLFKMAHALSSSYDHLQVIGSERNVMVARLALYVAARQVLNNGMKVLGLSPVERM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGR9</accession>
    <name>A0A9W8YGR9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGR9"/>
    <dbReference type="OrthoDB" id="5428890at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="321"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="374" mass="43195" checksum="FCEFBDDBF262A415" modified="2023-11-08" version="1">MTSTNERLFSKSVYTDLVRLPTQRKCHVINTFWPHLKLDEEAYIQEDYSDLFEHIGRVLSSLHQHETKFDDKFLDSLLATLTELTAHRISTKEALVTKIKSKYSDARDDAVARSVELTARLWLGVHVSSRQLSVGPINPRDTRIEWPNDQPLDQIVAAQFRQRFLRAVDDEYSLDDSFTVVNLKAICRIHIRWTDNLVDHLKLEGPRGHRILSIYRHKICLVNHLKGPDPTVIERGVLDEAIRTLDLLFPFGDLKTAEFLKAERVHFHSMPPSEFSQPINLAEFTYWRNNLSQLLRLLNGPPETIAQTLLDRRNLSQFATLWVAIFGVFFLTILFGILATVYSIKQYRIAVRSYELALVLACREDPTIPGFCQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH31</accession>
    <name>A0A9W8YH31_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_000709</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MSOX/MTOX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH31"/>
    <dbReference type="OrthoDB" id="2219495at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050031">
        <property type="term" value="F:L-pipecolate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004657">
        <property type="term" value="F:proline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008115">
        <property type="term" value="F:sarcosine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045170">
        <property type="entry name" value="MTOX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961">
        <property type="entry name" value="PEROXISOMAL SARCOSINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961:SF46">
        <property type="entry name" value="PEROXISOMAL SARCOSINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="6">
        <location>
            <begin position="13"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4377879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="444" mass="48836" checksum="24634A767E07D7F5" modified="2023-11-08" version="1">MASSAPVEIPSSILIVGSGAFGLSTAWALCQNPQYKNTLITVVDRQPFPCPDCSSIDTSRIIRPDYAAAPYTRLATVAQQRWRTDFAPEHYYEVGLAVTAWGPEQRYVSESLANVNAIGADKTEVLNSAEDIKRACGLEGKDEWGSGSTGYVNWSSGWANAEGAMMWLRENLEKLKRVKFVTESVKKLLIDHKMNTVYGVRLSNSTELKADLTVLAAGAWTAALIDLRGICKATGQALCYMPISAEEEALLSRRPTILNLSHGLFMIPPANGLLKIARHGHGYLNPTTIPNPESEDPHDTITTSLPYTCVDDPSQVVPTEGQRQCRDFLTAIHPSLSTPSRPFTRSKICWYTDTRNGDFLISYHPKYKGLFVATGGSGHGFKFLPVIGESILECIEGRTPEDFKGRWEWPAERVPEELWKGDGSRGGPIGLVLKEEMAKSKGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHE4</accession>
    <name>A0A9W8YHE4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">C-4 sterol methyl oxidase</fullName>
            <ecNumber evidence="6">1.14.18.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ERG25</name>
        <name evidence="6" type="ORF">N0V83_000236</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.18.9" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHE4"/>
    <dbReference type="OrthoDB" id="1658724at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000254">
        <property type="term" value="F:C-4 methylsterol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="5">
        <location>
            <begin position="133"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4377411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="292" mass="34559" checksum="863DA2C1EF07ADC5" modified="2023-11-08" version="1">MAQDTYWGSFEEISKYNVQLNYFEKMWLAWYTWMGNDVLATGIMSFAIHEGLYFGRSLPWIIIDAIPYFRRYKIQQHKIPTAWEQTQCALLVLLSHFTVELPQIWLFHPMCQYFGLETNVPFPSLFKMAYQIAIFFVMEDAWHYWMHRAMHASPFLYKNIHKIHHQYSAPFGLAAEYASPIEVMVLGFGTVSVPIVWCAITKDLHILTMYAWIVFRLFQAIDAHSGYEFPWSLHHFLPFWAGAEHHDVHHEKFIGNYASSFRWWDFVLDTEAGAEASKKRRERKLAKARKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHN5</accession>
    <name>A0A9W8YHN5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000532</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CoA-transferase III family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHN5"/>
    <dbReference type="OrthoDB" id="5863171at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047369">
        <property type="term" value="F:succinate-hydroxymethylglutarate CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1540.10:FF:000005">
        <property type="entry name" value="succinate--hydroxymethylglutarate CoA-transferase isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="entry name" value="Crotonobetainyl-coa:carnitine coa-transferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="entry name" value="formyl-coa transferase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050483">
        <property type="entry name" value="CoA-transferase_III_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48207">
        <property type="entry name" value="SUCCINATE--HYDROXYMETHYLGLUTARATE COA-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48207:SF3">
        <property type="entry name" value="SUCCINATE--HYDROXYMETHYLGLUTARATE COA-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="CoA-transferase family III (CaiB/BaiF)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="394" mass="43197" checksum="3D347DCA38277DA2" modified="2023-11-08" version="1">MTRVLAGPYCTQILGDLGADIIKIEHPIRGDDTRAWGPPYAQYTDDKEGPGESAYYLGVNRNKRSIGLSFQHPAGVDILHRLVKECDVLVENYLPGSLAKYAMDYDTVSKINPSIVYASITGYGQTGPYRNRAGYDVMVEAEMGLMHITGARDGPPVKVGVAVTDLTTGLYTSNSVMAALLGRLKTGRGQHIDVALSDCQVATLANIASSCLISGQRDTGRWGTSHPSIVPYKAFKTSDGDILLGGGNDRLYGVICNKIGKPEWILDERFRTNALRVKNRDTLEELIEKETQQKSTQEWLEILEGSGMPYAAINDVQDTLNHEHVRARDMVKDVEHPACGPIKLVNTPVKWSESKPGIRLPPPTLGQHTDEILGDILGMDREQVQNLRTEGVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YI54</accession>
    <name>A0A9W8YI54_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000788</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YI54"/>
    <dbReference type="OrthoDB" id="5396420at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="29"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="398" mass="44486" checksum="09D28788C69B26BC" modified="2023-11-08" version="1">MSIFRASTPSSPIIVYLPPGPVLPDCLEEEERVISTLRESSSATIVRVNYRASSLHQYPTPCHDVLAGYDWVRENLLRDEFQRPYLAQLGVCGQLVGGSLATMLALTECRLGESRIGAAAVNNPIVDWIFPDDLPFVAPSELPEPAAPDETAFPADEDMGSSYATRENVEKESKTVRKRQKRPPKALPTTAWVAHSDNTTLPAVVLSGERDVLFRRPEDYFDRFASPIHFFRSPHAQLIFPQHEDVAASQQPDEVVDLETQMELDHYASLNNAMMAPALPTLARCRSYARNYPPAGTKLGLPLWNITYGLHSPLSDQATELAKMVRRSIARQTLKSHSGRTRWHDATEKGQYGQLAQEQVLLSACEGVGLWTQQEDNPNWKTNVQDMGAWMKQHLVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIT5</accession>
    <name>A0A9W8YIT5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Golgi apparatus membrane protein tvp18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TVP18</name>
        <name evidence="9" type="ORF">N0V83_000496</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Golgi membrane protein involved in vesicular trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TVP18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIT5"/>
    <dbReference type="OrthoDB" id="5591789at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019365">
        <property type="entry name" value="TVP18/Ca-channel_flower"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13314">
        <property type="entry name" value="CALCIUM CHANNEL FLOWER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13314:SF2">
        <property type="entry name" value="CALCIUM CHANNEL FLOWER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10233">
        <property type="entry name" value="Cg6151-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01077">
        <property type="entry name" value="Cg6151-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4377668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="148" mass="16242" checksum="028E6D6594CF8D73" modified="2023-11-08" version="1">MTLAEEFRSRNFSIYGQWTGVLCIFLCFALGVANIFHANFVIAFSIVCLVCSFIIIFIEIPLLLRICPTSAKFDTFIRRFSSNYMRAAIYLAMSAIQWASIAAMATSLIVAAVFLLIAAIFYALAGVKGQEFQGSKTLGGQGVAQMII</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJ89</accession>
    <name>A0A9W8YJ89_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-dependent DNA ligase family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_000768</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJ89"/>
    <dbReference type="OrthoDB" id="2160351at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903461">
        <property type="term" value="P:Okazaki fragment processing involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08039">
        <property type="entry name" value="Adenylation_DNA_ligase_Fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF12">
        <property type="entry name" value="ATP DEPENDENT DNA LIGASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="6">
        <location>
            <begin position="394"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="697"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="697"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="727"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="782"/>
            <end position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4377938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="874" mass="99272" checksum="49E8E5CF26469057" modified="2023-11-08" version="1">MTITFGAICSLLQSVENISTRQPRPLPQQQQDGIRQIISNWFNNQRSALDDPLTNGGAVLSSLFPHRRKDRVYGLQAPLLAKKLTKLLAFNHGQRALFDGWQTGIHGDLGAYTERAMRPWDGTFGSKRTVTIDRIDQLLVQLAARYRFSDEAIRRQRKWHVDTDTELKEIFIRLESWEAKWLVRLILRDYCTVELEEKYVFEQYHFLLPDLLMFQNDFDTAFNMLKGDLSCYPPVPDLSKTNEMRVEAAQKLTAVVGVKVGRPTFLKAWSFANCFKLVCRQAWIAEVKYDGEYCEIHIDIDNRGKEIKIFSKNGKDATADRESLHSTIRDALRIGRPGCLFKKKCIVLGEMVVYSDREKKILPFSKIRKHISRSGSFLGTDQDSLPHEWEHLMVVFFDVLLLDGKPILRHCLQDRRKILRDLVHIIPGRSMRSAWTLLDFKGDGITDLKQAFARNLADRQEGLVLKPLHAPYFPLHSGQDYRYAGFFIKLKKDYLGDMCGERDLGDFAIIGASFDPQVAPKTDLKPLHWTHFYLACCTNKAAVQRKGQKPRFKIVATLSLDKCIPKPDVKYLNIQGYLRQSVLHKHGSPDEYDIEQPKNYGRRMAVAFKKPFVAEVLGGGFEKVPNEEFEMLRHPRIKKIHHDRTWEEDAVTMEDLERMAQEKWGAPDAEKLNSHARDVALLVEKYVKEIHGDSQVTITTNETTQQTTPRTTQETVQHSSPEAVIQETPQHTYTTVSTSQCSGDGSTQGKGIRASRQVRILVREDTSERLAVPPSPTPKPSTLPSSAPVSAPSTGPTPSDTSSTAKKRSFDQNISPPTLKRRRTTTPLKNASGNRSLGSFDFDSQEGTIHVYAKEGLKVQVHTGTAEESSQGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJV2</accession>
    <name>A0A9W8YJV2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin-conjugating enzyme E2 6</fullName>
            <ecNumber evidence="3">2.3.2.23</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">UBC6</name>
        <name evidence="3" type="ORF">N0V83_000680</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJV2"/>
    <dbReference type="OrthoDB" id="1158011at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23799">
        <property type="entry name" value="UBCc_UBE2J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000058">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="5"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="276" mass="30293" checksum="263E53BE30CB1FD5" modified="2023-11-08" version="1">MATPAATKRLTKEYAAISESPPPYIVAHPSDKNLLEWHYILTGPPDTPYDGGQYWGTLMFPPDYPYAPPAIRMHTLSGRFQPSTRLCLSISDFHPKSFNPAWGVSTILTGLLSFMISEEMTTGSVRASEQERKLFAQRTRWWNSTGGGSKQTLAQSSNSRGIGGIKAGDGGAKFRLEWPEVDEENLKWMKEKHIDSATGLPRPVQTSSQPHCSPEVAGLRRRPGASATVVHDAAQIARDAGQGWLSRNKWKIVFGAMMVYIFVARVLGDGAVSSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIX1</accession>
    <name>A0A9W9CIX1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">COPII coat GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SAR1_2</name>
        <name evidence="8" type="ORF">N0V83_007739</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIX1"/>
    <dbReference type="OrthoDB" id="2988756at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF166">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="19"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4366104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="203" mass="22551" checksum="BB1F9D5A9EEDC05A" modified="2023-11-08" version="1">MVVLDLCRRMVRKLSYEFTVLYTFWTVIACTYIAATIAIFVECRPCNRYWQLSPDPGECVKGNAWLLTYEVGNIITDSMLLALPLPPLFHAKTTLSKRLRLLSLFSLGFFLIAVSIVRMVQGNKNARIQLSRTMWASIETLFASVVAMGPAIHGLGGGTVESRAWTELRNLGRQDAEDASTKGILAVTTVTHMQESDLGFSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJB0</accession>
    <name>A0A9W9CJB0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Mediator complex subunit 18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">srb5</name>
        <name evidence="8" type="synonym">MED18</name>
        <name evidence="10" type="ORF">N0V83_008902</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJB0"/>
    <dbReference type="OrthoDB" id="5348092at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019095">
        <property type="entry name" value="Mediator_Med18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION, SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09637">
        <property type="entry name" value="Med18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4365283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="277" mass="30865" checksum="028F417B59A65B2E" modified="2023-11-08" version="1">MHELLLYGQVPFGRHEQVLKILAGVAAMQPRRIIERRIIYKPLREPEEPGSNLRRGGTQAVAVKQTKQTPAPSLYYTKLVQKLLEDEFGTEDGLPHEDGKSLSADVADGDDPTWSHLFEDLPDTGDRGVSIRFSNSTDFLSGDPHAFMIASGPNSFVTEYYVEGHRYVHGNVIIYLHRVLHEPGVRNVQTAPKVNLPSFASLDLLDPSGSYILEAKVRVLDFNNAAVSESGVDELKRFQKEMKGCVELSLPDRLSLDPRVKYKAPHAPATNAQARPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJB4</accession>
    <name>A0A9W9CJB4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_008191</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WHI5/NRM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJB4"/>
    <dbReference type="OrthoDB" id="2163387at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013734">
        <property type="entry name" value="TF_Nrm1/Whi5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40468:SF1">
        <property type="entry name" value="TOPOISOMERASE I DAMAGE AFFECTED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40468">
        <property type="entry name" value="YALI0A15257P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08528">
        <property type="entry name" value="Whi5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="88"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="173"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="260"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="122"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4365571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="386" mass="41500" checksum="5DC4D85A3E8AEA38" modified="2023-11-08" version="1">MAEQDEVANKVLRRAQVSKMTRTLQNRLALANVKIKNGWESLSLDAIEPVVDVELKRKRPGSSNETMSDVSSVSERFYQPGVLDSSPLAAPMFSDDLRRSGSRSGSSHSQNKRPRFDRTVYPASSNHTRNKVRTATSASASWKNSRQLPESSPVYHHRHARFGRTHVPSLSFVSESSTKADSHSPLLSEEDDEDLPITSFQINKSSRFRSSPPRAPRTPPPDVARSARLRQSGFTAAGAAREGKTGNDDASLLMYLATSPTPARPGSAKPQMLAPSTPPMKQTPLPSSMMSTPGGGGAPFSGFGLATPSTNLNFAEFLNMTPSPAQVGASWSRTPIGGKTPAAAREARRRLNFDNLVPPAGESPRLGGRIGKVEGLGMDLGGELVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJH6</accession>
    <name>A0A9W9CJH6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">L-ornithine N(5)-oxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_008506</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FMO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJH6"/>
    <dbReference type="OrthoDB" id="2915840at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="432"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4365884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="611" mass="68328" checksum="6BF2FEB230543DD5" modified="2023-11-08" version="1">MEEEADLVIIGAGVYGLTSASTYHRLHPEAKILILDSSPTIGGPWAPHRIFPGLNTNNLWGMYEHPDFPMDSEKFGVKKGEHVPAQKMFEYLQAFAEWAGITKFLRLSTAVEVIERDGENWALHCKSWASSSSESESSKLLLRTKKLIIAVGATNKPFMPRYPTSPSFTPPIIHSKDFPAHFNAIVKPNTHTLIIGSGKSAWDIAYASATATADPSSTATLLIRPSGNGPMWMTPSHVTPLTLWLEKLVFTRFFGFMSPCPWASPNPYSIESILRWFFQKTWLGRKVTGAFWKILGDDAIALNGLMEHDETRKLRPWRGAFEVGNGLSIHNYPANFFDLVREGRIKVVIDEVERFGEGRDVFLKSGGDRIETDAVVCATGWQIGNTFTIRPASLERDFGMPSMRPLSTSEAEAIRSTEEQLYKAFPYLRERDTSRSHHPDPRLRYAQGKEKDEEEGRARQPYRLHRFIAPPALLPTRSIAFAGALMCLGTFPCAYLQSLWITSLFDGSLALPSSPSSFPSSPSPTSSLSTAFLGHQQIHHEMLRDTQYCVLRGAMSYGHTLPDLVFDSLPYFDVLLADLGVRGGRKGGWWKGLGSVWCLMDRRIIVGWLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJI9</accession>
    <name>A0A9W9CJI9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_008312</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJI9"/>
    <dbReference type="OrthoDB" id="167398at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043020">
        <property type="term" value="C:NADPH oxidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016175">
        <property type="term" value="F:superoxide-generating NAD(P)H oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042554">
        <property type="term" value="P:superoxide anion generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000103">
        <property type="entry name" value="NADPH oxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000004">
        <property type="entry name" value="NADPH oxidase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050369">
        <property type="entry name" value="RBOH/FRE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972">
        <property type="entry name" value="NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972:SF153">
        <property type="entry name" value="SUPEROXIDE-GENERATING NADPH OXIDASE HEAVY CHAIN SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01169">
        <property type="entry name" value="NADPH_oxidase_subgroup_(NOX)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="174"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="12">
        <location>
            <begin position="270"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4365692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="560" mass="63876" checksum="5B6CA698F233B592" modified="2023-11-08" version="1">MSSYSSLTGRERSRWPPLTRMLMSGEMSDEKGRQLTRKEKFDVWMVNEGYRRAFVGTFIILHIMVFTFGMMNYGLKDSSIGSRNSFGFTFPVARSAALVLHVDVAFILFPVCRNLISMARRTPLNDVIPFDKNITFHKLVAWSIVFFSWVHTIAHWNNLARFSAANNLGFMGFILANLATGPGWSGYVMLIALMAIALTAAEKARRANFERFWYTHHLFIVFFIFWSIHGAFCMIKPDFAPFCDGIGVFWEYWMYGGFAYLAERISREIRGRHKTYISKVIQHPSNVCEVQIKKEHTKTRAGQYIFLCCPEVSIWQYHPFTLTSAPEEDYISVHIRMVGNFTKAFGKALGCNIEKKPAGEKGEKSGSGEEIALRQILPRVYIDGPFGSASEDVFKFEVAVLVGAGIGVTPFASILKSIWYRMNYPQGRTRLRKVYFFWICRDFGSFEWFRSLLLAIEAQDTEDHIEIHTYLTAKIKADDATNIMINDANAEQDAITGLRAPTNFGRPNWDMIFKSIRKIHSPAEAGVFFCGPKGLGSTLHVKCNQFSEPGFSFVWGKENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJP9</accession>
    <name>A0A9W9CJP9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJP9"/>
    <dbReference type="OrthoDB" id="4489171at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055335">
        <property type="entry name" value="Ucp6/RUP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39597">
        <property type="entry name" value="UBA DOMAIN-CONTAINING PROTEIN RUP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39597:SF1">
        <property type="entry name" value="UBA DOMAIN-CONTAINING PROTEIN RUP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="322" mass="35215" checksum="C96B0AAFB75142CD" modified="2023-11-08" version="1">MTELDEQTAQIQKKIDGISRRFKPAVDDGADILIDTTDITYPEGQSPEDAMHHPYRLYGVATRHDVVYLLHPDITSNSSGAKQWWRMQYDTESSNPIIRRDRLCLQEVIERATSESASALLIYANDAATSVDPLPLSKELEEFVKKDKLAFLEELQKGATATAGATSGWENYYDGGYGNIARGGWDKESDPDGTIAGSAPPSYDDYEWGTMSAKQFHDSHRERSDETMSSTTLTPNTEVGDDDGRGVKQMVEVNGGMDAFSGLASSTASSVTVGGDDTAEAMDVDGASFMNVDMHIPEVRDDMDTQHIEVATPLEPVEKKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK47</accession>
    <name>A0A9W9CK47_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_007584</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK47"/>
    <dbReference type="OrthoDB" id="5404335at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4367054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="305" mass="35137" checksum="AF45992574C20B07" modified="2023-11-08" version="1">MGAYIGYPSGMFLHGLVPWCMMETRQLLEQNDRLGQITKERKVISISERVQDLALDLGFSFCDQREHMPFLSRKPAYSWLTSRFSAEYNSLEKGITESISSSRPQGPRTVYNAKALLLPWTFELLKDYRPLDWSRRFQRRCRDTHKSHFHPCKLLATQLIILDMGIRLQIKGSARMIDGRVDQGIELQDIDLDQDFSRSLILEAPCIVAAIVNSHTLLRSVPSQTATDVKDQELKYQPWDLSGALRRHCTFYDNDMEPNEAAIEYERLAIMLKLRALFFLAFLMIGPDTSSVYKARESQAQVPII</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKR9</accession>
    <name>A0A9W9CKR9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab proteins geranylgeranyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">MRS6</name>
        <name evidence="3" type="ORF">N0V83_005946</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Rab GDI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKR9"/>
    <dbReference type="OrthoDB" id="1923006at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.405.10:FF:000003">
        <property type="entry name" value="Rab proteins geranylgeranyltransferase component A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.519.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018203">
        <property type="entry name" value="GDP_dissociation_inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017230">
        <property type="entry name" value="Mrs6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787:SF4">
        <property type="entry name" value="CHM, RAB ESCORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787">
        <property type="entry name" value="RAB GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00996">
        <property type="entry name" value="GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037514">
        <property type="entry name" value="Rab_ger_ger_transf_A_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00891">
        <property type="entry name" value="RABGDIREP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR037514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="513" mass="55503" checksum="E9E9083B6DA93210" modified="2023-11-08" version="1">METLDNTEWDVLIVGTGLQQSLLALALSRSGKKILHIDENDYYGGAEAAFSLREAEEWAQRVNDGTSSGAFSNVSIAKPETTESDPSAPALSVSRAYSLALSPQLIYARSSLIQYLVSSRVYRQLEFLAVGSWWVYSAGADSEASATSGDASRHGRLSKVPNGREDVFQDHDLDFKAKRSLMKFLRFIGEYEEQTELWEEHRRQPFPAFLSEQFKVPATLQAPLLALTLSPSSSGQTTTEYALPRIARHLRSIGVFGAGFGAVIPKWGGLSEITQVSCRACAVGGGVYVLGKGLSTASDSVFESTETGVKLSLKDGEAVTAKWVVGGNTTTTEKDTCCRSLTIVSSRLTALFPPIAEEAPAPACAVVIFPSGSLLLEEQVEDLPSVQVFVHSSDTGECPSGQSLLYACTSLSGEVGFKLLQQAVDALLSSVDVTPPPHILWSMRYQQQSSSGPESLPIAAGYRILQFPPPSMDLAFDDAVLDKVRELWQKIVSDDGGEFLVFQDREAYADADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLE4</accession>
    <name>A0A9W9CLE4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006744</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLE4"/>
    <dbReference type="OrthoDB" id="3932329at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="253"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="414" mass="47138" checksum="8EECDA60ACD3F1B1" modified="2023-11-08" version="1">MGNTPSSRGEDSNWKSDARYAMESGVFCVICGGPFDMEGDVYNIDPKEERYRWLFNFRLLGSPEHVLEHKVTSEEVDLINTSRSEDVFLSEPAWFSMTGCGYIRVGGDAYGGDAWFDALCDDSDADTLFPLHDVCIKISCRIIDNSKANRTDIAQLPALTVLNRVLQTRFRANAHAPFEMRNDILDLCAIWEEYLPRSVIGLTKLEWWGGEYEKYYANPLNVPDITSFALGVLKASPNRKHFDDCIPATTRAPHSLERLPPELLDQICNYLPATSVITLHRVSKTLTHNVPLDNTFWRNSLLNGSLLPHIWDFDKSELGTPAFETFSDWKIVAKLLAERRFPISGRDRRLEDIPNGLWNRCRIWSIMEEAINLYFLDTPHKHGSNGAIDIPYQHSLPEHDETLIFGDLDAVTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMA1</accession>
    <name>A0A9W9CMA1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="13">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">MNS1</name>
        <name evidence="16" type="ORF">N0V83_005197</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + 4 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 4 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56008"/>
            <dbReference type="Rhea" id="RHEA-COMP:14356"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:139493"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) + 3 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 3 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56028"/>
            <dbReference type="Rhea" id="RHEA-COMP:14358"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:60628"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="13"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMA1"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF55">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MANNOSYL-OLIGOSACCHARIDE 1,2-ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13 16" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 13 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="686"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="686"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="402"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="526"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="578"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="666"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="469"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4370676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="718" mass="80293" checksum="D1A680144A668631" modified="2023-11-08" version="1">MYSTRDPFNLRSKASLASPTAFSLLQNAANHTASRALESSKQVVEATKQAIQDTDMSFAVPRNVPNFESAQRRFEDNVWNKFAGNEKGLPMYKDKPTGYGYGGGARGRKGFMRSKRGMGLIALVVLGLFYWFGWSSSGTKVGLDGEKKESGSSWSPISGGGTGGKKEKVDWEQRREEVKNAFLLSWNAYEEHGWGYDEYHPVSKTGRYMAEPNGMGWIIVDALDTLMIMNLTKEIKHAREWISTTLDYDKKQDVNTFETTIRMLGGLLSAHYLQETLPGLKPENGNEEDLFLEKATDLAGRLMGAFESPSGVPWASVILKDGKGEASHADGGASSTAEATTLQLELKYLAYLTGEAQYWEKAEKVMEVVDNNGAKDGLLPIFIYADKGTFRGNEIRWGSRGDSYYEYLIKQYLQTQKQEPVYRDMWEESLAGAKKHLLTYSKHSHFTVLAERPTGLEGHLHPKMDHLVCFLPGTIALAATGGAPLSEARKQPGWGKLQEEDMQLARELTKTCMGMYKVTATGLAPEIAHFELDDPPKMYRTEILASKSNLEVDVPAGEGWKADFNIKVADAHNLQRPETVESLLYMWRITGDEVYREWGWEMFEAFVKHTIVENGGGFSSVGNVNTVPPPSRDNMESFWLAETLKYMYLLFGPNDLLPLDQIVLNTEAHAFPRFDASKKRFKTGWERIPRDGKGNLVPKKEVGKGGEKEEVTTTTSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CME5</accession>
    <name>A0A9W9CME5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">nitric oxide dioxygenase</fullName>
            <ecNumber evidence="4">1.14.12.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">N0V83_004661</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">In the presence of oxygen and NADH, it has NADH oxidase activity, which leads to the generation of superoxide and H(2)O(2). Under anaerobic conditions, it also exhibits nitric oxide reductase and FAD reductase activities. However, all these reactions are much lower than NOD activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>2 nitric oxide + NADH + 2 O2 = 2 nitrate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19469"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.14.12.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2 nitric oxide + NADPH + 2 O2 = 2 nitrate + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19465"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.12.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.12.17" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CME5"/>
    <dbReference type="OrthoDB" id="436496at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008941">
        <property type="term" value="F:nitric oxide dioxygenase NAD(P)H activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071500">
        <property type="term" value="P:cellular response to nitrosative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046210">
        <property type="term" value="P:nitric oxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08922">
        <property type="entry name" value="FHb-globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06184">
        <property type="entry name" value="flavohem_like_fad_nad_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.490.10:FF:000003">
        <property type="entry name" value="Flavohemoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000034">
        <property type="entry name" value="Flavohemoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000010">
        <property type="entry name" value="Flavohemoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009805">
        <property type="entry name" value="PRK13289.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396">
        <property type="entry name" value="FLAVOHEMOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396:SF3">
        <property type="entry name" value="FLAVOHEMOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00410">
        <property type="entry name" value="PHEHYDRXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0216">Detoxification</keyword>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Globin" evidence="17">
        <location>
            <begin position="4"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="18">
        <location>
            <begin position="151"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00056398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ4371444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="423" mass="47201" checksum="5DD58B85EEB9BEB5" modified="2023-11-08" version="1">MGAALTPDQVAIIKATVPVLAQHGENITTKFYADMLEAHPELKNIFNQTHQATGHQPRALAGALYAYASNIDDLGKLSPAVELICHKHASLYIRPEHYDIVGHHLIETMKAVLGDAATPEILNAWGAAYWQLADIMIGKEAQMYEETSYWKDWEEFTIVKKEKESEAITSFYLEPVNPEIKTKLPIFKPGQYISVQVFVEELGDGGVYQARQYSLSDAPGKQYLRISVKKEPGVELGEAKSLTHPGYISNLLHDTKNEGDVVRVSHPFGDFFFEEKEDEKDAPVVLISAGVGLTALTSIFNSLVEKKSTRPISWIQGARNSNLRAFKKNVDRIAPENKNVKTVYFSSNPIDGETEGQDYNIKGRVDLEKVDKALLYTDNNTTKYFICGPTPFMLDVENKLKTYGVPSDRVKMELFGTGGVPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMV4</accession>
    <name>A0A9W9CMV4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="6">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ARO3</name>
        <name evidence="8" type="ORF">N0V83_005126</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the class-I DAHP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMV4"/>
    <dbReference type="OrthoDB" id="4699125at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000005">
        <property type="entry name" value="Phospho-2-dehydro-3-deoxyheptonate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006219">
        <property type="entry name" value="DAHP_synth_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00034">
        <property type="entry name" value="aroFGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009395">
        <property type="entry name" value="PRK12755.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE DAHP SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225:SF18">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE, PHENYLALANINE-INHIBITED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001361">
        <property type="entry name" value="DAHP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 6" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP synthetase I/KDSA" evidence="7">
        <location>
            <begin position="75"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4370605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="393" mass="42943" checksum="C530042E7D4F62E8" modified="2023-11-08" version="1">MSVSNFRHDSRHQLFSNSAVQFHIENPAVGDPHNSEDYRIRGYNPLTPPDLLQSEIPQSAKSRETVIKGRDEAVAVVQGKDAQRRLLVVIGPCSIHDPAAALEYCDRLMKLKEKYQNDLLIVMRSYLEKPRTTVGWKGLINDPDIDNSFKINKGLRISRQLFLDLTEKGMPLASEMLDTISPQFLADMLSLGAIGARTTESQLHRELASGLSFPVGFKNGTDGTLDVAVDAIGSVKHPHHFLSVTKPGVVAIVGTIGNDDCFVILRGGKKGTNFDAKSIKESREKLESKGLNARLMVDCSHGNSEKNHKNQPKVAHVLAEQIAAGEDAVMGVMIESNINEGAQKVPAEGKAGLKYGVSITDACISWDDTEKVLEELAQAVAKRRSLLGVNGHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMZ0</accession>
    <name>A0A9W9CMZ0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004392</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMZ0"/>
    <dbReference type="OrthoDB" id="410198at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052523">
        <property type="entry name" value="Trichothecene_AcTrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791">
        <property type="entry name" value="GNAT FAMILY ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="71"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="255" mass="29235" checksum="135716D14B5C3B6E" modified="2023-11-08" version="1">MDLEWLPTEAKPSDSHEIASLFAQSWTSPFTQLQFGPLEPHILAKTMAPRISRQMGMPNVRFDVIRHPTTQTIVSVAQWTLPSSEEQQQQQGEEDEEGTAASPHQETQEQNNEHQLFEDESYVNTLPDECNKSLILAFTIGLRNLRQQVLQNQKKYYSLENLATHSDFRGKGLARRLIEQVLAQADDKNDDVLVYLETASDNLTAMGLYKSLGFEERGRYRIEDLREFVGREELERCGGVTEHTHVAFVRYPKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN10</accession>
    <name>A0A9W9CN10_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Srp40 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004453</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN10"/>
    <dbReference type="OrthoDB" id="5599646at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039191">
        <property type="entry name" value="Nopp140-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007718">
        <property type="entry name" value="Srp40_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23216">
        <property type="entry name" value="NUCLEOLAR AND COILED-BODY PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23216:SF1">
        <property type="entry name" value="NUCLEOLAR AND COILED-BODY PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05022">
        <property type="entry name" value="SRP40_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Srp40 C-terminal" evidence="2">
        <location>
            <begin position="356"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="196"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="308"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="425" mass="43695" checksum="4E6290BDDD26176F" modified="2023-11-08" version="1">MATDKKASKKPNGDKKQKKTTPTNDARDTKTSTIQAPLHLVQSFLEQQGEKAAASKLQEFEQWEKSGATNKAQDAPEPMDVDEESASSSASSSGSSSDSSSDSSSDSEVEQKPTKKPTAKAKVSVKKAASDSSSSSSDSSSSSSDSDSDAEVASVPAKVKKAASVSSSSASSSSDSDSDSDSDSDVDPANKPLPESDSSSASSDSSSSDSDSDSSSDSDSEAEKKAPVVKKVKKAASISSSSASSSSDSSSDSSSSDSEPEPKMAKKVEVKKASKKAASSASSSASSSSDSSSDSSSDSDSDSEPETAPAARKSSSDSSATLAAASPVPTAGNKRKFSGSPAADAPAKKRNHVENRFQRIKPDTQVDPKLASNAYVSYDYADRAHQDLIVTKGKGFTKEKNKKKRGSYRGGMIDTSGGKGIKFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CND0</accession>
    <name>A0A9W9CND0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cruciform DNA binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CRP1</name>
        <name evidence="4" type="ORF">N0V83_003280</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRP1/MDG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CND0"/>
    <dbReference type="OrthoDB" id="5873279at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050827">
        <property type="entry name" value="CRP1_MDG1_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343:SF81">
        <property type="entry name" value="CRUCIFORM DNA-RECOGNIZING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="3">
        <location>
            <begin position="4"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="89"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="293"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="551"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="293"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="562"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="617"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="658"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="674"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="714"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="753"/>
            <end position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4372989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="762" mass="78626" checksum="27EF3CE0E4DEE880" modified="2023-11-08" version="1">MGKYTFTWEHSAHEVFVTGTFDDWRKTVKLEKEDGIFKKTVELPKTHTQYKFVVNGNWCINESARKEDDGHGITNNVLLPEDFVDEPVHTHSSAAPESTTAALAGSVPKEEKSRSDDSLVPGAFPQTPAAETPLSEAQTFNVNPLPATSGLGNPIKLAAGEPVPDPSTFTDNTVHSTVKHDKEPEEATTFGVAPIPATAGAGNPIHLAPGEKVPHPSTFTDNTVNSTAKTDPSSYEKSDALPPLAFTPQAEREAKGGMFGLPPVMGNMIPESSLPMGVDAKTEKDTGIHIQSAAPSSTTAALAGQVPKEPRGVPEAVTESQKEAGFAPEAAANSDAVQEKKEVEQELKEKVPEAPAAADNSYASNISSTLGGAATTAVAGATAGAGLFTAAVVSAKDKTAEATGLDSSATAGDVKDKAAEAVGLNSSAPADDIPDVPVRVIESQQEAHISPEAASNVEAVHEKSQVEQELLSEIPKTNETGTSAPAIAIPAIVSESQKEAHVGPEAAANTFAVADKKAMESELLSEIPKANEAGEPAPAISAATIETAPGANASHVANTESVAREVPEVVAESQKEAHASPEAATNAEAVSEKKAVETELLSEVKTTNATGEPAPVAAATTSTSAAGLSDEALVDSKPLKSSSEPDALNAPASKPAQPLTSKPIDSNTDVAFAGKEPTTTEQTKPVVTTGTETTTTEAKSDAPAAAAPAAAPETPRKDVRKDSDVSPKGTPGSQSIASGADDKKKKRRSIFGKLKEKFSSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNI9</accession>
    <name>A0A9W9CNI9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Clavaminate synthase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004817</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNI9"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="194" mass="21402" checksum="D944EBC64F426CBA" modified="2023-11-08" version="1">MSRDMQEAIDWYREWDESEKQEPENDGGPLPGAVKTLQGKNLWPERPEKLKNVYQAYIERVKKVGEALVHAMGVALDLGPPQANSDTNASSSDHEDEEDEEIFLRNCADSFWVMRMIGYPLSHLPFPPLPSSTTEADISQFSCGAHTDYGCVTLLLTDSTPSSLQVQLTDPTTNTTTWLNADPLPGPSSSTSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNQ9</accession>
    <name>A0A9W9CNQ9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNQ9"/>
    <dbReference type="OrthoDB" id="10648757at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="437"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="582"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1017"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="596"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1017"/>
            <end position="1034"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1192" mass="129088" checksum="E94C2FF35197E12E" modified="2023-11-08" version="1">MPADKPNINPSSMSPNTIILSAYNGIIKCTPDPTTPRRLSIFTRKAADKGVEVVKMNRSYVNKCIADDALEFLQHEIENLGEDIRFRAGTYKILFLPRIRPKQDKDGNHVDVFPELGFPEDCMPPGDQNILKATGDGDGDTGVVVFFGGKQHEDAELLPDDYNDKFPEVIEHDAGEPVLSGAEIPGGVNEPLKKPTASKDGEDGDEIVGPEPTDKKKSKKNKKQSKASKKLNAEILPGLEQLLAKVVAEGVRPEATSVGAASEKPKGAEKVEQDKAEKMKLLVKIFKLEDPTSTEPAQKEQVDIAEKGFYKEKAMIQKIATTNLHSDVNTTQRDYWKYAFVDMFPDMSLLECLSRVTAMEAHDATQNNQTAWDAKWHSYTHVRDAMNCKLNPKVWMPILLALSTGSERFRDVPSEIRSTADMDSVTTKLKSSVAAHATTPSGAHPTSVLLQSSPNGDASPTNMFSKVKDAVSSPKDTSLKPKTIKKSHRQVTFGKNMDLAPTAVAQITFPFSKDTFDFNVPPPPPMQNSLPAESGPSLIFGDASKVVGLPLADNVFARAALHRPGSEFVGFASGQPTASAPYVFGADSSSPPPPAKTDEELDRSGQKSLPGFEVPTSFVMPDGQVSGPSLPEATPVKEPVASSEKAQEATAVTLSPGETHENDVFALTGSVKEDADQTYFDPEEDGLSEMSEESLGEMILRDFLAEEDAPVEEDILDAETVSSSDSLDIDIDGLLDELFEDTCAPIPSGFYEYLARCDYEVNDVEEGLYVSEDELPLDLKYVNRAEDPITLPTPPTSPISSTSAIAASAQTGVLEGFSLKEPRYMSIDEIVNTTSIDPDCEPHSSSVQILSAGLGSNDTQTISPIPDPVANKGTINGLALETWSRLQGFGDEQGDSNTFDERLKKLIDGLQQLQADATTAQPDIGSSDEPSLEGVDAEILTATLYLDEHFNNDKEVHVEDSKLPSLGPLEDLEVWAHATFDNAFGSPPQLPALQSDQTDLHKLFEQEPLFQFLHTSSDGADQSTEQLHASTSVADKSGNHESTRLEQLEHMTVADLMVDTGDAAQAPKNVTAIVGGYVTEQKAAPHLRISTTATVPVGHFFDRIGNHPAFRLGEHTPPHTPMSEHAMVSSPAFYINHTHANALQSTPLSSNAATQTPPYLGVSDHIGLYDPLQPLDKKLTMSSAALPTRPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNS2</accession>
    <name>A0A9W9CNS2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNS2"/>
    <dbReference type="OrthoDB" id="3260716at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="173" mass="18087" checksum="0268D8CAB4218F25" modified="2023-11-08" version="1">MTSRNPDAVVNQGVEFHARAPPSEPLTSKGHQPGKLVGNEKVPTFEAEVLPAGSAPSKDTYQPNPELNNQKLYQDASSTLTGADSGSVHTGLGHPGQGQTSQELHKDNRKGHGLAGLAESVPDKGGISQGVESLKDLPKHAAQRNLDDVPTGQRGNVGGPPAEDREPVQADQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNV1</accession>
    <name>A0A9W9CNV1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNV1"/>
    <dbReference type="OrthoDB" id="5422320at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024526">
        <property type="entry name" value="DUF3807"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40642">
        <property type="entry name" value="YALI0F31295P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40642:SF1">
        <property type="entry name" value="YALI0F31295P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12720">
        <property type="entry name" value="DUF3807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="216" mass="24272" checksum="6F8F27FA6CB0C667" modified="2023-11-08" version="1">MAHEERLPAVNLDDLRAFHAKHFPSAPQLEHLLPDFESAPEPVQDCQNADDGGLGFYFDGVERTLDDEDVAWFRRSEIQTILRRRRQRREDGEVSEDDGAPATPTSAVDCPGSLLIGAVAPDASTLAGQSGASLVEGKDKVQSGRVEKPKQQWATSSARTKARNKKHNKNYRRKKKEERKKKEAKEAEDDESDEWDPWHQANGPDVQKEAPVDLDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP03</accession>
    <name>A0A9W9CP03_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MT-A70-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003146</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MT-A70-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP03"/>
    <dbReference type="OrthoDB" id="61116at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829:SF4">
        <property type="entry name" value="N(6)-ADENINE-SPECIFIC METHYLTRANSFERASE METTL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="479" mass="54407" checksum="502C06003DD571D0" modified="2023-11-08" version="1">MASASRLGAQSTSRPCILYQNAERDITLIDIPTSIASAQGRSDMLLLSTSPLEAPIEPKEDYRPRIQKPKATEVPDYTQYAEYKLLIQQALAEIRTHVSTPWCAPRRLPSQVPQPRGEDRAGDDLEKELESRLRDWAAWKESKGDDTALDLQKMMASLGATSEYDPMEVDSVTQQWMVSYHPAREATNGIQAAETVSPDSSEPWTCTFHNPEDVSLELSIYDIAARQARLGQDYRFTIPPRSTLFLSDSAHSDAFRTSFRQLTDEYTLPRHFDLVLLDPPWPNRSAKRKGAYEQIGGVPYLKKMLLKMDIDSYLEHNALVGIWITNKASLRQHVLGPGGLFETWNVGLIEEWIWIKTTTKGEPMFDIDDAMRKPYEIMLLGRAAPNSWTTMQHSPSVKRRVIAAVPDYHSRKPCLKELLEPYMPDTKSYSALEVFSRYLVAGWTSWGNEVLKFNWEKYWTAADDTSVSPAQQTLAIRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP51</accession>
    <name>A0A9W9CP51_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Proline dehydrogenase</fullName>
            <ecNumber evidence="2 5">1.5.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_004310</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Converts proline to delta-1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23784"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.5.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the proline oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.2" evidence="2 5"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP51"/>
    <dbReference type="OrthoDB" id="5464at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004657">
        <property type="term" value="F:proline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002872">
        <property type="entry name" value="Proline_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015659">
        <property type="entry name" value="Proline_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914:SF0">
        <property type="entry name" value="PROLINE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914">
        <property type="entry name" value="PROLINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01619">
        <property type="entry name" value="Pro_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proline dehydrogenase" evidence="6">
        <location>
            <begin position="7"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4372535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="214" mass="24245" checksum="3BDD910186C7CBD0" modified="2023-11-08" version="1">MTMAAFNISDSALVFNTYQMYLKSGFDTLKSHLQIAQARNFVSGIKLVRGAYIYVEPKRASIIHDNKVDCDNAYDAAVELLLRGKQFEGNEYPKPWTAEVMLATHNMNSVEKALNIYIQQDMSRTSITGTQKLVFAQLMGMADEITMKLASRLQAMGEKSGLGVYKYTVWGSLEDCLLYMLRRAEENQDAAARSRVTAVLMLKEIGTRLRFWRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP80</accession>
    <name>A0A9W9CP80_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SCL1</name>
        <name evidence="7" type="ORF">N0V83_003223</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP80"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03754">
        <property type="entry name" value="proteasome_alpha_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000026">
        <property type="entry name" value="Proteasome subunit alpha type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034642">
        <property type="entry name" value="Proteasome_subunit_alpha6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 4 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="6">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4372932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="254" mass="27626" checksum="A2921C57D03EA0A6" modified="2023-11-08" version="1">MSGSAGYDRHITIFSDQGRLYQVEYAFKAITAANITSLGVRGKDCAVVISQKKVPDKLIDPASVSHIFRLSSSVGCVMTGSIADARASVSRARGEAAEFRYKFGYEMPCDVLAKRIANISQVYTQRAYMRPLGVATTLISLDSEYGPQLYKCDPAGYYVGYKATASGPKTQEALNFLEKKLKSKEHADGSWEEVVELAITALSTVLSVDFKKGELEIGIVGGPKTNGQEGTDPAFRALTEDEIDERLQSIADKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPC8</accession>
    <name>A0A9W9CPC8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Annexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_001812</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the annexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPC8"/>
    <dbReference type="OrthoDB" id="37886at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000005">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001464">
        <property type="entry name" value="Annexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009117">
        <property type="entry name" value="ANX14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF102">
        <property type="entry name" value="ANNEXIN B11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00196">
        <property type="entry name" value="ANNEXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01813">
        <property type="entry name" value="ANNEXINFUNGI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0041">Annexin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="88"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4374736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="454" mass="49437" checksum="A8B274A90060FBC1" modified="2023-11-08" version="1">MQNQQYPPPQGGYPPQQGWGQQPPQGGQYGAPPPNQYGGPPQGQYGAPSPQPGYGAPPPGQYQQPPPGQYGAPPPGQYGAPPQGQYGAPPPQGQYGAPPPQGQYGAPPPQGQYGAPPPGQYGAPPQQGGYGGPPTQPSLGYGPQTAHIDASRDVEVIHKAMKGFGTDEKALIATLAKKDPMQINAIRTQYDQRLMKSLIKDLEKETSGYFERGLVEIARGPLDSDCHNLMEAMKGMGTKEVVIDDILIGRSNADINAIKARYQQLFKKSLEGDLRGDLSAGTEQMYMMIASARRNEDSHPVIPQEIEQAVTELQNSLGGFVGKNTPQVCQLLTSKNDAQLRAIAQSYEQRFHKSLDSVIKSGFSGHMEDALRLLVHRARNRAEAEAVRLEESMAGLGTKDELLVQRVVRCHWDRNFMGAVSAEFMKVYKKDLVKRIEGETRGDYERLLVACVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPM5</accession>
    <name>A0A9W9CPM5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002557</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPM5"/>
    <dbReference type="OrthoDB" id="5344169at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11392">
        <property type="entry name" value="bHLH_ScPHO4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="525"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="377"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="620"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="428"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="639"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="648" mass="70521" checksum="137EC81B53F925BE" modified="2023-11-08" version="1">MDATTEHDFGNLIDFEAANFDHLDLDWPAPDGQQMADLADSLDVHHLGHFTPQIPQDRRDGGAGGQQQAQNMGGHSMPQPTNGFFDYGIPQYSQAGTPSFTQAQDQMYRPHQHVPPTPNSIEMHGDPHRYMQQMDPQQAIFDQRYHMRKDDASFTPLVSPAVTPHDARFQIPDFTIPGAYFSPLTSPALNAQSQQHALQQSQVTPSGSSTGHSPVDVEMEMLGEPAMVHQEQGRKLRSTNKRNAPRTTNGNGRVRQSPIVKPNRRKATVSTLIPPKEVSELVQEARQNRPNPSGLDVPWSREGSETDSISPEPLLSEMRPPPKPASLTASPATMAQRNGQSTPATPASLMRIQPSPNFSGSHEALPMLEDLTLPEASLDRPTLSRNNTAIRDDGVDTPRMSARKTPKLNPLSTPGASMSGRPSPMLDPTSTPTSPAFSMTNGKRDAKLPRNSKKRNSVSSTLVSPALRPKISPSIKPLLPDGNNSSVDNTHALLLASKSNYQNILDGTTVPGVVYPTSLSTNLTSKRTSHKIAEQGRRNRINTALQEMQALLPSPLIAATDAKSPESFAQQSNNSKAAKVESAIDYIKQLKQQVSEKDDLISKKDEEMDQLRKELAALRRSDSVGSAADAAAQLKSEPHSSPSTGDET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQB4</accession>
    <name>A0A9W9CQB4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_001920</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQB4"/>
    <dbReference type="OrthoDB" id="2269373at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF85">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="25"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="642"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="651"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4374842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="747" mass="84170" checksum="233B6A4E1A0B3213" modified="2023-11-08" version="1">MAPSYPSGSSETSPESDQTIQKSFSCVLCASRKVKCDRRPGGCENCGKARVPCLYKAPPPPRRRKKGARDIDTAARLRLYEEALRQLGVDPEELVRQKSSKDAVHLDVSGINGFMENAIPDQAWKPYRSKEIGVLVSDEGKSRYLENGLWTSLQGEFREPRDLLDDSSEDELSESFSRFATEPPPIDGAGLLLGGQIPSMNLRSLHPQPVQIFKLWQSYLDNINPLVKIFHAPTVQQLISNATGDLDDVPRNVEALLFAIYSVTIETLSDGECIALVGQSKSAASQRFRFGAQHALIKAGFLRTSDITVLQAFTLFILSIQSGDARIIWILAGVAQRIGQRIGLHRDGVLLKLPPFETEIRRRLWWQIMMMEGFSQKLAGTGTAGTILMGDVEMPSNINDSDLFPGMKEMPKEHEGATEMMFFLIRCLVGQFLKRSSVGHTTFDGVWHRLTTTAVQLAIKDKAIDELEAHIDEKFLQYCDPSIPWHLMCAHLGKAVVFMMRFMAHSTDYYNVDHTPQREKDLMFDLALQVSSSQNLAYTMKEMQGFMWHVNLHFQWKSFLYLVSELRYRTEGAKVDQAWKEIEKTYEFHPSFDKDTSVKALPIAVSNLTLKAWDAYTAERGLPAKEEPYFIQLIRARRTRKKSPISRAVEPSSNGSPQLPTSMLQDSTSNLGFDISSQIAPPESFQWNTAELDVGAGLTSDIPDMVPLEYPDQMDWSKWDNLLVDFQTQDTNEVPTDLSAFDFNTLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQP0</accession>
    <name>A0A9W9CQP0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DUF590-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_001731</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQP0"/>
    <dbReference type="OrthoDB" id="296386at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049456">
        <property type="entry name" value="Anoctamin_N_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF73">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20877">
        <property type="entry name" value="Anoctamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="285"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="432"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="601"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin alpha-beta plait" evidence="7">
        <location>
            <begin position="12"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="6">
        <location>
            <begin position="170"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4376447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="727" mass="82772" checksum="32BA2771119DEEE0" modified="2023-11-08" version="1">MAKSQALQSNLDVDYVISYRFAKTDKAKAVAQFEKLCEALANVGLQTEVRNGDSHSVLLFVRVASDEHLFGEIYRSRVRDWIHGVRAAAPSKETRESLEAEPLYEAERLRIIYQLITNPRSEGGAGITPKEGEWEGVESVFALHDHAYNKDWIKKWSSQWLLTTEDLDDIRNRLGEKIAFYFAFTQSYFTFLVFPAAFGFAAWLFLGSFSPIYGIVSAVWCTVFTEYWKQQEVDLGIRWGVKGVSKIDIKRKEFQHEKTVTDPITGEQVGFFSASKRFQRQLLQIPFALIAALSLGAVIATCFGIEVFISEVYNGPLKSVLVFIPTGILTTVNPILNTLLTQVATRLTDYENYETAAAHDTALTQKIFVMNFIMSYLGIFLTAFVYVPFGNVIVPYLDIFNVAVRPFAEDEKQLHHTNSNWSINPDRLRKQVIYFTVTAQVVNLGMELIVPYLKRRGFAKFKQIQSDRAAKNGGAPPASASDPPEDAAFLERVRKEAELDVYDVTTDLREMVVQFGYLSLFSVVWPLTAVSFIINDWIELRADAMKICVEMQRPTPWRADTIGPWLDSLSFLTWLGSLTTSALVYMFWNTSTGPDGNPSNIQLWALLLTVFFSEHLFILFRMAVRIVLSKIDSPGLQKERRDRFLVRKQYFEENLSAMEKIPKMSDGGGEITRKSLEEDARLGSLHETTPETKFWGRQRGWRETASVARGFIERAQNETTVERKKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRG7</accession>
    <name>A0A9W9CRG7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRG7"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11069">
        <property type="entry name" value="CYP_FUM15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000051">
        <property type="entry name" value="Cytochrome P450 monooxygenase (Fum15)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF227">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="485" mass="54029" checksum="27B680D4CEAA00C0" modified="2023-11-08" version="1">MAIPKRALLLSSDSHFILGHFKRIYKDPSGEPQRDWIDQIPNDGILYYRNLFNHGRILLTNPKALAEVLVQRNYDFVKPERMRAGLGRLLGVGVLLAEGDEHKRQRKLLMPAFAFRHVKDLYPIFWGKSQEMSDALSAVIKNSADASSVVNIRDWASRATLDIIGVAGMGQDFDALTNPDNELNSTYRSIFAGDRYAKIVQLLLQLLPPWLAASLPLKRNDEIGNAINTIKKVARDLIRDKRAKLEKGGATDVDILSVAMESGGFSDEDLVNQLMTFLAAGHETTASATVWAIYLLCKHPEAQRRLRQELRSELPALFNPNSQISSTDIDRLPYLNAVLQETMRVFPPVPLTLRETAHDTTIQGHFVPAHTTVIICPWAINTSVHLWGADAREFKPERWLGAGRANTGGAESNYAITTFLHGPRSCIGKDFAKAEFACLVASLVGRFEFELEDERYELKIQGGITAGPKGGLMVRMRDVGGVENM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRH3</accession>
    <name>A0A9W9CRH3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_001701</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRH3"/>
    <dbReference type="OrthoDB" id="2985014at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF32">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="346"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="405"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="40"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="467"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4376418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="499" mass="56460" checksum="FDE2129BC3EEF2FF" modified="2023-11-08" version="1">MATDTKDVFYDENGERESLPACDWTSEEEKKAKRKLDLIIMPLLTLGFFCLQLDRGNIANAITDDFMEDVGITQDQFNVGQQMLSLGIVLFEIPANMALYRVGPGKWLTLQLFLFGTVSTFQAFQTNYASFIATRFLLGITESGFIPGGLWTLSTWYTRSETAKRVMVFYFGNQFGQASAKLLAYGILHMRGVGGKAGWFWLFILMGGFTILSGFVLGFCLPDSFKKPCPTFLPKWKMFTEREIYILRSRVHIDEPAKSRKKKRIGLTAFKKAFTNWRMWCHFAITLCNNGPQRAFDTYAPSIVRSFGFGQLRANAMAAVGLFLQIPVSFTFSWVSDHYNRRGETVIAGLSCHMLGYIFNRIFTEINSRGVQYFGVVWTQTFGTFSHPLNITWMSLTCTDSEERALAMALVIMGANIAGIYGAQIFRNDDRPRYRRAFAVACAVLAVGLLLAIFRFVEDRLRRRRKAQNLEDSSSEDGPHSLPIVPSDDVPAPIVDSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRQ1</accession>
    <name>A0A9W9CRQ1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CASTOR ACT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRQ1"/>
    <dbReference type="OrthoDB" id="58529at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046394">
        <property type="term" value="P:carboxylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.10">
        <property type="entry name" value="VC0802-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027795">
        <property type="entry name" value="CASTOR_ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051719">
        <property type="entry name" value="CASTOR_mTORC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31131:SF6">
        <property type="entry name" value="CASTOR ACT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31131">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13840">
        <property type="entry name" value="ACT_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CASTOR ACT" evidence="1">
        <location>
            <begin position="110"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="394" mass="42917" checksum="592C0E2E37F71720" modified="2023-11-08" version="1">MDSSMTLLSANIQFLDTQLALIHIPLHLYRNFLQSILQLLLPNATRNGFDNDSGAVQPPTGWPNEHPFVNISITPVECSIVCSRTLASELFVPVFNLLDAQSKSQMAITSEDFVVMQVDGEGLDAGQRVLELTSPLALAGISIFFITTYYSDYILVPLRHQEHVVKALEERGFHFEHQTSSYVNSFHHSRKQSASSLEVQPPNTPPPTTLSELQTRTFATLKRRNIVPTVDSSIRLVQCAGRPDSTNGMNQSRNRNSMTSAADDALHLGLVKCLISQPYPKFLSLTLTDTESASLLLDNTLLPNFPPDTLLSSTGDYLTAITLDLRDLPMESTGIVCGVAGRLVGGTAGQLEDAVEMSYLSTARAGTVMVAEEELERALGALRGAENGVVSTPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSH8</accession>
    <name>A0A9W9CSH8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000824</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSH8"/>
    <dbReference type="OrthoDB" id="3793279at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="131" mass="15080" checksum="D3D9301C1CE56D16" modified="2023-11-08" version="1">MLSFSTLTDALAVNRKSTDSVQEEVAFVAPNVARGVSVDNVVERQDQVLDCPDDRWQQILDNNPPDRVMTFCNEWLGIGPATTVVEWTPTIFYNRYHFYHPSDNDANSHANDNYYYDFSNDHCPTNWSQSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXE1</accession>
    <name>A0A9W8XXE1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Splicing factor subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010884</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXE1"/>
    <dbReference type="OrthoDB" id="274726at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009846">
        <property type="entry name" value="SF3b5/RDS3-10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20978">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20978:SF0">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07189">
        <property type="entry name" value="SF3b10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="12994" checksum="D14A2BAF07AB9237" modified="2023-11-08" version="1">MADKLRAQQQLEALQARYVGIGSADTTKHEWTSNIARDSLSSYIGHPPLLQYMSIGLGQSREQTRLQLLERMIRPVGPPPEVSSDEAETSETSETPETSETSANSETTETPETTQNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXR6</accession>
    <name>A0A9W8XXR6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-hydroxyisobutyryl-CoA hydrolase</fullName>
            <ecNumber evidence="3">3.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">3-hydroxyisobutyryl-coenzyme A hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">EHD3</name>
        <name evidence="8" type="ORF">N0V83_010512</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-hydroxy-2-methylpropanoyl-CoA + H2O = 3-hydroxy-2-methylpropanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20888"/>
            <dbReference type="ChEBI" id="CHEBI:11805"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57340"/>
            <dbReference type="EC" id="3.1.2.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.4" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXR6"/>
    <dbReference type="OrthoDB" id="1737613at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003860">
        <property type="term" value="F:3-hydroxyisobutyryl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000026">
        <property type="entry name" value="3-hydroxyisobutyryl-CoA hydrolase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045004">
        <property type="entry name" value="ECH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032259">
        <property type="entry name" value="HIBYL-CoA-H"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004127">
        <property type="entry name" value="PRK05617.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176:SF3">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16113">
        <property type="entry name" value="ECH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl-CoA hydratase/isomerase" evidence="7">
        <location>
            <begin position="75"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4362419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="493" mass="54897" checksum="CB16FECCEE7B1A82" modified="2023-11-08" version="1">MSLRTLSRAPLSWASRTTTSTAPIAASSMPLRAKITSPAWRSGTRKYATPTADLLKAQESDDPDDVIFTNNYGVRSIELNRPKKLNSLNGSMARKIIPRLQEWAKSDMAGVVVIKGSGRAFCAGGDVAALAQWNKQGADGQQQSSDYFGLEYKLDHLIATYTKPYVAFMDGITMGGGVGLSIHAPFRIATENTLFAMPETTIGFFPDVGASFFLPRMEGSLGTYLALTSEQLKGVDAFYHGIATHYVHSSTLQQLESRLAELQFPDYMSLNDRFKIINATIEEFSTGLPSETPHVSGQLRKTIDRVFHADQPSISAILESLEQVQNDSSSPDLHKWAEKTIKTINERSPISVAVSLQQMRIGRQWTIAQTFQREYDIASQFMAHPDFVEGVTARLIERKKERPNWSPNTLKDVKKREVDAFFAPGKAGPLPLISDKDYKEYPHAWIGLPREEEILREASGKKGEDVVASLLEKYEGKQGVREKIKEVLDRYKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYG5</accession>
    <name>A0A9W8XYG5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Endocytosis regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LDB19</name>
        <name evidence="3" type="ORF">N0V83_010504</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYG5"/>
    <dbReference type="OrthoDB" id="3832628at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024391">
        <property type="entry name" value="LDB19_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF76">
        <property type="entry name" value="PROTEIN LDB19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13002">
        <property type="entry name" value="LDB19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LDB19 N-terminal" evidence="2">
        <location>
            <begin position="125"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="410"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="436"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="447" mass="49731" checksum="C36AE112426794D4" modified="2023-11-08" version="1">MPGRLLSTLVRVAPTPLPKLDAVKPHLQPLFPPVYSYEAPPKEKTRSQESLVMDIFNRHKHDKHHHDDHKKSPKVEPVGVASMNMSVESPPVVFYNSPQSSTGAIFSGQLMINVHEPYVSVEKFEMRLLAIVSTKKPVAPHCADCSAQTTEIHKWEFLTHPTSLRHGPHSFPFSHLLPGHLPATTHGSLATLDYYLSAVATTSSGDKITYKRSLDVRRAIFPGNEKHSIRIFPPTNLTASVKLPPVIYPIGDFPIEMRLSGIVQNKSDSQTRWRLRKLNWRIEETQKFIAPACSKHISKVGGEGKGVLHEDVRAIANEEVKTGWKTDFEKGEIDVEFNAACNVALKPLCDVDSPSGMNVKHNLIIEMVVAEEWAPLKKLSQATPTGAARVLRTQFHLVLTERSGMGIAWDEEQPPVYEDVPASPPTYVDDCELPDSTNSSRSTSFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYT1</accession>
    <name>A0A9W8XYT1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_010587</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYT1"/>
    <dbReference type="OrthoDB" id="5364946at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000027">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="3">
        <location>
            <begin position="133"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4362493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="225" mass="24581" checksum="64FE609EC4FDECEB" modified="2023-11-08" version="1">MAKDIAEKKLKKEKKDKKEKRAESDGVTKSKKDKKEKKVNADVDMADALEAELEKAPEESMVNVVDGDEEPRGALVPFAFPLADNDKEVKKILKTVKKSAKTKTLRRGVKEVVKALRKSAASAPASSDISNPAAVVVIAADISPMDVISHIPVLCEDHNIPYIYIKSRAQLGEASATKRPTSVVMIAKERQGKKKEGKDEDVAEFAEVYGDLVKLVGKASKSVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ85</accession>
    <name>A0A9W8XZ85_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Elongation factor 1-alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TEF1</name>
        <name evidence="13" type="ORF">N0V83_010886</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ85"/>
    <dbReference type="OrthoDB" id="342024at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000003">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000211">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11 13" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="10 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="12">
        <location>
            <begin position="5"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4361945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="457" mass="49825" checksum="664B920C64713B76" modified="2023-11-08" version="1">MGKEKMHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYYVTVIDAPGHRDFIKNMITGTSQADCAILIIAAGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAINKMDTTKWSEDRYQEIIKETSNFIKKVGYNPKHVPFVPISGFNGDNMIDASSNCPWYKGWEKETKTKSTGKTLLEAIDAIDPPSRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGIIKSGMVVTFAPAGVTTEVKSVEMHHEQLTEGVPGDNVGFNVKNVSVKEIRRGNVAGDSKNDPPKGAESFNAQVIVLNHPGQVGAGYAPVLDCHTAHIACKFSELLEKIDRRTGKSVENSPKFIKSGDAAIVKMIPSKPMCVEAFTDYPPLGRFAVRDMRQTVAVGVIKSVVKADKAGKVTKAAQKASKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1D5</accession>
    <name>A0A9W8Y1D5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008906</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1D5"/>
    <dbReference type="OrthoDB" id="37659at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43180">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43180:SF80">
        <property type="entry name" value="NAD(P)-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="32736" checksum="E87F8BA30AEDC4BE" modified="2023-11-08" version="1">MSLFQPNASEFETLKSTPKTIIITGGSSGIGLATATLLSTLNPSHNLVLIDLQPPPPTFSHPASNLLVHTCNITSWPSQRAGFEKAYARFGRIDCVFVNAGIAEHEDQFFNDDDLDHEGKLKEPDRRTLNIDMDAASDTTKLAIHYLRKNGAKGGEIVMTASLAGYLASAGAPLYSAAKHGIVGLMRALKQECAKLSISISVVAPAITVTPILTRNNRRMNGAPASQYAAEMAKVGVPINKPESIALAVCWLLNEGKKGNGAGIFVQADRFADLERGLAKSRESWMGREMLDLFRGTRGAVVCAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1D7</accession>
    <name>A0A9W8Y1D7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008595</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4364979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1D7"/>
    <dbReference type="OrthoDB" id="9984533at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05259">
        <property type="entry name" value="PCBER_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051609">
        <property type="entry name" value="NmrA/Isoflavone_reductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045312">
        <property type="entry name" value="PCBER-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47706:SF9">
        <property type="entry name" value="NMRA-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47706">
        <property type="entry name" value="NMRA-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="3">
        <location>
            <begin position="5"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="304" mass="33158" checksum="5FA22F8D7CA63452" modified="2023-11-08" version="1">MAINNVALIGGTGTLGAPVLKALKASEFNISVLNRQTSKSVYPKTKVITIPDDLDVDDVAQALRENQIDALIITIAGSHVESQKKLIDAAFKGGVKRVMPAEFGSCDSADDKTNEILPLMKGKKVVREYLLSLEGKERGNGEGNLTWTSLVTGHFFDYGLTCGLLKFDVKGRKAYILDGGNIKFSGSNLDFIGKAVIKILEKPDQSANKLLYVHSNHVTQLEVLAALEKVTGDKFERIDQKSQGELDVARPKMLDGDGEAREEVVAVWGVVASDWKEKEGFANTILGLEEEDLEQTIKRVIAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1X1</accession>
    <name>A0A9W8Y1X1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1X1"/>
    <dbReference type="OrthoDB" id="3553147at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF82">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26639">
        <property type="entry name" value="Het-6_barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="48"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="535" mass="60696" checksum="FDE939BD05A5D7DA" modified="2023-11-08" version="1">MAESDGWLYIYTPLKHYASIRILELLPGREGSPLACSLKEVNKNDVSYEALSYAWGAPIFSKSIREVTFDTVIPVTENLYEALQVLRSEVTPRILWIDALCINQSDLKEKGHQVVPLEIFRDAVCCLNRYLDKPAGSSFADRYPINLKKQINRTSCLFEHRDKRISKTKAETSTNSPELRQTILQCFLALTRGRICSNSRDYVYAILGLGDDHLGVIPDYSLSARQTFRNFALRCLLAGETSVLHESGFHPDRSHDEPSFAPIVTFGGTKTLPLNTSKWTFQAATNSSVKADAITDGKVSIKGVRVDTIVAVGIFTPELPNSPGEVVSRMFEYEFAEVLVRGPFRWANLAFKDWIMGSMRDRDWNDDGALYAEEEDDLNTLWAPPPYGHLLMSDLFVQLITLENRNCTKPPTTLGRFRQYWTRPNMKGRPALRNRVIFKTQTGYLGVGSRWTREGDQVVIFDGFKTPFTLRREPTGSDTDDSVWRLIGDCYLMGWMHGDYFGHEVVDELPLQTNDEGQGQKEDKKVLVKTSFVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y207</accession>
    <name>A0A9W8Y207_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Acyl-CoA dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_008739</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y207"/>
    <dbReference type="OrthoDB" id="10254877at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase fadE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF28">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10880)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="9">
        <location>
            <begin position="32"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="160"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="270"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4365121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="441" mass="48279" checksum="AA1873C5F9ADA879" modified="2023-11-08" version="1">MTGSKLSAPYGSSLPWAEPAWYTGRPSAYYKDSHRKLRDAVRKWCDENIAQHTEDWLNAGAVDPAIYSKCAEAGLLVPIAFGKSIPPQWSSSYPIIGNIPPAEWDGFHDFVLWDELIRSGGLASLFIGLVVGAPPLLKYGSPSLQRKIFPEILSGEKRICLAVTEPDAGSDVRNITTSAVKTPDGTHYVVNGEKKWITNGMFSDYFMTAVRTGPPNSGAGGISMLLIPRSSPGVLKTRKIDVIAGPLSATTYVTFEDVVVPVEYLIGQEGQGFKQTMVNFNHERLWIAFQAIRGCRVAMQDAFSWALKREAFDKTLIEQPVVRNKFGNMGRMVESLQAWTEQIIYELEVLGDEEGARLLGGTTALLKVESGMVCKYVAEECLKIMGGLGLTKTGQGARIESFYRAVPSFVVPGGSEDVLLDLGVREALKIHRAAEKAKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2M8</accession>
    <name>A0A9W8Y2M8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009042</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2M8"/>
    <dbReference type="OrthoDB" id="21416at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050889">
        <property type="entry name" value="Dendritic_Spine_Reg/Scaffold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24166:SF48">
        <property type="entry name" value="PROTEIN VAPYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24166">
        <property type="entry name" value="ROLLING PEBBLES, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="280"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="395"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="52440" checksum="C505E9601CEEB31D" modified="2023-11-08" version="1">MTSNIDSFLKTREIFLEAAADLLKRPEIGAPQIVTESQMFLPCRFARFPAVVSQITKQGKPDCLGRSVAHIIYDAGEWPPTHVDSCDILGRTVLYLACLNRDFKGVKRLLKADAELIKTRLAQVLPLRPQIFSKLEVKAANGLRPLDVAVIAGDTTICKEICDMAAMNATNIQVSADIQRQMMLDQAHTDKQVQDQAGVHCDETCTQTFRRQSEVIDRTHEILKLARNLANKVSMNAHYDPNSGSSSRSPLMWAAFLGHGALVQYLRTTPTSIGALTDRFGYSAIGLAAMQGHLKVIKYLVDQGCQHDSPDVHGRSPFWYAARNSHYNVLIFLHGVGVYLDRKDNDGFTPLAVAARDGHADVVAFLLDDTRQRSHPHLDRWSKQPRDDFLSGTNSQQTPLFLAASGGHAKCVNLLLQLGSEALGDFNIQQAYAVAQGNSFQETCKVFESSRFPAALLHGHFSHYGNEFGYGMSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2N2</accession>
    <name>A0A9W8Y2N2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PTR2-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_009411</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2N2"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071916">
        <property type="term" value="F:dipeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000085">
        <property type="entry name" value="MFS peptide transporter Ptr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="401"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="479"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="520"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="546"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4363958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="608" mass="66778" checksum="8E0A6982FDE1E33B" modified="2023-11-08" version="1">MAATTNVKASLEITKGATAFDPIPLDTSSDSFEEDGTRLLGPFPNEEEWATLPRVAGAIPWQAWTVAAVEFVERFSYYGTSAVFVNFIQKSLPPGSKTGAGFLKKPGSGALGLGQRASTGLTMFNQFWSYITPLGGAWLADEYWGRYLTIQYSNIFAIVGHIILIMSAIPAVIVKPDVAIAIFSVGIVIMGLGTGGFKSNISPLIAEQYKDQKAYVRVKKNGSKEIVDPATTTARIYIYFYFLINCGSICGSLAMVYSEHFVGFWLSYTLPTICYLLCPAILIAMKKHYKLSPPTGSVMGKAFKLIGLGIKKSPRKNTFKDDGFWARIKPSEIRARGEVVPTWMTFDDAWVDEVKRGVLACKVFLWYPLYWLAYNQMTNNLVSQANTMNLGSTPNDIVSKLNPIFIVILIPIMDFIVYPALRKAGVKLSPIKKITAGFALSSFAMVSACVIQYYIYKMSACGNQINTLSKTRKDCNADISVWVQVFPYGLIGMSEIMASITKLEYAYTKAPNNMKSTIQAIALSTSAFSAALGQAFVSLSEDPLLVWNYGSVAVIAMFGGIGFYLTFRKADDEEDALNNLKESNYIGGREIGDAESAKYEKNEKGGIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2U9</accession>
    <name>A0A9W8Y2U9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2U9"/>
    <dbReference type="OrthoDB" id="3778180at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="470" mass="52338" checksum="250AB145E1304EE7" modified="2023-11-08" version="1">MSATSLPLAHVSVTTQARNHQESTMDANTHYAPLGASTSAPLTQTRLPTGDIQVSEMATNSARGSSADRDSREDVGATGSNWLQEDAPSYQRLGVGQGVRPSLAALQSSVYAGAPQGPALLIGGAHGIPARPQPLSSYHGTSLASRPPSWEPTRSQTLPIRPAREPTLFARRYVLELPSPGKIYYIPDGRHIPNSLIHKQKRQAGFFEHPALVVGTDRDIAYFYALTKAPPHAIGQLNMCLRIGTTTVDEGLGVLKLAAGSPPMLTETWVNLEQRFTIEWKNLDNWATDVRVDLDDTWKIWKRVQELEADQNRYLYKPLPRDMSVIQPGTVVMLPNNARSATLGAPVLVLENDFPRFYFLRIKTMSENMHFNPAAKRRNGNPRRLCLEISDRPKIGHDGTPVMLLEAGSPSMREQSYVEVRPRAKPDLLERAKSWCWPPVMLRPHSIQLLREFMRRAAARPAEEELEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2X0</accession>
    <name>A0A9W8Y2X0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008756</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2X0"/>
    <dbReference type="OrthoDB" id="10262413at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051783">
        <property type="entry name" value="NAD(P)-dependent_oxidoreduct"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48079:SF5">
        <property type="entry name" value="DEPENDENT EPIMERASE_DEHYDRATASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G00180)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48079">
        <property type="entry name" value="PROTEIN YEEZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="6"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="325" mass="34050" checksum="A64015F7604DC851" modified="2023-11-08" version="1">MSQQTIFITGASGYIGSLLTAHAISHGYTVRALSRTPSSDTKLSSLGAVPIRGDLTSHSVLTQEASTADMVLSLADSLAGNYGKITQEERFKVNNGAISALAKGLEGSGKALVVTSGSLYSAADPNGGETDETSPGRPGGFFGTDSHGKALGEGSERVALAAKEKGVRVCVVRLAPWVYGRGGSGVKLFMQGGVAAGEMIYVGDGAARTTTVHVEDAARLFLLVAEKGRAGEVYNATAETHVTFKQLAEAMGKAIGVPVRAQSYEDTEKKMGPFMTKFLSLENRASNRKAREELGWTVEAQKGILEEIADGSYGEVAEELRKAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2X4</accession>
    <name>A0A9W8Y2X4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2X4"/>
    <dbReference type="OrthoDB" id="4497018at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="355" mass="39985" checksum="EF161AE782847869" modified="2023-11-08" version="1">MFRTQATNSALEAIVMSSWLQRIRGGTGAEALPPSRKGTASSSAPKDQNVPKKRFQIHQKCLYERMLPGNAFVSAHVNRLQHGFYETSILHEPAIDTVFFVSVHFVFHPQDPRSHRFKSAEIEVCVHGDDDHPVAYDDPRYAPPTSHPRILKHAPELMFGAVSPENLQWNFSLSSSLGVSQAPVSATVNPTGGMKGSYKVYDMMSIQGSVRSKRSPFGPEFDLEDAMAVWTLEENLLQRSGLPREFDFVLLVQKPDDVKNVYLTVNVDAEVEAWYGAYPKWYTNMSKYLPTQDFHLDFNADIGQRFLPSEPGRGFNFASLPHPLDEYVTMPGTVYPTNVSCMFFATMLCTDLHRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y305</accession>
    <name>A0A9W8Y305_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SUZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008959</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y305"/>
    <dbReference type="OrthoDB" id="278430at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024771">
        <property type="entry name" value="SUZ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12752">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51673">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUZ" evidence="2">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="493" mass="53167" checksum="F8F81B71AC3E0502" modified="2023-11-08" version="1">MVDPAKSASTPPVELPARKIMRRDDGKSGTNTTANSQNPSKTTSEVGGSDGSNDGSDGKEKDKATLTREEREARYREARQRIFGSAESEENDATDGTGLGEEKERDNDVSRSSSTSGKKKVKKPRNYDDDDFQARSHFNAYYPQQYPGPGYTGDNVVYYNGFAAPMPNSQYPSMNPGASPSPPAYSIPYSGMMSPDAQPQYGWPSQQYQPSNSSMMYPTYGQAPNGYDLSTDFQRGMSFQNAAVPSQVTPKMTNPQMASYQDAYQQPQQMPMNSAWPQMGPQPSYPMAQGSYAQPGPGNRPMSAPHQAPMPGPYPYGQFPSSTYNGKPGRNQHPIPGSYNRQQFNPQTQAFIPGGRNMPYAMQPNMSQGQPHMNSFGGFQMPPQNILSAQIPRLSPPATNTPSFGSPQSIQGNNSGIAMRKTASQSRDEGIGKWGTPSHLPPKPPVPVPAPQSAPKFTLPSMTNMSSGLVNNAPAVSIRGGSGGNSLNGTNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3Q3</accession>
    <name>A0A9W8Y3Q3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Conserved oligomeric Golgi complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">COG5</name>
        <name evidence="7" type="ORF">N0V83_008347</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3Q3"/>
    <dbReference type="OrthoDB" id="18786at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019465">
        <property type="entry name" value="Cog5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048485">
        <property type="entry name" value="COG5_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049176">
        <property type="entry name" value="COG5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13228">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13228:SF3">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20649">
        <property type="entry name" value="COG5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10392">
        <property type="entry name" value="COG5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 5 N-terminal" evidence="5">
        <location>
            <begin position="13"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 5 helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4365727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="446" mass="48857" checksum="490E9AA5A7307870" modified="2023-11-08" version="1">MSKDEEPSYIDYEAFLGPDFSATSFANTLVLATNNPSDTPLDLSTPLSRVLFDVQEVDTHIDTLTTKSALPLLEHTRDSAESSGRILREVEGQVASLTESYKALEKEVIERYEVAEQVRVTAERLCETVKLGRAVARCLMLGRQLEVRMSELGGVGSAKKEDHRAMVRSADIILSLRQIFMASKPGEEGEGLDRVIAVTTLKNELVNPGERSISSRAQQVVKEFAMSSLLSSSGQTSASTYAQTEDTKSRTTSALLTLYLLSPTSAKTTLNTFEPTLMLNVLQEYLQIQLKSSLASLARALGQLPTLDRTLLEISARCQNIVALQSLLEGIKPPSHPLLDSSTTTGAQPWSNFLQPVLHALDTSSLPSYFWRSLASNLSPRVHELMQRGGVSARTLRSNKDRVRDMVRDSVNRGSQLPGALSTKTVAVTWEREAAVMVGSIMGQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3Y1</accession>
    <name>A0A9W8Y3Y1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3Y1"/>
    <dbReference type="OrthoDB" id="10516743at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="358" mass="40647" checksum="30F0CB3F1E850AAF" modified="2023-11-08" version="1">MPPKRRNAGKKPVIQRDPLVAECLDAIKQSRDAENTLAEIGDEDPEYEEQATTSAESQYQVVTTAYELAQRQALFKNEHVETDWETQMRLVKLLSLVQDARKAGPNTDCIADAMTKAAAELTKYETQHFGQKTRKSWAPSTKFDEKGQEKISGYELELSSDSSVGPSDADFAPYPPAEEVGQTWMVFGEGNDSEGVNMTLEKGSNIKINGGDDDDDAADEEDEEDEEDEEDEEDEEDEEDEEDEEDEEDEEDEEDEEDEEDEEDEEDEDDKHNKEHYKDDEIYEEDEEQIKRKSYQAEDEQAEQSAGETLNAGGESQHSSDNDADSFQYEDSFCHGCNKVKHGKRFEEAIALGLFFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4I9</accession>
    <name>A0A9W8Y4I9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007133</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4I9"/>
    <dbReference type="OrthoDB" id="4207724at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="362" mass="39105" checksum="33E6E463259291D1" modified="2023-11-08" version="1">METWMSWAVFLAIAGAAYWYYNRNSRPISRGRSSTRTSASNPLKDAVQWSDSESKPKAASKPTKPKAPRKSVKTAVQDIGNKAEAYLSTASSTAGADADDDLSPVASPTIAANKAPSGRDVSDMLGSQAAAPSVLSIKPSEKSNRPAKPQQQKSDVSQETKKQRQNRKKVEEAKAAREEEEKQRQALLEKQRRTAREARGEPAKNGLQGAKAPTSNPWTTVPSRGTVQGGPVQAPVSAPSSQLLDTFEGASSTASSNAPTNGTSSTQAYDSLPSEEEQFRLAMEDSAWTTVPKGGKKQRKTINDELLEENSSFGAVQDHVPAKPVRPTQAMKPENQKPSSRYQILSEPYTSKGDDDSDWPVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4K3</accession>
    <name>A0A9W8Y4K3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4K3"/>
    <dbReference type="OrthoDB" id="4828117at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="196" mass="20408" checksum="643F4B43C96B5123" modified="2023-11-08" version="1">MPVNFLDKETNDRLIAAIIASVDNKINPKEISRIYGDSVGYNTIEWYIRKIRHKAVGMKAQADAGSAGGVGGGAALPSAAASPPAKKKGAGTGTGRQKKNGNGNMQVAKSGGGGIEGMVTNVCKANMVSGVISGRVTKKTNGKKDQATTKVKSEVDVIDDDDLLFGTGFDDVEEDNASGGGIFDGRVEEVEVEEYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4S3</accession>
    <name>A0A9W8Y4S3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta-lactamase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_007254</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4S3"/>
    <dbReference type="OrthoDB" id="5946976at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050491">
        <property type="entry name" value="AmpC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46825:SF9">
        <property type="entry name" value="BETA-LACTAMASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46825">
        <property type="entry name" value="D-ALANYL-D-ALANINE-CARBOXYPEPTIDASE/ENDOPEPTIDASE AMPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040906226" description="Beta-lactamase-related domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-lactamase-related" evidence="3">
        <location>
            <begin position="76"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4367669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="412" mass="45814" checksum="A374FFEF01BAACAB" modified="2023-11-08" version="1">MMKFIVALVAAQRVLTTYATTIQAQHQEPLVEHAATSQEYNSTWKFRWHRGMLTGFPDNLAAEANFTGSMNSFMVNNNITQAQLSIAQNGKQLANYGFNWNDPSTPDVGPYDHFLLASVSKIYCVAALRELMKRHQVTPDTKVYERLGYSSGSAKDARVFNITVANLLDHLGGDDSWHLGSDPAYTMREIALAMSGGAHPATEREIIEWKLKRPLDFDPGNKAYCEEKYNQDFCYSNYGFILLSYLVANITGSPDYYTWLNANILKPQALDVTRWVTDPSAHAKDIVQQIDSGYGPSALNPLDQDPVPWIYGGDDMYKDSDMGGAALATSAGMLTKSLASCPAWGWQATRSPGYYRTGSTPGAFTYVTQRWDGLDVAMVFNTRKYNTDDVHGVLYAQIDGYLNSNRPSMVGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4X3</accession>
    <name>A0A9W8Y4X3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007763</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4X3"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843:SF7">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="62"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="267" mass="30178" checksum="FC0FB0B224A4D34F" modified="2023-11-08" version="1">MFSSSRTRYQEGSFSKHRASKSKSHKKNPSFRDSPRKVERLAQQAQVSSPQSNQTSPTLGSAIITISVGPEQRLFAAHEDVLSHSPWFQAACNGQFFQTANKRIDLPDEEPEVFSSILEYLYKGDYYPRMEYDKKRNSWNLENGGAGTDEAVVYSSAGPILKDTIIYCQAEKYGLQELKKLALKKQGLQSGIQCSTILTSARFAYANTPDNDSKLRAHYLALIIRARHTFKRSGTMQNEMENGGKLFFDLFVAMVNHMDDLSQKTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5D2</accession>
    <name>A0A9W8Y5D2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Paf1 complex component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LEO1</name>
        <name evidence="2" type="ORF">N0V83_006926</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5D2"/>
    <dbReference type="OrthoDB" id="20844at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990269">
        <property type="term" value="F:RNA polymerase II C-terminal domain phosphoserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007149">
        <property type="entry name" value="Leo1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23146">
        <property type="entry name" value="LEO1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23146:SF0">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN LEO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04004">
        <property type="entry name" value="Leo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="474"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="484"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="503"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="542"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="569" mass="62664" checksum="D148549AFDC3E182" modified="2023-11-08" version="1">MTAAEGVCRAVEGEEVGDTSRAASDSGPTSSLQGGIEKGRAANTHRESATSLLLGTLFTAASNQQFVLARASTFTIVSTFTGLFVLDRVFPPDTPLLLESNYLRIGRRSPPQPAMSDAALDSTLSDDEVVAEGTPAANGEEGTPRDTVEEDGLDEEVDDLFGDDDDAPAPQRKLDDEELDSGDDEGRADRARAADVAEEGEPQTFAFMDADLARHAIPEPTDNELYLLKVPRFLSFEPTAFNHKTFQPPATDHHSKLAASEQFSAFNTAMSTIRWRRSPSNNAELQSNARILRWSDGSLTLQFANDPTTQFDIDANTLAPPQINPKIPTPTAIREDKTGSKTSAKKESYTYLVAPYEEANVMRVTNKLTTALSVVPAANLKDAALEKLQNDLAELATRGRDESDNAISFVDVNEDPELHRQREEATFKEKQRQARAREKHEARQAERANRTMGRTGHRAVGGLDLDELEGAPRGPRKPRQKGGLRRDWSDDEDYGRVGRSREDDYDEEDDFIAASDEEPEIVEDDDDPDEGILASPKRGGLGDEEDDDDEVVISRTKRRRVVDDDEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5I2</accession>
    <name>A0A9W8Y5I2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006806</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5I2"/>
    <dbReference type="OrthoDB" id="3358750at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39475">
        <property type="entry name" value="CONIDIATION-SPECIFIC PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39475:SF1">
        <property type="entry name" value="CONIDIATION-SPECIFIC PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="120" mass="13693" checksum="B371861C891840BA" modified="2023-11-08" version="1">MSGQSNVGNSQVYEAGDQRTVPDAEIEQAKKEQRFHEGKEHSHKANDSISEDERTIANKLAREEKREQEDDEPKTEEDRLRKEDATLPAKAHGNKPSRGAVIDQQLRDEEEEELKRKGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5M9</accession>
    <name>A0A9W8Y5M9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006748</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5M9"/>
    <dbReference type="OrthoDB" id="3794829at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="222" mass="24567" checksum="DACC35FFE6A95B7F" modified="2023-11-08" version="1">MCRYSKKRHTCGHESDWLYIEMCHPGFMANAVCPDISGDSTPRSSHFPCYNCIKAEARAEIETRALLEQEAVAKAIEAKEKAIKEKQAADFKAKEERIRREAKEKAIREREEELRVKREKEEQEQRAKKEGGLWIETANLKNRKGRKGGANGGPPTPLSAPAIMKTVTDKKENSPKKDRNIDTGGGRVGHWGPKKILSRKENANVGATGGGGSNGNANAEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5Y1</accession>
    <name>A0A9W8Y5Y1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase</fullName>
            <ecNumber evidence="3 14">2.4.1.258</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Dol-P-Man-dependent alpha(1-3)-mannosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ALG3_2</name>
        <name evidence="15" type="ORF">N0V83_006400</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 14">Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the first dolichyl beta-D-mannosyl phosphate derived mannose in an alpha-1,3 linkage to Man(5)GlcNAc(2)-PP-dolichol to produce Man(6)GlcNAc(2)-PP-dolichol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29527"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="Rhea" id="RHEA-COMP:19516"/>
            <dbReference type="Rhea" id="RHEA-COMP:19517"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:132515"/>
            <dbReference type="ChEBI" id="CHEBI:132516"/>
            <dbReference type="EC" id="2.4.1.258"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12 14">
            <dbReference type="Rhea" id="RHEA:29528"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the glycosyltransferase ALG3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.258" evidence="3 14"/>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5Y1"/>
    <dbReference type="OrthoDB" id="20028at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052925">
        <property type="term" value="F:dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007873">
        <property type="entry name" value="Glycosyltransferase_ALG3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646:SF0">
        <property type="entry name" value="DOL-P-MAN:MAN(5)GLCNAC(2)-PP-DOL ALPHA-1,3-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646">
        <property type="entry name" value="NOT56 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05208">
        <property type="entry name" value="ALG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 14 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="340"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU364047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4368045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="421" mass="48450" checksum="D8BF34D670E03CAF" modified="2023-11-08" version="1">MSQLIRQALDLAYNPEHTRWMIPLLLVADAALCGVVIDKIPYTEIDWTTYMQQISIYLKGERDYAKISGSTGPLVYPGAHVWIYKYLYKITDEGRNIQLAQYIFALVYLVTLAVVMACYRRAKVPPYVFPLLVMSKRLHSIFMLRCFNDCFAVLGLWLAIYCYQRNQWHLGSVFYATGLNVKMSLLLPLPAMGILMVQKLGGREALTQAMIIMQVSVLYGYPFRKRAPSYFSRAFELSRVFLYKWTVNWRFVPESTFLSRPFALSLLAVHAGLLLWYARTRWIKPSKRDFRGFLKLAIPAAEPRDQDDMARRITPDFIMTTILSATTIGMLCARSLHYQFFAYIAWTTPFLLWKAGFHPIVQYILWGAQEWAWNVYPSTPASSATVVGVLAITAAGSWWGTRNEYEGAMKGIIEDDHEHEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5Z3</accession>
    <name>A0A9W8Y5Z3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">6-phosphogluconate dehydrogenase NADP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_006936</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HIBADH-related family. NP60 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5Z3"/>
    <dbReference type="OrthoDB" id="435038at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015815">
        <property type="entry name" value="HIBADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051265">
        <property type="entry name" value="HIBADH-related_NP60_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43580:SF8">
        <property type="entry name" value="6-PHOSPHOGLUCONATE DEHYDROGENASE NADP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43580">
        <property type="entry name" value="OXIDOREDUCTASE GLYR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000103">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase NADP-binding" evidence="4">
        <location>
            <begin position="9"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000103-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4367355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="330" mass="34969" checksum="55372992AD0691EF" modified="2023-11-08" version="1">MESTEGLRLGWIGLGSMGLAMSVNIQKHLKRNALPPMRYWNRTLSRGDVLKEEGATPGHSIPEVVQTCDVIFISLESLMPCQVSDDEVLKGVVDEVIKSGSIKSKIFVDTTTVHPSTTTSITTLLGNEEASYVAAPVFGATPVAQSGSLLMAVAGPSQAIETISPFLKGVIARAVIVVGSEPSQALLLKTTSNFITAGLMYLLSEAHTFASKTGLPGEVLESLIEQNLGTYAHGVSKRLTSGAYFPAEGSAPSSGLELGIKDVGHGVKLAREKGMKLDVGELYLEAAEEAKAFGDEKGRKCDSSAVFGIVRQKAGLDFENEKVKERDAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7A5</accession>
    <name>A0A9W8Y7A5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TRIP4/RQT4 C2HC5-type zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006078</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7A5"/>
    <dbReference type="OrthoDB" id="338816at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180022">
        <property type="term" value="C:RQC-trigger complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039128">
        <property type="entry name" value="TRIP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009349">
        <property type="entry name" value="TRIP4/RQT4_C2HC5_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963">
        <property type="entry name" value="THYROID RECEPTOR INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06221">
        <property type="entry name" value="zf-C2HC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRIP4/RQT4 C2HC5-type zinc finger" evidence="2">
        <location>
            <begin position="239"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="487"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="540" mass="58742" checksum="EF33BA0EA8851F49" modified="2023-11-08" version="1">MDVSAWAVPQLSRLLPLDDESLKQIISYTESLPNREAADHLQNLLGDSPQALEFITSFNSRRKPKSGAASSQTQPSNDSSEVPKARPRKKKQQNLGKLPPQRQPENYGNVSGGYIKKDEEDYMAGSSRSRAAKSHMFGLTETPDAVQKPTIVPLSGSATPKSRGVSPSPPVSKLPPSAAGQLISETKSRNSSPKPKAKVSVAGGTPMHGASTALNDLESAIRALEIQTNPSLASQDNAKRKCNCMATRHPLLEAAPNCLNCGKIICVKEGIGPCTFCNTPLLGSQEIQSMVRVLREERGKEKMAANNASQKRADVSLAPRPFSTPRATTPASSNPTSDVDSEPEKLAKAKQHRDKLLAFQAQNAKRTQVHDEAADFETTTAGLSMWASPQERAMQLKRQQKALREQEWNARPEYEKRKVVASIDLSKGKLVRRMAAAQRPATPESEEDEPIEDSAQGPTTYDHGAISSGGGAFSKNPLLGGLIRPTIKVEESKGKEKDGERKQTWRRVQDDNDDNEQWILDGGVYGARAENVGLEMDGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7W0</accession>
    <name>A0A9W8Y7W0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_005413</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7W0"/>
    <dbReference type="OrthoDB" id="5282002at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="5">
        <location>
            <begin position="172"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4369651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="221" mass="25182" checksum="9845731A5F7C57C6" modified="2023-11-08" version="1">MPNPNWPAVIPIPEETITFMSPDTTKTTRTDFTDFFQRFRPAEDAHPLYRHLFLTHQELAKALIEHPAMRPNLEQTFSTPANSKNKVYFMWDFVLRTFQILVAQVNPQNPYRSPTLGDIVGRATMARGLTLDTEGQLEAMNASVGYSDDAGVDFGEEIKRLAARLDELPEVCAACKKQREDGKPLLICARCKDEKYCSAECQKKRWKSHKKECKEGGIDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7Z2</accession>
    <name>A0A9W8Y7Z2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_005436</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7Z2"/>
    <dbReference type="OrthoDB" id="3711359at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4369674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="351" mass="39514" checksum="15A28A0FBD1ED341" modified="2023-11-08" version="1">MADGILDQPRLAPGTCATKSLSFFERLPYDVRAIVYTYLEPGQPPPIAPGFQQTSSGFILSCQRAKEEIEEIPRMNVANLLREFKATFERNTSLTIHIPAISRSSTINQLRNVTVILPFTSLHFLRSSTCDYMWKREVLAGLHPLFALYFSKIRIHFFGPENQPACATLLDRGKVEVSMHSLLRDVTYMLERVNSKKTSGTNVTVEQIFRFDAKRRSEAYPSARVNTKQICLSWDLMEGEVQGKGDGAALNGKLHRNPEPGVHVDGGKAGAESFYHLRDKERLVGEMGIVSNSRWALADDPRLNSLLNAIETRWTYCSSGGLGEHLREGLDGVSETEFEEREQQVSRLIYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y815</accession>
    <name>A0A9W8Y815_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_004875</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the DapA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y815"/>
    <dbReference type="OrthoDB" id="191315at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00408">
        <property type="entry name" value="DHDPS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128:SF66">
        <property type="entry name" value="4-HYDROXY-2-OXOGLUTARATE ALDOLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="DIHYDRODIPICOLINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="3">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR001365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4370357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="327" mass="35359" checksum="6FE987E65D0CA476" modified="2023-11-08" version="1">MPSNETYANGSSNGTSAHSFPPGIHVPSLTWFKNDSDQEIDWDVQDEHFEFLISNGLHGIVIAGTNGEAATLSSAEKTQLVQKARKAAQRLNRGDLSITVGGVAGCTRDAIQQTIDAKAAGANAYLALVPSYFHFAMDQDAIVAFFQELADGSPIPIVIYNFPNVVAGLDINSEMLDILGKHKNIVAVKLTCGGIAKVSRISASFRKSEFVALAGQSDWLIPALSVGAVGTITGVANLYPKTCIHLFNLYQQGKYDEASALQIKLSVTEWGFGKGGINGTKYVVSHKRGYPESSSDCRRPYPRYTDEEKRKWVVNQVSGLDEIEASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8L0</accession>
    <name>A0A9W8Y8L0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8L0"/>
    <dbReference type="OrthoDB" id="3799222at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="158"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="268"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="164"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="314" mass="35637" checksum="9E42E65B6F54DCCD" modified="2023-11-08" version="1">MNSCTDGTGEILEKLHEEWDHIPFYNDVCDAHDQEEYRGDMLAYMVYIQHFTTDGLNFAYYIRGGANIAKFCAGAFGMQVYSGPIFGTPVVLNGRKIMLSGHADLKDQHDFTRVRKHGPQTEKVKWRYMLETARLLWNTMEFQMDIYAELGHKRNLGADSGMDNNRDRAAGRDPGADWEVESVHSNETSQSEKMRGKMKMKEPEIEGPEIKSEQAEGTAIRDNLIAIKREGGSADDLLDYLREEDLGSRTSSGPSVFTVKQESLITSLHDARNLLGDLEERRVDFEARGVPMSEEIKELESALKRDIEGLLEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8N5</accession>
    <name>A0A9W8Y8N5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8N5"/>
    <dbReference type="OrthoDB" id="3787000at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="401" mass="46374" checksum="317BB07C908FD520" modified="2023-11-08" version="1">MPITTTIIDPNGDLVLVLKNPIYSFQPFQKVAKPRAPPQNAVADLYLFGMPLTKSIDSLEDEFNSLSLAADTVGVEAKDQNDKDEDDRSDQDEDDQSGEQEDIEDAEFHFLVSSRQLRIVSRYFDVMFKREFLESIIQEDGKYRVETQDFHPKALEHVLNVVHVQNRRVPKKLSLRMLAHICVVVDYYDLKDAFLPWASLWTDSLKLYDIHVGRDVVYGERHIWLVFVGLVFEQEREFKMATNSIVCTCPDFTQDLEFPRVDSVLNKLFNWRLLSVEIMIHRINELKEKVQNGELGCSNQECQTRALAYVEKQLKRAETLLPKEGSSFMNIHFRMEVAGVEFLRSNYCSCLLQHANQPCCSSCRNPDPLSFEHIIEQARTPLGFFRPQKMGFPEEEGFEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9D7</accession>
    <name>A0A9W8Y9D7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_006107</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9D7"/>
    <dbReference type="OrthoDB" id="272271at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050411">
        <property type="entry name" value="AlphaKG_dependent_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696:SF54">
        <property type="entry name" value="FAMILY OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G13850)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696">
        <property type="entry name" value="GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="3">
        <location>
            <begin position="76"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4369025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="390" mass="43386" checksum="0F3874C1B72146B0" modified="2023-11-08" version="1">MSTHTLSPKSSNESLQSTSSTFSFPDKLSPAVTGPMVWKGDELNPAKYVVELSKDEIRDVRAAVIKVKIAGTPRSEINQETFDLGNPELAHKLSLISKEIHQGRGVAVLRGLDAANFNDEEAVIAFAGVCSYICLERATDSYANQTLSHVRDATKDVVPRWAKDIGLAGSKITAAMDFHSDRFSGDILALHVRNDGGKDAGGEQFIVSFWTIYNELLEKDPEVLETMAEANWPFELKQKDRAPYLELGPTLFFSNGKPICQLVKAPLLGTPLIHRDPTMPVVTKKQMHAMHAVEELAKRFCTKLDRQQGDIQFVNNLSIMHARGAYRGNNGKPSTRHLLRMFLRDPANAWGKPVRFRNNFDDPFAVGRPQNLPVLDSDPWRLISGRESHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9J9</accession>
    <name>A0A9W8Y9J9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004335</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9J9"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="173" mass="20142" checksum="59B9D7DF9E15C13A" modified="2023-11-08" version="1">MDPEDDIEEQSRKEEMQRREDRRLRDEKVDEICRTGSAADIRAETDKLKDILKEKEALLMKLKAENEQKCQDLLFGDKRAEIDRMQKTLVSLGEVPEHAEELIDRAIADLQTMKAKLRERTKDLRETSELFRDLGTHLNEHLKDIPGFLPFYTGKSKAFEEAENLARASKGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9T3</accession>
    <name>A0A9W8Y9T3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7703 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_004962</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9T3"/>
    <dbReference type="OrthoDB" id="405906at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056120">
        <property type="entry name" value="DUF7703"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37013:SF3">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_1G05950)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37013">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_1G05950)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24802">
        <property type="entry name" value="DUF7703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7703" evidence="3">
        <location>
            <begin position="46"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="330"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4370444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="422" mass="46793" checksum="84CD0CE613B571B1" modified="2023-11-08" version="1">MASSSMSAVASATSSALPRDTPAQGSFESAGAGITGGYTGDSLSLQIIIAFLIGLSLYNALELIVLAFVTFQRYRGLYFWSLVVSAFGIIPYSLGFLIKFFQLLDPNQKVGYVAVFFLSIGWYPMVTGQSIVLWSRLHLVTNSRRVLRWTMYMIVTNAIILHSTTTVLTFGSNSNSLSRTTLAHFVRGYSIMEKIQMVGFFVQEVILSIIYIRETVRLLKLSESVQDDVRSFDDGTGTGNLRNANVRKTMYQLLVINVIIITMDLALLGVEFANLYLIETTLKGVVYSIKLKLEFAVLGKLVQLVRVRASSNNNSDPHRHSSQERRATSTGLTLEKTNTGGTGGGNGRRVISSPLGHDFGNSNFPDFVDPRRVSADITHAEAANGDDGWETRTGEESEKWRRRTRVNRGSWIEEEMDKHNIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB17</accession>
    <name>A0A9W8YB17_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB17"/>
    <dbReference type="OrthoDB" id="3793866at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38790:SF4">
        <property type="entry name" value="2EXR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38790">
        <property type="entry name" value="2EXR DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="284" mass="31918" checksum="5A93655A77A592ED" modified="2023-11-08" version="1">MSTCESHSNLELCIFNVVSPLPSTITRQPANSTSMSSLGQRVRARFKNPPYSIITKDAQSHLSHPKSPTDKRHKTLEETATPPDPRKSPAELAAITQQNQQTSPFLRLPGEIRNTIYHYALAGHEIYAMPSGLTHRPFTCQGRPTSQFAWSLLPISQLLALHLPLVCRQIRAETGKYFVFKFNEWGSTMPEYFELLMGVLTQEQRGRIEVVRINWAGMERGAGRRVVAMVDGSEYAALGGLRNLKVVVLRDFGGMDGQMREKVVRVFRELAGKKRLEVRVESIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBG1</accession>
    <name>A0A9W8YBG1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004213</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBG1"/>
    <dbReference type="OrthoDB" id="15189at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05471">
        <property type="entry name" value="pepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034164">
        <property type="entry name" value="Pepsin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF47">
        <property type="entry name" value="ENDOPEPTIDASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01220)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A1" evidence="2">
        <location>
            <begin position="43"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="286" mass="30601" checksum="E1553E4ACDDDF995" modified="2023-11-08" version="1">MRLVALLPVLTSASLDSRTVLSLNRRQQSKIKPITLTKFGVTFEAEVQVNNRTFFLISDTDSSDLWVPVADFQCVNSTSGQDVSQEECQFGGTYQVPNSTEYVANQTLGVQYGTGLALGKVAYADVTLNGKVGLVDRTDDLGDGIGSGVLGLGFPALTSAHPGTELDNNTLPVNRVPYDPVSVSMYKQGLVESWYSFAIERPPKNATSGPGGWLGLGEMPPVAHPDDWAIKPIEVTDGLPDELTGWKRQITPMTLSVDGVTWGNPSSNSSVTNSTTFQAVVDTGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBJ8</accession>
    <name>A0A9W8YBJ8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CPR6</name>
        <name evidence="14" type="ORF">N0V83_003258</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family. PPIase D subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBJ8"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000029">
        <property type="entry name" value="peptidyl-prolyl cis-trans isomerase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000009">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0697">Rotamase</keyword>
    <keyword evidence="8 11" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="13">
        <location>
            <begin position="10"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="309"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4372967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="374" mass="41348" checksum="0B6FC5678D26BD34" modified="2023-11-08" version="1">MADSKRSRVFFDIAIGGTKAGRVAFELYNNIVPKTAENFRALCTGEKGVGSAGKPLHYKGSSFHRVIKGFMIQGGDFTQGNGTGGESIYGEKFEDENFERVHSKPFLLSMANAGPGTNGSQFFVTTVPTPHLDKKHVVFGEVINGKSIVRQIENLKTQSGDKPFQDATIIDCGELSGEEYDKAADKVSDATGDPYEDFPEDQKSGEEEWEGTQILKIATELKDMGNAAFKKGDLDLGIKKYQKSIRYLHEYPAPLETDPKDLWPQLNTLKISVYSNSALLQNKTSQFAEAVESATKALEIEGITEKEKAKAYFRRGQAKIGKKNDEDALADLNEAAKYAPTDAAITRELEGVKKRVQARKEKEKKAYKNAFNFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBS7</accession>
    <name>A0A9W8YBS7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_004356</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBS7"/>
    <dbReference type="OrthoDB" id="3360544at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018506">
        <property type="term" value="F:maleylacetate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08177">
        <property type="entry name" value="MAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056798">
        <property type="entry name" value="ADH_Fe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034786">
        <property type="entry name" value="MAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496:SF105">
        <property type="entry name" value="REDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G07090)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25137">
        <property type="entry name" value="ADH_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA" evidence="4">
        <location>
            <begin position="10"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Fe-containing alcohol dehydrogenase-like C-terminal" evidence="6">
        <location>
            <begin position="166"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Shikimate dehydrogenase substrate binding N-terminal" evidence="5">
        <location>
            <begin position="376"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="543"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="555"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4371140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="619" mass="65872" checksum="5FE852A31022F0BC" modified="2023-11-08" version="1">MDAFEYNGNPARVIFGSGTINRLPEELSRLNLTKPLILSTPQQVSQAEDVKKVLKGQVVGLFSNATMHTPTEVTEEACKLATSTQADAVVSIGGGSTIGLGKAISIRSGLYHIAIPTTYAGSEVTPILGETANGRKETRSDPAILPGTVIYDVDLTLTLPVSLTSTSGVNAIAHAVEALYARSGNPIISLLAQEGVRALASALPDLIADPTSKDARSKALYGAWLCGTCLGSVGMSLHHKLCHTLGGSFNLPHAETHTIVLPHALAFNAPKIPQAMKMLADALPEGNGDAVRGLNVLLSKLKVERALKGLGMKEGDIDKAADIAVSKPYWNPRPFERNEIREVIRRAWAGEEARVDNMATTATTTSSSEDKKTLHLVGLGVQHSIAPPMHNHITSSLHLPWTFHATECATIDSLLSLARAPTTAGLVVTMPYKNTIIPHLDALDPLAVQIGAVNNVYRDPTDPSLLRGTNTDWLGIKGCLLEKSSSAQGEPVVVNKPALIVGAGGASRAAVYALSAHFHASSIYVLNRDDAEVADLIRDAQRLSTSPPQPAGPQHHARQNPRPARAPRHAALHRRHSARLRAPIPCRESRAGVSRGVPSERGKGRRAGYVLQAKAHEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBW8</accession>
    <name>A0A9W8YBW8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">NEDD8-activating enzyme E1 catalytic subunit</fullName>
            <ecNumber evidence="8 10">6.2.1.64</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">uba3</name>
        <name evidence="12" type="ORF">N0V83_003952</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalytic subunit of the dimeric E1 enzyme, which activates NEDD8.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.64"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.64" evidence="8 10"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBW8"/>
    <dbReference type="OrthoDB" id="10255449at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019781">
        <property type="term" value="F:NEDD8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01488">
        <property type="entry name" value="Uba3_RUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.520:FF:000001">
        <property type="entry name" value="NEDD8-activating enzyme E1 catalytic subunit"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1913"/>
            <end position="1922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1973"/>
            <end position="1984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2023"/>
            <end position="2033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2040"/>
            <end position="2061"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="2127" mass="231498" checksum="0D8C11EA48DA3356" modified="2023-11-08" version="1">MIVSRSSTAMTSTPEKSITRASTATDSPNDEKRKRRFSLSSPFYRSNRSRSRPSSIALPSNTPSIFGNTPRNTPPRELAGGFSENSRPHSYHALDSWNFAPGVQPSFDQTQTTPPRSTPQSQSRLGILPSPAKSAFSSQEKDIEQDIPPVPPLPDAIQGGQGRGVSQDDASHRMLQSVIRYATPPIPTTEAEKPVQSPGFTEAGARGPRVLDTGKALPPAPAEIDDDDDLPPQLNHDPLPLATKPPPGEISSAIFFAGPSQVDVSDEDDDGKRSQVDASSRVRAPSSQPVAGDVSPLMPPVQSSLDEISDEDDGLQRRTGGTGGSEDDSHRQERDQDAGRFVKTALNVLDKYHSPKTETLESHHAALTSERKADEPRLATEDRRRATDASPVNMINQNHVTGTGLTAKRPSFETELIGDDISPVSMHSRAREIADDYAPPKVTKPVSGSAKSDLYVPKARAKDTLDSQEGSPRIGPRLHLPEQDDAVSKLSDQNHSSGQTGEIGLAVPGATGAATRDGSLSPGHASPPHLHANAGADPVDHPTRRVSATPFQVVHAVEEYAASNSSLASWDRDSIAARSHSVVSHPDEMRDESDLVTPVAQVPRITQNGPQADKARAPYDDTSNMATQNGYFRGHVASAGSMHQNQQLQPPTSDMTVPERSKSLLSQISAMVSEGGNPISPASSIAGRSTPSTIRRMQHDSSTSTKAPWTTAQIPEESTAAWNDQTPTAKDDGFDLYADHNGIVKDVRDESGQPLRVADAQVPGAPQQASLPNPTGPATVAAVDSKNEDRPRYSTERPMSFISGPPDQDGKPQDQINQFALQNDTHVTPAPEQFRQQLQQSDTPPIGTAYSVAPPAAVIQHQLPAQSQQRYPSEQQALQGFVFSNNDLQNGSANVQSNERTRAPVTSPSLPPPQDATQPLTSTERPTVDGQVPPNNQLPTRGPLDPRMYETAPGQPMLQGSPAHGQMLMPDHDPRAQGQPLGPPSGPRNQYEYQQQMMQLQARYARSQGAQNHTPPVATQQSSSQPPKQHAKPSSKPRLSSVFKNFGGKSHNIQQSSTMPTNSNLKPLPADPNRNASFHSAVSSLSLSHETTSMGRPGEQPGSFVLSNRPPSMGAESRFSQVSQGSTRVQPTDSRVDLRKPASPAPFYGIPPQQLTNVPPIPNGQPHQQHAQQQPQPQPQPQSYHTATPGNQEAGKKKRFSTLGGLFNRTGLAGDALPTKLKISKDEKKALKAQRNSTAPLAQAPVSQWPPQQQQFRPQQPGMAYPPGQVPPQMSSGMRPMGQQFVPPQTMSSVPQGMQGSQGVQDISSQRLPQQHQQFQAQSPGSVRPNEGSAYFRTKQLADEHQAQRIPAQSSGQGSRPNAEATSTSFDHSPPNLRQTSMGPPAGGYYKPDTKQTTADQGAYAASQAAQQQALERAHQERLREKAAYAASPLEHSQLQQRRQPMQGQGVSAVSQAERLQAQQRQRPMSDQSAYASSQAARAQGPQQRPQPPHEQSAYGDSQAKRQNAPPQREQQMSEQMAYQNLLAQRQLAERQRQQPVSGSGGHWPSQDDRVLPQQQQQQQQRPEQQFHPQYRQSYGQPPSTNQRSFSGPVPGHLPHAQSPASQRHVSSPLGEPQYEAPPIPAAYNHVSGAFVSPRDRQQQPLFPPYNPPLARPSMDQYGRQNSDSGMPSISPQVSAQSIPPNSRTHSDASTVSVISPISNTPDLPTAQPYANQRPQKPRMSSISEVHLQEQPWHLNFPQGATEQEIVRARQRQYMEQQFTAQQQLHAERAAQSPSTRSSPHEQSQTPEGQRPGGFKELLPRNSPQPYPMSQPVQSSQQEPPRASDSPQPLQPAPIHPDQPPQPAAYPLPMSPDNVQSPVNPLAGALPPPQLPPKLPHSPMRPMFPPTIQPPSEVQQHQQQQVGREPQRYETSQPPQSNDPQDEQPMPDEPPPSYNGPPSYDGPGVSNDGMDKSRPEQPRPPNITTDALPGERGRQQEPRQRQPSLGMLQHPQPASMAASPQRSSADMGAESLRRQLLQQEEHLRMERIQRAQIQRAETERERQEREAARARARELERSASGGGRVGSLRSVAGSRNGGTPGWERRGSSSRPVFELPAVEDDEPAMKATSYPGQEWVPPMWTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCX6</accession>
    <name>A0A9W8YCX6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_002567</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCX6"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000004">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF300">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="120"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4373828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="321" mass="36857" checksum="81A6A17145ACC00A" modified="2023-11-08" version="1">MAENEVDLDSIIDRLLEVRGSRPGKQVQLLETEIRYLCTKAREIFISQPILLELEAPIKICGDIHGQYYDLLRLFEYGGFPPEANYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFVLRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIIDEKIFTMHGGLSPDLNSMEQIRRVMRPTDIPDCGLLCDLLWSDPDKDITGWSENDRGVSFTFGPDVVSRFLQKHDMDLICRAHQVVEDGYEFFSKRQLVTLFSAPNYCGEFDNAGAMMSVDESLLCSFQILKPAEKKQKYVPSLGGGRPASPRTKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD36</accession>
    <name>A0A9W8YD36_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Pyrimidine nucleotide transporter, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">RIM2</name>
        <name evidence="12" type="ORF">N0V83_002620</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD36"/>
    <dbReference type="OrthoDB" id="269120at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015218">
        <property type="term" value="F:pyrimidine nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990519">
        <property type="term" value="P:pyrimidine nucleotide import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049562">
        <property type="entry name" value="SLC25A33/36-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829">
        <property type="entry name" value="MITOCHONDRIAL CARRIER PROTEIN RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829:SF4">
        <property type="entry name" value="MITOCHONDRIAL CARRIER PROTEIN RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="51"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="172"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="285"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4373881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="381" mass="41710" checksum="0729C0C6C00581C8" modified="2023-11-08" version="1">MTFEHASEPRIRDNNTQPLQTLPPSSRELGEVTPPKQPDGAPVATENISFAKSWAHFVAGGLGGMASATLTAPLDVLKTRLQSTYYQQHLAAMRSARGLPPIETMSFARSSILHIRETGEILWQVPKAEGWRALFKGLGPNLIGVVPARAINFYAYGNGKRLISSHFNNGQEAAWVHLCSAAAAGIVTGTATNPIWLVKTRLQLDKNTHADGRGRQYKNAFDCTMQTIRKEGVKGLYRGLTASYLGVTESTLQWMLYEQMKLSLARREARVAASGQPPTVWDQTVALTGKVSAAGAAKFVAALITYPHEVVRTRLRQAPQQDGRLKYTGLVQCFRLIWKEEGMAALYGGLVPHMFRVVPSAAIMFGTYEGVLKLLGESSNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDG0</accession>
    <name>A0A9W8YDG0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_002074</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDG0"/>
    <dbReference type="OrthoDB" id="411372at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045072">
        <property type="entry name" value="MKRN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11224:SF10">
        <property type="entry name" value="IP09428P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11224">
        <property type="entry name" value="MAKORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="248"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="337"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="368"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="429"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="479"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="512"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="530"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4374995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="583" mass="61675" checksum="F3077D20216887A3" modified="2023-11-08" version="1">MAGANGNGPMAVPGGRMNGHGNGANLREMGFGGPRSPPNNKNTSHVPCKFYRQGACQAGKACPFLHSDEPLTERAPCKYFTKGNCKFGQKCALAHILPNGHVVNRGNVGGHFGRMNIPQHQEPPMTNSLLTMQAHLGGMQPGYPYPMQDDYASQKNQYDMIPTIDTTFSSHPGSNYGSPPNENGRLPMSPVQKGLSVMDVPLPASFDSQGISHMARYGPIASSVPATFLANSPPSSYHADSPALRNLHDSAFGDGSRSRAAALGSSPPESVEQPVGRRMLHSELAANRSRGMMSSSVGARPTYKDEEWDETDMIGKFEEDLLPNSLSDLLTPQEQMRRFSRDQGDNSTPLSRLGASPTASDVKVGSPSAATSSPSRFQSLWGKSRGPTYESGFESGSLPGASAFGHVGSPLRNSTLHPGASPSLRAMNRPTSGDTSPFVSSPPRQASMSMISQQLQRTRLSSREEGGLTSNPMHPGINRVTSGSSIGSPSGRLGMDRAVSSSSINRERIEEEQGLFSMEEEEDLNKSKDGASTTSSTSNKRLSGLSWGSGGGKGSPNLAPVGSQRATAISGLGKETGPWGANT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDH7</accession>
    <name>A0A9W8YDH7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tyrosine specific protein phosphatases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001822</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDH7"/>
    <dbReference type="OrthoDB" id="449382at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026893">
        <property type="entry name" value="Tyr/Ser_Pase_IphP-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13350">
        <property type="entry name" value="Y_phosphatase3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="2">
        <location>
            <begin position="80"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="280" mass="30490" checksum="80E52C41DC033AC8" modified="2023-11-08" version="1">MEAAGVKRVFVPVFEESDYSPERLAERYVKYMDEDVRGFVAAYEDILGSAGPAFGRILRYLAGLSPPPSPSPSPPSLGSKEQEETVQRDEGEGKKTGKKEEPQAALIHCTAGKDRTGIFFGLLFDFLAVPRAQIAAEYNLTELGLASVREDVVARLMQAPAFRNYMLSRMSKNTTTTTGTTTGTTTTDTEDIANSLRAGQQQQEDASQSAAEAVEIPPALLEKGRQAALRMVSARKESMLGALAMVDEKFGGAEQYMRVQCGLGDGELEALRRNLVVRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE62</accession>
    <name>A0A9W8YE62_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_003231</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE62"/>
    <dbReference type="OrthoDB" id="3626597at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06228">
        <property type="entry name" value="M14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000155">
        <property type="entry name" value="Zn-dependent exopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF143">
        <property type="entry name" value="SLL0236 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040946498" description="Peptidase M14 domain-containing protein" evidence="8">
        <location>
            <begin position="17"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="9">
        <location>
            <begin position="129"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4372940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="488" mass="55094" checksum="9C61F18B3FA1F9A0" modified="2023-11-08" version="1">MKVYNILALLPSTVLASNVLSVAFFAKEKSQLYDFISTNYELDYGCRPVVMAVGNEHKLHAIVPEEQLYRLKRSLDPDIVRVETIHNLNKRQTNTAPIGKGDRFKGGTIAPRGLGTRKATEYSEFQSAGILNADEVYAAMKGLEKEYGLTLFTPPHKTFEGNEIIGGVANKAEKIKKDKQYIYFTSGIHARERGGPDNLIYFISDLLYANKNRIGLRYGNKTYSNKEVKQALGAGIVFIPMTNPDGVRHDQANSNLWRKNRNPSSARPNEPLSVGVDLNRNFDFLWNFTKHFDSSVSPASTNASSQAFYGTAPFSEPETQNMAWIYDEYPNIRWFIDVHSAAGTLLYSWGDDIDQSHDPKQNLFNPDYDGKRGIVEDNIYKEYIDQEDWDNIAFQANRTTAVMISVGGRDYVPQQAVGLYPTSGASDDYAFSRWHQSKKVNKVYGYTMEFGYPTNFYPTSAEFIQNILDTNAGFMEFILTADEIGLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEG6</accession>
    <name>A0A9W8YEG6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="9">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_003668</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Esterase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the carbohydrate esterase 1 (CE1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEG6"/>
    <dbReference type="OrthoDB" id="2425929at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010126">
        <property type="entry name" value="Esterase_phb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050955">
        <property type="entry name" value="Plant_Biomass_Hydrol_Est"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01840">
        <property type="entry name" value="esterase_phb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43037:SF3">
        <property type="entry name" value="FERULOYL ESTERASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43037">
        <property type="entry name" value="UNNAMED PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10503">
        <property type="entry name" value="Esterase_PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0964">Secreted</keyword>
    <keyword evidence="2 9" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041014630" description="Carboxylic ester hydrolase" evidence="9">
        <location>
            <begin position="21"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4373373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="292" mass="30913" checksum="6FE50F0E42461295" modified="2023-11-08" version="1">MYIPTAILTAIATIAATANGALTRVNDFGTNPTNLQMNIYVPAKLATNPAIIIALHYCGGTGEAYSSGTKYNSLSDTKGFITIFPSTKKDNNCWEVNSAKGLSRDAGGDNQGLISMIKYTIQKYNADPTKVFATGSSSGCMMTNVLMATYPDVIAAGSCYSGVAAGCLAGSPGASPQTADPKCANGQVNKTPEQWVATVKAMYPGYNGSYPRMAVWHGTADNFVNYPNLSEELKEWSGLLGVTFSKNVTNTPQSGYTQMVYGDGTKLVGYSAQGVGHTVPVHETEDLKWFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEX0</accession>
    <name>A0A9W8YEX0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">dual-specificity kinase</fullName>
            <ecNumber evidence="1">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LKH1</name>
        <name evidence="14" type="ORF">N0V83_003655</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEX0"/>
    <dbReference type="OrthoDB" id="283111at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14134">
        <property type="entry name" value="PKc_CLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000612">
        <property type="entry name" value="Serine/threonine-protein kinase AFC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051175">
        <property type="entry name" value="CLK_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="295"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="47"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="113"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="159"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="225"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4373360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="674" mass="76554" checksum="A2B8642575C19060" modified="2023-11-08" version="1">MSTPSTATATLPPHHQFYSHHQTYQPNMSNSQVANGSARIGNSLYNGYASGTSNSDGRRTATAHSRLPPQLPPISQAPALDMDSGSAGAAQRRRNPDWADFYKNGLPKEVIVIDDDDEDPPPPPPRQPALTRTATNGSARHTDKKRKTTASTAYDPVYNQHTSYSTTQTPYYDSPNHSISTDRTTSALNTTAPTSLGSQISNGQHISPLDNTAVGQKRKRTRAVVQDEAKQAKRRELDHDDNPFSLYQPPPNPVIKAKDVYVQVVPDKSFTKDQKVDDEDGHYIVIPEADLTERYQITKLLGQGTFGKVVQAYDRRKGTNCAIKVIRSVPKYRDASRIELRVLSTLASNDKHNVNRCIHLRDCFDFRNHICIVTDLYGQSVFDFLKSNGFVPFPSSHIQKFAKQLFTSVAFLHDLNLIHTDLKPENILLVNNNYQTFTYNRTVPSSSTTVNRTARHRKVLLDPEIRLIDFGSATFNDEYHSSVVSTRHYRAPEIILNLGWSFPCDIWSIGCILVEFFTGDALFQTHDNLEHLAMMEAVCGGKIDREIVRAVYKQDRSSSRNSANSAARYFKSYKLDYPNAETNKASKKYVKAMKKLPETIPGHTDFNRQFLDLLRRIFVYDPKKRITAKEALQHPWFKESLMDDGTEAHKIRLEREKKARRVEEERRYREQRAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF05</accession>
    <name>A0A9W8YF05_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBM-cenC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF05"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="20197" checksum="BE32E083AB1659F7" modified="2023-11-08" version="1">MVLSSKTSSSTTSRPASSTTATTCFATPTGNNYITNPGFEQGSSSWSTSTSGWGTWSASGTGLFTTDNAHSGTSAYRGTTDSIWLTTYTLYQTVTVPCGAKVSVSAWARSLAPKYSGLAAFTLTFDGWVVDAALSTSKESGAWFQLSGSTMTSSGSTSRVLRLDVTTVGYKGAVFAADDFKVAVVAGPGGQQVCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF12</accession>
    <name>A0A9W8YF12_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1989 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003715</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF12"/>
    <dbReference type="OrthoDB" id="504708at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018959">
        <property type="entry name" value="DUF1989"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31527">
        <property type="entry name" value="RE64534P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31527:SF0">
        <property type="entry name" value="RE64534P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09347">
        <property type="entry name" value="DUF1989"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1989" evidence="2">
        <location>
            <begin position="49"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="325" mass="36549" checksum="F2D9E96B5B9B581E" modified="2023-11-08" version="1">MASNSRDNIPPAPAYFPTSADSPLHPPKELYDGLRGLTKDQLTKKKEFVIPPRSGKAWKVGKGEIWRLSTPEGPQVGDLNIWNQHDPRERFWAARTRQLQSSHMMEGDRLWSNLPFMRPLCGIIRDGCEIRDAPTGEGKGDGKNGGREEERDERGGVTKWGGRCHDLLGTRCDPYVSNMLTGASYDYHCHSNLVRAVLPFGLTEFDVHDVLNVFQVTGLDSQGRYFMEASPATKESFIDFFAEQDLLCALSTCPGGDLSAWGWHQADEGAGDDSKPDMKSTCRPIKVEIWQLKDEFKEKVLKGWRNPERSQYEGVHGIDIPEGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFH3</accession>
    <name>A0A9W8YFH3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_002001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFH3"/>
    <dbReference type="OrthoDB" id="10069473at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099122">
        <property type="term" value="F:RNA polymerase II C-terminal domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17003">
        <property type="entry name" value="CID_Rtt103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000030">
        <property type="entry name" value="DUF618 domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047883">
        <property type="entry name" value="Rtt103-like_CID"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12460:SF0">
        <property type="entry name" value="CID DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12460">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="3">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="291"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="230"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="315"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4374923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="435" mass="47376" checksum="9C24001517EB3802" modified="2023-11-08" version="1">MAFTEDSLKAKLSSLNETQEAISTVGQWILFHRRHAERITQVWLQRMRESTPNKKLVLIYLANEIAQTSKMRKKEDLLRAYEPIIAQATAIAYKGSPHETQNKIRRVVEVWRSRQIFSMPVQQDVEKQIDEVDRSKSSRKPALGGSLFSGSSIPPDLAPVAPLATSLQKVDLNAKPAVVTANQDYEKLTNPNAAILSPPMHAASLAALVKKLAVAEGAVAESIKARESLVAGLEKLLDATREKLVQDEAQVSDLRTRKDAIESRKREVEAAILKGLSAAETNKISAAPLPVAATPPSAQERPQVEELTPPPMESFTPTGSPQQAPTEAFEPVPDLGDDVMPEAVSHPVEPETAPAPGQHTTQPEYATAINDFVPPVPEDLLKSLQHARPGAEGGVYGQNAYQNAYKKRKMSRSAAEDEFAAFAGEGIDDNLGELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFV9</accession>
    <name>A0A9W8YFV9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000175</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFV9"/>
    <dbReference type="OrthoDB" id="2152029at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF8">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="93"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="333" mass="37054" checksum="4A68C95E44A728E1" modified="2023-11-08" version="1">MLDHDARQKSLIRVITRDERSFLMTVVQLVVRTFRSQLSSREASKHEDGSVRIDTVKAKLPGCTLSERTVCGIYIYDIVPRVKPAKDPKKRIYYLAGGSWQTPPSGQHYHVCAKMARDMPDTRVSIVSTPLAPNNPAPGTFPICMTLYRELMAQAQEAGERVILAGDSSGANMILCMTLEALKEDNDNEKTDAASIPHPSVILAVCPSTDLTRDNPDIQKIAPKDPLLTPDIINKTAKAWHDDWDAADRRVSPINNDISLLAKRGIKVHGITAGCDVLSPDGIVFRGRCAEVGVEGEWVHWEKQMHCFILTMQYGLPEAKEAVQWMVDILKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG53</accession>
    <name>A0A9W8YG53_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001726</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG53"/>
    <dbReference type="OrthoDB" id="433474at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF600">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_1G02580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="67"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="239"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4376442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="432" mass="48199" checksum="594AD6F9C4648630" modified="2023-11-08" version="1">MDFWRVLMGIGMMLHRLARPRPRHPAFHKDIEATVSPLKGKFRLNFYVPKDYNRHKKLKGVKRYPVVINFHGGGFVLGTAHDDARWCNIVVNEVGAVVVSVDYRLAPEHPFPTAVEDGADAILWLAQHADELMLDPDRFAVSGFSSGGNMSFTVPLCLQGELFERTASGANIVDGRAGSVPNAVIQPPPAYGAHLTPNGSRVPLVRQKSRLDRIAMLRQTGASSLSLISSYKDGSAVSVMTEASNAIVKIRGIVSFYPPTDYTQTRAQRRATCTRADQQLPAVFTELFDDSYLQPPSLDLAHPWLSPGVAPKRMLEALPDDIVLFCCEWDMLLAEGERFRDILSKDLGKRVHYHCVPGVPHGWDKAPNPLRESPGARQQYLVACKELKRMFDIEDDDDDDEHEHGHGKDLPCRQYHKSVDLGRHPLAQQQTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGI1</accession>
    <name>A0A9W8YGI1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Alpha-acetolactate decarboxylase</fullName>
            <ecNumber evidence="4">4.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_000047</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2S)-2-acetolactate + H(+) = (R)-acetoin + CO2</text>
            <dbReference type="Rhea" id="RHEA:21580"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15686"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="4.1.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Polyol metabolism; (R,R)-butane-2,3-diol biosynthesis; (R,R)-butane-2,3-diol from pyruvate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alpha-acetolactate decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.5" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGI1"/>
    <dbReference type="OrthoDB" id="509395at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047605">
        <property type="term" value="F:acetolactate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045151">
        <property type="term" value="P:acetoin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.80">
        <property type="entry name" value="Hypothetical protein, similar to alpha- acetolactate decarboxylase, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005128">
        <property type="entry name" value="Acetolactate_a_deCO2ase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35524">
        <property type="entry name" value="ALPHA-ACETOLACTATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35524:SF1">
        <property type="entry name" value="ALPHA-ACETOLACTATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03306">
        <property type="entry name" value="AAL_decarboxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117856">
        <property type="entry name" value="AF0104/ALDC/Ptd012-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0005">Acetoin biosynthesis</keyword>
    <keyword evidence="6" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4377224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="209" mass="22900" checksum="7E9A8C8C90EF7166" modified="2023-11-08" version="1">MPASIPNDIFQFSVYSALNAGFNTGQPRTADLTTHGTDGIGVYEDGTLMLLKDGQAHSINKSSSARPAPMDARLPFAMVTIYQPSFKLKIPWITLESLDELISSSELGPAKGVNTLIPFKIAGRFEFVDFADGAGQKKVDGTVSGFVIPAWMKHMSGERIHAHFLDASEEVGGRVVDFRMNEEGVLSYAKCGRFHLGFPQGEQWEEMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGL4</accession>
    <name>A0A9W8YGL4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001630</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGL4"/>
    <dbReference type="OrthoDB" id="3793045at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058525">
        <property type="entry name" value="DUF8212"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10622">
        <property type="entry name" value="HET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10622:SF10">
        <property type="entry name" value="HET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26640">
        <property type="entry name" value="DUF8212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="2">
        <location>
            <begin position="21"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="DUF8212" evidence="3">
        <location>
            <begin position="226"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="583"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="675"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="599"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="693"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4376347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="725" mass="80453" checksum="69B351D47B8AB8CD" modified="2023-11-08" version="1">MRLLHTSTLEVEYFLQSTPKYVILSHTWGEEEVSLQDIQSGSAPSKKGYAKITACCKKAAEDGFSYCWIDTCCIDKTSSAELSESINSMYMYYKNSSICYAYIADFDIERGHSPSLNDINAPHGLGSQRWFSRGWTLQELIAPPIVEFYDVNWKEFGTRLSLQQQLTAITGISKFILQGGDPAICKIAVRMSWAAKRKTTRIEDEAYCLMGLFSVNMPLLYGEGSRAFRRLQEEILRVEEDYTIFAWAPKESLESDDALPAVSRGLLAHSPSDFAEFRAENHTQAPLMSPLWPLEDLSRDHFSIFPPSVDQVPPYLTSRGLRITLPVTLVTLQNNCQDIYACLTLIYPPGGQMHMLCVKLIRATASEERYIRQAGVLLVVLPQANRGHFEYRSIYVEQPHCLHSGPPPWDIDNAEHQASSFDLSLLVVKLKANFALPATFNTISAVNSDAITHLRYSKQTSASDPTTREIVDAISSNIELNYHGVNELTYSFDPVGSPGHGEINFVCNTITPPPRYILTFAFANHPTTTFKVSLNIPSGTPPTCTALSTSPSYARLHAQWHHPSSETLGSLHKTDRVTLYVDLEDSSPTPSPSPSPTHSDSDKEKQKDKEKGTEVPVTHIQVDISIRRIAPPSPHLFSQEYETRFQNRFELSIELKDVTPRGSMGAVGVNTEAALRSKAQERGEGSGGGSGGGREEGSIVKEVERVDSWEQISRSGTGLQEGGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHE7</accession>
    <name>A0A9W8YHE7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_000502</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHE7"/>
    <dbReference type="OrthoDB" id="3934656at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF168">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4377674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="399" mass="45124" checksum="1E29786DE02F8066" modified="2023-11-08" version="1">MNAVRSPYRRAPWYCHAARFEPGKDNVFTDCDNDSHDARRKKMASGVSNIEDRKYSGKENPTLEPSIDIHVKELVELVRKYAAPTSSARTSTPMDLAKKIPFFTLDVISHVGLGEPFGNLKANEDLKDYLKSSEEGLKIANTAFAMGIAWLREIPIIGPAISPSEKDASGFGRMMAEARKIIEMRKSKSIEGKSDMLASFTRNGVSGEDLFQETFAEIDGAVRSGTATTGVVSDAQARGVPYLVAVVREALRVHPPVVNIFSRITPNEGDTVTIDEREYHIPGGTLIGYAAWTMHRDNRSIYGDDSHVFRPERWLIDESIQEEKERLTRMTRTNDMIFGYGRWRCLGRNIALIEIHKCIFELLRNFDLALTNPHQPWHVFNSMGLWEIRDMWVDVTERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHG9</accession>
    <name>A0A9W8YHG9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">LPXTG-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_001225</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHG9"/>
    <dbReference type="OrthoDB" id="5426678at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051694">
        <property type="entry name" value="Immunoregulatory_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15549:SF26">
        <property type="entry name" value="AXIAL BUDDING PATTERN PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15549">
        <property type="entry name" value="PAIRED IMMUNOGLOBULIN-LIKE TYPE 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040926009" description="LPXTG-domain-containing protein" evidence="7">
        <location>
            <begin position="26"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="314"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4375946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="38626" checksum="248645439D0B071A" modified="2023-11-08" version="1">MVRSRSRPTTSGFLLLLVALSRSSALEVSPDSPCAPKCLDNPNGNVSDVDSSKTFNYNLACHDWEYMGDNSTQVGQKFKDCNNCLKSSEYIDTASDERDVEWFLFNNRGTVDWCLFGRFDNEKDKNITGSSIYKQCKNDCDAIYTSADYTIKKDPASYSFCDYDGNFTADADTCLSCLRKQDGLSILGNILETVQDLCKQKPGRVYDFPDNQEIYTTTPITLSATSSTSPSASPSPSPTPSSGLSTGGIVGIALAGVALVLGVILYFLRKRKNKNKSKQMAVAQGENGYGNLNTHGPGYGAPAGNYAYNPMAQSQKERYAHQPPIANVEQPGPAELGAQRPVTELPGGDVPGPIRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHH4</accession>
    <name>A0A9W8YHH4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHH4"/>
    <dbReference type="OrthoDB" id="5337308at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4378190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="310" mass="35171" checksum="47A171A84457DA65" modified="2023-11-08" version="1">MAVKSDLQPLYQDFQDALASKPQDEGKKVAWLRDPMSLTSKERASFERAFAKGSWLLQLMLSNDHQAGLMFKTPQASDESAFLSSSDLDKWGWRGARNTAFTENWKLISRSVCHVLRVTGLYSTSVDRDNDDHEKSVVDHHYEIGQSIEDTWYSWARALFVQYIDADAGIIVALNNESPSETAHHCGLQGVPSPDLRFWSDVAYLQWSLRASDTSDLKYVLRHNVVNEFTRSILNIITLKNQILIEEWPGRTYDVTSEEGQTLLGTPNGSGVAFLLIQHKKQLGHKAVGKITVFRVGWENMLLFHIKNVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHZ3</accession>
    <name>A0A9W8YHZ3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription initiation factor IIB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">General transcription factor TFIIB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SUA7</name>
        <name evidence="11" type="ORF">N0V83_000698</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHZ3"/>
    <dbReference type="OrthoDB" id="25790at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20551">
        <property type="entry name" value="CYCLIN_TFIIB_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000141">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000036">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="10">
        <location>
            <begin position="36"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4377868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="366" mass="39754" checksum="3A2C55DDC83435BB" modified="2023-11-08" version="1">MAAMYAPTGRLLSPGEVYEEVSAPVKEEEWQEDLNVVLICPDCQDNPPILIEEFSSGDTICGNCGRVLADRIIDTRSEWRTFSNDDQGNDDPSRIGDAANPLLHGSQLSTEIGFGDGGIRVRELSRAHNKSNHDKTNKNLQAAYGQITTLCDSQNISRPTAETAKMLFRQADDAKLFKGKSQDAIIAGCIFIACRQMKQQRSFREIFKMTNVSKKEVGRTFKALEAFLQRIQKEKGGPMVAGNGAVLDANNYLTGTATSAENLMPRACAKLGLPPHVQIVAENCAKVVTDAGVAAGRSPLSIAGAVLYLVSHLMSYPKSPKEIGAAVEVSDGTIRTAYKLMHAAQDKIIQEEWIKKGGDRSKIPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIL5</accession>
    <name>A0A9W8YIL5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">tRNA (Uridine-2'-O-)-methyltransferase trm7</fullName>
            <ecNumber evidence="8">2.1.1.205</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TRM7</name>
        <name evidence="8" type="ORF">N0V83_000856</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.205" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIL5"/>
    <dbReference type="OrthoDB" id="289250at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000220">
        <property type="entry name" value="CAMK protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF12">
        <property type="entry name" value="TRNA (CYTIDINE(32)_GUANOSINE(34)-2'-O)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="7">
        <location>
            <begin position="21"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="86"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4378026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="329" mass="35998" checksum="63D25BBC327139D8" modified="2023-11-08" version="1">MGKSSKDKRDAYYRLAKEEGWRARSAYKLLQLDEEFNLFEGVTRVVDLCAAPGSWSQVLSRVLIKGEKFGRAGLQEKQAATRSYVLGQSKPSSPLSNAEKDGTTVSKEEGEDKSPKPREGHHTLLPVDLVISDGAPDVTGLHDLDIYVQSQLLWAALNLALCVLKPGGKFVAKIFRGKDVDLLFAQLKIVFKRVRVAKPRSSRASSIEAFVVLPVPPPKEAAKRGEESKAARRVRDDGVVELDLGSESEDGEDDEDAEEGGQRWIAPFLACGDLSAYDSDATYNLPKDRVSLDPVQPPTAPPYKRALEMRKLAGGAYGKTKGRESKDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIN2</accession>
    <name>A0A9W8YIN2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIN2"/>
    <dbReference type="OrthoDB" id="3786129at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="266" mass="30676" checksum="C92CD988C744D366" modified="2023-11-08" version="1">MDSQPQTERGDSVTILDDEQLSESLRSYSDHATRRTAAPSPSSAFPPRPDSVLNNTISNDNNTKSRNLLARVWYHIRAFLRPSRKAVWEKNTEVSIVMSNNKEITRLAHVLFDPQCLGDLISTSFLRNFPGLQYERSEQRFGRTILSSPVPSIGTVDIRWHHEKAKNSTSRSKYSQTRIDNTTCHVVDSKEFDLIIGRPTIDRLELFIANRRLVAPFFRGPNTGIDPKTIEQTEQTAELVRGQAEQEQRRREEERKEKEQRERATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YKA4</accession>
    <name>A0A9W8YKA4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit L</fullName>
            <shortName evidence="4">eIF3l</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_000667</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YKA4"/>
    <dbReference type="OrthoDB" id="15082at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03011">
        <property type="entry name" value="eIF3l"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019382">
        <property type="entry name" value="eIF3l"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13242">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13242:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10255">
        <property type="entry name" value="Paf67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="PCI" evidence="7">
        <location>
            <begin position="259"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="477" mass="55554" checksum="E6493027E20FC66F" modified="2023-11-08" version="1">MSLRERPQFQNGEAARRVPDDQSDAEEEALVNDYQEQVQYSGMEDLDRMPSISSNQDIHAQLQAAATPLEFQATLETKFASYDNYCNLFHYILNSEGPVDLEVPNYYWAWDVIDEFIYQFNSFCSYRQRVAQKNDNEDEITLLRENPNTWGCYSVLNVLYSLIQRSQISEQLGAMKRGEDSNAYAGEYGSRPLYKMLGYFSIIGLLRVHCLLGDFSLALKTLDDIELNKKAMFARVMAAHFTTYYYVGFSYMMMRRYADAIRMFSHILVYVSRTKNFQKNAQYDSITKKNDQMYALIAICVAFQPTRLDDTIHTALREKYGEQFNRLQRGGPEALPLFEELFRTACPKFISPTPPDFDNPEINIDPVEHHLRIFMDEVKNNMMSPTVKSYLKLYTTMDLKKLAGFLEVEPEKLRCWLLVNKQRNRQTRWTEGGLLEGDVLHSSDLDYAMEGDLIHVSEAKVGRRLVDWYLRNLARTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YKH8</accession>
    <name>A0A9W8YKH8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Snorna binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_000787</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YKH8"/>
    <dbReference type="OrthoDB" id="409543at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="512"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4377957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="638" mass="72046" checksum="289E49AA3DC40549" modified="2023-11-08" version="1">MAEGSKLRAVLTLLKNLTLAALLYAAIFGIINIVSDPKSAAKGKLGIPAFLDKTGPTELIETTKAGIKVKFAGTSKTVTINPHANVFNRPVTAVKNRDDYLGPRPHVDTEADMHMLVEECRGTYTGLEKMRKPYDCLKFFAEEQDRYYHLPDAAERASAQDPRQADYTDADGQGKTLESYVSVEDAVAANGSSIGSCPGPIIPYHVYWTGPATWRVEVFIKSYLYTQNLACSRLWLWLDSDRSPNAVHNMLNKDALFARFLPLVERGDIVVKAWNFPSRIPLATDKEDKNGFDYYSKPGAPDAKGDKAVAEDIVEDKDGQQWLVLTPRQMTFLPVAVSDAVRFIVLHIYGGAYFDMDVLMLRDMRPLLLPKEHAFAERWGAHPHPGDYNTAIMSLSANSSLSSYLLYGGIRMGVNFHPRVLGRMAWKDGRDQEFLMFETAAFDPIWTEFNWDREGRCTVPCLRDYSAVFRGKTGAIKDEWESYAGPQLETINLEEAQRKELKMRMNAPVMKRAHEDEENMPTAPVEPSSKQAEKGAHADSFQATLDEEVELRKAGVVSDYVLSEDKFPPNNRTLENFFRGSWTYHIHNQWLKHPEPSSWISVLEHAQDGFFAGKRTNPYGEKWTGPSLMPYNYWPEFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YKS4</accession>
    <name>A0A9W8YKS4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)</fullName>
            <ecNumber evidence="4">5.4.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PGM2</name>
        <name evidence="14" type="ORF">N0V83_000939</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-seryl-[protein] + alpha-D-glucose 1-phosphate = alpha-D-glucose 1,6-bisphosphate + L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:68748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:58392"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>alpha-D-glucose 1,6-bisphosphate + L-seryl-[protein] = O-phospho-L-seryl-[protein] + alpha-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:68752"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58392"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23536"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="5.4.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.2" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YKS4"/>
    <dbReference type="OrthoDB" id="2291at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004614">
        <property type="term" value="F:phosphoglucomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.50:FF:000002">
        <property type="entry name" value="Phosphoglucomutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000005">
        <property type="entry name" value="Phosphoglucomutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000006">
        <property type="entry name" value="Phosphoglucomutase PgmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045244">
        <property type="entry name" value="PGM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005737">
        <property type="entry name" value="PRK07564.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22573:SF2">
        <property type="entry name" value="PHOSPHOGLUCOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22573">
        <property type="entry name" value="PHOSPHOHEXOMUTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24947">
        <property type="entry name" value="PGM1_C_vert_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="8 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="12">
        <location>
            <begin position="14"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="13">
        <location>
            <begin position="275"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4378107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="533" mass="58216" checksum="3208B3279BAF90AA" modified="2023-11-08" version="1">MSVRTVELKPYQDQKPGTSGLRKKVKVFQKENYSEAFVASILQSIPEGVAGSFLVVGGDGRYWNPEVTQLIAKIGAAYGVKKLLIGQNGILSTPAASHIIRIRKATGGILLTASHNPGGPEEDFGIKYNLANGAPAPESVTNKIYETSKTLTSYKIADIPDVDLSKIGTQKYGDLEVEIIHSTEDYLKMLKDIFDFDLIKAFLKQHSDFKVLFDGLSGVTGSYGILGGHHPDPNLVYAKSLVDAVDKNGIHFGAASDGDGDRNMIYGAKTFVSPGDSLAIIAHHAELIPYFKKQGIYGLARSMPTSGAIDLVAKKKGVECYEVPTGWKFFCGLFDSDKMNICGEESFGTGSNHIREKDGLWAVVAWLNILAGVGQQTGTTPSIASVQKDFWKTYGRTFFTRYDYEGCETEGANKVTSHMKELITTKKDDFVGSTISGRKVVEADDFSYTDLDGSVSKNQGIYVKFDDGSRIVVRLSGTGSSGATIRLYIEKHTSDESTYDLDAQEYLKDNVKLATDLLKLQEYIGRTEPDVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHS9</accession>
    <name>A0A9W9CHS9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010114</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3248909at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021840">
        <property type="entry name" value="DUF3433"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37544:SF3">
        <property type="entry name" value="SPRAY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37544">
        <property type="entry name" value="SPRAY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11915">
        <property type="entry name" value="DUF3433"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="594"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="707"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="750"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="824"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1322"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="676"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="676"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1423" mass="156374" checksum="886EB37D6297A3AB" modified="2023-11-08" version="1">MNQASHSPVSPIAPISPTAPAARTGVAQAPVTPTAVSTSNRNGSWLPFTFRWYFLLLPTILSITFGIVIIILISYSQRNHGLGPDDGSSAILFGWRFTPTLLAVLYTQLTVIIFDDAKRTEPFARLAKAPAEGATANGTVLQKSRAWWSIFSDALFKRKVVGKTSWCLVCSALINVLALLAISPLSSAFLTSEEVLISNVSSFSRIIPRDGVGLPLVANRETYFRTMAALMRNISTSAWITDSSLTLPFWPSSEELQLGSDFSSSPGTWKTNTTTLYTGLGCEAMTLQSADLKREFYSGAYDFTGKGPYNGTQPMVTFVLSSNDGCKYSLSLHPGAHLAYNGGLVWSNTSNLDFTSGASLLIGNIAFGANLTSTSPYARLNSTEQCRDRDIIILATPWTKPVHVQNLGNPALSPNQTYSRSDKFQMKGLLCESNFTMESHAITASISTTAQTSVTNPSNSSINRVALPQALVATPGFQASALQDNWRHYFNTVAMSTDSKSGLNGYLKAGADARNRVAIPLFSGMGPLLGALYSFKLSAMLDDPNFAAQAARAKGRFFTESLRETLTNPEAVQAETIAGEATVVQERVVVITEIGITLAVLLFVSSLLLALVYWSSRLFQRPLHLRTDPSSTVGLSMLLSTQASSTSVFKHMNRAPTSSLHSVLRAETFYTLNGSLHESNSMHRPSTETGDGNKKSTRKWQPLVLRLRMLSALGSFLMLVLVAVLILNAFSAKSRLSQLAFVYEADVSKLGLSFSTFAPISIAPTLVSIIVGLWWDQLDSTFRVLQPYISMSREPTPFARGAGLTYRSKTWIGAAAKAARNRHFVLFTIAMGSVLCQVLTVSMSALFERRVDNVAQQMELNTTLSMRRGPVITDIEATNASQPLRHTWQVLDELYLDPPKNWLAGAAIQLSLNGSQLPWSQDGWSFVPYDLTNILSRTNSNNSENVLVHSSNITVQTSGIRARLECSPIEDIANTSSWLTHPDEDDFGDFNITGFKDFGPEDFYWLNRTMFDNTSSNTSVFANMNEIQCCSNGTNKDPQTAAIGYWSPTDAQDFPHVDKKWPIPFVTKWFTGRPIRLYEDGYDPELSDRPWQNLLLFHEAPSLQAAHCEPVIEVAEANVRVDITTKSVLSYELTGPVAAPDEAWSEIFTIHDISESNQHYSTSYMGPLNITTSYGILFMDAMFKASDPNVDSVLTTTSEQASDNAFVMRDQEHGINMDLMTYSMYTLANKNAEALLDYNVLVDHANRTFQTFFQHFVNNGLSVTEGGLAYQKINDESYQDLGRAVDINGTALAVKTYTVVDSNRTIQASVSNRVQVLHMNSVATYLSTAILIWLIGTTAAMMCLQRRYTRSMIRDVQLIADVLVLVAGSDNFLALIHDKGMALKKNKDIKTMLGWFKGADGEVRWGVEVVGGRDAVDWVKAPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHT6</accession>
    <name>A0A9W9CHT6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Long-chain fatty acid-CoA ligase</fullName>
            <ecNumber evidence="8">6.2.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">FAA4</name>
        <name evidence="8" type="ORF">N0V83_010264</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHT6"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020459">
        <property type="entry name" value="AMP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF83">
        <property type="entry name" value="ACYL-COA SYNTHETASE LONG-CHAIN, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00154">
        <property type="entry name" value="AMPBINDING"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="7">
        <location>
            <begin position="110"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4363144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="697" mass="76034" checksum="79A9F97E62841444" modified="2023-11-08" version="1">MVQDLKKLMPQARASRAGPYTTEAADAQKVDGETIPRRNLASKDGLRATPTDGVETLYDILRYSSAKYGNAKAAGSRRIVNIHEETKKIKKMVDGKEQEVDKKWQYFELSPYKYKSFVEFEKIALSVGSALKNLGYGPQDRMHLFAATSMQWLASAHGAMSQSMAIVTAYDTLGEEGLRHSMLQTQAKIMFTDPELLPKLVNPMKDAKDVQVVVYCTKNDPKQKDIDTLTKAHPHLKVISFEELVKLGEENPAEPIPPKAEDLACIMYTSGSTGTPKGVMIKHRNIVAAVAGVDVIVGKYLGPGDVLITYLPAAHILEFVFENAVLYWGGTMGYGTIRTLSDQSVRNCAGDIRELKPTVMVGVPQVWETIKKGIISKVESGGAIKSNMFWGAYAAKSFLLGSGLPGSGVLDSIVFNKVKEATGGRLRICLNGGGPIAKETQRFISLAITPMISGYGLTETCGMGALMDPLAWSEHALGEMPASVEMKLVDFEDAGYFSTSTPPQGEIWIRGAAVVDGYLNMPEETEESFTSDGWFKTGDIGEFDSQGQIRIIDRKKNLVKTLAGEYIALEKLESVYRSAPIVANICVYAAEDRQKPIAIIVPAEPALKKMAAAEGVKGDHLEELVHDKKINSAVLKQLQQAGQKGGLAGFELIEGVVLADEEWTPQNGLTTAAQKLNRKGILKHYEKDVDRAYGKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIA3</accession>
    <name>A0A9W9CIA3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Complexed with cef1p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CWC15</name>
        <name evidence="6" type="ORF">N0V83_009896</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIA3"/>
    <dbReference type="OrthoDB" id="30179at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006973">
        <property type="entry name" value="Cwf_Cwc_15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12718">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12718:SF2">
        <property type="entry name" value="SPLICEOSOME-ASSOCIATED PROTEIN CWC15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04889">
        <property type="entry name" value="Cwf_Cwc_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4363600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="237" mass="27161" checksum="06EC851D16F64875" modified="2023-11-08" version="1">MTTAHRPTFDPARGKEAQRGPAYHQRLLPAHTTLKHRQPGQGGDADYVKRDLRAELLEAEARHAAKKNGTYVEPEATGESGNAPKRQIEDVSAAEEGEGDEADQKRRRIEMLEKFREIDADDSSSESESSDDDDEDEEDETAALMRELEKIKKERAAAKAKEEAERAAEEEEQREIDVARGNPLLNARDFAMKRRWDDDVVFKNQARGTEDKNKKKEFINDMLRSDFHKKFMSKYIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIY1</accession>
    <name>A0A9W9CIY1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007769</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIY1"/>
    <dbReference type="OrthoDB" id="4850726at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF64">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26639">
        <property type="entry name" value="Het-6_barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="1">
        <location>
            <begin position="269"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="375" mass="41508" checksum="908682C104A15DDF" modified="2023-11-08" version="1">MSALHIAPDYSLTPAQVYKDMTLRYIARFQTLDLFRSCDLETCPPGFSSFVPDYASPKSESRLFTVHAHAGSQQSYVGTGDGGIVVRGVNVDGVCKVSPARKPLAVRTHEGDNHADIIATYRAWEPPGLMESPYPLGGTLLDAFVLLLSNGFCKDAYYDGHLPTFAESCDTFTACVWSGGDAELRTEPSHRAYVNELTSDRRGEVFFTTEKGYLGVSPASVKAGDVVAILAGASVPIVLRPQHTLDTRKYQIVGPCYIQGLMLGEGLLGSLPPGWMGVMHETRKWCFQTTDSALRTWEDPRLWSLPGHWQAHFCDIENKGESCQGACEIEYVRGARLADRWFLDMMSGERRREDPRLTIEGLEEGRVVLEAFELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ40</accession>
    <name>A0A9W9CJ40_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008795</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ40"/>
    <dbReference type="OrthoDB" id="3775766at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040936108" evidence="2">
        <location>
            <begin position="19"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="148" mass="15657" checksum="5409BE07E54F2D46" modified="2023-11-08" version="1">MRFSAISLFAAGLVVTMAQDVGKVALCNDCWQLGTGAPTCSDNNYIHWGPEDGVDGECDDPPSVFDSPCEADSSSNIETPLGEGPYTPEPDCDANAENGTLMGTIVGLRSGGPGGGVFKYNCYKELDTFTNVCGFTYACNIYLICRQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ82</accession>
    <name>A0A9W9CJ82_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome b-c1 complex subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">QCR7</name>
        <name evidence="10" type="ORF">N0V83_008811</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the UQCRB/QCR7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ82"/>
    <dbReference type="OrthoDB" id="425749at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1090.10:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1090.10">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003197">
        <property type="entry name" value="QCR7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036544">
        <property type="entry name" value="QCR7_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12022:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12022">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 14 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02271">
        <property type="entry name" value="UCR_14kD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000022">
        <property type="entry name" value="Bc1_14K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81524">
        <property type="entry name" value="14 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4365193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="131" mass="15336" checksum="BD7C24C371E13E2F" modified="2023-11-08" version="1">MSAPSLAPYIAKRPWLQRWMKPLSNWYCNAAGYRQLGLRADDLLPEENDVVQTALKRLQPKAAYDRVFRLRRALQLSMSHQLLPKEEWTKPDEDVPYLQTLIEEIETEMAEREDLESLVVSKRKRNAESGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJQ9</accession>
    <name>A0A9W9CJQ9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006854</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJQ9"/>
    <dbReference type="OrthoDB" id="3596604at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="228"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="566"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="677" mass="76552" checksum="385913F1E9E3AB15" modified="2023-11-08" version="1">MEPNRAFRFGTDDPSPNAPSIPYDPPESYNGGDIAMQNVETSYTNTSPVASPEIIESKGGVYTTIGSPSSTFDVEEGHHHHHHHHHHVKRFDHNESRKKLFKTGASKLLITIFFSAAICLCLKAWEGFRKPVVLSKNDVRIFNALLIGLSLCLGLNILASLKHYASTFRWSFLTRRYVSLEVFDLILHLSSLAKVTKLMIISSPGFRGRKFWRKFRLFKDVRDDGTKWMWLACLLWLSINIGSQILIALLSLFWPMSNSEMPLLTYGNVTVADLNTWYVQTNPEPDTDSMANETSLETAWMYGMEAMVYPEFATNDVQRDFSNMPGTPLYKDDGSYSYRFFNRDPSHQYTNYLVSSREISAVTTCEQLEVRGNGTVIDSDEEVKEFGSMYIEGKEPGKDWDKYAIPEQASGAITWIASVYEFCGPRCTNFTVLQYGFDDRVKTTSLFLCNSTLANVTKVHATDDVTTHTPADSSAIYGSDSFARIAAGAIGWTGIPWNNWIDRQTRSYSQGSKWSPAHVVSKQEVEDMLMRFTIGAVAAFDDHGIRYNMSSQRVVPTQGQQLDVDWSYIFSILFSIIGIQALALLLLVIFANRTIVRDDSFFSIAMLLKGVVERIGEGAGGRNMSGEEIKEHPRLKWKKIRYDYREGRDGEPNMVDIFFEGKDLKDARKSWAPGSYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJR8</accession>
    <name>A0A9W9CJR8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008009</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJR8"/>
    <dbReference type="OrthoDB" id="194468at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050287">
        <property type="entry name" value="MTA/SAH_deaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794:SF11">
        <property type="entry name" value="AMIDOHYDROLASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794">
        <property type="entry name" value="AMINOHYDROLASE SSNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="2">
        <location>
            <begin position="59"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="505" mass="54518" checksum="F87181435A1C18B5" modified="2023-11-08" version="1">MSSSRRIILKNGVVLLHDQKNQVVPTKTSILIENSKIANIAQDIAAAEGIEVIDCTDKIISPGFIDTHHHGWQTQLKGRHANEQLLEYMVTGNSQHHQYSPEDVFYGQLAGMLEGIAAGTTTVVDHAHITLSPDHARLAIAATASSGIRSVFCYTPVMAVKQFNPLGFIQNPFEDWVMQTFHQIADHGAYGSGRVTLGFAFDLFFLPAEMIKGIFAQVKEKGVKTITCHGSVALGMSLVQNLKRLDLLDERIIISHGGTIKKADAELVKAVGAFVSSTPSTELQMAMGRPYSFDASFLDGGVIGDSIGLQDNGSLGVDCHSCTAGSLLAEAKLGLQNARNHFNEFHMKQGKIPRTVPESLSVQSAFNLATIKGAEAVNMSERIGRIAEGYQADLVVFDGLSPSMVGAAQHDPVAAVILHSSPADIETVIVGGVVRKREGKLLRVPLDGCAKETAGTDVLEWSSIAKEIIKSREKMQDAIDKIDFSEAKSTLLKVFHVDEGRLVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJZ0</accession>
    <name>A0A9W9CJZ0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">ATP-dependent DNA helicase Hrp3</fullName>
            <ecNumber evidence="16">3.6.4.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">hrp3</name>
        <name evidence="16" type="ORF">N0V83_007096</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="16"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18659">
        <property type="entry name" value="CD2_tandem"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056302">
        <property type="entry name" value="CHD1-2/Hrp3_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025260">
        <property type="entry name" value="CHD1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF14">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13907">
        <property type="entry name" value="CHD1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23588">
        <property type="entry name" value="HTH_CHD1_Hrp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01176">
        <property type="entry name" value="DUF4208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="16" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="13">
        <location>
            <begin position="266"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="13">
        <location>
            <begin position="366"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="464"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="766"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="22"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1030"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1310"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1561"/>
            <end position="1580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="73"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="237"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1030"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1052"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1078"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1315"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1331"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1348"/>
            <end position="1449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1464"/>
            <end position="1476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4367513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1580" mass="180925" checksum="E3C0E96EDB3933FA" modified="2023-11-08" version="1">MPSTPGHAASLPYTNGHISPVTVHVTAATSPPQHSDSNLSDAQEPAAADQASDVDAPGEDYDEDQDIAAASDSSEDVDAEGEPDGDYDSDSPPSEQAESHRARSSMSQESNRPLKRKASAEKDDYITQNPELYGLRRSGRARPSRRIVDSSDEDDSGSDQPRKRQRTASRKASNQPTPAFRAADSDSESDGYVGARRNIPTKKERQRQHLVAEGRAPPSQAEVRFSARRTAQVTNYNEDEEDDPFEEDEEETTPNYWVTAAEETGPMIDKILDHRPKEDTNLDPYYAQKKDFEYLIKWQDKAHYHSTWEDYQTAASYKGWRKLDNYFKGPVMSDMFYHARKKKEPEEFEQHMVAREAERESQLDFHVVERVIDSRDGEDETEYYVKWKGLTYEFCTWEPASLVSRLAQSEIDRFLDRSSNRPSSDTRESNLKTRSKFVKLDEQPDYIQYGKLRAFQLHGVNFLAHNWCRGTNVILADEMGLGKTVQTVSFMNWLRHDRRQDGPFICVVPLSTMPAWADTFNNWTPDVNYVIYTGREDARSIIREKELLIDGNPKRIKFNVLLTTYEYVLADWQFLSSIKWQFLAVDEAHRLKNRDSQLYEKLRSFSAPSRLLITGTPIQNTLGELSALMDFLMPGEITVDENVDLASEDASHKLAELSAAIQPYMIRRTKEKVENDLPPKSEKILRVELSDIQLEYYKNILTRNYEALNEGGTGHKQSLLNIVMELKKASNHALLFPNAESKFLKEDATKDETLKALITTSGKMMLLDRLLGKLKADGHRVLIFSQMVHMLDILTDYLKLRNYPFQRLDGTVPASERKIAIDHFNAPGSDDYCFLLSTRAGGLGINLMTADTVIIFDSDWNPQADLQAMARAHRIGQTKPVSVYRLVSKDTIEEEILERARNKRMLEFITIQRGVTDRQQKELNDKMSRAAAEPNSADDINNILKRRGQKMFEQSGNQKKLEELDIDAVLENAEEHKTEQAAGLTSDGGEEFLKNFEYTDVKIDLEWDDIIPKDELEAVKADIQQRREEEETQKLLEESAPRKRKAAAASAREQRAAKKRALEAARVDVDDDEPSEDENVKRDPKRPLNTKETRNLIRAYERYGSLDERGDEMLRAAKLMGRDMNIVKTTLGQVIAESEKLVKDEQTRLKNLEIEANRPLTKKDKKAVLFDFGMVKKLNAETIIDRPIEMRLLKEAVDAVADWRNFRIPDAIKPASYSCPWGAREDGMLCIGIFRHGYGAWVQIRDDQDLGLTDKFFLEEHRVDKKEERTKAEEKNAKSPGAVHLVRRANYLLTVLKDKAGVDPNAKRLMENHHRNNKKHHLNLARRPEKASSVSASPAPSGMVRKVASRDSERPVQRAHSNSENRHPDSRPDSRQYDKSTHDRPRHSDQYRPSDTHRKEYRNDRGSIDRRPQHPDRNGTPEIRRKPSGDVDRPRAKDERVHLPEERPRSHSQSQNHGVNRNVEPPKPKEKKPEDFIERKLRPVRDNLSRLKKATPKNYQKDAMIKVLKVELIAIGNFIRAETRDNAELEERLWAYTVNNYWPRQTVTVPAIKGMYNKMLSSEKPSASTPAINGQKNGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKI2</accession>
    <name>A0A9W9CKI2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKI2"/>
    <dbReference type="OrthoDB" id="5360701at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="337"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="441" mass="49167" checksum="C67FED874038CCA0" modified="2023-11-08" version="1">MKPGSLPMRARPRPRPVCQLCDYILQQPSRRRAFVAASTFKATSTPRQPARTHGIAPRIPTRSITTTPRKAQDVEIRDDEGLQTTERIAELKAELAEVEDQIRAIYNSPKVENEAVVLKALDGLERIAQKAIAIRSRQPPPTRTPIRQSSAGAILSGLGSEENVSTQRRTRTKQLGLDALPSPSYLSKLAEGLIKHEKVFVSPNVLAVYIHLQRLLARPKTIPEALYLYANKPVPVDGSSPPKFSHPSPKSAKQAIPADLADEALTAAIDAKDLSLALDVVDQTYRTPAWRRHRMFTKMGLPAALAGVAPLGIYMIAQELSVYSGYIDPWTFKLYAFMGLSTYVACTGTLGFVALTTYNDTHDRVVWRPGVPLLDRYLREDERAALDRIACAWGFKELWRRGDEEGEEWEGLRQWVLLRGMVLDKSDLMPGMNASTHARER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKM4</accession>
    <name>A0A9W9CKM4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycoside hydrolase family 105 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006991</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKM4"/>
    <dbReference type="OrthoDB" id="540611at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010905">
        <property type="entry name" value="Glyco_hydro_88"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052043">
        <property type="entry name" value="PolySaccharide_Degr_Enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33886">
        <property type="entry name" value="UNSATURATED RHAMNOGALACTURONAN HYDROLASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33886:SF9">
        <property type="entry name" value="UNSATURATED RHAMNOGALACTURONAN HYDROLASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07470">
        <property type="entry name" value="Glyco_hydro_88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040747699" description="Glycoside hydrolase family 105 protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="387" mass="43143" checksum="C8D0EB5387CCA026" modified="2023-11-08" version="1">MHFPALSLALLTCAGTTLAAANTTRPYSTWMASSFLSKGQAINNHYVASVIHEGIQKAATLHNDSSLLAYASNAVSSIVSSNGTLKGWNSTFYTLDDIRIGNNMLYFWDAEGRKEKKYEIAAKGLREQLNRWPRTPSGGFWHRAPIYKNQMWLDGIYMADTFYATYVSYFEPTNITAWDDIALQFDLIEEHCRNHTSNLLQHGYSEDKAAVWADPVTGASPHVWDRAVGWYFVALVEVLQVYPADLPGYSRLHEYLTTLADGLKRAQDVSGGWWLVMDEPYPGMKGNYIESSATAMFTYAYLKGIRIGLLEASYKDVALKAWDLMLDDFVQYETNGTLSWTGTVEVGSLSGNATYEYYVGVKKALNDGKGAGPFMYAATEIELAQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKT9</accession>
    <name>A0A9W9CKT9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKT9"/>
    <dbReference type="OrthoDB" id="3675419at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="429" mass="49267" checksum="12B606BF46F72240" modified="2023-11-08" version="1">MAEQLTSVPWSNNARKESLPQYPYIFRRVYFCGHPDEFIEVSRLPNYERLTHTLITLSDYDQCAKVRKSRQVYTLERCRRCALEHRQRHLESRRQSEKGTIRPITTYQGEVVRLEQTANRVKNGFELALMRFEKGYGAKQENEPHARVPESEHEGDWPLLEGSDGSVYGRALRFIGRPGDVQELSKNLADLMNPLRIYGGTHENIQASSEPGIIEEDHQRKYSKLRSLEDHIHTSDIDLQTPIRGEDQRETTGGSASQDIISQLLEEVTQCEYDYHDLDTPLSRFSSSSSNYFKETKPWWKLGKQPREDLQQEPKSPPNPHLHSIKGSLKIWKKHKKSASTSSEDKNRRWRSAGVQGLDGTRYSDEIVRESSKSLDLPRPGIARSDTVGEGKASELSFEIHRPFQRQASATVITNGATFSTVKEDAERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKW9</accession>
    <name>A0A9W9CKW9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006351</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4367996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKW9"/>
    <dbReference type="OrthoDB" id="192702at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021276">
        <property type="entry name" value="DUF2855"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11017">
        <property type="entry name" value="DUF2855"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4367996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="425" mass="46893" checksum="BC3466636225E86E" modified="2023-11-08" version="1">MSTSSPAIHVLDKANYSKHRVVTLPNEPLPPLAPSSLRLRPRILGLTTNNLTYARLGHLMGWYDIYPLPANTPAPYTDSSTYGRISAWGYADIVESTVPEISPGQTVYGYLPISTGLETVRIEFAKEHNDGQQTIKDEIIVLDEHRQHLWKIYNRYHVRPPLSTLEKTVGLDSLGWDALMQGLFGTAYNLNTFGFAWEDDGNRIHPSGKGEWTASDTDLRNATVVILNASGKTGMSFAYSLRHLRPKEHQPTTIIGIGSAASVSAIEKSGFYDKTVLISDARATAADIEESGTTGTTSRVVYVDFGGRVGASEAWKAALSSMPSVPFTLVTVGGEVEIQNPEKARKRLDSAASLNLVNASLLREKGIEAGGQKYFDEFYQAFEEFKTRVVGLQLRWGEGLSAWEMGWEAFVRDEVRADTGLVYRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKY1</accession>
    <name>A0A9W9CKY1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Triacylglycerol lipase</fullName>
            <ecNumber evidence="7">3.1.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TGL3</name>
        <name evidence="7" type="ORF">N0V83_007205</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable lipid hydrolase.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.3" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKY1"/>
    <dbReference type="OrthoDB" id="10049244at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021771">
        <property type="entry name" value="Triacylglycerol_lipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF44">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11815">
        <property type="entry name" value="DUF3336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="6">
        <location>
            <begin position="131"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4367620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="296" mass="32670" checksum="6575C959A9AC8395" modified="2023-11-08" version="1">MCPVVLTSYNADQPRNRRRQTAISKDYDHRLIHQRLSSIYEAQEENDILGLISLLRSGLVRNLGNITAPKLYNRAYAGTKLLIEDYVTQVAYAIENLTQYPTSPSSDTGLTNQAKLDVLHDTRQAFGRSVLVLQGGAIFGLCHLGVVKALHLRGLLPRIIAGTATGALIAALVGVHTEDELLDFLTGKNIDLTAFTNRPYRNGSSRSGWIETLLRRVKRWWREGHFLDVGVLEQVLRANVGDLTFEEAYTKTKRVLNITVTTSGGGGVPNLLNYLTAPNVVSLSLSTLPNLFTDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKY3</accession>
    <name>A0A9W9CKY3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKY3"/>
    <dbReference type="OrthoDB" id="506431at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="468"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="279"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="308"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="497"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="509" mass="55857" checksum="F1A6DEBC3DE95DC9" modified="2023-11-08" version="1">MAWFMRGVQSAVFHYASCAPCTGYTDGRKRRREAKQARKVREKLQLEDPDSYHHPGPTGTNPYWGEEIAMGPGPPPRRARRTNTGSTRAITTAGTQSSAVSKGGSSLDVARTDGMRLSDDTLEDDNWNRKRYQREDEELWGHDDPSTPLQGTVSGSSGGFHLGRPGTSRSGNYSARAPPVNELHPPVVSLPSPDPADNRWMLQPPPKASVMAGKERASNRSRSGSGASSRVELSLQRQISTKQVQQRLERGETPELPSVSPMSSYSNLVSGQRHDRGRTPQARPPSASSSRRKRRTETALMRTDTNMTRSDTTSTQRSSGESSDTVVKGSPVPRVAPIPDIKIERARQNRPHLSTIVSSGSGVNDFSSSSSPDRLTSSDENAVPEKPSPVHHRLTSTQSSDSTPYLMKHRPPLSTSDISSLNVLQDLVSPHALLNSRFVSAPLVEAKIRLPPSAEDLKRTNSAWSGSRFGISRGWGTEPTTDEQQHIRVPFDNPGPPDRDPRLRWSVDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL08</accession>
    <name>A0A9W9CL08_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006741</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL08"/>
    <dbReference type="OrthoDB" id="2414723at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045068">
        <property type="entry name" value="BACURD1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145:SF8">
        <property type="entry name" value="RE57120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="71"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="283" mass="32978" checksum="2F5D6DFB81F9FCDE" modified="2023-11-08" version="1">MPSEKKATETAMAETQEIRNLLRGLSFQDNLRPAVVESESETVQEEGLAPTGKESQPAPRTPTNPTDYYPKIMTLNIGGRKFTVERETLRNESGLFRLQLSNQYTWSPEADKSYFMEADPDLFAHLLRFMRRPAVFPLFYNSATGFDYDLYNRLEKEAEYFQIGVLHTWIKEKKYLQAVKVHTGNPRIRDLNMVHPIMLPANRSDTWYVIQHTRKVYLCPRQIAIHRGHPDMCGAACHRAQAMNEVVYEDEPFFQVVSVEKEIMFDERVCMVDYTKPRSEEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL50</accession>
    <name>A0A9W9CL50_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AA9 family lytic polysaccharide monooxygenase</fullName>
            <ecNumber evidence="10">1.14.99.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Endo-beta-1,4-glucanase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Glycosyl hydrolase 61 family protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_005870</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Lytic polysaccharide monooxygenase (LMPO) that depolymerizes crystalline and amorphous polysaccharides via the oxidation of scissile alpha- or beta-(1-4)-glycosidic bonds, yielding C1 and/or C4 oxidation products. Catalysis by LPMOs requires the reduction of the active-site copper from Cu(II) to Cu(I) by a reducing agent and H(2)O(2) or O(2) as a cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;4)-beta-D-glucosyl]n+m + reduced acceptor + O2 = 4-dehydro-beta-D-glucosyl-[(1-&gt;4)-beta-D-glucosyl]n-1 + [(1-&gt;4)-beta-D-glucosyl]m + acceptor + H2O.</text>
            <dbReference type="EC" id="1.14.99.56"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">Has a modular structure: an endo-beta-1,4-glucanase catalytic module at the N-terminus, a linker rich in serines and threonines, and a C-terminal carbohydrate-binding module (CBM).</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.56" evidence="10"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL50"/>
    <dbReference type="OrthoDB" id="4849160at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030248">
        <property type="term" value="F:cellulose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21175">
        <property type="entry name" value="LPMO_AA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049892">
        <property type="entry name" value="AA9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005103">
        <property type="entry name" value="AA9_LPMO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000254">
        <property type="entry name" value="CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035971">
        <property type="entry name" value="CBD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33353:SF1">
        <property type="entry name" value="ENDO-BETA-1,4-GLUCANASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33353">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12560)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03443">
        <property type="entry name" value="AA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00734">
        <property type="entry name" value="CBM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00236">
        <property type="entry name" value="fCBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57180">
        <property type="entry name" value="Cellulose-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00562">
        <property type="entry name" value="CBM1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51164">
        <property type="entry name" value="CBM1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="7" id="KW-0186">Copper</keyword>
    <keyword evidence="9 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040761148" description="AA9 family lytic polysaccharide monooxygenase" evidence="11">
        <location>
            <begin position="18"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="CBM1" evidence="12">
        <location>
            <begin position="350"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4368788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="388" mass="39064" checksum="13F63D721A9962D6" modified="2023-11-08" version="1">MFSKALLTTALISAVSAHQNWHQLWVNDVTPGYQVGIRMPPSNSPVTDVTSSDIACNVPSTTGAVVETVEASEGDTIKVQWDSSSHPGPITHMLMAVDDASTATGVGAGWFKIDELDYVDGQWANAIMEAVNMTHEFKLPTGLASGQYLLRSEMLALHGAQTVGGAQFYIGCAQVKITGTGSAGSCTPTIELPGAYNAEDANIYIPNVYNGFDPTNYTAPGGPVAACGAGAGSGSSSPSSTALPAAPANSTVAAVSSSVVAPVTTATAAPIASSVVEAITSSSVVAVAETSVLAPSISIPTANATSIPTSTPLLSLLPSTFATLVKPTPQFPSSTGGGVAAPTGTGTGSGAVKLYYQCGGIGWTGATACVSGASCVKQNEYYSQCLAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM33</accession>
    <name>A0A9W9CM33_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cross-pathway control WD-repeat protein cpc2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cpc2</name>
        <name evidence="5" type="ORF">N0V83_004330</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat G protein beta family. Ribosomal protein RACK1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM33"/>
    <dbReference type="OrthoDB" id="7875889at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045182">
        <property type="term" value="F:translation regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000039">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit beta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045223">
        <property type="entry name" value="RACK1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19868">
        <property type="entry name" value="RECEPTOR FOR ACTIVATED PROTEIN KINASE C RACK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="11"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="59"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="101"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="144"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="188"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4371114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="316" mass="35058" checksum="1245EA8BB1C6BE1A" modified="2023-11-08" version="1">MAEQLVLRGTLEGHSGWVTSLATSLENPNMLLSGSRDKTLIIWNLTRDETSYGYPKRSLHGHSHIVSDCVISSDGAYALSSSWDKTLRLWELSTGATTRRFVGHTNDVLSVSFSADNRQIVSGSRDRTIKLWNTLGDCKYTITDKGHTEWVSCVRFSPNPQNPVIVSAGWDKLVKVWELASCRIQTDHIGHTGYINTVTISPDGSLCASGGKDGTTMLWDLNESKHLYSLSAGDEIHALVFSPNRYWLCAATSSSIIIFDLEKKSKVDELKPEYMEVGKKSREPECVSLAWSADGQTLFAGYTDNRIRCWGVMARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM46</accession>
    <name>A0A9W9CM46_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006342</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM46"/>
    <dbReference type="OrthoDB" id="1896086at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4369257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="214" mass="23387" checksum="7A3B4903023F975B" modified="2023-11-08" version="1" fragment="single">TPAGTRYTVEAIDPTNTAETEAQLKSLYGDNNVIIDGHRGESASWTITSDGDDLTGTIEALESVRVVQPKDAPQTGTGEGRRRLSRRNNGIYTVFAKKPNGSTDTKATEEFLRSKIQPGTNIYHFSNENGDITAWWGLRLDPDAKAAVENHEGVGKVSDDPGTNYARALPPSDEKYILSSLAQTDKRTLLDARSGKWEKQEAADDALVMVSQFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMG1</accession>
    <name>A0A9W9CMG1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C6 finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004389</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMG1"/>
    <dbReference type="OrthoDB" id="5420905at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052973">
        <property type="entry name" value="Fungal_sec-metab_reg_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35392:SF1">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35392">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="45"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="341"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="72"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="361"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="407"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="829" mass="92352" checksum="17C8C3A377A2F656" modified="2023-11-08" version="1">MENQNIDFDGTLYGGVEGGFDPTFDPNRQTYAFSHQTWPLPISAGTMSQQQQHQQHDANQQQQQQQQHQRRINTVSGTAAHNSMPTTTTIAPLQTVGIGFPAAMQPMNPMLATWMNINTDAFAQDSYGQAGMGMSGFDTTYGGSLQTSPVDFGINASQAMMTSMTSMAMQPFGLMTTAGMDLPSTMNSAPFQMADFQQEFTNISNAPDFTAASNAATANSVSTGSPGEQWMEIRSLSSSDNGWVDVGHRNSYDFSDNSAIIFNPAQSLHIRTNSDSSNSNNSDVPPSAHSFSSFEEIHFPMNSPQSEVENNLELVHSHAHSDNCNHTHGLPIEHDFANYISPNQSVSPPMARAEPIQIKQSASSTASPTSSAVSSPPARRRKSPVVPTPTGAKPAKTIVKKTPAHTTKKDATGEKRVGRRRGPLRPDQRQQAHEIRKLRACLRCKFLKKTCDKGDPCAGCQPSHARLWQVPCTRIDIKDIAYFMKDWKADYERHVSLGFSIGNIKGFSSQERMIYVTHGYGHYLPIMAREVFVRDEKCFGVDWFETLNGLHFEINTAKLSAGMEGISRAMLSDYLDRHIDGGFEQFVDEYFEGTLFVTELLKTAYRFYCREKLPVIRKALKLVLAYNLTLHVTMVEGLGEEEHPLGKVDDQQSKFCGKTVAPVMINFQVKCALADMWRELQKDVLEELSSLYQSVYSGDKLKNWPTIFMLAAILLAVWELIQFDCNYRVPDVGAVNKFCSEMEGTPVGVIVGLFSAISQKLPSFTEWDSRKHHHLLNSNPAVCDAMTEVRAHVTKYDTYLRSRSDCKFDRSDFDSLSNKFLSKLVIRAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMI1</accession>
    <name>A0A9W9CMI1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004768</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMI1"/>
    <dbReference type="OrthoDB" id="2126185at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="336" mass="38703" checksum="FC8EEB933D7D4ABC" modified="2023-11-08" version="1">MAPPGFSPPGYSIPQRYQHREAPPAGVDTNTRPYFILFSYLIEYFKISYKTWLMWRSYYELDPHLRHWGLWLKESSLYKEAWETVIVGNARYWWSHQIFFFALGLGLYLEQKGIRRNIKYTWAFMLLGQIVAISFATNLFFLTLLLSPPAPPPPSSSGYYRSKWLGPWLLNLVAILATAYPALLLADEHYWHHPTAFMPVLLAPHIALLALPFARTIIPARYFNDNDVAFTDKVYNWMWALVLGNAGFIFLRATYTSYMYSGFAGISNALLEHPAVSFVGFDVIFCWVTWICWYRTQIKGVRHAVKDYSAGTHDELNDDSSTAVGTSGYDGGVRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMW8</accession>
    <name>A0A9W9CMW8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003734</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4371961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMW8"/>
    <dbReference type="OrthoDB" id="5069333at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4371961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="266" mass="29360" checksum="391534F615B1DF4C" modified="2023-11-08" version="1">MAPGHYTPPAMEYANPKPWDLGPRYGLQNNDTDMATFWALNADGDCTCHAGVTEMLASMRSASSNNNQPMSLDAQLAKLKRCIVSSEMSMGCAHRREDSEPIHIMAVAMLIGYVIDDFKMLASESSLRRCSPPFSSSAAAALDMATLGTTDSGSSSPGGSTNMSLGGLWEPRLSWGVLQLEEDDEMDLRQRLYMLSFRKLERLLSQLTLYLRDLHNTGASLQDPSRHMAFVIACDYTRLWLEKKAGEVKMLFSVPSREETMDSGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN08</accession>
    <name>A0A9W9CN08_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cep57 centrosome microtubule-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_004542</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN08"/>
    <dbReference type="OrthoDB" id="76453at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051756">
        <property type="entry name" value="Centrosomal_MT-associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024957">
        <property type="entry name" value="Cep57_MT-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025925">
        <property type="entry name" value="PPC89_CLD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19336:SF9">
        <property type="entry name" value="SPINDLE POLE BODY PROTEIN PPC89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19336">
        <property type="entry name" value="UNCHARACTERIZED DUF1167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14197">
        <property type="entry name" value="Cep57_CLD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06657">
        <property type="entry name" value="Cep57_MT_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPC89 centrosome localisation" evidence="7">
        <location>
            <begin position="405"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Cep57 centrosome microtubule-binding" evidence="6">
        <location>
            <begin position="1006"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="128"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="377"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="669"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="830"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1141"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="341"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="402"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="144"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="169"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="186"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="231"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="675"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="704"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="843"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1145"/>
            <end position="1163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4371325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1180" mass="133558" checksum="B09AF8819EB174DD" modified="2023-11-08" version="1">MQSSPPTSDGKARAIRELSRSLSHSPRNIASPSPPPSDSNPTKNSGFGSESDDFSNNPDVFMSTQHRLEDDTNTLPQYPRIRSTAKKVNTWHMLRSEQPVPDTSMVNKEFGDFDHSLSDEEAMSIEQARGLNRSNRGTPDKIRSQFNSLYDITPPTNRSRKSYAAETGSLRRDAQIRRASRNDFESASPRPASARHAPANQDRRHTSLAQLHAKISEDESSFMDQRPPTLTVDSTKNTRWGNRSRQTSLQIDGVVDDSRRVNATPKSRPATAQNATAQSFILPDLPNLTELVSGVFDDGTPVFSKNAPARSRFSAPPGRPQHIPVEGVPIPDEEKAIFAALHQLQDKVAQMEAERAEQERKLEEQDMELIELRATTQAQEKLRRSDSAHDSDGSKSSWKVEKTRLDATVQTLRTKLDRADRKLAVLEIEKKRLNTERDNMAGQLGVAFQTCEELKNEKGALSTENDTLRQEIDNLRAENEELRDQLAHDQSQHREETVQLRQQFDQAANATEKENATLHAELARVRAQHDENTQQLSRKDIELRKARQEQAEYAQLKSYHESLQSQLANLKAKREEEVKRWTRQEASLKAQVGRRDETIRQFQDMTQEQTNEAMRLDNDNLRNELAQLSAKLEDETEQWIRKEAELKRKVQRREDAARKTLDMTREVLSAREANEQQFDASGPTSKGKGKENAFAEPLQRKPSYRREDTRTRIHNRVQQEVRNSKAVGAQQSSQFGESPRKSYAGVSRFSNRSVSAPIPADKHARVESDVESTTDLSLAPRGTPYTSRSIRSVKTTTAIQPPADLDLTELSLIDSNQIAQLRRALEEERAAARRRASAPLDQQARDDTLRSQRQTREDTVRSIASAKSERRPSLPRKSSLKDITQRTNVTQFEEDVTGDVSNLDADVEATQTKQSAIDASMLSNTSRRRRSAPTEMTSAFIVPDIKLDTHKQTTTTVNISQKTTSKDHDNTNCTVCRRDGLTTATDAFRVPKLVPVSSRMPDDVDATLRPARSPKEALALVVKELQDERAHMHMELAVMRAMLESHDASLGNRKRREINASMQEILRRLEIKDMQIYNLYDVLEGQQTDDLTELDVENLTQQIRAEEEKTQSEPEKKAKKVTIRSFVDEEDDNSIEVGRGVSHELEDEETQELPWEGFEDTGMEGDVSYGALQGWRTSVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN65</accession>
    <name>A0A9W9CN65_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004454</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN65"/>
    <dbReference type="OrthoDB" id="15893at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039196">
        <property type="entry name" value="Fmc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28015">
        <property type="entry name" value="ATP SYNTHASE ASSEMBLY FACTOR FMC1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28015:SF1">
        <property type="entry name" value="ATP SYNTHASE ASSEMBLY FACTOR FMC1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="114" mass="13022" checksum="AAE8ED9924E01B92" modified="2023-11-08" version="1">MSQAPKIRSLYRRLLRELPSTPQTTRTQHQKLSAPSHLQQRIRSTLSSSSANTSGQIQQAEQFIQYVQAQRTYATLLERYNPGMGMSEEERVRLSARRVGMNLPVEFEGSGEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNA7</accession>
    <name>A0A9W9CNA7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004559</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNA7"/>
    <dbReference type="OrthoDB" id="47172at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF101">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="1">
        <location>
            <begin position="310"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="483" mass="54710" checksum="C1558F2FA979AFC3" modified="2023-11-08" version="1">MNSDAPTLQEIIQLTRQELTTAQKSDNIRECGRAALSLLPSDPQLCSELAYQSLHSVPYKQVKTCWRRLYTDASLWKVVRLAEEPALPQSTKKDSNVEEDEFKRTWIDEVVRVLDMALILTGAPGREEIVELWFSALKASLPSLHHTLDELDLDGPERPVKRQKLSSSTLTSHIPSTFITGLSGPALKLRHPVPRRNELGLSTFQNIVGNTNTHTPLIIEGAIRHWPALDERPWNFPGYLLEQTLGGRRLVPVEVGKSYTDEGWGQKIITFKNFMETYMLESRKKEEKDGQKDHTDHATIAERGPVQQVGYLAQHDLFAQVPSLRSDISIPDYCYCDPAPSPHLTHVKPVPKLDEPLLNAWFGPAGTVSPLHTDPYHNILAQVVGYKYVRLYAPEETPKLYPRGVDENGVDMSNTSLVDLDEAMIVYHEISCWKTSGGEDAENRDDDIHMKSEEIEQRRRVFEEQHPGFRNARMVALCQKSHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP95</accession>
    <name>A0A9W9CP95_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glutathione S-transferase 5</fullName>
            <ecNumber evidence="5">2.5.1.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">gst5_1</name>
        <name evidence="5" type="ORF">N0V83_003402</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP95"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03056">
        <property type="entry name" value="GST_N_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF2">
        <property type="entry name" value="HYPOTHETICAL GLUTATHIONE S-TRANSFERASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="4">
        <location>
            <begin position="86"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4373111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="213" mass="24325" checksum="D26CBE8FDEE70161" modified="2023-11-08" version="1">MHLYDSTVPSGNAYKVQLLLTQLNIAYKQTSLDILATPSESRKPEFLAINPNGHVPALVLDDGSILTESNAILFYLAEGSSYLPDDKFKRAQVLQWMFFEQDSHKPYVSKWKYRTYWAPHGFDDLPKDQVDDMRVKGQTAIDVMEKHLSDSPGRDWFVGDSYSIADIALYAYTSHAEILGFRVGQNVKRWLARVKETEGFVRIKKDPTGKNPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPF2</accession>
    <name>A0A9W9CPF2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Centromere protein X</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_003584</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CENP-X/MHF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPF2"/>
    <dbReference type="OrthoDB" id="2500381at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071821">
        <property type="term" value="C:FANCM-MHF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22921">
        <property type="entry name" value="HFD_CENP-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000139">
        <property type="entry name" value="AaceriACL158Wp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018552">
        <property type="entry name" value="CENP-X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28680">
        <property type="entry name" value="CENTROMERE PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28680:SF1">
        <property type="entry name" value="CENTROMERE PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09415">
        <property type="entry name" value="CENP-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="40"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4373290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="20425" checksum="2CEAEFE46273DCBB" modified="2023-11-08" version="1">MKQITMPPKNGTANASRRKGPQFNPPRPVKAPPQTSTAQRAPATASRTAGAAKKANAPARQPAATIISSSEDEREEEEQYEDDSDDLMEDATTRDHGRPATSSAPVAPPIPAPLLARLLYENFEDSNTQIQKGAMNLVGKYMEIFVREAFARAQHERENAVKRGGISDGFLQVEDLEKLAPQLVLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPZ5</accession>
    <name>A0A9W9CPZ5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Iron-sulfur clusters transporter ATM1, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Iron-sulfur clusters transporter atm1, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ATM1</name>
        <name evidence="17" type="ORF">N0V83_002361</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPZ5"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140466">
        <property type="term" value="P:iron-sulfur cluster export from the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18582">
        <property type="entry name" value="ABC_6TM_ATM1_ABCB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03253">
        <property type="entry name" value="ABCC_ATM1_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000004">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000186">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF402">
        <property type="entry name" value="IRON-SULFUR CLUSTERS TRANSPORTER ABCB7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="230"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="118"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="440"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="680"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="685"/>
            <end position="700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4375275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="706" mass="78196" checksum="1EBE2776B12184DB" modified="2023-11-08" version="1">MLIPPLARAARRPGLRAHSLSLRPTIFHATRVFTSSPLHRKDVHGQRTAPGSKVALEETKAAKPVNDPAKAKVAKDPLLQEQVLTNKEQRKADWAIMKEMSQYLWPKNNLGTRFRVGLSVGLLVGAKLLNVQVPFYFKNIVDSMNIDFATMGGTAGTVAGSMIVAYGLTRIGATISQELRNAVFASVAQKAIRKVACSVYEHLLRLDLSFHLSRQTGGLTRAIDRGTKGISFLLTSMVFHIVPTALEISLVCGILTYQYGWQFAAITGMTMTAYSVFTIMTTSWRTKFRKQANAADNKAATVAVDSMINYEAVKYFNNENYEVGRYDKALKDYEKASIKVATSLAFLNSGQNIIFSSALTAMMYLACNGVATGQLTVGDLVMVNQLVFQLSVPLNFLGSMYRELRQSLLDMETLFNLQKVNVQVKDAPNAKPLALTKGEIKFENVSFGYRPDRPIIKNLNLTIPAGKKIAIVGPSGCGKSTILRLLFRYYDAQQGRILIDGQDIRDVSLDSLRRAIGVVPQDTPLFNNTIQHNIHYGNLDATQEQVHEAARRAHIHESIVKFPDGYETMVGERGMMISGGEKQRLAVARLILKDPPFLFFDEATSALDTHTEQALLSHINSLVKEKRRTSVFVAHRLRTIYDSDVIIVLREGNVAESGSHQQLIDSGGLYSELWSAQETMFAEPKEEEDEGVALDDDVEGGEGKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ08</accession>
    <name>A0A9W9CQ08_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002963</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ08"/>
    <dbReference type="OrthoDB" id="2898509at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052228">
        <property type="entry name" value="Sec_Metab_Biosynth_Oxidored"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47534:SF2">
        <property type="entry name" value="KETOREDUCTASE (KR) DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47534">
        <property type="entry name" value="YALI0E05731P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="166" mass="18067" checksum="6F733F4DAA5A76FC" modified="2023-11-08" version="1">MVSLSEVRAHNASLKSLPAGLVAVFVGGTSGIGLFTARELVRNTTSPTIYLIGRNATEASKIIEELRTINTSSQVSFIQKDVSLLKNVDEACREIKTKEKQVNLLFMTCGYLTLKGRDETAEGLDRKFALHYYTRMRFTNGEEDAGGGEGEGGLAAYGGGVWEDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQK1</accession>
    <name>A0A9W9CQK1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_001609</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQK1"/>
    <dbReference type="OrthoDB" id="413885at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09630">
        <property type="entry name" value="CDH_like_cytochrome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1210">
        <property type="entry name" value="Cellobiose dehydrogenase, cytochrome domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.410.10">
        <property type="entry name" value="Cholesterol Oxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015920">
        <property type="entry name" value="Cellobiose_DH-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053208">
        <property type="entry name" value="GMC_Oxidoreductase_CD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47190">
        <property type="entry name" value="DEHYDROGENASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47190:SF4">
        <property type="entry name" value="DEHYDROGENASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16010">
        <property type="entry name" value="CDH-cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49344">
        <property type="entry name" value="CBD9-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00623">
        <property type="entry name" value="GMC_OXRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040798125" description="Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein" evidence="4">
        <location>
            <begin position="23"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="5">
        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="6">
        <location>
            <begin position="487"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="206"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4376326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="771" mass="80542" checksum="4752FCBEE5155E5D" modified="2023-11-08" version="1">MSASRIFTSSLLLAASLAGVQAQSTSPYLDEATGITFQQYVSTAGLSFGIALPEGAGTGFIGQLSATTTGWAAVTLSGSMRNSLLVVSWPNGDEVVSSLRTTTGYSNPGVFTGNATIKPIAAGTSVNDTGYTYTFLCESCIIDDMTFDATADSGILGWALGTNPPDTPSSASSVLQYHDGGFGIYGFDLAGAKSANFSSWAALASSGNSTTTPPPSSGGGNSSTPAIPNNGTVSVSNSTYDYIVVGGGASGLVVSERIAETGKSVLVLERGGPSFFSSGGDLVVPWNDTLTAYEVPGLFNALSSFPGNNAYCSDTPEIAGCILGGGTAVNGLQFVRPPSFDFDDNWPQGWKWAEVEASAARFYERNPGSTSPSTDGKYYDNAVWDVSSKFFAGAGWKQVDTNEQPDEKFQVYSYPAINTMGGLRAGPVRTYLPLVADLPNFKLQLHTKVIRTLRTNSTVTGVEVELEDGARQIININQGGKVILAAGAMSSPRILFNSGIGPADQINIVKSGNTGVTLPPESQWINLPVGFVRDHALISVNFNVTAGMTIMGADEFTSPSNMTKSLYAHGAGPLAQSFNRAMSYTTITNDEGHKTVIQNHIYSRANNTISFLFATTHGVTSTGMLGITPDGNTEWTVNPYLTSESDKAAMAAALDDLLAISRRPNSTLSYIGPSNATGASIIASQAPAAGTHMTGTTIMGTDDGTKNGSSVVDPDCKVYGTDNLFVVDAGMHPDVPTGNTQAIVMVAAEHAVQKILALDGSTASNTTYRWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQQ5</accession>
    <name>A0A9W9CQQ5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SRR1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001761</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQQ5"/>
    <dbReference type="OrthoDB" id="5230585at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012942">
        <property type="entry name" value="SRR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42080">
        <property type="entry name" value="SRR1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42080:SF1">
        <property type="entry name" value="SRR1-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07985">
        <property type="entry name" value="SRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRR1-like" evidence="2">
        <location>
            <begin position="162"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4376477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="369" mass="42271" checksum="A2246F67EEB6424E" modified="2023-11-08" version="1">MASEQQPPSATKIDDSNGVMHFPIGKSRLEREALLKYRLEECEPVYTHKRFREWAEYIFYINGKGFTQEVQTFDAQGNNKKILVRAGGPPKQVMLPSYHQWAVLDFIQPCSSPGFQNMLFRLFDAPERQQPEQCCKYVKEENLKQLWVGWQQSPFCKQVIEHITKNAYNMAVVENFVCFGLGPIEESDDPEDHIQRTFLQHAMAVEIRDALVRTQRKEDLLFQIPIAVQDPRYCKNCVRLLLDVLKVGVMNTPTGFPTVDKTTFVISKAPSVCVRQIVADLTADAGGPAGMLCDPIEDNGLDEMAHNQHSDPSSPRLWDFKERNKELELMLDDTEDKIKYMTDDVPPVEVDGLPNRVFDDVGLYLRKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQR8</accession>
    <name>A0A9W9CQR8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="12">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">N0V83_002408</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:21249"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="12"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10248617at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000011">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.150:FF:000004">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000008">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000016">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1110.10:FF:000007">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.16.10">
        <property type="entry name" value="Hedgehog/Intein (Hint) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009674">
        <property type="entry name" value="Rpa2_dom_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06883">
        <property type="entry name" value="RNA_pol_Rpa2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="Hedgehog/intein (Hint) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="16">
        <location>
            <begin position="44"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="15">
        <location>
            <begin position="208"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="17">
        <location>
            <begin position="495"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase I subunit RPA2" evidence="18">
        <location>
            <begin position="600"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="13">
        <location>
            <begin position="1197"/>
            <end position="1569"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="14">
        <location>
            <begin position="1571"/>
            <end position="1616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF06883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ4375322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1707" mass="190523" checksum="24BCA63E0CD5A856" modified="2023-11-08" version="1">MAPAAAVSRKPQQPWTTNFDTQRRQKLFQSAPKDTTAYPTLAEAVSPHINSFNAIFAAGGQLEQGIKDIGTKVFLDGDPFARPEDIGRRNRLSVRIQDVFLDRAVLPPSNKVALKNRNIFPAECRERHATYRGKMRARLEYRVNNGDWREEVCDLGTVPIMLRSNRCHLENLSPDQLIKAKEESEELGGYFIVNGIEKIIRMLQVNRRNYPMAIKRGAFTNRGPGYTPYGILIRSMRPDQTSQTNAIHYLNDGNVNLRFSWRKAEYLVPVMMVLKALVETNDREIFEGLIGAAGSEGLAEKQFVTDRVELLLRTYKVYGLHSKAKTRAYLGQKFKVVLQIPEDTSDEDAGTEFLRRIVLPHLGAYSVTPAQDADKFRMLLFMIRKLYALVEGECAVDNPDAVQNQEILLGGQLYGMILKERLEDWLLSIRVGVQEWGRRAEWKPFTSEEFKKDFVPKILRRSQLGIGAAMEYFLSTGNLISPTGLDLQQTAGFVVVAEKINFYRFISHFRMVHRGAFFAQLKTTTVRKLLPESWGFMCPVHTPDGSPCGLLNHFAHKCKLATHNVDVSAIPRLVAQLGVSSKSAASLDASVVVQLDGRVLGFCSPKQAKVIADTLRYWKVEGSHSVPLELEIGYVPNSNGGQYPGIYMFSQISRMYRPVKYLPLNKLDYVGPFEQPYMSISCTDAEIVSGDSTHVEYDPTNIFSIIANMTPFSDFNQSPRNMYQCLSADHEVLTNAGWKALPAIQEADQVMTMDLQTGVQQWNPVQATTRLARSGSLYRLKSGGMDAVCNESHRWYLNTKDRPTTYTAYTTQQMLDNQLVALGNSSGKATSQWHRKSGTTTHINHKIPTVGHNGNAMYLWPDCSWLPREFVTDEACNLDWCRFIGLVMGDGGINHVTNAQGQECHYVQIHQCTGKPAAKAYVEDLLERLAAKLPDFVVNPPCVSNIEEKHTWTINHRTMYHFFLPMIRGPQSYDPLDDAMCRSYEAPRYQALSRSDNGPILPVPAGWKTGNWWNLRRWMNYTWLYLLARDQARAMIKGMAAADGEWSLKFKAQPNTGGRGRGADSAKRIKLSMESVQSEQLAVPPIKRGVVRVFNSSIPLMHDMSVLGLLADTRVNIGVNHKKGEWMPSIGSDANATEWRISFSFGDGELAVAAPKPEPYNNPRHDGFVYCLTVPNGNFLARRTVEYSDNDKSQEVLDAAQKPFYTGNCQMGKQSMGTPGTALRYRTDNKTYRLQTGQTPIVRPPLHNEYGLDNFPNGMNAVVAVISYTGYDMDDAMILNKSAHERGFGHGTIYKTKICDLEEGSRKNRSGRTVSKLFGFAPDGLITAAKKETLDEDGFPRIGTMLRSGDVIAAWHTVSYDPATDDYVNRDAQTQFFKYKEDELAFVEEVRIIGAEDGTQPCQKISIKLRVPRSPVIGDKFSSRHGQKGVASQKWPSIDMPFSESGIQPDVIINPHAFPSRMTIGMFVESLAGKAGALHGIAQDSTPFRFDERNTAVDFFGHQLMKAGYNYYGNEPMYSGITGQELAADIYVGVVYYQRLRHMVNDKYQVRTTGPINSTTGQPIKGRKKGGGIRVGEMERDALIAHGTAFLLQDRLMNCSDYTKAAICRACGSFLSTAPTVSEFGRKKDKAGAAGATGGGNAATIRCRRCAKLADGKTGKADVWMDGQGMRFSGGDDIAVVAVPGVLKYLDVELASMGVRLKFKVDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR31</accession>
    <name>A0A9W9CR31_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_001264</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR31"/>
    <dbReference type="OrthoDB" id="21018at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072354">
        <property type="term" value="F:histone H3T3 kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024604">
        <property type="entry name" value="GSG2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24419">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR-ASSOCIATED KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24419:SF18">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE HASPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12330">
        <property type="entry name" value="Haspin_kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01331">
        <property type="entry name" value="DUF3635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine/threonine-protein kinase haspin C-terminal" evidence="10">
        <location>
            <begin position="536"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="69"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="131"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="191"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="365"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="565"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="196"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="378"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4375984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="734" mass="82478" checksum="CF7324F94C1041C1" modified="2023-11-08" version="1">MWIVYGKKKATARQQISFTKFLSPEKVGVLENADSAVQDDWQQQLKDAARDVSEGIRGEDDVALLEEGLGSLQIEPTQTADDEGLKKKKSKSRRGQQNSKKSEEQEDTVSVDDALEVGMKALSIECPVQEGAVERKKPTRTRKALADRSPNAGGEANCKTRTTKKQPKRAILECVEIKTKSDVPVCTQKRQEKLSSKTTLPQQNVQLPTPEPTPEPDDIYSVYASQLLLLSDRRRVVTFDDWSAELEPHFEITKIAEASFSEVYRLSATSTTHGIREESVLKVVALKTPPDVPLPSQLQTRTVRDRDGQLAKEMTEREEKDQWKSDVADVLSEVKLLQNLNHIPGFTVVRDMTIVQGRPSSSFNDAWKNWNKSRPRGKKSEFPDPSKKTSYDDTQLWAVVEMQDAGTDCEKMMELGGLSSIWEVWDVFWSVCLSIAKAEEACRFEHRDLHLGNICVRSSRPTGDALQPNIKDPLRRKLRFTGLETTVIDYTLSRADIVSAPSRRSSILSSVSNSTASSSLESPAMDVAYLDLDKDPALFEGDASQEYQYEIYRYMRGAALYNKPLQSTPTVSPEVPATPRRSPRKNTHIRFDEDEDDAPRRSPRKTAAPQVDDAIVTNTTTKAEKDVWRCFHPKTNLVWAHFLLHKLLNHLQSYKATPDKLSTKEIMQNILVDGEAADELKIKKKAVGLYKVLQKVSERLCPVALGREDGLSSVKELVVLALEERWLRVGDVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR49</accession>
    <name>A0A9W9CR49_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3-phytase</fullName>
            <ecNumber evidence="2">3.1.3.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_000396</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histidine acid phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.8" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR49"/>
    <dbReference type="OrthoDB" id="6509975at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016158">
        <property type="term" value="F:inositol hexakisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000033">
        <property type="entry name" value="Chromosome 12, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963:SF55">
        <property type="entry name" value="PHOSPHATASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00616">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040717989" description="3-phytase" evidence="5">
        <location>
            <begin position="23"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4377571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="555" mass="59915" checksum="5843C7B62FDF18E7" modified="2023-11-08" version="1">MKMQLTPARAAAILAFVAQAGAAPTTIQAASNAGASTADPYPPSGTAVNSALFPPESKVGFPGPTPTGAEPAAIQTAKSYPYNDGNANQFPLVNPQPHGSDAERSTFDISKYWGNLSPWYSLRSADYGLPDANPLAPEGCDVTQVHLLYRHGARYPTSGAAPATFAQKVMNATKTGLSVSGDLSFLADWTYKLGAELLTPFGRSQNFLLGIEYRQLYGHLLNNFTESKTIPVFRTESQDRMVKTAENFAAGFFGVPEYLDEVNIEILVETLGVNNSGAPYEICNNSNIATKGSIGSTVANKFSVNAFNSTIARLQSQASGINFTSTDAIAMLQLCSYETHALGYSAFCDLFSEEDYLNYEYYFDLSFYYNNGPGSPVAAAQGKGYLDEFIARFTGKYPSAQSALNETFDNTTTFFPLNQSIYADATHEVVVLDTLTAFNLTALFDGPPLDAAGNRRQNSFVASKLVPFATHFTTQVLSCPAMQPTKQIRFIVNDAVIPLHNSHSGCPNDPNGLCSFDNVVSVLQKRSAEINFNYDCFANYTASAGHDYNGRAPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSF4</accession>
    <name>A0A9W9CSF4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">feruloyl esterase</fullName>
            <ecNumber evidence="2">3.1.1.73</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_000882</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>feruloyl-polysaccharide + H2O = ferulate + polysaccharide.</text>
            <dbReference type="EC" id="3.1.1.73"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.73" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSF4"/>
    <dbReference type="OrthoDB" id="424610at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030600">
        <property type="term" value="F:feruloyl esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043595">
        <property type="entry name" value="FaeB/C/D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38050:SF2">
        <property type="entry name" value="FERULOYL ESTERASE C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0858">Xylan degradation</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040788193" description="feruloyl esterase" evidence="10">
        <location>
            <begin position="21"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4378052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="469" mass="49666" checksum="BED0B1498FCF0787" modified="2023-11-08" version="1">MSPQSAVTLLLASILHLTLALPQSPLPFLTPRASSSGCGKSVFLPGVTQYRFGLKSSGQDRSYSYHLPSSYDSNKAYPIVLGFHGSSSIGAFFELDTKMSEARYSGEKIMVYPNGESGSWAGPTYHSGSSVAEDVQFVKDVIEDVKGRFCVDEEKVFGVGYVLEWPFTLLRTCITDENSMSNGGGFIGTIACDSVGSALFTALAAHSGAFYTDVNGPANGCAPSKVLPILEIHGAADKTVNYEGGQGDGGPEPPISSWLEWWSQRNNCSNKTEEELNDGKVHHYSWTCGGKTGLLQHYKVDDLGIKDTRTRIERRTSISPYSYILYSSLLASATANSDPVSFIIILQSLINKFEISISDIKMRFTIAAFVAFAAVATAVLTPNDAGAKNVGAGNGAQFITGGCVSDADCSSACCSQVAATGDGVCSAEAASNQNGKTGCGFNDPNAAAVIAAAQAQVQKQGFKRAIRDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXF1</accession>
    <name>A0A9W8XXF1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nephrocystin 3-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_010899</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXF1"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF17">
        <property type="entry name" value="FUNGAL STAND N-TERMINAL GOODBYE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="3">
        <location>
            <begin position="232"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="689"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4361958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1176" mass="132628" checksum="6A3CDF8932C74A67" modified="2023-11-08" version="1">MAIVPDTMKAEESLPSGESVTICQQHGEDLWKLAFEKAGFDEHQRGVLLGESSGASWSPSVLVERVSDSTIARFRDCKSSKWFSGPRDHTAAVSAQAKTVFTAILALKNFVDAGMKFDITGMIASFSALVEEPLTTLKQDIAELDGSAKEWLDPIAHQFRMQESQRLESGVAELLSQASHATEKFEIMYENLRTADEDRIRDWLCEDRFSHTGRHIQIQNDLRQYKDKLKPGQWLLNSDEFEKWVYYPQQLLWLQGNSGSGKSYLCSIVVEHLRATYDMDLRKIVAFWFFDSLVADTDNLERMLRSLINQLMPGPELIPAFVAAFRKNHHNTQSLPTLEKLITLLLQVVCNLKRDIFLVIDGLDQCGHTESVSLKLKGLLPVIDINKNNGQDLDDFMLFKLSKDIWPKVTSSATKFEVMRALRSDTMKSFLWTEAQIRFLSEFRTDSSIRKALDKVPDTLEALYCKALGVIKEEERDLARSMLHLLCLMRTRIKRDALADYAGLARPADIPAICGNNLVDVNEDGFVQLIQPALQQVLESDNIRQNCSLQSFHLLHDDAHAKITERCLDYISRCTEGPFCHYACEFWHIHFKESGALKDPDNPVFDLVLRFLLSENNAALRFWRKQYVGPKERYQRQKGEGLKGEIPLLHYCPEPGLVSPIYIALELEMPVLARSLMKINGNVSQAYGRLGTALQVAAHRGYGDIVGQLLQLGADPNATPNGPLGTALFAACISGNRDIVRILIQQGPTKCTLDNQYRDVGTALQYASFRGYKGIVDDLLAAGADVLVTGGVLGDALQAAAVGRHISIVKTLLANGAHSTRLHGLFGNALLAACILGDEKTRDLLIADREKRNNAGRLWESAIDRAYHLDEYWKKPYWMDSLLQEGPNCPQIVWGVLLSQQKRAAGIIHYATPKVVSGVMRQHVQKVPRLLHEELHLEVQATFKTDLNGSLLEEPDMDGCASPFFAAKHFFRSAVLALLDKLDKSERHNSLIWACLTKLLATRRRTAAYEDYVLNSHDDMAVYQLQMSLVDLFFNLVHALFVVGYPIFPRRITHAFRVEYNRDLALGKVPSQQELLQIVEKCEEATHTEASRYLLAKQATSINEMQREQRALVSKMEVMANKINKLSSIVRPTEVEDANGNIQQEAQAEQEVLAEQEVQAEQKIAHPNKWLDNDWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXX0</accession>
    <name>A0A9W8XXX0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Pectate lyase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_010777</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the polysaccharide lyase 9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXX0"/>
    <dbReference type="OrthoDB" id="5561043at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016837">
        <property type="term" value="F:carbon-oxygen lyase activity, acting on polysaccharides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011459">
        <property type="entry name" value="DUF1565"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053868">
        <property type="entry name" value="Pel9A-like_beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052052">
        <property type="entry name" value="Polysaccharide_Lyase_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40088">
        <property type="entry name" value="PECTATE LYASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40088:SF1">
        <property type="entry name" value="PECTATE LYASE PEL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07602">
        <property type="entry name" value="DUF1565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22842">
        <property type="entry name" value="Pel9A-like_beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040748135" description="Pectate lyase" evidence="9">
        <location>
            <begin position="19"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1565" evidence="10">
        <location>
            <begin position="26"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Pel9A-like right handed beta-helix region" evidence="11">
        <location>
            <begin position="142"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF22842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4361836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="41964" checksum="A217503ADE58BCBD" modified="2023-11-08" version="1">MRLTTLQTIFLLLPSVLGATIYVAPTGSDTAAGTLAAPFKSIQSAIDKAVAGDTIYLRGGTYSLTNNIQIQKSGTSSAPYILSAYQSEVPVIDGEELPSTPAPVGGSVAAADRGILHIENANYWKIIGLELKNGPYGVYATASNNNVFERLVTHDNYETGFQLQGASANNQILYLDSYMNRDPRKNGESADGLGIKEGSGEGNIVRGARLWNNVDDGLDLWEFKSKITIVDTISWGNGVNRWNFPDFGGDGNGFKLGGGDAADIGPANHNITNCIAFSNAAKGFTDNSQPGNFIMLRNTAYNNPDVGFNVKTAVASLGNNVAARNKGSTKNADQVSLSSAQKLSGNSWETSATWEDSSFKSVDVSLVQGQRGADGKIVASDFLVPASGAGIGATTQWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY49</accession>
    <name>A0A9W8XY49_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_010924</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY49"/>
    <dbReference type="OrthoDB" id="3740062at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4361983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="247" mass="27236" checksum="041A5DF5C0E5F0E5" modified="2023-11-08" version="1">MSDTTTSSKTVLQNTALKENIRLRVQQTVNTINNMRGGENLSNYSISARKELASSETLDVILGPGQDASMADEVPKLALVVASKTFREKIVEKPEIPELKVVSASIDIPSVAILMNWLKDAVHSKAHQIPKVPIPADIVDKAKLVHAANILGMDRYVNHVVASFRHDVRLIIPSPEQCSNLEKYGISSDHAVSQAVGERFGYLLRTGKFFGDRHMLMRFLARSEKIGQAVRDADARAQAKRAAQNQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY58</accession>
    <name>A0A9W8XY58_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GDSL lipase/acylhydrolase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010071</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY58"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01846">
        <property type="entry name" value="fatty_acyltransferase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051058">
        <property type="entry name" value="GDSL_Est/Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648">
        <property type="entry name" value="GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648:SF22">
        <property type="entry name" value="GDSL LIPASE_ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040719950" description="GDSL lipase/acylhydrolase family protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="39423" checksum="6466D83AAACFFF21" modified="2023-11-08" version="1">MVSSWSLRLLVVNLAVAVGHAGRASQGWKDGKIKTIVTFGDSYTDENRLGYFISNNGSAPPAGWEQPVGLNTASGGLSWARYASFYSNSSLYNYAVSGAVCSNEITPRFFSAINAPFPDIEGYELPAFIADSKYISPNGTKFFTGKPDSTVYAIWIGTNDLGNDAFLTDSQVPGKTVKDYIDCVYAQVSRLYDNGARYFVILNLAPLNLLPQYALPEDGGLPAAQYFPEKQGKNISEISGRMQETVAALNSVYAYRTPFESQMTQNFEGAKFADFDVNALMTDIYYHPASYLNGTGEPLNVQGVSHACDVKGQNCTLSKSPDSFMWYDPLHPSEQTDRIVAREFVGVLGGRSKWARYWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYM1</accession>
    <name>A0A9W8XYM1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010496</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYM1"/>
    <dbReference type="OrthoDB" id="1028014at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053185">
        <property type="entry name" value="SET_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332:SF6">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="98"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="391"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="404"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="421" mass="46907" checksum="252FB1FA90964CC7" modified="2023-11-08" version="1">MGAAKRPACFSKDSPIFGIMKRGFGATVVTFLDISLLPNVDGDGLGFHQTFCAYTNADYNNGRGISFVVTPEVAASISSETFGLGVGGLDGQVGSEMGMWEVKETKEMGQGLFAREDVAAIFAGESLIIKTPVLFVAKQLLDTPSTSRRQLVLQKAVEQLPEKTRTAVEKLAESRDGGVKDMILTNGITVKWPWADEVPELLAVTPEVARINHACRPNALWRFNDYTLGFEVFALKDIKPGEEITLSYGFESRSHRRRVKSIEANLGFTCRCPLCSANETLIEQSNDRLSEIKALKSVLPSDQKDSPQLLGLLPNLIKNLEEEDLHTEIPMYEEILAYTWSSFGIEVRAKYWAGRARRHWAVIAGKESWETKRCADLENDVKAHASWMSWDGEDPWEGVGQGHPWDEREGDDHDHDHDHSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYV3</accession>
    <name>A0A9W8XYV3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010821</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYV3"/>
    <dbReference type="OrthoDB" id="19657at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050471">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43433:SF5">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43433">
        <property type="entry name" value="HYDROLASE, ALPHA/BETA FOLD FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="53"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="327" mass="36622" checksum="9DAE0632B9A715B0" modified="2023-11-08" version="1">MAPLTADELVKHPEYNHTIWPLEPEKKGKVQVAKDRGGPIDIAYEVHGHGDRHLVWVMGLGGMMYAWQRQTKDFGHTKGDQYSSLVFDNRGIGDSSKPRFRYSTSEMAKDVVELVDHIGWTGKRELHLIGISMGGMIAQELAMLIPERICTLSLVSTAAGLFRTTGFLENLWNRANLFIPKSLDTQIAAVKANLYTPLWLSSPDTLPAVSSTPFPTNGDRFAANELWKRQHPQYFGKPGFILQAIAAGWHHKSAQQLQEIAEKGVTGRENWLGVEEEEDVWAEGLVEKHFVKGQGHVLPAEMREEFMGWQEGLIERGVRLNAGEEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZE6</accession>
    <name>A0A9W8XZE6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010992</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZE6"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="200" mass="22679" checksum="16FF6720CBAE52C5" modified="2023-11-08" version="1">MSEGKMETSPPPSKFNPEKFEPYKSPFTIYKVKYPINSNTTERTGYTIDKYWHDVCQDFGWPTESNQFETVDQLSRAKSLVVAEYGKYGGSLVRKHPKDEITKDEIKFWWTGREGKIDNNTTPGDPNSTEVATSTTTIGSINENLARMAKILYEVGAVDERLQILDVFSQTVKKLHKAMSKKENSDDSSEKESESGEMAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZH9</accession>
    <name>A0A9W8XZH9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZH9"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="142" mass="15907" checksum="EFEFC839D0314A49" modified="2023-11-08" version="1">MSPSKREKPIMIAKAAWKKVLETLLPQRKKVQKTQELGKSNTKYKQREAGHHSSTTLSTYTDTLRENENGVGQGGNTTPVWRHGEDVGHAKTVRFIEEEEIITALPPMPPRPVIEAPVVQAPGKLRKKRTMETAGNDVQRSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZN9</accession>
    <name>A0A9W8XZN9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_010318</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZN9"/>
    <dbReference type="OrthoDB" id="196103at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051617">
        <property type="entry name" value="UNC-93-like_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294:SF19">
        <property type="entry name" value="DUF895 DOMAIN MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294">
        <property type="entry name" value="ET TRANSLATION PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4363198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="132" mass="15195" checksum="0490057A513F8E14" modified="2023-11-08" version="1">MRQNRYGVEWFVYLGAVLCGISAGFFWSVEGAITTGYPENHKRGRYIATWFTFRNFGNIIGGAVSLSINHKTNHKGKVGYQTIGDLLRYSVWVSSLVSCFQIRRKWFEMMVPESKHQEESIGVWKAERCGVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y018</accession>
    <name>A0A9W8Y018_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RSE1/DDB1/CPSF1 first beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008978</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="20774at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="2">
        <location>
            <begin position="64"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1398" mass="156506" checksum="16741D4EC1BDA2C2" modified="2023-11-08" version="1">MEHQVQGLVLINNEWVSRPHDVYQIMARDRETDTVMVEPATKPAVQVPQLGILSRTIFASPMCKWILPANVRHRDLTDIALIGEDSVQLKEIRDYGHVRQVATKTDFRGRILAAGVFGDLREIPITKNVGSPLPKNQGLHRGRRSMTGDEEHHLPPEVIVLTLTSRTLLFLWARNTQTDTVSFSHKSIKLPAGSSRFDRFGTFLAIDPKRRAMAVAAHEGRFILYKTKTMETWRKEIREGKETIPIEDERILSVEGRIMHMEFLSSGSGQDNFHVVLLFMIADRGKTKITCFDWDSRQDLGTASVRTERVLIESVRLRAPINPSILLPLQPGHSTQKPRWVGWSRAPRNPEFAKEAFYIAREDGRIMYAERGPASTVDINEAGVWPYRIDTAFACLSIDSSEFAQLYPDVLIAGGAGNDGLLCKVGAWPAEYSYSLEYPGTNRFSFVESIPNWTPLTDFSVTKVTGVRTPYERERAAIFVANGTDPHGEISELRHGLHAFVDDSFSGMQGCTGLWVIDYGSQTVAGSGKRAKEHYATFAITMPLETLLVKIIRTPPENRGQLSGAWEDWHWSKIQIPNEGNNVMCDEETLSACPWSEHVSIQVQRDEARSLSRPGLNQIDSVSFNNSLLLAASRPTFPFIATAFREDGKVYLDIIHISQDGSLVQAENTRHQLTNDPTCIELLDIHGTPHVFLSTFDSTILLLRIDDRGAPSLVLEESLEHVAVDGFRMLCESAVILSSMQQQVLVCATRTGYLLSADLAIAKDGMSLLPKYNWLATNNTTDNQRLSWQCVKMGSTSAQIRRSQTDASVAFVSCGPEFCRVRCPRVHQSGLEIDSIWFSTRANPGYLQSPVTAMYQLPFFTDPDSPGRNLGGFLFAVAGDELLFSQLDSDIRWTGHDVSSPAQNDSRAVPRKIHTGSKPSNVMYMQSLRRMVVSTIEAKEERPPPNGYRILESKLQLLNTHDDKPLDEANLKPEEGEALPNRFLAAEYNLKDADFRVYTIVEWPFVDHQGKKYCLIIVGTGINTGLGKVMGRRLIFNAGRNGSKLQLQKESSYDNPVYCVAMWNNESTVSVIGKTLSLDFFDSQAGRWFKRGTKELPSPGIHVTVERPFVYVSTLQHSHICYKVVESDQEDRYDFEQIFTDSRERSCTHHLTLDLPHTAGEGKDTIVLLTDKNNASVSALYHPPEPTVRNASDTLFEAYLPRTIVRLQRGDIRPPWRRPCPAIPGILLDNILGACSDGTIYNLSLLNQPSLHLLRLIQNLIEAKSARDLSFQASMVNPRSGDIFDVLMNGAGGSQDDGKITARSVDPRIAERGDVGKRLGHVDGDMLVRWLDEGGDLAALVREGTERKDVQALFLELEGVDNEERGVLVDEDEDADARFERVAKEVRKWLGEVIMPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0A1</accession>
    <name>A0A9W8Y0A1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010915</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0A1"/>
    <dbReference type="OrthoDB" id="3689429at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="54"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4361974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="366" mass="41390" checksum="4D09EE27F649FBFA" modified="2023-11-08" version="1">MAARADGELTPARATPLGQDEGARSVSSSGTATDVGSADDSVDDKDDLKSKMMLLWAERKNTDLEQNLRESKNEKEALKAQVVELQAELHAVRAVSIEEHGQMVAAIEAKRLSQETAEEAKQHADRLRTKMRTMQGKNEGLVKEVKILQLKLEEAILRTEDTSGTAELIAQLEKTHAAYKDKIALLTEKNTQLINKNDELTAKHFAASETIKKNESDVNFYRAKCENKEHDIDRLKSNIANMRKEKQERESSIKITKANVAYYITCLDMADAGYRHFTCAHDNLGNVPDGRNTTQQAKAFTVATMLFKEWQHDKYFIEQYIQTRVYQVEHHRAHEKVNQGRGDFSENIYNTFLAVDKTRNATISGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0I2</accession>
    <name>A0A9W8Y0I2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Nitroreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">HBN1_2</name>
        <name evidence="8" type="ORF">N0V83_009393</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nitroreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0I2"/>
    <dbReference type="OrthoDB" id="2138173at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02140">
        <property type="entry name" value="Frm2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.109.10:FF:000001">
        <property type="entry name" value="Nitroreductase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="entry name" value="NADH Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033877">
        <property type="entry name" value="Frm2/Hbn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43035">
        <property type="entry name" value="FATTY ACID REPRESSION MUTANT PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43035:SF1">
        <property type="entry name" value="FATTY ACID REPRESSION MUTANT PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="FMN-dependent nitroreductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="7">
        <location>
            <begin position="11"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4363940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="204" mass="23052" checksum="0DC3A70C6EA7B0AB" modified="2023-11-08" version="1">MAPKTFLEAVQTRRTIYALNKDVPISDKQITEIAKQIVLHVPSSFNSQSTRIVVLLGREHDTFWDYVLEVLKPMLPAEQFPSTEQRVSGFKAGYGTALFLEDPEPVEALRKGFPIFAHHFGDWSEHTSAMHQFALWTALEAEGLGASLQHYNPIIDIKAQEHWNIPVDWKLRSQLVFGGKVADAGEKEFKPVEGTRLFVHGAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0L0</accession>
    <name>A0A9W8Y0L0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Calpain catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_009165</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0L0"/>
    <dbReference type="OrthoDB" id="424753at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF425">
        <property type="entry name" value="CALPAIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="5">
        <location>
            <begin position="175"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="615"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="663"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="620"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="663"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="714"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="753"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="active site" evidence="2 3">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="active site" evidence="2 3">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <feature type="active site" evidence="2 3">
        <location>
            <position position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4364569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="765" mass="86407" checksum="1EB377B15A5DA676" modified="2023-11-08" version="1">MSSVSSDITDIGKPQEAINKFWQNLITKRPTKVTKIFPSSLYAHLLPPPRKLGTITGKNAAESYQAAASECRARVKRIVRECHRTNEKFTDPDFDIQDLSDKNCLEGLKYWYNDQPAVVASSVSPSRLGNALSTLVQSDILSQNAAAIDFVRAAKLLNRNESDATIYDGPGSVHRVDWIFDEPRFEIDGFTSSDLVQGSSGDCWFIAGVAMIASNPDLVNRICVERDEECGVYGFVFYRDGEWIWTVVDDNLYLNYSDFDAYGDKYDPTGEKETKYKKNNQTGSGALYFASCADENETWLPLLEKAYAKVHGDYDAIAGGFSGEAVEDLTGGVTTKILTDRVLSKERLWKELLQVNKQFLFSASSPCAYGDDSDARRGLALSHAYSVIKAVDAEGEDGKEHKLVLIRNPWGKRANASMGEWTGPWADGSREWTPYWLEKLDHKFGDDGLFWMSYEDLLKRFDLLDRTRLFDEGWTVVQRWASVSVAWVTGYLNTKFRVEIKKAGPTVFVLCQLDDRYFRGLEGKYDYDLHFILQQENADTGDHIVRARGAWFGNRSISAEVDLEVGIYEVLPKIEASRDAYTTDVHEVVTKLAERNPQKLRQIGMNYDIANAKGVAEVSEEQKRKKEQKRKEAAEKIKKDNEEEENERVAFEAWKKEEKAEYEAWKKEKKGLEQSDKAKQETSKEKLNNDEAKKEESNVETGTVIADSVETEGSKANTPVATTEQARPEIIIDLAIRAKESESNTEPAIESITNEKDAQPKSRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0S9</accession>
    <name>A0A9W8Y0S9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008631</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0S9"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="3">
        <location>
            <begin position="43"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="286"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="286"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="354" mass="39379" checksum="CDDBE2DB367B69F0" modified="2023-11-08" version="1">MSKYDLVKQLDNGTGSRTITVVPRATYTAPSHHCSHGPRKSVILIMGATGVGKSSLVGAISGQPVAVGHTLSSCTSECQAWEYSAGPNHIVQVVDTPGFNDTKRSDIDILSSISSWLSLNDVSISGVVYMHRITDKRFTGASRMNLNILLGICGEHFLPHVVLCSTMWNSIPNEQVANEAAAREHELVSSAEFWGPVIGKGAKYMRYMGEEESGRAIIKSILEHSATHAMALQLELRNPSCTVEDTEAGRLITAEIRRKEEKLRQERLEQEEEERELREELALEQEAARREEQRQRVREAAAARRIEGRNTRDTRETRDNRSGGPDPMAVMGAEVSWRLGPLTGKMNFPSFRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0V4</accession>
    <name>A0A9W8Y0V4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Adenylyl cyclase-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SRV2</name>
        <name evidence="7" type="ORF">N0V83_009301</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">The N-terminal domain binds to adenylyl cyclase, thereby enabling adenylyl cyclase to be activated by upstream regulatory signals, such as Ras. The C-terminal domain is required for normal cellular morphology and growth control.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the CAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0V4"/>
    <dbReference type="OrthoDB" id="77251at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008179">
        <property type="term" value="F:adenylate cyclase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019933">
        <property type="term" value="P:cAMP-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.330:FF:000001">
        <property type="entry name" value="Adenylyl cyclase-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.70:FF:000008">
        <property type="entry name" value="Adenylyl cyclase-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.330">
        <property type="entry name" value="Adenylate cyclase-associated CAP, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001837">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013912">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013992">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017901">
        <property type="entry name" value="C-CAP_CF_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036223">
        <property type="entry name" value="CAP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018106">
        <property type="entry name" value="CAP_CS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053950">
        <property type="entry name" value="CAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036222">
        <property type="entry name" value="CAP_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006599">
        <property type="entry name" value="CARP_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10652">
        <property type="entry name" value="ADENYLYL CYCLASE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10652:SF0">
        <property type="entry name" value="ADENYLYL CYCLASE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08603">
        <property type="entry name" value="CAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21938">
        <property type="entry name" value="CAP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01213">
        <property type="entry name" value="CAP_N-CM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00673">
        <property type="entry name" value="CARP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69340">
        <property type="entry name" value="C-terminal domain of adenylylcyclase associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101278">
        <property type="entry name" value="N-terminal domain of adenylylcyclase associated protein, CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51329">
        <property type="entry name" value="C_CAP_COFACTOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01088">
        <property type="entry name" value="CAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-CAP/cofactor C-like" evidence="6">
        <location>
            <begin position="392"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="46"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="269"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="344"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="283"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="365"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="382"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00054756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00072052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4364704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="547" mass="58156" checksum="BDC15745B1F480B7" modified="2023-11-08" version="1">MAMASSDQELGARHGPVPAVAGDNNILTSMVRRLEAATSRLEDIASSSLPNAESSHGGASPSAPSALVAGDATPTPIAAPKSPAESVPPAIEAFDDLVDTELNKWLELSGMLGDVIGGQAKAVQQAFAAQRQFLFIANKAKKPDDRTIMELLKDLQANMEKTDEFRQSNREAALKDPLSMVADGVGSLGWVTIGVGGGPKPHEYINELFGGAQMYGNKVLKEYRDKPENKSFVEWVQAYYKLFKALSEYAKKYHASGVAWNSNGIDAKEAAKQVSSTSSSSNIPPPPPPPAGGIPPPPGPPPPPGPPPPPGAAPAAKKAAPDMNAVFGDLNKGADVTKGLKKVDASQMTHKNPSLRASAPVPTRSDSSGSLRSKSPAPPKKQKPESMRTKKPPKKELDGNKWIIDNFDSPTEMLEIDAEINHSILISRCKNTTIRINGKANALSLDNSSRTSLIIDSLVSSVDVIKCPNFALQVLGTLPTILLDQVDGATVYLSKDSLNTEVFTSKCSSVNINLPAEDDYVENPLPEQIRSFIRDGKVVSEIVEHAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y179</accession>
    <name>A0A9W8Y179_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Membrane insertase YidC/Oxa/ALB C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_008858</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y179"/>
    <dbReference type="OrthoDB" id="2148490at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="YidC/Oxa/ALB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF65">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX18, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="264"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane insertase YidC/Oxa/ALB C-terminal" evidence="8">
        <location>
            <begin position="130"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4365240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="330" mass="36887" checksum="2DC211722E02DAFE" modified="2023-11-08" version="1">MLSTRLLRQPACQLVARSAVTSQASICRPSIRSFHASAARQDPLLDALLFLPHEMMSFIHSTVPWYAAIPLTAFMTRAILVTTAGSWSRALTARYIGLHPLRQALAFQKRDEVMRRGDFSHPKEAVRAVSQEIKQMTNQLDKRWNVSLRGQIGWTFAQIPIFFAMAETIRQKCGARDGLLGLGFSAFRSEDAAAGAGEAVVTSSRWFEPSLANEGMLWFQDLLLPDPIGALPFLVSGLMFTNIYMTKNTVNSDGRWPTYVRRSLLLVSLLIGPMCQQLPAALLLYWAGSTSSVMLWNAWLDWKYPAPRGFMACKRPLQMPPVPATKGRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1F7</accession>
    <name>A0A9W8Y1F7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008148</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1F7"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4365529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="139" mass="15729" checksum="49B3009DA6BD5E8D" modified="2023-11-08" version="1">MELEDGPWVEESTEETALDDALEVALDDDDCDYETLLETKLVPEIVDVCIVEKVVRMVLEDREVELDEEVEDLIEDEETGLQLPKSETVKVEDAAADDVRVDDDDELLADDEELCTLDELRLVDDVELDFAVEEDAVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1G0</accession>
    <name>A0A9W8Y1G0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Metal-dependent protein hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MYG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1G0"/>
    <dbReference type="OrthoDB" id="10265310at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003226">
        <property type="entry name" value="MYG1_exonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11215">
        <property type="entry name" value="METAL DEPENDENT HYDROLASE - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11215:SF1">
        <property type="entry name" value="MYG1 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03690">
        <property type="entry name" value="MYG1_exonuc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="39595" checksum="07B4F3FD6A2005AA" modified="2023-11-08" version="1">MATENPAKKLKVEAPVIGTHNGHFHADESLAVAMLRLLPTYMDSQLVRTRDPAVLSTCHTVVDVGGEYDDNNKRYDHHQRGFETVFPGHNTKLSSAGLVYMHFGKDIISAVTGLQDQDRDLLFEKLYTDFIEAFDANDNGVNVVAPKDLEKAGLEKKFEDRGFSIASVVNRYNYGVRSANAEETKTPEAKQAEEDMRFSKASQFVGEQFLIELTDRANSWLPARHQVKQAYDARLQYDPQGRILVLPEGMPWADHLYNLEKEQPIPSGVAPQVLYVLFPEDKPEGKWRIRAVSKENGGFENRKDLPDAWKGVRDEQLDQVSGIQGCVFVHAAGFIGGNKSFEGALAMAQKALEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2B6</accession>
    <name>A0A9W8Y2B6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH:ubiquinone oxidoreductase-like 20kDa subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_008893</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2B6"/>
    <dbReference type="OrthoDB" id="268400at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12280:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit B 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01356">
        <property type="entry name" value="NDH1_NuoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006138">
        <property type="entry name" value="NADH_UQ_OxRdtase_20Kd_su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01957">
        <property type="entry name" value="nuoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005012">
        <property type="entry name" value="PRK06411.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995:SF14">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56770">
        <property type="entry name" value="HydA/Nqo6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01150">
        <property type="entry name" value="COMPLEX1_20K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase-like 20kDa subunit" evidence="5">
        <location>
            <begin position="64"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4365274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="189" mass="21262" checksum="596708F1C667BF61" modified="2023-11-08" version="1">MKSPAIIPGNPGTPPPMKQARREVPLPSQEGKKGAMQYALTTLDQVANWARQSSLWPMTFGLACCAVEMMHLSTPRYDQDRLGIIFRASPRQSDVMIVAGTLTNKMAPALRQVYDQMPDPRWVISMGSCANGGGYYHYSYSVTRGCDRIVPVDIYVPGCPPTAEALMYGIFQLQKKMRHTKITRMWYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2S2</accession>
    <name>A0A9W8Y2S2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009473</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2S2"/>
    <dbReference type="OrthoDB" id="5126878at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053178">
        <property type="entry name" value="Osmoadaptation_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38111:SF2">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G01560)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38111">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="6"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="622"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="703" mass="78900" checksum="49D0B857BDC041F3" modified="2023-11-08" version="1">MTQTGKCDTCRQRKVKCDEERPKCGACRKRDRPCIYSYGKASAFVVQDPNQLTKHGKSKITPVMYAIESSVDEEFSSSSTPSALRITSEREAGNGLGFFQTLAPISNPRVRPSKKQVAHQKRALEAYLQQLQEESALIPIRPSSPETTLIARYISMLGSESAEKQPLSILGTWISSIPSRIGSSRMLDLAVEFLVNSYAVYWDETHSKRKLASATKAKALKELQLVVLNAQGKPNYEVLLSTKMHYAAEALMGIDTMYHAIHAFGLAQLLTDGAVSNIDDEHFWNLIDNTYIDDVSLSYCHSDSIPDSFQVNEAMLAGRKSVYDNEFYLSSTYPPAFDSDVMLLSASQRASMAIMHVFIQCPRLVGLVRHAIENPENTSALAAAVSLAESLWLLDLSKQVSGLVQTSVTKFPVPISTDLADIMPESLEFDSVQSMILCTRYWMLQNVLCGLVDALYVHFPTETASSLLPRPKGLRNIDVEAALQLGKSLPWAQSVSQKLPLVPLRLHTPLQISIGPWHRTLRDLGVNRSVNPDLGIATTQEISYEISRAERMKAWLIDECNSIHKQWGVSVVDEKPLLEALESMAGEKIPDWLPVRVRFEAEDGEMVMKLDYENRTGSYQERYDLGEKPPKRSTHPALREGRWQRENLSDEICAQHRDGGPTHPPHLQFYSTARIKRRHFHNSKKTKLLDLDFHSKTRIATLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2Y9</accession>
    <name>A0A9W8Y2Y9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2Y9"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="110" mass="12476" checksum="370489ABF9A6D18A" modified="2023-11-08" version="1">MSTDVERETLLEADVERDALEEADDDRLAEEVRETLEELAVDDREAEEVRDTLEELTVDDPELDAEREALLETLLEEATEDEAAEDDAEDDATEDEETEEDAILALPHFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3B0</accession>
    <name>A0A9W8Y3B0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008527</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3B0"/>
    <dbReference type="OrthoDB" id="542013at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF152">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="35173" checksum="61374D0A41458410" modified="2023-11-08" version="1">MSAYKTSILVTGGTQGLGYQTALKLAKQQPSTLIVIASRTETDKAAERINKALKQSNVIYMALDLGSLAKVRDFAQRWDAAGHSPIQALVLNAGLQFPGGIEYTEDGIEKSFGINHVGHALLFHLLVPRLTNDARIVVVASSVHDPAKKWGLTPAYTTPEGVAHPSPEAIKASNGRDRYATSKVANVLWTIALGRHLASSPNHKEKTVVVLDPGLMFPTHLSRDAAWLVKFLSLHVAHHLVPVLKILVNDNINTATESGGNLAWLAVGDGVRGKKGVYFEKRKETEPSKQAVQEELQEDLWRWTIETVAQSPEEKERFTSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3D0</accession>
    <name>A0A9W8Y3D0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WLM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008557</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3D0"/>
    <dbReference type="OrthoDB" id="447842at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07344">
        <property type="entry name" value="M48_yhfN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053136">
        <property type="entry name" value="UTP_pyrophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013536">
        <property type="entry name" value="WLM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30399">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YGJP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30399:SF1">
        <property type="entry name" value="UTP PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08325">
        <property type="entry name" value="WLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51397">
        <property type="entry name" value="WLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WLM" evidence="2">
        <location>
            <begin position="10"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="459" mass="50675" checksum="960ACFCE74BA64E3" modified="2023-11-08" version="1">MPLGFERLNERTQRPNALINFIRPLPGSTSAVAEQILNRVAAICYPFMKANMILVQALEEFPYNTEFVGRNFNAGEVIQLVLRDKNGRWLPQRMIEMVMVHELAHCKQMNHSKAFWKVRDEYAVDLRALWAKGYTGEGLWGRGRDLLTGAVQMHEGDAVDVPEHLCGGTYGRRAKKRKRGAKDKPTLSYAERKQRRILRKFGAGGQALGADDDTKVKLEGGVPKKGKPRVAGSARGRDLRAAAALARFDTAKKEDVSIKKEETPSDSETEDEFVEGDQEGAAVDVDGKQMLDDKGRALVKICEDEDDKDGNAMRELEEIQGFSTQRQPSTKPPDRSSIPTISKPNAKHDSSNEKRIKGDRSNGTPSQVTYHPFNSTKSNSSAQPTRPRVSEPARPVSCPLCSFQNDHGSLTCGACSNVLNPVLVPDHWRCRSMVCKGSEYVNAGDVGICGVCGTSRSCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3D5</accession>
    <name>A0A9W8Y3D5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AA9 family lytic polysaccharide monooxygenase</fullName>
            <ecNumber evidence="6">1.14.99.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Endo-beta-1,4-glucanase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Glycosyl hydrolase 61 family protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_008352</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Lytic polysaccharide monooxygenase (LMPO) that depolymerizes crystalline and amorphous polysaccharides via the oxidation of scissile alpha- or beta-(1-4)-glycosidic bonds, yielding C1 and/or C4 oxidation products. Catalysis by LPMOs requires the reduction of the active-site copper from Cu(II) to Cu(I) by a reducing agent and H(2)O(2) or O(2) as a cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[(1-&gt;4)-beta-D-glucosyl]n+m + reduced acceptor + O2 = 4-dehydro-beta-D-glucosyl-[(1-&gt;4)-beta-D-glucosyl]n-1 + [(1-&gt;4)-beta-D-glucosyl]m + acceptor + H2O.</text>
            <dbReference type="EC" id="1.14.99.56"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">Has a modular structure: an endo-beta-1,4-glucanase catalytic module at the N-terminus, a linker rich in serines and threonines, and a C-terminal carbohydrate-binding module (CBM).</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.56" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3D5"/>
    <dbReference type="OrthoDB" id="9971669at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030248">
        <property type="term" value="F:cellulose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21175">
        <property type="entry name" value="LPMO_AA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17327">
        <property type="entry name" value="MFS_FEN2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000013">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000018">
        <property type="entry name" value="MFS transporter permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005103">
        <property type="entry name" value="AA9_LPMO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF53">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03443">
        <property type="entry name" value="AA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="327"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="378"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="412"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="444"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="54"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4365732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="687" mass="74715" checksum="884581571053C699" modified="2023-11-08" version="1">MSSTGIGQERNPATIEKGEQILYDEAIGSDRSTDDLPWTQEEETAVRRKLDKVIVPLTTFLYLLCFLDRANVGNARIQGMAKDLNLVGVRFNWVTSIFYIVYMFVEVPSNILLKKIGPKYYLPLLVVGFGFVSLCTAFVHSFEGLLAARAILGVFEGGVMPGLAFFITCFYKRNELLFRIGIYVSAASMAGAFGGLLATILARIPPWGISSMIIHSWRNIFFFEGLFTMLVGLGAPFLMPRSPEDCWFLNERERMIATQRLVLKGGGAENEKVEVHHIKRAILNITNYFCALGFFFINITVQGISLFMPTILNDLGWTATKAQLYSVPPYVCACLIAIAIAYVSDKTNRRGIYLAVFTLPAIAGFSIMRWASDPDTRYAGIFLITIGAFPGGPGFLAWAANNAAGPAVRSVSTAYVVTLGTAGGVLATWTYTSKDAPKYHTGHTINLCGQIAVLVLSCGGIAYCRWENRQRNLDTFPRLVVDGKAEEKDWSATRETKNANTKQGVENPTVADIRCYSSKNAANVVTVPAGATIHYISTQQVNHPGPTQYYLAKVPAGADVKSWDGAGAVWFKISTTKPKIDAAKQMTWPGQNEYKTPNTTIPNATPSGDYLLRVEQIALHLAMQANKAQFYLSCSQITITGGGSGTPAPLAALPGAYKSTDPGILVNLGSLAAGDDYQPPGPAVWSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3F4</accession>
    <name>A0A9W8Y3F4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lea domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3F4"/>
    <dbReference type="OrthoDB" id="4023585at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="11057" checksum="8FA2073A4328DC19" modified="2023-11-08" version="1">MFRQQIVRQVRLFSTTPIARKSPVDSVKDAAKAVDRTVSDIAVKGIEKGEQVAGAVKEAVPETKGQAKGQANELAGEAKGKAQELKGEAKGKANELKGEADRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3F6</accession>
    <name>A0A9W8Y3F6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Gluconate transport-inducing protein required for gluconate-H+ symport</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TOS9</name>
        <name evidence="3" type="ORF">N0V83_007544</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MIT1/WOR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3F6"/>
    <dbReference type="OrthoDB" id="5319641at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018608">
        <property type="entry name" value="Gti1/Pac2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28027">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28027:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09729">
        <property type="entry name" value="Gti1_Pac2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="191"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="382"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="522"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="220"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="389"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="438"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="526"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="593" mass="65786" checksum="D06634894D586B47" modified="2023-11-08" version="1">MPPFGHDMSMAGVPKVEGYSDAEAWNWPQHSSSQHPSYVNVFDPSAYANFAPQIPPSSRGPPRHHSLVTSHALTTGTETPPPARPQVAGSLVKAQPAFKPTWRGFLDTTKDAMTVVEASLQGRLNHISRRPHDKERAEMLTSGTVLVYEENASGIKRWTDAVHWSPSRVMNNCLIYRQLVRALKPEEKKTALNPSCGTKRKRKEGSGPAMTKTGETLTNSDDEYEDPAFDGTPDGDADSMNKVYANFAQSLTPDQQRRFCGSLIDSYEFKEGGLMKKTISVKYQGTHHHVISYYSLEDLVNGKLKRPFEDPRLADIQPRPELLRGWKVSLEDEESKDMPLVSGYPHQIQPSHSQHHVVAVVTQAVPGHAVVQPPAPLHLHVPDYWHGPQASQYTPNHHTQQHYASPQSAEPQYSNTRPGPQYHQQQPSPQDYAPQLPPTQQYAQSLSPQTSSPQFSAPTAPPQPYPTQAQAARHYSFDQAQTAPAPPYAQQVPAPQYTLSHRHQAPQSAQYNLPQAATFDVHAPVSHSSPSTQQPGQASASQQDPQRRSSAPLSLVTYNQPQFPAAPYPQSYYVTDAKYMPEQKHAPDSNNGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3I3</accession>
    <name>A0A9W8Y3I3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein mS33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_007516</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3I3"/>
    <dbReference type="OrthoDB" id="2257454at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013219">
        <property type="entry name" value="Ribosomal_mS33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13362">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13362:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08293">
        <property type="entry name" value="MRP-S33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4366986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="106" mass="12427" checksum="9787994592CF2332" modified="2023-11-08" version="1">MAVPRQRILDLMRVQCRVFNTTYNPERLRLGSRVIHQRLKGPSVASYYPPRIGTISQLRSLYPEHQLIDEDEEDWLEHLNVAKSRGKGAPKKKRTAAESKKFNKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3Q4</accession>
    <name>A0A9W8Y3Q4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sucrose transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_007354</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3Q4"/>
    <dbReference type="OrthoDB" id="28755at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008506">
        <property type="term" value="F:sucrose:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432:SF35">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 45 MEMBER 3 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432">
        <property type="entry name" value="SUGAR TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13347">
        <property type="entry name" value="MFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="361"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="412"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="498"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="522"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="591"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="623"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4366828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="663" mass="72698" checksum="03E1C329AF6CD557" modified="2023-11-08" version="1">MSSSRPDGAESPSHTPPLASPRDPPKLDTASRPRPRPKLVSFGRTSLSDGRPTADGRPHVEFIHEDSPQIIPTRMSSEQDPLLKEVLSPLSDDDWQGEHHVEETKSSWFLFLLTFGGLGLQIGWSVETSNGSPYLLSLGLSKSMLALVWIAGPLSGVLVQPYVGLKSDNCRLRWGKRRPFIIGGAAATILSLMVLAWAKEIMGGFLGIFGADPESNGVRTCIMLFAVLFVYVLDFAINVIQAGVRAYIVDVAPTHQQESANAWLMRSAGIGNILGYLAGYIKLPEYLPWLGGTQFKVLCAIASFIMALTVGISCATCAERDPQFDTAPAEQKEGVVAFFKGLARSVKKLPPQIKRVCEVQFFAWIGWFPFLFYITTYVGEIYADPYFEKNPHMTDEEIDQVWEDATRIGTRALLVFAITTFAASVILPFVIPPTFQAPEPDRPMTPATPMTPATPHSMGGSGYFALSHTPRGSPKTLSEKIAKSLDMLQIKTLTLRRSWFISHILFAVLMVLTFVVRSTWGATILVGAIGIPWCVTNWAPFAIISSEISKRDAIRRGIIRPRDRESQLIAAGEDDGSGADSAGVVLGIHNVAIAAPQVIATLVSAVMFKLLQKPRGTAGDDSVAWVLRFGGICALVAAWLTLRVNEEKEEPEEEEPFRRRRMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4C4</accession>
    <name>A0A9W8Y4C4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N(6)-L-threonylcarbamoyladenine synthase</fullName>
            <ecNumber evidence="1">2.3.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">N6-L-threonylcarbamoyladenine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">KAE1</name>
        <name evidence="12" type="ORF">N0V83_007703</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L-threonylcarbamoyladenosine(37) in tRNA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4C4"/>
    <dbReference type="OrthoDB" id="10254073at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:tRNA N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd24132">
        <property type="entry name" value="ASKHA_NBD_OSGEP_like_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000038">
        <property type="entry name" value="Probable tRNA N6-adenosine threonylcarbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000295">
        <property type="entry name" value="Probable tRNA N6-adenosine threonylcarbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01446">
        <property type="entry name" value="Kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034680">
        <property type="entry name" value="Kae1_archaea_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017860">
        <property type="entry name" value="Peptidase_M22_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03722">
        <property type="entry name" value="arch_KAE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF14">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01016">
        <property type="entry name" value="GLYCOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Gcp-like" evidence="11">
        <location>
            <begin position="35"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="135"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4367173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="352" mass="38202" checksum="A88B56641F01EEBF" modified="2023-11-08" version="1">MIAIGLEGSANKIGIGVISHPSPDKPPIILSNLRHTYVSPPGEGFLPKDTAIHHRAWVVRLIKQAVRQAGVKIEDIDCICYTKGPGMGAPLQSVALAARTISLLWGKPMVGVNHCVGHIEMGRAITHATNPVVLYVSGGNTQVIAYSAQRYRIFGETLDIAIGNCIDRFARTLMIPNDPFPGYNVEQLAKKGKNLVDLPYGVKGMDASFSGILAAADLLAKGLDESLPDEKRLKTEDGELVTREDMCFSLQETIYAMLVEITERAMAHVGSQQVLVVGGVGSNERLQQMMGMMARDRGGSVFATDERFCIDNGIMIAHAGLLEYGTGVVTGLEDTTCTQRFRTDEVYVGWRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4T0</accession>
    <name>A0A9W8Y4T0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4T0"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="143" mass="15545" checksum="EA451AD80E6A253A" modified="2023-11-08" version="1">MQGEGNQESLNIMGDVDLGLQWWPTDEEADPRHTSNDILQAEDQIDDPDQIAQPGLPTNLSIPESPKDHSTYSHHDSFSDTGLVDYNLEFGGQAASNLLGMTGGSETWTVPTPFMDSTSPQSHPFSSPSDVHQNYQADAESLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y505</accession>
    <name>A0A9W8Y505_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">14-3-3 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y505"/>
    <dbReference type="OrthoDB" id="5370350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="384"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="544" mass="59270" checksum="B736D59947892898" modified="2023-11-08" version="1">MASLDFDQKFLGRIAKQTADSNPFLSGALYQILGLSVLLARRLIRARKLRKLDTSRDTPSLASYQHILWISREGLSILELYVLPYAQDNQHGPECRVLSVKLRASFYHIFCLFHNHPPVTTTNMATADPRLPGAPHVPLSPRNGNGHGRKTDPSGLSPSSRRAGKQPTLREPIDSIVSETSFVTNPYATGGPVGTPSPGPPINAPPGLNPIPIPQPSSFILPPLNFIPLASGYFSTATSYATQFLPGSHPLRLSVALEHSAFLWDCVHDHEASRRVARRAIKDVYRAQEAMDDTEFEDAAELVGILGRMMKRKSWEGTPRVGGMRSPEPATRDAAQASGGGNEQSNIPTVAPQQGSPQGSRRAAERPPITDASGQSQSRDPVRSRSTSTSKDTTSRGWKSRDDTTHEAYQCGKRRQHNSEGASGGWSLARDALDSANHTPAFGQRRVTKRDSSHRKLYAHLTECRRHTRVRCSSQLADSSSLTTHAILAIPRKYIHKAAKVTSGDGTGKIKEDGSAVDRVRSRGRHRAASVDAAFVGVVHARGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y546</accession>
    <name>A0A9W8Y546_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Integral membrane protein DUF92-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_007231</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y546"/>
    <dbReference type="OrthoDB" id="15001at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002794">
        <property type="entry name" value="DUF92_TMEM19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01940">
        <property type="entry name" value="DUF92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4367646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="37872" checksum="B1943CCB69E365C8" modified="2023-11-08" version="1">MKPIIAIPAIAALVYRAYSRKSLTPVGIFTAFVTAIVHALHPWSVFFALLAVFFLAGTTVTKVKHDIKAKLTQSATGAAGGEGSRNHIQVIANSGIASVLILLHYLQLRKEGRYDKEDLCWSRGSDALVVGIVANYAAVAADTFSSELGILSKSKPRLITAPWRVVPPGTNGGVTTAGLGAGLLGAFILSATSTLLLPFCKDWDLAEKAQYTVALTTAGFSGTLLDSLLGALFQASVVDVHSGKVVEGAGGSKVLIHSSNPLHLSKTAQARSKVIKYEEGKDGIAKSSGADVATSLKAAETMQKANASGTAVSDGQHESRKIEVGSDILDNNAVNILMAALVSLGAMLAACAIWDLPYSSIVGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5B6</accession>
    <name>A0A9W8Y5B6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_007517</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5B6"/>
    <dbReference type="OrthoDB" id="2392202at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207:SF0">
        <property type="entry name" value="RIBONUCLEASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="RIBONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="3">
        <location>
            <begin position="114"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="380"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="436"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4366987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="466" mass="52871" checksum="E6D4E4077B91F0FE" modified="2023-11-08" version="1">MSAPKRGGSFNHYGDLSPQFNKRQRPNRPVYSSATYHNAPPYKPQLSSAEMKTGLVALLDRFVAEETTPAADKDILHHARELRRLLCARNDSNSSSKRELDEKRPDKGLKVFVPPYLERKLQAAKDLPPLPPIAEPHLHEAVFTHRSVHVSAGDPIQRHNIDLGLDYERLELLGDAYIELIASRALYNRFPHVDVPELCSWRERLVENSNLGRFSEAYGFPDRLNSRTNRDPSSKAWQKVVADMFEAYVAALVLSNPISGFQVAELWLTELWAPQLLGFREKVIENPQARDELNRLLIVKDVKLNYREEKPMTYDSGSIQRYYMGVYLTGWGYQDEWLGSGEGQNKPQACVAAASAAIKSSPVVKSVAQQKAELLEVKRKQREEDEARQQEKTVSEEVTRGNHIPSSNVQDDSGDDVASKKRKSNAEPSSPEKEKEKKKKKSKKSKKDKEGRKERKHKTSTDDDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y645</accession>
    <name>A0A9W8Y645_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Acyl-protein thioesterase 1</fullName>
            <ecNumber evidence="3">3.1.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Palmitoyl-protein hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_006130</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Hydrolyzes fatty acids from S-acylated cysteine residues in proteins with a strong preference for palmitoylated G-alpha proteins over other acyl substrates. Mediates the deacylation of G-alpha proteins such as GPA1 in vivo, but has weak or no activity toward palmitoylated Ras proteins. Has weak lysophospholipase activity in vitro; however such activity may not exist in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y645"/>
    <dbReference type="OrthoDB" id="2418081at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000010">
        <property type="entry name" value="Acyl-protein thioesterase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050565">
        <property type="entry name" value="LYPA1-2/EST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655:SF17">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="Phospholipase/carboxylesterase/thioesterase" evidence="13">
        <location>
            <begin position="6"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4369048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="238" mass="26430" checksum="8577A06AAB91E7A2" modified="2023-11-08" version="1">MAGKTPMVVPALKRHTATVIVAHGLGDSGAGWLFLAENWRRRSKFEEVAFIFPNAPNIPITLNMGMRMPGWYDIKSLSTLDDREEDEKGILASRDYFHSLIDQEVAKGIPANRVVIGGFSQGGAISLLSGVTYKNQLGGVFGLSCYLLLQKKIKDMIPTENPNQNTPIFMGHGDADQVVAHKWGKLSADELVKHGYKVDFRTYKNLQHSADPDEIDHLEAYLDQQIPPLGDIKQETNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y672</accession>
    <name>A0A9W8Y672_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y672"/>
    <dbReference type="OrthoDB" id="3790934at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="441" mass="48589" checksum="4348D2E8AE5F10D5" modified="2023-11-08" version="1">MSSLAAVRTALKSSDSEHATYLTSLIDSGKIAPSKTNPGSKTRQLFGPLDADFPRLGKRISLFDADRIPTKQELQELCLRDLKATREHFNDRLVNFAPSRAAEDATATDEGDPTLNLITNINKDRESSSQGISQAIAFQSESLQNICIEGAEKPDNVLHTSMTACIVLPHNNLLPLQHSNEGITTTTLLSGSIVWIIWPPTHHNLSILQTFYESLAEDTDDSVPDVSRDLEGGIAFLQNEGESLRFPPFCPMMNLAIKTSVLATSSTVTATAYIAILRRLPLLKSWFKTEVNGDQKQSEFNSAMLKTLDKMLNGEIDPATKELNVSHKLPLTPNDGPLHALLSSWDDAKGDVAAILSTADKKVVKDIWASFLIREKGRECKICGKVIRNKMKLMRQHFVDVHWPMEEKGKGKNKGEGRVDSMEVGGDELSGHGDEDAMEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y681</accession>
    <name>A0A9W8Y681_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Deoxyhypusine hydroxylase</fullName>
            <shortName evidence="9">DOHH</shortName>
            <ecNumber evidence="9">1.14.99.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Deoxyhypusine dioxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Deoxyhypusine monooxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">LIA1</name>
        <name evidence="11" type="ORF">N0V83_006569</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[eIF5A protein]-deoxyhypusine + AH2 + O2 = [eIF5A protein]-hypusine + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:14101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="Rhea" id="RHEA-COMP:12592"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="ChEBI" id="CHEBI:91175"/>
            <dbReference type="EC" id="1.14.99.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="9">Binds 2 Fe(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the deoxyhypusine hydroxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.29" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y681"/>
    <dbReference type="OrthoDB" id="421002at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019135">
        <property type="term" value="F:deoxyhypusine monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03101">
        <property type="entry name" value="Deoxyhypusine_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027517">
        <property type="entry name" value="Deoxyhypusine_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697:SF5">
        <property type="entry name" value="DEOXYHYPUSINE HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697">
        <property type="entry name" value="PBS LYASE HEAT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4368213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="329" mass="35765" checksum="2DA3752B09F4CD9D" modified="2023-11-08" version="1">MAVTEQDTQVPTLRQILTSESEPLARRFRALFSLKHLASLQPPNAQTVPAIEAIAAAFSSPSALLKHELAYCLGQSRHDAAIAPLRGVLEDKDEDSMCRHEAAEALGALGDTGSLSLLKELRDDVKEVDVVRETCDIAVDRIEWEHGLQKDQEKLKKSDFTSIDPAPPLPQNTESPSIADLEKTLLDTSLPLFHRYRAMFALRDLSSPPDLPTAVPAVHALARGFSDPSALFRHEIAFVFGQLSHPASIPSLTEALSNANEASMVRHEAAEALGSLGDEEGVEDTLRKFLHDPEQVVRDSVIVALDMAEFEKNGEVEYAIVPGAQAVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6F2</accession>
    <name>A0A9W8Y6F2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gamma interferon inducible lysosomal thiol reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_006689</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GILT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6F2"/>
    <dbReference type="OrthoDB" id="958254at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016671">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004911">
        <property type="entry name" value="Interferon-induced_GILT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234">
        <property type="entry name" value="GAMMA-INTERFERON INDUCIBLE LYSOSOMAL THIOL REDUCTASE GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234:SF8">
        <property type="entry name" value="GAMMA-INTERFERON-INDUCIBLE LYSOSOMAL THIOL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03227">
        <property type="entry name" value="GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4368333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="256" mass="29013" checksum="1EA7E0C1CD34414F" modified="2023-11-08" version="1">MDVKKPLLPHAQHALQDDVNAEQDVTTMARRRSNKTRFQLAAVAILLSLFWLARTWNCDHEHLELDTKVPLEVHIMSKCPDARECLKKLVLPTMSNASEKVDFRLSFIGRVTNEDDGVQCKHGQTECLGNILMLCAAAEYPDPKLYLGFSNCLINDYQEIPAKSLVQDCALEHGLDFDVLNDCMSRDSGGYGMGLLRDSVQHSKDVGVDTSCTIRLDNKVRCVYDGEWKDCDKGDKPEDLIRDIERLYDEAQGWTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6K4</accession>
    <name>A0A9W8Y6K4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6K4"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="176" mass="20366" checksum="C201A984E70D1685" modified="2023-11-08" version="1">MLSITCQIDDGDKASFTRIQIMITSTLVLSLIELLALTNTPIPYLKRIDRLRPYYMRKWYGTRRPYSRLDEESLPSHNEYQLRSVRFPQIPLGWLAFAELSAITLVVYSIVELLNSDTFDHPEQQDSSADVSRPVMMTTDAFVLQVLAFFCHVGVFFTALWHTLSRKVNNWSSLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6S5</accession>
    <name>A0A9W8Y6S5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_006402</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6S5"/>
    <dbReference type="OrthoDB" id="413079at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000286">
        <property type="entry name" value="MFS efflux transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000308">
        <property type="entry name" value="MFS efflux transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051788">
        <property type="entry name" value="MFS_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23514">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23514:SF6">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="328"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="395"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="113"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="13"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="56"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="513" mass="54854" checksum="F6A610655DAD1BD8" modified="2023-11-08" version="1">MSSSAFHSLFDIQPASNVASPLPTHQKNNGKAPQSMDDIGVLPDVELEDLASGKIYEDSKVSGTQTPGYHGPAGTQTPKTPNELEMSRPPTPKRDEAVGLIRTWNNPPMTKWRILCCCLAYFSNGINDSVVGALIPYIESHYHISYSIMSLVFVGNAVGFITAAFFTNIILGKLGRAKTLILADLIQLSANIILVCTPPYPLVVISFFLMGYGAATNLALNNVYCAHTHPSSVILGLAHGSYGVGGIVAPIIGTAIVSSGVLWSRFYFLSVGLRLCCIAFAAFAFWSYTEESEPNLITALEATASRQTAAEEVVSKLKDLKLALKNKVTIFGALFIFAYQGAEVSISGWVISYLIKYRDGDPAKVGYVTAGFWGGITLGRFVLTHAAPKIGEKNYVVILTLGTIVLQLLAWLTPNLIGNAVAVSLLGLLLGPVYPCAQTIFSRLMPRHVQTTAIGFIGGAGSSGGAVAPFTTGILAQAAESTRDQNNLSDMRHVGNFNAAREIKSCFDYCGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y741</accession>
    <name>A0A9W8Y741_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GmrSD restriction endonucleases N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006044</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y741"/>
    <dbReference type="OrthoDB" id="5419821at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004919">
        <property type="entry name" value="GmrSD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39639">
        <property type="entry name" value="CHROMOSOME 16, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39639:SF1">
        <property type="entry name" value="DUF262 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03235">
        <property type="entry name" value="GmrSD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GmrSD restriction endonucleases N-terminal" evidence="2">
        <location>
            <begin position="72"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="651"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="729"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="770"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="474"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="537"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="667"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="741"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="793" mass="87662" checksum="C97082C2C961EE34" modified="2023-11-08" version="1">MEVDPKPNAPKRLSNARPVGDSDALQRSLKNEMVDDDDLLESEDDEKSYKPRPQLPKPFVVMRTLAHLMRDLESGIIDVDPEYQREVVWTADRMTGLVNSLMENFYIPPIILNKKPAANGNGTQVTLVCVDGKQRLSSVRAFIKGMIPCHDHRGEKWWFCEMPGTRRKRVLSEEAQKLFLAKEFVSFEFTDLSPEQEEDLFARVQMGVQLSLAEKMRASTGPWQELARLFVVDFPTIYSLMKDRARAKDFQLTLSCFSQILEVMHPVSSSGIPLLKTNYTALPKFLSNKGAVDDGIKSHLASVWNTFEDLLEHDSDTFTNADKYLTGVQTFAPVEMVAVAVLISMYSSTRNNRLLLGDIKSLRIALREHFTDLRLHSQVWKFVWEYLADLEAIRGAVDGSTVDQRLLQEPSRHDPAIASTPPTRRDGPSTRTNPRSILPPANPVTVKREQGASTPPPEYRSPKRKRTNPGPDGTRSSVIDHSNGTVTIPDLPDGFFNADTASKGSRSGGSYLESRPPPSSALHDGPGSADHQRSRITSNVSKSTTKKSTPPTPQRTTTSNVQTSNTPEQIAPPTPGQIRQSRISELNDYRAPTTSMGAIDPIVPTSQHIPTARMYQTNTPRVSPPPVVRMGHMSSLTLRAGIGAFTPSHTEKQWEGELESVSPTMPQQVSSIATSQQASSATRPSQSRPAHRPRKSIPRPILAQYDGAIDLTSDSELEQERQNLLSSFKGMSNASKPTKDAAPASVPQPAVPSVSTQRVPVVVQKVEAEDIPPGANPYARFKKNQGTARFEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7I1</accession>
    <name>A0A9W8Y7I1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_005649</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7I1"/>
    <dbReference type="OrthoDB" id="5985073at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4369885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="128" mass="14948" checksum="53CD7FCB22283640" modified="2023-11-08" version="1">MDYIKYKYGLICLTEDGTSNYCSDVEENWNITTMVAQGRATWPQHTKKCYMDASNFNWEYTVDEEGNCTFPFENIWPNASDVAKPGEMAAMDYYMDVPDPIDDDNYGWPEPLEYDEYPLEMQCSSCFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7I6</accession>
    <name>A0A9W8Y7I6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ethanolamine utilization protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006223</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7I6"/>
    <dbReference type="OrthoDB" id="4985585at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02228">
        <property type="entry name" value="cupin_EutQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010424">
        <property type="entry name" value="EutQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36169:SF1">
        <property type="entry name" value="ACETATE KINASE EUTQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36169">
        <property type="entry name" value="ETHANOLAMINE UTILIZATION PROTEIN EUTQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06249">
        <property type="entry name" value="EutQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4369139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13311" checksum="89250A6867EC95A8" modified="2023-11-08" version="1">MPAMQYFEKAQSAFKPPLIANENAYLGDITTTESINPESPMSGGLYRLEKGTPLEYTYSYDELKIILEGHFYITDETGKEVKGEKGDVFMFPKGSKITFKTDDYGLAFFVGQRKKDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7M5</accession>
    <name>A0A9W8Y7M5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004929</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7M5"/>
    <dbReference type="OrthoDB" id="5398396at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="177" mass="18720" checksum="2B39A7A9E8553E6F" modified="2023-11-08" version="1">MGWNPFSTSPRRTSSSYRPGYASSSYSSSSRHHSSTSRYKRSPRDSYMQHLYKKLQHFLRELWRYAQRHPYKVFFMVIMPLVSGGVLAKLAKQFGITLPAALSGGAGGHHGARGSGAGGYAGSGHSSSYYGSEGYGMDSRGGGGGGMMSGLGNIDLQSVAGGIGGLASLASMASKFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7X1</accession>
    <name>A0A9W8Y7X1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_005716</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7X1"/>
    <dbReference type="OrthoDB" id="9936937at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058841">
        <property type="entry name" value="HTH_76"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040554">
        <property type="entry name" value="KPWE_PEX14_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36855">
        <property type="entry name" value="CHROMOSOME 10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36855:SF1">
        <property type="entry name" value="PEROXISOME MEMBRANE ANCHOR PROTEIN PEX14P N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25871">
        <property type="entry name" value="HTH_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17733">
        <property type="entry name" value="KPWE_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PEX14-like helix-turn-helix" evidence="3">
        <location>
            <begin position="9"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Peroxisomal membrane protein PEX14-like KPWE" evidence="2">
        <location>
            <begin position="120"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4369952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="182" mass="19552" checksum="F5481D5B03C5D7C6" modified="2023-11-08" version="1">MSVATDGLDDVYDALERYNWDDDVEFQSGLSAILGSNSSPDQAAELALRARCFYYARKYNKNIDFDAYKAYRNARNRPPPTPPTTNGVQAPLQDSLGPASTDTAGGIMPAPGNPSEPPAPYPTSFAQIVELITSGQPIPGIKEIPPTILTGQGTQPAQARRKKPWEKEEAATSGQQEGQAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8P6</accession>
    <name>A0A9W8Y8P6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Magnesium-dependent phosphatase-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_004515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8P6"/>
    <dbReference type="OrthoDB" id="2865258at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07501">
        <property type="entry name" value="HAD_MDP-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000155">
        <property type="entry name" value="Putative magnesium dependent phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010033">
        <property type="entry name" value="HAD_SF_ppase_IIIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035679">
        <property type="entry name" value="MDP-1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010036">
        <property type="entry name" value="MDP_1_eu_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01681">
        <property type="entry name" value="HAD-SF-IIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01685">
        <property type="entry name" value="MDP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17901:SF14">
        <property type="entry name" value="MAGNESIUM-DEPENDENT PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17901">
        <property type="entry name" value="MAGNESIUM-DEPENDENT PHOSPHATASE 1 MDP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12689">
        <property type="entry name" value="Acid_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01131">
        <property type="entry name" value="C1.5.2:_MDP_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4371298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="24036" checksum="6369ECD194AD2C6E" modified="2023-11-08" version="1">MPRRSAVTSTLSATDATTTTTAIPEKVEWPSTFSDGLPLPKVVVFDLDYTLWPFWVDTHVTGPIKAVEGGLKVKDRYGEGYGFYHDVGSVLDALKQKGILVGAASRTHAPDLGREMLKLLKVPSASSGSSSTRAIDYFDHLQIYPGTKTTHFQRIHRDSGVAYEEMLFFDDESRNKNVEVLGVTMRLVRDGVTRNEIDEGVRSWRARNGRTKAEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8V0</accession>
    <name>A0A9W8Y8V0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytokinesis protein sepA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_005423</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the formin homology family. BNI1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1104827at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.150:FF:000003">
        <property type="entry name" value="Cytokinesis protein SepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2220:FF:000006">
        <property type="entry name" value="Cytokinesis protein sepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000291">
        <property type="entry name" value="Cytokinesis protein sepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.30.50:FF:000001">
        <property type="entry name" value="Cytokinesis sepA protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.150">
        <property type="entry name" value="Formin, FH3 diaphanous domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051661">
        <property type="entry name" value="Actin_filament_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014767">
        <property type="entry name" value="DAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010472">
        <property type="entry name" value="FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010473">
        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47102">
        <property type="entry name" value="PROTEIN BNI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47102:SF2">
        <property type="entry name" value="PROTEIN BNI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06367">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06371">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01139">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01140">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51231">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="6">
        <location>
            <begin position="259"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="7">
        <location>
            <begin position="1083"/>
            <end position="1504"/>
        </location>
    </feature>
    <feature type="domain" description="DAD" evidence="5">
        <location>
            <begin position="1524"/>
            <end position="1556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="229"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="901"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="969"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1481"/>
            <end position="1734"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1373"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="170"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="901"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="981"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1005"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1022"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1481"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1516"/>
            <end position="1527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1541"/>
            <end position="1550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1551"/>
            <end position="1563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1623"/>
            <end position="1635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1718"/>
            <end position="1734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00037935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4369661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1734" mass="192275" checksum="DCBD852230AEEB31" modified="2023-11-08" version="1">MDGKGRQSSAGKFFGRRLHKDKEREKDKAGSDQRPSTSTNNITTDSPPGSSYGSQSSRHSHRHSTSAASIDRPVSMTAEGIFARAGPVSSIDPPRDDAARSNDPLPHHLNKGGGDFHQYPVFAATPMPQNGYSTPGPPRPPPHSSQTIASSQPGDRGVSTQQWGARESSRNGNYSYYAGTDSSNNTRASSDQASIYSTDSKTRGSNIFYPQNNSQTTFSSIGLDAGSLLPTATSTPRDSHRHLLHYNSPSSPSSSTASIQRPPDHVVEKEFMNLMVKRGWKSLPEQARRQMEAYKIDKKWTLVYQDKLAELKHEEKKRQTQRQTYGGGANPDILMRAEEEGSPEWYVKKVMDNSISIKQMSSLEISLRTQPIAWVRGFIEAQGQIALTNVLTKINRRKGQGPAPPPNSMAQKAENDVDREYEIIKCLKALMNNKYGADNALYHPSIIQALCSSLISTRLNTRKLVSDVLTFLCHWGEGSGHEKVLQALDNLKAQYGENSRFDAWMRMVEITVDGRGKMGSMVGASDEVRSGGIGVENLLMEYAIATLFLINMVVDAPERDLQLRMHIRAQFTACGIKRIFKKMEGFQYEVIDKQIERYLANEAIDYEDFLEKENSSMVDSVEGETKDLNDPSQIADAIMNKISGSRTQDYFVSTMQHLLLMRDTEGEDRLRMFQLVDQMLSYVAMDRRLPDMDLKQSLNFTVQSLLDKLYTDSEARQVRDEAIEARQIADSAIAERDEMKAQLELGADGLVQKLQKQLAEQERIIELRGRQVEQMKAELAEMQRIRAQELQRNELETRELYLMLRDAQDVAASAAKKSGKDGLGPSDPAQMQGIMDRERLMNRLEIQLQRARTQATLEGKTLQMQPSEKLRELREKMEGDMGAVPDQSFGGVDANNFGSIRRAKSGVPRRKPLPGLPGQLGEEDVMSEDDEDMIIEKPRLIQMHKPKLSASTANALMGEIASNVKRYDDSDAEDAGDGVTTGPSHPSLESDSPKTPADTESAKPNIPPPPPPPPGALGFPANIPPPPPMPGTNANIPPPPLPSARQAWICMPPPPPPPPPPMMPGAKRPGFLPKAPGFASTAALSGPRPKKKLKALHWDKVDQPSTTVWATRGLTSEEKEEKYQELSKKGVLEEVEKLFMAKEIKAIGKKSGKKDDKKQIISRDLMHNFQISMAKFSSYSVEDIVQMIIHCDKRILDDVVVMDFLQKNDFCDIPDNTAKLMAPYSKDWTGPNAGESKREQDPNELTREDQIYLYTAYELHQYWKSRMRALALTRTYEVEYDEISNKLLEVSRVAESLRDSSSLISVLGLILDIGNFMNDANKQASGFKLSTLSRLGMLKDDKNESTFADLVERIVRNQYSGWEGFTDEISGVITAQKINVEQLQADAKKYIDNIKNIQMSLDAGNLSDPSKFHPEDKVSIVVQRSMKEARRKAEQLQVFLEDMQKSYDDIMAFYGEDPTDDSARRDFFAKLANFVQEWKRSKEKNTSLEDQHRRNEISMRRKAAQAPNPLSPNALSSDSDAPKSPASTGAMDDLLQKLRAAKPEARDQRDRRRRARLKDKHQVRVASGQKIPELGVNMEEEGGGEAGAAAADDESNTDAKGKAMLSPTSDMADGEASGGGAAGTVTSPTGTSTDADVADRAAAMLEGLQGGGSLASLNKDGSLSVRRRRESADTERQRRRNRRQQAGTSGSSVGKSEDGGGLMSPSTTTIPEEYGDGGEGRRGRELPTPPPEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8W4</accession>
    <name>A0A9W8Y8W4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005314</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8W4"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="222" mass="23878" checksum="167D4FBCE4A7AEE0" modified="2023-11-08" version="1">MDARVLDATGHHDVYELSVPVSKSPLVRTWLMRAAEGDGEAETVEDADVNEPNDMTSDDSALGDREVLLSVGGTTDADDGVLDCCVNEELAAVEADEDGIGLEDDNPMVDKSLEEPTVEAPLAADIVLEDADTLNVEEGLGLDDAEPLDERSYDEGELSPSMEDDVVAEEELGMAADGELNELATEEDMRRAVENLADVDCEELDDDRIEVNWLALQSPNPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9E7</accession>
    <name>A0A9W8Y9E7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arginyltransferase</fullName>
            <ecNumber evidence="2">2.3.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ATE1</name>
        <name evidence="8" type="ORF">N0V83_006318</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the R-transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.8" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9E7"/>
    <dbReference type="OrthoDB" id="74183at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004057">
        <property type="term" value="F:arginyl-tRNA--protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030700">
        <property type="entry name" value="N-end_Aminoacyl_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007472">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007471">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367">
        <property type="entry name" value="ARGININE-TRNA-PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367:SF1">
        <property type="entry name" value="ARGINYL-TRNA--PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04377">
        <property type="entry name" value="ATE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04376">
        <property type="entry name" value="ATE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-end aminoacyl transferase N-terminal" evidence="6">
        <location>
            <begin position="11"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="N-end rule aminoacyl transferase C-terminal" evidence="7">
        <location>
            <begin position="140"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="308"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="308"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4369233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="428" mass="48925" checksum="9A3DA87CA4EA5FC7" modified="2023-11-08" version="1">MAVPNPGRASYYFSSKSLTVEVYQILVDRGWRRSGTIFYKPDVLRHCCPHYTIRLPVESFRPSKDQRRAINKWNDHVLGEAYMKEASKLYPLSKEEKARLKNTFDLNREVHKAELENVKQPPEPAHRFEVTLEPADFTLEKFELFKNYQQNVHKEKSHQISMSGFKRFLCESPLQQTSGQVDGKTQLLGSYHQCYRLDGRLVAMGVLDLLPHCVSGVYMLYHSDFEQWQFGKLSALREVTLALEGGYKYYYMGYYIHSCTKMKYKGDYKTQHVLDPETYEWNPLDDELRGLLDRMSYVSLSREHRRKDMGEVSARTKDGDADKGAEVEPKDLSDYPLPTASEAGAAVTKGMSLFDLKVPGLMTVEEIEEHLDLGTMPIRVGGRMAEAQDLVSWDSGDLRKPHSVKGIVGELVACLGPEAAWQVVVEFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9H0</accession>
    <name>A0A9W8Y9H0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004599</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9H0"/>
    <dbReference type="OrthoDB" id="5420247at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42029">
        <property type="entry name" value="AN04G07800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42029:SF3">
        <property type="entry name" value="AN04G07800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="296" mass="34161" checksum="B5BE82B3330DCD19" modified="2023-11-08" version="1">MSYFLHNVVAWLKIRPFLPQWGSRVFIISLLCVQPFWVVEAWSNFYYFNSLGSNVNEKTRPWEALFRDPWWIFTTWRLFYAIKKTYGFKLWGLLKINARFGVMLICMVLSIAFLLTDVAVTAAHLSANSGINPYWRFALVFKCASDTIFLDDFKSVLDDIVTRKSSSTKSSSNRRSIARSRSLSRTFSRTASRIDEIIECRALDNAPDTRVHTMTTFHSAAQKSKFSTLFSKKRRTEVPKIVIEQETTIGSQATQSNHDDFAAETPRPPRPAHVIYDNINLRASESERDLIFGRMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9N5</accession>
    <name>A0A9W8Y9N5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9N5"/>
    <dbReference type="OrthoDB" id="3801417at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="231" mass="26043" checksum="6E7EECB037D55DCF" modified="2023-11-08" version="1">MRSRIRRKQRHENAKCLKEAEKEVAVLSEKKNQDEFVPRSHKLYDLTKSVDIYVEEVLPMQESGKRARVSRQPQRIRLEPQMTSSEPRSSEPITQSTIVSSRESGPSMNESTASPKAPSTATEPPSDYLNYCSRCKGTCIRTEEHGTIVPTTLESDTGAVLANEGDDDDDDDGAQEPSDIEEASENDGDTNIKSNSVFQPVAGVKGRHPRGWHQRHERSESPQEFWHRKIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9W4</accession>
    <name>A0A9W8Y9W4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PKC1_1</name>
        <name evidence="12" type="ORF">N0V83_004684</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9W4"/>
    <dbReference type="OrthoDB" id="354826at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05578">
        <property type="entry name" value="STKc_Yank1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000354">
        <property type="entry name" value="AGC/YANK protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000469">
        <property type="entry name" value="Serine/threonine-protein kinase 32B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF422">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="20"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="376"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="527"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="382"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="419"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="442"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="468"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="540"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4371467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="563" mass="62951" checksum="1C821D62F8054CC9" modified="2023-11-08" version="1">MGQSNGKPVVFTDQVNLNHFRLLRVVGKGAFGKVRIVERKDTGLTFALKYIRKDEVVRSESVRNIIRERRMLEHLCHPFLCNLRYSFQDIEYLYIVVDLMNGGDLRFHISRKTFTEEAVRFWIAQLGCAVRYIHQQGIVHRDIKPDNVLLDSEGHVHLADFNVASDFTPHKPLTSKSGTLAYLAPEVYEGKGYSCEVDWWSLGVLFYECIYNKRPFEASSHDSLAQKILKGEPTYPVTSPPVSMPCLHAISSLLEKNRKQRIGAIGFETFVDNPFFRPLDFEALERKEIPPVFIPSSDKTNFDATYDLEELLLEEAPLEARARRQKPREALKDDATEQEIRAEELHKMIEQLFEPFNYTTVADQRPIAVAVVDPADSVGGSRASNQSASENTPASVTSQPDPWGRPLATTKSQDCDLQATRSTTNPRSTTQSPNGSPPLPTTILNQTGNTGSPTSQRGERSDYFPTDGSETTTPSFSRPMNNRNRGSQRSTSMGGGVQVVLNEHGSWTEMANQSSALVQNAQAEKPSGMLGFLSRKKGRDRSPKAKERGVLGKEGARVIISNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB50</accession>
    <name>A0A9W8YB50_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ABC multidrug transporter atrF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SNQ2_3</name>
        <name evidence="17" type="ORF">N0V83_005237</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>voriconazole(in) + ATP + H2O = voriconazole(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61912"/>
            <dbReference type="ChEBI" id="CHEBI:10023"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:61913"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001650">
        <property type="entry name" value="ABC drug exporter AtrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="574"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="598"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="649"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="681"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="711"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="822"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1251"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1280"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1322"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="16">
        <location>
            <begin position="215"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="16">
        <location>
            <begin position="906"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="82"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="868"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1196"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="10"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1196"/>
            <end position="1211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4370716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1366" mass="152629" checksum="1CA769E044764EDB" modified="2023-11-08" version="1">MWGTTIQTPQERDNRRNLWHEDEYLRNGREQTEELPDSGRSSEDSPNGLRRGGTWGERDAGGIDTRLAAEDLAVLREELASLSKTRSHGAQSERSHGLTRTLSRISTRQSHRAQRRASIATHRTTEGTGDDPDDEPGDIGAAAAEKEDEFELDQFMRDGHFEKRKDGKSAKKVGVVFKNLTVKGTGSTATFVKTVPQAIIGTFGPDLYRIVSRFVPALKLGRHKQTRVLLNDFSGVVKDGEMMLVLGRPGSGCSTFLKAISNNREGYADVEGDVSYGGIPAGKQKKRFRGEVNYNPEDDSHMAELTVWQTLKFALTNKTKKHDKDEIPVILEALLKMFGIQHTKHTPVGNEYLRGVSGGERKRVSIAETLATKSTVVCWDNSTRGLDASTALDYAKSLRIMTDMSNRTTLVTLYQAGEGIYELMDKVIVIDQGRCIYQGPASEAKQYFIDLGFQCPERQTTADFLTAVTDPSERQFREGYEDRAPMTPAELETAFRRSEAYQRVLKEIEQYEGDLKRSDYTDAKDFETAVRESKSKTVRKKSPFTVSFFRQVLACTQREFWLTWGDKITLYTKFFIILANGFIVGSLFYGQALDTSGAFTRGGSLFFSILFLGWLQLSELMKAVSGRDVVKRHEDYAFYRPSAVSLARVLQDFPLLLAQVIPFAIIMYFMTELDVNVSKFFIYFLFIFTTTFCVTSLYRMFAALSPTIDDAVRFAGIALNLLIIYTGYVIPKTLLLKKYIWFGWLYWVNPVGYSFEAVLTNEFSDRTMQCAPEQLVPQGSGVDPAFQGCSLTGAPHNSNSVPGADYLQTSFNYSRSNLWRNFGVVIAFTVLYLLVTVFATEVFSFAKSGGGALVFKKSKRAKKAVREEMTDEEKVVAGASSGDSSETAAHGEDEVLESISSSESVFTWKDVEYTVPYHGGERKLLNKVNGYAKPGVMVALVGASGAGKTTLLNTLSQRQNTGVVSGEMLVDGKDLGKSFQRGTGFCEQMDLHDGTATIREALEFSAILRQDKSIPKQEKLDYVDKIIDLLELQDIQDALVSSLGVEQKKRLTIGVELAAKPSLLLFLDEPTSGLDSNSAYSIVRFLKKLASAGQAIVCTIHQPSSVLIQQFDMILALNPGVYFGPVGENGNDVITYFGDRGVHCPPSKNVAEFILETAAKPIRRKDGTKINWNDEWQKSSNNAALLEEIDRIKSTRSKTVSDNRNHKKQDGGDEEEEEAEFAAPIWLQTTMLTQRTFTQYWRSPSYMYGKLFVAFIIAIFNGFTFYQLGNTIADLQNRMFTSFLIVLIPPTIVNAVVPKFYENMALWRARELPSRIYGWQAFTTAQVVAEIPAAVVSSVVYFVLWYFPTGLPMDSGTAGSRIYIYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBA0</accession>
    <name>A0A9W8YBA0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Multidrug resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">CDR1_3</name>
        <name evidence="1" type="ORF">N0V83_005313</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBA0"/>
    <dbReference type="OrthoDB" id="4093211at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4370791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="75" mass="7935" checksum="9BA8E4B1113566DF" modified="2023-11-08" version="1">MATIETASRSRNPIDLEIRSAENAPEEEVAASLGLTTALDTKVGNDFVQGLSGGERKRTTIAVKAPLILSSYTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBZ1</accession>
    <name>A0A9W8YBZ1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">18S rRNA biogenesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_003983</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNA 3'-terminal cyclase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBZ1"/>
    <dbReference type="OrthoDB" id="1911237at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.20:FF:000004">
        <property type="entry name" value="18S rRNA biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016443">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03400">
        <property type="entry name" value="18S_RNA_Rcl1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF1">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase" evidence="5">
        <location>
            <begin position="13"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase insert" evidence="6">
        <location>
            <begin position="209"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4372209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="407" mass="42968" checksum="CDCB821FCA2D72A4" modified="2023-11-08" version="1">MAADESLQVPLNFTGHRHLISRLLLATLTGRPVRISQIRSASTSPGLTRHETNFIRLLDAITNGSQIQFSMTGTTLVYRPGLITGSAEGLGAAGGVIRHEIPAECRRRGVSWWLVPLCVLAPFSKGGMNVIFHGEGCVTSATVASGDISADTVRTAILPLYKNFGIERNIELRILKRSCAPAGGKGAGGEVQLVFNHQVRLPKTLHLLNPGRVKKIRGVAYCVEVPKSNNERMIFEARGILNKFVPDTYIFSDASPAPLIPTSVKGHVSGKVKGAVGFGLSLVAESSTGCIYSADVCSPPDGGVPADEIGKQCAYQLLEKISQGGCVESVAAPTVLMLMAMGSEDVGRLALGKDILASEDIIQLARDSKVFGGSGWGFRENGGGNERGEIVVSVVGRGVGNVGRKVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCA7</accession>
    <name>A0A9W8YCA7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cation-transporting P-type ATPase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_002696</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCA7"/>
    <dbReference type="OrthoDB" id="158672at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005391">
        <property type="term" value="F:P-type sodium:potassium-exchanging transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050510">
        <property type="entry name" value="Cation_transp_ATPase_P-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294:SF21">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="159"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="553"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="582"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="627"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="678"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="716"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="749"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase C-terminal" evidence="11">
        <location>
            <begin position="577"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="34"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4373957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="786" mass="87497" checksum="8F02A9699F002D2A" modified="2023-11-08" version="1">MENEDNTVPRVRFGDQRLPDEEDGYVTHERRHQQGLSRQPSVGSLSIHSTGGFRTIKPETALPITYRTLSIEIDEGQQKKQDEARKVKEKAAVGSSLDHSSCITKLTSDPKTGITPLQKEMLRFVLIISLFITCVVILIVILWAAWLRKDHPTWIDVPLLIVNCVSAAVASIPEGLPIALTMSLTIVANIMRKNKILCKSLKTVETLGSVSVICSDKTGTLTKNKMTVTDIYAGGEEYTPEAARDLMAIFRSEKNIATMSKKREDIIDQVRILGGLCNSREFDAATMHLPISDRKINGDATDQAVMRLSESLGSVAELRRDWKKIFEIAFNSKNKFMIRLMTPANECATSNNTTLMIEGAPDILLPRWQSVILLARKPTVVPFSSNQEKEVLVAARYGLTFVGIVGIVDPPRDEIPDVVRILRGASIRIFMVTGDFKLTAQAFAEDCGIISNSALVDDISALGRDEIVFARTTPEQKLRIVKEFQARENIVGMTGDGVNDAPSLKAADIGIAMGSGSDIAIEAADMVLLDSFAAIVEAVQYGRLVYDNLKKTIVYLLPAGSFSELWPVVTNVAFGIPQILSSFLMIIICCLTDCAAAITLAYEKPEADVLLRPPRNPRKDRLVNTRLIFHAYFVVGLLQCLCSFTMAYWYLERQLKFGDYDANYDPEYIAEKVKTASSIYFVTLVVMQLFNLLATRIRRLGIFQQPPVFNKDTQNYLLFPSVIFAVVVVFIFCYIPSLQNTIGTRGIPVEYWFLPAAFGLGLLLLDEARKYCVRTWPNGFLAKIAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCB4</accession>
    <name>A0A9W8YCB4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Exosome complex protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_002565</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for exosome-dependent processing of pre-rRNA and small nucleolar RNA (snRNA) precursors. Involved in processing of 35S pre-rRNA at the A0, A1 and A2 sites.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the C1D family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCB4"/>
    <dbReference type="OrthoDB" id="1421013at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011082">
        <property type="entry name" value="Exosome-assoc_fac/DNA_repair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007146">
        <property type="entry name" value="Sas10/Utp3/C1D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15341:SF3">
        <property type="entry name" value="NUCLEAR NUCLEIC ACID-BINDING PROTEIN C1D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15341">
        <property type="entry name" value="SUN-COR STEROID HORMONE RECEPTOR CO-REPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04000">
        <property type="entry name" value="Sas10_Utp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="151"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="208"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="260"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="317"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4373826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="328" mass="36215" checksum="1F94F2AE7043349F" modified="2023-11-08" version="1">MDPQTDLPDLVEDLEVNIDELSTALQPLLSSPLHTTASSLPLLDKAKLYVLAAYSIESLLFSTLQASDVNAKEHAIFNELARLKGYFGKIKEVEERGSQPKQKLDVGAAQRFIKHGLAGNAKYDLERAERMAKERARAALKAKQINKKFDDEGKDKVEEVTPKKRAIEEVAAEEDDDDDDDFLEGFEEPEPATKKARIPAAEPMDIDSAPSPTSQPPSSSMEDRKQRSKKKAKQSADNTDAEELDTSANQDSDIPTVARQPRKKEKKTKGSKSKITRREPAEDVQEESILPDKSAPPMTRSEAFNSLLDGSRAEKAKKAKGKSKGKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCM9</accession>
    <name>A0A9W8YCM9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003656</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCM9"/>
    <dbReference type="OrthoDB" id="5419315at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43094">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43094:SF1">
        <property type="entry name" value="AMINOTRANSFERASE CLASS-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="60" mass="6624" checksum="2595D885554C2A65" modified="2023-11-08" version="1">MGLNSGIHVYPGGGTADGKNGDHIILAPGFDITFNELDFIVDRVSKLIEDYFNDFDIKNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD15</accession>
    <name>A0A9W8YD15_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD15"/>
    <dbReference type="OrthoDB" id="3793888at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4374347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="182" mass="20889" checksum="8A95CEF842C999A2" modified="2023-11-08" version="1">MHYHCLIQRATQPSNAFKDKCPVCHIQLFEWDGINALTLATRTGLELQDEKPGGYLDPNTGNWVPSDKTEYEADCAFLVTLVSRHLEAHSNQPSGFEDGSVDLARVYYDVCRDLDRMKKPQARWLQYRTRTGVFLLGMLVAIKMRRFLMEERPGVVGTEGWMEFEEEGRRAQERVLGEVHGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD53</accession>
    <name>A0A9W8YD53_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">3-alpha--hydroxysteroid dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001802</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD53"/>
    <dbReference type="OrthoDB" id="1669814at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24321">
        <property type="entry name" value="DEHYDROGENASES, SHORT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24321:SF8">
        <property type="entry name" value="ESTRADIOL 17-BETA-DEHYDROGENASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4374727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="26505" checksum="85CEB9F52BA8C31B" modified="2023-11-08" version="1">MSIKDKVIVITGAASGMGLETARLFASKGAKLSLADVQEKPLKDLEAELQQSGTQVYVQVVDVSNRKNVDDWIAATVEKFGKLEGACNLAGVIAKQSYVAGIEDLDDADWDFVFGVNVFGMRNCLRAQVPHMNEGSSIVNAASILGLIGAANNMAYCASKHAVVGMTRSAAKELGPKKIRVNCFCPGPIDTPMLQQSYKIKGSATDFGFLPLGRAADQKEVPPLIEFLISDAASFITGNAVPIDGGWYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDA1</accession>
    <name>A0A9W8YDA1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Palmitoyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.225</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PFA4</name>
        <name evidence="3" type="ORF">N0V83_003972</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDA1"/>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="278" mass="31392" checksum="87D09EA8EC6BB4CB" modified="2023-11-08" version="1">MPSYLGPSPFQLGHLFTTLIVNSITLFALGILLIRNVWCLAVNTTTIEGWEIERHRTLLRRARHFGGYLEGADGSQVRIKKQEFPYDIGIWANIVQGMAPTPSLQTGLSFPTNGFEDAGTTWPPPDPDRSYRRHAKAFNTDAFTYQDSNLGPQQTLNAFKARQAEDEVRRRKPFLERAEASLAKQRVEDWNGVGGEEDGYEYGDEASDVDDETREKYEDGEGQGGEEGWRNSEGERLQDFGVDEDVEFYDEHDDDDEVPLSELLARKQAASASVYMTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDG1</accession>
    <name>A0A9W8YDG1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Histone H4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HHF1_1</name>
        <name evidence="14" type="ORF">N0V83_001850</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the histone H4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDG1"/>
    <dbReference type="OrthoDB" id="3919494at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22912">
        <property type="entry name" value="HFD_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000007">
        <property type="entry name" value="Histone H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035425">
        <property type="entry name" value="CENP-T/H4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001951">
        <property type="entry name" value="Histone_H4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019809">
        <property type="entry name" value="Histone_H4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004823">
        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10484">
        <property type="entry name" value="HISTONE H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15511">
        <property type="entry name" value="CENP-T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00623">
        <property type="entry name" value="HISTONEH4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00417">
        <property type="entry name" value="H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00803">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00047">
        <property type="entry name" value="HISTONE_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="10 11" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA box binding protein associated factor (TAF) histone-like fold" evidence="13">
        <location>
            <begin position="28"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4374774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="103" mass="11400" checksum="2696CDB8AE0C4EFF" modified="2023-11-08" version="1">MTGRGKGGKGLGKGGAKRHRKILRDNIQGITKPAIRRLARRGGVKRISAMIYEETRGVLKTFLESVIRDAVTYTEHAKRKTVTSLDVVYALKRQGRTLYGFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDI3</accession>
    <name>A0A9W8YDI3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDI3"/>
    <dbReference type="OrthoDB" id="303614at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="401"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="477"/>
        </location>
    </feature>
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        <location>
            <begin position="524"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="251"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="529"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="558"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="602" mass="65563" checksum="AB81C24FD9B076F3" modified="2023-11-08" version="1">MSKPPLPSPNSRRDATTANTSLSPTSPTASRPPLQRLAASDASYQAQTSLLGSRAPAGDRDDETTDTDMSEDEETPGQGGAAGMGREASKVAPPHGKASPDETEIERLRSLQLKLLQQQQQQQQRAHLRGTQSPGPPFTASSRRISPIKEESITLSTMSPTLEGAFDSPSPLPTGHTTSTESTESARTIRGSMPPTPAGHQALRTPSYPFPTVPNTPRWASAFHMPFTNLSPTVSAINAREYTVPKERVASETSTPAASTTPFAPGERIQHSTEAEDPRFPSPNLYDLVLRLGSDPGLAAWWTAVTSIMCEHYGVERATLAVPADASDIENVPWGQKTSFTATTDDGSPRMATYQDVKVPQSRKESTQQQQGLQHFEQEYTPHTALIPERRPKLFSHHSFAGHERQRQHQATDSPTPTMPLRPRGPLRAASHAPQVAMREHPLRQVSRASFDGTSNPFPSATPSAGPTFSDPEFSSVGEPSSPCAVFNVLRALDHEPDALIDNTGINRVLERGRLVTLTRDYSTSFSTSREDLADKDNAEQHKSSTTQATFEGHDTLKTTATRGRTALTTSDPRLQPRYEEYEQYPSSPWAQSPAPLARDTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE04</accession>
    <name>A0A9W8YE04_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE04"/>
    <dbReference type="OrthoDB" id="3801082at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="172"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="211"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="292" mass="28483" checksum="76092890D696A34D" modified="2023-11-08" version="1">MANSSSGGLPGFNHPASTMRSVQATSVAANSTIATSSSIAIASSSAQPLSPSESLSSPTENPASVPTSYDADSTTHLFVTKTEFTTVYPSATPSDAALSAPQASSPESAAPTEAQNTHSAQPTSSNVTAVASSSAAAALPTTSMVPPPVVIITQYTTVAPAPETSSTEAPLAPSSQAAVSSSYAYVSPSPPASSSPASSPAPSSSPPPAVTTPSSSSASSSSSTEAAQSPPPASTETPPSVSGTEPAPPSTSSAGPLIITPIAPSQIFTVTVTTTEKETETATVTVTATVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEE2</accession>
    <name>A0A9W8YEE2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">beta-glucosidase</fullName>
            <ecNumber evidence="10">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_002817</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Glycan metabolism; cellulose degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="10"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEE2"/>
    <dbReference type="OrthoDB" id="416222at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.300:FF:000002">
        <property type="entry name" value="Probable beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1700:FF:000003">
        <property type="entry name" value="Probable beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050288">
        <property type="entry name" value="Cellulose_deg_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019800">
        <property type="entry name" value="Glyco_hydro_3_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715">
        <property type="entry name" value="BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715:SF2">
        <property type="entry name" value="BETA-GLUCOSIDASE F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00775">
        <property type="entry name" value="GLYCOSYL_HYDROL_F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040824107" description="beta-glucosidase" evidence="11">
        <location>
            <begin position="16"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type III-like" evidence="12">
        <location>
            <begin position="774"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4374078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="870" mass="93472" checksum="0813E5EE3310E27F" modified="2023-11-08" version="1">MHLLYFLSISTIVSGAAIEQRDAVPAGYVASPYYPTPHGGWTSDWSESYRKASELVSNMTLAEKTNLTAGTGIFMGRCVGNTGSALRVGIPQLCLQDGPLGVRNTDHNTAFPAGITAGATWDKDLLYRRGVAIGEEFRGKGVNVHLGPSVGALGRKPRGGRNWEGFGADPVLQAFGGALSIEGIQSVGVIATLKHFIANEQEMYRMYSIVKPGISSNVDDRTLHELYLWPFAEGVRSGVGSVMIAYNAVNGSACSQNSYLINGLLKDELGFQGFVMSDWLAQISGVPSSLAGLDMSMPGDNSDIPVVLGTSYWMYEYTRSVLNGSVPVDRLNDAVTRVLAAYFQMGQDQNYPRPNFDTNTQDAVGPLYPAALISPQGVVNEFVDVQADHADVARNVSRDAITLLKNQDNALPLSRNVTLKVFGTDAEKNPDGINSCTDQGCNKGTLGMGWGSGSARYPYMNSPIDGFKARGATYQFFNTDTFPSNSNPSPNDTAIVFVTADSGENYITVENNPGDRTSSNLNLWHNGDTLIKDVAAKYANVIVVVHTVGPVLLEEWHDLPSVKAVLFAHLPGQEAGDSLMEVLFGDVSPSGHLPYTLPKSESDYPASVSLVGYEIGQPQDTFTEGLYVDYRHFHKAGITPRYAFGHGLSYTTFSFSNATITAVMPLTATPPTRPAKGQTPAYSTAIPPASEAYWPANFDRIWRYLYSWLEKSDADAAAKIGNSTTKYPYPEGYSNVQKAGPPAGGAAGGNPALFDIGYNISVTVTNTGNRAGKAVAQLYVQFPSTNTIDTPIIQLRDFEKTSTLAPGASETLNLRVTRKDVSVWDVVSQNWIVPVVDGDYGIWLGDSSDALHLRCGTASGSCAGNQTSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF18</accession>
    <name>A0A9W8YF18_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001524</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF18"/>
    <dbReference type="OrthoDB" id="3432393at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4376241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="773" mass="84365" checksum="192BE36431277662" modified="2023-11-08" version="1">MPITAVIRASSHGPLQQIQIGAADIVATVSAAHSAWGWIGGLTGISNLLGLCKDASELEKLGKLFKHIEPDPGTCRIIGQNGLVEVEMGAAGNDAAFGGSFSSRLIGLTFCALAHNMQTQSAVGMCVQFIVDGLFKKALSTLPGAREAVMTFLVEKSQVIINEGIVRHLPTKFDQAIADIGIQVAGNACNHSVDHPMEAPFILGFFIWLTKANMEPYHTRSAAVARLAASLRIIGYKIGAVQVWRGHGERPKPPRTVVLVTSGSSDTDRYAGPLDRGTRSITMISHYRWNTVGAMLWNAQVQVSECPFETYQSDFEDTDSALMMTLHGFQWAVVDLETAERSQELQAYPIWENESPKRASHIARRLASLVFRESVERIAFLYEPIATESYLAALSPNDRARKVRSTASDSKESRRFFALSISICLAVLAQVGGNNFRAIQHSTTLNLMHDDNLRWLCYEVDRILCGGCRVSQVIRAIATMHCAKEFPNEHNAYVGGQADPSDADEIPTQVVGWKLGRYAVLPNLLFCMDTPISKEILDLHCVDEFIANIPTRRDGSVHCPSVLASSIFFSSTPLEGLEATAPADVEAGPLSHNNSRPVSIGHPDLQPADKEVYISLERSAYIGIYEPDVTLCGRVEGVTIGRVGIQDVLRTLALSWNDGEGYPIKICSTGIDTLHLHDDGSSNIGADTVYNMPVSRYTEENDLTQCPKQNNQAKEKRNVFVQVSGNTPWALLLAGQSPELNRVSFGCANCAVRSGSHVTVDHLDGYRTIIGYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF19</accession>
    <name>A0A9W8YF19_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zinc transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MSC2</name>
        <name evidence="12" type="ORF">N0V83_001092</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Functions as a zinc transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF19"/>
    <dbReference type="OrthoDB" id="78669at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904257">
        <property type="term" value="P:zinc ion import into Golgi lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000014">
        <property type="entry name" value="Cation efflux protein/ zinc transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045316">
        <property type="entry name" value="Msc2-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45755:SF4">
        <property type="entry name" value="ZINC TRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="449"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="471"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="559"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="590"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="629"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="663"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="717"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="748"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="11">
        <location>
            <begin position="561"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="496"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="687"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="818"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="11"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="72"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="502"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="690"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="818"/>
            <end position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU369017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4375815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="934" mass="101109" checksum="86E05A6DB4BEB922" modified="2023-11-08" version="1">MASTYALPIVPTSQTHTHGRSRSQYTPEPSPYSNNSLNGAPPATERGHRHYRSEMNGNGQLHGAVRSPYAEYNDHGHDHDHGHERKASNDSTYTLKPFLNGRPKGRPRGESDLGRSPRKGISSSKYGFSPIQETAPAPPPLSSSWLELPEALTALLLPLPYLFASLAYPRAFGRTTKLPSTLSEAVAESSSAIETHDAMGGSPLLHALALSSATLILVGLIAKVNSSLQPLDRRKAEKGTFDINTAPKRILISILSVLLPFYASMQLGGAKTALVLLTATAAGLAALDRKPGKHSPWDDIQRTLRSRKATSGAFLSGCIADIVSSGNITSTLLGHGALLASILLVPPPLPTTGWSIRTKSENEDSYINQGPARTTLAKPASPLVESFENTLLTIAAGLLLTVITILYSLISYSSPFPSYHAAGFSTLSVASATALIYFALSASLRSQKHIGLGVGCVLTSVVGVLERYNAGVFGIAFPFISAIFVGAVMFDLRSPTPSAPHSHGHSHSGHKHAHDHQHDHKHEHHLHGNHSRLSAFLIARATPGSIIHSVLIEKDSRRIAYFGVLNLAFMLVQFFYGFVSGSLGLLTDSIHMLFDCAGLAVGLAAAVMSKWRPNARFPYGYGKIDTLSGFANGVFLLLVSVEIIFDAFERLWEGHELQRLNELLIVSVLGFLVNIVGLTAFGHAHHGHGHSHDHDHGHDGHDHGHSHSHDNENMQGIFLHILADALGSVAVIISTLLTKYYGWSGWDPIASCIIAILIFLSAIPLVKSSGARLMLSLPNDVEYGIRNTLGELGTLRGVVGYTVPKFWLEDEGAAHAEAHAKEQHDCGHGHKHDHDHDHDHDHSHDHNHSADHSHDHDHDHHHDHDHHEHSHAAAQKQRILGVIHIIAARAADVEDVRERTVQFLKGRGMDVVVHVEKEGEGRCWCGGGNGTKMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF54</accession>
    <name>A0A9W8YF54_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002804</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF54"/>
    <dbReference type="OrthoDB" id="5151921at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="534"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="711"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1015"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1150"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="240"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="538"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="559"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="588"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="613"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="639"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="752"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="786"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="904"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="920"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="980"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1032"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1150"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1174"/>
            <end position="1183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1231" mass="133147" checksum="2C35D357CD0BBD77" modified="2023-11-08" version="1">MSGPFRFNQEEAKSPLDRTASPFSAGQPVSFKTNVNRMKTKKWVQAKKNAYDGDDWGDYDEYDEYGVNNDPQREPEPPAAQRYYAQNEPTRSFTDPSQQAPLAKVRRNSFERGEEQRAFSSTIPHPQQGYGQHYAEPQRQTSGPESDGGFDPQQRRDFSPSAMPTPLQTRISAVPSDMSASPSNTQFPPRKSSIGQGDAPSATSPRPRTGSQSDKPLPFIRPADIYRRVEEERERERASLDSSRPSLDSLSSRPKDEVASPQAEGGRSLQPLETVAERKSEYLPEFNAQVQQNGKQPTQNGPSEPVQQGRPPLEALDNSFWSNGPQLQALVSHAPVVPPSDDQGLRSVVDQAFIRTDDQRSIPPTPVSKDSNSEMSRSNTGSTAGISPIMSRVPSSATSALKNRNQAGGEGSTPVIVEESSETATPVSQSTAAFVPDYTRQVQRKPSPGHSRNVSASSVPRSGLATPTRGDSPARSPVIAPHQDLPEPKTAHLATDITEGMQGGLGGPFPAYAARESDIADAMKDSPVGLAPELTAAERQSQHTFLESHNAQSPIQDVVPRDRSESPSKGRVQALAGKFGDVAHSRRGSTQSNLSRNSVQSWEKSQENSRAASPTKGSPGKPSSPVKDFRPHLPGQWESYLTTVTPSDQGDKDKELGLDEDTISHPLGEVDLTPTTAKRPVTEVDTSNDLDPIAALKDAGAAMVNSIRSTVGLDDNSSDNQQERRANKNFGNVYMPRPLQLDRTESAVSSIPPTPPAKDSPESEYPPPPPLKELKDTIPEPFVRQQRPTLDPQLSTAPSADDQESDRLRKEIVASLSPIRTAATPATDPNHASLQPVSPGANRASSILPSEYDSYWADGEDATPRPSQDVDRTVPEPSPVAAKSPITHIEDPVKPSLLNRFSWEESTTGGQSRASPMVEPTKDAQEEKIPSPAVERAAEEERQQWSEGLPDPYFGPGHTFTVTKPDPLTDAELGNRAVTPPLESALSPASPTHERPRSPGLHVVNTAVDPEAVDLPPRFSAEHSQRPQINQEETHELQQQKEAEVLPAAPVSPVGEFKPSVTHEPTPRSPTTDKPLGAREIATITSTQERIATYNKTRDHWANADHGLESWLASTIDANPELATQEFPLQRPPTGTIRHKHTASLPLLGKLGGSSSQQHHGAEQYNAAGAQVPAGSNSPTTGQGRVASHQMQAKGKDLLHTAGVLSGKGMTSAKGLFAKGKSRFGRDKVDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG64</accession>
    <name>A0A9W8YG64_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycoside hydrolase family 43 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG64"/>
    <dbReference type="OrthoDB" id="19657at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08983">
        <property type="entry name" value="GH43_Bt3655-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050727">
        <property type="entry name" value="GH43_arabinanases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43301">
        <property type="entry name" value="ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43301:SF8">
        <property type="entry name" value="ARABINOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040911023" description="Glycoside hydrolase family 43 protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="36685" checksum="C9A30D526CB633AB" modified="2023-11-08" version="1">MRLLTLLTLLPAALSLPTESLDFSDHGLLARADPSLTGYLGVFFLGDKPSIYFYLSNGNNALSMTALNKGSPVINPTKGTLGVRDPSIISGGGDEKGKKWYIIGTDLNIGKTTWDASQRTGSRGIFVWESTDLLTWTSERLVTVEDSTAGMVWAPSSIFDAERGEYMVYWASKFYPSSDPKHTGSPSSIRIRYAHTKDFKTFSAPKDYIDRSPTNIIDMEFLPLGNTSFARFMKDESAKTVFCEVSTTGLFGTWTRPGGTNAVIASGVEGPAPYWDNLTPGKAHVLLDFYGGDGYRPYESADVAGGKWAASERAGWPKNLRHGSVMPVTGRRWML</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG89</accession>
    <name>A0A9W8YG89_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000355</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG89"/>
    <dbReference type="OrthoDB" id="3800228at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="197" mass="21517" checksum="63ACB2F4890E2DDD" modified="2023-11-08" version="1">MARNDATGVDASAGEIPSNVADIIPALPPCQQWRIPQAELRDIVGHKEAEWSLDQDLIVRAWNRLVPGRWPGTSVAALATFLQENPISTADDVVNSALHFDQWVGIRNTYKTMKDKICVQVDVGHGAVSQQVDGGTGGIRGSNQSAFNTSHILAPRTIGAELTVESGDRRDFLDGQSLTYMPWLGRILHGIRIGRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGN9</accession>
    <name>A0A9W8YGN9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ras- protein rsr1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">RSR1_1</name>
        <name evidence="3" type="ORF">N0V83_001177</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGN9"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="78" mass="8851" checksum="E64F53816CF7BE1B" modified="2023-11-08" version="1">MSQREYHIVVLGSGGVGKSCLTAQFVQNVWIESYDPTIEDSYRKVLDVDGRHVVLEILDTAGTEQFSKRLYPDMKESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH46</accession>
    <name>A0A9W8YH46_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002251</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH46"/>
    <dbReference type="OrthoDB" id="3691966at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="648"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="690"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="713" mass="78084" checksum="ED6EC0CF1DC9BE78" modified="2023-11-08" version="1">METTQQQLSPQLPLSKASPIIQHALAPGPPPDSPDATQTIFLNHPSARISSSPASSPRIVDPPPKRHGSVHPAMSGTMEGGGGPGANFFDDGSSESEWEDSDGDVTQTFSESEGLGLKALMQGAYDGAHGSRGRSRDRGPGTALPASMQLPAHDSGLGTENEHFSSPVENKGTKRAREMSLKRLGKDNVRLVHTRSTSGTPRGSVGSNISEINSPLAELPKPLPNSRRSEAFESLPKSNSFKGRHRRNTSESVIADSIIDAHVRTMRALEALSPSASLSQAQGRSYPHSTTTSSFPKLSSFTADRHIKITPLSTVDRERPAHLPSHFIRTPYPFTAKKEFPKPKSRPRQRGIDGEDGRGSGDFERLDSGYSEQELRKEYDGRKGKHMLGLICSEGEYDLRSRIKRNEDAQGVIRSLAGSGREGKERVVWLSLQKRTWRRRNGEDARNNLVRIDVPSDLTAASPIRYGKKTKSNGGIEFDDKFFAEQLRAGHHELAGNRFARTFSARKLRRIQLGPTNTWSGCPSQDSTKGTAGLLASGEGIDVFSDSKSPFTEDGLMSLYKKPITGKARYTWVHWARRVAAPNDSQRPLAFQHYATTRPSRRRARSLDSPRKEDEKVTLCAYLHDESATVADTITTIQFVHTLSTLRILFALMLMLALSVGAALLWIFLGAAGTGWRLDLSRQRSDRVGSGMAIGILTLLLEGLGFVAWVLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH59</accession>
    <name>A0A9W8YH59_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000754</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH59"/>
    <dbReference type="OrthoDB" id="291007at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040750321" description="EGF-like domain-containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="329"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="348"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="490" mass="50189" checksum="DF348197EC90DCFA" modified="2023-11-08" version="1">MKAFVLGALLLQTLNAQTTDTTIPDATGTETFEPTTPAPLIIPGPDAPSEAELLSSESDAALGAGAGADVTAQVAATASADNLASAVTAVAEGFSDLASALTSVVAEASLPIGTADVILDVSSSAAATPTADDFVVAPAAADVSATAGAGVNAAAAPPQPGVTSDTSTAATATNSVDALADEPVASPDSASGTGDGGFEAAAFDPATGAYLGGDAGTYDGQDYPGDNTGADDTNYDGQDYPDAPYNGNDNDYNDPDCPAFCYDSDYDTNNNAYPEDTPDPVNPARRAVRARQQPASGGFAAFNWPDNDDGPSAAAYNPQDPCPRSCYGPTTTLRPTYTRTRRPRPTRRPWTTWQPATTTTFRPRPPPFPTTMATSPRPAPPYVQSYTSVLTSDVDWTGATLAGVCPKTCDPFNPALNKCDSTTSCSTSGGTNYYCACRAGFMASGWNAKDFSKQFIVPGQPYVYVAPGVACDQPCADPSCGEVLTRPACK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHE0</accession>
    <name>A0A9W8YHE0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phospholipase</fullName>
            <ecNumber evidence="5">3.1.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_001557</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the phospholipase D family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHE0"/>
    <dbReference type="OrthoDB" id="14911at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09141">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000034">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016555">
        <property type="entry name" value="PLipase_D_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF186">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009376">
        <property type="entry name" value="Phospholipase_D_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="7">
        <location>
            <begin position="303"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="7">
        <location>
            <begin position="792"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="426"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="491"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="683"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="464"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="710"/>
            <end position="740"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR009376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4376274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1023" mass="115920" checksum="B72F21EAFEACA7A3" modified="2023-11-08" version="1">MSNRRDDDLAYGDYHGDEGEEGDRGLIGDMGRRFFGGRKENTQQQQSSNQSGGMSFFKKVHDPLHGFVSDLKDAISGERRTKAPGRIWTTRRIGASARHGNETKWYVDGCSYMYAVSLALERAKESIWILDWWLSPELYLRRPPAKNQQYRVDRMLQAAAERGVKVNIIVYKEVTQALTRKLLNPTLPDYLHSLLPSDTDRFTRGLTRVGLDVIFKSLEVWEETDPLIAPPITVSSAHTKHILEALHPNIGVFRHPDHLPDAAVLQSSFLSSLQNMSLSPAKLAQLPGDGLKAIYGAHEGTVMYWAHHEKLCLIDGQIAFMGGLDLCYGRWDTNQHSIADAHPGNIDKIVFPGQDFNNARMLDFQDVTNWENNKLDRTESSRMGWSDVALCLAGPVVQDLRTHFAQRWNFIYDEKYSKKATRYSRLSETSSGAQQGGVYPPPQQRGFDEEDGERGFGGGDRDEEGERGLFGHHDRGGLRDKLHSKAAQFGHEEGSYQYNQQSHAEHSSQRGGVDCQITRSSAKWSHNLATTEHSIQNAYCEVIRNSKHFVYIENQFFITATGDQQKPIKNQIGAAMVERIIRAARNGEKYKMMINIPAVPAFAGDLKLDEALGTRAIMEFQYDSINRGGHSIYEEIARAGINPMDYIRFYNLRSYDRINASGVMREAEQRSGVSYTQAQEGYDAAHDNSRDYQAYRPPPTAEYGVYEMDGSSSRPPAQSQQYPQYDGGPQSQGNPDDYNRYQQEANKIGGRQGLGDGRWDTVSECYMLGGEDIRNVPWEGGAKDEMDAFVSEELYIHSKLLIADDQIVICGSANLNDRSQLGDHDSEIAVIIQDPDTFDTYMDGRPWRASKFASSLRREIFRKHLGLLKPQDMERPDPNYQPVGIPNVYDWGSNEDMQVADPLSDEFQNLWNWRANTNTQAFGKLFHPVPSDQVKNWKEYDEYYSRFFGQDQKSKENKKPSLYKWGHVVAENFSPGEQGVREVKELLSTIKGTLVEMPLLFLKEEDIAKEGMGLNAITEELYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHJ2</accession>
    <name>A0A9W9CHJ2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_010479</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHJ2"/>
    <dbReference type="OrthoDB" id="3735927at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4362386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="136" mass="14851" checksum="68841E705913F64D" modified="2023-11-08" version="1">MANQVAPSTQSLQASEGSLEHRLLELLYPFRDECSTNDAVSLVKRKAQILCGNIAFLIRHNQSRFGKKVYPEDVSIASRNWDGMVNGSGQMGIGIFVIGNGYTHTVLKATVQPGASVLDKLTQVVEGFLEEFVPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHU3</accession>
    <name>A0A9W9CHU3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHU3"/>
    <dbReference type="OrthoDB" id="5304883at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF32">
        <property type="entry name" value="PROTEIN KINASE COMPLEX COMPONENT, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G07660)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="32423" checksum="ED8197A2460FFD23" modified="2023-11-08" version="1">MNPPSAPNPAGDAGVLPEFGNLSLQDAPSPPPNDEDLDVLTMSRETAMKLIVRSVVAIANSAGDVPATPPLSLSRPGTPGGKENHLLRNHRRTASRPATPIPAEKENHQPTELAPPEAGHDEPVTIHDIGHGAQPEAVQRANMARKFFSKSVPKVGVEEYMNRIQKFCPLSTAVWLAAGSYMLRLCVIDRSVPLTYRTMHRLVLACALVAMKALEDHRWPQKRFAAVGGVDEASLSRLELCVEFLLSFDVQIFTPEKLKELTIQLQKAGQAATLTCKLPSSFNLKLSKPRLRQEQVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHY7</accession>
    <name>A0A9W9CHY7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010282</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHY7"/>
    <dbReference type="OrthoDB" id="2988756at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF166">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="53"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4363162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="213" mass="24272" checksum="8EEA4E2AC994E32D" modified="2023-11-08" version="1">MAEDSESTVSMWVCLMLAVALIAARLGLRRWRGQGFTRGDYWCLAYHRSVVGNIWLFTYEISNILTDVLLMALPFSLILSVRIPPMQRLRLLFLFSIGIFLIAVSIMRIIQGRNSRVQSAHTMWASLEILFAVIVAVTPTIYALVRNRKEDTTYPNQSHISMPGRTHPGSVTEIDRYAARVWTELEDSASRTDNTSMEGILVERRYETSDVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIC4</accession>
    <name>A0A9W9CIC4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008854</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIC4"/>
    <dbReference type="OrthoDB" id="5339776at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025040">
        <property type="entry name" value="DUF3984"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13136">
        <property type="entry name" value="DUF3984"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="410"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="459" mass="50140" checksum="75A7D2EE2E69DC84" modified="2023-11-08" version="1">MDTTSTSRASSRSRRSYPNLHNLSLAPLSSKYPLDASTPPSPSDESTHTTPLRTSYIAQKSAPTTPGILSLSQSRSNSRHRLGGKKAYAYDTYFAVDSEGLVRGNDAVVRDVGDIPKAKSTSTLLHHGGRDVHVGFADEMQGGAVGEGGQKRHHMRKRTAPLPLRMPIVRHHTSEANDEWFHRAGLAIAGETRDSKGQGWLVRRESSTSLVNESDGYEQHASHDAGRHIALLSGEHLGDADFPTSFFSPRMSRAGSRITSRVGSRVPSARQSRRGSRVGSRVDLYMSTSKPHSGRESFDELEDRFIEPDFIEAAAEEESDGDEEEVARLARERASGLGGWMDRLIGWTLFSVDEDGEGSSSDDSDGEEDGGRAPPMTTLENMTRDEMKLRREVEAKRRKLEREAIIAASALKGRDRVDGGEESSRPSTGDTQRPGTNEEAGGWQDAAWLLSVASKALLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIS3</accession>
    <name>A0A9W9CIS3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIS3"/>
    <dbReference type="OrthoDB" id="1046782at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359:SF37">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="462"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="640"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="245"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="641"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="827" mass="92670" checksum="21BC60277185A30A" modified="2023-11-08" version="1">MHNSQAENEIPFRINRVTGSASVRLPSQESATPPPHEQSRQQAVPAEFLGTGTWTVSGAEEARDLSASDASRARSHSHTQYDLQAQRHLPHSSETPSNGSVGRENLLLSPTGSDSRRDSLFNTLTISIQSHRIFVSEPSSSNDSEVSSGDELYESCTDFGTTVTPVVEVQDDSTNEGVDLSQDIDADDVSSVNLANESTTSIHQEQSNPNRPYTDSLSLPGRIPTGVSPALSIISDAGHQQQLRQKQSISSKSDQQQRTSQQEDGAVLSSAVSVGLNLQGKIFHALVTKPKNPDFGFVPQEQLYQIVNADSVASQLKTDLKHTHTSEEIAKYANYVCKEPEVLRQGKKKLKSFQKIFATLVMVGMSASISHFLDEDVSDLDLPLAPIRQGGMVISLCRQDSSGNPTTPLRCFNRDLWSFGQLRSFARDQWNMLAPFFSRGENGEVKHYMLQDQHILPFVDAQMAEDDEVYGGFAKVMMVQIHPEHHNFKDKMLCDRGFAIKQLNDSDHEAFTREVNILKKFSGARSHKHIVSLLATYEQHGKFHLIFYRAGGDLFKYWKELEPHPPFHYFNVLWMAEQCAGIADGLMKLHKHITFTIRQKDIVEQNGPNPVGDRRTKVKIVDPIFHVRSDSFSNGVRVRPSSPTWWRDQSQSTSGDRTKHRDVPQNAVLIEEEKFGRHGDINPGNILWFHDSSTSGGTQKGTLKIADFGQAELNSAQSKTRPRDVANTMTYRPPECDIAPRIIRQSYDIWCLGCVYLEFVTWILGGKQLLERFNKRRSAPDRYLNNQKIDTFFEFVKNQDTAELEVGIKSSVTKACNPRTNAVSCCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ12</accession>
    <name>A0A9W9CJ12_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Mitochondrial porin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">POR1</name>
        <name evidence="11" type="ORF">N0V83_008793</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic mitochondrial porin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ12"/>
    <dbReference type="OrthoDB" id="7827681at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008308">
        <property type="term" value="F:voltage-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07306">
        <property type="entry name" value="Porin3_VDAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.10:FF:000012">
        <property type="entry name" value="Voltage-dependent anion-selective channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001925">
        <property type="entry name" value="Porin_Euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743:SF70">
        <property type="entry name" value="GH26960P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743">
        <property type="entry name" value="VOLTAGE-DEPENDENT ANION-SELECTIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00185">
        <property type="entry name" value="EUKARYTPORIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00558">
        <property type="entry name" value="EUKARYOTIC_PORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-0626">Porin</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4365175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="297" mass="31203" checksum="DAD056FADD488286" modified="2023-11-08" version="1">MTEYVKVEKVIPKFPPPAFSDIAKASNDLINKDFYHTAAAALEVKLKAPNGVNFTAKGTSAHDGPVTSSLEGKKTLSNGISITQSWNTANLLNTKVELNDTLASGLKAEVLSNFSPAAGNKGQKVNFHFKQPNFHGRAFVDYSAAGAIGTTADAVVSHEGFVVGGEVGYDVQKAAITKYSAALGYTTPLYNAAITATNSLQVFSASYYQRVNPAVEAGAKATWDSKSGSNVGLELAAKYKLDPASFAKAKINNLGIASLAYNTKVNSGLTFGIGGSFDTQKLNEAGHKLGTSFTFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJB5</accession>
    <name>A0A9W9CJB5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase</fullName>
            <ecNumber evidence="4 14">2.4.1.131</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ALG11</name>
        <name evidence="17" type="ORF">N0V83_008857</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 14">GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes, on the cytoplasmic face of the endoplasmic reticulum, the addition of the fourth and fifth mannose residues to the dolichol-linked oligosaccharide chain, to produce Man(5)GlcNAc(2)-PP-dolichol core oligosaccharide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>an alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + 2 GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + 2 GDP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:29523"/>
            <dbReference type="Rhea" id="RHEA-COMP:19515"/>
            <dbReference type="Rhea" id="RHEA-COMP:19516"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:132511"/>
            <dbReference type="ChEBI" id="CHEBI:132515"/>
            <dbReference type="EC" id="2.4.1.131"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12 14">
            <dbReference type="Rhea" id="RHEA:29524"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.131" evidence="4 14"/>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJB5"/>
    <dbReference type="OrthoDB" id="2276068at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004377">
        <property type="term" value="F:GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03806">
        <property type="entry name" value="GT4_ALG11-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000168">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Alg11), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038013">
        <property type="entry name" value="ALG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031814">
        <property type="entry name" value="ALG11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919:SF1">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15924">
        <property type="entry name" value="ALG11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14 17" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="ALG11 mannosyltransferase N-terminal" evidence="16">
        <location>
            <begin position="96"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="15">
        <location>
            <begin position="346"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF15924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4365239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="557" mass="62548" checksum="C898B688F3639C38" modified="2023-11-08" version="1">MAVLYLLLSLLAPLLVSVIFLPKTLRFVAELVGHHLRRSSRTRRELLLARVAHETKRYESEHKEKSNEDDDWEQITSAAVGSAVNGGRAASEWNGIVGFFHPFWYGFSGTVEEANAGGGGERVLWAAIRATQKRWPEAVCVVYTGDHDVDKVAILKRVKERFNIQLHPPTVHFLYLTTRHWVLASTWPRFTLLGQSLGSLVLAYDAFKLLVPDIFIDTMGYAFSLGLSSFCFPTVPTGAYVHYPTISTDMLDSLQVGGQGVNAGAGAGYRGMAKKKYWELFAKLYCRVGSTIDVVMTNSTWTQSHIKTLWEPYRIKRSKSEDIDVVFPPVAVEEVTEAVEVSEASEKNRGPYLLYIAQFRPEKNHQLILEAFASFLHSNPHIATYNNKAPKLILIGSVRNSHDDAKRVYKLRLLAHELKIEDNVEFICDAPWPQLLEWMSKASVGVNCMWNEHFGIGVVEYQAAGLISVVNDSGGPKLDIVIEIDGKPTGFHATTVEQYAEGFRKALALSPQDTLAMRRRARKSAERFTDRGFAEKWLTNMEKLVALQVERSRSKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJM4</accession>
    <name>A0A9W9CJM4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJM4"/>
    <dbReference type="OrthoDB" id="5280838at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38119:SF1">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38119">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="458" mass="51003" checksum="2FD08FEBE807A344" modified="2023-11-08" version="1">MKKRSRSQSPAAPHYPITPGPLKDPSTGSSLKASSEPLPSVKPYHTPPESFPIFRSGDVYIQSKLAKPSKNWQLQSDVLARHSSWFARSLEQDQKQEDSNKNDQPTSYSYTIEDTGLVRQQVKGDESLHKLLDNESVLGMKVELEETKDSTSTTKEPSTLTTTPASASPPKAPRDLIYNQVFGAFYNIPPSIPINSISAACTHSEQLVELAHNLGCLHLITSHISTALLSYRQILFTAIKDDPARWLLLSLKLQNDNIYTESLIHLAGAHPCWPWPTPTKRTHLPDSTRRLIAKKSSELDQACLEAERSLLLLTIRIGRTSNSSPVQPEDPSHFPTWFVVQSYRDILARELHALESSKSAALKRGTMFRKIWRGGSAYMDYEEMRRLMEQIMPSAVENLSEDLALLKEFAGGIVDDLARNEAMVDVEACGVGWLTCVRVERGSDIPWRNGGGGEKSEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK93</accession>
    <name>A0A9W9CK93_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative phospholipase</fullName>
            <ecNumber evidence="4">3.1.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_007405</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = a 1-O-alkyl-sn-glycero-3-phosphocholine + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17777"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30909"/>
            <dbReference type="ChEBI" id="CHEBI:36707"/>
            <dbReference type="EC" id="3.1.1.47"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the serine esterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.47" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK93"/>
    <dbReference type="OrthoDB" id="2363873at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003847">
        <property type="term" value="F:1-alkyl-2-acetylglycerophosphocholine esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016715">
        <property type="entry name" value="PAF_acetylhydro_eukaryote"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10272:SF11">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10272">
        <property type="entry name" value="PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03403">
        <property type="entry name" value="PAF-AH_p_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018169">
        <property type="entry name" value="PAF_acetylhydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="322"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="349"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR018169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR018169-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4366877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="495" mass="54059" checksum="1E30F4742D8F1FA1" modified="2023-11-08" version="1">MVFQLGHFTWRRVLLFASAVFLFFIFIFSLTPITSPLPPYTGPHEIGILDVETEIQRRVVHDAKFKDTGENAFELNTLAITLYYPSSLPVAPPSSRPWARPWLPQPVSLIGEGYARLAGVPRLSSVFTFALWLLGSSTVIPGVVDAPILSGVEKLLVDESGGASGLGKILKVAEEEHQELKRDEGFGILPCVVFTHGMAGMSQSYSHYLGSIASHGYVVAAVEHRDGSGPGTIIHYPNGKKQKVWHLKLKDLEPNPLMTELELKAAQLAFREAEISETIKLFAQLNAGEAPIVNLKPDSPRESLPGFKNRLNLSAVTVAGHSYGATGAMQALDTAGSNALPINGGIALDPGKESGPLNKNIEVPVLVMQSGEWTEKQTKFYDQGWHFDVVRKIIESVDKGWFMTLTGTAHPSCTDAPLIVPWIMKLVTGTTLDSRVALREYIDTSVQFLKYLQTGEKKRVLASEVTSAAGPLGEPDKRTKIKGLYGADWEIHVTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKJ2</accession>
    <name>A0A9W9CKJ2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mitochondrial heat shock protein Hsp10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hsp10</name>
        <name evidence="5" type="ORF">N0V83_007156</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GroES chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKJ2"/>
    <dbReference type="OrthoDB" id="184876at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.33.40:FF:000002">
        <property type="entry name" value="10 kDa chaperonin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="entry name" value="GroES chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00580">
        <property type="entry name" value="CH10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772:SF0">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00056825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4367572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="103" mass="11158" checksum="558B1030B7E6EEA2" modified="2023-11-08" version="1">MSAIKSIRSIAPLLDRILVQRVKPEAKTATGIFLPETAVKELNEAKVIAVGPGAFDRDGKRVTPSVQPGDKVLIPQFGGSPIKVGEEELSLFRDHELLAKINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKU8</accession>
    <name>A0A9W9CKU8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">superoxide dismutase</fullName>
            <ecNumber evidence="4">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SOD4_1</name>
        <name evidence="10" type="ORF">N0V83_006993</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKU8"/>
    <dbReference type="OrthoDB" id="159229at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000007">
        <property type="entry name" value="Cell surface Cu-only superoxide dismutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053257">
        <property type="entry name" value="Cu-only_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20910">
        <property type="entry name" value="AGAP001623-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20910:SF1">
        <property type="entry name" value="SUPEROXIDE DISMUTASE COPPER_ZINC BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040972630" description="superoxide dismutase" evidence="9">
        <location>
            <begin position="19"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="183"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="183"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4367411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="250" mass="25010" checksum="26FE5E6AF21A6A34" modified="2023-11-08" version="1">MLVQLVSLLAAAASIANAQSSTVTPAPKVTGNPAGASFVGTLPEKAGSALRGSIAATTENDGSGVRFSVSFSGLPAEGGPFMYHIHAKPVPADGNCTGTGAHLDPYMRGEVPTCDASKPETCQTGDLSGKHGNFTSQAFSAQYVDPYLSTSASDPSFFGDLSFVVHLANKTRIGCANFTSMAKPSPSSGGLPKPSEAPRYNSTIATPLPTGGAPISPSASATKPAEFPGAAAKVVAGPAALFALVAALAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKW1</accession>
    <name>A0A9W9CKW1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_006066</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-binding monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKW1"/>
    <dbReference type="OrthoDB" id="74360at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051209">
        <property type="entry name" value="FAD-bind_Monooxygenase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42877:SF7">
        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42877">
        <property type="entry name" value="L-ORNITHINE N(5)-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4368984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="617" mass="68757" checksum="4C7EBACC39CD107C" modified="2023-11-08" version="1">MSAIPNIPSDTPGIHTNGIANGASTASFKLNNVPVENLRPLRVIVIGAGYSGIYCGIRIPERLRNVELAIYEKNGGVGGTWWENRYLGCACDVPSHSYQYTFEPNHSWSSLYAPASEIQAYLERVAKKYSADRFIKLRHEIKACHWDDKTAKWHVTIKDLSTGETIRDQSDVLISARGNLNNPSWPDIEGLNTFKGELMHSATWNESYDFKDKRIGVIGSGSSSIQIVPSLQRLPGTQISTFVRSKTWISPPFGQEFFDNHGFQGSAIPEDLRKRFASDPEYYYKFRLSVEEDGNGIHAVTMKGTPLQLGAKDMFYQHMKKRLESKPEIFEALIPSFSPGCRRLTPGPGYLEALTEPNVNFITTPISHISESAIHTTDGASHEIDALVCATGFQAGAPPPFPVTGTNGLTLTEKWSARPVNYLSHSIAGFPNMFTMLGPNSAIGSGSLTCMIETVGDYIVKAIRKLQKDNIAAMVVKDAREADFSEYVDAYFQGTVFADECRSWYKNKGTGEVVGLWPGSTLHCVEALRSPRWEDFEYVYVDELEESGADDHEEGGLNGGKGRNRRANRLAWLGNGWSVNQLEERHLAWYLYPEFVNQPVAPRPEEKESLGIRPFSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKZ3</accession>
    <name>A0A9W9CKZ3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">RNA-splicing factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CWC25</name>
        <name evidence="10" type="ORF">N0V83_007235</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKZ3"/>
    <dbReference type="OrthoDB" id="21123at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022209">
        <property type="entry name" value="CWC25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051376">
        <property type="entry name" value="CWC25_splicing_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16196">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16196:SF0">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC25 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12542">
        <property type="entry name" value="CWC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="130"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="10"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="182"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="266"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="313"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="336"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4367650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="398" mass="46956" checksum="0444935E8F5A8269" modified="2023-11-08" version="1">MGVWKEEKSALEERKAIDRLKKEREEERAIEELQRLQAESGGKTFTKRVDWMYAGPSGEGADNTERESYLLGKRRIDHLLKSDDTQSLQKGAAVGIDTVGASIADTSRDTHKKVLQDPLLLIQKQKMEMQLKAAKDAAKEAKYVEKRLKEKEREKRHKDKGRDRERSRDRERKHRSRRERSRSRSDDYEDRSREHRSRRGEGSRSRVDDYKDRDRRERKHRRHDRDDRDGRDSRRRRSRTRSRSRSPYRKSHRDEDRKRTRSRSPRIREDRDDRDTYRRRDPMPARHSHDNYKPRRDWGEDHPVQSAPAPSKAPEEPKESAAERLAKMQAAASSLEEQRAERVREQEIKDAEEAEKLKKNMDGGQRLISKVRGQASNMDLGDAIARGRQGGLRIGEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLJ6</accession>
    <name>A0A9W9CLJ6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLJ6"/>
    <dbReference type="OrthoDB" id="1263307at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052897">
        <property type="entry name" value="Sec-Metab_Biosynth_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37017">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37017:SF11">
        <property type="entry name" value="ESTERASE_LIPASE_THIOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="4"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="199" mass="21244" checksum="8CF6DE77097AE243" modified="2023-11-08" version="1">MATLTAKLRDAGYTVHSRQLPAVGNPNPPSDLSADIAAVRALVEEAIGDEGNDVVVVPHSWAGILAGSALTGLGKKEREAAAGGGRKDPYVHAKDANIFYNDLPESEKQHWFSQLQSHSLATFYTPATAASWKEIPTSYLLCEDDQAIPAAGQEAMIAGVKDLGGVIEVTRLKASHSPFLSRPEETVEWIRRAAGEEGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLW9</accession>
    <name>A0A9W9CLW9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLW9"/>
    <dbReference type="OrthoDB" id="1896086at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="189" mass="20617" checksum="3EB2506B0AB77DA6" modified="2023-11-08" version="1">MKRKYLISDESSWVKNQNKDELGNPIRVIWNGAKEEDHRSSGANGYSNPAAPAPAPPTGPTKQLAVLSSFVPGALTLDWLFLEADYEQGVGCREDFLRPFDTVPWDLSGNGAQDGSTFPGGTRELDLTIDGEKCQYQNDGDDAGALWCGKKVIGCKNDSADKDPNDSTADKGNYQCGDYTRQPVFVCPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CME4</accession>
    <name>A0A9W9CME4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">leucine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Leucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">N0V83_005601</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CME4"/>
    <dbReference type="OrthoDB" id="15954at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00812">
        <property type="entry name" value="LeuRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000002">
        <property type="entry name" value="Leucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000003">
        <property type="entry name" value="Leucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000100">
        <property type="entry name" value="probable leucine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002302">
        <property type="entry name" value="Leu-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025709">
        <property type="entry name" value="Leu_tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00396">
        <property type="entry name" value="leuS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740:SF2">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13603">
        <property type="entry name" value="tRNA-synt_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00985">
        <property type="entry name" value="TRNASYNTHLEU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="12">
        <location>
            <begin position="75"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Leucyl-tRNA synthetase editing" evidence="14">
        <location>
            <begin position="264"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="12">
        <location>
            <begin position="474"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="13">
        <location>
            <begin position="801"/>
            <end position="938"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4369837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1022" mass="115272" checksum="FE3E910400019B14" modified="2023-11-08" version="1">MSRLSLHRVGWRRALRRPVNVRPWIGSRWASSSTERKQRAEDTRVETLTAKWQPHWDRLAPQWHDLRQFDRGNAYVLPMFPYPSGSLHLGHLRVYTISDVLARFKHMQGYKVLHPIGWDAFGLPAENAAIERGVHPEKWTLQNIDSMKEQMKEMGGRWNWESEIRTCDPDFYKHTQRIFLMLHERGLAYQAESVVNYDPVDKTVLANEQVDANGCSWRSGAKVEKVMLKQWFLKIKEFQEPLLRDLDALAENGRWPEKVLAMQRNWIGKSEGTKLWFDIKTTDGDMHFNPVDVFTTRADTLFGVQYIALSLGHPIVRQLATGDDSLRAFIERAKEFPPDSKEGYLLPKIVATNPIGECTHMDDASIPVYVAPYVLDDYGSGAVMGVPGHDARDHAFWRQNAGSAPIQVVVSAKHGLTPSPLIPGDKDTPIAERGFVAAHIDGFGNMPSKQAVDQIVETLRRSGKQAEKTTSWRLRDWLISRQRYWGTPIPIIHCGSCGPVPVPDKDLPVELPSLPDSFFEGRKGNPLAEDEHWKKTTCPKCGSAAERETDTMDTFMDSSWYFFRFLDSKNKTALIDPERANNGMPVDLYVGGVEHAILHLLYARFISKFLATTSVWPEGNLAKGEPFKRLITQGMVHGETFTDPENGRFLRPDEVDLTNSSRPIIKASGSIPNVSYEKMSKSKYNGVDPGATIAKYGADATRAHMLFQAPVGDVLEWDEKKITGVQRWLHRVVRLSNASWIPDDQLEKFQVPSHIDAKLPEIFEELSAKSVLRPAQNTPQGPLQSETESALIASITPDNAKLWTRTQQTIASVTKSFSQTYSLNTIVSDLMTLTNTIWDTPHSSTATPYLKWYSTIHLVRMMAPIAPGVAEESWHLLNNLRDSQSSGFEGSPTASRIPQTVFATGFPSVDLDIIPKLNHTRKCVVQVDGKRKFEVDIRKLPESISPKDTEAVSRWVLDQLIETDEGKEWLDRTSGKIWKASQTDQPHPIYQDLPDDWKVIAVNGGALCNLVGPKKPKIAKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMR8</accession>
    <name>A0A9W9CMR8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMR8"/>
    <dbReference type="OrthoDB" id="5388486at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039970">
        <property type="entry name" value="TF_Grauzone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23225:SF2">
        <property type="entry name" value="AT09679P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23225">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="481"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="481"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="502" mass="57481" checksum="E880DA379F07BA78" modified="2023-11-08" version="1">MDDSRLFSWGVDHTNYMQTYKQQVISFSSEDEIYFDDFDQGVVSMQPMQRFMLDQCPLTPGLLNAKIPLQIRPDRHYEQQWPSADCFRNPSPDRTSASGNSSAATQNELHSPHTYHSVLYGSPTEYFQTTLPYPSTEHFHDGSYLSEEAVHGGCTTSLREVEYEHLELEPELEPITEEVDSVDIKQETGCDRDHDMIKVETTSSFKYADSGIGNSVRDAESVQPIDFYEEPASDSDYSPSSSRSGKRRRSSGSNSSSGRTQRRRGHGRKDSQVSSTSTSTKPPKRARGASNASRGMTDMTVQPDERRPFPCPLAGYGCVSNFSSKNEWKRHVSTQHIKLGYWRCDLCQPTTDANDNQSLYYNDFNRKDLFTQHLRRMHAASKDNSSRNQKEFPVTEDNLPDHQTRCFRSLRDPPQQSSCLFCDRTFDGPSSWEERMEHVGRHLEKDRKTSADMFDYNSWNMNQGLEQYLLYEGLIVREKGGWKIGDGKPKRPQETADESEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMW9</accession>
    <name>A0A9W9CMW9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_005171</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMW9"/>
    <dbReference type="OrthoDB" id="24670at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019183">
        <property type="entry name" value="NAA25_NatB_aux_su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09797">
        <property type="entry name" value="NatB_MDM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4370650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="603" mass="68998" checksum="46DFB46C9F23FFF7" modified="2023-11-08" version="1">MSLRGFIRVPMLSDCQEYWEHHSGLPVCFTDLSGFVQGLPLEDRKAFHKFITDRTRDMITQQGIDEVCTSVDHKTWTSCPNMEQDTFKQWQQAEQNVLKFDYLLTVSLPEVPTYKALENFIIKVTAFWSEFPDSPDAGFLAIYALLDLHHKIVRHQEGKVGFEMTTNARVLLQATMLARHMVARDKNKQNRALALLATRLHLNMGLGKCAFQLFSHTKCKEMLLDTLSPYVLSRISMAHPFDVGGYQGFSADYELAKVIGTIERMEQKTDSYLFTDLQSFVWDQAVDALDLKRKLNSSLTKHICITERRRIARLIGESTDDLPSLNFKDNVDRSVFPSFESTASDGPLSLIMPRGIPNKLWLAEEHCFWETASRVLYREGRLADSEPWESKGLTTKEDFEPVLKTASEKITKDVWVYINVFVGEMSNNGVTDSQAKKHYEHIGNKCIQSIHVIRKAMEKLRMPGSTGLKPEDEPTMFHENMLICCYTNLEMLRALNKLIDHLREKVFNAKSTHGMKKHMPKNWIADLASETHTCYESIRDVAQSYIHLIRRRGEAAIKAQVRWGYTGSALEALLAPGDVDYYASEYVESALEAWNGVMKVKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CND4</accession>
    <name>A0A9W9CND4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">VPS8</name>
        <name evidence="4" type="ORF">N0V83_004306</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="289913at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059070">
        <property type="entry name" value="TPR_VPS8_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025941">
        <property type="entry name" value="Vps8_central_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 8 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23410">
        <property type="entry name" value="Beta-prop_VPS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12816">
        <property type="entry name" value="TPR_Vps8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25066">
        <property type="entry name" value="TPR_VPS8_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 8 central" evidence="2">
        <location>
            <begin position="727"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="domain" description="VPS8-like TPR-like repeats" evidence="3">
        <location>
            <begin position="1265"/>
            <end position="1481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1388"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1522"/>
            <end position="1556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="113"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4372531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1607" mass="176988" checksum="4DEB0CAF3DFC673F" modified="2023-11-08" version="1">MSSDSGESHSSAHQDHGDEIDTEVGAKQGPTERAEVDEASATSGLVTAEADEDEVESIGSGPNGLTFARTRRSFPTHEDEETASELSIRPKIERPGSPESTSTPDDTPSIQGSGLSSPGSSVPVSHSSLRPHRPASLQPFERRFSARLSPSPLASPRGASPAFLSAHSRQSSVSSNLLFRQLSDGDGADTPQAPWDVVRWTKLKKITGQVFSEVGKRNFGRPTCLNVTVSLVIGTSKGFILVFDYQQVLKSIIGPGTKAVECGAITALAISADHSTVAGGHATGHIFTWELAKPAKPFLHIPPLERATLDDRKSDGHVSGVGILHLGFLGTRHTALASADDGGMAFSHLATRGLGAIARSVKITRILGRYPPSAKSLEKPRKPSSVLAFSPLPLGNVEQPTDDMGLTALLTPYLLVIVSTTPVAQTQHKAPRPKEVAPHSALSGCLAWFPAVKLKNPSNDSSKAVSKTKLVYCWSNVLTILDVDSIVAPPTEKEKPTELRFRPRSRWKAEEAVVAVQWLSRSVLGVLTISQRLVILEDNTLRMTDSFDLLQKHIYHSDLFSRQLRPVIEQLDEQDVSMHGVVADAFYMSFRAYKGRLFLLGFNDVSIGTLSNWADRLMALMEDGDFIAAIGLATSYYVGDADKLTVGLPEDDKARHTLVQEKLLEMITASLKYTFSRDERNNDEGRDQQLKELAEVVFTGLFSMKEFGFLFEDVYDAFEEASAEKAFFETLEPYILDDEITAVPPNVLKDLITFYASANRASRLEEMICRLSTDTMDINQVTTLCQQYVLYDALIYVWTRAIGDYITPLTNILELIKLVDFDVDETENIYLTSAKKIFPYLAYTFTGRIYPGGAPMDDEQAHSAKKDMYRFVFSGKSLQWPPGSGEIILTRTDAGPEPPFPYLQLILEFDASSFMSMLNEAFEDSFLNGEQETTNGTQVNGIQQGSTLTPTRQYIINILFGVMTTENFDLEDILYFYMFVARNLPKYPQHIMLRGTLSHQVLVGLCHCPTEIMKEDCQLSVEYLLSMYHPPDPQSLIPLFEKAGFHRVLKSVYRGEKQYAKLLETYLDDSEDEEAVFGCISDVLRPSKGLIKKQVSDVKRVIVNRATELVAIDASQTAITLKQYAPDLLEPVLEAIEDDSDAQYRYLAALIEPEQSHAQAGDLASINEDLPSGFIETYVQLMCTYNSSHVADFVTMLKSSNLRLDPILPALEQSGVIDAAVILMARDGLVKDAMDRLVGHLGTLETALTGLITAAAQSPDVANTEEAVHDLLEDIQKYIKVGIWLCQGQTRSKKRTPESVDDRRKSAYGEIREGDLALDELLWLDLVDTSVRLIRDVSDAVANLDAAVTASNLDQSDIIDSARIVSTLRSSIQQTFSALLASTTIPPATQATSNTTSKSVTSRPPLRHTQSQVQSNPSFLRILRCFLTRATHTYSPSLGHLRSVLAEVFAAYTFEETILSLANRFLDKESFEHVHEITELRKRGWRPRGQTCEGCKKRAWGPGAGAANGLWEAWEKREAERSRKRIEDANGVEDESGGKGKGKRPSEAEIDVDAEEQSSDTAGALVLFACRHLWHKKCVEKASVSEGSGAAEGTKTQQGRLKCPLCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNM2</accession>
    <name>A0A9W9CNM2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_003753</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>an acyl-CoA + acetyl-CoA = a 3-oxoacyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:21564"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="2.3.1.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4371980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNM2"/>
    <dbReference type="OrthoDB" id="5404651at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000010">
        <property type="entry name" value="Acetyl-CoA acetyltransferase (Thiolase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050215">
        <property type="entry name" value="Thiolase-like_sf_Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853">
        <property type="entry name" value="3-KETOACYL-COA THIOLASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853:SF9">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="10">
        <location>
            <begin position="19"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="11">
        <location>
            <begin position="284"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="8">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="363"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4371980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="408" mass="43806" checksum="59163D530CA4A50C" modified="2023-11-08" version="1">MATFTRAARNVLQKSPNDVVLLSAVRSPINRSFKGGFKDAHPEDILMPVMQEAVRRAQIEPKDVNDALIGNVLAELGFAKTGRMALNHAGFPNSTTFHTVNRQCSSSLQAITHISHSIMVGQIDVGLAGGVECMSRNYGSRGVPTDVSPALLGSTVKDARDCLMPMGITSENVAERYKVDRKSQDEYAVQSHGRASRAQKEGRFDWETVPVIAQRMDEATGQPSGFEVTKDDGIRHGLTFEKVSTLKPVFGENGKSTAGNSSQISDGASSTILARRSWAEERGLKPLGRFVGTQVRGCAPDEMGIGPIYAIPALLKWTGVELKDVDVIELNEAFASQTIACIRELGLDEEKVNPNGGAIALGHPTGATGARQTATLFGELRRQDKEIGIVSMCASTGHGVASLFVRES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPA2</accession>
    <name>A0A9W9CPA2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Autophagy-related protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_003283</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPA2"/>
    <dbReference type="OrthoDB" id="1668230at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF68">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATG1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="87"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="29"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4372992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="438" mass="47078" checksum="13B7D4E9863AA701" modified="2023-11-08" version="1">MGADDPRTLHDSGALTPPITPTCSSHAKSTSISSDFLSTLSSLRRGDSTSSRTSTRASMDMDRKPGGAEPIYFPHHLTDYAIMVTAKGKKIIIGEGLWSDVYLARPSVPQHKDQLELPAPAGTTTPPTTPIHSRKSSLEVHQLPASPSSYAIKVPASTSAKKVIAAEGKILSYLSRFPNAERHVIAFYGLDTRTGALVLKAMDGTLETWIQQELNVLDETSRAQKLATIFPAIATSLLDSLLWMQDKDCIHADIKPSNILVSNTSSTTIPTTVFADFSSSVVKTQSSDDTKTIETSPIGAGTWEYIDPSLLSTRSPATPSASTDLWSLAITLLFLVIGNSPFDAFKHNKFQQREMIKAGAPLQCLGYSDDGIKSIKRLSELSGALGFDVQGWFAKVLVKGTEGGGIGKWKEEIVNAVAAAAAAAAARGWRRCEWLCVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPD1</accession>
    <name>A0A9W9CPD1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_003036</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPD1"/>
    <dbReference type="OrthoDB" id="2122304at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.550">
        <property type="entry name" value="Membrane associated eicosanoid/glutathione metabolism-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023352">
        <property type="entry name" value="MAPEG-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001129">
        <property type="entry name" value="Membr-assoc_MAPEG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35371:SF1">
        <property type="entry name" value="BLR7753 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35371">
        <property type="entry name" value="INNER MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01124">
        <property type="entry name" value="MAPEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161084">
        <property type="entry name" value="MAPEG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4374295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="133" mass="14801" checksum="373A0DF521DB4D6F" modified="2023-11-08" version="1">MSVIKKANNNKWNNHNPRSTETDERYRKGVPAEVYAKYERAEAAHKNGLGKEPRLQNAPFFVGAVLAGNYAGLNSSTLNLITGAYLGLRVLYNVLYINTTTQKASYLRTFTWLSSTILLIGTYIKAGNILSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPE4</accession>
    <name>A0A9W9CPE4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001857</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPE4"/>
    <dbReference type="OrthoDB" id="3796140at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="300" mass="35032" checksum="A395C55C9485CD64" modified="2023-11-08" version="1">MASQRQKRLSQERKKKKKKWDSGKSHVKINTATQLRTLKASERKKLERQREPLRQMMPWVHDPSYRSHERVYYRATFLGLPSELRQKILYQSVHVASLGPGVDAEIEMRRDTVRTQPFKDDRHGMLCDGQLSMLEMENWDWADPVVALPTLLNARIGSLCTVAPLIRLDMQYVRKLWQKDLDEHIARELQQKQKAQAAAHAVIAEAEGVTHYSELLRRTQKQREPNEVKGRVSRQQPGVRRRPYKCWYCTKRHKDGDPVCPLARSDPEAWEKMTKKIGGWRGKLSESSGKLKNTKIVFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPM7</accession>
    <name>A0A9W9CPM7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_002586</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CENP-O/MCM21 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPM7"/>
    <dbReference type="OrthoDB" id="1103324at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018464">
        <property type="entry name" value="CENP-O"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050364">
        <property type="entry name" value="Cytochrome_P450_fung"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46300:SF2">
        <property type="entry name" value="CYTOCHROME P450 MONOOXYGENASE ALNH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46300">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09496">
        <property type="entry name" value="CENP-O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4373847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="736" mass="83075" checksum="191258D31444733F" modified="2023-11-08" version="1">METMETIERLDTDINDLHARIATLRAHHANLSSILLSQPHLAARLQRVNLRDKSVENAQRIIKQQSKRNIENIYRACVGVTAYKVQDPDPHAINNGNILGVAIDVSIGSKFIETYHVLLNVKESEGKKLLRIHKHTIPPCIPLQQLASKWLPTTNTSSKDIDNAADPEQDLVRFGRSLRKELVAWHMRVQAVEDLRKEAGLPDTRRERNEQDQVGIESTGRILNAFVSDDDEESSDVDEAGDEDDGIVRILEIEPDAAVRQISITWSDGRTAVMNITKDGRVEKAVCRTRGGGRDEARSRKAIGPLGGLGMPRPSTVSLIKSNRVMSGALATGFGRLYGASHVDPMSIPCQCNELARTSVNLDEFSANAILWFKEWSDKYGRVFSLKIGAGCLPGHGACKTAPIDMVSVRLTLWQINPAVEQGTYLPIEQFPILKYIPERWAPSKQRAKVCYTEVTKIWNEARSRVATRRSNDDERESLCDRLLSGSVKCDIPLNDDQISNFLGSAHQGAADTTMSAILTNILFLAKYPWVQEKARAELDRVCGVERMPVWEDFKDLPYINCIIKEGLRLRPVVAVGVPHRVSRDDWYDGMLIPKDAAIFIPGYALHFTHYDEPEMYNPDRYLNHPRLAMDYAGSPDYENRDHYAYGAGRRICVGIHLAERTQWRTMARILWAFNIEPKMDSNTGKPADLDVTAYADGFIRCPLPFQVNFVPRSEQHVKVVETDFRNIKEFLKTWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR53</accession>
    <name>A0A9W9CR53_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Bifunctional purine biosynthesis protein PurH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">HGT20</name>
        <name evidence="10" type="ORF">N0V83_001354</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR53"/>
    <dbReference type="OrthoDB" id="4540492at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015149">
        <property type="term" value="F:hexose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF8">
        <property type="entry name" value="FACILITATED GLUCOSE TRANSPORTER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="293"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="357"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="384"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="451"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="17"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4376073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="489" mass="52249" checksum="D0B843D145F36917" modified="2023-11-08" version="1">MDPRSWFRHLTGYFVYILLIATLGPLLFGFHLSELNAPEDVIRCKRKSIKSTTAGPNLPQCIEMTPTEWGVVGSMYTLGGLIGALSAAPLAGRYGRLRAMLITTIFFAVGPLFEALSPNIGVMAFGRLVSGVGAGASVVIVPLYISEIAPPAEKGFFGAFTQIMCNVGILITQLLGYFLSHDSYWRIILAIGGVIGVVQAAGLLLSVESPKYLAEQGNISLAKKTLRKIRGEDADIDDEMNSWGVAGSGDIHEEEQTLLNNGDEPSESRPKQSSKEDTLSIMQVFRHPDYQKAAIAVIMVMLAQQFSGINSIVMYGVSLLAGLLESNSALLNLAVSALNIVVTAGCAPLADKLGRKTCLLSSLAGMGISSLLLAVGIIKSVPVLSAVAVLLFVSSFAVGLGPVPFILASELVGPDAVDATQSIALGANWIATFIVAQFFPLASAKLHGHVYFIFAALSAFFFAFITWYVPETKGKKNADEVWGRERRVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRB1</accession>
    <name>A0A9W9CRB1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Autophagy-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ATG22_1</name>
        <name evidence="14" type="ORF">N0V83_001371</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Vacuolar effluxer which mediate the efflux of amino acids resulting from autophagic degradation. The release of autophagic amino acids allows the maintenance of protein synthesis and viability during nitrogen starvation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Vacuole membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ATG22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRB1"/>
    <dbReference type="OrthoDB" id="192733at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032974">
        <property type="term" value="P:amino acid transmembrane export from vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17483">
        <property type="entry name" value="MFS_Atg22_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044738">
        <property type="entry name" value="Atg22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024671">
        <property type="entry name" value="Atg22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050495">
        <property type="entry name" value="ATG22/LtaA_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23519">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23519:SF3">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 22-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11700">
        <property type="entry name" value="ATG22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 12" id="KW-0072">Autophagy</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <keyword evidence="4 12" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="317"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="394"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="424"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="460"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="498"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="532"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="561"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="233"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4376090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="611" mass="67178" checksum="1D848283D7E87555" modified="2023-11-08" version="1">MSFVVDDNDHDEDEEEIAIHPPARYEGEDTSPTTQRELNGWYAYPIAAEVFAVVAVGMFLPTPPPKNFPSAISANERIGAFLPVVLEQLARENGVFFSDRSKPCVDVSSGRVRMRAENGNGEKESEQCMITFMGKEMSTSSFAMYTFSAAVLVQACTLVCFSSFADHGPYRKKMLLTFAYIGSVVSALFIFISPSVYYLAPILVIIGVTSLGCSFVLLNAFLPLLVANHPDDSHDNLRSSDSSSQVELGRLNPQNGSTAAAHKFDPDKLPRDLERSAQISSKGVGYGYISAVFVEIISILVLYLFSKTEAQKNNPSLSIRVILLLVGIWWAVFTIPTLLWLRVRPGPPLPTQQLFFTRSKPPSSKMRTFLFYTSFSLRSFWRTLRRAIRLRQTLLFLVSWFLLSDAVATISGTAVLFARTELHMGTIAIALLSITSIGSGVVGAFAWPRVQRHFALQPKTVLLCCVAGMELIPLYGLLGYIPLFKSLGFIGLQQAWEIYPIAVLHGIVMGGISSYARSVYAPLIPEGSEAAFFALYAVTDKGSSAFGPALVGWIVDRAGTIRPAFIFLAVLVLLPAPLLWWLNVEKGRDDARAMADGDGRGRGAYERVSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRI0</accession>
    <name>A0A9W9CRI0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetrahydroxynaphthalene reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001583</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRI0"/>
    <dbReference type="OrthoDB" id="47007at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43639">
        <property type="entry name" value="OXIDOREDUCTASE, SHORT-CHAIN DEHYDROGENASE/REDUCTASE FAMILY (AFU_ORTHOLOGUE AFUA_5G02870)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43639:SF1">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4376300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="28345" checksum="0A4D236DA34E6579" modified="2023-11-08" version="1">MVINVPTASLEGKVALITGAGRGIGRGCAIELGKRGASVIVNYVSSEGPAKEVCQIIEGFNNGAKAVAIQADVSKVSEIHRLFEEAKAAFGKIDIVMSNSGTESWDVTEEISEEKYDHVFNLNARAQFFVGQTAYKHIEDNGRIILMSSIAAGLLGVKDHALYNASKMAVIGFIKAFATDFGKRGVTVNGVAPGGIKSDMFTQNAWHYIPGGTPEWPAEKIESLMASHCPLGRCAVPEDVARVVAFLASDDGGWVNGQVLTISGGSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXA1</accession>
    <name>A0A9W8XXA1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010999</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXA1"/>
    <dbReference type="OrthoDB" id="3856336at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="266"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="501" mass="53196" checksum="1F400668C8DA7CA7" modified="2023-11-08" version="1">MSPPTVVSAFMSLQPLEPVFVFPSDADAALFQSRCKQGRILPTSRQNWVYLPLPEGLQRVRTARRGDVAFDFDSEKHAGEFNKTVKSLGAIYASPKGDHGWEKVVYLGKEKVEGAVEQVARLPRTLGLTRQPSTSASAGRGPPSLGAYRAGVALSLAAGPGAYPAGFAPPTAAGSGAYPAGFGPRRAPGPGEFPAGFGPRDVRAPGPGEYPPGFGPSVAPRPQRRIPSRASERATPRPSLLAGLTSASTTNVGNTTRLPPPPPGFSSASTTTGQGSGNSIRPPPLSLLEQYNQEVLAEIMANRMPVTTLVPVLEKTTYKDEDDTKAAEKTLPYIRVQRAVLRYLEFHASDTLSPITARRWTQDINPETKIPKAEQALSLCYYMREQSFDTYAKIADGFVSLYLVNQSAVPGSGVHTAANDILYWKQDFVTNHVEYAYPDGVGASGGGGGGVMRATPYPFRRNALPGPALALTDDISEVSLGIGGLGAATMASLRSGDLDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYC3</accession>
    <name>A0A9W8XYC3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010429</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYC3"/>
    <dbReference type="OrthoDB" id="3795630at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040800544" evidence="1">
        <location>
            <begin position="22"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="176" mass="18290" checksum="105279981CEF914A" modified="2023-11-08" version="1">MFGKTGLLFLAAAAGLRVAQADFMVYTEPPIPTSAIPSFANPSDAASWTTSVFLNANFAYARFTSSLGSSYEQSSSSAASEVAAFVSTASNYSIPAEVTESGTTTFYSKPDWYTALPSGVRAFKEQQVSDQFSIVRSVIDARATTASSTAGAAPTARAWVDAKFGVMAAAAAAAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ06</accession>
    <name>A0A9W8XZ06_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">gamma-glutamylcyclotransferase</fullName>
            <ecNumber evidence="1">4.3.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_010110</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.9" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ06"/>
    <dbReference type="OrthoDB" id="2924818at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003839">
        <property type="term" value="F:gamma-glutamylcyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017939">
        <property type="entry name" value="G-Glutamylcylcotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12935">
        <property type="entry name" value="GAMMA-GLUTAMYLCYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12935:SF0">
        <property type="entry name" value="GAMMA-GLUTAMYLCYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="115"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR617939-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR617939-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4362993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="237" mass="27034" checksum="DC16D0AB12A67611" modified="2023-11-08" version="1">MAAAQTPQPTIYFGYGSNLWLHQMRIRCPTSTYLGIARLNNYKWLINDRGYANVVEVPKSQRHHPQKDNTTTGKYSNVVFGLVYTLQPSDEQRLDKNEGVPIAYTKELLPCDFWSSSPSSSPSTTSPHHHHKVDTSKPPTTAGREMLVYIDRERVTPDEPKEEYIYRMNKGIADAVDMGVPERYVEEVMRKFIPEPVGEGKGERDDKTKSMEDLRKSVEEFAARQAASFRDESGVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ94</accession>
    <name>A0A9W8XZ94_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DUF914-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_009927</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC35F solute transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ94"/>
    <dbReference type="OrthoDB" id="429955at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009262">
        <property type="entry name" value="SLC35_F1/F2/F6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052221">
        <property type="entry name" value="SLC35F_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14233">
        <property type="entry name" value="DUF914-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14233:SF4">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06027">
        <property type="entry name" value="SLC35F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="361"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="396"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4363631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="417" mass="46149" checksum="6708194B2DF2ABB3" modified="2023-11-08" version="1">MNQIQAYPTSGVVIPNKGDDFGTAGAGDGVAPAIDKTGTGTYVSSENESHRATNAPAAAQGNSEVLEQDAQTKGRWFQYVKTKQFWITLLLGQVLAICITSTNTLSTLLANEGTSIPAFQSFFNYVLLNIIYTTYTIYKYGFKKWCRLLLVDGWRFFILAFFDVEGNYFVVLAYRYTTILSAQLINFWAIAVVVFISFLFLRVRYHYTQIFGILLCIGGLGVIFGSDHITGANNYGATDQVKGDLFALLGATFYGLSNVFEEWLVSERPLYEVVGQLAFWGMLINGTQAGIFDRAAFRSATWNSKVGGYLTGYTLILTLFYSLAPVLFRLSSAAFFNISLLTGSFWGVAIGVKVFHLSIHWMYPIAFVLIIVGQVIYFLRQSAVGEAAKPWLGSRQEKGHAGLGTAKRRAERPEAIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZA4</accession>
    <name>A0A9W8XZA4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009914</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZA4"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040775917" evidence="2">
        <location>
            <begin position="21"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="234" mass="27200" checksum="38033D9C7E7CD029" modified="2023-11-08" version="1">MDKLRSLLSLILHSTPLTTGMGSVYVIIMSWSENPMKPPFKLNAKSFDDAQQLLRNCTNTKLYDAQKDVNRPPDSSLGWDSILEFNEQREDEGHVHNWGWYKHREQAEDVIEALIEVTKHRRTVVRFSTTHYHKTEKVYMCSYGVNEGYQGVGDAVLEQHEGMEMMTRRMITKTVYYWWQIEKVEMPLLKVDEDVAKAGGHREEDDGDTDDEEFEEDEDTDDEEFAEAMGLRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0A7</accession>
    <name>A0A9W8Y0A7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009946</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0A7"/>
    <dbReference type="OrthoDB" id="5121585at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4363650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="447" mass="49487" checksum="68BE71349F43CE0E" modified="2023-11-08" version="1">MLIDHEAVPFINSITVTPLLPLYTVEKGIRKCQFNSNGMFQNPLSAFKTLVSFYCLHPDVPKYISDGLPAKLLQILLLTTYSGIGGYTDDYQGWVKYGMEFLRRQHFRYETLGGAYSYCEDPNDNGITTTCYLEHWLAHYYALLDETNVDRQRRVNCYDLATLGQVMLAVGLDASLDWISRSNNPFFEDGKTDPLWDPNPLWTEEHGKYRSGFRNHAFLSVTDGSAQNIEQVIDATCGPQIATLTLEEYVEAAIDHEPSKTLKFTETSGGHKAGNLISTDGDATKIKSGDGVTRISSPVDVQQYPGNKPTSGLLYAINTAASSGGKFPAGKLIGPSVHTDEMVLNINWMFTPEKDTNVPSIDMTVYSSKGNQLNLEYINRKARFDDADNDMTDIKTLYGATELSKTVVKGVVQLEPGANESFALWQQGLTGANDEALWMIAMATALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0J7</accession>
    <name>A0A9W8Y0J7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009150</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0J7"/>
    <dbReference type="OrthoDB" id="20734at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018828">
        <property type="entry name" value="RRG7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28133">
        <property type="entry name" value="REQUIRED FOR RESPIRATORY GROWTH PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28133:SF1">
        <property type="entry name" value="REQUIRED FOR RESPIRATORY GROWTH PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10356">
        <property type="entry name" value="RRG7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="297"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="311"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="326"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="368"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="384" mass="42146" checksum="A87A097D6E4CB786" modified="2023-11-08" version="1">MRPSLSPPWLTQRALLPWLPRAAPHPRVPRRIATTSAANAAPVDIPKLIVSPGSLHHNSLPSFLEYAKRTKLAPERTVYVGTHYEYTTALALMRLGFSLLRVGRKQDAGIDLIGHWVLAPLREPLPVIIQCKARKISINPAHVRELEGSFQGIPADWRKKDVLGLFVTTKKATKGVLEALGQSRWPMGFVLVSRDGTVQQFVWNRAASERGLEGVGVTVRYTPRALAADAKEEVEEVEVVKIPRKGTKKRSARPAISGTRKDIQMTWMGSPIFPDRDNLDQETVKLMRIIEPDACINEIKPSPGRPRGRPKGSTNVPKTTTVVVKTPRKPGRPKGSKTLKSAKVPSPRGGQVPKAPRKPPAKEAGIRGRPKGSKNKPKPAVDAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0L5</accession>
    <name>A0A9W8Y0L5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nuclear speckle splicing regulatory protein 1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_009672</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NSRP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0L5"/>
    <dbReference type="OrthoDB" id="446635at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053246">
        <property type="entry name" value="NS_splicing_regulatory_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018612">
        <property type="entry name" value="NSRP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47845">
        <property type="entry name" value="NUCLEAR SPECKLE SPLICING REGULATORY PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47845:SF1">
        <property type="entry name" value="NUCLEAR SPECKLE SPLICING REGULATORY PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09745">
        <property type="entry name" value="NSRP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nuclear speckle splicing regulatory protein 1 N-terminal" evidence="5">
        <location>
            <begin position="103"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="14"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="67"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="299"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="151"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="329"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="350"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="369"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4363379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="401" mass="44297" checksum="DC0C2DD3C0D79CDB" modified="2023-11-08" version="1">MSFKFGLKVKNKAANPLANPAAKKGLGKKKPLFDDDDEDVKGKGKAADGAQEIGELNFEDILPLAIRIPKKGQPPAPPNRKPKALEDDPTIGYSASTQESERWVKEAMEADASIYDYDAAYDAIHAASAAKKAAAQEESAERKPKYMAQLLASAELRKKDELRARDKLLQREREAEGDEFADKEKFVTGAYKAQQEEARKAEEEEKLRQEAEEAKKKKLGMLGFHKQMLMEQEQRHQEAVEAAAKALKEGIKPPLVEEGPEKTNAELIEEARKQGRKVILTEDGQVADNFSLLSAGLNIISKPKPAPKPADPSSSISKPQLHQQAGGTKSERNAQRERQTQMIAEQIELANKRKADQEAEEQARLLHASKSQKTATDISSAKERYLQRKREAAAAKATAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0Q3</accession>
    <name>A0A9W8Y0Q3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_009500</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0Q3"/>
    <dbReference type="OrthoDB" id="2544694at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF44">
        <property type="entry name" value="SUGAR TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14610)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="382"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="418"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="18"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4364045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="542" mass="59893" checksum="A54CA1254CED2334" modified="2023-11-08" version="1">MSFQPWRRVPGRVLMVLLNAFIFVALIFEGYNQGVLGTVFGTPGFIDMANIGADGVVTNSTKQGGLASAYYFGAMFGAFIGGWIGDKYGRRRGVFAGAWQALIGGALQAGSVNSNMFICARVLTGLGMGFIMAIVPPWVSELSQAHDRGSNFSFIFVANFLGITIANWINFGVRTSPIEFRWRFPLGFMCVPVLIVALTVLLLPESPRWLMARDRRDEAVEIICKVRGDLQPNDPSIQGELENLHAIVVASKHPRNSYINLFLGGRHSGVLHLGRRAILGFALQQIQQWCGIVAIVTWSSRLFSLAGFDDFKSLWLAGLINTLGIFGTASAGFIIDRIGRRISLLVSFVIQGISLFVVAALIKVSEDYADSDIKKSESFGQGAASFVFIYLWFFCMFNIVPTWLYGTEIWPQDVRAKGYSFTILGWAIGCGMTTFLIPIMLSKIGWYTFIFFGIMNALVLPLIYFFYVEPAEHSLEEVNLLFTSDSPFVRPNVAEYEKRVAAAGGNSAVAARRLMDEVDNRAIPEEKLSEGSDLRVEDVKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y117</accession>
    <name>A0A9W8Y117_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Serine O-succinyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.31</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cys2</name>
        <name evidence="4" type="ORF">N0V83_009885</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. MetX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.31" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y117"/>
    <dbReference type="OrthoDB" id="444135at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004414">
        <property type="term" value="F:homoserine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009001">
        <property type="term" value="F:serine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009092">
        <property type="term" value="P:homoserine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00296">
        <property type="entry name" value="MetX_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008220">
        <property type="entry name" value="HAT_MetX-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01392">
        <property type="entry name" value="homoserO_Ac_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001209">
        <property type="entry name" value="PRK00175.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268">
        <property type="entry name" value="HOMOSERINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268:SF16">
        <property type="entry name" value="SERINE O-SUCCINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000443">
        <property type="entry name" value="Homoser_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="140"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4363589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="529" mass="57991" checksum="DDA0A3AC978E166C" modified="2023-11-08" version="1">MSVPRRLFAVARASRTSSSVQCHAKQSTQPSVLFARQQSSQATRKIHQQAPRKAQHAAAVAQDVESTNPALSFPCLDAHESRSASLSKRSLAGPEPSYTSGHTLNFHSRQPILLDWGGILPEFNVAYETWGNLNPDKSNAILLHTGLSASSHAHSTETNTKPGWWEKFIGPGKPLDTDKYFVICTNVIGGCYGSTGPSSIDPANGERYATRFPILTMEDMVRAQFRLLDNLGIQKLYASVGSSMGGMQSLAAGTLFPERVGRIVSISGCARSHPYSIAMRHTQRQVLMMDPNWARGFYYDGIPPHGGMKLAREIATVTYRSGPEWEQRFGRRRADPSRPPALCPDFLIETYLDHAGEKWCLEYDPNSLLYVSKAMDLFDLGQEHRERINAVRKANSGKLQAYLDGNAIETDTSSEVCSLTLPDKPYEEKEQAADLDAEAMAAQADGSEPPADLVAGLKPLAHTPALVLGVASDILFPAWQQREIATTLKRTGNKNVTHIELGEEKSLFGHDTFLLDLQNVGGAVKNFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y139</accession>
    <name>A0A9W8Y139_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">TEP1</name>
        <name evidence="6" type="ORF">N0V83_009041</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.67" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y139"/>
    <dbReference type="OrthoDB" id="16692at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016314">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043491">
        <property type="term" value="P:phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051896">
        <property type="term" value="P:regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14497">
        <property type="entry name" value="PTP_PTEN-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051281">
        <property type="entry name" value="Dual-spec_lipid-protein_phosph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305">
        <property type="entry name" value="PHOSPHATASE WITH HOMOLOGY TO TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305:SF81">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 3-PHOSPHATASE AND DUAL-SPECIFICITY PROTEIN PHOSPHATASE PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatase tensin-type" evidence="5">
        <location>
            <begin position="30"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="4">
        <location>
            <begin position="120"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="275"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="428"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="286"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="467"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="484"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="530"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="545"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="559"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="593"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4364447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="621" mass="68012" checksum="9CD8EB097D67D44D" modified="2023-11-08" version="1">MVRKKPRRRRSQTILIHCQASLLRQIVAGPRARHPEAGLDLCYVTDNIIATSGPSGTYPQRAYRNPLDSLVKFLDSKHGEDWAIWEFRAEGTGYPDSEVYNRVYHYPFPDHHPPPFALIPNIMASMRNWLHEKDGRVVVVHCKAGKGRSGTASCSYLISEEGWPVHKALNRFTERRMRPNMGKGVSIPSQLRWIGYVDRWAKHGKLYVEKQVEILEVHCWGLRDGVKIQIEGFVDDGKLIKNFHTFTKDEREIVRGEVKTTGMAQAVQEVMYKNGFGPSQSKKDVSSAQKDGSKNASSSSLDKPDNLDGAAASRKTSQESLPFGTGDVVFRPAKRVVLPTNDINIDVERRNKAAFDLTMVTAVAHVWFNAYFEGHGPEKNGNADESGIFEIDWEAMDGIKGSSRKGTQAFERIAVVWRALSANEGRPGIVITEPKEGEEVHQPAAADWRGAQGVEPGEGKDLGVRTGSPTDSQADISRASSVRSENKKSDVIAKAPGRDSSDSDTQHVRSHGPNGEEILNAPSPWTNAEASGSGPAASASDPVLPSQTDGTSSTASDSRPQDPESHRDGGHVEKVTGSLKYHVSGAASTSDLPDGKPEHDMKNAKEHGLGHLHMGKKKSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1D1</accession>
    <name>A0A9W8Y1D1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Hsp90 cochaperone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">STI1</name>
        <name evidence="9" type="ORF">N0V83_009407</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">Part of a larger complex that includes HSP70, HSP90, and immunophilins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1D1"/>
    <dbReference type="OrthoDB" id="2423701at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042030">
        <property type="term" value="F:ATPase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.100:FF:000004">
        <property type="entry name" value="Putative stress-induced-phosphoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000010">
        <property type="entry name" value="Stress-induced phosphoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000020">
        <property type="entry name" value="Stress-induced phosphoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.100:FF:000002">
        <property type="entry name" value="Stress-induced-phosphoprotein 1 (Hsp70/Hsp90-organizing)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000027">
        <property type="entry name" value="stress-induced-phosphoprotein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041243">
        <property type="entry name" value="STI1/HOP_DP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006636">
        <property type="entry name" value="STI1_HS-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904:SF523">
        <property type="entry name" value="STRESS-INDUCED-PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904">
        <property type="entry name" value="TPR REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17830">
        <property type="entry name" value="STI1-HOP_DP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00727">
        <property type="entry name" value="STI1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="2"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="70"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="STI1" evidence="8">
        <location>
            <begin position="150"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="335"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="395"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="STI1" evidence="8">
        <location>
            <begin position="532"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="202"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="251"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00064323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4363954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="584" mass="64459" checksum="4C5E6BB3B8BBC431" modified="2023-11-08" version="1">MADALKAEGNKLFAEKKFAESIEKFSQAIELDPSNHVLYSNRSGAYASLKDWQKAFDDATKTTEIKPDWAKGWGRKGTALHGNGDLVGATDAFGEALKLDPNNAQAKSGLDAVKRAIESEAMDDGLGGDPSAGLGGMFSAPDMLQKLAANPKTAPLLGDAEFMSKLQQLKSNPNLVGTYMQDPRFLQVMSVLLGIDMQFGGPGAGGPGGADSKEAEEDVDMPDARPAPAESSKKAPEPEPVPEPEEETEEDKAAKEAKAKADELKKKGTEFYKKRQFDDAIENYNQAWETHKDIAYKTNLGAAKFEKGDYEGCIQACQEAVEYGREILADFKIIAKAFARIGTAYEKLGDLTNAVNYYQKAQTEHRTPEVLAKLRAAEKAKIKQDKESYINPEEAEKARELGNAKFKESDWPGAVEAYSEMIKRAPEDPRGYSNRAACFIKLLEFPSAVQDCDEAIKRDPGFIRAYLRKAQAYFTMREYNKCINVCAEAMAHDKDGKNSREIQQQETKALQAQYSAREGETEQQTMERIQRDPEIVGILQDPVMQAILQQAKDDPAALQEHLKNPGIRSKIQKLVHAGVIRMGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1H8</accession>
    <name>A0A9W8Y1H8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_009489</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxoprolinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5404895at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049517">
        <property type="entry name" value="ACX-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF23">
        <property type="entry name" value="HYPOTHETICAL 5-OXOPROLINASE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19278">
        <property type="entry name" value="Hydant_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="4">
        <location>
            <begin position="6"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="2">
        <location>
            <begin position="210"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="Acetophenone carboxylase-like C-terminal" evidence="5">
        <location>
            <begin position="509"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase B/oxoprolinase" evidence="3">
        <location>
            <begin position="717"/>
            <end position="1237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4364034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1359" mass="149405" checksum="4604DFDD15E0CE9C" modified="2023-11-08" version="1">MAPGYRLGVDVGGTFTDVCVITPNGEAVRTKVPTNTADQSIGVQDGVVQARKILKEKYDWGGKFEYIHHGTTTGTNAVLEGKGAKCGLIVTKGHKDILTVRRSQIPGGLGAWINYIQPEPLVPLERTVEVKERIKIDGTVWYELDEKGFRQDLQDLKRQQPQAIAVSLLNSFSNRIHEEKIREILNDEFGPDVEVVTSTDVLPEIQEYERTVTTTANAIVKPVVKKYMQNLQKMLANDTDTIRILKSDGDLTSVDLAGETPVHILMSGPAGGVKAVSHLVAKNTPFKNLITFDMGGTSTDCALLSGTDAIIRRETEVGHLSVRAPSVDVRTVGAGGGSIAIYNEFTKNLRVGPESSGATPGPAAYGKGGTQATVTDANLTLGYLPSKLLGGKFELDVAASAAAVESMAKQMGSDTTTAAEGIIDLVNESMHGALRLVSVEQGYDPRDFALVAFGGAGPLHANSLGKLLGSWPVIVPLSPGVLCAQGDATTKMSHEQSCTYIKVFSDITANDLTKTLSGLKDSCVKTMKNSLANEDAALKVVYQSDMRYKGQAMTLTVDFSEDELNDTQTLLDTLRTKFNEAHEQQFSFSHAKAEIETMRMRAKVIDASEEVAIKPVEKAESSTPPEDAIDFKQNIIYEGKKVEATFWDRTKITKAGIKISGPAVVTEMDANTLILPGHFGEIDSMGNILIWPEEKKAETKVAHTKESAIELVKEQPLIATLIASALGSIRREMDTLMLRCAMSPAIREQQDEFNVITNTRGQMLVGQFGSFITQFLNGWKGTVEEGDIFVTNDVYQIDGAVSHLNDVIILLPIYFEHKLIGWAANFGHLTDVQGKVPGSMSINASTIYEDGLQIPIVKLYEKGVYNEGLASVFFRNSRMPEWFQSDLVALVTACRMAAARVNELCERYGLELYEAATDYLLEQNKIAIKTIIDEKIGNEKSSFTDFIDDDGQGIGPYAISCSMQKEGDKLVFDWDGTSPQSNTSMNYYLSITMFKMFIGYYLLAVYDPHAVVNDGFHDLIDIKIPTGTILRPVRPAALSCRTHLLGRVMDVMQALFGQRNAAYRCAAGFSDSPHLFYSGFKPDGEFYLLYQIGFGGVPARPIGDGPDCHCLFPAIKSIPAENIELYFPVILEANEALPDSGGAGYFRGGNAQRTLYRWLCEGDVSIHDDRWMIKPWGVNGGMPGSRSRKVLYRNNSYGTEREKDNMELCQSKQDYIHVYPGDVLEWVTWGGGGLGDPLTRPATIVAQEVHRRLVTVSGAAKNYGVVVRSSDFTVNEGATTELREKMREERKKTGYSEEMSIDRGGTMRELMAKCEEETGLKPPRPQWEKDPYGPHVGLPYVKEWYKRMREGGMDVFDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1H9</accession>
    <name>A0A9W8Y1H9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein P1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">60S acidic ribosomal protein P1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ALTA12</name>
        <name evidence="9" type="ORF">N0V83_008865</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the elongation step of protein synthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">P1 and P2 exist as dimers at the large ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1H9"/>
    <dbReference type="OrthoDB" id="2194681at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05831">
        <property type="entry name" value="Ribosomal_P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696:SF10">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="66"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="89"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4365246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="111" mass="11186" checksum="2B04CD0E01090083" modified="2023-11-08" version="1">MSTSELATSYAALILADDGVDITADKLQSIIKAAKIEEVEPIWTTLFAKALEGKDVKDLLLNVGSGGGAAAAPAGGAAGGAAAGGDAAPAEEEKEEEKEESDEDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1U6</accession>
    <name>A0A9W8Y1U6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">T-complex protein 1 subunit zeta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">CCT-zeta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CCT6</name>
        <name evidence="10" type="ORF">N0V83_008359</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1U6"/>
    <dbReference type="OrthoDB" id="10052040at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03342">
        <property type="entry name" value="TCP1_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000058">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000004">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012722">
        <property type="entry name" value="Chap_CCT_zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02347">
        <property type="entry name" value="chap_CCT_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4365739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="546" mass="59517" checksum="7E40EF50CAB6B7B3" modified="2023-11-08" version="1">MSAAQLLNPKAESRRRGEALRVNISAGEGLQQVLASNLGPRGTLKMLVDGAGGIKLTKDGSVLLKEMQIQNPTAVMIARAATAQDDMTGDGTTSVVLLVGELLKQADRYIAEGLHPRVITDGYDIAKTETLKFLDEFKLEKQVDRELLLSVARTSLSTKLDSSLAEKLTPDIVDAVLAIYQAPAKPDLHMVEIMTMQHRTAADTQLIRGLALDHGARHPDMPKRVENAYILTLNVSLEYEKSEINSGFYYSSAEQREKLVESERRFVDDKLRKIVELKKEVCGNDPKKGFVIINQKGIDPLSLDVLVKNGIFALRRAKRRNMERLQLSCGGVSQNSVDDMTPDVLGWAGLVYEHQLGEEKYTFIPSHKKQEDVKEPKSVTLLIKGPNSHTISQIKDAVRDGLRSVYNMIVDGSVVPGGGAFQVACAAHLEGEQFKKTVKGKAKWGVSAFADALLVIPKTLAANSGHDIQDSLAALQDEHAEGNVVGLNLATGEAMDPTQEGVYDSFRVLRNCIASATGIASNLLLCDEMLKARQMGRQPGPGGDDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2J5</accession>
    <name>A0A9W8Y2J5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_008183</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2J5"/>
    <dbReference type="OrthoDB" id="201656at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08267">
        <property type="entry name" value="MDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000147">
        <property type="entry name" value="Reticulon-4-interacting protein 1 homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050700">
        <property type="entry name" value="YIM1/Zinc_Alcohol_DH_Fams"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11695">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11695:SF647">
        <property type="entry name" value="ENOYL REDUCTASE (ER) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="7">
        <location>
            <begin position="19"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4365563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="362" mass="39437" checksum="7B16166F7D6FAC1B" modified="2023-11-08" version="1">MASSILPESNTALTYTQPSSQPHLTHAPLAPPKPNELIVKIKAAAINPVDIQLWGNPVVGWLAGKEEKGIGRDYSGEVIAIGEELEKSGKWKVGDEVYGLCNRATGEGTFAQYLNVQPGEPIAKKPSSLSHHEAAAVPLVVLTAFACLDWLPSESQSPNSKRRVIVSGASGGVGGWCVQLAKKLYNCHVIGICSGRNAEFVKGLGADEVIDYSKQDVAKTLLDGRPGGKKYDLYIDCVGGVEMFNHWYDLLHSSGAYITIVGDKTSRTSMGGPATYFTYPSQIIRHIRGYLFGPRYANVLLYQKSELLEQVTQLAEKKDVEVVVQDVVKGVLSEDGYREAWEKVKDYMVEGRVRGKIVVDIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2K6</accession>
    <name>A0A9W8Y2K6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="3">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_008198</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2K6"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000499">
        <property type="entry name" value="Carboxylic ester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041016537" description="Carboxylic ester hydrolase" evidence="3">
        <location>
            <begin position="17"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="4">
        <location>
            <begin position="38"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4365578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="551" mass="59740" checksum="E3CEDD5AE0B44B70" modified="2023-11-08" version="1">MLKIFGAAALLGAVYAQNSSLPTVDLGYEIYRAASFNSTGNFYNFSNIRFAQAPVGDLRFAPPEAPVENRSAINNGDIGRICPQAGPLWGQYATLFLESLLLGTTPSNQTFVAPGANSSSPVSPRDPRESEDCLFLDVFVPEDILSKAGKGYGAPVLVWIYGGGYTGGNKNYNPAGLLAASGNASNGDVIYVSMNYRLGALGWQAGPSYQAEGGVSNLGLYDQRFALEWVQNYIHLFGGDKNRVTVFGESAGGGSIMHQITAYGGLKGKAPFQQAVPQSPGWQQISSNVQQEDTYSKFLNLTNTSSLAELRALPSEAVIRANFQQVTYDSAWGTFTYGPVVDGNFVPQQPGQLLAQGRFDQDVRVMVGHNANEGTYFTPPYIHSEEALIAQLRLAYPYAPQQSVDEITQVLYPPVFDGSYGYTDQYSRASLIISESVFTCNTNYLSTALKNETYSYLFAVPPAFHGFDVPYTYYDGGALSPLSVLNRTVAIALQDFIASFAEEGAPEAAGIRQFNMYGPDASVLELNVTGIEEVRDSNANARCAWWQKALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2Q7</accession>
    <name>A0A9W8Y2Q7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial distribution and morphology protein 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Mitochondrial inheritance component MDM12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MDM12_3</name>
        <name evidence="9" type="synonym">MDM12</name>
        <name evidence="12" type="ORF">N0V83_007956</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the ERMES/MDM complex, which serves as a molecular tether to connect the endoplasmic reticulum (ER) and mitochondria. Components of this complex are involved in the control of mitochondrial shape and protein biogenesis, and function in nonvesicular lipid trafficking between the ER and mitochondria. MDM12 is required for the interaction of the ER-resident membrane protein MMM1 and the outer mitochondrial membrane-resident beta-barrel protein MDM10. The MDM12-MMM1 subcomplex functions in the major beta-barrel assembly pathway that is responsible for biogenesis of all mitochondrial outer membrane beta-barrel proteins, and acts in a late step after the SAM complex. The MDM10-MDM12-MMM1 subcomplex further acts in the TOM40-specific pathway after the action of the MDM12-MMM1 complex. Essential for establishing and maintaining the structure of mitochondria and maintenance of mtDNA nucleoids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the ER-mitochondria encounter structure (ERMES) or MDM complex, composed of MMM1, MDM10, MDM12 and MDM34. A MMM1 homodimer associates with one molecule of MDM12 on each side in a pairwise head-to-tail manner, and the SMP-LTD domains of MMM1 and MDM12 generate a continuous hydrophobic tunnel for phospholipid trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Mitochondrion outer membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="9">The ERMES/MDM complex localizes to a few discrete foci (around 10 per single cell), that represent mitochondria-endoplasmic reticulum junctions. These foci are often found next to mtDNA nucleoids.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the MDM12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2Q7"/>
    <dbReference type="OrthoDB" id="3356905at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21672">
        <property type="entry name" value="SMP_Mdm12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03104">
        <property type="entry name" value="Mdm12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027532">
        <property type="entry name" value="Mdm12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26544">
        <property type="entry name" value="Mdm12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 9" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="11">
        <location>
            <begin position="1"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="68"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="225"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="225"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4366320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="435" mass="47876" checksum="DF1AA6234CFEC852" modified="2023-11-08" version="1">MSIDINWDTLTGGADGTARTETIRAFIHDKFQQVTLPRFIRSVHVHSFDFGSVPPEIEIKDICDPLPDFYEEDEDYPDDDEDEDEVDGSGFDDGQHIPGNASRPVKERRVKETERGASLAEDHLSSQPHFAKAQSSTKPPANRNFPAPAEHLLSPLLPRASTPGIPGGTSNINYFHLPLGAGLSGATTPLAAVAGAQLQGWLDNSYGRPSTPTNMRLRHAASMNSLTLTPQSNPDPSTRPSSRHQHDDRIESLVESDDRSSQHSYDRTPSASPHRMREKSPEDIQVVAHVQYTGDIKMSLTAEILLDYPMPSFVGIPLKLNITGLTFDGVAILAYIKRRAHFCFLSPDDADALVGGETGFSGLQTDSQGQNPRPIQRPKIGGLLENIKVESEIGGQGNGKQVLKNVGKVESFVLEQVRRIFEDEFVYPSFWTFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3H8</accession>
    <name>A0A9W8Y3H8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ferrioxamine B transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SIT1_2</name>
        <name evidence="10" type="ORF">N0V83_007574</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3H8"/>
    <dbReference type="OrthoDB" id="2241241at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015343">
        <property type="term" value="F:siderophore-iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF92">
        <property type="entry name" value="GLUTATHIONE EXCHANGER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="231"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="269"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="306"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="372"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="511"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="537"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4367044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="567" mass="61603" checksum="96597CD4CEA7754A" modified="2023-11-08" version="1">MSDLDEKATPSPDHSDHETGKTPLIGAQSPGVIRIEAIAEHMTLVNRIFIFFGVFLIAYAYGLDGTVRYAYQPEATSSMGGHSLLATIGVVRAVIAAVAQVCTIIEASCRNISGFAAGAVFYQIGYTCVILLVEVIIADTTSLRSRVFFSYVPAAPFIINTWVSGDVSAAVLANTSWRWGIGMWCIIYPICALPLIISLWWVGQKAKRSGALANYKTPYQRYGGLELAKALFWQLDVIGICLMIIVFGFILTPLTIAGGVSTQWQQGKIIAPLVIGILFIPVWIWWETKAPHPMIPFHLLKDRAVWGALGIAITLNFAWTCQGDFLYSVLVVAFNESVKSATRIASLYSFASVITGILLGLVIFKVRRLKPFIFFGTCLFMVAFGLLIHYRGGAGSSAHSGIVGAQVLLGIAGGCFPYPAQASIQAATKHEHVAVITGLYLASYNIGSALGNTVSGAIWSQVIPRELEKRFGNATEATAWYASPITLIAEYPPGTPQRDAAIEAYKHVQRLLCITGICLCVLLIFFASVLRDPKLPDTQSLPDAENLNGPPEVSKSEKKRSLAFWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3M0</accession>
    <name>A0A9W8Y3M0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007293</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3M0"/>
    <dbReference type="OrthoDB" id="3800953at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="451" mass="51523" checksum="E900C7F44972658D" modified="2023-11-08" version="1">MGDSARDQDNSSRMVDSTSTAPESTATGGNTTPKTIIHDQRRLTEGLESLRIQFKLTDEQLAALPELSEISLLEQNDSHRILSHEGVRFAHPFKLYVGAMKWEMDGEKGRDSAYVYLFEGQRKHMKMLIGSGEANGHWEPMDIMYYATLTKLAYPWNEWEGVSRFVAVVKFYFTLGRIAGLYTVDPGFQFAKTWARDLQGACDELKEENRRSEIARQIAAGEWSTEGSRIRHQESRQEPRHSPILPVHQRTEEAYGDRGIRAMSPPTYVNQPPMIARASLSPPDIFSDLGNDYPTHHFELRRGESLDVPRNRLHSSRSQSSLREKFPTEDDDEVELGASSYRPRSYQGGKAPSAERSRLNPDSQAVVTQNKHGISTRTDHRAVPQTEVTRPIRLDVLRAEHLALIESEYIDLYNSRNETQSRMNEIRGELSDEQYRVLMARIKRRPNVAYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3X3</accession>
    <name>A0A9W8Y3X3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="10">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">faeB-1</name>
        <name evidence="11" type="ORF">N0V83_008078</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>feruloyl-polysaccharide + H2O = ferulate + polysaccharide.</text>
            <dbReference type="EC" id="3.1.1.73"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the tannase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="10"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3X3"/>
    <dbReference type="OrthoDB" id="3039123at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030600">
        <property type="term" value="F:feruloyl esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011118">
        <property type="entry name" value="Tannase/feruloyl_esterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33938">
        <property type="entry name" value="FERULOYL ESTERASE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33938:SF15">
        <property type="entry name" value="FERULOYL ESTERASE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07519">
        <property type="entry name" value="Tannase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0858">Xylan degradation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4366442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="374" mass="40798" checksum="3BFDCF96EA1BE77F" modified="2023-11-08" version="1">MRNLHTGVVVGKQISQAFYGKPHDKSYYLGCSTGGRQGFKSAQDFPEDFDGIVAGAPAFAFNNLTSWSGHFYRLTGPPGSATFLSQALWVQVHIDVLKQCDTLDGYADGVIEDPLQCNYDPSGLICPNGTTNSTTCLTPVQANTVKAIFQPLLNDHGDLVYPRQEPGAEVILAVVLANGIPFPYTADWFRYAIYNDPTWDPTKINGTDYDNAARINPFNIQTWEGDLSAVQKRGAKILHWHGGADYIISTENSPRYYKHVESTMGLSPAQLDAFYRFFIVSGTGHCGGGDGAHAVGQSAAETNSLEPGQNVLMAMVDWVEKGSAPETLIGTKWVNDTKALGVEFQRAHCKFPKKNMYKGVGDPDVVGSWECVDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y411</accession>
    <name>A0A9W8Y411_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Multidrug-resistance type transporter aminotriazole resistance</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ATR1</name>
        <name evidence="7" type="ORF">N0V83_007523</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y411"/>
    <dbReference type="OrthoDB" id="2428527at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17476">
        <property type="entry name" value="MFS_Amf1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF1">
        <property type="entry name" value="LOW AFFINITY AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="277"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="314"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="416"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="479"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="546"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="576"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="653"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="181"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4366993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="682" mass="74207" checksum="EEAD1C6D179F34C8" modified="2023-11-08" version="1">MAADARTHTVSKDDVDRFQQGEWEAPVVERRRGRDDVLPLWRGGPIWAGGHSWAVKKLLDWVRKKKYATSDTPPPAIFAAARFLAGEIIIHTSASAPGQERNTLCTVMVNHGSAIELKDETPSSRSSMFYPNNTSLEDDTHPQILDSHPTNPSMSPSTPHERLDLARTKSIAETMSPIREFLFIGLLCSAQFVTQAGLLNTLNILHTIGSDLGITEPGVLSWLIAGYSLTVGTFILLSGRCGDLFGYKNMLVLGFVWFAIWNVVAGCSVYVDGNGGQVLFIFARVLSGIGPAILLPNALGLLGATYNEGRKKDMIFALFGACAPGGAVVGGTFAGLWSLLWWPWTFWTFGITLIILAVLSTFILPSVPLSSHVRALSPKERIHELDLLGASVGITAMILFNFSWNQAAGAGWNEPYIYTLLIVGLLLIPAFFWIELRVAQKPLIPFHALSTDVIFVLVCEMCGWAAFGILLSSQSSSNISFATIPFLFRNCKLTAPGIFIYYYIQILQQLRHLSPLLTMAQLSPVAISGFIAAITTGHVISRIGPGWVMLISMLAFLTGSTIVATMPISQSYWSQAFVATLIIPWGMDMSFPAGTLIMSNAVERKHQGMAASLVNTVVNYSISIGVGIAGTVETHVNKGGRTVEDELRGYRSAMYLGIGLSACGVLASVLFILKMRMRLKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y472</accession>
    <name>A0A9W8Y472_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007977</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y472"/>
    <dbReference type="OrthoDB" id="3928002at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38049:SF1">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38049">
        <property type="entry name" value="RICIN B LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="228" mass="25174" checksum="A8AE2448D9B14B26" modified="2023-11-08" version="1">MYALTLAGIPISIGANEAVHQQRLLDEEAEADERQEEFYLDVYCDAKSRKKDEVNGAIVVLKDGKVRLWPKDPNTGLPKDDPDDDEAETPHPFTGFYLPFPSDDLPTDPCPQPPSWASSAPYRPHSTRVKTDSKAPSKPSSPKPKLNWLYVDKRTREVKYGPRTEAKKHVVGHWDWTEDDEQGITLEGEESLVAVEEESGVWVGCVLGSGGRCVEGGGDWEGEAGVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4A2</accession>
    <name>A0A9W8Y4A2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">agmatinase</fullName>
            <ecNumber evidence="5">3.5.3.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_006996</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>agmatine + H2O = urea + putrescine</text>
            <dbReference type="Rhea" id="RHEA:13929"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="3.5.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.11" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4A2"/>
    <dbReference type="OrthoDB" id="288726at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019627">
        <property type="term" value="P:urea metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11592">
        <property type="entry name" value="Agmatinase_PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.10:FF:000006">
        <property type="entry name" value="Agmatinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF26">
        <property type="entry name" value="GUANIDINO ACID HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040865512" description="agmatinase" evidence="8">
        <location>
            <begin position="18"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00066392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR036979-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4367414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="403" mass="43906" checksum="5B70CF2D2F47354A" modified="2023-11-08" version="1">MLWSWGAIFAITACVTAHSTHHVENQEPLSEERLAELERKWGTDWGFSGISTFAHLPHTRCLTHPQTTYDIAILGAPFDTAVSYRPGARFGPRAIRAGSSRQTSFRGFNTRANLNPYTSWATIVDCGDIPITPFDNALALRQMSEAFLELGKRSPTEKSAESGTGYLKKPKLLTLGGDHSIALPALRALKEVYGQPIAVVHFDAHLDTWHPAKYPSAWIDPEDMSTQSFFNHGSMFWIAATEGLIANSSSVHAGLRTRLSGDEPQDYEDDAQQGWIRIATDDIDEIGTKGVIETIMNRVGTEMPVYLSIDIDVIDPGLAPGTGTPEPGGWSTRELIRILRGIEGMNVIGADIVEVSPAYDGSAETTGLAAAQVAYEVLTSIVRKGLIEQAKIGGTERSVKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4E0</accession>
    <name>A0A9W8Y4E0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4E0"/>
    <dbReference type="OrthoDB" id="5419608at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040843840" evidence="1">
        <location>
            <begin position="19"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="186" mass="17686" checksum="DF9F00CE3471CD70" modified="2023-11-08" version="1">MKFRTVAALVALAVGASAQTTVSPEEQSSVLMVLATALPAESVSYALASSSAFAAEMASSLSAGNTPSWYQALPSDVKSLLPVLYPGSAEATPTPSGSGSVTLAPSASSSGATPAITSIIDSSSVAPTAANSSTVSVKSATLSASGSSSASASVAVSTGGAVAYPPPASIGAGIGAAIGFLGMLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4F3</accession>
    <name>A0A9W8Y4F3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_008349</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4F3"/>
    <dbReference type="OrthoDB" id="2962993at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17327">
        <property type="entry name" value="MFS_FEN2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000034">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000068">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF91">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="367"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="435"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="47"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4365729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="494" mass="54809" checksum="62720E9F8DB7B6AF" modified="2023-11-08" version="1">MSDVEKQDEVARPNVTKSVDSDEVDRQLGEIDPVYEAKLVRKLDLFIIPIVMLLYLLSFLDRVNIGNARLYGLEEDLNLSASQFQTAVSILFVTYILSEVPSNLVLKKLRPSRWIAFITVAWGIIASLTGIVQSYGGLIACRLLLGAVEGGLFPGMAVYLTFFYTKRELALRIGYLFVSAALAGACGGLLAYGIGHMDGVSGQSGWRWIMIIEGIPTFVLGIATWWILPDSPETAYFLNDQEKAFAAARLKRQTGYTKRAAEFHWDDVKKCFEDWKVWTFCFAQFGSDTMLYGFSTFLPTIIKSIMPKASNAMVQVLTIPCYALGAITYLVVARLSDRQQKRGFYSILLGVVSIIGYAMLISPSSAATHYAGCFLVAMGLYVCVGLPLAWLPTNLPRYGKRTSATGLQLSIGNCAGIMSAFIYPSAEAPRFIKGHAITMAMVAFAITCYAVMWAYLHMLNTRREKGEEEHLIEGMSHEEIAELGDDSPRFRYTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4J3</accession>
    <name>A0A9W8Y4J3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mitochondrial ATPase complex subunit atp10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ATP10</name>
        <name evidence="2" type="ORF">N0V83_007222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4J3"/>
    <dbReference type="OrthoDB" id="17089at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007849">
        <property type="entry name" value="ATP10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28106">
        <property type="entry name" value="MITOCHONDRIAL ATPASE COMPLEX SUBUNIT ATP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28106:SF1">
        <property type="entry name" value="MITOCHONDRIAL ATPASE COMPLEX SUBUNIT ATP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05176">
        <property type="entry name" value="ATP-synt_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="262" mass="29867" checksum="3B2154E2D883A5C7" modified="2023-11-08" version="1">MKPDPNEPQTDPKDEDVFIPKPLGRPIGFKLPPQAGENTGNIKTKKDYSGMTMSQRNLEKRKDLVEKWGTNYFRDFKNIRKYRSGKTFMANPRIFKKEAALYFPNFHGDTLAEKSADTTPVLQGKVTVVNVFSSDWGLNQAQTFTGKSVNAELHNIIAESPGIAQMVDINIEENSMKAWIIALFQWQLRAQRPKNEWAKYFVVRKGVSERIRETIGLLNGRVGYVYLVDEECKIRWAASGNAEGTEMEDLNKGFRRLVNEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4N6</accession>
    <name>A0A9W8Y4N6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ARF-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">GGA2</name>
        <name evidence="13" type="ORF">N0V83_008470</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May play a role in the regulation of membrane traffic through the trans-Golgi network.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Binds to ARF1 and ARF2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the ESCRT-0 complex composed of HSE1 and VPS27.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4N6"/>
    <dbReference type="OrthoDB" id="2018246at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14235">
        <property type="entry name" value="GAT_GGA_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16998">
        <property type="entry name" value="VHS_GGA_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.160:FF:000003">
        <property type="entry name" value="VHS domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000008">
        <property type="entry name" value="VHS domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000024">
        <property type="entry name" value="VHS domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052653">
        <property type="entry name" value="ARF-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008153">
        <property type="entry name" value="GAE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47180">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-BINDING PROTEIN GGA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47180:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-BINDING PROTEIN GGA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50180">
        <property type="entry name" value="GAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VHS" evidence="10">
        <location>
            <begin position="32"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="GAT" evidence="12">
        <location>
            <begin position="195"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="GAE" evidence="11">
        <location>
            <begin position="534"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="335"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="408"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="365"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="408"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="474"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4365849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="652" mass="70593" checksum="91AAA338DE94FFF7" modified="2023-11-08" version="1">MEAASARMAARDRYGAFGEAPTSPLQRFILQACDPQNFEPNLALNLEITDLVNSKKGSAPREAAVTIVHYVNHRNQNVALLALNLLDICVKNCGYPFHLQISTKEFLNELVRRFPERPPVHSSRVQNKILELIEEWRQTICQTSRYKDDLGFIRDMHRLLSYKGYIFPEIRKEDAAVLNPSDNLRSAEEMEEEEREAQSAKLQELIRRGGPQDLQEANKLMKVMAGFDTRNKTDWRAKAAEEVGRIQQKARLLEEMLQGYKPGDQIKEGDVFEELANALASAQPKIQKMCEEDSEDHEAVAKLFEINDSIHRTIERYKLIKKGDVEGANKIPVGTLGRSGAGVSQGPNNTLNLIDFGDPEPAPPAAANSSGASQQPAPKGNALEDDLLGLSLSGDTYGQSGSISLGGSNGSVLGMSGMPVPRSQAQQRTSAQQINDLFASGPGPAPPQISSPSPATFSQPSVQPARSPDPFAALTSTPRQSSPFQYQQSVKPPPAAPATVDLLGGAISAPTSNLAQSSSAANDDDEWNFASSVPDTSKEITVLNSSINVLFNISRETDTALLIQSRISNNTPLPISGLTLQVAASKGAQLQLEPQSGVTLAPSQKHGITQAIRVNNVQQGTGNSVKMRWKASYSLGGQQKNEMGEIPSLGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y501</accession>
    <name>A0A9W8Y501_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y501"/>
    <dbReference type="OrthoDB" id="4204700at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="257"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="414" mass="46619" checksum="9813226C64C148F7" modified="2023-11-08" version="1">MVLEDVDKSARDSFTDNPYPSENDSHASPSSSSGVRFDPSSLPQPLPIIGTFMGFSDGAVRFKTDLTIQYAERKLGRQLDPDESQALAYHMYKLEQTKSYYAAAGATAGTWRWYSTMAKMRYPFYQPKLENIDPNKFAFIRGPMAQFARHTWRFSLYALVAGQVGNVIGQFIAQPIAAMNVSKDPKLEQFAIELKAAGEADNQRNAQQGREIENRRREFQEQVRNREGAGPSPQARWGRQAPKQSEDAGDDMSPTAGNESWESKSGSSESWEGFSNNTSAPPQQRQQSRSLPPRQSTPSSSLPFDDDDASPTGGLFSDEVSKTQAQARPGESTWDRLRRGGAPAPLQRPQQPSHRAEPERKEQKDGSILGDSYTFVEDDEERKRERERAQQEFDARLERERQGKDFSSDEGKRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5U3</accession>
    <name>A0A9W8Y5U3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH La-type RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_006664</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5U3"/>
    <dbReference type="OrthoDB" id="435402at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033167">
        <property type="term" value="C:ARC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08029">
        <property type="entry name" value="LA_like_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018606">
        <property type="entry name" value="Arb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF140">
        <property type="entry name" value="ACHILLES, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09692">
        <property type="entry name" value="Arb1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="4">
        <location>
            <begin position="25"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="567"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="144"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="577"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4368308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="596" mass="67353" checksum="DC395E2A63FF97E7" modified="2023-11-08" version="1">MATATAELPVDAQHLNGVTKSTNDADTDHPDGAEIRRQVEFYFSDENLPTDLHLLQCCGGRENLPVSVSRICGFKKMRGYKPKSLVVAALRKSPFLEVSEDGKTIKRKIALQGKCVLDPDFFDDDEIAYDPRNPKPAVYPVPNIPQTKTQYPPGTSKNMMKPTGFEKTHIEPPQTPQEAVEEAALYDQDKPFVERIEIAIQRFKQKRRMHQMYALVFNKLMRFGGVEWGDRMYQGTSKQEMANMDAEELARALATHNVPWDRSDEKQWIVDFVGVSKAFLSSWYPAHYGYALDAVKNACQVLRSFYNYLLYHKVCPEYNDQLELARKACDLAEQELPMVHAAGLALPGDFNKSGSAIFGGAHAGMYTGDRSWAEEARAEGIRTDEIGIRDEVAKITFMTGVATLGSDEQYEKAAANSFKVLNSEYVSLEIIAIHPADDLTKESYVKQSEMLKSKLEQLEPLGKLVCKRSYVEDCDEWDLPTDKLKFPNGKPEKAGHDIEYVFWVEESVLSKCFEGMKMDAKVLALEGGLTILDDITEVMCSFFTWIPNELWMERKPKEVRWLKKAMAVDEEEEVDDNAVKESGEGKGASDDEFDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y614</accession>
    <name>A0A9W8Y614_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Catabolic 3-dehydroquinase</fullName>
            <shortName evidence="3">cDHQase</shortName>
            <ecNumber evidence="3">4.2.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">3-dehydroquinate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">qutE</name>
        <name evidence="7" type="ORF">N0V83_006362</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Is involved in the catabolism of quinate. Allows the utilization of quinate as carbon source via the beta-ketoadipate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Aromatic compound metabolism; 3,4-dihydroxybenzoate biosynthesis; 3,4-dihydroxybenzoate from 3-dehydroquinate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homododecamer. Adopts a ring-like structure, composed of an arrangement of two hexameric rings stacked on top of one another.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the type-II 3-dehydroquinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.10" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y614"/>
    <dbReference type="OrthoDB" id="8191625at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046279">
        <property type="term" value="P:3,4-dihydroxybenzoate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019631">
        <property type="term" value="P:quinate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00466">
        <property type="entry name" value="DHQase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.9100">
        <property type="entry name" value="Dehydroquinase, class II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00169">
        <property type="entry name" value="AroQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001874">
        <property type="entry name" value="DHquinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018509">
        <property type="entry name" value="DHquinase_II_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036441">
        <property type="entry name" value="DHquinase_II_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01088">
        <property type="entry name" value="aroQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003804">
        <property type="entry name" value="PRK05395.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003805">
        <property type="entry name" value="PRK05395.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003806">
        <property type="entry name" value="PRK05395.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003807">
        <property type="entry name" value="PRK05395.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21272">
        <property type="entry name" value="CATABOLIC 3-DEHYDROQUINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21272:SF3">
        <property type="entry name" value="CATABOLIC 3-DEHYDROQUINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01220">
        <property type="entry name" value="DHquinase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001399">
        <property type="entry name" value="DHquinase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52304">
        <property type="entry name" value="Type II 3-dehydroquinate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01029">
        <property type="entry name" value="DEHYDROQUINASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 3" id="KW-0672">Quinate metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="3 4">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3 4">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <begin position="102"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="3 6">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4368007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="152" mass="16450" checksum="823DBF03D0927019" modified="2023-11-08" version="1">MAKSILLINGPNLNLLGTREPTIYGSTTLPEVIASASDQCARLSITFSHIQSNHEGVLVDRIHEARGNVDAIVINPGALTHTSVALRDALVGVEIPFVEVHISNIHKREAFRHHSYLSDKAEAVICGLGVYGYEAAVEFAARNIKGKEKAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y638</accession>
    <name>A0A9W8Y638_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_008126</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y638"/>
    <dbReference type="OrthoDB" id="269227at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF147">
        <property type="entry name" value="CHOLINE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="5">
        <location>
            <begin position="250"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4366490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="322" mass="34455" checksum="5F0D2AA769B4BC14" modified="2023-11-08" version="1">MSTAGCVIARRLAEDSNTSVLVLEAGRPKEEVPASAIPAGVSQILGTDADWNIQSEPCVELNNRQLHLGRGKFVGGSSGCNGTLCIRGTPQDYDDWAVEGWSGADMFAYMRKAEHFHNKDWFEATNNEHGHGGPLATAPHDLAPISSRVLDSFQSKGLPLRPDMFTTGDAAQGCGHAVRTIIKGVRTTSYDYIGSDHSYPNIKVMTGQHVDKIVLEARGSDLVAAGVEVRQADGQKSYLKAEKEVILASGAYGSPAVLLRSGIGPKSELEELGIPVQVDLPGVGKNLMDHLVSFTSKPIAEVIANSLICRSYSTSTKCHRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6I8</accession>
    <name>A0A9W8Y6I8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ATP citrate synthase</fullName>
            <ecNumber evidence="4">2.3.3.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">acl2</name>
        <name evidence="16" type="ORF">N0V83_006863</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>oxaloacetate + acetyl-CoA + ADP + phosphate = citrate + ATP + CoA</text>
            <dbReference type="Rhea" id="RHEA:21160"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.3.3.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.8" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6I8"/>
    <dbReference type="OrthoDB" id="5295093at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042709">
        <property type="term" value="C:succinate-CoA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003878">
        <property type="term" value="F:ATP citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000004">
        <property type="entry name" value="ATP-citrate synthase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.110:FF:000003">
        <property type="entry name" value="ATP-citrate synthase subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032263">
        <property type="entry name" value="Citrate-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056749">
        <property type="entry name" value="Citrate_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815:SF10">
        <property type="entry name" value="SUCCINATE--COA LIGASE [GDP-FORMING] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="SUCCINYL-COA SYNTHETASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16114">
        <property type="entry name" value="Citrate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24948">
        <property type="entry name" value="Citrate_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-citrate synthase ATP-grasp" evidence="15">
        <location>
            <begin position="2"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-citrate synthase citrate-binding" evidence="14">
        <location>
            <begin position="297"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF24948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4367282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="486" mass="52715" checksum="B910A4589202340F" modified="2023-11-08" version="1">MSAKSIFEGDGKAILNYHLTRAPVIKPTPLPASATHNPPSKLASLHFDADADIKGVLDQAEATYPWLLASGAKFVAKPDQLIKRRGKSGLLALNKTWAEAREWIEARAGKPQRVETVDGVLRHFLVEPFVPHPQDTEYYININSVREGDWILFTHEGGVDVGDVDEKAEKLLIPVDLSEYPSNEEIAATLLKKVPKGVHNVLVDFISRLYAVYVDCQFTYLEINPLVVIPNAEGTSAEVHFLDLAAKLDQTAEFECGAKWAIARSAAALGMPLAPAKDAKTTVDVGPPMEFPAPFGREMSKEEAYIAEMDAKTGASLKLTILNATGRVWTLVAGGGASVVYADAIASAGFANELANYGEYSGAPSETQTFHYARTVLDLMLRAPQHPEGKVLFIGGGIANFTNVASTFKGVIRALREVAPVLVEHKVQIWIRRAGPNYQEGLKNIKNVGQELGLDMHVYGPEMHVSGIVPLALVPGKTTDIKEFSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6K8</accession>
    <name>A0A9W8Y6K8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ethanolamine kinase</fullName>
            <ecNumber evidence="3">2.7.1.82</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_006893</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the choline/ethanolamine kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.82" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6K8"/>
    <dbReference type="OrthoDB" id="10267235at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004305">
        <property type="term" value="F:ethanolamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05157">
        <property type="entry name" value="ETNK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603">
        <property type="entry name" value="CHOLINE/ETHANOALAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603:SF66">
        <property type="entry name" value="ETHANOLAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01633">
        <property type="entry name" value="Choline_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4367312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="328" mass="36735" checksum="7E2BD807C84C994F" modified="2023-11-08" version="1">MKETRSHSLLARHGLAPSLYARFENGLLYKYIQGSVCAPADLRRPEVWRGVAQRLGEWHATLPISSISSTCPAPSQILSQNNNKRASLAAMAQLTPGKPIPNVWTTMQKWISALPIATTAQSTRRDELMHELESLTKMLGDTPGVGGSNSFVFAHCDLLSGNVIIEPSPSSAAASRRSSASDVSEEPETAACVSFIDYEYATPAPASFDIANHFAEWGGFECDYSAMPTRSVRRAFLREYLHSFSAHQNRTYKESELDELFEQVDRFRGVPGFYWGIWALIQAQISLIDFDYASYAEIRLGEYWAWKEAVNGKVEKPTEREVRWAQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y727</accession>
    <name>A0A9W8Y727_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromatin modification-related protein EAF3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Chromatin modification-related protein eaf3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">EAF3</name>
        <name evidence="15" type="ORF">N0V83_005943</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in deacetylation of histones, chromatin assembly and chromosome segregation. May act as a transcriptional oscillator, directing histone deacetylases to specific chromosomal domains. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MRG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y727"/>
    <dbReference type="OrthoDB" id="124855at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032221">
        <property type="term" value="C:Rpd3S complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.30:FF:000004">
        <property type="entry name" value="Putative Chromatin modification-related protein eaf3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.30">
        <property type="entry name" value="MRG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008676">
        <property type="entry name" value="MRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038217">
        <property type="entry name" value="MRG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026541">
        <property type="entry name" value="MRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053820">
        <property type="entry name" value="MSL3_chromo-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10880">
        <property type="entry name" value="MORTALITY FACTOR 4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10880:SF15">
        <property type="entry name" value="MSL COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05712">
        <property type="entry name" value="MRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22732">
        <property type="entry name" value="MSL3_chromo-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038133">
        <property type="entry name" value="HAT_Nua4_EAF3/MRG15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51640">
        <property type="entry name" value="MRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="14">
        <location>
            <begin position="25"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="88"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4368861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="324" mass="37169" checksum="C1558D0A7A698CC7" modified="2023-11-08" version="1">MAPAAAPEPSYRKDEKVYCFHHELLYEAKVLEVKAVDGDDKKSGFQYRVHYKGWKNTWDDWVPEDRLRKLSPENRELANNLRHEMLAQQRAARAQPPPSKKKAQGSARGSEERQTSVSAAAPRGQKRMRDNDLEKEEVFQNKLAVRIYMPDRLKSLLVDDWENITKNLQLVQLPSSKPAGVILDEYSDHVSASGNRNSTEKDITEEVVAGLKEYFNKALGRLLLYRFEREQFYDINTRIDHPTDELAGKTLADIYGGEHLLRLLVSMPELIAQTNMDHQAINRLREELSGMTTWLSKEPQVNTYFASVYESPGQAYIDKVKSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y790</accession>
    <name>A0A9W8Y790_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carrier domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_007242</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the NRP synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="408177at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05930">
        <property type="entry name" value="A_NRPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05235">
        <property type="entry name" value="SDR_e1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000047">
        <property type="entry name" value="NADP-dependent L-serine/L-allo-threonine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010080">
        <property type="entry name" value="Thioester_reductase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01746">
        <property type="entry name" value="Thioester-redct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44845:SF6">
        <property type="entry name" value="BETA-ALANINE-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44845">
        <property type="entry name" value="CARRIER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="6">
        <location>
            <begin position="535"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="513"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4367657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1282" mass="139549" checksum="255C26C4A2D09558" modified="2023-11-08" version="1">MSVIDTTKDLSVLFTKQVQATPDLIAVEDEKYTYTYQQLHDKVVALADRLRGHGVGRDSLVGVLLPRSADYVIACLAALRAGGAFLVLELAYPPDLLADVLEDSQPSVVVTVSAEVGKIREGTPLVVLDEQNSTSNGHVEDSELPPLPAETDLDRLSFVAYSSGTTGKPKGIANPHRAAVLSYNLRFGLSDLQPGDRVACNVFFVWEIIRPLLRGATVVAVPDDASYDPRMLVDLLASKKITETLFTPTLFSAVLARHQALSTRLPDLKTVWLNGEVVTTDLARRGIKALPNTRLLNVYSACETHEIACGDIKDMLEKLDKDAPYCPVGPPLIDSKYIYILDESGQKVDEGENGELFVGGHLLARGYLNRPETTAKAFIRNPFSSKTGARMYRTGDKARLLPNGLLEITGRVGAMIKIRGYSVVPGKVESAIVNHLAASHCAVVPFGEGIDRQLVAYFVRDKESSAERPVVEIDRTGRSSTARRMLMPYLAHYMIPSLWVELDKLPTSDVSGKVNLKALPPPRPSSPTGSARKFEQSPITVEQITESWASILGVNADTITPEYNFFDLGGHSLLLADLAARLSNTFGFRVPVARLANPATLNGHLDTVRAIRDGHTAEVQENLPAVLRADSVLDKDIQPQNPKVCALKDAKTVLLTGVTGFLGAFLLRDLLETTSARILCLVRFADSSQDDIPAGIARIRRNMLDFGLWSDNIMERVEILPGNLSRKRFGLPPDTFKSLADRLDVIVHAAATVNLVYPYAALQKPNVGGTREVLRLAAQGGTTVQYISTNGVLLPAAGREGWSEDAMLKVDDVIKLADGYGQTKWVAEQLALEAQRRGIPIKIHRLGTISGHSETGAANAWDLLTALIVESVRIGHYPDVKGWRAEMTPVDFVSKAILHLSNQTETEQTVFHIGDPDPVDISKVFEDLNALGYPTKPIEFEKWVALWDDKRGSAKGGDGAFTVDILRSGMPSIEFLRDVVVLDNAKTRPLRLAVERPKVDQVLLETYTRHWYARGWLPNPPLRQDSAGGSARLPRRGPLFGKVAVVMGASSGIGAATATALAREGCHVALAARRVDALEDLKKRLVIREGKIITQKTDVTDKAQVEALVSAAEKQLGPVDIFISVSGVMYFTMMANTQTDQWNQTVDVNCKGLLNVISSVVPSMLKRNKGHLLAISSDAGRKVFPGLGVYSASKFFVEATLQSLRLETAGTGLRVTSVQPGNVATDLLGMSTDQEALKKYGEPSGAKVLDPENVAECIVYALKQPEHVAVNEVLIEPRDEPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7H9</accession>
    <name>A0A9W8Y7H9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Probable beta-glucosidase E</fullName>
            <ecNumber evidence="5">3.2.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Beta-D-glucoside glucohydrolase E</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Cellobiase E</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Gentiobiase E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">N0V83_006215</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Beta-glucosidases are one of a number of cellulolytic enzymes involved in the degradation of cellulosic biomass. Catalyzes the last step releasing glucose from the inhibitory cellobiose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan metabolism; cellulose degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7H9"/>
    <dbReference type="OrthoDB" id="416222at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.300:FF:000002">
        <property type="entry name" value="Probable beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1700:FF:000003">
        <property type="entry name" value="Probable beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050288">
        <property type="entry name" value="Cellulose_deg_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715">
        <property type="entry name" value="BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715:SF20">
        <property type="entry name" value="BETA-GLUCOSIDASE E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type III-like" evidence="23">
        <location>
            <begin position="908"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="857"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="857"/>
            <end position="873"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00039576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00041599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00041811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ4369131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1011" mass="111249" checksum="9485438F0032D9D3" modified="2023-11-08" version="1">MHWRRAIYGDMGFTMPPYESHELRRGKYARLDAPADDDLDTPMRFSSDSNATLEDLEEHDPLAFDSKLKRKKSAGDLKIRKASYKDTESAVTGPFARRQSKCAPGRWCCWLLTFIAAVVLLLASGGIWAWRATAPHDGLSPPWYPAPKGGTDKTWEESYKKAAELVKQMTLPEKINITTGTGWSMEMCVGNNGAVPRLRFPSLCLQDGPLGIRFADNITAFPAGITVGATWNKELMYLRGKAHGKEARLKGVNVLLGPAMGPLGRMPAGGRNWEGFGSDPVLQGIAAAQTIKGIQDEGIMATAKHYLLNEQEHFRQAWEWGIPNAISSNVDDRTLHEIYAWPFADSIRAGVTSIMCSYNQVNSSYACQNSKLLNGILKDELGFQGFVQSDWLAQRAGVASALAGLDMTMPGDGLRWAKGDSLWGAELTRAILNGSVPVDRVDDMVTRVVASWYQVGQDKWDALGPNFSSWTNDRVGKLHEGSPSDTSMGVVNKFVNVQGQGKDAHGHLVRRVAAEGTVLLKNEGKVLPLNRKGRKTRRKNKRQTKYRVGVFGEDARLPHEGINHCNDQSCNDGTLASGWGSGAVNFTYLVEPMSAIRDAFDNETVYVTDWLENRLPSQKEILKDQDLCIVFANSDAGEGFEQYEGIRGDRNDLYLQKDGDRLIREVAQDCGEGKGDVVVVIHTVGPVIIENFIEITNVKAVIIAHLPGTESGNAIVDLLFGDVNPSGRLPYTIAKKEDDYGPGSKVKYLPSPTDGLSPQQNFSEGLYIDYRYFDKQDITPRYEFGYGLSYTQFKLSSLVITSKGNMNPLPAPRPSGLTPPSYSTDIPDPQSALFPPNWHKVEKYIYPWLDSTDGIKSPTTTTKKAPQVESSPPLSDAGGGSGGNPDLYTTLLTVSATVSNIGALDGATVVQLYISLPQGYKDHETGEAVDFPVRVLRGFEKVYLEAHKAEQGIGGGEGTSKEVTFEVTRRDLSYWDVRRQNWVLPQVKEGEGGFDVRVGFSSRNLPLEGKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7J8</accession>
    <name>A0A9W8Y7J8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_005578</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7J8"/>
    <dbReference type="OrthoDB" id="3598904at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052360">
        <property type="entry name" value="Transcr_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36206">
        <property type="entry name" value="ASPERCRYPTIN BIOSYNTHESIS CLUSTER-SPECIFIC TRANSCRIPTION REGULATOR ATNN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36206:SF12">
        <property type="entry name" value="ASPERCRYPTIN BIOSYNTHESIS CLUSTER-SPECIFIC TRANSCRIPTION REGULATOR ATNN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4369814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="472" mass="53498" checksum="C38FCABED87DED82" modified="2023-11-08" version="1">MYLAPALQFSTAEERESFDFFTSRAVSSLRGSLDSSFWQRELLQAAYREPAIQHCVIALGAMHRRFFQGKGSHINAESLSDNYLQFALRQSNQAIQDLVKQGHRIMTTRADKVTLMTCSILFFSMSCLQGYQKDAFEHLRSGIRMLNEMDSTENSKSEEHPVDVESLRSIFVGLDLQARSIMSTSQTGTWVTKPKTKATFILPPPELNMDSLLTMMRFLESIYTQIHAFFQSTASRPEENADDINLEYRDLMTRFHRGTTVLEAICGKSTSTADEFTEPLTALRLQQSAMEFLLRSPPRPDLETRFSFIRKFASDSDLYDEPFDASAQVVKLFELATRLLPISSTLTPVFTMGIGPLSALWLVAMRAPSTFLALRKRAISLMLSHPRREGFWDGMLAGQIAQEALRLEQESTQAEFGLSANPSHDLITPDDLRSAIVAITYEENDGRRAKIQYFNSRDLKAGRPGSMQWINW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7L1</accession>
    <name>A0A9W8Y7L1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PAN2 UCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005551</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7L1"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028881">
        <property type="entry name" value="PAN2_UCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13423">
        <property type="entry name" value="UCH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAN2 UCH" evidence="2">
        <location>
            <begin position="65"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="544" mass="61436" checksum="46CD176C6B7086A3" modified="2023-11-08" version="1">MPPTTRARARAAGATPLFSLKTDLQRKRDHYGQKLKARWPLQRQRPHRNPRGIHIGPHNSRTRQNVVLQSLMHLPIFLGWLNSHNDHQANAANCTTCALKLLAREYWAPHNANIPFPDTHQALTLIADSATADRQALVDAYDFYEWITAHNQMGDPQNALLNDPRNPWDNEHKALFQLDVKERQQCLTCGLFTGPAAPRQYGIVEPQYDARAPQYQNALRTLDAILADWFTDDQWPENCQTCRSEQPFRYSRTIEAAPQVLRVKVLFNIDIDGHILKQCQPFAIQDPLDLSQHQENQGLPLLYTLSSAIAHGGLGVAEAAEYETGLRVRAGGESDEEEDSSEPLALESDESDIADYLCELRERRGMFQTAPPPPADLPDASDDSYEGEADGDDMFTHAQHDGSPPPFDNNPRIHVAPPTRDDLPSPSPSASEQEDEEDHTIERDFEVPWDNSNGPVLTADIRLENPPDWIVNVRGPADDSHISDDHFNNLQHGERLDSNPQMPGEANDPACWRQDGYQVYILTYTRNKQVGRFGRMERDIPAFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7P6</accession>
    <name>A0A9W8Y7P6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RSC complex subunit Rsc7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_007214</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7P6"/>
    <dbReference type="OrthoDB" id="5598844at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013933">
        <property type="entry name" value="CRC_Rsc7/Swp82"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22597:SF5">
        <property type="entry name" value="LOCALIZATION PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G10600)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22597">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08624">
        <property type="entry name" value="CRC_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="422"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="485"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="520"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="572"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="594"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="624"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="644"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4367629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="668" mass="73493" checksum="AA0703814A03F7EF" modified="2023-11-08" version="1">MKNQLTRPPGVLVGPPQPVAQQPSRGLKRSRSPENSYGDVQHGDDGIADQVDTDEGKPRKRGRPPKTTKVGFAGSPNRGSQPPSTPSHTGPAPPVQTPQLQSTPLPQASPPQASPSTKSTPTKPAVLKALPTVRDHTTDQLNPERDEYIPRETDEAGERKVTLTGHPLDGREYRCRTFFVPNRGDKLFMLATECARVLGYRDSYLLFNKNKSLCKIIATQAEKDDLIHQEILPYSYRSRQIAIVTARSMFRQFGSRLIVNGRRVRDDYWESKARKQGFTEEDAAGEKRPGAAKAREAAAAEANHANAMAFAHPGAYTGNNQDYLGAAINPLQPFGGLNPAPGSMPTGNSDPYNQRTTTDLQHRDYSGVQRPRHEMTGLPYQDITRPTPSGEILTQAAQTAEVNKHLEQNRKHRKEYLNDVWTRPHEPPVQPNRPATAEGEHVPQVTQAVQSPRLPSTNVTMPGNQYGMAAQTPQRPGQPMGAQSYRPQSLPQNNMVPSPMAQQHTPLRPDQMHRRPPSIGMPQNMTPTGMGPTGMLPGVQNSGHGYPPSHMWQGPPQPSPLSQQHNMQQYAQRPPQQSPLPQQSPMHASPQLHHAQSSVSMHGTPVQQYQTMGAMGGDPGYQAMAQNPYQPSPSPHQFMQHQSAAAQQPGMPGWAPPQGQQQQGWQSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7Q3</accession>
    <name>A0A9W8Y7Q3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WLM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005349</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7Q3"/>
    <dbReference type="OrthoDB" id="49605at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013536">
        <property type="entry name" value="WLM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47795:SF1">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE WSS1 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47795">
        <property type="entry name" value="UBIQUITIN AND WLM DOMAIN-CONTAINING METALLOPROTEASE SPCC1442.07C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08325">
        <property type="entry name" value="WLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51397">
        <property type="entry name" value="WLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="15"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="WLM" evidence="2">
        <location>
            <begin position="128"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="35601" checksum="BA3B53D4EF7AADF4" modified="2023-11-08" version="1">MGDSNDENTPAASADELQIKLTHHGKLIALSFAQDATISDLSDRVSADFSIPPSNQKFLIGGKLGLQKPPFKDPTIPLTDLVSKKITLMGSTSAEVSSLNASISAASAPRRPRPIKAATPARNRDYKKIQEEAQYTFHTLRPLPYLPHPERSLRFLERLRDDAGIKAAMRTHKFSVPLLTEMDPAMHTTRESKTLGLNRNQGEVIELRLRTDAYDGYCDYKTIRKTLCHELAHNVWGPHDRNFWELCKQIEREVLRDDWRSGGKSVGNQVYYEPDEGDEVHDHGGWTGGEFTLGSGGGGGEVVGRRWLVVRVGVCRWEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7U7</accession>
    <name>A0A9W8Y7U7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RmlC-like cupin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005923</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7U7"/>
    <dbReference type="OrthoDB" id="2588190at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02215">
        <property type="entry name" value="cupin_QDO_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053146">
        <property type="entry name" value="QDO-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36440">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_8G07350)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36440:SF1">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_8G07350)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="365" mass="39982" checksum="73EFE37A1EBF9CEC" modified="2023-11-08" version="1">MPNSSTDSSTSSHNNVIQRVSNPPGKSTPYIIPAYEGETMTIPGTKSTIRILASAKETEGMMSVFGLDGTVADPPGFHYHNLAHDVFMCTKGRLKIWAGDRCKILYPGDFAYVPPSVVHQPMLLDAGINETVGLVTPGQWVDFFRFVSEEYDGVVANEVDTRNPGAVFGPKIREIKEKYDVVFQPHFQGAEVSEWTSEDSKLPDKQEAYYLKANTGPCHLLEGVLSRPFIMTKQSEGPTGNFAITSIESSNRLSNTILSKPFAFERVHQVYHVLDGAISLTTDGGRGNLVRAGETAFIPAGTKVSITFIDRYVRLWAYSSGDGLETLIAEAGGVFEGSIVPDQIREVDTDLVQKVAERLDVKLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7X7</accession>
    <name>A0A9W8Y7X7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mannitol 2-dehydrogenase</fullName>
            <ecNumber evidence="4">1.1.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_005758</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the NAD(H)-dependent interconversion of D-fructose and D-mannitol in the mannitol metabolic pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>D-mannitol + NAD(+) = D-fructose + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12084"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16899"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.67"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the mannitol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.67" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7X7"/>
    <dbReference type="OrthoDB" id="418169at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050086">
        <property type="term" value="F:mannitol 2-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046029">
        <property type="term" value="F:mannitol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000129">
        <property type="entry name" value="D-mannonate oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000669">
        <property type="entry name" value="Mannitol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050988">
        <property type="entry name" value="Mannitol_DH/Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013118">
        <property type="entry name" value="Mannitol_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013131">
        <property type="entry name" value="Mannitol_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43362:SF1">
        <property type="entry name" value="MANNITOL DEHYDROGENASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43362">
        <property type="entry name" value="MANNITOL DEHYDROGENASE DSF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01232">
        <property type="entry name" value="Mannitol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08125">
        <property type="entry name" value="Mannitol_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00084">
        <property type="entry name" value="MTLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mannitol dehydrogenase N-terminal" evidence="8">
        <location>
            <begin position="123"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Mannitol dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="385"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4369994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="588" mass="65169" checksum="C1BAF92CF257D5E9" modified="2023-11-08" version="1">MAPANPSRQAQSIVALVHKRYASTLRNSAQTIPRTIVANGISTKAPRTTAVIPTTQISQRKNHTASLPTLHTRKFTHAAQNAAQAAAAVPEPEVTSLRLNNHNLSQLHNATVPSYDRTTIKQGIVHVGVGGFHRAHLAVYVDSMLEQLGLRDWSICGVGLQPFDAEMKNALAPQDHLYTVLEKSARGSKARVIGSITDYLFAPDSSEAVIAKMAHPDTHIVSMTITESGYFYNENTHELEIEHPDIAFDLQGKGSPRTFFGYVSAALERRFKAGLKPFTVLSCDNMQKNGDITRNMLLTFVKHRNPEMATWLAQNGAFPNSMVDRITPRTLEADVNDLAENFGVQDSWPVVTEPFMQWVIEDKFADGRPPFEKVGVQVVKNVKEVEEFELTKLRLLNASHSAMGYAGYLAGFQYIHEVIGHPKFHKYIFNMMQQEVKPLLPRIPGVDVDEYCKTLISRFANPAMKDELTRICLGGSGKLPQFIMPSIAEQIVAGGPLRRLTLCAAAWFTYLQGFNEKGEEFVLSFDPMAKELQPLAREGGSDPMKMLGVKSLFGDDLRGDERFVAELKAAMKSLKTEGALATIEKYAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8A2</accession>
    <name>A0A9W8Y8A2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_005666</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-binding monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8A2"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050775">
        <property type="entry name" value="FAD-binding_Monooxygenases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43098:SF4">
        <property type="entry name" value="BLR3857 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43098">
        <property type="entry name" value="L-ORNITHINE N(5)-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="609"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="631"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="646"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4369902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="691" mass="78236" checksum="A56806529E9DFAD4" modified="2023-11-08" version="1">MATEGPVVPASVAEVPITLPHLEKSLVDIKTGEISQEETLPFDPVTLKEKYLAERDKRLARDQGVEQYVLLDDHLSQYLKDPWVEPGFTRDPIEEETDVVILGGGYGAQVVAVRLIEQGVNNFKLIEKAGDFGEVGFMPTEKYARAKELLKHSRIIGEKYDLYSRALFQTEVKDMRWNEEEGKWTTHTARGDKIKSRFVIPAAGPLHRPKFPGLVGIEEFQGHSFHSSRWDFNCTGGDPDGNLDKLKDKRVGIIGTGATAVQIVPHLGEWAKQLYVFQRTPSSIDLRGNRPTDEEWAKSLEKGWQKKRMDNFDSIVNGGFEIEDMVADGWTDIIRELLTAAIDPNNPVEAAAKRQLADFKKMEQIRARTDEIVKNKETADGLKPWYNQFCKRPCFHDEYLQTFNRPNVKLVDTRGQGVDRITQNGVVANGEEYEIDCLIYATGFELANEWAHKTGMEIYGRNGITTTEKWKDGAMTLHGWGSRHFPNCFWVQIVQAALTPNFMHVTNEQAIHYAYIISECLKRNIRTIEPTQEAEEGWTDTIVKGTALRMDFFKECTPGYYNNEGKPSKVGARNATYGYGSPAFIKILTEWRAAGDLAGLDIRFFPVPSAQPVSKDTNTTETTTKSEKSAQVETESQPRVNPNPGTPASRSENKSIVQRYSDLQAKHQTEINDLVWRHKGEIERLLRKHDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8D8</accession>
    <name>A0A9W8Y8D8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005726</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8D8"/>
    <dbReference type="OrthoDB" id="10588342at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="403" mass="45733" checksum="56BEA37F45D1156C" modified="2023-11-08" version="1">MHHKAVHAGTAKHSLAEQLPVLNWVVLGLVSAGAGLHLIRRYRKKNPQIPIPPSQDRKKWRVLARGITAWLQQAMRSNAALRRDLQKAQQAAEDMRRGKQVACKKAAGALQEAQDLRKTLEDKDEDARELRKALEKEQAKVLELRQHIQHAEELERMQDEEITVARECEEQLQQELRMETAHRKQLQQRLVARSRLPDRSLRERLTTHQLDESRPPKTPQQNYGSIYESVERILRRDAARVENTPTNPRKRGRQDGGGEEASRPRKLQRSSSSERGEDVAYTPKGTPPSCSYLEGDVKERVNPRIWGLDGLPAIKVGPTRPLQTPVGADNGMSEDAMGDDIVESIETPVPSYIARRARSRLPVPSSRESPVRRSARAATRGVKSLNEREMALRSMSPEKDKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9X1</accession>
    <name>A0A9W8Y9X1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005352</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9X1"/>
    <dbReference type="OrthoDB" id="3438382at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="863"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="99"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="874"/>
            <end position="886"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="886" mass="98217" checksum="8A1FB1E06AB1CB32" modified="2023-11-08" version="1">MSDTTARNNVQISSAGSNGSGGSTPVQAAPPVPSMLPVSEASRSTGRAAGQKAPMTMESLQSVRTRKQAVGRLAPTGVVAEQPSATNPNSSQLQIQLQLKAQEIEIAHLKKVLEKVSDTCTKPEDLPVQPTQQWMVDRIEKTESANRALLQKTDRLEAMALKREEDKKAVDTILETVPDIKRSIDSLYTWKKTQEAKSMVLRQDGVTLEKAMTTLQGEVANIDSQLTKRIDEQQKTLGSLSSGLNITLEFQKDVKKRDIFHKFDALSEKVTGIEKNESHTYEKTSSIGRDLDQLRVELDKYIGPFKEEHRSTGATLVEQLRSITLEQTKLPRVTEQTSNFKDDAAQLSSEHKKLQVSHKALCDDMSALKSKIEVVEKGTKETTRKHIQLAKDMESLHDHVSKMNISSADTQAPMLTNGSEGARLEQIQTELAEMAQNIATLGEDLGATELTTHDHANAIRTLQEDVPALFKDKLDPFKQAVEQRLETLDRKLEVQSGEVEKLNWLAKAQQAQLGTASRDLHDLKEGNKRLQSTIIQRMATQDAAVLEVTQQLGLKADVITTDNKMDVIRAALRVLQDQYNNITTDELHQRMVHWFVSTYPTAQNLLQDISSLQQDVQRLVQSGNQLQQARANHTSFDARTALAKNEEFGSKLDQQGKQIEKFQSAIAETETLRLGQEEQAKQIESVQAALSGLQSSMHSLNTNSPFAKAEAINVLKTAVDDLGVRVDQGFADAQQARIDTNNELRTSAGNQNDQRVKAEQQIRKSNIDLSNELNAVQESMDTLEKGFANHVAFSKGALGQLREDINTLQENYIEPNKDLFETYSFILVSVAQLQFIVEAMNQKLPGALEFTFKYDLNALALPPPQVEGQKTQNGSASSSGKGKSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAL6</accession>
    <name>A0A9W8YAL6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nuclear distribution protein PAC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Lissencephaly-1 homolog</fullName>
            <shortName evidence="11">LIS-1</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">nudF homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 15" type="primary">LIS1</name>
        <name evidence="11" type="synonym">PAC1</name>
        <name evidence="15" type="ORF">N0V83_004412</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for nuclear migration during vegetative growth as well as development. Required for retrograde early endosome (EE) transport from the hyphal tip. Required for localization of dynein to the mitotic spindle poles. Recruits additional proteins to the dynein complex at SPBs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Self-associates. Interacts with NDL1 and dynein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">Cytoskeleton</location>
            <location evidence="11">Spindle pole</location>
        </subcellularLocation>
        <text evidence="11">Localizes to the plus ends of microtubules at the hyphal tip and the mitotic spindle poles.</text>
    </comment>
    <comment type="domain">
        <text evidence="11">Dimerization mediated by the LisH domain may be required to activate dynein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the WD repeat LIS1/nudF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAL6"/>
    <dbReference type="OrthoDB" id="10264588at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051012">
        <property type="term" value="P:microtubule sliding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023052">
        <property type="term" value="P:signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000342">
        <property type="entry name" value="Nuclear distribution protein PAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.960.30:FF:000002">
        <property type="entry name" value="Platelet-activating factor acetylhydrolase ib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03141">
        <property type="entry name" value="lis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017252">
        <property type="entry name" value="Dynein_regulator_LIS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037190">
        <property type="entry name" value="LIS1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056795">
        <property type="entry name" value="PAC1-like_LisH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050349">
        <property type="entry name" value="WD_LIS1/nudF_dynein_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44129">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24951">
        <property type="entry name" value="LisH_PAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037647">
        <property type="entry name" value="Dynein_regulator_Lis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109925">
        <property type="entry name" value="Lissencephaly-1 protein (Lis-1, PAF-AH alpha) N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 11" id="KW-0813">Transport</keyword>
    <keyword evidence="3 12" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="PAC1-like LisH-like dimerisation" evidence="14">
        <location>
            <begin position="7"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="111"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="153"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="198"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="248"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="352"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="78"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF24951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4371196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="461" mass="50356" checksum="8EAE9561452738D1" modified="2023-11-08" version="1">MSAILTDRQAEELHKAIIAYLGVVNAPKTAEAFRHEANVSAAFDDATRKKYEGLLEKKWTSVVRLQKKVLELEQRNQSLQSELDSTTPTSLLRRNQDPTSWLPRAPARHTLQSHRSPITCVAFHPVFSSLASGSEDTTIKIWDWELGELERTVKGHTKGVLDVDFGGPRGGTLLASCSSDLTIKLWDPSDEYKNIRTLPGHDHSVSAIRFVPSGAAGSPSSGNLLVSASRDKTLRIWDVTTGYCVKTIRGHADWVRDVAPSFDGRWLVSAGNDQTARLWDASSGEAKCTFLGHEHVIECVTIAPPVSYANLASLAGLKKPPPLSSSAEFIATGSRDKTIKIWDGRGTLIKTLAGHDNWIRGLVFHPGGKYLLSASDDKTIRCWDLTQEGRCVKVVTDAHGHFVSCMRWAPNVVKDAPVNGDASNGTTNGVSKKKEEDVAKARIRCVIATGCVDLNVRIFAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAR0</accession>
    <name>A0A9W8YAR0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003800</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAR0"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="151" mass="16321" checksum="5E77DFD069DE2FF0" modified="2023-11-08" version="1">MFRPQHRQKSSSITVLSKDLNPVLRIVLIILVLGTVLAVLVLFRSRQPSAEVPDADMEAQRAAVADEEAETGHARAASQAVNSAAQALDDYAGPSTMMNLSPIHHKPDNNLLPPALPMPVVTPAPRPRTTRQLYRGRPFGFTMDPAGIVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBJ6</accession>
    <name>A0A9W8YBJ6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anaphase-promoting complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_003513</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBJ6"/>
    <dbReference type="OrthoDB" id="2110451at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024789">
        <property type="entry name" value="APC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024790">
        <property type="entry name" value="APC4_long_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13260">
        <property type="entry name" value="ANAPHASE PROMOTING COMPLEX SUBUNIT 4 APC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13260:SF0">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12896">
        <property type="entry name" value="ANAPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="6">
        <location>
            <begin position="30"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 4 long" evidence="7">
        <location>
            <begin position="325"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4373220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="711" mass="79715" checksum="DD34ABAE9E767C08" modified="2023-11-08" version="1">MAIDEPGKERQLLQQAEKILLHPIHPHLISYCPTMDLIALVTSEENLDVYRTNGQRAFGLKRKSEDVKVDALQWEFNGKSIAVSWSDGSTDLISAETGKVTHKDVTLPAPQPQEDSMVEEAPLRVTCMGWGLNFIDVDAVKRRTGLKEKKANGAAATSTDGFGNPTTEDWDAFKDDTTLEDFLQRQPDFQALDIAPDLPDQLAMMDMESLFPKLPAIPLPPALPFMRVAVAASGAFSSQAEVDSLLHSHHLRDHNSVDMFIRCSDRGTIHPSIYDTLETVNVQLPSAWNIESMPLLHASHPYSCTHALLMRMRSAASPRKSQLAFVPLTLNFIPSAGIYLHLIASKTSQLQNFLLYITQTLQRIRTFFKHAQDLPSKFMMNISETLEEKGQGDLVTNLFHIACTGNCPPLVREWLVDELAEQGHKRWDNTVTTSFTTLLALVHENLIPALDRCSIVISRLRGLAQFHDRDWIFTSPISDFSALLEVLKNMRLLAHTVLLYASEEKRQFHAFSKWLRFTIDYEATEPDSQSRAEMENRDPGVDISQVLEYIKHGLTKSDLTPYLRPKEQLGGGGVKNAQAASYEDTKKAIELLKEGGAFKEEALCLEHVLGHLSTGVTGLLRQVSAWQESNIRMESALVLDEYDADEAEGEEEESRVQDMRMMLELTNISPAFPLLPRQHIDLHPALPTATLLNNNHQIINPTTHPPPHPQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBM2</accession>
    <name>A0A9W8YBM2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_004304</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBM2"/>
    <dbReference type="OrthoDB" id="6770063at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF171">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="364"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="398"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="1"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4372529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="432" mass="47640" checksum="E6C30BCE8B64C37A" modified="2023-11-08" version="1">MSILSPLVSSMFTPGIAQIADDLNCSTTAVIGTTTGFVVMLGIGPLCLAPLSETFGRRKLYLTCFSAFTILQIPAALAPNIGFLITVRSIAGFFGSVGIANGGGTISDMFVPSERAGVFGWYLLGPLLGPTLGPLFGGIIVQRLGWRWIFWIMTMVCFVNTLAGYVFLRETYAPLILSRKKAKYEQETAEWDGTKYTYEGEDNRPLGQKVLHSLKRPPKIFAQPIVATMAVYQALLFGTTYSIYTNMQPIYQDGYGFDTEKVGLLYLGPGLGFLFAVWFLVPRIDTVYKTLTARHNDKARPEFRLPLANIGAVFIPISLFWFAWTVEKHTHWFASISATFFYGIGQVMILNCTQNYFIDSFEKYAASAIAAGTVFRSLVGGVIPLLAPGLFEKLGYGWGISTFAFVSLVLAPAPILFYIFGEGLRERFPVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBU2</accession>
    <name>A0A9W8YBU2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004595</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBU2"/>
    <dbReference type="OrthoDB" id="5242705at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021514">
        <property type="entry name" value="DUF3176"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35394">
        <property type="entry name" value="DUF3176 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35394:SF5">
        <property type="entry name" value="DUF3176 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11374">
        <property type="entry name" value="DUF3176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="531"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="632" mass="69744" checksum="814F4AD01F2379DE" modified="2023-11-08" version="1">MPWPAFPDIPIYLEAADERATAKTYGTTPTTPTTPEIAPANLPHGGPTHLHPDHATIPQSSPIDPSPTTTIQTSSDRSIIWPIPEPPPEIQQQQPARRPTLRFFPAERPRVRLGGVPSLQIPQINEKTPINNIHPGLGIVNGPPKPPSIEISPPEKEKSDSPNIAQRIEETLWRYSASGNILKRWLLEVISWLFSAICMAAVIGVLIKLKDEPLTKWALAEKTGLTLNAYISILSKMAGAALILPVSEALGPISPETTPNGTVCGYFFNVAGGEPILMSGYIVEGADNETSFGEALMVRTFPFSDMETKAPIMNGSINFKHISNPILDALIVSASNGTNSIYERKPPVAHECVLSWCVKTIESSYDWGQYSENVTDTYMNRTILPFPWESVPVQLADNSTASVIIYNQNITIERESPRDPTSLTAVSNATYNVDNTTAADVMIIFDSIFPSYYTDHGPSGKPVLRYKDGSDGPSFRDLDFNPWLAPNNLTRHMERMATAMTNVMRSDVDSKDMLEGDAYNKENYVSVRWEWLTLPLGLLFISLIFLAATVVKSALERDRVGVWKTSAYATLLYGLPDEMTKKITRSSSTGTPRAKAKELKVKLQQHGWRVSGNLFSPFAPKPRVNQPPPGWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBU4</accession>
    <name>A0A9W8YBU4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosome maturation protein SDO1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_003665</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Associates with the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SDO1/SBDS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBU4"/>
    <dbReference type="OrthoDB" id="10253092at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1250.10:FF:000001">
        <property type="entry name" value="SBDS, ribosome maturation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1250.10">
        <property type="entry name" value="Ribosome maturation protein SBDS, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.900">
        <property type="entry name" value="SBDS protein C-terminal domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036786">
        <property type="entry name" value="Ribosome_mat_SBDS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002140">
        <property type="entry name" value="Sdo1/SBDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039100">
        <property type="entry name" value="Sdo1/SBDS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046928">
        <property type="entry name" value="SDO1/SBDS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018978">
        <property type="entry name" value="SDO1/SBDS_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037188">
        <property type="entry name" value="Sdo1/SBDS_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="SDO1/SBDS_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00291">
        <property type="entry name" value="RNA_SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927:SF1">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20268">
        <property type="entry name" value="SBDS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09377">
        <property type="entry name" value="SBDS_domain_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01172">
        <property type="entry name" value="SBDS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89895">
        <property type="entry name" value="FYSH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109728">
        <property type="entry name" value="Hypothetical protein AF0491, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS N-terminal" evidence="10">
        <location>
            <begin position="16"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS central" evidence="11">
        <location>
            <begin position="111"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS C-terminal" evidence="12">
        <location>
            <begin position="211"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="142"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF09377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4373370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="306" mass="33434" checksum="65CA238558AFEDAC" modified="2023-11-08" version="1">MPVGINQPSNQIRLTNVSLVRMKKGKKRFEIACYKNKVLEWRNKIEKDLSNVLQIENVFLNVSKGQVAPKSDLEKAFPKKSVEEIIKDILENGELQVGEKERNAELERTKNEVIDIVAGKLVDPKTKRVYTTGMIEKALDQLSSQAAAQQGDKSDKGDKSETAEHGEDKGKAKELPKWTGIVTNKSAKSQALFAMKALIAHQPIPVARMQMKLRITCPTSVLKQAIKTAPKAQAGGGDEKASTGTVKDAILGFMEKVESQDTLGAEWEAVGLVEPGAFKGLNELIEGQTKGRGNVEVLEMAVGADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBZ5</accession>
    <name>A0A9W8YBZ5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial phosphate carrier protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MIR1</name>
        <name evidence="12" type="ORF">N0V83_003593</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBZ5"/>
    <dbReference type="OrthoDB" id="427452at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000024">
        <property type="entry name" value="MIR1p Mitochondrial phosphate carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF12">
        <property type="entry name" value="MITOCHONDRIAL PHOSPHATE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="35"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="133"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="231"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4373299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="329" mass="34205" checksum="B7219EFE0B3F148F" modified="2023-11-08" version="1">MATSPPPTATGSNKLDAAKASVAAHIDPTAPTGVALYSRFAFAGAVCCAVTHGALTPVDVVKTRIQLDPATYNNGMIGGFKKVIANEGAGAVWTGFGPTAAGYFLQGAFKFGGYEFFKQQSINLLGYETASNNRTAVYLASAGAAEFFADIALCPLEATRIRLVSEPTFANGLIGGFGKIFRQEGLGAFYSGFGPILFKQVPYTMTKFVVYEKVSEAIYGVVDKNSTSSGMQTVYNLASGLMAGFAAAIVSQPADTMLSKINKTKGLPGEGTTSRLIKIAKELGLRGSFGGIGARLFMVGTLTAGQFAIYGDIKKALGATGGVEIAKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDE7</accession>
    <name>A0A9W8YDE7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002785</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDE7"/>
    <dbReference type="OrthoDB" id="192832at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02181">
        <property type="entry name" value="GH16_fungal_Lam16A_glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF24">
        <property type="entry name" value="GLYCOSIDASE C21B10.07-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26113">
        <property type="entry name" value="GH16_XgeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040998949" description="GH16 domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="2">
        <location>
            <begin position="49"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="300" mass="31470" checksum="BB37877CC5968F03" modified="2023-11-08" version="1">MSFKSVIIASLPFFWQRATALPAGPGFWANGTSSPTSDVHADAAYALIDTYDGSNWLSKFDVQAISDPTHGFVEYVNAQQAQSLGLLKTQGSQVYMGVDTTSVLNPNGSGRKSVRVQSKTAYNHGLVIADFAHVPGSICGTWPAYWMVGPNWPNQGEIDIYEGVNVNTYDQITLHSSPGCTPTIGAGGETGSRISNTDCGAGGGYNGCGILSNSATSYGTAFNANGGGVYASLWTSSGIKVWYFASSNVPSNIRSGNPDPSTWGTPTANFGGCDFDSKLKDLNIVSYARGKIVALDGKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDJ4</accession>
    <name>A0A9W8YDJ4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phospholipid-translocating ATPase rsb1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">RSB1</name>
        <name evidence="2" type="ORF">N0V83_002015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDJ4"/>
    <dbReference type="OrthoDB" id="2129688at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38119">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38119:SF2">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="451"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="463" mass="52617" checksum="71FC171052824ACE" modified="2023-11-08" version="1">MNQAEDVEVILGHERRYKFHSGTLARNSTLFADMFTEPNAAKLNSRARNAGIKIRWMIELTRLPCEQYPAGCLELVELTPNGERADGRSGLVVNENGRIPQAHKAFAYYEAIFYAFYNKELSIDDTDMTAALSDCYTLLKISDYLGCTSIISKPIEVALLKQGQDLFRAIAAKPSAWVEMAYRIRSESVFRECMIHLVGNWRVHKLSPSTLDQLREIPGLRALIEKYHRALLQKGKDLEIVILSNYPGNMALPADDLPIKREAYAKDILIWMALTFYRHWVGQRLINDKGRHAPDSGYDLYKALGTGGDAYMGKAVTNQFHVKFPMTKKAMNVLENHVLEIKACMKKLVQESGILKWNCQLDCHRFPVKYLTCTEFARADYPWLNADVWAHPRSLAVVKKREYKPGGNDIARQNLDTARMFQGRGASAEMEVDEEEEEGGMVEGSEMEDVQGDHQMAKRARVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE58</accession>
    <name>A0A9W8YE58_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001037</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE58"/>
    <dbReference type="OrthoDB" id="10264588at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15488">
        <property type="entry name" value="Tm-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12030">
        <property type="entry name" value="Uncharacterised protein family UPF0261, NC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12020">
        <property type="entry name" value="Uncharacterised protein family UPF0261, NN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051353">
        <property type="entry name" value="Tobamovirus_resist_UPF0261"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008322">
        <property type="entry name" value="UPF0261"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056778">
        <property type="entry name" value="UPF0261_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044122">
        <property type="entry name" value="UPF0261_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002674">
        <property type="entry name" value="PRK02399.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31862">
        <property type="entry name" value="UPF0261 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G10120)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31862:SF1">
        <property type="entry name" value="UPF0261 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G10120)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06792">
        <property type="entry name" value="UPF0261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23189">
        <property type="entry name" value="UPF0261_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033271">
        <property type="entry name" value="UCP033271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0261" evidence="1">
        <location>
            <begin position="3"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="UPF0261" evidence="2">
        <location>
            <begin position="190"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="418" mass="44662" checksum="F292E91CD7D87B8A" modified="2023-11-08" version="1">MEPTVVLLGTCDTKLEELVHLRQEILRHTVNVTLIDVGRTLTQHDDITISCEELVRDHGAGKDIAELPRGEIIKTMAGYATIAVKSLFEKGSIHAIMAAGGSGGTSLAAEVMRKAVPIGFPKLIVSTVASGDTGPIVEETDITLMYSVVDVAGLNQVLRNILSNAAAAIAGMAHVYVSRRSHADSLTTKKQRVGITMFGVTTPAVDAIRKHLESNYDIETYIFHATGHGGKSMERLIRAGEIDAVLDLTTTEICDHLTGGVMSAGPHRLEAAAKAGIPSIISVGATDMTNFGPMSTVPERYKDRKLYEHNPVVTLMRTSKDEARQVGEFIVQRLRNYAKDANAIQVWLPSGGISAIAVPDGPFADEEADAALFDAIKNGLSGSGVEVVVDQRAINEESFAHDIAEALVAKMGMSRKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE78</accession>
    <name>A0A9W8YE78_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_002295</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE78"/>
    <dbReference type="OrthoDB" id="5284003at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF40">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="3">
        <location>
            <begin position="267"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="83"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="122"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="226"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4375209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="789" mass="87176" checksum="D039B752EA29F7DB" modified="2023-11-08" version="1">MMRGHKQQQPGTVAVAELAPPPPPPPPPSPAPVTVGSHPVGSNAPPLLWDVALCAVSDPSPLWSREIIGSASARPVALFDHNRVPTMSSSPHASQYPPPPDGDAAHYTPIYATAQSLDGPAPRAPYPPPPPPEPPFPKLENINEVLQAQQALHANHAQLLPPHSPASQQPKPNRLRKACDSCSARKVKVGLGAPVLPAMAVRRRGPPNRHAEAVKKRRLNHADPSSAGSSPQSPTNAAHALAQLQSSHPAQLSAETICPLPTMNALIDDFFMYIHPLCPFPHEPSFREAWERREDFSNPSFLALLASMIAALVSSFPRKPRLHLKSQTRHEYPSHLSLVDKCREVCSQARGPGYLDRPSLNVYDACTSYFLGLTGAYVFQWRQMRLYIAESLTIIRSLGLHKSESPGYTYLGSVPSAWGSSGPNFDGSRDFKLDHITEQIGRRVFWTVFVGVRTLHQLGASFGELSIPPATPTELLPPLPEEVDDVHIFPNEIQPQQIGVVSLMAGFNANVRIYLSYSTLATAEMAFGVDEIFDWGRQQRIFEESLQRCKQALENIPAVLKIQTKPGRAGGFIQQRQPYYPPMPEFSDMRNPALNSYNGAQSHDTHRYEIQKANIYASHLATRSYLVEKYFLQLEKYHTTKSQVALQESPVAIVAGLDKFSARHPVDSEALERTMSDEREQVVKDLLVVLGSIDMVNMEPNGDSFTQKIRSIASTLLEVPKERRGSVALQHQDYLYTFLNILGKLERASPEGSDPASGPVDEETELRLWADLKEHQLKFQEQGGVYGFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEZ1</accession>
    <name>A0A9W8YEZ1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF952 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEZ1"/>
    <dbReference type="OrthoDB" id="3335358at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.170.20">
        <property type="entry name" value="Protein of unknown function DUF952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009297">
        <property type="entry name" value="DUF952"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34129">
        <property type="entry name" value="BLR1139 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34129:SF1">
        <property type="entry name" value="DUF952 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06108">
        <property type="entry name" value="DUF952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="14647" checksum="DEBE56A779F563B8" modified="2023-11-08" version="1">MPAPSPLPTHLYKILPSAPPSPLPERLPLSDLDRNDGYIHLSTSEQVPGTADKFFGSVSELWLLKIEYEVLAAGTDGDGKVKTEDKPQVKWEEVGRGYFAHLYGGDLGRGNVKSVEKVEKRGTWAASLSLLEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF63</accession>
    <name>A0A9W8YF63_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">General stress protein FMN-binding split barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002352</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF63"/>
    <dbReference type="OrthoDB" id="434253at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052917">
        <property type="entry name" value="Stress-Dev_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038725">
        <property type="entry name" value="YdaG_split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34818">
        <property type="entry name" value="PROTEIN BLI-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34818:SF1">
        <property type="entry name" value="PROTEIN BLI-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16242">
        <property type="entry name" value="Pyrid_ox_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="General stress protein FMN-binding split barrel" evidence="2">
        <location>
            <begin position="29"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="204" mass="22034" checksum="430B78FC859BB611" modified="2023-11-08" version="1">MSSFSNTDTGSKNADPYTQKNLQEPSLKEKVEDLLNFVDNTKFCLLTTQTVDSDLLASRAMALAAKEGNGFDLLFHTNTESGKTDDLKEHKSVNIGFINSSGEWASISGHATVETDRAVVKKHYSPALKAWIGDLGDGTHDGGPEDPRIGIIRVKASTAQYAVSRRTAVGGFVELAKGIATGEAPSVNKLRHISEAEVQQWKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFA9</accession>
    <name>A0A9W8YFA9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_002427</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFA9"/>
    <dbReference type="OrthoDB" id="1925334at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF104">
        <property type="entry name" value="CYTOCHROME B2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00557">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="6">
        <location>
            <begin position="7"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="7">
        <location>
            <begin position="55"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4375341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="365" mass="39967" checksum="5B416377513F5099" modified="2023-11-08" version="1">MATNDQLKTVTRWETSRHNSKDSCWIILHGFVYDVTDFLDSHPGGKQTILEHAGYDATEAFEPFHSRDTLEKYLKPSFRPRVLKPIKGVDISTTLLDTRFSAPFFISPAGGGKLAHGTGEILMTKAAAKHGILHFVCNNAGCTQQEMADARDPSQVLFWQIYAMKDLEVTKREIKQAIASGYKGFALTVDAIYVGKRERDMRLSIAALKSESDDQDEEDSGLSGGISVSRPAVFTEFDWITAVKWLRQITELPIAIKGVQCWEDAALCMHHGVHPWLSNHGGRQLEGAPSAVETLLEIRQNCPAVFDKCEVIVDGGITRGTDIVKALALGAKGVGLGRAFLYALVFGEVDASGLPYAAAVVRACL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGW6</accession>
    <name>A0A9W8YGW6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGW6"/>
    <dbReference type="OrthoDB" id="10251048at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000069">
        <property type="entry name" value="HAD-superfamily subfamily IIA hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050324">
        <property type="entry name" value="CDP-alcohol_PTase-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006353">
        <property type="entry name" value="HAD-SF_hydro_IIA_CECR5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01456">
        <property type="entry name" value="CECR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269:SF51">
        <property type="entry name" value="HYPOTHETICAL HAD-SUPERFAMILY HYDROLASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="496" mass="54778" checksum="21FE7BA7CAA5E8F5" modified="2023-11-08" version="1">MPGFDDSDFGVNTPLQGTKSRDVNQAPSNNLNVGSPTLAIPSEDENGTVNSIEVPATRSSISDASHFMHNLSLSPSMKDRRGSRNSFGTSLPIPRSKRQSRLSSVHYPEGQPVRPGMPPIQASRDILASQMQDLSGEKVRAAKDMAFVFDIDGVLVHGDRLIPEGQRVLEILNGDNELGIKIPHIFLTNGSGKPEEARVAQLSKILKNPISTEQFIQSHTPMRALADYYKTVLVVGGEGYKCREVAEQYGFQDIVVPNDIIASDPTIAPYRVFTEEERASSRPRDFTKTNIEAIMVFSDSRDYATDMQIIMDLLRSENGRLGTMAKDPVSQRIPIYFSQGDMLCPTEHPFPRMSQGAFRIGLEAMYKSLTGEDLERVVYGKPELATYKYADEVISSWMETIHNDEKLPKNIYMIGDNPASDIIGGNMYGWNTCLVRTGVFQGGDNDEENPASFGVFVNVLEAVKTAIKKELGVDFKFNFNEAINPVTHGNGVSAIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH86</accession>
    <name>A0A9W8YH86_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">Peroxisomal membrane protein PEX14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">Peroxin-14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PEX14</name>
        <name evidence="13" type="ORF">N0V83_000885</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 receptor. The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm. Mechanistically, PEX5 receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9 10">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peroxin-14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH86"/>
    <dbReference type="OrthoDB" id="5549158at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000489">
        <property type="entry name" value="Putative peroxisomal membrane anchor protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025655">
        <property type="entry name" value="PEX14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006785">
        <property type="entry name" value="Pex14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058:SF0">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04695">
        <property type="entry name" value="Pex14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="3 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peroxisome membrane anchor protein Pex14p N-terminal" evidence="12">
        <location>
            <begin position="3"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="49"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="242"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="267"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="347"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="358"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4378055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="369" mass="39943" checksum="4EEB0BD770335369" modified="2023-11-08" version="1">MVREDLITSAISFLQDPSVAGAPLEKRIAFLQSKNLTQEEVDISLARAAEDPSQPHPHRHRPHHLAMPTDSPHQRQPMEVILLKDTGNHHPHQSIPPKRDWRDYFIMATVMGGVSYGLYFTAKRYILPLIQPPTQPQLEQDKASVDESFKRAFDLLEQLNTDTSALKASEEARTTRLDNALGEVESVLASLKESSKRQGDDNRRIEDDVRGLRDLIPKALDAQKESTDNRLRELSTELKSLKTLVGNRMGSGTARPPTATPSYYGAGQSQSPAPTPNVNGTPAASTPAPQPTSTPAPSEQTNGTSINDAQGSSSASTPSTEKPAPSSFRGLPGGRAAIPAWQMAAQKKTEEAKKDTSESGTAVDASSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHM7</accession>
    <name>A0A9W8YHM7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000934</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHM7"/>
    <dbReference type="OrthoDB" id="2139939at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044688">
        <property type="entry name" value="SCI-1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34117">
        <property type="entry name" value="STYLE CELL-CYCLE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34117:SF1">
        <property type="entry name" value="STYLE CELL-CYCLE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4378102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="210" mass="24527" checksum="9A681ED7EBF67264" modified="2023-11-08" version="1">MKKTVQSYSARSPQAPRKRASPSYAAADQASEEQSDDDFGPAPPTHIARHLGHGPTIPKLDDLTYRNELRDEDRTQNQADYVDDLRYERKSDRKAQKERLEELVPRADPGSRERQLEKKRETTSTLQSFRDAKESGDVEVAEADLMGDDGIDMYKKKKRDEQRKMTEREIRREEIARAREEERNEKLSQRRAKEGKTMEFLRQLAQERYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHR4</accession>
    <name>A0A9W8YHR4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000578</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHR4"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="140" mass="15123" checksum="648ABC33A5F47902" modified="2023-11-08" version="1">MPKTLAALPSSQYATLFDVVLGKLLDLVSPLVAAPATVALPAPAPSTVALVRSGRVDFHLTDCKTEGWRAAGRGAEIAGRTGELRHGLHTNNLLESRGAAVRSWGKAWNIEAMAAARWTRKARRRPSTTRWYFFGGSRNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIX2</accession>
    <name>A0A9W8YIX2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_000557</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIX2"/>
    <dbReference type="OrthoDB" id="5319341at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF3">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="3"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="25"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4377728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="533" mass="60323" checksum="91252ADD12CC7516" modified="2023-11-08" version="1">MPRRVKCDELRPRCATCSKSDRECMYAPATDSPADPKPSASDSARTSTPPLQDDALATSSSPSVAKKSVTADIDPQLFVNSPELLFDSPLTNVASVPSPNSAPLEWYDLLAEDAINNIDKYNLNLDIDRTCLSRRQSPVPEEETIDPVLDPSGQDRDVINSSDEQWNCPENIHLTNDQLVLFQHYISVVGPILDLYDSSKQLTTTLPRMAVRNVGVLKSLLAVAARHLALISEPQIHQLNLGEQIGRLESNASDANSFQNSLLQVATQYYYETLQYLSQNLLYPSYSKSREIIATALLISTYEMFDASGQYSNGAWERHLRGIFWIQRSQNNNGESHDPLRRAAWWCWIRQDTWVAFREGRRVLTIWRPTKRLMDLNANELCLRILYICGRCVDFAANERKYDMITRIDQGSKLLQALEDWHHALPSSFQPIYKVQLPEPGNLFAPIWIHPPSHAAAVQTFHFARIIVLINQPSMGGMEDFRARQRFLDESVETVCGMAMMSQEKDLASAFADFQALYAGKPHVSLIYYISRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIY5</accession>
    <name>A0A9W8YIY5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_000210</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIY5"/>
    <dbReference type="OrthoDB" id="5290825at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015798">
        <property type="term" value="P:myo-inositol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015791">
        <property type="term" value="P:polyol transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000100">
        <property type="entry name" value="MFS sugar transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF14">
        <property type="entry name" value="SUGAR TRANSPORTER, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="426"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="456"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="483"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="553"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="122"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4377385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="642" mass="71638" checksum="476EB7ABE9D28119" modified="2023-11-08" version="1">MAESEKDTKIEQVDNGHHERKGSIFENEQVRRESIARLTANLEGEIRNPLVDIPREQLLADVKAFARENQLEDVEPLLVKGALVAQSPALFEELEELDDDNRRAIREEVTNRFKLPKLLYFTIILNSIAAAIQGWDQTGSNGANLTFGDAFGIPDSGDKCTAAGTCERNQWIIGFINSCPYIAISLFCAWISDPVNELLGRKGTILVAAVFSVLAPIGSACTQHWGQLAACRILLGVGMGLKEVTVPVFSAEIVPASVRGGLVMSWQIWTAFGIFLGTVANLAVMNTGKISWRLQLGSAFIPAVPLLLGVWFVPESPRWLMKKKRYAKAYKSFLRLRNTPLQAARDLYYTHALLEQEEVLVREAGLNPKSNFFTRFGELFTIPRVRRATQASGIAMIAQQMCGINIIAFYSSSIFAEGGASVKESLIASFGFGLVNFVFAWPAVWTIDTFGRRGLLLFTFPNMFWTLLAAGMCYYIPSGSSAHLGLIAFFVYLFGAFYSPGEGPVPFTYSAEVFPLSHREVGMSWAVATNNFWASVLSLTLPRMLKAMKPQGVFGFYAGLNILALIMIFLWMPETKQRTLEELDYIFAVPTRTHMNYQVKQNLPWWFKTYILRRKGLSKPQLYKIGNQDAHGKVSPRGEKQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJY2</accession>
    <name>A0A9W8YJY2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJY2"/>
    <dbReference type="OrthoDB" id="10324960at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="191" mass="20473" checksum="44DC466C492C8E9F" modified="2023-11-08" version="1">MAQRVTLSDIHDGLPFDSGYMSDVPDAPDAPLRKAKPSIFTLDPSSSAHGLDITTVAFDDTNWESSSNNKPSTSLKVKQPKGFPIIPLESTMARARLEAKVKLETKATAKVNERGGASSRVKKRRAPVKGKAVVVDGRGRTRAARKALLAESAEAGLPLADFKFEEGNAVGIKEEKESDHGGVLAFDAEMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CGH0</accession>
    <name>A0A9W9CGH0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_010356</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CGH0"/>
    <dbReference type="OrthoDB" id="3789331at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4362263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="163" mass="18738" checksum="0DD879D245499878" modified="2023-11-08" version="1">MNAQEALRKAKERKSEFFYSVFGDGAIDDAVNECTNNEANWHRSADDAWTIQLSSELIRIADGMRANYISIREERKVDSACWIVARTLQYHVTTNTEYTGRDDALRHVFKLIHTVNKTIDSDSDVIRLKANIENRVTEKLGDAKAKELHKEKTYLSLPEDVDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CGY7</accession>
    <name>A0A9W9CGY7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010205</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CGY7"/>
    <dbReference type="OrthoDB" id="1263307at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052897">
        <property type="entry name" value="Sec-Metab_Biosynth_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37017:SF13">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37017">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="10"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="273" mass="29320" checksum="4A5924572B2FE0AE" modified="2023-11-08" version="1">MPTTTHSTELVIVPGSFSSSKLYDVMVDPLRAKGYTAHVLDPPCYPESYKKGIPSPSMYDDAAFVNAFVTKLADEGKDIVLMAHSYGGVPTSQSLKGVTKKEREEQGKKGGVVRAAYITSIVPKIEENLATTISAAGDQGGAPPIDVDEDGWMSQPQPELTAKMVFNSLSPERGTAEAAKFGKHASVSFGGALTHAGYKDVPVSYFFCEDDLCVAPLVQQNGIDAIEESWKGTPREGKKVDVTRVKCDHVPLLSAPAELEKWVEEVIAKGADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH54</accession>
    <name>A0A9W9CH54_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2OG-Fe dioxygenase-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009695</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH54"/>
    <dbReference type="OrthoDB" id="5307791at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018724">
        <property type="entry name" value="2OG-Fe_dioxygenase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10014">
        <property type="entry name" value="2OG-Fe_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4363402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="321" mass="36538" checksum="06ECA95A97125368" modified="2023-11-08" version="1">MPYINGQKARRRGPHILNVLERRYTAQFYESIASIMALRHTYLQNKFIFVPGENLIPILKGLGAREDDFESVKEISDLTGPDPTLDYRTVTYGRFCIDPETRSIRRLETQPYTLTEQEDYKRHDSGIPRTFDEAPTDMQGNTVVQALMLFKSLILQGVPVTPRDGLDYSSPAWICTMFNARTHTSKNNGIFGEPALEGVHSDGSDHTMTVFLGAQNMRPDSAVTFMHDNEETTGVQIWETNPTLIRARVHHRHFLDTLMFVDHDFKHSVTSVHQLDAAQPACRDMLVVFTRRPKLEGHVSGYADSMELHSTGPLHLPMWLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHE0</accession>
    <name>A0A9W9CHE0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trs31_1</name>
        <name evidence="8" type="ORF">N0V83_010418</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Plays a key role in the late stages of endoplasmic reticulum to Golgi traffic.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the multisubunit TRAPP (transport protein particle) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus</location>
            <location evidence="7">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHE0"/>
    <dbReference type="OrthoDB" id="10254842at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990070">
        <property type="term" value="C:TRAPPI protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14943">
        <property type="entry name" value="TRAPPC5_Trs31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1380.20:FF:000007">
        <property type="entry name" value="Trafficking protein particle complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016696">
        <property type="entry name" value="TRAPP-I_su5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20902">
        <property type="entry name" value="41-2 PROTEIN ANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20902:SF0">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017479">
        <property type="entry name" value="TRAPP_I_complex_Trs31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6 7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR017479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4362325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="217" mass="23766" checksum="4F0C9FAA3995FB16" modified="2023-11-08" version="1">MSGTPSQGPGLRYPSNKKSIYDRNLNRSKNAELSRAAFAYLFIEMIAYAQKGAKDVGDLEQRLNSQGYPIGLRLLDLLLSRSSNPLASIRPTRILPLLQFIAQTLYRHLFGRPADALERSGTDPGQYMLFDNEPMVNQYISLPKELSSLNCAAFVAGVIEGVCDGAGFPTEGVTAHSVGEQDEGKDAKGMWPGKTVFLIKFKPEVLEREEILGRGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHF8</accession>
    <name>A0A9W9CHF8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta-galactosidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009984</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHF8"/>
    <dbReference type="OrthoDB" id="1657402at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000065">
        <property type="entry name" value="Beta-galactosidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.20.10">
        <property type="entry name" value="Beta-galactosidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.390.10">
        <property type="entry name" value="Beta-galactosidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018954">
        <property type="entry name" value="Betagal_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037110">
        <property type="entry name" value="Betagal_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025972">
        <property type="entry name" value="BetaGal_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036833">
        <property type="entry name" value="BetaGal_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025300">
        <property type="entry name" value="BetaGal_jelly_roll_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13364">
        <property type="entry name" value="BetaGal_ABD2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10435">
        <property type="entry name" value="BetaGal_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13363">
        <property type="entry name" value="BetaGal_dom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01029">
        <property type="entry name" value="BetaGal_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117100">
        <property type="entry name" value="Beta-galactosidase LacA, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-galactosidase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4363687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="509" mass="55201" checksum="84110B9538F398D8" modified="2023-11-08" version="1">MTLNGRQSRWVVTDYAIGNETLLYSSAEIATYGLFEHSVIVFYLKEGQTGEFAFKSHGNISYNIFGTHSDFTSASMNGTSYSRFTYTQGKGTTIVQFSNGIIAYLLDIPTAYSFFAAATTNDPNVTPDRQIFVIGPYLVRSTSVSDGVVTILGDNSNATAIEVYAGANVDTIIWNGKKLPTRCTEYGALTALIDGTEDRKVTLPELSSFRAADSSPEIQATYDDSKWVVADKMVTLSQVKPLTLPVLFSSDYKFYTGAKIYRGYFSGKTATSLNITVQGGAAAGWNAWLNGQSIGYHPGNASLWSTNSLLSFKNITLKEEGNVITVITDYTGHDQTSTGPSGAENPRGILGAQLLSANNTNVAFDQWKIQGNAGGERNIDPVRGPMNEGGLYGERLGWHLPGFDTGDWEDESPVEVGVQGAGIKWFTTTFDLNIDEDLDVPLGVEFGAPTGTIARILLFVNGLVCSITSLMMTLTRLAGINTASIYRTLALKRASQSHQAFSTRPERTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHH8</accession>
    <name>A0A9W9CHH8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009385</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHH8"/>
    <dbReference type="OrthoDB" id="3777260at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="158"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="223"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="282"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="459" mass="51769" checksum="788B8E639C75E8A6" modified="2023-11-08" version="1">MRAKKPYAPASLSPKTNSLQSASSTPIRRLLLSPLATPFQAPESIEVWSRKQQLASTPDSTQLVAEDHTQDLLILDAEHIDQDTVGDEFHAKIKLQAPSPPIQSSPLISEPRRPAHIGRLRPSSPVESFHSAVSAHQPAPESVVRLTKHVWDSMGTDLQMLTAQKRALEAKLARLEHENELLRSEEKERNLEVQIGKLRYQNEINRDQKASMGRSLNLQEVEIKNLQLLTDDLAKKLSEAESDLNKYKGATTNLEDHMKHTVLERDDVLIAQTSTEEFRAQIQDLAATLAEKERIVTDLRQKHLEEQMKVTDLEDEIEALRSKGNQDDIGGLEKLREKATQYDLLRKQVKGTEQQLQLSQERLARVANEGDMLRGAAHLVAPNPNSKLPKHAIACFECYSLNLPCDSKSRCQSCTDRNSKCVRWRCSLKQKLGECPLAPCKFPHDGNGWLILQTARPEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHL0</accession>
    <name>A0A9W9CHL0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010539</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHL0"/>
    <dbReference type="OrthoDB" id="202203at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735:SF5">
        <property type="entry name" value="FAD_NAD(P)-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="65"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="440" mass="47875" checksum="6D669636C6158AFD" modified="2023-11-08" version="1">MTALNDTNDTNPKSSQSRLIKTVNSARVFLKLLGVFLPYSIRLLRIRIGTIYHNLTYKPVDSPKNVIVVGGSFAGVQVANWLANAVPLGHRVVLVEKNSHFNYLFAFPRYAVVAGYERGAFIPYEDILGDAVKKGSLLRIRSEALTATATQLLLADGQAIDYAYLIIATGSSQSPPAKWISTGREGACDEMRSTQRSVSKASRIVVLGSGAVGVEIAADIKTYFPDKYVTLFSSRNVVMPQFGRKLQIDVADTLKQIGVVVRYNTRPKALPDRKSIQLPDGTTEEYDFVLSCTGQTPNSALLNALAPQAISKQTGRILVKPTLQLQPADDFSFPNIFALGDVAEHGGPRMARAVFMQAGIVRDNILGLIKGRSPSQQYLPHVWIEGAIQVTVGKNKSFAYMPGDNGTDMLFAGAGYPDDFNIAHGWRLVGANYPGHGKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHQ0</accession>
    <name>A0A9W9CHQ0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHQ0"/>
    <dbReference type="OrthoDB" id="5144514at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006771">
        <property type="entry name" value="CetA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36195">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G01990)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36195:SF6">
        <property type="entry name" value="SECRETED THAUMATIN-LIKE PROTEIN CALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04681">
        <property type="entry name" value="Bys1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040928090" evidence="2">
        <location>
            <begin position="20"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="206"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="288" mass="31457" checksum="AE5F1F7EF92E932A" modified="2023-11-08" version="1">MQLITLISAASAMVATASATSAIDLNKDCDTSQPYARIINRCEYDVYLWSVLKGNGCPDDEMVTLKSGETYQENWRDPVNGAGVSIKMSKTQQCKPNDIVQFEYFLETSNSAFAYNYLDVSYVDCTGDDCPTKNEGYYLVAGDQQGKYASSSDNTWCPILSCNDAASCAKMSYILPDDVQTKTCDLKQNAEFYLCGGEAPTGDYDSTPASSSEQASSSTPSPTSSAEESSTQEYKVKAAAVTDAPEIKAAEGQKTKTQYEVVYATVYEYVNAKRHAHGHARRHQAFHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHX2</accession>
    <name>A0A9W9CHX2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vacuolar protein-sorting-associated protein 36</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ESCRT-II complex subunit VPS36</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">VPS36</name>
        <name evidence="12" type="ORF">N0V83_010326</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the endosomal sorting complex required for transport II (ESCRT-II).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the VPS36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHX2"/>
    <dbReference type="OrthoDB" id="271448at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000165">
        <property type="entry name" value="Vacuolar protein sorting protein (Vps36)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000527">
        <property type="entry name" value="Vacuolar protein sorting protein (Vps36), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021648">
        <property type="entry name" value="GLUE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040608">
        <property type="entry name" value="Snf8/Vps36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037855">
        <property type="entry name" value="Vps36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031558">
        <property type="entry name" value="Vps36-NZF-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13128">
        <property type="entry name" value="VACUOLAR PROTEIN-SORTING-ASSOCIATED PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13128:SF12">
        <property type="entry name" value="VACUOLAR PROTEIN-SORTING-ASSOCIATED PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04157">
        <property type="entry name" value="EAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16988">
        <property type="entry name" value="Vps36-NZF-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11605">
        <property type="entry name" value="Vps36_ESCRT-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51495">
        <property type="entry name" value="GLUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GLUE N-terminal" evidence="11">
        <location>
            <begin position="6"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="100"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="297"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="193"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4363206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="592" mass="64281" checksum="FF6496B578F2EB79" modified="2023-11-08" version="1">MFLHQIDLTTALRPSLLPEETLLFVQDAVGLYEGKFKIAQYQHGHAYLTSHRACYVDHGEPRKNSVAIFLKDVDRTEFYAGFLKSHAKITLYPKPLKQISRGSSVPPSSHIPSPGTPPRHGSPAPVPSASATWVCPICSYSNPVPSNFDPALASRIPVPPCLACGIKPPTVLVVKAAIAAMSNRPAPGPPPKNTNNGAPAPTSSGSMLSCPRCTFQNHPSLSSCEICGASLMTAVDKRLDLSKDHVRSESPGPSLASPSQSEVPECIKISFRGGGEKIFYERLKNAMVQRKWLLQSAPPIPKPRPSSGAFDDNYSNKSDKSEAETERPRVVGIAGLERRGLEQRQNNEAMIGSAFEDLEALMTSAKEIIALAEQFASQANLGTNGNSEANALASQSASALGLVTTRDMLGTGSGSESLYVSELSRNLAEWLTDDTRGILRKEGGIMTLVDLWAVFNRARGGVELVSPSDFEKAARMWDTLKLPVRLRQFKSGLLVVQGRDRTDEKTIASLLAWLKSLHQEPPVADVTWDWQAYGRGVTAQETAERFGWSVGVATEELEMAEEAGALCREQGIDGFRFWENWLVNMAEAPTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI35</accession>
    <name>A0A9W9CI35_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008582</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4364966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI35"/>
    <dbReference type="OrthoDB" id="3794564at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="266" mass="29027" checksum="323D089C446B1713" modified="2023-11-08" version="1">MQQTHFKKPAPKPHTTNTSTNINTTRPSTSSSSLNEQPPTATTTTTTTPNTNITLTTKLNFLPLLHDIRRKLGADYTFYRDFLDGVLRAHRLSTTSQSPSSIPSSYSEDTSAYFEQVAQLLQGREEDVAFSHQGVLFLLGEMGVKIPVAGREADEVAGGEEGEEEMSAPGFRITSAADEEDKGEEWEEVEDDDDDDEEEDEDVIPEYPQGIPSILPSSPSDDDMSPPAPSVPRPGTARIMSKAATPLKLGVDPIFFTTMASAEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI98</accession>
    <name>A0A9W9CI98_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">diacylglycerol O-acyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_009925</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Sterol O-acyltransferase that catalyzes the formation of stery esters.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.20" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI98"/>
    <dbReference type="OrthoDB" id="10039049at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014371">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408:SF7">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="207"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="379"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="426"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="490"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="523"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="556"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4363629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="605" mass="68014" checksum="68EB1B40AAA0CBCA" modified="2023-11-08" version="1">MRRPGLLRLGGPSHNADADATLDAPNGNTADCRLQTALQLLPDPPFANRRFRASYQSSLPPPSSEDVQDVSKGHSSSINGFVDFTPSSRLAMATITQMATGIAFDNVPEASVSRYRGKTTEQKTNSTAKRIRDTKYRHVFATHSSARTSCLSQDAESSPSFVGFRNLMILVLIVSNLRLMIVNLQKYGVLICISCHDYRRSDVITGLLLYLLVPAHLFVTYLIELAAAWQANRAYTRISGEKEGDDTPDKYAAALRSFNSTWYIVAFFHSVNAMANLYIATKYVYYDIYHPGIGTLCEFHAVIVFLKCASYALTNRDLRHAYLHPKQADPLPELYKSCPYPQNINISNLCYFWWAPTLVYQPVYPRTAHIRWPFVFKRLAELAGLSIVIWIASAQYAAPLLQNSLDKMLTLNLTSIAERVMKLSTISVFCWLCGFFALFQSALNALAEIMRFGDREFYGDWWNVASIRAYWTTWNKPVTNFMRRHIYSPLVGRGCPPALAQILVFLFSGVLHELLVGVPTHNVIGVAFAGMVFQIPLIALTDVVQKVSWVKGRVAGNMIFWVSFCLVGQPLAALLYFFAWQAKYGSVSKTMNLKESPAMSIFLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIU9</accession>
    <name>A0A9W9CIU9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009187</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIU9"/>
    <dbReference type="OrthoDB" id="3692269at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="208"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="255"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="445" mass="50004" checksum="DCAF9AB2BCD503DF" modified="2023-11-08" version="1">MKSGAYYQGRWAPPRTVDIIEYEPRAILSTAAEARQQPPQATQVQAIEDHRRTLISQQRPQMRLAHGQKQQHSAHESHLATDQNVRTAVQSYPSSPSNDMQQMAAPYMPIANMGTGNAMSPALHRPPYGIGQTPWATANSPLATVSNAPQDTSILRIPTANMYTAGQVAAQVAAQITAQRGQPATFPSAQRHPVPAVLPPRSVFDSRQQQVTSCTTVSTINSTSHMPTLSRQIPPQPSQSTQGRKRRANEPPEGTAQAQKRQATAASNPPLHTGRYRTPDIMQRLSTAMAASNAATEVASPVSNSQAQLTREQQAYKNELFESHRLAEEQRLERQRWLARKEELRKDPSALFRHYHEYIEYFPLGYGEYKSQYHSRLLANQRMPFEPESDLGVAITYAKEHWDVYMEYPKDVTRATEVARKKAQKQEHDARQQWMIKTKSGRAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJB7</accession>
    <name>A0A9W9CJB7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008309</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJB7"/>
    <dbReference type="OrthoDB" id="10249419at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35006:SF3">
        <property type="entry name" value="GLYOXALASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_3G06020)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35006">
        <property type="entry name" value="GLYOXALASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14830)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="394"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="485"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="441"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="497"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="535"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="553"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="657" mass="69983" checksum="AA13F12E0D16A954" modified="2023-11-08" version="1">MPVSHIGLTSGNQIGLGIRDADFFLCQETPGVKAGAAHIAFTAPSTTAVRNFYTAALTAGGRPNGAPATRCEEDGHFNAAVLDFDGNSIEVVFRGEPDIRDDGTVIEHSRVITWQRTVAESYRDDRSVVSARTSASKPAATEVMSQAASVVSKAPSAASKPGSMARSVSEPITVPQTASTSDSGDRAAKKIIGTLLGAAAGAAVAYAMVRSEQDSAKKESDFSAFMEAKNTVKAAAGYLAQASSQPTQPMQDPQPTIETALPPQSVHRNIDAQSYHSNTPSRTGSIYAPRQIEAAPPSYYSPTQISVPRTRVAEPRAIEYVPAQSVAPSKAHSRLSVQRSMTNPEMLTLEKARSVASTLRPESVMSVSKPKSVAHSVAPSSLISSFVPDVTRRSSEGSVASHHSSRSRAKSSHTHASRHTSHSRSSRKEVDESSPSPPAPASRAPTNAPSKVASKAASLVGSILGRDAKSSASKNDDFIDDFEIEELTEDDMDTVVPSDSISNAGTSSRRSHRSHRSHKSKKHDDDESVVSKHSSTSKHSKRSHRSHKSRSHHTSEDSADEYEKRKRSSRPSVVSEPSDASTVKPIKSASKVGSRKDSVTSGQYDGLFDEVQYGSGSVATMPVRGITPSMISAAGKNKNKTVVNYNHAQRMRAFEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJI1</accession>
    <name>A0A9W9CJI1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_008208</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJI1"/>
    <dbReference type="OrthoDB" id="5086500at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052374">
        <property type="entry name" value="SERAC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48182">
        <property type="entry name" value="PROTEIN SERAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48182:SF2">
        <property type="entry name" value="PROTEIN SERAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="8">
        <location>
            <begin position="80"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4365588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="306" mass="33968" checksum="590D93FDFCA54C5B" modified="2023-11-08" version="1">MDEPTGLVELYRGENPIVDIVAVHGLNGHPFNTWTTRKTKKFWLQDSDLLPKSLSRARVLTFGYNATVAALLGKTSSDRIMQHAHTLVAELVADRELEDASQRPIIFICHSLGGIVVKRALAYSASRTSKLVHHLHSVFVSTYAVLFLGTPQNGSGKASLASTGSRMINALTPSKIVDTESQLADALHPGSEVLQNITDMFAPLMKNFRIYFFWEQEKTNLGTTLSYIVEETSAAPILDNTERAGLPYVHADMAKFESRNSPGYRLVVAALLRYSRDAPELISVRWIQAAEMLRTKRQNEAAELVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJX6</accession>
    <name>A0A9W9CJX6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WD40 repeat-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_007996</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJX6"/>
    <dbReference type="OrthoDB" id="1068471at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052234">
        <property type="entry name" value="U5_snRNP_Component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006:SF1">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="62"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="105"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="147"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="196"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="231"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="281"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="323"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4366360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="38780" checksum="B2241CF968C81E85" modified="2023-11-08" version="1">MSGEKRPAADSFGSSQLVKRAKSDANLGGSSAVAVANGAAQNGALIQAISRTGALQNPVMELTGHSREVFAARFDPTGQYIASGSMDRSILLWRSTGSCENYGILSGHKQAVLDLHWSRDSQVLFSASADMHLASWDVETGERIRRHFGHEEVINCMDVSKRGTEILVSGSDDGYIGIWDTRTKDAVAFIPTDFPITAICLAEAGNELFTGGIDNDIKAWDMRKQAVTYTLLGHTDTVTSLQISPDNQTLLSNAHDSTVRTWDVRPFAPADRRIRTYDGAPTGQERNLLKASWDSKGEKIAAGSGDQSVAIWDVQTGKLLNKLPGHRGAVNDVRFHPKGEPILVSASSDRTLMVGELGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKJ8</accession>
    <name>A0A9W9CKJ8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006742</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKJ8"/>
    <dbReference type="OrthoDB" id="3799259at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="532"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="397"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="578" mass="65295" checksum="9890438FC6991D89" modified="2023-11-08" version="1">MSSLKEFFSRGDKDKLSPELTRALELLELPDIDALKKRFFSTDAFLKPWAVFRDSVLLLDPNSMPCATVIHSSEVTGGITRLEILWFYNQFDAGSLRYDPRADRNTFDYTPDRTIHGVIDKSKWGVREHEKLLYAGLLQEFKVGKRINPLGFEYFELLHICTAWEEVFVPIINVIRASYNVKGRRHYRRLALFIEDLRPSRLAFPEGNVGMPEGGPISPTPFRVLSAPRKTISSEEPTPDKSGSSGGVLTKKDYDETEKELRVTYPQYGGLVERPKFRFNMDVWLADQRIRAGRRKVVQGHQEANESSRLPEGHPSPSTPKRRASLQVSRSPIKWFNHSTRRSSSLSTSKKLSEEEKSPLLGEARDLYNKDDAAPGSLSPEQGSPHVSKSPMKRFSNRLSRSFSLSTPKKVYKDEPESLPQGRLYNTEDAAYGSLSLDQKRIKEAKEQNVSSPASSDTVIRRPRHRALNEHREASEESLYTSIRNTNPFNEDTPGKGKAPQLRAEPGDPGYMPMGQRGVTQAVITAEEVRAIRARNRQGPPKPLPYVPALHAMPGDPHPEVPPRHIPWPGFESDDEGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKM2</accession>
    <name>A0A9W9CKM2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_007663</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-binding monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKM2"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050775">
        <property type="entry name" value="FAD-binding_Monooxygenases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43098:SF2">
        <property type="entry name" value="FAD-BINDING MONOOXYGENASE AUSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43098">
        <property type="entry name" value="L-ORNITHINE N(5)-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4367133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="654" mass="72216" checksum="6B9A53562F0D7506" modified="2023-11-08" version="1">MSTVIESNESAIPPEQKYFVEAAKRKRTEGTKQFEQLHFSQNEKLRHLADDIYADHAALDRLPVPIKSGDHVKFVVLGAGIGGIVQAIKLIKQGFSAEQILLVETAGGIGGTWYWNRYPGLHCDVEAYCYLPLLEETGYMPKQKYSSSVEIRTYLEDTVKKFGLDSRILFRSQATGLKWDEGLKAWKANLKTRRGPEGKEQTQISVDTDFVFIASGLFPHPQVPKVSGLADFGGPMLHTARWNYDITGGSSDTAFPEMEKLKGKRVGYIGTGATAIQAVPQLAKYAKELYIFQRTPSQVNTRGQRDTDPVEWREKIAAKSGWQKDRMENFAEHLAGHLQPGTDDLVDDGWTQLQAYCALIGSNRFGPIAPDKAQEHIGALMALDSEHNSKARERIQQIVQDKETAHKLTPWYPTWCKRPTFSDMYLEAFNNDNVHLIDTDGRGIDSVTPNSVVANGQDYPIDILILSTGYRSPTSGGDPGSRSGVEIIGRNGRRLATKWDEQGQTTLHGIFTNGFPNLFFQSAVQAAATANYSHVLTVLSEHIASIVATAYKRSSSSADKQSKVVIEPAAAAEDAWGMQIAQGAAFFSGVAICTPGYLNLEGEALQMPPPDDHVAMMKKAKAAIWYKGLVDFTRILEGWRKDGKLEGVEISVNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLA0</accession>
    <name>A0A9W9CLA0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">D-serine dehydratase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_006035</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DSD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLA0"/>
    <dbReference type="OrthoDB" id="20198at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008721">
        <property type="term" value="F:D-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036088">
        <property type="term" value="P:D-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06819">
        <property type="entry name" value="PLPDE_III_LS_D-TA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.20">
        <property type="entry name" value="D-serine dehydratase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051466">
        <property type="entry name" value="D-amino_acid_metab_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026956">
        <property type="entry name" value="D-ser_dehydrat-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042208">
        <property type="entry name" value="D-ser_dehydrat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28004:SF2">
        <property type="entry name" value="D-SERINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28004">
        <property type="entry name" value="ZGC:162816-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14031">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01119">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-serine dehydratase-like" evidence="3">
        <location>
            <begin position="266"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4368953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="374" mass="40052" checksum="2A856A44D78956BF" modified="2023-11-08" version="1">MPPYSPKIGDSLASLDTPSMIVDLDLMESNLAKLMSQLLPTGINIRPHLKTTKSALLAKRLVAAGAKGGCVAKLSEAEVMCAKGFSDLLITCEIIGAPKVQRLVALFREYRSIRIVVDSEEGAKAIDEALGQSGIGEPITTLIDLDVGLHRTGVQPGEAALKLARFVASRKNLRLIGLQGYEGHLQHIHGLEGRKKLCLESMAILTQTAEALRNDGHAIEVVTTGGTGTAEFCVTVPGVTEVQPGSFLFMDTDYRNAVGSHNYSNSLTILSTVISKQGPKTVTIDAGLKSLTTDSGLAECKTKGYVYGVLGDEHGSLTWEDEAGSGGLELKVGDRVEMIPSHIDPTINLHDVYYAHRKGVIEEIWPVDARGKVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM17</accession>
    <name>A0A9W9CM17_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cleft lip and palate associated transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_005388</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLPTM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM17"/>
    <dbReference type="OrthoDB" id="378564at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008429">
        <property type="entry name" value="CLPTM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21347">
        <property type="entry name" value="CLEFT LIP AND PALATE ASSOCIATED TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21347:SF0">
        <property type="entry name" value="LIPID SCRAMBLASE CLPTM1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05602">
        <property type="entry name" value="CLPTM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="399"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="486"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="511"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="609"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="628"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4369626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="667" mass="75346" checksum="5D14BC0CB68D748E" modified="2023-11-08" version="1">MPEAQAQPAVEEKSTFRTIAQSVGIFLLIQGGMKYLMPQAAKAPAANTAQDGTPAVVHTPNTNIVIPAWENRPRQLEPGTEISPVPWNIAPMWPTGTELDISMYISPSVAMPALKKMPKDSLLIEEKNFVFGDYKQKREIHTEFTVPKEVQHNATLLAHFYVAQSGSVLDPTQPGYDTAKAYHFIRPLTVYHPKKKIAKTRNLLSDMPERDAQETEAEKNAPKVIASYYHPNFTLSLIPDAGTLAYPNLHPGPRRYIQLEASGARDASGQHGWYYPILFTNTFWQLKKHMIELNDTVTTLPLNVNLNNLAHWKYNLFASVDENLNANARAAAEGKSTPGGGDGSEMEMFKEILIDSNSYLLAITAVVSVFHMIFEMLAFKNDVQHWRKKKDNIGTSVRTILANVFMQAVIFLYLLDNNENTSWMILFGQGMGMAIEAWKITKSVNVRVRPTAPGSLLPYSIVFEDKHVLSETEKKTEEYDQIAFRYLYMVAVPLLIAYAIYSLMYDTHKSWYSFIITTLVGSVYAYGFLMMVPSLYINYRLQSVAHMPGRAMTYKFLNTFIDDLFAFTIKMPTLHRLATLRDDVIFFVYLFQTWKYKVDYGRVNEFGQGGDDEEVEEKDANRPLAAHPDGDKSLKVPDADKLAKVEKEKAAQATGSQTKGQAKQRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM69</accession>
    <name>A0A9W9CM69_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM69"/>
    <dbReference type="OrthoDB" id="3660917at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="198" mass="22693" checksum="7BDB915AA30D104F" modified="2023-11-08" version="1">MSSPTAQLPSAQDRSPNDRDQGPDQFELRGLAQNKDGEGGITRPAQVETGKDQYNALVRSLRRPADRKCIHMICTDGVYRLLHYLPTPPDQPTDYEIYDAKPMSPEMIKAYLDTLPWSQAKEDRFRGADGRTIPQEQWWNPPPGIVHPMRSKNERDEVMRKYREEKEQLEQKIARGEISPEEPVACASIKSDYDLRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM76</accession>
    <name>A0A9W9CM76_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycine zipper 2TM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM76"/>
    <dbReference type="OrthoDB" id="3797879at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="34942" checksum="1BA89A215098A9DD" modified="2023-11-08" version="1">MAAQDYYLGSQAAQELPGHAPPNLYPQPQYPQPPPQPYAQQNYNASNYTNTPPPSYSLHPQQSTPQQQLQAYDPQQQYPAKVPQALGAPYPQTPPPAQYYPPPPQQKNSYLGAPLQPIRSHSQPARVRFADQESDRSTDLGSESDSDNSPQRRRRHHRHRSSNDRDRDLDYSVRERERGHRHSSSKIDKESHKNRDTFLGAGAGTILGDAIFPGLGTAAGLLLGGYGGRKYAERSKSEDSLRHHRHRDAFRDGKEEGRQEREYRRRKEEYDDSDSDYKRSSKGHRRRPGENGWDAETATYKSGWAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMB9</accession>
    <name>A0A9W9CMB9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cation-independent mannose-6-phosphate receptor CI-MPR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">MRL1</name>
        <name evidence="13" type="ORF">N0V83_005749</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMB9"/>
    <dbReference type="OrthoDB" id="4504960at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028927">
        <property type="entry name" value="Man-6-P_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071:SF0">
        <property type="entry name" value="MANNOSE 6-PHOSPHATE RECEPTOR-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02157">
        <property type="entry name" value="Man-6-P_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040764913" evidence="11">
        <location>
            <begin position="20"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="12">
        <location>
            <begin position="26"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4369985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="238" mass="26274" checksum="2476613221B3833B" modified="2023-11-08" version="1">MISLLPSVLLVLLSQYATAASDKPLKPCTVISPSTERFFDLNPLQRFPPSEDGKKKKEGDEGSWHARGYDYGANFTINFCGPVVEELDEVQDLDKSVWRNVSAFYERGGKQWAIGDPDDDDSDNDSDKTTARRKTTVISLLCDSDPLAKSSISFVAAVDDCTYFFEGRSPFACGGVHQETQALGPGGVFGVIALIAVLVYLVGGCVYQRTVMHQRGWRQLPNYAMWAGIWQFFSGFAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP47</accession>
    <name>A0A9W9CP47_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA polymerase kappa</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_002902</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP47"/>
    <dbReference type="OrthoDB" id="1747274at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03586">
        <property type="entry name" value="PolY_Pol_IV_kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.810:FF:000001">
        <property type="entry name" value="DNA polymerase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.810:FF:000003">
        <property type="entry name" value="DNA polymerase kappa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000014">
        <property type="entry name" value="DNA polymerase kappa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.100:FF:000010">
        <property type="entry name" value="DNA-directed polymerase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000039">
        <property type="entry name" value="Polymerase (DNA directed) kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.60:FF:000012">
        <property type="entry name" value="Putative DNA-directed polymerase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="entry name" value="DNA polymerase, Y-family, little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017961">
        <property type="entry name" value="DNA_pol_Y-fam_little_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050116">
        <property type="entry name" value="DNA_polymerase-Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022880">
        <property type="entry name" value="DNApol_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002677">
        <property type="entry name" value="PRK02406.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11076:SF33">
        <property type="entry name" value="DNA POLYMERASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11076">
        <property type="entry name" value="DNA REPAIR POLYMERASE UMUC / TRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11799">
        <property type="entry name" value="IMS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="Lesion bypass DNA polymerase (Y-family), little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UmuC" evidence="3">
        <location>
            <begin position="145"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="539"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="603"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="552"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4374161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="654" mass="73578" checksum="81EBB405A08B5985" modified="2023-11-08" version="1">MPAAAASTTMSTPKDRAGANKNHQQPSIAPAMSEPSRIPADRPQPSRTPSNDHDSLKYHLLGPSLTKAGQDTVDQQKVSEVIYNASKGSKFFNNEEVKDKNLTEKITRILAKRRQLEKVDVTSDLRRADDYIAELELTRDLSQVVVHIDCDAFYAAVEELDRPELKEVPMAVGKGVLTTCNYHARRFGCRSGMAGFVAMKLCPQLICVPMNFAKYSAKAEEVREVLALYDPGFESASCDEAYLNLTKYCQDHNMDPQEAVSQLRAEVHEKAKITVSAGIAANAKLAKICSNKNKPNGQFYLPSDRQTIMEFMKTMPTRKVNGIGRVFERELDAIGVKTCGDIYAHRAYLSQLFGQKALQFLMQCYLGLGRTQIQRAEDYERKSVGTETTFKELQSPDALRDKLRHVAEELEGDLKRTEYKGRTLCIKIKLHTYEVHTRQTTPPFAVHKADDLYKYGLQMLEKLMKEIPNMRLRLMGLRVTHIVSTKKPGIDFFGRVKASSTVPKNTTATNGGDWEVWPEEEFEEAARQERNDEMNELEKLSQEHEEEEQQRTGDTNSGAEQEQVSEEQWTCPICTLQQSPDDASFNAHIDFCLSKQTIKEAVKSTAPPTLMKQSSVSSKTTQKKGKRGRPKNEPGASDQQAREKRRAFFSLGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPF1</accession>
    <name>A0A9W9CPF1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001872</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPF1"/>
    <dbReference type="OrthoDB" id="5169850at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4374796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="286" mass="32146" checksum="A0CBAA9F09A5CFFA" modified="2023-11-08" version="1">MSTLTNTKTPNPTYNPSALKSRYEIRKLEHEHLAWASALLVHSNAFHSTIWPKIYPENVSGRMFEMFAGVDYLVLHQINSGISFGVFDTEYQFKTEEAKKQGGKLLWDSKEPSIEQEQGLKAEGDRLLAQMDFPLVSVALSYDGFNPLDMDKMGSLLALLPHFGLVYHILGSQDPRDPASWQPTGPRQVLMRNATSTRHDYEGEGIMSGLARWLMREAAGKGYRGIQIECLADAVTHVWSTAEAPFKGSVVTEFECETWKDEEGKVAFAPAKQRVTKCYVDLQPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ59</accession>
    <name>A0A9W9CQ59_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ59"/>
    <dbReference type="OrthoDB" id="3793565at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040896156" evidence="2">
        <location>
            <begin position="24"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="311"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="350" mass="38789" checksum="D563DD32C77FCC51" modified="2023-11-08" version="1">MIFTSKHLAAASLLTVLFSTSIASQCFLTRDVEACPANTWAQEYNCLMAQNDLKTIMDCVQINQKSDFEYPICRAPDDGVDDDLEQYYYQSSNKKGLWCTTPRNILIFGPATKQNYWKLGIQGAIQACPENDPDFHNCLCMQNVKQQSLVVLAQYFRQVRNQDLSCPGSLTKRTNFLDDVAKAPHLHRVREMTSNHNRIQARAYDYDFDIDDFGQPVYDQGQVGYRLPYFDQYIWNGEAAQRCYIHPTVNDIVSCRTWLLETPVTSALSVQPTKANYSQAFTAASSVMPTATIAPETTDIPSEQIVASGEAPGNGSAAPTATAGTTSGGNTLDLSSLTAVLSIAAFLFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQN3</accession>
    <name>A0A9W9CQN3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="12">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">tmk1</name>
        <name evidence="14" type="ORF">N0V83_002289</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Proline-directed serine/threonine-protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="12">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="12"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQN3"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075018">
        <property type="term" value="P:positive regulation of appressorium formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07849">
        <property type="entry name" value="STKc_ERK1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000040">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000073">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 12 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="20"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4375203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="352" mass="40817" checksum="0402593A1756C7D0" modified="2023-11-08" version="1">MPPAGSGSSRKISFNVSEQYDIQDVVGEGAYGVVCSALHKPSGQKVAIKKITPFDHSMFCLRTLREMKLLRYFNHENIISILDIQKPRNYETFTEVYLIQELMETDMHRVIRTQELSDDHCQYFIYQTLRALKAMHSANVLHRDLKPSNLLLNANCDLKVCDFGLARSAASTEDNSGFMTEYVATRWYRAPEIMLTFKEYTKAIDVWSVGCILAEMLSGKPLFPGKDYHHQLTLILDVLGTPTMEDYYGIKSRRAREYIRSLPFKKKIPWKAMFPKTNDLALDLLERLLAFNPVKRITVEDALKHPYLEPYHDPDDEPTADPIPEEFFDFDKNKDNLTKEQLKLLIYQEIMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQX6</accession>
    <name>A0A9W9CQX6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Histone acetyltransferase type B subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">HAT2</name>
        <name evidence="8" type="ORF">N0V83_000215</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQX6"/>
    <dbReference type="OrthoDB" id="427795at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="7">
        <location>
            <begin position="27"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="182"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="279"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="323"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="433" mass="49467" checksum="2CC683B90620B811" modified="2023-11-08" version="1">MDDNMSDAPDSREMEDQEQVEHKIINEEYKIWKKNSVFLYDMLYGRALEWPTLTTQWLPDKKPVEGTNMSQHRVILGTHTSNQAQNYLQIAHCEIPDFRVPDLSELNEERGEIGGHGNAKRPFDFKIVQKINHPGEVNKARYQPQNPDIIASLCVDGKVLIFDRTKHPLQPKDDTVKFEAELVGHNKEGFGLSWSPLNEGHLVTGNEDTTVKTWDIKTGFSKSNKTISPTATYNVHSATVNDVQYHPIHNFLIGTASDDLTWQIIDTRMESHKKALYRKEAHEDAVNCVSFHPEFEMTMATGSADKTVGIWDLRNFEKKLHSLQSHRGDVIGLQWHPQDAAILASSSYDRRICFWDLSKVGDEQTDEEADDGPPELLFMHGGFTNRICDFDWNKNDPWVMMGAAEDNQLQIFRPSRKLVEPLKTTVNHGEVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQY0</accession>
    <name>A0A9W9CQY0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQY0"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="105" mass="11713" checksum="585334BEEC0437E5" modified="2023-11-08" version="1">MEVPICVLFVITFMTAGIGGSTKKLAFGASYQLGYTVGNIIGPQTYRKEDAPDYYTAKYTMLAFLVFTMILIAVMGIIHWSWNKKRDKQDALDAQSMLILSNCMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR19</accession>
    <name>A0A9W9CR19_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Cyclase 1 superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR19"/>
    <dbReference type="OrthoDB" id="5396at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.50">
        <property type="entry name" value="Putative cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007325">
        <property type="entry name" value="KFase/CYL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037175">
        <property type="entry name" value="KFase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34861">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34861:SF11">
        <property type="entry name" value="CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04199">
        <property type="entry name" value="Cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102198">
        <property type="entry name" value="Putative cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="341" mass="37372" checksum="D15A37670F4A9EC6" modified="2023-11-08" version="1">MSPPSRPLQRPPLDKNGPPGNAWGLYGAADELGALNMITPSTVKAAAQEIQTGDRVSLDWTLNLPSYPSFGRPSFGWKMKNKTHPDGTKRIVNDDHLDINTQSSSQWDGFRHYGYQKAARYYGGRTQGDIESSEVIGIDRVAHSGGITARGVILDYPRYLEKKGKQSVNALSENSINADTLREMLKETGVQPRDGDLLLLRTGFTRDYEKLGEQERKAVAERAPTFLGVESTKETLRWIWESGFVAVAGDAPSFEMSPLVGAHNKAGGIWKGESWEDEMQGGGLLHQWLLGGWGVMIGEMWDLERLCEKAVELGRATCFVSSVPLKVPGGVASPPNAVAIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRG8</accession>
    <name>A0A9W9CRG8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">DSC E3 ubiquitin ligase complex subunit A</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Defective for SREBP cleavage protein A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">RING-type E3 ubiquitin transferase dscA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">N0V83_001553</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalytic component of the DSC E3 ubiquitin ligase complex which is required for the srbA transcriptional activator proteolytic cleavage to release the soluble transcription factor from the membrane in low oxygen or sterol conditions. Required for growth during hypoxia and triazole drug susceptibility, as well as for virulence in a murine model of invasive pulmonary aspergillosis (IPA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 26">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the DSC E3 ubiquitin ligase complex composed of dscA, dscB, dscC and dscD.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRG8"/>
    <dbReference type="OrthoDB" id="9984778at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044695">
        <property type="term" value="C:Dsc E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000626">
        <property type="entry name" value="Transmembrane ubiquitin ligase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021319">
        <property type="entry name" value="DUF2921"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763:SF162">
        <property type="entry name" value="TRANSMEMBRANE E3 UBIQUITIN-PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11145">
        <property type="entry name" value="DUF2921"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 22" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040939055" description="DSC E3 ubiquitin ligase complex subunit A" evidence="22">
        <location>
            <begin position="26"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="417"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="444"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="559"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="586"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="649"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="23">
        <location>
            <begin position="747"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="478"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="704"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="492"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="512"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="537"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="707"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00071072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00077885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ4376270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="817" mass="92252" checksum="9E900B896258A36D" modified="2023-11-08" version="1">MEVRRDPRSFLFFIILLLLINSPEPQTTTYNARARYEELIEREYYQLDLLNRTSYGDFDAKKDKWLNVTGLRDEDGFAWELLDPVRQRAKEQSERVLGDRWRSIIDGTLEDEGHDIPVYKNLSGYVQGEWVRSPLSRVQHPSDMGNSSMIPDNPFAHLVDFDRNLTGMGGPLRLHFTELEDKMRRNNNKTVSEISAKVVIGDDDSFGGNWWEFVAHGVHFPNSGTTVLTTTSDRYAGIFALPHLQTSEHLYSSSQKFLNWTIHEAIQRQESRVFPLWNPWTSSAEGNNEGLFQGHHCEFVLYLQEFPSQHSVDMEWLEHELRYPTGAPVTQHAQFSMSMVGFSPDCGFVIESKGPPGFPPSEAIHLVGSKAEDYNNRARHSIIAFSVALAFQLWFLIKQMKETATPSMRSRVSFYTIAMMALGDGFTFLILIFMYLFLTTSQLALYSIAFMALFSVLTHLRFLMDIWSVQAAERARLDRQQASPAPAPTPQPAASNPTPASSDNDEGLPLPATAPRPRPPTPIIIAPDQDDPSEDMTPTPGNNTTTTTNTNPRAELGALYSRFCLLLIVVFFITIQFATARAAYRSIYFNTISFLYLSFWVPQIYRNVMRNCRKALRWDYVAGTSVVRLAPVAYFYLKEDNVLFAKTDWTGFAVLVGWVWVQLLALVSQEVLGPRFFVKEGWAPPAYDYHPILREDEEGATMPLHVTTSTSPGSPSTTALDASSSSAEESTKSSAESKNKGRKVFDCSICAQDIEVPVIPAGAEEASVAGMGSTSMILQRRQYMVTPCRHVFHTACLEGWMRYRLMCPNCRESLPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRK6</accession>
    <name>A0A9W9CRK6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001777</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRK6"/>
    <dbReference type="OrthoDB" id="3694819at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="172" mass="18991" checksum="D3567E7230BF7D7B" modified="2023-11-08" version="1">MGQHPPRHKDEKTKEGAESPSTAWWARIVSSWYTQASDKSRSEGVEVGRGEMWQAKSELAAVLSQQKHAVKADEAVTLARQVLSERESMLGPMHQETLESAWLLGSVLEKKGGKEKDASSLYERAYKGAREVLGDGHPDTKDYLGDVERLKKGGEVVSASTSHVQALQDLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CS55</accession>
    <name>A0A9W9CS55_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Integral membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000613</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CS55"/>
    <dbReference type="OrthoDB" id="2603at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="369"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="49288" checksum="453C1D89396F0854" modified="2023-11-08" version="1">MSLQSTPEGRTVPVQSSLDHKEMPSHSADPTPMTNSSDSPKDSPLKRNAAFHPGHEPLKHEHHLQLEKSRVTGPLSFLNPTSAHYRHESQPDVRYKWTSRNNRKGRHAIAIDPQQPGSADSKHSTPRPTSSTSSIAHGIWRMLTYYPFWDISFDIAYVFTIGSVIWVINAFFVFLPLIRPASEFGTEELYGGGITAFVGATVFEVGSFLLMAEALNENQSGCFGWALHRAVSHKEEEGGEGAKLEPSKSHCSHHHSNKRNLVGIGHAAEPRKEVGENGRSWVWWPSREELRTRYLHSFGFLASFSQLIGASIFWVSGLTALPGIYDRMSRPVTIIFYWTPQVLGGSGFIISGTLFMLETQSKWWKPAFGTLGWWIGFWNLIGGIGFTMCPAFGYDENSWAQYQASCSTFWGSWAFLIGSVIQWYESLEKFPVEVEKAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSL7</accession>
    <name>A0A9W9CSL7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial zinc maintenance protein 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MZM1</name>
        <name evidence="9" type="ORF">N0V83_000946</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Assembly factor required for Rieske Fe-S protein RIP1 incorporation into the cytochrome b-c1 (CIII) complex. Functions as a chaperone, binding to this subunit within the mitochondrial matrix and stabilizing it prior to its translocation and insertion into the late CIII dimeric intermediate within the mitochondrial inner membrane. Modulates the mitochondrial matrix zinc pool.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with RIP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I LYR family. MZM1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSL7"/>
    <dbReference type="OrthoDB" id="529194at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20267">
        <property type="entry name" value="Complex1_LYR_LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045298">
        <property type="entry name" value="Complex1_LYR_LYRM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050435">
        <property type="entry name" value="MZM1/LYRM7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46749">
        <property type="entry name" value="COMPLEX III ASSEMBLY FACTOR LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46749:SF1">
        <property type="entry name" value="COMPLEX III ASSEMBLY FACTOR LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4378114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="101" mass="11145" checksum="A2001185C838158C" modified="2023-11-08" version="1">MALVAYRNLLRSARIAFQGSEEDSQKAHNATGDMPTLFAARAEARKNFEQNRNLSAGSEELSKSITHAEEVAKFLRENVVQGQATEGDKYSMCCDFASNVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY52</accession>
    <name>A0A9W8XY52_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY52"/>
    <dbReference type="OrthoDB" id="3762311at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="326" mass="36972" checksum="3FB94D1E6B94C01C" modified="2023-11-08" version="1">MVSSLRRATNATKSAHASAWNTVFVDQKVSKKTGGKNVTAMPVDDINILDWTYEDRQKLVSKGNRVRIFVGEDFVVDMSKPLFRATSTKGHEMLKGGNIKLPSDTDKAGVARLVNHLETVIQFPRPARLVTGMPMSAALSICAAAALLGMDKYVTHVYKKCEALLRMDPPTYEDLDAIVAFSNQHKRLYSIVVNELAVRVWDDTIPDADVFNDYLLKNPILDSAIKDINEKYATKQRIEAEREERRLRYEETRALREEQQKRDNEHAKATMAREKASWAEKKAKAAALEKSCQQKILATTPKDRKFTTEERLHWLRIKGKQPPKGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYA6</accession>
    <name>A0A9W8XYA6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_010682</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYA6"/>
    <dbReference type="OrthoDB" id="5397557at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4362588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="365" mass="42665" checksum="A1616B9CDC26F94C" modified="2023-11-08" version="1">MLQYILSFCDPSTLFQLMHVSFTTRQEAKKLFWSDPTTRYIVDGQWLLAGGHPRHTNHDLEALTHMQYIEVDFTFGASVFVTEWEDGRYYTEIREGEDQRAAFWMALRRRFPRVTDVVLTEGTSKRLGTPPDKGTRLATGSFDGISLSVSQLQWYNNTSVCPASRYLWRWNRSSSTWELTDTSWNPHRITPPVRKYFGPVGAYQRYDHIDMDLAELEYARENHTIQATEAYYVHIRQPPCVCPFPQCGMQFEQPGEWVAHYSQAYIRSRTHDGRVPLPPWESLRSAFARHDASLELKRQRLSEEMARMQAAWGEPGSSERSTASQQFLQQLRNDPLYAGEITPEESEIWFRYQRDMNGQEHPPIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ63</accession>
    <name>A0A9W8XZ63_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peroxisomal membrane protein PEX17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009869</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ63"/>
    <dbReference type="OrthoDB" id="2357318at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055334">
        <property type="entry name" value="PEX8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39214">
        <property type="entry name" value="MICROBODY (PEROXISOME) BIOGENESIS PROTEIN PEROXIN 8 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39214:SF1">
        <property type="entry name" value="MICROBODY (PEROXISOME) BIOGENESIS PROTEIN PEROXIN 8 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26001">
        <property type="entry name" value="Pex8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4363573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="654" mass="72148" checksum="FBB1983AE85E890A" modified="2023-11-08" version="1">MPVDRLLSTLLRSLQTYTDQQDTPRLLGTASSLLTTLGNPHNLSLLTSHILTAPALWDRPEGVRTPLRLLSLFHSAVTTIINHHNDVRYNKAPKPQPGQIPTGGGLPLDDWIRAIVAGADERSQRWKHLVVLGGLLIGIGSLEEQGMDLYWASAMRKRVETALVRATNLALIEVRERSEADGLGGQTITLVLNHAFGCIADAERMQIDYDLLLPVLIGTAFYSNEGFQSAYFLGGLDLDVRVTQVGKLDWSALSSSYRQVEGIMNRPLVASMGPLSRLIAHSVEHVKNPWIIQTMMDDLASFSRALTTQWRQIKFSDVDPAEEAAHLEEEALHRTTPQLWNLLKSALFATTIILRGVFTRTLGDSALAGDAIAPLLASQALQTLRHLYFITSRLDLYFLNTAEHFTLVLSPATNEDLLVTSAVPYLAAGENRQMLPIFEAAHSVMLAVLSAPQSAEITAKHLPFYIDALFSVFPHNLSPRQFRLAFKTLMRVTAPPSTLSASHPDLPATLLELVFHRALSAPTHALPPDEVSLALQGSDAPPPALSEQAVLALTLIDALPFLPIPMLEEWMPLTAELLNQINDHSMRESVKARYWEVLVSGEMDAERSGVAVLWWTTRGGRELVLFGRESVDEQMGMMSGALPVEERNIKESKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZN3</accession>
    <name>A0A9W8XZN3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ML-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_009511</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transient receptor potential (TRP) ion channel family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZN3"/>
    <dbReference type="OrthoDB" id="5312224at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010308">
        <property type="entry name" value="TRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040241">
        <property type="entry name" value="TRP_Flc/Pkd2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032800">
        <property type="entry name" value="TRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_7G01610)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145:SF6">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_7G01610)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06011">
        <property type="entry name" value="TRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14558">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01320">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="364"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="409"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="446"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="498"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="524"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="556"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="587"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="ML-like" evidence="9">
        <location>
            <begin position="46"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="713"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="841"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="883"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1131"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="713"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="813"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="953"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1027"/>
            <end position="1037"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4364056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1155" mass="125742" checksum="730286530D8C6E33" modified="2023-11-08" version="1">MAVEIPTLQPTQPPPRSRRKRRTMPALGAFPTVLLLLVFASFLPRASAVFLNFENCLEQVYLPNKVPYLQFTPMFVWASFNTTDPTHTLNVTVYGDVSGQTATGDYPAPGDDNWSDPNKTIGKIPNVDPSGNHFTTLFHNYRVLTYSAYSSPPVQFCQSLVNGTCPLGPSFSKNTSDPYTLPAISVEHDFSSTYSFTTWAATIRILQGNDDAQPLSCVSANITPALGQKLSDAIRYMPAAVLALVAVATIFAATCSPWGTSNVFRWTSNYGRDEDLLRLVTPGFGDCLQYIQFVVLAGSLSLNYPGFFQPVVSKASWSVLLFNQSFVSHGNGSRSLVDGVYFANGTYGLTRLGQYVGMTRERDIWACMAIFLLVLIGIVILLTQLGFLGRWLSRQLTKTQDGDLTGKNWSFTAGNIVRIVFNYFMLPVVSISLFQLVVGPRSPGSVVAAAVILLILVAVVAIWIFWFIFTTRPRAHLFDDLPTVLTYGPLYNTYSDDAAPFAFIPVLLTVIRGIAFGAIQPSGIAQIIILAICEVILILTLHAFRPFQSNTSMNAYHTFFSVVRLTCILLSVAFVPSLAVSEAPKGWIGYAILLLHAIVLVFGFFLNSLQTIIEVAARLGGVGADQRGGLAAVFGKRQLSKRTAHRTQRSSLNSNAAMLAHDENKNIQLMDSRSRSLSASSAVLLNGPYDRDSTRASVGFDQFSGDYNMMGTSPSTPGTNATPFNFLGGSSNPSSRRTTMGTTLDAADPYYRPPRPRKQTMDSIRPEVQSRGPAGSADIADAPYSDNADLEAGDVGEGPSRTSGTPAPAYLRMNRDDSDPNLERRNNTDYSVRESDFYYGLRGPALSSQPTRKLKTGPADPMSPVSSATGWFKSLNLLGGRKKEKSKGFEVVRSTRMPPQMMVVDEDDEESPEVPQEPYRDSPDTPSPPRNKIPPGVASAGALAGERRRSTDSESDISDEEYDPGYRVSALAPTLGPIETGGGIELPSRIGSRASRVTQQQGPPSRAPTIPRKNSRRTRSTEAAILPSSNRLSTVIDSASPRHSQHLQPTPSGLPIRLPFGSTEPSPSPERSPERSAASSIYRNDGISDLNVPPPRIGAQGDRPLSTGYVQHHVTKDSLHSDGYEAGNRLETSAEFVSRSRSGSTQRSERSQRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZP2</accession>
    <name>A0A9W8XZP2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">XPG-I domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010473</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZP2"/>
    <dbReference type="OrthoDB" id="2959108at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09870">
        <property type="entry name" value="PIN_YEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF62">
        <property type="entry name" value="XPG-I DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG-I" evidence="2">
        <location>
            <begin position="55"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="479"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="634"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="721" mass="80673" checksum="5F5D051A46AF33D5" modified="2023-11-08" version="1">MGIEKLMFYRICRLLTLNIQLIFVFDGPGRPWKRNKRGQGKIDYEKRRLLQEVLRCFDIPYHEAPGEAEAECARLQILGLVDAVWSQDSDCLMFGCTLWIHDDRVAKEKGNNDRSKENTKKNSKSVRVVKAPQMAEKLGLDREGLVLFAMLVGGDYKVSGLAGCGSATALHAVKAGLGKTLCQCQNQRDCSAWSTQLADFIGTRARSKYIHIPGDFPDYKILQKYYRPKVSSDETLLSISRLNLSHDRGIKEVKLLEVTSSRFNIWGRKYMDWVGPVLLTRHLARRGPVLPREVVHNIKLTKRRAKKTDDQPATRCLERKLTFSPFGVTSLQRADFEGDRLGYWNGDMTNLFDPGYRVDCEIPEYWLQKTLPLDVLGPPPPEPKRRTQKRKQQTDGDAQDLGTTASTKRKRKQTDNDQVTGQVPSRASSSKLPVTVRDPQASVTPIKRRKQPTRTGETLLIELSDSEDEDTLRLPSKVHSRKLTSTRSAASEIVDLGSPEPSEDDEHLSIHQQEDTDTPSRAPFHRDGLLIHMIEREERDLQLALRMSLQGQQEAMSPSRSHLVPNRVHARKHSPGVANRPVFSKAPISIPAISPRKAFSHHLDDLTSDPTRTVTSATPVKSTDWSYSSPAVAVSTTPGSTPNAANMTTSKDDVPNTSASESTKLKDIRVARLRHFAPSIPTSNVNETFTTSPSSRITPSKVVKPTVRVPAGVDCIDLTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZR6</accession>
    <name>A0A9W8XZR6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009498</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZR6"/>
    <dbReference type="OrthoDB" id="5343383at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4364043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="266" mass="30449" checksum="796016FAF1F59D4A" modified="2023-11-08" version="1">MPSFSDVSYSREATVAAIRDYYRFLVSMYLDASNVLEPPEGGWPSIPPNGWENFDKTDEVIALLRELPYLRQGSDPYQINGAPYTVFADWQFTDEDIDGQDLKEWSELDPDEAEIPAHIVGLTAPSDKSPAMLLDTELGVIYWYECPRGVKGGEFQEIEGDPYDWVDDDLIPENQVEWRAGSGIWPIETFFKMLKANFQKLNFVPIGPREVQDVWVARDEYAKETQFAVQNIYRKQGWPNMARFNKQACSAEVKTLLEERAPSLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y031</accession>
    <name>A0A9W8Y031_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">L-2-hydroxyglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="7">1.1.99.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_008994</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(S)-2-hydroxyglutarate + A = 2-oxoglutarate + AH2</text>
            <dbReference type="Rhea" id="RHEA:21252"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:16782"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="EC" id="1.1.99.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the L2HGDH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.2" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y031"/>
    <dbReference type="OrthoDB" id="498204at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047545">
        <property type="term" value="F:(S)-2-hydroxyglutarate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104:SF4">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="9">
        <location>
            <begin position="8"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4364400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="340" mass="36886" checksum="4C7FEC91CBC15BA3" modified="2023-11-08" version="1">MVLWGRRRVIHAGLYYGPDSLKTRLCLKGKRMLYELCQQHSIPHRNTGKWLVAQDATQMEALDKVHSFASTIGVPTNFISKDEAQRREPAVRAEAGALESPTTGIVDSHSLMQFLEGAFESHGGILALHSPVTHIAPIDSGRSGWEITTTDPSSSSNDNTTTITAETLINSAGLHAVAISNMILPPSRHKKAYYAKGSYFSYASSQPKTKTLVYPAPIPGHAGLGTHLTLDLSGRIRFGPDVQWTDSPTDYTPNTSDLARAIEDIKSYLPGVDGSAIAPDYVGIRPKLGRLSATSGGDFQDFYIRREDGVQGFVNCLAIESPGLTSCLAIAEEVEGLLYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y038</accession>
    <name>A0A9W8Y038_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010825</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y038"/>
    <dbReference type="OrthoDB" id="3596006at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4361884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="244" mass="27074" checksum="FA86E3A2B0693E08" modified="2023-11-08" version="1">MAVIWGLDLREMQWGKFKSSYMWNNEYHMRRTKFIIYQLAMILCVVSESLGTAALSDYVDQQDFVAKHNPNATVHNDNYVGIASFNIFVGIYVATIFGSAFFFDLFWPERHESPAVKMAWRICSILACLLTLACALAYTDIVAKESAYVTGTNAAETQKLLAEYGGSPLSTYLLWHSLAHIDAYGPKSGHARARDDVAKPVDEKPASLAESTDGAALPQPGHTHARPEGFHDRNEAANQQHSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y044</accession>
    <name>A0A9W8Y044_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_009607</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y044"/>
    <dbReference type="OrthoDB" id="2264294at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001080">
        <property type="term" value="P:nitrogen catabolite activation of transcription from RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050797">
        <property type="entry name" value="Carb_Metab_Trans_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN DAL81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="20"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4364152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="660" mass="74644" checksum="5130B23F3D92080E" modified="2023-11-08" version="1">MSEEPTPRKRRQPAQRSSRPCDSCRRRKTRCIIEAGSQKCGICIQRQSECTFVKEVPKRSFPTPPETNQVSPNIHLSPAAHELLEPEQYPSEVRGTTATSDGIDPHYQAGLHQLESTRTGLEGSLGLNTFKFAELYGLGSDMEPILMRHRPYDATSNEYSLPTHSIRLVSYQDQGVDYPVTFHMVANDKSINYTPDFAEVDAIEACVRPHGEKLIRLFWQMVHPWYPVIYKHGFMLKYAKSYRCIDPPLLGAMYLNAVNWWYYDSHLSNQTVVDVANLHRLTHQSIQNSYHRPRLSSIEAILLCLQCKPEDPLNPDHSFSWGLTAQALSIGEALGLHLDASNWAIPSWEQSLRKRLSWALYMQDIWTALAHGRPTHISEDDWAVTDLTYNDFDTDNPRDAGEHYLHMTTLTKILYTVMKRFYTLKSTTMQNTEELFSMARPILTALESWYTALPSSLNIENLPPRQLCGHGNVHLAYHIVRVTILRRLVRSTALAPLCLDIPVLSFIRQQAHETAQQATSLVASLRLEHLDAFWYFASPYCFSVIGSFCTLLLVTGLTPAERDHWRETLRTYLWTLRVGSKSNEPMRYAVNRLEGAILSGLEHALAVAVDATSPITLEATPGQFQADMNGFGFAFLGADGVDLNTMDLGAFDLLANTGPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0K3</accession>
    <name>A0A9W8Y0K3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MFS transporter (Seo1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SEO1_2</name>
        <name evidence="8" type="ORF">N0V83_009424</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0K3"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF15">
        <property type="entry name" value="TRANSPORTER SEO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="232"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="313"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="377"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="386"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4363970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="410" mass="46481" checksum="96D9D5CBF9078C2A" modified="2023-11-08" version="1">MKLDCIIVPYLFVTYWVKNLDQNNLSESNAPFIENKMEGRAVSTNFFPLENAYVAGMKEELGFYGNELIQLQTMYIIGAVLGQIPFLFLLTYVPMYWLVPGMDVMWGVFTLLQFRANSYAEMAAYRFFIGWFEALGIVATKSVAEEVCAGTIDKPNKLVLSEQDLEIARQRPKRNGHKVQSKLKLRHIKQIMSSPHFWIVVFVDVLFWNAGVNSGAFLLWLKILKKFDTATVNGYGTIPPAVGIFLVLFAKFSSDLLWGSVWGITFATIQTIWKIPEPAKWFTYCNFGWSYALSSVLHGWVNNILRDSHEIRSFTLVFINIIAQSSTAWTGLLAYKTVQAPRFPHRYAFSLSMSTSLIVAAHVLNLYLKKRRDVGHGDARTESDDVSLQEQAYDQSTSKSLPGPKVTVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0L9</accession>
    <name>A0A9W8Y0L9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_009180</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0L9"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032156">
        <property type="term" value="C:septin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000196">
        <property type="entry name" value="Cell division control 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="5">
        <location>
            <begin position="86"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="423"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="423"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4364584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="461" mass="52625" checksum="092623E5D932B3DF" modified="2023-11-08" version="1">MEANGASPAPPTRASPSPPTSSNGYAMDQTLSMANGHSPAPQTVTAKNPKAAAAMANDMNIVRRKLTGYVGFANLPNQWHRKSVRKGFNFNVMVVGESGLGKSTLVNTLFNTSLYPPKERKPPSLDISKTVSIQSISADIEENGVRLRLTVVDTPGFGDFINNDESWDPIVKNIEQRFDAYLDAENKVNRMNIVDNRIHAVVYFIQPTGHSLKPLDIEVMKKLHTKVNLIPVIAKADTVTDDEIDNYKKRILADINYHRIQIFEGPRYELDDEETIAENQEIMAKVPFAVVGSNTEVTTIDGRKVRGRQLPWGVVEVDNEEHCDFVKLRQMLIRTHMEELKENTNNALYENYRSEKLTQMGVQQDSSVFKEVNPAVKQEEERSLHEQKLQKMEMEMKMVFQQKVQEKESKLRQSEEELYARHKEMKDQLDRQRQELEEKKARIESGRPLEEKGKRKGFSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0V2</accession>
    <name>A0A9W8Y0V2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fork-head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_009575</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0V2"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881">
        <property type="entry name" value="CHECKPOINT SUPPRESSOR 1-LIKE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881:SF5">
        <property type="entry name" value="FORK-HEAD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="8">
        <location>
            <begin position="166"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="166"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="250"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="391"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="132"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="256"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="391"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4364120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="440" mass="49587" checksum="586646FA04ED76F6" modified="2023-11-08" version="1">MQWAAPSNFPLDYMGQDLTVQLAPSSEDFYHCAQNGTSYNRNHDFTYPVPYASTSCPRSQSGFDPIGLPNGLPNDFSMSEAYPPAAYQIEPQKPLDTTDLTDQEINGQLMQSSEDYEYRQYGSQTRLEDQRSYQSPYSNLTRDSTPNDEIIRYSHDPNAGEAGAIDKEQPYAQLIYKALLDAPDNTMILRDIYDWFKKYTDKASASETKGWQNSIRHNLSMNGAFEKVDQPCEDSRKGFMWRLTQDAVREGVKSTTRYRSKQPHKRGHHTQHPQPQRQASGAKGGQAARRSARMRRSHRVHEYRSDPYNSRSAPVAFDPMYNTREAPMPYPPSPYTGSEVNYAYGSKPGEFGSPLDAPRFDLFSPPRSYAGSSMLQNMPILDTTCMLDQSPTESLFTDSHSPSPPADEPRTPVTQGSWAEQESLGAPSMFEGLAYRGYAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1M4</accession>
    <name>A0A9W8Y1M4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Cation-transporting ATPase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">SPF1</name>
        <name evidence="18" type="ORF">N0V83_009311</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>[protein]-with a C-terminal TM segment(out) + ATP + H2O = [protein]-with a C-terminal TM segment(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66168"/>
            <dbReference type="Rhea" id="RHEA-COMP:16963"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:90782"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="48943at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015662">
        <property type="term" value="F:P-type ion transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07543">
        <property type="entry name" value="P-type_ATPase_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000049">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000054">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000071">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057255">
        <property type="entry name" value="2TM_P5A-ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047820">
        <property type="entry name" value="P5A-type_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF7">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE HELIX TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23143">
        <property type="entry name" value="2TM_P5A-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="P5A-ATPase transmembrane helical hairpin" evidence="17">
        <location>
            <begin position="48"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="16">
        <location>
            <begin position="288"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1290"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1290"/>
            <end position="1316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF23143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4364714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1322" mass="147266" checksum="2BFCA73A0971BFBC" modified="2023-11-08" version="1">MQGDGDLFLRAILPSLTSKWTRHIHLLSNMAPLVDDPKIQQASLHNPLPLQLHTYIWPFLIIWPAWSALYLSPTRYEKYIQSSEWTFVWVATIVTFQSLFWLMTHWNVNLKSAFTTTSARDVRAAQLIKVHPIKNAGAAEICKLVRDNVGGKQNLSFLFQKRRFLYNAEKGSFAPLSYALDTEPKPQLKTFQQTQGLVSPAEIERLTQHYGDNAFDIPVPTFTELFKEHAVAPFFVFQIFCVGLWMLDEYWYYSLFTLFMLVAFESTVVWQRQRTLNEFRGMSIKPYEILVYRQKKWQEIMSDKLLPGDVVSAGRTKEDSGVACDMLLLEGSAIVNEAMLSGESTPVLKESVQLRPGEARIEPEGVDKNSFLWGGTKVLQVSHGSNAEEDGGNVSRLSSGVPPPPDKGAVAVVVKTGFETNQGSLVRTMIFATERVSANNVEALFFILFLTIFAIAASWYVWQEGVRLDRQRNKLLLDCVLIVTSVVPPELPMELSLAVNTSLAALSKYAIFCTEPFRIPFAGQVDVACFDKTGTLTGEDLVVDGIAGLSLGQNGVKAGPDGAHTDLLKVTDVGTETTLVLATAHALVKLDDGETVGEPMEKATLQSLGWKLGARDTLTAATTTAKSHAELVHIRRRFQFSSALKRQSSVATVLVNNAKTGRKIKSTLAAVKGAPETIRKMLVNVPPNYEETFKHFTRNGGRVLALAYKFLSEDGEWGQNRINDLKREQVESDLHFAGFLVLQCPLKPDAIEAVRMLNESSHRVVMITGDNPLTAVHVAKQVEIVDRDCYILDAPEHDESGEKLVWRSVDDKTNIPVDPTKPLDAEILKSKDICITGYALAKFTDQPGWKQILRHTWVYARVSPRQKEDILLGLKDCGYTTLMAGDGTNDVGALKQAHIGVALLNGTREDLDKIGQHFRNTQMKAVYEKQCSLMQRFNQPQPPVPLLIAHLYPPGPTNPHYEKAMEAQAKKKGLVPEANGVANGSAGTLVKQQPGQVQPAQAAGNFAQQMQEKMMQQEMDELSNEPPTIKLGDASVAAPFTSKLANVVAIPNIIRQGRCTLVATIQMYKILALNCLISAYSLSVLYLDGIKFGDGQVTISGMMMSVCFLSISRAKTVEALSKERPQHNIFNTYIIGSVLGQFAVHIVTLIYVSQYVQRVEPKDPNPDLEKDFEPSLLNSAIYLLQLIQQISTFAINYQGRPFRESIRENRGMYWGLVLVSGVAFSCATEFIPELNTKLKLVPFTSDFKVMITSIMALDFVACWVIEKGLKLAFSDNRPKDIALRRPDQLERERARKVEEEHEAQRKKNAEIEEKARAAGLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1V7</accession>
    <name>A0A9W8Y1V7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10 11">Catalase-peroxidase</fullName>
            <shortName evidence="10">CP</shortName>
            <ecNumber evidence="10 11">1.11.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Peroxidase/catalase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">katG</name>
        <name evidence="14" type="ORF">N0V83_009962</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 10 11">
            <text>2 H2O2 = O2 + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:20309"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="EC" id="1.11.1.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10 11">
            <text>H2O2 + AH2 = A + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:30275"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="EC" id="1.11.1.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="10">Binds 1 heme b (iron(II)-protoporphyrin IX) group per monomer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="10">Formation of the three residue Trp-Tyr-Met cross-link is important for the catalase, but not the peroxidase activity of the enzyme.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the peroxidase family. Peroxidase/catalase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.21" evidence="10 11"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1V7"/>
    <dbReference type="OrthoDB" id="407695at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004096">
        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070301">
        <property type="term" value="P:cellular response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00649">
        <property type="entry name" value="catalase_peroxidase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08200">
        <property type="entry name" value="catalase_peroxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000002">
        <property type="entry name" value="Catalase-peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000004">
        <property type="entry name" value="Catalase-peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000002">
        <property type="entry name" value="Catalase-peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01961">
        <property type="entry name" value="Catal_peroxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000763">
        <property type="entry name" value="Catalase_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00198">
        <property type="entry name" value="cat_per_HPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011635">
        <property type="entry name" value="PRK15061.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30555:SF0">
        <property type="entry name" value="CATALASE-PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30555">
        <property type="entry name" value="HYDROPEROXIDASE I, BIFUNCTIONAL CATALASE-PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00460">
        <property type="entry name" value="BPEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="6 10 11" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="5 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="3 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1 10 11" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="13">
        <location>
            <begin position="126"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="13">
        <location>
            <begin position="476"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="cross-link" description="Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with Trp-92)" evidence="10">
        <location>
            <begin position="240"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4363666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="757" mass="84053" checksum="B6CA3AE0DDF12FD4" modified="2023-11-08" version="1">MSKGECPMKEANVAGGGTRNQDWWPNELKINILRQHDPRQNPLGADFNYAEEFKKLDYNALKKDLTDLMTDSQEWWPADFGHYGGLFIRMAWHSAGTYRVTDGRGGGGDGQQRFAPLNAWPDNVSLDKARRLLWPIKQKYGNKISWADLILLTGNVAIESMGCPTFGFAGGRSDTFQSDESVYWGGETEWLGNEVRYSDGNKGVPGQGVVDGDEHKKEHSDIHSRDLEKPLAAAHMGLIYVNPEGPDGVPDPVAAARDIRTTFSRMAMNDEETAALIIGGHSFGKTHGAAPSENTGPDPNGEDLAAQGFGWINKHGSGKGPDTITSGLEVTWTGTPTKWSNKYLEYLYKYEWELEKSPAGANQYVAKTDDHIIPDAYDQGKKHKPRMLTTDLAMRMDPGFEKITRRWLDHPEELRDTFVRAWFKLLHRDMGPRSRWIGPEIPKEVLLWEDPVGEPEGEVIGESDISNLKKEILNAGIEPAKLIRTAWASAASFRGSDKRGGANGARIRLAPQKDWEVNNPKELAEVLKSLEGIQSKSSKKVSLADLIVLAGTAAVEKAAGVSVPFTPGRTDATQEQTDAQSFEHLEPVTDGFRNYGKGTERVRTEQLDVDKSHLLRLTAPEMVVLTGGMRALNANWDGSSHGVFTKRPGQLSNDFFVNLLDMATEWKPADSGKPSELYEGKDRKSGEKKWTATRHDLIIGAHPELRSIAELYAQPDNADKFVKDFVKAWEKIMNNDRFDIKNKSVSGTSSVSNKPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y487</accession>
    <name>A0A9W8Y487_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribosome-interacting GTPase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RBG2</name>
        <name evidence="5" type="ORF">N0V83_007411</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y487"/>
    <dbReference type="OrthoDB" id="603at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000003">
        <property type="entry name" value="Developmentally-regulated GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000740">
        <property type="entry name" value="Putative GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1070">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="3">
        <location>
            <begin position="65"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="4">
        <location>
            <begin position="292"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4366883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="368" mass="40801" checksum="C8CB54FED66AB13F" modified="2023-11-08" version="1">MVNITEKIKEIEDEMRRTQKNKATEYHLGLLKGKLARLRAQLLEPGPGAGGGGGSGFDVSKSGDARIALVGFPSVGKSTFLSRVTKTKSEAASYSFTTLTAIPGVLEYGGAEIQVLDLPGIIEGAAEGKGRGRQVISAAKTSDLICMILDATKKAEQRRLLEAELEAVGIRLNREPPNIYLKPKKAGGMKINFQAPPKHLDEKMVYNILKDYKMLNCEVLVRDENATVDDFIDTIMKDHRKYIKCLYVYNKIDSIGLPHLDELAREPHTVVMSCELDLGIQDVVDRCWEELRLIRIYTKRKGIDPDFSEALIVRHGSTIEDVCDQVHRTLKETFKHALVWGASARHVPQRVGLGHVVSDEDVVSIVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4F5</accession>
    <name>A0A9W8Y4F5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4F5"/>
    <dbReference type="OrthoDB" id="10009520at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="482" mass="53476" checksum="481D6A8F1EBDCD09" modified="2023-11-08" version="1">MDRFAVYHGNAVVDLSSDSEDGRQADDELEFFDARLAHEPADNLPRPPSPFYDFGVNGHETIDLTAIPDIDVPPSDPVLRGGGAPPLTSQSSKRCGDAQLVTEAACLQMVLGVLPDISVDYVLKVIQERTTDDTRTLAMCEQVVTYLLDVESYPKEADEAKNKKRKREDDDNLSDYEKGERDPEVTGYEHDAVLEDQVRNYQRVASPFSKIGKSRNKRGIELILIERGSRLPKELHAAKKKSETEADDFYVCSKNVSDYQHFGEAQGGKCPLHDSIEARHEQEVKKAADEAMAKVRADNPGLSDADLMIKVSDRVKQAEDARKSQGVADANAFPYRMVGNVLHRAIPVAGVGAQPAVPPPLQFARPAAQYVPPPPYAIAHLPVLVQPVRPVEFGQPFGYIGPAPQQQPQPMPLSLVCYHGKSHYDTLNHLSKIETSIAVYCDRYRRSESSTQRHIIGRSELGRKGLMRRGLRKSIAYARRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y520</accession>
    <name>A0A9W8Y520_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Autophagy-related protein 101</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_007364</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATG101 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y520"/>
    <dbReference type="OrthoDB" id="10259639at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012445">
        <property type="entry name" value="ATG101"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13292">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13292:SF0">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07855">
        <property type="entry name" value="ATG101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4366837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="193" mass="22448" checksum="C8E139FE9D92A690" modified="2023-11-08" version="1">MEPRRRPEYNLDIFADAACVQEVVKAILHTIFFHRYFTSISPLTHDLLDLTLPAIDDVDLETLIEQKTWALVRAIDNTHQPRGRAQIAVQFFEKRRRKTYFSFGKADEDVCWEQWTLDVTLAQPRTETDVLKVRRAMTKSLEKAALKIIAIVNRDKDHIPPITTTDANPFPYQIVVNPKAVSENEWRPRMGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5B8</accession>
    <name>A0A9W8Y5B8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">L-rhamnose-1-dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">DHG2</name>
        <name evidence="5" type="ORF">N0V83_008004</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5B8"/>
    <dbReference type="OrthoDB" id="417891at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019301">
        <property type="term" value="P:rhamnose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000417">
        <property type="entry name" value="Glucose 1-dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF83">
        <property type="entry name" value="(3R)-3-HYDROXYACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0684">Rhamnose metabolism</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4366368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="269" mass="28299" checksum="FC2CCDAC47FF6D2D" modified="2023-11-08" version="1">MATPQLLTGKTMAITGGVTGIGRAIVLGYLSHGANVAVNHFDDPKSCSQYQSLVEEAVETLKTSKEQVEQRLIDVPGDVGNPETGKKLVKAVVDKWGRLDVVISNAGICEFKEFLEITPDLWSSTLTTNLSGAFHTIQAGAQQLSSQTPPGGSIIGISSISALVGGASQGHYTPTKAGVLSLIQSSACALGKYNIRCNALLPGTIKTQLNEKDLEDEEKRKYMEGRVPLGRTGVPADMAGPAVFLGSELSAYVNGAQLLVDGGLFVNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5F3</accession>
    <name>A0A9W8Y5F3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Prefoldin subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_007086</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5F3"/>
    <dbReference type="OrthoDB" id="2015447at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="5"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4367503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13513" checksum="D2F1AB2535FA1FF5" modified="2023-11-08" version="1">MSMPNEALQKVLQEISQKKAFAEQQLIIVRQQKTARTRESRMLQLTSSEVSSLPSETKVYEGVGKMFVCTPIPDVQKRLKDEGEALTKEMSNLDKKEDYLEKTYTNSKNSLEQVLKGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6F3</accession>
    <name>A0A9W8Y6F3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cofilin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Actin-depolymerizing factor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_007461</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the actin-binding proteins ADF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6F3"/>
    <dbReference type="OrthoDB" id="10249245at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11286">
        <property type="entry name" value="ADF_cofilin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017904">
        <property type="entry name" value="ADF/Cofilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11913">
        <property type="entry name" value="COFILIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="6">
        <location>
            <begin position="5"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4366931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="151" mass="17074" checksum="553643FE72C88B71" modified="2023-11-08" version="1">MAQSGVSVAPECIQAFNELKLGKDIKWIIYKISDDWKEIVVEETSKEANFDAFREKLLNAKSKDRRGKEGIGGRYAVFDVEYELESGEGSRSKITFISWTPDDAPQYPRMMYSSSKEAIKRALNGLAADIQANDADDIEWDNIKSRVSKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y780</accession>
    <name>A0A9W8Y780_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF202 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_006956</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y780"/>
    <dbReference type="OrthoDB" id="5525680at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052053">
        <property type="entry name" value="IM_YidH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187:SF3">
        <property type="entry name" value="DUF DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G11150)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187">
        <property type="entry name" value="FGR18P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="DUF202" evidence="6">
        <location>
            <begin position="65"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4367374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="176" mass="19960" checksum="7A4F64B6C2F518A9" modified="2023-11-08" version="1">MDFRSLDRMGESDEAILNEPYRPFYNPFRSKPILVKQDNEYDRTVFGERPLLGVLLFDNVSSDARDHAANERTFLSWLKLSVYMAIVSVAIVLSFHLKSKPSALEKRFAVPLGIIFWILSLACLLSGLSNYINTVTRYSQRQALVQSGWKTQVVFTVVSTSIVATCVLFLSTNAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7E6</accession>
    <name>A0A9W8Y7E6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RNA recognition motif-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NOP4</name>
        <name evidence="8" type="ORF">N0V83_006703</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7E6"/>
    <dbReference type="OrthoDB" id="267048at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12676">
        <property type="entry name" value="RRM3_Nop4p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12677">
        <property type="entry name" value="RRM4_Nop4p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000406">
        <property type="entry name" value="Related to Nucleolar protein NOP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034809">
        <property type="entry name" value="Nop4_RRM4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034808">
        <property type="entry name" value="Nop4p_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051945">
        <property type="entry name" value="RRM_MRD1_RNA_proc_ribogen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48039">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN 14B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48039:SF5">
        <property type="entry name" value="RNA-BINDING PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="41"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="149"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="312"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="496"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="234"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="651"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="239"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="263"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="652"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="695"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="709"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="728"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="750"/>
            <end position="766"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="766" mass="85590" checksum="8C9835BCC2D38419" modified="2023-11-08" version="1">MAPRRKRQRLSLDGAEAVTAVADAEQPSVSTSTAPKGAGRRQLFVRGLAPDVTTQDLTDYFSESYPIKTAVVVLDKESRESKGYGFVTFADVEDAQRAKEELNGTELKGKKLRIDTAEARQREGEKDTVKASDRIKAEREQQIKEAQTPKLIVRNLPWTIKTPEDLQKLFRSYGKVNFVNLPKKPNGELRGFGFVSLRGRKNAEKAIQELNGKEIDERPIAVDWAVDKETWQGLQKTEEVDEASQAEATEDDNLDAESSAVSSDDDSQVDDSEDDEDLDDSNTDYEDVSDDDDAEGGVELEEEDDKPKREEYTVFIRNLPFTVDDERLMEHFKQFGGVRFARVVVDRETERPKGTGFVSFFAEEDMINCLKGVPRVKLQKRNLDKKDGSTITVTHSVLEDDDADPTGRYTIDGRILQITRAVDKNEATRLTAEGAATRFNRDKDKRRLYLLSEGTIASNSPLYQQLSPSEIKMREESATMRRKQIQENPSLHLSLTRLSIRNIPRSITSKDLKQLARSAIVGFAADVKAGKRQKLNKEEVIRGGQEMLVAEKMRKKKGKGIVKQAKIVFETPAGSKVAEDTGAGRSRGYGFIEYYTHRSALMGLRWLNGHAVDYKIKGETPKSKKKAKEALEDKRKRLIVEFAIENANVVSRRSDREDKAREHPKPKGGEDNAQAANRDSKAGATRGQKRKRDSSTTSKAASDAAQQKPGHKGSRKRSQVQGSSGAEEDGRAGTGGKDKDQDKLAQRTRIIAKKRQARKAKRAGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7K5</accession>
    <name>A0A9W8Y7K5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein BTN</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">BTN1</name>
        <name evidence="10" type="ORF">N0V83_006260</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Vacuole membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the battenin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7K5"/>
    <dbReference type="OrthoDB" id="5965864at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003492">
        <property type="entry name" value="Battenin_disease_Cln3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018460">
        <property type="entry name" value="Battenin_disease_Cln3_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981:SF0">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02487">
        <property type="entry name" value="CLN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015974">
        <property type="entry name" value="CLN3_BTN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01315">
        <property type="entry name" value="BATTENIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="213"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="230"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4369176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="405" mass="44194" checksum="E4CDE6CFB5109D50" modified="2023-11-08" version="1">MPGSPASSWAVYRARLKAVFHGADPRVCAAFWLFGLINNVLYVIILSSALDLVGPNVPKGVVLLADVIPSFLTKLCAPYFIHKIPYNVRILIFVALSACGMLIIALTPQLQDSRTIAIKMCGVMLASLSSGGGELSFLGLTHYYGHFALAAWGSGTGGAGLIGAGAYAIATNTLQISPRTSLLAFSFLPLIMVFSFFVILPLGPLRAGAERHGEYEPVGSGERDEEVDEETSHEREHEGLLSSSSTSGKSVTGAMNSFRANLHRARGLFFPYMLPLLLVYIAEYTINQGVAPTLLFPLSSSPFTEYRSFYSTYNAIYQVGVFISRSSTPFIRIHHLYIPSFLQVANLLLLTLHAMFDFIPSYYIVCVVIFWEGLLGGLVYVSTFAEITDNVPKEDREFSLGATSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7V5</accession>
    <name>A0A9W8Y7V5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Quinate dehydrogenase</fullName>
            <ecNumber evidence="2">1.1.1.24</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">QA3</name>
        <name evidence="2" type="ORF">N0V83_006361</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.24" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7V5"/>
    <dbReference type="OrthoDB" id="204377at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030266">
        <property type="term" value="F:quinate 3-dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019632">
        <property type="term" value="P:shikimate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01065">
        <property type="entry name" value="NAD_bind_Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022893">
        <property type="entry name" value="Shikimate_DH_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089:SF1">
        <property type="entry name" value="BIFUNCTIONAL 3-DEHYDROQUINATE DEHYDRATASE_SHIKIMATE DEHYDROGENASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089">
        <property type="entry name" value="SHIKIMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shikimate dehydrogenase substrate binding N-terminal" evidence="1">
        <location>
            <begin position="19"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="309" mass="34323" checksum="8026ACAF2B8BCA40" modified="2023-11-08" version="1">MTTETVTSEISQVERHGYLFGHPISHSMSPLLHKTVYDNLGLNWAQYPMDSIDMNLFLQRIQHPQFYGASVTMPHKVAIIKHLDELTEEGAAVGAVNTIFIEEDSTGKRLFKGTNTDVIGVRDAFCYSVAPEKYYNRPALVIGGGGAARSAVYALRTWMKASAIYLVNRDPEEVRAVINECTARGYGDSLVHVSSVSQAEQLEGVGAIVACIPNFTPKSDAEKEARKILETFLQKSHKGAILEMCYHPTPWTEITELSEKAGWQVVLGTEALIYQGLEQDRYWTGKEVHQLPVEKVQQAIAAKLSEPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y870</accession>
    <name>A0A9W8Y870_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gelsolin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y870"/>
    <dbReference type="OrthoDB" id="6375767at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008154">
        <property type="term" value="P:actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11290">
        <property type="entry name" value="gelsolin_S1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF130">
        <property type="entry name" value="SEVERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00597">
        <property type="entry name" value="GELSOLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gelsolin-like" evidence="1">
        <location>
            <begin position="63"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="1">
        <location>
            <begin position="195"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="1">
        <location>
            <begin position="321"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="391" mass="43864" checksum="78F452D34FF9171A" modified="2023-11-08" version="1">MPPHAGLVHLEGYDWRDSNIALVNSDIDHKVKYQSATTEPAWNDGIIGVRPGVFIWRIEDFEVVPWPSKRYGSFHEGDSYIVLKSESIKADGHEDKLIHDIFFWLGRHTTQDEAGTAAYKTVELDEFLRGTATQHRELQLSPSDAFLQLFPRVKILRGGVQSGFTHINLDEEVQHVNALLRVFKHPGAAASRDAILVHEVEPSWKSLDEEDVFIFDKGDKVFVWQGKRCSPMEKLKAAQVVNDLTIAKHVDVEVLSQTEARSKIIVDALGGQEIDSFSTEFHCARPVSTATEREVKKLFRLSDAEGDLSFDLVKEGNGIEKGDLDADDVFLLDAGKSIWVWEGQGASTVEKAMWLKVVQRYVSQQQNTADLSVGKVRQGHEGQAFWTAVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8G9</accession>
    <name>A0A9W8Y8G9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_005880</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8G9"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000007">
        <property type="entry name" value="NAD(P)H-dependent D-xylose reductase xyl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="6">
        <location>
            <begin position="39"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="5">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4368798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="337" mass="37279" checksum="D2E71E098E1980A5" modified="2023-11-08" version="1">MNPSLANFMLKSTPDKIIHPEPTMNTAIPLNTGATIPALGLGTWQSAPGEVKKAVTHAIEAGYRHIDCAFCYQNEDEVGEALQDVISRGIVKREDLFITSKLWCTFHTRAEEGLQKSLDLLKTPYVDLYLMHWPVPMNPKGNHPLFPKLEDGSRDIDRSITHVDTWKNLEKLIQSHPDKVKAIGVANYSVKYLEKLLAKATITPAANQIENHPLLPQQEVVDFCKEKGIHITAYSPLGSTGSPLFKDEGVNEVAKKHSVGPATVLLSYHLARNSSVLAKSVTPSRIDENRKLISLDAEDMEKLESIHKTKGITRYVYPPFGVNAGFPDKPDGIDLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y919</accession>
    <name>A0A9W8Y919_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_004358</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y919"/>
    <dbReference type="OrthoDB" id="3774349at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052783">
        <property type="entry name" value="Metabolic/Drug-Res_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47655">
        <property type="entry name" value="QUINIC ACID UTILIZATION ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47655:SF2">
        <property type="entry name" value="QUINIC ACID UTILIZATION ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="12"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4371142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="699" mass="76799" checksum="28AD8DC9F15D5571" modified="2023-11-08" version="1">MTPSVRARVSRACNECRKRKDRCNGARPTCDNCVRTDRACSYWPSKKRGLRTGYVRALETLLGLLLSSIHGADPWLSSLLGGHETQPPFQLQTPAGDTTDALLDAWRAARVSKRIDQLLAPVDDEDGAESNSLFDRRVSHASATVAKALSNPLPMDIDASPMLADGPADEPLAEPFDEPVQLPASPITASQPARLAAEPSLCYTLPTNWQYLLNYHFAATHCWLPMSQKHHLLRSAYSVHNSAGPDPISPGDWTFLTAVLAYASFQSESQGSGMQPDQSQVLSLSSTIMGLLPNDSNFQPGHIRALLILTLVQIYDQNEKAAWSMVGRAAYAITCLEDSPFKEPKRSTILDDDTKRTILCCIVLEAIVASWVRARPYFQRSDLALVGNLQADGMEEWEPWQPIEALNNGILSIQTPGHVLSIFNHVTDLAGLLIDLLRLPCDGSRTSSVSNLYQRFREWKQCLSAQHPTMLTTSTHPHLLNLQLFSASICEAIKTEEMLSTGVLSSSSHAETCTHLREHGKLLHEYIQNMGPRLVPPTSNVYLSILCLSLNSRAQLQLGDLAALEDIQGLRQWLLELNNTRVYLGQALSTNILPNLPETALSLQDQVTEVHHLDSGLDMASSSTWNAPAQPEPSLSTALLPFASQEQPGVPEDVVFDPMLALETGEMSEDGLFNSLADLDSADWYARDSSRALLVTNAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y946</accession>
    <name>A0A9W8Y946_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Trehalose-6-P synthase/phosphatase complex subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TPS3</name>
        <name evidence="7" type="ORF">N0V83_005206</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y946"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005946">
        <property type="term" value="C:alpha,alpha-trehalose-phosphate synthase complex (UDP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000099">
        <property type="entry name" value="Alpha,alpha-trehalose phosphate synthase subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1020:FF:000001">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000036">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase subunit Tps2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1020">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase related protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF15">
        <property type="entry name" value="TREHALOSE SYNTHASE COMPLEX REGULATORY SUBUNIT TPS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="29"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4370685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="922" mass="104223" checksum="A6E8C74BFFC3C3FC" modified="2023-11-08" version="1">MLSVRPLREAGTFRFLPYTVDFLHDNKIESKSNQTSPARPAPPKLAEVHRKPSVSSSIHSKHEEQPVSLLRRAPPSHIQLPKAPLRMLELEEFFTPGQPSAATHFPKPTDPRGLVRSDAHVPEWGASGLFFNQPQSRADPAPPDTILEYAKAQERAQAQKERMKGSQAGKLTTDPRWATDYKVVPAVQGNGGLTNALRCAVNNKCIKDVLTVGLIGFPTDSLDEDKKAEIYEKLEEEHEAFTVFVSDKDFDGHYAHYCKTILWPVFHYIIPDHPKSKAFLDHSWVYYVKVNQAFADKVVKNYKRGDIIWIHDYHLCLVPQMIRQKLADAQIGFFLHTAFPSSEVFRCLAARKELLDGMLGANLVAFQTNEYAHHFLQTCSRILSVEATEDGVQLENRFVNVWSSPIGIDPHELGIAREVPEVLEWIQTMQDRYKGKKVIVARDKLDSIRGVRQKLLAFELFLNKNPEWRDKVVLIQVATSTTEDTELAATVSEIVTRIDAVHSTLTHNPLVFLRQDIAFSQYLALLSIADALAITSLREGMNLTCHEFVICQDGKASSKKHSPVILSEFTGSAAIFDGADLEVNPWDYNNIAEAFKIALEMTDEEKETRYAKLRNIVMHQTGDVWVSNLSGHLAKVHEEQFKRDTMSIPRLSYNNLSRAYENSWKRLFILDYEGTLAAFGSVSNTVLTNTERVVDILNDLVADEKNAVYVMSGLTVNETELVFDRVRGLGLIAENGCFLREGNSDEWTQFPDEEKTVKWKESVKPILQYYVERVEGSWVEERHCSLIFHYERTYDNETSSRHAGDCANHINDACEQQRVKAVPTKDSVVIEPVDFDKATAAQHIFDKYPETARPDFLLVAGNDRSDENVFRWANQLKDKWVVPNVQTVTVGDRNSVATSTLTNGTTGLLSALSKLTKIRTTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9U7</accession>
    <name>A0A9W8Y9U7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6536 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004796</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9U7"/>
    <dbReference type="OrthoDB" id="5429634at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046623">
        <property type="entry name" value="DUF6536"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35395">
        <property type="entry name" value="DUF6536 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35395:SF1">
        <property type="entry name" value="DUF6536 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20163">
        <property type="entry name" value="DUF6536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="376"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="464"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="519"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="578"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="629"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6536" evidence="2">
        <location>
            <begin position="86"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="733" mass="81249" checksum="D940BF50E0A50473" modified="2023-11-08" version="1">MAEQNVILSTYSSRDKDDSKSDTKIGVTSYVRPANSDTAYAAGSFSTNEYKVSHSKTLVERFEEEEHRLFGTRVFGFFYDTFVAGWRAGLLRAFLFSLIALIVNVAIYAWLFSTYKASEGTATVMTGDCRRISKANTGIHLALNVVSTLVLGASTYAMQGITAPTRKEVDAAHAKGNFNAVFFTTSQANQYAVAVVEESYFQNTYLRPSAENASPDLFEQWAYEKQDCDDGVCSFDVRNISTVVELLQDVRSLTRNAKFVQMSPQECMQNYSTGFLKDHSDVVVVSSRSDANSSILWTRYPQRSVTADKSHTNNDPFHWICHDSLDSGNTEDDHCDLQLAMDHFDSGKNWTVYGNPVDHCFARISPDVCQLQFNEWLMLVVVVCGAIKVLTMCYLVFWRPAGRFLRTLGDAIASFLEEEDATTKDMCLVSSKQVRKHGFRTPYDPQTYTGIRPRWLSGANTIEFFATIGISALYILVLAIALSFAIDGADGFAFSNGLGVPDIQSLVSFKRDDTGSSGIVPTLIIANIPQLGFSLLYVVYTSIWGKLLVAQEFDRLTQTKKGLRVSERPRGMQRRSRLLTLPMRFALPLMVCSAALHWLCSQSFFMVRIDGVNAHREIDPNDRLVRLGYSATGVVSLIAVSVAMLLATVCVGAFRRLRTGLGETSMSVVISAACHVMRYEAEPWLQEVQWGDVGAATTATAEGAQSLEGLDGVRHCAFTARLAERPIVGQAYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA30</accession>
    <name>A0A9W8YA30_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">serine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Seryl-tRNA synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Seryl-tRNA(Ser) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">DIA4</name>
        <name evidence="12" type="ORF">N0V83_004806</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA30"/>
    <dbReference type="OrthoDB" id="10264585at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000069">
        <property type="entry name" value="Seryl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="11">
        <location>
            <begin position="194"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="125"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="326"/>
            <end position="328"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="342"/>
            <end position="345"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="429"/>
            <end position="432"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="8">
        <location>
            <position position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4371587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="506" mass="56670" checksum="326CD9ABDD38AB10" modified="2023-11-08" version="1">MRAIRRSFLDYSRHRCQYASPHRTTLPIACSARRAYTRPAFAPKPTIDIKHIRQNPGLYEQNCIDRNYAAHSKSSWRIIELHEEWLALQQSSRSLRERNNRLRAHLSTAATRMKDADKSKAVPVREGMIAEAKALKTELSAIEARETQIQDEIEGLAQDLPNLSSTHTPVGEEPDVLGYINEHAKSFNTQARKSHVDIGQELGILDFSGSAQTSGWGWYFLVGDGAMLEQALVQYALSVARRKEWKAVAPPSLVYSHIASACGFQPRDQNDEQQIYAIEQSEKDKAKPQLALAGTAEIPLAGMKANQTLEDSELPLKTVGVSRCYRAEAGARGADTRGLYRVHEFTKVEMFAWTAPDSVGEDHFTSPAASSSETIFEELLSIQTEILEALGLRCRILEMPTTDLGASATRKIDIEAFFPSRQKDDGWGEVTSTSMCTDYQSRRLQTRMRVKNGKLEYPHTLNGTALAVPRVIAAILENNWNEQERTVTIPEVLRPFMGGQDKIQVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA73</accession>
    <name>A0A9W8YA73_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_003835</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA73"/>
    <dbReference type="OrthoDB" id="2441642at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF51">
        <property type="entry name" value="QUINIDINE RESISTANCE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01036">
        <property type="entry name" value="TCRTETB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="368"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4372062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="417" mass="46043" checksum="5BE91B487FB15568" modified="2023-11-08" version="1">MSDNSGRRLSFIVCYVIFIIGNIGLALQTNYVALLILRMVQAVGCTAAIALTYAVVADLATSAERGKYMGYAGAGILMGPAFGPTIGGLLAQYLGWRSVFWFLAIFSGVLLVLFIFLFPETCRKVVGNGSIPPTGVNRSVISCMQQRKLAKQTPSGEDMEQTKPEKRKIPFPNPLATFRIIAEKESGIILLYNGFFFTGMMTTVSAIPVLFHEAYELNELHIGLCYIANGMGSLISSLTMGHVVDWNFRRHAKAMGMIINKGKQQDLSNFPIERVRLQIVVPGHILGIFGLLMFGWTVKFQTHIAGPEVALFVIGLGVSTAFNITNGLLIDLHRDQPAAATAAVNFVRCLMSAAGSAAIIPMCHAMNPGWAFTFLALVYVVLIGVIFWVMRDGMRWRQEFEEKKRIKREEQERTEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAI8</accession>
    <name>A0A9W8YAI8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminoglycoside phosphotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAI8"/>
    <dbReference type="OrthoDB" id="191037at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05154">
        <property type="entry name" value="ACAD10_11_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052898">
        <property type="entry name" value="ACAD10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041726">
        <property type="entry name" value="ACAD10_11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47829:SF1">
        <property type="entry name" value="HAD FAMILY PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47829">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12880)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="1">
        <location>
            <begin position="37"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="385" mass="43142" checksum="903613DB2ED0F11A" modified="2023-11-08" version="1">MSGVTKQKTRHDLDDDNLGKYLQQQIPDLKLPVLSTKIGYGQSNPTYFVDDAGNTRYILRKKPSGTIISPVAHQVDREYKVIKALGSVEGFPVPKVYCLCMDTSIIGTAFYVMEFVKGRIITDPNLSTLSPTERRSAWFSLVSTLAWLHSIDPDSIGLSDFGKKTGFYTRHCNTFSRIEAQQAKVKDVTTSKELGRAHPNFDEIVDYIRKNVPSDRTSIVHGDYKFDNLVLHPTEPRIIAILDWELSTIGHPLMDAVYAVGPYWNRASSSGISGGPKAQARDDQGGEVYDPKNQAANGMPSMDELLDEYTKITSWDPRKDQWEIAKVFHLMRGSTISHGIQARTISGQASSEFSHIYFENTKRGLNAALDMVRSMKEKEPARSMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAN0</accession>
    <name>A0A9W8YAN0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Telomere binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TEL2</name>
        <name evidence="4" type="ORF">N0V83_003953</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TEL2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAN0"/>
    <dbReference type="OrthoDB" id="10258062at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.720:FF:000004">
        <property type="entry name" value="WGS project CABT00000000 data, contig 2.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.720:FF:000007">
        <property type="entry name" value="WGS project CABT00000000 data, contig 2.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.720">
        <property type="entry name" value="Telomere length regulation protein 2, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038528">
        <property type="entry name" value="TEL2_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051970">
        <property type="entry name" value="TEL2_Regulation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019337">
        <property type="entry name" value="Telomere_length_regulation_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15830">
        <property type="entry name" value="TELOMERE LENGTH REGULATION PROTEIN TEL2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15830:SF10">
        <property type="entry name" value="TELOMERE LENGTH REGULATION PROTEIN TEL2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10193">
        <property type="entry name" value="Telomere_reg-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Telomere length regulation protein conserved" evidence="3">
        <location>
            <begin position="582"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="514"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="545"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="563"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4372180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="949" mass="105022" checksum="5586A44CC4C19BF6" modified="2023-11-08" version="1">MTDFLTAVSTKKTKDVEPLTQEVKGLDLQGDVPINSAESAVKALKNQPGRGTVNNVLEYLTTGGFNLLLPEPLNASIAHQLVTDTIPNYWRLLKNSTQGKLLAQVLRNPTGIGHTTTRLRTLIADSRHKKSPGEARNTLEHIDDLLDVFDLILHDDHTSKLVLRDVLAYSKNAVQQKLIWREYLAQIASGRILSIAAEAEDVLKKSDISKHGSWIADGNAFAAWIGRNIAVLMVANDEGEERRSAVVELLSKALGLGYTERIISSLISPLIDGDCIVTLTDLLSRMKSFEQRKYFSAVITAVTKHHLASGIATKNDVSMLSTKTISGAASLFYNIITENDILKEQLVSSLTRSTIPSLDDSLAARRSVMASLAKDENALQTLLENSIKLFGDSMYMKHTPVLQQEVLAQNLALCCGYVHRSQPMFLTMMAKSTYHVNGMSNRIGTPSARARFLGIAVGIAISKLIDKPDLRLKFELEDDEAEEANWYQRLTEVDDKVGSIKDLQIQAMSIAPNKSTHSKIKSASRPSKVPAVTEVQGPQIVEVLSESDDEDDDLVPYEKPDSDPEDDGDDPETINRNKPSAPVYIRDLIAALRDQENYDRHELGLATAASLIRRKANFGTEVTDHLEELATILTGLQDNLELESFAQQKQQALIAVLLAKPAQMAQWFAQSFFSGDYSLTQRIAMLTTLGLGARELAGVKDSATEELIPAQPSFPSKQLPPHLHKLYAEDKVGTPVAKISSSMAREMLSPIASQAADQLSGPNILKMRTFSSRMEVEKKRQKPIPNALAQIVADNFFFPLTGRWWLQIRASSDSIYTSTHLLPPFLQTLSLLLNASGPNTLALPQMTREYWDLLLSVRGLASKDKNILNAVLFGFLMILETNENKERLATEQGKELMETQAWVKTIFEGLGAGREEDERIRVLAAGVVVRCQEVVEKYQRRMVGALMDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB32</accession>
    <name>A0A9W8YB32_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mitotic spindle checkpoint component mad2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MAD2</name>
        <name evidence="9" type="ORF">N0V83_003241</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB32"/>
    <dbReference type="OrthoDB" id="1806at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033597">
        <property type="term" value="C:mitotic checkpoint complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.900.10:FF:000002">
        <property type="entry name" value="Mitotic spindle assembly checkpoint protein MAD2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003511">
        <property type="entry name" value="HORMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045091">
        <property type="entry name" value="Mad2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842:SF11">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02301">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="The spindle assembly checkpoint protein mad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50815">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HORMA" evidence="8">
        <location>
            <begin position="26"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="128"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4372950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="232" mass="26089" checksum="B3D7099917F09779" modified="2023-11-08" version="1">MSQPKTSSRSKASKTDSSKVHKLALKGSSKVVNEFFEYSINTILFQRGVYPAEDFTAVKKYGLTMMVTADDQVKAYIKKIMSQLKEWMYGGKISKLVVVITSKETGEHVERWQFDVQIFGRSSKSKTSKNPTDQENSAPESNETPEEKTEAEIQAEIQSIFRQITASVTFLPMLDGNCTFNVLVYADADSDVPLEWGDSDAKEIKNGEKVQLRSFSTSNHRVDTLVSYRLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB71</accession>
    <name>A0A9W8YB71_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">OPT super</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">OPT8</name>
        <name evidence="9" type="ORF">N0V83_004729</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB71"/>
    <dbReference type="OrthoDB" id="627262at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF0">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="332"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="369"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="470"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="496"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="589"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="648"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="209"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="219"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="239"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4371511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="670" mass="72510" checksum="617C7290382364BF" modified="2023-11-08" version="1">MAPDRGSSSQSPRDTERVGEEYDGGRREDETLNQTRSGERRFLADQASQPQNFTLRGVLVGLAIGVVICFSNMYFGLQTGWVSGMAMPSALIGFAYFKVVARTLKLPFTPVENVLVQSVAGSVGTMPLGCGFVGVIPALNYLLTPEENGPLDISLWRLIVWSVGICFFGVVFAVPLRKEMIIREKLKFPSGTATALLIRVLHGDEKSKMMVARERRENEGEESEGEEEAQGLLQGSSVQREEHDLRPSVDDDVREQDREQELGDDGDWKSKIRLLLISFAGSALYTVVSYFIPHLHDIPIFGLPLARTWLWTLNPSPAYVGQGIIMGPATTLHMLIGAIVGWGVLSPLAKHKGWAPGPVSDWTNGSKGWIVWISLAIMLADSLVSLGWLILRPLISISRAYYPTVQSTFQSHTWKELFTLNITSKQRYTPLASSSSDAPSDPLTAIKQSLTSSPTAEPDAPPEHLISHRVTLIGMILSLVLCILAVHLSFPSLIPFRLTLLALLLSLFLSIMGVRALGETDLNPVSGISKLTQLVFALVTPTSGPGAKNAVIINLVAGAISESAALQAGDLLQDLKCGHLLGAAPKAQFWGQMIGSAVGAVLSAGVYKLYTKVYTIPGGLFEVPTGYVWVFTARLVTGKGLPPMVLEWASGAAVIFAVGTVVRVWGIIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC38</accession>
    <name>A0A9W8YC38_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_003576</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC38"/>
    <dbReference type="OrthoDB" id="5571888at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14810">
        <property type="entry name" value="bZIP_u1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000031">
        <property type="entry name" value="BZIP transcription factor (MeaB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37616:SF2">
        <property type="entry name" value="BZIP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37616">
        <property type="entry name" value="BZIP TRANSCRIPTION FACTOR 60-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="3">
        <location>
            <begin position="293"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="39"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="204"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="271"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="505"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="283"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="505"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="517"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="535"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4373282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="565" mass="62448" checksum="EBFFA5BFE612B1E4" modified="2023-11-08" version="1">MATAVQSAMTMAHFNPHQNQCIDPEQEFFDFGALPSPTPATLKRESSATSTITSPTSTAIDEDLQTPAKPSHEYDRFKQQTGLPSGSIAGLSAGYNSGFPMFSSTGLDEIALMHGGSMDGGWNTGMHMDAGMNMGMDYQQSSYMFNDASQDDFVDPSAISQEEVPNVRVWPGMHQQQAALAKAQAQQQRQAQQQLAQQKQQQAMQAQQQQQQHNRLQSTSQANRKTSSPLSDARTEETIARVVAQIRADSQNASSSMQDGNQGLLPHIIRAKKDEDDMDEDERLLASEEGKKLSSKERRQLRNKVSARAFRSRRKEYIGQLEGEVAMKVNEANELRNQNRLLMEENARSRAFIERLLRHQAFGPFLEELSRDEALQPKAPMPATSTPAVAAPAPASFQNQQFSGMSESENLHVGMTMVPETQIDFSMLNLNNNGNNWGSNNGFNFQQPRVFAVLEPTPLDTEAMSGKGYSAIFNAEDDSPVDEVKPDFPVIEHPVQSEKCATVVEEEVEEDSEYDLYNSSPARAPSTATVSAPPREPRAPFHQPREGSRTFLFDHLGRGTGDTIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC67</accession>
    <name>A0A9W8YC67_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bud neck involved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">BNI4_2</name>
        <name evidence="2" type="ORF">N0V83_003636</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC67"/>
    <dbReference type="OrthoDB" id="5563016at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="84" mass="9290" checksum="D608EA61134AF056" modified="2023-11-08" version="1">MASSDMPPPVRPGHNRASSVDDTQIPHSGGIDAAKRYRRRSFNGLEENNLTSNMASLQSGIEYPAKRYEARFSSIEPTIIESQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCE6</accession>
    <name>A0A9W8YCE6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002548</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCE6"/>
    <dbReference type="OrthoDB" id="2590867at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39606:SF1">
        <property type="entry name" value="CELL SURFACE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39606">
        <property type="entry name" value="SURFACE PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="402" mass="39874" checksum="623D4C6BBFB3A74E" modified="2023-11-08" version="1">MAGTMDKIKAKVDQVMHKDKTHDDTTGPHSSHAANEADSRVNTTGPGHTGATGHSTTGTTGGLTGSSTTGGYGNTAGHHNDPTGPHDSKLGNKADPRVDSDRYGAAGNTSGAGGYGTGQYGEGHHTQGHHTAGHGTTATSAGGLTGALGGEHYTSDPTGPHETRTANKLDPRVDSDRYGATGNTSGAGGYGTGQYGEGHHTQGHSTTGGGLTGGGMTGSSTTGGYGHSDPTGPHDSRLGNKTDPRVDSDRYGTSGNTTGAGGFGTGQHGEGHHTAGGAGGFSNTSGGVGHHNDPSGPHDSRLGNKTDPRVDSDRYGAAGNTSGAGGYGTGQYGEGQGHHTQGHQHGHENTAGGYGGGNNTVGSGNSKTTAGPHNSDMLNKLDPRVDSDLDGGKTYGGNATRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCK4</accession>
    <name>A0A9W8YCK4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">1-phosphatidylinositol-3-phosphate 5-kinase</fullName>
            <ecNumber evidence="1">2.7.1.150</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">MDM12_2</name>
        <name evidence="14" type="ORF">N0V83_002700</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.150" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="158357at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000285">
        <property type="term" value="F:1-phosphatidylinositol-3-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03334">
        <property type="entry name" value="Fab1_TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17300">
        <property type="entry name" value="PIPKc_PIKfyve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.810.10:FF:000001">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase FAB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000007">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000283">
        <property type="entry name" value="1-phosphatidylinositol-3-phosphate 5-kinase (Fab1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044769">
        <property type="entry name" value="PIKfyve_PIPKc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748:SF7">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="12">
        <location>
            <begin position="402"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="PIPK" evidence="13">
        <location>
            <begin position="2001"/>
            <end position="2323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="65"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="464"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="502"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="649"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1526"/>
            <end position="1549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1575"/>
            <end position="1619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1704"/>
            <end position="1734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1844"/>
            <end position="1916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="115"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="179"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="237"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="281"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="291"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="341"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="466"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="537"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="553"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1534"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1596"/>
            <end position="1609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1870"/>
            <end position="1883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4373961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="2370" mass="264567" checksum="6903A4C7D7071EE6" modified="2023-11-08" version="1">MARDSRASLHEALHQMHATASRSDTLTTFDDLTAPPAPSGAGEPKGIDLVQGGLSGLYSRLRASVGAAKEQAPEGPSGLAGEARTSPVTKPGAINKSAGGTAVSSPVVVSAPSSRLQSPSTATFPNALPSSRDSNMSNTPMSSNLSLNTSKASLATTRSRPSIAPSIETGKAYNEHEASVQSPVSTHSRAQSASNFEQPRNASVRNFASGARTTGSPRFTPANDDVGRGRKGNLDLEGQDSDSTDENEVEVVAGEGHASNSASFKFPSDAPHESLRRVNSRAESQSQTLNKAREQAEKAPEGTQPQHVATNARVPVLAPEPQRPPLLKVGPSHLPGFRPSRASSSDGLSSVVTASTVRTPAVPPIEQIQRQAQDRPAVETMPKNALTQMRRKILDREFWMRDENAKDCFNCGDAFTTFRRKHHCRTCGQIFDSKCTSLVSGKIFGQPSNLKVCKPCEAIIYGHDDDSSDYTDEGDQASVYDHGEPGLSLDEDELEAPESDYTKIGTPTISIPMSRKTGSEKKHRSHVIEVGPQTLARPSSSRSLRSLSGRPRSSSHKRYHSRHQHMRPVKHDHRAPFHQYHDSSRGQPSLSAFHHDNIIDPDLAHYMSDEDSSEEEHPSIFATLSEDPSANSNLEAEKSGLGGFLAAMRKGKSRSGDKSVASGGHARDTDNISITSRNTNKTRRRNLSVSSIAHRPSPRRSKSNTLLRPFTAGYGASGSISQSGTPLHPSPSPTPLGTRITRSASMQGGITPKVELNKASLDHARKLVRQMLHDAEVPHVSSWEKALIPILRQCTDDVSPDVDRGDDIDVRNYIKLKKIPGGNPRDTAYVSGVVFTKNVALRSMPRSIPNPRIVVITFAIEYARHQTHFLSLEPVIAQEKEYLRNLVSRIAALQPHVLLVQRNVSGLALQFLEQEGITVVYNVKPSVLNAVARCTGLRMISSVDKLAIDPSNLGHCESFDVKTYVHKHFRKTYIFLSGCQKDLGCTIVLRGAEKQELIKLKQITEFMTYVVYNLRLETCLMRDEFIDTPTTSIIGTLASNKSEDDKSKQQHAGNSNATAMAGVEEDHLGDHNDPGDAAPSYYSDLVEKHRTKILSSSPFVKFMQPYLLEQARQYERKLGHLKRLKNQYTVGDEDASDEFGQKFELVQPKMVHTVVERASKQVREFLSAVHASEYDKAMHHYMTQKRQWEQYLSGNKDLFDPFNHQKIAVLYSIVNTGKGTPCIGPEIIALGFYKEHDFDDGFTPDCTLGQYVEHLCTTALDLCEDEGCKRRMLDHSRQYVHGEGQMSVITQKHPAKLKGMYQTILMWSCCRICGQETQAFPMSEWSWRYSFAKYLELTFWSTGLHPRAGICPHDIHKNHVRYFGYNNVALRIQYDPVNLYEVIVPKASVTWNVDSDLRLKNAQYLMIEEKLDCFMDSIRNRIQSIHVDNIIGEKFDLCKLEVERLMNRANEEHEWLKSKLQAKYMESKYYEIIPMNRAIRAIQEKAIAWDETFQDFEQQFFPSEKDIRRLAAVQLRKILFERDESTGSLTSVDESTESGAEDSYVDEKSDSIVLAPKPSDMSPEMAHNVLSSVVKEDQAEGNQSNVATVTAERKTPSPSKDSSELSVQTPREALEREDVRHLDLAISSNFPGPQSTDEQLLKTPTLAPNSDALDEGSPTPISLLKVDPIDKSVADAIENMPSSPTPLPVADTPLESKIPRLVDNNRRETSARPSALTRTQSQPGGIPKHSIPFPGPTVFNSGPILTRQPTLSDHAKALERMTERLGMGPMKQSKTTSRIPRSIPFKPTKVSKLAQHFEQLSREFEKERLRERRNNRNRQARAYPLASSKPVVEVYKDVHDAVQEHNASDEDMQIDSPPRASMDNSTLGDSAITETTGGITTASQSPVEERHPKHVHAQPNESDEPPKTPSHLHSDAELDTSDTEIMPEDIALPDSIASSQLLNLSESQLDSSLELPRHEKNTLLKMLSSFWSERSASGWTPLDYPFSQIEHVWENSDIIIREDEPSSIIALALSCPDYSKKLQEFRDDQTSSDQDYPGSETMEDNIERNLLHEENTNIRYTFTNRGVRAHCKIFYAQSFDALRRKCGVADRFVESLSRCSKWDSKGGKSKSIFLKTLDDRFVLKSLSAIEVQAFFKFGPNYFAFTHENLFKSLPSVIAKMFGLFQVQIKTPTGRDFDWFMLVMENLFYDREPNKRYDLKGSMRNRKIEVTGERDEVLLDENLVDIIYSEAPIFVREHTKKLLKSSVWNDTLFLSQNNVMDYSLMAGFDDTNREIIVGIIDCIRTYTWDKKLESWIKDRGKNKPTITSPKDYRNRFRIAMEKYMLQAPNCWHQFSGRLLGAGPVGVRALEGNANMGTGAGSARHKRMSSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCU3</accession>
    <name>A0A9W8YCU3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cupin type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002778</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCU3"/>
    <dbReference type="OrthoDB" id="3511549at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="2">
        <location>
            <begin position="47"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="145" mass="15616" checksum="6A55BA44829C6D3B" modified="2023-11-08" version="1">MSPPDRVPGEVQVKKGSDIHAPDGPQTDGMLRMPAIVDASDQLCGTVMIAKPRTSSAVHHHGEEDTLVYAAKGHGAIVSGPNGEKRQELVPGDFALIPAYAEHQEVNDSDEDVVWIITRGGRNPIVHNLEGWSKSHEILPEQGAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCU6</accession>
    <name>A0A9W8YCU6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chitin-binding type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_002818</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCU6"/>
    <dbReference type="OrthoDB" id="2019572at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00035">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd11618">
        <property type="entry name" value="ChtBD1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02851">
        <property type="entry name" value="E_set_GO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.10">
        <property type="entry name" value="Endochitinase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.80">
        <property type="entry name" value="Galactose oxidase/kelch, beta-propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001002">
        <property type="entry name" value="Chitin-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036861">
        <property type="entry name" value="Endochitinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037293">
        <property type="entry name" value="Gal_Oxidase_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009880">
        <property type="entry name" value="Glyoxal_oxidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015202">
        <property type="entry name" value="GO-like_E_set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32208:SF21">
        <property type="entry name" value="LOW QUALITY PROTEIN: ALDEHYDE OXIDASE GLOX-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32208">
        <property type="entry name" value="SECRETED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00187">
        <property type="entry name" value="Chitin_bind_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07250">
        <property type="entry name" value="Glyoxal_oxid_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09118">
        <property type="entry name" value="GO-like_E_set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00270">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57016">
        <property type="entry name" value="Plant lectins/antimicrobial peptides"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50941">
        <property type="entry name" value="CHIT_BIND_I_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040999722" description="Chitin-binding type-1 domain-containing protein" evidence="5">
        <location>
            <begin position="18"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="6">
        <location>
            <begin position="82"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="6">
        <location>
            <begin position="152"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="6">
        <location>
            <begin position="248"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="202"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="105"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="123"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="173"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="193"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="268"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4374079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="824" mass="86067" checksum="BEDEEBADD371298C" modified="2023-11-08" version="1">MRATLFGLGALAGLATANINFNWVNPSCEFNTIGANNCLTGQHCTEQNTCVADLKSDKSKLVSRSHLRVPRAKKQTTKYSTDGQCGPANGNLLCDPNSTVYTGTCCSQYGWCGNTPAHCGTGCLSGCSGTAASSVKPAVTPTAVAGTAPRDDGRCGKDFAGATCDANGPYGGCCSSYGYCGSTDGHCLVANGCQNGCKDSTPVVSSKANPAPVSSRPPSTTTSGEPVLGKPSSVASAKASATGVATTDGTCGAKFGNTVCGNWAQGSCCSMYGYCGNTTAHCGEGCQNGPCVNAPGVSAPAASPAPAAPTPGSIVIKGRSGVPAMHAGLMPNGKVVFLDKVENYTDLKLANGQYAYSSEYDPATQKLTALSYKTNAFCSGGIFLADGQFVSLGGNAPLDFIDPTVGDGFKGIRYLKRSSSDSSLDGKGWSEPGQQLNTARWYASVQIMPDDKIFVASGSLNGLDPSKPENNNPTYEWLNADGTPQGKSTNMEILSKNQPYYMYPFMHLMKDGNLFVQVAKSAEIFNANTGSVVRQFADLPGTYRTYPNTGGSVMMPLSSANDWNPDIIICGGGPYQDITAPGDPSCGRIKPLDTDPEWEMDSMPEGRGMVEGTLLPDGTIVWVNGAQEGAQGFGVAQDPALEVLLYDPNQPKGKRFTTGPKSTIARLYHSVALLLLDGTLLISGSNPVEQPILTPSAQNPYVTEFRNEIYTPPYLQGNPTRPSGVTLSSKSLKANGSTFTIKFTAPANAKAVKVSLYYGGFVTHSVHMGHRMLFLDNTGFKAGSTAQSLTVTMPPNKNVAPAGPYVVYVLVDGVPAIGQFVQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDH2</accession>
    <name>A0A9W8YDH2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">AUR1</name>
        <name evidence="8" type="ORF">N0V83_002089</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDH2"/>
    <dbReference type="OrthoDB" id="5784at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070916">
        <property type="term" value="C:inositol phosphoceramide synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006676">
        <property type="term" value="P:mannosyl diphosphorylinositol ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03386">
        <property type="entry name" value="PAP2_Aur1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000015">
        <property type="entry name" value="Aureobasidin resistance protein Aur1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026841">
        <property type="entry name" value="Aur1/Ipt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052185">
        <property type="entry name" value="IPC_Synthase-Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310:SF11">
        <property type="entry name" value="INOSITOL PHOSPHORYLCERAMIDE SYNTHASE CATALYTIC SUBUNIT AUR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14378">
        <property type="entry name" value="PAP2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="335"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Inositolphosphotransferase Aur1/Ipt1" evidence="7">
        <location>
            <begin position="169"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="397"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="403"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4375010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="448" mass="50020" checksum="449CE8ADE5ABED41" modified="2023-11-08" version="1">MTNKSYLAFLDNNAAADMLSQTKVPGLSALPSFIPHRLRRKWRATKSRIRSRQSPTSSIASLSWSCSPSDTLRSLRTHPWSIYDAQYLFLAIVAIFSLSVSEAPGPFAKTFVATLLMTGLCLPITRQFLLPLLPTLTWVLLFSSCKYISADYRPAIWVRVLPALENILYGANLSNILSAHKHVVLDLLAWLPYGVIHYVSPFIVSGCMFIWGPPGTLPIWARAFGYMNITAVLIQIVFPCSPPWYENTYGLAPANYSMKGDAAGLKAVDALFGGPFYENTFLASPMVFGAFPSLHSGWATLETLFMGHVFPKLFPVYVFYTMWLWWSTMYFSHHYAVDLVAGSLLAGICYFFGRAKFLPRPQPDKEFRWDYDYVEIGDPMDGSGYSMLDIYEEFQPQSDSDDWASGSSSSYSTGGRSPLGARSPVDDAQSLWDGDTVGSDTEHSSRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE05</accession>
    <name>A0A9W8YE05_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="12">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Tyrosyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TYS1</name>
        <name evidence="15" type="ORF">N0V83_002411</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="12"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE05"/>
    <dbReference type="OrthoDB" id="197206at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000004">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000040">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050489">
        <property type="entry name" value="Tyr-tRNA_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006330">
        <property type="entry name" value="PRK08560.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264:SF4">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264">
        <property type="entry name" value="TYROSINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="383"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="11"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="415"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4375325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="454" mass="50105" checksum="7EF7E5279B1E3DDB" modified="2023-11-08" version="1">MADPVAIQDEIAVIEKRIEELQTQLKDAKARLQQSREESLASGPGAHLSPEDKMALIKVNLQEVLNPEIMDEALKKQGHLKVYWGTATTGRPHCGYFVPILKIAQFLAAGCHVKILLADIHGFLDNLKAPIELVKFRAEYYRFTITALLKAVNVPIDKLEFVLGSSYELNSDYTMDLLRLASITSEAAAKKAGAEVVKQTENAPLSGLIYPLMQALDEQYLDVDVQFGGVDQRKIFALAKDVLPKIGYKERAHLMNPMVPGLQGGKMSASDPDSKIDVLDTADAVKRKLKKAFAPPKVVEENGVLSFVEYVLLPAGALIYGSPKFVVQRRDAEPLVYTDIKQMQDDYVNDVLIPQDLKPAVTDALNKLLEPVQREFQASKEWQEIETKAYPPPPAEKKKEKKKKDKGAFHPGAARKVEAKADGHVEGVDKDKVDVGTESGEKAIEGLTLKEGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE17</accession>
    <name>A0A9W8YE17_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003132</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE17"/>
    <dbReference type="OrthoDB" id="3788706at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4374391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="144" mass="16211" checksum="1DAE8B80ECE77C18" modified="2023-11-08" version="1">MELRNLKTRTESGKFDDAQYILGQVRGTIGAIRYLSHTGTPEVNGFLTAIINNVGAQWRLSQQVHNANHPNDRTAIGDFWSEWVKDFYANFVIGNARNWAREAIDGLREAWTNSADPGAQQILDALTSLDTQLETLTIDTSLWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEH2</accession>
    <name>A0A9W8YEH2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Long-chain-alcohol oxidase</fullName>
            <ecNumber evidence="5 12">1.1.3.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">N0V83_003411</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Long-chain fatty alcohol oxidase involved in the omega-oxidation pathway of lipid degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>a long-chain primary fatty alcohol + O2 = a long-chain fatty aldehyde + H2O2</text>
            <dbReference type="Rhea" id="RHEA:22756"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17176"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="EC" id="1.1.3.20"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.20" evidence="5 12"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEH2"/>
    <dbReference type="OrthoDB" id="269227at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046577">
        <property type="term" value="F:long-chain-alcohol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012400">
        <property type="entry name" value="Long_Oxdase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46056">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46056:SF12">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028937">
        <property type="entry name" value="Lg_Ch_AO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="16">
        <location>
            <begin position="225"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="15">
        <location>
            <begin position="273"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase C-terminal" evidence="17">
        <location>
            <begin position="590"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR028937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR028937-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF05199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4373120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="744" mass="81081" checksum="32D5182AD5BAAB38" modified="2023-11-08" version="1">MADLPTEPVSPKATPLPPLPSEDPLTPAQWKTLLAITDAVIPAIKPMSTANVRYDIAVADSEYLTAVSKLRGLTPENDPDAEAAAKELLEDYASKDPAFKLELSRLFGMYMPQDQRQGLLMVLNVLNTRVGSLALTGYLTPISEQPAHIRESILQSWANARLGTLRQLHRSLTVLAKQTWIKCTPSLRRVLGVPRVPVGMKPGKGYDYEFIQMPPGEKPEVIETDVVIVGSGCGGAVSAKNLAEAGHKVLVAEKAYHFTPDHFPMTEANGWNHLFMNGAFVSSDDGSVSVVAGQAWGGGGTVNWSASLQTQGYVRREWAAKGLPFFTSAEFQGSLDRVCGRMGVSTEFIKQNRSNQILLEGARKLGWSHKPVPQNTGGAQHACGHCTFGCGSCEKQGPVVSYLPDAARAGAKFIEGFHAERIVFSNKGRKKVATGVFGTWASRDINGGVAGSPLTRRKVLIKAKRVIVSGGSMQSPLLLLRSGLKNPQIGRNLYVHPVTVLGGIHEEEIKPWEGAILTSVVGEYENLDGKGHGVKLEATNMIPSSWLIWMDWKNGLQYKLDAARMKHMAGYISICRDRDTGRVYPDPVDGRVRFQYSTSKFDKKHIMEGLLALAKIQFIEGASEIFTVMPGMPPFLRDESAPAGSGINDEKFQAWLDEIRARGFRMPEFGFVSAHQMGSNRMSAVEKDGVVDPEGKVWGTEGLYVADASIFPSASGVNPMVTNMAISDWISRGIARGLEERPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEN0</accession>
    <name>A0A9W8YEN0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Altered inheritance of mitochondria protein 24, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">AIM24</name>
        <name evidence="7" type="ORF">N0V83_002360</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the AIM24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEN0"/>
    <dbReference type="OrthoDB" id="5295771at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.160.10:FF:000001">
        <property type="entry name" value="Altered inheritance of mitochondria protein 24, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.160.10">
        <property type="entry name" value="Mitochondrial biogenesis AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002838">
        <property type="entry name" value="AIM24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036983">
        <property type="entry name" value="AIM24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016031">
        <property type="entry name" value="Trp_RNA-bd_attenuator-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36959">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36959:SF2">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01987">
        <property type="entry name" value="AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51219">
        <property type="entry name" value="TRAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4375274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="383" mass="41468" checksum="51449344CA60DE58" modified="2023-11-08" version="1">MLRPAASPWKSLARCTRAATTPSTQNAARCINISATSSSTEPTLEPQVNVDASATPVASPDARFEVLGTPFSLLSVSLSASQTLYTRRGTLVALSGKSENTLSTLSFLEPFRRAPVGIPFLYQKVSSTSPVTALVSTKSPISSIVSVNLDGRSDWVISQRQALLAWTGHTLSLKPQYNVKLGIAHWGNTYITGRGLLALVGSGQIYQVQVKAGESYVAHPSNVVAYTASSTPPLPFRFKSSNLRFQIPDLGFGSLVQNVKFFRVMSETGTWRALATTYYTLKTWMRRTIWGDRLFLRFEGPTTMLLQSRASRISDVLTLRDVNEIADSPPGAVQDAVARKIKEEIKEIGAGTKAPIASTDASGTVRYATIRNGKAEFDNKSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEY4</accession>
    <name>A0A9W8YEY4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1989 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEY4"/>
    <dbReference type="OrthoDB" id="504708at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018959">
        <property type="entry name" value="DUF1989"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31527">
        <property type="entry name" value="RE64534P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31527:SF0">
        <property type="entry name" value="RE64534P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09347">
        <property type="entry name" value="DUF1989"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1989" evidence="1">
        <location>
            <begin position="45"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="253" mass="27532" checksum="3CB13B0648B35E5B" modified="2023-11-08" version="1">MRVPIPALQQHEEEAPINPIRNSNTVTAIPTAESFCINRKLTKHVVPAGHGYAFAVKKGTRFRIVDLHGEQVVDLMAWVSSTSNSTSSDSNPPVPTSATLTLEKMSTAYTRYHLRGVTPTLGEALYSNADRPLLRVTADTVKVHDMTFMACFPSLYTSQGLHNHRSCAQNIFEAMQPFGMKDILEIAEPFNCFQNTPNYSLKALGSSRAGDFVEFEAEVGLVIAVSCCPYDIEGFNGGTITDVAIVVSEGMGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF24</accession>
    <name>A0A9W8YF24_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Proteasome regulatory particle subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RPN5</name>
        <name evidence="5" type="ORF">N0V83_002759</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit p55 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF24"/>
    <dbReference type="OrthoDB" id="268763at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000070">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054559">
        <property type="entry name" value="PSMD12-CSN4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040134">
        <property type="entry name" value="PSMD12/CSN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040896">
        <property type="entry name" value="RPN5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12/COP9 SIGNALOSOME COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22241">
        <property type="entry name" value="PSMD12-CSN4_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18098">
        <property type="entry name" value="RPN5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="4">
        <location>
            <begin position="267"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="211"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4374020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="495" mass="57020" checksum="5B3C3D332F3738A6" modified="2023-11-08" version="1">MSSREVLKADKDFTKEVDAAIPEAEKLGEGDAQAGIDKLLALEKQTRQASDLASTSRLIIAIVTIAKLAKDWSLMNEQVLLLSKKHGQLKQATTKMVQTVMGFLDETPNLETKLSVIETLRTVTEGKIFVEVERARVTRILSNIKKEQGDINAAADILCELQVETFGSMTRREKTEFILQQVSLCIQKGDWTQAGILSRKISTRYFARRPKKTPEQLEKDQKEREEKEKNRSIDDPPIEPEDDVTDLKLKYYEQQITLAKHDDKYLDVCKHYRQVLDTEAVEEDPNKLRAILQRVIYFIILAPYDNEQSDLIHRIQRDSRNSQIPQDAQLVKLFTVPELMRWPMVAKQFGPHLTETDVFDAEKDDSDDEKAFKRWQDLRKRVIEHNVRVVAKYYTRIQIPRLTELLDLTEDETEKNISELVTAKTIYAKIDRPARIVTFSKPRDADDVLNEWSGNMKSLLGLLERIDHLITKEEMMARIQAPKMDKSKAAKGKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF67</accession>
    <name>A0A9W8YF67_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001226</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF67"/>
    <dbReference type="OrthoDB" id="3902588at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="2">
        <location>
            <begin position="401"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="183"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="600" mass="66225" checksum="D9D4DAA0BF67DD9C" modified="2023-11-08" version="1">MFPTFDVPPQHAETVGARKARKAKEDGTTRRASSATSHSSGSTRSSTKPVEKSGWSWGSKKKNKEIQEISTLPSARKSQPVQEEPAPEPEPEPSPEPEPSISPSQDWTASLAYRHEEAQRPPAQRFHTEPHFPPPPQQYFAPPPSRSLPSIPPAGALPQRPSPGPDLGLRVPNDAERARSRVSHASSVSPSTTSSDRTGFTGSSRSVFSSNSGYASSGYDSVSSEERNRIDARYASNMQSNMQGNLQSMQNLQLEPVERSSSRSESSLSDRSARAPQGPFARALAKMESAGARIITARLSEEWDGLDDDESYREVMFEKRLWALTAYQRLTQNKQLQSPAHEILSGSRPADQRRILHLHGSLADGWMLATRYPAATVYTTSSNTSFHSPSSHPAPLNHHSLYVPSLATAMPFPDGYFDAIVSRSVSTVLRNDEWARSFFDCRRVLKPGGQIEILSVDAHLNGEGPRLASWVDENIVCKLEAHGLSKLPSDTILDTLEIVGLENIRRARVALPAALSALKTSVRPNADMVDASRMMAFLGRHFYQDLYGKMMQGAPQGEEWFWARRDVRDECERAQTKMVLTIACAQKPMATPHGESYLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGQ8</accession>
    <name>A0A9W8YGQ8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGQ8"/>
    <dbReference type="OrthoDB" id="3509362at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF15">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="398" mass="44348" checksum="EF2855B3EB55589B" modified="2023-11-08" version="1">MPLLPAVSPGPSSGLSPVDSFLFHYWIDHLSTLISVAPRNAHPTPFQLHLTAMAHDSVALRSTILSMAASHLGSVSNDSNLRLQAYRHQRTAIRELQGIIQEPGGTNHEPAFATVMMMQVSSRLFGDDDEANVANHLMGAKAMVAWHREEGWAASSTARFLMSLFAYHDILSSVSRGAQPLVDHATDFTALEGEKNMASIANVLLVVARISELQQTIKRRKLDSTDASLTEEEDAAGRVLQQALLFMDFGDLNHKGLSNEIDVRYTAEAYRHAAFIYLYRTWLDIGAPNPISMDHVQRCLSYIEQVDVNSPLTTAHIWPLFTAGCEAVGDAQRLFVRTRFGDMYNLKKFPSLKRVMRDIEDVWAAKDMEQEMKGLDGMAKVDCIQVILKRRGREVDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGZ1</accession>
    <name>A0A9W8YGZ1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">60S ribosomal protein L30</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RPL30</name>
        <name evidence="5" type="ORF">N0V83_002174</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGZ1"/>
    <dbReference type="OrthoDB" id="1928736at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000001">
        <property type="entry name" value="60S ribosomal protein L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039109">
        <property type="entry name" value="Ribosomal_eL30-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022991">
        <property type="entry name" value="Ribosomal_eL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002172">
        <property type="entry name" value="PRK01018.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449">
        <property type="entry name" value="RIBOSOMAL PROTEIN L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00993">
        <property type="entry name" value="RIBOSOMAL_L30E_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="4">
        <location>
            <begin position="12"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4375092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="110" mass="11836" checksum="20C06683F38E5D71" modified="2023-11-08" version="1">MAPKKSKRTADSINSRLALVMKSGKVTLGYSSVLKSLRTGKAKLVIIAGNTPPLRKSELEYYAMLSKTSVHHFSGNNIELGTACGKLFRCSTMAILDAGDSDILTTNTEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH00</accession>
    <name>A0A9W8YH00_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Interferon-induced GTP-binding protein Mx2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_000183</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH00"/>
    <dbReference type="OrthoDB" id="415706at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF215">
        <property type="entry name" value="DYNAMIN GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="4">
        <location>
            <begin position="28"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="3">
        <location>
            <begin position="607"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4377358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="695" mass="79057" checksum="9B372E74A1CB7F0C" modified="2023-11-08" version="1">MNEKIQPGLGNQTHLNKIDKLRELNVKSLELPQLVVVGDQSSGKGSVLESLTGFSFPQDPGLCTRYATQISCCREAEQSVVVTIIPRAGADAATVDRLRSFRRTIPELTDDIFKKIITDANQAMGIRMSEHDTDSNLQTFSQHVLKVKINGPQQQHFTVINVPGIFRVPSLPFTTDNDVSLVRNMVEFYMQNSRTIIFAVLPCNVDISTQEILKMAETADSDGIRTMGVLTKPDLVTEHVTQAEVKDLVLGKRNQLRLGYYIVKNRSADDKESTISDRLAKERAFFAESTWNALLVSGRCGIESLRPRLSDLLMTISKREFPNVKADVARRLDQCRSELERIGPSRKDHNSQRMYLGRLGSKFQGITQCALNGYYDSESVFANDPSHKRHVTPTWTNDGEDEYNVEDTEDNQAYFDVVLEKHPELHDILDSEDYECPKPTPFKDDPTAEHIERVYHENRGPELGTFSGAILAVTFKEQSEKWEPLVLAHVSKSIMLVHDYISRIIETVCPDKQVREQLWEVLIIDKLRKAYTRAMEHARFLLQIERSGRPSTYNHYFNSEVQKKQMDRLEAIIERQPKTYGKAGNTYASTLNLRSLVGDKNNMQQVCEDILDVLSSYYKVSRKRFVDVICRQVIGHFLLEGDESPLRIFSPELIMGLDTEKLEMIAGEDSETKNRRAMLESEVKSLEVAMKLLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH41</accession>
    <name>A0A9W8YH41_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alcohol dehydrogenase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000810</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH41"/>
    <dbReference type="OrthoDB" id="3941538at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43189:SF1">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C1198.01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43189">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C1198.01-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="2">
        <location>
            <begin position="69"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="243" mass="26304" checksum="A2D02753C2090BF6" modified="2023-11-08" version="1">MGFKGLSGAGGGFSETVAISAKQLYPLPDSVDLSLAALIEPLAVAWHAIALYGTTDWSKQSVLITGGGPVGIAHVYVLRSHGCKQIYISEPTTTRAAQNKEIADAVVNPVTENVGERCRELTGGEGVDVVFDCAGIPKGLEAGFDALRYKGTYMNVAAWTTPMVIPFLPSMLKEITVKYSLAYHDQDFKETVEAFVAGKFKGVERMVTSRIHIDDISKKGFDELVTNKDQHIKILVTPDRTKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH85</accession>
    <name>A0A9W8YH85_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">COPII coat GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SAR1_1</name>
        <name evidence="13" type="ORF">N0V83_000411</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. SAR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH85"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006687">
        <property type="entry name" value="Small_GTPase_SAR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45684">
        <property type="entry name" value="RE74312P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51422">
        <property type="entry name" value="SAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="60"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606687-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4377584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="124" mass="13795" checksum="0F86025087F76EB5" modified="2023-11-08" version="1">MWIINCDRVAVLQPTLHPISGIVFLVDAKDHERLNESKAELDALLNMEELSKTPFVVLGNKIDHPDAVSEDQLRSVLGLYQTTGKGKVPLENIRPIEIFMCSVVMRQGVFPGIDIGILWVGKVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHK1</accession>
    <name>A0A9W8YHK1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002479</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHK1"/>
    <dbReference type="OrthoDB" id="21678at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037460">
        <property type="entry name" value="SEST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37981">
        <property type="entry name" value="LIPASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37981:SF1">
        <property type="entry name" value="SGNH HYDROLASE-TYPE ESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="304" mass="35093" checksum="5F93B28349B44B4A" modified="2023-11-08" version="1">MIGDTTENLLDFSDPVNNQINQVHQDTTFATLSIGGNDILFTKILKVCVFGITGQSNAKACAEKQAESRAQMYSKDFHGRYYKVLDQLITQKFEWTPDTHDTSVIYQTGYIQFFDDFTTQCDDVKFIPVVPFAPKITQDLRRALNLIVHQLNEVLQYWIDLRNDPVTKFLSRKGEMQTFVSPIHWVDMDWRYMDHRFCRDGVKEPNRDDEETWIFHAPAMPLIESQMQNETYLNLIAERYPITWQPGRPDEPGYNDAGVADPDERPFDPNDPIQVLASEKTVRTFHPKPAGFAAEAEELKAALY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHZ2</accession>
    <name>A0A9W8YHZ2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Arp2/3 complex subunit, actin nucleation center</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ARP2</name>
        <name evidence="8" type="ORF">N0V83_001482</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the actin family. ARP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHZ2"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10220">
        <property type="entry name" value="ASKHA_NBD_Arp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000050">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000005">
        <property type="entry name" value="Actin-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4376201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="389" mass="43810" checksum="36F7CBC6AEC5211E" modified="2023-11-08" version="1">MAEVPIVLDGGTGFLKAGYAGQNFPDHQYPSIVGRPILRTEERDGGDIQLKDIMCGDEAAAARSMLQITYPMENGIVKRWDDMQHLWDYTFFEKMHLDPTGRKILLTEPPMNPLKNRETMCEVMFERYGFGGVYVAIQAVLALYAQGLSSGVVVDSGDGVTHIVPVYESTVLNHLTRRLDVAGRDVTRNLIALLLRRGYALNRTADFETVRQIKEKLCYVSYDLELDQRLSEDTTVLVESYTLPDGRVIRVGSERFEAPECLFQPHLVDVEQPGIAEFLFNTIQAADVDVRSSLYKAIVLSGGSSMYPGLPSRLEKELKQLWLTKVLGGNPERLNKFKVRIEDPPRRRHMVFLGGAVLANIMADKENMWISKAEWEEQGARALEKLGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIZ0</accession>
    <name>A0A9W8YIZ0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000588</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIZ0"/>
    <dbReference type="OrthoDB" id="566138at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42678">
        <property type="entry name" value="AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42678:SF34">
        <property type="entry name" value="OS04G0183300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040845934" description="Amidase domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="Amidase" evidence="2">
        <location>
            <begin position="54"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="550" mass="58413" checksum="A8CB05639AA0A51D" modified="2023-11-08" version="1">MVSSWFLLRALAIFAPLTSVLGQYDTPAHLPLLLDATAEELTAGLDAGDFTSVDLVNAYVGRILQVNSTLHMVTEINPDAWTIAKELDEERAQGKCRGPLHGLPILIKNNIGTADEMNNTAGSWSLVGARLPRDSTLAAKLRAAGAILLGKTNLSQWANYRSSNSSNGWSAYGGQTYGAYYPGQDPSGSSSGSGVSASLGLAFGTLGTETDGSIISPSQQNNIVGIKPTVGLTSRSLVIPISEHQDSPGPMARTVKDAAYILQAIVGPDQYDNYTTAIPWASNASNVSEPDYVAACTFDALKGKRIGVPRNAIGRRTASTAPIYDAFEAALSVLREAGATVVDNTNYTAYAEYLNSTAEGTVLDADFAPDLAAYLSELIYNPQGVETLADVRNFTRTFPLEDYPDRNTADFDEALALNFTNTDYEAWQAYEEDLYLGGEGGLLGALSTYNLDAVVVPADYASGISAIIGGPVITVPLGAFPSNTTVVTNRRGDLNATAPNIPFGLAFAGALWSEADLIGFAYAYEQRTNVRKKVHPYIEATIELVDVVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJA5</accession>
    <name>A0A9W8YJA5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PRE6</name>
        <name evidence="8" type="ORF">N0V83_000152</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJA5"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03755">
        <property type="entry name" value="proteasome_alpha_type_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000004">
        <property type="entry name" value="Proteasome subunit alpha type-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 4 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="7">
        <location>
            <begin position="5"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="238"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="272" mass="29548" checksum="224CC73C1C22618A" modified="2023-11-08" version="1">MASGYDRALSVFSPDGHVFQVEYALEAVKRGTCAVGVKGADIVVLGCEKRSAMKLQDTRITPSKICNVDTHVALAFAGLNADARILVDKARLEAQSHRLTVEDPVSIEYITKYVAGVQQRYTQSGGVRPFGISTLIVGFDPNDKTPRLYQTEPSGIYSAWKANAIGRSSKTVREFLERNHKEGMDREETIKLTVKSLLEVVQTGAKNIEIAVMAPGKLIEMLPVEDIEKYVENINTEKQEEAANRRPGRTPGTGTAAILTRPTPAEGSGSGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CGV1</accession>
    <name>A0A9W9CGV1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CGV1"/>
    <dbReference type="OrthoDB" id="3918601at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38794">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38794:SF1">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="189" mass="21097" checksum="C73D618A625A291B" modified="2023-11-08" version="1">MIYLSGFSSSNPTRDLWKWTLFTQIIECITILTSCVPYLRPLLESLPSGLYGSDELRRRGTPSELGYSRSKSGSYQLSSNASKTGEGRRQSRKSETEGGIKRFLPMLSQEKTTHSNSASGLPGGPRRPDGEMDVEITAVNHRLGDDKKWETESTGSHSKILKTTVMSTEWEEAQRKSRDASSDDIEVMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH91</accession>
    <name>A0A9W9CH91_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010951</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH91"/>
    <dbReference type="OrthoDB" id="341730at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26121">
        <property type="entry name" value="HTH_CDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="277" mass="30915" checksum="CBBF90DCEA9FE26D" modified="2023-11-08" version="1">MSPIAPGQLPAELVNLLALHSSFLTALSLHYAHNGTSTPADLRDLTASTTLVWRQRKVTFDDVRLLIGVLDHGPSGKDNPYYLSDYGRGKICLEIKENSPMMGGMTHMNEEGLQDMFKDGLDSLWSQWTKSLQLASRPIAMPKRGRGRPKKIDTQQTRLETFLDDSSMSKFLSQLPLADITTCASLTALAPLQEKGRKRLREFKESVQQGRAQKKTRQSVGKENEPAATAQDQQQAQPTVVQQAKITDFAAVQNQPPRPHPRKARSSQSRTRRALTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHG1</accession>
    <name>A0A9W9CHG1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_010550</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHG1"/>
    <dbReference type="OrthoDB" id="408373at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43329">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="40"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4362457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="331" mass="36292" checksum="1E346A0D34658453" modified="2023-11-08" version="1">MAHTQDQKFSELGLEKRVSEKGITSYQRSLENASYDNPVLVLLHGYPQSAYIWRLLIPLLPSKPLFVPDLPGYGCSAPTHQYDKVSVGLRVIEALKESYSNENESLTGFPPIVLIGHDRGARVAYALHVSTGRAPVLGFQVVGVALFDIVPALSQWQVGDTAAAQTGYFHWSFLANVSIAKPMIMAYGGGNWANSMINRWAGSNAEGLRKLRSGDSMTVYSSFFDQESVIEASSRDYEAGATTDLDFETRAIETGQHIVAPFLLVYSAGCLPKRAKKPILEVWSHPWSAGVHIITSNPIGDGIGHFIPEEAPEETAEALLKWLPALRTQPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI13</accession>
    <name>A0A9W9CI13_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009667</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI13"/>
    <dbReference type="OrthoDB" id="671439at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045136">
        <property type="entry name" value="Iah1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14209">
        <property type="entry name" value="ISOAMYL ACETATE-HYDROLYZING ESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14209:SF19">
        <property type="entry name" value="ISOAMYL ACETATE-HYDROLYZING ESTERASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4363374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="24284" checksum="58DFA6E4DA229493" modified="2023-11-08" version="1">MPEDRLQATQLGGLLVRHGLAIFFGANDSSLPDAPNKQHVPLDEYQTNLEKIITHPKIKEHSPRIIIIAPPPINEHLWWPRDQSNGYERVSRVASTTKEYADAACNVGAKLGIPVVNLWKAFMSRIDFKADAWKFGDPLPGSLSIPQNDALMKLMYDGLHFEPEGYRILFEELMKVVVEQWPDQVPEELPMVFPAWNDQEAWKARERTQTSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI18</accession>
    <name>A0A9W9CI18_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009682</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI18"/>
    <dbReference type="OrthoDB" id="265717at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="212" mass="22299" checksum="4CE1E1D7D1E73C82" modified="2023-11-08" version="1">MHHDPLRVAKRQSNAAQRTLISYLGATSLAAYPLFAAAIPVAVTNVLGGLAARLGSEVPMDGAYQYNGGGVYSSETKWVVLPNALSGPGVVVEAWDFLFSTSPDQHPGNGKEFETLKQVLNQPYLLKDATSRPTGRCQRNTYFFGNATAQVVMRSGNVTLGPAASNVGLTSGVGAVKQESALKGVYEGVRGFSACAQLVGYSTTGLWAWGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI29</accession>
    <name>A0A9W9CI29_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Carbamoyl phosphate synthase arginine-specific small chain</fullName>
            <ecNumber evidence="3">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Arginine-specific carbamoyl phosphate synthetase, glutamine chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Glutamine-dependent carbamoyl phosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">CPA1</name>
        <name evidence="16" type="ORF">N0V83_009877</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer composed of 2 chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CarA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI29"/>
    <dbReference type="OrthoDB" id="434at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005951">
        <property type="term" value="C:carbamoyl-phosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000016">
        <property type="entry name" value="Carbamoyl-phosphate synthase arginine-specific small chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.20:FF:000003">
        <property type="entry name" value="Carbamoyl-phosphate synthase arginine-specific small chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="entry name" value="Carbamoyl-phosphate synthase small subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009475">
        <property type="entry name" value="PRK12838.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF4">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE ARGININE-SPECIFIC SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="Carbamoyl phosphate synthetase, small subunit N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbamoyl-phosphate synthase small subunit N-terminal" evidence="15">
        <location>
            <begin position="54"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4363581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="488" mass="53271" checksum="EFDEEC9E250AFC50" modified="2023-11-08" version="1">MLSQMMKPSTLRSANVLGQTIRNRTQARYLATVQSNTDRAIPTPQRRATPISLERATFTIKNGPIFTGKSFGAKTNISGEAVFTTSLVGYPESMTDPSYRGQILVFTQPLIGNYGVPSAARDEHGLLRYFESPNIQASGIVVQDYAMKHSHWTAVESLAEWCAREGVPAISGVDTREVVTYLREQGSSLARITIGEEYDADEDEAYIDPEAINLVRRVSTKAPFHVSSSLGDMHVALIDCGVKENILRSLVSRGASVTCFPFDYPIHKVAHHFDGVFISNGPGDPTHCTSTVYNLRKLFETSQIPIMGICMGHQLIALAAGAKTIKLKYGNRAHNIPALDLTTGKCHITSQNHGYAVDPTTLSSEWREYFTNLNDQSNEGLIHNSRPIFSAQFHPEAKGGPLDSAYLFDKYIENVQQYKDHQATFSDRNNKPSPLLVDLLSKERVGVHPAAPDVEGHAAGQVGPAVDVGGPVAPPYQPITQKPIAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIJ2</accession>
    <name>A0A9W9CIJ2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIJ2"/>
    <dbReference type="OrthoDB" id="3641682at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040995903" evidence="1">
        <location>
            <begin position="18"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="147" mass="15619" checksum="42696FB0DCDE8901" modified="2023-11-08" version="1">MRSTLFALPAFAAAVLADEVSVTWRHELSSGKTALEIQGADKALLAESCSSKIGSLDFTNVDKHGGGSFTVGDKKYEVLSQPENGAPVCTRIFNDDIAVVECTGVDYTVPAGAATSAEDCFSQAENKAAFRSLKSRSDGRAFRESFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIJ5</accession>
    <name>A0A9W9CIJ5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Autophagy-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_008989</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Vacuolar effluxer which mediate the efflux of amino acids resulting from autophagic degradation. The release of autophagic amino acids allows the maintenance of protein synthesis and viability during nitrogen starvation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Vacuole membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ATG22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIJ5"/>
    <dbReference type="OrthoDB" id="42657at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024671">
        <property type="entry name" value="Atg22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050495">
        <property type="entry name" value="ATG22/LtaA_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23519:SF5">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23519">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11700">
        <property type="entry name" value="ATG22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 8" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="253"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="378"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="411"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="443"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="478"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4364395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="530" mass="59321" checksum="711FAD813CA9F2AE" modified="2023-11-08" version="1">MAANDPGLAPLSAPAEDPTKGAVGVPDEDVPVAPDQFDERYETTRWEIWAYYCYYVGDNGLTLFNFGPTAFQNLMYQAAGDSEILHFMGRDRTINSIVLLSNGLSFAIQVVVFLILGSFADFGSWRPNILIVLSMVAFGIGFGWLGVHTQDDWHIGVGLYIVGLIAYQTTITFWTAAFPGLARNTKELRLKAEEFTEGKITRAEYDFVDMMKRNELSNTAFYVQSIFEILILAVIVGIMFGLKVNDSEANNNWGLSVLIAFATGVWILCAIPWFVLEKRRPGQDPGRTNIVVAGVKQLGYALSQIWQLRQSLAYLAGYFLLGDSLNTTVTVIGTLQNEIVAYNSLQLVYLYIVGIAAQAIGIGAFWRIQKHWGLSTKTMFMVIALGIVLLDGWGMIGIWTQTFGFHKKWEVWLYQVFYGLFVCPWYSYSQTMISEVTPRGKEFLFFSLFSIVGKTSAFIGPLVSSAIIDDTGNNSTPFYFLFALSLVSCAWLFFFVDIGKSRIEQQLFLEREKSIRSSNTSVEDVNLRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIP6</accession>
    <name>A0A9W9CIP6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">N0V83_008991</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + UDP-N-acetyl-alpha-D-glucosamine = [(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9595"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="2.4.1.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:16638"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">In the C-terminal section; belongs to the chitin synthase family. Class V subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">In the N-terminal section; belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="370884at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14879">
        <property type="entry name" value="MYSc_Myo17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000012">
        <property type="entry name" value="Chitin synthase ChsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000017">
        <property type="entry name" value="Chitin synthase ChsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000019">
        <property type="entry name" value="Chitin synthase ChsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000055">
        <property type="entry name" value="Chitin synthase ChsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036037">
        <property type="entry name" value="MYSc_Myo17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF45">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03142">
        <property type="entry name" value="Chitin_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08766">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109715">
        <property type="entry name" value="DEK C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51998">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 18" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 18" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10 18" id="KW-0518">Myosin</keyword>
    <keyword evidence="7 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="871"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="911"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1184"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1579"/>
            <end position="1600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1606"/>
            <end position="1627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1634"/>
            <end position="1657"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="22">
        <location>
            <begin position="1"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="21">
        <location>
            <begin position="934"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="domain" description="DEK-C" evidence="23">
        <location>
            <begin position="1777"/>
            <end position="1832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="591"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="18">
        <location>
            <begin position="651"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="95"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ4364397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1837" mass="205292" checksum="49CF1512B8356AD4" modified="2023-11-08" version="1">MASNPLQTASLPSLAQHQQSDTGLTAALASRYHAHLPIATLSTQGIVAINTCTDASRGVDGGREGSAQQAAEGLAQRAYTRLGQRSEDQAVVFLGESGSGKTTIRGHMLRSLLSYSSTPLSKKIEYANFIFDAFTTTKSLTTPIASKSGLLLELQYNTSTSKYATLLGAQFLAHRLERSRVGSVPTGERTYHVLYYLLAGTSLNEKKHLYLDVVGGDGPAGNRTSIGSNKRWRYLGHPTQLKVGIDDPKGFQDFKNALRKLEFGKESIAGICEVMATILHIGQLEFETGQSTTPGADDSGGYSHEGGESVTMVKNKDTLTPIAQFLGVSVSDLEQSLRYKSKTLYRERVTVMLDPKGARANADELARSLYSLLVTWVMEQMNTRISVLPEQVSNTISLVDFPGFANSAATGSCLDQLLNNAASESLITFCQESFFQRQIQELEAEEISLPPMAFYDNTEAKSGLLKSGNGLLSILDDQMRRGKSDMQFLEAIRKRFDGKNSAILPGSVTVVQPGSNFPTPNTSASFTVRHYAGDVDYSVEGLLEENAELISGDMMHLLKSAQAPFVQQLFGQEALHKMSHPKEKSAIVQASVSSKPSRMPSMARRKADQTGRYGRQLNVFDEDAISDADSNISTSRKDKQTGAAGQFVSALKTIESSLRKANPYFVFCLKPNDRRIANQFDSKCVRQQIQALGIAEISQRLRVADYSIFMPFAEFLGLTQTDVGIVGSEKEKVEMALDDKPWRENEVRVGATGVFLSEKCWLEIANIADVPTYARDPAISEENLLTPAARTFNDPRAGLLSPSPGAYYDDKNGSYFGNRDIDARSEAPSGITSGGDMFHGLEQPKVLDEKATDKKLEEVDVIPVSGSRKRWVFIVYMLTWLIPDFMIRYVGRIKRKDVRMAWREKLAINMLIWWSCSFVIFLMIGFPRLICPTQDVYSPEELTTYNGDDGNDAYIAMRGMVYDLGDFLPHHYPNIIPQKSLLKYAGTDATNLFPVQVSALCNGVDGSVHPGVQLNYKTSNYTDSAANLISTTDLNAQYHDFRWFTNDSRPDWWYEQQVFLKANYMKGRVGYSPQYLSTLADKGNSIAYMNKKVYDLTQYVTGGREVKYNPTNTDRPSETPNVDFMDGSVVDIFRVQAGKDISKYWEGLNLDAKLKADMQTCLDNLFFVGDVDTRNSPQCLFAKYFLLAISILLVSVIGFKFLAALQFSRKTVPENIDKFIICTVPAYTEDEDSLRRAIDSAARMKYDDKRKLLVIVCDGMIIGQGNDKPTPRIVLDILGVPEATDPDPLSFESLGEGQRQHNMGKCYSGLYEVQGHIVPFLVVVKVGKPSEVSKPGNRGKRDSQIILMRFLNRVHYNLPMSPLELEMHHQIRNIIGVNPTFYEFLLQIDADTVVAPDAATRFVAAFLHDTRLIAICGETALTNAKSSFITMMQVYEYFISHNLTKAFESLFGSVTCLPGCFTMYRIRAAETGKPLFVSKEVVEAYQEIRVDTLHTKNLLHLGEDRFLTTLLLKFHSKYKTKYTMRAHAWTVAPDSWTVFMSQRRRWINSTVHNLIELIPLQQLCGFCCFSMRFIVFLDLLSTVVQPVIIVYIGYLIYLLVWSKDVVPITAFILLGAVYGLQMIIFIIRRKWEMIGWMIIYILATPVFAFGLPLYAFWHMDDFSWGNTRVVTGEKGQKVVVSDEGKFDPASIPKKRWEEYQAEMWEQHTIRDDRSEISGYTYATRKPFGAGSIAGSEYGGMPPSRPMSHLDLPRYNNQSRLSLAQSGYAMDSMEMANLPSDDAILIEIKDILQNSDLMSVTKKHVKAELEKRFGCDLSLKKQFIGSAVESLLVSGQLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIY3</accession>
    <name>A0A9W9CIY3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inhibitor I9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIY3"/>
    <dbReference type="OrthoDB" id="5518345at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000005">
        <property type="entry name" value="Proteinase inhibitor I2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052471">
        <property type="entry name" value="PBI_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28288">
        <property type="entry name" value="PROTEASE B INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28288:SF2">
        <property type="entry name" value="PROTEASE B INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="70" mass="7680" checksum="1163387686B8C224" modified="2023-11-08" version="1">MPQYNVTLNKDASKDDLDKAKQHVKDQGGEIVSEFSLIKGFTAKIPDDAVSTLQSNDKITVEADGEVRTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ79</accession>
    <name>A0A9W9CJ79_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_008916</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ79"/>
    <dbReference type="OrthoDB" id="10254930at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036435">
        <property type="term" value="F:K48-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032435">
        <property type="term" value="P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903094">
        <property type="term" value="P:negative regulation of protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031397">
        <property type="term" value="P:negative regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46340">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46340:SF1">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22562">
        <property type="entry name" value="UBA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="5">
        <location>
            <begin position="279"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="35"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="89"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="112"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="135"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="226"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4365297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="312" mass="34487" checksum="9E7518D313F2FD2B" modified="2023-11-08" version="1">MSGDVETLKAMGFPEHRVDLAWKATGNIQDAIDWIEKNQDKSDEELQQDQAGASTTATDEPPALQPGEEAKSLVCDDCGRKFRSVNQAQFHGEKTGHENFSESTEEIAPLTEEEKKQRLEELRQKLAAKRAVQSDQDKEDRKKNEQIRLKATKESQDIKEELQKKERLKEAQAKRAEKKADEDARKRVLAKLEADKQERKRKAEEEKAKRAGLAPPPAPAQAPLATSSGPTTSKPASAYTEARLALQTPGGRVTKTFPVETTLFEVAHALEADGLSVNTFTTTFPKKTFDKSDFGMTLKEAGMVPSAALIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJV6</accession>
    <name>A0A9W9CJV6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-carboxymuconate cyclase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJV6"/>
    <dbReference type="OrthoDB" id="10006285at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75011">
        <property type="entry name" value="3-carboxy-cis,cis-mucoante lactonizing enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4366493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="340" mass="34630" checksum="102A69633EABFC46" modified="2023-11-08" version="1">MEGSTRRAGRTVCVGRRGTSKPFSVSSTVVMTDNPQNLFAVNAGSNSLTMMSIPKDATSLSVIGKPAAIPGEFPNTVAASAKNGLACVGTTGAVAGISCAQFGKRGLGTMDNLRPFDIKQTTPPTGPTNTVSQVFFSTDDSTLFTTVKGDPTVNNTGFLSAFSVQQNQGAACLSTTDTRSSPAGTAVLFGSAVIPNNPSSLFVTDASFGATVLNLDPKHSAIVKSAQAIDGQKATCWATISSATNSAFVTDAGVNRIVEMNINDASIIGKLDLSANGDPGLIDLKAAGNFVYALSPGNGTTKAAITVLDVSGGQGKMKQVQHFQLDGVAGPNAQGMAVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKI0</accession>
    <name>A0A9W9CKI0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007543</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKI0"/>
    <dbReference type="OrthoDB" id="5428890at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="337" mass="38482" checksum="7D4E3B7878662D75" modified="2023-11-08" version="1">MPVESREATSALTEAYVPDFFTAQPALSSHEDLFQMICAIRDEPNRKREELSKRQFQAGTQGVMRSTVMSDQNRSMNIAASVLFLTNCGTSYDCADFVEDGGSSITWRDNVTAKDFVSEAYPKNLHPYFDEASDDYKGADVISTIIATKLVKSGFVFEPTNDLRSHLSITYNKREKVVRIFHGVAVLKEMLLASQINKDSCMIPRTVALEVLYTIHHVLFPSDRASQALLSTLVRKRQFDRSLLQYEMARYRRDDDPDITYSYFGTRFSEIYDEMQDPTPRAGWESWLQKYSSPRYMLMATMIGVFIAVILGFLGLAVACVQTWVSYQQWKHPVKQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKM6</accession>
    <name>A0A9W9CKM6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Small glutamine-rich tetratricopeptide repeat-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">sgt2_2</name>
        <name evidence="7" type="ORF">N0V83_006886</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SGT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKM6"/>
    <dbReference type="OrthoDB" id="2335338at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072380">
        <property type="term" value="C:TRC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.420:FF:000005">
        <property type="entry name" value="Hsc70 cochaperone (SGT), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000207">
        <property type="entry name" value="Small glutamine-rich tetratricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.100:FF:000011">
        <property type="entry name" value="TPR Domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047150">
        <property type="entry name" value="SGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032374">
        <property type="entry name" value="SGTA_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45831">
        <property type="entry name" value="LD24721P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45831:SF2">
        <property type="entry name" value="LD24721P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16546">
        <property type="entry name" value="SGTA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 4" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="SGTA homodimerisation" evidence="6">
        <location>
            <begin position="6"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="115"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="80"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="231"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="320"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="80"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="320"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4367305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="353" mass="36000" checksum="316C106EEE920090" modified="2023-11-08" version="1">MATNQSKKRLALAILDFLQTSQTDGSVAPDDAEQLEVAANCIAEAFKVDVDDEAAKKDALGGQNLLAIYNVYEKLKGKTTATTEGSSSASEARPATPQTPAANAPGPGGPNKTEAERLKGLGNEAMKKKDYDGAIKHYTAALEVIPLNPIYLSNRAAAYSGQGKHELAKEDAEMAVAADPNYSKAWSRLGLAKYVLGDAKGAMDAYKSGMDAEGGGSEVMRKGYETAKKKYEEEGGDVGAPDADEPRGAPGGGGGMPDLSSLAGMLGGGGGGGGGGMPDLAGLMQNPMMRQMAQNLMSNPDMMNNLMSNPRIAEMAQQFGGGGGGGGRGGGGGGGGGMPDLSALMNDPNLADM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLB1</accession>
    <name>A0A9W9CLB1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006638</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLB1"/>
    <dbReference type="OrthoDB" id="7289984at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052184">
        <property type="entry name" value="SDR_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45458:SF3">
        <property type="entry name" value="CHAIN DEHYDROGENASE (ATSC), PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45458">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE SDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4368282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="291" mass="31677" checksum="4A169ACE40A23AF4" modified="2023-11-08" version="1">MSSYVITGAARGLGWEFLNQLSSDSKNRVIGIVRNKAATEKKVAEELSGRSNITVLEADITNYDAVKKAVTDTAAVTGGSLDYLIANAAYLPTWDQYSPIGVLGETPEKLTYEFNKLLETNALANIHLYNLFMPLILKGKGKKVTVITSGMADQDLVNDYELDGAALYAISKAAMNMVTAKFNAQYKNDGVLFLGICPGMVDVGHFNNGMNNLDISLTFTEWTADSRTATPEELAKLGQTVEKFQKYAPHFKGPDNPENSVKAVLSVIRKSSLENGDGGAYLSHFGTKQWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLH0</accession>
    <name>A0A9W9CLH0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006684</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLH0"/>
    <dbReference type="OrthoDB" id="10014216at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1470.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050849">
        <property type="entry name" value="HAD-like_hydrolase_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006384">
        <property type="entry name" value="HAD_hydro_PyrdxlP_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01489">
        <property type="entry name" value="DKMTPPase-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28181:SF2">
        <property type="entry name" value="PHOSPHORIC MONOESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28181">
        <property type="entry name" value="UPF0655 PROTEIN YCR015C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12710">
        <property type="entry name" value="HAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="274" mass="30779" checksum="D9ABE3B8A7399FA4" modified="2023-11-08" version="1">MGVQNAVADLPAMKSDPKFIFFTDFDGTITLQDSNDFMTDNIGYGRDKRRQGNLDCLADRISFRDSFRDMMDSVTKPYDQCIQYLVDNIKLDPGFAEFFQWSLQNNIPVVVLSSGMEPVIQALLHKLVGPDASKMQVIGNNVGARQGKNINEEGGWQIVFKHPESGFGHDKSIELRKYSSLPEDVRPTMFYAGDGVSDLSAARETDLLFAKKGHDLISYCAREDVPFTVFEDWTNILSKCREIVEEKTTVKEAAKEGYEAYKRGDAGVKVNTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLL0</accession>
    <name>A0A9W9CLL0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">R8 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rec8</name>
        <name evidence="5" type="ORF">N0V83_006037</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLL0"/>
    <dbReference type="OrthoDB" id="5427633at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030892">
        <property type="term" value="C:mitotic cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21789">
        <property type="entry name" value="Rad21_Rec8_M_SpRec8p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039781">
        <property type="entry name" value="Rad21/Rec8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006910">
        <property type="entry name" value="Rad21_Rec8_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585:SF70">
        <property type="entry name" value="RAD21_REC8 N TERMINAL DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G02900)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585">
        <property type="entry name" value="SCC1 / RAD21 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04825">
        <property type="entry name" value="Rad21_Rec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein N-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="238"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="417"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="684"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="254"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="435"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="686"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="733"/>
            <end position="745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4368955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="745" mass="80883" checksum="9D39E21BF87667FB" modified="2023-11-08" version="1">MFYSHEILTSRKYGVATIWLVATLGSKSTLKRINRKQILDVDVAKTCQTIMDPVAPMALRLQGNLLYGVSRVYLQQCGYVLSDAQMARNTMQMMLRTVENAALDPDAGKARPEQLVLPDDPSFLPEFALAPPELLADLDLPFDLGITRTGESQTFTPFGSQHSQSSHGNAIGGLVLPSSSPSMPRGEFRLEDDNEPGSIGGPSGMLGAGDMLEVLEPDFTFGEDGDIIDLTAGNAVARTPGAPGRNTMHSDAGASERVRREHEEGRRTDAQFPGDQMDIDFPIYGDDLPEGEAFSSSANPQGSEQVEVLHSTSTASAPMRRKRRTARALPTDATIELRNKDLADWNANYLENMKAATQHKIKSRAGPIAKKNAEYFVWGSGIGGIADHMLGTKGSNPFAMFIGDNLFELLTGMNRKKAAGKKHDRDSGIDDATQEESRRVRQKTGEPEEQIGRGLDEDDLFMAGGDEVELPREAETALDDQHIFSAMPWNISASIRGSSAIPRSGRVAMLGSADQNKRGSRMVSASPLLGRGQPGGLQDFKALESDIDFAYGGDEFALLGISSDFPEPEAPLLATTRVRDALSAEGENFLTFVTEAIAEKRNRAQADLEPMSDFLQAEAAADIDEITFEELLPPSENTKVIACQGLMMVLALGMKGMLDVQQPEGFGEIGLKLTEKAKAMQVVEISDGEESESEESEADTAHEEEEVQGENIQEEGVQEEGYFQEQFASGSTAHEDGDHDSLYGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLL4</accession>
    <name>A0A9W9CLL4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005933</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLL4"/>
    <dbReference type="OrthoDB" id="3009728at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="337" mass="37679" checksum="9F4E62907BD04447" modified="2023-11-08" version="1">MQLRSSTVCQPTRTRPCNTNSGAQIGILDKSDEVTKSEFGVVAVMLGLLPGVLQLVGPKFSDLAILATRRPILALFLTVGSPCPALDYETTGMESLLVEDSSPFWPAWLRTRRPWLSVVISLLEYFVVMVAAGNVVYQFYRLTFYAVVLISTQMGQYGGTLEAFAPLLWVFLGIPIHALGVLQLHWCRVSSADNAALTHPRNKTRLITLLQNELTPCAFATDIRSDEIKIQNGLIYQILNWMIKLCIWADVIYGIIVLSTILFVPMWSAIWMIGLIFTGTLLCRLVLAFEMHGLREVPKVKEKASKESMEADYEMARLIPENGKAPLQEHTLPLSHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLR7</accession>
    <name>A0A9W9CLR7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_005949</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLR7"/>
    <dbReference type="OrthoDB" id="5086500at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052374">
        <property type="entry name" value="SERAC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48182">
        <property type="entry name" value="PROTEIN SERAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48182:SF2">
        <property type="entry name" value="PROTEIN SERAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="181" mass="20201" checksum="ACBBD6E7C3FD4E5D" modified="2023-11-08" version="1">MARLEHPDGPLPTRQRTHHSHAETFSSRLSRAPTLSPTEDSFSINEEHEQLKAEDTKKSSFMRRRLASARPQGTKGVESKGPLGLRSLFHAPETLVDLIFVHGLGGDSIKAWCLDNDADLFWPQYWLPKESGFLNTSIHSFGYDSNGGSAKLSTLNVHDFGDSLYDELLNSLHVTNKSKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLW8</accession>
    <name>A0A9W9CLW8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UBX domain protein Ubx2</fullName>
            <ecNumber evidence="3">1.1.1.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ubx2</name>
        <name evidence="3" type="ORF">N0V83_005123</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.8" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLW8"/>
    <dbReference type="OrthoDB" id="270602at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141152">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02958">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14273">
        <property type="entry name" value="UBA_TAP-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006577">
        <property type="entry name" value="UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF6">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13899">
        <property type="entry name" value="Thioredoxin_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00594">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="447"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="530" mass="59264" checksum="BFD7ED52D7BA157B" modified="2023-11-08" version="1">MEEENILNFVGITSCDPDKAAQYLRLTDNNLEQAIQLFFDSPNLDFGPASTSQAPTSNAASSAQNPIHIDSDDEMSDFDPAAHTSTPPARPPPDTEDDEAMARRLQEEMYGGGGPGGAQGVDPDEVRAPMQRTTETLVGPDANWGPPDEDEMHDMVQEQLARRRAGRAGIFNQRTTQTNVWDNTADSSARRRELATATGGASEQSSKMSMLAELFRPPFEIMYQGSWEKARDMGKEEEKWLIVNIQDPSIFDCQRLNRDIWKNDDIKATVRENFIFMQYAKDDPRGQQYINYYFHARDSSDAYPHIAIVDPRTGEQVKVWSGPPIPEPVDFHAQLHEFLDRYSLDVSAKNPIAKRKAETKSKDVDRMTEEEMLEMALQNSLENGKGPKDDDPDALTKSFDNIKGKGKVQEAAPEPEAEPEQEANGSSNPVFAGISSHNPHMEPTVTDPKITTRIQFRGPSGRPIVRRFNLSDPVRRIYEWIKADAPWDGKQGAEFDLAFMGKNLLEHLDSTIEQAGLKGASVMVEFIDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLX6</accession>
    <name>A0A9W9CLX6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DUF1687-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_006145</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative mitochondrial redox protein which could be involved in the reduction of small toxic molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FMP46 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLX6"/>
    <dbReference type="OrthoDB" id="59229at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006660">
        <property type="entry name" value="Arsenate_reductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012882">
        <property type="entry name" value="Fmp46"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28071">
        <property type="entry name" value="REDOX PROTEIN FMP46, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28071:SF1">
        <property type="entry name" value="REDOX PROTEIN FMP46, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07955">
        <property type="entry name" value="DUF1687"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51353">
        <property type="entry name" value="ARSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="41"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4369062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="16299" checksum="8414FC988E8CF5BE" modified="2023-11-08" version="1">MFKRLFGEHGAKNVVTLFHKPSSQASTRVLTLLKQTHAQSVAHATEDQASSHHAQDRAERTEFELDVTEAAPTTDQLKNILEYLGGSSAAGKVISGASNESDAMRMLKADGNAFQRPLVVDWNQGKAVAGENESEILNLIRSVPKETDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMB6</accession>
    <name>A0A9W9CMB6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005242</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMB6"/>
    <dbReference type="OrthoDB" id="73919at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47800">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47800:SF5">
        <property type="entry name" value="FER-1-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="58"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="425"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="506"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="576"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="528"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="585"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="644" mass="72686" checksum="2C84E5C0FBF8E335" modified="2023-11-08" version="1">MPEKEDSRPADADADADAEAQPQNGHAPGAAEKNEAPNGADGQPPKPKKTGSNASKKKDPEGGFDKTPIPRAPPGYTVKFTFHRANHLPLADINTLSADPFIMAELNTGLPTRHKEDPPLTVRTPTIRQTTDPVWNCEWIVANVPSHGFKLKCRIYDEDPADHDDRLGNVHVDVGSLNENWSGIKEQTYPIRKRMVSKRAWLLRAFAVCFGKAEHMNGTLTVSVEVLGRTQDENGGRAYTVGPQFWIRHYSPLLGRLLGQKEPNDGPNGRQSRKTGKQKPEKYNFQSNQMQLRGPVPEAMYHRYVEFKPFVKSMFTAKGVRGFILSKALHHQHARVYNFDSATEFKVLQEPCKDFTQKFLELVHFDKGGRIFTYVLTLDALFRFTETGKEFGIDMLSKHTMHSDVAAYVAFSGEFFIRRLKHHARAPPDEGGQNKSHPPDDIEGGPPDEDVKVKDPSYYELVIDNDSGTYRPNAALLPQLKSFFESNFPGLHVRTLDCQGDAEKMGAMKNEQRERKKKEGDHVVYTQMSRSSSMSSSDVEDLDRLENEGHLPQKHLRQQVQREFGAAADAKMAHMKDYKPSSNHEGWRGDQHPKGKDDGEVERLRRRKGRRGLGRGLGRARARRVDRIVFWTSSPLLFLLASCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMN1</accession>
    <name>A0A9W9CMN1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutathione S-transferase kappa</fullName>
            <ecNumber evidence="4">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_005065</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GST superfamily. Kappa family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMN1"/>
    <dbReference type="OrthoDB" id="4664297at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000096">
        <property type="entry name" value="Glutathione S-transferase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001853">
        <property type="entry name" value="DSBA-like_thioredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051924">
        <property type="entry name" value="GST_Kappa/NadH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014440">
        <property type="entry name" value="HCCAis_GSTk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42943">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42943:SF2">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE KAPPA 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01323">
        <property type="entry name" value="DSBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006386">
        <property type="entry name" value="HCCAis_GSTk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DSBA-like thioredoxin" evidence="6">
        <location>
            <begin position="6"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR006386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR006386-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4370544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="220" mass="24589" checksum="955DFA7801A6AA0C" modified="2023-11-08" version="1">MAKPRITLYVDIVSPFAYIAFHVLKNSPVFKQCEITYVPIFLGGLMHACGNKAPLSIKNKDKWINVERLRWAKYFNVPVSQNAPPGFPNNTIAIQRTLASLSLSHPQSLESAIALFYENFWVHWNDPLKPENMQAIIRTVVGSDEEARKVIEKTKSEEVKKALVGNTNAAFDGGAFGLPWYTATNSNGETESFWGVDHLGQLADHMGFERPGGKGWRALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMS8</accession>
    <name>A0A9W9CMS8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Large ribosomal subunit protein uL3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">60S ribosomal protein L3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">RPL3</name>
        <name evidence="18" type="ORF">N0V83_003900</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the large ribosomal subunit. Interacts with DHX33.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMS8"/>
    <dbReference type="OrthoDB" id="1611972at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000079">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1430.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.960.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.960.10:FF:000004">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000351">
        <property type="entry name" value="Ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.960.10">
        <property type="entry name" value="Ribosomal protein L3, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045077">
        <property type="entry name" value="L3_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044892">
        <property type="entry name" value="Ribosomal_L3_dom_3_arc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_uL3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 17" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9 17 18" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4372127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="392" mass="44387" checksum="5202BDD21EE82129" modified="2023-11-08" version="1">MSHRKFEAPRHGSLAFLPRKRAARHRGKVKSFPKDDPKKPVHLTAAMGYKAGMTTIVRDLDRPGAKLHKKEIVEAVTVIETPPMIAVGLVGYIETPRGLRSLTTVWAEHLSDEVKRRFYRNWYKSKKKAFTKYAKQHSESSGQSITRELERIKKYCTVVRVLAHTQIHKTPLKQKKAHLMEIQVNGGSVADKVSYGHGLFEKPIEISSIFEANEMIDVIAVTKGHGYNGVTSRWGTKKLPRKTHKGLRKVACIGAWHPAHVQWTVARAGQMGYHHRTSVNHKIYRIGQGEDEGNASTDFDVSKKQITPMGGFVRYGEVKNDFVLLKGNCPGVKKRVMTLRKSMFTHTSRRALEKVELKWIDTSSKFGHGAYQTPAEKRQYEGVLKKDLQTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMZ1</accession>
    <name>A0A9W9CMZ1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_003824</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMZ1"/>
    <dbReference type="OrthoDB" id="331544at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008460">
        <property type="term" value="F:dTDP-glucose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05246">
        <property type="entry name" value="dTDP_GD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000304">
        <property type="entry name" value="UDP-glucose 4,6-dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005888">
        <property type="entry name" value="dTDP_Gluc_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="4">
        <location>
            <begin position="62"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4372051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="432" mass="48850" checksum="8E37C88855E32EE5" modified="2023-11-08" version="1">MSALQSPDGTDTVSDSFHGIKRRRGSTILNPRSYVKEELWKQAPVLTGSTKFKPLLGTKSILVTGGAGFIACWFVRHLVLTYPHYHVVSFDKLDHCATLNNTRILNSRPNFTFEQGDITSPTDVKRVLRKYKIDTIFHFAAQSHVDLSFGNSYEFTNTNVYGTHVLLERAREHVIDRFIHISTDEVYGDVPVGAAELSETSILAPTNPYSASKAAAEMMVSAYRSSFKLPLITVRANNVYGPHQFPEKIIPKFIMLLQRKQKLLLHGDGSPTRRYIYAGDIVDAIDTIFHKGDVGQIYNIASKDEISNTDICHRLLGVFGIPHQTQAELAQWVEHTEDRPFNDQRYATDGSKLTALGWSPKMNFDEGLRITVDWFRRFGEVWWGDISRVLTPFPVVAGNEIWTKEEHDDLPSSDGEVLYEEDGASLMKKIRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMZ4</accession>
    <name>A0A9W9CMZ4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_005231</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMZ4"/>
    <dbReference type="OrthoDB" id="440553at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF43">
        <property type="entry name" value="MULTIDRUG TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G03040)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01036">
        <property type="entry name" value="TCRTETB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="394"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="428"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="498"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="32"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="534"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="534"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4370710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="586" mass="63101" checksum="A8C786615BEA28CE" modified="2023-11-08" version="1">MAEDKETAAASDLTPAATSAFPNISPATLHTIIAGLWFCLFISALDTTIVTTALIKISSGFNALEKGPWLVTAYLLTYNSFLMITAKLSDIWGLKILMLSCAVMFLIFSMACGGAQTMLQLIVFRAFQGIGGSGLYSLTFVSIMKLVSREKIGFYSGIISSVFAMANLLGPLFGGIISDHTTWRWIFFLNGPVVAIAIALLFFSLPTLKDGKTNSERIRNLDGIGGLLSICWPIPLIFALQEAGGSHPWSSGVIIGTLVTGIVLLIAFVLYETWVTYRTKKEPIFPIHFLRNPAMGLVLLSMFLLGMPFYVIFVQLPQRFQVVNATSAERAGILLLPATVVSPVGAMAAGLVAKRVPVEFVLILATAVVCIGIGLLSSLPTSSHLWPGVYGYEVITGLGLGLASPPYFMIVGTSIAERDISVGTGALNMMRTLGGAVAVAICTAIHREYLNDKLPAFLSPEQVIAVQDSSTSIAKLPEGVRNQIGNTFGGSYNRQFKIMLAFTGLNFLVAILLALVRKRMGIFNAVPERREDNEFVKAAQKERSDEEAKGGNEKESADFDAAQSARTKHKSDDIQTVEEGTSTKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN42</accession>
    <name>A0A9W9CN42_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN42"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="172" mass="18790" checksum="90E8D62CA26DB667" modified="2023-11-08" version="1">MHAGTEALGVEDDGVGLWLGLGSELGVADGDALDVDDADEVGVSEDWVDEAWVDENEEIIEEDATEEESAEDGIAEEDSEDEDAAERDEAAEDREDEELALLLDKTCEVEDGLALLLLETTELDDESVLLLDAVLELLEDELARALVELEGLIDEDETELHCPKPDWQPVPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN76</accession>
    <name>A0A9W9CN76_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA replication initiation factor cdc45</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CDC45</name>
        <name evidence="7" type="ORF">N0V83_004723</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC45 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN76"/>
    <dbReference type="OrthoDB" id="10258882at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902977">
        <property type="term" value="P:mitotic DNA replication preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003874">
        <property type="entry name" value="CDC45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10507">
        <property type="entry name" value="CDC45-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10507:SF0">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 45 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02724">
        <property type="entry name" value="CDC45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="204"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="751"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="257"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="751"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="778"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4371505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="868" mass="95551" checksum="E3AF51ADAD1FD3E1" modified="2023-11-08" version="1">MPSASDMYLPRNLIAHLYQNLIKRTHAAAPPVLLLVALEPDALCAGRILTALLKRDYIPHKIQPIAGYGDLSRAAEELVRPMRTTDGGSGGVVVCLGVGGMVDLEEILGLDVDEDGQGGSGDVEVWVLDARRPWNLANVFGSPISEDPATGELVRRRAGVDKGKVLPSYQSSRGGIIVFDDGDIEEELQAERAAYCGLEEMPEVGEEEDLDDNSDDQDEEEPSSAQGPKKRKSWSEDEESGDDMTDDERPRQRRRSNSGGSIPSSPEQRPARRGLMIENQLGGSSDFSRSDSRSRSVSPSIAPPKEQSVKSLRRQLIQTRNKYTNVLDKYYQLGTSYAEPVSSLVYNLASELGREDNDLLWNAIVGVSSLELYGRTGSGVGLNPLSAQGGSAGWNGNRGEQIRSVLRDEVRRLNPITDFANGARDAMMGEASGVIPTSARSATDKSIRLSPEPRFLLLRHWSLYESMLHSPYLSAKLHIWSDAGQKRLAKLLAKMGVSLSECKQRYTHMDMELKRGLRERLLKFAPHYGLDGLVPPVGRSGDLKDGWGFVRCWGWKACLSAIDAGVILGAILEVGDAKNLNHSSFDTSNFTSTQDAHQNEVASTQEQQNLAQEHITSRFWTAYDALGSIDLLVDHITTAQHLHRAILRTGTALIEKRQIRHLRAFRMAVVKEGPDVPLFTHPGALTKLALWIAEAIVELNGTKGKNKGSELVMAGLDDSRGLYVVVGLGGGGATESAKERLLKREARIKAKEGRREKRRKEREGKREARRQRNIAAGLEEDEVGDDTEEEEEDSEEDSEDGDEDDSDAEDLDAQRGSGRNRFGNAFQEVVRETGARVRMDSFEACVIEIKKEDLSGFLERLSMKAVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN95</accession>
    <name>A0A9W9CN95_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_004170</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN95"/>
    <dbReference type="OrthoDB" id="10027823at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520:SF5">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="334"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="372"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="25"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="478"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="482"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="506"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4372396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="521" mass="56482" checksum="CAB2559DD8211002" modified="2023-11-08" version="1">MPPLPILSRISDFLGVKSLNATGRDAYIIIILRSLRMFTAGIPSLILALFFSSLKFPDSRIGIFMTLTLLGDVMLSLLLTLVADKLGRRRILFMGSVMMAGSGVVFALFENFWILLVAAVFGIISVTGADCGPFRAVEESILSGLTDEKTRSDVLSWYVTATTMAGAIGAEVAGRTVQVLEKSSWDLKKAYHALFWGYAAFGVIGSILCLGLSNRCEAAGEKRAEMKERGRGGGDGEEEEVLLEAMTPSTTDGFESHDRARSSIQEDLRVKPAQSKKQSYFSQISKPTRNIMYKLWILLAIDSLADGMTPYSLMNYYVDQKFHLSKSTLGDITSASQFLCAVSAVFAGPLAKRIGLINTMVFTHLPSSIASAFIPLPGTVGPTIGLLLFRAALNSMDQAPRSAFIAAVVKPEERTGVMGITSMLRTLAMSVGPSITGLLSGNGAFWVAFLASSICRITYDLGLWVLFVNVKVDKDVGRREEEDGDSVDDDAWNGLLSDTESESSSDEGRKSKDLDRGESRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNU6</accession>
    <name>A0A9W9CNU6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003994</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNU6"/>
    <dbReference type="OrthoDB" id="5316527at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="436" mass="49164" checksum="9933A03B3F5BAC22" modified="2023-11-08" version="1">MFKPQVLRRNFSLFNRVPYSRIVRSAARDGHETFTIQRVHIRKPFLSKSRLVGAVAIAGATYGLGRYLGLEVEIEEVEEEQQKSTRSIDQVSGDDEHYQPAEDFNNEEESDDEYDDALIFLPTGFSRPRPRTYYKGSDPEWKEFRKLALDRPRVEKIRGIAHAATGGITSTNGCVGQLVGMIRDLVAKDPKNVLMLGKIDKSKGKVWIEFQFPDGPPLEYERPGIELTEDLEWRKATRPVEDIHHIHLKRLLFPTEAATALYQDTKRKAELIWRGFKAYIGWEEKYEPETVQQLVQRIGASPSSTSPPAKPMKPAVPNPSPVTPATDTQQPSVSPSVAPADGPAKDVGFVLPDPKKLTLDLSQFRQDFRKSFGQLPMQVPRGSVIVRGLIEVYGERARLTLNVVAVYDPKSGRYTGVRAHLFNHLIPHRQHPKGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNY9</accession>
    <name>A0A9W9CNY9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_002569</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNY9"/>
    <dbReference type="OrthoDB" id="6770063at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF60">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="388"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="469"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="496"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="529"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="562"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="158"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="33"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="77"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4373830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="598" mass="65775" checksum="7C50E1CE9CB597CC" modified="2023-11-08" version="1">MESSQRKARSRSRSRARQPDGPPLHRLLSTQFPDDHSVYHQEDGEGLHVDESARSIHKTETERRGTESSSSDDDDSLHDVDEKKETSEGKDEAALDDERTYEEIRGGIPYEHDVEAKPPSLKKKTSSKSVKDPNLVTWDSPDDPANPKNWSMKRKWAATFIVSSFTLVSPISSSMIAPALTSISAEFNIKSDVEASLTLSIFVLAYAIGPLFLGPLSEIYGRVIVLQIANLFYLAWNLGCGFAQTSAQLMVCRFFSGLGGSAPLAIGGGLLSDCFLPEQRGRAISIYSLAPLLGPAIGPIAGGFISQNTTWRWCFYATTIFTAIVQCFGVLFLQETYAPKLLTAKRDRLRKETGNMELHTEFDSPDRTLATTLKTAMKRPFKLLGTQPIVQVLACYMAYLYGLMYLMLSTFPMLWTGRYGMSDGIGSLHFIAMAVGFFLGTQITAPLNDALYRRLKKRNNGVGKPEFRVPIMVPASLLVPIGLFLYGWSAQEHTHWIVPDIGACLFCAGVIIGFQCIQTYLVDSYTRYAASAIAAATVLRSLAGFGFPLFAPAMYKALDFGWGNSLLGFIALGLGVPAPFMLWKFGETLRGKSKFAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPA0</accession>
    <name>A0A9W9CPA0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002946</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPA0"/>
    <dbReference type="OrthoDB" id="5328412at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="555" mass="60441" checksum="2D4E22011207AE5B" modified="2023-11-08" version="1">MPPKRKDFLKPKQKGKAKAQEPQSENDFLEAADEFEQAAGKWRAGDAAKATRFFNRAIDTYNEGLKRYPRSFDLAYNKANLEYNITEDERIVSQLGNRTALLEETLKSHRFAISLNPSNTDISFNTAQVLTSLAEALLEAGTQADARYEARPLLEEAVDIFTRCLESQQQEYAQMQDEIAKAQASWEGERQQQQQADPEREPERGEDMDTTSTSSEAPGDWATVEEPVTPETILDTCTVQLAALTTLLGLYDPNTDLGSIEKKAQDGLETASTRIPALIELVEKNPRSNNEPEQQSGPTLSIGPASTTEEDVTTPREDAILAAANFQASIAEVTYRSGRSTSTAYAQTVEQTYSSVLENARNGTAPDLASINIQSAYADVLMDLASALADSTQHTASMPVLSPDIEVQWTALTQAQTLLTQLSSAPYTSFLSPLRLADVFLARGDIDLFRFRLSLFAAAKPAWTKSQPVLVSNSGVFYRGARTYAEKAGAAEVQKTADAKAVVAEILKGVASGSVAGTREHWKGKGAAVVKVLQQMVEEGVVGQENAEGVLKLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPV8</accession>
    <name>A0A9W9CPV8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002377</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPV8"/>
    <dbReference type="OrthoDB" id="416217at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040815318" evidence="1">
        <location>
            <begin position="21"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="235" mass="26635" checksum="91D604DC2C451C18" modified="2023-11-08" version="1">MPVWATGVFLGAASFWYVESPDVNEVWPLKPSDPSDLTWMKLGEGKKTLWRVADPTRPESLFSTIIREKPCSTLPDWMETSNTSRISPRVKQALDISPASTVENNVYHLPAIMLSRIQDLRLTQENALKFLYITAFVTPEFLALLESKDKRSVFILGWWFKMMRDGELWWMARRAKVEGEAIRVWLQREEPALAELLDSLGRRKFVEGDGMAPQSPGPSHNAVWGSAGLSAIEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQD5</accession>
    <name>A0A9W9CQD5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_001382</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQD5"/>
    <dbReference type="OrthoDB" id="8048523at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034488">
        <property type="term" value="P:basic amino acid transmembrane export from vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF34">
        <property type="entry name" value="LYSOSOMAL AMINO ACID TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="297"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="318"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4376101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="376" mass="39882" checksum="741D5857F94DC681" modified="2023-11-08" version="1">MHQVWNDAGNTAKGSEPANNSSPTDKKDVPANAAENVLHSKPMDTFRIPNFARSSTPAKESWQESPSGTSPNPHVRRVAASSSPMPSPKTVLYISLILAVLSNTSTATPVSPFAPVSYRIAARTATSEPSLSASELAGKVFSWMSTFLYLGSRLPQLYKNQVRKSTAGLSPTLFAAAFFGNLFYSSSLLTNPCAWFDYAPGEGSGWVGPDGSVRKDWVLRATPFFLGAAGVLIMDALVGLQFVYFHDNDGPRGRSALPRDDEVIITVDDHGRPMRRRLHWRRVSGWMRGWAPAVSVAGTPSASRAATPVDTPRDERSPAASVAGSAASSSQSIPIRGKGSALDEARALLGTSRHASPRSYGAVGSPRSPAALGGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQD6</accession>
    <name>A0A9W9CQD6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sir2 histone deacetylase Hst2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">HST2</name>
        <name evidence="11" type="ORF">N0V83_001981</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQD6"/>
    <dbReference type="OrthoDB" id="420264at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01408">
        <property type="entry name" value="SIRT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF6">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACETYLASE SIRTUIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="10">
        <location>
            <begin position="201"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="158"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="474"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="522"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="175"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="189"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="522"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="331"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4374903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="580" mass="64797" checksum="13A9E4606C7317DE" modified="2023-11-08" version="1">MIILLAIWTIGIGLMWIRTHIIMQRRGREYLAGEHKAILELANAMQDQLTDVDAEGIKGVQTLNETELRCRITKDLCGGSIAYTTPLLANGENGYGDSGWRLRAFFKREGWWLFFGLCSAAGSTTLGFLFPYVFIFIWLIGLTVAIVLTVSMGTTRRSKAAQQDKPWRNSEKGRSKGRHRGKRGKMGNEESTMVDPSTPPQTLSARSLEALAQYIQDGRAQKIVVMVGAGISTSAGIPDFRSPETGLYANLARLNLPYAEAVFDISYFRNNPEPFYTLAQELYPGKYRPTITHSFINLLHQKGLLLKLFTQNIDCLEREAGVPADKIVEAHGSFAKQSCIECKKSYPEERMQEAIHKKWVPHCLDLSCNGLVKPEIVFFGEQLPAAFFENRNLPSEADLAIVMGTSLSVQPFASLPSFCEDRTPRLLINSEQVGDLGGRRDDVLMLEDCDSGVRKLAEACGWLEELEALWAKTARDDTPEKPKEKMKKSRDEALQDEVDKLTKEVEESLRLGKAQHEWLDNHLDNKIARKQEQEAERPGTQPTDDSDSRKLAPVATSSTTKTDPGGGLGHVFPFMKKPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQK5</accession>
    <name>A0A9W9CQK5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_001625</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Component of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQK5"/>
    <dbReference type="OrthoDB" id="433924at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017984">
        <property type="entry name" value="Chromo_dom_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008251">
        <property type="entry name" value="Chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051219">
        <property type="entry name" value="Heterochromatin_chromo-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22812">
        <property type="entry name" value="CHROMOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01393">
        <property type="entry name" value="Chromo_shadow"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00504">
        <property type="entry name" value="CHROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00300">
        <property type="entry name" value="ChSh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="5">
        <location>
            <begin position="46"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="95"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4376342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="230" mass="26173" checksum="225943FA4FED1ACF" modified="2023-11-08" version="1">MPPALSDESESSGDEKIPAQKKGKQPAKQAAPEEIEESEEEAEDEYVVEKILGHKFVKGEIVFEVKWQGYDDPKDRTWEPEANMDGAIDVMKEYFEEIGGRPEPKGQKRKSRPSGVASESGTPASSTKRAKQEKAWSPPPGSWEHEISHIDTVEESLNPKTGNRELFAYLVWNNQKKTQHPLRHTYQKCPQKMLLYYESHLVFTKTDDVVNGDDNTNNDEDENVSMKDSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQX9</accession>
    <name>A0A9W9CQX9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MFS general substrate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_000230</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQX9"/>
    <dbReference type="OrthoDB" id="196103at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051617">
        <property type="entry name" value="UNC-93-like_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294">
        <property type="entry name" value="ET TRANSLATION PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294:SF4">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4377405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="499" mass="54514" checksum="18C9CB459AD00FC4" modified="2023-11-08" version="1">MSEFDAAPPNTYAGGKDNALRTKGNDPLVAPPSHEIADERMNMEEYARTVPKWKRVWQHSLTQMLLLSVQAFCGPAMADAIAGLGGGGLATPQTSNIATAVNYTMLAVCCALGGPIVNKLGTKWALVIGACSFPIRGSSYYCNSKFGNQWYLILGGFFTGIGSGTWYVAESGTIMSLAPANSRGKYLALWIVARNLGQLVGGAINLSKNHMKGAVGGISPDTYVAFLIIECMALPFAFLISPLERVVRSDGTKILVSEKIGTKAEFKLIKVTMTSKLILLSAFWAMWSFFYSGTWSTYLGTYFSVRTRALSSLISPFFCIVGCFGLGFILDMKRFSQRRRAQIGLFTVVVLNLGVYIWSIVMQVKFNKHKPGKIDWEDALYAKSFLPYFFVQTTGPLSQSYMYWLLSSFATDAQANVRNGAAFRCLEAVGQAISYGMNTQIKTSPLIGFCVTFGMMGLAVGPMLMLVNQTPDRIPADVIAEEQEREQNKVGAIEVEAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQZ5</accession>
    <name>A0A9W9CQZ5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_001157</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-binding monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQZ5"/>
    <dbReference type="OrthoDB" id="74360at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051209">
        <property type="entry name" value="FAD-bind_Monooxygenase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42877:SF7">
        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42877">
        <property type="entry name" value="L-ORNITHINE N(5)-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4375879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="612" mass="70624" checksum="EC73F67FACDA63B8" modified="2023-11-08" version="1">MGIQADPRSLYAAPGIAADENVKLRDQLPPETDQYGYRIKEQPMGTKRQVKVILMGAGASSLNFFKKAEESMENMDMTCYEKNSDVGGTWLENRYPGCACDIPSVNYQFSWKIKLWSHYYSYSPEIWAYLKSIERENDFIEKYIKLRHRIERVEWDNVAGLWRCTIRNLETNEVFDDSAEFFINAGGVLNNWKWPDVHGLHNFKGKLMHSADYEEGYNLRNKRVAVIGAGSSGVQIVAAIQQQVKQLYHWIRSPIWITAGFAQTWAGKKGANFRYSEEQLKFLEENPKKYLEYRKQIENELNQRFKFIIKGSPEARVAREYADSEMRQKLNNSPRLVDKMIPKNFNPGCRRPTPAPGYLEALVADNATIFTDPIGSITETGFVDHEGKEHDVDVIICATGFNTSWLPRFPFIAHGTDLRDLWGPENGVTSYLSVSIPSFPNTFSFCGPYGPLGHGSFMPLIEQWTDYIFQAITKVQLENIKSLTPKMEVSKQFRQHADLFLQRTAWTSPCRSWFKQGKTDGQAAIWPGSRLHFLKIMERPRYEDYNIEYWGENMFEFFGNGFEMREFDGRDITNYLGCLDEDGRDVQPDYDTALIDRLGGFTLSDEYVVRES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR93</accession>
    <name>A0A9W9CR93_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_000520</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR93"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000435">
        <property type="term" value="P:positive regulation of transcription from RNA polymerase II promoter by galactose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF3">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="225"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="494"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="133"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="274"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="334"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="465"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="551"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="691"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="727"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="152"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="286"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="388"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="557"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="594"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="664"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4377691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="919" mass="100622" checksum="B4072098EB82F0AE" modified="2023-11-08" version="1">MQDFPVLSMTNKQDSATLRAAISNLPAHVKYFVEVQGRGDDSNNNSGVQQQQSPLGPSPSTAVAMIILYSITGIITALFLVIIITGAIRAHRHPERYGPRDVLGRPRQSRARGLGRAILETIPIVKFGEKEPAKPTDVELGSTAEARDADGTNADTRAQTTQATAETGATEATATTTAAVTETPETTTKAMAEEHQEGIAPAQVLAVGAASGTSNGNPQDESLGCSICTEDFEKGQDLRVLPCDHKFHPECVDPWLLNVSGTCPLCRVDLRPVTSHDSTASEPNPDDLAPPLDPLAETSHRRRTAFRDIISLRHHPNASAEERISALRRFREQRRNQSGDVVDASADASTEDVAANSRDRRSKRMSVRLSDVFSGRTRRDRRDESPVQGESSTNGAQQPSPPGDQSQTSDANDADNNLRAWLQLPRPRKARMLVPALHAGGVVYESCRHSSHIFGCFPEAQLLPPRPSPDMDTQQQDVPGAQERPAKRTKIRLACQECRDRKIRCDGGKPCNSCMRKKLRPEQCIYSEPSAEIAPSYVKSLESRIRELEQVRGRSESVPQVSMQQDSPDFSGNSAAMQPQKTPETLVPLGMPAPQTTNQYEEMQSPETQTLSSNTFASPPPSNGLHAFGFSQQHPESFERGGLRSLPAPPGSTPKFRAQHHDSGSSGFGNQLRSGHPDHISHSDIAPEDQVASAMGAEGTPPSAKTAGSIFLGPSSAAAFMNLIRKNSQKRKQSDVDGGTPSSTTSRPSRARSSKDRELVRALMEHLVLPPRRVADEYIKSYWTHVHPFYPILHQPTFMEKYNKVWNNHDFEAPDSSETVDSLRFFFSIFNAVLALGCQHSKRTNATLYGNGSANTFFERAQQLITHESPDYASLQLVQSLVLAAQYLQGSDKINRFKQREKNADVRGGVVFSWTESSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR94</accession>
    <name>A0A9W9CR94_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_001474</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR94"/>
    <dbReference type="OrthoDB" id="273452at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000223">
        <property type="entry name" value="Lipase/serine esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF65">
        <property type="entry name" value="ESTERASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12320)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="DUF676" evidence="5">
        <location>
            <begin position="4"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4376193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="454" mass="50785" checksum="2845A91236EA3F13" modified="2023-11-08" version="1">MSGEADHLCVLVHGLWGNPEHLKYLSTALRERFPKDKVHILVAKRNAGSFTYDGVDTGGERVADEVEQKLEELGRNGHDIKKISVIGYSLGGLIARYAIGLLYHKGVFDRIQPVNFTTFATPHLGVRTPLKGYHSHIWNVLGARTLSMSGRQLFVIDRFRDTGRPLLAVLADPKSIFIQGLARFEHRSLYANVVNDRTVTYYTAGISQTDPFVKPEGIKINYIPGYDDVIIDGENPIAPKEPEALPAFTERFASGTRTILGRVPIVAFLAVFIPIGSTLFLMNSLVQSFRSQQRIRLHEEGRAGADFKRYRIPLMINAVRQEAEDMFENINNAQGQDYLSAGSEEVASPTQPSSPGGAAGLRRIRSSHSHFSMDSDINSIEEQKEGLSVEFPTLALTQDQFGMIEALDNVGIKKWPVYIHKHRHSHAAIIVRMDKQSFDEGRVVVKHWLDNFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRN3</accession>
    <name>A0A9W9CRN3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta-lactamase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRN3"/>
    <dbReference type="OrthoDB" id="10250282at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058664">
        <property type="entry name" value="ARB_00930-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051478">
        <property type="entry name" value="Beta-lactamase-like_AB/R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22935:SF97">
        <property type="entry name" value="BETA-LACTAMASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22935">
        <property type="entry name" value="PENICILLIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26335">
        <property type="entry name" value="ARB_00930_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase-related" evidence="1">
        <location>
            <begin position="24"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-lactamase-like ARB-00930-like C-terminal" evidence="2">
        <location>
            <begin position="234"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="394" mass="42921" checksum="7927D8EBD62DDF90" modified="2023-11-08" version="1">MVSPNYQTHRNRHVEHGHFAHEKFFTGLNIMYPSYPAWQTATYSNVAYQLLAYALENITGKKFTDILDDRVIKPLGLNRTYYENAPESVGVIPGTVKDTYWNVSLGDANPGGNMYSSATDLSTLGRAILSSTLIKPSLTRRWLNPVTFSADFLASVGAPWGVRRIQLAKETQPHRTLSVFTKAGTFRKYTAFITLLRDFNLGFTIMMAGNPALNNFLGADLLGASLIPAYDAVARDEADQLYSGTYVSRSSAGFNSTTSMNSSLTISTDPNKPGLGVGPWTSNGVDMLSMAINLQSGSSSAPLRPEARLYYTQLTSNSTDGSGGKRQSWKAVFEDTGGPAAGQQLWSTDCGAWVGVTGITYASLPLDEFIFEFDEAGKVVSVSNLALRETLYKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRN8</accession>
    <name>A0A9W9CRN8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRN8"/>
    <dbReference type="OrthoDB" id="5405126at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="373" mass="41783" checksum="C0D938A3E8A2E0F5" modified="2023-11-08" version="1">MAQAAPSPSPAPAREVRTLPVFLEPSSPELSTLLSTLNSKVLLPHHLTPAQQKLVYKAENRAKLDAEPVEISLGDVTLPLEHLDRNHLPNRWKHLREIVRQSSTREDWENVIRVLEGFENAGVKVKSEWRELVVRNLNASGNQHLVLKALQRAKATGLRLNEYGVLVQVCKGVHDRASLAGWEVEETAKALRFAKQIVELMDEEEHCGGQARGEMVSERDWRSRPFVIALPTELAARLALSENGGGNVEEVAKLAKRLVVALQQSQFTTDLETTAHLTSNPPPFPNGTSQIQFITTHCYSLLELVIVWNALKTSRKVLGANMPMKDEAAEIESRVQKVLEEGVEKVDKLRVRDGKEMRNAYVGYVRDAVERCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY24</accession>
    <name>A0A9W8XY24_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_010011</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY24"/>
    <dbReference type="OrthoDB" id="3945418at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF157">
        <property type="entry name" value="N-ACETYLTRYPTOPHAN 6-HYDROXYLASE IVOC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4362894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="391" mass="43663" checksum="1A70EEA0CF4924B7" modified="2023-11-08" version="1">MQTHEGWATLLLSAALATVIAYTVYTVIYNVYLHPLAKFPGPPFARLTIYWKAYVECIANRSFCHLHFAKPNAYHAIYNNKNRWDKEARLYKSFNEDRSSFGFITYAEAKNRKDVLGRSFSPAAIEGAESLCVERTKALCAAFERQSQAARSADMLYAFRCMTMDIITAFSFGKTVDAIDAPDFKAPILLAMDASMPVFIRFKYSDLYKNMILKCPPKLSKILSPSTAGLVDLQQLLLRQINDLTEDPAKLKSLPHSMTIYHRLLDAEAYRNKTVPSAGSLYEEAQALMFAGADTVGNTLMLGTYHLLQNPAQLQKLKKELLAVWPSLHAHEPKLRDFETLPYLNAVIKESLRLSSGVVSGLLRVVPSTGAVIEDVTVPPGVRTSQITNPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY80</accession>
    <name>A0A9W8XY80_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_010969</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY80"/>
    <dbReference type="OrthoDB" id="2152896at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="164"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="321"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="90"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="321"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="347"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4362028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="383" mass="40829" checksum="531C84AAA1035B55" modified="2023-11-08" version="1">MAAAAKGITGAPLEGSTSGGRRPSKPSSQFRGPSASLNSTMASSMPGNGSGFAPSSYGAAAAKSEAVTDGPALASMPEHEQGNSKSRIRRASEGSRLSKGDSKRASASDLRCEKCGKGYKHSSCLTKHLWEHTPEWQYTSKLLISKHQQVQLLEAASVLVAMNKESDGGDSDRSSPPASGSSDLRDELSSTETTPPPQSEDHAVGSYPLSAFASRSIKRYSTNSSAYSHSYQSAVFSENGNGGHFRHWSNVSDRPTTSGTSVAGSYHDDGNDHADLAAAVGLLSCSYGTPKSGPVALPPDVPPVPPLPAKFMDFNNARTLSGSTTVTAQQSSMRNSFRYRNESKDVDMDDDHFADEEDYRHSNSRSRGRIEEDDDAFFGRMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYE4</accession>
    <name>A0A9W8XYE4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010376</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYE4"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF8">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="8"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="179"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="272" mass="30827" checksum="1F0CD923FFEC7617" modified="2023-11-08" version="1">MEFKCSSGGWVLGGLNSEAAWCRHMCNKANIKVIDVDYRMAPEYPFPTSIFDCWDAVKWAIYNAQNLNIDPGSVSIGGLSAGGQMSAVLAHFARDEGINLKLHMMIVPATDMRYCLKGLKLDNSNCPYQSVHLFHDVPWGPLGREQWFLKYWLGDSVGMLPITLDHCIIRADGLIEEQEKALKNWICTPVLAPSFKNLAPAHIITAEFDLERDEGEYYGELLRKAGNRVTMKRYAGVPHAFGHYNHPERGLKKSFEYIEDTSRLLRSVHYDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYG7</accession>
    <name>A0A9W8XYG7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">methylated diphthine methylhydrolase</fullName>
            <ecNumber evidence="6">3.1.1.97</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010406</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>diphthine methyl ester-[translation elongation factor 2] + H2O = diphthine-[translation elongation factor 2] + methanol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42656"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="EC" id="3.1.1.97"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DPH7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.97" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYG7"/>
    <dbReference type="OrthoDB" id="1930760at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061685">
        <property type="term" value="F:diphthine methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052415">
        <property type="entry name" value="Diphthine_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46042">
        <property type="entry name" value="DIPHTHINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46042:SF1">
        <property type="entry name" value="DIPHTHINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4362313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="377" mass="41705" checksum="97120FD9BAD989BC" modified="2023-11-08" version="1">MTSIQSLVSFKLDLPPSCIEFFPLNPQYAVVGTYNLQQQGEQEPSDGAHVAQSKSQQRNGSLILIRVVGDNVEILQTLSTPSAILDIHFLTQVSSSNFGVATSTGTFGIYALEPWQRDPKLVHIKTIQYFSEDVLITAFSWHPESFMVGMTLSSGQVCLGVIETEEETAAPLHMDVVRHDLEAWTLAFLPDGSGLLSGGDDSALRFTELADGSATSMPWADKKIHGAGVTAILPIHLSNEGSLIVTGSYDDHLRLIHVSTSGRRQLHAETNLGGGVWRLKLLERKPALPAHHGVEKWRSEPPPEDVLLLVSCMHAGSRIVRLSRSATSDWKIEVLAKFEEHTSMNYGSDSQPDMNEKGQRTFITTSFYDRLLCFWRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYY9</accession>
    <name>A0A9W8XYY9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Component of a membrane-bound complex containing the Tor2p kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">AVO1</name>
        <name evidence="5" type="ORF">N0V83_009675</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYY9"/>
    <dbReference type="OrthoDB" id="241990at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031567">
        <property type="entry name" value="CRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008828">
        <property type="entry name" value="Sin1/Avo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031313">
        <property type="entry name" value="Sin1_PH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13335">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT MAPKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13335:SF1">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT MAPKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16978">
        <property type="entry name" value="CRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16979">
        <property type="entry name" value="SIN1_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIM" evidence="3">
        <location>
            <begin position="344"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="SIN1-type PH" evidence="4">
        <location>
            <begin position="727"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="79"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="455"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="513"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="212"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="272"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="519"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4363382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="847" mass="92609" checksum="2C696764933B52C5" modified="2023-11-08" version="1">MSLLQNEDFVLYQLRTAYLSAIKDGVGERLINLDSGVLNNPAFRAAGWVPNAADIKRTYSPPIPTAITSEYFQAPRSAGLKAPEGFGDDEEEGGMVTRGGSNDTVGPTLNAKRRRRKEQLDEDDSSDLSDESDDDDDMNRPANQIKFTKMPLRTRSGSSPIRGSALRNELEDAEGGPSVMVTSPSRPPDSSLRRGSFGTLDAAKQRPRRDTITSSEMSSENEFDNSTFRKRRVHARKAAGASQLLSQRIQEDEGEAAGARLEALHEGDESDSSLSSEFSGSADSGSLIGAGGDPLDSSPVAELPTIPGLTPFNNLSPKKKRELPQPTLQALPPPRPISFVLPVSALTQALQAKEQKPSNPLQRFARWNAASEAQQNPLYIRLYCPFSSKPSKPMELLIRKFNDKGEGPNVAEAIGYALHRYQEEKLEPPLTPEKMNVNGWVMRMYDDGEVDDEFPPLTRNKPLKDFASNNARGMRPRAREKPWDEFALVEATQREMVENEKITPIYTQEAAAAVASQKDASEEQEEKKPLKPLPNRAKSFRNPITGPSFAPTATRKDTSTLADVPKVSQSHAASRTGAPRTVTVHFTDENFNTKHIPVSCTTDTYIADIFDQVCRDLRLEKALYILKVSGSTTVAPPDRTVEALDTRMDLDLLRRRFVGDGVFGIAGSPGSTSPNAPLIISTGGAPKKTKKGERTHAAVSGHALVDPLTKHSGHSAGLGLTLSTTGYYRRYAVLRKQPMSFASTSSRILALDNEYIHIMPGESGANKAAGGIGGLGADGGQGKTTTVHFSSVVGSKVSKKHPKMFRMFVFKERETKRYDFQAGSHDEAVEIVREIKRGVERFKEGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ37</accession>
    <name>A0A9W8XZ37_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dienelactone hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010941</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ37"/>
    <dbReference type="OrthoDB" id="17560at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17630">
        <property type="entry name" value="DIENELACTONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17630:SF105">
        <property type="entry name" value="DIENELACTONE HYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G08790)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dienelactone hydrolase" evidence="1">
        <location>
            <begin position="29"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="292" mass="32019" checksum="3939FC1CCED20F7E" modified="2023-11-08" version="1">MSCPDCFRGSAHTNQGEPSGQVLPVHDIQCYVASPPSTTTSRSTIIFLPDAFGHGFLNNQILADKYASETGFRVLIPDVIRGGACDPKALLYMDAVSEPVAWWDIVGQLKRVLTVFKLVSIFIPFLIRASPYKTYPKILNFTRSVKAELPSGAKLGVCGFCWGGLPSTMLCAEPATPGSNERLVDAHFTGHPSRLDAPKQVIEAVEKFKVPYSLAIGETDIAMPRKQVERTEAELRTKVGRGEGENGFFYEIVNYPGCGHGFAVRAKAGDKVAEKGAEEAYEQAVTWFKRWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0A9</accession>
    <name>A0A9W8Y0A9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_010576</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NUP186/NUP192/NUP205 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2019644at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021827">
        <property type="entry name" value="Nup186/Nup192/Nup205"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11894">
        <property type="entry name" value="Nup192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4362482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1665" mass="187817" checksum="3B8FC17DB6A47650" modified="2023-11-08" version="1">MAEDDSLAALQGLHRDLCAHIDLQLPVLERLLQNLEVHLDELKTLLDKKPKNDASRQALSSGKIKIGDDEYEVNDDFKQQTLELADALDLDELDAAALFLGAEDEAQELDRSQLQTAVIRFHRRREYVLLCLRILMKTALDNGESEVAGMQALQMAVQIIVGVEGTSNLANAYGFWTRCLISMESIEKWLQFIAERVQSTQVVGQVPLPGFVEIMDFQRQSLTRQHENLAAICTHMIKAGYVNLDNYKALLARAKTLDRHDLITVHYVPVLIRLTSWAASETNVTLKDARALHDSLMAGRESDNWTLRNLHSALLVWWLAEYSGRYVDPNTQDPAMQNINFDEEAIARSDIFLKALNDGAFHFMLSFSQDVRPTRWYDPQKTSMLSTLLQDTPVLGPESAQPEEFFKVLIMEQLQTFVDSFITHMPDTLRRLKVEEDDQRRRLQLHFQRSTGEYPLHLERFMVIVAFAFDGFPDAAEDFWTDKESNLYGFLQWAAKRQPTPRIAMFCEMLRAISTGGSNPDSAHRFLLEEGTSSSGKIRRTSSLSYTHIFNELKEFEKDIHEPKKTLGNVYAPSTNPVDQIVEPESATMLESYLRLLAHICRQSLTARTWILDLKEYDFANICFGLCTDKVESGLRAAAYDALSALLVGKDVLRASAMWHILDDWTVNGFYAGSKQSNTLGAVPRDDFWALLADGYDESIALVRFLHSLVEPYPENEGLNDSLPFPESLGSSRRMPGIDSYIDFVMHQVFAERSQEVLEPLQRNVMRWTCLQFMVTCLSTFNENLVVFANKSSIPVDAVIDSSSLATYVALHPFTRVMEWLFNDKVLKALFAASHHDVNEVDAAPSDSPLVQSLMLSIEVMDLVMKLQATYLDIVKPVLKQQPSMQNSPVSNLSLASFEDAILNNLHIIVDLGLYCGTGHEALTIASLQLLEKMASSRKLAVSPAGFGQRSGRSKIVGILEKDNEAERIGRSLSSLMRFNAEELEAGEEASGYIVKLRVLDFLNSCLAAVSLRPTIAHILLGFSCGTNSVRIADDSLWANGQALFHNILLLGVEYPDNDGEQFLAWRSALKTRCWDVLQKLWRSPLTSAVVMDELRGNELLFIEAPRLSPITLDVPWEGVTLAQGLETATGEFSEFFAGPPALALQNVLQRRAAFLDYESRELRLTVKEDMPTMRSRIQSVLLGTTIRPGEDATPNLNIFDLFDFIEFTYPERTLPDQHRFFEGVNLSSCQVEKEGITLYSLPLLEQVIRLKLKYWRKDGLITELAQEEELLQQAELLMAIFVDWNRRAQLLRYHEEILQSWVQVVMVAIHKCDFENGARSSFILQTLQVILPKLEQSYNTDIFTAMQLISLAESLVQKIDFESTAFDKTGDFANDRLSQLFRAALTGIYSAVSTAELREYCYQICFRYIHGTFNKANTNSLLSRHTLNAVKNCGSHLMEVVCDDAYSGQRTCKVSALLLLNALVAVATRQQSKYILETFVSLNFVGVLVDNIKHIPEELRAAAAPQVPVLLSYYDASLALLLRICQSRLGAAYVLNAGLFPAIRDSNIFSIDPDIGLEFDNPNALKKYYELMLAVLRVVNAAVIARGRQNDQTVFQAREFLKENRHSMVSIFKRSVNVGAGQYEEAQQDLVDLVDCWTVLVDVTGFLQFEDSSSLKKARPNMFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0I6</accession>
    <name>A0A9W8Y0I6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="8">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_009250</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0I6"/>
    <dbReference type="OrthoDB" id="3264224at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF20">
        <property type="entry name" value="[3-METHYL-2-OXOBUTANOATE DEHYDROGENASE [LIPOAMIDE]] KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="9">
        <location>
            <begin position="270"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4364653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="481" mass="52978" checksum="021E01CAE8B7D877" modified="2023-11-08" version="1">MAQVQRPLVIQSHIIRACCPRPARRTTRALFARGNHHHKLHTKTVTDNDISRLASLPLHPLTLADLVKHGRPPLTTQQLLTSANFTLSILPARLAHRIQSLRNLPFIVVSNPHVSKIHSNYIHSLSTLLPWAEQEITTLEEEVKFTEVMADLVQTHANTISILARGFLEARKYISPKDVTRFLDEHLRARIGTRLIAEQHLSLHFSSQPHSEVMHEQDDNPGYIGVIDTKLKPARIVDHCANVVGEICELKYGVRPTVVINGEPEYEFAHIPVHLEYIITELLKNAFRATVESGMERESVEVTIAPLPELLPESTGSDADTISKEKVENEDQGNMDVASQRFTGHTTTTSPTADANILPLKHSTPGVTIRIRDRGGGISPENLAHIWDYSFTTFNDDQASSTLTGSHSGNGMDALNAFSGPGGDGANSLAGLGYGLPLGRAYAEYFGGGIAVQSLWGWGTDVYLSLRGVGRVEGEENGRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0M0</accession>
    <name>A0A9W8Y0M0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_009282</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0M0"/>
    <dbReference type="OrthoDB" id="69177at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000020">
        <property type="entry name" value="Unplaced genomic scaffold supercont2.4, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF247">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="7">
        <location>
            <begin position="352"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4364685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="481" mass="52795" checksum="99AEDE1D2208D7E5" modified="2023-11-08" version="1">MAKRKRNTAHTNAVKKQTTSLPSPPQEANPLEPSSVHSVISSEEIEVAVETLQTLSEHPNLIKSKACKDLRTAVYGFRQACTTGLNSAEGSNLTARISAALTDAKYMDALILLAEMRIRKDTPTLGALCRWVRTLDVVSGLSIHVQVPSQTIVGENAQNSELIQVLDAILRVTGPTDTTPHGTDAASGPISLQKTWNLRDTNEPTRQVRRSIEDKSIFASCSPNLKEQFRILETTPGLQRKPPNLHPAILYASQDDTVLLAQGPKTSCHKHPIVPDLRLIKDVLSPAECSSIIAAGETIGFIPDAPMRPQGEDTSILAHNFYWIVDQAFHDKLWERVKPFVPEEVAGKKVRGINRRFRVYRYVPGAEYRCHIDGAWPPSGIHPSTSTYQYDASPPSARQSSLFTFLIYLNDDFTGGETTFFIPSVKDGVINAYPVNPIMGSVAVFPHGEARGALLHEGTGVVEGAKYVIRTDVEYDVDATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0R8</accession>
    <name>A0A9W8Y0R8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein bL32m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_008616</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0R8"/>
    <dbReference type="OrthoDB" id="2014905at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051991">
        <property type="entry name" value="Mitoribosomal_protein_bL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002677">
        <property type="entry name" value="Ribosomal_bL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01031">
        <property type="entry name" value="rpmF_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21026">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21026:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL32M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01783">
        <property type="entry name" value="Ribosomal_L32p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="137"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="137"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4365000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="178" mass="20164" checksum="D90F6C0176D3AD24" modified="2023-11-08" version="1">MALARPLPPLWQTLFPALSGPVRPVVNLPLLQRLSQPFGALTTPFGALAIPSLALPKIPSLAEIWDGILNAVPKKKTSYRKKRQRFMAGKGLKDITSLNKCSACGRVKRLHILCPYCVDAIKTNIFGQINWSIRRPTDKQVKQMARERRGDTKTRLTHQPDPRKEKEDQILKHTGWKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0U6</accession>
    <name>A0A9W8Y0U6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009572</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0U6"/>
    <dbReference type="OrthoDB" id="3795043at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="417"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="522"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="592"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="627"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="707"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="855"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="251"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="383"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="421"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="527"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="602"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="635"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="717"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="787"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="875"/>
            <end position="889"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="954" mass="100756" checksum="8F1CFCE3B231B525" modified="2023-11-08" version="1">MSTLHTSAAGAPFAQALTATDRDTEFTQQATLIPRSAMDHGVQPNQANSNVGNMSTQLDTSDHGSLTIESSLDTQSSLSHDSKVTGLVARYESYAGSSDDLEVPANEHATVSSDTIRIPSSYDLENTSVPSEEHHKTITRTTGHTVQGQFSPRKPIPFQWLAKSSGPNLATAQRAKERSHKKDPHTTSPMEMATVRGTRSSVDLGRANSSEGSTFLTKEALVAVESNIAAATSPRSPKTLHRSPSKPAWNSPSVSPLRSSLRQQSGLTVAVSPTKPTFLNAELTSSGHARGYSSAATSFHTAEGSSVRSSTGSELSFRTAEEILEDVDVPHLNLNADSETVQTVRIPGSSTAKGKDDARAKGVKPKLALNIPPAGPLSITDRASAAASTSASGSSDKNTSPLSPIFRVQTSRIPRIAAASKTGSTESATQGSMLEQAQPVKSLTSKAKSKLEESLETLEGYPANPSGTALTLARGSEVHSVTSMCQKAVQTDLPLDPAQHYPNEKVEPTLITTTMAERATAGDDFGSSVSSRATSSTTGKASAALKDPVLIDPAIIYNRKKPYTLGTMLDRPITMPPIIVMMAHTLDIGNMRLNDPRDDNDSSLSSEATATIDASSQIPGFNESYVADSKKKAASENSKQSSSNTDLRATAPEFVPQVPQQTGSSGLPLSNAELLALPLPDMNELDQYGVPWLYYMYPVHAAYQQGFHNGRSTSPPKRFKPKRQRGPRSTPTAGQLVEPASASTPKETQPAAAPPSEVPHRPSSAELMPPPPVPTKPSQGPAKHENSQPGVMTQQDPGANTHPNVIIVDNPFASQLDIINQQAALSNTTDTNAPRSPNVDLTSIRNVLPASGPHTAYDRASHQSLPYRGPRQGNRRGGNGLYGGSGIAGGVPMDATAPFPDPIAPRGRRAGGRGQVGPPVQYITRTIGTEACGIIEIEAAMEQIGGMPCHACQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0X0</accession>
    <name>A0A9W8Y0X0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein bS6m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010578</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bS6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0X0"/>
    <dbReference type="OrthoDB" id="10259681at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15465">
        <property type="entry name" value="bS6_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.60:FF:000007">
        <property type="entry name" value="37S ribosomal protein Mrp17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000529">
        <property type="entry name" value="Ribosomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035980">
        <property type="entry name" value="Ribosomal_bS6_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00166">
        <property type="entry name" value="S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS6M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01250">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54995">
        <property type="entry name" value="Ribosomal protein S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4362484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="145" mass="16493" checksum="6B12774DC4E3D9C6" modified="2023-11-08" version="1">MGSRVAERLAEFEYFSRRTADTDKLQHHGKPGKMLYEMIGVVRPGRLSEVKEIAKTAGKIVLDQQGVVRGVSNWGTFLLPKPAKKLQSTHHYGHHFIMRFDASARAQHALRRTMSLDPRLIRYSVVKMGTKFEEIKDVPGTASFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y125</accession>
    <name>A0A9W8Y125_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008637</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y125"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="91" mass="9629" checksum="7CA4512B2A01DCF4" modified="2023-11-08" version="1">MSSSASATAAPPLPPPPFFGGLSQPRVGFSSGVSEGQALADAFYRLTDDAQTVVAGFANQDLLGIYRLTDEEQTAVVRFVAEELRPRGLWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y141</accession>
    <name>A0A9W8Y141_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_010683</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y141"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF237">
        <property type="entry name" value="CYTOCHROME P450 MONOOXYGENASE ATNE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4362589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="412" mass="45789" checksum="A5E5BA3635769426" modified="2023-11-08" version="1">MFVFNAASNELHSKRRKIISQGFSEQAMRASEPKILKHVANYCNVLFEPVTKTGWSEARETTQWSAFFAIDLIADLTVGRSTAMLTSPENRIYNPARKANFARMGLAIQWPEAFRGGLFKNLKLGELLFPKIAKLGKQWQNLLSSWIAARLKELRSVEKLATEDDMIVAIARYKDPETGQGLSDGDLTAEMTTLLIAGSGTITTGMTSLLWYLSRWPDSYGKAAQEVRSIFKTPESIGMNSTLSTCIYLKACLQEAMRVSPAPTTPLYREAGPGGAVVCGIHIPEGYEVATTIYALHHNESHHPDSFTFDPERWLQDKENLRATKAAWAPFSVGDRNCVGMTLATNEVLIAMATILWYGDFRTADDPSLASIGAGSQRLGPGRQRVKEFQLYDTFGASTEGPYLQFKRRKIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1G8</accession>
    <name>A0A9W8Y1G8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008953</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1G8"/>
    <dbReference type="OrthoDB" id="7295497at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051061">
        <property type="entry name" value="Zinc_finger_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179:SF19">
        <property type="entry name" value="C2H2 FINGER DOMAIN TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="597"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="646"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="195"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="365"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="449"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="560"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="195"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="365"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="391"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="466"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="477"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="565"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="720" mass="79708" checksum="D768E693C5871ECA" modified="2023-11-08" version="1">MLALPTRRQHLPQQHHTPTIPSPPSSQHLSYQQPQQFATQHLFDQKLLHDYLESQQQSFGTSAVSAPNSFMTSTYDFANPAIRIQQSTPTPQLPQSGFSQPPMLSTNVANTGLESWNSYGNVQGQSLQTPVQNQNRGHQRASSSSSVGSSVSQYQAVGPSASYQYVARSEYSSPHSATVDSSDMADTLLRTFSNHLPTPTHTPTQESFMNTNNYNNYTPTSSTMDQTMAAHMSMKQALMDQGDDVPDFAHSARHSVSSYGHDSPATPHTATGEDMDFKMPANGETPHKVDAWQFNQFCTYDDDLEMRQHTVPKFERTYTDIAADYSYDPSAVAASSIPQTKPAQSSLLSPYRSNTNENVQRALQAAQYARSQSPSSSASRGDSPFRRGSPYRQTNSSFNSPMVGTAAATREQNRQADAAYAMKPQMQSEEAKTISPKDALLDYREADEDSKAPLFPDGGASEYDGQYNSGDQYRNATQSSFDTTSQQSYRRDSWATPQFSSNFQTTATPSLPQQTSNFNFATPTVQGNMHNMPLASTSQYRPTSNNMSSAVDQTPEFPAHLTSMESSASEAEPPNGSQGSDLFLRKPTSSAADSGTYSCTYHGCSQRFETPQKLQKHKREGHRNANLSTAMTSAAILERNSQAGPHKCERINPTTGKPCNTVFSRPYDLTRHEDTIHNGRKQKVRCALCVEEKTFSRNDALTRHMVRMHHQTRKHPMQPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1H6</accession>
    <name>A0A9W8Y1H6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1H6"/>
    <dbReference type="OrthoDB" id="5333304at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="717" mass="81576" checksum="8C97A56C87462AD4" modified="2023-11-08" version="1">MSTAAIPSPLANGASVVPAEVLREADFLQKLLQIRDEVLASKHPRIHLPAKVIEQVAPQFPHATSKPTTNGTTNGLSQLLPPRPESSLKHSTSPIPPIQRPYPTTSSSSIDPVLLTKSDHLIRAELQLKRQQIERLLKDQFDRKGRGNDAEEREANINVEECLAQAHLRVPPISGLPSTTNNSDGAESFDENSYYSSKADSWSPDEFDSNQNNKADAAGALTSQTTQAVKPVATQVQQAEPTVIDLDEEAYEPADDIEIYEPEEAEEEDYSPPPADIRPNEPFRGRARDRDNANRSGTNGHARRQSPNGPAAPLNNPRKRRREEKQVEKRRQQQATKRAIRSPEPYIKEEPQSPPPVSVYPDSQPSKRRALQPIANDEEVMSPRESARMQPVYYQDLEPSSRPLREYVEPASPSVTRVPQRKVHRDDHDLRRVASLQYARRPYSPNDGGGEVYAVPEPRQLRAASHAFVDRPEPPIYREASIRPSAAPRYVRERSRSAGHEYLSRQQSPIVMAPPPRRIVVDQYGNKYYAAPVDARESMAPPSRRVELDPYYERAVTREPTIRAPAHTDLYEEDTVHRMPPPPRRYVEASDVEMIERPVYRQREASRRPVEVQYRPVPQYDEMGPPREYAPTRAYSMRPEVVRREIPEGYVRHESIQPGPVRVSQPRYREVSVVHQEPVDDRRYISAPQTRRYVEEGPIEVAQEPYAAEPRRVYTRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2N5</accession>
    <name>A0A9W8Y2N5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Carboxypeptidase</fullName>
            <ecNumber evidence="15">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">KEX1</name>
        <name evidence="18" type="ORF">N0V83_008761</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Protease with a carboxypeptidase B-like function involved in the C-terminal processing of the lysine and arginine residues from protein precursors. Promotes cell fusion and is involved in the programmed cell death.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential release of a C-terminal arginine or lysine residue.</text>
            <dbReference type="EC" id="3.4.16.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="15"/>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2N5"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000121">
        <property type="entry name" value="Carboxypeptidase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF190">
        <property type="entry name" value="PHEROMONE-PROCESSING CARBOXYPEPTIDASE KEX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4 15 18" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 15 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 15 18" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0732">Signal</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041016374" description="Carboxypeptidase" evidence="15">
        <location>
            <begin position="31"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="519"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="472"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="577"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="577"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="628"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4365143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="638" mass="71818" checksum="F06BB8EA3B20CAEA" modified="2023-11-08" version="1">MAFTSSASRWGTAIIGGLLATLSWTPSVVAEKTQADYFIHSLPGAPQPLLKMHAGHIEVDAEHNGNLFFWHYENRHIADKQRTVLWLNGGPGCSSMDGAMMEVGPYRVREGGNLEYNNGSWDEFANLLFIDQPVGTGFSYVNTDSYLTDLNQMADHMMIFLEKWFALFPEYEHDDLYIAGESYAGQHIPYIARAILNRNKDQTKKPWALKGLLIGNGWMSPVDQYLAYIPFAYQNGLMQADTDMARRVENQQKICINKLNEGGMDHVDTSECEQIMVAILEETKDRKADRMNQCLNMYDIRLRDDSSCGMNWPPDLTEVTPYLRRPDVIQALHINSDKKTGWQECNGAVSGHFRAKNSEPAVKFLPELIEQIPVLLFSGDKDFICNHVGTEAMIENLRWNGGKGFEVSPGVLTAKQDWMFEGEAAGTWQEARNLTYVVFYNSSHMVPFDYPRRTRDMLDRFMGVDIGAIGGQPADSQIDGEKGPITSVGGHPNSTKAEEDKTKQLKEAEWKAYYRSGEVALVVVVIVAGLWSLFLWRNRRSASGYKGVDGDEGRESLLTGMGLDNFRRKERRQDLEAADFDERELDDLDEGPKKSANGYSNGRSSEKDRPHNDSTFSLGGDSDDEAEGSQRGRRKEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3L9</accession>
    <name>A0A9W8Y3L9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription factor TFIIIB component B'' Myb domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007429</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3L9"/>
    <dbReference type="OrthoDB" id="272624at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001156">
        <property type="term" value="F:TFIIIC-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070898">
        <property type="term" value="P:RNA polymerase III preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039467">
        <property type="entry name" value="TFIIIB_B''_Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22929">
        <property type="entry name" value="RNA POLYMERASE III TRANSCRIPTION INITIATION FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22929:SF0">
        <property type="entry name" value="TRANSCRIPTION FACTOR TFIIIB COMPONENT B'' HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15963">
        <property type="entry name" value="Myb_DNA-bind_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor TFIIIB component B'' Myb" evidence="2">
        <location>
            <begin position="608"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="729"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="260"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="781" mass="85282" checksum="83C1036203019309" modified="2023-11-08" version="1">MSAEDGAPKPPAAGSDKAPASKAATTFSSFINKNTTGKKFAPKAARRRPGATPAASAPEPEPAPVVDSQPATAAQLPTPATTQEPTSPSTTPAELGQVASATTSAVASHAPEPATDALEPLPEAAAASISEQPQDGQEPQIDGQISSPGRGTVRAAEDEPELRRAPKRRRVDQPAPKDTSATSEPTTNAENTASSTESARQDMGLDAPQQPNAPQQFDAPQQSEAPQQPDASQQPEASQQPEANVPITVGAPAAAPESQVLVAETHAEAVAETAVVQPSGEDVTAPRPRRRGRLPWVAVNHPRDDEDGAVSGEPTRTTRGTTRSRGKKKAMADDVIGEANDEEGIQEGTEEGEPRPVRKRPSAKARGKRKATSTEGSAETQPPRKRSRRTRANNETEDAGLVDADDQEAEPEADGEAEVVTRRKPRQPKRKKRQSTEGTAEGDAESAQPKRRGRPPREPTPSDAEDQTIDPETTFMDGLASRNIRFGKLSNREKQMREIDWEAVKQRRREEDARPIRSKDEQRLIDGAFNEAALNAPQEEGPRMHLVDGVIQMMPNTGTVDREAAADREIAEYEIIEDRDITSRITSRSFMKNNKRFPNDFILPGQGKRWNRDSTDLFYQGLKSFGTDFQMISHMFPGSTRRSIKTKFTREERDDPERVREALQGTSEIATHWDVFLEASQRREDSFVDADDIKRQLAEEEELMREKIAAALEETRQRNLQKAAAGVLDDNEDLEAENGNSNKENGKGKKKRRETAKQVTFQEEAGVEIVGSLDDDPTWGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y497</accession>
    <name>A0A9W8Y497_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_007806</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y497"/>
    <dbReference type="OrthoDB" id="629492at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904:SF523">
        <property type="entry name" value="STRESS-INDUCED-PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904">
        <property type="entry name" value="TPR REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="7"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="4">
        <location>
            <begin position="131"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4366171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="613" mass="70158" checksum="19C490A6366DA866" modified="2023-11-08" version="1">MARRTSPEEYQELGRRYYKLKQYEQAIDVLTQGIEVAPTLGLYDHRAASYDKLKDFNAAVKDGREMIKLDKKEVKGYLRTASVLEKMEKPDTALGIYRYGMKNVSIDDKNFKLLQQLHDKLTRKLSPATAIDPFTILPVELAEMILEYLSFKNMVNCMRVSKGWRDYLAKRPKLWLHLDMSGARKPVPRSFVGNAVRRSESRLKRITVHRFEHMDVLKNIAKTCKGLTDIEFVTLPHTVSSTLIDIVQCSPSLKRFVVHPEISTDTVSQILHYQSSLEHVRFSGVTSSQIRPDWKGPPLEALNTLSIHMVEIQSAQKLKIEHLMRLASNLQSLTLSNMKNLTVPTGWPDEIDHLPPLIHLNLKRIQFSHRLVLFPPTLQRLEVETDGVYKCRIHDFARQPFLPRLTHLKLWGLEMLSAGIFEDLLDTPIDAMDADEETVMGATPLQSLSVHCLLEREDQDNGLFNGPDSLFGRSRRILTPALTSLDIATLRCDDDEIEHLLSQYETGIQSINLCCTNITGASIKMLADKLPWLKTIKADNCHKINGRDAIYYAERKGISDARKLESLNAMQCFRDASPNEEPIEPSQIDRFPRVDVEVLLERKDRVTYPNEMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4B2</accession>
    <name>A0A9W8Y4B2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lysyl-tRNA synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MSK1</name>
        <name evidence="7" type="ORF">N0V83_008037</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4B2"/>
    <dbReference type="OrthoDB" id="21243at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070154">
        <property type="term" value="P:mitochondrial lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04322">
        <property type="entry name" value="LysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000094">
        <property type="entry name" value="Lysine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044136">
        <property type="entry name" value="Lys-tRNA-ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018149">
        <property type="entry name" value="Lys-tRNA-synth_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918:SF5">
        <property type="entry name" value="LYSINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918">
        <property type="entry name" value="LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00982">
        <property type="entry name" value="TRNASYNTHLYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="6">
        <location>
            <begin position="221"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4366401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="555" mass="62812" checksum="B805A19D112E9230" modified="2023-11-08" version="1">MSRTSLSFLRPYLYRASAPAKPEYARAYVSIQRRLAQTVAAENFTGPHDVEKQKRLEQLKRVKPLGDYHPRLVYSTGAESLPLRDFHAKYESIQETNPDVVSVFGRVRSVRLLGSKLMFLDIERDTQRLQVMVELKKLTARDRLEENFKSFKKVARIGDWVSIKGNPMRTSTGQLSILAAEVPQVLAPCLHHLPEKVDDAETLARRPHIHSLTSDEPGDILRLRALIYDYIRTYFIQSGFLEVETPFFDVNAGGAIARPFETVANELSNMSIRLRIAPELNLKRLIVGGQDKIFEIGRSFRNEGVDNSHNPEFTTCEFYEVGATLPSVIARTEQLLHGLHTAIESLRSTYFPSLTAPEGVDFSAPFAQLPFIPTIEQASGRKLPDLSSPLASEEVLKMFKDLNIDIPPNSTLPRLLDTLAEHYIEPLCHNPTFITHHPEALSPLSKSFVDPTTNQRVASRVELFINSREYVNAYEEENSPFEQRRKFMQQLEFHPEGEAAIDESYLEALEWGMPPTGGWGCGLDRLVMLFASKKRIADVLPFGTLRNVVSIAKHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4S7</accession>
    <name>A0A9W8Y4S7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Major allergen Alt a 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ALTA1</name>
        <name evidence="8" type="ORF">N0V83_006399</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4S7"/>
    <dbReference type="OrthoDB" id="3928926at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd12798">
        <property type="entry name" value="Alt_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.350.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032382">
        <property type="entry name" value="AltA1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16541">
        <property type="entry name" value="AltA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51895">
        <property type="entry name" value="AA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040881732" evidence="6">
        <location>
            <begin position="21"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="AA1-like" evidence="7">
        <location>
            <begin position="37"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="76"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="130"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="160" mass="17305" checksum="A6112954FFA1F8FF" modified="2023-11-08" version="1">MHFTLPTISILAATLGLSAAAPLEARQDTTTCPVVETGDYIWKLDSFYARKPDGKTINSLGFNVKATNGGTADFNCSVSADVVEDSKFYQCGENSFIYFAWQADRNGLLIRQDVSDTIQYIGTATLPDYCRAGGNGPNDFVCQGVSPAYITLVQSPVKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y560</accession>
    <name>A0A9W8Y560_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_007702</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y560"/>
    <dbReference type="OrthoDB" id="443402at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4367172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="147" mass="16503" checksum="558E64D43FB04D1E" modified="2023-11-08" version="1">MSYHAILENATKNLSELHNDLTREAPIKQKLSVADKQLVALQKESAAVAKKLRAALEKAKVKNGHRKWRSVYQAIRSVYSEQEISKLATQLDDIRKQVDTVLLVSLRYALSDIVFYICHAKLLFKTGGRSSISIPNGATKNEPDISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5B7</accession>
    <name>A0A9W8Y5B7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribose-5-phosphate isomerase</fullName>
            <ecNumber evidence="4">5.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">D-ribose-5-phosphate ketol-isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Phosphoriboisomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RKI1</name>
        <name evidence="9" type="ORF">N0V83_006582</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5B7"/>
    <dbReference type="OrthoDB" id="1555531at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006014">
        <property type="term" value="P:D-ribose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000014">
        <property type="entry name" value="Ribose 5-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934:SF0">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75445">
        <property type="entry name" value="D-ribose-5-phosphate isomerase (RpiA), lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4368226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="284" mass="30125" checksum="31F8C4B1BA4F38CA" modified="2023-11-08" version="1">MTDTNVEAAKRAAAQQAVADHFDPSASFVGIGSGTTIVYVVEAIKERSTNPSVLFVPTGYQSRQLVINAGLTPISFDSIPDRVMLDVAFDGADEVDTELNCIKGGGACLFQEKLVAERAKKFVCVADYRKRQDRLLTNWPTIPIEVAPLSQHKVEASLRLLGSTNPKLRTTLLEKSGPLKTDQHFFIIDAPFPALLTSADVAAGKGKGDGSDGTWEVNNISKAIKGLTGVLEVGIFSGMNGNDAQALRAKDGALSAVTGGQKPIAVYFGMADGSVKLRTADGEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5G8</accession>
    <name>A0A9W8Y5G8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_007150</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5G8"/>
    <dbReference type="OrthoDB" id="29596at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051026">
        <property type="term" value="P:chiasma assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF20">
        <property type="entry name" value="MUTS PROTEIN HOMOLOG 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="6">
        <location>
            <begin position="740"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4367566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="858" mass="94449" checksum="D4E9D0A864BE925B" modified="2023-11-08" version="1">MPTGPERPRVQAQRGPFLDSTSTHTPSEGTQRNTPGDDDESGDDTFDEVILAVDLTPRGTVGCCYYVARDEKLYFMEDIEFGDVGVVDSLKMFVNPTVILLSTRIDDAVIDHFDPEARRGSATGDNDQFRLPFLLEVRPPSEFSYDAAKRKVVNLRLGEEDGVRVSFNVPGELAAVDQIDGDNLIGQQGQLLRLAGWIDMESRVTVGCAGAVISYLQRRRAAAYLPGDQSAHLLFRISTLEMFSLRDTMFINADTLHSLRIMDAESHPHSHNRGPTANSGAKEGLSVYGLFHHLARTPQGKCLLRQHFLRPSLDLDVINERLDTIGVFVRPENESALQRLVRTLRSTGNMRMLLSNLRKGVGGSTKGRGGFSSRIWVAIRGFAFHALKIKDIFQEDLGADTLTIRTKIFEKFEGHHLAQVGSKVSETVDFERSAEEYRTVILPGVDEELDQMKRTLDGLESFLNEVARKLSERMPSDIRAALNVIYFPQIGFLVTVPLDPSTGAAIYDGGFETPWERMFSTDEMREMDDHFGDVYGIISDREIEISHELAQYVLQYDELLTSCSDICGELDSLLALAQGAKIYKLCRPRMTLDNVVRIGGGRHLLQELTVSSFVPNDTFLVGGAGDDVPLADGHRQHGTKSHWQSSSSTHSTYVHKGGPSMLVLTGPNYSGKSVYLKQVALVVFLAHVGSFVPADSAKVGLTDKILSRVTTRETVSRIQSAFMLDLQQISLALNLATHRSLLIIDEFGKGTESSDGAGLACAVMEYLLSLGAAMPKVLGATHFHVPAEIVQRAENLVLLSIKGEDLVAACCQMPESEAAELEDAERIARDFLEADVNKDPKGVLTKILTVSGTSESRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5N4</accession>
    <name>A0A9W8Y5N4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5N4"/>
    <dbReference type="OrthoDB" id="9402762at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005996">
        <property type="term" value="P:monosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43725">
        <property type="entry name" value="UDP-GLUCOSE 4-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43725:SF3">
        <property type="entry name" value="UDP-GLUCOSE 4-EPIMERASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="418" mass="45892" checksum="57BBEC9F115BBD8A" modified="2023-11-08" version="1">MVVGGLGYIGSHTVLELLKEGYNVLVVDDLSNSYEDVLSRISTLAIEYYATQEKTMPALHFRQLDYRSPAMKSVLASYASYSVSSKPAPIAKGFPFSQLHRTDSGIDMDMDDAEPTVERQSRISGVIHFAAYKSVEESIRMPLRYYSNNVCGLVDFLALLEEFGIKNLVFSSSATVYGEGANCGIPLREELCVHHPETFLDNDGSERQVLPGVLGLTSPYGRSKFMCESILADLAKSDPTWSITALRYFNPVGCHESGILGEDPRQKPSNLIPVIATVLTGAKPVLDVFGTDWNTPDGTAVRDFIHVVDLARGHIAALAASAAGRTKAPFRAYNLGTGRGHTVREVLRSLEQASQRKIPAREVGRRAGDVGFCVAEVQRAETELQWRAERTLDDCSGDVWNFTKARCPEIPETDSMVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5T6</accession>
    <name>A0A9W8Y5T6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6699 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006706</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5T6"/>
    <dbReference type="OrthoDB" id="5363135at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046522">
        <property type="entry name" value="DUF6699"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20415">
        <property type="entry name" value="DUF6699"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6699" evidence="2">
        <location>
            <begin position="70"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="179" mass="19737" checksum="5C5F1B6F281B4E2E" modified="2023-11-08" version="1">MAAAETDATLQKVDSLVEEPKDGAAKKGHRRVSSMAADVYNIEDLEKEKKEIKISAETQKLGWKLNKSSTTVEDPAVLKLPLTEPKLKKITLHFPLGLEVTARNLKGVTIKDALDAIHKQFKKRADDEFDKPFLAGFEWDPEECYTRFIVHQTNTPTSAMSGAPGGSKKKKKAAAEEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y683</accession>
    <name>A0A9W8Y683_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Syntaxin binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">sec1</name>
        <name evidence="3" type="ORF">N0V83_006551</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y683"/>
    <dbReference type="OrthoDB" id="2228at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="471"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="615"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="475"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="646"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="680"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="702"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="715" mass="81054" checksum="E2803CB1228E9D09" modified="2023-11-08" version="1">MSASMIDIQRELILNTIRQTTRGDWKILIVDEDSQKLINNVVKEDDILNSNITNIERLEERRTTQRDMDAIYILTPKAHIVDCIMAEFDQRRYRGFFLIWTSLLLPQLKERIDRSQMAREQIRSFRTVHLDYHPQESHLVTFRDPWSFPILYHPECNNLVVRHMEDIAEKITGVCVALGEYPIIRYYRPRNPTHEASVLCSHLARFVQEKLDMYAQFNQDFPPQSNRPRGALYITDRSMDLCAPFVHEFTYQAMAFDLLPIQEGDKVTYRTVVNEEDPGAEEKDMEISDKDKIWVENRHRHMKDTLGKLISDFQKFIADNPHFTNQDAQNAAGMNGLNAIKDMIAGLPQFQEMKEAYSLHLTMAQKCMEIFQKHKLPDLASVEQCLATGLDEDYRKAKNMADQVVRTLDEEEITPADRLRLIALYIIFKDGILPADLQKLLLHAQLPAPDGEVIRNLDLLGARVARQLKEKRDAPPPLFPPKAAPPPNTEDYSLSRFNPALQDMLEEHVRGTLPQDAFPFTKMSPDDASMMQDNAPAASLRSAKPTWAKSRLASVEPRQRVIVFVAGGATYSEARACYDISNKTSRDVFLVTSHMMKPQLFLRQVGDLSQNRRNLRLPVDQPPPTAPKHLFEKPEPPKPAPPPAGTKPIPGGLPSGPKVGGAGPKPPTAAMGAINLNAPSKQSPAPSPSSGGKYGKDDKDKEKKKKKLHLFSSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6R1</accession>
    <name>A0A9W8Y6R1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cytosolic Fe-S cluster assembly factor nbp35</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">NBP35</name>
        <name evidence="12" type="ORF">N0V83_006754</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NBP35-CFD1 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6R1"/>
    <dbReference type="OrthoDB" id="1741334at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904564">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly scaffold complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000427">
        <property type="entry name" value="Cytosolic Fe-S cluster assembly factor NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03038">
        <property type="entry name" value="NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028601">
        <property type="entry name" value="NUBP1/Nbp35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264:SF35">
        <property type="entry name" value="CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="84"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>ligand shared with heterodimeric partner</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>ligand shared with heterodimeric partner</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4368397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="343" mass="36730" checksum="74A77EA49C807D06" modified="2023-11-08" version="1">MAPSLEEPTQVDFDAPLKAAPKLVAPEPEHCPGPESQQAGQADSCAGCPNQAICASAPKGPDPDIPLIAARLSSVKHKILVLSGKGGVGKSTFSTMLSHGFASHPDSTVGLMDTDICGPSIPKMMGVEDETIHVSGEGWEPVWVKENLGVMSVQFMLPGRDDAVIWRGPKKNGLIKKFLMDVCWGELDFLIVDTPPGTSDEHLSVNSFLKASGVDGAVLVSTPQEVSLLDVRKEIDFCRKASIPILGIVENMSGFVCPGCKHESQIFRPSTGGARRLAEDENIPFLGAVPLDPRIGMACDFGESFLEAYPDSPACRAIREVVRRVGEEMGLAKEEVLPEEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6W2</accession>
    <name>A0A9W8Y6W2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_005876</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6W2"/>
    <dbReference type="OrthoDB" id="5393181at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028143">
        <property type="entry name" value="Get2/sif1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28263">
        <property type="entry name" value="GOLGI TO ER TRAFFIC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28263:SF1">
        <property type="entry name" value="GOLGI TO ER TRAFFIC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08690">
        <property type="entry name" value="GET2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="144"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4368794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="318" mass="33294" checksum="172D47B42D1A01F9" modified="2023-11-08" version="1">MADSPASPTGALPGESPAQTKARLRRERLANKSGASRLQQITALQGGPPKDVSEFEKDVPVKPSATPSQLTPAVSGTATPDPDEIDISQHHYTPASQPRLPSPFAFEANQGGPFAPGQVPDASQDPMMAMMQQMLGGGAGGFPGLPGGQGQSGAGPGDLPPGLANLFSAMNGGAAAAEPSPEQSSAWIWRLVHSVFSLGLAIYIVLQTPFTGSKLSRDNVLDDDWTGQSTPAQNFAHFFYLFATFEVVLQSSRYFIEKGQLQGSGILSTVAGILPAPYSGYVRTIGRYSVIYSTVVSDAMVVVFVLGATSWWRGAAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6Y3</accession>
    <name>A0A9W8Y6Y3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005377</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6Y3"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="104" mass="11827" checksum="317379545BD7FD4A" modified="2023-11-08" version="1">MFIPNLPIQPTNTQVAVTSVLEGTIPTILLGGLVMGFAVVYYSIRKKKEKKPRQAQQTMRHGRRRGSSSAYPPGLQIDKVLENWETVGMEEPFFIPDNRRGRRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y701</accession>
    <name>A0A9W8Y701_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Biogenesis of lysosome-related organelles complex 1 subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_005898</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BLOC1S1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y701"/>
    <dbReference type="OrthoDB" id="20018at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009395">
        <property type="entry name" value="BLOC1S1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13073:SF0">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13073">
        <property type="entry name" value="BLOC-1 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06320">
        <property type="entry name" value="GCN5L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4368816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="123" mass="13189" checksum="634E0E25AA99FAD3" modified="2023-11-08" version="1">MASPSGSEAARHTAEARAAVTASLSSVGASLDNEMRTRTADLHSNSAAISKQEKDLAKQTAALAKQNADLEKVLQGGNKTLNEVGDIQNWTEMLHRDLLVLEETIRLVDGKEEDETASGTRGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7M2</accession>
    <name>A0A9W8Y7M2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">U three protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">utp7</name>
        <name evidence="12" type="ORF">N0V83_006332</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7M2"/>
    <dbReference type="OrthoDB" id="10251154at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000378">
        <property type="entry name" value="U3 small nucleolar RNA-associated protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012952">
        <property type="entry name" value="BING4_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040315">
        <property type="entry name" value="WDR46/Utp7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14085:SF3">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14085">
        <property type="entry name" value="WD-REPEAT PROTEIN BING4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08149">
        <property type="entry name" value="BING4CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01033">
        <property type="entry name" value="BING4CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="290"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="BING4 C-terminal" evidence="11">
        <location>
            <begin position="378"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="529"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00076453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4369247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="558" mass="63088" checksum="F6E8F0F221CAB8FB" modified="2023-11-08" version="1">MATERGSAPETSQLRRKPSKGAPVEDEDARAIAQFTESATKQYGRGERIRPKSVKDKKLRANIRSLEAKNKQAAVEAKNVEILLENNAGLLEPEHELERTHRVRQDDIRREVGVETAKKGFELRLDNLGPYDVCEYSRNGRDLLIAGRKGHVATFDWRDGKLGCELQLNETIRDARWLHTSNQKNFAVAQKKCVFIYSGDGVEMHRLNFHADATHLEYLPYHFLLASASSTGILRYTDVSTGQSLPTIFTKLGPPTALAQNPHNAILHVGHQKGLVTLWSPNSSTPLVKLLPHHGPVRSIAIDKSGTYMVSTSQDRRMSVWDVRMFKEVHAHYLRVPGQTLSISDRNLTAVGYGTQLNVYKSDLFTHNAADIANPTMPYMSWGGEALSIGRVRFCPFEDVLGISHDKGFSSIIVPGSGEPNPDTMEAGTNPFETSKQRRETEVNALLEKLQPDMIALNPNFVGNLDLASDEQRRREYKAERGEKEEDKIERLKKRGRGRNSALRRFLRKSGSKNVIDEEKDRARAALEAQKRRNQDKREKMKKDYGPALERFAKRERF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7N8</accession>
    <name>A0A9W8Y7N8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006321</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7N8"/>
    <dbReference type="OrthoDB" id="5428890at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="442" mass="51194" checksum="C8BE9353A3698324" modified="2023-11-08" version="1">MALDRRANEDPIPESIRVEIIKGLWQCNTAGEHPIDIKRWDAFFSYYTAERKKAIAYSRGEYTTVRKHQDITAIANKLEGGCTKEEVTQSLLSLDTQQRSKEAKIRMVEGSLRLVARLISMVDIGPLPYCVQGRAPILWSNANSGLNSVLEEYFQKSSTSEQEIKFGEDFTAFNIQQFAGLQIQWTNNLANHLRVMDNDTTLCLFYHVTFLRHQNSAIFPEGLMEETLRTLALLFPRNDKNTRKWLLTDCFSTNNIFCLDMQILNGERVIGEQRRAETYEFWRDELLTLKEIFDKPRPTSLVHFWYDRRNKAQWYTFWIAVLILCLTIFFGLIQSIEDAQRRKALKELPKTLHESYDRILLRVVGFNAPIVVKTLQWFAFSTPYIEVDALLEAFSIDGEISIPDPEAQPTEEDLLTLCGSLIRKTNGHLELAHFTVLEYLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7R1</accession>
    <name>A0A9W8Y7R1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7730 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004974</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7R1"/>
    <dbReference type="OrthoDB" id="4757095at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056632">
        <property type="entry name" value="DUF7730"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38790">
        <property type="entry name" value="2EXR DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38790:SF8">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24864">
        <property type="entry name" value="DUF7730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7730" evidence="1">
        <location>
            <begin position="38"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="329" mass="37961" checksum="E7C33010597E346D" modified="2023-11-08" version="1">MLDNASLVTRNPSTMSSNSSLFSRIKPGIVSNKKIRGFLALPGEVRNMVYAYYFQTEVFCEFAASDYHFTQPTRPTVKLRSNAVSTSNFICTKDINQDDTMVVRVSRRLGKYNIVQGLHTNWLASVYALSLVCNQIHAETLPFLYNRTVFIFASPRRITNFLHVIPGLKLQQITKLRLHYSTYGAPKNVQDIIWQDKHTEAWKRALKAASKTLLNLQNLELWVQVSLSPLRLNLREQWVKPLLQFRRLNKLTTVNVDVQSHWSKGRWATFEGNKELARASWDLHELFGRALSLAILGAKEAEAMAEFNTAWEVTYRRWQYNLGYAKIGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7S4</accession>
    <name>A0A9W8Y7S4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005379</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7S4"/>
    <dbReference type="OrthoDB" id="19653at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF3">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="47"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="319" mass="34803" checksum="D67B2192447C0F53" modified="2023-11-08" version="1">MAAPTAPSTQLSSITLTWKEDPSAGPIPAHVYFPSATQNNDSHPIALLFHAGGFVLGTTDMIPKNQIANLVARGFVVVTPEYRLCPQVSLSDGPIRDAKEVYQWTQSALPALLLSQENPIKVDPSRIVAMGHSAGGQLALTTGLCPNPPRAIVDFYGCKYLDDESWTKPLPMFAAIPDQPQDFISKIYDGPQAITSLPMFVDGKPALSDPRCAWYIQQLKNGTSISSIVPDGDFARVDATKHFSASFPPTYFLHGGDADVFVNYRLSVRAHEELKGLGVETELVIGEEVGHVFDLIIQDTDPLFVKYVVPALDFLERHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y849</accession>
    <name>A0A9W8Y849_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006721</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y849"/>
    <dbReference type="OrthoDB" id="2107174at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044999">
        <property type="entry name" value="CbbY-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42896:SF2">
        <property type="entry name" value="CBBY-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42896">
        <property type="entry name" value="XYLULOSE-1,5-BISPHOSPHATE (XUBP) PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4368364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="248" mass="27382" checksum="A22A83D0F0F321F2" modified="2023-11-08" version="1">MGKAKYIMLDCDNTLVQSERLAFEACADLTNVVMEKHNIDARYTADSLLEDFVGHNFRGMLKGLQKKHNFTMPDDEMDDFVQQELGAVTKKLSQKAVECPGVTTELNKLKEQGYPMSVVSTSAKSRVVASIEKAGIAHFFPPEHVYSAATSLDPPSSKPDPAIYLYAAEQLGVKPHEAITVEDSKSGATAAMRAGIPCIGYVGIYGIEEGKEKMDQMAKLLTEETKCAAIMYDWKEFQECLKKVEAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y875</accession>
    <name>A0A9W8Y875_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006766</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y875"/>
    <dbReference type="OrthoDB" id="3205825at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35179:SF1">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35179">
        <property type="entry name" value="PROTEIN CBG02620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="316" mass="34775" checksum="5A34F9BADD192EFA" modified="2023-11-08" version="1">MIGTAVFVTAINISVFNIWIPARLQINHKYQVINEYWDRIEKGLYLAVDAYLNWYFLKTVKANLINNGLTKYNKLVRFNQQIVLVSLLMDVMIIAAMSIPNSFVYIQFHPLAYLVKLNIEMTMANLIKRIAISTSRRTGKASIAEEFKSSSNMSTSGQQTKSGTAGHQKSKRGSIHELASMVSYHADTKANGGGGRGASERVISFAPTGNQIKQTKEIVVSNEPNPFHDRRGSEVEITSGGGGMGYAVGSMGKTQGVLVDEKKLGLVRPKSLESVTEGNESLRSAAEVTKGKAEDYSDDEAALVGQKSRFWGRRQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8I5</accession>
    <name>A0A9W8Y8I5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005801</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4370037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8I5"/>
    <dbReference type="OrthoDB" id="3486565at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112:SF16">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="152"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="527" mass="59341" checksum="47AB40BA089C490A" modified="2023-11-08" version="1">MVGIEQFMSLLSRGELEDMRKIGLVDCWTRKYGPCRIGLTEIARDPDVSPLEHTNDDDDLGPLVAFEVFVSLTKTRPNSSAGLRTRDPLRVPAKLDVSRAVELARKWASECIQEHQCLTKNSVPLPKRVLDVSSSADNQIKLVTSEGEAGPYAALSYCWGAAKAIPKTTSSNLEQRHNGIAWSQLPRCFQDTILLCRALGLRYLWIDALCIIQDSKEDWEVESAKMANIYSSAYLTISATSCLDPSMSLFADRRVYTVLDTAAYSGSAYPSLPYVTCVEYEKRLFEFKHDTGTAHWRIMEPGISGLNEHRFAKTPDAPLLTRGWVCQERLLSARILYFHGEEMMWECRSGVRCECGVLDDDDTADINRPYQVWRRRLKESFAEALSPYADTQILRETWMDVVLRFSQLNLSYESDRLPALSGLAAQFADRNIGEYYAGIWTSMFPASLMYDLNPGPGLRDARSYMPNKEKYAPSWSWASLPLMGQNSVNYNWSKPRFFKSGCPSFTVISVSGGALGKILSGRSKMQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8X3</accession>
    <name>A0A9W8Y8X3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">type I protein arginine methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.319</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rmt3</name>
        <name evidence="10" type="ORF">N0V83_004426</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48096"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11991"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61897"/>
            <dbReference type="EC" id="2.1.1.319"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:48097"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:48101"/>
        </physiologicalReaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8X3"/>
    <dbReference type="OrthoDB" id="7848332at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035242">
        <property type="term" value="F:protein-arginine omega-N asymmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000003">
        <property type="entry name" value="Blast:Protein arginine N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.160.11:FF:000016">
        <property type="entry name" value="Protein arginine methyltransferase RmtB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF123">
        <property type="entry name" value="RIBOSOMAL PROTEIN ARGININE N-METHYLTRANSFERASE RMT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 7 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4371210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="507" mass="56137" checksum="4135ED150A18A250" modified="2023-11-08" version="1">MADSDSDTASSVGSIFEDASEPDTTSFKCLFCNQQWSRVSDMGKHCTTEHGFDLIKTVQSLGPDADELTIIKLVNYLRAESQKSIDPKSIDVSLKTLESDEYLLPVLEDDALLFELGDLMPDPGTKALDYDEFEAALHKDMPEDFSKIKLTNDRDQDYFESYKGNSIHREMIEDRVRTEGYRDFIEKNPGVFNGKTILDVGCGTGILSLFCARAGAKKVFAVDNSGIAIRAKEIVAKNGHQDRIEVIQGRVEDFNTQRLIGKEKVDIIISEWMGYGLLFEGMLDSVLRARDMYLKEGGIMVPSHCNVRLAPISDAEWTADSTSEKFWKDVYGFDFSPMVPGGLLNTHEIGVFDVPAQALCGSATSHLLEMKTVSVEDLSFTVPLSITLDREISSLHAIAIWFDTIFIHPSSSQDVKTLDSVQWGQNGIPGLGFSTGPSNTPTHWHQAVLLLDKDIADKQVFEKGTVLEGNLTYAKEKGDDRGITVTVEWKGHGVHGEVSGKVQRTMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8X9</accession>
    <name>A0A9W8Y8X9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8X9"/>
    <dbReference type="OrthoDB" id="5383784at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="329" mass="36312" checksum="5039939C48579D8B" modified="2023-11-08" version="1">MEHFVERWVYFVIYEAVQSSVIRPDNSDMQCRDAGDDDRGLRILGLRRPSPPLIRDAINKLLRVIGWGQPLASQSNERKPYTESAHTLDSNEGQTVDIGATTRVTNVTPLELALARAQDQLDTVPSVAESFTIPINALDDPVRPTTPPSPTASIPDQEDNDPRIRITNREGIVEMEVRLPPRILSTHTEVADISAPVRSRHRGASRNVPSISGNRPYHRVTQLSTEPAKFINAVVKAQIVGVALLPIRLVVRRLIASHYLAGQPGYAGLPRVVKQLPGLSDLDWRSIGVEISRLALCGALELTIDLGFWGLQYLAVTNLGKHIFGWGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA57</accession>
    <name>A0A9W8YA57_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Geranylgeranyl transferase type-1 subunit beta</fullName>
            <ecNumber evidence="9">2.5.1.59</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CDC43</name>
        <name evidence="9" type="ORF">N0V83_005145</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.59" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA57"/>
    <dbReference type="OrthoDB" id="24893at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005953">
        <property type="term" value="C:CAAX-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004662">
        <property type="term" value="F:CAAX-protein geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF4">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-1 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="8">
        <location>
            <begin position="10"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4370624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="445" mass="49623" checksum="EFE61A12D552B2B5" modified="2023-11-08" version="1">MAPVTSESQLNYARHVKYWRRNLKTLLPHFYTGNDANRMMLALFTVSALDILGDLDAALSSEERRGHTDWVYSCQLPEGGFRPWPGSNFGGLRDDGNKRWDPAHLPGTFFALLTLVVLGDDLERVKRREILAWLVKIQRPEGSFGETLGEDGFVNGGNDSRFGFMATAIRWILRGDLEGPWEGIPDIDVDSFVNCVRDAECYDGGISESPYHEAHSGFTCCAIAALLFLDRLPLKPGQKSDGRLRGVTNVPQTLHWLASRQTTTLDEDDGLDTYNDETDTSETCHDAHSFVKLGARPSKQADSNLKGRPHIHFELQWVGMNGRCNKVADTCYAYWTCAPLQILGHLDIINRAPIRKWLLDKTQHIVGGFGKVTGDPPDMYHSFLGLMVLAMFGESGLQSVDSALCITDRAKKHLESLSWRRKITGGVDLQDVSIRLAGGRMQVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAA0</accession>
    <name>A0A9W8YAA0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dienelactone hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAA0"/>
    <dbReference type="OrthoDB" id="17560at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17630">
        <property type="entry name" value="DIENELACTONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17630:SF105">
        <property type="entry name" value="DIENELACTONE HYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G08790)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dienelactone hydrolase" evidence="1">
        <location>
            <begin position="27"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="292" mass="32548" checksum="75FD0DC0BE443567" modified="2023-11-08" version="1">MSCPDCFRGGVSRSHSTGKEITIHGVKTYVAEPEASVTPKGIIVLITDAFGWDSVNNRVLSDHYAKRGFLVYCPDLMNGHAMSPSVITPIETLLEKTSWFTTIFVKWFLFLEVLYHVIPWVLTCTPSKTETHVIKYFQALRTSPPPFQTKDLKIGAAGFCWGGKHTVTLAKDDPKHRVIRHSSQTLSSAPEPLIDAAFVAHPTYIKVPDDIDPIKIPISWSVGEEDLQMKGPDIEKVKVLLESKKDAEHDVQIIPGAKHGFAVRIHPEDKEQVAAAERAEEQAVSWFSQCFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAK5</accession>
    <name>A0A9W8YAK5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_003908</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAK5"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF198">
        <property type="entry name" value="AZOLE RESISTANCE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="201"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="213"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4372135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="231" mass="24645" checksum="6BBE5D80FFC15C57" modified="2023-11-08" version="1">MSALFGATYFLPVYFQAVKGVKAITSGVYPLSMILPQMLLVIIAGATVNRLGYIPSFAITSGALTAVGPGLFSLFRPDTPTGEWVGSMVLTGVGGGLGIMMPSLFGPNILDLRSFLSLYNVIFDTSLRSQLRAKAPNANPQAIINAGATHFRYIVDDGDLPGVLVAYSNSIDHVFYLTAALAGVASITGWGMSWKKISKKEKEDLESTEMPDTEARSAEDDSTRNAKNKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAM0</accession>
    <name>A0A9W8YAM0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">COP9 signalosome complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_004076</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Component of the COP9 signalosome (CSN) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAM0"/>
    <dbReference type="OrthoDB" id="295656at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000190">
        <property type="entry name" value="COP9 signalosome complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054559">
        <property type="entry name" value="PSMD12-CSN4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040134">
        <property type="entry name" value="PSMD12/CSN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12/COP9 SIGNALOSOME COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855:SF2">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22241">
        <property type="entry name" value="PSMD12-CSN4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="9">
        <location>
            <begin position="197"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4372302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="417" mass="46257" checksum="76BE7ECB4A085219" modified="2023-11-08" version="1">MASSPVNAALQQIEAAPVSEKPAQYTSLLQQITASSKNLAADLNAYAQALLDDSLGIVVLRPLLAAFVDAFRAVQDPDDKIEVGERVISLLQSKGAGQYEEQDTQIKHALADAFEQNEDFRRSAQTLATINLESTQKSVSADEKAKVWIRIVRCYLEEDDPTSAFTHLNKVKNILFSVQDKETKLMFQLSQARILDSQRAFLDAAQAYYGMSNEMIIDEDERQDFLGKAIICTVLAPAGPQRGRMLAKLYKDDRASRAEDFAILEKIFLDRLLSPAEIKAFASKLQPHHLAKTSDGSTVLDKAVLEHNLLGASKLYNNIGFEQLGELLGIDAEKAEDYAAKMLEQGRLAGYIDQIDRLIYFEGEASGERKTGHAERVVGNELRKWDTNIAGLAEEVEKVASAIQNQYPEFYASQVVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAM7</accession>
    <name>A0A9W8YAM7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_003149</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RENT3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAM7"/>
    <dbReference type="OrthoDB" id="18087at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12455">
        <property type="entry name" value="RRM_like_Smg4_UPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000637">
        <property type="entry name" value="Nonsense-mediated mRNA decay protein Upf3, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039722">
        <property type="entry name" value="Upf3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005120">
        <property type="entry name" value="UPF3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13112:SF0">
        <property type="entry name" value="FI21285P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13112">
        <property type="entry name" value="UPF3 REGULATOR OF NONSENSE TRANSCRIPTS-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03467">
        <property type="entry name" value="Smg4_UPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="472"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="164"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="546"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="191"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="347"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="390"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="415"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="577"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="589"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="609"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4372858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="620" mass="65160" checksum="DF28A93B544FE38B" modified="2023-11-08" version="1">MAPPAGVNGILPVTVLQKNAPTQAPRGPKPAQPRLKLICRRLPPGLTKAEFESILGDEWKLGAGKIDWLHYRKGNISTDAAKPSRPARAYLHVTKQEHVKVLGDLVRAATFHDAAKSWQDAALVGLPTLEFAPYPKMPGGRRRNDNRQGTIDQDQEFKDFLESLTNPITKPPPPENEANKEKVKTTPLIEALREKKANRDKPSKANRQARGEGKEDAAEKKIMSKSGKENALNTGDKNRRLTKADKAQATKEAVKILNKEASSSKTAAANTSADKSTASPAPERRRGNVALAKSMLQRDLGVGPAPTRRRGTKREVASAVPDTATQAKASVAPDQKQKETVAVQPAAANTSEQTAQAHKKERPTRAERRAFKAGLTDKSNNKAGNEDAKAQTPTKAPAPQILKKPQPAPSPSAPKGPAASRAPPTEPAAARNPPAPTPQQTNAARSEATHTTPANVAPKAAPAPPAPAPTSKQAFLKHANPSQGITEPLIEEALKAFGGIEKVEIDKRKGFAYVDFADLQGLQKAMAASPIKIAQGAVQVLERKEKVARAPNPRFNGPPTGPARGGRGGFAPRGRGGRGGARGGGHIAGGPTTAAGGSTPASPAPAPAVPTAVASADVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAP1</accession>
    <name>A0A9W8YAP1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005759</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAP1"/>
    <dbReference type="OrthoDB" id="4463286at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="388" mass="42488" checksum="2A1B89E1F24C5A7C" modified="2023-11-08" version="1">MSPPTRDVMSNRPSSSRSRGPAPRIVVKKEPASPDMPSTTRHRPQRLNLSNNIVHSSGALTARPSGQNSANLQDMGLACLSPGFHTQDPTMREQLQRSLDVRERQRQIIEARQKHGKGGPPEGEDVRNTEVGAFGRTIKTPQTSRRKGPPPGLSIAPPSHQHFASDRVIQSAPINQTFTGLRGNAPPTRQLANGPSNLSQTSHIHHVPAHQTNNRLPPISDVFPEPLGTSRNIYSTSSPGHSSHSNIQPPMPSPGFPPNQYQQQPPPTSRPREYKSAEEAVQSLSGGREDLLPRLVHYGGHQPPTPPSPMPNKNGSSAYPSHTDLHRSGSTRRRGRDEYEQDNGTPPLGRQQARLGPFGAGRDSPETQLKKKEEFIGLCARAWDLFHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB20</accession>
    <name>A0A9W8YB20_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin-like fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004286</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB20"/>
    <dbReference type="OrthoDB" id="37297at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33875:SF2">
        <property type="entry name" value="ACR183CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33875">
        <property type="entry name" value="OS09G0542200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13462">
        <property type="entry name" value="Thioredoxin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin-like fold" evidence="1">
        <location>
            <begin position="19"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="210" mass="23348" checksum="152C64175E0D6EE4" modified="2023-11-08" version="1">MALAPKFAGQKLASSAIQPKAVHTLELYLDYVCPFSAKLFKTVYSTSLRTTLLEKYSDRVVTIFRQQIQPWHPSSTLVHEAGYAVQRVDASKFYAFSAALFEQQKDFFDVNVVNETRNATYKRLAKIAGSVGVDEGKVYGLLEIGDKPGEDGGLNTGNGVTDDVKVQVKQNRLVGVHVTPTVVFDGVVNNEISSGWTTQQWEEWLEKNVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBD2</accession>
    <name>A0A9W8YBD2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phytase A</fullName>
            <ecNumber evidence="4">3.1.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Histidine acid phosphatase phyA</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Myo-inositol hexakisphosphate phosphohydrolase A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Myo-inositol-hexaphosphate 3-phosphohydrolase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">N0V83_003406</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1D-myo-inositol 1,2,4,5,6-pentakisphosphate + H2O = 1D-myo-inositol 1,2,5,6-tetrakisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77115"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57798"/>
            <dbReference type="ChEBI" id="CHEBI:195535"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:77116"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1D-myo-inositol 1,2,5,6-tetrakisphosphate + H2O = 1D-myo-inositol 1,2,6-trisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77119"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:195535"/>
            <dbReference type="ChEBI" id="CHEBI:195537"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:77120"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>1D-myo-inositol 1,2,6-trisphosphate + H2O = 1D-myo-inositol 1,2-bisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77131"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:195537"/>
            <dbReference type="ChEBI" id="CHEBI:195539"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:77132"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1D-myo-inositol 1,2-bisphosphate + H2O = 1D-myo-inositol 2-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77135"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84142"/>
            <dbReference type="ChEBI" id="CHEBI:195539"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:77136"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>1D-myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16989"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57798"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="EC" id="3.1.3.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:16990"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histidine acid phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.8" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBD2"/>
    <dbReference type="OrthoDB" id="6509975at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016158">
        <property type="term" value="F:inositol hexakisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016274">
        <property type="entry name" value="Histidine_acid_Pase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963:SF24">
        <property type="entry name" value="3-PHYTASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000894">
        <property type="entry name" value="Acid_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00616">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00778">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="18">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="18">
        <location>
            <position position="394"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="111"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="247"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="298"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="468"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00043670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00043748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00043788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000894-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000894-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ4373115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="499" mass="55608" checksum="3C0D46FD4381058E" modified="2023-11-08" version="1">MMARAWNRMRGNRAQEYAPLPLSEKEELPSVQSRQGSRSKNGPSGLIWHVVGGLFCIFSLISLLIALSRNKSCDTIDDGYRCSPKTTHFWGQYSLWYSVPSDIDVAPPKGCDVTFANVLSRHGGRDPTLGKSIAYALLIAEIQNTSTTYPDKFKFLKDFNYALGADELTDAGRQEMVNSGAHFYRRYQKLVEKHTPFVRAGGQHRVVESGEKWLAGIAQSLKSEPNNIDVVIPEGPAWNNTLSHDTCPAFEGGPNHGLGDRAQQVWASQFVPPIQERINSALGTNLSATSIVYLMDMCPFHTLAHPTAKVSEFCHLFTEKDWHGYDYFQTLGKFYGYGVGNPLGATQGVGYVNELLARLTETPVEDHTTTNSTLDSDPATFPLDRKVYADFSHDNDISGILAALGLYNKTQLLPNTTIQDTKKTHGYSAAWTVPFAARMYVEKLQCKHEKEEFVRVIVNDLVHPLEFCGADKYGRCTLSKFVESQSFSRSGGLWSQCYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBU9</accession>
    <name>A0A9W8YBU9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">tRNA (Guanine-N(7)-)-methyltransferase non-catalytic subunit trm82</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TRM82</name>
        <name evidence="8" type="ORF">N0V83_003922</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. In the complex, it is required to stabilize and induce conformational changes of the catalytic subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat TRM82 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBU9"/>
    <dbReference type="OrthoDB" id="339900at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106004">
        <property type="term" value="P:tRNA (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03056">
        <property type="entry name" value="TRM82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028884">
        <property type="entry name" value="Trm82"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16288:SF0">
        <property type="entry name" value="TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT WDR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16288">
        <property type="entry name" value="WD40 REPEAT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="435"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="453"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4372149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="486" mass="52902" checksum="3AFC904BD2C21D2D" modified="2023-11-08" version="1">MALPYQCLVARAGQGTNLKDWSLFGASGSALVAQSSNGITSVWSEQDARRRDDQQDDQQDDDTQGPPVKRLKLEQPKEPKANFTNLVLSNSGQYLIGVTGEDKCIRVFQIDAQNKLQQLSERCMSRRPCSVTLTSDDSTILCADKFGDVYALPLLAPPADDESGSRVTPQPEPKEKDYTPAATALTVHSGRNRRTLEEQLKQKAKGVPKSKEAMKFHHELLLGHVSMLTDIVYAKSGSRGYIITSDRDEHIRISRSQPQAHVIEGFCFGHEAFVSRLCLTQSGLLVSGGGDDYLCVWDWQNYRLVGRLPIRNVALDHLVTRSEVTTAHVDAANFKGDEILVACEGLPALFSFRIGTAGTGATAIALDGNALDVALVQSGLGSCMAVVSIDNVHKPGSTTEGRADKNLSRLQCFSSQRAGHWQQDASMPELLGGFESRSAGEVDGRKSGAQAEEGMRPGSADDKAVRATQTLYYHVENLRKRPGAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC69</accession>
    <name>A0A9W8YC69_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_003714</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC69"/>
    <dbReference type="OrthoDB" id="294295at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052178">
        <property type="entry name" value="Sec_Metab_Biosynth_SDR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618">
        <property type="entry name" value="7-ALPHA-HYDROXYSTEROID DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618:SF12">
        <property type="entry name" value="OXIDOREDUCTASE, SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY (AFU_ORTHOLOGUE AFUA_1G14540)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4373419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="287" mass="29854" checksum="441193616A5EDC73" modified="2023-11-08" version="1">MASAQSDVQLQDFANIFSLKGKVAVVSGGSRGLGLHAASGLLQAGCSKVFITSRKASACDEAVAALNALQNKQPGAKAYSVPADSSKLSEIERLVEEVAKHTDHVDILFANAGATWGSYFDKVDEKNGWDKVMDLNVKGVFFTIQKFAPLLEKAGSVEDPSRVIVTGSVAGIGTGSLGESGAYSYAASKAAVLHLARNLAVELGPRHILVNGIAPGFFMSKMAAVLMERSGGADALNKSNPNGRVGKPEDIAAAVVFLSSRAGGHVNGDTLVLDGGKIWGSNKLERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCC8</accession>
    <name>A0A9W8YCC8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_004642</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCC8"/>
    <dbReference type="OrthoDB" id="269227at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF115">
        <property type="entry name" value="DEHYDROGENASE XPTC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00623">
        <property type="entry name" value="GMC_OXRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040995298" description="Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein" evidence="5">
        <location>
            <begin position="21"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="6">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="7">
        <location>
            <begin position="303"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="554"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="597"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4371425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="630" mass="67801" checksum="A15A9914515C7B2B" modified="2023-11-08" version="1">MSFIIKSIFFFASFVPQIVNQSSGGSRGEPDVAGKTFDYVIVGGGLTGLTVANRLSEDSSRSVLVIENGYAVDDITTQVPSFANSINVGLMYDIPSAPDANTGGSHPVWVGNVIGGGSMVNGMAFDRASAADYDAWEALGNPGWNWNSLFGYFKKSTTFTPPTATNVKEFGTSYDASAYGNGPVQASFPNFEYPDMKTIWASWKAEGVPILKEHALGNAVGAYWSPTSLQPKTQTRSHARNAYYDPVKSRSNLVLVTGKTVNEILFNQGLLTGYTANGVQYKSRLDGSVNKVYARREVILAAGSIFTPQLLQLSGIGPKSVLDAAGIKVKKDMSAVGANMQDHPNANMVYDLKNMAFPNPLSGQDPAYNASAWAQYQSNRTGPITQAHGSSLAFLSLQTVSKKSTQIVNAVKSQSARDYLPSVYSDSALLRGFSKQRDIIANLHSRSDAAVGEFPMVAFGLAISSLQRPLSRGTVTLNPSNKYGNPVVQWNTFQNPVDKQILAEMVRYTRQHWARPELSKFSPVELSPGVQAQTDDEIMEGLISQGGLEASFAHMSGSCSMMPENYGGCVSSDLKVYGVNGLTIVDASIIPLIPATHLQATMYAVAEKAADIIKARNVLNILPKLTALWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCP0</accession>
    <name>A0A9W8YCP0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF2264 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002908</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCP0"/>
    <dbReference type="OrthoDB" id="5150166at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049237">
        <property type="entry name" value="DUF2264_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049349">
        <property type="entry name" value="DUF2264_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016624">
        <property type="entry name" value="UCP014753"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35339">
        <property type="entry name" value="LINALOOL DEHYDRATASE_ISOMERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35339:SF4">
        <property type="entry name" value="LINALOOL DEHYDRATASE_ISOMERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10022">
        <property type="entry name" value="DUF2264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20938">
        <property type="entry name" value="DUF2264_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF014753">
        <property type="entry name" value="UCP014753"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2264" evidence="1">
        <location>
            <begin position="24"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2264" evidence="2">
        <location>
            <begin position="381"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="658" mass="73796" checksum="ADC2192AB9D0064B" modified="2023-11-08" version="1">MTAKTADANRAFDLHPFSTNPFKTRDDAVAAAASLLDPLIPGFSPKNAVIKCGSTGTRFDETGAQVEGFARPLWGLAPLLAGGSKYAATDKWVQGLISGTDPESEEFWGYMEDTDQRMVEACPIGFTLAVASKEFWDPLTDKQKKNVETWLGSMNDKKMPDTNWLWFRVFANLGLERNGAQWSKEQLEKDIKQLNSYYRGDGWSNDGPAHYRQMDYYSSSFAIQYLQLLYSKLAADIDPGQAEEYKKRARMFALDFVHFFDPEGRAITFGRSLTYRWAMVAFWGAVAYSGVELPEPLTWGVVKGIWLRNLRWWTTQQDIFQSNGMLTVGYSYPNYYLAENYNSPQSPYWCMLAFAALAVPESHPFWSAKEEPHPFANQRVVRSLPHPRQIVIRSGGHSFLLSSGQSCHYAIKNSNAKYGKFAYSAAFAYSVSTGYQELEQFVPESTLALSDDGGELWKARRALNGDSEIETHDGVPVLVSSMKPWPDVDVLTYLIPPAEDTPNWHLRVHRIRTGRDVMTAEGTWACYGARESDGRLLTEWNGTLRTLNASIPEGGHSDTVSAFAASPRTGAVGIVEIYQGMRTGSVILSDANSNLVEARTVLPIVRKDLKAGETAWFVSAVFALPASVKGWKESWKEGWEKRPKVPKWVQDKISGRWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCV6</accession>
    <name>A0A9W8YCV6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002998</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCV6"/>
    <dbReference type="OrthoDB" id="3800277at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="423" mass="48517" checksum="2BC7EE9F80C82A47" modified="2023-11-08" version="1">MSRHVSVSRNFVNLGKLYGFHNGDVVPYIRSAQVPINFVNLGNLYSPTKNQWPPSWAYFEDGSVCIFPYKWLPHGEVERRMNLAAQFVNNPTPKIQVIRQQDPQRGRLILIKRHLTSAQFMDAPQLPLKRLRRRLQSSEALETFRGTLVNRTLQPNFRLGNGLVMSSNPDEVNHPVRGIRRIQDDETKALRESPSQSEGTPATLVHQLPNSPSQNPSKLARRVSQDQEAQGREKARPVRKRGSPPPLDSSSFGPEQDIKRREAEVARRERELIQIQQQLLLQTTRKHNERNPRDAAPRRSHLDDMEAEEQEPKQRVKILQLKRKPLALERQLAMDIEGDSSPTRVPHHNDLDSLLHELENGHIHSLTEETSSEPQHEEVEEESAPAAFDINDAEPPAALAKLRSRIQSPTRRTLRSKREPRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDQ2</accession>
    <name>A0A9W8YDQ2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha N-terminal protein methyltransferase 1</fullName>
            <ecNumber evidence="5">2.1.1.244</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Translation associated element 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">X-Pro-Lys N-terminal protein methyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_002952</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N-terminal N,N,N-trimethyl-L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54712"/>
            <dbReference type="Rhea" id="RHEA-COMP:13785"/>
            <dbReference type="Rhea" id="RHEA-COMP:13971"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138057"/>
            <dbReference type="ChEBI" id="CHEBI:138315"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-terminal L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-methionine = N-terminal N,N-dimethyl-L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54736"/>
            <dbReference type="Rhea" id="RHEA-COMP:13787"/>
            <dbReference type="Rhea" id="RHEA-COMP:13974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138059"/>
            <dbReference type="ChEBI" id="CHEBI:138318"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N-terminal L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N-terminal N,N,N-trimethyl-L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54724"/>
            <dbReference type="Rhea" id="RHEA-COMP:13789"/>
            <dbReference type="Rhea" id="RHEA-COMP:13973"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138061"/>
            <dbReference type="ChEBI" id="CHEBI:138317"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. NTM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.244" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDQ2"/>
    <dbReference type="OrthoDB" id="1298661at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071885">
        <property type="term" value="F:N-terminal protein N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000025">
        <property type="entry name" value="N-terminal Xaa-Pro-Lys N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008576">
        <property type="entry name" value="MeTrfase_NTM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12753">
        <property type="entry name" value="AD-003 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12753:SF0">
        <property type="entry name" value="ALPHA N-TERMINAL PROTEIN METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05891">
        <property type="entry name" value="Methyltransf_PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016958">
        <property type="entry name" value="DUF858_MeTrfase_lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="146"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR016958-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4374211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="263" mass="29275" checksum="C6253ECB8A139E75" modified="2023-11-08" version="1">MDMDSDPPLIAPHDPNRPDASINVTHSLDYWNNVSSDVNGMLGGYPQTSRIDLQGSSNFLTKLRRGKSQASKQPLAPLERAADCGAGIGRITKGLLLGVARRVDVVEPVKKFTDELVQSLGNGEYAGDGEGKDGKGQVGEVINLGLQDWIPEAGAYDLIWNQWCLGHLTDAQLVVYLRRCKDGLRHAKEGEEERNSWVVVKENLSTDIMHKDIYDEEDSSVTRSDDKFRRLFKDAGLKIVATEQQRGMPKELFPVRIYALRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEB2</accession>
    <name>A0A9W8YEB2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001129</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0696 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEB2"/>
    <dbReference type="OrthoDB" id="10067381at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015034">
        <property type="entry name" value="Bles03"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31977">
        <property type="entry name" value="UPF0696 PROTEIN C11ORF68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31977:SF1">
        <property type="entry name" value="UPF0696 PROTEIN C11ORF68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08939">
        <property type="entry name" value="Bles03"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="244" mass="27017" checksum="2B860BB57DE0E14C" modified="2023-11-08" version="1">MAEGEMVSGDGWVSDESSFYGDDDEQEHQLNLCDNFNPKSCWSTDHKDLNAVLRAAKAQQPSMVLSKETISFARQSSSGGLSTTADKLSAFSTLDRKTMEQERLLRLGKRERDTTPEPQTKVELVNPRQGHFDSWQLGESVDDFVNRLHPLSTSALTCPWIWVENPHRKPGAICASPQVEHFTNLGLDLLEHSLQTRQEMQGKGSRGAKGTLTKLLDIESKALQQCIADLAAEDQVLSGKVSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEB4</accession>
    <name>A0A9W8YEB4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEB4"/>
    <dbReference type="OrthoDB" id="1028014at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34776">
        <property type="entry name" value="F17F16.3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34776:SF1">
        <property type="entry name" value="F17F16.3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="512" mass="57173" checksum="F3EE85E6F52AB645" modified="2023-11-08" version="1">MTGTRSSARNAAKGSSPAKSDDGVAGTKRKPEAEPSPKRSRKATKKQATIEETLGSKQEDDSEMKDATDNEQEQDVQEMDQSDEKPDDEQTQSQGGDAKEQEGTGNAKEENTSSDAKNSAANGDDPIEKSSQRAKDVPSNILEKGIIYFFTRNRVGIEDADSVADLQRTFFVLRPIPTGAKLGDGALTDQKNNRLLALPKKAFPKSHNDRFMAFVEKANTTVQDLKENFFAANEYETKTQGTRRTEPITPVAEGVYAITRTEDATTHLVYSTTIPAELGEVQEDLGIKSQGSFIISVKNPERSGPAAAQLPQKPDFPKEIIEEFRNLAWTEVKPKYLDYEYCQILLIGEKIDKAVEPTKKDQKHDKETPQEEIDKLEHEDELRIQHLHGDDSVFDDLKISKDEYPKYNLESIKRKAAEAIIDRLPFMHDALSTVDLAICFHEQFPQIDLGLRKATIKLLQARLPAIMEDELAWKEFSDNKVLLKAFHAYQCEMADSESSTSVPTPPATPPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEC4</accession>
    <name>A0A9W8YEC4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002413</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEC4"/>
    <dbReference type="OrthoDB" id="4186058at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="300" mass="34262" checksum="A13B11D54DE10EE2" modified="2023-11-08" version="1">MATHDQPIAAMLNIHPTSPPMTPGPRTQNRYPPQTHQTSPLYKRMGMSKMAQTPPLEFIEPTGKGHQFNFTRPQSLEPSSRPSTPSVRAFATTSEDSEDDDESEEESEDEEETKPLRRAERAHFVLEELDSDPGYDCDIEVVRPDHYEDAKSDRSGTKSEVFARLKELVLEEDSSDEEERERKYRQKKKRWSAGIFKRSHSQSVEGDSSYSDNDPLDDVDPTARRLRRRVRGPCDRRGSLIFEDRGFSNTNNIAEVEEPDEGRVMHSKGPPSIPSDDAFTLDELPFWRGGDLMDVEFESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEN4</accession>
    <name>A0A9W8YEN4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">APL2</name>
        <name evidence="9" type="ORF">N0V83_002105</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEN4"/>
    <dbReference type="OrthoDB" id="10254310at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030117">
        <property type="term" value="C:membrane coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000058">
        <property type="entry name" value="AP complex subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016342">
        <property type="entry name" value="AP_complex_bsu_1_2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002291">
        <property type="entry name" value="AP_complex_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="8">
        <location>
            <begin position="3"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="610"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="626"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="673"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4375026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="702" mass="77029" checksum="1B1A43FFC4FEEC52" modified="2023-11-08" version="1">MSMTLGKDVSALFPDVLKNIATADLDQKKLVYLYLMNYAKSHPDLCILAVNTFVQDSEDPNPLVRALAIRTMGCIRVDKMTDYMEEPLRKTLRDESPYVRKTAALCVAKLFDLAPQMCIENGFLEQLQELVGDPNPMVVANSVTALAEISEAAPETKALAINSTTLKKMLLALNECTEWGRVTLLTTLADYKAADVKEAEHICERVVPQFQHVNPSVVLAAVKVVFLHMRHINPDLMKSYAKKMAPPLVTLVSSAPEVQYVALRNIDLLLQKQTDILSKEMRVFFCKYNDPPYLKMQKLEIMVRIANDKNVDQLLAELKEYAMEVDMDFVRRSVKAIGQVAIKIESASEKCVNTLLDLINTKVNYVVQEAIVVIKDIFRKYPGYEGIIPTLCQCIDELDEPNARASLIWIVGEYAEKINNAGEILSNFVDTFAEEFTQTQLQILTAVVKLFLKKPDQAQGLVTKVLQAATADNDNPDIRDRAYVYWRLLSSDPQIAKDIVLSDKPPITSTIRSLPPQLLETLLAELSTLASVYHKPPEAFLGQGRFGAEAMQRAAIEEQQEEARENPIAAAAAAAAVSGQAPTTNMENLLDIDFDGSAPASLQKQPIQGESGLEGLAGTPQRVASPATNAPAQPQSNMDDLLGLFGDGGGGAAGAPSAQMSNDDIMNGFAGMSLQSNQPPPATQQLGSEPSKKSNQDLLDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEW0</accession>
    <name>A0A9W8YEW0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BD-FAE-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002237</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEW0"/>
    <dbReference type="OrthoDB" id="19653at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049492">
        <property type="entry name" value="BD-FAE-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF3">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20434">
        <property type="entry name" value="BD-FAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BD-FAE-like" evidence="2">
        <location>
            <begin position="22"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="295" mass="32656" checksum="1347CC883B607F90" modified="2023-11-08" version="1">MASVLPTQTITYKSVGSLEIPLDIYLPPNARNAPVLLWFHGGGLLQGRRDLLAPHMKRGVEKYGFACIAADYRLAPQADVAEILEDIKDCIDFIRKDIALHIGEGLVDGSRLAVSGSSAGGFLTLLAGLYVEPKPQVILPIYAITDPLGTFFTAPRPAPNGRYSASKEEMERHLDANAQAISSSGKVPDDHRSHMYVYMLRNANLAELWGIPDEKRAYPYRISRNIHHHRLPPAYFLHGDADSAVGVEQSDEVVGAMLGLGLTVEYERPHGKDHFLDDGPDYENEALYSFLMRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF30</accession>
    <name>A0A9W8YF30_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enoyl-CoA hydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_002521</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Mycotoxin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF30"/>
    <dbReference type="OrthoDB" id="2139957at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000104">
        <property type="entry name" value="Related to enoyl-CoA hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF68">
        <property type="entry name" value="CARNITINYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0843">Virulence</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4373782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="29973" checksum="2ABD3EE4C492133F" modified="2023-11-08" version="1">MSTTKPQTTPPQTSHVLLSYPADYVLLVTINRERQMNSIPFEGHIEMGQVFDWFDAEPNLRVAIVTGAGKKSFCAGQDLIELGKIRSGQLKVNPGLMRHPLSGFGGLSRRQGKKPVIAAVNGFALGGGFEIVLGCDMVVAAPGATFGLPEALRGIFAGAGGPPRLVRNVGIPVASEMALTGKPITAHRAKELNLVNRISPSQDTVVDEALKLANDIAAISPDAAIVTRAALKEAWETGSVERATQLTIEQYNGKLQASENALEGLKAFAEKRKPSWKPSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF66</accession>
    <name>A0A9W8YF66_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Acetyl-coenzyme-A carboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ACC1</name>
        <name evidence="21" type="ORF">N0V83_001864</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP = N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13501"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hydrogencarbonate + acetyl-CoA + ATP = malonyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11308"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="14612at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004075">
        <property type="term" value="F:biotin carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.460.10:FF:000001">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000005">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000010">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.460.10">
        <property type="entry name" value="Biotin dependent carboxylase carboxyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1770.10">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049076">
        <property type="entry name" value="ACCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049074">
        <property type="entry name" value="ACCA_BT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013537">
        <property type="entry name" value="AcCoA_COase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728:SF3">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08326">
        <property type="entry name" value="ACC_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21385">
        <property type="entry name" value="ACCA_BT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0092">Biotin</keyword>
    <keyword evidence="9" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="18">
        <location>
            <begin position="63"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="17">
        <location>
            <begin position="215"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="16">
        <location>
            <begin position="698"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="19">
        <location>
            <begin position="1522"/>
            <end position="1864"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="20">
        <location>
            <begin position="1868"/>
            <end position="2183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ4374788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="2287" mass="254717" checksum="5FFD562B6BE81804" modified="2023-11-08" version="1">MGIPTDNMAGAAQNGVNGSANGHPSSYAAKHSLAPHFIGGNHLAAAAPGKVKDFVTAHDGHTVITNVLIANNGIAAVKEIRSVRKWAYETFGDERAIQFTVMATPEDLQANAEYIRMADQYVEVPGGTNNNNYANVELIVDVAERMNVHAVWAGWGHASENPKLPESLAASPKKIVFIGPPGSAMRSLGDKISSTIVAQHAKVPCIPWSGTGVDDVVVDDKGIVTVQDDIYEKGCTKTWEEGLVAAEKIGFPVMVKASEGGGGKGIRKVDRKEDFEQLYKAAASEIPGSPIFIMKLAGSARHLEVQLLADQYGNNISLFGRDCSVQRRHQKIIEEAPVTVAGSKTFQEMEKAAVSLGRLVGYVSAGTVEYLYSHADDKFYFLELNPRLQVEHPTTEMVTGVNLPAAQLQIAMGLPLHRVKDIRLLYGADPHTSSTIDFDFSTEGSDKKQRRPTPKGHCTACRITSEDPDEGFKPSGGTIHDLNFRSSSNVWGYFSVSSAGGIHSFSDSQFGHIFAYGENRQASRKHMVVALKELSIRGDFRTTVEYLIKLLETPAFEDNTITTGWLDELISKKLTAERPDPLIAVVCGAVTKAHVASEACISEYKTSLEKGQVPSKDVLKTVFPIDFIYEGYRYKFTATRSTIDSFTLFINGSKCAVGVRALADGGLLILLSGKSHNVYWKEEVGATRLSVDGKTCLLEQENDPTQLRTPSPGKLVRFLVENGDHVGKGQPFAEVEVMKMYMPLIAQEAGMVNLIKQPGATLEAGDILGVLALDDPSKVKSAQNYLGLLPDLGAPQIMGSKPPQRYVYLYNILQNILQGFDNQVIMQTTLKELVEVLRDPELPYGEWNAQASALHARMPQKLDTTFSQIVDKAHSRSLEFPSKALSKAFQKFLDENVAKGDAELLRASLAPLAEVITRYSEGLKAHEYSVMLKFLELYWAVEQLFSSRTSRDEEVILKLRDENRDNITSVVHTVLSHTKVNAKNNLVIAILDLYRPNKPGVGNIAKYFKGTLKKLTELESRQTAKVSLKAREVLIQCAMPSLEERTAQMEHILRSAVVESRYGESGWDHREPNFEVIKEVVDSRYTVFDVLTQFFVHQDPWVALAALEVYTRRAYRAYQLQNIDYHNEGDQQCLLSWDFILRKVGEAEYGLAVEPSEPGTPGTPGFERPPRIQSLSDMTAWQNRFEGEPSRKGVVVPVEYLDDADELIAKALDLFPAIGISKKSGISLKEGLSLKRTPTSGATEFKHSDELTGVLNVAVRDIESNDDAEILERILPIIQEYKDELLVRRIRRISFICGHKDGTYPGYYTFRGPTYEEDASIRHVEPALAFQLELGRLSKFDIKPVFTENRNIHIYEAVGKGAESDKRYFLRAVVRSGRLREEIPTAEYMVSETDRLMTDILDALEIVGTSQADMNHIFINFSHVFPLDPAEVEEAIGGFLERFGRRLWRLRVTGAEIRIIVTDPQTGIPYPLRVIITNTSGYVIQVEMYAEKKSEKAGKWLFHSIGGTTKIGALHLQPVSTPYPTKGALQPKRYKAHLMGTQYVYDFPELFRQATENSWVAAIAKHPFLRDRQPAKGECLESYELVLDDTDNLAEVNRDPGHNTIGMVGWMVTARTPEYPRGRRFIIIANDITYKIGSFGPQEDNFFHKCSELARKLGIPRIYLSANSGARIGLAEELIPHFSVAWKDANKPEAGFDYLYLTPEKYEHFVDGKRNDVICEKVEADGENRYKITTVIGAEDGLGVESLRGSGLIAGETSRAYEDIFTITLVTCRSVGIGAYLVRLGQRAIQIEGQPIILTGAQAINKLLGREVYTSNLQLGGTQIMYRNGVSHMTAEDDFEGVSKIVKWMSYVPDKKNSPVPISPTADSWDRDVTYYPPGKSAYDVRHLIGGMEDEDGWRSGLFDRGSFEESLGGWAKTVVVGRARLGGIPVGVIGVETRSVENVTPADPANPDSIEQVTSEAGGVWYPNSAFKTAQAINDFNKGEQLPLMILANWRGFSGGQRDMYNEVLKYGSYIVDALVKFEQPIFVYIPPYGELRGGSWVVVDPTINPQFMEMYADEDSRGGVLEPEGIVGIKYRKERQLDTMARNDPKYGALKRKLNDPATPKDQLQSIKAEMTEREKHLLPIYGQIAIQFADLHDRAGRMKAKGVIRQALRWQNARRFFYWRLRRRLNEEYILKRFAAAAAPASRDTAKVDPITRARGLEDLKHVSKIPNWENDDKSAAEWYEENRQLISERVEKLKTEGIAQEIATLMRKDKEGGLKGVMNVLSTLETADKEQVLKMLSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFK2</accession>
    <name>A0A9W8YFK2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001099</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFK2"/>
    <dbReference type="OrthoDB" id="5372935at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4375822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="312" mass="36105" checksum="32617E06555668DD" modified="2023-11-08" version="1">MRANKQVHNEVVEYLYEKRTLFMIVARDRESPTLTNEYLSRYYETLASMPTQTRQHFTKLEIQIGYLSSQTFTAKRHTHVPSVSDPMSHILFLLPDLDTVVISFQSLMYTSVSTHRIVRERTQTAEWLIKYIPESVNVVWDLARAFAFRTKLDEQPMWRTIQARGEINLGHSVFTQLECVRNSYAEQKQLKSLGSFDPVNKQSHALNFWVANYGFGIDGLDDLDYDHSHLILPYWHRVPADSSLRLALLAVAHAAFGRARSSDAAIKKATNLYHRTLTRVKDEIEAVTVTNIEQLLLAVKLLESFEVRVERF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG71</accession>
    <name>A0A9W8YG71_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001698</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG71"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050817">
        <property type="entry name" value="DjlA_DnaK_co-chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24074">
        <property type="entry name" value="CO-CHAPERONE PROTEIN DJLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="156"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4376415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="230" mass="26005" checksum="CE36D290647E4BFB" modified="2023-11-08" version="1">MSLSSPKHPTSNSDQPQVHAAIPVRRRRRPAPLFTIHRDPDVENAPATPAPRCTTPNPRKPLAEKTDHGNSTPSPSPRAPDTPFTLSPLDPNWEDFENYNPFLTPPPTPLGPSRMQSPLPSPRTPTASSRHIPRTARRRRAAVVAYYPRIAPTHMYLYEMMGLNDWKVSTTAIKVAWRKKALQLHPDRVVEEQRERATLDMQLLNAAKEVLCDSARRAQYHLDGKMPWVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGP3</accession>
    <name>A0A9W8YGP3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">Alkaline phosphatase</fullName>
            <ecNumber evidence="2 11">3.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_000585</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.1" evidence="2 11"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGP3"/>
    <dbReference type="OrthoDB" id="7392499at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.140">
        <property type="entry name" value="Alkaline phosphatase, crown domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018299">
        <property type="entry name" value="Alkaline_phosphatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042085">
        <property type="entry name" value="Ap_crown"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596:SF5">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00123">
        <property type="entry name" value="ALKALINE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="6 9 11" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040872507" description="Alkaline phosphatase" evidence="12">
        <location>
            <begin position="21"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="8">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="423"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4377755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="496" mass="54310" checksum="2CEBE0B671F7CE8E" modified="2023-11-08" version="1">MRYALYLTVAGTLLSQGASAIWLLPQARNFIYIVPDGFGSASQTMARDYVSLLQNGENPEAPVSIQLPADQMVIGNVRTLASDNLITDSAASATAFGCGVKTYNDAVGVDAAGQPVGSILEAAKGEGYRTGLVVTSTINHATPACYAAHVADRNSYEKIAEHEIGYSHPLGPQVDILLGGGRCYFKPRSDPTSCREDDIDLFGYAKKKGFRVMQDRKAFDELKKGTSKAADLPYIGLFNDDQMMYEIDRKQQPEKEPSLLEMVETALTSLDRATKWSYRGYFLMIEASRIDHAGHANDPVGHLHDIIMYNEVMKFVREWIDKHPDTLMMSAADHECGGLSLNGFNPLPLKGATMTTEHAERLWNAYNGTDRRAYLKDTILPGYALGDLSDSEIDTILANNDLSSDLSSRLSKKAGVNWSTSGHTASDTVLYGYGSGWRGGNLKADMAGNWDNTQLPGYIEAVLKVKMSKVTEKLRKAGSAWVGKRDLSSHGHIHKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGS0</accession>
    <name>A0A9W8YGS0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_000271</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGS0"/>
    <dbReference type="OrthoDB" id="2152119at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659">
        <property type="entry name" value="HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659:SF42">
        <property type="entry name" value="UPF0057 MEMBRANE PROTEIN ZK632.10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01309">
        <property type="entry name" value="UPF0057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="5"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="114" mass="12159" checksum="61C9F8086D59D652" modified="2023-11-08" version="1">MIATLLLIIITIFIPPIGVFLVAGCGVDLLINILLTLLGYFPGHIHAFYVEYVYIKRRDEVRAGIYNARPAPGVYSQKVQSGGHHGAMPVAAAAGPAPVPAQQGYPQQGYGTVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGU4</accession>
    <name>A0A9W8YGU4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGU4"/>
    <dbReference type="OrthoDB" id="3798269at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02178">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00929">
        <property type="entry name" value="ATHOOK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="434" mass="46160" checksum="29BBB1B7272B6C41" modified="2023-11-08" version="1">MATKATETVSRGRGRPKRAAAPEPAPVTIQSPPVKRGRPTKDAVEVVIEAPRKRGRPAKVADEEPALMSEEAPAPRRRGRPSKAADDELAVEAPGPKKSAGRSRKEDAPVKDAHLDAAAPTLKKRGRPAKSTSSPTLNGVMGSPRVTKRASTRIKSAPTATATAAASALAVRRIDPRMRSRLRTRLPPPVPQVAQEEPVRKPTKRGRPRKDVAKVPAPKSTTAGLKALNKASKAGIEKQAKVTKTVAPRKRRGYTTIEVPDKFAAQMKEFLQNLLDGDSSDSAPVEAGEAEEQEDEIVVEEDVIVGNATGGDEYHDDGLPNGLPNGEVEQEKDETSDIEIALQAAAADDSITPDAGDGETTAQAAYTADDLVTPEDAYIENDASEEPHAPSGVGGLTVSELDEFEYLESLANGDDGSQMDVFQWANIFSPYSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHJ1</accession>
    <name>A0A9W8YHJ1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHJ1"/>
    <dbReference type="OrthoDB" id="10524193at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="305" mass="34466" checksum="5B2D99241F6BDCA4" modified="2023-11-08" version="1">MTSQGYRKLEFRTRRMACAYTHDGDDVWVTFLKRNKSLAPGKIARMYLHTAPKLNHTAANLTVDDIDFATTSPQLGKYVIKYTFHDIALAVWSWYGDDLELKTWEELEGRCTVEAGYESDCSETSDKHRHRLESRTGFDHNEQDNNSNTTTDTLTTIPKEMTLDEAIDIQNDSFNGKDVDEDQLALSFEEHGHIKADQFTDLAAAYTDGGAVAEQNSPRASSKRSRASDDDEGSSSPMNKRARLDSEESELGDGSKTWMECTGSLGMTVEQDHGHERQAVDLEKRAELLGLPMSWWYDLEDSCRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHW7</accession>
    <name>A0A9W8YHW7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">STE20</name>
        <name evidence="17" type="ORF">N0V83_000846</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHW7"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019236">
        <property type="term" value="P:response to pheromone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06614">
        <property type="entry name" value="STKc_PAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000011">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000385">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000012">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF22">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SAMKA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="16">
        <location>
            <begin position="218"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="554"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="123"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="310"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="343"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="450"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="496"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="583"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4378016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="835" mass="91202" checksum="7C51D83873901BDA" modified="2023-11-08" version="1">MEDTRTKASKRLTKRERLPPPVAYLNKSSSKSNTLKRAPSAASYFTSPNQGLGIQSASLSADTQAAGSSLRRSPSLTPVHEHQRAANAKYPPAQAPSPSIAHLPPASVGEPFKAIVEEALYASHSSNTAGPRRPAGPQHSQTADQPPRFRSPNLRQSASFNTLVRKMDTPPSGTKSPRNRYSDDGDTAKKRRSDGGKKKGTFSTFMSSMLGSPRRPTISTPTNPMHVTHVSIDNETGEFTGLPKEWQRMLQQNGITEQEQKQHPQAAVDVVNFYKDNAEKSEEDAIWDKMGPAHPQGYAYQANIANNYGSVQPLLSPPQSPRFPRNDHDSFENPRAPPPIPRSLTSPVPNTPQLNGSLVPNRPAPRPPAAAPGSANVIPTRNAPPAPSPIVTQQPTPRQENDYPQIAYAPTPISDSPIGTSSAPRSRANTTGGTPPRFDSPASATGISPAQQYHQQQQQAMAAAQQPKAPLGRSVSQRAPQPAHFTPAAPAPSQPSPQQQFAQQTDPQNIPLPSQQARVGPAPRPRQRPRQSQGPDIVAKLNAICTNADPTLRYRNLNKIGQGASGGVFTAYEVGTNKCVAIKQMNLEQQPKKDLIINEILVMKDSKHKNIVNFMDSFLVRGDLWVVMEYMEGGSLTDVVTFNIMSEGQIAAVCRETLHGLQHLHSKGVIHRDIKSDNILLSLEGNIKLTDFGFCAQINENHNKRTTMVGTPYWMAPEVVTRKEYGRKVDIWSLGIMAIEMIEGEPPYLNESPLRALWLIATNGTPTIKEEHALSPVFRDFLGFSLKVDPDKRASAHDLLVHPFIQTAEPLNTLAPLVLSARKARAEERRKKGGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJK4</accession>
    <name>A0A9W8YJK4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_000951</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJK4"/>
    <dbReference type="OrthoDB" id="5286008at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045290">
        <property type="term" value="F:D-arabinose 1-dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070485">
        <property type="term" value="P:dehydro-D-arabinono-1,4-lactone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19164">
        <property type="entry name" value="AKR_ARA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000037">
        <property type="entry name" value="L-galactose dehydrogenase (L-GalDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044480">
        <property type="entry name" value="Ara2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686:SF1">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="3">
        <location>
            <begin position="40"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4378118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="461" mass="50960" checksum="E91DAAD2B73091DC" modified="2023-11-08" version="1">MATTPVEHPTTSSTFPTRQKPIPYSLVRGRRTPLSSILPPLVFGTATFNHQYNDDPFALDTTGLVTSALTHGIRAFDTSPYYGPSEQLLGNALSTPFVRETFPRNSYMILTKVGRIASEEFDYSKEWVRQSIKTSLERLHTEYLDLVYCHDVEFVSPAEVLEAVRELRRIRDEEGTIRYVGISGFPIDVLGDMAELILRETGEPLDAVQSYANFTLQNQTLAGSRGIQRLRNAGVDVVPNASILGMGLLRRQGVPVGALGDFHPAPSTLREAVRKASDFCDSYGERIEVIAIRFALETWISAGSICGSKGDPASGVPWKHESNDDVGGLKLGVSVIGVSRAPELEKTMLVWRSILDGLEGGRDTAAQAGRWYRAWEWSRNRRKAVQILAEGVQEVLGEWFGYAWASPDPGYVNKRKKDDGGKVRELVEQSEAPWLTPAASPQCAPVAGLAEVREERDLPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YK84</accession>
    <name>A0A9W8YK84_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fumarate reductase</fullName>
            <ecNumber evidence="4">1.3.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_000860</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Irreversibly catalyzes the reduction of fumarate to succinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>succinate + NAD(+) = fumarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18281"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.3.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="4">Binds 1 FAD per monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.6" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YK84"/>
    <dbReference type="OrthoDB" id="10252157at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016156">
        <property type="term" value="F:fumarate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050315">
        <property type="entry name" value="FAD-oxidoreductase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010960">
        <property type="entry name" value="Flavocytochrome_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01813">
        <property type="entry name" value="flavo_cyto_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43400">
        <property type="entry name" value="FUMARATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43400:SF12">
        <property type="entry name" value="FUMARATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0274">FAD</keyword>
    <keyword evidence="1 4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="5">
        <location>
            <begin position="7"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4378030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="462" mass="49184" checksum="DDAAECEFEED36DE4" modified="2023-11-08" version="1">MASRYAIVVGSGLAGLSAASQLISRNIPVRLLERGLKPGGNSIKASSGINGAPTVYQPVTEPPDAFYADTTRSAGHAMTSFTTQREKLIETLTKNSASAVEWLVQEQGVDLSRVAQLGGHSYARTHRGAGQTPPGFAIVSALLKQLKESPLFDLRTSCTVTDVLRDGNQVSGVAYTSEDGQKEELHGPVVFASGGFGGDAEGLLAKYRPDLAGYPSTNDPRPGTQPLLTAIGAKLIDMDQVQIHPTGFVDPTNKASPLKFLAAEMLRGEGGILLLNGKRFVDELDTREKVANAITSTAPTSQSPKQWTVQIVLDEGTYQAAKSHVDFYVFKGLVKKTTISELDPQALNSIAQYAKVVAGEATDEFHRTTFANWSLKEPKPESIVYVGVVTPVIHFTMGGALINANSEVLREDNEPIKGLWGAGEVTGGVHGGNRLGGSSLLECVVFGRIAGNQCADYMKSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHF9</accession>
    <name>A0A9W9CHF9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010404</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHF9"/>
    <dbReference type="OrthoDB" id="10256524at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04842">
        <property type="entry name" value="Peptidases_S8_Kp43_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051048">
        <property type="entry name" value="Peptidase_S8/S53_subtilisin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034058">
        <property type="entry name" value="TagA/B/C/D_pept_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43399:SF4">
        <property type="entry name" value="CELL WALL-ASSOCIATED PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43399">
        <property type="entry name" value="SUBTILISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="7">
        <location>
            <begin position="197"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5 6">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5 6">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5 6">
        <location>
            <position position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4362311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="695" mass="74982" checksum="618F92D086E8BF03" modified="2023-11-08" version="1">MSHSITVNGRSVALSHDLDASDTDYIILRTKGSPLNKRQKSELQKLGVAIEEFIGDDSDQIYFCNFKDNSLDQINNLDFVDYVGVYANAFAVPDVVKAEAHAGTGNIDVLLQHDVTELSDELLQKIAEAAEVDVDSTVVADGGVQLKVEVDNLEKIAALDEVRALHAVNEPALFTNVARQILRFDNAATSCSTVYKGTDQIVCVADTGLDKGSITDVHEAFAGRLKQLFSWGRLENNICDDLDGHGTHVCGAVVGDGQHVSQGLIEGTAPGAKLIVQSLFSGFNVLNQCRLGGVPGTNLAPLFDQAYQAGARVHTNSWGSPLPPTRIQRPYDGRAESIDLFVWEHQDMTILFAAGNDGQDVDLENKLDGTVNPRSLGAEASAKNCITVGATENLRPSLVPGDSNRPYTYGGFCSKRFAENPLRDDHMANNPEGLAAFSSRGPTAEYRLKPAFVAPGTAILSARSQNKKYLAAVNQAGASGDDKYMYLSGTSMATPLVAGTCAVLREALLANGYQDESNGVKNPTGSLIKALLINGAVTIRGQYMPHDVSDEPNPHSGFGRVDLARSLPKLNDPYSGYGIGTVDEDDEAEFMVEIPVPEPKVNDGKLCLKVTLAYADLPGGQLQNDLNLAVSSGNYERHGNQSEPFPIGTTVPFDRRNNVEQVVWPDVSTALVQVITKPFRFMSGRVPFAYAWQFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHL1</accession>
    <name>A0A9W9CHL1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010036</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Component of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHL1"/>
    <dbReference type="OrthoDB" id="3798148at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="364"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="367"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="397"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="427" mass="49052" checksum="00BFAD109E870D5E" modified="2023-11-08" version="1">MARKMMTARKETRQPKATSRVTGTTSAGVKKPKPKSKPKPEPPNQTTKWRIRRILAERTMAKETKYLVDWLPNWEPEGSLAGAAIILKEWEQNKKKDRTFTFNGDEVLKSTNETEDASSEFVKRMLHTIAREFKKFMSKNPAELARDLFKDEDWEFARSKYQQHAKNLAGKDKESTPTAAEVLRRTYIRMRSLNKHTFEKADLHYGAVKLKYVGQIDPSMRPETPDKLLKLAKTRPASSAAAFLSPLFSPVLLSIDPSHWKNDTAHRHLRALDPIVRMLPTQAPYLLSMTHVWPLFLTRLFIFADDISPMVQNVGITVGQEWPEKTRDELMWNYLKVSEGVDGRSVDCVERTYLEARDTVMWFVDGRDEEEGGTEEEDEDEDGNGGSARRQPKVMWDDGDGEKGGEEDEGFEEGRRVRLRGCRMMRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIP5</accession>
    <name>A0A9W9CIP5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_009184</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIP5"/>
    <dbReference type="OrthoDB" id="10256463at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039765">
        <property type="entry name" value="Yip5/YIPF1/YIPF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822:SF2">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="7">
        <location>
            <begin position="63"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4364588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="265" mass="28956" checksum="E2A7E932B3F47F54" modified="2023-11-08" version="1">MANRGYDVVVDVDQEGDLGHTDLQEDLEFHSSTRKHYLWTLSFYAQAFDVDTAEVLRRCVSTIYPRLNFLDVLEGNPDLYGPVWIATTVVVILFLTGTINQYLAHTGKGHFAYDFKLLSGAAGLVYGYTASVPVALWAVLKWYGSESANLLECACLYGYANLVWIGVALVSWSPVAILNYVFVALGLAFSAFFLLRNLYPVLSTTEAKTSKILLIVVLALHTGFAIAIKVLFFASTSPVGPGKAGDGDKGEGEAKGDMLSKMFAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ04</accession>
    <name>A0A9W9CJ04_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009324</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ04"/>
    <dbReference type="OrthoDB" id="409122at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04056">
        <property type="entry name" value="Peptidases_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030400">
        <property type="entry name" value="Sedolisin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050819">
        <property type="entry name" value="Tripeptidyl-peptidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218">
        <property type="entry name" value="PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218:SF19">
        <property type="entry name" value="SERINE PROTEASE AORO, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G10250)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51695">
        <property type="entry name" value="SEDOLISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S53" evidence="2">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="184" mass="19652" checksum="4EADA448C6364EDD" modified="2023-11-08" version="1">MDEITSVGATTLLNSSIYGGEQAVASPKGLLSYYSGGGFSSIFPRPSWQSKAVAKYLDKYAPKYGESVFNSSGRAYPDVSALGLKLATVYLDQTLGVGGTSASAPIFASIINLLNEERLEQGKGPIGFLNPIIYKYPEMFNDVTIGSNPGCGTQGFPASPEWDPVTGVGTPDYNKMREVFLKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJF0</accession>
    <name>A0A9W9CJF0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008192</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJF0"/>
    <dbReference type="OrthoDB" id="3930290at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="187" mass="20534" checksum="9F45834421F24B30" modified="2023-11-08" version="1">MARAGHSGTVVGSAVLVREKYYWPDLQLNIWTIIMLATAGTILGVNAQFMGIQDRMNLGTPWIMPYGVTVGALAIIFIIIEIVFIAQRKLLPGTMMLLSFILLVLFIAGIIGTAIQLFAGPNINNQCNTYVFGDGTNGANTNTLAFLQQRNICQCWQAVFAFWIIGSVFLVWMMVMASQVNSNTYAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJU2</accession>
    <name>A0A9W9CJU2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome maturation protein SDO1/SBDS N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007891</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJU2"/>
    <dbReference type="OrthoDB" id="2567806at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1250.10">
        <property type="entry name" value="Ribosome maturation protein SBDS, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036786">
        <property type="entry name" value="Ribosome_mat_SBDS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039100">
        <property type="entry name" value="Sdo1/SBDS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="SDO1/SBDS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927:SF2">
        <property type="entry name" value="RESTRICTION OF TELOMERE CAPPING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01172">
        <property type="entry name" value="SBDS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89895">
        <property type="entry name" value="FYSH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS N-terminal" evidence="2">
        <location>
            <begin position="9"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="114" mass="12349" checksum="7315C78B9EC1D7A6" modified="2023-11-08" version="1">MPRGNAAHTQVFYKGSSGDVFTVFVESAEAVKKWKDDSSVPLADVVAGWKIMVPEHGKQGILNTASNGQLENEFGTKNEDEVIKKILQQGEIQTAENPERQGSTNDSMGSMAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK61</accession>
    <name>A0A9W9CK61_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_006350</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4367995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3918771at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126">
        <property type="entry name" value="ANKYRIN REPEAT, PH AND SEC7 DOMAIN CONTAINING PROTEIN SECG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126:SF65">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_20, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="68"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="737"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4367995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1384" mass="155210" checksum="95E813DD9138B27D" modified="2023-11-08" version="1">MADTAASITATAAEPRKVKSPGEEDETEDDLSFDILAFMALYFDTMEDISKDPRQLTLSNTFFDARLLSLASGGGSGGSFAVTVVKSEDMARLQRKHDLEKDVSEILPQLIALRVPKMSTSTVQQQKQRILTAMAKEVAVLTHDEVRACDNVVKLIGACWSYANPEKTVLLPVLVYEAAPLGDLDAFLLSHPNLPLGHQLQICVDIAQGVERLHRVGVVHCDIKPKNIIMFSTTNPKRPYKAKIADFGCAIMLDDVETSARPPPGTRFWNPPEQLSGELVNKDTLHRVDVFTLALVIVTVLTSNQSTNTLMHINNRRPFMFPTEGRQMSFDYFKRWGSLVLSGLMILERFSNLTVTQRSSIKATQQERLIPAHLLDDDNWLDYSEQWNKHIASVMFRALQGDISHRDASVRDIASVLEAILVSVEVWGQRDHIRTDLHEVITSDNTTVSMTYVFEHESEGLMERARPLIVCKDMERMLLVKPGWMFRPHPEMDVRYVESRIAGTHGIEEWEEVIFGPERVGKRPSLTVQKRAGEVVKLTNYLGNLAHMPVPLLTQILHVLDGVANDSLEPLKRRSIAAYELALGLIAYCIDESRDDGDEEEVARALEFLNFALENGHEEVKWTVGWLYESFGKHYVHEKLEWLSQGMLAGSRMAARRLKQLDATNHYETRRRIQTEFQGIGEGWSIRPEYIQTEPIWFEGYPIPENNQVVLFAAAGMKNRVEALLNNGVDIDSTDGMRETALIAACRSGHVEMVEYLIERGADASLVSKENISALHFLSSFDKDKIPYIALLLLAAGCPLETHSSAHSNLNNGTTGADRFYGLVDGTPLLWAVAAHNITAMEILLDFGADAFDLAQRSSTNFPHSPVSWATSMHQAEILEQLLSAKTKHELSSLLNPSPNTFNSPFRGPPLGPAVAYSGGLVLYRIQLHGSQHHEQCRKTIHLLLSKGADPGRVGNSNEPALHAAGLHGQPYAFRALLDWNNGCLKPDTKIWVEILIGAVHNDRKSTFRELLKIPLETDEKYSWPRIICTVAGMPNSKYYLNKLISHYQELSNTPADYTMAFEEALQNECFENARIVFERADRCDIVYRRYEEEDDSSPARESMTLLGRLMRQAIIYASKLQAVREFLALTGSREILFDTVWDVQMGRYNALQVAVGYFPHGKTKTLTAGLLELILQYFKHPTRHLACIQASKSRSLLHLAVRYGNAAAVEILLGYPSVDVSHRDDQAGTAFDRALNRLQDSWRDKELAYWEVASEQLEGAREEWDNHTRTMIHVMQAKGASKHSTYSHVFKRLSEEIVRRWMVFPDDGRVEVTDFKVDTFVSGSIADKVKALPVGRCSYLSAFADPQVARQMPGASELDVGDAWDGGQVRGDAGLLGADGRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKE9</accession>
    <name>A0A9W9CKE9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pumilio y domain member 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">puf6</name>
        <name evidence="6" type="ORF">N0V83_007020</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">RNA-binding nucleolar protein required for pre-rRNA processing. Involved in production of 18S rRNA and assembly of small ribosomal subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKE9"/>
    <dbReference type="OrthoDB" id="497380at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012959">
        <property type="entry name" value="CPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040059">
        <property type="entry name" value="PUM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13389">
        <property type="entry name" value="PUMILIO HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13389:SF0">
        <property type="entry name" value="PUMILIO HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08144">
        <property type="entry name" value="CPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PUM-HD" evidence="5">
        <location>
            <begin position="158"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="682"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="110"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="127"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="693"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4367437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="723" mass="81057" checksum="28A9DEF32256347B" modified="2023-11-08" version="1">MAGIKRKSVVPSAAESKTKTKKAKVEKPTSKRDSKHEVKPLKKSKKVQEESSEDFDDSDSSEQENGTHDFQADEDEDEDMSDADSGEGEELETTKQDGKAHKRKDEQKSKTNAPDSKLSELNSNNSREAHAKQKALAKERKAAKPNADIIERTKKLWEKLRLKSHIEKDERKKLVKELFEIITGRVKDFVFKHDSVRVIQTAIKYSTMEQRRMIARELKGEYKVLAEGKYSKFLIAKLVEKGDPEIRDLVITEFYGHVKRMINHPEAAWIVDDIYRQIATQEQKNRLLREWYGPEFSIKGLAIQGTDSGELSVILAESPEKRKPVMSYLENQINSLIQKKLTGFTMLHDAMLQYFLVCEPGTEQANDFLEHLKPDPTLKEGEEADNVDLLKNLAFTKSGSRLMSLCFAYGTAKDRKIFLRPYKDTIETMAYDVHAHHVLLAALAVTDDTKLSSKSIYSELLPSNDTLPEKVINMVNDARARTVLLYPFAADAKWLLDDNTRERLAELYTIRQKTSKKDPNTRLQEIAKNIEPQLLSAITARAADFASFSFGLQFMGEVLVGAPEVEPSKRKEALAEVARLSQSILDAALPASAGDNKATSHGKNMLKTLVQGGKFDPSTKKVVPVEPALGFAVLFWNEIKSNVVDWATGQGSFVIVALTEAENFDKKDEVLKALKKAKKQLEAAANPTGGQEVEAKSKNQKKGDKAPVTIRGNAGARILLEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL61</accession>
    <name>A0A9W9CL61_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PRP22</name>
        <name evidence="15" type="ORF">N0V83_006622</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL61"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21691">
        <property type="entry name" value="GH2-like_DHX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05684">
        <property type="entry name" value="S1_DHX8_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000061">
        <property type="entry name" value="ATP-dependent RNA helicase DHX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000001">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000101">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000191">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049588">
        <property type="entry name" value="DHX8_GH2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049621">
        <property type="entry name" value="S1_DHX8_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF85">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="250"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="561"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="742"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="79"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="148"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1005"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1010"/>
            <end position="1022"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4368266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1215" mass="135909" checksum="DCF2AB4A9D954CB9" modified="2023-11-08" version="1">MDDLANLEFLSLVSKVTSEIQNHVGVSDKTLAEFVIDQHADCKGVADFKTQLEAMGAEFPQSLVESIDRLVLTLHPKYKNKNKGANGKTAGANGEDGAQETDRKTRVFRGLAIPDKEVDYEVPDDEPANGAPQADALDDTFAMLEGLAGKAPSKPTERSRKRSMSPFDSDDDASRSKRHRRRERSFSRSPSRSPRRRERNDELVFQDEFGRTRTVKPSNKQRSRKKYRDEDLDEFRRPPTPELDDEPVLYKVYDGKVTGVKDFGVFVNLQGVRGKVDGLVHVTQMAEGRVNHPSDLVSRWQPVKVKVIKMEGGRISLSMKEVDQRTGRDLEPGKRIAAFASGANSQGLGGAPGADITVPVVEDGYNGRKNGMRKRMTSPERWEIKQLIASGVIPKSDYPDIDEDYNAHINGEGGFEEEEDVDIEVREEEPPFLAGQTKQSLELSPIRVVKAPDGSLNRAAMAGDTLAKERRDLRQQEAQEKAAKEAANVDLSSQWNDPMAQQRQFASDLRNTRTNQPSEALPEWKKISTNSRETSFGKRTNMSIKDQRESLPVFKFRKQLLEAVAAHQILIVVGDTGSGKTTQMTQFLAEAGYSNELVIGCTQPRRVAAMSVAKRVAEEVGCKLGNEVGYTIRFEDCTSPDTRIKYMTDGILQREILLDPMLSKYSCIMLDEAHERTIATDVLFGLLKKTLKRRPDMKLIVTSATLDADKFSEYFYKCPIFSIPGRTFPVEVMYSREPESDYLDAALVTVMQIHLTEPAGDILLFLTGKEEIDSSCEILSERMKALGPNVPELMILPIYGALPSEVASRIFEPSPVGTRKVVIATNIAETSLTIDGIYYVVDPGFVKQSSYDGKLGMDRLQITPISQAQARQRSGRAGRTGPGKCYRLYTEAAFQNEMLPTTIPEIQRQNLSNTILMLKAMGINDLLHFDFMDPPPTNTMLTALEELYQLGALDDEGLLTRLGRQMADFPMDPSLSKSLIKSVDLQCSDEILTIVAMISATPSVFHRPRDKQQQADQKKQKFNDPSGDHITLLNVYNGWRSGGFSTPWCHENFIMPKNMLRVRDVRNQLLQIMARHKHTVVSCGRNTIKVRQALCSGFFRNSARKDPAEGYKTLVEGTPVYLHPSSSLFGKPAEHVVYHSLIETTKEYMHVCSAIEPKWLVEAAPTFFKVAPTDRLSKRKKAERIQPLHNKFAGEDDWRLSAQRRAGRGGGGTWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL68</accession>
    <name>A0A9W9CL68_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycoside hydrolase family 30 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_005930</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL68"/>
    <dbReference type="OrthoDB" id="2012278at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004348">
        <property type="term" value="F:glucosylceramidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006680">
        <property type="term" value="P:glucosylceramide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033452">
        <property type="entry name" value="GH30_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049161">
        <property type="entry name" value="GH59_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001139">
        <property type="entry name" value="Glyco_hydro_30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11069">
        <property type="entry name" value="GLUCOSYLCERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11069:SF23">
        <property type="entry name" value="LYSOSOMAL ACID GLUCOSYLCERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17189">
        <property type="entry name" value="Glyco_hydro_30C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02057">
        <property type="entry name" value="Glyco_hydro_59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040768849" description="Glycoside hydrolase family 30 protein" evidence="4">
        <location>
            <begin position="24"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 59 catalytic" evidence="5">
        <location>
            <begin position="131"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 30 beta sandwich" evidence="6">
        <location>
            <begin position="336"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4368848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="432" mass="46153" checksum="50887D3AF8282261" modified="2023-11-08" version="1">MQLTLAHLCALVVAPSLTSATSAALPLKGRQAQQKVTIDVTKKYQTMDGFGISGAFQRANLIMNLQQPKQREVLDLLFNTTTGAGFSILRNGIGSTPNSNKDYMNTILPKCPSTPAEVTVSGASCRSGDWKQAYANYLIAYINFYASAGIKVTHLGFLNEPDYTTSYASMGSSGIQAADFIKVLRPTLDRNNLTSVSINCCDATGWNVASQHASQIAAAGAESMVYAITSHEYTSRISGTIRTNARVWQTEYSDLNGGWSTAWYSNGGSGDGFTWANTVFNGVVNSNLSAYIFWEGVQDRATNNNNNEKLILVDGQTYQVSKRLWAFGQWRVVRPGGQRIAASGGGSNIKSAAFLNIDGSVAVVIINSAASAQTISIAPLGFPVVQAWYTDNTHDMATVEATLGSDGTASASVPSRGMISFLFKKAANGTMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLM7</accession>
    <name>A0A9W9CLM7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_004880</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLM7"/>
    <dbReference type="OrthoDB" id="4525710at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF25">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="172"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4370362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="587" mass="65308" checksum="CF7875F7BD602B92" modified="2023-11-08" version="1">MPSCIRGNAFRFRPVKAVKFNAGEDIGSAEQSLEFRAGQTWELVLAQTADLARFVVRFIAPNTDEDDEAHVIEDEVNRELEIASDAAGDDLLIAQPSRGSIVERNDSASDNVLLSYQDRSPFPGITVPTFHSTSNLPSIHDVLSNHISPTSTTHNASSADCIVPKDVNSASTSDLLPTTDSWHGSPSIQKKSPLESNNNISPYNSSAFSPSPGINLRWPVNNAYEARLFHHYIIYCTNWIDVCDSRQHFEKEVPKRATHFPVILNGILGLAARHLWLMGKVAEDRSQPYVDQCLQALIVALEDPLAHWDENFLVAVILLRLHEEMGDADDQCHHFVLVTGTARILNSISSFAADGGLRESASWVSLRQHIYVSLTTQQTLNLSLENYRHSSVFREFDDEAWANRVIFLFASVLQCVFEDSGTNSLDKNKWKELDAEVDEWERTKPWTFSALHVEPDAGEKFLGSWPDLPCAQGVVAVGLQYYHLCKILLTIYSPNASLVGLVGVRARKAADASIRKHMRITIGYGVSNSHCGNAMFQGSHILSACGAYVVDQREQDACVEYLTGLQKLIGWRTDKVLADLREQWSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM89</accession>
    <name>A0A9W9CM89_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_005659</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM89"/>
    <dbReference type="OrthoDB" id="4188028at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013907">
        <property type="entry name" value="Sds3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01401">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="385"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="526"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="636"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="689"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="176"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="213"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="404"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="642"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="709"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="725"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="827"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="866"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="897"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="949"/>
            <end position="962"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4369895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1052" mass="116114" checksum="2E8586C83B690F33" modified="2023-11-08" version="1">MPPKRKSKGPKAATHTSLKTPAGNRASAVSNKRRASHSLQGSKMSRLTPSVPFQMTTRRGAKTTSNHASPVGSSSASSDSRRNSMNGIAQFEDAPSDLEDEQPAKRSRISTDSGSPQISNASFDNNNTPVNGAQTPELQMPVPDAISTASRIAGKKRRASDDSSQSSKTLGPRQNGLLTRTQSDISEQQPRRKKRKTREAPADAADQPPELTDASTAPNSPEQIQDVDGSQGLQNVLPTNGDAPTKSGRRLPGRRRQPHPDISVEIDLRRQLNLKMSYRSLAKVQKAVLEEMSTRTTNNLEKDPDYHKQCPEYKPLMASLDQRRDSRLDQINAMRTYRLDQLERVRVAEERIQREQYINRFKELQDDFLLQCYFRMKKVEREMKGVESNATDDEDNVVAPTYTDEPHHNADDRIGSKFASRSRAYVEADKELENDSRRARFNQARAVFVEKEDDADDSIEEVSGGFARYTGPDRTEAILHHNITSLADAAIAVERTPSIIPNEHASLLMMLADVSTQQLRTDRMQETQYHNQQTQLPQPPSSKRATTPMIKQELTKQMSPVFAPPELPSTMPSQAAHSSPINVSQPAVDLGQEASYSDASGVDAMKDVEAAKHTTPVRVSTHRIMDILNDDQEVPVSRLRESQQPAQEPTSPSRREVPTQPNLEPQSDAMRLNAIVDRQEPPVDQALMDALGEPSNAPSNPPSSPPSNTQSWPRSSIAPSSESEDALRRRDPLRKIRALLDRKAREYGREPPDRTQPDYYPRPEYAQFRASLPGNQQRADIAGYDPTRPSAGLYSASPIATSAYPEAARRGSHDQSAPHWEHDRRMSASQVPQQTAASPYQESAPQPYQREHNKPAATPPTHQSPYAPPPGSLPLPPKPPGPPPSGPINFRFAHYDPAPPRQSYPPPSPSYPPGPHGSLGPPPPQYSNQYGGTPAYQGGYVPPLGSFQAPPPPPTSSMPSYPPLKIHQYGGQPILPANMAPPPPSGPPMTFVGQPAPPPAFSPLPSQSLEQEGQRERPPEPQARPRRQYRSYHAPGTQFRSYQGPGERRRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CML4</accession>
    <name>A0A9W9CML4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_005020</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CML4"/>
    <dbReference type="OrthoDB" id="5229455at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF43">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G01850)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00755">
        <property type="entry name" value="AFLATOXINBRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="18"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="271"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4370499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="764" mass="85309" checksum="CC5736ADAF1BB38C" modified="2023-11-08" version="1">MPRPKKPGVEPKRRSRNGCWPCKARKVKCGEEKPHCANCERQGETCDYSIRLNWGGRTKREKIGTGSEDSSAASASPYQSTFSFEEDVLPLFPSTPEPPVHPQHARSHSNISPPTSSNFLIDPELMRMSNSQTSPIVATVGSLDQNSHFVSTSKLPTSAPYTQTHFRSTLDYPPHVVAGSETYNYQDARFTSTNPEMPPPQAAIDPIARRHKDSYSMDSPMAATSDAYSPITVGSDEAVLRAVPKQPMSMYTPPDLRRMSVQSLINGRPGDNSRRYSPEVERGRQYPVADSASITYGYDRGLPDLDTPNNDDFSAIAIVSPQSDIMDLDGDTPYGSAEPRGKDMAFESGGYYAKPVAIRISRALGPLPPLLVENQMNLLYFHHFLNHTARILVPHDCERNPFRQVLPEMAVRDENIMNLLLAYSAAHRARMLNHPEPSNRIAVWVQDVFPKLRQTLIDDPDNISDNTLAAVIMMASLEIICSGTFEVPISWQNHLTMARQLIIARGGPRGMGRQDRVAYFLSRWFAYLDVLGSLSGNKNDTPLGAFYWSSENATADEDFEIDCLMGSTTHCFGSLARISELAKLCESQRIDDDGNVREDWTPNPDVVRQAMHIRRELEEGLLDRNIRKGCNHRSSAASESEGAWDAIEIYATNEMFHWAGLIHLYRRVLGKPAIDPEVQHAVREIVGLFYKIRRGSTAEACLLFPMFTAGCDAQDEGQREKIMDRLRGVEGFGLNHVPKISNLVKSVWDTGKPWESLVSGEFFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMY3</accession>
    <name>A0A9W9CMY3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CHS3_1</name>
        <name evidence="13" type="ORF">N0V83_004051</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMY3"/>
    <dbReference type="OrthoDB" id="370884at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054295">
        <property type="entry name" value="CHS4-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF16">
        <property type="entry name" value="CHITIN SYNTHASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03142">
        <property type="entry name" value="Chitin_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22997">
        <property type="entry name" value="CHS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="484"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1063"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1091"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1118"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin synthase 4-like" evidence="12">
        <location>
            <begin position="392"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="520"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1187"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="175"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="525"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="561"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="587"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1197"/>
            <end position="1219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4372277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1219" mass="137300" checksum="E0936B58CD5D360E" modified="2023-11-08" version="1">MSLPQRPDESSPRRDQRNVFRESPKRRRRTSDLESRRSHDRSTSKSGAKSYSRPLPSPGTPVPMERDPIMRGQHTTPSQELGRKRSLIRPERQRIDPNHPNYHYRKHAQRMAVQPSTTGNDPIMEDRLEAETVSSDGTDLRPPHLRNASGGGYNDKQAPLDDEPPRVHRRLTRSKTMENLEKQRQKEKDKMRPPSLWNVYCAIVTFWCPDAVLKCFGKPQKAQQRAWREKVGLVSIILLICTFVGYLTFGFTETVCPSGGNARLRVNKVDLGHLIVHGKAYDLTQSTHPLARGVPAGANVLFDLPEKHGGQDASFLFQNVNGACKGLITAKDNSGIPTDGSGDLAWYFPCRLTNQDGSTKPNMTFLEYNGYMCHTSDNARRAFYGLRDSGDVYFTWDDIRNSSRNLMVWGGDVLDLNLLDWFNRTQVNVPTIFDQIRQNPAVRGIDVTRSYSSSYEKQVARCFTEIIKVGSIDTESIGCIASKVVLYVSLVFILAVVMAKFVLALTFQWFISRKFGASKTSVGKTDSKQRKQQIEEWSDDIYRPPPRLADPASGSDRSSKRGSTFLPSTSRFTSPYTMEKSAKTRAPPTTMTSQSTASRLLGSNSGMYRQLNASHGTLPTYDGKHSHQESRASLLLSGTTDQNRFSTAMGESEGPGPAGFIHEAVVPQPPPEWQPFGYPLAHTICLVTAYSEGAEGIRTTLDSIVTTDYPNSHKLILVICDGMIKGKGEDLSTPEICLGMMKDHAVLPHEVQAFSYVAVASGSKRHNMAKIYSGFYDYGEDSRIATDKQQRVPMMVVVKCGTPDEAKKSKPGNRGKRDSQIILMSFLQKVMFDERMTELEFEMFNGIWKITGISPDFYEIVLMVDADTKVFPDSLTHMISAMVKDPEIMGLCGETKIANKRQSWVSMIQVFEYFISHHLAKSFESVFGGVTCLPGCFCMYRIKAPKGGQNYWVPILANPDVVEHYSENVVDTLHKKNLLLLGEDRYLSTLMLKTFPKRKQVFVPQAVCKTTVPDEFKVLLSQRRRWINSTVHNLMELVLVRDLCGTFCFSMQFVVFIELIGTLVLPAAIAFTFYLIAISIKAAILHTAAPLIPLILLALILGLPAILIVVTAHRWSYVAWMFVYLLSLPVWNFVLPTYAFWKFDDFSWGDTRKTAGEKTKKAGLEYEGEFDSSKITMRRWHDFEAERRLKTPGGGWSQQPTSAGGWPQQQQGYEQYYDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN00</accession>
    <name>A0A9W9CN00_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005246</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN00"/>
    <dbReference type="OrthoDB" id="2157530at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF73">
        <property type="entry name" value="HET DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G01020)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26639">
        <property type="entry name" value="Het-6_barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="61"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="506" mass="57630" checksum="AED3D439984D2607" modified="2023-11-08" version="1">MEGQRSTYTYSPLNEAHREIRLLHLLPKQRKTTHSGVEHARELEDIRCTFSLASLNDHPPFEALSYVWGDPKAVIPISLHGHEFPVTLNLHDALSNLRLDCEERILWADALCINQADNDVVFQFGQRARDASIIRDFADDMYRHAGECCRCTPYLRFENSSGVSVSGGIARFKRLTKSHENKDGLDLLSLMGGNSTRECYDPRDKIFGLLGLCGYPGDYRPDYSKSVRQVYEDFVVSQIREAQSLDILSYGYGARHRNLRLPSFVPDWTATLPHDSSLDAKQRYKIVRMGTFNAADCSKEEINIFHPGMGVTRAIAVDTIVDFDHLHGARSLKQEKILERHRRLAKVDDRTQVPYTSRIAALWKTWWGGITLQMNNARVTVSRIDDLDGSNYQQWQKGLFWDVSQKNVDPFYKIAFNYVSAGRKFIVTKMGYMGFAPESCEKGDLIVLMPGGKVPYILRPASEADEIPFTDWTPGNRFTFLGDAYLQGIMHGEAYDEERLELIALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN03</accession>
    <name>A0A9W9CN03_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004527</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN03"/>
    <dbReference type="OrthoDB" id="7042322at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050478">
        <property type="entry name" value="Ethylene_sulfur-biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795:SF39">
        <property type="entry name" value="AMINOTRANSFERASE CLASS I_CLASSII DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795">
        <property type="entry name" value="BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00753">
        <property type="entry name" value="ACCSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="2">
        <location>
            <begin position="33"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="316" mass="35117" checksum="5F073E2E3D7C2557" modified="2023-11-08" version="1">MSAVRAGGVLIPAPFKSVEGYRGLDDVFDPDMNRKALEKTLLQATQDGVTVRAVLISNPHNPLGRCYPAETVREIARFCGRNNLHLISDEIFALSVYQNEQATNVTPFVSVLGAGLDECIDSHLVHVMYGMGKDFCATGLRLGVLHSTNEGLIAAVSSISVFGWVPYVVQDVWADMLEDEQFLGDFKLENRKVLAKHFTILRSFLDEHNIPYYSNVHAGVFVWVDLRRYLHANSAPEPATSAVDVNHDQEVKLFKRFLRAGVVVSQGSGFGTEELGWYRISFAVEEQALNVGIQRLGTCLKSIENDGWRIESCRSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN98</accession>
    <name>A0A9W9CN98_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_003190</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN98"/>
    <dbReference type="OrthoDB" id="9986881at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF31">
        <property type="entry name" value="POLYAMINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01036">
        <property type="entry name" value="TCRTETB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="334"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="369"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="442"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="480"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="519"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="546"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="578"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="615"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="211"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="33"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="137"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="652"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="33"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="654"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="683"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4372899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="698" mass="77440" checksum="236BE5CD3E0560A3" modified="2023-11-08" version="1">MQEEDYEILRNEANASPERYAEWDRHRQFREEYAERHRNEPGYDERIRQEAIDREGQPGTKEEEVAPLSRHSSVSSASTSTSASSHSTHSERLEEIRTVRSTGRNRGDTVTSRASTGNILQLHPTERDPEAIRRIETHRSQHRSTVGASDGESRLTRTLTRRKTERPLPNLGAGKPFPPPLPEREEYVVEFDGVDDPLHAQNWPMNKKLGIGAILAFDALAATMGSSIFSSATRVVSQEFGVANVVGTLGTSFFVFGYAFGPLAWAPCSELYGRKPPLIIAAFGFAIFSIAVAVAKDLQTVLICRFFAGLFGSSPLAIVAAVFADMFNNKLRGLAVAVFSATVFMGPLLAPFIGGFITESSLGWRWTEYISSFMGFVALALLLLFMEETYPPVVLINKASELRRRTKNWGIHAKQEEIEVDFKELIVRNVSRPMRILFTEPIVLLITIYMSFIYGLLYLFLTAYALVFQGVYGWSSGVSGLAYFGMVLGELIAFVVIVLDNPRYARKLEANNNIPVPEWRLPISMVGGVLFAGGLFWFGWTGYTGNIHWIVPILSGLFTGFGIFSIFLSLLNYIVDAYLMFAASAIAANTFMRSIFGGVFPLFATFMFEGMGIEWAATLLGCVAVVLVPMPVIFYVFGKRIRARSKFAPAPDIEQDKRRDEEARLGSGNVAGDVGSGESTDGDANKERGLQKEKSKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNF2</accession>
    <name>A0A9W9CNF2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed RNA polymerase III subunit RPC9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_003355</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPC9 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNF2"/>
    <dbReference type="OrthoDB" id="1746530at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038324">
        <property type="entry name" value="Rpb4/RPC9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038846">
        <property type="entry name" value="RPC9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15561">
        <property type="entry name" value="CALCITONIN GENE-RELATED PEPTIDE-RECEPTOR COMPONENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15561:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="144"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="165"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4373064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="177" mass="19846" checksum="76C057C33733023B" modified="2023-11-08" version="1">MHIVNPQSALLSNHELLLHLQQEEAEYSGTDGTGRNRPKPSGLNHMLRDGIAYLQSADLPNGAIASTHPDRPMTLYKGPHSLFRALSPKYRLNKAEYLQLYNLRPTTQVMLELVIEEAGSRFSEEQLHDILSIIQQVFDEEEASIPAGAEDQEMPKIPNKLLGASRKKRKPRAKAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNN0</accession>
    <name>A0A9W9CNN0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003613</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNN0"/>
    <dbReference type="OrthoDB" id="4419870at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4373319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="259" mass="30239" checksum="C4CEC77D7520CF0A" modified="2023-11-08" version="1">MCFRCERRDHFRGECAEKICYGNPRFDKLPTTLEEAVVQRDAWVTRYVIMFDMIKGRVPEEAAGPLRAGAPHLPFDERPETLDEAIRSREVSKAHCMAMFNTIQSHGFKAELDPRIRWDIDPASHAIDTYAQSIEEGITNMNLRQLSVSMSRIHDHIEALGWMQGKSLYWRYTLALLLMHQTSYWVIQLSIYPDENREEIAALKEVNLALDKQLEGILREGQKYLPLNQARITLNASQAEEFYPRCWEFLEAQQFSWEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNR3</accession>
    <name>A0A9W9CNR3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004053</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNR3"/>
    <dbReference type="OrthoDB" id="8119704at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF33">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G14860)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="30"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="208" mass="22928" checksum="06EB032E2B304F68" modified="2023-11-08" version="1">MDTHQHQHQHHLPTSPPEIWHTTTGTPGSPTLLLLHGLFSSHLEFSALLPHLTPYYFILLVDLPGHSNSQSATLNTTTTYRFPEITSALATLIRTSSPSGTAHLAGLSYGGFLALSLARAHPELVASVFTSGSAPWAGIQRFVATRPRLLWCVIAPPILYVPDSLFAWLLKQMGLADLEAMREESKRNFSNELMRAGFEDCAGSGWRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNX1</accession>
    <name>A0A9W9CNX1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002509</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNX1"/>
    <dbReference type="OrthoDB" id="5376590at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070224">
        <property type="term" value="F:sulfide:quinone oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070221">
        <property type="term" value="P:sulfide oxidation, using sulfide:quinone oxidoreductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000203">
        <property type="entry name" value="Related to sulfide:quinone oxidoreductase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015904">
        <property type="entry name" value="Sulphide_quinone_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10632">
        <property type="entry name" value="SULFIDE:QUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10632:SF2">
        <property type="entry name" value="SULFIDE:QUINONE OXIDOREDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="25"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="432" mass="47216" checksum="4C85E14AC3EB16FE" modified="2023-11-08" version="1">MRPAIQGVRGLATVAPVNSVTRNHKVVVIGGGSAGIALSHQLLRKGNFTQDDIAIVDPAQWHHYQPGWTLVGGGLKDKEELRKPLNSLINPKFKFYNKKAGVVSPKENLVSLEDGDKISYQHLVVAPGVKVDIDTVKGLSEALANEDALVSTIYDYNYCDKAFRTIKKFDKGEAIFTQPAGVVKCAGAPQKIMWLALDYWKRAGLYNSSSSSPIGMTFATGLPTMFGVPKYAAKLEELRVARGVEGLFEHDLVAIDGQTATFASPGKEPVKKHFDFLHVSPKNKPYAWIKESGLANEAGYVDVDDGTTRHKKYSNVWSIGDASSLPTSKTVAAITSQAPVLVSNLLQAYEGKEPTSVYDGYTSCPLLTGDSKVLLAEFKYGGVPKETFNSLFGIDQGVPRRAFYHLKKDFFPWVYGRYHVNGTWGGPKGLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNZ7</accession>
    <name>A0A9W9CNZ7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Something about silencing protein 4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004294</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNZ7"/>
    <dbReference type="OrthoDB" id="1938992at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033255">
        <property type="term" value="C:SAS acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038988">
        <property type="entry name" value="Sas4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029184">
        <property type="entry name" value="Sas4_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38422">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38422:SF1">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15460">
        <property type="entry name" value="SAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Something about silencing protein 4" evidence="2">
        <location>
            <begin position="85"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="284" mass="32250" checksum="EC727555210CFCE6" modified="2023-11-08" version="1">MDVFLHVDTPIYLVDEPTKSKTPAPVTPEPSTKPVSPSRLRKSKDSSPPRGASNPATSPTQSSTSFQVLDYATVAKGLRYADGEDPLADSVYFTQHRRAERKEKQLRNIEKERAMHEKVQLERLLDGLQGPDWLKVMGITGVTDGERKDWEPKRDYFVKEVEALVDKFRIWKEEEKRLRAEKEAALAARDEDEEEDGEDTEASDTIRLKTRDAPPPAPPRHHAKEPSDPPKPRRPPRPHGFLLPPIPRTRRPLPILLLQTTSPRRGPGKASPRPQRERLWAADP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP92</accession>
    <name>A0A9W9CP92_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_003401</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP92"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000078">
        <property type="entry name" value="MFS maltose transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF5">
        <property type="entry name" value="ALPHA-GLUCOSIDES PERMEASE MPH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="232"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="359"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="442"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="485"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="514"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4373110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="573" mass="64186" checksum="1CB8B3B0122338F3" modified="2023-11-08" version="1">MASVNEVKLNRIQKLLKTLEQRDKPDETSPGSTKNDTLVACTTARSTISCLTETPSFVTEKSMTLRQGIRLYPKAIVWSVHLSMTLVMEGYTTILIPNLFALEPFRQQFGTPQADGKYEINAGWQSALVNGGLVGQIFGLFAAGTLSEKIGYRRTLMVGLVAISLAIFVPYYAKTKVVLLAVYAADVCPVALRAYLTTYVNACWVLGQLMAAIVLRGMISNTTSVWSYRIPFALQWIFPVPISIAVWYAPESPYWLVRQDRIPDARYALHRLRKRSSNETEEEHHYWTCDTIEKMVETDRKEKKLQSGTRYWDCFKGIDRRRTEIACMCWTIQTLCGGSIMGFSTYFFEQAGLSSRHAFTLSLGQFALGLVGVIISWALMVYFGRRTLYLSGQALMFMILLLIGILACVPQKHTTIKASPAPGAIASSASKTANVHPSALNWVIASLLLIFTLTYDATVGPICYSLVSEIPSTRLRSKTIVLPRNSYNISGIITLFITPRMLNSTAWGWGAKAGFVWAATSLVGIAWSWWRLPEPNGRTYDDLDELFERKIPARKFRTTGLLEKSTEQEGRQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ12</accession>
    <name>A0A9W9CQ12_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MEP1</name>
        <name evidence="11" type="ORF">N0V83_002421</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ12"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000003">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF4">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="309"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="392"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="10">
        <location>
            <begin position="39"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="459"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="460"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="487"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4375335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="518" mass="56071" checksum="2963F1395FE64B41" modified="2023-11-08" version="1">MSYSWTGAPAEFNGTDSTTGGDSVTENLNQWYQSGDQAYILVASAMVLTMVPGLAFLYSGLARRKSALSLIWACMFSCSVITFQWYFWGYSLAFGPGTSGFIGNLARFGLKGVLGAPSQGSPLIPELLYAFYQLQFCAVTAAIVVGCVAERGRLIPMMIFIWLWATIVYCPVAYWAWNANGWFFAWGGLDYAGGGPVEIVSGCSALAYSMILGRRQEKMMLNFRPHNVSLITLGTVFLWFGWLGFNGGSAFGANLRAAMACWNSNLTAMFAAMTWVLLDWRLARKWSMVGWCSGTISGLVAATPASGFIPPWASVVLGITTGIVANFSTKIKFWIKIDDSLDVFAEHGIAGMVGLFFNGIFGADYIIGLDGVNNGLTGGAITGTGSTVYKQIAYIVAVTAYSFTVSAILAFVIDKIPGLKLRASEEAELLGMDDDQLGEFAYDYVEVRRDYLAWTPAKNEPIDGEHSVPQGERHGIPQHSQMLEGKAPSEDSGHEHTGIAGDRHAVAMGTDQEKQAQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ51</accession>
    <name>A0A9W9CQ51_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">homoisocitrate dehydrogenase</fullName>
            <ecNumber evidence="12">1.1.1.87</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LYS12</name>
        <name evidence="14" type="ORF">N0V83_003084</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(2R,3S)-homoisocitrate + NAD(+) = 2-oxoadipate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:11900"/>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57499"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.87"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-alpha-aminoadipate from 2-oxoglutarate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.87" evidence="12"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ51"/>
    <dbReference type="OrthoDB" id="10261637at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047046">
        <property type="term" value="F:homoisocitrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009085">
        <property type="term" value="P:lysine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000012">
        <property type="entry name" value="Homoisocitrate dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF48">
        <property type="entry name" value="HOMOISOCITRATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="13">
        <location>
            <begin position="7"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4374343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="357" mass="38301" checksum="95410EDA10069408" modified="2023-11-08" version="1">MAARTLRIGLIPGDGIGREVIPAGRRILEALPSSFGLKFSFSHHEAGWETFQKTGAALPEKTVEALKAECDGALFGAVSSPTVQTKGYSSPIVALRKRLALYANVRPVKSVASAKNPVDLVIVRENTEDLYVKEEKTYNAPDGSKIAEAIKRISETASFNIAAMAGDIALRRQRVREAGADSIHNSPLVTITHKSNVLSQTDGLFRETARRALSDSKYQMVNVEEQIVDSMVYKLFRQPQDYDVIVAPNLYGDILSDGAAALVGSLGLVPSANVGNDFVIGEPCHGSAPDIEGKGIANPIATIRSTALMLEFIGHEDAAAKIYAAVDANLEEGRLLSPDLGGSAKTEEIIEDICKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQD3</accession>
    <name>A0A9W9CQD3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin gamma chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Gamma-tubulin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TUB4</name>
        <name evidence="12" type="ORF">N0V83_001413</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Spindle pole body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQD3"/>
    <dbReference type="OrthoDB" id="10249382at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02188">
        <property type="entry name" value="gamma_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08570">
        <property type="entry name" value="GDPD_YPL206cp_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000004">
        <property type="entry name" value="Tubulin gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000003">
        <property type="entry name" value="Tubulin gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000012">
        <property type="entry name" value="Tubulin gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000073">
        <property type="entry name" value="WGS project CABT00000000 data, contig 2.28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002454">
        <property type="entry name" value="Gamma_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01164">
        <property type="entry name" value="GAMMATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="11">
        <location>
            <begin position="65"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="808"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4376132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="830" mass="93234" checksum="1946841511F30BF0" modified="2023-11-08" version="1">MEADSSFDVQTPILENMVVGVPPVFEGLTKQLSKIVEQPLLGPKANFPAASFAFGRKDVSGNRRPQAIAHRGYKAKFPENTMGAFKGAVEVGAEGLETDIHLSKDGVVVLSHDKDLKRCFGRQEKIIDCDYEFLSTLRTLKEPHEAMPRLADLLEYLAQPGLEDIWVLLDIKLDNNADDVIRLIGDTIRSVRPSPSRPWHSRIVLGCWAAKYLPLCSHYLPGFPISHIGFSTLIASYFFTVPNVSFNMQQAILMTPWGKLFIRKAQREQRPVYAWTVNEESRMRWDIRQGLDGVVTDDPKLFLEVRRGWHEGTKDAVSIMTWLDVLRMNFFALIFSVLFRIMFGFSEGESLVKMKPAAEEGDPPAPPVHNTMPREIITLQAGQCGNSVGQQFWQQLCQEHGINQDGNLEDFATEGGDRKDVFFYQSDDTRYIPRAILLDLEPRVLNSIQASAYKNIYNPENFYIHKDGTGAGNNWGMGYSMGEQVHEDILDMIDREADGSDSLEGFMMLHSIAGGTGSGLGSYMLERLNDRFPKKLIQTYSVFPNTQDGDIVVQPYNSLLSMRRLTQNADSVVVLDNGALSKIAADRLHVMNPSFEQTNQLVSTVMSASTTTLRYPGYMHNDLVGIVASLIPTPRCHFLMTSYTPFSGENVEQAKTVRKTTVLDVMRRLLQPKNRMVSTNPTKKSCYMSILNIIQGEADPSDVHKSLMRIRERRLATFIPWGPASIQVALTKKSPYVTSSHRVSGLMLANHTGIATLFKRIVAQYSTLRKRNAFLESYKREAPFKDGLGEFDEAKEVVQGLIEEYEEAEGADYLSKETPPGDEAEDKRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQP2</accession>
    <name>A0A9W9CQP2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Poly(A) RNA polymerase mitochondrial-like central palm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQP2"/>
    <dbReference type="OrthoDB" id="273917at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010605">
        <property type="term" value="P:negative regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF15">
        <property type="entry name" value="INACTIVE NON-CANONICAL POLY(A) RNA POLYMERASE PROTEIN TRF4-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="2">
        <location>
            <begin position="349"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4376446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="521" mass="57849" checksum="8D906AB587A2081C" modified="2023-11-08" version="1">MDRMTFTSGGGDSYRPGVPQRQQGRADDRSQFSFESNHPAPQFPPSAPTGPANARPREPPRKRTRRGALQHGSQRGDASRSQGRNNANGFRKNFRKAPHERALLTHRDDGGPEHAMGVSDGPNKFLNLEDLSDDEEADMEVEGDASDNNDTDTVGQDVNHKVAKRQTTSRADGDSVPKWSNPDPYTVLPPPEETTGKIPDFIKLIRKAKNTAAEISAGDTAVAANDDFISFGDEETFREPSEVRSLEYEEPDSTRMRPNAAAKRSVQGSLNELDAVVSLPQSTQEAPGPSNRNSHPARDPARANKRKHGGFLGIVQEWLPKPNSNPTPWVHSDGQYDRIRSEPDTTKWLHNEILDFYDFVSPQQVEHDIRNHLVQRVNSALGNRRFPNENGRILCFGSFPAGLYLPTADMDLVYTSDTHLNGGPQILDPSQKGAYKNLLYKASRRLQDMRISRGRCNVIANAKVPIIKFTDDLTGLEVDISFENLSGVRAQVTFADWKQQYGDLVSGILALTKFSSPVVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRS4</accession>
    <name>A0A9W9CRS4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRS4"/>
    <dbReference type="OrthoDB" id="4500473at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="290" mass="32304" checksum="AB4AFD5476991804" modified="2023-11-08" version="1">MSMPAYVPTYFLARTRNNPPDGLIKLGNLIISPELPDEPIELAKPLPITNGTTHDEEGWKYDFGKKTDVSNSIGASFLESLLGAGGDAGVSWLRENSQEWSCEKMITYEFQPTEAWLKDLVADAKVRERLNKMKFWKRSGSFYVVVGVKVAIAASLVAKFARGVGLHLHASVDLTPATTIPISAHTDQDVMRERHRHETVGKIDDFVIGYRLRRLKISRTGDIKKNQQHNDGAAFRQDDGRIDNTQNADISVDELEAHDVQGWELKMETTIMTESEGSTKYACVKTGLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CS24</accession>
    <name>A0A9W9CS24_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glucosamine-6-phosphate isomerase</fullName>
            <ecNumber evidence="6">3.5.99.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Glucosamine-6-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NAG1_1</name>
        <name evidence="9" type="ORF">N0V83_000490</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the reversible conversion of alpha-D-glucosamine 6-phosphate (GlcN-6P) into beta-D-fructose 6-phosphate (Fru-6P) and ammonium ion, a regulatory reaction step in de novo uridine diphosphate-N-acetyl-alpha-D-glucosamine (UDP-GlcNAc) biosynthesis via hexosamine pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>alpha-D-glucosamine 6-phosphate + H2O = beta-D-fructose 6-phosphate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:12172"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:75989"/>
            <dbReference type="EC" id="3.5.99.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.99.6" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CS24"/>
    <dbReference type="OrthoDB" id="7663298at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004342">
        <property type="term" value="F:glucosamine-6-phosphate deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006043">
        <property type="term" value="P:glucosamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006046">
        <property type="term" value="P:N-acetylglucosamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019262">
        <property type="term" value="P:N-acetylneuraminate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01399">
        <property type="entry name" value="GlcN6P_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000002">
        <property type="entry name" value="Glucosamine-6-phosphate deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004547">
        <property type="entry name" value="Glucosamine6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018321">
        <property type="entry name" value="Glucosamine6P_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00502">
        <property type="entry name" value="nagB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11280">
        <property type="entry name" value="GLUCOSAMINE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11280:SF5">
        <property type="entry name" value="GLUCOSAMINE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01161">
        <property type="entry name" value="GLC_GALNAC_ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="8">
        <location>
            <begin position="25"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="312"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4377662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="336" mass="37623" checksum="B8ECF87D4BF0843E" modified="2023-11-08" version="1">MYAHAMTTNRKLRPKRIKAFNPTSERPFVLGLPTGSSPVLIYQHLVQRHKAGEISFRNVVTFNMDEYVGIPRDHPESYHSFMYKHFFSQVDVKPDNINILNGNAPDLELECQEYEEKIKRAGGIELFLGGIGPDGHIAFNEPGSSLRSRTRVKTLAYDTILANSRFFGNNLDQVPRMALTVGVQTVLDAREVVIIITGAHKALALQRCIEGGVNHMWTLSSLQMHPHPMIVVDEDATLELQVKTVKYFKSIEMVATELGFRQKLPRKRDSLVDLLAPEPQTNGIKLTVPPVDVARSASPLLEPMSARLSDEEAKILRPMDELEDSPSTRMGARVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSB5</accession>
    <name>A0A9W9CSB5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000793</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSB5"/>
    <dbReference type="OrthoDB" id="3800150at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="297"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="465"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="592"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="85"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="305"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="518"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="563"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="620" mass="69278" checksum="003CFEC0426739CB" modified="2023-11-08" version="1">MEELERLSSPDFTTADSPEAASLLQSSQEDDSQQSEMSCDQSSPVEERLTTKDEGDEPVTTAQSKDNQIRLESSDDDVPIMARRQLRETAKKRTDENAQRERRPVRDAGRQSGDHESNDRPRSYPVSRPTHTIAGKPRKKSGPRAWAPKPIKKNDMVAKKAVIREIESHWGKGFITSFIPKVHRPLVKRGTLGKRSHYRQHETDPKKWLPSILKAILMIAKLTTDKKWLKDAMNEVVRYRIKHTGNRKPQLVTTDFDVIEDMLVKDWPVGYSFKIRYKHLLVNRKDQEKDTDEDIDHILHSGSDQGEDSGGEVDEEDVDDDMGDHEEDDEDEDEDDDDMDQGQGGFSGRYLQSSGYTKGSPYSPPTLPRQQAKQPKPSKPGSTKAAHPRQPLPPGPQQQQNMYGYGGQMPGYPPMTDPWGRPIHFPGGGPEGYNAYGGYVPYGGHTGYGGYGPPPHQQYPIKQERQSAIPPPPHGPYQSRHAMTPALSIEDSDYRGGGRSGNNRSRRTRDGGYEQNIPMPPPMGPPGMPGYPNMGSYEQRGPRPRIKRESPPSAAGDEHGGVVEEDFDGGPENDAYTQDEADDDDDDDDSAAIDAEVEAAELELRLARLRARQAAMKKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XX20</accession>
    <name>A0A9W8XX20_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_010863</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XX20"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4361922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="83" mass="9300" checksum="B6249F490FB40984" modified="2023-11-08" version="1">MFLQRIRDANRGLWPAKQSSIDLPVVNLGNVNRKRKSESMDIDLDMDQVAGRVGDEPVGTDMDIVQTARNMGFELLEIPDSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XX34</accession>
    <name>A0A9W8XX34_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein-lysine N-methyltransferase EFM4</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Elongation factor methyltransferase 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">EFM4</name>
        <name evidence="8" type="ORF">N0V83_010718</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that mono- and dimethylates elongation factor 1-alpha at 'Lys-316'. May play a role in intracellular transport.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. EFM4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XX34"/>
    <dbReference type="OrthoDB" id="10069295at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03188">
        <property type="entry name" value="Methyltr_EFM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026635">
        <property type="entry name" value="Efm4/METTL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12843:SF5">
        <property type="entry name" value="EEF1A LYSINE METHYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12843">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE METTL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Methyltransferase" evidence="7">
        <location>
            <begin position="67"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4361778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="256" mass="28169" checksum="43A19AD5970A02A0" modified="2023-11-08" version="1">MTPPPTHLDPSELGQKSYWDAAYTTERSNFAANAADEGTIWFSDAGAEERMLLFLEDLADEGETHNKSETSFLDLGTGNGHLLFALREEGWMGSMVGVDYSAQSVQLASAIRDTKAKEEASDDQTSDETSSTTAHFDDIAFYEWDILTQPPDATWLVNSGSGFDVVLDKGTFDAICLSQDTDAQGRRICEAYREKVEPLVKKGGRFLITSCNWTEEELRGWFGGGELVFEGKVKYPSFTFGGKTGSSVVTLCFKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXL0</accession>
    <name>A0A9W8XXL0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Geranylgeranyl pyrophosphate synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXL0"/>
    <dbReference type="OrthoDB" id="420564at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35179:SF1">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35179">
        <property type="entry name" value="PROTEIN CBG02620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="413" mass="47128" checksum="720C1A1F46E2D21F" modified="2023-11-08" version="1">MEIPPAEPETPRTTPPVEEEQVEQLREEPRRQHFKDPSWMTRNIRKDNRIALLLHGDVKASSAPVTTTDGYAFLASYSWKQSTAPTIYVPGTPPKWSPPPLPTQLEKDQEGHWVDQHGHRTPRFQFEPIFQALTIMNPNVAFDNVDIVVNRNSLLKLYSFASFKRHPQFYLDLDMIGKTLFISRRESRAKTHIQDGYGGTFENKFTSKDPELADAEGHHRIIQYRLGRLNIVVRIEADAYFSDEHAENSNTNTPNEVFLNFLREMQPASAAIAHTRHIQTNVIPKGTLMPHHRTAELKSNNDKRGATTQAWFGRTRNLIFGGNKKGLITSTSTKQLQDKDFADWAEKNQLHLRRLAWLLEEMRTAVKEKTKGAAVLVATEKGAPLQIFEAFQSFGALPKPVIKRFWSEDVAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYE0</accession>
    <name>A0A9W8XYE0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pyruvate decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010047</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYE0"/>
    <dbReference type="OrthoDB" id="10006023at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF166">
        <property type="entry name" value="2-HYDROXYACYL-COA LYASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="7">
        <location>
            <begin position="18"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="5">
        <location>
            <begin position="208"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="6">
        <location>
            <begin position="410"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4362930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="624" mass="65979" checksum="B6D938F31E63B470" modified="2023-11-08" version="1">MSSNKLNLKQPKGRELLGGDLLAQCLYQLGVKTIFGLHGGHLDAFLMGCTDMDMELIDTRHETVAVQAAEGYAKVSGKTGCCFVTANSGFCNGLPGLATAFADRSPILCITSSPPLRDAETNALQGFHDQVVLAKPVTKFAHRFTTVQEIPRIVSLAWRATSAGAPGPVLLDFPIDILFTPVDEIERVSWGNITGPPVSLPGPDPNAVKELVGLIKAAERPAVIVGTGARGAVDEIANLASAINLPIFHSPKFSTGIPSSHPLHAGSALQLALLSATQKPHPDLIILLGARTGFLLGGRGGAVVPTSNCKIVQIDVDGSEIGRSEHVDLGIISDIKLAIAALTTQLQSSTEKQFKVAESWTKTALGLKSWRAPHNESEPVIDKENGHLHPYHAVKAIYRALPASSIICIDGGEAGGWALQLLPEAPSPALAMVTTGYLGFLGNGWGYSLGAAVADRSKLVVNIQGDGSAGFHLAELDTYKKFGLKVLTVVVNNCVWGMSQAGQEMIYGHLTDKKPASALRKETRYDVVAEGFGMTGVLVDTTTATKEKGKEGEKGALEAVGKAVEEVVKSGGPGLIDLRVSPHPYQDSTKAMVGATKDPDVIVVPYYDNLPRPYYKTKKVNGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYJ1</accession>
    <name>A0A9W8XYJ1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010269</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYJ1"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040849950" evidence="2">
        <location>
            <begin position="32"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="94" mass="9841" checksum="1B0A1658F184F073" modified="2023-11-08" version="1">MALKALKTPSWLAVIFVSFILATHLPTACLAQDKPVSSNGNPTATADAMNPDPSSSTTSSAPTQTHTIQVGLADHVFKPDVTNASIGDTIEFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYN9</accession>
    <name>A0A9W8XYN9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA (cytosine-5)-methyltransferase 1 replication foci domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYN9"/>
    <dbReference type="OrthoDB" id="5382953at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="164"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="396"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="582" mass="64858" checksum="1C20038E1874B14E" modified="2023-11-08" version="1">MPVPETDVLKPVDPSITDSDHWEIFVLSDAQVFYESNGKPASLLAAYADTPLRVQGRLDAPGRGQMKYFLTKPYKPVDIEIRHVTRFSYGEMTDGAYVIWAQGKAGWFEIRPAQRYQAVFDDMARAVELLYFVTDIYNEPRKRGGGPSAQLIFQETVQQKRTSKSSPAAVPASASVVTAAKSRRSQEKTATPKSDEPPKKDENWWEAAALFEFMQKAVNQRVLRAGRNHITLKRVAELVVRRYEIEELETAQNVLLVHARNLCYMMDHPRRKAVSFFANEPIYQELATGHSLSAAEQRRAESVELRPRRDHATLRHGESESSDTSDEEEDDDAITTPVRRPPGRRKKGRLSVLRPKSSKFSGKGKGKGIKGRTGKAGKGKAPVPDSEAPSDDSEAEGSASDSGSADEMGIDTPTQVLSPGREKRKLVETDADREEEKGRRKRAASASIGPTSPPTSTESGDEDEQAASDEPPLPLRHRPSNVPPHSRPLPNTKPATVPSIVSTPLPTYEPNGPRDSWICSFDGCSQRIYGCSKEIGRQLITEHLEDHSKGREKVVGILWREQDKLHLPVRLVEIKMRRLNKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYQ3</accession>
    <name>A0A9W8XYQ3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010541</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYQ3"/>
    <dbReference type="OrthoDB" id="2958217at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112:SF12">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="109"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="181" mass="20755" checksum="6F6C9EDC02350643" modified="2023-11-08" version="1">MEEKLRSNEIEVPQSGQVEARLNLDVSDFRICQICDDDLPIQSQWLRERRVTASKLDVSLFKNWLQTCCTKHGKHCEKPTWLSPKIPENLRVIDIRTRSVIKAQPDCIYAALSYVWGGPQYIINDAQMRKLPFCLPEPPVLSQTLVDAMALCHQIGIGYLWVDALCITQEPLVNDDKSFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZR3</accession>
    <name>A0A9W8XZR3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MICOS complex subunit MIC10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MIC10</name>
        <name evidence="10" type="ORF">N0V83_010604</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Mitochondrion inner membrane</location>
            <topology evidence="2 9">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the MICOS complex subunit Mic10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZR3"/>
    <dbReference type="OrthoDB" id="1916310at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007512">
        <property type="entry name" value="Mic10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21304">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21304:SF0">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04418">
        <property type="entry name" value="DUF543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4362510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="96" mass="10645" checksum="5A90F975A63C29F7" modified="2023-11-08" version="1">MADETPKPVAPIPRTTRPMSEALLNEKWDRCLSSMLIRSGLGVSFGIVFSVLLFKRRAWPAWVGLGFGAGRAWEECDNSFKRAAAPSKDGLRVIRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZX1</accession>
    <name>A0A9W8XZX1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009999</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZX1"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="316"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="427" mass="46125" checksum="F9DF2D75632E7118" modified="2023-11-08" version="1">MAPGTSDAHSSSAPATQGLREQLSVMYHGFETLPETSPDDHDADDEEDEREVKVTRTTNKANDANRDNTSSSTNPNPTNGESETKPKNVDKTRTDALDGLTRYFDFPPWGRGKKKYPKSSPSLIPLKRRGPFIGFTRSFTTDDSGSDGHGPGRKKKKPAPRRKPQSPPLRKKNPLPTLGMLPECPIEIEEDESEELQKPGSSLAAAAAGGQEPRSTSSITASQDADAAAINYHIAALPTCAVKDASTAEKCASREKSGDEDVSSDDSELLIYEHGGRLVRFGHHTHNHGRKALDDVPDASAKTPSPTPPPPREPSRTTTTTTLSSPSPSSAAPAHLHLQLQRPPPPTSPSPVILSAARMRIFERTFRAIVFADFTSITHVMASVNGWVNKAAGEYGFSLEEARAALGVLHERKVVRVEGDTVFFNNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0B5</accession>
    <name>A0A9W8Y0B5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009973</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0B5"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="196" mass="21981" checksum="0C7F8A04F4CC37C2" modified="2023-11-08" version="1">MDQSVHNAYCASKAQCMTYLNDLRLSKTTPFSIVQVIPGTVIGPSEFATTPSAAIAHMDRQTKALLFDEMKPRYAFGFVHVQDCARVHVEALDEAKAKSEELPPWFVAAATIEEGVDGARLWGTVADMVEREYKEEVEEKVFTVGRSKVPVNMPYRVDARLTERMLLGGEKIRGLEESVREVAQWYVDLKKKGKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0F2</accession>
    <name>A0A9W8Y0F2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_010272</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NmrA-type oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0F2"/>
    <dbReference type="OrthoDB" id="9997102at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051164">
        <property type="entry name" value="NmrA-like_oxidored"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42748">
        <property type="entry name" value="NITROGEN METABOLITE REPRESSION PROTEIN NMRA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42748:SF7">
        <property type="entry name" value="NMRA LIKE REDOX SENSOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="3">
        <location>
            <begin position="6"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4363152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="302" mass="33569" checksum="A83914095A44FBC8" modified="2023-11-08" version="1">MAAARAILVTGATGKQGGSLVDALLKRNTSFEILALTRDAQSTSSQKLLKKSPSKIKLVTGNLDAVDDVFRKAKEATKAPIWGVFSAVGKNEEKQGIALVDASLKNGVQHFVYSSADRGGTKSDTDPTNVPHFITKFNIEQHLFAKAKGSNMTWTVLRPVAFFDNLTPGFFGKVFSTSFVMNLRESQKLQMIATSDIGFFAAESFWNPQSERYRNKSMALGGDELTFGEFKKVFEQTTGETLPTTYRFLNGILLWMVKDLGHMFTWFREVGFGADMQGLKKVNPGMKDFKTWLETESAWKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0J0</accession>
    <name>A0A9W8Y0J0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010681</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0J0"/>
    <dbReference type="OrthoDB" id="3794114at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040869773" evidence="1">
        <location>
            <begin position="25"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="256" mass="29036" checksum="5417D01395605202" modified="2023-11-08" version="1">MYFKYTTVLASLLALSQTLWTPQAVPVTSLQPDEHSVSVRDNAISKVDHVHTYHTKRNPADPDMSTIMDTNENNLLKTWSGWIAEPVNSGWKPETIADFAILAHKYISVQTKEPWILAALWIRDEGVAFGSQARGNSLEDRTADQRLDALLPAHAPVLWQYVEFRIKYGSEIEPKWHAEDVAMWKAAKEMARKNKPIRGAKYPENGMVIYGKYRSREQANFVPPCKTGTFGNDNKLVPGCEEVLKDVGIEFFNFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0J9</accession>
    <name>A0A9W8Y0J9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">geranylgeranyl diphosphate synthase</fullName>
            <ecNumber evidence="1">2.5.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">BTS1</name>
        <name evidence="7" type="ORF">N0V83_010382</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.29" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0J9"/>
    <dbReference type="OrthoDB" id="6921389at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:geranylgeranyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046165">
        <property type="term" value="P:alcohol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043386">
        <property type="term" value="P:mycotoxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF44">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="76"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4362289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="437" mass="48267" checksum="D59C594C367B4D4E" modified="2023-11-08" version="1">MPSTSHPFASPNAIPPRTSSSGILNLNGNSVKHRNSVLRPLSEIDWLGQSKKSKTSHSADPLNAHLQQQNYAPQSFDRPHTMASQGLNPQAMDMEPNNSQLGGGANFESSSSYPTPLSPPSDQTKGVAEHLIYGNGAAWTEEKERILLGPYDYLYGHPGKDIRSQCIAAFNLWLKVPPERLEVITRVVGMLHTASLLVDDVEDSSLLRRGIPVAHSIFGIPQTINSANYVYFKALQDLLIMNNPTLISIFTEELLNLHRGQGMDLYWRDSLTCPSEADYLEMVGNKTGGLFRLAIKLMQAESTVDIDCTPLVSTIGLLFQILDDHLNLSPTSELQSLKGHCEDLTEGKFSFPVIHAIRADPSNQILINILKQKTNDDEVKRYAMRYMESKGSFEYSRGVIEELRGRRRGLLGGLRACWRRGEGGGGGGGGGREARRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0M5</accession>
    <name>A0A9W8Y0M5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF1793-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010427</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0M5"/>
    <dbReference type="OrthoDB" id="431715at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052743">
        <property type="entry name" value="Glutaminase_GtaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032514">
        <property type="entry name" value="GtaA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033433">
        <property type="entry name" value="GtaA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31987:SF1">
        <property type="entry name" value="GLUTAMINASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31987">
        <property type="entry name" value="GLUTAMINASE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17168">
        <property type="entry name" value="DUF5127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16335">
        <property type="entry name" value="GtaA_6_Hairpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaminase A N-terminal" evidence="2">
        <location>
            <begin position="20"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaminase A central" evidence="1">
        <location>
            <begin position="258"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="617" mass="68807" checksum="216432AE091DBAB4" modified="2023-11-08" version="1">MGAPKDVELASQTSFEYTSTRSIFTIAADDKVQVKVTFLSPLTPSDLKRQSLIFSYMEVEVTSLDSSEHEVQIYTDISAEWASGDVAAKAEWGFGTTLDGIYYHKVWKQDQQVFNEFNDRAQWGNWYYATDSVDGLTYKSGSDIDVRGAFNGSGKLDNEKDTTFRAINDNWPVFGYAMDLGKVGSEAKSNLFTLGLCQDDAVQFLGADGLTNLPSLWKSYFSDDLAALSFFHKDFSDSSKLSTELDDKISQDSKAVAGDNYAIITTLAARQAFGATQLVGTEDKHFLFLKEISSNGNTQTIDVVYPATPIFFYTNPELVRLLLDPHFENQESGHYPNKYAEHDLGTHYPNATGHPDGNDEPMPVEECGNNLIMVLAYVQLSANSDYIKQHYTILKQWVEYLVEDSLLPAEQLSTDDFAGHLVNQTNLALKGIIGIEAMARMSKIIGETADADNYTSIAHDYITKWEALGINKAADPQHSILNYNNDSTHGLLYNLYNDRLLNLSLVPQEVYDIQSNFYPTVKETYGVPLDTRNKYTKTDWELFCAAIASVETRDIFISDIAKWIAETPSSHPFSDLYETDTGTQVSGIDFKARPVVGGTFALLLLDWDGYSAPPKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0N6</accession>
    <name>A0A9W8Y0N6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008571</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4364955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0N6"/>
    <dbReference type="OrthoDB" id="3782687at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4364955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="317" mass="34943" checksum="F0826D7868D9EDD2" modified="2023-11-08" version="1">MTEIQRQVARNTTSLDDLSIRGPQVSLVLLYTQELDAIRRRFASVWTEATEINLQGAMLYLFAHCLLLADHEESEAWTADTNHFVATIMQRGQSAALQLIKVIQELGFASAIDPACTKTEDGGAPLLAHPKQHFRLAFYACLFLLQYLDRNPTASVGDQDAARNAVSTVYQIFKQYVSREEVLRAADTIEVLGRSVVPGERRIKTIVKTRMGGSLNYNAVWTASKLRGRQHDPEFTVAASAPEERIESNPLVDVLGVMDNTGATSSSLDAGTPLVLGPDFVFPWGIWDDAAYDGLELGLGDHLFSDLPAPMDLFENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y101</accession>
    <name>A0A9W8Y101_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribosome biogenesis protein Alb1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010623</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y101"/>
    <dbReference type="OrthoDB" id="5304887at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053278">
        <property type="entry name" value="Pre-60S_factor_ECM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022784">
        <property type="entry name" value="Ribosome_bgen_Alb1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28280">
        <property type="entry name" value="SHUTTLING PRE-60S FACTOR ECM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28280:SF1">
        <property type="entry name" value="SHUTTLING PRE-60S FACTOR ECM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09135">
        <property type="entry name" value="Alb1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="128"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="65"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4362529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="20874" checksum="5AE93E428EDFDF80" modified="2023-11-08" version="1">MAKTAKVKKRQVTMHSRAARRAASPSIDLDKSAKPTNTTRDSASPSRPSTINPHALAAKDAGIQKKQKKGTMTRAQRLRQQKNLERAADNMDKLELKRIKSLGNEKKVKERAKGWEDVNGVSVKKNKKGDTIEADTEANRKEREWVSDEEMDGNDLTAPDGAGEVKGEGKPAEVVVPATVPLPVEQDELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1J6</accession>
    <name>A0A9W8Y1J6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_010216</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1J6"/>
    <dbReference type="OrthoDB" id="1658288at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047499">
        <property type="term" value="F:calcium-independent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185:SF1">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="5">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="4">
        <location>
            <begin position="149"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4363096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1037" mass="117626" checksum="C4BDED11299406AA" modified="2023-11-08" version="1">MLGVLDMDIDTCIRAYEDFSKDVFSDKVSAWYLRGKGRYHHKKLEDAVKRLVRTHAKSLLPDTTNLTNAELENLPMAFPTSQERQCKVFVCATDEAQSTPTFFRSYSLAGSGEAGNIPTSIVEAARATTAAPTFFEPATIGRLGARYVDGALNYNNPAKALQVEAISVWQDRKVGCLLSIGTGLQQLVDVLEGPAIIKTLRKKITDTENTHAELVKAYAGTKTYHRFDVNTGLEKLSHALREKPWLLVLDSWTPGVDETEYINLQANATVLISTTSRAVHGARLSMEMKDMDIEESTSLLLLRVSRWSSRVDDPSLRASVQDIVKDDLGCHPLAILQAGGIFENRNELDPEEFRAYFAALPNGQEDLLVTDHHEMDYRHARSVWKTFQMCFDDIKSIASRDDREGRLAATSLELIRFFALLGSGVPESFLIKAYEKLQASKSDSKQSFPLEAIRSPAPSTASELQVKVIGDACAIIDRFSLINISRSDEYRPRERIFRLHVVVRRCVLLDLRIEEQASQIYPSVWLRTVHTLSCALSFNPGIFDENLQRVAIPHLKACLEGRKLQSISHDYGEAASLDFIWLHFARIYLNHGNVRDAEVLQRDVIEYRQSKPTSEKLILEATHDLASTTRSLGKYQSARVAREKVIDRLLILRGAKGHSTNQQTPINDTLPQHIVEKMQKVDLAVGKDNFLLNAMENLAESWRDFGLHQDALKLQVLVVRTLEEHQNGSNVIDKDFDRLFAAQAKLAVIQNFTGDILSALQTRESIYATKQKQEPTQSLSLQRSKMELANSLHTAGFALRAKELRQETLDTMLNWKLYEEDPEHLDILDAKQQLASSLEVYERWEEAYDMKREVLAGKLKIFGPHHRETWKARSNVAYYYMRRGDFEKSMQEHRKVLCECEASGDTDESLKARDEIARRESSVLFRQKDELSADQLQSKVQSLHKARSKILEEWRSRHGEDFGGIGVAENLLLDVQFDFPTVFDRGEAHTLREKLLQEHEIRFGEDSEPTQFSAKFGSDETAVRKITKPLSPLKNDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y216</accession>
    <name>A0A9W8Y216_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acid phosphatase pho5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PHO5</name>
        <name evidence="10" type="ORF">N0V83_008754</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y216"/>
    <dbReference type="OrthoDB" id="433512at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17364">
        <property type="entry name" value="MFS_PhT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004738">
        <property type="entry name" value="Phos_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00887">
        <property type="entry name" value="2A0109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="369"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="404"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="505"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="49"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="545"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="549"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4365136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="567" mass="61295" checksum="8E4E88061137C6B8" modified="2023-11-08" version="1">MAANRTAGGNNAFHNYNNDFAHIEDPNERRRLALAEIDKAPFGWYHVRAILVAGTGFFTDSYDIFCVSLLTIMLGIVYKHNNKGVLPTPQDTAIKLSTSAGTVIGQVGFGALADIVGRKKMYGLELILIIIATLAQSLVGPGPGTSVVGLIIFWRVLMGIGIGGDYPLSSIITSEFATTKWRGAMMGAVFAMQGLGQLGGALVMLCLVAGFKHKLEAATSVATCTGDCQVAVDKMWRALIGIGIVPACIALYYRLTIPETPRYTFDVARDVEKANEDAKHYLSGKKGDGSPDEITQVQARRQSVAQLEVPKASFGDFFRFYGKLRNGKILFGTAMSWLFLDVAFYGLGLNSSTVLQAIGYAGGKNLYHVLYNLAAGNCILVCAGAIPGYWLAVATIDTVGRKPLQLAGFTMLTILFLIWGFAYHKLSGHAMLAIYVLIQLFFNWGPNTTTFIVPGECFPTRYRSTSHGISAGSGKIGSIIAQGAIAPLRTRGATAKNANPWLNHVMQIFSAFMFAGIFTTLLIPETKRRTLEDLAQDWDMGNESITGAEHAKHVNRSSDEGVTETKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2C2</accession>
    <name>A0A9W8Y2C2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_008564</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4364948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2C2"/>
    <dbReference type="OrthoDB" id="2544694at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000090">
        <property type="entry name" value="MFS sugar transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF78">
        <property type="entry name" value="MONOSACCHARIDE TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G02110)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="74"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="296"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="399"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="436"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="465"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="27"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4364948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="554" mass="61467" checksum="99E4A5D6FD857CE3" modified="2023-11-08" version="1">MSLLTFDQHHSWMVHSRLEKRSLLIAINCLAGMSIFFFGYDQGMMGGVNESPNYVNLMKFGYVKDDGSVVVTDALLQGGIMAVFYFGTLVGCMAGGWFGDKFGRISAIGLGAIWGIFGASLQCSSQNHNWMIGARFVNGIGTGILNGIVPAWASELSDYTSRGTFIAMEFTLNIFGVVVAYWLAYGVKFIENGYSAFRWRFPIAFQIIPLCFLLACCWAFPESPRWLCKAGRHEEALYILRRARGTEGPDAGTAEAELESIKAVIELEHKAQGTSYFSMFFGRNSGRLHTARRVQLVIWLQIIQCWTGIAGVTMYGPTIFSIAGFAPDKTQWVSGLNNIFYMFSTIICVVTIDRIGRRWTLYWGSVGQGLAMFLCGGLSRGGLDARASGNITNANQWGAAAASMVFIYTFIFGATWLTVPWLYPAEIFPLQVRAKGMAWGVVGWSIGNGTLTLVLPYIVNSINEKMFYIFGAINAISIPIVWALYPESNQRTLEEMDLLFAADEPWTWAAEKKFAELKDENSMSAISNAHHSKIRDVEAKADSPVSHIEETKRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2D2</accession>
    <name>A0A9W8Y2D2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mediator of RNA polymerase II transcription subunit 20</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Mediator complex subunit 20</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MED20</name>
        <name evidence="6" type="ORF">N0V83_009949</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the Mediator complex subunit 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2D2"/>
    <dbReference type="OrthoDB" id="1854899at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013921">
        <property type="entry name" value="Mediator_Med20"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08612">
        <property type="entry name" value="Med20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4363653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="280" mass="30400" checksum="AFBEB4F460031DF1" modified="2023-11-08" version="1">MKYSGLYYIPNPSTNLENSLATVKSLIQSIEDTFQTASRQTPWTLSYRAFRDTLPPNYTPPTDEKGSPKPYPHTYQHLLHHSSYGATRTYICIQSPPSAAGEHAKGTVTAIPAQQQDSYASLIRHQQAALWSPRHVLSVQGGAAYTTGGMFDIQVGELRASREGPQAQSGVVQSPGVIVCISTLVGSAEEASSTSNSQKPGQNGTAANGNGDDEGYAEGPDFDYAQAVIRECWKKIKEGVDLGRSEVREVMMAAREEVHGGLREQEAAVRAWCEVLKLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2I3</accession>
    <name>A0A9W8Y2I3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008532</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2I3"/>
    <dbReference type="OrthoDB" id="332863at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36142:SF5">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36142">
        <property type="entry name" value="METALLO-HYDROLASE/OXIDOREDUCTASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13483">
        <property type="entry name" value="Lactamase_B_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4365910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="359" mass="39135" checksum="3E95B230C7E84A2A" modified="2023-11-08" version="1">MSLSIKSLNGDTSFLLVFSPPVAPLSSPGLFPGSFTILLDPWLVGPSVILNPIFSISEQKTPPCISHLTELDLEPDLVLISQHKPDHCHEETLRQLPPDCESTILAIPPAAKKIRSWKHFRPELVHALPKYVEGDAQSIYRVVLPPPSPRGSCGEVTITWMPAKRDISGVHHAIGITYRPPYTVLSLQKSHYLDLPMSPPASPGSPMTIASSPRTIVPAPYNDREKTLSVIYSPHGVSYEIIRPYATSHLVSEAALPLTALLHSFDCVENPWWLGGNISTGSPGGLTIAQNLYAKAWVSAHDSAKDNRGWAVMQTRIGQYPANQVKEMLDDGHDGKSKKTANTVTVSLAPGEQYRIEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2Q4</accession>
    <name>A0A9W8Y2Q4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Deubiquitination-protection protein dph1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_007809</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2Q4"/>
    <dbReference type="OrthoDB" id="267397at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14324">
        <property type="entry name" value="UBA_Dsk2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16106">
        <property type="entry name" value="Ubl_Dsk2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000024">
        <property type="entry name" value="Ubiquitin domain-containing protein DSK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015496">
        <property type="entry name" value="Ubiquilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10677:SF3">
        <property type="entry name" value="FI07626P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10677">
        <property type="entry name" value="UBIQUILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="3">
        <location>
            <begin position="14"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="2">
        <location>
            <begin position="435"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="252"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="307"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="386"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4366174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="48991" checksum="13C3B8DABD821242" modified="2023-11-08" version="1">MADDAATSADDTQVTFNVKASNDQKHVLTLPSTTTVADLKAKLATPDFADMPAERQRLIYSGRVLKDTDTLASVKIKDGHTVHLVRGAASNARQNPANQGTASTSGASSTPTPQVPTNIAAGTGNNPLAGLTGARYAGFHGLPGMDTFGPDGGMGPPPTADSMLELMDNPMFLSQMNEAMNNPAVVDMMMQNPMIQNNPMLREMLRDPGMRRMMFSPEMMRAQLQMQRAMDGRSGGGSSFPAPGATDNTPQPSSTATGDGTTATPNQPDTTGPTDPFAAFGGAGGAAGNPFASLFAGGANPFAPHGQQTQNTTSTQNAQDASSAPPDTFPNLFGGAQGGQANPIAEAARNLMQNPEAMRSMMQMMGGGAGAGAGAGASPFSPFGQAPASTTPATGAGTNSPPANNPFAALFGPGGFGAGGGFGTPPPADNRPPEEVYETQLRQLNEMGFYEFDRNVSALRRSGGNVQGAIEYLLNGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y371</accession>
    <name>A0A9W8Y371_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008515</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y371"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF283">
        <property type="entry name" value="RETINOL DEHYDROGENASE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="324" mass="34384" checksum="53D730BB0FF0A4B2" modified="2023-11-08" version="1">MSSPPLPYNRKNALASTIASDNAPSIAGKIVLTTGVTQGGLGATFVSEIAQHAPKLLILAGRSPAKLQATAAKISALGVETRILKLDLASQAQVREAANEVLGYAEPCIDVLVNSAGTMGGPYQTTPEGLELQFGANHIGHFLFTNLIMSKLLASKAAGGPRIVNVSSDGHRFSGIRFEDPGFSGGKAYDQWEAYGQSKTANILFSKALAEKLGARGLRAYSLHPGVALGTSLAPHGFGDEDFASLVAKDKEIGWDRELDFKSLDECAATHVAAAFDPRLDRVNGVYLENGNLSDDVQETAKNPDDVEKLWKLSEELVGQPFSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y377</accession>
    <name>A0A9W8Y377_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Siderophore iron transporter mirA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_007918</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y377"/>
    <dbReference type="OrthoDB" id="2241241at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015343">
        <property type="term" value="F:siderophore-iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF87">
        <property type="entry name" value="SIDEROPHORE IRON TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="363"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="503"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4366282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="556" mass="60729" checksum="7A2E5856E52F3519" modified="2023-11-08" version="1">MTAKPQNNDDPVVSVVEERPGLATDALRTLWTPRALGCAIGCLLVMNFFMNMTVYTQNVYEPYATSHFDGHSLLSTGSIINGIVRIVAYPLLAKLADLTNHQAGGIFDSLGDTWWNITQQIFVADVTSLVNRGILFTLPESLSAIPTLYGGSYLGEHMLLSSTWRWGYGMWAIILPVTAIPTISIMLWMGRRAKRMGLIRERLSLMHGAPSGALSRTYHIVTQLDIIGAFLLIGGLAMTLLPMTIAGRNNTDRWERPSSIILLVVGVLTFIAFLVWDGKFAPNPIVPYRTIKERNVIVACVSVIFIAASDSVYRPFLSSFLQVAGHYSPGAATRVDNAQRVAFNIGALVSGICLKYVKQTKPIIMLGVGLIVLASGLPIYLTNISGTHIANEAAFITSKSILGVGRGFAQVSLQVSLQAVLESDQIAVATAVYLSSIGFGSNLGVTISGAMWNSLLLRRLNNYFSKTESQRIFGSIVVAKKYKVGSIERVAIDKCYRETQQSLAIGSVCVSSVLLVLVFFARNIKLREEDTKRELRAEKVVDSIKLEETKPNAPYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y386</accession>
    <name>A0A9W8Y386_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="16">1.3.8.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">2-methyl branched chain acyl-CoA dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">2-methylbutyryl-coenzyme A dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="ORF">N0V83_007933</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="31">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(2R)-2-methylbutanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = ethylacryloyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:65296"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:156439"/>
            <dbReference type="ChEBI" id="CHEBI:156440"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:65297"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>(2S)-2-methylbutanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-2-methylbut-2-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48256"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57337"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:88166"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:48257"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>2-methylbutanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-2-methylbut-2-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43780"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57336"/>
            <dbReference type="ChEBI" id="CHEBI:57337"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:43781"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>2-methylpropanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = 2-methylpropenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:44180"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57338"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:62500"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:44181"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>butanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-butenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:24004"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:24005"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>hexanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-hexenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43464"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:43465"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>valproyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-2-propylpent-2-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:65344"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:156457"/>
            <dbReference type="ChEBI" id="CHEBI:156458"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:65345"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 27">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="15">Amino-acid degradation; L-isoleucine degradation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; mitochondrial fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 27">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="31">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.5" evidence="16"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y386"/>
    <dbReference type="OrthoDB" id="10262177at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003853">
        <property type="term" value="F:short-chain 2-methyl fatty acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046395">
        <property type="term" value="P:carboxylic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01158">
        <property type="entry name" value="SCAD_SBCAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000012">
        <property type="entry name" value="Acyl-CoA dehydrogenase short/branched chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase short/branched chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000001">
        <property type="entry name" value="Acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF1">
        <property type="entry name" value="SHORT_BRANCHED CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8 27" id="KW-0274">FAD</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7 27" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12 27" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="30">
        <location>
            <begin position="60"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="29">
        <location>
            <begin position="175"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="28">
        <location>
            <begin position="283"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00039850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00049096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00049552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00051903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="EMBL" id="KAJ4366297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="437" mass="47599" checksum="2A766861D128440E" modified="2023-11-08" version="1">MASLSRTLRPLTRVAAASSRSLRPAVLRAAPQARCLSVTPARREAIDISEVPPTPITHLSETELLMSDSVSKFANEVILPKARDMDEAEAMDPAVVEQLFEQGLMGIEIPEEYGGSGMNFTSAIIAIEELARVDPSVSVMCDVHNTLVNTAVLKYGSERIKKEWLPKLATDTVGSFCLSEPVSGSDAFALATKATRTDNGYKISGGKMWITNSVEANFFIVFANLDPSKKYKGITAFIIEKGTPGFSIAKKEKKLGIKASSTCVITFDDVEIPKENLLGKEFEGYKYAIGLLNEGRIGIAAQMTGLALGAWENAAGYVWNDRKQFGQLIGEFQGMQHQMAQAWTEIQAARALVYNAARKKEAGEDFVMDAAMAKLYASQVAGKVSSSAVEWMGGMGFVREGLAEKYFRDSKIGAIYEGTSNIQLTTIAKHLQKQYTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3J1</accession>
    <name>A0A9W8Y3J1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptidyl-prolyl isomerase cwc27</fullName>
            <ecNumber evidence="7">5.2.1.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CWC27</name>
        <name evidence="7" type="ORF">N0V83_007531</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family. CWC27 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3J1"/>
    <dbReference type="OrthoDB" id="442970at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF6">
        <property type="entry name" value="SPLICEOSOME-ASSOCIATED PROTEIN CWC27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="6">
        <location>
            <begin position="18"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="185"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="232"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="471"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="383"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="471"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4367001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="494" mass="54404" checksum="0A3B749D30DC1C9B" modified="2023-11-08" version="1">MSATYNLEPNPTAKVVLHTTAGDLELELFAKQTPVTSRNFLQLCLDGYYDNTVFHRLVKGFILQGGDPTGTGQGGNSSYNGELFADEFHTRLKYTRRGLLGMANTGKKNDNGSQFFFTLAATPELQEKNTMFGRIAGDTIYNLMKMAEADIREGSEDEPMYPTRITGADIIINPFEDMVKRVSVAERTEPEARKPKKPKRKAGKNVLSFGDDEAADSAPVAKKAKYNTKLVSAGDETPKSRAPEQAKAIPFRKSTRTSPSPSPPPKTAQKTAPRSPPKEKARPPPPRELSPAFEKQSKLDHVNAQIAELKASMKRSTTAQDTQPTKKKSLLESMVPTSTTRGRKRGAGGNAAQERATLDILSKFKSKLESAEPPPAAKAKSPTFSEDTSNRNGSGNAPSAPEEDEDACDLHFIVGCQSCKAWDKQDEEESDDDAGWMAHSLSFAKDRLGKDLEWKRKNEEELLVIDPREEAKRHKAEGKGMKEWDKGKVNKMKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4F7</accession>
    <name>A0A9W8Y4F7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_007177</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4F7"/>
    <dbReference type="OrthoDB" id="406156at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000040">
        <property type="entry name" value="Chromosome 21, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00682">
        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Non-haem dioxygenase N-terminal" evidence="3">
        <location>
            <begin position="48"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Isopenicillin N synthase-like Fe(2+) 2OG dioxygenase" evidence="2">
        <location>
            <begin position="230"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4367592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="383" mass="44042" checksum="5158387CA8077D0D" modified="2023-11-08" version="1">MATTTIEADTAKLSVADDFPKYSFDKLEPYVHPPETKEDLPWSELVTLDLEDYGRPGGKERLAKQLEHAVHHVGFFYVKNFGLTQEQVDRQFTLAKHFFELPTSEKEKFEVDYKAADYNGWRRPGRSVVIKTKDNVEIYNIPKFTDDFKGKYNQPDLLKAHLPEIEIFQRALHSNVVLPLLRLFAIVLQLPDEEYLVKQHTYDKKSEDHFRYMIYHPRTEEEWAEAKYGATGGHTDLGTVTLLFRQPVAGLQILGEDGEWTWVSAQPGTITVNLADTISHLTGGWLKSSVHRVVAPPKDQRQYERTGLLYFARPHNDTKLLPIVDSPVLEKAGVKPRFEKIVTVEEWVKAKQTLQLNPDIAAKRWADGKVEVIAGFHDQKYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4Z4</accession>
    <name>A0A9W8Y4Z4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">S1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_006401</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EIF1AD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4Z4"/>
    <dbReference type="OrthoDB" id="1738325at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039294">
        <property type="entry name" value="EIF1AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21641:SF0">
        <property type="entry name" value="RNA-BINDING PROTEIN EIF1AD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21641">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1-like" evidence="5">
        <location>
            <begin position="24"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4368046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="136" mass="15364" checksum="647293F21BB58398" modified="2023-11-08" version="1">MPRPKRNVLATIEDTITPPDTLPSGHLIARITKAEGKNLYNAELPDGKPILAELEAKFRSTVWIKRGSYVVVDTSALAERDNKLDGEIVNVVRDEKAWRKMSYWPKEFAKKSTYVEDSDEEESTVGKMPPTDSDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y535</accession>
    <name>A0A9W8Y535_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="9">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_006952</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y535"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000037">
        <property type="entry name" value="alpha-1,2-Mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF29">
        <property type="entry name" value="ALPHA-1,2-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="331"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="448"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="502"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="588"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="405"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4367371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="598" mass="67830" checksum="2CC93BB7CB9C3F33" modified="2023-11-08" version="1">MPRVRTRHIAICAVILLWVYYFLPRDEAFTYVYDYGHGASHGSTVAVARYMNDPEFIPLIIATQSSFDWSSVNFTYPPKLTPKLPATFTKRPRIQHKFKRDSRNVAAVQEQRRLEVKRVFEKSWTSYRSRAWMKDALKPISGEYVDQFSGWAATLVDSLDTLWMMGMRDEFYEAVAAVSTIDFGESTTATVNTFESCIRYLGGLIAAYDLSGHEVLKTKAIEVGDLLYAGFNTKDGMPVDFIHFEDAKRGVGLIVESQVVSASPGTITLEFSRLSQITGDDKYYAAVSRLMDVFYQGQNDTQLPGMWPMYVSMSKQDVVSGYQFSIGGNADSLYEYLPKAYALLGSVDASSKKYEIMSRTFMDTAERHLFFRPMIPGNEDILISGNVDILEDGPTLDPESEHLSCFIGGLYALGGRLFNSKKYLDTGAKLARGCAYAYRSFPTGIMPERYNMVLCPGPDPRKPCAWDEEHYTTAAAERVQYKSYLPKGFTTAKDPRYILRPEAIESVFILWRITGDAVWRETAWEMFQAVVNATDTRYANAAIMDVNVIGSKQEDYMESFWMAETLKYFYLCFADPDLISLDDFVLNTEAHPFRLSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y555</accession>
    <name>A0A9W8Y555_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Serine/threonine kinase</fullName>
            <ecNumber evidence="8">2.7.11.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PKC1_3</name>
        <name evidence="8" type="ORF">N0V83_007246</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y555"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20822">
        <property type="entry name" value="C1_ScPKC1-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08689">
        <property type="entry name" value="C2_fungal_Pkc1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000014">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037778">
        <property type="entry name" value="C2_fungal_PKC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046987">
        <property type="entry name" value="Myo9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46184:SF5">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IXA-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46184">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IXB-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="C2" evidence="6">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="7">
        <location>
            <begin position="221"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="118"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4367661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="333" mass="37004" checksum="AADB39093FB64218" modified="2023-11-08" version="1">MTRKPLSGHLSLRIHAVADVDHAATGRFSRGPETFVNIKVEDAIKGRTKPTRNDRWTDEVHDFSIEKANEIEITVYDKTGDHLLPIGMLWVRISDIAEEMRRKKIETELQNSGWVSADKMGGHSGIQPDLQFQPPPGQSFSGAGGPAPGGMRPQGGGGGGGPQPQTGPVMIDDWFSLEPAGRIHLTMSFAKFTGNKQPFDLGLGRKGAVRQRKEDVVEQYGHKFTQQQFYNVMRCALCGEFLKYAAGMQCSDCNYTCHKKCYPKVVTKCITQSNAETDPDEAKLNHRIPHRFENFSNMGANWCCHCGYILPFGRKQTKKCTGKASFALNAELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5I1</accession>
    <name>A0A9W8Y5I1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_007165</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5I1"/>
    <dbReference type="OrthoDB" id="250329at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45667">
        <property type="entry name" value="S-ADENOSYLMETHIONINE MITOCHONDRIAL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="4"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="103"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="204"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4367581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="37877" checksum="F292BC4DE48704C9" modified="2023-11-08" version="1">MSENTNSDILIAGAIAAFTVDLLVYPLDTIKTRIQSPNYARIYTNPQTGKPNPALFRGVYQGIGSVIIATLPSSGAFFTTYERTKALFTRLNATSSSPNGLLPIAAVHAGASSLAELVSCAILTPAEVIKQNAQMVSHGSPTNATLQTLSKFKSNPLALWRGYAALAGRNLPFTAMQFPMFEKLKVTIREYRDRRGLTTGTIYESGMITAVSAGSAGAVAAIITTPIDVIKTRIMLAAVDDAPASHKPAAASQAKGKADGLVDAFGKSVESTKASAKSMGSALNPLAKSEQRGRKSSWKIGQEIVAERGFKGLWRGGALRGIWTFIGSGLYLGAYESGRVFLATRRGEHVNEEEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6B8</accession>
    <name>A0A9W8Y6B8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glycine--tRNA ligase</fullName>
            <ecNumber evidence="4">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Diadenosine tetraphosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">GRS1</name>
        <name evidence="16" type="ORF">N0V83_007399</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2 ATP + H(+) = P(1),P(4)-bis(5'-adenosyl) tetraphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:34935"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58141"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6B8"/>
    <dbReference type="OrthoDB" id="57698at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070150">
        <property type="term" value="P:mitochondrial glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00774">
        <property type="entry name" value="GlyRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00858">
        <property type="entry name" value="GlyRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.200:FF:000001">
        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000004">
        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.230:FF:000002">
        <property type="entry name" value="Glycyl-tRNA synthetase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000010">
        <property type="entry name" value="Glycyl-tRNA synthetase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033731">
        <property type="entry name" value="GlyRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003211">
        <property type="entry name" value="PRK04173.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745:SF0">
        <property type="entry name" value="GLYCINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="GLYCYL-TRNA SYNTHETASE/DNA POLYMERASE SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="15">
        <location>
            <begin position="162"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4366871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="657" mass="74089" checksum="258D0216BC7F711C" modified="2023-11-08" version="1">MAGDMTTFKGKPFDRASLESLMKRRLFYTPAFDIYGGVSGLYDYGPPGCALTNNIVDTWRKHFVLKENMLEVDCTMLTPHEVLKTSGHVDKFADWMCKDPKTGEIFRADHLVEEVLEARLKGDKEARGQKVDVEEEDPKKKKKKVKNIAAVKLDDALVQEIEEVLAKIDNYNGEELGLLMKKYDIKNPATNGDLLPPVAFNLMFQTSIGPSSNMPGYLRPETAQGQFVNFQKLLEFNQQQMPFASASIGKSFRNEISPRAGLLRVREFLMAEIEHFVDPEGGKKHPRFEEVKDVELELLNREVQLSGKTTVETTKIGKAVETGLVDNETLGYFLARIQQFLLKIGVDSKKIRFRQHMANEMAHYAADCWDAELLTSYGWIECVGCADRSAYDLTVHMKKTGAPLVVREPRKEPLKIEEWQCDLEKKKFGPKFKKDGKTVEAAIEALSQDLREKLSLDLEKEGKIVIDVPGVGDGKVEVPKELIKIEKRTRVENVREYTPNVIEPSFGIGRIVYALCEHIYWTREQDEARGVLSFPPTVAPTKVLIVPLSSHTDFKPFVRTLSDKLQDIGISSRVDDSGASIGKRYSRNDELGTPLGITIDFQSVKDNTFTLRDRDSTQQVRASLDEITKAISSLVNGTKKWSDIAASLPRFEGQEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7E2</accession>
    <name>A0A9W8Y7E2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006162</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7E2"/>
    <dbReference type="OrthoDB" id="4676at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016813">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03884">
        <property type="entry name" value="M20_bAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010158">
        <property type="entry name" value="Amidase_Cbmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01879">
        <property type="entry name" value="hydantase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494:SF5">
        <property type="entry name" value="ALLANTOATE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494">
        <property type="entry name" value="ALLANTOATE DEIMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="509" mass="55797" checksum="EF4E7FFB6C7D954B" modified="2023-11-08" version="1">MASPASRFTSAFRIANRRYPALRCATLSRARIGQDHIRSFASSPRWQIRTKEMNDDLLKDLKVNQGRLMEDIHYTCQWGTGERWGDAPTETGMSRLALSDADKAARDWFVQTTEALGCKVTIDAMGNIFAVRPGLRNDKPPTFVGSHLDTQPTGGRYDGILGVTAGVEMLKVFADNWVETEYPVGVVNWTNEEGARFPKSMVSSGVWAGDIPVEDAHNLREVHPGIATMASELKRIGYLGTTPASHEATPMAAHFELHIEQGPLLEMANKKIGVVTGVQAYKWFTVNVTGRDTHTGTTDLKSRADALLTASKMILHSHRLATANSALASTGILNLKPGSTNTVPGLVSFSLDIRAPKDTTVAKMKDLVLRDFPLIASGEDVDGSNHGCTPSLPLTVDIVEDFNSPTTTFHSDCITSVNQAAHHILGPNSHNLVMDMTSGAGHDSVYASKRCPTSMIFVPCREGVSHNPREFCKEVDCALGAQVLLESVVRFDRMRDERRVSWKQEPWTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7I2</accession>
    <name>A0A9W8Y7I2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_006764</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7I2"/>
    <dbReference type="OrthoDB" id="288203at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="326"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="6">
        <location>
            <begin position="386"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4368407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="561" mass="61057" checksum="5C8B5D173F1157C4" modified="2023-11-08" version="1">MSRPTSLMGLLTAEIIRDVTDDGYTPQAIASAVAMSVGIYALVIGMLKLGFLLEFISAPVLSGFISAAAIVIMLGQIPSLFGVTVRTGTANIIHDLFAKIPQYDGPTCGVGLGGIVMLVLMQKMGQRWGGKNKAIWLLALGRSAVVLILFTGISYAVNKNIDDDPIFALSKVKSNGINSPVMPDGKLISKVFPSSIAPFIAASIEHLAIAKAFGRKNGYVIDPAQELVYLGFTNFFNSFFSSMAVGGAMSRTAVNSDSGVKSPVYGLIAGGVVILSIFKLSPALYWIPKATLAAIIVTAVWHILVPLRVFYGYWRTSLVDFITSMLAFWLTLFVSSEVGIGTAVGFNIVYHLIFQAFHRVRRVTSIQIHTPSRPAGVQGIPLDTQVFRIHQSILFYNAYRIKSQCLDNVQTYNSGDIETLEAQLANRNWSVAGDRRAISLRTKADILEEPVQIRTVVLDMGRVHNIDTTGLNALKDLKVDIEKFGGKTTQLRLVGVQDRVRERFARYGWKLLDVGEAKKDKKATVVYGSVEEAVFMSAGRAAADDITPVEVRIVGSEKEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8I8</accession>
    <name>A0A9W8Y8I8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006707</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8I8"/>
    <dbReference type="OrthoDB" id="5422579at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4368351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="613" mass="69854" checksum="AA233B93CE84A708" modified="2023-11-08" version="1">MAAKAGVTKRRVSTVLAASGSSVPSMSFMEGLFGDEFPEAFATSGGRAARNSNSVTDLPAELLAMICEHLSNLDIKRLRLAGRYLAANVDLRIGRAYISPNSANLNCLQKILGHPRYRNRVLEVVWDDAQLEEYPTLDSFRHAISIDEAKTKQDIEKRLEGAISGYEDANSEYRALERDDFFSRDGQLTEVAKGILLRYGDQLSRDMIARNKTIMSVEQSYDLYQRLYREEQEIIERGLDVVALRHALTGFPNLRRITLTSEVWRPWNLYPHYDTPFYRSLPPGFRKPSVWPWLGERPGATPAQGAHREKVLSERDIGKLPKEFRGYNILVSTLLELLPSIRLEEFIIDAGKERIGIPHRLFTPKSSSLEETVRMFRLVPLKRLQLVFNPDGFVRRPDCDNALNLIKQTLSELHHLEHFDLSLQWNPRRLSPGSTGWYINATIILPPATVRNLKIFALRNAFVLEDGLVALVQHLTSAEHITLDNVMLMRIHGAHAQNQEDLTSTEFRLFNRLKEHYAASGSGVSHPRFEWRQPIDEPKEYRRCNLVGEEIGAFLYDGRECPFEDHTGLEFIKRGVGWVVDDRDRKFCVRKGEEGMGDVGTYYATDELGTNGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8L9</accession>
    <name>A0A9W8Y8L9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ribosome biogenesis protein tsr1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TSR1</name>
        <name evidence="7" type="ORF">N0V83_006331</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class translation factor GTPase superfamily. Bms1-like GTPase family. TSR1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8L9"/>
    <dbReference type="OrthoDB" id="119302at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012948">
        <property type="entry name" value="AARP2CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030387">
        <property type="entry name" value="G_Bms1/Tsr1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF1">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN TSR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08142">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22298">
        <property type="entry name" value="Tsr1_G-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00785">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51714">
        <property type="entry name" value="G_BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Bms1-type G" evidence="6">
        <location>
            <begin position="39"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="380"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4369246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="617" mass="69488" checksum="F635D5E0B9D49F96" modified="2023-11-08" version="1">MAPQVMSKFDRRNRAKQMRLNKDNEHSRSTNVFAGKDGAPRVVAIIPLCSDVSAAAAVRSLNSSLDIEEEVPEAGWTRTNVDRFKQKVQYLVVQRDLLATLDACRVADFVVFVLSAQEEVDAEGELILKSVESQGISNTFTVVQSLEKVEPAKQRPSVVSSLKSYITHWLPSTERVYSLDNRQEASNLVRSLCTTTTKGIRWRDQRSYMFIEDIAWPGGKTAVSGDGTGEVVLTGVVRGLGLKADRLIQVGDWGDFQVEKIVAAPLEVKKRGKGEVMEIDAEADNTLERPSEDQDDLADLAPEEAIMDDVTNYAPSMAPTERKGVLLDDHHYFSDEEEEYEKVRPKRLPKGTSTYQSAWYLDDLSDSGSDLEDYDMEDVAEAGQSQPAHPADGVEGMDLDGKAMTEGGPSEYPQSEMFLDPSPEDEAEQIEAYRKSKRTEADEDLEFPDEIELHPNVNARERLIRYRGLKSLKTSPWETEEDRPYEPEEWQRLLEISDYKRTATKFMREAWAGGVKPGTRVSVHLRGVPLQFQNAPRPLAMFSLLRHEHKRTACNYSILLSSEHEAPIKSKTELIVQCGPRRMVINPVSLRRPFSKHIQLLTTTALLPSRQHAQQRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8U0</accession>
    <name>A0A9W8Y8U0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Laccase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_004366</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8U0"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13850">
        <property type="entry name" value="CuRO_1_Abr2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13876">
        <property type="entry name" value="CuRO_2_Abr2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13898">
        <property type="entry name" value="CuRO_3_Abr2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000036">
        <property type="entry name" value="L-ascorbate oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF488">
        <property type="entry name" value="LACCASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040839379" description="Laccase" evidence="7">
        <location>
            <begin position="18"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="10">
        <location>
            <begin position="27"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="8">
        <location>
            <begin position="166"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="9">
        <location>
            <begin position="471"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4371150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="605" mass="66445" checksum="BE6BEF254983DC6F" modified="2023-11-08" version="1">MISLIVLWLSLVAGTLAGSCNFKLELTWGKGSPDGYERDMIFINGQYPGPLLEIQEDDWVEIEVINHLPFNTSIHYHGIHQTNTPWADGVPGLTQRPIQPGASFKYKWYADQYGSYFYHAHSRGQIDDGSYGPMYIKPKAGAENPFHLIDPAEVDLLKETEASVAPMILSDWRHKTSDATWQDQLASGIESAICMDSLLVNGKGAVDCWSREDINTFTNPAIVPLLQANGLQMTNKGCLPPKMFQILLGNSSTINPDALSPSVFEVCTPTQGSREVVKAPQDKKWLALDIISTAGIDTFAFSIDEHQLWVYAVDGHYIEPLKVDVLTVANGDRYSVFIELNKKSGNYGIRVASLSLAQLIDTTAVFSYGGDYGNGYPPYKNGTQGSVVTSTPYTTRAGAPTAGDVIVFDQAQMVSFPPQYPTTAPEIDQTFFMHTGTIGNSYTWALNSTPFNHPMIDDSNPPLLYQLPNANNPGGDITIVTKNNTWVDLIFLVPGGNTPPHPIHKHSNRGFIIGSGEGQFNWTSVAEAAQAIPQNFNFVSPPYRDGFVTPPSEVDPMWLAVRYEVVNPGAFMLHCHIQSHLNGGMAAVILDGVDEWPEVPDDCKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8Z4</accession>
    <name>A0A9W8Y8Z4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Short-chain dehydrogenase/reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_004457</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8Z4"/>
    <dbReference type="OrthoDB" id="542013at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF252">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE FAMILY PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G08550)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4371240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="344" mass="37726" checksum="E82762D2AF7E8B65" modified="2023-11-08" version="1">MAPSKDMQPLSSPFFPSIFIKNQFRTKTAWPPKDTNLTGKVAIVTGANQGLGLEACDQLLSLRLSSLIIAVRSKAKGEAAADKLRAKYPKASIDVFQLDMSSYDSIQAFVAQVEQLKRLDIVILNAGIRKMKRELVPSTGHEETIQVNYLSTSLLSILLLPILKRKKSAEGSPGRLTIISSGLAFAAKFRNRAAVPLLPSFDEAANFDVMDQYNNSKLLGMMFLYKLADYVSADDVVVNIVDPGFVRGTSLSRDMSAVVGAIMSVWKNIAARPVDVGASTYVDAAVVKGKESHGSYIMSWEINPFAPLLYTPEGKQIIERLWEETMTELSFADVQGVLDSLKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y991</accession>
    <name>A0A9W8Y991_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Beta-lactamase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_005635</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta-lactamase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y991"/>
    <dbReference type="OrthoDB" id="5946976at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058664">
        <property type="entry name" value="ARB_00930-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051478">
        <property type="entry name" value="Beta-lactamase-like_AB/R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22935:SF95">
        <property type="entry name" value="BETA-LACTAMASE-LIKE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22935">
        <property type="entry name" value="PENICILLIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26335">
        <property type="entry name" value="ARB_00930_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040797526" description="Beta-lactamase-related domain-containing protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-lactamase-related" evidence="3">
        <location>
            <begin position="59"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-lactamase-like ARB-00930-like C-terminal" evidence="4">
        <location>
            <begin position="416"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF26335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4369871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="574" mass="63887" checksum="0416DA72D86E7C72" modified="2023-11-08" version="1">MVLLSMLLFAALANAKCFAPSVAHPLPEYDRHDPLLQEAFESIDTALTVAVAAPEFARTSFSVAITSSKESLWSHHHTARERNTSRSDIARVNGDALYRIASITKTFTVLGILYQHEAGNMSLDDTVNNYIEELGEKSDGGIPWKDITLRSLASQLSGIPREFAQGDLINSDKFLLNDPTDIGLPPVSRQGLVTCSEYSPNYEVPCTLEDLLNAVKSKAPLFAPNQQSTYSNVAFELLGLAISRVTNQSFEAYINNAIFKPLNMSSSTLSVPPDSAGVIPFEPQYWDVDEGIQAPTGGIYSSTNDLSKYLRYILQHFNAISHAINWNHPVSPSRGLNSFYGMPWEIFQTDRILKDSKRTVRFVTKSGGVPGYTSIIMTVPEYDLGITILLAGTPGFFQQIREIVTVAIVRAAEKLAIRQLNERYAGTYISTNVTLNSSMTLVADHRGLAVTKWISNSTDVLESISRIAMLKNWYAQLVPTLLYRDEKEQKGEEWRLQSTEESSEGVGEIWDDFCITNIDAPLYAGVGFNEFVFWDQGKDGAFKQLELTAFRANMTRLDDGGDRLRQEEQEVLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9E2</accession>
    <name>A0A9W8Y9E2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9E2"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="241" mass="27390" checksum="D0A70F88605B1F43" modified="2023-11-08" version="1">MVDTLLDTAEDETDALLEEATDDEDATDDEEAADEEAADEEEATDDEATDDEEATDEDEATDDDETTEDDEATEDELATLLEGVLEATDELDGVDETVEEDVNLAGVEDEAEADRVEDEADRVEDEADRVEDEAERVEDEADRVEDEAERVEDEAERVAEREAERVEDDTEREAAREDDERVEVLKVEEPRVLDERTVDERTVELDLEPPLHFPYPELHPVPQYFVLEPHHPYLERSISHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9J6</accession>
    <name>A0A9W8Y9J6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004474</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9J6"/>
    <dbReference type="OrthoDB" id="3752733at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="140" mass="16076" checksum="75058D2EB6C8FD88" modified="2023-11-08" version="1">MEPTTATPTKRASSDPTDPRSVTKRQSRLASLFMSLLAVSPMAVEYDAADQKTIEAAQEAVTQPTPPKKPYIPLVIRVSPSEIRKTRWKRFKYEMGLKMKSRHNRKGKERNKWMQIEGVEMGELNGEERERQNAKRDDMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9M1</accession>
    <name>A0A9W8Y9M1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NodB homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004519</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9M1"/>
    <dbReference type="OrthoDB" id="9970124at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43123:SF3">
        <property type="entry name" value="NODB HOMOLOGY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43123">
        <property type="entry name" value="POLYSACCHARIDE DEACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NodB homology" evidence="1">
        <location>
            <begin position="74"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="328" mass="37536" checksum="0C5BFD4E3A6BF4C7" modified="2023-11-08" version="1">MVHVEESLQLPRDFEGFGEHGHDAQWPNGARIAVSFVLNYEEGGERNVLDGDGTSEPYLWEKGPTGGGREHRHLNGEQDFEYGSRCGAWRIHRLMKEFGWNMTLWAIAVAMERNPTFAKACVRDGHEIGAHGYRWLDIWDYSLEDDKAYIKKTCKALEAATGEFPVGAYFGRGTPNTASLLPIMWKEMGHKMLYTSEVYNEDSPYWIDLPWEKGLPEEKKEGLLMLPYNYDCNDGKFHMLPGFVSSAGNTYEQYLKDTFDCLYREGGKMMTIPLHSRIAGKPGRSESLRKFMKYISEKEGVWVANRRDIANHYRKTFPYKAGSKTGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA48</accession>
    <name>A0A9W8YA48_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003793</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA48"/>
    <dbReference type="OrthoDB" id="5428055at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="449"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="480"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="545" mass="62482" checksum="2F593CF206117D66" modified="2023-11-08" version="1">MASNGNSDISGATKIEEASTATPSRNVTHSPYQKYIEALSGSNPGLKKADPNNKNGPLKNGNALVVLLDAPATGQPGFNTTEFKSATDLRHHFQNANHEHDHRRIYIMEGLAPDYVSIIGGHFFMEPTFFQRQERTCVWSNDFTPVSDALPQPSLLDPDKSFHLQYCELRQFNKAIENRYYFCQRTRRHVGMTAPRHKEESTMGILRRKVSWWCRETGNGGWDVVILCDPQLLHLSPDPKEVVRGENNDIKNVRDELKNGPFQEGYVDFLPPVSYDKIGSKKVHPHTSMLRDLIHYYQQHSDMLSTDDWDTPNMSAIFLKKIVAAHYLQLVDYIKAMLPSLELRLTTAWVEEQDQWKSLQTISRRCGNYRDDIEDTLLSLGYPLDGKLDVASKLRSTNWKDCEKDFHYVYFRLKILKERADNLMQAMTGLASIAGNRQNLEEAKRVKRLNLLALLFIPLAYTSSLFSMQDAYAAGQNKFWVYWVSAIGAVAFTAAVTWILDSALDDEAQWTWRPLTYLKFWGGKDKKPKAMKRKATGFYDHNVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAP5</accession>
    <name>A0A9W8YAP5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_004458</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAP5"/>
    <dbReference type="OrthoDB" id="2108at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050596">
        <property type="entry name" value="AspAT/PAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383:SF1">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="6">
        <location>
            <begin position="80"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4371241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="484" mass="54664" checksum="EDCE96D9D8B7D14F" modified="2023-11-08" version="1">MSFSAEKVNHLKGAFSQRRFSSSKDPSESELGSAIHRQFRQSHEGHKPHAGLDASRASTGVVWTSEQAYKHGFLEKPEEWANLGQGAPEVDDNIEGCFERPKEVDISAHGREYGPTAGIRPLREAVAKLYNEHHRKWRKSQYTWENVCIVPGGRAGLIRIAAVLGNCYLGFFIPDYTAYNEMLSLFRNIAAIPVPLGEADQYHMSPDKIAEEIARGTSVILTSNPRNPTGQMVVNPEGTKLAQIQNICRDRATLIMDEFYCGYNYTTNCDGTVISAADNVEDVDEDDVLIIDGLTKRFRLPGWRVAWVLGPKEFIQAIGSCGSYLDGGCNVPFQEAAVEMLSPPRVRNEMRALQMHFRTKRDYVIGRLQEIGFKFPYMPNSTFYLWLDLSSLPEGINDGLNFFQACLEEKVIVVPGIFFDLNPSRRRDLFDSPCHHFVRLSYGPRMDVLEKGLDSIEKIVKKYRENPNKHRQMQVRPRGTGAPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBA4</accession>
    <name>A0A9W8YBA4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GST C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003361</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBA4"/>
    <dbReference type="OrthoDB" id="5595695at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03190">
        <property type="entry name" value="GST_C_Omega_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.520">
        <property type="entry name" value="Amidohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.90">
        <property type="entry name" value="Amidohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10910">
        <property type="entry name" value="Amidohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047047">
        <property type="entry name" value="GST_Omega-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016639">
        <property type="entry name" value="GST_Omega/GSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419:SF25">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419">
        <property type="entry name" value="GLUTATHIONYL-HYDROQUINONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01206">
        <property type="entry name" value="Xi.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01148">
        <property type="entry name" value="Xi_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="1">
        <location>
            <begin position="621"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="776" mass="87882" checksum="55A8486721A1B341" modified="2023-11-08" version="1">MASFLLRDVRVFTGEQTIEEGYVYVEDGKIKSVGPVSKAPQTASKIYSKPGHTLLPGLIDCHIHADMADPKALPQALRFGVTTVCEMQNELVNVRKLKKQALEPDTASYKTAGQAATIENGWPIPVILAHNDSPEVLAEIAKWPKLSNRENVIEYLEWNTKEMQPDYIKLMHESGTMMGRKLDYPTVELQRTIVSEARARGYLTVAHATSLQDTLDVLEAGVDGLTHSFCDKPPTQEVVDAYKKNNAWLNPTLAAMGSLTTEGKDLQHQFAQDPRVKGLIDEDRVGNMCQCMAFALEHGKVEHAYENVKVLRKAGIDILCGSDSAGPAKGTAFGLSMHHELHLFVHKVGMSPVEALRSATSLVAKRFKFEDRGRLEEGLNADLLLVEGNPLQDIDTTLNIRGVWRDGKLCSAHASKLESGFKKKFFDDARRNISKVRMAESHKREQGEVEGSRLVNDDTHKYSEKDGQFRRKASAFRNFISSDPNSDFPAEKDRYALYIHKGCPWAHRTNIVRSLKGLQDAIQLIEWDISMIHEGKGWGMSGRPGFETEPLYGFTNSRQLYEKADPKYEGRYLVPTLWDKKKETIVNNESSEIIRMFYTDFDSFIPEHLRESTKGDKGIFPSHLRKDIEAMNEWVYDTLNNGVYKCGFATSQEAYEQHVYPVFKSLDRLEEHLGQPGHGPYLFGDFITEADIRLYPTVLRFDVAYFTIFKCNLKMIRYEYPRLHQWLRTLYWDESEVTNGGAFKHTVDFSKYREGSASAIRSKIVPVGPKPDILPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBE3</accession>
    <name>A0A9W8YBE3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SH3b domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBE3"/>
    <dbReference type="OrthoDB" id="3797359at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003646">
        <property type="entry name" value="SH3-like_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08239">
        <property type="entry name" value="SH3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SH3b" evidence="1">
        <location>
            <begin position="309"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="468" mass="53266" checksum="0FF293497C0906DC" modified="2023-11-08" version="1">MSSPERTAVQFESALDQLEELRPDEYIIAFGPNGEQFCGTPNGYSATSLPGIVLDLFYGASRVVWASFGSNRDSWFFTCEAKNGNRAFYCGDGIPAALLQFLRQLNVSQAVNSSLRVQLGGSESFVVWVGTTWACHNVPGLLRVKLCEMSSASHEWNGVTRGSLMSGTLNNVQWHHSGVYYIKSGNRHIWDFQTDIFRAGWYLLWNEPASGKLELEVKNDLAYTAIDPHAPTGETFVFIKKQEGRKEAPFLMHFEHERRLHTNLGSKDCAPKPIMSVQHMPKKSDIHYQWAVSKKSGRPHPRESRELFLDKGDRLKVLKDMGRDWYIVSSKKGTKGWVHGSWLDFGDRKLHADPKSAYNQFREDLQKLLVPGQLCKFPAMASYIDACTRVECQLLKEDVGSVGICLHDLMVLLEGSGRYSYELLKEERNVWHPDRFVRFCHADHVDRLKPMAEEMFVLYGILMDRCKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBM9</accession>
    <name>A0A9W8YBM9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YDG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_003425</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBM9"/>
    <dbReference type="OrthoDB" id="3244603at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.280.10">
        <property type="entry name" value="SRA-YDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036987">
        <property type="entry name" value="SRA-YDG_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003105">
        <property type="entry name" value="SRA_YDG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51015">
        <property type="entry name" value="YDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YDG" evidence="4">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="175"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="252"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="367"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="444"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="532"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="661"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="183"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="264"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="549"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="671"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4373134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="701" mass="76914" checksum="7E51FA262E2E31B0" modified="2023-11-08" version="1">MFAYHAGIIGLESVEGGITFDQEGAYAIVLKERREVEGILEDRFTYRCPLNDKGKFKLTSATQRSREPIRVLRSHSVNGIWGPKAGIRYEGLFSVKGWSIRTAKTTSTTGEWKVGDIFFEIRIERNDDTPVEELMVHPTATEVDDYSEYKRLRRVYREQKRNGPEPTSSIKQDLQLAAKAAPPIQPAQPLMPPSAAPEASRKPSPSTLHSSMFRQPKFDEGSHVPPSPGQEVVSPMAVTNVNQFSLTMRKRLAVAPAQSNRRPSPSPTGPPPSDLEHVTPPVSYDSSNNTGVSADNRINIREVAPWIEYDVGLNIPSPASQAPAITTTTREAVVTPHSLASTSTSAEEEMRSAIKAKETIAKPATVVTPNPLNSQQRRKSDDNKGMTVATLSPTGTASRRDGTKSILLRSRNPISKLFDGVKENVEEDYFGDAVVDMVRTASVGEIETPKTPPKLPPRRHQRSSSDNIMRVLSPTQTTPTKDLFFTSDGGDESPLSPFAMRRRRAIYPPSPPAHHNDYNNNHFSPFFPTTIPPPPPPRTHTQIHDDGKSTTATTMQQPKSRLEEASNDPFVVSPLLPLTSHVPMSPLPVPLQNVIAPPPVLLQSRTASLTPFLAERKHGRQRNHGSENEKETSNKWPTLQMMEGAVGLEVLVEFRDPWCSAGGDGDGDSGGSVSPRAAAVAASRRRMERQSSVSVAPHVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCK5</accession>
    <name>A0A9W8YCK5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peroxisomal d3,d2-enoyl-CoA isomerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_002966</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Mycotoxin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCK5"/>
    <dbReference type="OrthoDB" id="448450at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000048">
        <property type="entry name" value="3,2-trans-enoyl-CoA isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051053">
        <property type="entry name" value="ECH/Chromodomain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43684">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43684:SF3">
        <property type="entry name" value="PEROXISOMAL D3,D2-ENOYL-COA ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0843">Virulence</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4374225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="31197" checksum="1566E42CE19B0D61" modified="2023-11-08" version="1">MTSTADYTDIKFEIKHQKIGIITFNRPKALNSFGGNLINDTIAALRVLDSHPDTVFTVLTGEGRFFSAGADVKGITGLTGGDGEAANAKNPAEKKLAFLGRFGYATELLRSMIDHRKVLILALNGPAVGGGAAWFPGIADIILASHTAWLQCPFSALGLVPENGSALSFAQSIGIHRANDFLMFGRKLSPQELLDAGMYNYVWEEEGAEFQEKVVGFLQGQLEVNDGKSMMEMKRLQNAPVRDARLVAVVNAVDALAERFVEDAPKKRFEEKRRELEAKRAGRAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCR1</accession>
    <name>A0A9W8YCR1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DUF221-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_002733</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCR1"/>
    <dbReference type="OrthoDB" id="2150324at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF149">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G11660)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="391"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="440"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="484"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="599"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="657"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="688"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="10">
        <location>
            <begin position="38"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="11">
        <location>
            <begin position="213"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="9">
        <location>
            <begin position="395"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="879"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="961"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="968"/>
            <end position="977"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4373994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1001" mass="113490" checksum="21685595A0E6AB79" modified="2023-11-08" version="1">MGFDMDLRIRQTTGESSTDKFLDLIADPFQAEVQQNSIYAAIVYSFAVSGLLVIVFCFLRPRNNRVYAPRAKHADEKHRPITLGKKPFSWLAAVKDVKEQDLVDTIGLDAVVFLRFLRMIRNIFLVLTAIGCGILIPINVVGGSPFYKQWSSIPTLMKFTPQYIFGTKFWAFVIVAYLFQGTVCFFLWWNYRAVLKLRRAYFNTSEYKASLHSRTLLLTHIPSSSRTDAGLVELVEQVKSTDDMPRTAIGRNVKDLPELIKAHDEAVRELEQHLAKYLRNPNRLPATRPTCKVAKEDIATHGKGKVDAIDYLTDRIARLETTIKEVRESVDMRNPMSYGFASYAHIEDAHSVAHASRKKGPGGCDVYLAPKPHDLLWQNLPMSRRTRRMRAFWDGLWIVLFTIAFIVPNILTSVFLSDFSHLGLVWPAFNTNLQAHPTGWAIAQGILAPLVQTLMYMGVPVVFRRLFTHAGDVSRTSRERHVTARLYAFFVFNNLLVFSVFGSAWRFVAGVIAAQDQGIWQAIQDGHLFTKVMTGLCNVSTFWLTWQMQRNLGAAIDLSQAWIFTWSWIQRKFFSPTPRELIELSAPQPFPYADYYNNYLFVSTVGMCMGTLQPIILPITAFYIAMDCVFKKYLLQYVFITKTESGGRFWRLLVNRMLFAVLFANAVIALVVGAQGVGSINSVENGNMLYAMVPLPFLLFGFKWYCKRAFDDKLVYYSTLPYSDLEGSHHVDHPKERRSNKVSVRYGNPALYKRLLTPMVHAKAQHLIKEIYGHRSNADGDIFEAPHRRSTDRARPATPGYQHYGDMFMTEMNHDQPGKQQQTTSGEAMPAIEVVAESELDFENFKKRAEFREQFGGEGQLYGRPDDLVSTRPGTPSTFTTLTDLHNGGGPYRHRDSTGSRASSRTRLGDKEDAEQQGTLYAQGYQRTPRAADDGFQSVGVDVSIPATPLDADDVLRATGSRQPLVGGDDDGDELDDGGLGYFDRRMRDEDTSYERFRFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCS8</accession>
    <name>A0A9W8YCS8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002788</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCS8"/>
    <dbReference type="OrthoDB" id="5309037at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42354">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42354:SF1">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="146" mass="16782" checksum="8119033BC93CD0DA" modified="2023-11-08" version="1">MKERAEAAVETAKSVLQDLVVVVSLVRTLTDSFGSASDLYRKLKRKSHAKDSDDEKVQERQVRRPLRPRRDSKASHELGHRYQTGWSDSRKDDYSDNDEELICTSSSQVRAEYDRGYRKLGEPFARGDFIEKAKVLRVYFAHGSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD85</accession>
    <name>A0A9W8YD85_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1996 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002695</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD85"/>
    <dbReference type="OrthoDB" id="74764at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018535">
        <property type="entry name" value="DUF1996"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43662">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43662:SF4">
        <property type="entry name" value="DUF1996 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09362">
        <property type="entry name" value="DUF1996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040864636" description="DUF1996 domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1996" evidence="2">
        <location>
            <begin position="36"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="348" mass="37160" checksum="1DF0B438D6661EEF" modified="2023-11-08" version="1">MQWATLLAAAAALAQNAAAQNMLRFACSQLVVERTDPLVNPGMKYTPHLHQIVGGDSFNVTMDPNNDPAQLSKCTSCTFNQDKSNYWTAVMFFKAKNGSYIRVPQIGNGGPQGKLVNDGGLDVYYIPSGKVTAFKKGFRMLAGSASNTDASKVKSGAICHRCWTSASENTFVGGAPCSGSDTVAIPKSPTCKLIRQTIIFPTCWDGKNLDSPDHQSHVAYGQGAGANGGGACPSTHPTKLPQVMYELMWDVSKFADQSMWPTDGSDPFIYSMNLGGSAAHGDYVFGWEGDSLQKAMDNNCNLNTACPKAGLTVQTSAQYGACKKKQQAPEDVDGWLKAMPLGEMAVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDE3</accession>
    <name>A0A9W8YDE3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="9">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">srs2</name>
        <name evidence="15" type="ORF">N0V83_003065</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDE3"/>
    <dbReference type="OrthoDB" id="1470711at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17932">
        <property type="entry name" value="DEXQc_UvrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18807">
        <property type="entry name" value="SF1_C_UvrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.160:FF:000001">
        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.486.10">
        <property type="entry name" value="PCRA, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070:SF2">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE SRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 11 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="13">
        <location>
            <begin position="6"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="14">
        <location>
            <begin position="280"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="723"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="820"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="881"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="929"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="891"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="980"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4374324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1021" mass="113252" checksum="2456E3D74B1F8CC5" modified="2023-11-08" version="1">MDVLLQGLNDAQKDAVTSPVSVVQVLAPPGSGKTKTLTARVAYHINHDRLQPWNIIVCTFTIKAAREMKDRIRGFVGEKMESKLILGTFHSVARRFLSRYGHEIGIDKNFGIADTEDSKAIIKRIVTRYQYTVEPGHARSRISRLKAKGTSAESFAAKSKKVDDNEFAQVYSSYQEHLKASNLLDYDDLLVRCVELLQRHPSCVSTIQAVLIDEYQDTNNIQYELMKLLAQRTRRITIVGDPDQSIYSFRSAEIQNLYRMREEFPEAVVINLENNYRSSGYILGAALAVIEQDESRPEKALIATHCVGEQPALRHLATVNVEAQWIVDEIKRSKTLSAGLLNYNDYAILVRSANLSLSIERELGRAGIPYRMVGGKRFFDRAEIRVILNYLRCISNPDNNDAIVGVINTPSRKVGDKTVKGLLEEAEVKKVSLWRLVCDIAQKRKTPTTKLSTPAEQGITLFFRLINNARKKLLPVAGEPCNLLDLISYILTQTSFEAYLKQSYKENWKDRWANVEELVGQATQMATALAEGEEIGDDALPAVDGIEQRPDTTVDTLSKFLVNVALATEVERDDGEELSQVTISTIHAAKGLEWPVVFVPAVYDGSLPHSRAENHDEERRLLYVGMTRAQALLYLSCPVKQSGQEQTTLSKFVSSKSVQKYFSQRGPDFAFKRSTVPDLATILRRPCPALEDIEAARKLLERLEDDKYPATREEIDGVDPSWGASWDDGFSGSGPGGNGTQPSFKRRKLETSSLGYTEKVSITMHKSSGFSTASTTMQSVKAGFTSALDLGNLQAMQQEAERIRMLASAQSAEAGRQVYNEAKKTESKPARAKALRSRPTGQGAITSFFKQSNSSLSDEADYNPSFSVLSGLASLHDISNVRPSVPRPTQPYHSLSIPTTHRLQTRPILAKPKRSDPDPSAANTRYVLLSSSPAKSEHEDPTSVLNTGEDMADPESPVKKATSSEVGGTIGRGGFRPASTFHNTSISQVQSQEAQRKTLGTRRTMQGWSVKHSQMPKPRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEH0</accession>
    <name>A0A9W8YEH0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">N0V83_002864</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEH0"/>
    <dbReference type="OrthoDB" id="6770063at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20335">
        <property type="entry name" value="BRcat_RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000082">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF134">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="255"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="384"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="460"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="485"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="16">
        <location>
            <begin position="130"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="652"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="656"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="540"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="559"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="572"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="583"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="618"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4374123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="834" mass="94026" checksum="AD503911E6BE94CB" modified="2023-11-08" version="1">MAPDPNINIADSSGVGLDGSNNGTGIAIFPGVRSGSVEELGTYDPVTDIDTELEQEDFSNKKERHFGDYGPQDGGQDAEIVWEYLTFETELPHPTTIHPTQTGQELPPEPPNLVKFTNPFDWSEKRKNFTVWISCVITALTAFSAGSYSPGVGQMTEEWGVSNVAALVGITTFTTGFAVAPMVLAPFSEINGRRPVFLASGVLFVICQLCTGLTRSYAGMLVVRFLTGVGGSTFSTMVGGVVADIYHAEHRNTPMALFSGSALFGTGWGPLVSGFIAQNTTWRWIFYMQTIMCAMMVALICVIFNETRGSVLLSKKAKTLNKWYEARESLGYYGVNVSNPEKPGTTVSQRIRWKVRADEERSSLAKLIGISLYRPFHLLFTEPVVFWFSLWVAFSWAILYLTLAAIPLVFQHNHGFSLEQANAVFAAMCIGSMIATVLSIYQEKIARKYGKLVSTPEGRLYFACVESACMPIGLFMFGWTSFQDIHWIAPAIAVTIATIGIFAIYLSTFNYLADTYHRYASSALAAQSFCRNILEEKNEPSFRASSPSQYYAHRSGRARVHDEARTLDTKARSRPSKQTKHKTQAESKASDRPKSEERSSQKDLPKAPDLDHLRSRQAENPQPTNKLKIQNRTGKHSNSSDHRKHRRPQRKETKECIICTDTRSLHRFPNRPPTTQCTHEVDTCRRCLRKWIQSEFTTKIWNEINCPICSKRLQHSDMREFAPRDVFRRYDKLSTRAALEAIPNFRWCIAKGCKSGQVQPPGVVRFRCIACKKSHCVEHNVAWHKRETCKEYDYRTNKRYKKEEEVLSKKLILETTKKCPGCKRSIEKSYGFEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGA1</accession>
    <name>A0A9W8YGA1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000014</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGA1"/>
    <dbReference type="OrthoDB" id="5194044at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018811">
        <property type="entry name" value="MRX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28002">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28002:SF1">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="246" mass="27542" checksum="DE472C1331ABC594" modified="2023-11-08" version="1">MEAYPKPRHQYIYTPRYGQRGFLSYAGQQPNGAYPAGVIGGYGYGAGVGYAGAGVGVGVGVAVGGGYYPGAYAARPNYPYSYGGNYYGGVGGYYNYGLYGSTYPLANFYYPYQRTYYNSTPSYGYQQAYVYPPGPSTTTTTTTYHVTNSQAPTYSHAAPRVETRAAYNHGPTREDAQMENRRIATERGAYDARRIKPADARSDDPFWCRERNGEWHLRTYYQIENECHPGRWMMDANQGFLVFNRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGT8</accession>
    <name>A0A9W8YGT8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein AF-9 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 6" type="primary">YAF9</name>
        <name evidence="6" type="ORF">N0V83_001254</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for an H2A variant leading to transcriptional regulation of selected genes by chromatin remodeling. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histones H4 and H2A. The NuA4 complex is also involved in DNA repair. Yaf9 may also be required for viability in conditions in which the structural integrity of the spindle is compromised.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the SWR1 chromatin-remodeling complex and of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">The coiled-coil domain is required for assembly into the NuA4 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the YAF9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGT8"/>
    <dbReference type="OrthoDB" id="16041at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1970">
        <property type="entry name" value="YEATS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038704">
        <property type="entry name" value="YEAST_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005033">
        <property type="entry name" value="YEATS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055129">
        <property type="entry name" value="YEATS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23195">
        <property type="entry name" value="YEATS DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03366">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51037">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1 2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="YEATS" evidence="5">
        <location>
            <begin position="9"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="174"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="234"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="234"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4375974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="277" mass="31519" checksum="3D0BF59A7E2AD80E" modified="2023-11-08" version="1">MVAPAANKRVKNKRVSRNIIIGSEAWQLPPVGHPDRPKNVPPDHTKRWTVYVRQPEGDPALTTWLNKVQFKIFNTYENPLRTCENPPFEVTETGWGGFSIDIRLHFIPASGEKAQYRQHFLQLEKYGDEAMQAEQERTGCVRSEFLEVVQFNEPTEALFDALTAEDQWNYLMPAGKGGSKKASLGANGRMKRGMPNGERSAQLPEKGAEEVPFSQDQEQALIDLLKSKIEQVDKELEKETKKREETENKLKDLRNELGHEAAQQAAHQASGDRSRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGY6</accession>
    <name>A0A9W8YGY6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AP-2 complex subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_000619</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <location evidence="1">Coated pit</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGY6"/>
    <dbReference type="OrthoDB" id="28053at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000020">
        <property type="entry name" value="AP-2 complex subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050840">
        <property type="entry name" value="Adaptor_Complx_Large_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017104">
        <property type="entry name" value="AP2_complex_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003164">
        <property type="entry name" value="Clathrin_a-adaptin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009028">
        <property type="entry name" value="Coatomer/calthrin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22780">
        <property type="entry name" value="ADAPTIN, ALPHA/GAMMA/EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02296">
        <property type="entry name" value="Alpha_adaptin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037091">
        <property type="entry name" value="AP2_complex_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55711">
        <property type="entry name" value="Subdomain of clathrin and coatomer appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0168">Coated pit</keyword>
    <keyword evidence="3 7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin adaptor alpha/beta/gamma-adaptin appendage Ig-like subdomain" evidence="9">
        <location>
            <begin position="728"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="30"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="75"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037091-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4377789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="969" mass="108284" checksum="E62BFB6095A417E0" modified="2023-11-08" version="1">MATSAAAKMASSAGGGLFSRNQSGNVPTMRGLVSFIADLRNARARELEEKRINKELANIRQKFRDGNLNGYQKKKYVCKLLYIYILGWNVDFGHLEAVNLISATKYSEKQIGYLAVTLFLHEQHELLHLVVNSIRKDLLDHNELNNCLALHAIANVGGKELGEALSAEVHRLLISPASKAFVKKKAALTLLRLYRKYPGIVQNEWAERIISLMDDPDMGVALSVTSLVTALVQDNTEQYKGSYVKAANRLKRIVVDNECADGYFYYKVPCPWILVKLLKLLQYYPPPAEDSHIRKLIREALQKIMDSALEMPKNVQQNNAQNAVLFEAINLVIHLDTEQDLMVQISQRLGKFIASRETNVRYLGLEAMTHLAARSETLDPIKKHQAIIIGSLRDRDISVRRQGLDLLYSMCDPTNAQAIVNELLRYLQSADYAIREEMVLKIAILTEKYATDVQWYVDISLRLIAMAGDHVSDEVWQRVIQIVTNNEELQVYAAQTILQYIKADCHETLVKIGGYLLGEFGHLIADTKGCSPIEQFIALSAKMRGCSSSTRAILLSSYVKFVNLFPEIKPQLLQAFRAYSHSLDSELQQRACEYLTLATMPTDDLLRTVCDEMPPYPERASALLSRLHQKHATTSDKRTWVVGGKDANADIKEFGLARQNTSAGIKRSFTEPVSKDHKASTTNGTNGVAAISSDLEGLDLDIDTNKVLRAPNLASAAHLSPDWEIGYNRLLLRGEGVLYEDQQIQVGLRTEYRGQLGALIFYFTNKSSFGIGSFTTTVDNRSSETLKSDIKGLPDTSIAPEGQTQQTIMFECKNVFTDPPTIRISYLAGALQGLTLQLPVLLHKYMEGAELSAEDFFKRWKQIGGAPREAQSIFGLIGKNRTMNSEFVHKVVKGFKWGLVTGVDPNVKNIVGATVLHSSEGKFGCLLRLEPNFDTQMYRITIRATDESVPPVLIKAMEDRLAQGIEGMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGZ3</accession>
    <name>A0A9W8YGZ3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_000361</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGZ3"/>
    <dbReference type="OrthoDB" id="48317at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08249">
        <property type="entry name" value="enoyl_reductase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047122">
        <property type="entry name" value="Trans-enoyl_RdTase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348">
        <property type="entry name" value="HYPOTHETICAL OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348:SF2">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C2E1P3.01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="4">
        <location>
            <begin position="15"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4377536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="351" mass="36753" checksum="78130E5831AF7217" modified="2023-11-08" version="1">MAAPTNRAAWLTKAGTPLEVSDAPMPTAGPGQIVVKNAAIAINPLDWHMRDRGDFVQQWPAIVGCDVAGKVHETGPDVGRFKKGDRVIGHAINLVTGRPQDGAFALYTVLQADKAAILPDKVSFTDGVVLPIAIETAVCVLSLKVPGEVMPGVATPALGMPYPSLEEPVPSVNKTLVVYGGSSSVGSMTTQLATAAGINVISIAGAHNFDLSKRSGATEVINYKDATFVDKVVEAVRRTGHEFVGIFDAISIPETYANDLAILAQLGGGHLVCSHPPPSDVPVNVKPGMIFAVNDIATPVWKDYVTSALQTGKLQCLPPPTIVGNGLEYIQEALDKLKAGVSATKLVVTLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH49</accession>
    <name>A0A9W8YH49_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CBM1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_000100</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH49"/>
    <dbReference type="OrthoDB" id="413885at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030248">
        <property type="term" value="F:cellulose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09630">
        <property type="entry name" value="CDH_like_cytochrome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1210">
        <property type="entry name" value="Cellobiose dehydrogenase, cytochrome domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.410.10">
        <property type="entry name" value="Cholesterol Oxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000254">
        <property type="entry name" value="CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035971">
        <property type="entry name" value="CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015920">
        <property type="entry name" value="Cellobiose_DH-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053208">
        <property type="entry name" value="GMC_Oxidoreductase_CD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47190:SF2">
        <property type="entry name" value="CELLOBIOSE DEHYDROGENASE (AFU_ORTHOLOGUE AFUA_2G17620)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47190">
        <property type="entry name" value="DEHYDROGENASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00734">
        <property type="entry name" value="CBM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16010">
        <property type="entry name" value="CDH-cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00236">
        <property type="entry name" value="fCBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49344">
        <property type="entry name" value="CBD9-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57180">
        <property type="entry name" value="Cellulose-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00562">
        <property type="entry name" value="CBM1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51164">
        <property type="entry name" value="CBM1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040976413" description="CBM1 domain-containing protein" evidence="4">
        <location>
            <begin position="24"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="domain" description="CBM1" evidence="5">
        <location>
            <begin position="860"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="212"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="791"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="791"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="847"/>
            <end position="858"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4377276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="898" mass="94464" checksum="0AED9840EC6F546A" modified="2023-11-08" version="1">MKGASVLKAASLLSPLLASVTLAQDKFTHPGTGIEFWRQVVGDDQTAGGFEWGYALPGAATATNDEYIGYIKGSLETGRQGWSGVSHGGGMPNSLLLVTWPLNDTVQTKFVYAGGYVAPDDYTGNATLSQISHSINDTHFELVYRCQWCWVWNQKGAEGSQLPTSDVQVIGWAQHNKIFSGTWSFHTKGQSQFGASVADARNAKYDDWVKLAPGTPSGSPTATPSGGVTPTPTPTGPVTCAGKPAPTGSYDYIVVGGGAGGIPVADKLSESGKSVLLLEKGPPSLARFGGTMGPEWVTSNNLTRFDVPGLCNQIWVDSAGVACTDIDQMAGCVLGGGAAVNAALWWHPVDIDFDYNFPTGWKSADVKPAIERVFKRIPGTDTPSMDGKRYKQEGFDVLSGALGAGGWKSVVANNQPNEKNRTYSHSPFMYENGQRQGPLGSYLVTALARKNFALWTNTMVRRVVRTGGKATGVELESGVDGTGYCGTINLNPGGRVILSAGTFGSSKVLFRSGIGPKDQLTIVKNSAQDGSSFINESSWINLPVGQNLNDHVNTDLVIKHPNISFYDFYKAWDTPIEDDKQAYLTKRAGILAQSAPNIGPLAWEIIKGSDGIDRSLQWTARVEGPLANDTHSLTISQYLGHGTTSRGALSINGALSIYVSKAPYLQNQADTDVVIAGIKSMLAAIQKNPAIEMEVPPANTTVEQYVASLPKTPAARRANHWIGTAKIGTDSGLTGGTAVVDLNTQVYGTDNIHVVDASIFPGHIFTNPTSYIVTIAEHAAAKILALAGSGSGGTPSASAAPSSSAAGKPSSAQSSAASAKPTSTKATKSAKPKPTHTKTSAKPVTPAKTTVQPASPVQTKTADAWQQCGGSGFTGATACVQGYTCTKYNDYYSQCVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHF3</accession>
    <name>A0A9W8YHF3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF2433 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_000812</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHF3"/>
    <dbReference type="OrthoDB" id="3918848at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018829">
        <property type="entry name" value="DUF2433"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052743">
        <property type="entry name" value="Glutaminase_GtaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056812">
        <property type="entry name" value="RRM_fung"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31987:SF11">
        <property type="entry name" value="DUF2433 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31987">
        <property type="entry name" value="GLUTAMINASE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10360">
        <property type="entry name" value="DUF2433"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25061">
        <property type="entry name" value="RRM_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2433" evidence="2">
        <location>
            <begin position="297"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="RNA-binding" evidence="3">
        <location>
            <begin position="562"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="621"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="433"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="485"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="519"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="621"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="675"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="702"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="717"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4377982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="727" mass="76555" checksum="0D3D9FBFAC78EAA7" modified="2023-11-08" version="1">MPPHMTSMLTAPKQAPNQPRPYPGNYLPNGGAPGAPSGPAPGAVPLLPNQGRVIQQGSVRVLCIADVRGNLQSLNQLAADARANYIIHTGDFGFYDDRSLDRIAEKTLKHVAQYSPLLSDNVKRSIASAPPQPPIKERFAREHLPLSELPLFLNKTYSLNVPVYTVWGACEDVQVLEKLRSGEYKVDNLHIIDEAHARLLDVGGVKLRLLGLGGAVVMHKLFDNGEGRTTIAGGQGTMWTTLLQMGELVDTANRVYDPTETRIFVTHASPAREGLLNQLSVTLKADFSVSAGLHFRYGSSYNEFSVNPTLDHYRGKLAASKASFNDVWDTVKTEVEPAVADSDSQQRLLALALDIVNKMPTVANGGNPFGGPAPGPQSGVIDESAFKNMWNFNLADAAYGWLVLDIDNGRIGTEMRAQGFNFAHRGGKVAPGQTQPAAANTSTAPPASVPSPAAQPSPAHGGPRGPPSNAPQQPRPAVATPPVPQQQQQIKPAQPVRTGPSPAPAATPKEPVKAATPQPSTSTNNAPANAAAEKAIPSEVKPNGTAAPEKGPASPAPREPVERKESKGLFIAGVKDEDEAKGLLAEEDRSKIEKMEKIKHFFVAHFATPADMQGALRRVADDVRNARGGPEKQTVKIYNERPNNAPRYGAGSWQASNRGGVQSGGYRSGGASDSEGGRGGRGGRGDRGGLRGGRGGERGGRGGRGGRGSFRGGAGEGAAAASTGGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHG4</accession>
    <name>A0A9W8YHG4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHG4"/>
    <dbReference type="OrthoDB" id="5375886at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4378175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="83" mass="8943" checksum="7C4F07C1D866E081" modified="2023-11-08" version="1">MKPTQIPTKLLKMSAPQPGRQSPDPERQSDAQQGQTSDNVNKQGGAPSEGNAQQVSDDHKEGLSSNPTHPLEKHAEETTSKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHK4</accession>
    <name>A0A9W8YHK4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">6-phosphogluconolactonase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000681</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cycloisomerase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHK4"/>
    <dbReference type="OrthoDB" id="9972196at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050282">
        <property type="entry name" value="Cycloisomerase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019405">
        <property type="entry name" value="Lactonase_7-beta_prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011045">
        <property type="entry name" value="N2O_reductase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30344:SF1">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30344">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10282">
        <property type="entry name" value="Lactonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50974">
        <property type="entry name" value="Nitrous oxide reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040865089" description="6-phosphogluconolactonase" evidence="2">
        <location>
            <begin position="19"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="393" mass="41263" checksum="EA29FD7B27D3A123" modified="2023-11-08" version="1">MLPTHSLLLPLFGSVASAANLWATHYSGTINYLTFSGKDLKLSSSTRTGNNLPSWITYDGPGKALYIPDEVFYGPSTGNLVSFAIGNNGTLTPTGKAPTPQGVVATALYGGADGKGFIANAHYQSSQLTTFKLPLTGAAPQQTLKYTMNGKGAVPSRQDAPHPHHAFVDPTGDFLLVPDLGADLIRINKIDKTTGQLTECSPAKTAPGTGPRHGAFWTPATASSRVRRAAEGTTLFVANELANTISGWSVSYPSGGCLTLTVKQSLTPYQGNSSAATGTKVGEVHVKGNFVYTANRNDKKFAPNDSLSQYTISSDGTIAWAGISSTYGTYPRTFDINKAGDFVAIGDQTTANVAIVSRDPVTGKLGDHVADLRISTVGTPENEDGVSAVVWDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHY1</accession>
    <name>A0A9W8YHY1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Serine/threonine-protein phosphatase 2A activator</fullName>
            <ecNumber evidence="10">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Phosphotyrosyl phosphatase activator</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">RRD1</name>
        <name evidence="12" type="ORF">N0V83_000774</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for PP2A-like phosphatases modulating their activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2Ai) in presence of ATP and Mg(2+) by dissociating the inactive form from the complex.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the PTPA-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="10"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHY1"/>
    <dbReference type="OrthoDB" id="16120at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008160">
        <property type="term" value="F:protein tyrosine phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04087">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1150:FF:000003">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004327">
        <property type="entry name" value="Phstyr_phstse_ac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043170">
        <property type="entry name" value="PTPA_C_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037218">
        <property type="entry name" value="PTPA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03095">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016325">
        <property type="entry name" value="Phstyr_phstse_ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140984">
        <property type="entry name" value="PTPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0697">Rotamase</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="368"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="374"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="391"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="408"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="422"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4377944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="442" mass="47876" checksum="40EB3597410808B9" modified="2023-11-08" version="1">MASHTATAPALQLLDAHDGYNFAVPAKRINDGDDVSFFLASKAYADIMTFIFQLNTAMLPRKVKGENEGTGSVKEWTLNDSQVPCPQITQSLAKLLEALTAIIDEAPPDPGPRRFGNVSFRVWYDLVRERISGLLDQYLPDNVLAFKSTSEVPVKAELEAYLIGSFGSSQRLDYGTGHELSFLAFLGCLWKLGAFPESIDGDQERGVVLGVIEPYLALVRRLILTYTLEPAGSHGVWGLDDHAFLPYIFGSAQFSPAITNPGDVAAEGSLSDAPNPADVAKSNAVERERRRNMYFSAIGFIYDVKKGPFWEHSPILYDISGVKAGWAKINKGMIKMYNAEVLSKYPVVQHFPFGSLFAWEKDPKAKAIQASVHTSSQPKSSTPSSTAPTARPQPPATKPQPPLRDPLAGGGTTGWGEHGYTSSLGDGASSSRCIGACGAEWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIB3</accession>
    <name>A0A9W8YIB3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000691</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIB3"/>
    <dbReference type="OrthoDB" id="3782026at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="189"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="287" mass="31728" checksum="97F89006A2B684E7" modified="2023-11-08" version="1">MQDTSAAVAHNGKAFLISIAAPHFEARKGKNPDSFLSPKLPPTIPSSKQFISLFHTVKMSLTFLGYFRFLITYEFYSKVGFLISYTIASLLVLEVLRLVYTDANKREVEKMLDAYTQSRSSSGYTQKGQTSNKISIEDLEKQHPQTPPRPQRRPSSPAATPESEKTLVNNEARRRPQRAHSSASQLQHPATAPAPTSAPQTQTPTPTSPPTRRLRKRSDTVTSTSSSSESKKLERRNRREASPASSSAAGSSKASSRHASPTAVRRVRDKEAQVLSSSLHKLLRQRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YID2</accession>
    <name>A0A9W8YID2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA polymerase II transcription factor B subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TFB2</name>
        <name evidence="11" type="ORF">N0V83_000893</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the TFB2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YID2"/>
    <dbReference type="OrthoDB" id="364513at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040662">
        <property type="entry name" value="Tfb2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004598">
        <property type="entry name" value="TFIIH_p52/Tfb2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13152:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13152">
        <property type="entry name" value="TFIIH, POLYPEPTIDE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03849">
        <property type="entry name" value="Tfb2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18307">
        <property type="entry name" value="Tfb2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor Tfb2 C-terminal" evidence="10">
        <location>
            <begin position="385"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4378063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="486" mass="54236" checksum="41D3D7A37EF05833" modified="2023-11-08" version="1">MSALWKLAPWFKPSARKEKEQALFILDRLHILTSKRNDDGRSMYSLSPGFQRSLRQAIEGSGTHRSFGVPATREESGGKRVTTNFLDEYSRAQWEGILYYLVSGAAGLQADGISRSEVGPGTKKLLAAGDLVRTMHGSPRITKDGFSFVLQETNAQVWSLLIVYLKMVNEVSATNPLCLYQRHLKDPYPDKPHSNDKQLGMSETEVLAFLFMLGSLELGQDYSTSTLSPTQLQMLEDLSAMGLVYRSSKDAKTFYPTRLATTLTSDSGSAMSASTNDIAQANQATSAVNPSSSSTTLQNKGFIIIETNYRLYAYTNSLIQIAILSLFTKLQHRFPNLVSGKLTKESVHKAVQSGITSAQIISYLTTYAHPQMQKNVPYIPPTVMDQIRLWEYEGERVEVTMGYLMREFGSEAEYRDVLGYADALGVLVWQKDESRCFFVSHVEQISAYLKKKKENREGGRGGKRIGLMGIVHIWRFLGTCTCMGIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YK33</accession>
    <name>A0A9W8YK33_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ubiquitin-specific protease doa4</fullName>
            <ecNumber evidence="5">3.4.19.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">DOA4</name>
        <name evidence="5" type="ORF">N0V83_000785</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YK33"/>
    <dbReference type="OrthoDB" id="292964at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00158">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000125">
        <property type="entry name" value="Ubiquitin C-terminal hydrolase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF23">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE USP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="403"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="4">
        <location>
            <begin position="701"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="151"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="257"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="259"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="346"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4377955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1082" mass="120626" checksum="D88844BB55E4A02E" modified="2023-11-08" version="1">MSAAAPLPPPGPSPAAPMAGYTNGADGWRGGDGAADPRGPAGPFPPIAEIAGSATEMAESLRHHSIRLLLEKGKTHFHSAKAMVGNRSSPAGAYWEYLVAYQIVSDLIPRHPDYYEKLENSRGQTYRDFKELTRDIRSSEERFMRIKDIIKNDNKRNGAQHKSTSSTSSILSLRPGSRNGTTIPRRDDELMLPDVPTMLPSGRGSPAVPFDPSARKPPVQAKPQSLHGRAVHQSVPSLNGASDLAQRFANLRGFAPPVDTSSARSSQDLSVKMPSPADYHSSSRPSGPRVMPPPPLPKLPLNTQFAASLPKEPSPTYSPARNISNPASINPPRSTPRSMVGTGGRSNSLAASSASSHAPNTNGTPDSYFPTQPGVPSTAGRMRSVSKSIELEIDASKLYDYFRMYNVLVIDVRDRAEFDAGHIFGRNIICIEPLTLQDGDSAEQLLDRLVISPDEEQVMFDRRNEFDVVVYYDELTPNTGFMHKHQRTESELALKRLFDTLHEFNAEKPLKRPPVLLKGGIVAWIDLVGPQALKMSTTAALVAGTKTRSRALRRSPAATHVARSNLHKRRRREYVPMDPEEEQKWLEEARKGRTAVDIPAADGDEEDEPVSPFYRSTEEFLRRYPDVEAEQSMVYPSSKPQAMNHYVAPAIPAAPSRPAPSVPRVSYSGVHERQLTGQGRNNQPPMYLSQPRFSNVRMHKTGLLNFGVTCYMNSVVQCLSGHLLLSDLFLSQRYQRELQRDNWKGTKGILPEAYATLLSNLFKGDISSVRPSTFRRICGHFNSQWGIDEQQDAKEFLEFVLDYMHEDLNVTWNKPPLKPLTDQQELQREQFPRPYAAKIEWGRYQHRDLSVIGGMFAGQHASQLTCQTCGITSTTYEAFWSISVEIPREGQCDVRDCLRSYCAAERLAGDEVWRCPRCKKDREAVKKITITRAPDTLVIHFKRFSASRTESARKVRTAVHFPLQGLDMGPFVEPPMTPEQEVYVAQQAHDGPTQLAGVKSDPTMNGPFVYNAYAVIWHIGATLGSGHYTAFVKDKSRGCWRHFNDDKVVDFEPGNLPSNQRLQNEKAYIVFYERERVAGGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CG63</accession>
    <name>A0A9W9CG63_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_010708</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CG63"/>
    <dbReference type="OrthoDB" id="5126878at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4361768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="360" mass="39965" checksum="6A6B54B4FD7C3FD9" modified="2023-11-08" version="1">MIDFFNGATRSMADPGLDLLRKAILSLAVTFFGSQHRQERITNQGYAQYGEVLGQLNRHLTTPERQLTNETLLTAQTSSEMLYVHPKLPPESYKPLIDDIDVLLENLESLYPTWKELNRSQLTKVNGLSKPAKELGITNHVSATAYMLYNTVYICIMQIKQSLSPSPDYVALRNAAAMKIARCLELKEYEMREGAPQSNTITFVATKVAWQALGGFNTVEGRRLAQVVRSAVNGVFTRPYSLDRNQMRTDLPTPDTTFAQYVAIIPPDSSANSLNAAQMMQVWEHQLDVINMGHKQTTTPPHSILSLETSSNFLLNMGQGPPTPPHSVSSLETSPDLLPDAEEARYALATHLAARSMAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CGB0</accession>
    <name>A0A9W9CGB0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Arabinogalactan endo-beta-1,4-galactanase</fullName>
            <ecNumber evidence="3 6">3.2.1.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010859</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>The enzyme specifically hydrolyzes (1-&gt;4)-beta-D-galactosidic linkages in type I arabinogalactans.</text>
            <dbReference type="EC" id="3.2.1.89"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyl hydrolase 53 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.89" evidence="3 6"/>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CGB0"/>
    <dbReference type="OrthoDB" id="110914at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031218">
        <property type="term" value="F:arabinogalactan endo-1,4-beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015926">
        <property type="term" value="F:glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000077">
        <property type="entry name" value="Arabinogalactan endo-beta-1,4-galactanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011683">
        <property type="entry name" value="Glyco_hydro_53"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34983">
        <property type="entry name" value="ARABINOGALACTAN ENDO-BETA-1,4-GALACTANASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34983:SF1">
        <property type="entry name" value="ARABINOGALACTAN ENDO-BETA-1,4-GALACTANASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07745">
        <property type="entry name" value="Glyco_hydro_53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040989040" description="Arabinogalactan endo-beta-1,4-galactanase" evidence="7">
        <location>
            <begin position="16"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4361918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="355" mass="38433" checksum="C53FBB6667763EB3" modified="2023-11-08" version="1">MRLTSLVLFAASALAALPYKGVDWSSLLIEEKAGKTYKNSNGQTQPLETILKASGVNMVRQRIWVNPTNGDYNLDYNLRLAKRASAAGLPIYLDFHYSDNWADPGKQVTPSAWTSLSRDALVTQVYTYTKSVLDAFAKAGIPLSIVSIGNEITAGLLFPIGQLSNGADGPKNVAALLKSASKAVKESTLATKPKIMIHLDNGWNWDTQQWWYDTVLGSGGGLSTSDFDIQGLSYYPFYNSAATLAALSDSMGKMATKYGKEVQVVETNWPSYCPNPAYAFPADTKSIPFSVDGQVTWMKEVAKRVAAVAGGKGTGLFYWEPTWIDNPGLGSSCGWNLMVGSDGKVMNSLAVYQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CGP3</accession>
    <name>A0A9W9CGP3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Inositolphosphotransferase Aur1/Ipt1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010597</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CGP3"/>
    <dbReference type="OrthoDB" id="2566866at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd03386">
        <property type="entry name" value="PAP2_Aur1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026841">
        <property type="entry name" value="Aur1/Ipt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052185">
        <property type="entry name" value="IPC_Synthase-Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310:SF7">
        <property type="entry name" value="PA-PHOSPHATASE RELATED-FAMILY PROTEIN DDB_G0268928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14378">
        <property type="entry name" value="PAP2_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="374"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Inositolphosphotransferase Aur1/Ipt1" evidence="7">
        <location>
            <begin position="150"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Inositolphosphotransferase Aur1/Ipt1" evidence="7">
        <location>
            <begin position="228"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Inositolphosphotransferase Aur1/Ipt1" evidence="7">
        <location>
            <begin position="348"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4362503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="444" mass="50367" checksum="C4BE1DE37AA6000E" modified="2023-11-08" version="1">MGAGAFIEPLVVITLLFGGTYVNRNSEYRLLGRRRIYQNSPRSSSPASSSSFDSGRSSPSSSASLLANVDDEPKWRKREIKILGLRKEVISPNTRRFQDYFLSRLLKKFPFLVEAWYWALIYWVYQLGRAFSAVTMVEGTVNVARRHALQVIHLEQHLHIFYELSVQNWFLQHPFLMHWTNRIYSFIHIPGTILFLVWLFYYTTTRNRLDLPLHNKPLGEADGSPAGPKLYAARRRTMAVCNLIAFIVFTLWPCMPPRLLSDANVPGAVGKEARSYGFVDTVHGAEGESSVWTQNKFCNQYAAMPSLHFGYSLLIGLTVMSIPLAPQHRRSRSVPLTMGLRMRVPSARRLLCITLGVAYPAVILVAIVATANHFILDAVAGAMVCGLAWTGNRVLLNLLPLEDYFLWCVRIHKPERRVAFAEGDDWAEDRYGRERYGAKGVVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH24</accession>
    <name>A0A9W9CH24_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7704 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010756</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH24"/>
    <dbReference type="OrthoDB" id="5313995at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056121">
        <property type="entry name" value="DUF7704"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37019">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37019:SF1">
        <property type="entry name" value="EXPERA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24803">
        <property type="entry name" value="DUF7704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7704" evidence="2">
        <location>
            <begin position="8"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4361815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="159" mass="17586" checksum="CD3E5EDDD064FC18" modified="2023-11-08" version="1">MASAASARVPAFYRFVFLWLEPISILTGAVYAHFFPSIYLDLTHSASAPGTTIPVSTSIIMTQLANLYLGLAILEATVLRSTSHVNVWSTFIIGLLIADFGHLYSVRAAGSDVYWAWWSWNAIDWGNVPFVYFLAVTRILMLFGVGFPSHNAGARLKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHZ6</accession>
    <name>A0A9W9CHZ6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009770</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHZ6"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF2">
        <property type="entry name" value="ALPHA_BETA-HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="146"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4363476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="389" mass="44109" checksum="F93A8C05BA373A47" modified="2023-11-08" version="1">MILSQISYLDCIAFLIFLTPQLLIQIGVIPILTWLLPALPHLALVIPYQFVRERFFIPHEHRTLFVKKATPFQDLVIRFVRYAFANMPAFLGRVFFSKAVSLPFLRFRMLRHGIVTSPIRWTEITRPNFKGVWIVHDQQEQPDIIVYYCHGGGFSMGSSFFYMEFLLAWVSLLRSAGYRNPALFALEYTLVPDATYPTQLQETLAGYEYVLSIAQASSRIVVGGDSAGATLILSFLLYMTDQIELRHQRPGLAIMISPWVTIVSRNNRNTASDYLNSSSLELYGRQYIGSKVAANDSLVSPGHCKDVKKWTDASPEKGWYFLYGSEEVLGPETRDLIRLLSSTGKDVESWEDKGGIHAWPVASLYLGATREERLGGLKSIVEATREIIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI14</accession>
    <name>A0A9W9CI14_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009291</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI14"/>
    <dbReference type="OrthoDB" id="3177213at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010640">
        <property type="entry name" value="Low_temperature_requirement_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42101">
        <property type="entry name" value="CHROMOSOME 16, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42101:SF1">
        <property type="entry name" value="LOW TEMPERATURE REQUIREMENT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06772">
        <property type="entry name" value="LtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="403"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="433"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="467"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="496"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="530"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="667" mass="74787" checksum="453F276E6E5D0302" modified="2023-11-08" version="1">MYQHTDAQSLINYLKLFTLMWFTWLSTTLFDVRFSIDCVWNRVHKAIQFGVFAGFVFAGPVFDKYNNSSDSRSYRNFAIVLVVSRVAIAIQYFVVLWQGRMFRQTLIPLGLSTTVHALAAVGYAITLIVFPKGAVGLEEQITWFIISIVEGLSIFLIAMVWRILSFKYTHLVERLQLLTLIIIGEGIIGMVKSIACITKGQADNNAKEIGTVASAIVLLYILWMLYFDQFSSDRFGTVRQQIWSILHYPLHMAILLCVEGNTSLIVWNSAVQALKWMWSLEPSNYSDPAAGFNNTSDYLSFLNTSMYNIDSRFKSKYWNTTYDWGRNFTAIENYTATFGYRTEEWNNRTGDVIRYLFESAQVFVFEAHADSLAKLNAVTAPSSGPRYKLDRIFDVFNVTVMQFYIGAGAMLLILAMMYWFNKLHKTKYEFGEMINRVVVGFTLIIVGVAAVIGDKTTGGFKFSLSEWVIAIVVLLFVAILVSDNALLAVSHHTSRRSSHHRGGSWGGSTVNDTSDENDTVNLLSRSPRNVPSPSPNPPTAPFHAVHTAYGPYDSPPAQDQYLAAHTTNNSTRARSRSPYNTSRVTLHEDVTGSGHLDFSYQNTPVEALGIGAAVSPMSQREGDAASSNYSSRAGYESLGVVDEDEYDGREYVQHPLRVRTVKEGGTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIG7</accession>
    <name>A0A9W9CIG7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_009583</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIG7"/>
    <dbReference type="OrthoDB" id="1277691at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF112">
        <property type="entry name" value="GROWTH HORMONE-INDUCIBLE TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="236"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="274"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4364128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="340" mass="37329" checksum="51068BD50838616A" modified="2023-11-08" version="1">MAFLLRRPFAITATLKQASPKSSPTLTLRAFHNTPLKQHPHFFRPAKPAASTSQNVSIFRQTFRRRYQQAAYNPVAQADFRQRALYGAGIFGATLLGINFIFNRETREDGGMPPFERAYLNDTFMHTGLGIGMIGIAARALHMNGWSFRLMGANPWLVLGVGLVGSIGTMYGTMATSPSNYVQKYALWSAFNVTQALLLSPLFFMHPAILARAGLYTVGMMGSIAFVGATAKTDKYLYLGGPLLAGVAIVALSGLAPLVVPATAARTLMFTENIWLYGGLAVFGGFTLYDVQKVLNHARQAERGLIPKDPVNEAISLELDFINIFIRMVQILGMSQNRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJK8</accession>
    <name>A0A9W9CJK8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 19">Chromatin-remodeling ATPase INO80</fullName>
            <ecNumber evidence="19">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">INO80</name>
        <name evidence="24" type="ORF">N0V83_007600</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">ATPase component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair. Its ability to induce transcription of some phosphate-responsive genes is modulated by inositol polyphosphates. The INO80 complex is involved in DNA repair by associating with 'Ser-129' phosphorylated H2A histones as a response to DNA damage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 19">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Component of the INO80 chromatin-remodeling complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Component of the chromatin-remodeling INO80 complex, at least composed of ARP4, ARP5, ARP8, RVB1, RVB2, TAF14, NHP10, IES1, IES3, IES4, IES6, ACT1, IES2, IES5 and INO80.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 19">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="19">The DBINO region is involved in binding to DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="19"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="372624at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18002">
        <property type="entry name" value="DEXQc_INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000022">
        <property type="entry name" value="Blast:Putative DNA helicase Ino80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001445">
        <property type="entry name" value="Chromatin-remodeling ATPase INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001269">
        <property type="entry name" value="SNF2 family helicase/ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020838">
        <property type="entry name" value="DBINO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031047">
        <property type="entry name" value="DEXQc_INO80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF2">
        <property type="entry name" value="CHROMATIN-REMODELING ATPASE INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13892">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51413">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0010">Activator</keyword>
    <keyword evidence="8 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 19" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14 19" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11 19" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="24" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DBINO" evidence="23">
        <location>
            <begin position="553"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="21">
        <location>
            <begin position="803"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="22">
        <location>
            <begin position="1374"/>
            <end position="1534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="294"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="373"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="621"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="677"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="740"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1560"/>
            <end position="1638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="82"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="155"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="213"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="390"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="414"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="473"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="487"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="506"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="677"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1578"/>
            <end position="1598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="1614"/>
            <end position="1628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00059666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00065648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU368001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ4367070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1638" mass="185323" checksum="F52BC061A251DC52" modified="2023-11-08" version="1">MPPGHQGEPPPAYHRSPTQTGQPYQHHSPAAPAPLPLHHANNFHPHQHHRPPTSPPSTELPPISTALYSRDTSRYYDPTSDNGERSVGRDPARYDNHYPPQTRDPQANPDTRPPQSPYEKAYQSPVTSSYPHHSPLHRPLSQHRHTGAMEAMSHSPVSPSVYQSLNRGAVQPPPNYARRPSIKDEAPPPTRADPMSLSSIMSSGVDNDPPPKSQQLPSLNADSYQLSKPPPNPLFVKQEADLPPQGNGYPPRGAYESMPPVSSLQPALQFTTREIPVPDEAEVEAALAHIETKQMTEVEEPGNPQEHEEYKQRNQKRALEVANADNSKRKRRRTATLIRFQDVIGAHRDYAKQSYNLEHEGDAWQQVQSQEIAEEKERKKDMQRKRRREKTIQNEEAKRAEALAKAGQAENEEEKERHLLAAQKAERKAKSTSRLLQGNDPSKDIREVTPHGPNMEGGTMSSFQASDDLLAGGKRKGNRGTGRLKKSKEQKQAEKDAAAAGQAAIDRGDDLPLIAPRDESRFDSRGRSHTEDLGPVVLTSYDSSVYRNLYSQIVKDLARKDVPKVVRIKENSLSTKQSNLRKTAQLAAKEARRWQMRTNKNQKDTQARAKRGMREMLAFWKRNERDERESRKNAERQELENAKKAEADREANRQKRKLNFLISQTELYSHFIGKKARTSEIERSTDDADTAAAAPAEEHHTGIDVPHLPTATGGKVTNFEDLDFDNDDESALNAAAMANAQHAIQEAQDRARAFNKPGGEDEDGELNFQNPSGLQNKEDWIPQPGMLNCTLKEYQLKGLNWLVNLYEQGINGILADEMGLGKTVQSISVMAYLAERYNIWGPFLVIAPASTLHNWQQEITRFVPKLNVIPYWGTAKDRKILRKLWDRKHVTYDEGSPFHVVVSSYQLVVQDAQYFQKMRWQYMILDEAQAIKSSQSSRWKSLLGFHSRNRLLLTGTPIQNNMQELWALLHFIMPSLFDSHDEFSEWFSKDIESHAQSNTKLNEDQLRRLHMILKPFMLRRVKKHVQKELGDKIELDVYCDLTYRQRAYYANLRNKISIMDLIEKAVGDEQDSATLMNLVMQFRKVCNHPDLFERADTWSPFSFAYFAETASFLREGQNVQVAYSTRNLIEYSLPRLVCRNGGRLDIAGPENPKAGFKGRYLDNLMNIWSPDNVEQSARQAGAFSWLRFSDLSASEASKIAKSGLFHRALAAVEKPNKAGRFNVFYDKENGEWEPKQSIFNIAERHDRAALTDITGEGYMHNLLNVSQSAFEKTGLNVIEPCAKPAASAPPIELYCASQGVIAEKQRAFFNAPIRNALYGVSDKTELSILDSKTDHTALTLRNKLPPPTNERTRFTHIEAPSMSRFVTDSGKLARLDALLKDLKANDHRVLLYFQMTRMIDLMEEYLTYRNYKYCRLDGSTKLEDRRDTVADFQSDRSIFVFLLSTRAGGLGINLTSADTVIFYDSDWNPTIDSQAMDRAHRLGQTRQVTVYRLITRGTIEERIRKRALQKEEVQRVVISGGGGAGVDFNTRSRENRTKDMAMWLVDDDQAAEIEKKEAELAEQEKNAPAGKKRKSKKKVDVNLDDMYHEGEGHFDESAKASGAATPIPEAAPGGKKKKGLSKKAKTARQRLAVADGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK59</accession>
    <name>A0A9W9CK59_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gfd2/YDR514C-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008028</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK59"/>
    <dbReference type="OrthoDB" id="5953249at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040151">
        <property type="entry name" value="Gfd2/YDR514C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048519">
        <property type="entry name" value="Gfd2/YDR514C-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28083">
        <property type="entry name" value="GOOD FOR FULL DBP5 ACTIVITY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28083:SF1">
        <property type="entry name" value="GOOD FOR FULL DBP5 ACTIVITY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21762">
        <property type="entry name" value="DEDDh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfd2/YDR514C-like C-terminal" evidence="1">
        <location>
            <begin position="184"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="348" mass="38088" checksum="E966A6F54F4002C6" modified="2023-11-08" version="1">MSPPLYISDLLARGNAVQAYNELRAISRVPKSTAPAITEPVTCAANWEASIRFIAEGPNGIHIIRGKGRDKKKNKAVELAYRDALENLVQASDVITDIGCGQHHAATTQPRTLPGPTKNPTEPQARIIARARSHLETANIINKRVTDCHPTRIIVLDVEKHTRTGGLLEVGIVDIMVNSKQLGVEHYIVQEEINEPGSRIARPYEFAYGASRTLSKAIIVTQLNVLIQDLKSMKGVVYLSGHTVCNDIKWLQEEGADFSELPVIDVSEVEKAQRSTHQAISLERLGFQSYNVRIRGDLHNAGNDAVLTAGVLVSQLTALAQDGNQMEWVMQTLDGFADEICPGTLGHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK80</accession>
    <name>A0A9W9CK80_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Cutinase</fullName>
            <ecNumber evidence="3 12">3.1.1.74</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_008088</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the hydrolysis of complex carboxylic polyesters found in the cell wall of plants. Degrades cutin, a macromolecule that forms the structure of the plant cuticle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>cutin + H2O = cutin monomers.</text>
            <dbReference type="EC" id="3.1.1.74"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cutinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.74" evidence="3 12"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK80"/>
    <dbReference type="OrthoDB" id="2975078at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050525">
        <property type="term" value="F:cutinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000675">
        <property type="entry name" value="Cutinase/axe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043580">
        <property type="entry name" value="CUTINASE_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011150">
        <property type="entry name" value="Cutinase_monf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48250:SF2">
        <property type="entry name" value="CUTINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48250">
        <property type="entry name" value="CUTINASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01083">
        <property type="entry name" value="Cutinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00129">
        <property type="entry name" value="CUTINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01110">
        <property type="entry name" value="Cutinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00155">
        <property type="entry name" value="CUTINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041020187" description="Cutinase" evidence="12">
        <location>
            <begin position="21"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="78"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="215"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR611150-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR611150-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4366452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="250" mass="26115" checksum="BDC92CA46E20F37E" modified="2023-11-08" version="1">MKGFVHSVLPLAVFFTAALSAPTPSNGPSFPITDLIGTDLTIEEYAQQLEGQSTEEPALTKRQYGGNTYNQLTDGTPCREITLIYARGTTQDGNVGAATDEGPTFFNALASRLGGTSRLAIQGVTYPANVLGFLAGGDAAGATTMFNLINTAASKCPSTKIVVSGYSQGAQLVHTATQRLSAASAARVNAVVTFGDADRDETFGLVPTSKVLIICHTGDNICDNGIIITPEHRDYEKDAPTAAAFVVAHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKD7</accession>
    <name>A0A9W9CKD7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_007422</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKD7"/>
    <dbReference type="OrthoDB" id="424974at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF77">
        <property type="entry name" value="TRANSCRIPTION FACTOR, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12940)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="29"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="104"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="636"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="640"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4366893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="781" mass="87382" checksum="B1CE8033259EF420" modified="2023-11-08" version="1">MVETKFTAQPQSPTSAARPSSGRQRVISSCLTCRRRKVRCDHTHPVCSSCTRGNHVCTWTDQVQVSTGIGRISKPLLASNGKIAKNSDVQARLDRLELLLEKAVAGQGTNPPTSVRSSSDFERKPDVEAQLTPSTNSQTSHGGGMASDDGDGTLLLDGGQSQFVSSLHYALLADEIQDIKALLGDKTDEERKEVPQSSLVDLLSLGRAGAGSNLEQLLPSTQEHRDTLLDVYFANVDPMVRIMHRPTVLRKFSSYNREAHPIAFAIFFSAINSLPPKVVQNKFGESKDGLLDRFQLGVEISLARENYLTTSSLEIFQGFVLWLTCITKEEDMGKAWVLLGIAVRIALNQGLHRDPSLFPTGSMDAVTIELRRRMWHQLGHLEYRAAECKGQEPSISEDFYTTLFPRNIDDEELIDGASPSPAPYDEEKFTSMSFQLVRYMGMRALRKIIQSTYRLERRMLDSGLHGASRPDFARELQNLYEQIKVMLDEVHEENQRKYLRYLNPEVPMQRLTLGLASLLEWRCYLLFWLRMPRAYRDVVFSNDIRRSIFEKSVNCIETLNGAAADVHAARFQWHIGSIASFQAIMHVLSELRNPLFDAPDRQRALSALQMARILRENNGAKAWQAVKNMIDKAVAEHNASPRSQSQSSSTLASPTAPVSSSIRMDNPSNGNIPVYPVLGTIPSYALSTSSGQYVPHNLPAQQQNMPPQAELLQPMQSMQDQGAPCWDDINLSNINNIVGDVEQNVGIIPDFDFGFWGDPFNYENEPAAMPMQDSYFPQWTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKV0</accession>
    <name>A0A9W9CKV0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_006530</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKV0"/>
    <dbReference type="OrthoDB" id="1939479at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF141">
        <property type="entry name" value="GH15225P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="603"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="195"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="282"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="422"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="200"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="286"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="344"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="362"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="436"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="460"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="481"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4368174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="818" mass="90387" checksum="CC97F9B843BB2634" modified="2023-11-08" version="1">MSKRTFDSAFTAMPGGHVPGQWPLESSLLVERGGAPASTSSLFTTIGRWAATVSMVANLFTLPVRLAQRFLRKQTIRAVPVIREDGSNKKRLVDAGREDELATPSPSVRARANLARRRFQPATTPFSPDLWAPTPSPSPYQPSEILEVASPLTDDSYDDESLQFSFTNVLTSTPPYNPNTGSPVPISWDSPSILAPVGSPSPSTPSRSPASSVDGSAIYESPIHPIAFKKDSPSIRRPLGSPSRRPAKKVSPMRNQLLKTQLRVKIERQAALEAQAALDARKAQEAQDDADAQEESIAEAEAEAQTLEWEHAVKNDSDLPIASSSPFQTDVSSSTFQSDIDSSPFQSDIGSSPFQSDVGPSPTESKVDSATSNPPFEYENDLSYLSDAPSPPPKKCVQWTQHAVAKPFYFDEKVGEMLDSTLETITSSPVRVWEAAESEDAAESEDTSEHEIQRAPLNSEDSEDSKDSDDGQDSEAEASDEGYHDNQKGSKKTVTNEEVQKTVSHEEDVQKLVAHESIGFRGVPEDTFDCSEDSLEESQLSIELLEELQEDLRQKLALEPPPPPPRPVQPLVQPLSLDELTALATVVNTTDNGRNATMWVVEPKLYARDLGTLLPDLFNGDIRAWLNDNIVNEYLTILVDHVKQDVGFVHRRGGPAPPCHAFSSFWYSTLKSRPESIGRWAAKFGLKGRQYLDAHLLLYPICDEGHWRLLAVKPKERTIEYLDSLGFSGKKYIDKLKGYLATELEDAWVEEEWTIVELQRSIRQLNGSDCGVFTLLNALTLIRNEEYRRVEACFGMPDARKRIAITLLAGHPTTEFDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-04-02" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL58</accession>
    <name>A0A9W9CL58_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3825435at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="735"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1540"/>
            <end position="1633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="342"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="745"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="790"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="800"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="817"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1548"/>
            <end position="1564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1565"/>
            <end position="1575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1576"/>
            <end position="1587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1619"/>
            <end position="1633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1711" mass="190243" checksum="3C6C1B8BF274182F" modified="2023-11-08" version="1">MPPKRKATNSGGGSSKPAKKQKASKDAQPPHADDEPRNASPDAPGRTEVRWGLPATARDNNAPDTSNEITRFAPALYNQDWDELTTHETAFGFPRLRTPLYKTRTDKEYVPPGNNISQTSQAVLDFFHVIKGESAPQKKGTTLGPYARSAFENAFGDEDDDFAVVASRSGDHPVRALDQDYRLAVTVRDGDREASAHVDLIRRATPTPAATGHAYFIDLPAGTNVTINGHTYVNDREAAGETATTSPFIIGPFTGYTVIELLSQPVFFFRGREDLNLLGPVVSGARARPSPEELENRDQNGDVPVENTTVTWTAVSTAAPASPTSTRGSKTSKGRSTRNRSTRSGSTRSGSTPAPQIVSVNPRVTHTSSRNAPQASPKGPTTVEDESAGSDTISTPPSSGANSPVPERATYDDTWTDQGEPITNPCDYLSRHLLSPHEHRYPELIPRVLASINDRQFSGAGFSFQHNNRILRPGRTLIAPLNQNGHHVLLVLQLQPEFAGITVRILDPMAWKSTLAERDKFVELALALLEDGEWWQETYESLTELNHDLPDAFEWVPCAQHTASEQSVVFTVLHAWAVALGLELDPAFRLGRDESVSFFTRAQRLFDLALRDHLDWKILLAFFRCTGFVETGDEEAENSDESLPPHDRRFDQRVRGFQSSLERQENPDVTLGDASFGADFSFVISTVEVVLEQGVAHTKTFASDDWDEAKLEDLASLVQDGSWSLADTQEQLIERVSQRENGDESVSTPTSSKKSSGTFSSLTKSSSRREDKTSGSKPPPGPVSATVPDLDQDPAEDDSNASANGEDTASSSTSPPPDHDLPKDYDPCVDFRQRMDGLLREHDADLRDLRDRERNTVTTEYGEWLDDTEVSLAIASVTLAISELQGIDGGFGFFHPISIQQCAADTYPLPISPTLRPGRPLLLPIHIQAHYILLVIQLDEQGQTSFSVLDSKAYHLNMRHRQSVHDLAQRIVKRSDWNRHVTGGEDLIHHSPPHTTWIPVSQQPSDNECGYYTILNAWTLALGLLPNPNIHHDWNDQFFSDVNNLVHLARLGRADWKLIYSFLRCRGFANDGHVPRDRQFTATRELRNEGEYLETALDDMHALEEVHFMGHPALDRQQLKDINRMALPVGRRHNDNATFRSDQWSNDDRVNYASDLARYGKLDLNFTRANLRSAHTIVRDIRGLHLRKELGDNIPNYQQLPRAQLLDATRRYLAGWHTGRDLFLREDGAKGTANLCQFFEDTKQFLKYIFFSPNCVGEKLKGGLFERHTVLMRDGGLEKAKNGKYTRAGDLMLDDSDMNMAIAAVVDAIDTMQSPTDSESIFAGGFSLATSVQLQMGLAAISGDNLLPALDAWRKKNRVPTKSQGQAGGHHLLAVVQEQRKKNPHFEVTLYDSATHVFGNAYPFLNTAVVRAATALKWSTHRNPDYRVKFATTPRTAAVAQQPTGGWQCGPHVVINAWILAMGLHPAPNAKFTDEVYSEFYTLARAACAGLLDWRTLATWFFCRKLTLQTGLEAVPQNRRFTITQFWENEEELTIMWGVLPPGAEVTDNGKAKGKADEEEKEVYPSEDESDDDVVEAGGDSGSSADAQGDTDEEGEALTRAESDQELMVSGGEDEEAASTDSSSLPTLPSSPEALTRAYEEEPMPSFLGRRRSHHIETPPKHVKRDDNMYFLDAFDDMDDEMRDVVVPVEQQWTRRSSEYRTDRLFFLDAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLH3</accession>
    <name>A0A9W9CLH3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_005932</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLH3"/>
    <dbReference type="OrthoDB" id="6380398at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000077">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040964250" description="Peptidase S1 domain-containing protein" evidence="6">
        <location>
            <begin position="19"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="31"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="257" mass="25767" checksum="3A423A144C4C44EB" modified="2023-11-08" version="1">MLFKDVLIALAIPAICVAAPTANPAKDSENIVGGGASTQGNFPFIVSLQKNGAHFCGGSLLNANTVVTAAHCAVGQTASTLKVRAGSLNRNSGGTLVGVSSIVVNPGYVSSTYDADVAIFKLSTSIPTSSTISYATLPASGSDPAAGTTSTVAGWGTLTEGGSTLPTTLQQVDVPIVSRTTCRADYGQSAITTNMICAGLTAGGKDSCQGDSGGPLINTSTRVLIGLVSWGDGCAEPNKPGVYTRVAAVLSFINAHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLJ3</accession>
    <name>A0A9W9CLJ3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006338</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLJ3"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="197" mass="22575" checksum="F3129A8950C65686" modified="2023-11-08" version="1">MYREATLIAQIKKTTEERVARAEAEKSAALELVAQLQSTSASNKTVMEQHLKLQPAPEDKAKNTDERARRSEEEKQELEILVKKLRGTIDELQAGKTSAEAEGLQLRRQLDIAQGTNSLLEEQKQKLQAIIDEYSGREMTWREDRATIDSYALLVGSLDKRLARAFQCRKECDEACEVFPKQYEDDLEEVRQAIYEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLS4</accession>
    <name>A0A9W9CLS4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_006128</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLS4"/>
    <dbReference type="OrthoDB" id="3886144at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF47">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="62"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="98"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="304"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4369046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="793" mass="88178" checksum="98182071F71DA4CB" modified="2023-11-08" version="1">MAETVTVPSPSLMLKAPGPTPPILATGKTIAPPPPLSASKQAPKASQGNAVTKRKQSKSRNGCITCKAKRLKCDETKPTCQQCARRAVTCGGYKKDFKWRPFEEPSIPGKPPGKPRKDVPSIPTSAPSNFHHHASIPEPQYAFSNPAQPIYYAPPPSSLPIMHDQPLQTSFPPASFPPDPMYAVPQSSPFMVAPPSISPVESYAPSSFRGPQSLFREDSVHTRYTKTTAPSSVSSGQSPRLVDLLLPGTDLTVPPEEYSLFLSQHEAFYQPTGLTPPPPVDDDIEEIPRSVAIDPEAWVMRLPSPAASSSSSSSSDSPDFKILSQPQFSLTSPETLTRRYDRDTCGVLSVKDGPTENPWRTLVWPLARDCPALYHAIASMTSFHQSTEMPAMRIQGIDHMRNSVHALAAGLQDMRFDAAISTTLVLAFSESWDQHISTGIDHIKGAKILINQALVQHRQTPKQGEEWTRLKFLCNTWIYMDVIARLTSADDDESNDVDAVHESIYATGETETTLDPLMGCANTLFPIIGRVANLVRRVRRTGSNGPSIISQAVQLKSQIEEWTPPSFIEDPEDETTSPHDSIKTAVAYQYATLLYLHQAVPEIPSQPSSVLAKRVLCELATVEPRSRSVIVHIYPLMAAGCEAMDQEDRDWVKERWGFMSSRMKLGILEKCLEVTKEVWSRRDAYEAERMDFDSMRNHDFSTDIPLKRDFESFFDDYDIEGEFCWLDAGPKRRALDDKLAYAVPLSSPVVGEPTRRKSPSNSNTENLEYEFTVKGRLHWLGVMKDWNWEVLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLU7</accession>
    <name>A0A9W9CLU7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Actin-related protein 2/3 complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ARC19</name>
        <name evidence="7" type="ORF">N0V83_006039</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Cytoskeleton</location>
            <location evidence="6">Actin patch</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ARPC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLU7"/>
    <dbReference type="OrthoDB" id="336240at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1460.20:FF:000001">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034666">
        <property type="entry name" value="ARPC2/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008384">
        <property type="entry name" value="ARPC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22629:SF0">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22629">
        <property type="entry name" value="ARP2/3 COMPLEX 20 KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05856">
        <property type="entry name" value="ARPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039100">
        <property type="entry name" value="ARPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69645">
        <property type="entry name" value="Arp2/3 complex subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR039100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4368957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="169" mass="19623" checksum="7F4A6B568B67859C" modified="2023-11-08" version="1">MSQSLRPYLQCVRSSLTAALSLSNFASQASERHNVPEIEAASSPEVILNPLTVSRNENERVYIEPSINSVRISIKIKQADEIEHILVHKFTRFLTQRAESFYILRRKPVKGYDISFLITNFHTEEMLKHKLVDFIIQFMEEVDKEISEMKLFLNARARFVAESFLTPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM59</accession>
    <name>A0A9W9CM59_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_005344</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM59"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000061">
        <property type="entry name" value="MFS sugar transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF68">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="373"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="408"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="440"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="16"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="506"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="507"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="535"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4369582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="553" mass="61046" checksum="7383C0CAB5311856" modified="2023-11-08" version="1">MPRFYGLRGLKLNIAIAIVAGTDFALFGYDQGVMGGLLTLGSFLKYFPQIDTVNPPAGSSPSHAATIQAITVGAYTLGCFFGAVATIWLGNMLGRKKTIFYGSCIMIIGATLQTSSFGLPQLIVGRWITGFGNGMNTSTVPTWQSETSKPHRRGQMVMIEGSLIVFGVMLSYWLDLGFSFLEPSSIAWRFPIGFQIVLAIFILIAIPGLPESPRWLVLKGRNDEALEVLAALSDLPPQDKKIQQEFQEVKDVVFEMSRGGFRECFKLNRNRNFHRTALAYVNQMFQQISGINLVTYYAATIFENSIGLSPFLSRLLSACNGTEYWMASWIAIFTIEKFGRRALMIFGAAGMSISMAILAGATSNIGNKSLGLLATVFLFVFNSFFAVGWLGMTWLYPAEITPLSIRAPANAISTTANWAFNFMVVMVTPPAFANIGFRTYIIFAVINAAMVPSVYFFFPETAGRSLEEMDEIFHKTTNPFNCVKIANELPHRYGKKGELLVEYGDTEEAREAERRRSSVTGAEIRQPTGGVLNGEAEKDVREHHEHGSKSNGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMF5</accession>
    <name>A0A9W9CMF5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">3-keto-steroid reductase</fullName>
            <ecNumber evidence="7">1.1.1.270</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ERG27</name>
        <name evidence="7" type="ORF">N0V83_004691</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the short-chain dehydrogenases/reductases (SDR) family. ERG27 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.270" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMF5"/>
    <dbReference type="OrthoDB" id="9989144at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000253">
        <property type="term" value="F:3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051593">
        <property type="entry name" value="Ergosterol_Biosynth_ERG27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43647:SF1">
        <property type="entry name" value="3-KETO-STEROID REDUCTASE ERG27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43647">
        <property type="entry name" value="DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0752">Steroid biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4371474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="439" mass="49382" checksum="71BF4D1209C0CB42" modified="2023-11-08" version="1">MSYLDDIPTPDNGFYVLVTGANSGLGLGIGTRLIDEFLQTRPQSESLVLIVTTRDQRKGDATIEKLQRHLRKVINGYEQKLPGISQVLQQRVFFRQERLDLLSLISVQKLAKRLRETTPKLDVLICNAGIGGWTGINWPLAIYKILTSWSTAVSYPTFKISGVGWVAKPQIPAEKKAEEPPLGEVFCANFFGHYLLGHYLAPLLAKHPKSEKTRGRIIWVSSLEAYEHTLDMNDIQGIASNIPYEGTKRLTDVIAISSTLPPTSGLVDQYLDHPQTSEKTTKARLYVSHPGICGTSIVALPLILEYLMLAAFYVARWLGSQWHNVTVEKGACAMVWLALAQQSTLDDMEQKEGVGKWGSATDFWGEERVERSEVAGWGWGGKLGEYKRKKGRDPYAEDLTKESQGRFLQTGRKCWEEMERLRIEWEDRLRNAGVGIEMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMP4</accession>
    <name>A0A9W9CMP4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA pol II transcription cofactor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PRN1</name>
        <name evidence="5" type="ORF">N0V83_003795</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the pirin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMP4"/>
    <dbReference type="OrthoDB" id="198735at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02247">
        <property type="entry name" value="cupin_pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02909">
        <property type="entry name" value="cupin_pirin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903:SF8">
        <property type="entry name" value="PIRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903">
        <property type="entry name" value="PIRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05726">
        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin N-terminal" evidence="3">
        <location>
            <begin position="92"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin C-terminal" evidence="4">
        <location>
            <begin position="247"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4372022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="372" mass="41161" checksum="DF70268FD7B7C252" modified="2023-11-08" version="1">MKTLSTIFFALIAIAIAVYRSEFMLSRVTQLSKQITPITRFTSAFYNTTRDMSTSTMFSGLPVIPDEPLTTNAPRKIAKSFLAIEQSEGAGARVRRSVGTPKLRNFSPFLMLDHFAIPPGAGFPDHPHRGQETITYLLSGAVDHEDFAGNKGTIETGDLQFMTAGRGIVHAEMPRQNADGSPNVGMQLWVDLPKELKSCEPRYRDLRAKEIPETTADNGKVHVKVISGKAYETESLKELAYTPVWLLDFTVQPGGTVKQSLPKGWNAFAYTLNGTTIFSSDGVSRPIEQYHNVVFEQGGDSIEASVEEGAEKESRFILVAGLPLDQPIVQYGPFVVTSRDEVMQAMMDYQTHSNGFERANNWESEIGKSMVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMW0</accession>
    <name>A0A9W9CMW0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mannose-1-phosphate guanyltransferase</fullName>
            <ecNumber evidence="4">2.7.7.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">GDP-mannose pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">GTP-mannose-1-phosphate guanylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">MPG1</name>
        <name evidence="17" type="ORF">N0V83_004391</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in cell wall synthesis where it is required for glycosylation. Involved in cell cycle progression through cell-size checkpoint.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>alpha-D-mannose 1-phosphate + GTP + H(+) = GDP-alpha-D-mannose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="EC" id="2.7.7.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.13" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMW0"/>
    <dbReference type="OrthoDB" id="1733332at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004475">
        <property type="term" value="F:mannose-1-phosphate guanylyltransferase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071554">
        <property type="term" value="P:cell wall organization or biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05824">
        <property type="entry name" value="LbH_M1P_guanylylT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06425">
        <property type="entry name" value="M1P_guanylylT_B_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000017">
        <property type="entry name" value="Mannose-1-phosphate guanyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000013">
        <property type="entry name" value="mannose-1-phosphate guanyltransferase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045233">
        <property type="entry name" value="GMPPB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="15">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="16">
        <location>
            <begin position="224"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4371175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="332" mass="36408" checksum="2AE910E907ED4D29" modified="2023-11-08" version="1">MIQHQIEALAAAGVTDVVLAVNYRPEIMAEALKTYEKQYNVTITFSVETEPLGTAGPLKLAEKILGKDESPFFVLNADVTCDYPFKQLAEFHKQHGDEGTIVVTKVEEPSKYGVVVHQPGSASKIDRFVEKPVEFVGNRINAGIYILNPSVLQRIELRPTSIEQETFPAIVKDGKLHSFDLEGFWMDVGQPKDFLSGTCLYLSSLARKNSKLLTPASEPYVYGGNVLIDSSAKIGKNCRIGPNVTIGPNVVIGDGVRLQRCVLLKNSRVKDHAWVKSTIVGWNSTVGKWARLENVTVLGDDVSIGDEVYVNGGSVLPHKSIKQNVDTPSIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMW4</accession>
    <name>A0A9W9CMW4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMW4"/>
    <dbReference type="OrthoDB" id="5402816at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="67" mass="7500" checksum="C98040B3D37BEF2E" modified="2023-11-08" version="1">MAAIDMNLVARSEYALHQLVKRKKNWAQREPGVIVVFVVVFLVALLVIAMFINKKRHAAKAGAVVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNW0</accession>
    <name>A0A9W9CNW0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Nnf1-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_002630</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNW0"/>
    <dbReference type="OrthoDB" id="18453at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000444">
        <property type="term" value="C:MIS12/MIND type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007128">
        <property type="entry name" value="PMF1/Nnf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15459">
        <property type="entry name" value="POLYAMINE-MODULATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15459:SF3">
        <property type="entry name" value="POLYAMINE-MODULATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03980">
        <property type="entry name" value="Nnf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4373891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="24216" checksum="74640AF3976D6A53" modified="2023-11-08" version="1">MPAATKLDSRSPSPTPAPPIADTPGPRAQGLINVFNQASKATLDKCSSKNFASCFPTAAQYSPGVLDNLRGQIVDQLDRTWKTNFDDILERRNVVKLLNSLDQCIEDAKLRKKRAEASANGGPVEAPMPPHTLTPSEIHLAHLMPFLEQQATEMNTRLVETQQANTELLSTVTAQRAEIETLVRGLGNVIQDLEVSAQTMAQDDVQDLSKEIRDLETGMRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPI1</accession>
    <name>A0A9W9CPI1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit</fullName>
            <ecNumber evidence="3">2.4.1.198</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">GlcNAc-PI synthesis protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SPT14</name>
        <name evidence="10" type="ORF">N0V83_003661</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalytic subunit in the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.198" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPI1"/>
    <dbReference type="OrthoDB" id="734129at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000506">
        <property type="term" value="C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017176">
        <property type="term" value="F:phosphatidylinositol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03796">
        <property type="entry name" value="GT4_PIG-A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000053">
        <property type="entry name" value="Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000026">
        <property type="entry name" value="Phosphatidylinositol N-acetylglucosaminyltransferase subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039507">
        <property type="entry name" value="PIG-A/GPI3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013234">
        <property type="entry name" value="PIGA_GPI_anchor_biosynthesis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45871">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45871:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13692">
        <property type="entry name" value="Glyco_trans_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08288">
        <property type="entry name" value="PIGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIGA GPI anchor biosynthesis" evidence="9">
        <location>
            <begin position="21"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4373366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="493" mass="55053" checksum="2DC92E95B360A733" modified="2023-11-08" version="1">MPYNIAYKLIDRGHKVIVITHAYAGRTGVRYLTNGIKVYYVPFFVIYRETTFPTVFSFFPVFRNIVIRERIEIVHGHASLSSLCHEAILHARTMGLRTVFTDHSLFGFADAGSILTNKLLKFTLSDVDHVICVSHTCKENTVLRASLDPLMVSVIPNAVVAENFRPISDDLDSSGGPRHNFAPGPPQPMGPDEIITVIVIQRLYYNKGTDLLVAAIPHILAAHPNVRFIIAGKGPKAIDLEQMVERNVLQDKVLMIGPVRHEEVRDVMVRGHIYLCPSLTEAFGTVIVEAASCGLYVVATRVGGIPEVLPTHMTEFAAPEEDEIVEATGRAIAKLRAGKVRTELFHNQVKQMYSWTDVAQRTERVYDGISGVISQSDFYQSGGAAGAWSATRGHAEVQSFALIDRLKRYYGCGIWAGKLFCVCVIVDYLLYLVLEVFAPRSRVDICRDWPKKMPKERKEGQDHIANAENIGSKILRKGGSRRRREFNGELNDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPI2</accession>
    <name>A0A9W9CPI2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001856</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPI2"/>
    <dbReference type="OrthoDB" id="3801070at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="280"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="82"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="214"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="561" mass="60841" checksum="56ED2AB2814848C3" modified="2023-11-08" version="1">MSSSPFVFGAGANHSFSGVSTSQSSTSAPPAQAAPASVMEDLLRAELDVKMAAVDRLGVELARAERAATVALSAESVARSALNALRNTIKEEKSRMDDYDRVVKLQADLDQVTAEADGLRKEVRELQGQLGTTTANLHSQAQQVAEKDSALEGLRAQVGELKQAVEDEKATTANVASSLENAYADLTTTDNARKSALKEKDDAEAAQHDTTLQFEALHVQWKDAQAKVEELEDQMDAMRQLENENAGLYDDMENLRRQLADHERTLIVKDERISHLETQFQKERQRNLNAADAADAAASTSPIDAPPQPFNSLGDSLEEELANTSDYTDSVYEPLEYSDVVEIADFEPITPAPGLLKYSNVLKIANIEPITPASSLTPLEHSNVLEIADFKPITPASGLTPLESSDVLEIADFQPITPTSGPASTLSIIQGASIAPMPHQLITTSTPMQTDAPPAGLTQTKKPSFFDNVLPSLLALLAFFFITQYAELQAWKTANGAGYRPGYGSTYDRTGAYGNGRYLFNIIPLAMDVGNTWWSEPVARNVATAITRFETWAGIAYTPLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPJ7</accession>
    <name>A0A9W9CPJ7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">NAD(P) transhydrogenase, mitochondrial</fullName>
            <ecNumber evidence="5">7.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Nicotinamide nucleotide transhydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">N0V83_002009</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="27">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane. May play a role in reactive oxygen species (ROS) detoxification in the adrenal gland.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>NAD(+) + NADPH + H(+)(in) = NADH + NADP(+) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:47992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="7.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">In the C-terminal section; belongs to the PNT beta subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the AlaDH/PNT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="27">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.1" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPJ7"/>
    <dbReference type="OrthoDB" id="37244at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008750">
        <property type="term" value="F:proton-translocating NAD(P)+ transhydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05304">
        <property type="entry name" value="Rubrum_tdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000028">
        <property type="entry name" value="NAD(P) transhydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000002">
        <property type="entry name" value="NAD(P) transhydrogenase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008143">
        <property type="entry name" value="Ala_DH/PNT_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008142">
        <property type="entry name" value="AlaDH/PNT_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007886">
        <property type="entry name" value="AlaDH/PNT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007698">
        <property type="entry name" value="AlaDH/PNT_NAD(H)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026255">
        <property type="entry name" value="NADP_transhyd_a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024605">
        <property type="entry name" value="NADP_transhyd_a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034300">
        <property type="entry name" value="PNTB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00561">
        <property type="entry name" value="pntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006942">
        <property type="entry name" value="PRK09424.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10160">
        <property type="entry name" value="NAD(P) TRANSHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10160:SF19">
        <property type="entry name" value="PROTON-TRANSLOCATING NAD(P)(+) TRANSHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01262">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05222">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02233">
        <property type="entry name" value="PNTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12769">
        <property type="entry name" value="PNTB_4TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01002">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01003">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00836">
        <property type="entry name" value="ALADH_PNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00837">
        <property type="entry name" value="ALADH_PNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="13" id="KW-1278">Translocase</keyword>
    <keyword evidence="8 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 24" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="546"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="578"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="606"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="646"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="672"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="697"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="722"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="753"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="785"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="850"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="880"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="904"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase N-terminal" evidence="26">
        <location>
            <begin position="117"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase NAD(H)-binding" evidence="25">
        <location>
            <begin position="265"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00054910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00061558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00074145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00079255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="SMART" id="SM01002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="SMART" id="SM01003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="KAJ4374931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1254" mass="132437" checksum="B52EE5AD6D343AFE" modified="2023-11-08" version="1">MSSSASNCLAGYRALASRPPRGSHSSLSANLKLYASAFVLRSTLVIQHEYHSKHAPQQPVARLRSLTRPRPLYLQQLSKVQTTAVARYASHTAEPHDEEIPTSPPFSAGPYSNLTIGVPKESYPGERRVAITPQNVKLLLKKGFSRILIERGAGVEAQFTDEAYEQAGAKTVDRRNVFSESDILLKVRALSIDGQESEVDAIREGATVISFIYPVQNKQVVDRIASRKATSFAMDMVPRISRAQVFDALSSMANIAGYKAVLEASNHFGRFLTGQVTAAGKIPPCKVLVIGAGVAGLSAIATARRMGAIVRGFDTRPAAREQVQSLGAEFIEVDIQEDGSGAGGYAKVMSKEFIEAEMKLFYEQCREVDIVITTALIPGKPAPKLITKTMLGAMKPGSVVVDLAAEAGGNCEATVPGKLAKYDGVSVIGYTDLPSRLPTQSSTLYSNNVTKLLLSLIPKDKREKYYDVDLTDEVTRGVIVTYDGKILPPAPRPAPPPAQAKPAPSPAEQVANIVALTPWQEQSRQVAGVTAAMTTALALGKVTGPLFMSNAFTFALASLIGYRVVWGVAPALHSPLMSVTNAISGMVGIGGLFVMGGGFLPETIPQTLGALSVLLASVNVSGGFVITKRMLDMFRRPTDPKEYPWLYAIPAALFGGGYIAAASTGMAGLVQAGYLVSSVLCVTSLSGLASQTTARRGNFLGILGVFSGILASLAAVGFAPDVLTQFAGLATVGAVAGMIIGRRITPTSLPQTVAALHSVVGLAAVLTSIGSVLAHSGDISTLHMVSAYLGVLIGGVTFTGSIVAFLKLAAKMSSKPIALPGRHLINSTLLGANVATMGAFLAYAPGAPLIGAACLVGNAALSFIKGYTTTAAIGGADMPVVITVLNAYSGFALVAEGFMLDNSLLTTVGALIGVSGSILSYIMCVAMNRSLTNVLFGGIAQTPQAQTKIEGEVTKATADETAEALVNAENVIIVVGYGMAVAKAQYAISDFVNSLRSKGINVRFAIHPVAGRMPGQCNVLLAEASVPYDIVLEMDEINDDFPETDVTLVIGANDTVNPIALEPGSAIAGMPVLHAWKSKHVIIMKRGMASGYADVPNPMFYMENTKMLFGDANDSCNAIKRALEEKIKGFPEPNQRNDIKMSFPLIFTLPPSNRHEIILLDTSSVSTLKSLNKLITSTISASPNCAEFMAKHKAADAPTETIQEFKIHWDNKGRDAKIWPEYTVLTDENLGAVCEVFKANPTAGVLEIKVGKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ27</accession>
    <name>A0A9W9CQ27_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bacteriophage T5 Orf172 DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002466</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ27"/>
    <dbReference type="OrthoDB" id="3692364at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053006">
        <property type="entry name" value="Meiosis_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018306">
        <property type="entry name" value="Phage_T5_Orf172_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28094:SF2">
        <property type="entry name" value="BACTERIOPHAGE T5 ORF172 DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28094">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 113 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10544">
        <property type="entry name" value="T5orf172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00974">
        <property type="entry name" value="T5orf172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacteriophage T5 Orf172 DNA-binding" evidence="2">
        <location>
            <begin position="207"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="440" mass="49899" checksum="3A68D1C5B150C5E2" modified="2023-11-08" version="1">MGRKSSLAAPLPLSNSTSSRRRSANGRLDNPVSTPETPASTESRVRGSSLYLPITISDGEVEEEKTPDRRKSRTQTTQGDASPPRPTSAKVPRSRQQTTPDYARIAPRRDVATDPGKLSTPRLKEYDDKAIPYRPATVVHQDGSEDEREKNGNIIDESYSDKDIKQNGTDKGKESSAQRVHAKLLKQARKQLTKGEEEGHIYIFCDPKKRGILKIGRTKHVDERFKRHMKCGLQVEEVYISHLVQHVKRAESLVKIDLSHLCRPWQCFRCSEEHGEWFKVGPAKAKEVVNQWVTWINKQNPYGNDKNMKPIWNDLLSHGRQPEEVFEHQDHAARWAHWAWALSPPSSKERKLFDKLNDRGQHVNEKTQMTPKKHDNDGKKIIPYRQPPSTEQTAPLTLTDLAKALVSKDQTVHIHITGNVNITTGCKIIGDASVRRLLQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ41</accession>
    <name>A0A9W9CQ41_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Asl1-like glycosyl hydrolase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ41"/>
    <dbReference type="OrthoDB" id="5985073at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071966">
        <property type="term" value="P:fungal-type cell wall polysaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053183">
        <property type="entry name" value="ASL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024655">
        <property type="entry name" value="Asl1_glyco_hydro_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34154">
        <property type="entry name" value="ALKALI-SENSITIVE LINKAGE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34154:SF10">
        <property type="entry name" value="ASL1-LIKE GLYCOSYL HYDROLASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11790">
        <property type="entry name" value="Glyco_hydro_cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Asl1-like glycosyl hydrolase catalytic" evidence="1">
        <location>
            <begin position="37"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="215" mass="23765" checksum="F809F7AEF5170F99" modified="2023-11-08" version="1">MALLAAFISLANAIPMSYNNTIFPRVDPEIPAHERRGIAFNNPDFVHHFAVQPSAGGACMSVADAVAGWNEHIQPLKSLKKQMYLGSPAVSNAAATDSTGLGWLAKFIEACSGCDVDFINIHWYDRADNAAYFKQHIEDARKVAQGRPIWVTEFRAEGTDEQVKRFFDEVLPWMDGSSDVARYAYFMARPGPGMLINDAQDGLSDLGAYYSFHDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR07</accession>
    <name>A0A9W9CR07_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000276</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR07"/>
    <dbReference type="OrthoDB" id="4708870at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="384" mass="44051" checksum="98E68194329E6440" modified="2023-11-08" version="1">MGGLAFANVVTGNGKPPQVPRMSPELYRALSAEYQTRLEKLFEHVVIPREAPSKVDHGDIDFLVEGIRPDASESDIGTTIKDVIGAELHLPRGGSHSYAIPHPQLPDAYVQVDVELSPGDGTPDGPELFEWTRFMKGDSDLLQIVGVSHRPLGLTCNDRGLHVRVEQIEPYNKKKALLFLTRDPNEAMEFYGLDMDKYWVGFTDEEDLFDWASSGRFFSDDIFEGRVEKSNDRSRQVKRPMYRRFVEDYMATKPDQGNTNAWTREEVLQEALTFFNKHTDYSAMMEEHRNKEAEEELWKDIRATIPVEGSSLALALKGLRRWVVFQEGEPRITSEPNLEEYQRWVTFISVETKPKILDWVKKNWEEVKALEKARARAVKESKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR08</accession>
    <name>A0A9W9CR08_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001217</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR08"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="499"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="G" evidence="3">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="481"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4375938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="528" mass="61599" checksum="E56B3C000EDCB677" modified="2023-11-08" version="1">MGITGSGKSTFISKLVGDEVRVGHQLQSCTAEVFPYAFMYSPQQRVYLLDTPGFDDTNRSDTDVLKDVATFLSRMYAGNIKLAGIIYLHRITDNRMGGSAMRNLRMFKKLCGDKYFQNVILATTMWERLQTQVQGEQNEAQLLAERDWWGGMHEQGSKVFRHMDTPESARSIIGYLMHTRPAALRIQEEMVDEHRNLVDTSAGREVEKDLQELREKYVSEIQSLREEQLEALQERDEFMAAELRQQEIKMDEKLRAADKAREDLKIDLERLTQESEQKYSRMIRDLREETKAANEARRKAERDMHEQQQRIAAEAKRERQKQEEDAKRARESQERERRKWQDDMAAMNQRYEDQARKEREQRDEDRRKWHEDLHARDRRIEAQAASERQQQERERQRWQDSQSRLQREADDRHRQAQVETERLRNEITDLRISSRASSPVDYDSPPLDDDDDLSPSPVSSDDDDEDEDDDDEDEDDEDEDDHRPVQRADTRKFGTGASLLIAMAGVVTLNPVVAMAGIGGAILSSQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR61</accession>
    <name>A0A9W9CR61_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Serine/threonine-protein kinase SSN3</fullName>
            <ecNumber evidence="6">2.7.11.22</ecNumber>
            <ecNumber evidence="5">2.7.11.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Cyclin-dependent kinase 8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Serine/threonine-protein kinase ssn3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">SSN3</name>
        <name evidence="27" type="ORF">N0V83_001355</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="27">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the SRB8-11 complex, a regulatory module of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="27">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="6"/>
    <dbReference type="EC" id="2.7.11.23" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR61"/>
    <dbReference type="OrthoDB" id="6284126at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07842">
        <property type="entry name" value="STKc_CDK8_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000408">
        <property type="entry name" value="Serine/threonine-protein kinase SSN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000426">
        <property type="entry name" value="Serine/threonine-protein kinase ssn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF495">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0010">Activator</keyword>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 27" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="17" id="KW-0804">Transcription</keyword>
    <keyword evidence="15" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9 27" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="15"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="367"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00039418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00040708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00041823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="KAJ4376074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="404" mass="45793" checksum="52D92975547E4402" modified="2023-11-08" version="1">MFTGYNTKKRVNERYKIVGFISSGTYGRVYKAEGKNGFTGEFAIKKFKPDKEGELQYSGISQSAIREMALCTELAHPNVVHTVEIILEDKCIFIVFEYAEHDLLQIIHHHNQPQRQAIPARTIKSILYQLLQGLVYLHRNWVMHRDLKPANIMVTSSGKVKIGDLGLARLFYKPLQSLFSGDKVVVTIWYRAPELLLGSRHYTPAVDLWAVGCIFAELLSLRPIFKGEEAKMDSKKTVPFQRNQMQKIVEIMGMPSKDRWPLLTVMPEYTQLLSLNAGNAARFPRPQGGDGLERWYHQTLNNNQYPIGNGPDTPGPEGLSLLKQLLEYDPQKRLTAEKALLHPYFTVHGKPSGGCFEDSKIKYPVRRVSQEDNDIRTSSLPGTKRSGLPDDSLIGRPAKRLKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRA6</accession>
    <name>A0A9W9CRA6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001356</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRA6"/>
    <dbReference type="OrthoDB" id="337038at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05380">
        <property type="entry name" value="CAP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040853438" description="SCP domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="2">
        <location>
            <begin position="40"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4376075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="211" mass="23886" checksum="9483C4BF3F555116" modified="2023-11-08" version="1">MRLLAPFAALTAATAVAAPAHHVHPRELVADDAPHQDDPNFISAVMRAHWYWRQLHCAQELVWDADLADAARADIEQCTHEPQHMRPGSNLSSVKPAPASYDDWVEFARTASHGWHEEETKYPYDNPHYDSSWGHFSQMVWRNSTRLGCALGHCSDEVDYPGRFYCYYSFFGNNIAAGQFEAQVWPPVCGDPTVSEAKKREDVAFNWAVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRQ8</accession>
    <name>A0A9W9CRQ8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_000158</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRQ8"/>
    <dbReference type="OrthoDB" id="5840532at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF133">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4377333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="273" mass="28660" checksum="0B7ABAB5491B0BFE" modified="2023-11-08" version="1">MDVPGFALVTGAASGIGKACALAFAKEGCAGVALLDLNADALSSVRDEIQSICSKAANDKPTCRIEIFPTDVTNEEQVSANVQAVASLFGRIDYVVNAAGIALKHAGGAAYATSESWKRVLDVNLNGTFYVLRESARIMLSQTPIVSSLTSRPLQRGSIVNFSSIQGVVGITLSTAYTASKHGILGLTRSASEDYAKDGLRINAVCPGYTETPMTTRNPDVLKAMEERVQSAVPMQRMGRPEEIADAVLYLAGGRSSFVTGSALAVDGGYTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRW2</accession>
    <name>A0A9W9CRW2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Anaphase-promoting complex subunit Cut9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cut9</name>
        <name evidence="9" type="ORF">N0V83_000323</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRW2"/>
    <dbReference type="OrthoDB" id="10006270at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF9">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="332"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="434"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="116"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="568"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="612"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="128"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="618"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4377498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="655" mass="72826" checksum="EC20123A11C50435" modified="2023-11-08" version="1">MDKFLRDWRQDALNRNQYEAAIFAADKLVAITGDEQDTFWLAQVHFTTGNYTRTQALLTRRGLVERNPQCRYLSAHCAIKLGKTEEALQILGDKNPTHLISAPGSARTKLRHVDVNTRAGPRHNKTGSRNERVPTSEERDREQIDNIKLEAGMCYLRGVCYAKQNSFDRAKECYKTAVQIDVQCFEAFDALMANSLMSPEEEWTFLESLNFDTINVSNNTSLSQEAAQFTKLLYTTRLSKYTKPEEFANASETLSTHYNLNDNPDIVLSKADLMYTHCRFREALALTSSVLIIDKYNFNILPVHLACLHELGEKNTLFLLAHDLADSNPSEPCSWLAVGIYYLSINRIAEARRFFSKASMMDPHFGPAWIGFAHTFAAEGEHDQAISAYSTAARLFQGTHLPQLYLGMQNLQLSNLSLAREYLKTAYDLCETDPLLLNEMGVVYFHQDQFPDAITFFRRALAYAERNGADTDAWLPTRINLAHALRKYGHLDEALATFEQVLRHGVKDPAVFTAKGLVLLELNRSWDAVIALHEALAVSPQDPMATDLLNRALEANEVDSGLIGDTGPGAEAGGFGGEDGSGGGGAAAGAIGGGEEEIEELERRMNGRLREIEQNRVAGRRGGRGRRRVPSSGVELPLVDDASAFGESMVVDSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSH2</accession>
    <name>A0A9W9CSH2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000809</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSH2"/>
    <dbReference type="OrthoDB" id="5840532at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42820">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42820:SF1">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="287" mass="30474" checksum="F8AFEA1D61897718" modified="2023-11-08" version="1">MSKRSGPLAPGVAFITGGARGLGNAIAVSFAKEGARGVVLVDVQDEKTFKEGKQKVESYGTECLTIRADVTKEAEIEYAVQQAVQKFGRIDYAANFAGILGPSDPISETSLADWQKCQDVNSTGILLAVKHEMRQMIKQSSIDGIEDGRVGQQGSIVNCASVNSLLSLPGSVAYTASKHACHGITKAAALEGRAHNIRVNAVSPGFLLTKLVQPVVEGTFELEEGGDGKKNDAGKAMWAEFEARQGRSARFEEIGDVVVLMSLPRMSLVNGQNLFADGGFTINEGNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXR7</accession>
    <name>A0A9W8XXR7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010782</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXR7"/>
    <dbReference type="OrthoDB" id="2119228at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01833">
        <property type="entry name" value="XynB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051532">
        <property type="entry name" value="Ester_Hydrolysis_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30383:SF2">
        <property type="entry name" value="CELLULOSE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30383">
        <property type="entry name" value="THIOESTERASE 1/PROTEASE 1/LYSOPHOSPHOLIPASE L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040817806" description="SGNH hydrolase-type esterase domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="SGNH hydrolase-type esterase" evidence="2">
        <location>
            <begin position="30"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4361841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="244" mass="26620" checksum="2B685DE4C9139389" modified="2023-11-08" version="1">MVSFKLLSLAALAATASAQTANTTLRYMPFGDSITEIVCWRAKLWEKLQSTEWATVNFVGSGKTENNCKDTKYDRDNEGHSGFLAIDIATKDQLTGWLKANPADVITMHLGTNDIVQQNKNVVDIIAAFTKLVGTMRTSNPKMKIVVAQIIPLGLGNYNSKIQDLNKAIIPWAQGLNSTASPIWVVDQYTGFSGTSDLRDGVHPNDAGDVKMANVWYPAVVHAFEEANKDKVAAEGIKEISFVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXW9</accession>
    <name>A0A9W8XXW9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytoplasmic tRNA 2-thiolation protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">CTU2</name>
        <name evidence="3" type="synonym">NCS2</name>
        <name evidence="4" type="ORF">N0V83_010471</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CTU2/NCS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXW9"/>
    <dbReference type="OrthoDB" id="25129at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03054">
        <property type="entry name" value="CTU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019407">
        <property type="entry name" value="CTU2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20882">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20882:SF14">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10288">
        <property type="entry name" value="CTU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4362378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="310" mass="33889" checksum="C65F19EC200BCF68" modified="2023-11-08" version="1">MPGKHLADLHLKTQKSKTGRTGFAIHAVLIQDPEQSIPTDELLEKVRQNYPDHHYSSLPLHDVFRLIPDEASLRALVPEVATKEDLTPQDHLAHLVNSLTSATARADVLTTLRTRLIVEHAKLSGCESILWGDSTTRLAEKTLAETAKGRGFSLPWQIADGQTPFGINFHYPLRDVLKKELVSYINMAEPSLATLVYEPSSGATQASMSSKNTTIDDLMKQYFESVEENFPSIVSNVVRTTGKLDRPATSTSDTYCSLCSMPVPGGRFGIHGWGGDQQDGVNAESNEGTSNLCYGCTRSIPQVPAKVNGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY33</accession>
    <name>A0A9W8XY33_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RPC11</name>
        <name evidence="14" type="ORF">N0V83_010882</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 12">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY33"/>
    <dbReference type="OrthoDB" id="282152at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10509">
        <property type="entry name" value="Zn-ribbon_RPC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034014">
        <property type="entry name" value="Zn_ribbon_RPC11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF12">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="13">
        <location>
            <begin position="75"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="10">
        <location>
            <begin position="5"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4361941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="118" mass="14027" checksum="92C6B64B4C752777" modified="2023-11-08" version="1">MLLFCPTCSNMLRVSKVPPGDPTTEEYVGQNRFECLTCPYHFVINKRYYERKYMKKKEVEDILGGKGAWDNVDKTQVQCPNEKCRNDEAYWYQLQIRSADEPMTAFYKCTKCAKEWRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ14</accession>
    <name>A0A9W8XZ14_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_010012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ14"/>
    <dbReference type="OrthoDB" id="3724345at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="entry name" value="dinb family like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018531">
        <property type="entry name" value="DUF1993"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36922">
        <property type="entry name" value="BLL2446 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36922:SF1">
        <property type="entry name" value="DUF1993 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09351">
        <property type="entry name" value="DUF1993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="DinB/YfiT-like putative metalloenzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4362895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="195" mass="21323" checksum="550D9A652389A2A3" modified="2023-11-08" version="1">MADPVTYTFYGTTVPVLRNIATSAISILTTAKDELARAEATGTFPSEAELLSTHFGTMLPLRMQPILLAKFQVAALQNLQLNGSAPIPALDPASFSSFDDVIDFFRKLQAVFDAVDEKTFNESAEKSVEIGVGAKTLHMAGLADYFHGFVVPNSYFHLNAMYMLLRSKGFALGKGAYIGCWMSEQQKKDWAPLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZH4</accession>
    <name>A0A9W8XZH4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">40S ribosomal protein S11-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RPS11B</name>
        <name evidence="6" type="ORF">N0V83_010255</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZH4"/>
    <dbReference type="OrthoDB" id="10254436at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.1000:FF:000001">
        <property type="entry name" value="40S ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028333">
        <property type="entry name" value="Ribosomal_uS17_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019979">
        <property type="entry name" value="Ribosomal_uS17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032440">
        <property type="entry name" value="Ribosomal_uS17_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03630">
        <property type="entry name" value="uS17_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF9">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16205">
        <property type="entry name" value="Ribosomal_S17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00056">
        <property type="entry name" value="RIBOSOMAL_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS17 N-terminal" evidence="5">
        <location>
            <begin position="8"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4363135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="159" mass="18427" checksum="25CBF78A7072156C" modified="2023-11-08" version="1">MATELTVQSERAYQKQPHIFLNHKVGGPRKNTRNRRWYKDVGLGFRTPKNAIEGTYIDKKCPFTGMVSIRGRILTGTVMSTKMHRTLIIRREYLHFIPKYSRYEKRHKNLAAHVSPAFRVEPGDQVVVGQCRPLSKTVRFNVLRVLPRSGKAAKQFSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZM9</accession>
    <name>A0A9W8XZM9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Malic enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">MAE1</name>
        <name evidence="15" type="ORF">N0V83_009437</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(S)-malate + NAD(+) = pyruvate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:12653"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.38"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>oxaloacetate + H(+) = pyruvate + CO2</text>
            <dbReference type="Rhea" id="RHEA:15641"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="EC" id="1.1.1.38"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10">Divalent metal cations. Prefers magnesium or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the malic enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZM9"/>
    <dbReference type="OrthoDB" id="5365701at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004471">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05312">
        <property type="entry name" value="NAD_bind_1_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10380:FF:000001">
        <property type="entry name" value="NAD-dependent malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000055">
        <property type="entry name" value="NAD-dependent malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015884">
        <property type="entry name" value="Malic_enzyme_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010052">
        <property type="entry name" value="PRK13529.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406">
        <property type="entry name" value="MALIC ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406:SF34">
        <property type="entry name" value="NAD-DEPENDENT MALIC ENZYME, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00331">
        <property type="entry name" value="MALIC_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4 11 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="14">
        <location>
            <begin position="93"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="13">
        <location>
            <begin position="284"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="584"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4363983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="604" mass="66970" checksum="E5CBD02B8AD8CB9C" modified="2023-11-08" version="1">MSATKDQNTNKFGKLPLSTSGPQETSLTGNALLRTPYFNKGSAFTKEERDTFKLHGLLPTNVQTLDEQVKRAYTQYSSRPNDLAKNTFMTSLKEQNEVLYYKLILDHLKEMFSVIYTPTEGDAIADYSRLFRKPEGCFLNIDDADRIEDDINQWGEPDDIDLIVVSDGQQILGIGDQGVGAILISVAKLVIYTLCAGIHPSRTLPVVLDCGTDNKELLHDDLYLGLRKDRVCGERYDNFVDKFVTSCRKRYPKAYIHFEDFGLNNARRILDKYTPKIACFNDDVQGTGCVTLAAIMAAFKVSGVKWDEARFVMFGSGSAGTGIADQIKDAISQNTGKSREDAGRQIWCVDKPGLLLQSKKDQLTPAQLPYARADKEWESKAHGDLYSVVKEVKPHVLIGTSTKPGAFTQEVIQEMAKHVERPVIFPLSNPTRLHEAKPQDLYDWTDGKALVATGSPFPPVKHNGKEYDISECNNSVTFPGIGLGAVLSRTRLMSPALLVAAVQALASAAPVLTNSGAGLLPDVEDVREISVQIAKNVIKKAIEDDLAQETDIPTNDEDLEEWIREQMWNAEYRPLKLVEEHTADAHAKGEAGTASGRREARFDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZQ5</accession>
    <name>A0A9W8XZQ5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Structure-specific endonuclease subunit SLX4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">SLX4</name>
        <name evidence="11" type="ORF">N0V83_009483</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Regulatory subunit of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a heterodimer with SLX1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="9">Phosphorylated in response to DNA damage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SLX4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZQ5"/>
    <dbReference type="OrthoDB" id="5349119at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033557">
        <property type="term" value="C:Slx1-Slx4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03110">
        <property type="entry name" value="Endonuc_su_Slx4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027784">
        <property type="entry name" value="Slx4_ascomycetes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018574">
        <property type="entry name" value="Structure-sp_endonuc_su_Slx4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09494">
        <property type="entry name" value="Slx4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="59"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="128"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="361"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="419"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="689"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="792"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="842"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="162"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="174"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="239"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="251"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="487"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="865"/>
            <end position="880"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4364028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1023" mass="111255" checksum="4EBF9C4A50AB5F25" modified="2023-11-08" version="1">MGIDVGPVPMPFPATEQPPYDGQAATADSAQLAAWRRLPRLSMAGTCYEIVVLSSSPPAAELYDAPSPPKGFSPATRRVAMSPPSPLAFSPPASPERTAAGASKTNPRAAPIPEGVVRGFATVGSLVRSEHFADQPDDDLTKTQQPQSRRRSLEGAEEKSRVSNKPRKRAAKKSTADDTTEAKPKPRARKPKADKPNTALDPDLRLPASTKSPFFPIEDAVPPIDAPKETADPAPKLTKSGKPRKPRAKKQPPEHGKAETESKPKRTRTTKPKGAAGAGKLQREGASVVSSHFRGNGDRGEEAAADELGPRKSADLQNAADESISLWEIPPSPRPANKAPVKQRPPDPIAEGLQLEEAVARRRDWTPPPQDTAVTTPFTDSVGKENKQSAQDANGTFTHMLSNFAYARSPSAQITLKTVSSTTDATAATKRRRVELIEVPSNQSNSRNSSPEKGKAPKKKPRTITDLVTGQYAPKDPSPDPHAVMSNFFQPPTTTTKVPLNDVSAPEADASLKKPPLRRSKSKSNSEDTGPKAKSKKTSGKVAKPKPVAEKLLSPTSAVLRMNRQDVLFGTSSQLALEESPGMVRQLQLALKESELDIATSPELHLPAPPRWPRLLKVEGKRKLWAAGARDDDGSMLEHMEDVYIPEPDRTQDISLLMDGTHDESDSVPAFGHIDDLDPPPLIAISSDLPTPPRTTSQASQHIKPDDVASPFRELVFEDIDDFEQEPPPSNQNVDSQNSFADIEDFHFPPSAQLLKSAQPTLRPPISEHADEIPMQRPGTLPKPRATIPAVAESSTMKLNPGPSQPNRKPKMPHIPLTPPKSSSRFIDIEEILDSEDEALEALSPTPPRTRNIQDCEPLPLVSLDSSPTKATSKPTSDTSALTPVFRIPTSSLQWEHIKADIFSRITLHIRTLPPTTDPKRPCWHEKILMYDPIVLEDLTAHLNSTTTIRTYKKATQKQIKAWNQKLKSDAQAVLSVGENGEEVLAIEKALETYMVQGWCESMSVCCIYGEGRGHGGARKGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZV5</accession>
    <name>A0A9W8XZV5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_009616</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZV5"/>
    <dbReference type="OrthoDB" id="5022096at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF93">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040966739" description="Rhodopsin domain-containing protein" evidence="8">
        <location>
            <begin position="27"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="9">
        <location>
            <begin position="11"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="247"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="324"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="347"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4364161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="356" mass="39781" checksum="A89BE7A33C7D42C3" modified="2023-11-08" version="1">MEINIPQVLVLVNSILLLSTVPYAFGMPPTSFYLSDAQSAFRHAAIAQPIWAWAMAAVKTSFALMLLRIEQSVRLRRFLWAMIVIQVILGVYNTLTILLQCVPFYKAWDLVGSVPGTCWARRAQSISTICVSVLNILTDFALALMPISFLRKIQRPVRERISVGALMGLGVFAGIASILKIVAAAQFGRTGDSVNESVMIGMWSVLEELVGLIVICIPCLRSPFHRALEYCGVLSVRIRQHTLTRGYGRTYDRPDEAKKSNRSQSRSQSQSCLATTIGDSNPDAGFKLDNLRTEGSQEHILYEPPTRPGEIWCTKEVMVENDRISRMPSYERPVGGPDASWSDEPFNLDRRDLERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y061</accession>
    <name>A0A9W8Y061_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_010049</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y061"/>
    <dbReference type="OrthoDB" id="655030at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051704">
        <property type="entry name" value="FAD_aromatic-hydroxylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46865:SF2">
        <property type="entry name" value="MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46865">
        <property type="entry name" value="OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="4">
        <location>
            <begin position="9"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4362932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="428" mass="47616" checksum="39C073F236DEBF6D" modified="2023-11-08" version="1">MSASTPSLNILISGSGIAGPALAFWLHKFLPSSQITIVERSPEPRLGGQAIDLRSSCVPIVDRMGLLGKVREKTTTEAGMEFVYADGVRKATFPVSGNMEQQSMTSEFEILRGDMAKILYDESKELENVKYVFDDMITAVEEKDSGKVEVTFKNNLPKAEYDLVVGADGMMSRTRRLVFGHGPKNDDYLYRLGQYSALFTMPRTDEDTKFAQWYNAPKGRLFLLRPDQYGTTRAYISVTDSNLSRFDKIDKLLKEGTREQQEAWFEEEFQGAGWQTERVLTEMKNADDYYVQQIAQVKMDTWVKGHVVLLGDAAYCPSPISGVGTSSAIVGAYVLAGELSKSPTDIPHALSQYDKVTRTYVDKAQRLVPGAPQLANPQTEWGITFFNTVTGVFAHPFAKRFGSVVGSWFLAFGGSTVWSPPDYGRAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y085</accession>
    <name>A0A9W8Y085_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nucleoside transporter family</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_009916</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y085"/>
    <dbReference type="OrthoDB" id="46396at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034257">
        <property type="term" value="F:nicotinamide riboside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002259">
        <property type="entry name" value="Eqnu_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332:SF88">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01733">
        <property type="entry name" value="Nucleoside_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016379">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="285"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="390"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4363620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="454" mass="49603" checksum="724AD6DA0C5CF82B" modified="2023-11-08" version="1">MDRVREIFERENSRASYERLVGGSEDIDGIPIEQSEEQPFSWVDYSIFVLLGVAMLWAWNMFLAAAPYFQHRFASNDNLLRNFQSAILSVSTVGNLGSVIVLSELQARANYPKRIKIALSLNAAVFTLLALSTKLFLNVSAGVYFAFLLIMVLSASLATGMCQNGIFAYVSGFGREEYTQGIMSGQGIAGVLPAITQIISVLSVPQKKQHIDDGQPQASSASAFIYFLTATLVSGITLLAFFHLLSRPSSKDRMRHYDSHDTLEPSGAHSGAHKRIPLLRLYSKLFWLATAVFLTFAITMVFPVFTAQITSVRDPATAPRLFQPATFIPLGFFFWNLGDFLGRTGPALPSLRLTHRPRLILLLAIARLVFIPMYLLCNIGGNGAVVQSDFFYLFIVQFGFGVTSGYLGSNCMMGFVEYVESEEVEAAGGFMSLALVGGLTAGSFLSFAAAKAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y093</accession>
    <name>A0A9W8Y093_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_009100</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y093"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000090">
        <property type="entry name" value="MFS sugar transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF45">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="270"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="408"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="13"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4364505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="512" mass="56197" checksum="663FA7ADF4C9BEC7" modified="2023-11-08" version="1">MGLTGKTLQTAQLLLVVLPAFVLFGYNQSGVGGLLSLDDWNEHFPQIDTVDATGAEKSHRSTVQGAVVATFTLGALCGALSCSWIGDIFGRRKVIFGAAVLTLIGEILQCTSFQLAQFVVGRFILGWGVGALSATVPVWQSECSSSANRGKHVVLDGCFISLGYLLEAWINLGFFEQDNKPLQWRLPLAIPTVISLVPMLAVFSIPESPRWLVNKGRVEEARSSLSAFKGLPSTDPSVGAEISGIELALEETGHSAAKIRDIFTMGKDKLFYRFSLCIFLQFLQQMCGSNLISTYSTIIFQQGLGLDSETSRILSGGALTWKFLSCFVAFFTIDRFGRRKLFMFSGVGMASCMLALAVASSFPKTNQPAQIASALFVFLFNFFIPIGFLGANFLYCTEVAPTRLRVPMAGISTANHWLWNFVVNMVTPVAIETIGWQYYLVFLIISAIIVPIVYLFYPETMGRSLEELEMMFSEGRSIRGIVRESRKPLDGSLLPEHALEKGGHADEDEYAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0B8</accession>
    <name>A0A9W8Y0B8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tap42 interacting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TIP41</name>
        <name evidence="3" type="ORF">N0V83_010155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIP41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0B8"/>
    <dbReference type="OrthoDB" id="10253878at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051330">
        <property type="entry name" value="Phosphatase_reg/MetRdx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007303">
        <property type="entry name" value="TIP41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021">
        <property type="entry name" value="GAF/PUTATIVE CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021:SF16">
        <property type="entry name" value="TIP41-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04176">
        <property type="entry name" value="TIP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="269" mass="30866" checksum="435A32F46C9E99F5" modified="2023-11-08" version="1">MSALNGAPVDTQAPGHADTSITKGDFKITTRKLPILKAGPIEDMTKKLGIAPPEMIFGDNLVRIEHAHSGWHIEFNAFDALDLVDKTGEKMFKVSYSKEWQQNRQKQFEDIKEVVKPFDWSYSTDYKGSTPPTPAFQPTDSPIPLALLKRPDPIHFFDEVMLYEDELADNGIAMLSCKIRVMPARLLLLVRFFMRLDDVVFRIRDTRVFVEFGTDEVIREYTAREEKYEEVRKKLAGRKEDVLAIMRDPNRLAEHIPVVEKSLEGLKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0Y8</accession>
    <name>A0A9W8Y0Y8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">mRNA-capping enzyme subunit alpha</fullName>
            <ecNumber evidence="3 16">2.7.7.50</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 16">GTP--RNA guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 16">mRNA guanylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">CEG1</name>
        <name evidence="21" type="ORF">N0V83_009855</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Second step of mRNA capping. Transfer of the GMP moiety of GTP to the 5'-end of RNA via an enzyme-GMP covalent reaction intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 5'-end diphospho-ribonucleoside in mRNA + GTP + H(+) = a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:67012"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="Rhea" id="RHEA-COMP:17166"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167617"/>
            <dbReference type="EC" id="2.7.7.50"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:67013"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Heterodimer. The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an mRNA 5'-triphosphate monophosphatase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the eukaryotic GTase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.50" evidence="3 16"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0Y8"/>
    <dbReference type="OrthoDB" id="200924at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031533">
        <property type="term" value="C:mRNA capping enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004484">
        <property type="term" value="F:mRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07895">
        <property type="entry name" value="Adenylation_mRNA_capping"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001339">
        <property type="entry name" value="mRNA_cap_enzyme_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017075">
        <property type="entry name" value="mRNA_cap_enzyme_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013846">
        <property type="entry name" value="mRNA_cap_enzyme_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051029">
        <property type="entry name" value="mRNA_Capping_Enz/RNA_Phosphat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367:SF17">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03919">
        <property type="entry name" value="mRNA_cap_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01331">
        <property type="entry name" value="mRNA_cap_enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036959">
        <property type="entry name" value="mRNA_cap_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9 16" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="5 16" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 16 21" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16 21" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="mRNA capping enzyme adenylation" evidence="19">
        <location>
            <begin position="46"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="mRNA capping enzyme C-terminal" evidence="20">
        <location>
            <begin position="254"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="390"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="active site" description="N6-GMP-lysine intermediate" evidence="17">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR036959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR036959-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF03919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ4363559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="424" mass="48675" checksum="4F89876CFF95B5C9" modified="2023-11-08" version="1">MPATVISHAPPPQMPGFLIPGHDAQALKEAVANLLERDNLRFPGAQPVSFAREHVAELQRQEYFMCEKTDGLRCLLFLHWQDLGGPDVEPATFLIDRKNNYYNVTPPFNIPHYMHPNDPDKFLFGTVLDGELVHDQYPGEPEPRLNYYVFDTLAVSNQNVTSKTLDKRLGRLQEWVLKPYHASLKKIPNIKPDDLKPFALKDKKTYPAYSLQEMFSNVLPSLKHGNDGLIFTCKSTRYEFGTDRHILKWKPPHENTIDFKLRLGEFPLIDPGDGGDSIPDYDAMPVPFQLLVQHNQNNYQPFATLSVRPDEWDMLKGLNQRLDGRIIECYRHPNGQWMFKKEGDGTPRWRDDKKDANHISTVNSVLASIENPVTELDLLENQENIKKEDYARKGKEYYPPQDGSGERDAKKRKFSQAELNGGPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1L9</accession>
    <name>A0A9W8Y1L9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_008717</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1L9"/>
    <dbReference type="OrthoDB" id="5344325at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF84">
        <property type="entry name" value="FUNGAL SPECIFIC TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="4">
        <location>
            <begin position="172"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="458"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4365099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="562" mass="64142" checksum="9E9FC4D73693FACA" modified="2023-11-08" version="1">MEDIGQRNKEPPLDVPASNAVDKILHAIPPRSITGTKDRGSFRINNNLRADTFVNHFLTVVNYRYNAIYRPTLTDQYVQWWADRAAGKPLSPEFTCLLLRICAYSVQYLSPALRKMIEFELACNSQTLTERFTSAADQLSQSFEASTTRIERVQEQFLKAVWLKSESKIVESWHTLGRTIREAQELGIDKDAGIDSLPEFDIEIRRRIWTLLYIWDWQMSAWLGRPNLIDQKNLTFNFPNLRLDQSTTEPKLLSPFAHIVLQAQLARRIATIMGEAQPHTNLSAEQVLAIESECEKFMEELPAVFKITETDKSLDEQYPYFVFQRHQLHCVIFLAMLDVLKPYLTRDRRDKLTDRDDELRKMGVDIALRLLEVSRDLFDHEFPINAKFHMVAFSIFDTATLLCSAIIHDQDHILPRREAVIDIINNSLDMLHQLSLTTKLGAASYSFLSKLVQATPELSRHSPGSKRQKQRDLLTPSTSSSSLAISTMDHMEPLTGTKPPEINLVPSVTITDDLSFDLDQFLAQNPFGNPGSTSALDMGGMEQIWDWENLHLDGFSNNGSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y256</accession>
    <name>A0A9W8Y256_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008814</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y256"/>
    <dbReference type="OrthoDB" id="3743447at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="222"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="11"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="290" mass="32308" checksum="1CF93A35A99D8D6E" modified="2023-11-08" version="1">MRQDPVDHESPSSSEMSRKPSIASTSSSSLSLYQSSRSSSPTTGYLEPSYDHSPPVNRVAMNTGHFRPYPTPHLPIVSSLRCPSCSEAGETIFVEPGKACPRCGQLDPKSKATGKVARRKRSDRIHFSRLMNNDGKPEKASKEEGEAGRRYDHSFYFASLQNLLLKINPDFPEVAAQRGGKKRPGWTPLKCNNISNDVKEGSNIEPLHYNKTNMFESSLQIIEDSTAILSDAIQERNDLEVEMDALLRSEAPAEELRRFLMRTLHRNWSNTLATSSAQRGAEGFSRPSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2E8</accession>
    <name>A0A9W8Y2E8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aldolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008936</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2E8"/>
    <dbReference type="OrthoDB" id="197068at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00502">
        <property type="entry name" value="DHQase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000135">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001381">
        <property type="entry name" value="DHquinase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050068">
        <property type="entry name" value="MurA_subfamily"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01093">
        <property type="entry name" value="aroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43783">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE 1-CARBOXYVINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43783:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE 1-CARBOXYVINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01487">
        <property type="entry name" value="DHquinase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4365316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="680" mass="74407" checksum="0ECEF4F0B2D7B74B" modified="2023-11-08" version="1">MATMVQAGSVTTSQLKDGTVIDVQDIPSGERPALLILHNEGEEDIVGIFADVLGQKWESTATVDEAKAGPPSTVYGLSSAIVGTQLDNWDRSRLLINTHRVDGGQELNEALTDRCDYEYLYTQSPFFRRDVSRFLSFILGQIAPHRDLTKKARTTLISTTFPDVHAALPNLDILSVGADAIEIRVDLLEEPLPDGSVSKIPSLKYVGEQLMVLRQRTELPIIYTTRCTKENGRFPMEDPSLFYKYLARAIQWGCEYIDVELWLPEEIRRKLAENKGCSKIISAFHDFSGTFKWTSPETQTLFERGAVYGDIVKMYVLINSMQANYELEYFRSTIQAKYPHPPFSGLNMGPMGQLSRTMNKIFTPITHPLLPMIAAPGQLSAAEINAALHTMGQMPKQDIYGIGSFRSTSQAMFFEKCFNELSLPHSFGFAERAPKASIEHILRRPNFGGAYINPPLAASASYLPSLSNAARAIGQVDTVLVRPGTSSASSFIGDNARWKGIRSTLTRDFVPSAYSGQAALLLANSEADASASIFALKNLGIGPIYTIGFRSHGPLSTDVSPVRSVEDVKRLEQPFVIISALPAEKSLLVVPLLKHYRVDGRNGNTNGHGAAAGKPAGKVFVDLASGPRKADPLAIATSAGWTAYGIADVSAWTTVETIRQLVGQSVCYDFVRLASGRGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2R2</accession>
    <name>A0A9W8Y2R2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">GCN5</name>
        <name evidence="16" type="ORF">N0V83_007824</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetyltransferase family. GCN5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2R2"/>
    <dbReference type="OrthoDB" id="1937912at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000123">
        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010484">
        <property type="term" value="F:histone H3 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05509">
        <property type="entry name" value="Bromo_gcn5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000004">
        <property type="entry name" value="Histone acetyltransferase KAT2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037800">
        <property type="entry name" value="GCN5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750">
        <property type="entry name" value="GH11602P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750:SF3">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="11 16" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7 12" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="15">
        <location>
            <begin position="118"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="14">
        <location>
            <begin position="354"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="14"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="47"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4366189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="454" mass="51269" checksum="45BA95767EEC8B37" modified="2023-11-08" version="1">MALPKGVPLLRDMLGYATSPSTSPFPSPPSGGASAAFTSPEAPLRAQHADRDADLAKRKATSEAANGSPKRIKQSTPSTPPAADNGPAPPVKRIVPFPEKPAVIEEREGEIEFRVVNNDGRRESTIILTGLKCIFQKQLPKMPKDYIARLVYDRTHLSIAIVKKPLEVVGGITYRPFDKGQFAEIVFCAISSDQQVKGYGAHLMSHLKDYVKATSEVMHFLTYADNYAIGYFKKQGFTKEITLEKSRWMGYIKDYEGGTIMQCSMVPKIRYLESGRMLLKQKECANAKIRAVSKSYEIHQPPSQWSKGDLKPIDPLTISAIKNSGWSPVMDELARAPRHGPNYNALLHLLNDMQNNSNAWPFQQPVNKDEVLDYYDVIKEPMDLATMEEKHEKDLYPTPEDFIRDSKLIFDNCRKYNNESTPYAKAATRLERYMWQRIRDIPEWSHLEGELTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4K5</accession>
    <name>A0A9W8Y4K5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pre-rRNA processing and 40S ribosomal subunit assembly</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">FAF1</name>
        <name evidence="2" type="ORF">N0V83_007353</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4K5"/>
    <dbReference type="OrthoDB" id="5556956at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027973">
        <property type="entry name" value="FSAF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053030">
        <property type="entry name" value="Ribosomal_biogenesis_FAF1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28096">
        <property type="entry name" value="PROTEIN FAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28096:SF1">
        <property type="entry name" value="PROTEIN FAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15375">
        <property type="entry name" value="FSAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="170"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="312" mass="34005" checksum="344AF5D3568F5672" modified="2023-11-08" version="1">MAPTLGKRKRVSRAELEQASRSLSPSSHSNDSGAEDLQAIFRRAFEAKFKPLPVETKKPKIEEAQIEDENEDEEEEPDWSGISDDESTNQVEVIEFKDTRHARDETEKAEKKAFMTSKPPTSTPSSLAPTKSSSLTVKKSQEDANDPTDKTNLKNDLALQKLLRDSHILSTSSSGTSTPTTSLSAIGSQRHKSTDLHLLTLGAKSSVFTQKNMPMAQRKHEAAKKRGMEEKRRARAKEAGIVLEVQRSSSSEKKGKGKGMGRGEGERGWWAECGEVQGGTLSLSREDVRSITGGRGGKGGKGGKGKGRTGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4M0</accession>
    <name>A0A9W8Y4M0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_007987</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays an important role in cellulose degradation. Shows hydrolytic activity against several glycosidic compounds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4M0"/>
    <dbReference type="OrthoDB" id="65569at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080079">
        <property type="term" value="F:cellobiose glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000011">
        <property type="entry name" value="Cytosolic beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF36">
        <property type="entry name" value="LP05116P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4366351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="480" mass="54937" checksum="4002FE45FB2757A4" modified="2023-11-08" version="1">MSQSYLPKDFLWGFATASYQIEGAPHEDGRADSIWDTFCRIPGKIAGGESGEVACDSYHRTAEDIALLKQTGAKSYRLSLSWSRIIPLGGRNDPVNEKGLQHYVKFVDDLRAAGIEPMITLFHWDLPDNLHKRYEGMLNKNEFVKDFENYARVCYKAFGSKVKYWITFNEPWCSSILGYCTGLFAPGRCSDRNKSAEGDSSREPWIVGHSLLIAHGAAVKAYREDFKPKDGGQIGITLNGDWTEPWDPEDPRDREACVRKLEFAICWFGDPVYFGKYPESMRKQLGDRLPQFSPEEAALVKGSNDFYGMNHYCANYIRHKDGEPELDDYAGHLEILYQNKKGEWIGPETQSVWLRPHPLGFRKLIKWLSDRYGGPTFYVTENGTSLKGENELPLEQLLDDEFRCEYFRGYIGALADAHTIDGVDVRGYSAWSLMDNFEWAEGYTTRFGVTFVDYEGGQKRHPKKSAREMSKIFEKHIKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4Z5</accession>
    <name>A0A9W8Y4Z5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007317</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4Z5"/>
    <dbReference type="OrthoDB" id="4476201at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF82">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26639">
        <property type="entry name" value="Het-6_barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="171"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="932" mass="106015" checksum="3A6C1910F2AB56AF" modified="2023-11-08" version="1">METYTTILDREASLEPSPHDANALPTSFYEALPTATSVRLLDVLDSQRDGIRCRLRTVDLEDETGFAALSYTWGNPTTVYDEPLPDTSHLRFPEDANQFPFMYTTGPLGPEGETMASVDCIKRDYVSIYPRIPYEKVAWKTGTTRQITVNDCVLEVEENLFEYLTCIISMRSRYPHKKGNGLDHLHDYSRLPMWIDALCINQNDLSERAAQVQLMGRIYKSAKIILAWIGQDDELCNLAFTSIDKILAWESAHMDDIFGKQDGNTPRRRDQDHVTLSSIPDMTVVHWFALFALFQRLWFRRAWIAQEAIFAKDLLVICGGNLIGFKLLLLVFAILEDNGLGYELCRVGRNLLTGQPVAASTLQLENFGALPRESEQTISTAHSHQLSVVPWDVYSFVLGYHRVRGCLGLSEVGRPMLWPWKPEEIKRAEHSLYNVEIPTEVKLKDTIVEELEPGVFRFHMRALRLLSILSDFRNLNATDPRDKIFAFLSLATDNLGLIPNYHTSVQEVFLDATKQMMIQYDSLAVLSHCQDPANTRILGLPGWVPDFSVRLGRSLFDTGGEISEFSASGHYPPHLDFCLDGSLEVEGCRVDTVSTTTDLEGDAVVKILTLLLQIPAKYSVIPLVSCHKSLPDNKSRRELQPRIMSRIEALWRTLIADALTEVDELPDSLDSRDALSSGFRIWILIDILEAHGILAQFTDVDRGHWAAQLIYDSFRTRMALWSAIYDNQHIPESSNIELPSVEEVIMDFATKQEDEKDQYQLDQHLGLEHPTIGLEHFPTATELVECFNKYNDESDQEEKAQRSSLNGSYRPDTLQRLTNLQRRQLRNFEKHMRKATEGRRLLVSARGLLGLGPMSTAQAETRKDEIWILRGARVPFILRQMGEGQYQIIGEAYVHGLMYGEAVRSRDAETFWHDLHGEMSKSLPGYQKIRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5H8</accession>
    <name>A0A9W8Y5H8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_006541</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5H8"/>
    <dbReference type="OrthoDB" id="361013at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005679">
        <property type="entry name" value="Ribosomal_uS12_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01034">
        <property type="entry name" value="RIBOSOMALS12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="137"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="137"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="166"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="219"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4368185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="490" mass="49352" checksum="D27A62A3B908E5E0" modified="2023-11-08" version="1">MPLTFLRRLVRPSIISKAPFVTPLPLRSSIAATAFQNATSFAPNQSIPSSRAHKCTLIQVLRQGRIARKARKLRSPQLKDRPELKGVCLKVGTTKPKKPNSGERKIARVRLSTGKVITAYIPGEGHNVQQHSVVMAVTSSQATSSSDPSESQVVAPPGPPGPPQPQATSSSDSSQSQVAAPPGPPGPPQPQAQYQAADATTADSSQTEAAAPPGPPQPGQAVQPVQIQAVQPQAASTPAFHIEEEPAEAAAPVQPEAAAPIAPAPVQPQPYQAATIAPKPFHTEAEPQEFHAEEAPAEPEAVIPCQAGILHHLPLPVPIPQQVTEPVSGVLSNVPAPVACVPQGLPVSVASGVLEDLPVPAALPAALPAALPAALPAALPAALPAALPAAAPLDAIKPITGVLSNTPAALPVAGVLAAPAAAAPLSAIKPLTGALSIAALPAALPAAAPLQAVKPITGVLSNAPAALPVAAPLQVAKPLTGVLGNVPVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y671</accession>
    <name>A0A9W8Y671_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006554</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y671"/>
    <dbReference type="OrthoDB" id="2506647at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4368198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="168" mass="18528" checksum="900439277051C350" modified="2023-11-08" version="1">MFLDLMMNDDTYFGQVRHWLVTNVSTNSDGSLCTPQDGVISPYIGPAPLPNYIYPRPHRYIFILARGTGKVDITSEDLRELQKPYTAAVAGNQGAVQDLKDRWGFNAMKLIEKKGLKVEAVSFMRVGGTLKSAAANMAMMGQAVVNKVSAVACSGVWRFLLILKISRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6G4</accession>
    <name>A0A9W8Y6G4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetraspanin Tsp3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006313</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6G4"/>
    <dbReference type="OrthoDB" id="71600at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="321" mass="35381" checksum="6BEBB7A623786B8A" modified="2023-11-08" version="1">MASGVELPPLQGARISLIGDNTSSSPSALQGKSPRNLASLAADPRRYASSRANARSVPISDTLTGFTTALPVVAGLLLECGYDLTRRQERRQQLGRGEIQRPPLVIIANTLIFIYSSVVITLLGTHAAPPSGLDCGLRGRWQTLFKQKDADAIRTIQDAFKCCGFANSRDMAWPFPDRTHDQHACESAFGRTNGCLGAWRAEEQNIAGLLIGVVGMVFIWQFAIIAIPTQRESWLHRVAPDRISRMIADERHGGSDEPRRAIDYIPGYNHYSDRVEEEAENDDMNSGRAIEEGNRRINNVVLPGHLAPDQQPTVENEWARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6H4</accession>
    <name>A0A9W8Y6H4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_006686</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6H4"/>
    <dbReference type="OrthoDB" id="5022096at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF96">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="49"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="318"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="318"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="361"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4368330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="429" mass="48177" checksum="FFEE7F0355C79DCE" modified="2023-11-08" version="1">MVYRLIASRQAGGSPPPQRSPEYLAENQAPRLLAVEGTLFALSMSAVLLRFYVRIFMLKTFGWDDWMMAISAHQQEHKQLTEIAQCLSATCLGLFVTVLNMGLGLHAEAFPPENIFPFFKYMYFYSILIIAAYSFIKLSIGFFLLRLADRTKWRPFLISMLVFIACFTIGSTFAIIFQCVPVRAGWDFALRPPTGTAKCYDATIFKNVGVFNSSINIATDLIFALIPIPMVWRLQVNMKTRIGLAVILSLGLFASAVAIYKTPMQYNFFKETDWSGKGSWYYIWQQVEMHVGILAACLPTLKPLFSNFFGSIRTFTKGRTSRTTGSGISTPFKSNGYMKHPDDQGQGSFAMKNLSEGSQSRSRDPYEQDVVMGKDKSYTVEAGRGRLHRPRSQAGESDESILSHDAPRTRSSLARGMTIIKTTEVNVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6V3</accession>
    <name>A0A9W8Y6V3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">N0V83_005481</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the RBR family. RNF14 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6V3"/>
    <dbReference type="OrthoDB" id="1431934at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20354">
        <property type="entry name" value="Rcat_RBR_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23134">
        <property type="entry name" value="RING-HC_ITT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23820">
        <property type="entry name" value="RWD_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000061">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000112">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000416">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047548">
        <property type="entry name" value="Rcat_RBR_RNF14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RWD" evidence="14">
        <location>
            <begin position="11"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="161"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="165"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="281"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="286"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4369718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="474" mass="53763" checksum="93CC1C69D297EC7E" modified="2023-11-08" version="1">MADTDDDERTEELETLQSIYPELVLDPNQPYTATLDLLVAPTKPLPVTFEPENDVARLAYLPPLHLEFILPADYPATSSAFVKPSTSPSWLPEHVLKRLEDEAEAIWEEYGGGTILFSYISSLQEQAEAAFGLEDLSLPSSMRKELVDYSRRMKKELFDKETFDCEVCLEPKKGSACYRMERCSHVFCIACLQDYYNSCIKEGDVNNVKCMSTDCGKSGNKAKKDRLLSPKELLQIPISREQVERYAKIKRKKKIESDPTIIFCPRSWCQGAMRTDRHPKITDVGQMDDSDSEAEDTDPAQPGAINGGTDPKKRNIGVRGMERLAICEDCNLAFCVVCLSSWHGDFVRCEPRDATQLTEEDQASLNFIMKNTSPCPYCSVPCQKSHGCNHMTCAQCKTHFCYLCGAWLNPDHPYQHYNDPKNKHCFQRLMDGAEGDVANGEIQFGGRRGAEQIADFWEQEAMRIQMQMNEEETW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7T4</accession>
    <name>A0A9W8Y7T4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7T4"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="260" mass="28296" checksum="190A4C3AE4E87B43" modified="2023-11-08" version="1">MATPLAELHVSATLPLSHLLTTSTNTTTTSPFTLGAGVSSDPKPKNRTFLIITPSSTLRSKHSNSYKLSLNQRRNNPAAAMAIHKAKPRVQFVDRSKQDGKKTKASAAKNNHVGGNLLREPPSVFDNDTDTDDDVRDNVPTFAKRRIPSPAEMARSGQFKSGLCGVTRSPYASDTIFLLPSPVEDGRVASEQACALPLELKLTHAQVIPPVCVAEADQLSHHLSGNPACNLLDRKSDPWPKWHAKTQSQKELPLFSHEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8E9</accession>
    <name>A0A9W8Y8E9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Rho-GTPase-activating protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rga8</name>
        <name evidence="6" type="ORF">N0V83_005144</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8E9"/>
    <dbReference type="OrthoDB" id="2155291at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000935">
        <property type="term" value="C:division septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04436">
        <property type="entry name" value="DEP_fRgd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04399">
        <property type="entry name" value="RhoGAP_fRGD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000044">
        <property type="entry name" value="Rho-gtpase-activating protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000050">
        <property type="entry name" value="RhoGAP and Fes/CIP4 domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000073">
        <property type="entry name" value="RhoGAP and Fes/CIP4 domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF17">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN RGD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="5">
        <location>
            <begin position="3"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="DEP" evidence="3">
        <location>
            <begin position="231"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="472"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="166"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="678"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="696"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="738"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="765"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="823"/>
            <end position="841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4370623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="841" mass="93404" checksum="B806A00199837996" modified="2023-11-08" version="1">MSTGFAASFWSSDYAGGLGVLFGKLQQGVQENQQILTVARMRADAEDVYGNSLNGIPTATERISGGFSRDEGASVRKAYEGVRTEMEAAAANHKKIASNIRELVVNPFGRWCDAHAARVQNSQDDLQSRIKLHDRQAEAVRKFRSQYYNKCRLVEDLEEEDKLAFQDPQSEAAQSPKAKVPTIKMTEPEDNEQEPIDLGDETYQPEQVKKILTHMLDNVKVGEVKVPIIGTYQNCSSGSDITEYIQKHMAASSVSYAERIGQDLVNHGFLRLIGNVGNTFANSSRMNYQWKTKTFQLTGLPEKKQPLTRSSTAMSTSSADNFTVDSPVGTVQEYLSGWNPLNNQFPNETPAERLRREAHESDERYKASVKKLDSLRCNLEEAMVDHLKFMERCELDRLRAIKAVILDFSGAISNVIPSLQSTVDKMMLFQETVQPLGDLRYMLENYRTGPYLPRVTTYENYYNSVDEQTFGVDLEARARSDKKRVPVLITTILTFLDNHYPDLEGDEARRGIWLVDVPLAQTHTLRAEINTGKRFSDEVLEKYDIPIVASVLKLYLLELPDSLVSSHVYEIIKTIYSTTATDVSEDTRVSVLQSTLGQLRLANIATLDAICTHFTRLIELTSADDVYVTALANALAPCILRPKQESSLTMNERYSYRLVRDLFAHKDAIFGELKRASTLTHSASGAQRPRAISTDESNRRANMEERQRAIAAQRSPRATSPAPGSRVTGSHRRDRSQTRFPVNTASPTEARKSNGNRGSLEVPGSDDSNADGTTGTTNGTTDASASSGAVEHPAPSSTRVPLPRKHAGSLTRGNRDSTGSLRSEPDAAPRGVTLEDKPMDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8U1</accession>
    <name>A0A9W8Y8U1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">gamma-glutamylcyclotransferase</fullName>
            <ecNumber evidence="1">4.3.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_005026</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.9" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8U1"/>
    <dbReference type="OrthoDB" id="2017317at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003839">
        <property type="term" value="F:gamma-glutamylcyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017939">
        <property type="entry name" value="G-Glutamylcylcotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12935">
        <property type="entry name" value="GAMMA-GLUTAMYLCYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12935:SF0">
        <property type="entry name" value="GAMMA-GLUTAMYLCYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="361"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="85"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR617939-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR617939-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4370505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="398" mass="44724" checksum="1739E566D5453349" modified="2023-11-08" version="1">MIAQSDTTMSQVIQADATSPSCFAKLANRLQVLRRRSHAPKRIFARPAQPSRERLDASLTDAPLDTEKLALLDGVAQEKQKTVLYLAYGSNLCNETFRGVRGIRPLSQVNVLVPSLRLTFDLPGIPYSEPCFANTALRVPAALDQHQEQLRRQDYHKDRWHKGLVGVVYEVTLSDYAHIIATEGGGSAYHDILVDCYVLPKADVVPSVPDSMPFKAHTLFAPVQNDEGDSQQTDRATRPDPSYAQPSARYLKLITDGATECDLPAEYQDYLHDIRTYTITSRKQAAGKALFLSIWMPFVLMVFTLSRKLQDKKGRAPGWLGQLSAMLFVGMWLSYDHLFKKTFGDGERTVGDEAMRGAKIKSKERPDQQSLLEATEDDDMLEQETRDTRNWRKFGGIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8W0</accession>
    <name>A0A9W8Y8W0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_004396</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="423559at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000053">
        <property type="entry name" value="SNF2 family helicase/ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF14">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="511"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="1080"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="93"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="422"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="458"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="826"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="909"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1256"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="154"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="186"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="458"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="469"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="839"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="909"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="997"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1020"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1038"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1268"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1289"/>
            <end position="1299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4371180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1299" mass="143535" checksum="A521A49C3334E221" modified="2023-11-08" version="1">MAPSSPDKPNDPLLGAQSKLTDNNPSSPYSSPTPRTAIKVLHLLRIASGLACLIAPQYTCALFKYRVPAEQALLVRMFGVREVVFGELLTSAEDKDAPDGGKSPRKHRAPFQPVDTLTSAQKPSFRPASAQASKRCYLDDDNADSTRDSDDRGQSTSFLGNSSSLFDDSELLPSPRNMVREREAPAPQPPRNEAPAPAPKPLQELRTAAPQFGQTSPLRNPLKKDRQDEYAHLFNKPKPGQMRPEHQQGPIRLPSQYSANPSRPPAPPKQSTSDRLEALNRPFKAPAPAPAPVGPRYSSASSTGSDMFEIPASHFQPRQPSQGAPISRPYNAPAQTTYPPPRPMYSSMSAANDFQPVNKGSHNPFAAPRKTVILDDQDDFDPDAAIRAEGDRFGAPDMYAYVDSGQASENIKALLEGAFDEDNEKIPRTRGRKKKQQQKDDQVGKSLADRLQALAVKDEEAEAKAEAEAGVDDEEEDEEDDGTVDGLKVKLLPHQVDGVAWMIEKETGNHNKRAKLPKGGILADDMGLGKTVQAIALILSNPRPEKGVEPENKKNKILDATGKGTLVVAPLALIKQWEAEINSKVTKSHELKVLVHHGPNRTKSAEKLKQYDVVITTYQVLASEHAACGDGPDGLKKGCFALHWYRTMLDEAHTIKNRNAKMTKACYDIRSHYRWCLTGTPMQNNLDELQSLIKFLRIQPYCEMSSWKDSISGPMKNGRGNLAMKRLQIFLKAFMKRRTKDVLKKDGALNFGGKAKDGGEKPAFQIVERNVEAVVGEFTAKERAFYDRLSDRAQSALDEMMGGEKQDYIGALVLLLRLRQACNHPNLTKSNVKDDKDALTTGTKPKTPTGLETPRKAARDTDADDLADLLGGLSVATKRCDICQNALTRDNAAAGAVRCTECEADLHASAKKKKKHRKHKHKDKQEKKHQKELKPARQRRVIVDSDDEDEAGGEWLVPQDEQRADDLGKAGGTDDENADGGGDTLASVDSSISGGEDESKSNLDSFIVHDTDSDDDARPIRNKHQKRPPRIPSRGSDDETNSESESGDSGSDEDSSEDDSASETSSEIEYDASDLTPSTKIRQLLAILEKETPNHKVIVFSQFTSMLNIIEPFLKRGNYTFTRYDGSMRNDLREASLHKLRNDKRTRVLLCSLKCGSLGLNLTAASRVVIMEPFWNPFVEEQAIDRVHRLNQTVDVTVYRLSIHNSVEERILELQEAKRKLANAAIEGGKAIGKLSMKDILALFKREAEYDHKNVDDMDDPAIFGPRRVLDDGKGEGTREKRKAGSLGFRKEDSVYGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9F3</accession>
    <name>A0A9W8Y9F3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_004569</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9F3"/>
    <dbReference type="OrthoDB" id="3689075at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4371352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="202" mass="22671" checksum="F5346B8C5EF791C5" modified="2023-11-08" version="1">METEKMSGVDKFDKALHERVSDVENPSFTKNLGLKKATILRALCSIECHLIDCMEGIENRASNDGHSDFFSSRMGDVYDAAYRAVPKRGESRHHAASQLFSERLCADDGPYHAIPDGIEENWQCEKESIIEKVMKPVHEDLREIRTSYENLGGAGQIAEQDVSEVSGELLAMVKMPKDTRKKDILSWLLDCDLDPKLKPTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9H7</accession>
    <name>A0A9W8Y9H7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Fructose symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">HXT4</name>
        <name evidence="11" type="ORF">N0V83_006168</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9H7"/>
    <dbReference type="OrthoDB" id="6339427at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015798">
        <property type="term" value="P:myo-inositol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015791">
        <property type="term" value="P:polyol transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000134">
        <property type="entry name" value="MFS sugar transporter protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="118"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="326"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="362"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="420"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="456"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="482"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="80"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4369085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="563" mass="62686" checksum="210A6504F06371B6" modified="2023-11-08" version="1">MDEKDTKVEAPPAAQRSEIQVAPPAEYDAPKAKTEQLEDARGWESENLIQDLEKELEQVGYKKGFFDLEFKNPKHFTWLLVAFASMGGLLSGLDQSLISGANLALPGDLGLTDRQNSLVNSGMPLGAVAGAFLISPCNEYFGRRWAILISCILYTIGGALEAGSINYGMIVTARVILGAGVGLEGGTVPVYVAETVESRLRGNLVSLYQFMIALGEVLGYAVAAMFITVPGNWRYILGSSLVFSTIMGIGILFMPESPRYLMHKGKPLEAFKVWRRIRGIATHESRQEFFVMKVSTEMEEAEVAAGRTNRFPWMDFITKPRARRAIIYANIMIFLGQFTGINAILYYMSVLMAQVGFNDYDATYMSLVGGGALLIGTIPAIFLMETCGRRFWAIAMLPGFFIGLIIIGVSYQISSTSVALGLYLTGLIIYELFFGSYAALTWVIPSEVYPTYLRSYGMTTSTGWLFLSSFIVTYNFTGMQNAFTRTGLTLGFYGGIAVLGWFYQLFFMPETKDLTLEEIDQLFQQPTRQLVKQNAKNVAGVTNDLLHFRFRKVFVENNQRRYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9I9</accession>
    <name>A0A9W8Y9I9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_004629</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9I9"/>
    <dbReference type="OrthoDB" id="2958217at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112:SF10">
        <property type="entry name" value="TOL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4371412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="453" mass="51378" checksum="010A9FD61EB0903C" modified="2023-11-08" version="1">MCNATLLQPRNPPIIAPCIFESHWESLKSIKYAVTDFRYWDSRIFRSVCNTRAWITQERWLAPRVLHFTFDQLLWECRELEAGETFPYGLTKEAQMQSSVGFKKACTEAPTEKSTRSADETPIDDWRKLLLTYGRSEVTRKSDRLVAVAGIAARFSTILNDEWIAGLWRETLPGNLLWSVEKVRRTNKPAEETAASFVASHSLGIHRSHQVHPSERLKGARYQAPSWSWASVLGDIVPGFDTEDGAEDSMIDVVGVELTPADPEMPLGRLASGTLRLRGTLYPMAIDPPDWHTRSKKRNYMGIPPTVYITGLPKTKVAPLGLEWPEEYTKNPKYAHFLEGHSHTVHLIPDEPVKYLDLARSKYLPIRKRIYDNPKSEYHGKHLISGLALLPTGKRGEYTRMGRIDFGDDKSKLFEDGVEVQRKGEEATGLKAHPPTMDEEFYVEGEIGTLTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9J3</accession>
    <name>A0A9W8Y9J3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_004372</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9J3"/>
    <dbReference type="OrthoDB" id="2593732at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052360">
        <property type="entry name" value="Transcr_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36206">
        <property type="entry name" value="ASPERCRYPTIN BIOSYNTHESIS CLUSTER-SPECIFIC TRANSCRIPTION REGULATOR ATNN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36206:SF4">
        <property type="entry name" value="HYPOTHETICAL CONSERVED PROTEIN (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="277"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4371156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="372" mass="40843" checksum="E5083C6B2D3E961A" modified="2023-11-08" version="1">MEASSNPEYPDRCTSTGRKCDGYTQDASFGFEALVPSQLGSVRTSIPILSGLGDSVIYLEFYHHCASPTLASNFDREFWSHTVLQMAYSEPSVRHALIALSYLVKTMPGSLKHARSIPGVDSHTTLLVHYNKAVRSLVERATQSSQSVEVGLITCLLFICIEFLRGDYLVAFTHLQNGLKIIAEWQQTPQQVPNDDLHYRAHLLTCHESWLQAMKVLENGGNLSKQDSLVASSLRLSHYATYIALAGATDIYQMSFDAHIADFEALNHHARIVLDSMNLPSSSPGTPTTSPSSSASSDPKDPAAHFTFEIALIPPLVSKSFHLTISNDLLKDPPYSTSLQFAAVAPRQGANRSLFSHGIRHAKPFGTPNNML</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9P6</accession>
    <name>A0A9W8Y9P6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tail specific protease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_005425</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9P6"/>
    <dbReference type="OrthoDB" id="27214at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056186">
        <property type="entry name" value="PDZ_CPAF-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052766">
        <property type="entry name" value="S41A_metabolite_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005151">
        <property type="entry name" value="Tail-specific_protease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37049">
        <property type="entry name" value="PEPTIDASE S41 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37049:SF4">
        <property type="entry name" value="RHODANESE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23658">
        <property type="entry name" value="PDZ_CPAF_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03572">
        <property type="entry name" value="Peptidase_S41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CPAF-like PDZ" evidence="3">
        <location>
            <begin position="125"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Tail specific protease" evidence="2">
        <location>
            <begin position="321"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="677"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4369663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="735" mass="81682" checksum="31AB25A07036F500" modified="2023-11-08" version="1">MYPKATIALVPAQAAEDCLKSVPIDKTEDLALIEEMKLYINWQSNLAYLLDPPEGYAGDRVDTHDEIDRITNDLKNDKYKDEYSLMFDLQSTIAKSYDFHFVFAPEITQIFNFRRGNVGMGLVDEFALVSVSKDGKELPKLYNYYDVIVGEEEGWTPSPVSQINNQSAEDYIQQWSTNFVYHEDHARYNMLFPSQPQQSMGNRQNQFGRSRVPDGDYTTVLHENGTINKYFNNAIVQMGDFEGIEDGDSFSAKFINRGPPQNSSLKRGEKRNLLEMTDTVKLPTKRQTTVEPSPTGFPTPEMYHSEGIIGGYYLEDQGYTDVAVLSVPSFQPTADNSTLEFQDKAGSFLQAASKAGKKKLVIDLRGNGGGSVFLGYDLFKQLFPSEDPYGASQFRANEAFDEVGKSLSDLYDQGNITYEKVLADYHVNGTDSLMAVAWQSIFNWRIPLTTDNKNFTSWEDFFGPNKRHNDSWTTVARKDLNNPFSDDLGMDVTGYGERIKALNTNQPFTSDNIVLLQDGGCGSTCAVFSEFMKSQGNVQQVVVGGLPKTGPMQGVAGSKGSQVYTWTQVNSEASKVYNLLPDQQEALNKTDLGALVNAQRPLMRSAIGSFNRTQSSINLRDNIRMNDTTVTPLEFVYEAADCRFFYTGDMVREVEAVWKKAADARWGNTKNVCVEGSTGDPSSLSGGATPWEGNPDITTKAEKVETVTKTNAAVNMGGSGFLTAATTALFMVFML</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9Y5</accession>
    <name>A0A9W8Y9Y5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acyl-CoA dehydrogenase/oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004857</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9Y5"/>
    <dbReference type="OrthoDB" id="435240at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="1">
        <location>
            <begin position="12"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="150" mass="16811" checksum="9FAA03F746D875E6" modified="2023-11-08" version="1">MNFLPTNLPEWTDSQKSVREAISKICLDYPDDYWAEHDTEHKFPWELYKQLADNGWLGICLPEEYGGSNLGISEAAIMLQTLAESGGCMNAASSVHMNIFGLEPVVKFGSDEQKKRFLPPLIQGKDRACFGVTEPNTGKCSPYVLKAERH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAC6</accession>
    <name>A0A9W8YAC6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="1">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_003788</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAC6"/>
    <dbReference type="OrthoDB" id="269822at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08598">
        <property type="entry name" value="PI-PLC1c_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF82">
        <property type="entry name" value="PHOSPHOINOSITIDE PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PI-PLC Y-box" evidence="3">
        <location>
            <begin position="395"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="165"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="204"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="363"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="563"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="204"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="363"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4372015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="616" mass="68300" checksum="514EBDAEBF78B2BD" modified="2023-11-08" version="1">MTTAAKDEKLGAPKLDKPQLQAGGGTASVTSRHQITDALSPLLVQHLRQEYDVLVKKYQLGTKEGLSRWLTEEQQCSAQDAELLADGSFSHFANYFLSGAANVMKPAQPVDESYPLSNYFISSSHNTYLTGNQLSSESSVDAYKNVLLRGCRCVEIDVWDGEELSDSSSDEEAALGNPGASARKEGKSKSGLGIRKRLELKFGRRRASSEEKAKEKETTSAAQDSDKPIQPWRSTSSTMRAEPRVLHGYTLTKEMSFRAVCAAIRDYAFTVSSLPLIVSLEVHTCPEQQEIMVELMNEYWKGMLVDLPLDPSQTSEQASLPTLKDLENKILVKVKRASHAKPAKVEANLSTLQAPAEPLKHTTSAVSVASSATSSDEVPEGEKKPAPKPKVIEALSKLGVYFGSYHFKGLDAPESSIPTHVYSLSEKALMEVHETNPSGLFRHNKHFFMRAYPKGLRLNSSNLNPSVFWRAGVQIVALNWQRWDGGMMQNEAMFAGTPGWVLKPEGYRSTSQEANQKAAIPHHNLDLGIEFLAAQDIPLPPEEDDPKDFKPFIKVELHVEKHEERNAEPIPGNGKSQKGEYKKKTRTYRTTHPDFGREIVKFEGVQGVTEELTFVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAL7</accession>
    <name>A0A9W8YAL7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA polymerase II assembly factor Rtp1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_003134</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Tango6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAL7"/>
    <dbReference type="OrthoDB" id="39591at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019451">
        <property type="entry name" value="Rtp1_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019414">
        <property type="entry name" value="Rtp1_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039600">
        <property type="entry name" value="TANGO6/Rtp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20959">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 6 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20959:SF1">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10363">
        <property type="entry name" value="RTP1_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10304">
        <property type="entry name" value="RTP1_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA polymerase II assembly factor Rtp1 C-terminal" evidence="3">
        <location>
            <begin position="693"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase II assembly factor Rtp1 C-terminal" evidence="2">
        <location>
            <begin position="1001"/>
            <end position="1031"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4372843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1081" mass="118240" checksum="6C2C08982E9B69F7" modified="2023-11-08" version="1">MGAVEDAVDAAASFIGPFVDKDRKHSPSAIDAAQLVDQALKHLQAINSADLAADPNAPYDASLAGVAYGLLDLTTLMGILPNLSPGVAFSQRPRSVLTALITVPSSRNEELLSRVVTVLIHILGQRGSGVQPLVSQRILPDIFSALIELSFSPELTQEIHSRFGSMYKKTVAEVSTSQLLPILTTFLQQSLPAWLKPVISQELALIPLRQHGVRHTIEFLSLSYLSKNSRVPEDASGPQSQIPIPLEAITQTSRLLVLPPTGISQDDWLCRLTPQLQKLLDGTDGRELSRAAGQIIAGGILSKKSTGAPGTVGWELFALPLLQAIYPKDTQKGPSRGSTADQVIVQDQDLCLALKRLSTIASSYSHAGILRRLVGPLLLPLWALLNHAKARPSLDKQWTRLPQNILTRYMTIACNVSQVDSIASNLFWDGDVLWTFGPGSHGGIEIRRRGDDNHGLEEMDGILSRIGNLDSRVSLLVSMLADAKIQDDIAGSIFLQVTKRWLSSEQHLETQLTSNSETDPLAALTNAKLSEALAKKFQDTFAKSPQHIIELMGQLIQNFVKEHQGRVNGLAKSQLPSRMLLGNIVNKRGQDLAGREGDEDRIVDEDLVSFALSIITTIAASSGFKETSIPNRTLESIIPSLAYLSRQHDQLPISSVVTNSATNLLDLVSPSASTATTTTTMTDPLSIHRSVMKTTLSDLTSPEPPNRTWALNSLRALIQNPAAFPVIDVPSTAHLLLSASLADSESYVHTAAIPVLVELAICAPNPVIRILTDAFIDVDERSLKLARGRQTEEKERELQDALDFRLRVGQVLNDIILDHRFTGPTHDVALWYRCLKGIIEACLSLASRRGQRTKTLSTRTQLAAAEQKLQEEGEAAWGGPIPNLFEPESEDPSERVERDALAKIVQGWKDTGVEEDVRIRTSALSVLSTVLENQLRSLNQPTIDAVLQMVLLILSMETSEEKAILRRAAVLVIMGLLRGFDSALENGEDVPVRLSINQQNEVERLVKWVRDDDVDALVRDHAASVVEGLETLRLKELYKVRDEGLRLGPDLGLEGELRGLDVKPDLEGKAAKRKLVMEEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAN6</accession>
    <name>A0A9W8YAN6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004443</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAN6"/>
    <dbReference type="OrthoDB" id="504210at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="288" mass="32486" checksum="D7023AD88944496E" modified="2023-11-08" version="1">MGRTKQKPLPLPKPTALHPLPPGTQAILRSGAQAVAYHIPVAPTSPANPNLKSTIALPRYSKWTSGLHFHTAHTEYLRLIKGSIFVELNGELKILSAKAGGEVSWSYGEVSKKGLEITIDKYARHNWGRADEFLNSTLRRRIVGAYKTISPEDCLEEVIVEEWTDPADITKSLFFWNLNGVIAAAEYTPLSSGQSLVKFCLRGWWIPFQLFVIFWDLDNWPVFLELSAFSTPFLGNDVGAHFDHLLEYLITFAMLFVARTLARFTGIQSVSKERTPHDLWQAYRNSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAW1</accession>
    <name>A0A9W8YAW1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">2-hydroxyacyl-CoA lyase</fullName>
            <ecNumber evidence="9">4.1.2.63</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_003927</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 2-hydroxy-3-methyl fatty acyl-CoA = a 2-methyl-branched fatty aldehyde + formyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:25375"/>
            <dbReference type="ChEBI" id="CHEBI:49188"/>
            <dbReference type="ChEBI" id="CHEBI:57376"/>
            <dbReference type="ChEBI" id="CHEBI:58783"/>
            <dbReference type="EC" id="4.1.2.63"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:25376"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>an (R)-2-hydroxy-long-chain-fatty acyl-CoA = a long-chain fatty aldehyde + formyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:67444"/>
            <dbReference type="ChEBI" id="CHEBI:17176"/>
            <dbReference type="ChEBI" id="CHEBI:57376"/>
            <dbReference type="ChEBI" id="CHEBI:170012"/>
            <dbReference type="EC" id="4.1.2.63"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:67445"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.63" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAW1"/>
    <dbReference type="OrthoDB" id="10006023at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106359">
        <property type="term" value="F:2-hydroxyacyl-CoA lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001561">
        <property type="term" value="P:fatty acid alpha-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02004">
        <property type="entry name" value="TPP_BZL_OCoD_HPCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000071">
        <property type="entry name" value="2-hydroxyphytanoyl-CoA lyase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045025">
        <property type="entry name" value="HACL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43710">
        <property type="entry name" value="2-HYDROXYACYL-COA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43710:SF2">
        <property type="entry name" value="2-HYDROXYACYL-COA LYASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="13">
        <location>
            <begin position="10"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="11">
        <location>
            <begin position="185"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="12">
        <location>
            <begin position="387"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4372154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="583" mass="62341" checksum="09755B6D9CEE8547" modified="2023-11-08" version="1">MPSAAQAPVTGANIIANTLKDLGVTSIFGIVGVPVSDIAEQAINIGIRFIGFRNEQAASYAATAYGFLTGKPGVGNASENNFPLLLLGGSIETHLVTKGGFQELDSISLLTPHSKLSVRPPSVDSLPSIIANAYRCAFYGRPGTGFVDLPADIINDPLTSDDFEYIHETPRVADPPKGASDVQRLDKVVNLIKGAQAPLVLIGKGAAYGHAENVIRNFINRTHIPFLPSPMGKGVVPDSHVNNVSSARSAALKSADVVLIFGARLNWIFHYGEAPKWNPAAQFIQVDISPEEMGRNAADAELSLVGDVAVVVSQLAQRLSGWKHEPLGSEYIKDIDIAKQKNEQISALAAKDSSIPLSYGRAFDVIKKVLHTLSPPSDGGICYIAEGANTMDISRSIFPLEHPRLRLDAGTYATMGVGLPYAIAAWEAYNGPNPQASSGSAPRKKIVAIEGDSAFGFSGMEVETMARMGMDVLIFVINNGGIYFGDSNSAEDWTTKQGKTKRNEPGLRSWALGWEVNYQKFAEACGGIGFLVRTPEELQNATVAGFNAKVPTIVNVIIQSGKVEKATFGWQVSPKRILKSARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBJ2</accession>
    <name>A0A9W8YBJ2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7707 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_005239</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBJ2"/>
    <dbReference type="OrthoDB" id="2121879at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056124">
        <property type="entry name" value="DUF7707"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38118">
        <property type="entry name" value="ANCHORED CELL WALL PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38118:SF2">
        <property type="entry name" value="CDP-ALCOHOL PHOSPHATIDYLTRANSFERASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24808">
        <property type="entry name" value="DUF7707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040975474" description="DUF7707 domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7707" evidence="3">
        <location>
            <begin position="32"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="141"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4370718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="202" mass="20253" checksum="57952E2F5C4A25F3" modified="2023-11-08" version="1">MLYSTLIVAASALAGFASAQNSTANTPIACCTVPVTQVPESQRDDWCTANENTCVELCGGQGDIASNGNECTASDLSYTCKCSNGTDITSNLQSYQQTVPGLMCRYWFDACINATGTDASLQFQCISARDTECGNQTVDDSSSSSSSASASASSTASKTSGSSSSSATSSEAAASSGAAATLAVYGTPALAGGLLALFGLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBJ9</accession>
    <name>A0A9W8YBJ9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">F-actin-capping protein subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CAP2</name>
        <name evidence="12" type="ORF">N0V83_004236</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 10">F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the F-actin capping complex, composed of a heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Actin patch</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the F-actin-capping protein beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBJ9"/>
    <dbReference type="OrthoDB" id="9979678at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008290">
        <property type="term" value="C:F-actin capping protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.570:FF:000001">
        <property type="entry name" value="F-actin-capping protein subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.210:FF:000005">
        <property type="entry name" value="F-actin-capping protein subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.210">
        <property type="entry name" value="F-actin capping protein, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037282">
        <property type="entry name" value="CapZ_alpha/beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042276">
        <property type="entry name" value="CapZ_alpha/beta_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001698">
        <property type="entry name" value="CAPZB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043175">
        <property type="entry name" value="CAPZB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019771">
        <property type="entry name" value="F-actin_capping_bsu_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10619">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10619:SF0">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT BETA ISOFORMS 1 AND 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01115">
        <property type="entry name" value="F_actin_cap_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00192">
        <property type="entry name" value="FACTINCAPB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90096">
        <property type="entry name" value="Subunits of heterodimeric actin filament capping protein Capz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00231">
        <property type="entry name" value="F_ACTIN_CAPPING_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0117">Actin capping</keyword>
    <keyword evidence="6 10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4372462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="278" mass="31423" checksum="49409B1994A4A46E" modified="2023-11-08" version="1">MADPIDSALDLLRRLNPKDVKRNVDHIIQLNPALEEDLLESVDIPLTIKKCSKTKRDFLCCDYNRDGDSWRSPWSNEFEPAIEEGVTPSDRVRKMEVKANEAFDVYRELYFEGGISSVYLWDMDDGFAGCVLLKKAVNPTPKSSGSWDSIHVFDAQDRARTAHYKLTSTVILSLGTDSEALGGLDLSGNMVRQVEADMAVEDDTSHVANIGKMVEDMELKMRNLLQEVYFGKAKDVVGDLRSMKDFKDWHRIFADAIHRSPVPQPNQPRSGNAERDDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCU5</accession>
    <name>A0A9W8YCU5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">O-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_003731</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4371958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCU5"/>
    <dbReference type="OrthoDB" id="2410195at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF12">
        <property type="entry name" value="STERIGMATOCYSTIN 8-O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="6">
        <location>
            <begin position="110"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="5">
        <location>
            <begin position="212"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4371958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="50032" checksum="3DE08B149B5DF469" modified="2023-11-08" version="1">MPATLTNGHTNGHTNGHTNGYKVEKSSSSSTDLLTLALDVVNNAANIKSLLAAQNLPAPDFSPDSVDLPDTPELAKLRNQLVASLDDLRQLALGPRRTLRSLICFSNDLAAFQVAFEFGFFTIVPESEDGIALADVAKQAGIDIGRTRQVLRLLCTHRVFREVSEERFAHTAASAAFARDDNFRAAGGYGMDEMFKAASSTSDTVRASPMKSDLEHSPFRTRLGASMFEYYEQHPKQANRFAKAMAGVASVDRKPSTLGDMFPWDTLKGTVVDVGGGSGHIAMALAHRFPHLNFLVQDGVQDMLTQGERLLEKEDPSVASRIDLLLQDFFEPQPPMERLAARGPVAGFFLRHVFHNWNDNDSINIARALVPALEAADPETPLIISERVLPRLGEGVLLHEERSMRQMDMMMMVGLGAKERTLAEWQALFSAADERLVVKKVHGEAASAVLEVVLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCX0</accession>
    <name>A0A9W8YCX0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001800</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCX0"/>
    <dbReference type="OrthoDB" id="5421601at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4374725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="509" mass="57362" checksum="40A6318A18247202" modified="2023-11-08" version="1">MRLLDLPPEILDLVINLTLPNGLENFVLSCKTIYGRARSQIQHHNTLKRRWRHTTNDSYPDSPADTLGIMLGISEDPLIAEYIECLSLWDRRPWDPRPGEILPMVSGRSREYWEDHENHDQMQGIKNLLYTAEYFAKADPEEWWGRMINEEDITSGEDADNLYATIALLSLLPNLKTLQLPARWHEVRSGESAEALVPSVESLVSVSNRDGYTLKPLASLETLLPFVEEGYDVRAGLQCLQPFLVLHGIRNLYAVSCVAVNEGWGGIPLEWPQSGLKSPLTRVEFAYCCMDTAGLGVLLSNTPCLTIFKYSHQTKWDGLEFDWNPGEFLETIANYCGERLLELAITIDELHGEVINGLSSFMRFPKLQKLEVDVAAFCGPPLESGQRLGRNATLPEGATAWAHIDIPCMGDMLPESICELQVNTDFPEPSEQALRSLFKNIRDRRLDKLLLLGTTILRQYASGTAREIAVNHGVILEAFDEDVAAPRPRPMMPQWKREFDCRVGGIVMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD56</accession>
    <name>A0A9W8YD56_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mucin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD56"/>
    <dbReference type="OrthoDB" id="5380370at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="160"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="54037" checksum="FAEF1985FB275DC6" modified="2023-11-08" version="1">MRRKARVLNPPLQQAEFSALPVQVQRKYFSSLERLQIQQQALEEPTPTSGRSSRASSSTPFRPSLTLGSRRRQRKPENVVHGRDIAQADVDFFLSLPQKLRKQHFSREEQVLLLEKHRQTSPPDAQDRERAQQRFNDFQFDFSSIDDGASERGRPRRRSSSASPPVSPSAPSFGLPIAKEKERNSNRPSDRLHLDIMGPSASASMSSAGSIRRTMSLTTPPVRQSSSAAVLMADPPLFAMAPSTRHDRAHSASLSGRRSSHTPTAPVFDPEATHYQDPEARKKLRMYLASPQKFDEAIEFGFPSTGTNETIVPHYQLPPITSHARKFSRDMHTFLKDGKLSFLEDHSSENQGLDSDEDSVADMESPATPSSTGLSFRLHSRNVSQPKFSMDSSGLSPVHPVAGHLNREMTLRMTLTRPDLRADDDQLYGWQSSASKAVKDDPLALEELLLTDDMTGTKGAFYVKPKSRDSLVTRLLKRASLKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD92</accession>
    <name>A0A9W8YD92_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_003956</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD92"/>
    <dbReference type="OrthoDB" id="298012at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43333">
        <property type="entry name" value="2-HACID_DH_C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43333:SF1">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE NAD-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="4">
        <location>
            <begin position="60"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4372183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="178" mass="19578" checksum="1CDAD8FC5364EF1A" modified="2023-11-08" version="1">MGMDVIAYTASPRDTPESKKDHGYIVPGTGDPDGAFPSAWYSGTSKEHVHEFLKQDIDLDATKHFLSTEEFELLHKSNTNGKGTYVTNIARGQCIDQKALITALEKGHIRGAALDVTDPEPLPADDPLWDAPNVLITPHCSGSTDVYADRGFQLLIENIKRQKSGGKLINEVNRKRGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDE0</accession>
    <name>A0A9W8YDE0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDE0"/>
    <dbReference type="OrthoDB" id="3694353at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="330"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="499"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="628"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="740"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="451"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="746"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="773"/>
            <end position="789"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="881" mass="99198" checksum="B0522A44DF1B7471" modified="2023-11-08" version="1">MATLKRSRESEPEEPRKRHRDEDYKAGPEIQYEQSVLNRGGYEFIQPVVSAQTPLESQDSHSRNTASTPIAQGIRYSNNEANIASPHKHNAQVIAQTSLIPSSWPTPVLGKRQVLKIKVWERNSQCDSPQDASNQRDQVTSDTAVVSLADGPRSGDIADGTRHTPYMFDFESIPREVVRRTLQWIDGARDQVGQSRVRKVPGIPQDYIKDFANEPGPYTPNDIWKLRHLGYWARYQMYLEFQKLNDGIGLIPFQEESFWDIWEELQTKTKELQHAEQIWYRKYKDREFEEEDTRKDAEKKALSTWNQLLIWQSETLLIDHQNRRCRRQAGHVPKNGSVVGAKRKAGELGGDTGSSSKKPRIEDGKVDEDNFIEPAGSPTPPPHARILTPPPLLFETNGSAIGTKQKAGGLEDGDTGGSDEKPRNEDDKVNGDNFTEPPRTPTPPPLVFETNVRDQMLRHKQGKDVDETPTYTAEHKEAGTGAVSKYPWMAATQEAVQKIQKKDAGKKKKSRPQARDLAEAEDNEKLLYWKKKYPSDKVNLPDHPFPKVEDYAANAAGRYQCFHKDFACSDRSCKHVCCKEGYDTKGLKKAIQKAIQTYKSQVERKIEDGDLDRRHKIWEKWFNSSLKAQKDPGQTKTKTTKQSGKVSQNEAGDMPNIANATTPEAQLEVAPTSQPRVNAAAVAAATLAAQQAAEREQKLKADMKMVDQGYKAACRRENRKKYPDFDLLNAWWAAARKELQGLPLTPEERALRERPEGDPSLREKRPIFKAIATAQSTAIQSPEQLQETSQQEDDGLEQAILDCLFDDSPDGPEAGGQQPPQEEGLDPPFDVPLHGAEQDAGQPQQEGASFGPSFEEAPPGDGRELDDRDFDEYLLNMFQDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE33</accession>
    <name>A0A9W8YE33_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_002068</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE33"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000064">
        <property type="entry name" value="MFS allantoate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF97">
        <property type="entry name" value="ALLANTOATE TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G14700)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="380"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="415"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="443"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="61"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4374989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="507" mass="56407" checksum="3B17299F3C348955" modified="2023-11-08" version="1">MAEKHPDISPAREPSVDEGQLAAEKQMRDIGAELYLEVRQYSREELDAERKIVLRKIDWVIMPMICVTYTIQFLDKLSLNYANAYTLTPDLGLQGSRYSWVAAIFNFGYLFWALPANYLIQRLPVAKYTGSMILLWSILLCCHVAAKNYAGILALRFLLGMFEASISPAIMNIVSMFYTRGEQPLRMCVFLAFNGMATMVGALLGYGLGHAHSPNIKTWQLIFLVIGLLNFVWAWVFLWIMPDSPSSAKFLTHKQRIVAIDRVASNMIGVKTKQFNMAQAIEALLDFKVHCLILIGMCCGVINGGVSNFASALIKGFGFSGIYTTLLQLPTGAIEFVVVPICGLAAGYFKNTRCLILAFICLPPLGGLLGIRLTPLSHRWTLVGCTWLQYIVGAPVILSWNLLTTNIAGHTKRSTANGLWFVFYAAGNIAGANIFFAREAPRYFSALTGLIVCYCAMVVIALVLMVYMAWENKGRNKKYGHGEDEQAIQDGFTDITDGKAKHFRYAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE74</accession>
    <name>A0A9W8YE74_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Poly(A) RNA polymerase mitochondrial-like central palm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE74"/>
    <dbReference type="OrthoDB" id="273917at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010605">
        <property type="term" value="P:negative regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF15">
        <property type="entry name" value="INACTIVE NON-CANONICAL POLY(A) RNA POLYMERASE PROTEIN TRF4-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="2">
        <location>
            <begin position="163"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="503"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="504"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="569" mass="64900" checksum="D4348A379609590F" modified="2023-11-08" version="1">MRPLSSRLICRAHNRFSPSTVLWQHFVLPNRPLLHRQCLSSSTTASAQDPAYDDGQLDPPQETSELKQAEAETAEVEEENIEPVPKVGKGTKNVQKEYHSRQISLTKSEKLLAVARAAFNDAIEYDAVVVKPKRIVTSREERSLPWCLAQEERTMPGIDRLAVEIERFYEYAKPSKFESIARRHLIEQVRSHVRKVFPDHVIEVFGSERTGIALATSDIDLRLIRQSKLVDPVKPKLPPTPEERSQGLRDLHKLYQSNLKQHKAYMLSALRHARYPLISLQDRQSGLVVQIVLANDTSVSRVIMQDYMEQYPYLRQLYYVVKTAFDVRGLSDVFRGGFGSYSLFMMVVASIKHNPHPRNDAAGGLINFLKFWRDFDTREHGISIEPVEVFAKGASPVMTDTIKAKLKEGTVQPLPSYMLSLRDPADQTNDLGRKGIAIKHVQATFRSLVKELERDIRANERHSILSPLVGPSYLLDESQRFKLVHYGKRLVTQMQKTLSAKATALRETEKDTGMDRRQEDETPTLARAARDREWQRSVDEVNERLRLDVEKASASELKEASLGIAPYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEL5</accession>
    <name>A0A9W8YEL5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acetyl-CoA synthetase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_002075</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Siderophore biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEL5"/>
    <dbReference type="OrthoDB" id="6509636at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05911">
        <property type="entry name" value="Firefly_Luc_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000003">
        <property type="entry name" value="Long-chain-fatty-acid--CoA ligase FadD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF149">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="4">
        <location>
            <begin position="28"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="5">
        <location>
            <begin position="446"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4374996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="61912" checksum="2F4E8B232DD5560F" modified="2023-11-08" version="1">MPSKSPFPDVEIPEVDLWDLIFDRKDRDFSDNKVIYRAIDSDRKYTFADVRNLATLFGEGLLNLWDWQKDDVLALYSPNDIDIPPVIYGAFFAGGIVTPANPGYSADELAYQLENSGAKALVTTKKFLDTALKAAEKVGIAANRVILLDAAEKDESDRIRHWTSIRKTPGAQKYYRRRAKPGDLAFLAYSSGTTGLPKGVMLTHRNIVADLLQIHGAVGKWYSTSEDKFLGVLPFFHIYGLTGLVHQMLHRGIELVVMPAFDMETFLKGIQDHKITFIYVAPPVIVRLSRDKMVDKYDLSSVKMITSGAAPLTKELVDTVHKRLNIKINQAYGLSETSPMTHTQPWDEWYTSVGSVGKMFPNMSAKYMSADGKELGPGVPGELWLSGPNIFKGYWKNIAATKDSITADGFFKTGDIGFQDEQHNFYITDRVKELIKYKGFQVPPAELEGKLMENELVDDVAVIGVDDELQHTEVPRAYIVAAAPAGAQAKRAKAGETEAKVIVEWMAKKVANHKRLRGGVVFVDEIPKSASGKILRRVLKERSKGDVALGIAPKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF31</accession>
    <name>A0A9W8YF31_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Haloacid dehalogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002307</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF31"/>
    <dbReference type="OrthoDB" id="40579at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051540">
        <property type="entry name" value="S-2-haloacid_dehalogenase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01493">
        <property type="entry name" value="HAD-SF-IA-v2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43316:SF3">
        <property type="entry name" value="HALOACID DEHALOGENASE, TYPE II (AFU_ORTHOLOGUE AFUA_2G07750)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43316">
        <property type="entry name" value="HYDROLASE, HALOACID DELAHOGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="26228" checksum="7A796894875A924E" modified="2023-11-08" version="1">MTLEHWGLFAQQWRDGYKQFTRKLAEDPALPWISVDEHHLTSLKELTVKWEIEGLWTDEELRSLSLIWHHLEPWDDSAMGVALLNRLFYTCTLSNGNISLLSDLRAFSNIPFTHLFSAELFGTYKPSPRVYIGATERLNLPPNQCAMVAAHLNDLKAAKQNGLQTIYVERVGEEEWSREDIEKAKQEGWVDLWVSLGDGSKGFITVAEKLGIDVTSSSQARRLSSSAPIGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFG2</accession>
    <name>A0A9W8YFG2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002971</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFG2"/>
    <dbReference type="OrthoDB" id="47801at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="384" mass="44080" checksum="3723C7058A9507D9" modified="2023-11-08" version="1">MIHVKPSIDVQNRSRKLVAHLAMMLTDLRIDTQYTEHGEPLTDESRTTEETQERDRRRRFIAEFAPHLRKVEQIKLEGGIPRDEKREILRALHWCPLKKVVMIGLSFPAGNTWGSQGFQLKALDPGQNIDSNINLEDEDLDGILASYRRGFSISRGFQFEPDYGWPAQAPLLQTIALHHASTVEELKICGYNGCPILSFQTPMTAPLLNGLRHYDNLKQLVISLWLLTWYEDSYRDTEIIQSWLDTRSPSSTALAVVTPPTSPMREHPVDPGQFPNFNPRVAPRQEFNRWAVALKTRFSPSALAYRVARDMGPYLSPVAKERPGGVKVRASFCLGVREEGRSASDIFDLEIRIGQDDQVLEYIGPREEGERGRWWTKLEERRWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFH1</accession>
    <name>A0A9W8YFH1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001054</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFH1"/>
    <dbReference type="OrthoDB" id="9978720at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12809">
        <property type="entry name" value="Esterase_713_like-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050228">
        <property type="entry name" value="Carboxylesterase_BioH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43194:SF4">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43194">
        <property type="entry name" value="HYDROLASE ALPHA/BETA FOLD FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4375777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="323" mass="35548" checksum="C1A7D45A80350F1D" modified="2023-11-08" version="1">MIGQIYVEKLSPNPAPECPPLPIIFIAGAAQSGTNFLDTPDGRPGWAAYFLSKGHTVYLSDQPSRGRSFWYPGQGTIGYIGGPDSVSDIFTDVQNNGNEWPQAKLHTQWPGTGRIGDPTFDQFYRSQLQFQTDRFISEEQNAQAYSALVDVVGDCYIISHSQAGAYGWRVGDMRSDRVKGIVQLEPSGPPFTLRPPFGTDPAFAFGLTDLAIGYSPSAGVNASNIETTIEPAIDADHNDCIMQKSPAKQLTNLAKIPELVVTGEASFHAPYDYCTVKYLEQVGVDVEYADLGKEGIHGNGHMFFMEKNNLQIADRVYEWLKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFW1</accession>
    <name>A0A9W8YFW1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">DnaJ-like protein xdj1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">XDJ1</name>
        <name evidence="10" type="ORF">N0V83_001590</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFW1"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000001">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000003">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="8">
        <location>
            <begin position="53"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="9">
        <location>
            <begin position="194"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="6">
        <location>
            <begin position="194"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="424"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="424"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="436"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4376307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="479" mass="51687" checksum="8BB833C02B3203E0" modified="2023-11-08" version="1">MRAAEVLASSAASEMPVIDETLLPLLDKFGALPRLHLLFLLTYTNMNDVEEVDLYEVLGISNGATKIDIKKAYHKAALAHHPDKVAEDGREEAEIRFKAAKQAYEILSDDEKRAMYDTHGMAAFDPSRGGGMGGDPDIDDIFAQMFGGMGGFSGMPGMGGMGGMPGGPGGRNVPRKGRSVEQEYEVSLEELYKGKTTKFSNTKNVVCSLCKGSGGKQGAKSHSCGVCNGRGAKAVLRQVGPGLVTQETVPCGNCQGSGQIIPEKQRCKKCKGKKVVETKNVLELYIPRGARQGDRITLAGEADQLPDQEPGDIVFTLTETPHDVFERAGADLRAELKVSLVEALTGFNRVVLTHLDGRGLQLHVEQPNGNVLRPGQVLKIAGEGMPMKRSDARGDLYLMVDVEFPEDGWLKSDAALQKVRDALPQVEKEDEKHEEVEEVDVEWDADMEEFGAGSGDPRAAGGEWEDDEEGEGGPQCATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG57</accession>
    <name>A0A9W8YG57_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001418</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG57"/>
    <dbReference type="OrthoDB" id="3163292at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4376137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="260" mass="28825" checksum="A30B4088A2599CA7" modified="2023-11-08" version="1">MTMTQQTAFASVSQSFIDRTITLAGFTILRLVRSPLAQHLDLAAGEKAFFQAVQFLKNVALQQGDIGFRTALIMNDLWGSSRVFRRKDGRIESLGLRLRTRLSMSVSYDMFWYWREEFGHMQNPYNGEETIGASNDATQPSTPREYFHLSLLSTSLMGNLAYVSNQAQGLPNSSAQSQAPISKFDPSLVNPQLQPDPTVMSFDGWDGSEYSAPPMLDQFPDYDWAAGFDFSNSDFPTIPVGPMNTIVPGGNPVGMGYTFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG68</accession>
    <name>A0A9W8YG68_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Delta(24)-sterol reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG68"/>
    <dbReference type="OrthoDB" id="415825at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000246">
        <property type="term" value="F:Delta24(24-1) sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040165">
        <property type="entry name" value="Diminuto-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10801">
        <property type="entry name" value="24-DEHYDROCHOLESTEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10801:SF0">
        <property type="entry name" value="DELTA(24)-STEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="414" mass="47474" checksum="DFDC8BDCC7796E4E" modified="2023-11-08" version="1">MELPNITVGGGFAGTSGESSSYKFGLFDRTITGIEIILGNGEVVWASANEHRDLFFTAAGSCGSLGVITLLEMELVEAKKYVDLQYIPVNSVQQAVEQLRLYENDPGVDYMDGILYSMTNGVIMIGRLTDEIMPGRLQRFDGAADPWFYMHAEDVLNKSRIENVNYKESIPIQSYLFRYDRGVFWSGLRAFKYFITPFNRITRYLLDPFMYSRTMVHALHRSGLSARAIIQDYGVPYDTTEEFIKWTDERTGIWPLWLCPVKSAPRDERSFSQGNNIKDEILLDVGIWDMGPDDPHAFIKLNRDFEAKVTELGGMKCLYAHAYYTEQEFWDIYDEKKYKELRRKYHAESLPSVYDKVKVDLQGVAGETTMAKKKEAWGEWAYGHFWRTWPFGGLYGVASATKGLLIQSDFLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH30</accession>
    <name>A0A9W8YH30_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002477</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH30"/>
    <dbReference type="OrthoDB" id="10497244at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="205" mass="22883" checksum="9F5E2A050ABEAE1C" modified="2023-11-08" version="1">MVAQKNWRDNYPNYDKAMESIKNGGPPLFKPGDIPSDVQQAEKWSPPKPASYATFTPQSDSAPSDPPKGAPKSLQIVYETFVSEGAESWQKWHFFEGVPRQAVDTCSDKVLFSVDNAPPPNGYDPPWINVKNHSFRSSGYAEDCRFDGLDGKGPSGDVGWLYCPERPAIMCVEDAKKSGGKDAWKNCDKGLWTRDRIPVAYCDWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHK3</accession>
    <name>A0A9W8YHK3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Rho GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RHO2</name>
        <name evidence="10" type="ORF">N0V83_000997</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHK3"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000573">
        <property type="entry name" value="RHO family GTPase Rho2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4378164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="200" mass="22298" checksum="6B27FF9547DE557E" modified="2023-11-08" version="1">MAQQNGEPVMRRKLVIIGDGACGKTSLLSVFTLGYFPTRYVPTVFENYVTDCRVDGKSVQLALWDTAGQEDYERLRPLAYSKAHVILIGFSVDSPDSLENVKHKWAEEARERCAGIPIILVGLKKDLREDPLAQEEMRKKSLRFTTTKQGSDMKEMIGARKYLECSSLTGDGVDDVFEAATRAALLSVDKREKTGCCIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHL5</accession>
    <name>A0A9W8YHL5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1765-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000696</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHL5"/>
    <dbReference type="OrthoDB" id="296767at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013887">
        <property type="entry name" value="UPF0592"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37988">
        <property type="entry name" value="UPF0592 MEMBRANE PROTEIN C7D4.03C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37988:SF1">
        <property type="entry name" value="UPF0592 MEMBRANE PROTEIN C7D4.03C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08578">
        <property type="entry name" value="DUF1765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="931"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="975"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="998"/>
            <end position="1013"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1142" mass="127064" checksum="1135E485279C2157" modified="2023-11-08" version="1">MPVAVASAPRGLANAFRGRNRAISEVGPPPPPPREPERAQLPKAASYTYFPRVKDLDQGPPVLELTGGTFDFDLHNGKHGERTSESSSAASSPASEEAPEEAVQMPQLRPSNSRRSSRFLSFPSRSRDPSAESKTDRSRSETVRRTESPGSSPARSLSKLRRKSWIVPQQQQQKAGSEPAKDKGRHKKEDSVKKQAASDATKRKTPASTVSIPDESEARDTVPDEPPLIPLSKKNKRLSGLFNASANAPAVPAVPKSFSTEKLPLYPQTHTPLSPTHLVPPLPRNTSNEKLKGVKTEPRKKDELWTVFRTLDADLRKHDSPVSQFLPTLTEDRYQSKSSALKANVVRTTLLPFLRQYASHPSNHKLRAEDLDRRANILNGWWTALLELVAGRNNQSISGTDRPAILEAMVGIMERPEWRQPPSGFAALADRAETSSTATTVSNASSSSDFLSESVYHNVRNIFTQNLLSQMAFVVEKMSLRNAAASLVTFCGKTCAYAFCFCPGIADILVRLWNPSLDMMKRVMQESGVSKMDRLDAVSDRVVASFPPTLQSLKFHSLPKLMRQLRKPASPPLAAANLDWYGLWMKRWSGAESDLFYVFVKHYHILVTDFLPVEPSNAERVCVPGLVMVHAQVLVNLDATINRHAAPPQTDDQGTVVAPITFDDVLGDADASASAVAIPPANATRLMQENRLIMLIRDFLSERNAHLSTARRVFAESFGRLLQAQARKISIYDSNACYTLCDFLEEAFAIMVRYEHNDSDHHSIMDWPFWLSVCKSMTASQNTATEIKLYAFLYTTWSTLCLSEQRKASLCLDFLLEEEFFASRFNHWCPMVRSYFMRLLCWRVARFDGEETETEVAIKQALSDRLRSVWSHYLWLHEDAESKQLVLPSTQACNPAPGRRFLIIRNDNPVVANSGPFLSFDGVVQNTHQRKFSPTANSIIEPEERPTSAMSTDSQDFPDEDVGKGKWNILRSLIGVSSKQNTKSKGSSPSAKDKENANKTTNVAGGKTSNDTPSPDRPTASQAPAPAPAPPTHRSYSFRFSLEWVDKRFGTYQNMRLQPPRLPVPAQTLLLQNGINIEAVIPSQPLGAAATSSTYAGRALAEWTFVSHECQNFFDRRKNEGVPSNRQVETPTLNVEAFRRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YI98</accession>
    <name>A0A9W8YI98_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YI98"/>
    <dbReference type="OrthoDB" id="206452at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.40">
        <property type="entry name" value="YbiA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012816">
        <property type="entry name" value="NADAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037238">
        <property type="entry name" value="YbiA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08719">
        <property type="entry name" value="NADAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143990">
        <property type="entry name" value="YbiA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADAR" evidence="1">
        <location>
            <begin position="6"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4378196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="103" mass="11547" checksum="D4C0660427687531" modified="2023-11-08" version="1">MQEALMIATRANMRKFFDGKKAEDLQNRLVAIGPRELVFAAPSDKLFGIGFGATDAEQASRDQWGQNLFGESLDTVRKKIAQMSDPEFPGVFDKFGGKKNVYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIV7</accession>
    <name>A0A9W8YIV7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001696</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIV7"/>
    <dbReference type="OrthoDB" id="3940153at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="526"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="535"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="664" mass="73393" checksum="844BD7649F2AC79B" modified="2023-11-08" version="1">MSSVRQTYTALPPKSRPEEVQPYQYISQFGIGTEPWHSVKESDATSTAASCLDFNVGQWLPTSPQISVFPDGIVHEGRQKLQREINSSIEVEELPRAARIVFTNLYTKLGSETFSQLCDRTFTRWEGFVQHLRDDQSDCYAVYAKILEDLYAVLHAIDSGAIHAAKYGSTEQSLTYRPLHDVEGGSREPGSLSLGLSANPVSPASQGSKLVGHRASRVNGSSRNLKVATGNKSKRAGSRAVDCPEYKHHIMHGTHPPSCGGCSEVVMSQVRSHLKRVDHRDFPSGVWQCPRCKKDFVDQSIYDLHHRDDTCRHQPQIRKDIELPWARLYLSRYPTATRIPIPYCDQNGWLPDSILAQCRLSLANSSASFLGQHDSGPSGMQGHIPANVTSIDGASYNAGLSFMLQDLLDPSSSHAHEQATVLARRVSGLNTIDPQTFTQGPATADVERFSRALHNASRDYFRMLAMAPQYLTDDQLRQTANEAAAVVHQIRNYQEQSQQNQIAALPYPSSAQEQPLGRHVNPAYVPPWHNPPSPSASNEQYAAAGMSTQPSSQGFDTASSQYRLSMQTTSPSSAYNSSNQMLLSPFATPQDYRRTSVSSQAATGAPHHYRASSATRSLHDECLQARNRVNPSFTTIDPSLLTTPHSDSGHEVDYLRRQFFPGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJE4</accession>
    <name>A0A9W8YJE4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="14">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">CNA1</name>
        <name evidence="17" type="ORF">N0V83_000437</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PPP phosphatase family. PP-2B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="14"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJE4"/>
    <dbReference type="OrthoDB" id="5593063at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033192">
        <property type="term" value="F:calmodulin-dependent protein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097720">
        <property type="term" value="P:calcineurin-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07416">
        <property type="entry name" value="MPP_PP2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000002">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041751">
        <property type="entry name" value="MPP_PP2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043360">
        <property type="entry name" value="PP2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45673">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2B CATALYTIC SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="6 14 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="16">
        <location>
            <begin position="181"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="412"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="508"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="508"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4377610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="551" mass="63383" checksum="3B1B4A2C20782868" modified="2023-11-08" version="1">MENSKPEIDQSQYIDNAMRAVREKKPRPEIDFTLHTMEDNTQVSTMERVCKDVQAPAFHPPTDEQFFSPQDRTKPNIQFLKQHFYREGRLTEQQALWIIKKGTEILKAEPNMLEMDAPITVCGDVHGQYYDLMKLFEVGGDPAETRYLFLGDYVDRGYFSIECVLYLWSLKIWYPNTLWLLRGNHECRHLTDYFTFKLECKHKYSEAVYEACMDSFCALPLAAVMNKQFLCIHGGLSPELHTLEDLKSIDRFREPPTHGLMCDILWADPLEEFGQEKTNDFFVHNHVRGCSYFFSYPAACAFLEKNNLLSIIRAHEAQDAGYRMYRKTRTTGFPSVMTIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCTPHPYWLPNFMDVFTWSLPFVGEKITDMLIAILNTCSKEELDEETPSSLASGPSSPPLPSLDPESTEFKRRAIKNKILAIGRLSRVFQVLREESERVTELKTASGGRLPAGTLMLGAEGIKQAIHSFEDARKVDLQNERLPPSHEEVRKSQEVSREQALEKATKEADNDQGLATVARRISM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CGX3</accession>
    <name>A0A9W9CGX3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Inositolphosphotransferase Aur1/Ipt1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_010158</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CGX3"/>
    <dbReference type="OrthoDB" id="2566866at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd03386">
        <property type="entry name" value="PAP2_Aur1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026841">
        <property type="entry name" value="Aur1/Ipt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052185">
        <property type="entry name" value="IPC_Synthase-Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310:SF10">
        <property type="entry name" value="INOSITOLPHOSPHOTRANSFERASE AUR1_IPT1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14378">
        <property type="entry name" value="PAP2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Inositolphosphotransferase Aur1/Ipt1" evidence="6">
        <location>
            <begin position="234"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4363040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="421" mass="48698" checksum="6CC5A0DDB99FA4A0" modified="2023-11-08" version="1">MPNFTVPNGDQWNSKPAWKLPGWCEPVMVASILFFAMFMTRKRDYKIFDRTSSGAGYLDGTSNGSTDYLLPEDTDSDNEYDDISTAKNLPKVRRICCWKVTTPNTSRFRNNIHSRVLQRFPFLIEMFYWIINYAFYRMTSVLSSKLFAGRGIWEVAQEHGIAVLEAEEYGMLRFLFPVRERDVQQWFMQGHQDALTILNKAYALIHIPGTVGSVTSSHGICMSTDIDSFICWYYYVAPSFNTFAIARRTLTLTNFCAFVTFVIYPCMPPRLLPQEYGFLDTVRHDDAQSVWMSGKFVNQLAAMPSMHFGYSFCIGMAMIYHSGLFRRTLERGEVRKNIFWKIFYLIVGVGYPSFILTTIVATANHYIMDAMVATFFVFFSFFCNKVFYLFIPLEDLLLFIIRADKPIPTTGDRFHERGGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CGZ7</accession>
    <name>A0A9W9CGZ7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010235</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CGZ7"/>
    <dbReference type="OrthoDB" id="306876at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="359"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="53"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="247"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4363115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="407" mass="44095" checksum="05CB10A110926086" modified="2023-11-08" version="1">MSYGATASTASEGHPENDALPPTTGTGRGAEDEQLAIWRRTQQRLKSFFDRNFGLFLVFLAQTCGSIMNTAAKLLANDPTIKFHALQIIFIRMFCTTILGTLYMCYHRVPDFPLGQRGVRGLLVLRGTAGFIGIFGLYYSLSWLNFADATVITFIIPTMTAFVCFVWLREPFTINEALAGLIAFVGVLFVARPPWLFTSPPLDPISGEPEPSHSLMFVASSEAPVGGKAPTVPTTPQQRALVILVAVVGTFGAAIAYATIRVIGKRSHSIISVNYFAAIATLGSTIIIAVHPDLHFVLPKNAAQWGLVAIIGVTGFLLQFLLTEGLQREKGGRATNLTYLQLVFALVVERVVWGTTPPLESLAGALLIISAAVWVSLQKNSGGAAQKKVEVVNEESSLLGRTEERER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHP5</accession>
    <name>A0A9W9CHP5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_010098</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHP5"/>
    <dbReference type="OrthoDB" id="3257095at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF1">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="388"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="420"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="483"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="514"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4362981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="551" mass="60274" checksum="4B2E31A204B11CFA" modified="2023-11-08" version="1">MEKRIDLQTFDDDLRARNKGSISESKGQDFDKDRYELARVGKQQVLKRRFGLVSMTGLSCGLMCTWESLLVIFSIGFTNGGPAGLIYGFIIIWLGNFSVFICIGELASAVPTAGGQYHWVSLLAPRSNKKFFSYVTGWLTVIGWIAALTSVCFFVADLVLQLVSLNNVDGSFVREGWQGTLLLWATLLLCVFINVFISGALPAIEVVVLIVHVLGFFGILVPLVYLTPTHNSAKQIFTTFHNTGNWSTQALAFFVGLQGNALAFVGTDSVVHMSEEVKNASTDVPRSMLFSLVINGSLALGMLFAVLFSADDIQQLLDEDAPTSPFIRIFRHAVGSEAGATVMVSIIILLEFCSAMGCLAAASRMTWSFARDRGLPFSRALSMIDKRTTIPVVAIIVVTVFAALLALINIGNSAAFNGTISLVLEGFYLSYVLALGLLLWRRIRGDLDNPDSSLTIFNSGHVDEAYDRSLTWGPWRLKGAFGIANNVVAICYLLLIIFFSFWPNSVNPDLVHMNWAGVVTGSVALFSVAYYLLFAKKSYTGPIVEVDPHVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHV8</accession>
    <name>A0A9W9CHV8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_010281</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHV8"/>
    <dbReference type="OrthoDB" id="3363151at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043007">
        <property type="term" value="P:maintenance of rDNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007096">
        <property type="term" value="P:regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018819">
        <property type="entry name" value="Nur1/Mug154"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28293">
        <property type="entry name" value="NUCLEAR RIM PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28293:SF1">
        <property type="entry name" value="NUCLEAR RIM PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10332">
        <property type="entry name" value="DUF2418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="325"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="325"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="345"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="431"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4363161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="497" mass="54628" checksum="964B33D2E4658737" modified="2023-11-08" version="1">MPRLVRRAPLSERIKAYTDPSDWLIWISEELNSSDWDEFASSYALYIGFSANILFIIAQANSGASTSYSDDDGVFTAVSGPGWIKWMMNLLVLVLGGASLTNAWLVWGKRRYYRLFEQSVEVAPQTPSAKRVRVDSSPAGASPLSFIKSVMSNSAAARAHPDAERDVWEIAVWDPNPLCLELFCLFSPLHVVLYYLNLPVEPLDPRPSVKVTTTIFIGAILSSHLWWLRKSFSQQAKDNAIISRGVLHEYDNKFVHPALQKPCRDVGIQTISRKRNRDSSVGVQGSSDDLASEVVTYTPTTIINRTFRTNPNANYAAQYDPDNLSSTSVMRSSQQTPSFRPGYHTTSSYTNTSTATGADFSSPIRPSNTPNPFRQAPPIPRTSDGGYLGVKQHAASPLRKSSSTSFIRDSDRSSGSGLTGPRFDAYGQPVDRSDARGRESLGGAGERRQASPSKREGSPLKRMSMPNGERPLGQAERLGRSSGYGGLGVGRRESGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHW3</accession>
    <name>A0A9W9CHW3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcriptional activator of fatty acid utilization</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CTF1</name>
        <name evidence="8" type="ORF">N0V83_010296</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHW3"/>
    <dbReference type="OrthoDB" id="25391at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052073">
        <property type="entry name" value="Amide_Lactam_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47171">
        <property type="entry name" value="FARA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47171:SF3">
        <property type="entry name" value="FARA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="7">
        <location>
            <begin position="245"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="540"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="703"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="607"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="707"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="751"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="763"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4363176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="811" mass="91231" checksum="8319C681EFBC15E3" modified="2023-11-08" version="1">MRGNDRGDSDDRSPASPSVEPRDRTAVYNSEDGTPSTTVSSAYAAQQATHNGTYVQFMKPKFARAPIKEAGRVAYLGESSNLSLLVHDRYGTTDVVHYPLPENVRGAKARVNEMDNMEIEILHQRGAFLLPPRALCDELVEAFFKWVAPVVPVINRSRFMRQYRDPKNPPSLLLLQAVLLAGSRVCTNAQLMDASGSTTPAAMTFYKRAKALYDANFEDDRVTIVQALILMGWYWEGPEDVTKNVFYWTRVAIVVAQGSGMHRSVEGSQLSRSDKRLWKRIWWTLYSRDRSVAVALGRPVAIDPEDSDVEMVSEDDFIDDETDHAAEHPPDPVHVQFFINYVKLSEIMGLVLSQQYSVASKHRRGNAIDLTHSDMALADWLQHCPPEVQWDRSHHHFWAALLHSNYYTTLCLLHRAHMPPAGSPKANNLDGFGEEHAYPSRNIAYQAAAMITSIIENLRAHDEIRYTPAFIVYSLFSALIMHVYQMKSSNKSIVSATEQRLQICLDALKEVSKVWLVAKMVHTLFESILGNKHLEERLQKAAGRNHKKNKASSGPPPPPKPAQEAPKRKFDDMDLGFPATNGPPAPQVSYERSRPQTPAVTPSRDLPPQQQMPQMTTGSPQMNRQNHDAFMGPSRSGTRPTTPFNPSYSYPGTPPDLFLVTRDTPNISQEVWQNFQPDQLFPADTQVNFPQISPMQNHSLVDPALSQPQNMPLHQQTQQQLPGNPQQMQTPQPNMGNPAGFEHNNPQAWAQQMEMMSQNQQQHSAQDDNWSNSSGGRHNIVPMSLNVDDWFNFFGINGETDMSHLFQQGPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIB2</accession>
    <name>A0A9W9CIB2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Polyadenylation factor subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PFS2</name>
        <name evidence="6" type="ORF">N0V83_009926</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for 3'-end cleavage and polyadenylation of pre-mRNAs. Also involved in chromosome segregation where it has a role in chromosome attachment to the mitotic spindle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIB2"/>
    <dbReference type="OrthoDB" id="16717at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002010">
        <property type="entry name" value="WD40 repeat-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045245">
        <property type="entry name" value="Pfs2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836:SF0">
        <property type="entry name" value="PRE-MRNA 3' END PROCESSING PROTEIN WDR33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="82"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="124"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="165"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="207"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="249"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="362"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="415"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="432"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="455"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="496"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="545"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="623"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="633"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00026154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4363630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="673" mass="72896" checksum="B90DE00221C8684E" modified="2023-11-08" version="1">MEREYHQRDFDGPPRPRPKRAVTDYGATTVQWMRNRRPRYKNMAMPEVERPSASYIVDMEPPAARLASAAESIPAKALHSSLNKVKHPINVVKWTPEGRRLLTGSTSGEFTLWNGMGFNFETIMQAHEAAIRAVEYTSTDDWLLSADQTGVVKYWQTNFNNVKEIQAHTEAVRGLAIAPTDSKFVTCADDTTLKIWDFASSTEESTLTGHGWEVKSVDWHPHKGLLVSGSKDHQVKFWDPRTGRCLTTLHGHKNPISKTMFEPIQGNMLATCARDHTARIFDLRMMRDVLLLKGHEKDIITMAWHPMHKNLLSTGGVDGSIHHYLLDEQNPPAGVAPSISPYDAADSQNAPAQTIYPAHSMQYAHDFTVWTMDWHPLGHILASGSNDRVTRFWTRPRPGDTTYIKDRYHIGQAAAEAQGTYDRGQGRRQKQEEEEQEAEDEAEGLVDQKLPSRNPGAPSFLPPGLSLPGLTAAPDGTSSALPGIGGAPPQHAGLPFHPPQPGSNASAPPPLPPAFAGMDPSRLAQLIQSGALPPPPFPHAQGGGGPPPLPNGSTGAPPPPFPHPPPNFPFPGGAPPPGMPPLPPGMPPPGFPGFPGFPPPPPGGMPPGALAGPPLGWTPPAPQQQVQQQYAGEQGGPVGGGGAGMGVRKRAPLPDQQDALKMEMNQGRYRKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CII0</accession>
    <name>A0A9W9CII0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009509</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CII0"/>
    <dbReference type="OrthoDB" id="2947043at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4364054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="14979" checksum="996F8C099AFA68E4" modified="2023-11-08" version="1">MSEYTSTREGFQRAMKMSLIGPPEEARQYAEATATPNFYHIFNGERSEYDAWLQGIVEWRGKITDYDPVVHEFLRDGDQLAARMTGVAKVDGAETSYECFFFAKIEMESGKIESLTERAVWGPVGKAPEHGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIY6</accession>
    <name>A0A9W9CIY6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_009293</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIY6"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000006">
        <property type="entry name" value="General amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF6">
        <property type="entry name" value="AMINO ACID TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="329"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="374"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="403"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="448"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="480"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="8">
        <location>
            <begin position="43"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4364696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="552" mass="61271" checksum="226D66BFE4BE98D4" modified="2023-11-08" version="1">MNTEEKKYSGEFVREAYPNEDIQHGEVHDNSDDLQRHLGNRQIQLIAIGGSIGTALFVSIGGALNKGGPLGLFLAYTGYSCIIALVNNAMAEMASFMPISGGFIRMAGHWVDEALGFMAGWNFFLYEALLIPFEITALNIVLRFWRDDIPVAAVCAAVIVLYGACNVLAVKAYGEAEFWLSGGKVFLILILYCFTFVTMVGGNPQKDAYGFRHWRDPGPLNDHGDGSLGRFEGFLGSLWAASFCIVGPEYISMVSGEARRPRVYIKNAFKTVYFRFGAFFILGALCVGIVLAYNDPELVSVLEAGESSAAASPYVIAMKNLNITGLSHLVNALLITSIFSAGNTYTYCATRSLYSLAIEGRAPKFLRKCTRNGVPIYCFLIVMIFPFLSFLQLSDNSAKVLTWLTNIITAGGVINYIVMCGTYLAFYKACKVQGLDRKTLPYCGWFQPYGTWFALCFEIVVVFVYGYSTFLPGAFTLESFFTYYTMVGVAPILFVFWKLFKRTKWLKPQEVDLVWDAPVIDAYEASFVSPPQTEMLQLVGLKRNVPVDKRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJD2</accession>
    <name>A0A9W9CJD2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008964</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJD2"/>
    <dbReference type="OrthoDB" id="2016523at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22189">
        <property type="entry name" value="PGAP4-like_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029675">
        <property type="entry name" value="PGAP4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31410:SF1">
        <property type="entry name" value="POST-GPI ATTACHMENT TO PROTEINS FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31410">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="392" mass="45083" checksum="94E2334FCB555AF4" modified="2023-11-08" version="1">MPINDPTSIFFSPQKGYTPRYSALRHQQAEAFITTYNSTLDRNVVKADEEQKKLCIGIPSIGRKGARYMRETVGSLLDGLTPQERREIYFIVFIPHADPTAHPAFNETWLHGLTDQVLTYGSDIDDDQMQYIRDMEEEGGNFEEKGLYDYSYLLSECVKQHTPYIAIFEDDTLAMDGWFHRTMAAIKDAEKQSELRQAKHFLYLRLFYTEEYMGWNSEFWKTYLCNSVCMAAVLAALLLLLRTSKLAGMLSRSIGGTRAIITIYAILATCILMFFALGRLTVLPIQNGVHEMSRFGCCSQALVFPREKAIELVDYFEEAHIGFVDVLIEKFADEREELRFAITPSVVQHIGKTSSKWDSAGAIYKEKIWSFGFEKYDWETLSKEHKKVRSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJH0</accession>
    <name>A0A9W9CJH0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosomal protein S2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008252</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJH0"/>
    <dbReference type="OrthoDB" id="2320368at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000061">
        <property type="entry name" value="40S ribosomal protein S2, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_B">
        <property type="entry name" value="Ribosomal_uS2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005706">
        <property type="entry name" value="Ribosomal_uS2_bac/mit/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01011">
        <property type="entry name" value="rpsB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S2 PROKARYOTIC AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="417"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="132"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="435"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="458"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="483" mass="52755" checksum="7A8448D8A58941A7" modified="2023-11-08" version="1">MFYRGICPGKGDGWDQLAATAGSEPQATQPTRLSMMTGQLQGPTRAFKGCSAPATPAEPWAAHDLHPVTLRTVSPAYGLALVAAWRPRTFAQCSSRPLRRFLSTHADSLNASSENSAKELQSLEAQLNSHLQQSAPPADAQPQQTSSPVTDNVAEQYYLYKQQAKQMDKLGSDISPHYQPHALLNNPPSPADVTLELLLASEAHQGHVTSLWNPANARYIHGIRQGVHIISLEVTAAHLRRAAKVVQEVSRRGGIVLFVGTRDGQDRAVARAAELAKGYHLFERWIPGSITNGQQILGRCQTKVVNELDEDVLGFEEQLYERPVLRPDLVVCLNPLENYVLLHECALNNIPTIGVIDTNADPTWVTYPIPANDDSLRCIQVIAGVLGRAGEAGQKQRLNAAARGEITYRPARGLVLGEESEEADSPEAAASTEKVGAEESRLERIMPLEEGLEDSNELAEKLDEVNNHESHRTREGRAIKISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJL2</accession>
    <name>A0A9W9CJL2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ABC1 atypical kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_007615</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJL2"/>
    <dbReference type="OrthoDB" id="201153at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13970">
        <property type="entry name" value="ABC1_ADCK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034646">
        <property type="entry name" value="ADCK3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051409">
        <property type="entry name" value="Atypical_kinase_ADCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851:SF3">
        <property type="entry name" value="COENZYME Q8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="5">
        <location>
            <begin position="70"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4367085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="422" mass="46616" checksum="378A90BA8A1204A0" modified="2023-11-08" version="1">MALGAVGESLRRVTGSAASNTGSLMLSPGNLEILVAKLSRMRGAALKLGQMISFQDIKMLPPAIHEVLSRVQDSADYMPASQRNKVLSSNLGENWRDLFDSFEDVPVAAASIGQVHKAVLKSTGQAVAVKVQYPGVAGSIDSDLSNLSILLTASRILPKGLFLDKTIANARTELGWECNYTREAECQTRFRDLLADDPDVFTVPKVFLEASGPEVLTAEFMDGVGVTKLKNLTQEQRDWIGTQILRLCLREIVEFKFMQTDPNWTNFLYNAQKQKIELLDFGASRDYPDEFVEPYVKVLIAASKGDRESIRDLSLELGYLTGSESPAMLNAHIDSVLTLAEPFSGSGPEIYDFRDQTITDRVRGFIPVMVNERLAPPPEETYSLHRKLSGAFLLCARLGSRVPCRDLFAKSVETWEGKATMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJM2</accession>
    <name>A0A9W9CJM2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Structural maintenance of chromosomes protein 6</fullName>
            <ecNumber evidence="15">3.6.4.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">smc6</name>
        <name evidence="15" type="ORF">N0V83_008403</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="15"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJM2"/>
    <dbReference type="OrthoDB" id="10072614at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES 5,6 SMC5, SMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306:SF6">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecF/RecN/SMC N-terminal" evidence="14">
        <location>
            <begin position="99"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="266"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="481"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="716"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="924"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4365782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1144" mass="130004" checksum="6F7CE7A4599555D1" modified="2023-11-08" version="1">MAAVISAKRPRAFTEDLGSIGGAGPNSRNKRARPSTNDSRGQSSSADEQSNASSDSDASDNEREDTQEYEQMAWATQQVQRDFRATANKQNVATDSGIIEEIQCINFMCHEHLTVTLGPLINFIIGHNGSGKSAVLTALTICLGGKATATNRAQNLKSLIKEGKDHATVTVRIKNQGPLAYKPSQYGDSIIVERHFTKSGTSGFKLKDRNSRVVSTKKAELEDILDAFSMQIDNPMNVLTQDMARQFLNNSTPKDKYKFFLQGTQLENLNRDYQQIEQSLELMNAKAEVKETDLGMLRKRMEDLAGKARRAENLERMRAKETVIAHQAAWARVEQEEADLVKAEAEVERVDLLIQKRTGIVDEISESYERADQAHQAAQQDVTDITTELEPAKQLEKEAKQHFQEMRQKIITLKSDERKVGGDVSKKEQEVKKHESEIEQLRQRQAEADNGLHAEKVQELEVAKAEFERSKEVYTNHDTSLPALQEELKAARTEHTIAEQKAAKAREDEKRIRNNVSNLREGQRNWLDAYPNPGGLEKLLRAIQSDRRFREQPIGPMGRHVKLGRPEWGYILEKQFGSVLNGFVVTSKADQTTLTDLMRKNNWNSPVFIGNRNPIDTTQNEPDSDMLTWMRALKIEDHLVRNQFIINQAIEQTVLIEDRTEGSRFMHARGPRTRNVKMCFTFADGDKRRGRVINFTSNGGINDSPIDEFQGQLRMQADKEAQIREEQARLEQVRQEVRELEQASRDVQNRVNTCRAQQQNHERQRKILQVNFQKAQDKVDRLEGELSEATPDAAAIGVLEDALADAQGELQRAEGLFEDMVLQRDKLDGDARASKTTMDDAQKAVQNLEFKLQKAHATVRRLQGQREDELKKKNHAIAQVAAAQENRDIWAEEMRKAQIEVDTATEDAKTICPQRVIVPDGKSSEELLTTLERLMATRRETEKELGGSQDELLRLANDAKKTHKDAMQEFENIKSLRNHLVNTLNNRRNRWKQFRSGISVRARVTFNYLLSERKFRGTLSIDHNKSLLDIHVQPDITERSGDGRQTKTLSGGEKSYSTVCLLLSLWDAMGSPIRCLDEFDVFMDSVNRERSMNMIIQAARRSIGRQFIFITPQSMNNVKQTSDVKIIKMSDPERGQTALNVRQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJW6</accession>
    <name>A0A9W9CJW6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyrosinase copper-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_007966</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJW6"/>
    <dbReference type="OrthoDB" id="6132182at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1280.10">
        <property type="entry name" value="Di-copper center containing domain from catechol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008922">
        <property type="entry name" value="Di-copper_centre_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050316">
        <property type="entry name" value="Tyrosinase/Hemocyanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002227">
        <property type="entry name" value="Tyrosinase_Cu-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474:SF125">
        <property type="entry name" value="N-ACETYL-6-HYDROXYTRYPTOPHAN OXIDASE IVOB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474">
        <property type="entry name" value="TYROSINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00264">
        <property type="entry name" value="Tyrosinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00092">
        <property type="entry name" value="TYROSINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48056">
        <property type="entry name" value="Di-copper centre-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040987986" description="Tyrosinase copper-binding domain-containing protein" evidence="3">
        <location>
            <begin position="23"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosinase copper-binding" evidence="4">
        <location>
            <begin position="108"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4366330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="400" mass="44171" checksum="6EE3D8B08A641885" modified="2023-11-08" version="1">MAGNTVVNAVLASLCLFSGASAWPFKTRQTALTLDNVQEQALANAYKVLDGTLADGLTRSTGCSKDTVAVRKEFGDLTKDERKEYTRAVKCILEKPSKLPAGKYPGAKSRYDDFVVVHMNMTPSVHATANFMHWHRYYIWAYETALRTECDYKGWQPYWNWAKYADLVNSPIFNGDEWSMGGNGDPIGAHAGTYIGSQMLPGGPGGGCVTKGPFANLTIHLGPLMPTMDPKLGIKSNPRTDGFGDNPRCLRRDVSNFFTASYMRPQDLLDQITAAPTIGKFQDSLQDAGAKKPALHVGGHFSIWGDPGGDVYVSPAEPTFWLHHGQLDRHWWMWANYQEKELKSRTSMYEGGTNWMNPNSAKGKPTDNQWLDVVAPAGKNGLPSNQFFSTTAGPFCYVYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKD4</accession>
    <name>A0A9W9CKD4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007616</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKD4"/>
    <dbReference type="OrthoDB" id="5419904at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="254"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="414" mass="45363" checksum="759EDB871DB37D9B" modified="2023-11-08" version="1">MAGRRLVDVAKLFNASKSVAQKHVALRSNQLDVYNKTSTLAKAVKNQTDRVTLTAAAAIALSQRFSEEAPAYAKAAAERATGTHNEHVPRSDTVKRDGPVQGVKDGIEQDHHYDRSGQNTTAEPPREEELHVEQKQAQRRPLPDGTIPTADVTIEQEGRGHDTFSKRPVPEAPKEPLAEDQGEQVQHENEGLKPVESTESTIPLPGQPKGTSTQSSEAIPSHANDLQQAPISPQVQQLQEDHDRDVFYARSVESQPAPSSQPRSQIPSHTETKQGADKHVDDGQLNQDVYYSARKTGQEQAQKVTQEEDISEGINTDVFHSKRVARMLGSDPLSRKDHLERRSAGRHPLDDRPVPQGNATSDTRAPEQAQNAYSQPIAESNSTTKEMEDFASQLAQDVQSNAAAAEVPPTYSFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKG6</accession>
    <name>A0A9W9CKG6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CN hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007617</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKG6"/>
    <dbReference type="OrthoDB" id="201515at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008418">
        <property type="term" value="F:protein-N-terminal asparagine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070773">
        <property type="term" value="F:protein-N-terminal glutamine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07566">
        <property type="entry name" value="ScNTA1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039703">
        <property type="entry name" value="Nta1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11750">
        <property type="entry name" value="PROTEIN N-TERMINAL AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11750:SF26">
        <property type="entry name" value="PROTEIN N-TERMINAL AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="305" mass="33806" checksum="B7FEC1BA96B5822E" modified="2023-11-08" version="1">MKVACLQFAPEVGNVQENIRRADNILQNTRIPTDLDWLVLPELAFSGYNFHSLEEIRPYLEPTTSGVSTQWAIQVALHYTCHVTIGYPEITTPSTNPSDPQTQYNSTVTISPQGTILHNYRKSFLYYTDETWASEGPASNSHKLSNAESSDAPFFAGELGSLGKVTLGICMDINPYRFTSPWAAYEFASLALSSSTPLICISMAWLCHLTPTELMENPTQPDVATVAYWVERFQPLVEAKGDKPVYVILANRCGMEKGVCYAGSSTVLRIEKGAVSLFETAGKSEEKCLVVDLTERPKFQVRSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKI9</accession>
    <name>A0A9W9CKI9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006712</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKI9"/>
    <dbReference type="OrthoDB" id="5228334at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4368355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="238" mass="25333" checksum="4524FB8A94EDE477" modified="2023-11-08" version="1">MSITSIKCTKVSRYGDTTDFVRSESIEGVATKYYEIDSSTTKWIGVNTEYLTDIVETTTVNNVQTTKTTSVALIAATATAASNGLEDGAVTIVMSPALTNILDQLAADAATICLETRKRQTCDVRQEFANLVNTNLQTGGSLHEFDFDFLVPTIAAEDVAAIVSGVTNALPILGVFVMSYLIFAESGAAKIPPVMNIPPSLTVAPTATATASGCAYPTATGENRVCFVFSHQWPRKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKV1</accession>
    <name>A0A9W9CKV1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006036</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKV1"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF62">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="2">
        <location>
            <begin position="50"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="403" mass="46285" checksum="AC921102B846CFD7" modified="2023-11-08" version="1">MPAGTYWKSSRVKKGEVERPWLDKKDPRDKWNTIFPVAGLVLGLIVVGILIWDAIRSTPMHKYCEVLNENFTSWNSSVWTYEVEVGGYGNGQFEMTTNTDENVFIKDGVLIIKPTLQDEKFIENNHTIDLRGQGCTGSKWTDCVTTTNTTNSTIINPVKSGRINTKLGASIKYGRIEVVAKLPKGDWMWPAIWMLPKDSVYGDWPRSGEIDIMESRGNNKSYAQGGNNVASSTLHFGPDPDNNGWWRNQRKRQAFHTTYAEGFNTFGIEWSEKYIFTYINSRLQQVLFNRFKRPFWDIGEFPLASSNGTRLINPWKDTGSNASPFDQDFYLVLDVAVGGTNGWFEDGKSGKPWIDRSSLARKDFWDTRDEWYKTWRQGGQMEVRSVRMWQQEGYNGCDMNTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMT7</accession>
    <name>A0A9W9CMT7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6604 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005066</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMT7"/>
    <dbReference type="OrthoDB" id="5238236at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046539">
        <property type="entry name" value="DUF6604"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38795">
        <property type="entry name" value="DUF6604 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38795:SF1">
        <property type="entry name" value="DUF6604 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20253">
        <property type="entry name" value="DUF6604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6604" evidence="2">
        <location>
            <begin position="14"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="653"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="832" mass="95640" checksum="4B6072C841E0B557" modified="2023-11-08" version="1">MDLYARNELPETVKRYKAYTAQFQEWLILTAVQRNVERASQIWERAKQPKGKKTPNISIEQQARLVDDIAKTRGPLADTSGLRDLSDAIRSRKEITQYYAAIKSSDTGHTHFNGALERCRAILKGMLPSLYETQDVEDESSNFIVTALYNAGDDDDEVDAEGAEKTAEPEDRPDIDNSKKGSSRIPKTRSNNDPLTAEEYELQREYRVLVFLYELKRIRGIVCDMWTLYNKGEINTITAALVADLAQSHVQQNVVALVEELSLRPGGLAELVLKLYWKMLGDTSSEPGEGTTPQFSKDTLRHLFCIDGISSLNFYLRTADTKVEEDQLKTSQRPYQAVLRFFEAIRNRKVKLPIWDKFTEDMLLHGRESKDWLYFGWQIVVDVQKIVCVHSPILLSDITEQSLDTFKMMRDHVDYEDAMWKSGEKPDYLSSEEFKFSDVFLNPLESLLGWIQQLTSDEVPAADQKLPMHEFVRVHTTLAGLSMWHFNKVYQGTSIRKVQWFVTCLAHLYNAARQVGDLNIDWPDLHFIVQMHGTQRLFLGDPPTDSKDFLHRFWLATCTSSRFMASDHRNKGRYAPRTARETHHKRALMPHFPLEETIRSYYGPNPRDDRWMKRHAVFNYLHQRKEPPAAHVDGAEPPEDDAKVEELRDTFTRMAAKISPSSSSTSSPKKDRKGAPPRVPVPDFSAIDDTYADLFSRMKTELKSHQLHTNFDYLSFYRRAYTLIMYIRENVLLDGTMQIARSGEINQNPNPSNITLLTGLFRGLKANPKDRKKVGDDGDDGNTVAWEQLRRIGEVMEGLIEEEGGVELERAKLRTRCDWEGLKASYAKEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMX8</accession>
    <name>A0A9W9CMX8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3-dehydroquinate dehydratase</fullName>
            <ecNumber evidence="2">4.2.1.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_004362</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.10" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMX8"/>
    <dbReference type="OrthoDB" id="204377at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046279">
        <property type="term" value="P:3,4-dihydroxybenzoate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00502">
        <property type="entry name" value="DHQase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000135">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001381">
        <property type="entry name" value="DHquinase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050146">
        <property type="entry name" value="Type-I_3-dehydroquinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01093">
        <property type="entry name" value="aroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43699">
        <property type="entry name" value="3-DEHYDROQUINATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43699:SF1">
        <property type="entry name" value="3-DEHYDROQUINATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01487">
        <property type="entry name" value="DHquinase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0704">Schiff base</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4371146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="345" mass="37617" checksum="4F549DF132640E63" modified="2023-11-08" version="1">MPDIIFDNFAAKKQTFIIPLTFTDITAAKDVITRIGYGGDIWELRVDLLSPAPGPLTTATNLPSPDYVEAQIKALQRMSDLPILFTIRTKSQGGKFPDDAAKEALELMLLAVSCGIQYIDVEVEWPQSLLGEITEKKGSTKVVASYHSWTGNIRWTSEELQKRFESANKFGDIIKLSILSATLEDCFELGLFVRDYNTKFSKPLLAVGMGAHGQLSRITSPISLVTHPLIPFPSAPGQLSLAQVHQAQHLMGMLPRREFNVFGEKGESAKAAAVAAVFQQGFQELGLPHVCQLGGESGASASGFDVANLFAGGRVEDAFDKQFSAWTGRQAPSLVLSDAWKRSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMY2</accession>
    <name>A0A9W9CMY2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_004377</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMY2"/>
    <dbReference type="OrthoDB" id="7786253at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF116">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE (CYTOCHROME) (AFU_ORTHOLOGUE AFUA_7G02560)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4371161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="322" mass="34823" checksum="BBC7696413670FFF" modified="2023-11-08" version="1">MKEWVRSLTCVLADGSIVKTHNRPRKSAAGYDLTHLIIGSEGTLGLVTEAVLRLAPLPQNLHVGIATFSTFQRGVDIVVALQKAGHQLEALELADGPQMHAINASKLAKLSFPEKPTLFLKFAGPSLQLVNDQISVVKQLCEQKEALSVEITADQQRIDVLWGARKCIGHALVAMKKHPSDLFMHSDCAVPISNLAALVNGTQSLIQAANAAGNHDWFCANVGHIGDGNVHSSIICPEKDREAVEKVLMQVVKLALRLEGTVTGEHGVGLKLRDALEEEVGSVGVEIMRRVKRSLDERGILNPEKVFRLEGNSGDEKGVAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN18</accession>
    <name>A0A9W9CN18_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">UDP-galactose transporter Gms1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">gms1</name>
        <name evidence="7" type="ORF">N0V83_004483</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN18"/>
    <dbReference type="OrthoDB" id="408493at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015165">
        <property type="term" value="F:pyrimidine nucleotide-sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007271">
        <property type="entry name" value="Nuc_sug_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00803">
        <property type="entry name" value="nst"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10231">
        <property type="entry name" value="NUCLEOTIDE-SUGAR TRANSMEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04142">
        <property type="entry name" value="Nuc_sug_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005799">
        <property type="entry name" value="UDP-gal_transpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="350"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="351"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4371266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="374" mass="41113" checksum="FD2F49BD299C469D" modified="2023-11-08" version="1">MPLALYEMSRNASSHTTATNLFQNLTIAIFANESWKLAIPAVLYTIQNNLQYLAVSNLDAATFQVTYQLKILTTAIFSVLMLGRSLSPRKWISLLLLIIGVSIIQVPQATMQSAGVETLKEGDAAAQLSRRSASYEGIEEDHAAHVPHMNRQIGLVAVLVACALSGLAGVTFEKILKDSSSAKNTTLWVRNCQLSFWSLFPSLFMGVLWKDGEIIQKTGFFAGYNWVVWAAILLQAAGGVIVALVINYADNIAKNFATSISILISCIASVYFFDFKVTTSVRKMNALRELNADTAQFTLGTCVVLYATYLYTKPERSPRPPPLGIANFEKTTVDHSPYPYKYDDAQARSPLRSNIASNSTPSTPTTGRFLKRDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN50</accession>
    <name>A0A9W9CN50_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN50"/>
    <dbReference type="OrthoDB" id="5424021at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="179" mass="20142" checksum="DF3178F363DC0D88" modified="2023-11-08" version="1">MANGVSNVWTQMNYVPPRGQCNYKQSVLSPKCPCLRFMLHPLKSSSSYECDGCAHHASFHSMENKDEDALRKRWEQEAREKAEREEEAQSRPKKRVRTIEYNDTVNRLLHESEPDEVATGSLRNTAKTMSPAAKAIAYYKAKEAKAKGPTAKKTGRAAGGRAKNAITEILEEEDIVELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNB9</accession>
    <name>A0A9W9CNB9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRISE-like Rossmann-fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNB9"/>
    <dbReference type="OrthoDB" id="1731983at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08948">
        <property type="entry name" value="5beta-POR_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055222">
        <property type="entry name" value="PRISE-like_Rossmann-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32487">
        <property type="entry name" value="3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32487:SF29">
        <property type="entry name" value="NAD-DEPENDENT EPIMERASE_DEHYDRATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22917">
        <property type="entry name" value="PRISE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRISE-like Rossmann-fold" evidence="1">
        <location>
            <begin position="29"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="429" mass="47981" checksum="0F951F505065DA90" modified="2023-11-08" version="1">MSNQQVVQSNGIFHGLPVYPSSLKGLTAIITGANGISGNYMLRVLAQDPERWSKIYCLSRRPPAIPNGLPQNAEHIALDFLKDSAEIAKVLKEKNVKADYVFFYSYVQAKPKEGGGLWSDAEEMCRVNLALLRNFCEALPLADITPKRIMLQTGAKNYGVHLGPSSIPQEESAPRVTLEPNFYYPQEDFLWSFCQKHNIDWNICMPAAILGSVPDAAMNLVFPLGVYASVQKHLGEELEFPCDLVAWETNRCMSSSKMNGYMEEWAVLNEGAKNQKFNTMDGTTFTWGNFWPKYAKWYGVEYGHPSSDESVYTAIKSKHDPPPRGFGPPATYRVRFTLVSWAKQEKVQKAWEELTKKHGLTGGKLQDMDIDRIFGFTDGGLMGSPLDLTMNKARKMGWHGFVDSNEAIREVLGEFAELKMIPPVGGGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNH1</accession>
    <name>A0A9W9CNH1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_003841</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNH1"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050745">
        <property type="entry name" value="Multifunctional_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24189:SF50">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24189">
        <property type="entry name" value="MYOTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="242"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4372068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="415" mass="46730" checksum="26B6DD561B283CA1" modified="2023-11-08" version="1">MTERALELAEWTALKDFIEECREELAAGAESPACLKAMNVRIPPPPSVKLDVFNAMKRGLPSGHHVSNGTWRALQDETPKPALPFSLRRFILFAIILTAFVSLFLRNKTTWSRPLRRSIADQENGKLEAKLSDPRAVPDPEPAVPITTVRHPPPEIEQTLRRRQVRTHPIYRHGTLDEAIHAADLPEIRTRLKNGEDVNQHWPYLIYRLAISPKADDMAKRIEVARLCLDMGADVNSLKGWNGQSALLIAIHFGNVDVARLLIANGAMVNYSPPDSHLSALHRCVRLAATGSAPDTLAIMEMLFGHGANANQVDRLNETPLHKLLMDSWYARHDEATFEKLFPIALCLVEHGAHIMPESLKAKFITDNPLYEFVQAAIWERSKRLATGREFKRKWQLCTGMDDDGLIAEKVLQLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNL4</accession>
    <name>A0A9W9CNL4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003384</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNL4"/>
    <dbReference type="OrthoDB" id="69177at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="225" mass="25137" checksum="44E8BF0D74871A02" modified="2023-11-08" version="1">MRYYHRSANAASSTPKLCPPVEDCYRQGYDRGGGFNSRKSSYNMAPKKKSKQDSKPIAPSQPAQRSPNWPPLTPLIPESDLAPHEVLPGQIVTISNFWTATLCKNYVAFLSSLPLTTTPGKPKKGDAVRVNDRYQIDDHAFAERLWSETSLKGLVTGATGDDDGLGLSETQRKELWGGEVIGLNPNIRIYRYSKGQFFDQHCTRVVPLERSLGPRMRTMDHADEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP71</accession>
    <name>A0A9W9CP71_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1996 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002856</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP71"/>
    <dbReference type="OrthoDB" id="74764at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018535">
        <property type="entry name" value="DUF1996"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43662">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43662:SF5">
        <property type="entry name" value="DUF1996 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09362">
        <property type="entry name" value="DUF1996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1996" evidence="1">
        <location>
            <begin position="30"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="334" mass="36869" checksum="6F40143E15168B4E" modified="2023-11-08" version="1">MKLYQIAALAAPAQAALRFGCSTLSIQRLDPLVEPGKLPSAHVHQIVGGNAFNATMDKDPSDVASCTTCTFSEDFSNYWTAAMFFRHENGSYKRVQIMENAALPAGINGGMTIYYTQQDFNSNGNQKITAFPKGFRMTVGSPTTNTVEDAKKHPGLRYTCLQDKNTRFPESPDFPPKPCKGGIMTVHHFPSCWDGKNVDSPNHQDHMYNTAKEAFSPAGACPSSHPVRMPQVAYETLWDTTVFANMWPKDGSNPFFLSYNDNKGYGTHADYVFGWKDDALQKAMDSSCMFQACENGKPLKSQNVAAMNKCAVKSSINENIDGWLDHLPGQSMPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPM3</accession>
    <name>A0A9W9CPM3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002571</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPM3"/>
    <dbReference type="OrthoDB" id="5865767at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37283">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN YHR131C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37283:SF1">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN YHR131C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="85"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="422"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="487"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="437"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="487"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="545" mass="59953" checksum="993C4B134FE27FE0" modified="2023-11-08" version="1">MDMAADTTSQSRSRHRSSNPSSSPAAAPTYGYHNTFHTHAQLPIGPPPSFAVAMDSQTLRHLDERTRTEEEVARNTTLPPSYNCTVEIGGIVGIKQELSSPFQVSGNREWYDVYAVLRGTQLSLHRIKNPSILSKNRLPSAGRLIRTYTLQHAEVGVASDFKKTSLIPKSPFAHLVPSAARSKLYETDPHLFEPVREHAIRLRLELEQFLLCPASQEDMLSWIESLCAAIDISSPLEDRSEPRYRSLPRRSRRQRVLDSAQLGDNLENLSSLEAGRRIIAQQEQIIRQLYPHLAGAREPGPIPVASASPAAADLELEEFDPEDVRFPTRPARDSMTRISLSQEYENGEDERPSTGSSSDPKTAPRLRPTPSQILRYRRRCAPVLLASSPRVSDVVYCAGKRVRLNVKEHVLVEYKSHPPRYDAHKFPKTKRPARIATTSPTSTTTPVAGKTTPPVVIGPTRPGSPIRGVSDDSITSISFGEELANARTEQNGGEDTGSSAPPSPTAISHSKADAARQLENMGKRRSEDSRETGLSAVALGVGLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQC9</accession>
    <name>A0A9W9CQC9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transcription factor spt8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SPT8</name>
        <name evidence="9" type="ORF">N0V83_001845</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQC9"/>
    <dbReference type="OrthoDB" id="10260946at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000789">
        <property type="entry name" value="Transcription factor (SPT8), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.120">
        <property type="entry name" value="Large-conductance mechanosensitive channel, MscL, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057544">
        <property type="entry name" value="Beta-prop_SPT8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037673">
        <property type="entry name" value="MSC/AndL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036019">
        <property type="entry name" value="MscL_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30266:SF2">
        <property type="entry name" value="LARGE-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30266">
        <property type="entry name" value="MECHANOSENSITIVE CHANNEL MSCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23798">
        <property type="entry name" value="Beta-prop_SPT8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01741">
        <property type="entry name" value="MscL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81330">
        <property type="entry name" value="Gated mechanosensitive channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="794"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor spt8 beta-propeller" evidence="8">
        <location>
            <begin position="127"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="251"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor spt8 beta-propeller" evidence="8">
        <location>
            <begin position="485"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="306"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="420"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="317"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="445"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="478"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4374769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="854" mass="92082" checksum="FDB7F40141648717" modified="2023-11-08" version="1">MNEEDEFENGASSAEEEDEAEAEAEEADAMDVDEEEEGDDGDDGDNDDDDAEGDNDDEDQDQDQDEQESPSQRPRNSLPNGGTPGPSDANPAVTLTSPSPRAAASGVLGNLPFRPSIRQEALNAPVYDIIPTIAAPHSTSINAITSTPDMRWVFSGGADGYIRKFNWVDTANGKLALTVAQRHPFVDSVTKAGVLLSYWENEDSQNGADTLSPVYSLAVHHQALWLLSGLDSGGINLQSVRHEEGKRIHTLRNHTSAVSVLTLSQDETSVLSGSWDKTIIDWDLNTGQVKRKFETSGGHQISAIEQRPLSTLPIPQDTETLPLSNGTFSSNNAARPRANGSLANGVESSARPAQAKEEGNEDAPGSPEDSLFGDKDSLFGDAQENNPSSALPSFGDDDDEFSRAIANGIQQADEDANADLDMMDLGGPVQAPQEQAGETLKEPSAATQAQSAVNGVTDSQSSGTVNGLPHAEEAGPVANSSAEQTEQPASSETTFVDASINGTLRIWDRRQPNPVARITPPRNTPPWCMNACWSPDGNFIYAGRRNGTVDEYSLHKSLREPRRTFRFPSVSGAVSAVRTMPNGKHLICASYDILRLYDLTQQDPGAKGGAAVPFLIVPGHRTGVISQLYLDPACNFMISTGGNRGWEGASTEVLLGYEIGNDLKSTHLKRTKMPQLNEHTEELLRDAEQGVRHKTTSAWDSFSNFALRDNVLEVAVGLILAAAFTACANSLVSDIILPIISLLPFLARNLDEKFAILKHGPHYNGTISNGYNTIGQALSDGAVVFAYGKFLDKLVRFVAIALALWVVALGYSRGSGDNIVKKQVKCKFCRKYISEKAKRCVNCTSWLDGREERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQL0</accession>
    <name>A0A9W9CQL0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001639</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQL0"/>
    <dbReference type="OrthoDB" id="3935253at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="230"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="379" mass="41740" checksum="7AD2BD238D89D3FC" modified="2023-11-08" version="1">MTTMNTNYTLFASNSKHDTAKGWLKRQLRKASSRQTLKGSDGDRGIHEQVPFKRPQTAPSTESAPQPDTVPTLLPIPNNLERAPTQPSIRPPPRPQRPESGVMRDVSAWLDASMSSPSPPIMAGLSYWRTATVAGVKDSAEIQHAIPIVQEPEADRPSTSHSQQSKLFRRRAKKIQVQMPSLLRTKPQRPAARKQINRRSASMPLLAVLFEEAHIAAPPKLLVRSRSFLRPSNRSSTPNTTTTPLLTQTSTGQQSLERLPYGRRTPASARLDDAETHMEGRINAIFRPTTQGADGTRPSTAAAGLMREDSMGDLSDAPTYFTGPPPPSYRSRTGSILTMSSFGCIDGMTPAQRQLSQQRAAQQRGMKGKMKKFARGFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRC7</accession>
    <name>A0A9W9CRC7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001566</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRC7"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="55" mass="6725" checksum="FC0982C5289BF8AD" modified="2023-11-08" version="1">MSTRKEQEEDEEEDEEEDEEEDEEEDEEEDEEEDEEEEEFCLQDLIKPLSLQALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRE2</accession>
    <name>A0A9W9CRE2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PFA3</name>
        <name evidence="13" type="ORF">N0V83_001476</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRE2"/>
    <dbReference type="OrthoDB" id="302728at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="215"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="12">
        <location>
            <begin position="126"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="294"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="413"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="413"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="462"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="532"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4376195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="606" mass="69060" checksum="5E007664F8A373D1" modified="2023-11-08" version="1">MATLGSPSPPSSPSRTMRRRSWARKVERYCCTAVTYFPLVFVYGLTSWAVWVEAGIGFVPSKNVWTGKFSSALGIFLYLMLNWSYSTAVFTDPGSPLNLNNGYSHLPTTEGGGLQYTSFTVKASTGELRFCNKCQSKKPDRAHHCSTCKRCVLKMDHHCPWLATCVGLRNYKAFLLFLIYLTFFCWTCFAASATWVWSEILSDGQYTESFMPVNYVLLAVLSGIIGIVITGFTAWHLWLTLRGQTTIESLEKTRYLSPLRQAMKQNLNDRNYLDAQANGRLSIGDQLREIHANALPGVTRPEEGEGPSRSTSRSPAPGHAPMSNGYSHPNNQAYEYRERQQNQERYDSYLDERDNEMLPNAFDLGWKRNIGHVFGPSPLRWFVPIANTTGDGWSWEASPKWLAARERIKRERAREEQIQKQRERAAGWGVDSPTEAEFRRPTRTPEGWQPTQYSQPVPRQPRRPEWQSPLRHDEQAQYLTTSNGILRSPREGRRSPSKADQILGRENGMYSDGDVQLQNIDRRKFDQYNYISEDEDDDYEVSSDEQAADRKRSEQVKTVSAPPKGTMNWNDIPEGFLDPAPKGKKSTSRERKAGPQKPAGWDDWGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRL5</accession>
    <name>A0A9W9CRL5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Trimethyllysine dioxygenase</fullName>
            <ecNumber evidence="5">1.14.11.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Epsilon-trimethyllysine 2-oxoglutarate dioxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">TML hydroxylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">TML-alpha-ketoglutarate dioxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">N0V83_000896</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Converts trimethyllysine (TML) into hydroxytrimethyllysine (HTML).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(6),N(6),N(6)-trimethyl-L-lysine + 2-oxoglutarate + O2 = (3S)-3-hydroxy-N(6),N(6),N(6)-trimethyl-L-lysine + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:14181"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:58100"/>
            <dbReference type="ChEBI" id="CHEBI:141499"/>
            <dbReference type="EC" id="1.14.11.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amine and polyamine biosynthesis; carnitine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the gamma-BBH/TMLD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.8" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRL5"/>
    <dbReference type="OrthoDB" id="408743at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050353">
        <property type="term" value="F:trimethyllysine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045329">
        <property type="term" value="P:carnitine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00250">
        <property type="entry name" value="CAS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2020.30:FF:000002">
        <property type="entry name" value="Putative gamma-butyrobetaine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000001">
        <property type="entry name" value="Trimethyllysine dioxygenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2020.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050411">
        <property type="entry name" value="AlphaKG_dependent_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010376">
        <property type="entry name" value="GBBH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038492">
        <property type="entry name" value="GBBH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012776">
        <property type="entry name" value="Trimethyllysine_dOase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02410">
        <property type="entry name" value="carnitine_TMLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696">
        <property type="entry name" value="GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696:SF51">
        <property type="entry name" value="TRIMETHYLLYSINE DIOXYGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06155">
        <property type="entry name" value="GBBH-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0124">Carnitine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma-butyrobetaine hydroxylase-like N-terminal" evidence="18">
        <location>
            <begin position="55"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="TauD/TfdA-like" evidence="17">
        <location>
            <begin position="153"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00071191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF06155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ4378066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="417" mass="47219" checksum="5D73BEDB22064440" modified="2023-11-08" version="1">MDMRALSKELKGIQRSQRGYAKIFRGPYGVCNGTIYKDRVRNFEVQDGKVVVGERTIPNIWLRDNCQCSSCMHDATKQRLLDTFSIPKDIAIKSHTIGQNVLKVEWSDGHSSEYTRPFLSHATRSAEFRAILRQGLTPIKLWDSSIASEAPTENYAQTSEGDVAQILKNIRKYGFCYVDGTPFDDPAATEFLLKRIAFIRETHYGGFYDFTADLASKDTAYTNIALEAHTDNTYFSDPAGLQAFHLLSHTDGEGGASLLVDGFKVAEEMSRTDRKAYEILSTVNVHAHASGNEGISIQPYRSFPVFEHDTATGDLLRVRWNSSDRASIELPIDEVEIWYDAARKFDALLKKKENEYWEQLTPGRVLIFDNWRVLHGRSKFTGKRRICGGYINRDDWMSKYKMAEFGPKTVLGTLASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRW1</accession>
    <name>A0A9W9CRW1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRW1"/>
    <dbReference type="OrthoDB" id="3684856at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="141" mass="16178" checksum="9FC46C8547AE45AB" modified="2023-11-08" version="1">MPSENTHNTTANLKQESLEDPTALQEPQTTQESQAAKESYADYIKNLPPPFWETKTPQTKVANDEAIAARVNAVCAPVTAEQREEDERLKAMSWRERWRDRKARREAKEKNKDTDLRPVERGSRAQLNVFSVNIKGKQGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CS44</accession>
    <name>A0A9W9CS44_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000551</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CS44"/>
    <dbReference type="OrthoDB" id="5357513at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19071">
        <property type="entry name" value="AKR_AKR1-5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000045">
        <property type="entry name" value="Aldo-keto reductase (AKR), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="25"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="302" mass="33818" checksum="12FD8FD4B3D0DBF4" modified="2023-11-08" version="1">MMASASHGTASRLHDIEPAFIYGTAWKKDQTNRLVEEALVAGFRRVDTAAQPRHYQEAQVGEALRQAYRDGVVKREDVYVQTKYTTPAGQDVNNMPYDPSAPLETQIHTSIASSLRNLRSEEESEEDAYLDCLLLHSPLPTVEQTLQAWTLLESYVPNRIRSLGISNVTLPILQAIYQNSTVKPSVVQNRFYPQTRYDVPLRRFCGEHGITYQSFWTLTGNPALLKSKPVGTLAQATGVDPSLALYALVMELGIVVLNGTTSSAHMQEDLEGIRKVRQWAQSYPQEWTGISHAFRSLVEPVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CS84</accession>
    <name>A0A9W9CS84_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000703</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CS84"/>
    <dbReference type="OrthoDB" id="5424391at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="429" mass="47637" checksum="AEA8C7438E804785" modified="2023-11-08" version="1">MQSSAASKRLLSTLASKIHPQLPLSPRESQQLLNLLTTSFRAHLDREHPLNLPESSRPRAQQRPAGSNGQRSPSPTRATSSYASATRHMDSILSNPLFAIRPHRSTSEPAAVDVLRDPMTWFLHEIAIGAADLPKAAMCLEVLANTATEASPGLRDGRTPASILAEWLKTSGLDCSKQFLEFCVTKDGKSSVFLQRLVKLLLADGQAEAPWRWFIRSNEKRINETGIDHGKAMTFKKQLLARMVSDEATTSLDKGMATFLQAFRIAQVSGHGSAYTILKPAGAQLVNRIISNPDQSRDLGLYQSFLQSCQLWLGSWSSAVEPMLWLHHPDQSSAQPGLRFIQAPTGAITFAKTTRQSQRRFLVQLCLGVARQLMEQERYAEAQIAMEFTKEHFADIVLSKVPVVQQTTSRSKERMERKNLELLEGLALT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSD0</accession>
    <name>A0A9W9CSD0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VHS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_000838</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Component of the ESCRT-0 complex composed of HSE1 and VPS27.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSD0"/>
    <dbReference type="OrthoDB" id="10068368at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14232">
        <property type="entry name" value="GAT_LSB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16980">
        <property type="entry name" value="VHS_Lsb5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044103">
        <property type="entry name" value="GAT_LSB5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045007">
        <property type="entry name" value="LSB5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47789">
        <property type="entry name" value="LAS SEVENTEEN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47789:SF1">
        <property type="entry name" value="LAS SEVENTEEN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VHS" evidence="3">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="118"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="293"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="323"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="347"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4378008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="379" mass="42519" checksum="191A60D4A809856C" modified="2023-11-08" version="1">MPYEENDIGGVVDLIEVIRIQSSGPTEAARALRKKLKYGNVHRQIRALVILDALIQNAGTRFQRAFADEPLLERLRILARDDMVDAEVRQKVNILFRQWAVAYKGTPGLERIAALHKELPRTKRPQPQQSRIVRQQDAEAAREHEEHSPPPSPPKSRRPEPHHASSSNRPVALGTASFPSSSIFKKDKKNKKTRGQPFNLEKEKGQLLETIASANVASTNLLNGLQLINREQQRVSDNSEVMRRFETCKQLRRQILRYIHLVESEQYIGGLLNANDELVKGLMAFEIMDKSIDDDSDSDNELSTSPSAESAMAGLSVNESAPVKPPRPMSIPMPPAQMPGKQRADPDSEPDVDEDDPFGDQNAVKTPWAEKGQPTWRDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSE3</accession>
    <name>A0A9W9CSE3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">T6SS Phospholipase effector Tle1-like catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000868</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSE3"/>
    <dbReference type="OrthoDB" id="59699at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018712">
        <property type="entry name" value="Tle1-like_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33840:SF16">
        <property type="entry name" value="DUF2235 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09994">
        <property type="entry name" value="T6SS_Tle1-like_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T6SS Phospholipase effector Tle1-like catalytic" evidence="1">
        <location>
            <begin position="11"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4378038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="512" mass="57814" checksum="3D211D9D54829AF1" modified="2023-11-08" version="1">MPTADSTRSSKQLLIFCDGTWVGRETNVANAPASNIRQLANMVGDVQYGSGPAQDPSKVHIIKPHATSAMHFNPYARGVNGSAKSHIIAGYQEGVGVDGTFLEYIWDGATASTIGDECISVYKFIVEHYTSDNDEIWLFGFSRGAFTVRCVAGMINNCGVVKRLPEYTSEEVDRLCYEVFRTYRSNLPNDAPKSEACQRLRGNAQRVWQVQRPIRFMGCIDTVGALGIPRLNAGIGFDWSPFEFFDQHASSVIQYVYHAPSLHDRLWAFQPCLIYPGYEDGKVRPVVKQRWFPGTHYDLGRMTFRFVRQHPSNWIEGLLGWLPDLLSKTIYPNEILSDCVLRWLLEGIQEVDSNSHDPIIPDVKGQIQRISERMANADGKSTGSGDIYGDVLNYAPAGIIWGSIQRASQFFFSLLDRILPHLGSNIQDLLGIKTILGILTATADRRIPGFTADVYPYTEEERVRVKGQERVFSVREQARMRGQSEWGKERYPSRTFETFLLWRRVFGPGRTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSE4</accession>
    <name>A0A9W9CSE4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000719</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSE4"/>
    <dbReference type="OrthoDB" id="3940486at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="79" mass="8734" checksum="F8504417279394F6" modified="2023-11-08" version="1">MAPTTTKTATPKATGGAGVKKAGRPKAKAKNSKARTAMIKMQAYFKDNRSKYKDLDFKEQQKELGKDWKTSSENPKNQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSI2</accession>
    <name>A0A9W9CSI2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000839</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSI2"/>
    <dbReference type="OrthoDB" id="5125733at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112:SF10">
        <property type="entry name" value="TOL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4378009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="42573" checksum="AC3CE32CCC4E8857" modified="2023-11-08" version="1">MADVYSNAYLTIAATRASHSRFSDEEGSFELIFNYDDRTSFPGSMETVTHQPLRVHKGEPLLERVWCFQERVLATRTLHFASDQMYWECAHCFESEEGMVNVREEDFYREYSMEKIANGLRNVAGIEDPTRRRDAWFLLIEEYTSRDMTYQSDKLPALSGIISALQNLTGDTCYAGIWKSWFMKGLLWRLQSPEWDLYVFTPKQPFKLDFYRAPSWSFASIEGVALYNEVDYVDAGNFCAQLEDCSVAPKGNNPLGELKSGFARIIGPVTSIVSVDHKVSRSGRACKVLLRDQRPVAASVYFDFDPYDSCQILMITPHTGIAITPVGTEKDTFVRVGAVSVGRVWDDVELSRASERRGRYLTALAFPPPTSITLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XX52</accession>
    <name>A0A9W8XX52_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Dynactin subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">N0V83_010749</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="13">Subunit of dynactin, a multiprotein complex part of a tripartite complex with dynein and a adapter, such as BICDL1, BICD2 or HOOK3. The dynactin complex is built around ACTR1A/ACTB filament and consists of an actin-related filament composed of a shoulder domain, a pointed end and a barbed end. Its length is defined by its flexible shoulder domain. The soulder is composed of 2 DCTN1 subunits, 4 DCTN2 and 2 DCTN3. The 4 DCNT2 (via N-terminus) bind the ACTR1A filament and act as molecular rulers to determine the length. The pointed end is important for binding dynein-dynactin cargo adapters. Consists of 4 subunits: ACTR10, DCNT4, DCTN5 and DCTN6. The barbed end is composed of a CAPZA1:CAPZB heterodimers, which binds ACTR1A/ACTB filament and dynactin and stabilizes dynactin. Interacts with ATP7B, but not ATP7A, in a copper-dependent manner. Interacts with ANK2; this interaction is required for localization at costameres. Interacts with N4BP2L1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Stress fiber</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Myofibril</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the dynactin subunit 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XX52"/>
    <dbReference type="OrthoDB" id="283815at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008603">
        <property type="entry name" value="DCTN4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13034">
        <property type="entry name" value="DYNACTIN P62 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13034:SF2">
        <property type="entry name" value="DYNACTIN SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05502">
        <property type="entry name" value="Dynactin_p62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="161"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4361808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="578" mass="63919" checksum="3328B099BEA9570C" modified="2023-11-08" version="1">MAQTFPYTYYACDCYDSNTTTSSKRASQLLADAEAENDTFNPRNPRSNYSLYPLEHLLYCEDCQQIRCSRCVIEETLNWYCPSCLFEVPSSVVKSDGNRCTRNCFNCPVCISPLTVNVLESPDASSGTADRHILACPYCHWSTVETGIEFEKPTGVYGQLARKGNHGKTISTHKERDKDREWQKELTARKRGEGVTPSAPDEAAAQKEHEPPTRDELFSHLGAFYKSQLEAHTPSNPFSPHELNFSSPSAYSRIMNLYSTAGSKKQKRGKPTPMREAAFEFEGLAVHDPATDTAAIERIKRDGWNSTLSVAQKLAQVNPFAQFDDELRPIPTLLCTKRSKRCRSCRHILSKPESKITSTRYKIKLLALNHIPRISIRALPATNPGSAVPPASGSSSSQFSYSSLRSGIPTHFLLHLSNPLFDPIKVTLATPSTTQGKVQSRVTILCPQFDVGANTDVWDDALSSSVPPVNRSSVLNADGQVEAGKVWGKGRNWTSVAIEVVPGFLKPFTEFGGSSDGGDELNEDDDLLEIPIFVRLEFEADVNAEERGLGDSRGSKGEKERREEAFWAVLGVGRIAPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXH6</accession>
    <name>A0A9W8XXH6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXH6"/>
    <dbReference type="OrthoDB" id="3801562at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="613"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="622"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="693" mass="77030" checksum="AF99A1CC0E64BA09" modified="2023-11-08" version="1">MGNAESSPNTNPAAFDDKRRELSNPELSSVSSPTAHPGDTSALGDQAIQKSTPQAELPTTFRIASLDNRNQPPFLATTEATALDVSKKDIGVDNDGNKLGTLKGFYNARDRAKGVEATGRSNNPFEAARAKDPGGFLFRYPAPPPATSSLSPQPQQGTVFSSSANQTGNPTTLNLAPNIFAEARLKLAAEKTTPLASIVSKEEQSAPTPSTPFTFPIAQSFTAKPFQFSSQPATISPSNQKHGRSQSDGVAFAVESSDIDKFFSLMMQGSEPLDATSIVELGHTALPLDDFFKWVGEAIDLEAPEMVPLKENLKELHALTLAFIVDISKLAVVIYGKKPILMQWRKMISEYERELEEEPLTREKTMCRIKLWQGAIVRLEGAITEAKSTVDKAGRDFARVVVKPLLSYSSLTKQFWEAQLDEKNNHIGALRTKLLKLEQDLTNETGRLNTKMLKLEQDLRNEMGRLSTELVKKEAILSVLHNEYRSKMDKKIAEKNQEKADALRDQMLKVKIQLTEAEETKVQHKKAMTTTRDLEEKVKRVEKESSNLKTVKAAREKELLESEAQVKQLKCYKEAHDQIKTEFEELQISFNDVATERDELLKRVQELKAELAQKAGSVGSLRPSSETAASATEKEEEEPPARAGTSKEEHLAGLKTYWEHRLKTALQAIEERKASIAWHDAEIARHRGDKRQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXV9</accession>
    <name>A0A9W8XXV9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010616</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXV9"/>
    <dbReference type="OrthoDB" id="3880384at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="307" mass="34669" checksum="24BD594AFE57DF1D" modified="2023-11-08" version="1">MTPNLFLATQRVFSPHKWFKTSKNERAVRQTEFWEDGPVPGLYEYIPGRGRYLVATVKEASTNVTDSKTSEGGPVLPPSSPQPREYVHLEHPIPITYSRAIKRWFLDPDYKLRKKHGVITNNRGKRVTVGFFRTDDHGGVAWVQCWDEEGTFVPGPYQKWCIDAETKQFRHMRKRDDPNYVQSRSNSSSRDADTHSQDSMSTQIRDSLSGSTKNGPSPSLPSTRANSIRYMPSSNSASRPTSQRPSRQTSPRRNNSIPLEEAKVSLRRMAKEHQEAVAAAAAKGLRADSQDRVERIDRGRPAACVGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY47</accession>
    <name>A0A9W8XY47_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxymuconolactone decarboxylase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_010897</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY47"/>
    <dbReference type="OrthoDB" id="5537330at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052999">
        <property type="entry name" value="PTS1_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28180">
        <property type="entry name" value="CONSERVED MITOCHONDRIAL PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28180:SF5">
        <property type="entry name" value="DNA POLYMERASE ALPHA SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4361956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="28449" checksum="9BF2D7ADDB209061" modified="2023-11-08" version="1">MATNELPIPRDPTEDEALALFKALEEKFPSKTLGDDKWYILAFAAIVGGGQPSFAPLLYRELIKRPEFQTPEQRQALMRRIRETLFKLIVIVGVCKPLEAIFDIDAITRPEDKDYTFSREGWQCDEANAKRGFEWQNRIYQHNQANIDNVLASQRDFDWTSKQITYGLYLSDQSILNDVETELTVLSGIMIQNLPRETAWHLRGTRRIGVSKEDVETIQQCIELVARFCNLRLHKVPRVADIEHEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYL2</accession>
    <name>A0A9W8XYL2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_010580</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYL2"/>
    <dbReference type="OrthoDB" id="4161376at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06179">
        <property type="entry name" value="MFS_TRI12_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010573">
        <property type="entry name" value="MFS_Str1/Tri12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053791">
        <property type="entry name" value="MFS_Tri12-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF195">
        <property type="entry name" value="PEP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06609">
        <property type="entry name" value="TRI12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="411"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="53"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4362486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="584" mass="62492" checksum="7759CE6E63FEF305" modified="2023-11-08" version="1">MAVPNEKDEVRRTSNEVSKTSNSLKEKDAENPMVGYLPQSDEEYKVTLKTWCVVLILALSYGISFWIVPSLSACAAVVAAQLGDATKSAWYISLYTMTVTIAFMVCGANSDLFGRRWFIVGGNAILFIGFIVGGTAKNNTTMLAGMSLIGFGAGNAQLAAFALPELLPNKWRAAAIVLADLGVYVAVLVGPVAGGFAAQNGDSWRWLFYSPAIAVFFSFLGLFFFYYPPKHPRGLPTKQALKELDYVGATLFIASATLILVGIVYTTTLPSKDPKVIGTLVSGFVTLVAFAIWETFAPLKQPLTPTHVFTRDKGRELTAPFIVGFVVTMFYYAVNIIYPTQIATLFTDATTDYKYRIVLGLPGNLGLCFGAFLLTVLGHRIGHWKWTLTGSVTMMVVFGALLALGNPQRKGMMIAFVFLCQVGFGWAQYLSIAFIQFGVPQVELGISGGLAGVSRFAGGAVAISVYTTILTNVQSSHALKLVPPAATTAGLSPSSVPALLAALPLGATALEKVPGITNEIIAAAGAAFQEAYVIGLRTTALSSLSFGILAIIACCFTNDIGPKMDNKIEVFLENDENAGKNKYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ45</accession>
    <name>A0A9W8XZ45_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">54S ribosomal protein L12, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MNP1</name>
        <name evidence="7" type="ORF">N0V83_009766</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ45"/>
    <dbReference type="OrthoDB" id="250175at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00387">
        <property type="entry name" value="Ribosomal_L7_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.10:FF:000001">
        <property type="entry name" value="50S ribosomal protein L7/L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.710">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00368">
        <property type="entry name" value="Ribosomal_bL12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000206">
        <property type="entry name" value="Ribosomal_bL12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013823">
        <property type="entry name" value="Ribosomal_bL12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_bL12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008932">
        <property type="entry name" value="Ribosomal_bL12_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036235">
        <property type="entry name" value="Ribosomal_bL12_oligo_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL12M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00542">
        <property type="entry name" value="Ribosomal_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16320">
        <property type="entry name" value="Ribosomal_L12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="ClpS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48300">
        <property type="entry name" value="Ribosomal protein L7/12, oligomerisation (N-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein bL12 oligomerization" evidence="6">
        <location>
            <begin position="50"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein bL12 C-terminal" evidence="5">
        <location>
            <begin position="118"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4363472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="185" mass="19608" checksum="F1B24E3224CFF937" modified="2023-11-08" version="1">MSAPVSRALRRCICNAKSTTYVRPTMGATQQRIQRRWASGDAAAAPSNPKIATIVDQISQLTLLETADLVSTLKTRLNIPDMPMGGFVAGPGGSGPAAAAAPAEEEEAAPAAQEKTLFNLKLEKFDAGAKPKVIKEIKAMLGLSLVDSKKFVESAPKMMKEGVPKEEAEKIVETLKTLGATVVME</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZA9</accession>
    <name>A0A9W8XZA9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_010931</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZA9"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4361990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="135" mass="15208" checksum="11497B3787CF297F" modified="2023-11-08" version="1">MWASYIPVALNSSKTNGTKEFAYQLEYATRAQYLLPDVLGKNSIYECQNETHIGLEGFSRKMNNVASSLSNSNAPPWKSSILALLRCMDLENKLGTPQQVSQYGRETLIRLQVVNDGETWHMLDTTPSREAHAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZG7</accession>
    <name>A0A9W8XZG7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZG7"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040942859" evidence="1">
        <location>
            <begin position="19"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="90" mass="10066" checksum="D0AAD97241A4F47F" modified="2023-11-08" version="1">MKLFMFFLFVMLLVLTVAVPPNGQLLKSLDLRDKPKCSDGFYRCYLYCVFGHSSDVCFSTCQKMWCSAGAEDNAGADTIEIQPEDPATPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZP4</accession>
    <name>A0A9W8XZP4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_009526</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZP4"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018455">
        <property type="term" value="F:alcohol dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="8">
        <location>
            <begin position="9"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4364071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="317" mass="34482" checksum="95F51443569D2C11" modified="2023-11-08" version="1">MKAGQWDPKAQKVVINDIAKPTAGPNQFLVKIQSASLCHSDLILDLRPDYAVTLGHEGVGHIESIGDAVPTKDFKVGDSIGFNYFIGCCFECDGCMIHNLRCETGNQKLQGFYVDGFFAEYAVVDWQNAVKLPETLDMSKSAPLFCAGITAFHSVDSCELKAGDWLAVIGCGGLGQYAIQYAKAMGIKAIGLDINDSQLEMAKKVGADAVFNSMTNKNYLEEIKKLTGKGCHAAAVYSASNAAYAGFDFVNTFDLTTGKYRIKADSTGIPQRMKKAVDFTGKHSIQPEVDFRKIEDLPQMVADMQAGKADKRQVVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZY2</accession>
    <name>A0A9W8XZY2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009764</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZY2"/>
    <dbReference type="OrthoDB" id="3830579at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4363470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="55" mass="6240" checksum="D9268974B7FE0054" modified="2023-11-08" version="1">MAVTEIACMGIKPGLNVMDDTTKEGQILHKVEDQSKVWAFFDWESVEEHEKFAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y094</accession>
    <name>A0A9W8Y094_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">BAR domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">GVP36</name>
        <name evidence="1" type="ORF">N0V83_010109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y094"/>
    <dbReference type="OrthoDB" id="5549748at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07600">
        <property type="entry name" value="BAR_Gvp36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018859">
        <property type="entry name" value="BAR_dom-cont"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10455">
        <property type="entry name" value="BAR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4362992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="319" mass="34833" checksum="356C9D47E068BBEA" modified="2023-11-08" version="1">MDNLGKISAFGKNISASFTPWAARTQQYVKEQVGQVDDKTQLPPDYIELEKRVDALKKVHTTMLQVTSQYSNEAYDYPANIRESFNDLGRTVSEKVNLLSSATSPAEAQAALTAPPSAKPQPKTFSHAVARAALNASHMLTSEQTGSSEDPLATGLEKYAIASEKVGEARLAQDAQIQSRFLAGWSTTLNTNIQFATRARKAVENARLGLDATKASAKGPAFALPGQTGRKDGVEEDLSEEARAKIEQAEDEFVGQTEEAVGVMKNVLDTPEPLRNLADLIAAQLEYHKKAYEILSELAPVVDQLQVEQEVRRHQSSDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0J2</accession>
    <name>A0A9W8Y0J2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009236</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0J2"/>
    <dbReference type="OrthoDB" id="566238at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01519">
        <property type="entry name" value="RHOD_HSP67B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE RDL2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086:SF10">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE_RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="99"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="250" mass="27183" checksum="5A62A4D2C6CD3AE2" modified="2023-11-08" version="1">MASKRTVQLLSLRCSNASIVNRSRQTPRLQLQAAARALSVASRPHCQYALKFAFERAPVGGVSKSQVGFVQQRWHSRAPADEKSKVYEFEDVLAILEQPSNSRLLIDVREPHEYEANTIPTAINIPVTSQPDALLLSEEEFMDRFGFAKPPTNKEVVFFCKAGVRSSASAGIAKQAGYTNVGEYRGSWLDWERKGGPGTKTPPPPGGVGEPKAPVSEMKPTERTGSSGEEDLGPAGQPKYPDGNLMGGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0N9</accession>
    <name>A0A9W8Y0N9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009470</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0N9"/>
    <dbReference type="OrthoDB" id="3928002at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38049:SF1">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38049">
        <property type="entry name" value="RICIN B LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040875645" evidence="2">
        <location>
            <begin position="16"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="305" mass="34555" checksum="99E11152938CE474" modified="2023-11-08" version="1">MALPLGIFLGGCVVAIPVVTGIAQGVEHQKKQNEEAANESRMWKFNCLVSCDDSDELAQDELNNGILVVRHEKVWVVPRDGDNKPIPPGEALELKGVAIDPALGPLHAFAGFYIQYPDEDRCPPERGLVSTISDDPPMLNWIYCDRTTYELRHSNRTGSITHHVGDWDWTEDETHLMFDGWEGFVAIDEWDGADSDPSTPWGREGLRWAVYFDRDDNGLKGRRKGRDMFEISLKRRIQSAEEQLKQNEAAEKKMQVKSRGGLSTQFSAPTKERDKDWEKKYGEQARLELEKKLAKDKAEGQGPSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y145</accession>
    <name>A0A9W8Y145_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cytochrome oxidase assembly protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">COA1</name>
        <name evidence="1" type="ORF">N0V83_008667</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y145"/>
    <dbReference type="OrthoDB" id="2100652at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014807">
        <property type="entry name" value="Coa1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042432">
        <property type="entry name" value="Coa1_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28523">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28523:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08695">
        <property type="entry name" value="Coa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4365051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="226" mass="25741" checksum="7FB958A03A201521" modified="2023-11-08" version="1">MPPPRLTSALFRAPAQIRLQCLRRLAPLRYASTAPTNGDASTPVVPWKTPTSVWSPENLIPPPKDGEILMERKPNRELPPVPPLIPTQVRNTLPIFIIAMTISLFAFFNYQKTESSVVTSTLYALRTNPLVRSELGDEIYFASKNPWIKGEINQVHGRIDIRFWVKGTKSMGEVRLRCRRRGRGGLYYTQEYSLTMRDEDGSDRRLELYDPKGNAIDPFQDPIGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y188</accession>
    <name>A0A9W8Y188_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_009289</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y188"/>
    <dbReference type="OrthoDB" id="2962993at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000034">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000068">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF57">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="415"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="44"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4364692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="476" mass="53367" checksum="196FD456FC531275" modified="2023-11-08" version="1">MAEKKLSTDVAIAASPDIDAGDIEPQGFDHAATKKLLRKLDWHIIPFMSLIYLLCFLDRTNIGNARLDHLEQDLKLHGLQYNDCLAILFPFYIAIEIPSNIMMKRTRPSIWLTFIMFFWSLAMIFQGFVHDYSGLMATRAFLGVFEGGLFPGVNYYISQWYRKSECGFRMALFFSAATLAGAFGGILARGIAEMNGVGGKAAWSWIFILEGLLSILVSIFLTSKEKTEVERRLAEDHGPLSNEFDMKYVWQAVTDWKIYIFMLILMAGFCPIYSFALFLPTIIKNMGYTANDAQLMSVPPYVCACFFTIAASWYADKVRKRGIFLMGFQLVGIAGFSMLVATSKPSVQYAGTVLAAIGIYPQIPLGMAWNSANIGGSLKRGTGIAMQVMGGNCGGIIASYVYLTRDGPRFITGHSILIGFVSMAFFLTFFMSTWCRMENARRDKVALEVGTQELTEEQRLLERELADNAPWFRYTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1P8</accession>
    <name>A0A9W8Y1P8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Six-hairpin glycosidase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1P8"/>
    <dbReference type="OrthoDB" id="3534988at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040765645" description="Six-hairpin glycosidase-like protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="704" mass="79641" checksum="82ED51644175D1FF" modified="2023-11-08" version="1">MPSIQLWGKALLLLFVASPFVESKIDRKSLVQQYNLKLNHSHPYSPVQVGNGNFAFGVDITGLQTFLPHNTLSSWGWHNSSLPSTPNQTSPTDYTGVDWWTHGRLVNYEQPDPAKKDISQWMIANPHRINLGRIGLWWGNVGRGNVSEEQLSDKYQVLDLWEGVVASSFSIDGEEVLITTVASPETDTVAVNISSTLVRTEGLGIYLDFPYASGKNKFDAPFVGLYNATSNHTTSTQYRNRGASITHTLDATTYVADIAWEGYARLARLHEDEHKYILAASQNFETLTFRVTYSPQVVNISSTFATVKDEAATWWPEYWSRGAFVSLPTATNSSANELQRRIILSQYLLAVNEAGKDPPQESGLVNNGWYGKFHLEMVFWHLMHWMFWGKWDLYDRSIGVYERFLPTSFGRAKNQGYEGARLGKMSDPSGRSAPGEINSLLIWQQPHPMYFAEMEYRNFPTTQTLQKWDHILTGVADFMATYAWWNSTTGRYDLGPPMYPVSENTNPNQTVNPTFELAYWRFGLDIAAKWQVRQNKSAPEQWTHVRDNLAPFPTEDDTYVTYEGIPNMWSTPEYTEDHPGLVGIYGWLPPDSRLNMTIFNNTIAKVHETWNFPFSYGWDFPLLAITEARRGDAERAVQQLLDVNNAFDEVGMPVGGTRVPTPYFPSAAGLLLAVAMMAGGWDGFEGPVWPEGWEVESEGFIRGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1W3</accession>
    <name>A0A9W8Y1W3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_009706</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1W3"/>
    <dbReference type="OrthoDB" id="2151789at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF22">
        <property type="entry name" value="FAD-BINDING PCMH-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="5">
        <location>
            <begin position="36"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4363413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="475" mass="51566" checksum="F1C8993F0EF85191" modified="2023-11-08" version="1">MDIDTTLSPVLPGKILFPNSEHYDTSNNSYFTLFENDLRPAAIAQPTKNAEVHTLLKILKPLAEKHQIQVAVRGTGHTPIAGSANTADGVTIDLRGLKGIRLSEDKSLVEIGVGETWGSVYEELEKHGLTTAGGRVGRVGVGGLVLGGGLSLYSTRRGFACDSVTEFEVVLASGEVVRASQDENAGLWRALKGGLNNFGIVTSFKMRTFASGDIWGGIAYYMPDTFGDLVKATVDFVDNESDEDTHIMSSAGYGFGHQVVTCCMYQTKGVENAASLQRFTSLPSQIKEHGSLRSSTHIDFCNELSKFTKDGVRSFYATLTIRPDVSLMLAVHQAWQGTLDSLKDAEGFIFSLGFFPLTKALLQNSQDAGGNAIDIDPNDGPLLIVLINPTWDSPSDDERIYAGVESLLGKCRAIASAKELLHRYIFTNYAYFKDDVFRGYSDSSLAVLRKTSKEFDSHGFFQNVVSGGFKLCTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y219</accession>
    <name>A0A9W8Y219_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_008407</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y219"/>
    <dbReference type="OrthoDB" id="21204at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46077">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TOPORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46077:SF1">
        <property type="entry name" value="TOP1 BINDING ARGININE_SERINE RICH PROTEIN, E3 UBIQUITIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="16"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="75"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4365786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="264" mass="29948" checksum="675C72EDCA5967CB" modified="2023-11-08" version="1">MGSTAGSTKDESEDACVICLSTITERALTVPCNHGTFDFVCLISWLQGRSACPLCKTEVTAVQYDWQSPTGFKSFPIQRSHPPKHTSSSVPTRPSLFASYGLPRRPRGARRPYSPPVEDLALRRRREVYRRNLYSFHVGSNRFSGYRDITPQMVASSSDLQSKARTWIRRELRVFSFLNTGSTGSTATDLTTTSNAEFLLSYIVSILKMVDVKASNGHAENLLTEFLGREYSRLFLHELSAWLRSPFTKVDEWDRNVQYEQDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y269</accession>
    <name>A0A9W8Y269_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009874</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y269"/>
    <dbReference type="OrthoDB" id="3796126at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="574"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="788"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="428"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="806"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="875"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="893"/>
            <end position="902"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="938" mass="103597" checksum="4E26A56125EE052F" modified="2023-11-08" version="1">MSSHHDDQKKKRPPPIKMPPDPLPDSPQARRGGKQQVDARKASGSTQPEPRSASSTHISATSSREQSGSYLPRSQSQVSKSNNSAKTTFSNLMVQARGSPRKSELGSTTNSRHGSTTRSRHSERSHQSAASAQAQLEALDEETSRGKIESQHEQRLFKMTGQLPPTPTIGTTNEDDVYIRTEDLRAQCRAASGEKQAPSDEPLKSPKKKIFGNMQMFNPFNRTANNTPAPTMPSKAAQVLGTSERKPHVIKVRPIKPSQPYRTPTRVPRSDTSKSLPGKAVDPHGYARRHYSGSSRRNRTTGERSGRDSKQSLNAENTPPMPENPSFESVPPPTPPAKDTPPEFKAPPSPLRRALPSEDLRESYEAHVDRNMKLHFPEFALSPSPSKTARPGSARSSPSKFRSYTAEDYTKLIEGEPLQWHYPEEDGSSGQQGSKRNASLANETLEALHLRRSDRTSDGQQYDDPDSRRWSVLPPRFYSPSNRSVRLFTEGETPSKNTDADHLLFSPPSKAKLLHLREDSNNGSIEMIFQGNPDDIDPRSSTGQLLNDRVQNMTASQARDNTRLTTRVMQELQINEPNDGDPQKGSASALGQPEPSSSRLTDMLEGLTPRRAEFSGNFQPNCPSAVPSPLNPMSDPLVHSQAHLIPVGVSTFTPGAPPRTPKTVEDHFFMTNEHLDVVAKTTWDLLEISKAQQSTTIDDKQDQLMGSIGKRIEEVRVQIETANSKTDQASVQTASIHTNLEQLTDFIKQDVMNALVDQNKRTASMETDIKELQKTMQALHKLLEQKFPEPKSTGQQHSATGALPASVSTQSPYHLPTHRSQPSLAGYYGNTPDLSREGQPPMPQMPQMHDNRSAVPPQESHNDLRAAYTNNYGQQWGGRTGYSGRGSKEERPPYSGGNPYNYGNGGQFSNAYAGNYSPYNFSPSPPDQHYAFNQGPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2T4</accession>
    <name>A0A9W8Y2T4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">mitochondrial processing peptidase</fullName>
            <ecNumber evidence="5">3.4.24.64</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Beta-MPP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">MAS1</name>
        <name evidence="18" type="ORF">N0V83_007855</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalytic subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins. Preferentially, cleaves after an arginine at position P2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.</text>
            <dbReference type="EC" id="3.4.24.64"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.64" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2T4"/>
    <dbReference type="OrthoDB" id="10251424at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000002">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000001">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF149">
        <property type="entry name" value="GH01077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="16">
        <location>
            <begin position="56"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="17">
        <location>
            <begin position="208"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4366219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="484" mass="53670" checksum="1C6526ADEF4E5F45" modified="2023-11-08" version="1">MASRRLALNLQQSMRSRAAINAVKSSRQSPLTRGLATPVTYGSKTESTTLKNGFTIATEHSPWAQTSTVGVWIDAGSRAETDQTNGTAHFLEHLAFKGTQKRTQQQLELEIENMGGHLNAYTSRENTVYYAKAFNNDVPAAVDILSDILQNSKLEPQAIERERDVILREQEEVDKQLEEVVFDHLHATAFQGQPLGRTILGPKENIQSIQRADLENYIKTNYTADRMVLVGAGGIPHEQLVDLAENYFGSVPSEPQDYTSKSVAAEQKAKPDFIGSEVRLRDDTMGTANIAIAVEGVSWSDPDYFTALVTQAIVGNWDRAMGNSAYLGSKLSNFISQNNLANSFMSFSTSYSDTGLWGIYLTSSNLTQLDDLVHFTLREWSRLSMNVTSAEVERAKAQLKASILLALDGTTAVAEDMGRQIVTTGRRLSPEEVERVVGRITEKDVMEFAKKKLWDRDVAVSAVGQIEGLLDYNRIRNDMSRMLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3M9</accession>
    <name>A0A9W8Y3M9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3M9"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="155" mass="15387" checksum="3F131095DF260604" modified="2023-11-08" version="1">MILPPNKQAAAPTRPPPITTTLAAAAPVEADAAADPDLVALDDPDAAPEPAACVALAVVLLAPLPTLALPVAPAPPVNFSAPAVTTTNILATSLGSYVVVLLPGKFASLPPADSLHTAPTPPPRPAEGDTTQSWVAVKSLPGLGSDSSMCQVEGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y485</accession>
    <name>A0A9W8Y485_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">DNA repair protein rad16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RAD16</name>
        <name evidence="14" type="ORF">N0V83_007361</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y485"/>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF12">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="436"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="777"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="862"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="37"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="81"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="187"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="253"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="289"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="337"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="356"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4366835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1030" mass="116309" checksum="C0991053C115C6E4" modified="2023-11-08" version="1">MAPRRTRKSALASEIASATTTASINRPLEDLHMADIRHNQKRTRSSRAFVPNSSDNENSTIEVETPEQSALLRRVLKEVSIPKKKAMSTPGRPSARSNYAQKVEQESSNGSPSLENSDYETPGTSIATTPAASIRHGKLSTPSGLSKAVTTPPNTASARKRSRLAVLDSDEEDEIVDMDAELALQLQMEEDNEVAPQSKRQKIEESDDDIVVISSRKTYTGKGKGKAKVEEVRSDDDADSLLEELVTPPSYKGKGKAKAKAKAKAEHGQSSRGRPARASAKKKGRFTEEDFLDDADDSDVVEFRPDESDDLESLLNDLSTPAASDSEEEPLMVASARLKKAASKSKKTSRGKKKLSRAEQEEGERRRARFAHIEDKWERKRAMNRERAEQQHPKLITMWKDLEKIPVLEVQKAEQPKSISRRLKPFQLEGLSWMVRQEQTHYKGGLLGDEMGMGKTIQAVSLIMSDYPAKQPTLVCVPPVALMQWSNEIREYTDNKLKVLVYHGTNAKCKRMSIKELKSYDVIMVSYNSLESLHRKETKGWSRGEDIIKEASPLHAIHYHRLILDEAHSIKSRNTGVAKACFALTGTYKWCLSGTPVQNRIGEFFSLLRFLEVRPFADYFCRSCDCEKLHWAVDDDHMCVACNHGASEHISVFNQELLNPITGDDPELREEALTKLHMITARIMLRRMKRDHTNSMELPMKDIIIHNEFFSEVERDFSTSIMSNSSRKFDTYVAQGVMLNNYANIFGLIMQMRQVANHPDLLLKKNAAEGAGNVYVCNICDEPAEDAVRSHCRHEFCRACIKDFMDTCEASGTEADCPRCHIALSIDFEQPELEQDEDSVKKTSIINRIKMENWTSSTKIEMLVYDLYKLRSKKQTLKSIVFSQFTSMLQLIEWRLRRAGFNTVMLDGSMTPAQRQKSIDHFMTNPDVEVFLVSLKAGGVALNLTEASRVFIVDPWWNPAAEWQSADRCHRIGQKRPCVITRLCIEDSVESRMVALQEKKAAMIAGTVNNDKVAMDRLSPEDLQFLFRGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y488</accession>
    <name>A0A9W8Y488_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y488"/>
    <dbReference type="OrthoDB" id="426718at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021838">
        <property type="entry name" value="DUF3431"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37490:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37490">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11913">
        <property type="entry name" value="DUF3431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4365202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="294" mass="32951" checksum="5DF85F456C213F5C" modified="2023-11-08" version="1">MRGRIAIVAASTTILLVIIGLFISAPKSWRFGASPSSVAGHYPYLKPSPLPWNPPKPKDPSGDDPAARLIVKVKLEEEDPPSWIDSLFPTWRKEVVTIGLEFSQLHENGRRVDKGRIANAYLTWIIENYKNLPDTMVFLSPDKQSQGESRAGSDRKNDISNLQIPFIQSSGFANLQCPSPSTCADSILPFRSPPNEFRTLEVNMTKAWVGIFGNITVPEQLSTPPCAEFAVSKAQVQKRGVEEYLKCWQWLNRTIMDDDSAGLVFAYLWHVIFGRDAVFCPQEKQCECEVYGKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4C9</accession>
    <name>A0A9W8Y4C9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_007852</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4C9"/>
    <dbReference type="OrthoDB" id="421993at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF67">
        <property type="entry name" value="AMIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="3">
        <location>
            <begin position="153"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4366216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="590" mass="64272" checksum="C06ADC521B63A4BB" modified="2023-11-08" version="1">MSEGIRTRFINYPTPRAHDAPFQAPPPGSNPVIKGWPLSYLANLVAFVPILPSILWSNAGFGSLRKCDQLQGIEPRYDPTVIPLSQPGGEAPASYTDKKTLRTPPNTKSDARFHTIEDFHSAYASGALTPTDVVEALLPLIRRDVAQRSPYSTAFMESKVELIRQAAEASTQRWKNRQPLGILDGVPFGVKDDSEVTGYKRYIGTSHDYTQGGAKETSWCVKKVEEQGAILVGKLNMHELGMDTTNNNPIWGTPLNPYNNSYYTGGSSGGAASAVGHGLIPFAIGSDGGGSVRIPSNYCGLYGLKPSHSRVSTAPMPDAGKSVTVRGPLASSMCDLELSYRVLAQPDPSYYPSSLFSPPRELTGPRNKILGVYKSWFDRADPPVQEACQSALQYFKSELGYDIIDVSIPLLPQGQLAHAMTILAEGASGHKTSIYDLTAPNRILMSVARQTPATDFLLAQRVRNLLMEHLANLFRIHPGLVIVTPTTPNAGWPIDPAELSHGVTNGNMQIRNMEYVWLANFTGIPCIQFPVGYVDGFKGKGKVPVGLSGQGEWGSEDALIEFGYDGERWLHEGYKGGRIRPEGWVDVLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4G9</accession>
    <name>A0A9W8Y4G9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cation efflux protein transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_007485</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4G9"/>
    <dbReference type="OrthoDB" id="78296at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098771">
        <property type="term" value="P:inorganic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1350:FF:000012">
        <property type="entry name" value="Cation diffusion facilitator 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000005">
        <property type="entry name" value="Putative Cation diffusion facilitator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="entry name" value="Cation efflux protein, cytoplasmic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050291">
        <property type="entry name" value="CDF_Transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840:SF11">
        <property type="entry name" value="CATION DIFFUSION FACILITATOR 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="8">
        <location>
            <begin position="183"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="136"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4366955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="470" mass="52539" checksum="4CE955521C72BE39" modified="2023-11-08" version="1">MVGARRNLLSSNSSYGNNNVNGNGKGDDFDKYQRSDVESGYGTINPVPPRQKFRDAIEQTMKSVRAMDMKKKLIENVDHTALEIYRKSDESLKCIHNKKVRAFYEEQNKRLDDWLEVDMVVSSLADDIVDSMNPADPDHDGVAEDRGPLGTSGESLEPFLPEDERARRAKSAKHVRWAINTNVIVNILLLAAKGVAAIWSNSLSLIASLVDSALDLLCTIIIWSTNRLVRWRLSRLKKKFPIGRKRLEPLGILVFSIIMVISFLQILQESVKKLLPSGEHKVAELPPAAIFSMVATIVVKGTIWFGCARVQTTQVQALAQDCKTDVYFNTLSLLFPLIGHKLHVWWLDPVGATILSLFIIYDWAGTCLENVTRLTGEAANDRVERKMMYMAYRFAPLVEGFKSIKCYHAGDGVCVEIDVLMPEDAPLSKCHDVAETLQYCLEGLNEVDRAFVTMDYTSQGPTGHATSDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y536</accession>
    <name>A0A9W8Y536_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Chromosome disjunction protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Ribosomal RNA-processing protein 44</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">DIS3</name>
        <name evidence="19" type="ORF">N0V83_007216</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the RNR ribonuclease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y536"/>
    <dbReference type="OrthoDB" id="372421at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071034">
        <property type="term" value="P:CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071031">
        <property type="term" value="P:nuclear mRNA surveillance of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09862">
        <property type="entry name" value="PIN_Rrp44-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.690:FF:000005">
        <property type="entry name" value="Exosome complex exonuclease dis3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000010">
        <property type="entry name" value="Exosome complex exonuclease DIS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.700:FF:000001">
        <property type="entry name" value="Exosome complex exonuclease exoribonuclease (Rrp44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041505">
        <property type="entry name" value="Dis3_CSD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022966">
        <property type="entry name" value="RNase_II/R_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033771">
        <property type="entry name" value="Rrp44_CSD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033770">
        <property type="entry name" value="RRP44_S1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF35">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RRP44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17849">
        <property type="entry name" value="OB_Dis3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17216">
        <property type="entry name" value="Rrp44_CSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17215">
        <property type="entry name" value="Rrp44_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01175">
        <property type="entry name" value="RIBONUCLEASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="10" id="KW-0271">Exosome</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="PIN" evidence="17">
        <location>
            <begin position="87"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="RNB" evidence="18">
        <location>
            <begin position="523"/>
            <end position="856"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ4367631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="990" mass="111936" checksum="86B7C08553B0300C" modified="2023-11-08" version="1">MEYFSLKRSSAADRGQSNLSSKVYVRSTKSGKVQKIVRELYLRQDIPCSSNICRACLEIAPSDYKKKVAPFVLSDTPAGSKDFPNGQYLVPDTNAFLTGMDLFEVETAFHDVIVLQTVLEEVKNRSLPLYHRLIALTKNEGKRFYVFFNEFRQETYVSRDAGETINDRNDRAVRKAVQWYNHHIAEAVAARSKTQKRIPTVVMITDDRDNLRKAKAENVPSKTLADFVSGLENSDELLDMISAAQEQREVRSKKAEVFYPEHYSVSKMMTGVKSATLHQGIFNVSPYNYLEGSVHVPAFDKSLLILGRENSNRAVSGDVVVVEVLPQDQWKAPSTKIIEEETVNKNDNAEAEEGENVIPERERRALQEEVKRVHGQSAEGRPQPTARVVGVIKRNWRQYVGHIDRDSVRSTSKSSRQQQTVFLVPMDKRIPKIRIRTRQAGELLGQRILATIDSWDRDSRYPVGHFVRSLGELESKGAETEALLLEWDVQYKPFPKTVLDCLPAEGHDWKVPASLDDSGWKGRKDLRDLLVCSIDPVGCVDIDDALHAQQLPNGNFAVGVHIADVSHFVKPNNAMDKEASQRGTTVYLVDKRIDMLPMLLGTDLCSLKPYVERYAFSVLWEITPDADIVSSQFTKSVIRSREAFSYEAAQLRIDDKSQQDDLTKGMRTLLMLSKKLKQKRMDAGALNLSSPEVKVRSESETSDPVDVQTKKLLDTNSLVEEFMLLANISVAAKNYEAFPQTALLRRHAPPPKTNFEELDNQLKVKKGMELKTDSSKALADSLDKCVDPSDPFFNTLVRIMATRCMMSAEYFCAGTQAYPEFRHYGLASEIYTHFTSPIRRYADLEAHRQLAAAIEYEQLDPSLQSKAKLEAVCKNINVRHRNAQMAGRASIEYYVGQALKGKDITEEGFVMKIFSNGFVVFVPRFGIESLIRLRDLATPEPEADFDAENYKLSIKGDVKRSVELFEKVTVRITDELEESTGKRKVRLSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y576</accession>
    <name>A0A9W8Y576_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007276</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y576"/>
    <dbReference type="OrthoDB" id="5417895at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052973">
        <property type="entry name" value="Fungal_sec-metab_reg_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35392:SF2">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35392">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="259"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="643"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="643"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="659"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="778" mass="86571" checksum="24610B2A1414AEF4" modified="2023-11-08" version="1">MGRRPNQLILEYFERGPKLEDASNRYQHTCKSCGEKFPKGRIDSLTTHLVKKCPALPLRDRQRALLQLHEMPIPDHMADHMAQTTGATGPSQMQGGQKMSLPFAPSKQLSALETLAEVSRQHLDLSGKRMPEKQSLHGHNNQNHAGLLDEFLIQDDRPEGSDMVGMSQSMDMSNTPALPTMYQFNGPLHHSPTNSPHMGHMPLSSSGSMAQMVPSLVMAASAANELMPLTNGLTMEPELNMSGNGMGMSDRFFHVQGRGQWPNLQPSSLDPLLQDHSNQHLSPKDDHTKSMPHPRPIAMNPNTQAHFTTDFSMNQKPAKPKVRGRFSDSRRKEVQEVRKRGACIRCRMLKKPCSGDTPCNTCQNVESARLWKQPCIRTRIAEEFSLYSAGLHSVLSYHATNQAKGQIRLDQTPGRIEATHYPDSSIFVTFAPLKCQQAHAAQHSDIDPAIFGALVSPDLELIDSEDDVGGKLDLYIKKMAPHFFITEDSAFMKTTLNTASSMPSSNQDGLLSKALELWNLTRILTSDNLTWQLFSNPSLAPTMAPSTMSSSDVEDATRTSITALNHSKSYDLIKMQLMGATEKHAACLARIVMNDLERRLLQRQQADPFETFLVAVVLLACVERMCWLFRTWEVPPANAASTDADTQAASSTETDLASALQSASSPTVSTQDVGYQPRRNPRWPLDKSPASFSQQGERFSDILNMLLKMRGVPPKPVPRSTDGMLVMWADDVDEKVRDWYDGIAVTAKTLDDRASARFMGEEPREWELKYVGKIIRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5A3</accession>
    <name>A0A9W8Y5A3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006378</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5A3"/>
    <dbReference type="OrthoDB" id="2151417at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38849">
        <property type="entry name" value="SMALL SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38849:SF1">
        <property type="entry name" value="SMALL SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040979958" description="Small secreted protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="179"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="222" mass="22176" checksum="1D84A1AE19202DCB" modified="2023-11-08" version="1">MHFQSFTVLTLAALAIAAPVKRQSGPVLADTTYNDISIAGGVAGNAEAEAKAVFSALDLANPENIDPADLDFLGQVNDVANDAEVEAFNTAIDAATGDEADQIQNGKIKNKVLKLMATVIQLGAEQAQGDDTVASKLADETTKLNNNIAIDTKNAGQTAIAEPFDGTISGGGSVVASAGNANSNGTSTADATAAADASATATAAAPATATSKAAKKKASKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y630</accession>
    <name>A0A9W8Y630_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA polymerase delta subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_006460</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y630"/>
    <dbReference type="OrthoDB" id="514823at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904161">
        <property type="term" value="P:DNA synthesis involved in UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.20">
        <property type="entry name" value="DNA polymerase delta, p66 (Cdc27) subunit, wHTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019038">
        <property type="entry name" value="POLD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041913">
        <property type="entry name" value="POLD3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17598">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17598:SF13">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09507">
        <property type="entry name" value="CDC27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="157"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="377"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="209"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="269"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="391"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4368105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="417" mass="45934" checksum="8579056D9EE9108F" modified="2023-11-08" version="1">MLFDFHQKQNAKKPNAVHATYLVSGTKKSAEGTNIANGRDGEDTAMRSSPFMSSMPETEEHIEEHVKTTTIVLAREEELEKTRAEFDEETSIHIYSLESGPIEDLNVLSTCNHEIAKACAQEDSMERWRMYGSIHNPYIKRRTAKYALPVATSNLDVSDRHLPKPAAKANAAAQATESEQPAHVPKCSVPENTSGRPTSQPHTSTSALKRSDSKSSNKDKTAGDIFKSFAKSKLKPNGAERSQGSTPAPAADEPMQGMSEDEGDADDEPQIKADEEKNEVARKAREERAERLRKMMEDDDEDMPDAPNDIESQAEATPVGTPIAIEPAEGESTVTVQNGRRRGRRRIMKKKKVKDEDGYLGEQVSITKEEAVWESFSEDEPELKKVKHATTKSASTTKGKTAGKKGQGSIASFFKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6C1</accession>
    <name>A0A9W8Y6C1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_005971</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6C1"/>
    <dbReference type="OrthoDB" id="10262656at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17330">
        <property type="entry name" value="MFS_SLC46_TetA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF39">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G03860)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="382"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="412"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="453"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="523"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="656"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="681"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="60"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="294"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="549"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="304"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="549"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4368889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="749" mass="82027" checksum="21CE1A88837C6844" modified="2023-11-08" version="1">MSGRRPPIGSGRKSSTLHYQTFPTAPPKSRGRPLSTPSPDHEGGSEVDDQHHESPLPKQQLIVLAIIALAEQTALNSISPYLPEMTKTFPEVKEGQAGLYVGLIASSFALAQFTTNFFWGWLSDKIGRKPVIIVGTFLTMLCFLAFGFCKTLWQAVLVQALMGIVNGNSGVVSTCLGEITDRSNQSRAFTYLPVVYGIGGITGPIVGGVLVFYQNPLNKSRPNPYPYLLPNLFSALVLALDLVVCMIWLEESLEEAKSLPPLGKRLGNLFSWMWQFTSSTRPTYVRRFLGKRKSHHPHHLDGIHEEDEEDDASDASGESAPTLFPHTNGEELTKKEILNRDTILLLVTYFIFQLANISYNSLYPIFAEELPPTGRGLKPEAVGLSLSFAGAITIVFQVGIFGKLRGKLGNKIAYKTSLAFFVAAFALMPWVGYKDSKSYGGIGTGAGWLWFELGVVLVIKTIAGVGGLTAALLMITNSAPNHNVLGTLNGLAQTLSAAGRAAGPFVSGGLFTAATKIRPKGEALPFGIFAGISLLGFFMSLGIRGEGLEAEGWSDEEEEEDEEEEIGDEPQSTNERTGLIRNLAAPLVDSENRHWVILALQGPKSASFHGHKRDVDAEKHLKRLHKTTPLIESPLVGADHENLVFAQRHEANSIELFFDLFFVANLATFTAYHSITDYDYLLGYIGFFGILWSCWFQITLHDVRFARDSLYERVCKVTQFIVFVGLALVGSQFNPSSAKGEGYNTVSIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6P6</accession>
    <name>A0A9W8Y6P6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Checkpoint protein hus1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hus1</name>
        <name evidence="2" type="ORF">N0V83_005405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6P6"/>
    <dbReference type="OrthoDB" id="3801542at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007150">
        <property type="entry name" value="HUS1/Mec3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38166:SF1">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38166">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04005">
        <property type="entry name" value="Hus1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="636" mass="71204" checksum="ECA995E2F38B2504" modified="2023-11-08" version="1">MHGCLKMSIKSDAMSISSVWTDLSNPELDPSQVAGGENGIAEHPSTRMKQLGSADGRSEEGWALVRIDGRDWGKVMSNLRTKYLLPLRDIMDLLPTTSGAQIVQFGTNHWKCSNCKEGVIYSRTTGCVTNKRIWKCCRCGFADNSVLGDDACSFCYGTTESNQLYTPNDILLRHDFVDVFASFQPGAGDPGASSYIFCPLEPAISSPNTDLVDDWAAKVHQNLLRFDRFEESNEFGTLVPADDVQDDQQFEAILSISDPRAAPDVFLAEPANYGRLPAIQDTSCDRGHHHERNGSQLRPEAEAGADALARRAAYDEGYGTLSLDTTEKPQLSKRQHDELEEVNPREEDGQNDRSAEGRKKRKRLLESSQHRLACPFYKRSPSRCKYKSCARPGFSEIRYLNQHLERDHLYHQCERCGSTFCGIVGAEALASHRRVPNPCQWQPTIPSFWGIDQKLLDLLKRRKRVRGKSDEQRWLEIYSLVISDAADFDLPTACRSRFDSAWNSGTNGLDVDEKASVVEEYAYDADPARSQQQAIDTYSQELLRVLPDRLTSSVMGATQDSTIIEIWNSFARDKLYDVLQTALHGITSMSRNEDQIPVPIIERTLDLGLDLQTSMSSTDSVLEAAISLDFAQLDCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6T1</accession>
    <name>A0A9W8Y6T1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Isocitrate lyase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ICL2</name>
        <name evidence="9" type="ORF">N0V83_006213</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate</text>
            <dbReference type="Rhea" id="RHEA:16809"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57429"/>
            <dbReference type="EC" id="4.1.3.30"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Can also use Mn(2+) ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6T1"/>
    <dbReference type="OrthoDB" id="4078635at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004451">
        <property type="term" value="F:isocitrate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046421">
        <property type="term" value="F:methylisocitrate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019629">
        <property type="term" value="P:propionate catabolic process, 2-methylcitrate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.850:FF:000001">
        <property type="entry name" value="Isocitrate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006254">
        <property type="entry name" value="Isocitrate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018523">
        <property type="entry name" value="Isocitrate_lyase_ph_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01346">
        <property type="entry name" value="isocit_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21631">
        <property type="entry name" value="ISOCITRATE LYASE/MALATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21631:SF13">
        <property type="entry name" value="MITOCHONDRIAL 2-METHYLISOCITRATE LYASE ICL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00463">
        <property type="entry name" value="ICL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001362">
        <property type="entry name" value="Isocit_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00161">
        <property type="entry name" value="ISOCITRATE_LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="160"/>
            <end position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="270"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="486"/>
            <end position="490"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="521"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4369129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="603" mass="66595" checksum="E35B0D3FFCFD54DE" modified="2023-11-08" version="1">MLRTVSRSVARQSRSLRLSNRTPQLLRPLTTTAYRMSNSTLQAVDPPVSSALPGDSFQLLSEASKAGQAEDALFDAQVEAVKDWWASPRYKGIKRPYSAEDVVSKRGALQQSYPSSLMARKLFNLLEERAAEGKPVHTMGAIDPIQMTQQAPNQEVLYISGWACSSVLTTTNEVSADFGDYPYNTVPNQVQRLFKAQQLHDRKNWDTRRKLSPEERVKTPYLDYMRPIIADGDTGHGGLSAVIKLAKLFAENGAAGVHFEDQLHGGKKCGHLAGKVLVPVGDHINRLVAARFQWDTMGCENLVIARTDSESGKLLSSAVDVRDHEFIKGVTEETDPLAETLQEMEAAGAPGKEIDAYEAAWVKKHQLVTFDEAVVQHLEKEGAPSSTTSQYLSESKSNPNLSLLKRRALAAKYTKSPVYFNWDIPRTREGFYHYQAGVAAATKRGKEYAPYADLLWVETGDPNVDEAAKFARAIRADHPGKKFVYNLSPSFNWMGHGFTEEKLKSFVWDLAKEGFVFQLISLAGIHSTATITCELSRAFKDEGMLAYVRLVQAREKELGCDVLTHQKWSGAGYIDGILGAIQSGSSGSKSMGEGNTETGTFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6W0</accession>
    <name>A0A9W8Y6W0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_005823</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6W0"/>
    <dbReference type="OrthoDB" id="5296287at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF59">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="364"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="402"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="432"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="32"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4368741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="541" mass="59677" checksum="CD54BF27DE7C9B88" modified="2023-11-08" version="1">MGFLSDSTLAKYGRAIKHSPRELIFNRRLLVSAALYAMAGIPICWDQGSSSVVPSLPGFQHAFNITSGSNPGQISNFVSFIYITAGIGAFLSYFINDRIGRRWSLRLYMGIWVVGQLIATFSYGNLGALYFARLVSGAGIGPLTAVGPMSIVEIAPYEIRGVLTTWFSVIMLLSLTCASFTTYAVFLHTTSGHIQYQIVFFVPCIIIAIIFVASFFCMEESPRWLMLKGREEDAIKSLVALRGLPIDHPRVASEFQEIRAQIHEEHNKFGDASGGGFKAVLRETFLVKSNLRRVFQACISYALAQLSGANSVTTYLVSILTLMGVGGSTERNMFLTGMYSMSKFFYTLIASFFFIDALGRRKSLFVGITVQMISDLYIGVYVKYKQAGSIATGSSEAAIAAIYIHGFGYAVGLLVLPYVFVAELWPNNIRSFGACLTQAWHWLFFFGINKGTASMLSSMDNWGAFIFFAGWCFISLLYVWSSVPETAGLSLEQLDALFEGPFWQTNAQAKKQRQLANVIDSAETGSVENINEPVSKDSIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8V7</accession>
    <name>A0A9W8Y8V7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CBM6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_005271</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8V7"/>
    <dbReference type="OrthoDB" id="9970295at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04081">
        <property type="entry name" value="CBM35_galactosidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18821">
        <property type="entry name" value="GH43_Pc3Gal43A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005084">
        <property type="entry name" value="CBM6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006710">
        <property type="entry name" value="Glyco_hydro_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22925">
        <property type="entry name" value="GLYCOSYL HYDROLASE 43 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22925:SF3">
        <property type="entry name" value="GLYCOSYL HYDROLASE FAMILY PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04616">
        <property type="entry name" value="Glyco_hydro_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51175">
        <property type="entry name" value="CBM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040888636" description="CBM6 domain-containing protein" evidence="5">
        <location>
            <begin position="19"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="CBM6" evidence="6">
        <location>
            <begin position="321"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4370750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="445" mass="48016" checksum="8B82C11266FE1D4D" modified="2023-11-08" version="1">MKTFGLAILGLLASVADATLQIVPGATWTATNTGQHIQAHGGGMLKVGEKWYWVGEDKTNGTAFTNVNCYSSTNLVEWQFEGALLSRTASGDTGPSRVIERPKVIYNKKTNKYVLWMHIDSSNYGEAKIGVATGDTVCGKYTYLRSERPLGFESRDSGTFVDDDGKAYLLTEDRQHGTRIDSLSDDYLTVQKNVYVWKEKYEAPAIAKKNGVYFMFASQLSGWDPNDNYYSTATSLSGPWSGWKKFADSGSKTYSSQTTFIVPVGDQFMYMGDRWVSSNLMRSTYIWLPLKLDGTTATMKNSINWVPDASSGSMTAGPSENSYEGESATLSGGAKSVSCSGCSGSKAAGYVGGSTSGAVLFSNAQSSATTRTTLRIKHMNGDSSQRFADVTVNGKTQKVAFLPNGGSDPGSSVVHVDLKQGSNEVRIVTSGTWGPDVDRVLVPQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y969</accession>
    <name>A0A9W8Y969_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y969"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="11"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="69" mass="7609" checksum="5D176B6FC1B37856" modified="2023-11-08" version="1">MVLRRTALDLLRDNPDDLGLSQHETLPGKREQMESEKNTAENDGKDAMSSPQDEVHATNGGKEESGRCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9D0</accession>
    <name>A0A9W8Y9D0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flavin-containing monooxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004863</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9D0"/>
    <dbReference type="OrthoDB" id="74360at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050982">
        <property type="entry name" value="Auxin_biosynth/cation_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539">
        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-LIKE PROTEIN (AFU_ORTHOLOGUE AFUA_4G09220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539:SF68">
        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-LIKE PROTEIN (AFU_ORTHOLOGUE AFUA_4G09220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13738">
        <property type="entry name" value="Pyr_redox_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="672"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="695" mass="77166" checksum="4342D305C3FACBF9" modified="2023-11-08" version="1">MSQKPSSAWNDDPIRPHNADATIGWAGVPLSQTLGYYGHIARPTLPTNSELGFTPDYASVEVQKIVQSFLDSFAKNVEAQDVDAIIGSIYKDGYWKDVELLTWDIRALHGHEAIRQMLRERLSKTGIKNIRLNDQASPGIEKIGEDLSFILFHFEFDFAHGTGVGVARLSPFAAKTGSTAELADEKSWKIYTIGTALETVEGWDAEYGDKIRYQHGKIHDPLGKGRTYQELRDDELEGNDGFGPTVVIVGAGHTGLCMAARLKVLGISHLIIEKEDRAGYSWASRYASLSLHGPTYANHLPCLPFPHWFPTFLPAQQLAKFLQQYATIMDLNIWTNSQVDGKAAAYDEKEGRWNISITRGDGSKKILHPRHFIISTGISGTLPNVPEVPGMAEFKKNGGLVVHSSRHKTSPEWKGKRCIIVGAATSAHDLAYELSENGCQVSMIQRSATHVMSVEPSVRSLFSAREKTNRREGQPQNIVDQATFLKQPYPVEYELLPRGQQIARELDHNLLESLKKVGYRLHDGYHGGGAYSMFLFDQGGFYWDTGACKLIADRKIGLIHSEIDHFTANGVIFKDGSSQEADAVVFATGYMNTKSAIQALMGDEMAKKCHERWEKGNAFFLGPEGESIINHCPLPQKGLYSMFHQFSFTRFHSARLALRIKAEELGIDVTPYRNKPLGPPTTAAGRQPRPEGVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9X8</accession>
    <name>A0A9W8Y9X8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_005025</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9X8"/>
    <dbReference type="OrthoDB" id="10029320at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11051">
        <property type="entry name" value="CYP59-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF50">
        <property type="entry name" value="BIFUNCTIONAL ALBAFLAVENONE MONOOXYGENASE_TERPENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4370504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="561" mass="63024" checksum="5B6347DD8E6A1962" modified="2023-11-08" version="1">MTNPSDFPEALQFSGFRFADPQLVKDLENGSNTFNVAMQKKPTKLTDVDMTYHVWGTGRMACNLNYIMESIWKSNGRPEFLFLDVRPAQYPVALITSHELAEQVTKATKTQQYSVTKSPTIQEGFGQLMGKYSLLTEGGESWKGLRKTFNPGFAPQHLLTLLPVVVEKTYTFMKKLDALAKSSTVTEMESYCTNVTFDIIGEVVTNIDCKAQDETVSGNDIVRNFRMLGATYAGESGLAFEWLNVRKRITRIIYSQRLDVAVKNCIKDKFTEINASPGTTTKSAKDRSVLALALRDIDDLTPHLLQSIADQVKTFLFAGHDTTSILLQRLFYALSIHPQCLAKIRAEHDDIFGTSDPKEIFLARPDETMKALVYTSACIKEALRLWPPASSARMSHNGFKIRTSDGDEVCLDECVMYLCHYIIQRDRKVYGDTADDFVPERWIGDIDTSSATTDDNGSLTGSSKIPISAWRPFERGPRNCIGQELANMEARVILACVMRRYDFVKVGLGEVETDEKGKPIVDEKGKYKTKSELLSTMSITSKPMDKTVMRIKMHERDGRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA72</accession>
    <name>A0A9W8YA72_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005175</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA72"/>
    <dbReference type="OrthoDB" id="2419400at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07389">
        <property type="entry name" value="MPP_PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000028">
        <property type="entry name" value="WW domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.70">
        <property type="entry name" value="PhoD-like phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018946">
        <property type="entry name" value="PhoD-like_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038607">
        <property type="entry name" value="PhoD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043904">
        <property type="entry name" value="PhoD_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46689">
        <property type="entry name" value="MEMBRANE PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46689:SF2">
        <property type="entry name" value="WW DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G06520)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19050">
        <property type="entry name" value="PhoD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="489"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="315"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1212" mass="131900" checksum="02C1D7081F056ECA" modified="2023-11-08" version="1">MDGRKQGRPDEELFLRPSENADTPTIGPLRIRKDRDGRSPPPRTDSAGSSNQPAFSRPPPMPSFPAPPTSPPTAPLPYPDDRPPKQPTLGQGRYTPLGERERQTKNATPPEADGRRRGSNATGPSPTSPGQRARFDANDPNRPSVSGYGARNPDGSVQPSRLAERRGTAPKPLPDSPGPETPDKEGLFQKAPQRQGSSDVNASNPYPEYHQQYFPPPAGPKHDEPVLKIPNPGGVNRLSSTASTSTVRAQRGSPPPPETPIIPPPSGGIEARFAAAGIAGTSTLTNLQAQSAQNAAAAQRNQVYANQQPRPGLTSPAPSQQQQPARRPWTPTEQPGSTPHGPPTVYQGMDQVPPSSSPPQGPLPQAPTFSPPPTNVPPEHPLNQEMGRMNMNEEPPPAYSSITHPPTTQNQSYPNEKGQQPAAQGVSLSDPNAGHPAFANDPRQQAGPSQQPAAVVSPTPTANQFPPQIQTQLAQTPSPAPGPGPASPPPLPEGWIAHLDPTSGQYYYIHLPTQSTQWEFPKGPTPLNLNEPLSPTGTFVGGPLASPSFGKAPLASPGFPTQTASYAGDFGMAPLATPTAAGFTGPPPTAGVDQYKVAPTNGVYFGPYLRYTNMDIERGLWLGSILLITNTPHPPTIHIHQSADLSPNPRQLKANPIYTHQTWIFYRYDIDLRMDEDHAAKWTYAITSHLGCTRFEFLVAGRHETSWRFIAHSGNDFALNVNANERTKLGGVGLMWKDILQKNAECGGFHVQLGLGGQIYADRLWKELPLLKQWTATSGKENRKNAPWTAKHEEDVCHAYFHYYTSHFDQPHLREAFAQIPHILALDDHDIFDGFGSYPEYMQFSNMFKNIGRLGIEMYLLFQHHTTLEILRNVSNDMDLWTVTGTGWHFIKYLGPCVTVVGPDCRSERNPHQVMAGPTYQGIFPKVATLPPSVQHCIWMIPVPLVYPRLESVEHLASSMATGKKAVTGTFNMVGKLTSSVAGVVGAKSIVGDGFSSVKKAIGKSGLMSGVLSPFGDIDLLDELRDQWTHDSKDLERTYLIRTLQGIAHNKSLRMTFFSGAVDCCGAGLVHDPSRPQDHKTMYQIISSSVVNGPPGNYVLRLLHNNRALYVPQNGHRSNQQATDTKEDMMEIFTQDVNGQPREYKRLMGRRNYVACVIYDPEIVNGTFGQAVPGHGSGKLSLAVDFMVQNDGGYSAPMKYGPVIVPSLEYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAB1</accession>
    <name>A0A9W8YAB1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTH araC/xylS-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_004012</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAB1"/>
    <dbReference type="OrthoDB" id="2447880at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.10.10">
        <property type="entry name" value="DNA Methylphosphotriester Repair Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035451">
        <property type="entry name" value="Ada-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004026">
        <property type="entry name" value="Ada_DNA_repair_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02805">
        <property type="entry name" value="Ada_Zn_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00165">
        <property type="entry name" value="HTH_AraC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57884">
        <property type="entry name" value="Ada DNA repair protein, N-terminal domain (N-Ada 10)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="6">
        <location>
            <begin position="84"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4372238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="274" mass="30074" checksum="35F5F7FBF323A141" modified="2023-11-08" version="1">MAFTTDADRWRALVTRDANANGHFIYSVRSTNIYCRPTCPGRLARRANVGFYKTAFEAEAAGFRACKRCKPNTILEDPQERAVEKACLLIDESLRSSSPTLKLQDLAKKVGLTPRYFHKIFKDKTGLTPKEYTTKAKTENHYSPSTVAISTGSEASIQTPDWETFDFNDLIDYDADLNATWTEDIGTEALNARTTGCDTEIDMDMLLRSWSSGYGPSGIDPGLGSTDDILLRSLEAMSAPNSLQGAKAAPSVSTFELDAAALLRCDSVADLIQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAU4</accession>
    <name>A0A9W8YAU4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_004891</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAU4"/>
    <dbReference type="OrthoDB" id="256333at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="7">
        <location>
            <begin position="8"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4370373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="315" mass="32917" checksum="3C658D4D227574F7" modified="2023-11-08" version="1">MVQKGVMGTSLPCTGSHEGAGTVVAIGSSVTSFAPGDRVMAGIIYHPCGTCADCLGPENYRQYCQFSGGYCGVSTHGFFAEYARIDASQAARIPDSVSFETAAPLACAGCTIYRGLVLSAVQQGEWLAITGSGGGLGHLGVRFAKALGINVIGIDARDQGLELTTLGGADVTVDARRGLEDVVDKVHRVTNGQGVKITLNVSDADSAMATSCAITQMHGTVIQIAQPSLVSIPFQELVFRDIRVRGSLIASPEEARSMLEVVAKHGISVRTNVFEGLGDLERLVELAESGKMMGKGIIVLDRAQIGVGKESGKWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAW7</accession>
    <name>A0A9W8YAW7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003299</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAW7"/>
    <dbReference type="OrthoDB" id="10250730at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33930">
        <property type="entry name" value="ALKYL HYDROPEROXIDE REDUCTASE AHPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33930:SF2">
        <property type="entry name" value="BLR3452 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxymuconolactone decarboxylase-like" evidence="2">
        <location>
            <begin position="222"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxymuconolactone decarboxylase-like" evidence="2">
        <location>
            <begin position="354"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="447" mass="48856" checksum="2AEA382AB70BDBAD" modified="2023-11-08" version="1">MTTDPDVTKSLARGGLTPADIDYVIYSHVHWDHVGEPRDFDTSTFVVGHGACALLGGTSSALRGGHSFFEPDLLPEGRTIELSNPCTNTLQKRDSATSLDAVDFNGPWQSHGTLPETLDIFNDGSLLIVNAPGHLPGHINLLARTSENHEPVLPNPDIESPVEIKTHHDALVKPIPPAHNFLKLPPNMSLTPEQQALKERFNAELGPAQFDESWSRLLKHSPEMFAASLRLTSVPKKKGHLSPKIQSLVSLAVSAASTYLHVPSIHRHTRAALANGATKAEIVEVLCLTSTLGIHAGTTGIPILCEVLEEQGKGIPKGLDGMNKEQLGLKEDFEKKRGYWNSMWEEFLRLSPEFFVAYTELSSVPWTNEGGKGVLEPKVKELIYCAFDTAATHMYTPGLKLHMRNVLNYGGTAEEIMEVLELASLLSISTMDVGLEVLDKELSGQSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAX7</accession>
    <name>A0A9W8YAX7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein BIG1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_005117</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BIG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAX7"/>
    <dbReference type="OrthoDB" id="9985059at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037654">
        <property type="entry name" value="Big1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046756">
        <property type="entry name" value="VAS1/VOA1_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28285">
        <property type="entry name" value="PROTEIN BIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28285:SF1">
        <property type="entry name" value="PROTEIN BIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20520">
        <property type="entry name" value="Ac45-VOA1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040762896" description="Protein BIG1" evidence="11">
        <location>
            <begin position="20"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="V-type proton ATPase subunit S1/VOA1 transmembrane" evidence="12">
        <location>
            <begin position="239"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4370596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="288" mass="31726" checksum="8610E16F9CD5BD5B" modified="2023-11-08" version="1">MAKALVGALALAALPSALAFRNTSPFFLFSTAELLIDTTDTSVSESSKVTADVLGALKDCPTRSYVIVEQEGVSFNDYLDGYTTPQLRQYMGGKHEEVKTTFAINEVVGQVDAAAVVSHLQAKCAPSSNDQHDAVWFRRIKAQAPSAAKALRTQQLHSEDATLEQHIVNDQKSRDYTVIYITSPQTESQAKAQLADEQYSYEMESSFDQSVQMELKRDLSSHIKRANSTAEGGLFERYQYFTPGLFMGLAAIVPLFLILLVGIRALTSLEVSYFAFSKEMGPQGQKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBU5</accession>
    <name>A0A9W8YBU5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBU5"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="99" mass="9745" checksum="7CAF1E387B99F758" modified="2023-11-08" version="1">MKFSLITFMAAGGVPFPTGGFSGFPSGVPLPTGGFPRPTGPRPSGFPSGVPFPTGGFSGFPSGVPLPTGGFPRPTGPLPSGFPTGLPGFPSGGFSGFRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCI5</accession>
    <name>A0A9W8YCI5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein SDA1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SDA1</name>
        <name evidence="11" type="ORF">N0V83_003580</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for 60S pre-ribosomal subunits export to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the SDA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCI5"/>
    <dbReference type="OrthoDB" id="2196187at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027312">
        <property type="entry name" value="Sda1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048292">
        <property type="entry name" value="SDA1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007949">
        <property type="entry name" value="SDA1_MD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012977">
        <property type="entry name" value="SDA1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12730">
        <property type="entry name" value="HSDA/SDA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12730:SF0">
        <property type="entry name" value="PROTEIN SDA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21638">
        <property type="entry name" value="SDA1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05285">
        <property type="entry name" value="SDA1_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08158">
        <property type="entry name" value="SDA1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SDA1 N-terminal" evidence="9">
        <location>
            <begin position="67"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="SDA1 middle" evidence="8">
        <location>
            <begin position="546"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="domain" description="SDA1 C-terminal" evidence="10">
        <location>
            <begin position="702"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="515"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="678"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="515"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="551"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="589"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="696"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="742"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4373286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="755" mass="84699" checksum="0EB434C36034ABA5" modified="2023-11-08" version="1">MKRKVGALEKIEADHAALRYKIKRDPQSYRDDFSNQYQQYETLRDLFLQSPGTTDTGIVALRDLIEFVSHVADCYPDLTSRFPEDLTQILLKHHEVLEYELRDKIVGSLVLLKNKDVIDSTTLLNTLFPILITTPSKSLRQLLFTKILSDLRAANAKTTNHKLNRTIQTVLYNLLESDKESPKGIWAVKITRELWKRQVWTDARAVEVMRLAALSDNEKVITGGVRFFLGGDKEREDAAEESSDDDNDVDMAKLRHQVGINKKSKKKERDLRKAAVSLKKKEKKKNAPHPLNFSALHLLHDPQGFAETLFSKHVQNSKSKLALESRLLVLQLVSRLVGLHKLTVLSLYSYFLKHLTPRQASVTSYLACLAQATHNYVPPDVLEPLVQKIANEFVSEAAASEVAAAGLNAIREICARQPLAMTDTLLQDLVQYRKSKDKGTMMAAKGLLSLYREVGAELLHKRDRGKDAAMGIRAGTIKERRYGEEAVGEIEGIELLEKWKEEERRKKMEAAGLDPDVDVDIEEDEAEDWKKWDVESDEDSDDSGGWINVESDGEDIEISDSEDEKAKSKQPPAKKAKLSSATPAPTTASDEKENSKSVEPEAAKSITKLLTTTILTPADLKQLQALRQTTTITSSLQSSKRAQLLAARHADEAITAETIELAASIGKKNTKEEKIAMAKADRETNHQSTTAKRKEKKEAEGKSTTNKEKARKKNFMMTLGKAKSKNKRSLTDVKRTLQGHIDRKKRGGKRGNKGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD13</accession>
    <name>A0A9W8YD13_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamine amidotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003836</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD13"/>
    <dbReference type="OrthoDB" id="92161at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044992">
        <property type="entry name" value="ChyE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695:SF4">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="1">
        <location>
            <begin position="98"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="286" mass="32112" checksum="211AAD39C28DDD63" modified="2023-11-08" version="1">MAPPKSRIIVSDHPDKVRMLVLETDVTHPDTGIEKGSFGVVLNELLKSAGEEHEPSLGIETVMQYVVEPEGGVVPKPEEIGDDIHAILITGSCWDAHGDDAWIHKLMGFIREIWTTRPDIRFTGICFGHQILCRTLGSTVQPEPNGEWELSHSPIMLSDIGRSLFNLPSTEERIHLHQMHLDHVVSPPSPSITDLLPEGTKVHVWGKSDHTGVQGVYIHKRLFTTQGHMELNEVMVKRQLETRVEAGSIKEDQKDEAEEKADWKHDGLLVAKAVLRFFHGDDDKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD71</accession>
    <name>A0A9W8YD71_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001953</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD71"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="135" mass="15023" checksum="00AD0845C4E1F73C" modified="2023-11-08" version="1">MSKQYQNKLHPDIDGNSFSGRFTTISTVTTYNDVIVDDMFDQPVLAVGGDDASWPDVPPWQLPDECFEQQQQQQQQNQPEDAGETLLPPSPQEKEGDDAPKQKGKRKSEGEVEGSKAKKKATSKNSDDEGERLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD89</accession>
    <name>A0A9W8YD89_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ADF-H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_001983</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the actin-binding proteins ADF family. Coactosin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD89"/>
    <dbReference type="OrthoDB" id="20822at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11282">
        <property type="entry name" value="ADF_coactosin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000018">
        <property type="entry name" value="Coactosin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829:SF56">
        <property type="entry name" value="ADF-H DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829">
        <property type="entry name" value="CORTACTIN AND DREBRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="6">
        <location>
            <begin position="5"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4374905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="151" mass="16840" checksum="A401F2F0314592A2" modified="2023-11-08" version="1">MPAPSDILSDPDISQAYEDVRSDKSDTTWLILKYASATSDSLTLAAKGTGDIAEMTESLGDEAAYAYVRMKLGNDEYSERVKFAFVIWQGPNTKVMRKAKMSFQSGQVKQVIRTYAVEIQTSDKKELEEEAVTLKLRKAMGANYDRQGSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDK2</accession>
    <name>A0A9W8YDK2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002031</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDK2"/>
    <dbReference type="OrthoDB" id="5382170at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054293">
        <property type="entry name" value="DUF7029"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055647">
        <property type="entry name" value="DUF7223"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22974">
        <property type="entry name" value="DUF7029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23865">
        <property type="entry name" value="DUF7223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7029" evidence="1">
        <location>
            <begin position="32"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7223" evidence="2">
        <location>
            <begin position="205"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="470" mass="50820" checksum="7CCD91D4DCFA20B1" modified="2023-11-08" version="1">MRITKKFESELAYVENENGWNSRSTFASQVKVSSQKPVLTLEEIEHHLQDVECGDATMKLHFVDTSSARDARSACNGAAGGLIITSHETCNQDGERAVYRVHDVAFAEDGDALELAVSRASWQDAFDTFDISFGHTTENHLYRRHADFAKVRRKRQAQASQIDIPADTPDSKVTATFNLTSSLLDTTFSAPSFLAGLDQLVPIPDLPIEIGCKVCTTSGRLELTQGAIKIDASQIDLIPDVLEGGDDGKEITSVITGGFMELAATGMSAHLELFARPKVSGAYEIALFSIPILGFVVPGVGKAGAVFEPRIAASFDISGGFEMNYGIDVAVPDNSNLRIELTDIKKSGVNGFPGSTLTPLPLSVNVTNVDILLGLAFKPSIPIGFEFTDKLKADVTISMNLPRLDALLSTNVAGNCGNQTGSNVTLPNAPDFNYGTVQHYINNSSGYYNMFQHINANHNYVCNTCAYYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDL1</accession>
    <name>A0A9W8YDL1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_002892</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDL1"/>
    <dbReference type="OrthoDB" id="40579at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47660:SF2">
        <property type="entry name" value="TRANSCRIPTION FACTOR WITH C2H2 AND ZN(2)-CYS(6) DNA BINDING DOMAIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47660">
        <property type="entry name" value="TRANSCRIPTION FACTOR WITH C2H2 AND ZN(2)-CYS(6) DNA BINDING DOMAIN (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="65"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="122"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4374151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="873" mass="96841" checksum="10D3C6C31188A283" modified="2023-11-08" version="1">MFVQSAQRDLLECRGPLALLHTSASDTFLTSQRDLLKRHVTNHDSKSANKRQKREIARDTRAVQACEACSQSHLRCEDAKPCSRCKKKNIICRVPENAVAEDYDVNELDAVHAAQDLLDLSNRFDYPSSQPGPDAGVDYSSTPRSGAQATSDHFDLSSSLSTAHTANMATRTPNIPNGERCLTASNDMLPPSISDGGSTAVSQAYVDDPTTSMSFFDNPIAHFDEMPEHDPFLPDYLRNMPPFEAFLSGQATPRGIMDLSFDLDVGLTDFDLGLLDQYNFEVPFSADTPSDAQGVEQLVPDNDSVPVRTEAFKQSIWRYLPQPHKDYSRAEQANLAFSEPDKDGNRRPQITQRRVIAEKLHRVSRDQLMALVLGTCSPENVRRIASAFPSLELLDGLIQYFLTSPALDAQSWFHLPTFSPSKIGPELLACIVSAGAATTPDVPLRKLGFAMHEASRIGQAKKFESDNTSIRDLENIRNLFLQLKVGMWSGISRKMEISESFLQPLLTMLRRGGRFRGLTWKEISPNTEDQGSVLEDKWQAWIHQESYLRVVHRAFELDRQSSMALLKPPLISYSEMQLPLPSSNNLWQAKSAVTWKAAYLDTASTAAKRPSAIDCLLDLKHLSQHDHASATYLYMTWGMVWEYHSMNALSVKSHAKANSNSLILSSRYQELTKQLDDFRVGSPPLSKSLEVTLELMFVHLNAPLDDIQLFAGIEGQEEARGAYPVLRDWTKSVSARQALWHAGQILRAAEGLPKALLCNFNAIAVYHAGLILWGYGFIKRSSATETTPAEIPQTPVVLNGDDFLSSRRFITLNRGLPSIRSPGSQATTSLNDVPGVMDVLIQLLRATHGAVEACPPLVGNLVHLLEGLRAATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDR2</accession>
    <name>A0A9W8YDR2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">deoxyribose-phosphate aldolase</fullName>
            <ecNumber evidence="2">4.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">2-deoxy-D-ribose 5-phosphate aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_002989</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2-deoxy-D-ribose 5-phosphate = D-glyceraldehyde 3-phosphate + acetaldehyde</text>
            <dbReference type="Rhea" id="RHEA:12821"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
            <dbReference type="EC" id="4.1.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeoC/FbaB aldolase family. DeoC type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.4" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDR2"/>
    <dbReference type="OrthoDB" id="70823at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004139">
        <property type="term" value="F:deoxyribose-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009264">
        <property type="term" value="P:deoxyribonucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00959">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00114">
        <property type="entry name" value="DeoC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011343">
        <property type="entry name" value="DeoC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028581">
        <property type="entry name" value="DeoC_typeI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889">
        <property type="entry name" value="DEOXYRIBOSE-PHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889:SF1">
        <property type="entry name" value="DEOXYRIBOSE-PHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0704">Schiff base</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="359"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4374248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="389" mass="41719" checksum="CB6CC1025E9D522D" modified="2023-11-08" version="1">MSTPASDPHPIPLTESEVLIRGWLAANPALDASLGPVELEMTDRYTNEEWKRQIEETVRNVTREVDSGERAAKVYEGPRMRNGDEALKELARTVDHTVLKLDATSAQIDTLCSEARTEGFKSVCVRLNWVQRCVSNLKGSSVLVACVVGFHEGTQDTHSKLREARAAVLAGASELDVVLNHHILTQHNKPSFIPDKILSSKDSAIDSITAANTVRNKLSSRDGPGGSDSDVPDYSAIYTELASLRSLCPAPVTLKLILETSQLTHPQILAASHLAAAASFDFIKTATGFNGHGATLPHVQLMVAAAEYLATQGVGRKKMEVKASGGVRGLDSAVTMLEAGATRLGTSSGLWIMQEARKKVGEEKGSGSGEESERPGGGITRLYTDDSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE92</accession>
    <name>A0A9W8YE92_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PQ loop repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001931</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE92"/>
    <dbReference type="OrthoDB" id="407617at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="26289" checksum="1E39667CA950CDE4" modified="2023-11-08" version="1">MAQLSNEEHRRIAGCYDVFVVIVWGSIWRCMVYSRKWRPWTATLVLVVLMTIFAAVEAGLIFAVRPPYQRGLEWPILLIGIIAFLTLIAGYIPIPFELLKRRGRVVGIDFVFLAIDWCGAFFSLMALVAQNEFDVLFGTMYALCCAIEMSMVVSQLIWLFRTRGIRKRAKADGKTFDESNEGIEWQSKGIDLETKFLKLVSRKSLPEEDFETEIENPDRITPKTVPNAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YED0</accession>
    <name>A0A9W8YED0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_002208</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001433">
        <property type="entry name" value="ABC transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:006099">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF119">
        <property type="entry name" value="ROA1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="429"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="520"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="544"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="574"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="660"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1064"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1096"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1137"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1174"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1206"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1291"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="79"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="716"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1326"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1332"/>
            <end position="1349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4375124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1349" mass="148867" checksum="A9CDBDE43DF47001" modified="2023-11-08" version="1">MESPEERQTQAGLVEPDDKHGSEVAGPPRLEDQDQDRTEELVTEARKPTPTASIQQFATNTSHDNQNAISLREVNPVSVRLEHLSVSVNESPNAFARAFSKRKTPPSHSHVKTILDDISADMPCGTLTAIIGGSGSGKTSLLNQMSGRMKGKRLGTSGRTLFNGSQDASRIRSAYVIQQDILLPTLTVRETLKYAAQLRLPSSVSEAGRIQLVEEVILELGLKEAADTRIGNHAHKGCSGGEKRRTSIGVQLLSNPSLLYLDEPTTGLDSTSAYQVVRSLQTLARKGRTIIVTIHQPRSEIWNLFDNVILLTKGNPAYAGSAKDCLPYFAKLGHEMPPFTNPAEYLIDVVSVDNRSEEAEGAARERVDHIVEAWRAHCSIAANEKGEPGLTAPALAKTPSTLESTHTALVQQIRVLTARTWTVTIRDPMGMFGSIVEAIGMAVITGWIFLQLDGSLSGIRSRQGALYNAAALQGYLILLYETYRLTTDIQLFDEEARQGVVSIPAFLISRRLARIFIEDIPVPLIFSLIYYFFCGFRADGGQFLTFFGIILLHQYIAVCFAMTCIAVSRNFAGASLVANLAYTLQSMACGYFIQSNTIPVYVRWTKWTAYVFYAFGALCANEFTGAFYDCPLEGGASNPACKEYTGAYIMDSLGFPHDWVWRPILALLGFAIAFYIGAGIILKFWNAEIAMARARPSNMDASAGKEKMGERATGDVRTINIRLEGYGLDIEKRSLRKRWTKSILKPLTADFRSGSLNVIMGPSGSGKTSLLNNMAGRLKDDMSTRYKQYGTMTYNGLAPSQEVVHSICSFVTQDDDALLASLTVRETLRYAAGLRLPKWMSKEQKVQRAEEILMKMGLKDCADNLVGNDIIKGISGGEKRRVTIAVQILTEPRILLLDEPLSGLDAFTALSIMDVLRGLANEGRTLIVTIHQPRSDLFAHFGSILLLARGGYPVYAGPADDMLRHFAAQNYECPRHVNPADFALDLITVDLQHETREVASRAKVRKLIESWSSDMFPVALARTGSIATPAELGSLAREPASFASAYAILIRRATKNMFRQPEILIARIMQVVGLGLVLALYFAPLKTNYFAIQNRLGFLVEIAPLYFVGMLNNIAVYPIERDVFYRDYDDRIYGVEAFFLTYISITTPFEIISCLVFSILAVLACGLERNVQTFFIITFNAFCITSCGESLGIAFNTVFTHTGFSVNCMSVFLSVAQVMGGVLSLDVPDFLQAWNHLSPIKWAVGNMAPYTLRDLSFTCEDWQKINGQCPITTGEQVLDLYKLNKNPEMNLMALGICAIAYRFLAYVVLKVVKERWIGRLWRKVGGGKKKTNTQTQTSQTSNAAAVGNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEF7</accession>
    <name>A0A9W8YEF7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003653</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEF7"/>
    <dbReference type="OrthoDB" id="407658at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4373358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="158" mass="18206" checksum="62BD8E3256208591" modified="2023-11-08" version="1">MAKHVLQQPAYRDKALYQPAAWYDFHGPLTAKSGLFMQPQPPWPLHPGHHSSYPPLRSRVRNMQAALGVIEYHGTEEGLDASTDHRTGYPRQDEVQKFWKAVAEARRVLSVAKDRGHTVEQGEWRDLVRRVKDAVELRAWATEELMWKVDVLKKALES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEH1</accession>
    <name>A0A9W8YEH1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_002474</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEH1"/>
    <dbReference type="OrthoDB" id="3808060at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4375387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="14605" checksum="F8C491CD329CFEA2" modified="2023-11-08" version="1">MMLNKALYGLLHTFNITSVSTPIKHESGSGNLSGVMSHVQFSTREEATTVIDSLDGTVLFGRRLRVVESHIPMKYLGGRSWDSGLPGTHNSGRDQGSAVGTNFEAMQEELFRKAILRSNDRKLWVPSHKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEL8</accession>
    <name>A0A9W8YEL8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_001085</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEL8"/>
    <dbReference type="OrthoDB" id="1875751at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12577">
        <property type="entry name" value="RRM1_Hrp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000466">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein HRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000025">
        <property type="entry name" value="RNA-binding protein Musashi homolog 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034156">
        <property type="entry name" value="Hrp1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48031:SF2">
        <property type="entry name" value="RNA-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48031">
        <property type="entry name" value="SRA STEM-LOOP-INTERACTING RNA-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="83"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="150"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="294"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="340"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="382"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4375808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="460" mass="49076" checksum="D113DB612DC0B557" modified="2023-11-08" version="1">MFIGGLNWETTEQSLKDYFSQFGEVSECTVMRDSATGRSRGFGFLTFKDPKCVNIVMVKEHYLDGKIIDPKRAIPREEQERTSKIFVGGVSQEATEEDFTSFFKTFGRVVDATLMMDKETGRPRGFGFVTFDGDAAVDATLKGPLQILGKPIEVKRAQPRGNMKDDDGGNDRKFGRGNKFNNRDNGGGNGQGNGYDNQNNQQQGVGGNSGMSPAMMAQYWQRMQQYFQSMQQTLAAQQQGGGMANPMGSMGGMNPMQMQQMQQQMMNQGMNRGTGSPNPQMMGGMGGMNPMQMQQMQQMQQMQQGGGGGGGQGMMSPQGGNGGAGGPRPAYTAQEQMAFEQQKYEQQQARRQQQLGGYGGAGGPTSWEGMYDDTPQPSIPSGPGGARGGGFGKQGGSVGGGGHSRGSRQGSSQPPTGPSNAPINAPTGPKNAGKPGANYRGGGRNNNNYRHNPYGGGRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YES0</accession>
    <name>A0A9W8YES0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DASH complex subunit SPC34</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Outer kinetochore protein SPC34</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">N0V83_002462</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex SPC34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YES0"/>
    <dbReference type="OrthoDB" id="3693967at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013966">
        <property type="entry name" value="Spc34"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08657">
        <property type="entry name" value="DASH_Spc34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ4375376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="104" mass="12412" checksum="A20B9C0A1FE1F251" modified="2023-11-08" version="1">MNCPIPGAHDKIAAMRQRHVQVTASIDYYESRLAEQQIQLNRLNRSRDFVHEDVDDVEEEPQNQVPLTEEDLRREEDEMRLLERKKRELEDRVHSMSRDISGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEW1</accession>
    <name>A0A9W8YEW1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Threonine dehydratase</fullName>
            <ecNumber evidence="12">4.3.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Threonine deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ILV1</name>
        <name evidence="15" type="ORF">N0V83_003059</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>L-threonine = 2-oxobutanoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:22108"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.3.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from L-threonine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.19" evidence="12"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEW1"/>
    <dbReference type="OrthoDB" id="4418812at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04906">
        <property type="entry name" value="ACT_ThrD-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04907">
        <property type="entry name" value="ACT_ThrD-I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01562">
        <property type="entry name" value="Thr-dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1020.10:FF:000001">
        <property type="entry name" value="L-threonine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000008">
        <property type="entry name" value="L-threonine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1020.10">
        <property type="entry name" value="Biosynthetic Threonine Deaminase, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001721">
        <property type="entry name" value="TD_ACT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038110">
        <property type="entry name" value="TD_ACT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005787">
        <property type="entry name" value="Thr_deHydtase_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01124">
        <property type="entry name" value="ilvA_2Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006674">
        <property type="entry name" value="PRK09224.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF11">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00585">
        <property type="entry name" value="Thr_dehydrat_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51672">
        <property type="entry name" value="ACT_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7 12" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="10 12 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ACT-like" evidence="14">
        <location>
            <begin position="395"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="ACT-like" evidence="14">
        <location>
            <begin position="491"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="36"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4374318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="572" mass="62407" checksum="704A79437A201695" modified="2023-11-08" version="1">MAAMTDYSRPTTPEHGTNGIGSPRTPRQTGLALTEYTANPSPPSESAQQRPKQVVPEAFLLPNGYPDYLRLILTSRVYEVVHETPLTHATNLSNRLECNVLLKREDLQPVFSFKLRGAYNKMAHLDPKDRWRGVVACSAGNHAQGVAYSARTLKIPATIVMPSGTPDIKHKNVSRLGGSVILHGADFDAAKAEAARLEKLHGLVSIPPFDDPYVIAGQGTVGMELLRQTNLQNLKAVFCCVGGGGLIAGVGVYIKRIAPHVKIIGVEQYDANAMVESLKAGKRVFLNDVGLFADGAAVKTVGEETFRICQEVVDEVVQVTTDETCAAIKDMFEDTRSIVEPAGALALAGLKKWVSRNPSPDSDRELVAIASGANMNFDRLRFVAERAAIGENKEAILSVTIPERPGAFAKLVSVIHPMAVTEFSYRYSGPDEATILVGVELKAATRSRDLPDLIARLGAEGMTAHDLSQDELVKSHIRYLVGGRSSAADEHLYMFEFPERGGALYKFLHTLQPGMNISLFHYRNYGGDVAKILAGIQCKHEESGKLASFLKDIEYPYKEVTDSPSYKTFLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF49</accession>
    <name>A0A9W8YF49_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_001138</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-beta-lactamase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF49"/>
    <dbReference type="OrthoDB" id="10250730at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051013">
        <property type="entry name" value="MBL_superfamily_lactonases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978:SF5">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978">
        <property type="entry name" value="QUORUM-QUENCHING LACTONASE YTNP-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="5">
        <location>
            <begin position="36"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4375860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="285" mass="31934" checksum="52D513B7B0E88A4D" modified="2023-11-08" version="1">MGSRKDWWNLPPVVVEAIEAKGVPGIRIDKDISEILSTGEVDPKTIDAVVWSHYHWDHVGNIQRFPLSTDIVVGSGFKKSFTPGYPTNSASPFYDADFEGRTIQEISFAGDQILKIGQLQAFDYFGDQSCGDISHFPGTYRPTNHVPMPETIPSETKLDHRIPQPCPCTLFTACHPRGPLKARSTPYYDPSTSEESWYDDAAEAKISIEGMAEFDADENVFVAIAHDPALQEVCEQFPHATMNQWKSKQWKLQSHWNFLNELPLGGQPGRPKLVDGRFRDGVRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF71</accession>
    <name>A0A9W8YF71_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Carboxylesterase type B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_001599</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF71"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11058">
        <property type="entry name" value="CYP60B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF230">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 10" id="KW-0408">Iron</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040759920" description="Carboxylesterase type B domain-containing protein" evidence="11">
        <location>
            <begin position="21"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="12">
        <location>
            <begin position="22"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="969"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4376316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1027" mass="115539" checksum="9294123A78B8C363" modified="2023-11-08" version="1">MVNIQTYLLLGAPLIANAVANPPVVRSNAGIFHGRYLPEFDQDLYLGVKFAPKPVRFAPAELVKDTPGQQFNVTQYGIDCQGYGSDTNLLVAGNWTRLGEDCLHLNIIKPKTKKTSLPVLLWIYGGGWQQGSTSDPRYNMSYVVQQSVLNNKPVIGVSINYRMAAFGFINSKEIAATGNQNLGLRDQRLAMKWVNKHISSFGGDPAKVTIWGESAGAYSVGDHINAFDGNSEGLFRAAILESGGAVGPPLNGTDWYQHMYDNLTSSVGCSNATDTLQCLRSVPFETIAPYGYLGLEWFHTLDGSFITRYGQQSLTSGAFSKVPLILGTNTDEGFGVNGVNTDDDAINQLVHSKRWVINEEQARKLLELYPNDPSLGEPYGWGNRTWPEYGAQYKRYQSIATDLTMFAPRRLLAQSMSKYVGKVYSYRWDAPKFNNTPSTIGINHFSEIPFTFGNTQQQITPLGNSTENRALSKLVMRMWTAFAYDLDPNGHGVENITAWPKYPSNASNFVFRTDKSYVEKDTDRAEGVAYINTIVDRSLTILRAPKPDKHRTMGLQFLGNTGGVGIIGTAVGVAFVYLVTNLIYNVYFHPLSRYPGPFWWRATRIPFCRELIGGTLSFRILDLHKKYGQIVRVAPNELSFSDATAWKDIHGHRAHLDKWDGFYRPIKEMPTDIVSSSREEHSSLRRLFSHGFSDRALREQEPLLSKYVDLFMNRMHESSGKEKGPVDICSWFNYLTFDIIGDLVFGEPFGCLDQSNYHPWVKSIFEMGHVGVYIQTASHYPLVKKILLSLVPESAKKKRDSHLEFTKDKMRRRMAFKEDRHDLIEGLLKTDLGLDELEANASIMIMAGSETTATLLSGLIFYLTTSPKTLERLTAEIRLAFDREEDINLQSVNKLPYLSACLNEALRLYPPVPNGLPRIVPAGGATIAGNHIPENKPFEFHPERWLNDDAFQNDDLDALQPFHIGPRNCIGRNLAYSEMRLIMSRLLLNFDVKIAESSRSWIDQKIFLFWEKGPLNVYLTQREGSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGN3</accession>
    <name>A0A9W8YGN3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_000451</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGN3"/>
    <dbReference type="OrthoDB" id="3231000at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="679"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="754"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="786"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="21"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="202"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="831"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="207"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="299"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="330"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="355"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="434"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="832"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="852"/>
            <end position="863"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4377624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="874" mass="97747" checksum="3BC445730333044A" modified="2023-11-08" version="1">MRYTHGIRALELTDEHYDNELSDHEHMVDKGAQKPKNVIEDRREVENTYDPKSDWETKDITSGGSVSKTEDTAVPAQGQDQQQLNRQQSDDALEEDERSKKQGRRVYEAEIEHGRAQDVRGSNIDTEVNQLPSSGHDGYRTSLHDLQVVPLSLVWPTASFARGSQISLPLSGTSSLATLDPSQRERLPLGASLERLMLRPETIALPDRTDNLSEHTDLDDWDRDSSSKPQDQQDRKARRPEGARNDLENPILGWTMLSGIEPKKGKARKKGGAQRAPPSPPVAPEVEAYRANVNGRNLRNKDTPQIRSVDEWVAQTSDPSAVPTSRAPASSLLPSVPESGPVPPPPLPNASSSALPPPSAPPPPPLALPSERDQSRATGAKPQTSEADLTPAALRRRATKSDMEIPSDQEYGGKPNISNKTPPDRNRVHASPLGPMPNVPPPPPPPVDDSLITETHGPFVPYHPIKARQSSSAKIGNTFTARHIRAYIHSLQVPTSTLQEFEYHLLLPNKGKETEHDPFRALFRVIHDDTHSLVDIIRISQQRIREGTMDEDLMQKRVTFWRSLLHRLNFNLAEVEQRLRAFVHFAYDSETHPFTFDQRTELLSEKLAKETRQTLRSCIDLLDKSSNSLLAEMQIVDSRRSIAEAESVSKLTELAFVFIPLSFVASLFSMQIHELDEGVSLYLFVVVAIAFVLVAYVVRLSIRSSRLLEYKSKMLLQIRDDSHIQYNQSIPTHTFLRWVGKAMGGTASKYFKDVFAVCAPLILLLAIFAAILSPIVLLWLRNISKGFTAMMTVLLLLLDMVLVYPVIVSASGTFQLNPMAIVRQIQRDHEISRKRKQKAKKRRKEKAGIDPESLDVESSDESDKDSKRASVSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGV2</accession>
    <name>A0A9W8YGV2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_000229</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGV2"/>
    <dbReference type="OrthoDB" id="429813at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051414">
        <property type="entry name" value="Adenylate-forming_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43439:SF2">
        <property type="entry name" value="ENZYME, PUTATIVE (JCVI)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43439">
        <property type="entry name" value="PHENYLACETATE-COENZYME A LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23562">
        <property type="entry name" value="AMP-binding_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="25"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4377404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="539" mass="61510" checksum="60EAA3B6AF2FB69B" modified="2023-11-08" version="1">MGSMPESDMRDPPGRLLVHAIEEKAQCVPYHTYIRYAPADWENNGYRSMTWRQYANAIDKVAYWLDQKLGKATESDTVAYHGPNDPRYAILVPACIKAGRKLLVPDGRVTKEGLMGLFATTNCKTWIYAEDDRSVPLIEADSGLQICALPSFEWILQDEKQKRYPYDKTFEQAAWDEIVIIHTSGTTVLSKRHWPRGITYESWIGKNCLNPCPPQWLAGLHTMIISPVFLDCPCILPPTDVIGLPLDIFKKIYSMNQVHGIKCPPHTIVTLYEDPEARELLKSMEFLLYLGAALDRAIGDDLCQYTRLTSIIGSTETGEQVSLRPANRMLWYTHDFVPENGHKMIPIDEGGASEEDLHELVFDSSEHGRTNPFQLAFWNPAHKDMKRIETKELYKPVIDSDGRTRWVFAARKDDLTKLSWLAKFHAQDIESRIQQHADVRGVFVGGEGRPTPYVIVETKEGVLDRKSEEQLLEELYASAIAGTSETDIDEIRIPKETVIIAKKEKPFKRNLKSVVRRKEVEQDYLEEVEQAYLQLANVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHE2</accession>
    <name>A0A9W8YHE2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Proteasome regulatory particle subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RPN7</name>
        <name evidence="5" type="ORF">N0V83_000378</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHE2"/>
    <dbReference type="OrthoDB" id="1452at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005838">
        <property type="term" value="C:proteasome regulatory particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000013">
        <property type="entry name" value="Proteasome regulatory particle subunit (RpnG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000021">
        <property type="entry name" value="Putative proteasome regulatory particle subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019585">
        <property type="entry name" value="Rpn7/CSN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045135">
        <property type="entry name" value="Rpn7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049549">
        <property type="entry name" value="RPN7_PSMD6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145">
        <property type="entry name" value="26S PROTESOME SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10602">
        <property type="entry name" value="RPN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21154">
        <property type="entry name" value="RPN7_PSMD6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="4">
        <location>
            <begin position="228"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="299"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="105"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="314"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4377553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="485" mass="53422" checksum="661CEAB1B1CD340B" modified="2023-11-08" version="1">MGDPQYAKLPDLQLAQHIFQLTNPAASKSAKQSSLKTVQDAITEHKMAPLYRYLAHPTDGVLNAPGEGSADQPTSHRRPSASIANMLATRNPSLEVPLSWDEKLYESLKAENEKELDAIQKEEDEASEKAGETEVQAARGKRAELYNRIGDKDKAIASYEAIFEKTGILGTKIDLVLAIIRVALFFGDRILAKKTIDRASALVESGGDWDRRNRLKAYTGLHYLTVRSHSQAAPLLLDSLSTFTSYELCSYSNLVVYAILAGSVSLKRVDFKSKVVDAPEIKAIVGTSEEKLAAITGQTSAGPAAGDEEMGDASGSTATPVPTAVNLTTLGGPQDKEVPVDFSPLAKLVKSLYEGDYKSFFGALSDVEVNFLTQDRYLYEHRGWYVREMRLRGYQQLLQSYRVVGLESMAKDFGVTVDFLDKDLAKFIAADRIPCTIDRVKGIIETNRPDDKNKQYADVVKQGDQLITKLQKYGQSVRLRGSERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHM8</accession>
    <name>A0A9W8YHM8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Hexose transporter hxt5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">HXT5_1</name>
        <name evidence="11" type="ORF">N0V83_001316</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHM8"/>
    <dbReference type="OrthoDB" id="2241241at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000044">
        <property type="entry name" value="Hexose transporter Hxt3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF91">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="41"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="343"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="369"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="403"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="450"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="473"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="44"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="549"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="558"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4376036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="579" mass="64761" checksum="92AD17ECA4FF53B4" modified="2023-11-08" version="1">MKLTNPLRQWRNKDPDEQRSQWVGEHAVYDNSPLPRLTWKGFWMGILVSMGGFIFGYDTGQISGFLEMKDFLNRFGQTNSDGTRYFSNVRSGLIVALLSIGTLFGALAAAPIADKIGRKKSIMAWCGIFSVGIIVQIAATDKWYQIMMGRFVAGLGVGALSLLVPMYQAETAPRHIRGALISTYQLMITFGIFFAAVFNYAAERHQSGKAASWQITLGLSFVPAIILAVGILGFSETPRFNYRHGKIDKATNTMSRVYGVLPHHHDIQLELEEMRVQLQAETKKGNAIQEWIGMWRAPKMAYRLAIGMGLQMFQQLTGANYFFYYGTTVFAGTGINNSYVTQMILNGINFAVTFYGLYIVEHYGRRKSLIAGSVWMFICFLIFASVGHFALDRETPQNTEKAATAMICFACFFIFGFATTWGPMIWTICGELYPSRYRAKAMALSTASNWLWNFLLAFFTPFITGAIDFRYGYVFAGTNILGGLIVYFFVIEGQGRTLEEIDTMYLMGVKPWESSKWVVPNLEEMSGKMRERLEAGNPELAIKKEMAGAHEGGVGGARAEKNGRHGSEEGLKEKSEVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YI72</accession>
    <name>A0A9W8YI72_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoacetylglucosamine Mutase</fullName>
            <ecNumber evidence="3">5.4.2.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PCM1_1</name>
        <name evidence="3" type="ORF">N0V83_000999</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.3" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YI72"/>
    <dbReference type="OrthoDB" id="1928at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004610">
        <property type="term" value="F:phosphoacetylglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45955">
        <property type="entry name" value="PHOSPHOACETYLGLUCOSAMINE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45955:SF1">
        <property type="entry name" value="PHOSPHOACETYLGLUCOSAMINE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="2">
        <location>
            <begin position="15"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4378166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="175" mass="19109" checksum="925A8478FF159595" modified="2023-11-08" version="1">MLQEEWEVLSTEIANQATPEDVSKYYHEVASKYKIDLNTPARVVVARDTRASGSRLLGCLLDGLKAAGAEPKDYGFLTTPQLHYMVRCLNTEGTKEAYGVPTETGYYEKFGAAFKTALKGKKPSGHLTVDCANGVGGPKLAELIKYLPPKEEGLEIFVVNDNVIKPEALNVDVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIR5</accession>
    <name>A0A9W8YIR5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000933</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIR5"/>
    <dbReference type="OrthoDB" id="3693435at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4378101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="277" mass="31322" checksum="ADF8EDFDFD8317C6" modified="2023-11-08" version="1">MDMLPSAEELKGSKIDIEVSPGVVKRIPAAEGLKREVERYLPPSGRYYDQNTVEAIFASSIFAGRGRCVSCWSPKHVLSMRRCKRQCCVCGTEEHLGLECPALYATWRWWREHGHTPSPAIQSRPTTAQLAYLIVAKVVKPIENIQGPLIVNMDHPAVREFYQGKAAPEVILSQPKEPEVDTDARVHPNTSNHDHPDLAHRHCLDHIRQLENKIGAMENRIQSMESTLNIVLDAIRDTILDQTHKNEERLTALEYTLGMGEVKTSEERRSLEDDADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJ16</accession>
    <name>A0A9W8YJ16_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1996 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001787</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJ16"/>
    <dbReference type="OrthoDB" id="74764at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018535">
        <property type="entry name" value="DUF1996"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43662">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43662:SF2">
        <property type="entry name" value="DUF1996 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09362">
        <property type="entry name" value="DUF1996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1996" evidence="1">
        <location>
            <begin position="2"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="139" mass="14712" checksum="93BFF1B7B1DDAAE1" modified="2023-11-08" version="1">MAGFRMLAGDPTLRSKTGTSPGICHRCLKANEAITGGSGAPCSSSDTAGFPTKPCPGGIRATIIFPSCWDGVNLDSPDHKSHVAYAPGNSALAGDKCPSTHPVRIPQVMYEIMFDTSKFTNPDYFKDGKQPLVYSFGDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJ41</accession>
    <name>A0A9W8YJ41_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000045</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJ41"/>
    <dbReference type="OrthoDB" id="3799196at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="69" mass="8053" checksum="F5EA57D60AEBBF45" modified="2023-11-08" version="1">MPAREMRETKDGVRWKEVPYNEKREVMKRINVQLDAEGIPEVTLQVVSWRMSKALVNLKQSNPKQSEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CGD9</accession>
    <name>A0A9W9CGD9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peroxin/Ferlin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_010949</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CGD9"/>
    <dbReference type="OrthoDB" id="72441at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006614">
        <property type="entry name" value="Peroxin/Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051513">
        <property type="entry name" value="Tectonin_beta-prop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23250">
        <property type="entry name" value="DYSFERLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23250:SF1">
        <property type="entry name" value="TECTONIN BETA-PROPELLER REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00694">
        <property type="entry name" value="DysFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peroxin/Ferlin" evidence="3">
        <location>
            <begin position="107"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="313"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="250"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="332"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4362008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="341" mass="39262" checksum="116E00D8C0F41B67" modified="2023-11-08" version="1">MADQQINLVDHTDGEATPTTQSEHPPSTPDSHIDILFENQRGWFVFGIPLFSSKALWNFKIDPAPWVDAKFKPSAVNITNAQVPDPSWVWDWKSWYVDMSLDVDEEGWQYSLLFKGSPWHGNHPWFHSFVRRRRWLRRRVKQRIPPKTKESQRERLFGETFSIGTTLARSMTVGTGTLSTQDSTQATLDEEVRDIATLMRKLKKAAIDREKIIIIHKFIDDGGEELHFLAEQIPTIMSMLIFQNSRRQLLSTLMDRFETAKAHREEHKKEDKPEGEAEERRINNLLKAIEAADSECKKLEYWSDIKNLAEEGKAGEAMDPSRGWDEKWQGLDGSGAKGPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CGZ4</accession>
    <name>A0A9W9CGZ4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cellulose-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010692</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CGZ4"/>
    <dbReference type="OrthoDB" id="3592035at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="entry name" value="Ribonucleoside hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048527">
        <property type="entry name" value="Sde182_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011483">
        <property type="entry name" value="Sde182_NH-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21027">
        <property type="entry name" value="Sde0182_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07632">
        <property type="entry name" value="Sde182_NH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cellulose-binding Sde182 nucleoside hydrolase-like" evidence="1">
        <location>
            <begin position="13"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Cellulose-binding Sde182 C-terminal" evidence="2">
        <location>
            <begin position="367"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4361752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="477" mass="53502" checksum="A2B800D49411D631" modified="2023-11-08" version="1">MAHALNSYLTKPRIFILTDITNEPDDAQSFCRYLTYSNQFDTEGVVPTTSTWLRDKVAPEALHDIVDGYAEVVDNLNAHTHPSNPYPTAAHIRTLIKPGAPVYGMEAVGDDVPLSEGAELLLERLQSDDERPLWVLVWGGTNVLASVLYRIRNESNAAQLRAKLRIYTISDQDDTGAWIRQQWPELFYICSVHGWNNYWSAAWIGISGDMPADRGAADRSMVTKEWIKENLQIGPLGKKYPDVMYAMEGDTPTFLYMIQNGLGVPEEPSWGSWGGRYLSVNVSPKGLPSRGHFADVDDEVVGIDGNKHRSTRATVYRWRNAFQSDFAARMQWTLTSDFSKVNHQPVVDVDGNQGFAPILVDTDAGSTVAFDASKSYDPDGDQLTFKWWQYREPSAVQTYHSAEVVSVELKPSEDGKRVEVTIPPPEKSCAIQRSNPFRMSRRGSPLHLILEVTDNGTPALTTYRRIIITPVNKSFQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHJ1</accession>
    <name>A0A9W9CHJ1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4211 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010568</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHJ1"/>
    <dbReference type="OrthoDB" id="21499at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025451">
        <property type="entry name" value="DUF4211"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14689:SF0">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14689">
        <property type="entry name" value="PHORBOL-ESTER_DAG-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13926">
        <property type="entry name" value="DUF4211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4211" evidence="2">
        <location>
            <begin position="370"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="507"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="515"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="659" mass="74300" checksum="F298CF08ADC18015" modified="2023-11-08" version="1">MAPKRARERKRQIKLDFTSAAKSSSSTPSASTTMGNSASGPVANTPKTASNMLPRKASSKALIYSSSEDELQAETDRGMRLGLPVESTPHGMFDGSDVDAASSESEEDEEEVQVKAKPTTRKRRRARDNTGSESEEAFTVLPRKRQRHSPAEVEVSDAEEVEEELRLPSKRRKKTRQDTPDEESEEKTPPRSTKRKVARRRPTSDEEQEEQAPPRSSRRRIVRKRAPSDDEDEEDLDNANESGHGKSDGEEDEDLQEDLAFLRSSPLPDRGRLRSTQDKPKNERQKALEALKKRRAGTNEPSSSSTPSRKKPVVVDTESESELEVIKEEQDSDLEELDPVEDDVDDEDEGDDREANILDMFQEDGDDAGFIDDDADAIIGEPSATADLEELRIVAGLSRAKSKDLFKHAIEWMVMKKIHPGFNSTKNIYTITFRKLDDEVKGLAGSKFTSSVWTPDFTRAIRARPDLMLDEISFGRREVMSPHCEACNRSNHTATFELMLTGEPYDPETLEPLGNDSDSSSNSDSDADSEISADSEVLLNGEKPAYNAQGEQLPPESHRFALGSTCKANAQVAHTLHHWRYHLYQWVKDYLAREGHLTAEKLVKRDKWNDKKRQKAAQKIVDGMERDGEVKKLYKLYEEQVEFAMEAVNDYRNGWGRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CID0</accession>
    <name>A0A9W9CID0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_009506</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CID0"/>
    <dbReference type="OrthoDB" id="73875at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF397">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="6 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH18" evidence="10">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4364051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="220" mass="25180" checksum="47500C74E77D32B3" modified="2023-11-08" version="1">MTPDEIPVHDISHLIFAFGFITPDEFKVTNMPDVRPDLFQQVTYLKNKNSDLVIQIALGGWSHNDPGKWQPVFSDMVSTQANRAKFIKNLLGFLSQYGFDGVDFDWEYPGAEERGGKKEDGVNYTLLLQELRQAIQSSGKNYIVTFTAPTSYWYLRHFDVTNMVQYVDWINVMSYDLHGTWDAENPIGNQVLGHTNLTEVDLALDLVSDSFGFTSHREWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIN6</accession>
    <name>A0A9W9CIN6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 5B</fullName>
            <ecNumber evidence="3">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Translation initiation factor IF-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">FUN12</name>
        <name evidence="16" type="ORF">N0V83_008990</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIN6"/>
    <dbReference type="OrthoDB" id="4928at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03703">
        <property type="entry name" value="aeIF5B_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000013">
        <property type="entry name" value="eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000026">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000112">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029459">
        <property type="entry name" value="EFTU-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003078">
        <property type="entry name" value="PRK04004.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14578">
        <property type="entry name" value="GTP_EFTU_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 16" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="15">
        <location>
            <begin position="484"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="68"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="210"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="303"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="355"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="371"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="399"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="425"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="457"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4364396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1078" mass="119123" checksum="E059B4A661FCEF13" modified="2023-11-08" version="1">MPPKKKGNKKAQNDDWEADLGESIVPQDSAAAEGNAEDAAPEDDTAGGGLLAALQKRGKKKNKKNADFELEGEDPPVDGADGDLDNKAPQEATFDDDDDDVFAGNYGKKKGKGGAAEKKDEPEENAENADDGPRVKTKAEKEKEKKEKEKQRKKELAAKKKATAPAKAEPAKAAESKQEPAAAPATDTSGPAAGGKKKKLNPALAALQKQQEERKKREEELARLEAEEKARLEELEKQDAEEQKRKEEAKALKKQKEKEKIEQLKKEGKYLTKAQKEAKAKNELRLKQMLESGGAKVAALDEPEQKKKPMYDDRKKRKKQIESQKEKEAREAEEAAKKLEELRIAEEQAAAAAAEAEKARKDAEAKKGDDSATEDWEAEADELEGVKDSWDVDTDEEEERKATEKKAKEAKAAAEKAAAEKEAQAEDEDSDSESDSEDDSEDDSSEDEQGTATQRAEAARKAAAAERRKQAHEEALAARSKDNLRSPICCILGHVDTGKTKLLDKIRQTNVQEGEAGGITQQIGATYFPVAALEKKVAVVNKDNDFVFNVPGLLIIDTPGHESFTNLRSRGSSLCNIAILVIDIMHGLEPQTIESMKLLRDRKTPFIVALNKIDRLFGWKKIDNNGFEDSFGLQKQAVQAEFEERWTFVRTQLQEQGFNSELFFKNKNMSKYVSVCPTSAHTGEGIPDMIKLIVKLTQERLTNNLMYLSEVECTVLEVKVIEGLGTTIDVVLSNGVLHEGDRIVLCGNPEPIVTNIRALLTPAEMKELRVKSQYVHNKEVKAAMGIKISADGLDTAIAGSRLLVVGPNDDEDDLMDEVMGDLAHLLSKVSKTGRGVSVQASTLGSLEALLEFLRVSKIPVSTISIGPVFRKDVLRAGVMLEKAKEYAVMLCFDVKVDKDAKAYAEEIGVKIFEADIIYHLFDKFTAHMKQLEEQRKEESKMLAVFPCVLRPVAVFNKKDPIVIGVDVTEGNLKMTTPICAVKKNPVTGAKEIIQLGRVTSIERDHKQIQVCKRGQPSVAVKIEGANQPMYGRHLEEGDTLYSAISRKSIDTLKEFFRNDVSQDEWKLIVQLKGLMDIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJU9</accession>
    <name>A0A9W9CJU9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007801</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJU9"/>
    <dbReference type="OrthoDB" id="630895at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051531">
        <property type="entry name" value="N-acetyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43792">
        <property type="entry name" value="GNAT FAMILY, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G00765)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43792:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="29"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="219" mass="24742" checksum="C593B3ADCA3FE976" modified="2023-11-08" version="1">MNPIIETPRLLLIRLTDTTEGSQHVQWFHENWSDGEVTGWSLAESREWMLEHRCKYDNWFYACFVKTPDSKVTELDPGEHAGSVSLRLQASGPALPPPPSFDPDSGREGLHPSTASPANIPIDLRAIGYAFFAQHRGKGYATEAGKAMLAEYARSVAEEKAKGERLFYVEAGVDKDNPESQHVLKKLGFKEVGWKEEKEPVFLNGQWRDPGYWVYGQYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJZ1</accession>
    <name>A0A9W9CJZ1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_007402</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFB3 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJZ1"/>
    <dbReference type="OrthoDB" id="521512at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012576">
        <property type="entry name" value="NDUFB3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15082:SF2">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15082">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B12 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08122">
        <property type="entry name" value="NDUF_B12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4366874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="9137" checksum="4539D4CEAE63ED10" modified="2023-11-08" version="1">MAPRPSITGFDPKKFAAASANGTKGDPWARYEQWRYTGPFTRFNRFKGAFPGLGIATVAFAGYLVAEQMFFKDEHHSEHHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKS4</accession>
    <name>A0A9W9CKS4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_007158</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKS4"/>
    <dbReference type="OrthoDB" id="3673852at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="53"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="120"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="153"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="186"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="219"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="409"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="527"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="597"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4367574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="669" mass="73271" checksum="6C0D49BD001599F5" modified="2023-11-08" version="1">MELDGAEEPFLEESRRATTEDFAAFVAAIRGGDIEVVESKLTLGVDIERETDDGNIALVISIKEDKVDIARLLLEEGADVHHRVRRLPPIVHAVMTNCAPQLIQLLVNHGANLNAVSGPDQMNALHWAAVEGKVDAVDFLASKGMDLNSASSKGRTSLILAAEKGHTTVAKLLLAKGAELLKRSDNGGTALAWAACNGYLDTVKYLLEEGIDVDDCDDCGLTALSVASNSGHVEVVEFLIEEGADINSLSVEPKHFTPAMAAAIKGRTDVIQAFIKHIPDFGIRNGNGETVLDIAMAEGHFETVHVLLGYLGGPNHPRDSVALHFAMANSYHAVKSLVATTSMTYLHLHPNPTQFGNFDWMSCVLQMTGFLVWPKAMTKMMHYALIEEQHDMIKALLAHGCDVDTYLPTGHTPILLATERQKPEIIRILLDAGADPAKPGRGLEHAHVTPLYKALVALEQDREKDTSLVDQLLSSGRCKLLQGKSFQRTAFSYIIRRFDEWDHGVAEILAFRMLEAVPDVTKERADDGSTLMHVAVQYNRQDLIDILLQKGVDINTPDHHGQTPFLMKCWRHQPRNLPSLLPFLLERGADAFAKDKEGRSALHNAAYQGSIATIGLLLSKGLDIDAVTIDGETPLICALITAQTPPRGVIPWQDDQCEDLLYHGQKEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKT4</accession>
    <name>A0A9W9CKT4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_007068</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKT4"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4367485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="195" mass="22086" checksum="1FE338451B1C87B5" modified="2023-11-08" version="1">MAIQALSALFRLRDLSAIQVPTATAFDLDEGSDFKLEEIERLVRLAAKSITDCPEGKLPKLEDETPQEHSHRAQSVFAEKKAAVSEKLVAALKRKWSINHLALPRAKEFSSYFHMDTVGTQIIDQLNAWRDNKKLVEYLERLSRVLVHQEVIAISTPHYSFAPPPKHDKELDAARYYGSVDIFNAPAPILSHDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKV2</accession>
    <name>A0A9W9CKV2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_007249</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKV2"/>
    <dbReference type="OrthoDB" id="6365676at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF120">
        <property type="entry name" value="KRUPPEL-LIKE FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="163"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="196"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="135"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="209"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="144"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4367664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="329" mass="35321" checksum="7C6A98016C307E42" modified="2023-11-08" version="1">MDALSGKNSTGRRVSLLNDGPEPPHQLVRLPSIPPSLRSRTSSYTSSPIGSPPTPRLVRSDSSDSANMHTPSPITPDFVFEGLPVQGMDSPVFSQSGFFPPQKDLNSAYPPVPQSTGALPYHTAPASQAGYFRPEQMQDSRDSAAAAAAAAAANNARPKKNSYPCPMAKQFNCNDYFTTSGHAARHAKKHTGKKDAFCPECNKAFTRKDNMEQHRRTHQSGRNAAKGGDRDIKKVKQQTKRPRPAPLQSSMPSLSGLSMVDPSLPVSPAGSFLAPAVQNTDPFLDFAQRSPYPDPTAYSMTHSYTAGSSYGGLDALAIAASGEKRKFES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL36</accession>
    <name>A0A9W9CL36_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pre-mRNA-splicing factor cwf19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cwf19</name>
        <name evidence="5" type="ORF">N0V83_007251</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWF19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL36"/>
    <dbReference type="OrthoDB" id="2113965at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040194">
        <property type="entry name" value="Cwf19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006768">
        <property type="entry name" value="Cwf19-like_C_dom-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006767">
        <property type="entry name" value="Cwf19-like_C_dom-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12072">
        <property type="entry name" value="CWF19, CELL CYCLE CONTROL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12072:SF5">
        <property type="entry name" value="CWF19-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04677">
        <property type="entry name" value="CwfJ_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04676">
        <property type="entry name" value="CwfJ_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cwf19-like C-terminal" evidence="4">
        <location>
            <begin position="474"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Cwf19-like protein C-terminal" evidence="3">
        <location>
            <begin position="582"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4367666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="718" mass="82891" checksum="F1F95F86AF9D1C79" modified="2023-11-08" version="1">MSGSENCANLEGIAEKTRERNAKKKRIQQKERDEQEDREDEEEERLRNEENDRAQSESKPRQASPQETPRETPEERARRKRKERRSRSPESDDDERLTRKRKSRHYDEDESSGDDVPPAKDYAPPDDDILDHQLEEAADANLQRDDWMKAPSSMDVDYVQRKKREEKTTYVRESAAQQHALKVHQAELNHHLADLKDEQGMKAEEPAQREVNYTFGDPGAQWRMTKLKGIYRNAEESGRSVEEVAMEKYGDLRDFDEAREEEIELDRRNMYGKDYVGKDKPSGELYEERRLKAGIHRPSREHHEAEELPQGEVVPDPRPTANTVMLSQTELNKLKAQMMKAKMKKAPNAAELEAQYNAALANSAGGKDRDVVVLNAMDNRMLAGGRQGEVTAITNKRGTERGLVKENEDMSIDDMVRQERRTKGQAGGEGMLLAEKIAKDSKFDNDLDYLDENATKFLPAMPPRRQITTPTPVAPIISLGTRTFLTLPTEPEISDGGAVIVPIQHRTNLLECDDDEWEEMRNFMKCLTRMYHDQGRDVIFYENAANPGRKLHAAMSAVPIPFELGDTAPAFFREAILTSDDEWSQHKPIIDTLKASREGQGKQAFRRSLAKEMPYFHVWFELDGGMAHIVEDERRWPKGDLFAREVLGGMLDTDFEVVKRQGRWVKGDDRRAEKWRKGWRKFDWTRVLTEEYEYLKMDSPCRHILESLPEGNERDPHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLG3</accession>
    <name>A0A9W9CLG3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[histone H3]-trimethyl-L-lysine(9) demethylase</fullName>
            <ecNumber evidence="2">1.14.11.66</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_005628</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(9)-[histone H3] + 2 2-oxoglutarate + 2 O2 = N(6)-methyl-L-lysyl(9)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:60200"/>
            <dbReference type="Rhea" id="RHEA-COMP:15538"/>
            <dbReference type="Rhea" id="RHEA-COMP:15542"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.66"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the JHDM3 histone demethylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.66" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="9547406at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:histone H3K36 demethylase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:regulation of gene expression"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Putative jumonji family transcription factor"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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        <property type="entry name" value="DUF7072"/>
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        <property type="entry name" value="JmjC_dom"/>
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        <property type="entry name" value="Znf_PHD"/>
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        <property type="entry name" value="[HISTONE H3]-TRIMETHYL-L-LYSINE(9) DEMETHYLASE"/>
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        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
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        <property type="entry name" value="zf-HC5HC2H_2"/>
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        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="JmjN"/>
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        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="8">
        <location>
            <begin position="68"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="9">
        <location>
            <begin position="344"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="10">
        <location>
            <begin position="601"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="155"/>
            <end position="281"/>
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            <begin position="974"/>
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            <begin position="34"/>
            <end position="43"/>
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            <begin position="47"/>
            <end position="58"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="7">
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            <begin position="187"/>
            <end position="202"/>
        </location>
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            <begin position="203"/>
            <end position="213"/>
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            <begin position="531"/>
            <end position="544"/>
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            <begin position="1102"/>
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        <location>
            <begin position="1283"/>
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        </location>
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        </source>
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        <source>
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        <source>
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            <dbReference type="ARBA" id="ARBA00049349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
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            <dbReference type="PROSITE" id="PS51184"/>
        </source>
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        </source>
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        <source>
            <dbReference type="EMBL" id="KAJ4369864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1365" mass="152978" checksum="235D843D06DE041C" modified="2023-11-08" version="1">MEVPTDVVPSVEAPHAQGADTATKAALTPPTSEDNDKRDERMSSELSDIDSDDGEDIEPDHYFEGGKIPVFRPTMDQFRNFKRFIDKIDKHGMKSGIVKVIPPKEWRNSLPDLSEAVRSIKVKNPIMQEFAGQHGIYTQANIEKQRSYNLPEWRAVTDEPHHQPPAKRGERRKAAAEAPSRSRSTRAQAAPANADDSSAPRRGPGRPPRRRQVKKEQIDEDDDAESIDVPPTPTSPGKEEKKEKKGPTRRIKKEAKDDATPAKARGRQPKTVDQKKSVSSRRLNNRGAVADYIDEAAFDDFDYHLPGLEEYTVERCKELEDNYWKTINYGQPMYGADMPGSLFDDRTTSWNVAKLPNLLDVLGTKVPGVNTAYLYLGMWKATFAWHLEDVDLYSINYIHFGAPKQWYSISQEDARRFEAAMKSIWPRDAKNCSQFLRHKTYLISPQRLEKDFNIKVNRLVHYEGEFVITYPYGYHSGYNIGYNCAESVNFANESWLSYGRIAKKCDCESDSVWVDVNEIERKLRGEPTPEYYEETDEEEDEDDGADHLPSPPPSVAGKTKAKSGRKPANTKRKRGKEDPKEAPRPKKLKRIRIRIRVPGRGMPCVLCPNDIEYDDLLPTDNGMKAHRICADYTPETFISVKAEGETVCNVANIGRDRLELKCNYCRSKRGAVFQCSQKKCTRAFHATCAVAAGVQVDLGPMPTFDEEGTEYFYDGYDFRCRFHRPKKRNNKTVDVESLEKDKFILAYGKTLKPKDVVQFQYVGGEMYQIHGAQVVENRPGEQAVLVDVLPDGDRVEVEWKYILKLHPDESQRPKPSVNAKPLPEHLKESDASLDINNRTDGVPEIGDPFHDPNSEQKWAEWHTAPEVTRGPAKVDFSKEDRLWYYLGKFSTEARSQYTENPAKPVNNPKSNFLDTVKPAPPPVPTFSRSSYPVSYPLKPAPIAVPPHTQMQHQIQPNRPYQYKPKEAMMTTWKSPVYNPDTRKNPNSPVAHQPNVSYDHRAPPAPYGQAPYQGYHSHRPPQSSQQQQFQYQPYVPPQSYSTTSWKAQPATGGSGLPNNQYAHTPQSGNVLSPYPYAQSPRPMPSSPFTQGAGPGMAPAFSASGPQSQAPSHGRASISNMLSNPPGTPKPPMYAISPSSSIVYAAQSSTEYLIYVLKYPYLKNAYLRRAKTYVSPYSMDGRIAPEWMPKPPASRSSGPPTAIASKPLTPNGSAPPQGYYQGIPQTGMPAPRPSVQFQSPDAFQREMARAPQPAETPKWEQMLKQLATSTGPTSVPPITASSPQAPQPRYPPAGPRSSLPYELPKSMQIVQSQSPPLPQAQEPQRPTPSPISDDGKGAGTEVTQKSVLPPLQSAPHQVHGGETWRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLG8</accession>
    <name>A0A9W9CLG8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aminopeptidase</fullName>
            <ecNumber evidence="11">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">N0V83_005643</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9 11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase M1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="11"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLG8"/>
    <dbReference type="OrthoDB" id="10031169at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000001">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000004">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF171">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="14">
        <location>
            <begin position="13"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="12">
        <location>
            <begin position="251"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="13">
        <location>
            <begin position="545"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4369879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="885" mass="99755" checksum="A383B8B354DBFF88" modified="2023-11-08" version="1">MASDRDILPAWVKPAHYTVSLHDIEFGGAFGYQGSVSITTNVNKDGGFHDLVLNAHQLKVHGAALKTGDKSWSVKDITYDEKRQRVTLDFGEKIDYSGNATLDVKFEGTINNIMAGFYRSKYTPKGEVPASVAKDDQFHYMFSTQFESCDARRAFPCFDEPNLKATFDVEIEVPKDQIALSNMPEKEIKPSKREGFHTVVFERTPIMSTYLLAWAIGDFEYVEAFTERKYSGKNIPVRVYTTRGLKEQGRFALDNCHKIVDYFSEVFQIDYPLPKVDLLAVHEFSHGAMENWGLITYRTTAVLFDPATSADSYRNRVAYVVAHELAHQWFGNLVTMDWWSELWLNEGFATWVGWFAIDHLYPEWNVWGQFVTDSVQQAFALDALRTSHPIEVPVYDGLEVDQIFDHISYLKGSSVIRMLSAHLGERVFLQGVADYLKAHQYSNATTNDLWSALSKASGQDVNSFMDFWVRKIGFPVVTVAEEPGQIGLRQQRFLLAGDVKSEEDQTTWWIPLGLHTGESASASSVHKTTALTQKEDTIRDVNEGFYQLNKNLTGFYRTNYPPDRLKKLGEARDQLTVQDRIGLIGDAYANAIAGYGSTAGLLALVERFQDEKDYLVWSQILTNIGNVRSVFSGSEDVSEALRKYHLKLITPAVEQVGWDFKEGEGFLLGQLRASLILSAGLVGHKATVDESLRRFDSYISGGDKNAIHPSLRRAIFATAIKQRGESAVKAIQNEYLSTTSIDGKEICLQSLGRVQTPELAEAIMSFIFSDKVAMQDKHSGTISLANNSKVRAEVWKFIRDNWDSKVHPTLSGNLVVLERFLRFGLNKFADEKIADEITAFFKDKDTRGYDKGLEVIDDTIRSYAGYKNRDGKVVSEWLTANGYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLR0</accession>
    <name>A0A9W9CLR0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small COPII coat GTPase SAR1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Small COPII coat GTPase sar1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">N0V83_006189</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPII-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 22">Belongs to the small GTPase superfamily. SAR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLR0"/>
    <dbReference type="OrthoDB" id="8300214at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00879">
        <property type="entry name" value="Sar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000161">
        <property type="entry name" value="Small COPII coat GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006687">
        <property type="entry name" value="Small_GTPase_SAR1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45684">
        <property type="entry name" value="RE74312P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51422">
        <property type="entry name" value="SAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10 22" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 22" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="13 22" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 20" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 22" id="KW-0653">Protein transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 22" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="299"/>
            <end position="306"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="401"/>
            <end position="404"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="445"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR606687-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR606687-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU003926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ4369105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="461" mass="50601" checksum="42F7EE30BA7CE946" modified="2023-11-08" version="1">MSTQLDAASIARVSLHDPKHFSIQHSQTLHRLVLLQHWNIPTGSKLLELGCGQGDCTTVLASAVGEQGTVVAVDPAELDYGAPYTLGQAQGHISQGPLGKRIVWVQKSPLDYLSSLLPSSTSSPPARESKAFDTAVLAHCLWYFPFPAVILSTFRALKQHSKRLLLAEWSLVATHPSAQPHVLAALTQAALECRKPESDSNVRTVLGPKRLTELALAAGWQLESETRVQGGEGLLDGQWEVSACLSSSFEREVEEHVSDEKERSVVLALRDACEASLEVWDVLSSLGLMNKHAKLLFLGLDNAGKTTLLHMLKNDRVAVLQPTLHPTSEELSIGNVKFTTFDLGGHAQARRLWRDYFPEVSGIVFLVDAKDHERLSESKAELDALLAMDELKTTPFVILGNKIDHPDAISEDQLRQALGLYQTTGKGKVPLEGIRPVEVFMCSVVMRQGYGEGIRWLSQYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLS9</accession>
    <name>A0A9W9CLS9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">40S ribosomal protein S30</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps30a</name>
        <name evidence="5" type="ORF">N0V83_006144</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLS9"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006846">
        <property type="entry name" value="Ribosomal_eS30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12650">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S30/UBIQUITIN-LIKE PROTEIN FUBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12650:SF15">
        <property type="entry name" value="RIBOSOMAL PROTEIN S30, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04758">
        <property type="entry name" value="Ribosomal_S30"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4369061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="81" mass="8949" checksum="11486042EC27EB06" modified="2023-11-08" version="1">MGKVHGSLARAGKVKSQTPKVSTHALPYTPTPARATYPTVEPQEKKKTPKGRAKKRLTYTRRFVNVTMTGGKRKMNPNPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMG7</accession>
    <name>A0A9W9CMG7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004941</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMG7"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF171">
        <property type="entry name" value="AMIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="1">
        <location>
            <begin position="109"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="578" mass="62194" checksum="36B2F8342A77D4E0" modified="2023-11-08" version="1">MSIVSLSTGSRSAFNIEHLKALAGRLNIDITDGQDADDYLHLLWSLEDVMKHIDEGPDYIHPSLKPQSVSKKRQFWRPRPEDNPLNAWSHRCDFSAAKPISDILARRTVAIKDNISVGGLPITLGAPALLLSELAEYRVSAIDATVVSRLLAAGAVIKGTSTCECFCASPLSFTSATGSVHNPHIHGFTSGGSSSGSCALVAAHAHASTQDEPIGETVELAIGSDQAGSVRIPASYCGVYGLKPTFGLIPYTGAASMSPMIDHLGPIASQLEDIAALLEVMAGYDDIDSRMTPESPLATQVKPYRQLLTGFRQQLASKPETGHTWRVGLLKESFLVPGISLDVCEIVRKVASQLFEAAGATVIDISVPMHLQGPIIWTASTRPSMSSWLYQGRPGGHLSYLPSHIQPRWPLSQENFNLLTSTNPALANIILSERFAHEYLPAGTEAKAHRKVFELREAYDRALKEVDILVTPCAPGVAMPHPKPVNAEGGPSSIMERLEAAIGVTSNTCPFNVTGHPAMNVPCGFSSPPSQPKARLPIGMQIVGRRWHDEEVIMAAALFEMGQQRVGSENLECAASRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMV1</accession>
    <name>A0A9W9CMV1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005291</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMV1"/>
    <dbReference type="OrthoDB" id="16535at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010591">
        <property type="entry name" value="ATP11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06644">
        <property type="entry name" value="ATP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="145" mass="16001" checksum="C8E6284547A16E54" modified="2023-11-08" version="1">MAFRSPTLRHALRQPLSNPRFLQRRWAQVQDIRFLATHGTQERILAKYKEKLESKARQEGVKDISELKERHKEKIEELRKQAIVPGATGPLTPPPSPQSSSSPSSSSPSPSTSPDAQPKSPGHHHHPSNSPILPSQPSTPPPPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMV2</accession>
    <name>A0A9W9CMV2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">L-lactate dehydrogenase</fullName>
            <ecNumber evidence="3 8">1.1.1.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_005111</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(S)-lactate + NAD(+) = pyruvate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23444"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LDH/MDH superfamily. LDH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.27" evidence="3 8"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMV2"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00300">
        <property type="entry name" value="LDH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018177">
        <property type="entry name" value="L-lactate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128">
        <property type="entry name" value="L-2-HYDROXYCARBOXYLATE DEHYDROGENASE (NAD(P)(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128:SF16">
        <property type="entry name" value="L-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00064">
        <property type="entry name" value="L_LDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0520">NAD</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="9">
        <location>
            <begin position="10"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="10">
        <location>
            <begin position="150"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="14"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="123"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4370590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="325" mass="33845" checksum="7AF67B9C096E500F" modified="2023-11-08" version="1">MTQPKLKTSIAILGCGDVGSTLAYTLILQPICSEVILVDPKKELLEAQVRDLGDATYRGASGVRVRAGTHKDAGQADIVVITAGAKQKTGESRLSLLSRNLNILESIFSSMAPISPHTILLLVANPVDILTYFARRLSGLPESQVLGTGTSLDSARLRGILAEKAEVAPSSIDAYVLGEHGDSQSLGPVQQSAPRPLALAMPDQLTPEFKASISSHTRGAAGAIIAAKGCTAYGIGNIAASICKYILFDSRSVRPLSFYQPELGCCLSMPAVVGRKGIIKAMPIQLDYEEQAALETCASGLRGVIEGGGEGVECGRAVTEGVGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNL6</accession>
    <name>A0A9W9CNL6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003738</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4371965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNL6"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF105">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="510" mass="56426" checksum="4106F7BA2A9AA830" modified="2023-11-08" version="1">MAASTPHKHPDAAGMYATAQDSAIHLQDAAESSQPRQPNPTHHGQTLHDPDYTTAGEDTTTGEDEVGEEAGEGEDEDEEDAQDFASDSGFDSGSLLGDETDTLASSILSHRMENGRQYHAYRDGAYWGPNDELAKEILDFAHHMYLLTLDQKLHLAPVESPQTILDCGTGTGIWAIDMADQYPSATVIGTDLSPIQPEWVPPNCHFEIDDVTLDWTFPPSHFDFIHIRELFGCIPDWDFFFAQAFEHTKPGGYIEIVEHSVQPTSDDGSMGPDHFYHTWGAVVIEMGRKFGKSFTIWEESAERIRNAGFVDVVVEEYKWPMNGWPQESKAKNIGRWNQLRLMDGVEGFMLRLLTQVGGWSVARAQLHLAQMRKELKSTKTHAYLPAPTIARQPISYSTPVAIPPPGSEIKNTPPENALASKPFKADVEESDVPKVFASDPASKPDEEVLVTGGIRELSRAPSVLIKALSPLRLTSTPEDAIWDPPQQSYVGKGKEAMRNPALRSVPPLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNP8</accession>
    <name>A0A9W9CNP8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_003843</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNP8"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04077">
        <property type="entry name" value="Peptidases_S8_PCSK9_ProteinaseK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000007">
        <property type="entry name" value="Subtilisin-like serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034193">
        <property type="entry name" value="PCSK9_ProteinaseK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF11">
        <property type="entry name" value="CEREVISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040987987" description="Peptidase S8/S53 domain-containing protein" evidence="7">
        <location>
            <begin position="21"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="8">
        <location>
            <begin position="156"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4372070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="471" mass="48792" checksum="3E26FD90D660E5BF" modified="2023-11-08" version="1">MRSFFTIAAGLLAACSTTLAAPQPKGDGFISKFNIPADHSNNTQEYIITFMRNETVPKGVEAYLEMMELKGDDVVKFVSTQNYTKIFILEMCQKHVDAFNALGEVASVEEKTTIKSFVTQKTGAPWGLQRISNEAGASGSPQGQDFTYSFDDTSLGAGVDIYIIDTGVRTTHAVFSGRATQGFTATGSFTDGDGHGTHVAGTAGGAKFGVAQGANIIEVKVLGDDGSGSSSDTIAGMDWVISNHEKRKTQPGFVGSIMSMSWGLQGTANSVDEVIAGASEAGIHVSVAAGNDGADACGSTPAHLGGSNSNVVTVGSVNIDNEISSFSNIGTCVDIYAPGEQILSAWSTGDNIINFLSGTSMACPHTTGVMAYLMAQDVAGLGQNPAALKAKLLATAREGKVSGSTGGSANLLLSNGVDGGVTAKRLVKNYVVPERSGLGGSPASRAAAAVSNDIVIDKRFELHSRDSKLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP10</accession>
    <name>A0A9W9CP10_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein transport protein SFT2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SFT2</name>
        <name evidence="10" type="ORF">N0V83_003161</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Nonessential protein required for the fusion of transport vesicles derived from the endocytic pathway with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Golgi apparatus membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the SFT2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP10"/>
    <dbReference type="OrthoDB" id="660759at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011691">
        <property type="entry name" value="Vesicle_transpt_SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137:SF36">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SFT2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4372870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="217" mass="23926" checksum="2A996EE3D34703AA" modified="2023-11-08" version="1">MASASFRDSMNSLGWSRREQEAPVNTSSPSLLGRLQNLNPFGEGGYVRLPITETNPGAPLPAPSRREEEEGWFALSRWDRLLVFGGLLIAALAMFATCIGLMFTPLFIVKPRKFAILWSMGSVLFLGAWGVLMGPIQYFQHLISGPRLPFTAAYFGSIALTLYFSLGLHSTTLTFIAAIAQLVCLLWYIVSYFPMGSTGLRFAARFGGNRVAAWMND</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPF9</accession>
    <name>A0A9W9CPF9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C6 finger domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003448</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPF9"/>
    <dbReference type="OrthoDB" id="5422841at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="241"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="400"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="489"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="277"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="404"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="592"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="604"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="646"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="656"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="666" mass="73215" checksum="30765A64E37B6E3C" modified="2023-11-08" version="1">MSMSNMDPHATGDLTMASHASHHHAPPVPPHLKRKRSPSQHDIHHASAPLPKTAKTHNHLQINYLARHLDEDLPLITNDDTLPNILSLLGDYQGVLDRHESMACNLGARPLGPILIKRFERLFDGPPRVLKSHGKDGTTVTWLDVVEFARNKPEQFQLGQMSEGVRVCQFYTKQCRVQISEEDFVLISSGIPQKMIPPQPIIEDEEKELGTIEILEKNLGQICQLADQVAARTRQLNHRLKGRKQAILDRRTTASPAPPVPRPSSPSNVALMNGGNPVPPKMGHVPPPQTSPSGGGFVAVNARHHESNGHGTSSTTRHDLLSKFHTVSERRPSSQPPNGTIELTRRPSVSAPHVISHPSTPVNNPSRPPPQVAEVPLPMVARTSHMNESELHSMMNSPVPIPNTPSNLLPANSQRASQPQEKDDGGPFKAEMVHRMEALAKGERILPPCDRCRRLHMDCLKNLTACMGCTKKHAKCSWKEVREAELRGNFSYPPATNSNPHSETSDLDSGDRASTGSPAAMMSPSRHGLTPSSSHIPPPPNSNNNNGQHTPLQHLQAPSHERPDVVPQQQPQQAPHYEMSHDARIPPTRNSPPDRDREHELQRSAEAQLQEAAQSSLAAHAHTQTPAAHVKASTEYPSQTQTQTQPQPPQKPSQPPQQSQNQTMVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPG7</accession>
    <name>A0A9W9CPG7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001919</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPG7"/>
    <dbReference type="OrthoDB" id="6359816at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4374841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="182" mass="20743" checksum="00EA82BF0AE9F4FD" modified="2023-11-08" version="1">MVYEKAIHFDDIDMYLMKHILDFMYSSHYTLRGGKDVPTTGYCDHTTAALRRGPINSSGIFKRKCPCSGKILSPSHLMSHIRIYTIADYFGMSDLKTYCRQGTQDVLHVYWDDEELDLADALEEAFISTPEGDCGIRSVLVDTLKEHPGLWVNEGDVQDWLDDNPKVREKVDPDPDTLVLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPI0</accession>
    <name>A0A9W9CPI0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP-dependent RNA helicase DBP9</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">ATP-dependent RNA helicase dbp9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">DBP9</name>
        <name evidence="21" type="ORF">N0V83_001964</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits and is required for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPI0"/>
    <dbReference type="OrthoDB" id="1191041at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17961">
        <property type="entry name" value="DEADc_DDX56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001046">
        <property type="entry name" value="Probable ATP-dependent RNA helicase ddx56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF21">
        <property type="entry name" value="DEAD-BOX HELICASE 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 21" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 21" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="20">
        <location>
            <begin position="47"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="78"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="260"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="352"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="578"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="47"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ4374886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="597" mass="66856" checksum="7E28BEAADFFF3C3B" modified="2023-11-08" version="1">MKRKLNEHDVPEGEAPQPSKRRPSDASQTESTSPAKSTKATTTVTAASFAEFELEPRLLRGIRDQKWSSPTAVQSRAIPLALQGRDILARSGTGTGKTAAYLLPLLHNTLRRKGKTSLILVPTKELALQITKVAKALSAHCGQDIRIQNIAGKESEVVTKAKLADAPDIVIATPARASANINNGALSVAELAHLVVDEGDLVMGYGFKEDLDQIAQNIPKGVQMFLMSATLNTEVESLGSLLCTDPVILKLDDLDKNSQKVKQYVIKCAEEEKFLMIYAMFKLQLIKGKSIVFVGDTDRSYRVKLFLEQFGIKSCVLNSELPLASRLHIVDEFNKNIYNILIASDETEILGSRQEKSESRPKKKAKKDKENKADSGVSRGIDFLNVSCVLNFDFPATYKSYFHRIGRTARAGKSGTAISFIIPKEKYRKHKSTTFAGCENDEEVLKKVEKHQEEGQKLENYNFDMKRLEPFRYRFGDALRSVTRIAIREARIKEIRLELSKSQKLSRYFEENPEALAHLRHDQTLNHPARIQPHLKHVPDYLLPGGRKPEDVGFVGLNMPKPDRRKYVKGRGRKVVRGRNGKVDPLKTFNARGKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPI5</accession>
    <name>A0A9W9CPI5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001871</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPI5"/>
    <dbReference type="OrthoDB" id="62798at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF286">
        <property type="entry name" value="5-PHOSPHATASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G07600)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="401"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4374795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="422" mass="46996" checksum="F99D335CAE02F67D" modified="2023-11-08" version="1">MFELYLLTFNCARTLVDPQSLSTSFFDALPKGAPLPDLVAISLQEVAPIAYSFLGGTYLKPYFDRLTATVHLAADLHSHGSETLEHVATRSLGMTALMIFATPKFAERIQWIQSAGAGVGFSNMGNKGAVAMRVGLSCPTSDDTLDLTFTAAHLAPMESNVDPRNKDWENIVRNLVFVNGDESAYSSSEERPLLGSTRDNPSDHNGLFTAGNHVFFYGDLNYRTHDSPPGEEEHKSYPQPTASESSPLHYSQLLKTDQLTRERKANRTLHSLQELPISFPPTYKYSTSHDSKSADDEKWDWSKHRYPSWCDRILFLPSRSSSKLEPQIYTALPVQKTSDHRPVALSLRVDDKPIPRDADDVRSKPPFPINPQWRARRDAARRWEVVVGVLSYLTLTSKGNAILITVVGAILGSLWLAGRFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPI6</accession>
    <name>A0A9W9CPI6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calcineurin subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Calcineurin regulatory subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Protein phosphatase 2B regulatory subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CNB1</name>
        <name evidence="11" type="ORF">N0V83_003541</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Composed of a catalytic subunit (A) and a regulatory subunit (B).</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the calcineurin regulatory subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPI6"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050801">
        <property type="term" value="P:monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000047">
        <property type="entry name" value="Calcineurin subunit B type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45942">
        <property type="entry name" value="PROTEIN PHOSPATASE 3 REGULATORY SUBUNIT B ALPHA ISOFORM TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="21"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="60"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="90"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="131"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4373247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="174" mass="19819" checksum="C74FBF83ADE55874" modified="2023-11-08" version="1">MGNQQSGLLDNIASGSNFNREEVDRLRKRFMKLDKDNSGTIERDEFLALPQISSNPLATRMIAIFDEDGGGDVDFQEFVSGLSAFSSRGNKEEKLRFAFRVYDIDRDGYISNGELFIVLKMMVGSNLKDQQLQQIVDKTIMEADLDRDGKISFEEFQKMVENTDISMSMTLDQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPX6</accession>
    <name>A0A9W9CPX6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002859</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPX6"/>
    <dbReference type="OrthoDB" id="5373017at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="523"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="551"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="582"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="679" mass="73889" checksum="44A9FA6C6105166D" modified="2023-11-08" version="1">MATNAPYYQMRAGQFINAANGTNATQPQFASEDDEYDLSAAAVVSPVEHHNSAMYSEQPHPQMLPDNTIEENHNLVELLEAATTARHAAQAMDLGGAIPASTGTQGRGKRKRVSSSPVGDASYLADENLSNKRRRVDVPTDPQLQASDSGLRGNSEHANVPPSTESLLSDARAAGVHSAAALFRRSSERTSRKYTRPPMSKLFMSLQLSPENFLQLQAQAKTYMLDPAHPERQNCVGNRGKGDTDMVKLRLFNCVRGFLDDEVGEQFFGEHVEKPGQREAIEAARALGEEKTPSAEARLTWPRDGNKIIGLVTPLMRRMVTNERQRQYAIETRKGGAKKKERDDSMEATAPHENGNQTHDMEQHSQSALDPSFDQPQQFSQLASPSTPNDARHKLSVPPKIHNLHSDRDATALALTGARSSSAVRVKLPTDGPAEHNLHHINIFLTLAPRNSRPAIKLDETRITSAPPQKHLAWYEWNDFLEHVATLLQRAKAKYLEVGERAVTEESRIGAESLRGLAAAANALSNEDTNGEDTAPRSVPPLESRQRIVGQRSQVLGSNTSIPSLPTRADVSFPEEPDSLASALSATNENPGPTTDIDTDVSLLPPYVIKTIGPDGWKVISNAKDWYDVLLEKAFAVWADGVCNVLVELVDLPAPVQTMAAATIQGPVDTGGGSGKTKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQG6</accession>
    <name>A0A9W9CQG6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001952</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQG6"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="81" mass="9685" checksum="13EA3524F64EB48B" modified="2023-11-08" version="1">MFFMLQTYLLSSFSVYRKRPVVLMCLLQEDEVATEEEDEEEREKEEEHEEPVMILAKRLVQPDEEDKENIPPRLKSARLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQG9</accession>
    <name>A0A9W9CQG9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Endonuclease III homolog</fullName>
            <ecNumber evidence="8">3.2.2.-</ecNumber>
            <ecNumber evidence="8">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <shortName evidence="8">DNA glycosylase/AP lyase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">NTH1_2</name>
        <name evidence="8" type="synonym">NTH1</name>
        <name evidence="11" type="ORF">N0V83_001504</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the Nth/MutY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="8"/>
    <dbReference type="EC" id="4.2.99.18" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQG9"/>
    <dbReference type="OrthoDB" id="2099276at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000703">
        <property type="term" value="F:oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1670.10:FF:000003">
        <property type="entry name" value="Endonuclease III homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000014">
        <property type="entry name" value="Endonuclease III homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03183">
        <property type="entry name" value="Endonuclease_III_Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004036">
        <property type="entry name" value="Endonuclease-III-like_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030841">
        <property type="entry name" value="NTH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286:SF1">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01155">
        <property type="entry name" value="ENDONUCLEASE_III_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="10">
        <location>
            <begin position="182"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="106"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4376221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="422" mass="46669" checksum="31D843CAD8AA6630" modified="2023-11-08" version="1">MRTSRISRDTSRILAASRTQRTLRQTRTAANITTRPELIESRASDDTENGENDVSGSELSSVPTESDSDDEVRKISRKRKRGQETPIVLKHEAQEVSIATIASPKKATKPKKARRTPAKKIKASDGSIKVEPPSNWQEIYALTREMRNETVAPVDTMGCESLADRTRTPRDQRFQTLVALMLSSQTKDTVTAVAMKGMQERMPGGFNLESVLALEPPALNAFINKVGFHNLKTKYIKQTAEILRDKWNSEIPDTIEGLTSLPGVGPKMAYLTLSAAWGRDEGIGVDVHVHRITNLWGWHKTQQPEQTRAALESWLPKDKWHDINNLLVGFGQTICLPVGRKCGNCKLADRGLCPSAVVAKNTKIKKEEATVKVEAPGGESIVHEQSLVAEVDDVKAEDVEGGMLDIEDIGKAPRVRTPRKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQI1</accession>
    <name>A0A9W9CQI1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">AMP-activated serine/threonine-protein kinase regulatory subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SNF4</name>
        <name evidence="6" type="ORF">N0V83_001549</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase gamma subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQI1"/>
    <dbReference type="OrthoDB" id="286637at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04618">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04641">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF35">
        <property type="entry name" value="LD22662P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="152"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="229"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="301"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4376266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="359" mass="40075" checksum="B35CCF15B0C297E1" modified="2023-11-08" version="1">MSGVDTGDIATSNLAVNDARPAFVSPRTLLEQSRQVAPAKIFTALDREQLDGLKTIRAFLKARTSYDVLPISYRLIVLDTALLVKKSLNILNQNGIVSAPLWDSKSSTFAGLLTTSDYINVIQYYWQNPDALARVDQFRLNSLRDIEKALGVKPIETISIHPDKPVYEACRKMLESRARRIPIVDSDDETRRTMVVSVITQYRILKFIAVNVKETQKLRKPLKDLNVGTYTDLATASMDTPVMDVIHMLVKKSISSVPIVDKAGTVLNVFEAVDVIALIKGGVYDDLNLTVGDALLKRSDDFPGIFTCSLNDNMSTIYDTIRRSRVHRFIVIDEDSKLQGVVTLSDVLEHTLLEGMEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR27</accession>
    <name>A0A9W9CR27_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7820 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001249</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR27"/>
    <dbReference type="OrthoDB" id="5384459at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056722">
        <property type="entry name" value="DUF7820"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42078">
        <property type="entry name" value="GLUCAN 1, 4-ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42078:SF1">
        <property type="entry name" value="GLUCAN 1, 4-ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25130">
        <property type="entry name" value="DUF7820"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7820" evidence="2">
        <location>
            <begin position="152"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="412" mass="45398" checksum="8454FE044747F6C3" modified="2023-11-08" version="1">MRLPRRPIASIQPLPSIHTAFPINYDLTDSSYSAGINSGTRLLRQIDDGIEVVPFERDPRSAPILSPDSEEKEVFVVSQKEIEQLEKPLPKLPGSIWQRMGIRQRILALLGVQFVILMTIGLALMAAKAPSSSDPPSRARATGGDSVTVDAMDTIQRGTFALPVQLPQQQSSACLARMNESVAWQCATDTEFQLNVLPSPANNTKMTMVTLSAPPSNDSIPYGHQVPDFPPVELVTLPNGDGSGDGPAYHFLTTYDRTVLLKEDDLQLEQEQRVQPLSQHHTFQIGESLWRCVFNETLIEGYIYVNQATTPESTMSGNSTKNISMTASIPKVPYVVKLVEQRMPNGKGPYCEKVKVQKDGSLSSGSGKVMLDLSESASELEASKSELVRSAKFRSREQTPTSNHCRCQWLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CS11</accession>
    <name>A0A9W9CS11_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome c oxidase assembly protein COX16, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Cytochrome c oxidase assembly protein cox16, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">COX16</name>
        <name evidence="12" type="ORF">N0V83_000476</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the assembly of the mitochondrial respiratory chain complex IV (CIV), also known as cytochrome c oxidase. May participate in merging the COX1 and COX2 assembly lines.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the COX16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CS11"/>
    <dbReference type="OrthoDB" id="5516033at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020164">
        <property type="entry name" value="Cyt_c_Oxase_assmbl_COX16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17130:SF14">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX16 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17130">
        <property type="entry name" value="MITOCHONDRIAL OUTER MEMBRANE PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14138">
        <property type="entry name" value="COX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4377648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="116" mass="13436" checksum="39D2EAD77EB0D73A" modified="2023-11-08" version="1">MPGPFTSKSFSATLPNSLAARYRKALQKHPFLLFGLPFISTIVAGSFMLTPATALRYERYDRKNQQITQEQAMGLRGERRKVNMKDEYYRLQAKDLEDWEQRRVKRLPGEPDGTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CS90</accession>
    <name>A0A9W9CS90_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_000718</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the intradiol ring-cleavage dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CS90"/>
    <dbReference type="OrthoDB" id="5238185at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018576">
        <property type="term" value="F:catechol 1,2-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009712">
        <property type="term" value="P:catechol-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03461">
        <property type="entry name" value="1_2-HQD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.130.10">
        <property type="entry name" value="Aromatic compound dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039390">
        <property type="entry name" value="1_2-HQD/HQD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007535">
        <property type="entry name" value="Catechol_dOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000627">
        <property type="entry name" value="Intradiol_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015889">
        <property type="entry name" value="Intradiol_dOase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050770">
        <property type="entry name" value="Intradiol_RC_Dioxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33711">
        <property type="entry name" value="DIOXYGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G02910)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33711:SF7">
        <property type="entry name" value="INTRADIOL RING-CLEAVAGE DIOXYGENASES DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00775">
        <property type="entry name" value="Dioxygenase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04444">
        <property type="entry name" value="Dioxygenase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49482">
        <property type="entry name" value="Aromatic compound dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Catechol dioxygenase N-terminal" evidence="8">
        <location>
            <begin position="36"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Intradiol ring-cleavage dioxygenases" evidence="7">
        <location>
            <begin position="133"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4377888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="335" mass="36931" checksum="2E07623B960D1F91" modified="2023-11-08" version="1">MASQGGPDPSAVPVLKDLTIENITENVHRINSQCADPRLKYVMERLVSHLHDFARETRLSFEEWMAGIKFLTEVGQICTDVRQEFILLSDIVGLSLLVDSIDHPKPPSSTEGTVLGPFHTHDASTLPSGTPISGDPAGTPLLVLCTVKSTSGAPISNVKVDVWETDSHGKYDVQYEDRGNKADGRAVIVTDEEGVFWFKAIVPVPYPIPNDGPVGKLLERLGRHCWRPSHMHFMFEKGGFDNLVTALYVRGSDYETSDAVFGVKESLIVGLETVTAQQAKQYDVKEGTKLLKYDFVLVTDEETKKLRHDEAVKAMKSLGREGMMVVNGLPVPDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XX75</accession>
    <name>A0A9W8XX75_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_010925</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XX75"/>
    <dbReference type="OrthoDB" id="3740062at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4361984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="250" mass="27919" checksum="F9A4EDD90670DAC0" modified="2023-11-08" version="1">MQRTKLPTGGYIATITTSATESANVQIQNTIDNMRRVPDGYDIRKYSDSARKELLAGGTIQIMSMSGTRVLEEVPKLALVVVSSVFREHLEHNPNATTITVFDPFINYAAVSTLLQWVNTITKSSGEFSVSVVRRPIAIGLIKIYHAAHHLGMERYVDRFSKTYKDGLIERIPITPECLVLERCSLTDCSLTDWINALGARLAYLRRVRKLHAVDCAKFQDVFSKCHKLAQAVRDADARLNAKRGGVLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXV0</accession>
    <name>A0A9W8XXV0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7918 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010747</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXV0"/>
    <dbReference type="OrthoDB" id="436496at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057678">
        <property type="entry name" value="DUF7918"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25534">
        <property type="entry name" value="DUF7918"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7918" evidence="2">
        <location>
            <begin position="41"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4361806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="368" mass="41776" checksum="62BDF173F87D9930" modified="2023-11-08" version="1">MPTYRSINIALHSQFDVETLPEYHPAARAEYIARGISGFTPEFISDSTSTCSVYIPVLPGSNFWISYSVSPPVPDEHYFLFKLYINGAHIVSWSTGKEERWKGKTMFGLFERPEEQERKSRVEKRVLCFAPPNQSDGEWNDMADVFDEHACIMIKVLRANGRKRVKREMEEYSKTQHARNDKGIHLVNAGRAEAKHPKRFYQFSLIDPIDQPFATFRYYYRTWDQLRDLGLLDEEWNRAGEENDLSIIEPNETEAHNTKQDNGSHVSNKELIASRPLPTVPRKSESSSSTAYRPHPPYPVDDCPVRTPSPVRSVREGEGTRGSKTRQEHTFSASSLMSMVACSLKRCRIRGSAAGNDAPGSDTARGAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXY1</accession>
    <name>A0A9W8XXY1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010887</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXY1"/>
    <dbReference type="OrthoDB" id="5420391at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42084:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PPK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42084">
        <property type="entry name" value="YALI0E26631P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="274"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="582" mass="61926" checksum="3250504A477AE55C" modified="2023-11-08" version="1">MSADLFAEFGADSSSSKQQQAGSSVKQGGNAASTFSFFDGLNTAPPSVTQSKLASSQQQFQSFSTAPIEATATEDNDDWGDFEGGSSFEPPSVKQDPFAFAPQQVSQTPQNPWNTASTAQDLFTIEPSHPSLPSQGTWQSAPIVKAKKSADPSVLFDAEEDLDDDDDFGDFEDTQVSKPTQPAAASGSSALVDLLGDLSVSQPQLANTRQKAEGSASRAPRTIFPAKKEPAAGFGVVSKAKNSPMKSASTVQQEDDAWDTFDDWEASIPTKAPAKGSSKAHTAKATTAAIPSVSPAPILSPATDDPQPGELPPTNVPPPGVLLSLFPSLFAEAQEKLFKPMAAQTLPTRNKMLAEKGTIVYLQGYLILADVAAHIVAGRKLRWKRDQHLSQGMRIGPASSRATSGMKLTGIDKGENMKEEREVSDVVRVWKEQVGRLRHVISGANQIKAGTLGSAPDLQETMPVKTLKQAEGGIPARQPCMLCGLKRDERVTASDLAVDDSFGEWWIDQVNMHRGRVDCSKRQKQDDCNTGGLTGQFALGADIKARCGMRMLLGKNVSAGRGGMSGRRTAVVAGNVSDKCCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYR3</accession>
    <name>A0A9W8XYR3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010556</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYR3"/>
    <dbReference type="OrthoDB" id="194443at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843:SF2">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="20"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="364" mass="41331" checksum="277CCF34AE3BBA5E" modified="2023-11-08" version="1">MPFIKGATTLGHTFRNFDQTIITVKVGGKDCSESFSCHRELLRRCSSYFHTCLEEKSGSPYKSSNTTLELDDTSPDVFDAFMLWLYTGFIPHHLIEGGKEIHPPTHAVNLNGSDSTASQEQSESDDVDTASWGVALETDDTAIDGQNGETASSPVQTIAEKVPGDSESERNTPLPPARNPTEKPEIPKETLCPCCSRPYKWRGLEGFLERRFISLYVFAHRYAIPKLRRAVILAWQNNDEILQVMPDHSNVIAAYESLPPDASLCRYFLDMYSVYWNPDRDDERTIALRSQLPQAFLFELVTMLARGERPKMEKEWCEFHEHESEEEKKACLDDLYKDAVASKALRRIKDSKGWKGLVLKKGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZK3</accession>
    <name>A0A9W8XZK3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZK3"/>
    <dbReference type="OrthoDB" id="1933717at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051468">
        <property type="entry name" value="Fungal_SecMetab_SDRs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43544:SF32">
        <property type="entry name" value="CHAIN DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G01530)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43544">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="28942" checksum="7BBD4D13168A6E58" modified="2023-11-08" version="1">MVNNSIVLITGGNTGIGFESVKALYASPQAHTILMGSRSVDKAKQAISTLQSEIADSKSEIVPLQIDIEDDTSIENAFKEVESKWGRVDSLVNNAGGAFDSIMLRDPSASGIREAWQKAYSVNVASTEVFTQQFAPLLLKSSNSRLLFITSGLSSLNTCAGGFMAKTMKASPAKGWPKPTTGPSMIAYRSSKTALNMMMLEWNRLLEVDGVKVFSISPGFLATNLGGAGPEHLKHAGAGDPSVGGTFIKDVVEGKRDNDAGKVIDKDGVQPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZT8</accession>
    <name>A0A9W8XZT8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZT8"/>
    <dbReference type="OrthoDB" id="42525at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009784">
        <property type="entry name" value="DUF1349"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35332:SF3">
        <property type="entry name" value="FUCOSE-SPECIFIC LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35332">
        <property type="entry name" value="REGULATION OF ENOLASE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07081">
        <property type="entry name" value="DUF1349"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="292" mass="31524" checksum="3CA5030223C95D9E" modified="2023-11-08" version="1">MPPASSSFTPLNIPSDVVPEWMGSFTLAAPPNTDLWRKPPSRDTSTAPILYTALRSPFIAAEVTVSADWELEWDQGGLVIFAGAPPGQIAAAAAVAVPEVPSVVNISLDDETPSSQSCPHSPNLESLDSSPTQSPAPEVARESGSGPQRERGPPPPYESPAPASKWVKVGLEFCNNACHATSVVANSDGADWSLSALPPHHERRLDLRVKIERIGYALWVWYEDEVVGWKKLREVTWFFWGVEDKAVRVGVYASRPANFGVSMYERRNGGGGGGQNAGARNLCVDFEGLEIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZW7</accession>
    <name>A0A9W8XZW7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent DNA helicase II subunit 1</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">ATP-dependent DNA helicase II subunit Ku70</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">KU70</name>
        <name evidence="23" type="ORF">N0V83_009777</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Single-stranded DNA-dependent ATP-dependent helicase. Involved in non-homologous end joining (NHEJ) DNA double strand break repair. DNA-binding is sequence-independent but has a high affinity to nicks in double-stranded DNA and to the ends of duplex DNA. Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection. Required also for telomere recombination to repair telomeric ends in the absence of telomerase. KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance. Interacts with telomeric repeats and subtelomeric sequences thereby controlling telomere length and protecting against subtelomeric rearrangement. Maintains telomeric chromatin, which is involved in silencing the expression of genes located at the telomere. Required for mating-type switching.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ku70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZW7"/>
    <dbReference type="OrthoDB" id="3249161at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043564">
        <property type="term" value="C:Ku70:Ku80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00788">
        <property type="entry name" value="KU70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01458">
        <property type="entry name" value="vWA_ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1600.10:FF:000004">
        <property type="entry name" value="ATP-dependent DNA helicase II subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000071">
        <property type="entry name" value="ATP-dependent DNA helicase II subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.970.10:FF:000003">
        <property type="entry name" value="ATP-dependent DNA helicase II subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.290.10:FF:000001">
        <property type="entry name" value="X-ray repair cross complementing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.970.10">
        <property type="entry name" value="Ku70, bridge and pillars"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006164">
        <property type="entry name" value="DNA_bd_Ku70/Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006165">
        <property type="entry name" value="Ku70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027388">
        <property type="entry name" value="Ku70_bridge/pillars_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047087">
        <property type="entry name" value="KU70_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005160">
        <property type="entry name" value="Ku_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005161">
        <property type="entry name" value="Ku_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00578">
        <property type="entry name" value="ku70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604">
        <property type="entry name" value="KU AUTOANTIGEN DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604:SF2">
        <property type="entry name" value="X-RAY REPAIR CROSS-COMPLEMENTING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02735">
        <property type="entry name" value="Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03730">
        <property type="entry name" value="Ku_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03731">
        <property type="entry name" value="Ku_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003033">
        <property type="entry name" value="Ku70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00559">
        <property type="entry name" value="Ku78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SPOC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 23" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="SAP" evidence="22">
        <location>
            <begin position="624"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="559"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="567"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="584"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with DNA; for 5'-deoxyribose-5-phosphate lyase activity" evidence="20">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR003033-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ4363482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="663" mass="74072" checksum="4484525009898143" modified="2023-11-08" version="1">MADPQHKRPGEEDENEDEDEDEAIDDSSYKTIKDAVLFAIDVSPTMLEQPPKSEDKKADRDSPTSAALKCAYQLMQQRIISNPNDMMGILLFGTEQTDLKDGDSTFQHCYLLADLDVPSAQDVKRLRDLVENNDEAEPILKPVKDGASIATVLFCANQIFTTKAPNFSSRRLFLVTDNDYPVKVKADKDTAVTRARDLYDLGCTIDLFPISQPNHSFDRSRFYDDLVYPTSPSDPDAPVAIPSTTKVAKSGEGISLLKQLISSINSKATPRRALFTLPLELAPDLRVGVKGYILIKRQEHAKSCYVWVGGEKPQIVASSTSHMADDTARVVEKTELRKAYKFGGDAITFTPEEITRIRQCFGDPVIRIIGFKPSSCVPLWANTNKATFIYPSEADFVGSTRVFSALQQKLLKSGKVALVWFIARRNASPTLCALVPAEEKTNEDGEQSMPPGLWLVSLPFADDIRQFPEQAESPLKTTDALTDKMRLIIEQLQLPKGIYDPVKYPNPDLQWFYRILQAMALEEELPVLPDDKTIPRFKQIDKRCGEYIEDYGKEFEEAFTQQQKSSLAHRGKPTNKKRTGTTGVDEKPSKRIKQEPKVKEDPGEGDDEGITDQQMAHLNNKGQISKQTVAVLKEFLGQRGIGTSGKKADLLERVQDFLEGKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y042</accession>
    <name>A0A9W8Y042_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_009982</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y042"/>
    <dbReference type="OrthoDB" id="47494at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47178:SF5">
        <property type="entry name" value="FAD-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47178">
        <property type="entry name" value="MONOOXYGENASE, FAD-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="6">
        <location>
            <begin position="7"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4363685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="409" mass="43646" checksum="89E91CDB7C82787D" modified="2023-11-08" version="1">MSAPKPILIAGGGLASLLVARSLHRSRIPFLVFERDASIVFRAQGYRLRLSDKGLESIETVLGPAGFQRFYDACGKTGGAGFAAIDPLTGETLGGSAPSVANEQLTSREGKIVGISRGDMRKLFMEGLEDNVRWNHQVKGYESTSSGVRLIFADGSKSEEGSMLIGGEGVKSEVARQLTNGAIKVYDLGSRGIHGQAPTTAFRGLGEGVFRLVDDTTQANGGKVFLMTNVRANDMHNPDINFGWTMGGSPGVIKAPNDKDTITGPVAAQIAKSLTSHWHERVKPLFDNMIESEAAFWKITCSSPQGVPEWPNDPRVTLVGDAVHAMTPAGGNGANTAVRDSALLGRLIAEAWERGHGDSWTGVTKGYEDEMRVYASAAVKESYGQATSSFGVELDLETTPTINEYVAPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y064</accession>
    <name>A0A9W8Y064_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y064"/>
    <dbReference type="OrthoDB" id="3658421at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4364488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="204" mass="22054" checksum="AE11155802AF82A0" modified="2023-11-08" version="1">MAAANLAPLPQPGLPIFPGFTAQKPCTFLMKGQDLWSNKASFLISHASPSGEAGAAFLEIREREKNQISFLTTGGGGDGDGREVMRIVKKCHSGWSKSKSGTEYHGLRGDGTEAWCLKLHSGWSGTDFAGHDIQVQNKILGQEKGILMDGNPVATMSRHEAWSHMARHDLIHVAPGMDILLALGVNWVRADKQKRDTQAVVALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0D5</accession>
    <name>A0A9W8Y0D5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0D5"/>
    <dbReference type="OrthoDB" id="2115692at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052523">
        <property type="entry name" value="Trichothecene_AcTrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791">
        <property type="entry name" value="GNAT FAMILY ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791:SF16">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="144"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="242" mass="27531" checksum="9DBF7972F56030E7" modified="2023-11-08" version="1">MPLRYATPSDEAAMVSTCTAAFFDEPLFGPLMHPYRHKYPNDVKIFWHEKIRQEWKTPSNKIIVATTTEAGNELVVGVAVWQRQGDDEGAKRVEKEWVDIGPDAFPPLPSTLNRALDPSTRTILEDSYPYFSHHWNPSTNSIPRSRNYYLSLCCVHPLYERRGFGYELVRWGLDKARDESVHASVLASYNNDPFYLRCGFDEVVANCTEGEGNPLSEVKGGSILFMWAKGPIEETNDISKRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0H7</accession>
    <name>A0A9W8Y0H7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thiamine thiazole synthase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Thiazole biosynthetic enzyme</fullName>
            <ecNumber evidence="6">2.4.2.60</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">THI4</name>
        <name evidence="7" type="ORF">N0V83_009234</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[ADP-thiazole synthase]-L-cysteine + glycine + NAD(+) = [ADP-thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-carboxylate + nicotinamide + 3 H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:55708"/>
            <dbReference type="Rhea" id="RHEA-COMP:14264"/>
            <dbReference type="Rhea" id="RHEA-COMP:14265"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:90873"/>
            <dbReference type="ChEBI" id="CHEBI:139151"/>
            <dbReference type="EC" id="2.4.2.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="6">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homooctamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">During the catalytic reaction, a sulfide is transferred from Cys-222 to a reaction intermediate, generating a dehydroalanine residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the THI4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.60" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0H7"/>
    <dbReference type="OrthoDB" id="410463at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160205">
        <property type="term" value="F:cysteine-dependent adenosine diphosphate thiazole synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03158">
        <property type="entry name" value="THI4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027495">
        <property type="entry name" value="Sti35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002922">
        <property type="entry name" value="Thi4_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00292">
        <property type="entry name" value="sulfide-dependent adenosine diphosphate thiazole synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422:SF3">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01946">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="111"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="301"/>
            <end position="303"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="2,3-didehydroalanine (Cys)" evidence="6">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4364638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="333" mass="35456" checksum="A4093776BF9488CB" modified="2023-11-08" version="1">MSPPAATYDIPVASNGNGIGNGLSVKKNLAVKPAANGLAKTLDEMENNWEAFTFAPIRESQVSRAMTRRYFADLDNYAESDVVIIGAGSCGLSAAYTLASKRPDLKIAMIEASVSPGGGAWLGGQLFSAMVMRKPAELFLNEIGVPYEDEGDFVVVKHAALFTSTLLSKVLQFPNVKLFNATAVEDLITRPAPTAADPNAVRIAGVVTNWTLVSMHHDDQSCMDPNTINAPLVISTTGHDGPFGAFSVKRLVSMQQLEKLGGMRGLDMRTAEDAIVKRTREIVPGLIVGGMELSEVDGANRMGPTFGAMALSGVKAAEEAVGVWDERKAQNDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0P0</accession>
    <name>A0A9W8Y0P0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Putative transcription factor kapC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_010061</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the bZIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0P0"/>
    <dbReference type="OrthoDB" id="3892274at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF11">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="10">
        <location>
            <begin position="119"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="77"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="177"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="106"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="178"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4362944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="225" mass="25258" checksum="F1E886E1127E8A08" modified="2023-11-08" version="1">MDSQSFNYWMAQGNYIAPPHQTTVFEMPPELSKVPSLSGSPVSSTYDQFPAPIHQAYYPHIVDEPMQFGSYPVAMTPSDFGSDHDSPYCSPRPPPHAEPVFTSAHSSTPSERTPTTSGRRRAQNRAAQRAFRERKEKHARDLETRLSVLSDKYNKLEVSHSELNAAYDKLRKTIELLTQDDDAEGEDEDGTTTRRRSSSSDTLRKLLEILHGEFKGVTPGKSESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0T8</accession>
    <name>A0A9W8Y0T8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Essential protein Yae1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008618</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LTO1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0T8"/>
    <dbReference type="OrthoDB" id="48036at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019191">
        <property type="entry name" value="Essential_protein_Yae1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052436">
        <property type="entry name" value="LTO1_adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28532">
        <property type="entry name" value="GEO13458P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28532:SF1">
        <property type="entry name" value="ORAL CANCER OVEREXPRESSED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09811">
        <property type="entry name" value="Yae1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Essential protein Yae1 N-terminal" evidence="3">
        <location>
            <begin position="23"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="176"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="233" mass="25060" checksum="CEA2589BC9B654AF" modified="2023-11-08" version="1">MATQTDPFDTLLTLEDTLYTTAYTLGQSDGSHAGRIEGRVFGLEKGFEKFAALGALHARSVVWGARLPQLQQLQVLLPSQSESEIQNQTQPQPPANEKAATDRVIQRRGSGAQEEQDRSLTPLPSNPRLLNNITLLHSLTDPTTFSTDNTEEGVADFDDRYKRGCAKTKVIERIVNESSSSSDNSSSIAAKSPDGSPARGPVRVVGGEGGGEEGKKGKGDDSMEDFAGSRLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y107</accession>
    <name>A0A9W8Y107_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y107"/>
    <dbReference type="OrthoDB" id="3794059at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4363564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="150" mass="16925" checksum="F447D6E753141402" modified="2023-11-08" version="1">MSDQNTSPPLYAARHPSALTFSFSLSILQLGFKMSQKVFPPGSLNFKADIEHLLPLLPVKEGEVVRTDTLMEVIVQAARELGMEWWNHFRERAGVVFGVMHAGMIQRKWKEYRMDDGVSEALGSMELGMDEGAVFERLAEELEDALKVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y267</accession>
    <name>A0A9W8Y267_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">rRNA-processing protein FYV7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y267"/>
    <dbReference type="OrthoDB" id="2135053at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41805">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41805:SF1">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN FYV7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="22854" checksum="71495474648F85AE" modified="2023-11-08" version="1">MAPDDSARKAKKQKKGFSVGPANLPDGTHRRKVQKIKKDLIHKAKLKKEYAKLKARDDAEATTPRKSVYDRENAASDEDEEPAEPAPEPSLEPHPDRVKMLEEDEPEAIEEQSTVRRNRRLRPQPFKKETEIAQKKKEEAEERQRLRDAADKERTKKIAERERFRKTMAKARGGPNGQRKLGRESTVLLAKAQRLMGKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2B1</accession>
    <name>A0A9W8Y2B1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009546</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2B1"/>
    <dbReference type="OrthoDB" id="152248at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025649">
        <property type="entry name" value="DUF4360"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38847">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38847:SF1">
        <property type="entry name" value="PSEUDOURIDINE SYNTHASE RSUA_RLUA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14273">
        <property type="entry name" value="DUF4360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040799770" description="Secreted protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="22149" checksum="15D3B5171FBB22EC" modified="2023-11-08" version="1">MKWLLALLPLVAAAPALPDQNLGDAPPAGQVTIRGVSYGGTGCPQGTMSSQISSDRSVVTLIFDSYIASIGPGIAVTEQRKNCQLNVDLQYPGGFQYSILSADYRGYSAIQKGVSGTLKSTYYFSGQTAQTSTEYDFTGPANGDYLKHDEADSTSIVWSPCGASGMLNINSQVRLTSTNASATGLLTTDSTDLKFTQVVYVQWQKCTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2U1</accession>
    <name>A0A9W8Y2U1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HPP transmembrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_007870</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2U1"/>
    <dbReference type="OrthoDB" id="2016548at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007065">
        <property type="entry name" value="HPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058581">
        <property type="entry name" value="TM_HPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33741">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN DDB_G0269096-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33741:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN DDB_G0269096-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04982">
        <property type="entry name" value="TM_HPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="HPP transmembrane region" evidence="3">
        <location>
            <begin position="38"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4366234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="250" mass="27473" checksum="83D4C75C9A6C58D8" modified="2023-11-08" version="1">MSSPDPLVPVERFIQNGLSQLPPVVSRWLGYRGKNLPPSPTWVVCFWGFLGAFGGLSVILAIFGHTEYFRSRAVPPIVASFGASAILAYGAIDVPLAQPRSLIFGHFFSGLTGVIIATIFQFNLMEDPYPRLQWLAAALATAIALVVMHLTKTTHPPAGATALLPCIDQHIWALRWYYLPVLLLSSSIILVTALLTNNIQRQYPKFWFAVIPPPPAPKDDQHRHRKPPMAEDSSCGSPRSALRDLESGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y321</accession>
    <name>A0A9W8Y321_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nucleoporin Nup159/Nup146 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_008156</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="248320at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026054">
        <property type="entry name" value="Nucleoporin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039462">
        <property type="entry name" value="Nup159/Nup146_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23193">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23193:SF23">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16755">
        <property type="entry name" value="Beta-prop_NUP159_NUP214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117289">
        <property type="entry name" value="Nucleoporin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup159/Nup146 N-terminal" evidence="6">
        <location>
            <begin position="51"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="463"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1379"/>
            <end position="1474"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1228"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1303"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="463"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="509"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="539"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="603"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="619"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="690"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="705"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="920"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="931"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="971"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1004"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1031"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1071"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1126"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1385"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1417"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1443"/>
            <end position="1456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4365537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1505" mass="158526" checksum="ECB2CF4FB770274F" modified="2023-11-08" version="1">MSVGNAPTVEAGPEIEELETELLGFKALSQGGAGPKKVKILPEPWPTDKVPPATASLLSVASKPGFLAAAGPGVLVLASTEKVRKAYEQEAGEGDVVSDFTPDVRLPVPTLRHVVFSTDGDFLVISAEDGGLAVFDAVAVMNGNKKDPDVQIDTDRVAARALLPNPQPNLAHYFAIVLESGRLAIANVAEGSLKTIKSEGVTSVAWSNKGKAVVAGLQDGTAAIYLTTGDLKGIVPCPSEVDQNYTISGVSWLNTEEFLVVHSLKTPDVEEPPQDSKYHIVKSNKGWTSFTFHAFPYDPLLIAYDIPRRALPPRFSATRLRNWQPDLNDMLIITNSHTDTIAVLAESTKKLSPFQENADNFKLASVDDSRKAAVPRTSYGEDADSAFIGEALDLSSKEKILRPVPALEEEGINEAPWPLPAYMALTHEGVLAAWWVIWNKSVQAGTRYPGLIVGSEEDTTTASSAASVAAPSPKSAAPASTQPTSVFGQPPNASSSNGASSLFGKPTATFGTPSKPQFGSTGFGSPTPALMKPTPPGFGTPAFGSTATPGKPAQPAFGAPAFGSAAAPGKPAQPAFGAPAFGSAATPGKPAAPAFGAPSTIGTPGGSGFGAMGGMGSKPSPWGAPSQASQPQANPFAAAAGGSSGFAKFGQPGGSSTFSSFGSNNGSQSGFAGLGQGQKSAFGGLKTEPSFGSTVTVGSGTGSTLPSWANTPAQQSGSAIFGQDKSSFNTSSFESKESDMGDADEAERMQRDDATPTPQAPPPQQAEGLFGLAGGFKLGTTFSSDGTAKDDPAKPAAPANGSFFGSDFANTLGVESLKAPATPSKEEPPNVSTTPVSPPKQKSLFSSDTPAKESDTPKAAPPAKEKAPPMDDAPLPPDFITAKPSKVDDAPLPPDFINTKLPKSTEEDLPPLAGSPGIKVEAPSSSVEVSPIDDEDGDDEGFSEEEEEEDDEGDDEDEVEEPSPSGPATKPQPSKSGGWNFQDSVNQSPRVFPPAPTPPAIKSGATSQSGRSASPAQPALFGQPSKLPGNQSAFSGSSLFGQQQQKGSFPFGQSTTATKPHLPPPTNRPQASLRSPSPVRSSSTSALRARREPFVAPGAPLSASIQQSKPPTPQPQVSDLEDEEDERMREQLARPIEPSRELDEFVAYQNYTGKSHTKTGHAAQIEMIYKDINGMVDALGWNARSIKSFTQYHKQPQSGHKVDRQTLEDVEDEGQDGSWFEKFTLCEIDALRALEDELEQELDAGRVQDVLDKLAQLARLLHEKAKLMTRLNDIRRQIINRKDPEKAEALRKAPLSKELADGQKALRNQYARLLTSLQKAEEAASILRSRVVSHNAQNGRTGAVPTMDAVKKTINKLTALAERKNSDITLLESQLRKINITDPGNNNNASRPSSSSSRNAATPRRSRLRNSVLLDSPTAATTTTPTPRRSKITLAELNRRALTPDVDTPTATTPTPSKGYGLYYTPPEGSPTTRGYELARLGDLVDESIEALREKARRRRGLPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y453</accession>
    <name>A0A9W8Y453_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 16">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ARF1</name>
        <name evidence="17" type="ORF">N0V83_006935</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.</text>
    </comment>
    <comment type="function">
        <text evidence="16">GTP-binding protein involved in protein trafficking; modulates vesicle budding and uncoating within the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y453"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04150">
        <property type="entry name" value="Arf1_5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003500">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045872">
        <property type="entry name" value="Arf1-5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 13 15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10 16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 16" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 13 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 16" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU369003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4367354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="183" mass="20969" checksum="CDDC2ED59A2809FB" modified="2023-11-08" version="1">MGLTFSKLFDKLWGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNIQFTVWDVGGQDKIRPLWRHYFQNTQGIIFVVDSNDRDRVVEAREELQRMLNEDELRDAMLLVFANKQDLPNAMNAAEITDKLGLHSLRQRAWYIQSTCATSGDGLYEGLEWLSNSLRKAGHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4D6</accession>
    <name>A0A9W8Y4D6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_008082</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4D6"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045033">
        <property type="term" value="P:peroxisome inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000397">
        <property type="entry name" value="Serine/threonine-protein kinase KIN4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="172"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="112"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="519"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1088"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="612"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="648"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="706"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="754"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="804"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="819"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="837"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="888"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="934"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="946"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1007"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1030"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1040"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1088"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4366446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1110" mass="119099" checksum="80733A18CB02FFD6" modified="2023-11-08" version="1">MNNTATSMGSGPMPPPRTSSTRAQPSAATAGSSSQADRIRDDTANAAANAAQSRRRAKDSPRQQQGSSSRQPGHSRSATAVGQPPTGTSLPREESTVINRIVVADPQEDIARAQARQAEGVPSSSGADLTPITGLGLVGSEGVDDGGRGGGKSRQDHSKSEGSTKRSKFGNYILGQTLGEGEFGKVKMGWKRDSSVEVAIKLIRRETLGGNSNRLQKIYREIHILRGLDHPNIVRLHEMVETERHIGIILEYASGGELFDYILNHRYLKDGPARKLFSQLISGVGYLHRKGIVHRDLKLENLLLDRNKNIIITDFGFANTFSPTDELSEEIEYRLGDKDFIRSLGMDGTDSTRRGDLMQTSCGSPCYAAPELVVSDSLYTGRKVDVWSCGVILYAMLAGYLPFDDDPANPEGDNINLLYKYIVSTPLTFPEYVTPHARDLLKRILVPDPRKRADLFEVARHSWLADYSHVVGFITSSNTNATDAQQASVYSSELAHWLVYTSPHADLLLEDTFEQQPALARSASVREPGKPHATASPAHGGLTTKKEQINQPPSEKTKTTRDNKRRTVQVEYVAPQSQTARGEASPPAAETPKARVKEAPVPPTDGYQSSTSARPSRSAGGTGAAAPARPGREPQRSVSDYTAFGTAPVTSTGRPSTGGTLGPRLPSRGNSYGMPSVAAVAPTNAEGRFSQPKGQQYSISAPYAQGESSGAAQTSIGQPSTQRGQSIQLGPSGANAKGHRRSNTVTETLGRMTSMFSGRQPSYTQDPKESLTSQNSYANHPEEKKQKSYPPTSMAGPMSNEPISSGPRPSTESSRRTSFGFSRKNTGNSGETKPSRRFSLLPSSLSKTFSSHRESMPATTSYTERRGSSARPRAGSKPGMAFGRSGESRSPSQSTQGSNMPGFYDGQHDSSSRIRNANMSAAGPSSAPADRTQFDSYGNQATTLIGDEKFPNPRDPHPSMQNRPHRQQTDDSEADFAPRSSSQVPRNPYPQGMGGEDDDPYDSSSAQQQQQQYQQQQHQRKGAVLQKSRKFADAYDDHQSGANKGSSGSSKKTHGEAEDWYWWGPVVRLSGFAAEEGEYSCVFVGGGGGGGGGGGGGGAAAAADAGEEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4H2</accession>
    <name>A0A9W8Y4H2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_007292</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4H2"/>
    <dbReference type="OrthoDB" id="415825at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF39">
        <property type="entry name" value="FAD-BINDING PCMH-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4366767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="371" mass="40524" checksum="6EB8A212317587C8" modified="2023-11-08" version="1">MIDISQLNNVTVADDKKTAVIGGGAKISETIAAADAAGVLIMTGNCNGVSTLGALLGGGYGNTMGMFGFGVDSILEMRVVTADGSTRVVSSTKEKDLFWAMRGAGPNFGIVTSATVKAYATSEDERSAWCGALVFTPDKLEQVVQAVQDLELSSHEVTFLYFASSGPPAHAPVVIVSLWMYRGTPETGKVTFKSLYDIGPVVDTTRVAPYTAWNAAGDPFCAHSKRKPSFTAGLDRLDTKAWRATWDSFVDFQKKPGAQESLILLETYPMNEVRFAGESSAAFPHRKTRFQVIVMAWYDDPSLDDEAVEFGKGIRSLWQGSDGAEKKATYINFGHGDEPLEEIYGDNLAHLRELKREWDPENRFNQWFNIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4N4</accession>
    <name>A0A9W8Y4N4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase ROK1</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP-dependent RNA helicase rok1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ROK1</name>
        <name evidence="16" type="ORF">N0V83_008002</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with the U3 snoRNA and is associated with the 90S and 40S pre-ribosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DEAD box helicase family. DDX52/ROK1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4N4"/>
    <dbReference type="OrthoDB" id="360161at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17957">
        <property type="entry name" value="DEADc_DDX52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044764">
        <property type="entry name" value="DDX52/Rok1_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF15">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="242"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="506"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="145"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="325"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="679"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="162"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="325"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="342"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="683"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="744"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="757"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4366366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="767" mass="83550" checksum="5D61B1E5892D180E" modified="2023-11-08" version="1">MNVFKLLSRSSNAAQSSPAQKLPSSGAVANPQLFGHDEPSQLAQSANKSRKRKRGLITAESPPSPAALPADLDFFGGGAAGAEVNGDAISKKEKKRQRKEDLKEKDTLENGTEDIEEEPYHVDECKQILRSHKLKVAVLEDFAPQAEESRKEKKKSKKRKKEKEQKPQKKDAKRQLYPQPLTSFAQLRTRYGISRRLAENIMEQGYKLPTEVQLGALPLLLPRKGYNEEQNGSTVDTESTSGAEYAGDIDLLTVAPTGSGKTIAFLIPIINALLIQSKSLDAEAGPRAVVLAPTRELASQIVNEARKLAKGTAIKATLMRKGMEVVERTETEPIEDKVDLPEASDDDEDASDSDDEGKATQQKKRTPGRAELVKAAILVATPLALLNSLKRRDNTIANLPSVSHLVLDEADVLLDPLFREQTLAVWNACSNPKLRAGLWSATMGSNIESLTLSTLNTRWEALSESISDMPARPPLIRLVVGLKDSAIPNISHQLTYAATEQGKLLGLRQLLHPTSVSSSDPKAQPTLRPPFLVFTQTIPRAIALHSELLYDIPPEAGGSSRIAVLHADLSSSARDSIMTRFRRGEVWVLITTDLLARGVDFRGLNGVVNYDVPNSAAAYVHRVGRTGRAGREGGVAVTFYTQDDIPYVKLIANVIRASEKLRGVKADEGSVKQWLLDALPTPSKRDRQQLKKRGVEARRSGSGKGDGAGEKGRNRISTKSGYVRKMEHNRRGAKAGSKRRAEMGVDDGGDDADEVDGSGGSDFEGFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4V6</accession>
    <name>A0A9W8Y4V6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exocyst complex component SEC15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SEC15</name>
        <name evidence="8" type="ORF">N0V83_007537</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the SEC15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4V6"/>
    <dbReference type="OrthoDB" id="10267033at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.30:FF:000004">
        <property type="entry name" value="Exocyst complex component SEC15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.670">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.30">
        <property type="entry name" value="Exocyst complex subunit Sec15 C-terminal domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007225">
        <property type="entry name" value="EXOC6/Sec15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046361">
        <property type="entry name" value="EXOC6/Sec15_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042045">
        <property type="entry name" value="EXOC6/Sec15_C_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048359">
        <property type="entry name" value="EXOC6_Sec15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042044">
        <property type="entry name" value="EXOC6PINT-1/Sec15/Tip20_C_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12702:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12702">
        <property type="entry name" value="SEC15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20651">
        <property type="entry name" value="EXOC6_Sec15_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04091">
        <property type="entry name" value="Sec15_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025007">
        <property type="entry name" value="Sec15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC6/Sec15 N-terminal" evidence="7">
        <location>
            <begin position="49"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex subunit EXOC6/Sec15 C-terminal" evidence="6">
        <location>
            <begin position="400"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR025007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4367007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="774" mass="88025" checksum="876CEEE75517A947" modified="2023-11-08" version="1">MPSAVDPDDDLWLAIKQISQSSVGSDYIDQLIPIMKDARYSNQLQVTFDRYAGDRESEIERICNANHQDFVSSVDSLLRIRDQTVQMSGEILQLNESIQESIEKLAEQKKALVDSRGVRQNINEATQALNKCLNVLRIANQVQDMLREKNYYAALRALDELQTIHLKEISRFKIANLIEKSIPQTQEQIREAVKNDLSTWLYRIRESAQFLGEVSFYYTDVRRTRNQERAETDPRFSKFKLNSAIELVADETDEFDVLNNEEAGNETDFSPLFEAMHINETLGKSDQFKSEYAADRRTQKDLIIPTKLNLLDEECGDLSSLLESIAGFAIIEKATVSKTTNLRSQADVDELWDSMCQSAIKLITGQLHTVENDELLLKIKGRVALFMLTMEKWGYSVSAMNDLLLTLFSKYSELLKQRFSDDFLEIVQTDDYMPMPINSHEEYDKVVSVSWYSPEKSREELTFPCVLPFSQMYPLCCIDIRNFLNQIYLFSDDYFQKSTIIDETLRTALDDLLCQKVCQSLIDRLSSQYPGQIVQILTNLEHFETACVELQDLLFQARSSPSATGPIILNATDKFAKAKQQASNRVFELVNSKIDDLIETAEYDWMASKPEIEPSTYMLELTRYLSNIMSSVLLALPTEIKEFIYFDALSHASTAILNLPLSEHVKRITPSSVATLATDTAFLSDFVSSLNNPILMENLDELTQTVALMGTDNSEEFFDIAQRNRKYGKVDQLKGAILLEKVQEGAQVAAQSPTTTKTGASERFGTLGSRFGIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y506</accession>
    <name>A0A9W8Y506_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40 repeat-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006859</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y506"/>
    <dbReference type="OrthoDB" id="239865at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051150">
        <property type="entry name" value="SWT21/TCAB1_mRNA_Telomere"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13211">
        <property type="entry name" value="TELOMERASE CAJAL BODY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13211:SF0">
        <property type="entry name" value="TELOMERASE CAJAL BODY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="526" mass="57476" checksum="06CC6274C0B6CF8C" modified="2023-11-08" version="1">MGSAGSSTSSLLVRWLASSKVAERKWLLKEKESEDPNDDQDDFDHAKREAEDLMGTDISRESDLETLREIDTDGSSSGSSSPSASASSSSDADNSNDQGRTEILPHQKHFTSCIYEAQLSPDGTCVFTHDERRKFSVYHIANDILAATETRPLKPYAEFTSVASPIWAFAAHPHFNAQDSSSSYVLISRRNAYITLHNALWDMSRNYDQEEAQALKSGPVDIFTPVTSYKLVDHQTEAVTAPLSLAYSHDGAFFYAGKQNGIAIFDVEHTYDPIQTIATIPSRRNKLKGGGCGFKGFITALALPPPNTSFSRGLLAAGSRTRHMGLYDAISGEVVTDFLLPGTQSGVNKHIDDMQGIMGDGVHNLKWSPCGKYLYVAERFTDVLQIYDVRNFSLALGYCKSRKAFTKAKLGFDIWHSPYDTEATSHDIWAGGTDGNIRVWKDPHTKEGAIEPDEELAFDNEDKLPIVSTLVHPSGSLAVAARGHLEVIDCVGDVEGDGLVSGGIERGGGLKPRYRERGSLDILGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y561</accession>
    <name>A0A9W8Y561_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006491</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y561"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="96" mass="10525" checksum="1B6416D1EF54EF4F" modified="2023-11-08" version="1">MAIYGEEAAILDDVLKRYKAAFNQAEFLETLEREGQPAATIVSNDASADDGKEDSDDDSDDDSNDNSNESAEYAVLENIISKHCVILTKDWSTTTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5C1</accession>
    <name>A0A9W8Y5C1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008533</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5C1"/>
    <dbReference type="OrthoDB" id="5405791at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="238" mass="26108" checksum="5CD290664E02B48C" modified="2023-11-08" version="1" fragment="single">MTGQPVGSAAQVDRHHLHSRRAQRTHKVGVYVPSAAASAHDGAGYGGEELNTFFEGDYTSNVPRTSDELRRPSLLPAATAAAALASLHNHRAEYDWDSDPDATSEPDSKSYRPRARFAPTSIEQQLSTEEPYYTSTSIQRELLPSSLGQSPPGRSSTLPPGNRSVKPNRPRKSSVGQNARKAKHERQKSRDHNRRMSYDRKAYSAEPATAAAIMGKRWEDLIDAAASATEEDSRDLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5G4</accession>
    <name>A0A9W8Y5G4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EthD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007780</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tpcK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5G4"/>
    <dbReference type="OrthoDB" id="3183782at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009799">
        <property type="entry name" value="EthD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07110">
        <property type="entry name" value="EthD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EthD" evidence="2">
        <location>
            <begin position="19"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="132" mass="15388" checksum="43A272EA35EA70EC" modified="2023-11-08" version="1">MGEPANLPFIRIQLCLKKKDEVTDEFFHDYWKGNHVKLALENKKFRDKVIRYNQFHTSPRFKEEANSFKIPVPEFDGIAEVWVKDIETWKEIVTDPDFVAAIAPDEAHFIKAPIHIMIGYDNTVIGEAVKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y629</accession>
    <name>A0A9W8Y629_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y629"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10622">
        <property type="entry name" value="HET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10622:SF12">
        <property type="entry name" value="HET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="22"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="637" mass="72738" checksum="F3D1940B9D2BE759" modified="2023-11-08" version="1">MRLLNVKTRRLEEFHGDTIPSYAILSHTWGEEEVTFQDMTSEDHKQKHGYRKIEGCCQAAAEQRILYVWVDSCCIDKSSSAELSEAINSMFKWYEASKVCFIYLEDLPAGLDPFTLDSAFRRSRWWTRGWTLQELLAPHHIIYFDSTWERVFTSEMGQELFKKIDLPVRSEEVMDRTERRPGDRIQTIIEQIRYGLITEITGIPGAVLAKELELSEVSAACKFAWASQRMTTRIEDKAYCLMGLLGVNMPLLYGEGEKAFVRLQEAVISSSDDISLLAWGFGLAWEDIEELGYDKILASSPTAFREYPKSNHRCVRRPPKTHSTVTGHGLHIELPLLLISTTTRVWIGIIEEGSAGALGDSGTTALVLRQKNVQDTNIFERARGCPALRIPNFHSHKRVFRAPISKMVYLQDGGTASTAVPLEDNFDPPRLLLSYLTFMRKSINRHQYTSELAIFIKSFRNVGYTLSSRYPPIDPDMVSMFAREAHKWELSDGQIQSHYESPFEWLLCPGPGNERFYFILANTKGHCVAVKVQIRFKPPYIHSYQVELSHCNFERSATALEYACDTGLRTEAAKQFRKELRWNSYVNLWSSNTTKIHVSACPSQQRLSKKNVSFAQCSLVWSGGWVSAADQRDKPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6M3</accession>
    <name>A0A9W8Y6M3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Dicer-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">dcl2</name>
        <name evidence="17" type="ORF">N0V83_006121</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6M3"/>
    <dbReference type="OrthoDB" id="416741at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050688">
        <property type="term" value="P:regulation of defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd18802">
        <property type="entry name" value="SF2_C_dicer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001669">
        <property type="entry name" value="Dicer-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000032">
        <property type="entry name" value="Dicer-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.380">
        <property type="entry name" value="Dicer dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038248">
        <property type="entry name" value="Dicer_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005034">
        <property type="entry name" value="Dicer_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950">
        <property type="entry name" value="DICER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950:SF37">
        <property type="entry name" value="ENDORIBONUCLEASE DICER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03368">
        <property type="entry name" value="Dicer_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="3" id="KW-0930">Antiviral protein</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="213"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="RNase III" evidence="15">
        <location>
            <begin position="707"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="domain" description="RNase III" evidence="15">
        <location>
            <begin position="887"/>
            <end position="1061"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4369039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1191" mass="134438" checksum="5EBBCBB5AE52FF89" modified="2023-11-08" version="1">MSDFFIRQAGDGKGALPKILGLSASPVKTAKATAKDLQQIEQNLHATAKTPKIHRSDLMRYVYKPELIQVHYHTELLNSTISRLLCALEDAYTHYDLMKDPFIMDLLKKQQEGYDISMKWQKTIISQKTYCHEQLRTLVSKAKDMAQELGTSAMEWYLHQCIVKFEKLVQNSDHQLLDWTVDEKRHLLSILERLPSSSVTHGPLMTLDNISSKVTALIDIIVAEMGNNPEFTGLVFVEQRVWVAALAEILSVHPRTRDLFRVGSFIGTSQSSNRKTSIASLPEPKNQQTTLEDFRAGRMNLILATSVLEEGIDVSSCHLVIDFEAPKNLKSFVQRRGRARKQESKYFIFTADIGNTKSSGSWQSLEAQMKATYEDDMRRVEQAEACERESEDGERFFEVTRTGWRTERMARKDAAFEGYKALYHAGLVNDNLLPFRHEVDEEAAEFQTADYRPSMVPVSSTFDPWLSIARYNGDNPHLWYRTLLEVCTSEEQPMYMVLLMPGTIPALPELILHWNEAKRYTVKASWLPGTTLSDDEIQLLRSITWNILQSVLYVEKSTRDFLWLLAPCDPTGHITNIGALTDWHASTQHEQPALEHVKRGRLVPADWGLVTYQGRKYILKSLDVSRPERIDSLKDVQLHTTRLPKRRDFLHPVPDNQEKNDAYTKIEILQAAECTVACLPISYSIFALLLPSILYRLEVQMITDTARTTILKPISFEPPQVPILVTALTSSATGEEDNYEKLEFLGDTILKFVASVHLMAKNLNWPESYLTGKKGKIVSNAFLSRATLASGLDQFIITKRFTGAKWRARYISQVLADTTSPLKEERSSKVVADVIESLIGASYVVGGLPKAFVCMQTLLPLEDWTRISDANQVLFNAAPSQDEIYGFEIVERLIGYTFTRKSLLVEALTHASYTGPSAHCSYERLEFLGDAVLDYIVTTRLFNSGLSHHTMHLVRAAIVTASFLTYSMFETTVKEELTNKSTMQPEVHDRALWQFLRSGGHKLLVARDTALKQYMNVREQIAAALEHGATFPWHLLALVDAPKFLSDIVESVIGAVYVDSHGDISACEVFVRRLGLINCVDRILCDQVDVTHPKVRLGLLAVEKDVKYVQIKDEQGDDVSNSKLYRCRVKVGGTCVGGVVEGTTRLSTETIAAFQAVEILEHEADTVMDDIDDEDVFVDAEEGGGIMLENW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7U0</accession>
    <name>A0A9W8Y7U0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SUI1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005671</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7U0"/>
    <dbReference type="OrthoDB" id="199771at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11608">
        <property type="entry name" value="eIF2D_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000008">
        <property type="entry name" value="eukaryotic translation initiation factor 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039757">
        <property type="entry name" value="EIF2D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039759">
        <property type="entry name" value="eIF2D_SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048248">
        <property type="entry name" value="PUA_eIF2d-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058886">
        <property type="entry name" value="SWIB_eIF2D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057429">
        <property type="entry name" value="WH_eIF2D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12217">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12217:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26292">
        <property type="entry name" value="PUA_elF2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26291">
        <property type="entry name" value="SWIB_eIF2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25304">
        <property type="entry name" value="WHD_eIF2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUI1" evidence="2">
        <location>
            <begin position="557"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="650" mass="70078" checksum="96371D4FFA894E6B" modified="2023-11-08" version="1">MFKKKPTIKPLSPLKSSDRRRTADHIIADFALEVPPADADASPDDKAAAAAAATAGLTALRRSLLPENALSARFTTTAGPDLEHVSGTIYVGSHEGTGGHQRVLWVKIGDNLYPTVYTLWHNPNIVPLLYTPLAVVEKMQAGADLMTPGLQRGPPFPSKATKGAIVAIASLEAPTVPMVVGTCDIDVSALGKVQGTKGHAVSTVHWAGDELWSWSATSKPGSDPPLVIEGWDDDKQDGTGLAERTARMELDSEEGGVALDAQPSARSAAEEAQGLEGEAAPANHDFVDIVDDKELSTKEIDEAFKNAFLFGVRHHMDNNKDHPSYGLDFPLSQSHVMSQLVQPYLPTYTPARTASLQIKKSSWKNIRKFIKYLDKQSIVKSKDRDGHETVILDIDFKDRQIAQFVPYRLPKKETPTAASANGAPAATLTSESSVGQKLKLVSVLRPKEKLTPILQASNADPRGLYTPAELRQFLIAYAEAENLIDPKNKRLIRINPQIADALLGSSAADNAALTSGSMARDALAERIVSASSPFHAILRNDASIADTKPKAGSPPRIQITLETRSGNKTVSKVHGLEPYYIAPQPLADELRKTCAGSTSVDKLQGSSPKAPVMEVMVQGPQKEAILKALEKRGVNKNWVDVVDKTKGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7V1</accession>
    <name>A0A9W8Y7V1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable aspartic-type endopeptidase OPSB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Probable aspartic-type endopeptidase opsB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_005398</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7V1"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05474">
        <property type="entry name" value="SAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000011">
        <property type="entry name" value="Aspartic protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033876">
        <property type="entry name" value="SAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 10" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040902302" description="Probable aspartic-type endopeptidase OPSB" evidence="11">
        <location>
            <begin position="19"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="12">
        <location>
            <begin position="66"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="314"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4369636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="475" mass="49668" checksum="3B91017072493595" modified="2023-11-08" version="1">MKFSALVAAQLLSSLAAAVHLAPRKNPRAIGLDIQRRYVPDPVAHDRLRKRADTVQQTLDNLETLYYANASLGTPAQTFRLHIDTGSSDLWVNAKNSQLCQQGGNQCGDSGTYNANDSSTYEYVNDEFNISYADGTGASGDYATDTFRFGGQTVKELQFGIGYTSSSTEGILGIGYTINEAGVNYAGSKPYSNLPQKLVDDGTINTNAYSLWLNDLDASTGSILFGGVDTDKFHGELQTLPIIPESGVYAEFIISLTGLGMNNTNGSIFSNENVAVLLDSGSSLMYLPNNVVRSLYHTFDATYDASQGTALVDCSLAEQGGSLDFDFSGVTISVPINELVIVTDVSRGQPVCIIGIGPAGDSVSILGDTFLRSAYVVYDLANNEISLAQTNFNSTSENIQEIQSGSAGVPHATGVADAVSTAAVGTAAPRINGPSGTAGGSMSTSTAGAMPAFTASPWVSGAVVGAGVMLGFNGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8M1</accession>
    <name>A0A9W8Y8M1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquitin-activating enzyme E1 1</fullName>
            <ecNumber evidence="4">6.2.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">UBA1</name>
        <name evidence="14" type="ORF">N0V83_006753</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.45" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8M1"/>
    <dbReference type="OrthoDB" id="10252231at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004839">
        <property type="term" value="F:ubiquitin activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01491">
        <property type="entry name" value="Ube1_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01490">
        <property type="entry name" value="Ube1_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2660:FF:000001">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12550:FF:000001">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000015">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.180:FF:000001">
        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.60:FF:000002">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000001">
        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.180">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, FCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.80">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12550">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2660">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, SCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.60">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, UFD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032420">
        <property type="entry name" value="E1_4HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032418">
        <property type="entry name" value="E1_FCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042302">
        <property type="entry name" value="E1_FCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018965">
        <property type="entry name" value="Ub-activating_enz_E1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042449">
        <property type="entry name" value="Ub-E1_IAD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038252">
        <property type="entry name" value="UBA_E1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019572">
        <property type="entry name" value="UBA_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042063">
        <property type="entry name" value="Ubi_acti_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018075">
        <property type="entry name" value="UBQ-activ_enz_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033127">
        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000011">
        <property type="entry name" value="UBQ/SUMO-activ_enz_E1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01408">
        <property type="entry name" value="Ube1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF4">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16191">
        <property type="entry name" value="E1_4HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16190">
        <property type="entry name" value="E1_FCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09358">
        <property type="entry name" value="E1_UFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10585">
        <property type="entry name" value="UBA_E1_SCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01849">
        <property type="entry name" value="UBIQUITINACT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00985">
        <property type="entry name" value="UBA_e1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00865">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-activating enzyme E1 C-terminal" evidence="13">
        <location>
            <begin position="973"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="850"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10">
        <location>
            <position position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4368396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1106" mass="122623" checksum="E74EC74042CD981D" modified="2023-11-08" version="1">MAQQSSTAQTVTAVVNMVTAFVDMVTKLLELTKLLVPEWRQRLFQRADRFATAGKLGKRKHSGEDAGPNKKVMAENMQVDSPATAVEQLKEASGSNGEIDESLYSRQLYVLGHEAMKRMGSSNILVAGLRGLGVEIAKNIALAGVKSLTLYDPKPAALADLSSQFFLHPEDVGKGRAFVTVPRVSELNPYTPVQEFSGSDLTSDLSQLKQFQVVVLTDTSLEDQIKIADYCHENGIYVVITDTYGLFGTIFTDFGKNFTIGDPTGENVTNGIIASIDETGLVSALDETRHGLEDGDWVTFSEVEGMEGLNGCAPRKIEVKGPYTFSIGDVSGLGTYRKGGQYIQVKMPKIIDFEPFSKQLKKPELLISDFAKFDRPQQLHVGTQALHQFANVHKGDFPRPHHEADATELFKIAQEIASQGEEKVELDEKLIKELSYQARGDLSPVAAFFGGMAAQEVLKSVSGKFHPIVQFLYFDSLESIPTSTKRSEEQCAPIGSRYDGQIAVLGQEYQKKLGNVKEFLVGAGAIGCEMLKNWAMMGLGAGPEGRITVTDNDQIEKSNLNRQFLFRPADVGKLKSDAAAKAVQAMNPDLKGKIVTLQDKVGPETEHIFNETFWNELDAVTNALDNVEARTYVDRRCVFFRKPLLDSGTLGTKGNTQVVLPFITESYSSSQDPPEKSFPMCTLRSFPNRIEHTIAWARESFDSLFVKGPEVVNLYLTQPDYLGASLKQSGSEKQTLETLREFLVTEKPLSFDDCIIWARHQFEKSYNHQIAQLLYNFPKDSTTGSGQAFWSGPKRAPDPLKFDTSNPTHFTFVEAAAILHAFNYCIKPNATREHYVEVLNDMIVPDFKPDPSVKIQASDNDPDPNANQAGGEEPDSLDSIINQLPAPKSLAGFKLEPVEFEKDDDTNHHIDFITAASNLRAENYKIEVADRHKTKFIAGKIIPAIATTTALVTGLVNLELYKIIDGKTDIEQYKNGFINLALPFFGFSEPIASPKGTYQGHDGEVTIDKLWDRFEVDDITLKEFVDHFEKKGLSVQMISSGVSLLYASFYPPAKLKDRMGLTMSKLVEHVSKKAVPEHQKNVIFEITAEDQNEEDVEIPYVMVKVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8X8</accession>
    <name>A0A9W8Y8X8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein kinase ATG1</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Autophagy-related protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Serine/threonine-protein kinase atg1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">ATG1</name>
        <name evidence="23" type="ORF">N0V83_005302</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19 25">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 25">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer. Forms a ternary complex with ATG13 and ATG17.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Preautophagosomal structure membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8X8"/>
    <dbReference type="OrthoDB" id="346907at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14009">
        <property type="entry name" value="STKc_ATG1_ULK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000817">
        <property type="entry name" value="Serine/threonine-protein kinase ATG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000399">
        <property type="entry name" value="Serine/threonine-protein kinase atg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048941">
        <property type="entry name" value="ATG1-like_MIT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022708">
        <property type="entry name" value="Atg1-like_tMIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12063">
        <property type="entry name" value="ATG1-like_MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21127">
        <property type="entry name" value="ATG1-like_MIT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0072">Autophagy</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 23" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 23" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="30"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="352"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="542"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="819"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="905"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="947"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="433"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="547"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="563"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="581"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="819"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="953"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00030237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ4370780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="985" mass="109348" checksum="41E4534EDCDB7739" modified="2023-11-08" version="1">MATPSNPYAARRSGASPSSSTAPEQIIGKFKRMDHIGKGSFAEVYRGIHIEKRQSVAIKSVNMNKLNKKLKENLVSEISILKSLHHPHIVSLIDCHETPSRMHIIMEFCELGDLSAFIKKRADLVNHPQTQRMIEKYPNPAVGGLNEVIVRHFAKQMASALEFLRSKNYIHRDLKPQNLLLNPSSLYYSQSGTLERMPFAADANSLIPSTGIESLPMLKVADFGFARVLPSTSLAETLCGSPLYMAPEILRYEKYDAKADLWSVGTVLFEMMCARPPFRANNHVELLRKIEERKDHIRFPEGIISSRAMKNLIRALLKRKPIERMSYDGFFADPVIREDIPDLVAEDLPQAMQASELEPPVVEEPPRRVQKMPAEMDRRASESPYSRSPRDRTALGTTPPSRPSSRPVSGHFSSTPPKPVSLRRASNAPIEPIEEHVPQREQRRPTLTNAMTAPGRTATVQDPSMGATPIGHRRRFSRDDQAPAPSGLKEHLDRERIPQRAEANAMREAAERAAQDNALDEGFVFVEKRRVEIDALADEIAASPQTQRDRMINRESAMKRRSTTQGSPTSTTGATVPSKAIQIQQKPALTHQRTGSYERRRQYRPSFESATSALSKAMNMVNIRGLGLSPPVIKGLSPPQGYAAFPTYPTAQSSLMLVGEGGRTASKDEDSTVVKKIEELAQLSHVIYGFAEVKYRQLVPLAPSDQGLGIGPTGVARKTTSDVIEDDEDESDDDLTPETVLIISEEALVLYVKSLAMLNKAMDAAGNYWNRNRRGSASPDSVHRATTNANRLSQVVTWIRDRFNECCVKSEIVARKLKQAQQQLPSDHPSHPQNLSAASGSATTVGSADNILLTTGVTAEKLMYDRALEMSRQAAVDELTGLNLPNCDINYWTAIVMLEAILEDEDESSTNSRNRLDAEEINGLDGEDTQSIQKLLEQMRARHRVLKKKLEIQKTTTTQKRSSVTSAPQPHPSNRSSPSSQGTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8Z1</accession>
    <name>A0A9W8Y8Z1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005075</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8Z1"/>
    <dbReference type="OrthoDB" id="5420724at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42069">
        <property type="entry name" value="HYPHAL ANASTAMOSIS-8 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42069:SF1">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="152"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="233"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="299"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="469"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="505" mass="56079" checksum="6388B499E317BE7F" modified="2023-11-08" version="1">MAANDKKLTRITIERRDSKDSLDVKTPRTARFAEATSVYSPVDAPRSPFEYPTNHYKPQAQVSDVGFGYMQSVEMEETDRKYLPPPTPRTPAGGLKSALKSPGAPPRTPGGAMSILSPTFREEQQLEKREHFTEKEQAKDLKVKVRVRVAKIFLRGINFACSLIVLSMVATAFAIFNATKQLPKRNGLPAWADNTKVWPQILMLSIACISMFMSVVVMIAYCRGGHHRAEKVAAYYTVFAVGFFIFSIIMWAVGAAIFNQSKAQNQNKDMWGWSCVDNKRRHLFEDDVAYGLVCRLQNWALVCCIIEVVIEVLCITIYGIVFYRFWSKRKLRKSMMQRDRARSDLYFAQLRSQSAPNTPGFGPMSPRSGGWRPPPGHPMYVDPHSAAESGESDKVQYAYAREIAQPQPFTLGAPPIKVTGATPKIDQDGFESAPSRTNTASPPLPPVSPGFQERRNDHVGAAPGEQQYASVPIPGAYTPLASPSYPPPHQQAPGFDFGPAVTGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8Z7</accession>
    <name>A0A9W8Y8Z7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Hydroxyquinol 1,2-dioxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_004355</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the intradiol ring-cleavage dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8Z7"/>
    <dbReference type="OrthoDB" id="5238185at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018576">
        <property type="term" value="F:catechol 1,2-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009712">
        <property type="term" value="P:catechol-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03461">
        <property type="entry name" value="1_2-HQD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.130.10">
        <property type="entry name" value="Aromatic compound dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039390">
        <property type="entry name" value="1_2-HQD/HQD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007535">
        <property type="entry name" value="Catechol_dOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000627">
        <property type="entry name" value="Intradiol_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015889">
        <property type="entry name" value="Intradiol_dOase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050770">
        <property type="entry name" value="Intradiol_RC_Dioxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33711">
        <property type="entry name" value="DIOXYGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G02910)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33711:SF7">
        <property type="entry name" value="INTRADIOL RING-CLEAVAGE DIOXYGENASES DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00775">
        <property type="entry name" value="Dioxygenase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04444">
        <property type="entry name" value="Dioxygenase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49482">
        <property type="entry name" value="Aromatic compound dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Catechol dioxygenase N-terminal" evidence="8">
        <location>
            <begin position="31"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Intradiol ring-cleavage dioxygenases" evidence="7">
        <location>
            <begin position="110"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4371139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="35895" checksum="FBE1B46969F5C27C" modified="2023-11-08" version="1">MPNSAPLLPASLTIDNITSNVHAINSTCTSPRLKFLLERLVTHLHDFTRETRLSTSEWTSAIQFLTAVGQICSPVRQEFILLSDILGLSLLVDSIDHPKPAASTEGTVLGPFHTHDAPTYAANGATISHDEDGEPMLVACTVRDTRGNPIPGVKVDVWETDSKGFYDVQHADRAGPDGRAVVASDEHGEFHFKAIVPVPYPIPNDGPVGQLLEKLGRHPYRPSHFHFMFEKAGFDPLITALYVRGSPYERSDAVFGVKESLIVDLGLWGCGGVERYGVGRETKLMKYDFVLVGRRRSGVEASEGGGGAGGHEGEGEAEGIMVVDGMLVPDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9H2</accession>
    <name>A0A9W8Y9H2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9H2"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="108" mass="11731" checksum="708C916B3BE77922" modified="2023-11-08" version="1">MSRLDALLKGKKFGPRTASSAPAPQPSSNPETVDVSRERQIAELQKELQQLKNVNNNRVLAEDTLSEHAETLRNESATAEMIADGAKTTFDLTQAVPKALQDDTPASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA28</accession>
    <name>A0A9W8YA28_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_004055</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA28"/>
    <dbReference type="OrthoDB" id="5091764at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040888030" evidence="3">
        <location>
            <begin position="20"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4372281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="237" mass="23575" checksum="AA047F10B8DFCACF" modified="2023-11-08" version="1">MKTAATFLAATALASTSYASTVRLETTKCLNANIPLEQFDIEIGTSAPVAKDLPAVCGLRILSASDSIDVTTIQCQAFRDTEGTQPGSAVFTYASFANIATNPIQEKSILCTIPSAPSATVLGRRQDTNETTTSSAEDLPTSSAATSTPESTVLITSIASDTADSTSSVAPSTIVSTIVVSASSGLPTSSGNATTPSNTGRPSATQSGSGPAESTGAAGAIGIGAGVVVGAFAALFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA36</accession>
    <name>A0A9W8YA36_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="9">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_005115</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA36"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000037">
        <property type="entry name" value="alpha-1,2-Mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF89">
        <property type="entry name" value="ALPHA-1,2-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="10"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="280"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="393"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="430"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="516"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="350"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4370594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="526" mass="58903" checksum="1EDBF52ECCA93BA5" modified="2023-11-08" version="1">MPAPRRRQLQRSIQTLKGDSTGEPFQNTTPSNSKPQPRPAVQHAFGAASAEAKNEIDSRKEVVREVFKRCWKSYKDRAWTKDELAPISGRARDTFGSWGATLVDSLDTLWIMEMKQEFAEAVDAAVQIDFGPKIDGEINVFETIIRYLGGFIGAYDVSGCHDARLLNKAVEVADMAYASFDTPNRMPVSRWSPKKAVNGEEQLPAESVLIAEAASASVEFTRLSQLTGDMRYFDAISRVTDVLDKQQGLTKLPGMWPISVNMRKPDLTFDGFFGLGAMADSVYEYLPKMHQLLNGIGPVATQYHRMYEYAMSTAITHTLFRPMVHDKADILIAGANVDGKREDKGQHLVCFAGGMFALGGRLFENSTHIDIGRKISEGCAWTYKNAPNGIMPEVFSMTACPTLSACDYTSQPDSSPFSEVGDGRYVLRPEAIESIFYMYRITGDSKYQDIAWEMFQAISTHTQTEFANAAIGDVMKTPVEKYDSMESFWLAETLKYFYLIFSDPGEISLEDFVLNTEAHPFRIPYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA88</accession>
    <name>A0A9W8YA88_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_004750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA88"/>
    <dbReference type="OrthoDB" id="4424523at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4371531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="166" mass="19173" checksum="75182F172E6C61C5" modified="2023-11-08" version="1">MSTSAYRNIRDDLAVQKWGWVIYRCSYDDADDETRAQYRTCVEANSRGSIAQSDAPEIADRLEYFIKSDKEVLQNLPGFLEKGWSMNAFVKIVDADWEPSSTIAAGDQQIEEQDVFEPIDGCTEKDVGWMRIAPSMLNAEFYHALEGDEMAWDHQFYERPPTIVRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAI7</accession>
    <name>A0A9W8YAI7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">xylan 1,4-beta-xylosidase</fullName>
            <ecNumber evidence="11">3.2.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_003747</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Hydrolysis of (1-&gt;4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini.</text>
            <dbReference type="EC" id="3.2.1.37"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Glycan degradation; xylan degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.37" evidence="11"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4371974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAI7"/>
    <dbReference type="OrthoDB" id="10029320at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046556">
        <property type="term" value="F:alpha-L-arabinofuranosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009044">
        <property type="term" value="F:xylan 1,4-beta-xylosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031222">
        <property type="term" value="P:arabinan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11051">
        <property type="entry name" value="CYP59-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1700:FF:000009">
        <property type="entry name" value="Periplasmic beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044993">
        <property type="entry name" value="BXL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42721:SF3">
        <property type="entry name" value="BETA-D-XYLOSIDASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42721">
        <property type="entry name" value="SUGAR HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="12" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0858">Xylan degradation</keyword>
    <feature type="domain" description="Fibronectin type III-like" evidence="13">
        <location>
            <begin position="1161"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="460"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4371974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1279" mass="141990" checksum="F2B63C9DD4A28F5A" modified="2023-11-08" version="1">MAVLDLPWAMIALSSVVAALSYSIIRLINERRFYKDLPKPPHSLFWGHLKLLGETFKLLPKDCHYQAAVTTIARKYNMPEVFYLDLWPAAAGQVVVTNPDVALHMTVVRNHPKHEAEKWFIDPLIGAGNIVTTDGARWKYLHKMLSPAFSIMHISNMRPMVAAEVMKFRSILQKKVESGEKFRLEELTMNMTFDVIGTATFGRSLDAQTKGSIALKHFEDMCRAFMQSRESFNFVKNFFCNRKRDAARQKLDDVVAELIKERFEMLQREKVDLTGKRGLGIMDLILRDYLEEHRQTGRHELDPEFLASAITQVKTLLIAGTGTTSDTVCFATMLLSIHPEVVKKMREEHDRVFAPGIDATYEMLKSDPYKLNELEYTTNVIKEVLRFYPIGNTAREGIDTINFEGREYPTKNLMMCPVQFAMHMNPKIFPNASAFDPDRFVREDFPRHAWRPFERGPRGCLGQPLAMDELKITLLLITRDFDFTCADLKPNKTPRVEWTDLDMTFGDRAFQEFVFEARPRDGMPMTVKSSAPLERAKSLVGLYTLEEKINATSSGSPGVPRLGIPPYNWWSEGLHGIAGPYTNFSAKGDYSYSTSFPQPILMGAAFDDALITEVATVISTEARAFNNANRTGLDFWTPNINPFRDPRWGRGQETPGEDPYHLSSYVQALVQGLQGDASDPYKRVIATCKHFAGYDIEDWNGNLRYQVDAQISQQDLVEYYLVPFQACVQANVGAFMCSYNAVNGVPTCADPYLLQTILREHWGWNDTEQWITSDCDAVQNVYLPHQWSATREQAVADSLIAGTDLDCGTYMQEHLPAAFTQGLVNETALDQALVRQYSSLVRVGWFDSPADQPYRQLGWSSVATNASQQLARKAATEGIVLLKNNGVLPLSVDPSMTLGIFGDWANATTQLLGNYAGVPTFLHSPLWAAQQLNVTVNYAGGNPGGQGDPTTNNWLKIKPAVDTSDVLVYIGGISNSDEEEGHDRTSLQWSGAQLDVIGQLAETGKPTIVVVMGGGQIDSSPLVNNSNISAMLWAGYPGQDGGSAIMDILTGKAAPAGRLPQTQYPSSYVSEVPMSDMALRPGENNPGRTYKWYNGSAVYEFGHGLHYTNFSADITSELQDSYDISDLVSACKTSNEAYLERCPFTTVNVTVTNEGNVTSDYVALAYISGTFGPSPHPKKSLVAYKRLSSISSTSSATAMLNLTLGSLARVDEMGNKVLYPGDYSLLIDNGPLASVNFTLEGAQAMLDEWPQPPANRTGKGVSGFEGYFQAGFGSVQEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAT8</accession>
    <name>A0A9W8YAT8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">N0V83_005057</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the ZNF598/HEL2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAT8"/>
    <dbReference type="OrthoDB" id="3838338at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16615">
        <property type="entry name" value="RING-HC_ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057634">
        <property type="entry name" value="PAH_ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041888">
        <property type="entry name" value="RING-HC_ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056437">
        <property type="entry name" value="Znf-C2H2_ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044288">
        <property type="entry name" value="ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22938:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22938">
        <property type="entry name" value="ZINC FINGER PROTEIN 598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23202">
        <property type="entry name" value="PAH_ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25447">
        <property type="entry name" value="RING_ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23230">
        <property type="entry name" value="zf-C2H2_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="117"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="387"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="618"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="741"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="67"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="392"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="478"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="504"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="640"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="650"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="683"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="754"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="779"/>
            <end position="788"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4370536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="795" mass="86405" checksum="F1DD5F0240FFB039" modified="2023-11-08" version="1">MASTGELGASTAPQENHQVPANPQGRGGRGRGRGRGPHARSEEGAEHNGSRGRGRGGFPPRGRGRGHRDGAGGRGGHAPEKGKAPLVQTQENGQSLPALAKPNLQQSDNEDPDAEVCFICASPVQHTAVAPCNHRTCHICSIRMRALYKTKACAHCRTESDHVILTDSPEKNYEDFSTVDFFKSDDNLGIHFENPAVFDDTRLLLQYNCPDSECDVACLGWPDLHRHVKTAHGKVMCDLCTRNKKVFTHEHELFTFPELRKHQKFGDDNPGAIDQSGFKGHPECGFCRERFYGDDELYTHCRDKHERCHICDRRDNGTRKQQYYVNYDALEVHFRKDHFLCPDRECLEKKFVVFDSEMDLKAHQIETHPNGLSKDALRDARRVDMSGFQFRASHEQETRRDDRRRDGGRGRGRGRDPNTEPLPASSAQQLSRAELAFQRQVEAQNSRSATGRAFGGQLTAPTPGEAFAARPTPSAPEPATVTASRPSTTSASSVANGLGQLNLTSQPASTTPQTPQDQARQLRHNAVVERASTMLRNDEPKLSEFRTQVSAYRTSKLSATQLIDGFFTLFETNSKELGKLVKELADIFEISAKRESLLKAWNDWKAINEDYPTLPGSASGQTLNGGAAAHGGSRVLKLKSSTAQSSRSSANKQASWSTASSSNPFPALPAPSSGRIGAKPGQTPWASSSATTSARASPMPSRAAPPAKVNQKANLADAFPALPAAAKPTSTIFSPGYTGIGLRRDNSGRNTPVANAWGGASGANSGTTTPAVEDDGATGKKKGNKNKKQTLFQWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAU0</accession>
    <name>A0A9W8YAU0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase family T4 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S58 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAU0"/>
    <dbReference type="OrthoDB" id="2107894at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02253">
        <property type="entry name" value="DmpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.70.12:FF:000004">
        <property type="entry name" value="Beta-peptidyl aminopeptidase BapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.70.12">
        <property type="entry name" value="L-amino peptidase D-ALA esterase/amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005321">
        <property type="entry name" value="Peptidase_S58_DmpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36512:SF3">
        <property type="entry name" value="BLR5678 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36512">
        <property type="entry name" value="D-AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03576">
        <property type="entry name" value="Peptidase_S58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="DmpA/ArgJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="401" mass="43455" checksum="1FBE1F3579AE202E" modified="2023-11-08" version="1">MSTPLTTSSTPRQRIRDVLPTVWLGRYTPGPKNSITDIPGVLVSTQSIHTSDKYPNAPPKSINTGVTTILPRKEWFEKAAFAGMFRFNGSGEMTGSHWIEETGLLRSPIVITGSLGVGNAYNGIYEHGLRDHTDAEGKADWFLTPIVAETFDGFLHDITKFAVLPQHIVDGIDTASSEPVPEGNTGGGTGMICHWWKGGTGSSSRIIPAAGGKTFTLGALVQANYGKMWDFKIAGAPIGRIIYEEQERLFRANPTDPILFAQSKMLFKVAEAKDKKDGSIIVILATDAPLHPTQLQRVAKRATVGLSRVGGYGTNPSGDIFLAFSTGNSLAVERDDPWNAHTVDVQMVDDQTINGIFEAAADAVEEAIYNAVFKAETMDGNGNKIEAISIEKVKELMQKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB55</accession>
    <name>A0A9W8YB55_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_004063</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB55"/>
    <dbReference type="OrthoDB" id="48317at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003960">
        <property type="term" value="F:quinone reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106:SF13">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="3">
        <location>
            <begin position="26"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4372289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="366" mass="38913" checksum="EA7EFB0D7B9E7D3A" modified="2023-11-08" version="1">MSPSLSTPNTSPTPSQPGRWLVTKFGPPSVLAWKPHDFTASLSADKVLIRILVGGIAGVDNIQRAGGYPHPLARQPGFVTGYDLVGEILALGPSVPAESGLSVGDRVTALSMFGAHATHIVLPYDELIKIERTDDPLKICALPLNYMTAWGMLKHSGVHLLPGSSILIGSVSGGLGTAVAQLVAAFDLGIRMIGTCSPSKFDYVRSLGVEPIDRNAPDLVERVRELTNGEGVDVAYDGVCSEDSVQKSLTATKKEVGRVVVFGVMGQIAADGSGMVGDVQEVFAKRLQPPLVNFYRLDTEYPKNDGVKDLYAMVEKVRSGELNPVVAKLLRLSEGVKAHELLVEGSTIKGKLLFVVDADLAAQYGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBV2</accession>
    <name>A0A9W8YBV2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_003680</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBV2"/>
    <dbReference type="OrthoDB" id="3942336at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051061">
        <property type="entry name" value="Zinc_finger_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179:SF13">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="616"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="647"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="678"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="729"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="395"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="472"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="796"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="809"/>
            <end position="823"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4373385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="835" mass="93759" checksum="FDBE5A8774E5D1FE" modified="2023-11-08" version="1">MERFAITSHDSARLKSFTTQAVKENRLTTGTWRKRTWIGFVTLSRMIRAFLTHHIEHGAWNFDVIMSKCLSVVLISSLACRSGDIARSHGYKGMEYMQYKHIELYLPDDVGDEPQFHQLRAAVNIEFAKGAKQTLNENFIRYLRPLDDVESVHVCPIALLLVHALRNGLVHGTTVEEVLRHTLAQADRHVKWIYPDRPILTAIARKPSRCDLDVVTSPWQINDTVKQMGVLSGMLTRVHAHALRLGAIRDYAHMPKNTSNTIVSTDDLRRFAGHSHTAMTKGVTEKYVGDIASEYYNNRAAMGGVHHRREPKFAVDKQAVLRTKGPVTKDEMQADIDIHMPDKTIGDLTRNERTSVTHRVRRQRVETLATSAVAEPLSIRTSSNGKLKAAPALAQAKAKDVPSRKPKPTTSVARDVGSKDSAVGSSSSEDLVLLSTIDPALLDEESLAALHVPDANIDALQATIFGAANENEAGQDTETAPGLEGIATDPSSQDHGEDAARIVLGQGPEDTDRTVSNAVDWINSYAKYNVVNNAIFARNWLSFSEKGASFEESIGHHAMRGNSRDEPTPYMFHCQQTPGCTYSNIREYHVTQHEGICSELLVEAVKKAENTDEDVLRCTFPGCEYATKCGERLLQLHVRYKHNWEPRTCEECDDGTIYYTKWALSNHQQSVHSGRWPAKCTFPLCPDKQDFKSAGSMEYHLKRKHNLLTAESRIPYMPPRPEKTPWVTQTCVRSSCPSQSTFDRRSKMMRHLKSYHNMSIEEAEDLINNNAVTQAIIPEQRIVGPNRNAKRLRNRKIAAADKENVSPDPLDDAQYADEEEEDTGATKKMKKQRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC55</accession>
    <name>A0A9W8YC55_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_002769</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC55"/>
    <dbReference type="OrthoDB" id="4133832at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4374030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="299" mass="35515" checksum="413FB7D6CE3F805D" modified="2023-11-08" version="1">MSSLTSDSAIKMVDEQDGAVEGPVQEMEKLGLSDSKARKKGFLDLPGELRNEIYELVTYHRLPFLGFVHLLPLIHSCRQVRKELMPFCFSFCDKQIEFCRVQRFFEVFFLSSIPNFVPKQPEMYKVLACVPIEEEDHTCARRTPDLKWLLEFLIQYPKIPLLFSVSNDFYGGETFFYRLRDTVRSNPAWLKHIQDFESIEMWLEDRHPDRFMGGRVWYLDLVLKPEVAEPWWNDEEERSKQTQELLTELGLYEMCLPDEHSRYKSLVVNVGNGAYCRHSDYYNHHEHYLREHKWNPLGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCD8</accession>
    <name>A0A9W8YCD8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Delta(24)-sterol reductase</fullName>
            <ecNumber evidence="2">1.3.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_002595</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.72" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCD8"/>
    <dbReference type="OrthoDB" id="415825at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000246">
        <property type="term" value="F:Delta24(24-1) sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050614">
        <property type="term" value="F:Delta24-sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040165">
        <property type="entry name" value="Diminuto-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10801">
        <property type="entry name" value="24-DEHYDROCHOLESTEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10801:SF0">
        <property type="entry name" value="DELTA(24)-STEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4373856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="506" mass="57553" checksum="44C20C02F3D73E27" modified="2023-11-08" version="1">MSKSSAAVKGHAERVATISNKVRSFYEQRVPFRIFHGSSNSTRAAARQQVIDISGLNHVIAVDTTKMVAVVEPGIPMDKIVKILLAHNLMPAVVPEFPGITAGGAFAGTAAESSSFRYGYFDQSVIEVEIVLGDGEIVRASPSENADLFFGSAGAMGTLGITTQLEIRLIACGMFVELTYVPVFSHDEALQQFKGVGDDIDFLDAVMFSNRHGVVMKGKLLKERDTNVPIIKFTRARDPWFFTHVHKKIRCSPATGDYFCLTCHWTKNSHVYRQNNSTMTELVPVEDYVFRYDRGVFWMAAYGWAPNLWNRFTRFFLDFLWHTRFQYRVVHIVGGTPHIIQDLAIPEPNTGGFLQYLHNDFRIYPLWLCPIKHDPRPPMHTAQSNADPTKDLVNVGVWGSPNYGSDFLRAETFDQFIKCNRMIEEKVAEVGGLKWLYACNYYHESEFWRIYDKDRYVQLRLKWKAESLPNVWEKVKRSQQEVKQVSRGQVLKAFLSAGLGIDRLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCE9</accession>
    <name>A0A9W8YCE9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_002771</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCE9"/>
    <dbReference type="OrthoDB" id="4133832at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4374032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="27678" checksum="9B72ABA6D2D2E543" modified="2023-11-08" version="1">MDLRADAMGERSDIEAQPCFLGLPGELRNDIYEFLLLDQDPIRVTMPLISSCRQVRKEYMPLYFQRQTICLPIYRVQSFFETYFLTNDTKFIPPPICSVKVSIATDKVTAIDMKWLAGFLLQRPKISITFDNDVEYSLADDLKVLLDISKSNDVWRDGLTDYASVKLISRISKYPWEEDAEDLFWDWYLELVVKQGVLTNRPAYGSNSYLPDFGLYKLSGGPGNRAAPFAFLRIVLERQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCQ8</accession>
    <name>A0A9W8YCQ8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003924</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCQ8"/>
    <dbReference type="OrthoDB" id="5335351at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="167" mass="19227" checksum="95735F2490B12590" modified="2023-11-08" version="1">MDNKSAANKSEPPSHHDAEAIQPSLRRVSSEDTERPPSAQQLDRDRGEQADASDSDGLEDDENDPADKIADFDWDDLHERYHQAVDKCHGQETELMQEWETLMTFFRTWAQSGHQHETDRTYSRLRTRMTYVQHSEANLEKTRNHYISVVRAFESALNLLRANGLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDT6</accession>
    <name>A0A9W8YDT6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_003304</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDT6"/>
    <dbReference type="OrthoDB" id="256333at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08297">
        <property type="entry name" value="CAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="7">
        <location>
            <begin position="18"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4373013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="381" mass="40693" checksum="C45D6E5EA75199E1" modified="2023-11-08" version="1">MAPDLPASMQAQFLESYKTPYKLRTVPLPVPTTPHDVLIKVDAASYCHTDFVLAEGQMPGLPKSFPHIGCHEFAGTVISHSESPSEKANKLKVGQRVGVPGRAFHACETCFECKNNRPKDPVAEDDEGYSVHCVDAENNGLSRDGGFAEYAIVDARQVAPIPDSLTAVETAPLMCAGVTIYAALKRCGLQPGGRVGILGAGGGLGHLGLQYGVHMGYKVLGIEAADVPLTLAKDVAQRLDPQPRVVDARAEQASAIVQELGTQDGKTHVGEMGLDAVVVLPESQAAFDYGMKLLKTHGTCVVVSFPEKGFQISARDVVFRDIKILGSLVGSNKTLREMLDFSAQHKIYAIAKCYPLAGLNDLVEEYHKGGGGKLVVDMSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE52</accession>
    <name>A0A9W8YE52_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATP-dependent DNA helicase CHL1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-dependent DNA helicase chl1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Chromosome loss protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5 6">DNA 5'-3' helicase CHL1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CHL1_3</name>
        <name evidence="10" type="ORF">N0V83_002487</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">ATP-dependent DNA helicase important for chromosome transmission and normal cell cycle progression in G(2)/M. May have a role in changing DNA topology to allow the loading of proteins involved in maintaining sister chromatid cohesion in the vicinity of the centromeres. Has a specific role in chromosome segregation during meiosis II.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE52"/>
    <dbReference type="OrthoDB" id="267079at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034085">
        <property type="term" value="P:establishment of sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF41">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE DDX11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase-like DEXD box c2 type" evidence="9">
        <location>
            <begin position="3"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4375400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="305" mass="34856" checksum="AD584FCA9A011582" modified="2023-11-08" version="1">MVNSLTWLRDHKRRTFEEVFSVGAEDSNEPAWMLEHVQQQKRQEALRRKQEFNDRIAKIKAKEKRTKERYENGVPRFKRQKVAPGDANEDGDEAQFALDDYESDVESNKRAATGTFDESGFSAETQALMEKLGFSIGAPKEEVEAADETKIFFCSRTHSQLTQFSSELGRVKMRMAITPDDSADKTDENNLVEDVKYLTLGSRKNLCINDKVKRLGNATAINERCLELQSSTSTESRCPHMPTKESETLINEFRDYALAKIRDIEDLGSLGKKMGLCPYYASRPATKYCEVSDPSRPFIIYLLMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YED6</accession>
    <name>A0A9W8YED6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">P-type Ca(2+) transporter</fullName>
            <ecNumber evidence="2">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PMR1</name>
        <name evidence="16" type="ORF">N0V83_002223</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YED6"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000031">
        <property type="entry name" value="Calcium-transporting ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000040">
        <property type="entry name" value="Calcium-transporting ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000028">
        <property type="entry name" value="Calcium-transporting P-type ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00121">
        <property type="entry name" value="NAKATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="373"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="15">
        <location>
            <begin position="48"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="627"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="632"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4375139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1044" mass="112390" checksum="EDE2E72FCABC50C7" modified="2023-11-08" version="1">MSRASTPSLPLYHASAGTSKAARGASPSLQPSEPSQKRSATPSSITSAFSLLSPQETAQKLETSVTHGLSSSDASARVHVHGHNELPHEEPEPLWLRFIKQFKETLILLLLGSAAVSFIMGNLDDGVSITVAVTIVVTVGFVQEYRSEKSIEALNQLVPHSAHIIRAGVEHSRSSRAPNGTATEGIELENLKDTTASLAAAERRSTTISAILLVPGDLVLFHTGDRIPADIRITHAADLSIDESNLTGENEPVSKSPDTITPPPGIQRPSSPFYASEAAGTVGADIRLNDQKNIAFMGTLVRSGYGQGIVIGTGGKTEFGAISASLQEIESPRTPLQLSMDRLGKDLSYMSFGVIFFIGLVGLWRGWQFLEVFQIAVSLAVAAIPEGLPIIVTVTLALGVLRMSKRDAIVRRLPSVETLASVNVVCTDKTGTLTTNHMTVTKIWHFDEPNPIDVKKKHEIEDLDSPTRNILRIGNVVNNARLLSDQAASASTAAVLSSTLGDDNSLAKSRWVGQPTDVALLDLLDAFGEDDVRERLGARKYETPFSSERKWMGVVISGTSSGELTPGTDFAYIKGALERVLDRCDTYVTAQGKEVILDQARKQEALNAADSMAQEGLRVLGFASGASKGKNKNTSGTSTPAPFTKSELGDDEQYRGLVFAGLVGMNDPPRKGVERAIRRLMAGKVKVIMITGDAETTAVAIGKSLGMPITTNSALGRSVIRGDELDRMSEEELAQAIATTSIFARTSPEHKMKIIRALQSRGDVVAMTGDGVNDAPALKKADIGISMGRLGTDVAKEAADMILTDDNFATILNAIEEGKGIFYNIQNFLTFQLSTSAAALSLVLLSTFLGFQNPLNAMQILWINILMDGPPAQSLGVEPVDPAVMALPPRPRHARVLTRPLLQRVLQSATIIMLGTLTTYYLEMSDDNQVTARDTTMTFTCFVLFDMFNALTCRSSSKSVLAGEIGIFDNKMFNYAVAGSLVGQLMVIYFPPLQRVFQTEALGLLDLAHLLAVASCVFWADEGRKWYVRWKGRRGFGAGYSTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEJ4</accession>
    <name>A0A9W8YEJ4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Oxidoreductase ucpA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_002476</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEJ4"/>
    <dbReference type="OrthoDB" id="37659at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052178">
        <property type="entry name" value="Sec_Metab_Biosynth_SDR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618">
        <property type="entry name" value="7-ALPHA-HYDROXYSTEROID DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618:SF13">
        <property type="entry name" value="CHAIN DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17650)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4375389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="252" mass="26820" checksum="996EC47738640D9B" modified="2023-11-08" version="1">MAATKVALITASSAGLGAQIARAFAPDFRVVINYSSNADRAQSLVKELSSIPGSSSQASPRFHVIQGDMSSKSSVQSLVKETIDTMGRLDVVVSNAGWTRMTTFTDIEQQVNDEDWDKCFTMNVKTHLWLAYAAKDALAENEGTFISTASVAGVKPSGSSVPYAVTKAAQIHLAKSLAVILAPKIRVNSISPGMLLTEWGLKFPESKRTAAIGNTKLKRLATVEDVADQVRTLALSRSITGQNICIDGGSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEN1</accession>
    <name>A0A9W8YEN1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">GPN-loop GTPase 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_001115</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Small GTPase required for proper nuclear import of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEN1"/>
    <dbReference type="OrthoDB" id="5839at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17872">
        <property type="entry name" value="GPN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000552">
        <property type="entry name" value="GPN-loop GTPase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030228">
        <property type="entry name" value="Gpn3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF7">
        <property type="entry name" value="GPN-LOOP GTPASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4375838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="294" mass="32986" checksum="267DABBBC542464A" modified="2023-11-08" version="1">MSKFGTLVMGPAGAGKSTFCTALIQHLQNNKRPCFYVNLDPAAEEFAFEPDIDIKDLISLEDVMDEMSLGPNGGLIYCFEFLMENLDFLTDPLEEVTEDYLIVFDMPGQIELYTHVPILPNLVKVLMQGSLNLRMCAAYLLEATFVIDRPKFFSGTLSAMSAMMMLEMPHINILSKMDLVKGQVAKRDLKRFVHVDADLLDDDPSRNATNEQDSNKYRDPASKESLMSGSAFHKLNKAVAELIDGFSMVSFLKLDVQDEDSLGAVLSYIDDAIQFHEAQEPREPKEFDGDVEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YET5</accession>
    <name>A0A9W8YET5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Isochorismatase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_003609</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YET5"/>
    <dbReference type="OrthoDB" id="245563at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00431">
        <property type="entry name" value="cysteine_hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050272">
        <property type="entry name" value="Isochorismatase-like_hydrls"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540:SF15">
        <property type="entry name" value="BLR5631 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540">
        <property type="entry name" value="PEROXYUREIDOACRYLATE/UREIDOACRYLATE AMIDOHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="3">
        <location>
            <begin position="22"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4373315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="192" mass="20340" checksum="767C8CB83AFA4836" modified="2023-11-08" version="1">MAPQTLRSLLGAPAGTASPSDSALLIIDAQNEYASGALTVTNAAQSGKAIAELLEKYRAANAKIVHILHKTPEGAPVFTPGTNLADEFKELKAKDGETTIWKQFPGAFEQTNLQETLESWGVKKVVLTGYMAHVCVSTTAREASQKGYEVILVEDAIGDRDIPGVKGDEVARVALAELADAFGTVVKSSEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFE5</accession>
    <name>A0A9W8YFE5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Spc7 kinetochore protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_002686</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFE5"/>
    <dbReference type="OrthoDB" id="5592879at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990758">
        <property type="term" value="P:mitotic sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040850">
        <property type="entry name" value="Knl1_RWD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033338">
        <property type="entry name" value="Spc105/Spc7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013253">
        <property type="entry name" value="Spc7_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28260">
        <property type="entry name" value="SPINDLE POLE BODY COMPONENT SPC105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28260:SF1">
        <property type="entry name" value="SPINDLE POLE BODY COMPONENT SPC105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18210">
        <property type="entry name" value="Knl1_RWD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15402">
        <property type="entry name" value="MELT_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08317">
        <property type="entry name" value="Spc7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00787">
        <property type="entry name" value="Spc7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01315">
        <property type="entry name" value="Spc7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spc7 kinetochore protein" evidence="3">
        <location>
            <begin position="720"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="116"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="363"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="492"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="656"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="928"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="83"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="116"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="174"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="198"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="244"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="367"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="390"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="517"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="584"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="656"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="674"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="696"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="714"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4373947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1228" mass="135104" checksum="47A619E894152D10" modified="2023-11-08" version="1">MAQPDGKENIADGLIANTSFKAPALSPRKSSRKTRSKSIGPGGLGELEAPALKESSGNRRKSAFIPAVKSILSSNDDDEKKRREARRKSLAKRRVSFAPEATLHTWDVVEYLRDATSSSASSDATRRASNITQASTPASPAPASDPAEPPSTPPEQAEEPQPEPGSSPANQRAQHQKKNRRRSSGIPRMDFNNPDDIYSSSPLSGDNQSPGGVDNEGSVSEDDEDGTENLDTSPDTEDSSQSSAKLDAALRQAAAQAGTQKFDFEDEGEMTMEIAEDEITTSFKPWAKKSFVATYDQENVDPSPFSPAATVADDDDMSMDITRAVGRIVPQQQQADASSSDMDDDMSMELTMPLGSIQAMASNAPANRRKSLKRRVSMLEASQGSPAKRPASRRASLRQRRQSEDNQADDATMDLTMAIGAIKSAASPEAVTYPSLEGSYDDATMDFTLAVGSIKNNTATGADVFGSDVVDEDLSMEFTKIVGPGIKRADLQVATPEKPSPAIESVARPSKTPTPRKTPKKSPGRRRSSIVPGEVEPAPEVQVQATPMKSPAESRATLDPGTPEIVRQKDIEEVEPSPFVRRTPVSTSKTNGAVATPTSQRKQKAPMTIPEDPIAAPILNRRRSSLSAVQFSPLNAPREEPTLKSTSLLSNNIKLLSTPRKQTLMSPVKRGMTPKKSQTPQKQPTPKQKTPTPKKATPRKSMSPKKRVAFGAEPVEEEEVDNEASEVVSEVERISLQEFLDMTKIRFMDLSTTKRRHTAAPAAFHDVDVEEEEESLDRYVVAGACTVPEYELYQHACHEMKKYISDGRHFVRTMEANVLEENPLLFSEYLTAPPDQRIVMDNQFKNLKTNARLEARGEWYTWRSTLLQDLKSGLLHTLDGFKQDEINLNNQEQLLDVVLPPLIEKHELLSTECKQLQQRHDELNSCDREELEQTRERLVATDADLEEKRQLLEQLQEELAEKDARIEAVKERKVECVEEIKAAERVREECRGWSTTEVNDLKAKVTSLEQAHGWSITSASSTSITLTHLNDLELFFHPSAFASNSPNSPNASISLAYIGDTATPHPRPLTTSKRFFLQLIRAHLHCIPQSQTPISSLLSLVKNSWATALAVAEGIRCLEHSYITEEFILSDERMGVAANMLLPTVQTKVKVLFEVGVGGIGERGVETEVGVKAEVVYGEKYGEEKMGAFLRDFCGSEVRDVDGMSVWADGMCDLASRLRKTGRKGERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFJ3</accession>
    <name>A0A9W8YFJ3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycoside hydrolase family 125 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002032</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFJ3"/>
    <dbReference type="OrthoDB" id="7771656at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008313">
        <property type="entry name" value="GH125"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31047:SF1">
        <property type="entry name" value="DUF1237 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31047">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 157 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06824">
        <property type="entry name" value="Glyco_hydro_125"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028846">
        <property type="entry name" value="UCP028846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01149">
        <property type="entry name" value="DUF1237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040866980" description="Glycoside hydrolase family 125 protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="60219" checksum="268C98EBB39306C5" modified="2023-11-08" version="1">MPILRVSLLLSWILAFLLPLSLAQCPIYTSYAAQRHVPYTEGRYNLSYARPIPPCRTFYSAEVEATIERLRHVIVDPDLFRLFENSWPSTLDTTIAWRGVSNSTDDGGDDGEEGDFSEKGKREQEEEELTFVITGDIEAMWLRDSANQLQAYVSVLKANDSRDSLASLFRGAINLQARYILESPFCNAFQAPDESGMMRKSSMNSDQITPFFDYYKVFSCQWELDSVASFLQLSVDYFSATKDYQFFQRSSNWTKAVEVVLRTAESMMIDSYTEDGEWQKTPYTYCAPYGGTPINDCAGSPHKGNIGLVRSFHRPSDDACTYQYLIPSNMMFSAALNGSSHIMQRIEPRGGNLTSWMRQMSTGINRGIETHAVVSAGSGGGNKIYAYEIDGYGSANLMDDPNIPSLLSAPFLSYTTLHDPIYRNTRRRILSKSNPYFSWGPVISGVGSPHTLPGKVWPMALIMTILTSEDDVEILGNLVMLLRSTDGLGLMHESVDSRDAGRWSRQWFSWANGLFGVMVLELERRAPHLLEMSFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGR3</accession>
    <name>A0A9W8YGR3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000169</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGR3"/>
    <dbReference type="OrthoDB" id="5408618at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="135" mass="15930" checksum="77F7396DB4FDF3CB" modified="2023-11-08" version="1">MSKADYAREEPQRVTRTRSWDRAVVFDPKDNSVDIRKKKDMDKISSKLLQLNWDWNEGRHLLGNHRYQQLELPKGRKKVSLFMDQDDKMRLKTDEKRSGDDVFLARLNLSHLGLTNWALFSLGYLSTLYVSTDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGS9</accession>
    <name>A0A9W8YGS9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">ATP-binding cassette permease mdl1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">MDL1</name>
        <name evidence="15" type="ORF">N0V83_002066</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Mitochondrial peptide exporter (TC 3.A.1.212) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGS9"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18573">
        <property type="entry name" value="ABC_6TM_ABCB10_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000048">
        <property type="entry name" value="ATP-binding cassette sub-family B member 10, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000218">
        <property type="entry name" value="Multidrug ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF1">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002773">
        <property type="entry name" value="ABC_prm/ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="143"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="14">
        <location>
            <begin position="100"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="420"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="660"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="675"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4374987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="696" mass="75291" checksum="38BF5F874C0E15B8" modified="2023-11-08" version="1">MFHASRILAEATTKAPAKPRKSYRDEIKEKGFVDVEEHDGETDAEERGFVKSEKATQAKQINLSAKLSKDGTGAGSTPGGMKEIWRLIKIAKPEMGTLSWAFVFLIFGSAISLSVPFSIGRILDAATNADKTDGLLFGFSMTTFYFALAGVLTAGAAFNFGRIIILRIVGERIVARLRSQLYRKTFIQNAEFFDANRVGDLISRLGSDTIIVGKSITQNLSDGLRALVSGAAGFAMMGFVSIKLTGILALIAPPVALVAFFSGRQLRNLSRKIQKNLGTLTKIAEERLGNVRTSQAFAGEVQEASRYNRQIKRIFALGKQEAIVAASFYGSTGLMGNLTIIGILYVGGNMVKNGVISIGELSSFLMYTVYAGSSMVGLSGFYGEMMKGVGAASRLFELQDRNPTLSPTKGEPVKSARGPIEFKNVSFSYPTRPAVSIFKDLNFKIEQGTNVAIVAPSGAGKSTVASLLLRFYVPTSGTITIDGRDITTLNAKQLRRKIGFVGQEPVLFSGTIAENIAYGVPHATRAEIVAAARKANCQFISDFPDGLETHAGARGTQLSGGQKQRIAIARALIKKPDILILDEATSALDAESETLVNQALSKLLQENNTTISIAHRLSTIKRSDRIICIGADGNVAEEGTYEELTSNPDGAFNKLMEWQLNGGETDTRRSPQPTELEEIEQELEEETEEESSRNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHX2</accession>
    <name>A0A9W8YHX2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="9">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GAS5</name>
        <name evidence="12" type="ORF">N0V83_001452</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Lipid-anchor</topology>
            <topology evidence="1 9">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHX2"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000032">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF5">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 9" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041012324" description="1,3-beta-glucanosyltransferase" evidence="9">
        <location>
            <begin position="17"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="337"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4376171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="451" mass="47664" checksum="91ED9B2BA6F2A5ED" modified="2023-11-08" version="1">MKTFAIAAALAVSAAASPTATPQKRASITPVTVKGNAFFAGSSRFYIRGVDYQPGGSSDAKDPISDATGCKRDIAKFKDLGINTVRVYTIDNSASHDECMNALADAGIYLALDINTPKYSINRANPQKSYNKDYLQSIFATVEMFAKYDNTLLFFSANEVINDDATTNTAPYVKAVTRDIKSYINARGLRKIPVGYSAADVESNRYEMAAYMNCGADAIRSDFFAFNDYSWCDPSSYKTSGWDQKVAKFGNYSIPIFLSEYGCNKNTRKFEEVASLYGTDMTGVYSGGLVYEYSQEDSNYGLVELDGSSVTERSDYTALKTAYAGTKNPTGDGGYKSSGSASECPSESSTWNVTMATDKLPAIPDGASDLFKNGCGDGPGLKGSGSQDSGTDKVTLADASSGAVTSGGTSPSGTASKGAAASLRPGEFSMAPLVCGAIVLVSSLLGGSLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHX3</accession>
    <name>A0A9W8YHX3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EthD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_000668</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tpcK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHX3"/>
    <dbReference type="OrthoDB" id="2519291at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009799">
        <property type="entry name" value="EthD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07110">
        <property type="entry name" value="EthD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EthD" evidence="3">
        <location>
            <begin position="2"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="91"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4377838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="122" mass="13496" checksum="1F16C1BB6C1FA502" modified="2023-11-08" version="1">MTRRYLARINRKGFGGPANPDRPPLTLKGEMQGLDCDCIAEMNFDSEAIFQDFYDKRHEKDIAAVVLEDEKAFLEPGKTTIVVIGETWSTDQNGVSTGEVNYVSKDDRVDGGESPSEGSYVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YID4</accession>
    <name>A0A9W8YID4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">propanoyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.176</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Propanoyl-CoA C-acyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_001694</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.176" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YID4"/>
    <dbReference type="OrthoDB" id="542135at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006102">
        <property type="entry name" value="PRK08256.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF1">
        <property type="entry name" value="NON-SPECIFIC LIPID-TRANSFER PROTEIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="7">
        <location>
            <begin position="53"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="8">
        <location>
            <begin position="246"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4376411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="442" mass="48185" checksum="C34BDF15806CEE99" modified="2023-11-08" version="1">MPAKNNIPAYVLGVGLTKFIKPRKERAYPEMGYEAGVKAMLDAQINYDDRIFYQFGMSTIPIYNTNNACATGSTGLHLARTLIKNGAIDCALVIGFEQMQPGSIKSVWSDRPSPMGLSMRMMADTRGVDKSPPNAQYFANAGKEYMEKYGAEARDFAEIARISHLHSSKNPYAQFRDVYTLEQIEQAPMIHYPLTKLQCSPTSDGAGAAVIVSQKFLDERPHLKSQAILIAGQALMTDSPQLYSKSSMDLVGYDMTKRAAQVALKEAGVSSKDIKVAELHDCFSANELILLEGLGFSEPGKAHMMVRNGDITYGGKGPIINPSGGLISKGHPLGATGLAQCAELTWQLRGWANNRLVDGADVALQHNLGLGGAVVINVYKRADGKKSVKMSDEEIKKASQFSYNPAVEARYVTKEEGDKVRSKSVANDYALGDTLEKLQSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIJ6</accession>
    <name>A0A9W8YIJ6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SKY1</name>
        <name evidence="12" type="ORF">N0V83_001001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIJ6"/>
    <dbReference type="OrthoDB" id="2649at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14136">
        <property type="entry name" value="STKc_SRPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000409">
        <property type="entry name" value="CMGC/SRPK protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000076">
        <property type="entry name" value="CMGC/SRPK protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051334">
        <property type="entry name" value="SRPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634:SF9">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="123"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="15"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="278"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="337"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="41"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="278"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="301"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4378168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="627" mass="69233" checksum="BEFCB46A947483F4" modified="2023-11-08" version="1">MNGTDIKKAAINKAKQVAQAANGNGGKKRRKGQDLKPIITTEKQASQDASPASTGSFHYNVQNTASPLAQHKMYGNQLSHSPDSSSSSVDEAENTADEEDSEDYCKGGYHPVQVGEEYKEGKYTIVRKLGWGHFSTVWLSRDNTSGKHVALKVVRSAAHYTETALDEIKLLKKVVDANKDHPGRAHVVSLLDSFNHKGPNGVHVCMVFEVLGENLLGLIKRWNHRGIPMPLVKQITKQVLLGLDYLHRECGIIHTDLKPENVLIEIGDVEQIVKTYVKDEPKQKDSDDSNRNGRRRRRTLITGSQPLPSPLNASFNHAELANFPGSTASLNRIVNENTGKESPTTLPSPSSQDSLVMSGALATSSSPSANLSMAERLGIKSSTNEDDVQKQREKTADLLTKEVSGISLDKSSESSGMSELEKQVENAFETISVKIADLGNACWVGHHFTNDIQTRQYRSPEVILGSKWGASTDVWSMAAMTFELITGDYLFDPQSGTKYGKDDDHIAQIIELLGTFPKALCMSGKWSQEIFNRKGELRNIHRLRHWALPDVLHEKYHFASEESKRIADFLLPMLQLLPVDRANAGGMAGHEFLKDTKGMESRDLGIAVGSKGRGLRGGRRRLRRLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJM8</accession>
    <name>A0A9W8YJM8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_000575</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJM8"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000090">
        <property type="entry name" value="MFS sugar transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF72">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="269"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="369"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="407"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="435"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="20"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="488"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="509"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4377745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="524" mass="58575" checksum="47706D960B2AE219" modified="2023-11-08" version="1">MFKAPHFDWYRRGFKLRAAITIACQLAFVLFGYDQGVFSGIVGNEDFLNTFDHPSPGLEGIIVSIYDLGCFTGCILAFIFCEKTGRRLAMWIAMVWIIVGGALQASAYSVPHLMIARFLTGIGTGIETSTVPMYQSELCEASKRGRLVASEPLFVGFGIEVAYWFDYGMSFSSGSIAWRLPIACQIIFAIIVIILVFGLPESPRYLYKHGRNEEAMQILCDVYDGTPDDPKIAKENQEVLEALKIEQEYGEYQWSQLLKKDKVQTGRRVLLAYGAQFMNQMGGINLVVYYITSVLQLNVGLTRDLSLLLGGVINLMFFLGSLLPTFTLDRTGRRKPMMWGSFGCGASMMMIAILLSFQPQGGSVAKATASGSVAFFFTYMLAFGMTMNCIPWVYVPEILPLHVRAKGTAVGISANWIWNFFVVMITPILLNSLAWKGYLIFMILNFSFIPLVFFCYPETSNLTLEEVDWLFYEGNVVKRSRRVAKQGWEQAEGTHPAGILETGSMSSGKKAESNVEKREEHTTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YKF4</accession>
    <name>A0A9W8YKF4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl13</name>
        <name evidence="5" type="ORF">N0V83_000968</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YKF4"/>
    <dbReference type="OrthoDB" id="10264538at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000003">
        <property type="entry name" value="60S ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00499">
        <property type="entry name" value="Ribosomal_eL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001380">
        <property type="entry name" value="Ribosomal_eL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018256">
        <property type="entry name" value="Ribosomal_eL13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01294">
        <property type="entry name" value="Ribosomal_L13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01104">
        <property type="entry name" value="RIBOSOMAL_L13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4378135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="215" mass="24680" checksum="299350694723E7DC" modified="2023-11-08" version="1">MAIKHNQQIQKNHFHKDWQRYVRVHFDQPGKKKSRRNARVAKAAKVAPRPVDLLRPVVRCPTIKYNRRVRPGRGFSLVELKAAGIPRKFARTIGISVDPRRQNLSEEGLKANVERLQEFRKRLILFPRRNGKTKQGDASAEDIKVAKDAEKIVSSTASVLPIKNVIEFEQGPISDYKGEENAFRKLRDQRSEARLVGVREKRAKAKAEEADSKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YKU9</accession>
    <name>A0A9W8YKU9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YKU9"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4378137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="338" mass="35958" checksum="A88B5898869EB4FF" modified="2023-11-08" version="1">MEGIGSPVTTRRPSEQIVPTALRVGSPAVGATPPPQARPPLARKNTSGSSNRLSQLFPSRPSSVASISPPSSTPASRRTSYPSPLVPAAEPSYRTPRAPPPPSFAEETSYNPAANPFSQQGATPSLQSSSGTKRLLNRLTSLRNGGSRGGNYNRLDDEESGSSRTRLRGVEEADEAVGYDMSGLDGMPMKKFEPQTKQASTADAMERERHMSEAGFAAEFESLEAGIGAGMASILQKPFTHTPTQPLQGANAGHKRGLSASEIANVQAKDAQKEAEARGDIVAIADIPVDISDFAAGNESDARSIMTTDTGLVKDETQTSYYFPPGMYDAGWQNERRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CGA0</accession>
    <name>A0A9W9CGA0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Clustered mitochondria protein homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Protein TIF31 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CLU1_2</name>
        <name evidence="3" type="synonym">CLU1</name>
        <name evidence="3" type="synonym">TIF31</name>
        <name evidence="6" type="ORF">N0V83_010829</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">mRNA-binding protein involved in proper cytoplasmic distribution of mitochondria.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">May associate with the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CLU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CGA0"/>
    <dbReference type="OrthoDB" id="1414216at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15466">
        <property type="entry name" value="CLU-central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000293">
        <property type="entry name" value="Clustered mitochondria protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2280.10:FF:000002">
        <property type="entry name" value="Clustered mitochondria protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2280.10">
        <property type="entry name" value="Hypothetical protein (hspc210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03013">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033646">
        <property type="entry name" value="CLU-central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025697">
        <property type="entry name" value="CLU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028275">
        <property type="entry name" value="CLU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027523">
        <property type="entry name" value="CLU_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007967">
        <property type="entry name" value="GSKIP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023231">
        <property type="entry name" value="GSKIP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601:SF6">
        <property type="entry name" value="CLUSTERED MITOCHONDRIA PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT EIF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13236">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15044">
        <property type="entry name" value="CLU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12807">
        <property type="entry name" value="eIF3_p135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05303">
        <property type="entry name" value="GSKIP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103107">
        <property type="entry name" value="Hypothetical protein c14orf129, hspc210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51823">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Clu" evidence="5">
        <location>
            <begin position="253"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="545"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="855"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1163"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="545"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="565"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="598"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1171"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1196"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1216"/>
            <end position="1232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4361888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1232" mass="136614" checksum="AAE588FC51A4E22E" modified="2023-11-08" version="1">MPHTFQYSCFHLEHEGQRINDYVELSEVSGLKADSVLTLVEDPYTEKEARLHVIRVRELIGAAGDRTDALHGIMAGLSLHDTVGLDQSGKPKEDGPEQSPLADYDFKSSGAVKDLLPPPQESAPKTIKSIAVSPWNPPPYHLRSKGHLLYLVVMTNENEQHHITAHVSGFYVNKSSNASFDPSPRQAPKAKQAHSLLALLEELSPSFSSSFQQLLEHNAKKELLTIFQLSNAIPANPWLVPAPTSSLTTHQPDLARTQESYLISGVENTETLRDWNEEFQSTREMPKEAVHDRVFRERLTSKLFADYNDAATRGAMLVARGEIAPLNPTEAKDAQIFVYNNIFYSFGADGVGTFGTEGGDEAARVAVGKDVFGVRAVNNLDIQNLFTSGTVVVDYLGKRIVGQSIVPGIFKQRDPGEHQIDYGAVEGKEIVADDKSFVPLFEQLSKALRVKKHPVWDKDSVRHELEGSVETKGLIGTDGRRYALDLYRLTPLDVSWIEAYWSEPSKDGESKSKDKDYPHRMATLRPELVESYGRLKLREYVKNELSKKTNKTETAAKKETKDEESSSEEEDSDDSEEDSDDSEEDSDDEKPKPKPKSKSKEVAKSENKDDAEKKDDAEAPEQDRVDISGFSFALNPDVFSGQNPQSEEDKKEWAQDEAEVRAACDHLSSEVIPRMIQELKDGEVGFPMDGQSLSTLLHKRGVNIRYLGKIAELADKPDPRLQALKRLLIQEMIARGFKHFANSKLRNVSAPFAAPCVAHLLNCLLGAEVSAKPVAEGDSSLKAMYPEADFSYEKLTPETLKKEVLAQIALRYRYDLGQSWIESDKQLQLLREVSLKLGLQLVSKQYAFTKDTLTNGAVPETPAAPQTNGHSTSSKKKKNKTSSPPRDASPAVNLPPQTFHADDVLNIVPVIKEASPKSLLAEEALEAGRMSVAQDQKELGQELLLESLQLHEQIYGVLHPEVARAYHTLSNLLFNLEDKASALELAHKAVIVSERTLGVDHADTVLAYLNLGLFEHASGNTKAALVYVRHALELWKIIYGADHPDSITTLNNAAVMLQAMKQYHESRIWFEASLAICEEVSGKNSINTATLLFQTAQSLALDKDMRGAVNRMRESYNIFKDVLGAEDRNTKEAESWLEQLTQSAVSQAKQLNDLAKGRIRRIQLTGRNPLRPAAATPSPSEAAAAAGGQRSGSSRVDQRKIEDLLKYIEGDSSKTPTKKRTQANPKKRTQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHE7</accession>
    <name>A0A9W9CHE7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosomal protein L22</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RPL22</name>
        <name evidence="4" type="ORF">N0V83_010505</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHE7"/>
    <dbReference type="OrthoDB" id="10259820at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.210:FF:000001">
        <property type="entry name" value="60S ribosomal protein L22 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002671">
        <property type="entry name" value="Ribosomal_eL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038526">
        <property type="entry name" value="Ribosomal_eL22_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10064">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10064:SF31">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL22A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01776">
        <property type="entry name" value="Ribosomal_L22e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4362412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="124" mass="14325" checksum="C72FC662237122C5" modified="2023-11-08" version="1">MAPTTKKGAKAQKVTKKFIINCSQPVNDKIFDIQAFEKFLHDRIKVEGRTGNLGDTIQISQQADGKIEVIAHQEFSGRYLKYLTKKFLKKQQLRDWLRVVSTSKGVYELRFFNVVNDEADEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHP6</accession>
    <name>A0A9W9CHP6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHP6"/>
    <dbReference type="OrthoDB" id="550558at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37844">
        <property type="entry name" value="SER/THR PROTEIN PHOSPHATASE SUPERFAMILY (AFU_ORTHOLOGUE AFUA_1G14840)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37844:SF2">
        <property type="entry name" value="SER_THR PROTEIN PHOSPHATASE SUPERFAMILY (AFU_ORTHOLOGUE AFUA_1G14840)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="213" mass="23576" checksum="4D171B18F75B8F75" modified="2023-11-08" version="1">MEKDPVTKGRLEVLYDTRSEVPGTNIVLLGCTLWSKIPDADVAAVLKKMPEFDEKDGIQDWSIEKHNAEHKRDYKWLLDEINNSQSTTLRPPAGASALPAQASGSATPKSETQLVVVSCFAPELRESLDPWQVDAPWASAYGTNLLDNPPHFSNVKTWISGMTGRTHEFKKGNTTLVSNQRGREGEHVTGLLKDGMSEKQKAGLFDVMRVSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHU0</accession>
    <name>A0A9W9CHU0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Isopropylmalate dehydrogenase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_009048</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHU0"/>
    <dbReference type="OrthoDB" id="10261637at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050501">
        <property type="entry name" value="ICDH/IPMDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43275">
        <property type="entry name" value="D-MALATE DEHYDROGENASE [DECARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43275:SF1">
        <property type="entry name" value="D-MALATE DEHYDROGENASE [DECARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="9">
        <location>
            <begin position="31"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4364454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="410" mass="44547" checksum="20A9CAC95782ABE0" modified="2023-11-08" version="1">MCAREPLKQERNIITKSKMPSHIHSVSRLHRIACIPGDGIGVDITDAAIRVLTTLSSTLGGFEFDFTTFDWSSDNYLSRGYYMPSDGLSQLRAFDAIYFGAVGSPAVPDHISLWSLILPIRKALNQYVNVRPTRMLQGVKSPLGECATEDLDWVIVRENSEGEYSGQGGVSHEDTAHAVATEVSVFTQVGIERIMRFAFETAKGRPRKKLTMVTKSNAQRHGKFPHLIPTEESVLGGFFLVAHGGLGMVLWDKVFYEVAKDYPDVEIDKMLVDAMTVRMVLHPKTLDTIVATNLHADILSDLAAALSGSIGIAPSSNLDPTRANPSMFEPIHGSAPDIAGKGIANPVGAFWSAAEMVRWVGEEKAADGLMKAIENVTARGVKTKDLGGSEDTKGVTDAVCQEIEDLFKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHX0</accession>
    <name>A0A9W9CHX0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009679</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHX0"/>
    <dbReference type="OrthoDB" id="10071171at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01830">
        <property type="entry name" value="XynE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053140">
        <property type="entry name" value="GDSL_Rv0518-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43784:SF3">
        <property type="entry name" value="GDSL FAMILY LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43784">
        <property type="entry name" value="GDSL-LIKE LIPASE/ACYLHYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G00820)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040871430" description="SGNH hydrolase-type esterase domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="SGNH hydrolase-type esterase" evidence="2">
        <location>
            <begin position="230"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="450" mass="48333" checksum="37F75C42877AED27" modified="2023-11-08" version="1">MIFSRLAVLLVGVAVSVQGHAVDGKNVAKRVIRPNPSNGHWIDAWASMPQLTEPANLPPAPFNQTGAVFVNSTIRQTLFMTAAASQIRLKISNVFGGSNLPITAVTVALPANNTAGIYQIQPKTLQKVTFSGKDSISIPNGALAVSDPLNFPIKAQQIVTVTMYLATGQTTNSITSHPGSRTTSWWQFGNAVTAESLSITDPSTQSAAHWYFLSSIEAWVPPHQGSLLIVGDSITDGRGSTNNANNRWPDLLFARLQKSSSTKHISIGNLAAGGNRVLADGLGPNAFGRIDRDVLAHPGVRYAMVFEGVNDIGSAPATPAAQTAIYENLIQAYTQIVQLIHAHGIPVFAATITPFSAPSNTTVQPYSDPEREKTRQKVNKFIRESGTFDAVVDFDKLLADKAIPSQLDDEYNSGDYLHPNVAGYQHLADSFPVGIFGEWEDGADEFSWGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI60</accession>
    <name>A0A9W9CI60_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008657</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI60"/>
    <dbReference type="OrthoDB" id="10584919at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4365041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="123" mass="14173" checksum="3FED871860D49A8E" modified="2023-11-08" version="1">MLSRSLSFPLPKDVRNSNPCQHDFLRRIYHIAPSFMTADPDVIPDSQMDAMDEGFLDIDWIGIDDIECTDEQLMDDARPGRRKAKGDQDLKLQSIVKDVKRLSMYEEVPRDSTKENAGDKMKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIF6</accession>
    <name>A0A9W9CIF6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009388</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIF6"/>
    <dbReference type="OrthoDB" id="2148716at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13577">
        <property type="entry name" value="SnoaL_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="15"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="144" mass="15716" checksum="D98B1BFEFECB71D7" modified="2023-11-08" version="1">MHGTPLPSKLAGPPLSDREAVADACFRAFASIDHAEESLLKSSCTADIYTDIASKVCNGYDELKTKVWENVATKLDTVHYLTNMRVSVDTPTTARGYDPESGKFTTGAIYNCDAVKEDDDVWKLKTMKSSHVWGEGDPSIMAGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIG9</accession>
    <name>A0A9W9CIG9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CMP/dCMP-type deaminase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009434</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIG9"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000007">
        <property type="entry name" value="NAD(P)H-dependent D-xylose reductase xyl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="3">
        <location>
            <begin position="312"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4363980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="519" mass="58754" checksum="4D28D46F8A6CAACC" modified="2023-11-08" version="1">MSKDSIKLASGQQMPLVGFGLWKVPKDTAADTVYNAIKSGYRLFDGAYDYQNEKEAGEGIRRAIDEGLVKREEIFITTKLWNNYHSKGHALPMIKRQNDAWGLGYIDLLLIHFPCALEYVDPEKGTRYPGWWMEDKKTVKPARVSIRETWESLEAAVDQGLAKSIGVSNFQAQSLYDMFTYVKHPVSALQIEHHPYLTQPGLIQMAQTHNIAVTAYSSFGPQSFLELPPDFRQRAADVGLLFDAEPVKTVAEKHGKSPAQVLLRWATQRNIAVIPKSNNKDRLAQNLDVTSFDLSKEELDSISALDKGLRFNDPETIEKRIIPLTQKGVASGSKLFGAAILSRSALTPYTIATNNERVSPLLHGEINCIQQFFTADFPDLSTRPDPRRDCIFLATHEPCSLCLSGITWSGFSEFYYLFTYEDSRDLFAIPYDIEILEEVFKVRGKDEAEEQVREKPLYNRNNKFFKARSFADVVSEIEEEGERQKWLAEIERVKSLYNALSETYQGGKKAGTTSSSVWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ05</accession>
    <name>A0A9W9CJ05_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Kelch repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007845</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ05"/>
    <dbReference type="OrthoDB" id="10250130at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019760">
        <property type="term" value="P:glucosinolate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47435">
        <property type="entry name" value="KELCH REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_5G12780)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47435:SF4">
        <property type="entry name" value="KELCH REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_5G12780)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="37355" checksum="4C9B4C70FB9E0B01" modified="2023-11-08" version="1">MPVARPISTLKGTWTRLLDEERLRRSSQVLSVIDQTVCIFGGEVQPRQPIDDKVDVFSLKPGSTNLETKSVSSAPSPRVGSASAVINGNMYLFSGRGGTDMAPIEEDGAVWSFDVARSSWSKIMPADSSKPYPPARSYHCSTSDGENVFFVHAGCPAKGRLSDLWKFDVSTRTWTQDTDAPPPHRGGTSIAYSGGKLYRMNGFDGNTEQGGSIDIFDVASNTWSTKTFNADGNDGPEARSVCVLLPVHLARKDKLITLFGERDPSSLGHAGAGKMLSDVWVYDISEDWWTQLDHDGRDGKPDPRGWFDADVIKSQSGNDSVVIHGGLGENNERLTDVWHLTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ27</accession>
    <name>A0A9W9CJ27_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008644</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ27"/>
    <dbReference type="OrthoDB" id="4161095at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="141" mass="15968" checksum="C565F13C758391AF" modified="2023-11-08" version="1">MYNTYVHYLTAYNHITNLSPTLYNTKPKPPVPPATMSNNSSSTFQSFSTSFSSSSHNGQTSSRSETTYSDPSGTHVHRTSQEPGQEPREERFETDHAGRRVEGASQRRIEDVTDKDEGQEQRDKEYEERIEGEYAKREGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK29</accession>
    <name>A0A9W9CK29_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein artemis</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">DNA cross-link repair 1C protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">N0V83_008057</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK29"/>
    <dbReference type="OrthoDB" id="5561659at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011084">
        <property type="entry name" value="DRMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF8">
        <property type="entry name" value="PROTEIN ARTEMIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07522">
        <property type="entry name" value="DRMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="15">
        <location>
            <begin position="28"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="DNA repair metallo-beta-lactamase" evidence="14">
        <location>
            <begin position="440"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="515"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="515"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4366421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="696" mass="77591" checksum="55479D40A29E8F03" modified="2023-11-08" version="1">MSTFKGIIAEFPQIRIDYFRQQPGHKAPLACFLSHVHSDHLAGLEALRAPFVYCSAATREILLRLEKYHYRINFAKGLLESRNVTYDRSMRKLAKPLPLDTPTVIELSPGNSIRVTLIDANHCVGAVMFLIEGEGKAILYTGDIRAETWWVNSLVQNPVLLPYTLGVRRLDCLYLDTTFATKSKPYREFPSKAEGIRELLAKVDKYPQDTIFYFHSWTFGYENVWIALSTYLDSRIHLDDYRARIYGSLSTLEGKQLREIGLSVPADNKSLRELGLEIREAPALCGFRNGNHIQPGCLTSRESVRIHSCERGMGCRVMDRDTDAKVVHIIPIITRANGADIFELGAGGGKGDLDQKEELETGDTTDAAKLLELCAGFIDDEELLSKVSVLLHQTPNGKLDLAAMPAQKEVRASQNAFSIQTLASILASNVSKDEATEPPRNRTIQFPYSRHSSYMELCGLVEAFRPMNVYPCTVDEDGWTPAVSMRELFGSFCSRHVFRHDAEMMDMYNLKVAHEGKQNQHQAETQRDTQASDRALDVSPIRVKRVRSEDSEDLKSPDYVTPPTTVYGAKENITQTRRVRISRASPTLTATNLEPFNAASNRNTPAASIMNNPDLLAPTLTATGSLPTLPQSTVELSTPGAMITMRGSNGTKTKRQKITNRVIAYQAALGVHGLTWSDYGGLISTRSKADQEELEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKK4</accession>
    <name>A0A9W9CKK4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Adenosylhomocysteinase</fullName>
            <ecNumber evidence="7 11">3.13.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SAH1</name>
        <name evidence="14" type="ORF">N0V83_006930</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 11">
            <text>S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine</text>
            <dbReference type="Rhea" id="RHEA:21708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.13.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="10 11">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.13.2.1" evidence="7 11"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKK4"/>
    <dbReference type="OrthoDB" id="10007170at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000004">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000004">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00563">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004005">
        <property type="entry name" value="PRK05476.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF0">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10 11" id="KW-0520">NAD</keyword>
    <keyword evidence="4 11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-adenosyl-L-homocysteine hydrolase NAD binding" evidence="13">
        <location>
            <begin position="194"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="160"/>
            <end position="162"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="225"/>
            <end position="230"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="302"/>
            <end position="304"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4367349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="449" mass="48798" checksum="9496ABD40889BAE5" modified="2023-11-08" version="1">MSAPANKFKVADISLAAFGRREIELAENEMPGLMETRKKYAEDQPLKGARIAGCLHMTIQTAVLIETLKSLGAELTWTSCNIFSTQDHAAAAIAAAGVPVFAWKGETEEEYEWCLEQQLTAFKDGKSLNLILDDGGDLTALVHKKYPEMLKDCYGVSEETTTGVHHLYRMLKGKGLLVPAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKVAVVAGFGDVGKGCAQALHSMGARVLVTEIDPINALQAAVSGYQVTTMEKAASQGQIFVTTTGCRDILTGAHFEAMPNDAIVCNIGHFDIEIDVAWLKKNAKSVTNIKPQVDRFLMPSGRHIILLAEGRLVNLGCATGHSSFVMSCSFTNQVLAQIMLYKASDEAFGNKYVEFGKTGKLDVGVYVLPKILDEQVALLHLAHVNVELSKLTEVQAEYLGLPAEGPFKADIYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKU7</accession>
    <name>A0A9W9CKU7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007234</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKU7"/>
    <dbReference type="OrthoDB" id="10266249at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="168" mass="18446" checksum="4F1F7281454CCB89" modified="2023-11-08" version="1">MYEKDGLMIAWRDTSVAAVKRRNEIAEQEAAEARSGAGEDKMLKRMAKRAQEEAEAKKAIEAKRAAAAAAMEKKDTTPPAASASANKDNVPTEEGKIDSPVDEMKKEDEAKKDSEEPKVEAAPAPIKLGFSLKSKTLPQNKASPGHRNVFKRKKDDALEQKPVKKFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKZ0</accession>
    <name>A0A9W9CKZ0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Choline transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">HNM1</name>
        <name evidence="8" type="ORF">N0V83_006157</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKZ0"/>
    <dbReference type="OrthoDB" id="2417308at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF7">
        <property type="entry name" value="CHOLINE TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="313"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4369074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="337" mass="36526" checksum="2FC5C946A37CE9D8" modified="2023-11-08" version="1">MESSAEKQLSPSVSPDVETAQNSPVDPDALKLAEMGYTQDMSRNFSVWSVLGVGFSLTNSWFGISAALITGINSGGPLLIIYGIMLIALISTVIKTWHVFLAYQIANIFCFFFNCYGKTLPTVAQVTLWTSLVSFAIILITVPAVAPTHQHAKFVFATFINNTGWEQGGIAFIVGLVNTNWSFACLDCATHLAEEVHRPEKMVPIAIMGTVAIGFVTSWFFSMAMFFSIVGDFADIGASATGVPILEVFNQALSHKAGAIVLEALIIATGLGCLIASHTWQSRLCWSFARDRGLPAHRWLSKVDKRLDVPLNAHFVSCVIVAIMGCLYLASLTAFNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL80</accession>
    <name>A0A9W9CL80_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">26S proteasome regulatory subunit RPN1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RPN1</name>
        <name evidence="9" type="ORF">N0V83_006428</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the proteasome subunit S2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL80"/>
    <dbReference type="OrthoDB" id="10252509at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000026">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016643">
        <property type="entry name" value="26S_Psome_Rpn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002015">
        <property type="entry name" value="Proteasome/cyclosome_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041433">
        <property type="entry name" value="RPN1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040892">
        <property type="entry name" value="RPN1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01851">
        <property type="entry name" value="PC_rep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18051">
        <property type="entry name" value="RPN1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17781">
        <property type="entry name" value="RPN1_RPN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015965">
        <property type="entry name" value="26S_Psome_Rpn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 9" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RPN1 N-terminal" evidence="7">
        <location>
            <begin position="59"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="26S proteasome non-ATPase regulatory subunit RPN1 C-terminal" evidence="8">
        <location>
            <begin position="843"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00057191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR015965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4368073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="898" mass="99785" checksum="8429517E0123BB0B" modified="2023-11-08" version="1">MAKEDEKKPTAAEKGKGKAVNGDAGKEKDTKVDGEDKKGAAATTGEELSEEDQQLKSELDMLAERILESDTSLYKPALEQIKDFIKTSTSSMTAVPKPLKFLRPHYENFEKAYASWPEGEDKQSFADMLSVLGMTYSDEERLDCLKYRLQAPSTDLGSWGHEYMRHLALEIGREFQNRLNDDKSTDDVTDLASNLVPFFLKHNAEADAVDILSELEMIDQIEQYIDENTYPRVCLYMVGIVPLLTYPDNDKFLRTTFQIYRKYNQYTQAVALAIRLNDRDLIEDVFNSTKDKAIRRQMAFLIARQRIWFDVSDDDDSELQECLNNTRLPDHFKALGKELNILDPKTPDDIYKTHLESSRTAGLTNTDSARHNLASAFVNAFVNTGYGNDKLMLNSDSKSSWVWKTKEEGMLSTTASLGMLLLWDVETGLDKIDPYTNVDEDMVKAGAMLATGILNSGVRMDSDPALALLGDEDNLAHKNRNIRLASIIGLGLAYAGSNKEELLDLLLPIVSDTSLDMQLSAMAALSLGLIFVGSANGDVTDALMNTLLDEDRTKQLKDKWTRFMALGLALLFFGQQEEVDVILETLKALDHPMAKSASVLTEVCAWAGTGAVLKIQQLLHICNEHIEDDSEEKQGDELLQSYAVIGLSLVAMGEDVGQEMILRTLGHLMHYGEANIRKAVPLALGLISPSNPQMKVYDTLSRYSHDNDNDVAINAIFAMGLCGAGTNNARLAQLLRQLASYYHRDPNALFMVRIAQGLLHAGKGTLSLNPFHTDRQVLSRVASAGLLTVLVSLIDAKSFIVGDSHYLLYFMATAMSPRFLITLDEELKPLTVNVRVGQAVDIVGQAGRPKTITGWQTQSTPVLLAHGERAELEDEKYISLTNVLEGVVILKKNPEWED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLF6</accession>
    <name>A0A9W9CLF6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005613</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLF6"/>
    <dbReference type="OrthoDB" id="2391627at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019182">
        <property type="entry name" value="Cytochrome_b-c1_su10_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28254">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28254:SF1">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09796">
        <property type="entry name" value="QCR10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="101" mass="10962" checksum="5CA7193CFBA7BAFB" modified="2023-11-08" version="1">MSAASPPHHGKTPAAFSKRTPYKAYKSPFGPQYTIAKHYHGITARTLVKAGVLAGGFGGAAGFFALFFFAEVPKVRDDIMKKIPVLGDYFNKEVAPEDNPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLH4</accession>
    <name>A0A9W9CLH4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_005673</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLH4"/>
    <dbReference type="OrthoDB" id="5379086at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4369909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="481" mass="53271" checksum="E3B14920D783EACE" modified="2023-11-08" version="1">MAATSGTSGSGEPDMVLIGELLEEISRKPPAVGARKLLVEHYLSVGWLDAAMDNAKELKSLAPKDPDVPKFLQLLEKKPQPPATEKESVVVATAPKVETREWDPKTGRYKKSTTKLSKTPEAPSVEIGDLEHAREDLTQGHWALRARAKSVLVDLLQLQALRKQAGLSQSKSKNLSKIEAIVYPRTVPTSSKTGPPGSARSVARIIRDNPKEATSLIITDLEDTMSWTRAPHGKPLRINNDALRDTLVKRKAALESHLTDKLKVHCELALMHIEHEHEDIDRNYANTETMLGDEVKDIPRDNFYVTEDNYAWDMDELVQAIRANSGVLRNPLSKQMFTPKDIKGILIHPMGKPLAALAVEQHEMSKGVRLATIEQMEKLSNILLEDQSADTLPSRKAVDEFLAYIATLPDLEQKAIDGLKCPAKDSHTGQAYDFSIGESVRDAKGNRVCFHKTGDFIKQAAEHLRQNRGVVATDSDKCVVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLH9</accession>
    <name>A0A9W9CLH9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-galactosidase</fullName>
            <ecNumber evidence="3">3.2.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_005947</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyl hydrolase 35 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLH9"/>
    <dbReference type="OrthoDB" id="1657402at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.102.20.10:FF:000001">
        <property type="entry name" value="Beta-galactosidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.20.10">
        <property type="entry name" value="Beta-galactosidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.390.10">
        <property type="entry name" value="Beta-galactosidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018954">
        <property type="entry name" value="Betagal_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037110">
        <property type="entry name" value="Betagal_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025972">
        <property type="entry name" value="BetaGal_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036833">
        <property type="entry name" value="BetaGal_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025300">
        <property type="entry name" value="BetaGal_jelly_roll_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031330">
        <property type="entry name" value="Gly_Hdrlase_35_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001944">
        <property type="entry name" value="Glycoside_Hdrlase_35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23421">
        <property type="entry name" value="BETA-GALACTOSIDASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13364">
        <property type="entry name" value="BetaGal_ABD2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10435">
        <property type="entry name" value="BetaGal_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13363">
        <property type="entry name" value="BetaGal_dom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01301">
        <property type="entry name" value="Glyco_hydro_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00742">
        <property type="entry name" value="GLHYDRLASE35"/>
    </dbReference>
    <dbReference type="SMART" id="SM01029">
        <property type="entry name" value="BetaGal_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117100">
        <property type="entry name" value="Beta-galactosidase LacA, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040899046" description="beta-galactosidase" evidence="9">
        <location>
            <begin position="26"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-galactosidase" evidence="10">
        <location>
            <begin position="374"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4368865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="975" mass="106008" checksum="567320E77123FDE4" modified="2023-11-08" version="1">MWALRGLSWLSLLSTLTILSGGGVAAQNNTTPSQWPVHDNGLNDVVQWDHYSFKINGKRLFVFSGELHYWRVPVPEVWEDLLEKIKAAGFTAFAFYGNWAYHSANNGTLDFTTGSHDFTKLFEIAHRVGLYVITRPGPYVNAEANAGGFPLWLTTGAYGALRNDDPRYLQALDPYFSKFSELTSEHLVTKGGNAFVYQIENEYGEQWNDTTKKIPNDAAGRYMAALEDSARANGIDIPLIHNDPNMNTKSWSKDFAPGAVIDYYDHFKEVSPTQPSFFPEFQGGSYNPWGGPEGGCPGDIGADFANLFYRNLISQRVTAVSLYMVFGGTNWGALAAPVTATSYDYSSPISENRKIGSKYYETKNLAMFTRVAEDLTVTDRIGNSSSYTTNSAVEASELRNPSTKAAFYITIHSYSPSGTKESFKLHVSTSIGNLTIPRHAGSIVLDGHQSKILVTDFAIGNHTLTYSTGEVLTYALIDGKPVVVLSNGVGESVEFHVKGAKTGSLISNHAGLNATFYSEAHGVTTNIAQISGLSVYQFDNGVKVIVTDKPTAYLFWAPNLTNDPLAPVDQSVLVQGPYLVRSVSKEDGVLAIKGDIANTTAIEVFAAECEETLSWNGKVLQTKRTPYGSLKASIVASNSTIQLPALDNWKVSDGLPEKLRNYNDSGAAWVTADHTTTPNPTEPDTLPVLYVDEYGFHNSFHLFRGYFEGSATGVKLAVQGGLAFGWSAWLNGDLVGSFLGNTSLETGNHTLSFANATLFENSTNVLLVAQDNTGHDLRADAVKPRGILGATLQGADFTSWKIAGEAGGESVQLDPVRGPLSEGGLTAERLGWHLPGFDDSAWNSSSPSTGFEGAGIYFYRTTIPLNIPKAVDASLAFVLDASGSQAVRVQLFVNGYQYARFNPFVGNEKKFPVPPGILNYAGDNVIGLSVWAQSEEGAKVDVQLVTEYAVESSWSSRFDSEYLRPGWTQDRLAYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLU8</accession>
    <name>A0A9W9CLU8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ER lumen protein-retaining receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ERD2</name>
        <name evidence="12" type="ORF">N0V83_005642</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ERD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLU8"/>
    <dbReference type="OrthoDB" id="7694678at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046923">
        <property type="term" value="F:ER retention sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000133">
        <property type="entry name" value="ER_ret_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10585">
        <property type="entry name" value="ER LUMEN PROTEIN RETAINING RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00810">
        <property type="entry name" value="ER_lumen_recept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00660">
        <property type="entry name" value="ERLUMENR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00951">
        <property type="entry name" value="ER_LUMEN_RECEPTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00952">
        <property type="entry name" value="ER_LUMEN_RECEPTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4369878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="202" mass="23683" checksum="5E773DE2285BA646" modified="2023-11-08" version="1">MVFAIPDNSSKQSASGISFKSQFLYLVVYLTRYVDLLWTFYEPKSLYNTVFKIVFISTSAYTVYLMVNDYKPTHDPNLDTFKVQYLLGASAVLAILFPYKYSFSEILWAFSIWLESVAILPQLFMLQRTGEAETITTHYLFALGAYRALYIPNWIYRYFFEVPKFYDPIAVIAGIIQTILYTDFFYIYWTKVIQGKKFNLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMH5</accession>
    <name>A0A9W9CMH5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DNA polymerase delta small subunit Cdc1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cdc1</name>
        <name evidence="8" type="ORF">N0V83_004912</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase delta/II small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMH5"/>
    <dbReference type="OrthoDB" id="3763at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07387">
        <property type="entry name" value="MPP_PolD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.50:FF:000006">
        <property type="entry name" value="DNA polymerase delta subunit 2, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040663">
        <property type="entry name" value="DNA_pol_D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024826">
        <property type="entry name" value="DNA_pol_delta/II_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041863">
        <property type="entry name" value="PolD2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416:SF0">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18018">
        <property type="entry name" value="DNA_pol_D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase delta subunit OB-fold" evidence="7">
        <location>
            <begin position="47"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="6">
        <location>
            <begin position="219"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4370394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="498" mass="55038" checksum="0C6F6E5BAAAFEA78" modified="2023-11-08" version="1">MDAPDGDMLKGAPFGDAPYIPSRNPSAYSPLHTFELPKGSDKHYGQQFADMYFLRLAMLKKAVKQKAEDAWSDFKLGDEKASFVERVLDVRQGELCWIVGTVYMEMDLKPNVLDDISKEHWIAAPPDRETYASSSGDDQMMLEDESGRLRVKGESLSSHYVTGCVLAALGTEEADGTFQVIATQYADLPRQPQRWEREDSALSRAKKPTPKHERAGRLAIVSGLDITGADDDDISLDLLLEYLTGEAAGPPTQTEASMITRLIIAGDSLAQASPILSREEFASKKTKTRNYGYDASSYNASPAERLDTFLSELLPSLPVTLLPGASDPANVALPQQPLHPALFPESRLFAEPPATSNETIYGLDTVTNPWDGDIEGYRVLGTGGQTVADLRKYVKKVATLEVMEMMLRWRCIAPTAPDTLWCYPFQDDDPLLLKDCPHIYFAGCQERFGKRVIEGPAGQKVLLVSVPRFRQTGTVVLIDMESLEVELVNLKIRPRASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMX9</accession>
    <name>A0A9W9CMX9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMX9"/>
    <dbReference type="OrthoDB" id="21474at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="378"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="481" mass="54918" checksum="B61CD4A9A5759AE8" modified="2023-11-08" version="1">MARPKACISEDQKYQGKLYKEKKPKGQHQKKDTAQQDNSQALTPHNAYVEDAPDGDNAVALVDAPPRAPTPPPAAHSLGYHEQAALPPVNVFDFLDGSQTPSAPKAHFSIDESRMLEDSQPPVYEEQYVYTEEQHMPSITDVMAFQVQDDDTSFAQNGFTYGEEPIRPSKERYDSYATPAPKSKHSRTKSRDTDVETTSKKTDRKRKRNSPTELDMSLVRAQEERDIMMSDAPPMLHSGLTGGLNRLLARPEFPPSPEDSGEYANSPLSPMKRAKQGNSKALMRAQREYEIQEQKQKKAAIKEERAREEEEKKEKERGRDRDRKERKSSTALVKIRHKKKRDESTKDIRRIRRRQYSSSVSPAPRERKSIKAIEYHRSGSGSPAPSGNGQLIVRPNGDIAPIETETDARAELFMSFITKGPESERGMSVNKALKRYHRERYDRWDREPSKAEEEKELWKNLRLKRNDRGEIVLFFAPPESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN20</accession>
    <name>A0A9W9CN20_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sodium/calcium exchanger membrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_005307</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN20"/>
    <dbReference type="OrthoDB" id="407410at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051359">
        <property type="entry name" value="CaCA_antiporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12266:SF0">
        <property type="entry name" value="MITOCHONDRIAL SODIUM_CALCIUM EXCHANGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12266">
        <property type="entry name" value="NA+/CA2+ K+ INDEPENDENT EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040889138" description="Sodium/calcium exchanger membrane region domain-containing protein" evidence="9">
        <location>
            <begin position="18"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="513"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="546"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="575"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="604"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="636"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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        <location>
            <begin position="1598"/>
            <end position="1607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1607" mass="167729" checksum="AD0AFDB626D5EAB6" modified="2023-11-08" version="1">MEKIKHALSGNKKSESEDATQDTHTSSSTVAGEQHPIYDQFTGKKGAGKEGTLTAAGGPYAQVQTTSGEPYVALAQPGSSVGHGHGHGKEEHLGTDGTIGAASTTSGAPIGSSSNTTGGPLSSSNNAREPIGGSTTSREPIGSHGTTSGGHLRPDASPTGKEGLNTGISDASIKSGVIGFGAGERQEHAALPTHNPTSEYMDRNQVVGGGDPGTAGMTEGQNLKSGAGAQPASGLEQTTGQQPYENTLRQQSYTADTDRSFPLAGGVASRQPHDNTPLTQHRAVGEQEPLTGENDPNVHKGHGKEELAGAAAAGTAGGLYAHHHNKEKDEHATPTQHKTTTEQEPDHKEKKSGILGGIFSRDHKDKDEHASPAQHKTINEREPGSKEREVGVHDDHDREKLAGAAAAGTAAGGGLYAHHHNNEKDANVSPTQHKTTTEEDPGAKEKKTGILGGIFSRGHKDKDEHTSPTQHDTINEREPRTTEQGVGVHDNHDREKLAGAAAAGTAGGLYARHQHEQNSPTQHNTISEREPGTKEREVGVPEGRGQEGLPGVAAGGITSREPHAYTTPTQHQTISEREPGTKEREVGAREGHGREGLAGAAALATAVGASKTLPRSEERDVRDQGLETRQATYGDVPPATYQSSNTTDRLPGSTTATPFDNTRTGPTGSATHHHNPEALAAATAAASKSQEPGYGTQDRSFPGASQPTSGGPTSGSFGAGALSDERPQGTRSASYRHVPGGFPTPTPGDESRTFLDYHPVIAPTSGSDVPSGQSSALGPSSTDAPTSQHELRHTGSLEQPQSRSSDLPGEHHQDEHHYGRDAALAGGLGAGAAGLGYAASQKQHEPSDAGSKPLYGESSPYSAKPLDPRALGTKANLEEQRYDPQARSETLPHQAAQTSNPSGLSAASGPAASHGTAKTEDPQHHYGRDAALVGAGAATAGGLHHELQRNDTPGTGTAVLPQDSSYASQPLSSSTAPQQSSATSAGSFPQQRSTGNDTFYGAAGAPAPVVGQSPQQQQPLSNLGHTSTSGPTATPEKDSQHHYGRDAALAGAGAATAGGLYAANRDDKNETGPASKTIGPHDSNIANILDPRVKPDPSKQKPNTTAGPHQADTLNRADPRVSEKDGQQGEHHYGRDAAVVGGTGAAGYGAHEAAKAYDEHRSTQPGAAMGEQRYDTTAPGATRSNPVPTQSQYNYNDPSTASHANRNAALGTGAGLGAAGAGTAAYADSKHGDNTQNLPLHQKQDFASSGAPPSYQNQVPAETQTEKHHDKRDAALLAGGAAAAGGGAYAYSQHEEAERERARLEKEQQEHLKKEAHDREKEQHRLEKEQHTHEKEAAKHEKAQHKHEKEIAALEAKHEKDIAAQEKEQHRLEKEQYKQEKEADKEARKQEKEQEGEEKKKGGLLGFLHRDKSKKEKNTSSPSPESSPRHSKEYAAGALGAGTTAAAYDEEQDPNSPRWKGKNRLHKDPPPGHPAREALEHQHEGGLAGKREHIGVDGPIGDANLISTDRGVHDIHDTTTNQGYTGTGTGVGGHHDPATSTTTGPLAGTAGHYETGGQGYENIAAVEPTVEGFGNQNAPGATTGYAGHHTVGQNADTEGIRKGDTLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPG8</accession>
    <name>A0A9W9CPG8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Alpha-1,6-mannosyltransferase Och1</fullName>
            <ecNumber evidence="13">2.4.1.232</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">och1</name>
        <name evidence="13" type="ORF">N0V83_001811</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.232" evidence="13"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPG8"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033164">
        <property type="term" value="F:initiation-specific glycolipid 1,6-alpha-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.20:FF:000002">
        <property type="entry name" value="Initiation-specific alpha-1,6-mannosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039367">
        <property type="entry name" value="Och1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834:SF1">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="67"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="96"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4374735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="495" mass="56404" checksum="9A491EEE23A9DDC5" modified="2023-11-08" version="1">MQEAAAQKTRIVSAAAFPFRQQAGALSFLSSYRRKRPATMLTFKKALVLSLACLTLFFIFKSQHSSSTAPTFRNAHDLPRPTAWENPKTPKASQEPLKESTKQTEKTDKAFNTPDVQPKKPKLQTQLSADELRNKPLKEQLEYQFPYDVDSKTPAYIWQTWKYTPAQGEFQEIFREPEASWSIHHPNFVHEVITDNVAVHLIRHLYASVPEVLEAYNSLPLPVLKADFFRYLILLARGGIYSDIDTTALKSAADWIPHDVPANSYGMVIGIEADPDRPDWHEWYSRRIQFCQWTIQSKPGHPVLVEVVANITRETLNRKKAGTLNKDHKLIIEFTGPAVWTDSIFSFFNNPEYFDMSTSRGNITWENFTGMKTPKKVGDVIVLPITSFSPGVKTMGAGEEDDPMAFVKHNFEGMQIFLFLRQSFSFESMIASVVLRCTCTTFRGPDGRIDAPNRLPTLHPLRVPDFSVLTSNRHLEARKRAPYWPDFFINSAAIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPH9</accession>
    <name>A0A9W9CPH9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Coatomer subunit delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RET2</name>
        <name evidence="14" type="ORF">N0V83_003510</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 10">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10 11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10 11">Cytoplasmic vesicle</location>
            <location evidence="10 11">COPI-coated vesicle membrane</location>
            <topology evidence="10 11">Peripheral membrane protein</topology>
            <orientation evidence="10 11">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10 11">Golgi apparatus membrane</location>
            <topology evidence="10 11">Peripheral membrane protein</topology>
            <orientation evidence="10 11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the adaptor complexes medium subunit family. Delta-COP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPH9"/>
    <dbReference type="OrthoDB" id="10266042at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051645">
        <property type="term" value="P:Golgi localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09254">
        <property type="entry name" value="AP_delta-COPI_MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14830">
        <property type="entry name" value="Delta_COP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1170:FF:000031">
        <property type="entry name" value="Coatomer subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000003">
        <property type="entry name" value="Coatomer subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027059">
        <property type="entry name" value="Coatomer_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10121">
        <property type="entry name" value="COATOMER SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10121:SF0">
        <property type="entry name" value="COATOMER SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5 10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="13">
        <location>
            <begin position="288"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="154"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="260"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="179"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="267"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4373217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="526" mass="57983" checksum="D25769469C5DD65C" modified="2023-11-08" version="1">MVVLAASICTRGGKAVLSRQFREMQRSRIEALLASFPKLADSGTQHTICEQDNVRYVYQPLDELYMVLITNLQSNILQDINSLHLFAQVVSSICKSLDEREILKNAFELLTSFDEIVTLGYRENLTMSQIKTFLDMESHEERIQEIIARNKELEASEERKRRAKQLEMQRKEMSRSQRTGGGMGGGMGGGMSGGMGRSPSYPAFTPSVPTTNVADTYDSYEAAKKTTSKPLALGKKGMQLGKKNKTNNMYEQVTGGLPVEEEPLVSPKPAAPAAAAPASARQSTSTDREAVHITTNESISARLDREGLLKSFEVKGEMQLKISDPSFTQVKLDLATGNTRGAQLMTHPKVDKTVFRNDKVIQLADTSKGFPSNMGIGVMKWKLAPRPDDVSDPPITFRVWVEDSGNMFNITVEYELTGGDSLKDVTVTIPYETDEPNVSSFDAVYEVAGDSIEWNIGAVDEANSSGSFEFEAQAGSDAEFFPMRVRFTKSTPFVDVDVSSVTLLSMNQDINFSKDVKSVADVYEIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPR4</accession>
    <name>A0A9W9CPR4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Threonylcarbamoyl-AMP synthase</fullName>
            <ecNumber evidence="3">2.7.7.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">L-threonylcarbamoyladenylate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">N0V83_002225</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Likely catalyzes the conversion of L-threonine, HCO(3)(-)/CO(2) and ATP to give threonylcarbamoyl-AMP (TC-AMP) as the acyladenylate intermediate, with the release of diphosphate. Required for normal translation, by ensuring translation fidelity at the level of codon recognition, appropriate translation initiation selection and maintenance of reading frame. Also involved in telomere replication. Binds to single-stranded telomeric (ssTG) DNA and positively regulates telomere length.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonine + hydrogencarbonate + ATP = L-threonylcarbamoyladenylate + diphosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SUA5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPR4"/>
    <dbReference type="OrthoDB" id="412787at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.870.10:FF:000008">
        <property type="entry name" value="Threonylcarbamoyl-AMP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11030">
        <property type="entry name" value="Threonylcarbamoyl-AMP synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="Sua5-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038385">
        <property type="entry name" value="Sua5/YwlC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005145">
        <property type="entry name" value="Sua5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050156">
        <property type="entry name" value="TC-AMP_synthase_SUA5"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00057">
        <property type="entry name" value="L-threonylcarbamoyladenylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490">
        <property type="entry name" value="SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490:SF16">
        <property type="entry name" value="THREONYLCARBAMOYL-AMP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03481">
        <property type="entry name" value="Sua5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="YrdC-like" evidence="15">
        <location>
            <begin position="113"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="402"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="411"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4375141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="513" mass="55436" checksum="B6C4E5A945CE4578" modified="2023-11-08" version="1">MPTRCPRIPNVKYTRSLARPFPVRSLVQTAASEPRLFPTTTLNVPTPFFTPKTIGLDGSHKTAISQPYRRIVSNMNTDILPVDPQKLGNITFKESKHDLIDQWDIQYAEGPDYESLKRAADELVNTDTPVAFPTETVYGLGADATRSSAVKAIFAAKGRPADNPLIVHVHSLPQLRALLSGRLDKSEEILQAEQDPIPGIYRPLIERFWPGPLTIILPAPESSVLAPEVTAGLPTFGARMPRSILALSLLRLCGRPLAGPSANASTRPSPTAAEHVFDDLQGRIQTIVDGGPCEVGVESTVVDGLSSPPTVLRPGGVTIDQLRQCPGWENVVVGYKDKQEGGASKPRAPGMKYRHYSPKAEVILFEAGAGRPSIEETNAKKAIGIIRTTSWEPALSLDSAAAERNGDSNGTLQHQNTSTTTTTTPSRLSNLLRSVVQHQVPHAHHHESESSDIWDIGLGVGTGDVARGLFSALRELDKKGVDTIFVEGIDDTTGDDIAAAVMNRLRKAAEIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPS4</accession>
    <name>A0A9W9CPS4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein bS18m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_002255</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPS4"/>
    <dbReference type="OrthoDB" id="21463at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.640.10:FF:000013">
        <property type="entry name" value="37S ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.10">
        <property type="entry name" value="Ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001648">
        <property type="entry name" value="Ribosomal_bS18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036870">
        <property type="entry name" value="Ribosomal_bS18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479:SF40">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS18M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01084">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00974">
        <property type="entry name" value="RIBOSOMALS18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46911">
        <property type="entry name" value="Ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4375171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="200" mass="22430" checksum="4D9253D39E5A5226" modified="2023-11-08" version="1">MSIARLPFRAPAAASRACLRCFSSGPQKQDDALLSILGGSSAARTPSTSQQRAGGPSLATRLAREAGLDRPTQSREMVESERRSQYQRQIFRKWQPGDVYAPHDLSGSEQKKWKFGRKRPQEDAFDVLGINPVLEYKNFTIMSEYMTETGRIKHSKDTGLRPKNQRKIAKAIRRAIGLGLMPSVHRHPLVLKKSSPARFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQE2</accession>
    <name>A0A9W9CQE2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_001412</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQE2"/>
    <dbReference type="OrthoDB" id="310217at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF13">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="128"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="141"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="464"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="474"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4376131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="582" mass="64612" checksum="C98123AE7F598783" modified="2023-11-08" version="1">MKPMNVLLRKPWNPLTQRDVSDLFVADFGIASQVQTIGTRLTAQRGTPGYEAPEIRGQGYQAAFSQKSDNPMVLDDIKPSDISPDYSIDLLGLICTMLDSDREQRPNATQVKEKLAALALQLFPPASLHCKSCHQSFPSKSQLDKHLKDRGNPCRYNGQRKPSAIHTPASDDGGLKIRGAAAAPPQHHYEENATEDADPSPCVVCVKHFNSKHRFYAHLHGANHYRGPTSVHKRRAELDLDTEKERAEKREKRLTDWINNQILSRQNTGNADTARIHVSNHEKGLAEHIVPRSLIRTSAVLRAQLNARVICIYFHGSLHVFDLYRAWLNTRNVLTTSELADNNQSEGTTTKEDYICLLCCWGLGHTLQDTLYQDMVASSIIQRVKAWTGTTTVPFVYALTSSMVGIVYGDTAANAPLRRLVVDLAARHGKEADFAHFRQDSAYPAGFIKELMVALGREKERLSAQVQSLRRSQAPPPRASPFPVPSPRRPSTPMERAPTPLFNPDNGRAARQNPPEVSRRNSMPNPSGGAKQVSFQGGRSGPVNDKLEDNEISGNKNSAVLPDECVYHLHTRTGAPCWRAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQX8</accession>
    <name>A0A9W9CQX8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Beta-hexosaminidase</fullName>
            <ecNumber evidence="7">3.2.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">NAG1_2</name>
        <name evidence="11" type="ORF">N0V83_001126</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.</text>
            <dbReference type="EC" id="3.2.1.52"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.52" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQX8"/>
    <dbReference type="OrthoDB" id="428480at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016231">
        <property type="term" value="F:beta-N-acetylglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030203">
        <property type="term" value="P:glycosaminoglycan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06562">
        <property type="entry name" value="GH20_HexA_HexB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000063">
        <property type="entry name" value="Beta-hexosaminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.379.10">
        <property type="entry name" value="Chitobiase/beta-hexosaminidase domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025705">
        <property type="entry name" value="Beta_hexosaminidase_sua/sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015883">
        <property type="entry name" value="Glyco_hydro_20_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029018">
        <property type="entry name" value="Hex-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029019">
        <property type="entry name" value="HEX_eukaryotic_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600">
        <property type="entry name" value="BETA-HEXOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600:SF58">
        <property type="entry name" value="BETA-HEXOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00728">
        <property type="entry name" value="Glyco_hydro_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14845">
        <property type="entry name" value="Glycohydro_20b2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001093">
        <property type="entry name" value="B-hxosamndse_ab_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00738">
        <property type="entry name" value="GLHYDRLASE20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55545">
        <property type="entry name" value="beta-N-acetylhexosaminidase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 7 11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Beta-hexosaminidase eukaryotic type N-terminal" evidence="10">
        <location>
            <begin position="55"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 20 catalytic" evidence="9">
        <location>
            <begin position="186"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001093-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4375848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="583" mass="66170" checksum="A9BC201E108F739F" modified="2023-11-08" version="1">MAILYCSFKTMFPSIFMKQAQVISPEQRGNDSHWRLRKRYASPSFDDYDYYGHNEAKNGISGDDIVEYAVSSAWRTIFKKSLYPWKFHPRDWDEPSPDENTIFLKRVDIKKLDQDPPNLVKPLAGEVDESYRITLTTHGKATIAANTSVGIARGLTTFTQLFYAHSNGEDVYTPLAPVHIYDAPKFQHRGINLDVSRNYFHVKDIKRQIDALAYNKMNRFHLHVTDAQSWPLEIPSIPELSAKGAYRPDLVYTAKDFREIQRHAAIQGVEMITEIDMPGHTSSIHYSHPELVAAFNVQPNWDTYAAEPPSGTLKLNSTAVSDFLETLFNDLLPRVYPYTSYFHTGGDEVNMQAYTLDDTVNSSDKAVLQPLMQKFIDRNHDQVRAMGLTPIVWEEMLLDWDLELGDDVIVQSWQSDEAVAKIVESGHKALVGNYNYWYLDCGKGQWLDFAPSVSSTYWPYNDYCAPFHNWRLIYSYDPLSGVPSSLHHLVLGGEAHLWAEQTDPVNIDRMIWPRAAAVAEVLWSGAKDAAGANRSQIEASSRLSEMRERLVPLGVGAEPIQMPFCLMESEGGLKPQCQLGWTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR56</accession>
    <name>A0A9W9CR56_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N,N-dimethylformamidase beta subunit-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000457</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR56"/>
    <dbReference type="OrthoDB" id="5287072at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046540">
        <property type="entry name" value="DMFA2_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20254">
        <property type="entry name" value="DMFA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N,N-dimethylformamidase beta subunit-like C-terminal" evidence="1">
        <location>
            <begin position="288"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="751" mass="82185" checksum="8350D27BF666D39A" modified="2023-11-08" version="1">MAVTDLEQDADAPEIIGYAEPWIVAPGDSVAIKVSCTEPSYKYRTVRLIQGIDLEHSPKKAEEEVPEIARGEREGKYQTARPGSYAYIRNWEPPLSADGLQVSFYVQPWLLNCPNTQTLVSTLDLGANTGFSIDLNKEGSLDVRVGTERSVEVIRTGLRPQEKAWSKIKLQITGSSIEVRLTHKPFRNETIPPPAAFEGKSGEKASVFTSGSLLIAACQSPATGNSSGALKPHTTDFFNGRIDRLTIKACGQKPSILVKYDFSLDMSSDHIVDVSGRGLNGVLVNAPTRAVKGYDWDGSVADWTQAKFGYGAIHFHDDDLDDAGWETDFTITIPDSARSGAYAVEVEALNGRDTDSITFFVRPTTWTTDNSNKVCFVFSTFTYLAYANERMYDTTRPNGAEMGPSFDITKALKSAEFYKMRRRRDLGLSCYDRHIDGSGVCYSSSKRPILNVRPGYIMWGFSRPREFSADLFMINFLEQEGVPYEVLTDHDLHARGASALDGFNTVITGSHPEYPTYESFNAYNSFAKRGGNLMYLGGNGFYWVSAVDAEGGHPHRLEVRRGDVGVRSFGLPGGEHVLSTNGGRGGLWRSRGMSCNTLFGIGFCGQGTSPGVPFRRTEAATDARFSWMFDGVNGDLLGEYGFGGGASGDEIDRFDIENGSPNDALTLATSTGHDDGYAIAVEDLTYPALETLGTQTNRVRSDIVYYVGAGGGGVFSVGSINWNCSLAWDNYNNNIARLTINVIRGFLEGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRF6</accession>
    <name>A0A9W9CRF6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001642</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRF6"/>
    <dbReference type="OrthoDB" id="3905365at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="67"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="454"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="330"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="144"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="462"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="521"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="547"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="575"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4376359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="684" mass="75093" checksum="A86B1F7664562328" modified="2023-11-08" version="1">MGEAESTTPRSTAEPQLKASKDKNCPFCGQAFTSSSLGRHLDLYIRPKNPKAPDGIHIVDEIRKLRGGITRRQAKGGSISTPKRDDSGTSTPANKKQSVTSEDGSLVQSPEEDDEGLDVGKARAQFKDVSWGRSNRQPSRRGTKTPDLRRDVSRQVQKADRDQRSKTSEQAEIANATELALRELLKSVREANAKASGSGLFDFDPYTLNFPSLCLQVLPAPSTLFSPTPFPTNESWSITPPGQKQFEALNRQVRERLLAYQRQRQINQVYPSGLQSSASSAQNSPLPTPPLFDPDPQKLFCHIADAFNHWMLQTDKTRHEYWQVEILRCYARATDRRRDVEVQLENARREIEHLKANRWTSGAPDVSPVCINLGGDTAKELGRHGIEFRNWDYDRLIDKWRTAIRESRTSTQGMAAQRPLPGTRSCSMTSLAPQTFAAVNEPRHGSPIKVESSMTFAAPPTDNDDAGSDQVDAEGDDDDDEVDLTPHTVSDEDPMNHIQHQAPPQPQHQHQHQGLPLQPTPMHSSQQLPSQMRTPLHVTHAHHAHHAQAQAQAQAQAQAQAQAQAQAWAAARQQMNQSRNQDFRSHQQLSPHVQHIASSANNSRRPSLAMLDPSHAMNPNTVNGVVNSGGMGGAMGGLGGGMEGIEGHAHGHGHGHQDGFLRMDAMGMATGFVGSNDGGVSMGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRP9</accession>
    <name>A0A9W9CRP9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycine cleavage system P protein</fullName>
            <ecNumber evidence="7">1.4.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">GCV2</name>
        <name evidence="10" type="ORF">N0V83_000989</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The glycine cleavage system catalyzes the degradation of glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 7">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GcvP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRP9"/>
    <dbReference type="OrthoDB" id="6537869at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016594">
        <property type="term" value="F:glycine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000005">
        <property type="entry name" value="Glycine dehydrogenase (decarboxylating), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000007">
        <property type="entry name" value="glycine dehydrogenase (Decarboxylating), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000007">
        <property type="entry name" value="Glycine dehydrogenase (decarboxylating), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003437">
        <property type="entry name" value="GcvP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049316">
        <property type="entry name" value="GDC-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049315">
        <property type="entry name" value="GDC-P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00461">
        <property type="entry name" value="gcvP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773:SF1">
        <property type="entry name" value="GLYCINE DEHYDROGENASE (DECARBOXYLATING), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773">
        <property type="entry name" value="GLYCINE DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21478">
        <property type="entry name" value="GcvP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="8">
        <location>
            <begin position="91"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="8">
        <location>
            <begin position="584"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="884"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR603437-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4378156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1077" mass="117025" checksum="3669046FF9A4A39C" modified="2023-11-08" version="1">MRTACLRAAGSASRLARSPCVAHLSRPQLAGSAPRAIAYQSQQYLQKRSFQQNASTSQQAAAVAAADDVFDTPPQQSGSAKPIEALDTFPRRHIGPSAESAEAMLKALDPPVASLDEFVKQVIPADILSSRELKIGKLSKHHKGKWQSDGVPESVFLKNAKRIMDQNKPGKSLIGQGYYNTKVPEVIKRNVLENPAWYTSYTPYQPEISQGRLESLLNFQTMVSDLTGLSIANASLLDEPTAAAEAMTLSMGMMPLSKQKSKNKTFLVSERCHPQTIQVLYSRAEGFGINIEVADVLKDNSKRVEELGNDLVGVLAQYPDTEGAVSDFRGLADKVHKTGALFSVATDLLALTLLTPPGEFGADIAFGNAQRFGVPLGFGGPHAAFFACDEKYKRKIPGRLIGLSKDRLGNPAARLALQTREQHIRREKATSNVCTAQALLANMSAMYAVYHGPKGLTAIAKQVVAKARLVQQALIENGFKTGQRGKMDNAPALFDTFVVETGDKTEGIIADLERRGLLVRRLDDKHIGISVDETTTSQTISNLLQPFRELGKGDKTAFSEPIDIEAPAPFKRTSEFLTHPVFNSHHSETEILRYIHHLQSKDLSLVHSMIPLGSCTMKLNATSEMAPVSFDTVGNLHPFLPLERAKGYIEMIKQLEDDLADITGFHAVSLQPNSGAQGEFTGLRVIRKYLEQQPGKKRDICLIPVSAHGTNPASAAMCGMRVVPIKCDTATGNLDMADLKAKCEKHSEELGAFMVTYPSTFGVFEPNVKAACDLIHQHGGQVYMDGANMNAQIGLCSPGEIGADVCHLNLHKTFCIPHGGGGPGVGPIGVKSHLTPFLPGHLRGETGGAQAIQPVSGAPWGSASILPISWAYIKMMGSVGLTQATKITLLNANYILSRLKPHYPILYTNDQGRCAHEFILDVRGFKATAGVEAIDIAKRLQDYGFHAPTMSWPVANTLMIEPTESESKAELDRFCDALISIRQEIREIEEGEMPRGKNVLNLSPHSLLDLIGGAGEWDRAYSREKAAYPLAYLREKKFWPSVTRLDDTYGDTNLFCTCAPVEPDDSDITGAAAPTPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRS6</accession>
    <name>A0A9W9CRS6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dephospho-CoA kinase cab5</fullName>
            <ecNumber evidence="4">2.7.1.24</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CAB5</name>
        <name evidence="4" type="ORF">N0V83_000218</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.24" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRS6"/>
    <dbReference type="OrthoDB" id="247245at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02022">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001227">
        <property type="entry name" value="Dephospho-CoA kinase CAB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00376">
        <property type="entry name" value="Dephospho_CoA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001977">
        <property type="entry name" value="Depp_CoAkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00152">
        <property type="entry name" value="dephospho-CoA kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695:SF46">
        <property type="entry name" value="BIFUNCTIONAL COENZYME A SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695">
        <property type="entry name" value="DEPHOSPHO-COA KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01121">
        <property type="entry name" value="CoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51219">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4377393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="266" mass="30282" checksum="1DFACC4680AF9201" modified="2023-11-08" version="1">MLLLGLTGSIATGKSTVSSLLSKPPYSLPIIDADLIARQVVEPGTAGYNAIVKYFSPTTPDLLLPDATPKGRPLNRPALGRRIFGSGEEKERDRKMLNSIVHPAVRKEMYRQMIWAYLKGNWAVVLDVPLLFESGWERYCGTILVVGVTDPSIQIARLRNRDAHLTEEDARNRVMSQGDVREKAERCLRRGPGRGVVVWNDHDRGYLEKEVQRVMQDVRGRSPRWWTFLCWVCPPLGALAGLASWYRMKKVQEEWENEKKREKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYP1</accession>
    <name>A0A9W8XYP1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_010484</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYP1"/>
    <dbReference type="OrthoDB" id="3772943at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF39">
        <property type="entry name" value="FAD-BINDING PCMH-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="6">
        <location>
            <begin position="33"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4362391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="390" mass="43307" checksum="4112EFB3A0DA5122" modified="2023-11-08" version="1">MGFGFLGNVFKPGTADYEKANNIYATSTYGVERDMNPGLIYQPVDIPDIQEVLTIANRSDPFTPVAIRTGGHQYSGASSTNHEGIQLDLQSTFRDPVNDLQMIRKDGKVFIRSSVSWKLQELYNFLLKNGVFLPTGQCITVCLGGHVQTGGYGMLGRSFGLLGDYITELEIVDHTGTEVTASKTSNQDLFYGFLGGSPGNLGILTHFTVEVQDDNNYQGSKGIGSSKVIASSLTPYAKHTGSTNSTNLSNNNWSAWFSRQIEKATKTKGLFVSSQIQVYGGNNSMFWKNASEATAYSWRDATVVGTWDVFYNNAQAAAEAWQKDTAAGALTYFDSNERRLLWGSYDNWDMTQVWQHYYDRTTYQRLQQIRKTHDPNGLFTANLFCVQAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ28</accession>
    <name>A0A9W8XZ28_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein phosphatase regulatory subunit Sds22</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">sds22</name>
        <name evidence="4" type="ORF">N0V83_009735</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ28"/>
    <dbReference type="OrthoDB" id="266138at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000446">
        <property type="entry name" value="Protein phosphatase 1 regulatory subunit SDS22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050836">
        <property type="entry name" value="SDS22/Internalin_LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND IQ DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652:SF3">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="27"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4363442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="381" mass="43410" checksum="C2B479E54493FCFF" modified="2023-11-08" version="1">MSERIPTPQSERDPATTSDATESQDQDGEHHKPRDSKGWDGKLRLDKMTLGDVPRDPRVEVESEPEESDDEGPPPEQLAADEDLLDDVPEDEDEIELVHCKVADMDSLRLERFKQMKRLCLRQNRIEAISIPNTAASTLTEVDLYDNLIAHIKGLEAFTELTSLDLSFNKIKHIKRLNHLTKLKDLYFVQNKISAIENLEGLTNLRQIELGANRVREIEGLETLTGLEELWLGKNKITEIKGLDTLSNLKILSIQSNRLRSITGLEKLSNLEELHISHNLLTDLSGLENNTNLRVIDISANPIEHLTGLKGLTHLTEFWASNCKFNDYAEIEKELRDKEELETVYFEGNPLQRSQPVLYRNKIRLALPQVVQIDATFVKQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZL7</accession>
    <name>A0A9W8XZL7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_010288</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NmrA-type oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZL7"/>
    <dbReference type="OrthoDB" id="9997102at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051164">
        <property type="entry name" value="NmrA-like_oxidored"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42748">
        <property type="entry name" value="NITROGEN METABOLITE REPRESSION PROTEIN NMRA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42748:SF25">
        <property type="entry name" value="NMRA FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="3">
        <location>
            <begin position="4"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4363168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="320" mass="35046" checksum="6CB824D6E46354DB" modified="2023-11-08" version="1">MSRTKAILVTGATGKQGGSVLKQLAKHPSSSEYMLLAVTRDANSASAKRIVEGHPAVKLVQGNLDDVPALFSAAKTALKEAGQEPKIWGVYSVQVSMGKNVTHDSEVAQGNALIDESIKEGVTHFVYSSVDRGGNKRSFENTTPIPHFQSKYQIEKHLLEKAGKNGENMGWTILRPVAFMDNLMPGFPTKVFLAALRDTLQGKALQWVSVEDIGIFGARAFREHEAWNSRAEGLAGSELTFDDMSACFERVIGSPAPATYGAFGSALMWAVTEVNLMITWFATEGYGADIAKLKKEEPKLCDFETWLRERSGWKSEVMLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y033</accession>
    <name>A0A9W8Y033_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Exosome non-catalytic core component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SKI6</name>
        <name evidence="5" type="ORF">N0V83_009592</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase PH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y033"/>
    <dbReference type="OrthoDB" id="437922at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF0">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="3">
        <location>
            <begin position="22"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="4">
        <location>
            <begin position="156"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4364137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="220" mass="23741" checksum="93A5158C229672ED" modified="2023-11-08" version="1">MPLDTSTYSLALLRLDGRRWNELRRIHAQISTQASADGSSYLEMGNTKILVSVTGPSEGSRQQQATQSRGGGAAGLAKVSVEINFAGFSGIERRKRKSDKRTSEMEHCLRRWRTACCINAATLALIDAGVPMTDYLVACTAASSASATAADNAAADDPLLDLNNQEELELPFLTVATLGESEGVAVCVLENRVRMERVEGMLAVGIEGCKRLRGDFGWRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y075</accession>
    <name>A0A9W8Y075_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009383</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y075"/>
    <dbReference type="OrthoDB" id="5421421at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="333" mass="37662" checksum="FF21058A56F1B1C1" modified="2023-11-08" version="1">MRYSGDSSVSATQVEPPPPAVSPVSAYSRDSAVLASTRSSEEPQPDLNAAAQRPIKHLRGQSHLVDRSPSSSANVRPAPTEASWAMDPLGIHSSHETYYSPFPTMTPSPRLSEPLLDQLPSAHLTSPQPRRMYTPIAPQPMEVPRSHGVKRAAAEDVIVDDVVSDEAAKRRKRSDSNTSSTMQQQFELSEEDKLLLQLKEEESMPWKDIAVRFQSDLGKSYQIPALQMRLKRLRERMRVWSEPDVRALRMAHEYWAQNKFDIIAQKMLDFGAQEKWTARQCARKWAEIEPAPTPYAAHYEQQQQQQHLQHGFAPYAMSPIEPPHAFLSYMPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0W4</accession>
    <name>A0A9W8Y0W4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TIM13</name>
        <name evidence="16" type="ORF">N0V83_009799</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIM8-TIM13 complex is non essential and only mediates the import of few proteins, while the predominant TIM9-TIM10 70 kDa complex is crucial and mediates the import of much more proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterohexamer; composed of 3 copies of TIM8 and 3 copies of TIM13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIM22 and TIM54. Interacts with the transmembrane regions of multi-pass transmembrane proteins in transit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Mitochondrion inner membrane</location>
            <topology evidence="1 14">Peripheral membrane protein</topology>
            <orientation evidence="1 14">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the small Tim family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0W4"/>
    <dbReference type="OrthoDB" id="7813104at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042719">
        <property type="term" value="C:mitochondrial intermembrane space chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000001">
        <property type="entry name" value="mitochondrial import inner membrane translocase subunit TIM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0143">Chaperone</keyword>
    <keyword evidence="10 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 14" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="15">
        <location>
            <begin position="46"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4363504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="122" mass="13598" checksum="18F4B11A7DC7F9CB" modified="2023-11-08" version="1">MDSLGASGMSAFPGDPYVKLPCHSVSMLRPALTDVRVCSKTQIMRQVQQEAAMQNARMLVEKLNEHCFERCVPKPGTSLSKGEETCFTSCMEKYMSAWNTVSKQYVARIQRESAQSSALQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0X9</accession>
    <name>A0A9W8Y0X9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cerato-platanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_009840</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cerato-platanin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0X9"/>
    <dbReference type="OrthoDB" id="4898945at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22778">
        <property type="entry name" value="DPBB_CEPL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010829">
        <property type="entry name" value="Cerato-platanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07249">
        <property type="entry name" value="Cerato-platanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040861999" description="Cerato-platanin" evidence="4">
        <location>
            <begin position="22"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4363544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="167" mass="17537" checksum="B66487D11C0C59BE" modified="2023-11-08" version="1">MMRLFSATAIAALGFAGFGSAQLMNVGYDTNYDDASLLVAAVSCSDGVNGLMTRYGWQLLGDIPSFPHVGAFPGVTWNSEECGNCYRFSYQEQFIYVLAVDAAYGTPVIAQLAMNELTLGKAASLGRVLANVTLVDSANCYLGETLPPTEPTVAGNKSVGSGQNVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0Z4</accession>
    <name>A0A9W8Y0Z4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Luciferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009648</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0Z4"/>
    <dbReference type="OrthoDB" id="5561043at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051260">
        <property type="entry name" value="Diverse_substr_monoxygenases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011">
        <property type="entry name" value="ALKANESULFONATE MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011:SF41">
        <property type="entry name" value="XENOBIOTIC COMPOUND MONOOXYGENASE, DSZA FAMILY (AFU_ORTHOLOGUE AFUA_3G15040)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="231" mass="25521" checksum="18B78CC583F9D7AC" modified="2023-11-08" version="1">MPQIHHKGKHFDVPGPHICELPPQRTPFLFQAGTSKAGREFGAKHAEAIFVGGQTPEIVRTSTDALRQLASEKFGRNPRHLKIIAGITIIVDDTDEKAQAKRAELLSYGDREGALALFGGWTGYDLSTYTDDEDFRFVKAPAIESIVNRWSATVPGTDNAPWTKARIAEYLILGGMGEKIVGSPKTVVDELERWLDVADVDGFNLSHVVSPGSFEDIIQRVLPELRKRGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y152</accession>
    <name>A0A9W8Y152_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cell wall protein Ecm33</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ecm33</name>
        <name evidence="8" type="ORF">N0V83_008813</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y152"/>
    <dbReference type="OrthoDB" id="536881at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.20.20">
        <property type="entry name" value="Receptor L-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051648">
        <property type="entry name" value="CWI-Assembly_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036941">
        <property type="entry name" value="Rcpt_L-dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31018:SF3">
        <property type="entry name" value="RECEPTOR PROTEIN-TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31018">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12454">
        <property type="entry name" value="Ecm33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040778066" evidence="7">
        <location>
            <begin position="21"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="352"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4365195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="393" mass="41112" checksum="27366055C65E57CB" modified="2023-11-08" version="1">MPSMLKIAVSALAAASTAYAASSSCSVSATTTIQNAGDATAVAACETFSGSIAVATGTTDDIALDGMKKLSGNLVATNNSAIKRISSSTLETLDGTLNLDGLTRLYGVDFPVLKNIDSIKWNALPNLQSIGFTAEVEEAKKVDIQNTGLRSLKGINIEEVDTFFVANNGYIDEISMQLGNVSESLTLADNNENVKVELPNLIWATNLTFRFCGSVSVPSLETLNGSLGLYNNGFETFSAPNLTSVGEALAIVANDKLDNITFPMLKKITDNLQIANNSALQEVSGFPVLESIGGAFDMSGNMSTVETPKLDDIKGAFNLQSSDNVTAACNFYNSLKSKKLIQGKYFCRGKVEDPGTEGHKPTDQSGTSTSGASSLSTATGALGLAAMAAVLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y164</accession>
    <name>A0A9W8Y164_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010304</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y164"/>
    <dbReference type="OrthoDB" id="4590138at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39474:SF1">
        <property type="entry name" value="FUNGAL SPECIFIC TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39474">
        <property type="entry name" value="UNNAMED PRODUCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="111" mass="12142" checksum="808598520AD5E396" modified="2023-11-08" version="1">MPSSTTSPNIPEQVPEPNQPSTSSGSSQQPLPLPEPSSSQEETTKVDINGEGVKLDHLGPLVVNKDGTLSRITNWATMAEIERQNTLRILGKRNQLRRESLKKEQGGREGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y182</accession>
    <name>A0A9W8Y182_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y182"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42678">
        <property type="entry name" value="AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42678:SF2">
        <property type="entry name" value="AMIDASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G14410)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="2">
        <location>
            <begin position="28"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="445" mass="48109" checksum="7413E71CDFB31A06" modified="2023-11-08" version="1">MTEPFHLSASQAQIKFNDGSLTVEEYAKSLLSRIEARDSAVHAWAHLDPELVLQQARQLDQIPSEKRGPLHGVAVGVKDVILTKDMPTQHNSPIYDGDAPKIDAASIAVLRANGALIFGKTTTTEFAATSVGPSHKGPKTANPHDPSRTPGGSSSGSGAAVGDFQVPIGLGTQTVGSTIRPGSFNGIYAMKPTWNSISREGQKLYSLIFDTLGLYARSIDDLKLLLDTFQLVDDEPEEKFEVKGGTFALLTLPTPDWPEPGPGTAAALKKGAELLRSHGAIVEEITLGDEFRPMYKYQMQVLTGDGRVAFLPDYYTAKDKLDSYLVGHVENHGKHTRKDQLKAFDELAAMRPKLDALVDKYAAIIAPSALDEAPVGLENTGNAAFCGPWTAMHMPVVNVPGFQGEDGLPIGLSVVSSRYRDQHLLRVSREVGKIFEAEGGWTRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1A2</accession>
    <name>A0A9W8Y1A2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="8">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_009361</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the peptidase C12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1A2"/>
    <dbReference type="OrthoDB" id="427186at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09616">
        <property type="entry name" value="Peptidase_C12_UCH_L1_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.532.10:FF:000006">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.532.10">
        <property type="entry name" value="Peptidase C12, ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001578">
        <property type="entry name" value="Peptidase_C12_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036959">
        <property type="entry name" value="Peptidase_C12_UCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589:SF17">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01088">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00707">
        <property type="entry name" value="UBCTHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52048">
        <property type="entry name" value="UCH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7 8" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UCH catalytic" evidence="9">
        <location>
            <begin position="10"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="site" description="Important for enzyme activity" evidence="7">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS52048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4363909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="242" mass="26632" checksum="D4DE9CEE48C52C66" modified="2023-11-08" version="1">MTSTQRHRKHFIPLESNPDVFTELIHELGLSKSVSFQDVLSLDDPELLAFLPRPAYALVLVFPTTEAYEERVKTKEAEVDEYRGSGAGEDVVFFKQTINNACGLYAILHAVCNGEARGMNGDNSLLGRLLEASVPLAPNERALVLEGSKELEVAYAVVARKGDTEAPDSAEDEVDFHYVAFVKSHNNKHLYQLDGARKRPIDLGPLAADEDVLSKKCLDVIRSMIASESNNPNFSLMALVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1R9</accession>
    <name>A0A9W8Y1R9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009902</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1R9"/>
    <dbReference type="OrthoDB" id="3933435at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="351" mass="39337" checksum="BEB0BCDB3C51B102" modified="2023-11-08" version="1">MCNVIAFRFTCAHTLYRRRSSCSGTKHKVTRTSVKAACTAESFLTLTLPYDCGPCQRYHWESNWKSKLSRANSFLHKLEERRSLLLPAESEEVIRGLVKQLEDQYTSEAWGTRNVFAEEHKRSVARVRVGEYRRIQSLLSREVRAEDVVEEGEGKEWALMGVSDYDGDYEASTDPIHPVSTDYRHPWDDGDDGKWVLEQLGEGEEVESSDEVDTIDFAKSCWSWGDDDGFAEAMEKATPTSSNEQEKKQDREDRAVDLPNAIQEGTWGDESLIAWGPEAEASSSTSTAYINMNGHGNTHTDDAIRKAQVEEVIAAFWRAVNPAPTMDNSNTAKKPQPPPLPPQEGGQSAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1W0</accession>
    <name>A0A9W8Y1W0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ADP/ATP carrier protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ANT1</name>
        <name evidence="13" type="ORF">N0V83_008316</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1W0"/>
    <dbReference type="OrthoDB" id="446044at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015217">
        <property type="term" value="F:ADP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052217">
        <property type="entry name" value="Mito/Peroxisomal_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939:SF1">
        <property type="entry name" value="MITOCHONDRIAL THIAMINE PYROPHOSPHATE CARRIER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PMP34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="10"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="119"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="212"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4365696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="338" mass="36717" checksum="5E25E0C4D146C26A" modified="2023-11-08" version="1">MAGQSKVETLPPWGNAVAGAAGAVIANTLVYPLDLIKTRLQVQIKRSKTSNDPNPADHEHYDGAVDAIKKVVAQEGISGLYAGMAGSLLGVASTNFAYFYWYTVVRSLYIANRSLTASPGTAVELSLGAVAGALAQLFTIPVAVVTTRQQTMSKEERKGMIATGMDVINGEDGWTGLWRGLRASLVLVINPSITYGAYQRLREVLYPGKKTLKPWEAFILGSLSKILATIATQPLIVAKVGLQSKPPPARNGKPFKSFTEVMQYIIQHEGPMALFKGIGPQILKGLLVQGFLMMTKERIELSFILLFRYFRQIRAEKLQKLANIAAEKAGRSAPILVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y214</accession>
    <name>A0A9W8Y214_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008944</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y214"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="64" mass="6981" checksum="A61721E19BD83C5A" modified="2023-11-08" version="1">MPDFGDVLDPAETESGEADFDGDADEEEAEMSQSEADAYGHIAQAETRQQVRFDTQSGYIPNPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y227</accession>
    <name>A0A9W8Y227_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008769</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Cyclase 1 superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y227"/>
    <dbReference type="OrthoDB" id="5396at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.50">
        <property type="entry name" value="Putative cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007325">
        <property type="entry name" value="KFase/CYL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037175">
        <property type="entry name" value="KFase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34861">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34861:SF8">
        <property type="entry name" value="CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04199">
        <property type="entry name" value="Cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102198">
        <property type="entry name" value="Putative cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="38648" checksum="69AA4A374843F6C8" modified="2023-11-08" version="1">MATNRGVEEARALHAVAFDSLPDYRQVWNKAEAGSHTEGLGRLVIITDDMIAAAARQCIRLGRRTTLAWDMNQLDVANFNRQPAQHHIVGLLGGLAFDDVYVFNPQQSSQWDGLRHFSAPFPVQSESRLYYGGVPAKEIMDRSSDRIGIQHWAQEGICGRAVLLDYARYAKRHEINYSCFSDHSISLDTLLDVAKEQNDLQFLRGDILLVRIGLVNEWEKEMGTTERMAYATTESPQHAGVEGTMEMLRWFWDTGFAAVGGDSLSFEIYPPQKEYRRGGTSPTVPGLFMHEVLLAGWGMPIGELFDLEKLSEICAEEGRWHFFLSSAPLNMPGGVSSPPNCIAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2H5</accession>
    <name>A0A9W8Y2H5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Translation machinery-associated protein 16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TMA16</name>
        <name evidence="3" type="ORF">N0V83_008152</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TMA16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2H5"/>
    <dbReference type="OrthoDB" id="270284at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.170">
        <property type="entry name" value="Translation machinery-associated protein 16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021346">
        <property type="entry name" value="Tma16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038356">
        <property type="entry name" value="Tma16_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349:SF2">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11176">
        <property type="entry name" value="Tma16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="179" mass="20809" checksum="FEB1C626A96CE6A0" modified="2023-11-08" version="1">MVSNRLNKVHKQITKKKGKSPNLHENSRDTQRLQRAAGRDDKINRLGKVREKQNRTYLLRIKSFQQFTTEHSSTLSIPEIQAMIEDYLGRDDEELAKLKADRRPGRPPMTRQTLLEQNQVVEQGEYASGFWVPDLEDEANLRKLKEWNGQWANLATLKFARISKEGFKRDSSFPPKGMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y327</accession>
    <name>A0A9W8Y327_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Para-hydroxybenzoate--polyprenyltransferase, mitochondrial (PHB:polyprenyltransferase)</fullName>
            <ecNumber evidence="10">2.5.1.39</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">COQ2</name>
        <name evidence="10" type="ORF">N0V83_008846</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.39" evidence="10"/>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y327"/>
    <dbReference type="OrthoDB" id="18170at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008412">
        <property type="term" value="F:4-hydroxybenzoate polyprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13959">
        <property type="entry name" value="PT_UbiA_COQ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.140:FF:000008">
        <property type="entry name" value="4-hydroxybenzoate octaprenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1780:FF:000001">
        <property type="entry name" value="4-hydroxybenzoate octaprenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039653">
        <property type="entry name" value="Prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048:SF28">
        <property type="entry name" value="4-HYDROXYBENZOATE POLYPRENYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048">
        <property type="entry name" value="PRENYLTRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="85"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4365228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="286" mass="30835" checksum="EBC914317C4FD313" modified="2023-11-08" version="1">MATPIAAPSAVIGTTALFFTGALVMRGAGCTINDLWDRNLDPHVERTRLRPIARRAITPKQAIVFLGGQLTTGLAVLLSLPFECFWYATPSLLFVALYPLAKRVTYYPQFVLGLTFSWGAMMGFPALGIDLLSNQTALTAAACLYASNVAWTVLYDMIYAHMDIKDDAKAGIKSIALKHEKETKAVLSGLAVVQLGLLATTGFVAGLGPVFFIGSCGGAALTLGTMIKRVKLKEVKNCWWWFVNGAWFTGGAISLGLAGEYVTHLVGWYDEGEKTGEKTEQNLVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3B1</accession>
    <name>A0A9W8Y3B1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-galactosidase</fullName>
            <ecNumber evidence="3">3.2.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_008136</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 35 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3B1"/>
    <dbReference type="OrthoDB" id="1657402at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.102.20.10:FF:000001">
        <property type="entry name" value="Beta-galactosidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000040">
        <property type="entry name" value="Beta-galactosidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.20.10">
        <property type="entry name" value="Beta-galactosidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.390.10">
        <property type="entry name" value="Beta-galactosidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018954">
        <property type="entry name" value="Betagal_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037110">
        <property type="entry name" value="Betagal_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025972">
        <property type="entry name" value="BetaGal_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036833">
        <property type="entry name" value="BetaGal_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025300">
        <property type="entry name" value="BetaGal_jelly_roll_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015305">
        <property type="entry name" value="DUF1961"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019801">
        <property type="entry name" value="Glyco_hydro_35_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001944">
        <property type="entry name" value="Glycoside_Hdrlase_35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23421">
        <property type="entry name" value="BETA-GALACTOSIDASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13364">
        <property type="entry name" value="BetaGal_ABD2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10435">
        <property type="entry name" value="BetaGal_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13363">
        <property type="entry name" value="BetaGal_dom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09224">
        <property type="entry name" value="DUF1961"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00742">
        <property type="entry name" value="GLHYDRLASE35"/>
    </dbReference>
    <dbReference type="SMART" id="SM01029">
        <property type="entry name" value="BetaGal_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117100">
        <property type="entry name" value="Beta-galactosidase LacA, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01182">
        <property type="entry name" value="GLYCOSYL_HYDROL_F35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040939776" description="beta-galactosidase" evidence="8">
        <location>
            <begin position="19"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-galactosidase" evidence="9">
        <location>
            <begin position="546"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4365517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1168" mass="128430" checksum="86031AEE5043B94F" modified="2023-11-08" version="1">MHFSNLSVFWIMVSMVSAEKLLYRNTFNSSDTIASWISEGPLKATVSNNTLELAGVGGPDDYFVYWLPQIFPDRIRITWEFSPIQEPGLAMFFFGATSVAGGSIFDKDLKPRNGSYPQYHSSDIRLLHASYFRRRWPEERAFSLANLRKSPGFNLVAQGADPLPPVADAKSAFYKVEVVKDKRDVSFSINGLLLFSFDDTKTDTGPVVREGRIALRQMQPLIARLPVPSLWLDVFQKIKALGYNGVSFYAAWVLHEPKPGSFSAEGIFDWEPYFDAAKKAGVYLVARPGPYINAEVSGGGFPGWLQRISGNLRTPDQDYQQASKNYIESITPIIAKAQITNGGPVILFQPENEYSMGLNNVTFPDASYMNGLMQQFRDLGIVVPFINNVAWANGVNAPGTPAPVDIYGHDGYPLGMNCTDPTCWTDDALPTNWRETHLEQSPNTPYLLAEFQGGAYQPWGGDGFEKCAEFTNHEFERVFYKNNIAAGVTISNVYMIFGGTNWGNLGHAGGYTSYDYGAAITEERQVHRQKYSEAKLIANFVHASPALASAVPGYNTTGVYTDTKAITVTPLFSNRTSFYVTRQTKYNSFGSTAYKLKVQTSAGNITLPQMGGELSIIGRDTKIHVVDYDVGGFNVLYLTADIFTWKRYDTRSILVVYGDQNEVHELAVSKAGGATAVEGSGVKFANRNGNTILHWKTTSSRRVVRVGNDLYIVILDRNSAYTYWTVSTLPEGSYTHDAAQSSDIIVKAGYLIRSANIDSGYINLVGDINATTTIEVIGGAHKNITGITFNGNAVEATLDRNGFLRGTVAFSAPAFTLPNLDSLPWKYIDALPELKSTYDDSAWTDADHVETNNTYWPLSTPTVLWGAEYGYNGGSLITRGHLVATGNESVVSLNVSGGNAFAFSAWVNETWLGSWPGTSDAAIANLTFPLPILKRGNNYVLTVLSDHMGHNGNWFIGYNEMKTPRGIVGYDLLGHSASSSNTSRTKDGITWKITGNFGGEDYPDRTRSPLNEGALWVERNGFHLPSAPTESWTDSAGPTAGLSKPGVGFYTTSFALDIPYDWDIPLSFVFHGDAFNGEGKGWRAQLWVNGYQFGKFVNGIGPQQRFPIPEGILNYRGENFVAVSIWALESGGAKPDRVELVSGMPVKSGYGEVQIPPMSSWVKRHGVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3I9</accession>
    <name>A0A9W8Y3I9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription factor Bmr1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_008443</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3I9"/>
    <dbReference type="OrthoDB" id="40579at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47660:SF2">
        <property type="entry name" value="TRANSCRIPTION FACTOR WITH C2H2 AND ZN(2)-CYS(6) DNA BINDING DOMAIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47660">
        <property type="entry name" value="TRANSCRIPTION FACTOR WITH C2H2 AND ZN(2)-CYS(6) DNA BINDING DOMAIN (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="2"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="78"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="110"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="165"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4365822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1016" mass="113148" checksum="3A6FAA8EAE8424FE" modified="2023-11-08" version="1">MVFCTYCGQSFTRDEHLERHILTHTNVKPFKCFTCHMSFARRDLLQRHYTVHGRNQNNEEGLPPQGIIPKSAGRTPIACSNCAKTKTKCDKKFPCSRCAQRNLKCTLRPTRRASKGVQRVGGPPEGVSAESAGSSSENGSAAEPLTTSGSTSPSQEVQAQQANTQPSPLQLQQPQQLPIGQTQSRRQSQSPSISHSRNPSISQPVQPLSHATPEEKTVHISLSHTPPFFDQSPANVIPQAPSLMSPLPTSVNGMNSFVSSTPMSGYDDFVRTVRDQPENESPQFMMDPWNTMGMGTEFDPMRIDPALMMTMSMDMSMGPPPDGILGMMPEMTPPQAPFGPVQTPIQTPRMDESFSDLQIGSSASMFYPSNRHSSIADAGIPDLGAIIAAQDGWTVFRCTPSIPSSSCPRTAKMNLERLELSLKNHEGWSSWTPSWDESDYQQGGQIAVAKMQEPTRDKLLAITQSFLHKALDTHKDGMSTSSSSGESPGSMASGSNFVLLPPARVLEYFLKSYANSFERYYPTSSRGILDTNELMQNYNDKASSLLILMMIAQGAMTIPSIEARWLTGGFTEACRISLFDLIERNIIMAGDPIVLRAALLFTVQAAWSGDKWQMDIAMGQRGMYFAMLRHSGILEPRSVATPHMNGNSTTDALWRDWIQQESRSRLIYSWVMVDQDLSLFHDAAPLFSVTEFGAPMPDSDRLWQAQSAVEWSEMFNQVHEFSNGYSSVGSGARPPSLRDLFRHFLDDEIVIQDVQEIHLTPMHLRLLLHPLQTLVCQYCQLLSCFSDSVASRSRNRALTAASTRVRLEEVQALLGRWFDLAQRYMKSNPICPMMQGNLVMFHLISMNAVTNFPEIERLARREVVDGSYQQMLWMHKKCLSDVQEAVTHAGQILRLIREMPRGIRPPWWPGAVYRAALVLWTDSLTQNESINSNQQGAYQSPNAAFSVDRLPADHQTILRFLSRREGIPTLTKRDGTPVPLDNGFAVLSHCIDVIDEGLATRFSDGIRSKLEKLARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3Y5</accession>
    <name>A0A9W8Y3Y5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008453</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3Y5"/>
    <dbReference type="OrthoDB" id="408373at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052897">
        <property type="entry name" value="Sec-Metab_Biosynth_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37017">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37017:SF11">
        <property type="entry name" value="ESTERASE_LIPASE_THIOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040862554" description="AB hydrolase-1 domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="32"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="286" mass="31286" checksum="1A1D6AC235A0F0B9" modified="2023-11-08" version="1">MASYKALVLLVTLFLSIFSSAEAQTAPDKPAIILVPGAFHKASVYDQVKQLLNEVDYNHIDAIDLPSVGSLASYVDREPDIIAVRLALLARLLQGHNVVLVGNSYGATVIGEAVSGLQSVGKLASGPSPPGRILGLIMLSGFIPYITEVSQPDSRPDIRVISPSWFRFENTDRVYWNGNTTAFPPQKTFYDLLPTTQADYWSCKLVPSSFHALNATATYIPYTGDFRCLFVIGEQDNSVPPAFAQTYIDQPGAKFETLTIDGDHVPMLSRPDVTADIIRRFAGEKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4A6</accession>
    <name>A0A9W8Y4A6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4A6"/>
    <dbReference type="OrthoDB" id="3780939at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="95" mass="11322" checksum="E7B65597CB1CC7E6" modified="2023-11-08" version="1">MSDIAIRSIKSWVKKWGVGKRKRLGTEVEERVKLRVQELYVEYDLQDSEILLHILRKEGFDVSKWTPLRLKDDLGLERKPSRPNKKLPHELLTER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4L3</accession>
    <name>A0A9W8Y4L3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4L3"/>
    <dbReference type="OrthoDB" id="20282at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039146">
        <property type="entry name" value="GPANK1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20923">
        <property type="entry name" value="BAT4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20923:SF1">
        <property type="entry name" value="G PATCH DOMAIN AND ANKYRIN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="1">
        <location>
            <begin position="135"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="242" mass="26650" checksum="5E6D6A9C0C3A45B9" modified="2023-11-08" version="1">MAAPTMNPRHEEDTLEDMFEFADDPRYDGSDISTVPFVEQPAFGRGLWKKPVSFVREGAERPSPTKGLTPDGRTMAEAYAAIFLPNGPPQPKPPAGPLCGVCGCPVTEKDQRDHYLSLSHQLALPRAPIPSAIDRNRMGLKYMEKHGYDVDAQKGLGATGEGMLYPIVPKEKRDKFGLGINVEEHTKKKALGGASAADVKQGKLDAGKVRKLAAVEKKRHEKLQRAFYGNDEVEKYLDRLHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4T1</accession>
    <name>A0A9W8Y4T1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4T1"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="76" mass="8338" checksum="8C7C0E716FEFEC69" modified="2023-11-08" version="1">MPTSEPMIIAQQNLDNNAPTVPLPPATVAWPRAPQTVNSVENPLPENEVAAGDSDEYEDDTNINEEGDDFDDPRFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y517</accession>
    <name>A0A9W8Y517_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Inosine triphosphate pyrophosphatase</fullName>
            <shortName evidence="13">ITPase</shortName>
            <shortName evidence="13">Inosine triphosphatase</shortName>
            <ecNumber evidence="13">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="13">NTPase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">XTP/dITP diphosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">HAM1_2</name>
        <name evidence="15" type="ORF">N0V83_007627</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Pyrophosphatase that hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triphosphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ITP + H2O = IMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:29400"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-hydroxy-dATP + H2O = N(6)-hydroxy-dAMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:83971"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:233529"/>
            <dbReference type="ChEBI" id="CHEBI:233530"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:83972"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>XTP + H2O = XMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:28343"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="13">Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 13 14">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="13"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y517"/>
    <dbReference type="OrthoDB" id="6288734at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009204">
        <property type="term" value="P:deoxyribonucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.950.10:FF:000003">
        <property type="entry name" value="Inosine triphosphate pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03148">
        <property type="entry name" value="HAM1_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027502">
        <property type="entry name" value="ITPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="RdgB/HAM1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00042">
        <property type="entry name" value="RdgB/HAM1 family non-canonical purine NTP pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067:SF9">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE/HAM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="15"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="71"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="147"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="173"/>
            <end position="174"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00093218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00093255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4367097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="191" mass="21136" checksum="86BB9AA2AEDC3FE7" modified="2023-11-08" version="1">MTSTPTVPAHLNFITGNKNKLAEVQAILEGVIELRNQNVDLVEIQGTVEEVTLDKARRAAEAIQGPVLVEDTCLCFKAMNDLPGPYIKWFMLSLGAPQLHKMLAGFDDKSAQAVCTFGYCEGPGHEPVLFQGRTDGKLVESRGSTVFGWDSCFEYNGQTYAEMDKSEKNKISHRGKALEKLKEWLAQKVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y539</accession>
    <name>A0A9W8Y539_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Polyketide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PKS1</name>
        <name evidence="15" type="ORF">N0V83_008442</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Non-reducing polyketide synthase; part of a gene cluster that mediates the biosynthesis of a yet unidentified natural product.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pantetheine 4'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:47942"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.366.10:FF:000002">
        <property type="entry name" value="Probable polyketide synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.10:FF:000011">
        <property type="entry name" value="Sterigmatocystin biosynthesis polyketide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.110:FF:000001">
        <property type="entry name" value="Sterigmatocystin biosynthesis polyketide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000031">
        <property type="entry name" value="Sterigmatocystin biosynthesis polyketide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000116">
        <property type="entry name" value="Sterigmatocystin biosynthesis polyketide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049551">
        <property type="entry name" value="PKS_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030918">
        <property type="entry name" value="PT_fungal_PKS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032088">
        <property type="entry name" value="SAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001031">
        <property type="entry name" value="Thioesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04532">
        <property type="entry name" value="PT_fungal_PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF37">
        <property type="entry name" value="SI:DKEY-61P9.11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22621">
        <property type="entry name" value="CurL-like_PKS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14765">
        <property type="entry name" value="PS-DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16073">
        <property type="entry name" value="SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00975">
        <property type="entry name" value="Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="13">
        <location>
            <begin position="371"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="14">
        <location>
            <begin position="1290"/>
            <end position="1601"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="12">
        <location>
            <begin position="1663"/>
            <end position="1740"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="12">
        <location>
            <begin position="1795"/>
            <end position="1869"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="10">
        <location>
            <begin position="1290"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="10">
        <location>
            <begin position="1454"/>
            <end position="1601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1745"/>
            <end position="1782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1746"/>
            <end position="1759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1760"/>
            <end position="1779"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="10">
        <location>
            <position position="1322"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="10">
        <location>
            <position position="1514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4365821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="2170" mass="234553" checksum="7FEECA04F1F4ABB7" modified="2023-11-08" version="1">MNLLIFGDQTADQYALLRKACTWKNNATLTTFLDRISVVIREEAQKLPRTQRDQIPDFLTTWDLIEAYYASGAKLSPLESCLVTVAQLAHYIGYYSENPSELPNPANTRVVGLCTGLLAGTVVASARSLSELLPLATEAVRIAFRAGTCVGIVKDALDQTSNAKESWSTIVTGISENDAKNALAAFHDERRIPPSAQAYISAVSVMALTLSGPPSTTKRIFAESDAFKNNTRVPIPVFAPYHAPHIYGQADIDRILDKDAVRHLQQFRPLALVHSATHGRCQTETSTLDLMRVALNEILLEQVRWDSLLEEVVSQVTAAGSQKCAVFAIGVTNVANSLASALKAGGQTSISLHDQSSWVSTTDASRGRTQNDKIAIVGMSGRFPGAANPDKLWELLEKGLDVHREVPADRFDAQAHCDPSGKGKNKSHTPYGCFIDEPGLFDPRFFNMSPREAAQTDPMGRLALTTAYEALEMSGYVPNRTPSTKLARIGTFYGQTSDDWREINAAENIDTYFITGGVRAFAPGRINYYFKFSGPSYSIDTACSSSLAAIQLACTSLWAGDCDTACAGGLNVLTNPDIFSGLSKGQFLSKTGSCKTYDNDADGYCRGDGCGSVVLKRYEDAIADKDNILGCILGAATNHSAEAVSITHPHAGAQEFLYNKVLSNAGVDAHDISYVEMHGTGTQAGDGIEMTSVTNAFAPRHRQRRPDQTLHLGAIKANIGHGEAASGINSLVKVLMMMKKNAIPANVGIKGVMNKTFPKDLLQRNVHIETTQVAWPRKGAEKRKMFLNNFSAAGGNTAVILEDGPLRDAPKGTDPRSMHMVTVSARSIASLKKNISNLMQFIDENPETTLPSLAYTTTARRIQHNYRVAVCVSDVSKVKEALQAQLKDSYSPLPMVPTKTAFTFTGQGSQYTGLGQKPYQDLESFKADIDQLDNVARLHSLPSFLPLLTGADVATLSPVVVQLGMACIQVALARMWASWGVQPTAVIGHSLGEYAALHVAGVISVSDMVLLVGRRAQILEEECTANTHGMLAVKGSAESIRAALGDKMTEIACMNGPEETVLCGTVEVVASTSELLSEKGFKATKLNVPFAFHSAQVEPILEKFKDAAASVIFSKPVVPVMSPLTGQVIREAGIIGPEYLARHARETVDFWTALSAGQDDKLFDAKTAWVEVGAHPVCSGMVKSSLGGAPCTAGSLRRSEDPWKTLSNSITTLYLAGVYFDFNEYHRLFNDAHEMYTLPTYAFDSKRYWLDYHNNWTLTKGEVLQAAEPKAIEAAPVEEAPSKLSTTSCHKIVREDLHANSGTVVVQSDLSDPKLQATIMGHKVNGSPLTPSSLYADQAMTIANYLYQQLRPGMTTPGLNVCAMEVTKTLIPQYPPPAAGQHLQIEGTADLELNQMSIKFRTVTADGSKILVEHAAGIVKYEDVEAWKEEWSRIQYMVQSQIDMLKMKLETGAAHKVLRGMAYKLFKALVSYADNYRGMEEVILDGKQTEATAQVKFQTTAADGEFHCSPYWIDSLCHLSGFIVNASDHLDSETSVYISHGWGSVKFAEQLSPEKEYRSYVRMQPAPGNISVGDVYILEGARIIGVVSGLKFQNIPRRAFNIMMPPPGGKAAAGSAAKPVARSTPAKALPAAPIKAQVAPVKAAKPAAKVLVKPNKPAKLVKAVNTGVTAKVMKIIAEETDVDMSELVEEAAFENLGVDSLLSLTISARFREELELDIPSSLFTDCATVGELKGHFAQYDGGVTVVEDDDSSDTTDEPSDSSTPYEPAQASTPASSAPSIADNDIEVKVTSEIVEGEPSVARKLVADEMGVDVSEITDDLDLTDIGMDSLMSLTILGSMREQTGRDLPADFLTVNVTIKDIETALGMRPQPKQQVKVSAKVAPKVPHKSPQLAEVNKKLAKVIDVSHLPPSNSVLLQGNPKVATKKFFLVPDGSGSATSYISIPNISPDMAVYGLNCPFMKCPEKWSCGVEGVSALYLQEIKRRQPQGPYIIGGWSAGGVMAYEVAQQLVNAGEKVESLVLIDAPCPVALAPLPARLHIFFDQIGLLGTGKPGGTPSWLLPHFASAIQNLKDYEPTPMDPKIAPPVLAIWCTDGVCPNPDDPRPPPGEGEDPAPMKWLLNNRTDFDDNGWAQLLPKENFDYAVMGGNHFTMMKGDHGVTLGKLIKKGLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y565</accession>
    <name>A0A9W8Y565_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006998</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y565"/>
    <dbReference type="OrthoDB" id="3553147at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF64">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26639">
        <property type="entry name" value="Het-6_barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="49"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="616" mass="70570" checksum="7DFA3FACDA81E9B3" modified="2023-11-08" version="1">MSEGKNYYHYQSLPTQDHIRIIVLNPGSDHDPIQCTIDTVPLDEAEGTYEAISYVWGNPDDVTDIECDGQRISVTVNLADALRRFRYASEYRRVWSDALSINQRDVIEKGLQVKRMGLIYEKSLRVLCWLGVDGEGMAKDCFDLVREANRQITQQWNEEWTSKDYHTKVQLTNLMSDESRWHKFRDFLDLPWFERVWVVQEAGLSRECYLFWGKHQIEYYEVLELASWFAYSFHAPRKDDGSRIGRTTSDVFIDIQCTYARLPPWKHKSPILNVRGRWTSVESKLFQDLLYCARDLKVTDERDIIYSSLGSALALDMDGDLMIPPDYNESLMNLHIRAARALILNPKEAPTVLLRVKHESDSDFNNVSYPSWVPRWTPHDAMTRPIPLTWGYEHHRKPYRASGGHSFEPSLMAGTQIVVTGCIFDTVNWASILLKANNLNSNVEHWDADLRNDKISAIEIVWRGLLGSSGRNGEELIEEVSLTMCRGRPDFANHVNDLLAYCDLVRKRINTGPRSPFPSPEPGPATASIAEQVITRCRNRRLASTENNRLALIPLVGRAGDVCCLVKGMTVPVILRQTNQATYKLVGEAYVHGVMDGELMPLLDSEKLKWEKVCLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5A5</accession>
    <name>A0A9W8Y5A5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_006881</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5A5"/>
    <dbReference type="OrthoDB" id="6019893at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032483">
        <property type="term" value="P:regulation of Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051696">
        <property type="entry name" value="DENN_Domain_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296:SF31">
        <property type="entry name" value="DENN (AEX-3) DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G11200)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03456">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00800">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="3">
        <location>
            <begin position="17"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="739"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="459"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="740"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="754"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="797"/>
            <end position="809"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4367300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="868" mass="97515" checksum="F2ADABB542403793" modified="2023-11-08" version="1">MVCGVGREPSQWVKAPAPAQGKIGHMKGAVGTFWLPEILGSSPRLEQDNEIARSLHAAMRACFPHDVEICTGRSQPHCVHHSFVLQQDSSHTLYGIALRVWSRADEKRAETIRDLRKRIEPDFFDNPDETYWIPYCLSFLSRYPLYNLLGDYLRGMWIHWNKATNLFHAEEVSRILSFPAPRLNDLVRIDMKDYALCYQFPSSPTGFQNFAMWPLFTCLSIPNIVGVIEAAVSPTRRIIFTSHYPAMLTIAAETVRFCVRVYEWSGLYVPVVHARHVKDLVQEPGPYILGVTSECRSLFTAPTDALVVDLDRNFVLTSSPPTALTAGQRTKMINRLTQALNGEVSPTGVPQHLRSAYGGGKLIPAGQIIVMRGEVESIQDPDWWTQDAVMTVMDHVCEKLGRNTGMKAIFGGSVKKPLMTKVSMRHLNEIVRERNQYSRDAQEAWQDFINLKGRMDTELSKVTKRNNFLVEELESWKQQFLKFQAFAEALTKETQELKVKIENHKRENRRLSNLIDQQKDDAARLTIRLSGTEKQRDDALEALVLQQEIAEELERERKKNKKELSALQHTNGAILRQRDEAQRVVLHLRALIEGQTHHMEHIVKSLNQDDMSSYIEAGFEDVPEEDEEEFQDADVDADVSAKDKSEVGTIRGVAGLDSRNASRASTVEGKHLEGEDVTPDMEQRLYSSPARNSKRFSNQSMVDVADRVLRDKTDAIAYIIRNISEQCAAAVEGLQLAQRADTEDGDNHSDRRSSVGQLSSAGGRAQSTAGSEYGDEDMLRPGRGSSIPPTPDLSHRSSTSMSMASASTTPDRTSLQHRSHDIPDVPTRILEDDDDVDGNSDLHADMVPVSKYATPLQRPGQAVRNAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y660</accession>
    <name>A0A9W8Y660_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005997</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y660"/>
    <dbReference type="OrthoDB" id="5386823at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="166" mass="17573" checksum="69388AED82E0938C" modified="2023-11-08" version="1">MSKNLMDTEIGGEQTQYEGAHTFVEPHNKQAGQLGGTQPPSSFLPLYIRCSYSSASFESVFTPIYKSLNLLRTDSSEGTSTTRNSSSTSGDEPNTQGVSKSDKIRYGQSIQEGGMGGKTASSTGEANAEGGFGGTQALDESDDSFAKERREQGYGGGKDMDRNVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6I3</accession>
    <name>A0A9W8Y6I3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-conjugating enzyme E2 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">E2 ubiquitin-conjugating enzyme 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Ubiquitin carrier protein UBC2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Ubiquitin-protein ligase UBC2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CDC34</name>
        <name evidence="11" type="ORF">N0V83_007506</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6I3"/>
    <dbReference type="OrthoDB" id="19692at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23811">
        <property type="entry name" value="UBCc_ScCDC34-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000063">
        <property type="entry name" value="CDC34p Ubiquitin-conjugating enzyme (E2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="10">
        <location>
            <begin position="4"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="175"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="183"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="203"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="7">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00039884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00041569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4366976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="231" mass="26468" checksum="29B454BDA467D97E" modified="2023-11-08" version="1">MAEAAQRVLMNEFKQLSKEKWTNIELVNDNVFEWSVALIVLNEESMYYGGYFKARMTFPRNYPHSPPDFKFIRPLYHPNIYPDGRLCISILHPPGDDEMSGETAAERWSPVQRVETVLISILSLLDDAEVSSPANVDAGVMLRNKRSEYMDMVKKDLELSKQDIPEGFVMPTHETAWKKKDDEGDFDMSWEDSDVESFGGSESDLDDDDEEVDSDFDGADDDVEDEDEDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6Q8</accession>
    <name>A0A9W8Y6Q8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_005834</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6Q8"/>
    <dbReference type="OrthoDB" id="10257314at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000003">
        <property type="entry name" value="Alpha-ketoglutarate-dependent taurine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051323">
        <property type="entry name" value="AtsK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468:SF28">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT TAURINE DIOXYGENASE (AFU_ORTHOLOGUE AFUA_8G02210)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="7">
        <location>
            <begin position="82"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="370" mass="41203" checksum="1B5D05D89706F49F" modified="2023-11-08" version="1">MAAVTTVQETTNADGEPIKLAVAYDDKVHNESKYSDYLPVYDTTTKFPPTELFDFEDRGHHADPAKPNLLPKTTNTGIKAIKLTPRIGTEITGLQLSQLSNVQKDELALLLAERGVVVFRDQDFKDIGPSAQNEFGQYFGRLHVHPVGAHVKDHIEFHSIYIGKDNLYRLGRSSAKLTTTGYHSDVSYEHQPPAITLLTLVQVPETGGDTGWTSQAAAYDRLSEPLKGFLEGLRAEHSGFPQAEKAARDGHFVRREPVKSYHPVVRVHSVTKQKVLFVNPGFTKRIVGLKDEESDAILKVLFRHIAASQDIQARVKWDDRTVALWDNRVTAHTAISDYDVNDEGEGLRQGFRITTLGEVPVGVNGLKSEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y772</accession>
    <name>A0A9W8Y772_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Disintegrin and metalloproteinase domain-containing protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_006018</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probable zinc protease.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y772"/>
    <dbReference type="OrthoDB" id="5951731at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04271">
        <property type="entry name" value="ZnMc_ADAM_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000003">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034028">
        <property type="entry name" value="ZnMc_ADAM_fungal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF159">
        <property type="entry name" value="ADAM METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13688">
        <property type="entry name" value="Reprolysin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040825489" description="Disintegrin and metalloproteinase domain-containing protein B" evidence="7">
        <location>
            <begin position="22"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="696"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="9">
        <location>
            <begin position="267"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="8">
        <location>
            <begin position="508"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="730"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="731"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="760"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="770"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="421"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00056552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00074021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4368936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="830" mass="88066" checksum="966D7C58C0A2D023" modified="2023-11-08" version="1">MRLLRSVTAAVTVALATLTAASSNARAPLRSIALAKNADILTPNHRATAVSTFDVAFDVSGQRIRLSLEPNHDLFVEGGRITHLNADGSIAKEDPIDRLQHKIYKGTAWLKRGNRWDRVGWARIGLQRDGLEPLFEGTFTVQHDHHHVKLSSNYKATRMDEDPDIDLRDDDFMVVFRDSDMGKWEEHAELRKRSGGVACPADGLSFNTQADHPVYASMRARDDGFFSSPVSSLFGKRQIDGQPSGNGAGVNLVSTIGSTAGCPSTRKVALVGVAADCTYVQSFDRDKNKTQSNVIQQINSASELFESTFNISLGLANLIVTEADCPTSQQQSTPWNQACSSSITIQDRLNQFSQWRGQQSDSYSHWTLLSTCNTGSAVGLAWLGQACTTGSQTNTGSSGEIVAGANVVVRTTTEWQVIAHETGHTYGAVHDCTSTTCADANTVNSQQCCPLSSSKCDAGEGFIMNPSTADGITRFSACSIGNICSALGRNSVKSTCLTNNKDVTTVTGQTCGNGIVEGDEQCDCGGTTGCGDNSCCDASTCKFKNNAVCDDSNEDCCKSCQFASASTVCRASVGDCDPQETCSGSSPYCPEDKHKDDGTSCGSGSLSCASGQCTSRDMQCKTVMGSYTTGNDTYACDNSNCMLSCASPEFGSNVCYGLQQNFLDGTSCTAGGTCKNGQCEGGSVGKEISSWIDRHLSLVIGLAAGLGGLLLLCILSCCWRSYRRRSKLKKYAAAAPPQAPPYRAAGGRSARNRGGPVVSPNGSGNPLMSQNGQQGMQQQQQPWQHADGLAPSPAQQGGQWHPPPGPPPGHHGQGIPSPPPLVHRSSVRYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y788</accession>
    <name>A0A9W8Y788_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y788"/>
    <dbReference type="OrthoDB" id="6509975at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963:SF18">
        <property type="entry name" value="ACID PHOSPHATASE PHO11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="233" mass="26572" checksum="C651A3D199D17CA1" modified="2023-11-08" version="1">MSRKDDSNLEQLTETGPFSGTLGSFTTGVRLKTRYEHLLPQTASRTTLKISLRPITFWASGSNRVVETARHFAHGFFGIDYKSRNTAALKIISEHHSLGANTLTPGRTCLANKRDVAEGQRKGYRLMGEYQATYLKAIRERLFRETSMKFGYQEIWAMQEMCGFETTVRGRSDWCDVFTQDEFLSFEYARDLLHYYRAGPGQRYAASMGWLWLNATTNLLLEGPEAGSLFFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7T1</accession>
    <name>A0A9W8Y7T1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mitochondrial splicing system protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MSS1</name>
        <name evidence="8" type="ORF">N0V83_005346</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7T1"/>
    <dbReference type="OrthoDB" id="188276at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04164">
        <property type="entry name" value="trmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14858">
        <property type="entry name" value="TrmE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.430">
        <property type="entry name" value="tRNA modification GTPase MnmE domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00379">
        <property type="entry name" value="GTPase_MnmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031168">
        <property type="entry name" value="G_TrmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018948">
        <property type="entry name" value="GTP-bd_TrmE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004520">
        <property type="entry name" value="GTPase_MnmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027368">
        <property type="entry name" value="MnmE_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025867">
        <property type="entry name" value="MnmE_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42714">
        <property type="entry name" value="TRNA MODIFICATION GTPASE GTPBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42714:SF2">
        <property type="entry name" value="TRNA MODIFICATION GTPASE GTPBP3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12631">
        <property type="entry name" value="MnmE_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10396">
        <property type="entry name" value="TrmE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="GTP-binding protein TrmE N-terminal" evidence="6">
        <location>
            <begin position="7"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="MnmE helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="G" evidence="5">
        <location>
            <begin position="240"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4369584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="522" mass="55966" checksum="C2F48B83E7AF7D76" modified="2023-11-08" version="1">MSYDEPTIYALSTSSGKAAIAVIRVSGPACKQIYQGLCPSTAFPKPRYATLRRLYSPHLPPSPATLLDSGALILYFPAPSTVTGEDVLELHVHGGPAIVRAVLAAIPGCAKAHRALQPQIRYAEPGEFTRRAFANNRMDLPQIESLGETLSASTEQQRKLSVRGTTSSLAGRYEHWRTLLLQARGELEALIDFSEDQHFDESPAALCASVADQVRVLRVQMEAHVANAVRGELLRNGISVALLGAPNAGKSSLLNRIVGREAAIVSQEAGTTRDVVEVGIDLGGWLVKIGDMAGLRRAGLVGPDIVGAVEQEGIKRAKQRALESDVLIVVQDATTEMDPEVMETAKRCVDLGIHVIVAINKIDQLETTHSAQEAWRGKIQPVLGILPERVVYISCKEASSPGSGNLPSFLDTLLDTFKDMTAALVPDSDPDPSIWQESLGATERQRVLLSECLGHLKTFLLAVEPRPTHSSQDLSTFGEASDGVDIVVAAESLRSAAEALARITGKGESGDVEEVLGVVFEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8B8</accession>
    <name>A0A9W8Y8B8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006826</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8B8"/>
    <dbReference type="OrthoDB" id="3795771at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="532" mass="59113" checksum="AE19BA374FEB435C" modified="2023-11-08" version="1">MDSKRSSRKILEAKGQSKRRVANMPEGRKKTTGRVQKPALDVPIRLPYELTPAQVRYAQGLAAIGTPSCPQYPNAELLERMRQKPAPPAHTAAPDLIPSRTPSSSGNDLKLPVPLYLSTPSQPGMRANWEAEALEVIRKRGFSLDNLWSANTWNMKSSREARLKLLKDDGYDITPLTIKGVTSAQITQAILGHIESRKQQIQAVTLPKQGMEKAVQDHGGVQQSTQLVDELHGRSKNRLQARRFKSSVPAKRKDCHEDEEMDQDAGTPTDGGSTPLPCSDGPALKKLCTLSPAERRKKIEIKPIKRNIFYASRIAQKSFTTVADPSPSDRSVPQSQMTPRSPIPASRIVFSPYDRSPRPLNLADVNIGSRGLRHMVDEMKYLSDTEDGHGVHYLLEPETSDSGKGYDENPSVMKSDVIDAAAEYSLQDKRYRHRISMAEGLQGGIVLQGDARALQGLLKHLRTGNLIHPKEWFFDGTDMVRRDIFPMIPFSLADGTKTVLAKVESMLERGQDEGGLVVRVGQIWDGRKLRWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8M3</accession>
    <name>A0A9W8Y8M3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Aspartic proteinase</fullName>
            <ecNumber evidence="5">3.4.23.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">APR1_3</name>
        <name evidence="5" type="ORF">N0V83_004470</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.25" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8M3"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05471">
        <property type="entry name" value="pepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000229">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034164">
        <property type="entry name" value="Pepsin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040880145" evidence="3">
        <location>
            <begin position="21"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="4">
        <location>
            <begin position="73"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="337"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4371253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="419" mass="46526" checksum="400C13AC0D0994E3" modified="2023-11-08" version="1">MISNEVYLVLFFALLPVCSGATATIPIKRTTIRFKFDDWQLYNEYAGAFQQPEAGHQHPLSRHDQSTFYHILYTTSIAIGNPPQPFTASIDLSWSDMFVPSVECLQDPVTAQYCRGQNKYNSSLSTTYHPSKTVTELMYEGLSTSGYLSQDVLGVGGLVILDQQFEEATFWRSMYLNAAPLDSALGLARLQSRTPASNASTKSALRNMLDQKVLSRPVFSLRLPRRDDESGELALGYSPLEVLAQSAVTLPLIEATPPKELSQLSYYISSGYTVALESVTIQSSPGNNPGGRKPSTYKAVFTNSFDYISLPGTLLTEIQTNLHPNLGMDDQLADLDCSMRDDLPNIVFSFGKNAVIVLTPWDYLTEVRDVEKGMRCVLPFAYLQQADDEWVVLGSAFMEGVYSVFDLEKGEVSLLMLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8U2</accession>
    <name>A0A9W8Y8U2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005837</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8U2"/>
    <dbReference type="OrthoDB" id="5296287at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053230">
        <property type="entry name" value="Trans_reg_galc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47654:SF5">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47654">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="2">
        <location>
            <begin position="296"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="692" mass="77702" checksum="BEF54BCDF4A7DDCA" modified="2023-11-08" version="1">MPRKDRLKTVTEHARQMAELLRSLSPRVSTQEKARIADILEAVDQELCEIRDSTTSSAADNDTYFSQKSTDPSLVNHHELHTQSSDFLDEDLHKNECTRATGFVGRASEVHWLRAVTLAQTEYEFDNMGDTTPHGNVPSESGSNQMMTSFSYWADSESVDIEFFVVPYELPPMETAERLLQCYMLKVHSSFPILQRKIFEEQFKRYFAVLQNGNAPRFSPKWQAILNMIFAIGAKYSHLANVSWQADERDHLIYQARARAFGLDESLVTAHPDLPTIQSVGLLSFYWLSVGQVSRAWTVVGIAIRFAYSLGLHIRSEDPSLEADKRETLARTWWSLYSLERTLSVVTGRPSIIVDSFCSVPLPIALPEDDVPNIVEATYPTRNDWTLTTATTNSGSHLKAAIQLSIITQDILTSLYSTGIMFRPPGEVQQVMTQLSQRLDDWIISLPEQFNFQLTSKASGVEFNRERMLLEFQFCSATILLTRPCLGGGRQTWSDAQETSFVKRMADSCIDAAKRIVSRLSEKPTPTLVLNDGPWWCIIHHTMQAISVFLLGLACPSSISHDSMTLVHNLKRAMRWLQSIRNPVAERAYSVALDAFESVARRCSVDVSASWVEDIEVLRERGAHHGVSIIASASELPDHFDTTCIAHAGGAATALASHVAYDVVMTESSFHLPRAAPIYEDDYYRDGNIGYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y914</accession>
    <name>A0A9W8Y914_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006166</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y914"/>
    <dbReference type="OrthoDB" id="3675887at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="475"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="581"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="488"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="508"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="636" mass="70273" checksum="4C7356F07C5E1B39" modified="2023-11-08" version="1">MFSSTSTEHLLNSFPMFGHPGISALEYAGSVEATQDSQIVDSYIGDKRLTINPGSPIELDATETGSPSSPEVYRPSTAIHRRSVSVPMSSIVTYQPSLTASAIHYDKDISVRSVSNPIQKPQEKKRQRRQTPFKIAIPKATNPARHAPLEPLVAQHRRKASTDTTSDLPLHPDTGKVEEMGTFGVIQRYFDSQTGGPVVSPKLLCHTCSQDPSTIPLPLSPKPSVQTPFKEPIAIYPIDELDFPDEPPAVPERSPKRLTNPAFPIHVKTTMSMSSDFAIAAEGQYSPYDKQDDVLHVPKRREPTRIDVGQAAWVGSSHIGRMAPPILGHDALTASSELRLNDLSFYLKNTGPSSDSQTAVRQRKKRGVKIFKAQKRKSLAARVGSVEGSPQRARMQNPVPACAREMTTSSGARHRKIIIPTDRMSGKQLIALPASQPDSQRRSRHVSITFTEEMLNPLASSEVERMLSDFQTPIGRSFTEPISQSPRSPKRQPKSPKRVPVSVNDDPLASREEQTRARKLRDLQLIKRKPLPSHTQMEQHPDSVGGALPTPAQTPEPIKNPSVDGSHEDDSIDEESPANKMTQLQSRMVLLQRQNTELTDALAKIVGLELEEGDLKSEDVLRAFRQIRLSRTPSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9C5</accession>
    <name>A0A9W8Y9C5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dienelactone hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9C5"/>
    <dbReference type="OrthoDB" id="2147163at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47668:SF1">
        <property type="entry name" value="DIENELACTONE HYDROLASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47668">
        <property type="entry name" value="DIENELACTONE HYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G01940)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dienelactone hydrolase" evidence="1">
        <location>
            <begin position="35"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="251" mass="27913" checksum="59FD01C1CFD1555C" modified="2023-11-08" version="1">MSTESAQSQACCNTPAVVSKGYSPKGDYIQVDGLKTYATGPKDAKQGILVVYDIFGFFNQTLQGADILAHTDKDHPYQVFIPDFFEGEPADISWYPPDNEEKGKKLGEFFKTKAAPPKTLPRIPKIVDELSKSKGIEQWAIVGYCWGGKIVNLSSKEGTKFKAAAACHPAMVAADDAPGVTIPYIMLPSGDEPKEDVEAWEKGLNVPHVVEWFPDQVHGWMAARGDLEQEKVKKEYERGYKLVLDFFHKHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9J1</accession>
    <name>A0A9W8Y9J1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003799</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9J1"/>
    <dbReference type="OrthoDB" id="62952at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF10">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="2">
        <location>
            <begin position="325"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1102" mass="124872" checksum="0A7B19305937BE76" modified="2023-11-08" version="1">MYNILFGANFQLTLQADGISTGSTITGDAGAQVRTDQSSLHPNHLSFSVNRSSLDHSTVDSIVSSDLWSAAYREAVESLGKGVDVAILVGSSAAQLFVELEGIDKGTTQQSAFLRGVAYLRSIQVPLERFKLALDLASPLGNLDPAASTVLGVVRSVTAIAISFATADLEFARQIGEMLEQISYIDDCDTLGQRVNKTDIHKALVCVYQKILEFYRVAHEILTQRGRKLAMKMVLETDRLPNIIKDFLGHADILRKLIEKSTWEIVEDIKSMLYDREIARWLESSRMNQQARYHNRLRELRSDEACKFLLTHPDFTHWCNASNSQQLLVLGEMGCGKTVAMTFLVDELNQRNGCQIPRPKVCYYYCRDDESGQAVSILSGLILALLEQLSGLKKMFYEWYKENQASGTLEPATSASKLGEFMELVLATFDRMLFIVIDGLDECDRASRRTLLRVLETLLKKTPRLKILLSSRPEEEILEQLNQTTRITLESNVDRDSVIVRHTVQEQLSYLSKDVRALVIETLSRLAQGSAIWTKMTVELIEVRKIKALAPMRLFLQDMSLPGQLSELYTTLLSRNSSNDPENEELAALALKLLAAACRPLSIQELTWAVALAAAQHEVKTVAALAQLVDHQRVMSLIYPFITRVDFADLRKRQVRLVHQSVKEFVVQDWSYLKGFDTSTASSQAIAPRIESLEAFMLDICIEYLLLQEVGTVPLFSEEQIAIDELPQEVDLFSDRKSFEYNVECTWEVWEEDMICYDPTERGFGEFFVYASSHWLKHFGATERGSFPRIAKLERLCQAGSMRLHNWINQNCRPGCAIKARFDFDSRLYDPLSITSLYGSDAILRDMLQNSDFDKDKYLRSPAASAADQILQWGDLSKLKILFLEGKIRYQLRNLEFFRLVIKRWSDFGSRHENWAEVFDLVDDVLDVLVNEQWGNELLCIAARAGCLPMIQRLLNRAHHMEELRTELWRDSRPIAEAILGNHADAVECLLSETDLEVDLQSLNSRGESLLHMASVHCNPAIFRLLVPLVQRVIHQVDSHGETTLMRIVRSDSNSKGRYEAARILLSYVEADRTTNIVNGQQEALQLAVQLGDAEMCRLLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9J2</accession>
    <name>A0A9W8Y9J2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9J2"/>
    <dbReference type="OrthoDB" id="10050400at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031942">
        <property type="term" value="C:i-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24145">
        <property type="entry name" value="Mgr3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002665">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040201">
        <property type="entry name" value="Mrg3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28142">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE I-AAA PROTEASE SUPERCOMPLEX SUBUNIT MGR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28142:SF1">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE I-AAA PROTEASE SUPERCOMPLEX SUBUNIT MGR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="432" mass="48770" checksum="F09AFD65C73E2291" modified="2023-11-08" version="1">MMFSRTISRAAPGISAFARCEAQCSVHSFTKIPIRTWKPIPQPLGRRYRSTIREQFREQYHKSPVLFPFAVGVIVLVSGICVLYIPWYYKNVIIKPYHNFPEPVAKKLRKALFYSRGKWLDIREANKYFRQALALADEMGMDPFSDEIMGVKYTIAALFEDAGYYSLAIDVLEIMRNDCQRWVDEFSDRHWNNGNRSRVKKNLVQILLKLGQLYDTRYVNEPDNGEKRLVEAVESALSEKQRRERDGLKTGEGPWLTNDELGGILEALGTHYEQFDSHYLATPLFVKALELCAPTSCHSVVLMNNISTCLAQQSPPSSSNALTLSSAPSDFPITSTAPRAILIDQARQWATKALAQASTIQPPNRNEECDVACAVATHNLGEFFEMEGRVKEARLKYEEAASLAKAVGFAEGTTNARAGLKRLKEMEKKKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9M9</accession>
    <name>A0A9W8Y9M9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Stress-response A/B barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006244</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9M9"/>
    <dbReference type="OrthoDB" id="1601230at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013097">
        <property type="entry name" value="Dabb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044662">
        <property type="entry name" value="HS1/DABB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33178">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33178:SF10">
        <property type="entry name" value="STRESS-RESPONSE A_B BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07876">
        <property type="entry name" value="Dabb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00886">
        <property type="entry name" value="Dabb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51502">
        <property type="entry name" value="S_R_A_B_BARREL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Stress-response A/B barrel" evidence="2">
        <location>
            <begin position="3"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="110" mass="12366" checksum="EF858F73EB61E635" modified="2023-11-08" version="1">MTIVHVVLFEWKPELTEEKIQEACKGMLALKEQCIRPTSQKPYILSSSGGKNNSPEGHSGGLNHGFVVEFASAEDRDYYVNKDPAHQDFIKFVLPLIQGARIVDYEPGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9S6</accession>
    <name>A0A9W8Y9S6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7923 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_004766</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9S6"/>
    <dbReference type="OrthoDB" id="2270193at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057683">
        <property type="entry name" value="DUF7923"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37543">
        <property type="entry name" value="CCCH ZINC FINGER DNA BINDING PROTEIN (AFU_ORTHOLOGUE AFUA_5G12760)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37543:SF1">
        <property type="entry name" value="CCCH ZINC FINGER DNA BINDING PROTEIN (AFU_ORTHOLOGUE AFUA_5G12760)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25540">
        <property type="entry name" value="DUF7923"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7923" evidence="3">
        <location>
            <begin position="76"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7923" evidence="3">
        <location>
            <begin position="134"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="237"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="25"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="278"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4371547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="300" mass="32913" checksum="3E6FAEFF1B8DD694" modified="2023-11-08" version="1">MDHEVDLLGFDDGFSAVRMSGASALQDLEAAAESCRRVMKQSQEEYDELLAKYDALRSRRDGGEVSQGAATEERAADAYVVVLIDAHSHKFKTNLMHGAESGGSHTAKLLKKAASDLQSYIPIEIPTVSSPNFPRALGGFAAGFSREEVFFDFVDVVDEETVERKITGLFQLYVKDPQCKQVLLGASGSMSYLRVLEAHQVFAKKITLIQRGVEDTSLMRLGLNSVTFPQVFESLSDGNSTKRTSSGNRSFDSNVSHWIEEPEEGTTEETTGPEDFTNHGQSSKESVTEWTTPTGVLIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9T9</accession>
    <name>A0A9W8Y9T9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Lysosomal dipeptide transporter MFSD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Major facilitator superfamily domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">N0V83_006335</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Lysosomal dipeptide uniporter that selectively exports lysine, arginine or histidine-containing dipeptides with a net positive charge from the lysosome lumen into the cytosol. Could play a role in a specific type of protein O-glycosylation indirectly regulating macrophages migration and tissue invasion. Also essential for liver homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-alanyl-L-lysine(out) = L-alanyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79415"/>
            <dbReference type="ChEBI" id="CHEBI:192470"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-alpha-aminoacyl-L-arginine(out) = L-alpha-aminoacyl-L-arginine(in)</text>
            <dbReference type="Rhea" id="RHEA:79367"/>
            <dbReference type="ChEBI" id="CHEBI:229968"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-alpha-aminoacyl-L-histidine(out) = L-alpha-aminoacyl-L-histidine(in)</text>
            <dbReference type="Rhea" id="RHEA:79375"/>
            <dbReference type="ChEBI" id="CHEBI:229967"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-alpha-aminoacyl-L-lysine(out) = L-alpha-aminoacyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79383"/>
            <dbReference type="ChEBI" id="CHEBI:229966"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-arginyl-L-alpha-amino acid(out) = L-arginyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79371"/>
            <dbReference type="ChEBI" id="CHEBI:84315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-arginyl-glycine(out) = L-arginyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79391"/>
            <dbReference type="ChEBI" id="CHEBI:229955"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-aspartyl-L-lysine(out) = L-aspartyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79411"/>
            <dbReference type="ChEBI" id="CHEBI:229953"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-histidyl-L-alpha-amino acid(out) = L-histidyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79379"/>
            <dbReference type="ChEBI" id="CHEBI:229964"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-histidyl-glycine(out) = L-histidyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79395"/>
            <dbReference type="ChEBI" id="CHEBI:229957"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-lysyl-L-alanine(out) = L-lysyl-L-alanine(in)</text>
            <dbReference type="Rhea" id="RHEA:79399"/>
            <dbReference type="ChEBI" id="CHEBI:229954"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-lysyl-L-alpha-amino acid(out) = L-lysyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79387"/>
            <dbReference type="ChEBI" id="CHEBI:229965"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-lysyl-L-lysine(out) = L-lysyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79403"/>
            <dbReference type="ChEBI" id="CHEBI:229956"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-lysyl-glycine(out) = L-lysyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79407"/>
            <dbReference type="ChEBI" id="CHEBI:191202"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homodimer. Interacts with lysosomal protein GLMP (via lumenal domain); the interaction starts while both proteins are still in the endoplasmic reticulum and is required for stabilization of MFSD1 in lysosomes but has no direct effect on its targeting to lysosomes or transporter activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9T9"/>
    <dbReference type="OrthoDB" id="424834at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052187">
        <property type="entry name" value="MFSD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512:SF12">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G00260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="291"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00044876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00046376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ4369250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="412" mass="45312" checksum="F74AABBA2336D3CA" modified="2023-11-08" version="1">MKRPRAMLWGNAIYTVGAIFIAAATTVRSYKFMIFGIVVQAFGDIATQVAQYKVFSSWFAPANGFASTLGFELGIGKIGSFVGKSTANVIAKNTGDFSWVYWTAVFMNLFTNVVTLVFWFFTRWCGKKYSGTTDPATGEKLTENNKTFEIRKVLRLPWTFWGIVGFSLFQTSAAVVYSANATEMAEQRFNIDAVTAGWYSSMAQYLGFFLVPIIGVIVDLFGHRLTLLLVCGVGMLLSMCLAAWGPTISGTAASFGIYAVASTFGPTVIIDSIRTSMWYQEVFGSGYAIKIAVNNSMNIIIRIITGVIQDHDNDSYDNVVIVYVILTAGSVVVAAAMFVSGFWNPDVGHLQWTRKRRIAKGNVINDQKEKFEAGEKGARNRKLSMTLFVALLFLIAGAWAAYFWGVATGNND</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA35</accession>
    <name>A0A9W8YA35_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_003775</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA35"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072:SF2">
        <property type="entry name" value="AMIDASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072">
        <property type="entry name" value="AMIDASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="5">
        <location>
            <begin position="86"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="3">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="219"/>
            <end position="222"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4372002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="545" mass="60433" checksum="A2B3FB44673AAA48" modified="2023-11-08" version="1">MTVSDTPPWLQTVTKKRQIRDDAIQAFIEMHKAPSESESLHLDEPGTTRNTLKDALAIDNIEEISQAIRSGVVTASQLLEAYIYRLTEVLFNDAIKQANELDDYFKKTGNLVGPLHGVPMTLKDQFDVQGYDSTLGYVGRAFKPATRDCALVSMLKQNGAIIVAKSNLPQSIMWCETDNPLWGLTTHPRNPAFTPGGSTGGEGTLLSLRGTVIGWGTDIGGSVRIPSHMNGLYGLKPSSTRLPYRGVSVSTEGQEHVPSVIGPMSRNIDSLVAVTKAVIDQNPWDRDPKCCPVPWHSDLFQQARSKPLVIAVMRDDGVVRPHPPITRVLDEVAAKLEEAGHEIVTWTPGNLHQECIDIMDQYYTADGGEDIRRDIQVGGEPFIPHVEALVNKGKPISVYNYWQLNKQKLAAQERYLDLWNSTKSPGTGKNIDILLTPVMPHSAVPHRKCRWVGYTKIFNFVDYPAVILPAGHVSKELDRDAAQSMGEYKPRNALDEWNWNVFDLDSMDGMPIGVQVVARRLQEEKALGAAKAVDEILKDMTKVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAH8</accession>
    <name>A0A9W8YAH8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Laccase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_003732</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4371959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAH8"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13854">
        <property type="entry name" value="CuRO_1_MaLCC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13880">
        <property type="entry name" value="CuRO_2_MaLCC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000021">
        <property type="entry name" value="Extracellular dihydrogeodin oxidase/laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000038">
        <property type="entry name" value="Extracellular dihydrogeodin oxidase/laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF71">
        <property type="entry name" value="OXIDOREDUCTASE TPCJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040818408" description="Laccase" evidence="7">
        <location>
            <begin position="17"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="10">
        <location>
            <begin position="82"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="8">
        <location>
            <begin position="205"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="9">
        <location>
            <begin position="465"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4371959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="614" mass="67671" checksum="A33B347D8A70F8CC" modified="2023-11-08" version="1">MHSFLSLLSVAVGASALYVPSTPNEANGLVSRDYRDNSGLTPGCENSPTSRQCWGEYDINTDYYNTIFHTGVTREYWLSIDEIDCAPDGYLRKCMAANGTMPGPAITADWGDEVIVHVTNNLPSNGTAIHWHGIRQLNNNQYDGVPGITQCPIVPGSSMTYKFHATQYGTAMYHSHFSLQAGMGVFGPLVINGPATADYDIDVGTIFFTDWDRSTAFEHWATVATFNVTFNGLGTGLVNGMNIGNCTLTNTTNPDPNCIGEGKKYELVLEPGKKHRLRLINMAIESWFQFSIDGHKATVIGADLVPVVPYETDSVLVNMGQRYDVIIEANQEPKDYWLRGGFETNCIPNGNADHINGIVRYSNESKAEPSSVSTVVQQWNCLDEPAEATVPWLPVDVTNLDGGVNYQNVTGEYWTGKFLRWSFNKADGGFMWTNWSQPALGSVVAGDISSIPVQDNIFQVGNNVSSFDATKNETGKTEWVVLVIDEKTPIVGIAHPMHLHGHDMMLLASASNVSWDGTTDNWQWKNPTRRDTVSLPPNGYMAIAWALDNPGLWFLHCHIAWHSSQGFGVTMLESAHLLAEGEATSDWKPKAEPQCAAWNEWYPTSPWPQEDAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAI3</accession>
    <name>A0A9W8YAI3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Class II myosin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">MYO1_3</name>
        <name evidence="1" type="ORF">N0V83_004280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAI3"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4372506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="73" mass="8520" checksum="8BF07A728F7FE909" modified="2023-11-08" version="1">MRQKSQFAKKAVFESTKKKEVGVSDLTLISKISNEAINDNLKKRFENGEIYVRRHARGSPTPPRYTEIYWNCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAJ1</accession>
    <name>A0A9W8YAJ1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">rRNA-processing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PWP1</name>
        <name evidence="6" type="ORF">N0V83_004132</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAJ1"/>
    <dbReference type="OrthoDB" id="270624at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000457">
        <property type="entry name" value="rRNA processing protein Pwp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001345">
        <property type="entry name" value="WD40 repeat-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044285">
        <property type="entry name" value="PWP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14091">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14091:SF0">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="256"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="392"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="494"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="504"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="526"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4372358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="535" mass="59423" checksum="71514A3DF0700CE5" modified="2023-11-08" version="1">MSMITATTWVPRGFAAPFPSRYQFDEDEYERISKLANLQLEDAKEDLEEAQAEAKSKSNGTSGKSSKAKDDDDADDDLKEYDLEHYDDEVAQDQGDTMAMFGNVKSLVFHENDEDDPYITMQGGEEEDDEEREELQILATDNLVLAGRIEDEVAHLEVYVYEDEDDNLYVHHDIMLPAIPLAVEWLDLPVGKSVGTSDGKGNFVAIGTMDPDIEIWNLDVVDSMYPDAVLGQGAEDAANADKAKKKKKKKSKKVNDEFHVDSVLALAANRHHRNLLASSSADKTIKLWDLNTTKCAKSYTYHTDKVCSVAWHPVESTVLLSGSYDRTVVAADMRAPDAKAPRWGVESDVETVRWNPHDPHYFYVSTENGMIHYHDTRNAPKDPSASKPVWVLQAHDESISSFDINPVIPGYLATGSTDKQVKLWNIQENGPSMVVSRDLGVGRVFSTTFAPDKEVGFRLAVAGSKGAVQIWDTSTNAAVRAAFANKVTLPQMKQDGKERLVGLDEPETDSDTEEGESDDGDEDNGEEKGWESMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAL1</accession>
    <name>A0A9W8YAL1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Thioredoxin-disulfide reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TRR1</name>
        <name evidence="9" type="ORF">N0V83_003923</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAL1"/>
    <dbReference type="OrthoDB" id="371245at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016668">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097237">
        <property type="term" value="P:cellular response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="8">
        <location>
            <begin position="55"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4372150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="327" mass="35736" checksum="CEFFD0CD91BD5EEA" modified="2023-11-08" version="1">MYEGFLALGIAAGGQLTTTDEVENFPGFSKIQGGKLMEQMREQSEACGTEIISQTVAKVDLKARPFKYWLHPMGDDEEIEEEEHTADSLIIATGAKARRLDLPGESKYWGNGVSACAVCDGSLPMFRDQPLVVIGGGDSAVEEALYLTKKAKKVTVLVRRDKLRASRTNARRLSTHPKIQILYNTSASEIKGEDKKNGLMTQLVVKNNVTKEQQTLDARGLFYAVGHDPATELFKGQLDMDEDGYLITKPGQGLTSVEGVFAAGDVQDKRYRQAVTSAGSGCVAALEAEKWLAEQDDDVANELETENQAEKSQTNGVVPEYRSNPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB48</accession>
    <name>A0A9W8YB48_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB48"/>
    <dbReference type="OrthoDB" id="4158258at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="160" mass="17295" checksum="3C2B0B509044700E" modified="2023-11-08" version="1">MAEQASNSDKREGENVPAYDDAEHASAPPAYTAVAAGMQSLGIGDPNETISVPMVVSTEQKRKRDLFLPMGKAGKITQTVIVRKMKRDHYLKHYAKDAEGNYIGTEKAAVDAGLVFVPGKSSNEDILEQIRKVAFGKQHYNSDFAHGYGMFQTGVGGGAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB64</accession>
    <name>A0A9W8YB64_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">TBP-associated factor 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">taf2</name>
        <name evidence="12" type="ORF">N0V83_004239</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="308861at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09839">
        <property type="entry name" value="M1_like_TAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000011">
        <property type="entry name" value="Transcription initiation factor TFIID subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057345">
        <property type="entry name" value="Ig-like_TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037813">
        <property type="entry name" value="TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057991">
        <property type="entry name" value="TPR_TAF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25316">
        <property type="entry name" value="TAF2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25577">
        <property type="entry name" value="TPR_TAF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 Ig-like" evidence="10">
        <location>
            <begin position="580"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 TPR repeats" evidence="11">
        <location>
            <begin position="768"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1221"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1260"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1359"/>
            <end position="1508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1223"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1369"/>
            <end position="1381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1382"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1404"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1443"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1459"/>
            <end position="1470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1479"/>
            <end position="1489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4372465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1508" mass="167393" checksum="F56916DC5766567D" modified="2023-11-08" version="1">MAPLEMAPAPDDSSTGDSFTLIKQQVELDVSFAPPRVKGRTTLDIQPDKPQLQVVILNSRQLKPTSIKIEGEKAAFRYSNLHQRLTLYPGTGLEQYHFVKERIGRHVVDREDELVIYVPQKVKVREVKPSEAGVADAAQGTFYASMKLEIEYELNDFRDALQFVGVEDGDARFPHAYTRNSPFPGASSCLFPCIDDGETRCFFDVSIRYPRTLGDALSKAPRPNATGQSLTNRAEKADSVMLDADDDEVQLSVEEKAMEMSVVCSGTLTDDVRTSKPLKPSGANTLQISDVADTSRKTASFTCDTPVLPQHIGFVIGPFEEVDLSEFRDLADDERLGENAIKVHAFCLPGKEDEVRNSAMMLARALDSFTERFQSYAFGNSYKLAFVDDLEGDTTHTASFSICSSRLLFPETVWEPMEHTTRVLVHAVASQWIGVDVVAHNPEDYWIIVGGSWFMAELYLRELFGRNDWRFRQKLMVDKVIQMDRKRPSLQQLGEFVQLDPSEQEFMEIKSLMVLSILHNRLIKQSGKSGVDRILYRMLFNARQGKYSNGAIATDDFLDLCEKVGHQKLEAFFSQWVVGSGCPTFECFPSFNKKKQVVQLQIRQTQADSTVTTIEPPLAADDFMREAKEKARDFRPPKESSTFVGPMTIRIHEADGTPYEHIVDINSPNVKVEIPYNTKYKRLKRNRLNKERMAAAVGLDTSGETQEDVTFYMLGDLFTTPEEHDEWRLDDWPAEDEAKQEQEAYEWIRVDADFEWICKANINDMPSYMYVSQLQQDKDVVAQAESIQFLANKEGHKLISTFLVKTLMDSRYFHGIRTMAAEVMAQSALKRTEWIGLFHLIKAFRELFCLPGSPMTRSNDFSDRSLYLIQCAIPKAIARIKGEDGKAPMEAKRFLLDVVRYNDNRGNAYSDDHYISTLMRCLAECLTSARTNLHHDLNHQEWAEERDFTDKAMGELTRHQRLDEWIPTFQNIYTTTALDCAMKLMINRVTPFKPTEFLQYAQLGNADNVRLKAWECIVRLGLFRKDTILRFMIHEIRSDPSPYFRKQLLRIFGLAVGQVATGDIYTPEKAAETAVDSLVVENEISNSDRASALARQNLPGALKGLKIDLQNNQVLRLALEEALKSTTLSTSNMSELLDFCDMVFDDQKRNMLPVKLRLPRYWKVEHLGKGELRFWQTDAVRTKPLRPLFTAAPTQLAREVPVQQPPPPPVPLPKLSLKIGANKSKPAASPAATSPPLTQLTGSAVPKSRAQSLVFSPTIHQSPSPVLHSTKASPAPSPVVVKTESRASTPEVPLMQQPKKKSATPAPGVVHQTPVPPAASATVSARPTPAPLNVPAFVATPPAIASPIPLPAPKLKIRTKTANGSAAPSPRPSAAPSPRPSVAPSASSSRPSAPPPASATPKDKKSKAKSKIVKLRLAPSLLARFPSSEGSSKKRKLLGVGAGEERPFKRQSLEPNGVLNGGSGGSGGKSGLVTVEKSAGGGGGGGGGGDRPRLLLKMKVGRGALPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBY4</accession>
    <name>A0A9W8YBY4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoglycerate mutase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003578</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histidine acid phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBY4"/>
    <dbReference type="OrthoDB" id="10257284at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050645">
        <property type="entry name" value="Histidine_acid_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567:SF110">
        <property type="entry name" value="2-PHOSPHOXYLOSE PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567">
        <property type="entry name" value="ACID PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="488" mass="54100" checksum="73CD159D16AACCCF" modified="2023-11-08" version="1">MTTLIPRQFYSQEELDKLYPKELELQLVQITGLQPYWPYCNAARQLRSVILNTKDFSQWDQLKYRRRIETFGMNDGPVIAAGPRGEFDAVCQPGELTDKGRETTLALGQRLRHLYVDQLGFMPKLISSSDMIYLRATPMPRALESLQQAFSGFYPPSARTADFPPPTILTRTPADETLYPNDASCRRFAKLSRAFAQRTAEHWNTTEDMEYLSKLFSKWMPGEAKVAVDSHPRLSGIMDTVNATDAHGPETKLPKEFYDPKARAIIDKVAVEEWFKGYTESAEYRMLGIGGLMGDVTSRMAGSVEHNGNDGLVEIGGVDGKLGKGRGGETDIKFALSGCHDTTLAGVLTSLGAFDGEKWPPFTSHIAFELFRSKRGGAGAGGADAGALRVPKTSTTTPTLQQEVGSKSQSWWSSLFGSVKTSKDEAPATPEGIARRPITELSQAQRDELQGYYVRIRYNDKIMQVPGCKVEGKHLEGDTTFCTLVRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBZ6</accession>
    <name>A0A9W8YBZ6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_003515</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBZ6"/>
    <dbReference type="OrthoDB" id="4494341at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000293">
        <property type="term" value="F:ferric-chelate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF12">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_1G14340)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="313"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="11">
        <location>
            <begin position="333"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4373222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="619" mass="70057" checksum="AF5E4CEB1A3CEF29" modified="2023-11-08" version="1">MSTFLRPRRVEHPTTAITDSLARRHFLEGWDAASFDSLKYSYGLTGVDQVGNYLWVDMFLYMLVAIAVILLGLRIANMWWRHNRHLSTMGNPSRQAYWATNRTSWWPWLNRHVLAAPLWKKKHNAQFQISSAIDNGTLPGRWHTIMLVVYVGLNVAWCLALPYKVLDHRETIAALRGRSGSLAALNLIPTILFALRNNPLITLLQVSYDDFNLFHRWAARITIVESLVHTIAWMYNTTSGGGWAAVATGLRTEGSYGWGMVGTVCFTFIGIQAWSPFRHAFYETFLNIHRLSVVAALIGLYKHLEMHHLPQMPWMHLIFLFWGAEWVFRGLSILYYGFSLKKRSSIKVEAMPGEAVRLTIDLVREWTPRPGCHVHMWMPALALWSSHPFSVAWAASPTPDSKEMTLPILEGDALNVPGAPQKSKQISLICRARTGLTRKMYERACKSPNESFTTWGFIEGPYGGHHSLDSYGTVVLFAGGVGITHQVMYLKHLVGGFNLSKTSTQKIVLIWTVPTSDCLEWVRPWMDEVLRMKGRKQCLRIKLFISRPKGRVESSSETVKMYSGRPQPSALIEEEVRNRVGAMAVTVCASGGMADGVRAAVRPWLTEGSVDFIEEAFTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCC9</accession>
    <name>A0A9W8YCC9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">COQ4</name>
        <name evidence="9" type="ORF">N0V83_002538</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Lyase that catalyzes the C1-decarboxylation of 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoic acid into 2-methoxy-6-(all-trans-polyprenyl)phenol during ubiquinone biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoate + H(+) = a 2-methoxy-6-(all-trans-polyprenyl)phenol + CO2</text>
            <dbReference type="Rhea" id="RHEA:81179"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="Rhea" id="RHEA-COMP:10931"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="ChEBI" id="CHEBI:84443"/>
            <dbReference type="EC" id="4.1.1.130"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion inner membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the COQ4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCC9"/>
    <dbReference type="OrthoDB" id="4249at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120539">
        <property type="term" value="F:4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03111">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007715">
        <property type="entry name" value="Coq4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027540">
        <property type="entry name" value="Coq4_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922:SF7">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ4 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05019">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0830">Ubiquinone</keyword>
    <keyword evidence="1 7" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00081568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4373799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="289" mass="33045" checksum="8634F6EB45961B65" modified="2023-11-08" version="1">MAKPARVSQLSKLVSRPGSPCASFQQPLRCFSVLNRPSPKYEGHIPLTRVERLGLAVGSGLGSFLDPRRGDLIASFGEATAQPYFIYKLRDRMLLNPTGRRILRDRPRLTSKSLNISRLRQMPQNTLGYAYAFWLDREGVTPDTRAQVKYIDDEECAYVMQRYRECHDFYHALVGLPVFREGEVALKAFEFANTGLPMTGLAVFSALTLKKAEWRRFWDIYGPWAVRNGARSDDVINVYWEEELETDVGELRTRLGIEKPPDLREMRKVQREARKKAREAGESMARAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCQ2</accession>
    <name>A0A9W8YCQ2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002718</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCQ2"/>
    <dbReference type="OrthoDB" id="9997739at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843:SF2">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="15"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="307" mass="34950" checksum="77EF2B419E366111" modified="2023-11-08" version="1">MASSRSARKAFIQSDLFTFYIGEEKKPFIVHSKAVAATSEHFRAMINGDMVEAKTRSAEILDMEPSDFARFLEYAYRRNYTIPSWKLDETIPGQPKNDTPAVVPSPPPPEEVIEHVPPNELPSPPPEPEPPVPNDPSTIPSTLRPLKKSTKSKRNKMAFRLAFQSQKFFLSGPDSTMIENFRPEGNSAPEQDFVPVFLAHARLYTFAEMRLVYPLRNLVLHKLYKTLMCFQLYNHRVGDIVELARYAYDHGADRSKDGTLDALRQMVVDYIACEVDTIGKHADFIRLMEDGGEFVSDFWNIVSRFTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCS0</accession>
    <name>A0A9W8YCS0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_002748</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCS0"/>
    <dbReference type="OrthoDB" id="687730at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22679">
        <property type="entry name" value="FHA_SLMAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051176">
        <property type="entry name" value="Cent_Immune-Sig_Mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 170 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715:SF46">
        <property type="entry name" value="TO VACUOLE TARGETING VPS64, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G02420)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="705"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="3">
        <location>
            <begin position="181"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="500"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="602"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="500"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="602"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="640"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="683"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4374009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="737" mass="80012" checksum="0FEB852BE0A3BE2A" modified="2023-11-08" version="1">MTAVAPPVNFQNINRQAWSAEGVPNGGAMFMPRKSAQRTNSSSSLSSQASSTSTLSASSSQANGVAPPNGSEASAWAARKAKAPKGLWPASKTEPVSGITTARPQPVASTGSGSSAASAMSALQAPPAMVPSQHNGAVQSQQQNGVPRAQGQESTAVLHLVPMNGTFERKTITVPFFPDVLRIGRQTNNKTIPTPLNGYFDSKVLSRQHAEVWADRNGKIWIRDVKSSNGTFVNGARLSQENRDSDPHELREQDMLELGIDIVSEDQKTIVHHKVAARVEHAGIYARTSSEMIDLNFGELEGPQLSNMMGPGNQQQLANMRGRTPSQGSMNGQRLQGPSNVATYGNGMQQARHPNFWLQPITMEQVVKKLNAEIKAAKQQSQDLQQTHQYINAILSADPKKEPTKASPINQIKISPIKDISLKARFSDPPAPPPQQPLPEKPDAASSIRRSDTERPKTASPVRSDAQMSTLTEALTSTKKELESQNLRLKELEALLNEERRAREDAEERANRLERESSKEIAEHDMPLANGDIHKNEIVETVGEEASGLENGSASPSAADDTTARLQQRLELMVSEMNEMKQQMERYRVRAETAEADRKSLAEMIENIRKDNAKAASKGSRRRSRSGSQSARGAGGASVDELEDDNDTEEGEITIIREKDLEDDGSDGLVRRGGAQNGHPVEQNGTKQSENASNALVTRRPDRDLMYHGGPVGAIVTVVAIGVAVMAMLNQYPKAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDD4</accession>
    <name>A0A9W8YDD4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDD4"/>
    <dbReference type="OrthoDB" id="3801344at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="598" mass="68424" checksum="33D254CB0D8038EF" modified="2023-11-08" version="1">MAFPLYQRWKMNKRRASTLYTTSYGWKCARTVSYPGTFEELIEAEAAELRDNPGVFLELKNPQACLDGIPEELLLEICEHLWVPAVPDAFSSLAMLGRTNKRCKRVADEFLYKLTRDDTGQIEALDENPKLAGLVDTLQLYSLDYEMEQDKEHIRQKRHMYTRVLQSMVNIKHFDIMFNDPKLPNTSADHPAWMLGILNVLYKFERDIINSNASNQFMRLEVVTLDGIEILMVEDISPIFRLPSLKTLTLNGFLGPDHMSRRTWDIPDSSSSIQHLRLPWCFIGSEVVLQMLSAIKALKEFDFHYTTEYWRPLSPDTDPACEWAELSFTEMGDAIGKHKESLEELTILAKDDGEMEDMVYPEGWNAGKIGSLRDFSKLKSLSSHLRMFLYLNEAKDDINKYLPAGLERLRMHCPEWDVVEESDGDEEPDDDGEPDDDEEEDEPGEEFDEHGEEYDGYGEEYDGYGGEYDGHGEEFYGDGQEFDGHGQEFDGHGEQFYGHGEQFHGPGQQFYGPGQQFYGSGEQFYGSGEQFHGHGEQFYGLGEQFEYGDAYKGELGEAFKAFGDDIGEQKEVAYKIDGGKGYAREKPEYVLDGGHLWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDD7</accession>
    <name>A0A9W8YDD7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002807</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDD7"/>
    <dbReference type="OrthoDB" id="9977870at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="345" mass="39849" checksum="4803A481EE9E5E27" modified="2023-11-08" version="1">MEQVMTPSQVDLILSHALQSPDNGSLRDSGYCEDGMSAIDLPKPVVNASPPVTTPLAAGTPVHRSRHEAINIDDALANEAFKSFKAQQKEQFEKVAAFECNQRKALSAHHQSSLDGRVAQHNRNKDERKEQVGLSRDTALCKLTQTPQHVADLEHLEELQISAEHDLRKAQIQETQNVATALKYMEAYCLGTNVNHPEHAHTVTEEDFRKLDRQRQVQQNLPRKHESAINVLRAKQERETKRRFQKHEAELNVMDGDHKDEMAKRTVEFKKEQDKLETLIEVRRRRLLQRWDLKFEMWRRDWEEQHGTTLKTKLEHESWPLQRMETVTPIPESSSLARFVTKVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE29</accession>
    <name>A0A9W8YE29_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Flap endonuclease 1</fullName>
            <shortName evidence="15">FEN-1</shortName>
            <ecNumber evidence="15">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">FEN1</name>
        <name evidence="19" type="ORF">N0V83_002110</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Nucleus</location>
            <location evidence="15">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Nucleus</location>
            <location evidence="15">Nucleoplasm</location>
        </subcellularLocation>
        <text evidence="15">Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14 15">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="15"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE29"/>
    <dbReference type="OrthoDB" id="1937206at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09907">
        <property type="entry name" value="H3TH_FEN1-Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09867">
        <property type="entry name" value="PIN_FEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000009">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000003">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF9">
        <property type="entry name" value="FLAP ENDONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00842">
        <property type="entry name" value="XPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6 15" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9 15" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 15" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 15" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="18">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="17">
        <location>
            <begin position="110"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="289"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="292"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="315"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="348"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ4375031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="359" mass="40384" checksum="F224CD199F02F0E9" modified="2023-11-08" version="1">MSIYSFLIAVRSDGQQLMSDTGETTSHLMGLFYRTLRMVDNGIKPLYVFDGAPPKLKSGELAKRFQRKSEAHAAAEEAKETGTAEDVEKFSRRTVRVTREHNEECQRLLKLMGIPYIIAPTEAEAQCAVLARGGKVYAAASEDMDTLTFDSPILLRHLTFSEQRKEPILEIHLDKVLEGLGMERKQFIDLCILLGCDYLDPIKGIGPSTALKLIREHTDLEGVVAHIKSNPKGKLTIPDDWPFADARLLFLEPDVCPADAPECDFKWEAPDVEGLVKFLVEEKHFNEDRVRNGSSKLQKNMKTAQQSRLEGFFKPVEKTSEEKASLKRKADEKLEAKKKKQKEDAKAKKQAKAKPRNAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEB1</accession>
    <name>A0A9W8YEB1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_002772</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEB1"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03048">
        <property type="entry name" value="GST_N_Ure2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF8">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE GSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01151">
        <property type="entry name" value="Main.2:_Nu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="3">
        <location>
            <begin position="4"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="4">
        <location>
            <begin position="95"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4374033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="21997" checksum="0267C2EACAB482CF" modified="2023-11-08" version="1">MSSPPTIELLTAATPNGQKISIFLEELGLPYKTTPIDLGKDEQKTPSFLATNPNGRIPAIIDHSRTPSFPVFESGAIFLYLAEHYDTYKNFSFSDADERSEMLQWLFFQNAGVGPMQGQANHFYRYAPEKIPYGIKRYQNETKRLYSVLEARLQDRDYLVGKGKGKYSVADMSTFTWVRWAPWAGIELGEFPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEK8</accession>
    <name>A0A9W8YEK8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Yippee domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_001070</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the yippee family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEK8"/>
    <dbReference type="OrthoDB" id="6407410at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034751">
        <property type="entry name" value="Yippee"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004910">
        <property type="entry name" value="Yippee/Mis18/Cereblon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039058">
        <property type="entry name" value="Yippee_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13848">
        <property type="entry name" value="PROTEIN YIPPEE-LIKE CG15309-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03226">
        <property type="entry name" value="Yippee-Mis18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51792">
        <property type="entry name" value="YIPPEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Yippee" evidence="5">
        <location>
            <begin position="55"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="22"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="183"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="211"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4375793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="255" mass="27601" checksum="66B0EA1FD5A12FCF" modified="2023-11-08" version="1">MSNSPTTPFPLYLFPSIPFRRRKSSTASTSTSPSSSPSSSPPQASPSYLSNTPTSHLRCHKCLSDLIPTSSIISKGFTGRHGRAYLVAPPAPPPPSSIVSVSGFSAAMGWSKGELPNTLTHKPQPRQLVTGAHTVSDISCRMCGCVLGWKYVGAEEDSQKYKVGKFILETKRVVKGVGWEDGSGSRVVEENEDDHEEGLGRVARGGGKDGVEEDDDIQFDSQDEDECEDLFSGVWSPQLARRRRRRKGTAFGRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEL7</accession>
    <name>A0A9W8YEL7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8004 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003488</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEL7"/>
    <dbReference type="OrthoDB" id="5300331at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058317">
        <property type="entry name" value="DUF8004"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39601">
        <property type="entry name" value="CHORIOGENIN HMINOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39601:SF2">
        <property type="entry name" value="CHORIOGENIN HMINOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26013">
        <property type="entry name" value="DUF8004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8004" evidence="2">
        <location>
            <begin position="377"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="784"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="803"/>
            <end position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="872" mass="95308" checksum="64A35D0276057A49" modified="2023-11-08" version="1">MLGEKKKSSHRLSSLFSVGSSDSHDSKSTKASTAPSSESSGRLTKVRNRLSSATHLAPEYPPPPPPKGGRDVSLSNPTIQPVESIEPAASLEPLDPPPALEVPSRGTSPSRSGSRPGTRPGTPNLDAPSEGNLKKLRRKSRLFGGGSVSDGAIPDTQHGHNSPLAWIVGHKGKVPYNLTMLLNGEQVPELWDDAGDTFVYLFPRTSGKGPSFRIDSSVYAASQLLTRLVHGSLYSDHAAAPPNDLAERASRRVSSEAYGQSRTASPDQSQIGSSDGSKGSRALSDAVEDDYTEKHLYMPISLSSDAAVTPREPNLNSKDVDTLVTYRNFFAFLIGQSLVATERQSDIFDIFLRIADILSHYAFSNVDGTSFGEVAASSFDCYVDELTLADVRHSREKTIEAIVLGERMRSLSIYTEGFVHAAGKYDSIKEIGSPKFAMVSHKTATRLARAAMDLDNREATINGKLKDFEFPNVFAGIMNSAMASEGKIISFKKWRQSFMTTRSFVIDYYKAKYGSWLPKAKSKKNNLTTSGLNRLVLVDIYRDMSNLYDLLVDRKNLTNRTADGFLAEDEGDDIESVVHRALRKVLSEYDRSTPPVQPPVPFDLPLYPASLRPKSGDAKKDAKARSKKLHKDDVAKLLKQSRNPDAIQQTPFLEAFQQFEFKHASGGTLDDLWDLRSGQWLFLYAVIQSLPMLVVDAPGVRFTEGVEYFLCEVPRSGVPWGREDTTRTRTWFGVAGGAQVVSLPTDIVEHGVEGVFRRSHCWKKAQQWAENDTGLSAAMQELNASATPLPPPPGLLDPASADARSRSRSPDRRRESVMNLGLEALPLPPGVAPPLGSSPLLRPASSSDPSKTFDAILGTNDSSNSKDKKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFL5</accession>
    <name>A0A9W8YFL5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFL5"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="242" mass="25461" checksum="7929E81A67F38CD7" modified="2023-11-08" version="1">MHRRNSLRRGTTSNHESSLDELDGFRSNARSGYTSLASNAPTSDPSSSSTSRCGNALRRSSNPPASAMDSGPPSNSNSGLHDGVGPRPISTSQSRSNPQWSPRTLRSSIQIQASALEHSPPVTKQSQATQVSDSSGGSQQADEDEDEITPLLQRLHIALQAVTGMQQQLAQMGAADDAEAAAEALRMMQHMQQKFAQILSSVEVTAADGRTGGDVEVELLAQIEEGVSQLNLNVGAGPADDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFV4</accession>
    <name>A0A9W8YFV4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001784</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the asaB hydroxylase/desaturase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFV4"/>
    <dbReference type="OrthoDB" id="412788at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044053">
        <property type="entry name" value="AsaB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041278">
        <property type="entry name" value="CmcJ_NvfI_EfuI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34598">
        <property type="entry name" value="BLL6449 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34598:SF3">
        <property type="entry name" value="OXIDOREDUCTASE AN1597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4376500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="338" mass="39016" checksum="B4F9FDEF2790B48B" modified="2023-11-08" version="1">MSGRNKEARAVLGCWIRQLGTTHLPPSSGPLYLTFKDTMRKLRVVRYFHPKSDMHETPPRADGNDGSHKHHVATTLNYWDDPKDGSRPAPIYLLKGRVTNKRPHRAHKFLVTDISGDEDKYSLDGHGFQYCSYESHEKEFIDEQAIRSNYYEECEKLVKDVTGATRVWIFNHKVRRGPTQWHHLGFNNVANRGPVTRTHVDQSYAGAELRLRHEFPGEADELLKKRYQIINIWRPIETILKDPIAVADANTVPDADLVGADMVESDFSGESWVVRHNPTHRWCYKHQMTPQDVLFIKCFDSNTTVARRALHSAFEDPAYRDMASRQSIEVRCLVFYDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFV7</accession>
    <name>A0A9W8YFV7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">H/ACA ribonucleoprotein complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Nucleolar protein family A member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SNU13</name>
        <name evidence="12" type="ORF">N0V83_001335</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Common component of the spliceosome and rRNA processing machinery. In association with the spliceosomal U4/U6.U5 tri-snRNP particle, required for splicing of pre-mRNA. In association with box C/D snoRNPs, required for processing of pre-ribosomal RNA (rRNA) and site-specific 2'-O-methylation of substrate RNAs. Essential for the accumulation and stability of U4 snRNA, U6 snRNA, and box C/D snoRNAs.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
            <location evidence="1 10">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFV7"/>
    <dbReference type="OrthoDB" id="1924699at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000002">
        <property type="entry name" value="NHP2-like protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002415">
        <property type="entry name" value="H/ACA_rnp_Nhp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00883">
        <property type="entry name" value="NUCLEARHMG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="11">
        <location>
            <begin position="19"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4376054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="126" mass="13713" checksum="BF062F59103F859D" modified="2023-11-08" version="1">MAEDNSAAWPLADSALSQEILDLVQQATHYRQLKKGANEATKTLNRGISELIVLAADTSPLAILLHLPLLCEDKNVPYVYVPSKMALGRACGVARPVISASITTNEASDLMSQIRTLKDKVERLQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG72</accession>
    <name>A0A9W8YG72_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Nucleoporin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_001502</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="102511at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044840">
        <property type="entry name" value="Nup188"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041634">
        <property type="entry name" value="Nup188_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048883">
        <property type="entry name" value="Nup188_N-subdom_III"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431">
        <property type="entry name" value="NUCLEOPORIN NUP188 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18378">
        <property type="entry name" value="Nup188_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21093">
        <property type="entry name" value="Nup188_N-subdom_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21094">
        <property type="entry name" value="Nup188_SH3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup188 N-terminal subdomain III" evidence="10">
        <location>
            <begin position="708"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="domain" description="Nuclear pore protein Nup188 C-terminal" evidence="9">
        <location>
            <begin position="1486"/>
            <end position="1873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="57"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1095"/>
            <end position="1121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4376219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1885" mass="207930" checksum="4F64C1790B740618" modified="2023-11-08" version="1">MAPIQSPELDLAKCFTGEQQLVTWDIAYRALCDPETASKSEALRTFLTSSDNTTILSRPWKPFPDPSTQEKSKFDSRTAPISVTPSHNGQYNLDEIKEDSLWLSKEARISEYAALQLVVQEWQSRPTTQLLSGLTEEEALSVQEAAGIANLGASTFVPNSSILATPLASRSTQFDTQDQRRLRIINVYHSTCASIIRISQLLVSWGSASHLRSQTIYGNDYLTIGAGWIEELGQSISTAQNGTSSTGSGAGALDECLKAVQKRCENLEGGYSWTVADTTLEAASELWVTAQTTELLHILHLALVHADLVVKGFVPAATIEDWLTTFMMRSFFREFPTVTVRQEPLVPVIQLLTSLVSLTILKVDLIIDDLDSGNCKNWDPSSYILNTGIVENITTIFGEAKLLGASPATPPTFAWAILTWKLTNRASYEEQERERLLESSNGAQNSAPASSALEESALAVARLEAHELFDNKMPYEELANASSEVGVLAIINQLVEIAIPAFGTPIDQISRDRFRLIFLQLIRAALGSDIVGYSPDLIVATYTILAGNKTFRTWVDADVSRYTDPVVAFFLEDEYVLRPLLLNEARLRYPYEMTPFLKFCSALTRGDRTSQNGMPTFVDKLTNTNRLMQRLPEGFRDYALTREEENANWVELTADLPLFSSSTLSSFRGQRRLMMSAASTPVDGLMIIPAGTEGIIVDDSTQPYVAVWHHQHSTLTYLVQLLATYSVGSGKVECTTQQPASRESAIEIIGLFADLMHSSLQSSEGLCSSELLEALGVSVDSTSDTINIVLAVFEEELLRLGQEPGNEASLELLVNCTHFLEALVVVAPNRVWPWLARSRLLEGDGNGSLAAILIGTEMVLGRYDFLIGCIRLYKGLVKDAVDRSIARKAPNKALSRFHSSTPSESGTSEKIMSTILFTFGRTLAGIYESSLGWKYSSPEHRPEINIAICDVFTTILKLAYGVDDAPKLSAKLTKSIANIAEYITQLYLTKSDNKLPTNPILASLVIGAGPEDKTLLTSSAALWRQQTQATLTLSDILVRVAILINKPWTHLEQQLFKATPLLARLYATSDKWKSQVVLLLETLVRGAVRVIGPEDAEMGDSNAKQTTLKKNEQQEPPSLLGHLGPRTAKNFLSVLSVLDEPLRIVDIQTNVWNLLSAVVTCKQQWFSLYLLTGSTPRESLRTKPDTAPNSRSKALLTRALDSLSNLTLPEKSVDDHPWPLYIAMLEFVSSAQNNWSWAMGDLRQQKDLIQKLLTFLKWMIKQPKQLKLSAEILERSYQNKFASLACEVIAMYLHSSRQAGDITALKDVVPSLGYLEETALDLPAYNVSLHGYLKQNLERQYPAVSLANLKRTTLYPESFGRAFFYNVDFAQQLLGFDSKWSGPREGTGFAGEAEKANFNLGLVESQVLLLQGWKLLSLELSYVVSKDERIAKVLIGVIKKCMEANTQSNLPEALFGQLMIARADLAFALLKKLVEAKVQTSEARQLLTPVWNAVRASTPDFDNVFSTDAVHYHRSLLRITYLTLHFHLIEDSDQSEDASFRSSFRGTMPASHNSYTEPISTQLLEILSDAVAKGFRSLATQLHADPSSVSPSDFALITALLQRIIAIPEMAKWQQQAALLFANSNTIRYATSLFSWSDRLTLSHNGINDPIYGELSLLFLLSLSSIQALAETMAVEGILSQLNTANLMNYYRRPGGMGPFDTPARLYSIWTKGLLPLCLNLLLSVGPPIAAEISSFLNQFPEQLLRASHAINSNPTTSRLSSSSASATKITLSVVSEIHSLALIAAILENVRKQGPKLGIQANEVGVLEWDGENVKEDVESWLSRKGALRERIVACDERELEMAGRKRGGGEDALSELEEKVVNELEDAGRCLGVGNGNGNGKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJH8</accession>
    <name>A0A9W8YJH8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">L-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="15">1.1.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">N0V83_000483</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(S)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + pyruvate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:19909"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.1.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19910"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">In the C-terminal section; belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">In the N-terminal section; belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.3" evidence="15"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJH8"/>
    <dbReference type="OrthoDB" id="1925334at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004460">
        <property type="term" value="F:L-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02922">
        <property type="entry name" value="FCB2_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000062">
        <property type="entry name" value="Cytochrome b2, mitochondrial, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000012">
        <property type="entry name" value="Mitochondrial cytochrome b2, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037458">
        <property type="entry name" value="L-MDH/L-LDH_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF148">
        <property type="entry name" value="L-LACTATE DEHYDROGENASE (CYTOCHROME)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00557">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0288">FMN</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="17">
        <location>
            <begin position="10"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="18">
        <location>
            <begin position="113"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00066458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00068515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ4377655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="509" mass="56954" checksum="AAF85DCAEE602B81" modified="2023-11-08" version="1">MNHDSNDTQAVKLTGEEVAKHSNRDSCWVIVHGRAYDVTEFLPEHPGGPKIILKYAGKDATEAYEPIHPPDTLDKYLDKSKHLGEVDMNTVEQQEKEFDPEEEERQKRIERMPILEQCYNLMDFEAVARKVMKKTAWAYYSSGADDEITLRENHSAFHKIWFRPRVLVDVEKVDMTTTMLGTKVDMPFYVTATALGKLGHPEGEVLLTKGAKKHNVIQMIPTLASCSFDEICDAAEDGQCQWLQLYVNKDRAVTKRIVEHAEKRGCKGLFITVDAPQLGRREKDMRSKFDDVGSNVQSTGGDDVDRSQGAARAISSFIDPSLSWKDIPWFRSITKMPIILKGVQCVEDVIRAVEVGVDGVVLSNHGGRQLDFARSGIEVLAEVMPILRQRGWQDRLEVYIDGGVRRATDIIKAICLGAKGVGIGRPFLYAMSAYGLPGVDRAMQLLKDEMEMNMRLIGASSISDLNPSMLDTRGLSMHTAPVPHDTLGLNVYDPLLGPQEKVIKEKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJN5</accession>
    <name>A0A9W8YJN5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_000332</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJN5"/>
    <dbReference type="OrthoDB" id="3037908at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051711">
        <property type="entry name" value="Stress_Response_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47540">
        <property type="entry name" value="THIAMINE REPRESSIBLE GENES REGULATORY PROTEIN THI5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47540:SF2">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="505"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="8">
        <location>
            <begin position="276"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="577"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="581"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4377507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="679" mass="76379" checksum="D8AE870DD8CF71F4" modified="2023-11-08" version="1">MPAPTPRRITQTGNLQPYRENAAVDLQQTSAARVYPHPILSDSGEDSRPASSTPRMNTLPPTVSRFNEAESTQIISSRSSPGLEVAGQYADPTSGHSFLHRAWRRISNNDSSLILRGQLGLTEDDQLLPRAGDKPFLEKGQIQLPSLERGRELLTLYFDVCIATYRLLHRPTVETWLATISESARLHKPLFSDIGHAKASVVLSVLAIASFHEDKARGSSAFSAINEGTVRVQSDELFCEAARLTEAESGIPRLESAQSRLIQVLYLLMSCRFNQAWYTFGHTLQIISALGLHRRDDRKWSSTSRKRDYIEEQCRKRTFWVAYILDKYLGVVFGRPRHYHDDDIDQDFPDPVNDQDMTSSGPAVSHADDCHIDALVFHAKLSQITERILREVYTIKSVPDIARLTAAHRLGLELRAWRASLPPLLGAINPSSLIPSFRRQATMLKLSYCHAVMLAHRPFLLKNTTDDSDEMRSLAKDGLAECIFASRSILDAVDRMAREGRLFHAFWFTHYVCFCALVVVYVWMIQESSNTTTRDERRNILDQAERCLQHLAQATASNSPSRKYSIILQELRNEAKRKTAKSAQDQPQPRDLSSTTATQGSESVVSFTDIAQDQSMQDDHAATHTWQPLFDSPIDSAIPGLQNFLDVWQTTDWLHLDSSAFGPFADLDSASVPWMNNVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHB0</accession>
    <name>A0A9W9CHB0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">T-complex protein 1 subunit theta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CCT8</name>
        <name evidence="8" type="ORF">N0V83_009848</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHB0"/>
    <dbReference type="OrthoDB" id="1748577at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03341">
        <property type="entry name" value="TCP1_theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000008">
        <property type="entry name" value="T-complex protein 1 subunit theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012721">
        <property type="entry name" value="Chap_CCT_theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02346">
        <property type="entry name" value="chap_CCT_theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4363552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="555" mass="60445" checksum="8F211978AEC2575E" modified="2023-11-08" version="1">MSLSIPNAPNAGLFKQGYQNYDAEDGAVIRNIDACRTIAQTVQTSLGPYGRNKIVINHLQKMILTSDAATILRELEVVHPAAKLVVMASQQQEAEMGDATNLVIVLAGELLKKAEELIRMGLKTSDIVLGYEKAQNATQQTLEELVCDKVEDIRSQEELSKAIRTVVAAKQSGSEDFLADLVAEAVLAVLPKNPTNFNVDNVRVVKIMGGSLEQSKVVKGMVFARQPEGTITKASKAKVGVFSCPVDISQTETKGTVLLHNAKEMMDFTKGEEQQVEQIIKELHDSGMRVVVAGSTIGELALHYLNRYNILVVKVLSKFELRRLCRVVGATPLARLGAPMPDEMGSIDVVETLEIGGDRVTVFRQENDQTRTATLVLRGATQNHLDDVERAIDDGVNVVKAITRDPRLVPGAGATEMQLIERIKAIADKTSGLAQYSIRKYAEAFEVIPRTLAESAGLDATEVLARLYVAHAAQKGRKDDEWTVGVDIENDDNTGTLDAKEEGILDLWVSKSWAIKLATEAARTVLSVDQIIVARQAGGPKMPGKAQQGNWDQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHU2</accession>
    <name>A0A9W9CHU2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hypersensitive response inducing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHU2"/>
    <dbReference type="OrthoDB" id="3763539at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040754689" description="Hypersensitive response inducing protein 1" evidence="1">
        <location>
            <begin position="26"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="18344" checksum="65570BDF04143BF1" modified="2023-11-08" version="1">MRSTTFLATLLAALLATLLPTAILAAPSSLMARAGDEQCAPTSYTLSDYTLVTSSSQAYVNFNFKSTFADSTIIQDAVTGGANCQADGPTIPNSNECGVEGRKLLFDLRGPEEQAYYQITHTWTCNGATWMSGNAVQVDPLNCNESDGTHTCSGGPQTFAPQNVRKICSTPTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHW6</accession>
    <name>A0A9W9CHW6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein S21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_010222</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHW6"/>
    <dbReference type="OrthoDB" id="2501249at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070124">
        <property type="term" value="P:mitochondrial translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052837">
        <property type="entry name" value="Mitoribosomal_bS21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001911">
        <property type="entry name" value="Ribosomal_bS21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41237">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN MRP21, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41237:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS21M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01165">
        <property type="entry name" value="Ribosomal_S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="14"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="69"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="92"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4363102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="260" mass="29387" checksum="1DC30E51930080E4" modified="2023-11-08" version="1">MSSRSLGELLLRPSSFTRIPPTSSTQYSRRITPSWTARRCISNTPSPAAQPQRKGDTYSTEQPDEEPQQMRQPGQASQAETSQAIDSLFPNRPQSYAPRQQQTTSSDELRAARASHIFGSDWSSPSRSNRPSRRPPGLNFDSMALPDDMLNPSLQNKPSDATALATQQEETFANYPRLNPTYGRTVDLDASRGRDIVRGIGMLGSLMARNKVKSDFNKQRFHERGGLKRKRLNSERWRARFKKGFQEVTGRVTELTRKGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHY1</accession>
    <name>A0A9W9CHY1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009724</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHY1"/>
    <dbReference type="OrthoDB" id="4937900at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053157">
        <property type="entry name" value="Sterol_Uptake_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784:SF4">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="382" mass="42508" checksum="38185AACCE9FB4D5" modified="2023-11-08" version="1">MLRRSHKKSRGGCLECFTSEAPPTTSGASSRSGSPGNLKGPDVTPISDHSPLTPSSLYNAELSQLQGDDSRHNRSLDEPLNFNHLELLAHLTIDKNMFNLGVGLDDYYASLALGLKTSLKWPYLMHQILAYAARHLAFIHPEQSEFYLQQAVTLQTRAVSLFTAIPLEVDQSNCMPILFFSSVLGHHLLADTLGKRDPSGVDGFVDQFVQCAQTYKGVHSIAISAWPLLMESELAPILTRSANFTSREPRGDDCQCVNELVDCTTELCEDDKKACREAIKYLQVGFDAAFEEEPRDGPPNRYQMISEWAMLVPPEFTSLLAAKKPEALVILTYYAALLHCGKHLWQVGDAGTYVIGIIGDYLGAQWDRWLEYPRQTIARSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI02</accession>
    <name>A0A9W9CI02_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TPR-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009246</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI02"/>
    <dbReference type="OrthoDB" id="1936594at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044244">
        <property type="entry name" value="TTC27/Emw1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="603"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="637"/>
            <end position="670"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="934" mass="104224" checksum="1D36EB9B0AF29088" modified="2023-11-08" version="1">MGDVLLQFIRASLPEELAAVLEDVLKAAESGNFLAILELPQVQILLGHEDNESTKETKKEDFGLWNDYIFRRLSQMLADRKSNRNDGEDVPSTEKAMYQRSLLLIVAVAALHAFIQSTVTGPPLPFNSAEALLPKDISGNASILSKTRSDLIASLGADGVAAYRLTADVELLCLAETILICPTIQKNEELSAWARFRVNFVHQRLLSEPAPSLQSAIYDDMESIAKLILSLDKVQAIKDIWPSFLLERASIHTHHGFDKKARVDLEEATTERKFEFALTGLMGKRTKFQQKDTSQLLVLARSAGSETNGTANEASKPTALDLNDDTLLESISFTENTASTDIKDESALPASLTSMDPSNQPLLDPLDSVILLSLAESIKNTNPVDGLTREQTVPYAERVLEGGSSNWQIYTQALLVRSRIEGYKSRTMERGLLQLQALVDQVIAETSGDGTTDAETGEKVTSFLPQAKEGEAASVEERLRYVFPLCSPSRWELEAELAARWVHIGGLRSALEIYERLEMWAEAALCYAATEKEDKARRMIRRQLFHATNGDDDTADLDEETWEGATRDPPPAEAPRFYCILGDIDQDLSMYEKAWEVSGRRYARAQRSLGQRYIAEKNYEKAAEAYSLSLKINALYHPAWFALGCAQLELLQFKNAVEAFSRCVQLDDQDAEAWSNLAAALLHLRPKAKTEVEGEDESPARRVTNHPRTDALKAFKRAANIKHDNYRIWSNVLAVAPSTEPPSWNDVVTAQRRICEIRGSTDGEKCVDAEVLDMLVKDAVRSEQVFDISRPGLPRMINELIEKHVKPLITFSPKLWSILATLYTHTSRPSSALECHEKAWRAVTSQPKWESGTEAEWNAVVDQTIDLVDAYETLGPREKTEGLAAGTGELVAKDWKFKVRSAVRSVMGKGRENWEDGDGWERLKGRLDELKGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI26</accession>
    <name>A0A9W9CI26_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">arabinan endo-1,5-alpha-L-arabinosidase</fullName>
            <ecNumber evidence="4">3.2.1.99</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Endo-1,5-alpha-L-arabinanase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_009788</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of (1-&gt;5)-alpha-arabinofuranosidic linkages in (1-&gt;5)-arabinans.</text>
            <dbReference type="EC" id="3.2.1.99"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism; L-arabinan degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.99" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI26"/>
    <dbReference type="OrthoDB" id="195678at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046558">
        <property type="term" value="F:arabinan endo-1,5-alpha-L-arabinosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18831">
        <property type="entry name" value="GH43_AnAbnA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050727">
        <property type="entry name" value="GH43_arabinanases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006710">
        <property type="entry name" value="Glyco_hydro_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016840">
        <property type="entry name" value="Glyco_hydro_43_endo_a_Ara-ase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43301">
        <property type="entry name" value="ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43301:SF3">
        <property type="entry name" value="ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04616">
        <property type="entry name" value="Glyco_hydro_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026534">
        <property type="entry name" value="Endo_alpha-L-arabinosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity, responsible for pKa modulation of the active site Glu and correct orientation of both the proton donor and substrate" evidence="9">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR606710-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR606710-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4363493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="283" mass="30513" checksum="16005F11ED245127" modified="2023-11-08" version="1">MACSGVCNNAHDPALIRRDDGTYYRFSTGAKISIHSAPSITGPWTYKGAAIPSGSSIDLTGKDDLWAPDVTKVGDTYYLYYSVSSFGSQNSAIGVATSTNLDSWTDHGSTGITSKAGKNYNAIDGALYWDGSKFVMSFGSFWGDIFSVNMANPPLKVLSGAASTNIAYKPDGSHAQEGAFIALNGNYHYLFFFCRPMLRLRYQQASRGGFVDKAGKKCTEGGGTTVLESHGWVYGPGGQGVYYDPSLGPVLYYHYVDTRIGYADGQKKFGINKIDFSSGWPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIB8</accession>
    <name>A0A9W9CIB8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phosphoribosylglycinamide formyltransferase 1</fullName>
            <ecNumber evidence="2">2.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ade5</name>
        <name evidence="6" type="ORF">N0V83_009941</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.2" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIB8"/>
    <dbReference type="OrthoDB" id="5575075at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.170">
        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43369">
        <property type="entry name" value="PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43369:SF2">
        <property type="entry name" value="PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl transferase N-terminal" evidence="5">
        <location>
            <begin position="8"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4363645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="192" mass="20671" checksum="ACC9E5A5E182E541" modified="2023-11-08" version="1">MADTTQFNIVVLISGNGSNLQALIDACASGTLPNTRISHVVSNRKAAYGLTRAANASIPTTYHNLVPYKKKHSPDDVPRAREEYDADLAQLILSLSPRPDLIVCAGWMHIVTSAFLTPLSAPPSNTQSTDSSDPIPIINLHPALPGEFAGANAIERAWRAGREEGLKRTGVMVHEVIAEVDAGRRLLLGKWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIJ6</accession>
    <name>A0A9W9CIJ6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA (guanine(9)-N1)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.221</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA methyltransferase 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA(m1G9)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TRM10</name>
        <name evidence="11" type="ORF">N0V83_008220</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>guanosine(9) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43156"/>
            <dbReference type="Rhea" id="RHEA-COMP:10367"/>
            <dbReference type="Rhea" id="RHEA-COMP:10368"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.221"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.221" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIJ6"/>
    <dbReference type="OrthoDB" id="278300at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052905">
        <property type="term" value="F:tRNA (guanosine(9)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18089">
        <property type="entry name" value="SPOUT_Trm10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028564">
        <property type="entry name" value="MT_TRM10-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038459">
        <property type="entry name" value="MT_TRM10-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007356">
        <property type="entry name" value="tRNA_m1G_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563">
        <property type="entry name" value="TRNA (GUANINE-9-) METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563:SF13">
        <property type="entry name" value="TRNA METHYLTRANSFERASE 10 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51675">
        <property type="entry name" value="SAM_MT_TRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase TRM10-type" evidence="10">
        <location>
            <begin position="134"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="9"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="361"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="10"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="87"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="369"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="379"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4365600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="422" mass="47173" checksum="9F941FDFD17ACF39" modified="2023-11-08" version="1">MIFKIVVMESEERPAKVRKLEHADDEHSIPAANVAPTSTTSDVPVPVISENAESEESDNAATGTTAENDDAAPKLSKNQLKKQRRHERWEAGREDRKLKRKEKIKEKKERRREAWKSGNTDENGNVARPPPARLPRRTAGLGTQVPVTIIFDCDFEDLMFDHELKSLGLQITRCYSDNRKAKFRAHLALSSFGGKMKERFDGILAQQYTSWKGFRFFEEDFVDVAQKSKEWMADAKGGEVAGALKPLEENKTSGEQGNTEGTAEEGEIVYLSAESDNVLTHLKPNSTYIIGGLVDKNRHKGICHKRAVSRGIKTAKLPIGEFLEMNSRQVLVTNHVLEIMLKWLEFGDWGKAFMDVIPKRKGGRLKEGGDDEDADTYEADDKERLDQDMEDAKQEAEDASAEATADVPASAEVVEAEKAIAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIK8</accession>
    <name>A0A9W9CIK8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Eukaryotic translation initiation factor 2 subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_009062</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF2B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIK8"/>
    <dbReference type="OrthoDB" id="1685042at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1130:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000015">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.190:FF:000002">
        <property type="entry name" value="Translation initiation factor 2, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="entry name" value="Translation initiation factor 2, subunit 1, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="eIF-2-alpha, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="eIF2alpha middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="17"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="283"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4364468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="311" mass="35144" checksum="DF384A85717DCB8A" modified="2023-11-08" version="1">MSLTNCRFYEEKYPEVESFVMVNVKQIAEMGAYVKLLEYDNIDGMILLSELSRRRIRSIQKLIRVGRNEVVVVLRVDKDKGYIDLSKRRVSPEDIVKCEERYNKSKMVHSIMRHVAEKTQTPIEDIYQSIGWPLNKKYGHSIDAFKLSITNPEVWNDVTFPNDVVRDELQSYIGKRLTPQPTKVRSDIEVTCFGYEGIDAVKTALRCAEAKNTPDNQVKVKLVSPPLYVLTSQCLDKSAGIACLEQAIIDIKENITKAGGDCQVKMAPKAVTENDDAELAALMEKRERENQEVSGDEDESTSDEGGVAEAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ41</accession>
    <name>A0A9W9CJ41_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA mismatch repair protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MLH3</name>
        <name evidence="4" type="ORF">N0V83_008690</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ41"/>
    <dbReference type="OrthoDB" id="429932at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032300">
        <property type="term" value="C:mismatch repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.20">
        <property type="entry name" value="MutL, C-terminal domain, dimerisation subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014790">
        <property type="entry name" value="MutL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042120">
        <property type="entry name" value="MutL_C_dimsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037198">
        <property type="entry name" value="MutL_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF47">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00853">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118116">
        <property type="entry name" value="DNA mismatch repair protein MutL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MutL C-terminal dimerisation" evidence="3">
        <location>
            <begin position="719"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="428"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="894"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="430"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="894"/>
            <end position="906"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1019" mass="111177" checksum="336858851FEEB8A2" modified="2023-11-08" version="1">MTQAQDIATTPTGEGRSILPLPAEVVAQLKSSTAIVSLTGVVLGLLSNSLDAGATKIEATVDFARGGCTVEDDGLGISPVEFREEGALGKPYCTSKFYSHDAFLGRNGSFLAALGAMSLLTITSRHHEHRSQNTITFLHSKTTDRQLPASAQHQLHSKHGTRVTVRNLFGNLPVRVKQRPVVTDQKTELDRLWHELKRHVTCLLLSWRGVASLKIRDSDNKALISFNSSSSVIATQHHDKNITSPHSAQLSSILSLLTQAKYITIDEWTSWVPASASTSALSIKGAISLDPAPSKHVQFISLGLRPLSPESGQNELYDIVNRLFALSTFGTVEEDADVDDKELVRRQTDKRFKRDGYAIRQLKARKDIDRYPMFHLRICLKGDHRTSLSEDQLLADESSLQAVVEVLSAMITQWLSIHHLRPKQAFQRRGLLDKSDSPCRDNKGDKQSTADDRISTRAHVRASRTTPSVTPAPVRPGSAVSATRKKMPPPGTISRESYEGQQHGAFAEWSRIKSANPTFFSNLPTLAKLPVGSTLTAEKPEMLFDFKVGPVPLGAYGASQDAASPSTSASKHCAGNDESDATILWADPSTKRTYTLNARTGCVMLDARSRPNIDSALLPSGARQRELCSSLRLAPKSATALPTETPWLDSMLETWENPIFQPSEKRIQKISLEEHDFEAGTQHISQDRCSHFTYDKASSGASAAGASRLSKQALQKAKVIAQVDKKFILVKMQNAPLSGSHSKEDRELLVLVDQHAADERIKVESLLRDLCTPLTSDNVRSGYQSKLGHKAQVAAIALGKPIQFTISPQEKPIFVTHAARFAAWGILYDTPISPSSTIPPCPTSRKCQPVLSVTSLPPSISARCTSDPQILINLLRSTVWSYAGDAHLPPLPSSLSTASLPSAPSTPDEKEDNTNWLNRLATCPQALIDLINSRACRSAIMFNDELSYEECEALIVRLADCVFPFMCAHGRPSMVPLGDLGGKEDGNGDAVVGLGSGKAVDSEGNGFVRTWKAWREKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ46</accession>
    <name>A0A9W9CJ46_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein SOSEKI 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SOK1</name>
        <name evidence="3" type="ORF">N0V83_008705</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TCP11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ46"/>
    <dbReference type="OrthoDB" id="276323at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010737">
        <property type="term" value="P:protein kinase A signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008862">
        <property type="entry name" value="Tcp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832:SF11">
        <property type="entry name" value="LD23868P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832">
        <property type="entry name" value="TESTIS-SPECIFIC PROTEIN PBS13 T-COMPLEX 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05794">
        <property type="entry name" value="Tcp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="592"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="606"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="641"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="655" mass="73386" checksum="2EA5BA863EA612FA" modified="2023-11-08" version="1">MDVDSRNTSASPWHRSLIRTESPLAGEEKCVERGRRSSTSEEPAARDAYLPPPTPPHHQNIPEQLAELLITMIGERKVGELSGEDLAEAFRDGPDVPPVTTQSLSELDIQSIITNIKLRHDVNFDRDLSFRPNVDGVKGIEKKRAQDKYKNALIAELTLYGLLSQEAPPLRGIEHMKPEDITYHAERRLPTLFHTIREVLKSLVPDRDHTRVEEHLDVSMLMQEIERGVCDMVRLAEWIAHLLKEHCAPMRDDLVDNMVTSTKAGVSQNDVTLIVRGLSELLGILEAMKLDVANHQIRNLKTILIEDTLNFEKHYHLDRLVHGRSRVNVNTTQSWYADANDEFGSQCTPRSRDVSGFQLEVFTRAVVAILFGRDGRSNFPDTFYLDHDRLRLLQAEIDDLVFVEICMEMFAVLAKELGSSGTITLAIGQELRTSISAIMGEASDLRPQQWMMNSEALSLEILRQASRLAGRSPTYHFDRLADANQHLRRLFVNNFAQHAAQLEAALLPQILGSADRHGSSSPMELFNSLVSVSSSTPLPPYPAQPLLSDTCAFAPLHQEIAKLTDIANRITHIVLLHWRVWGHIAYLQDDDSQQSSIVPSDDEMSRSSISPHPTLSHCSQPHVSDEAAQVVATMKTGEPLDSSQEAHVAHQTPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJP4</accession>
    <name>A0A9W9CJP4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007919</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJP4"/>
    <dbReference type="OrthoDB" id="1708389at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037847">
        <property type="entry name" value="GRAMDC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37402">
        <property type="entry name" value="GRAM DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37402:SF1">
        <property type="entry name" value="GRAM DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="311"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="622" mass="70778" checksum="949EA09699ADF66E" modified="2023-11-08" version="1">MVPEDEYVQVDEEQIGLDRAIITSKGQIQLPQLGDSQRHDDGPRQDPSRHVELVEPDQRSSTQHMKDSIKLKSHNAGIKIRKTFHISKESDRHETLNPPILANTAEETSDSRLDNASPTSDEHTLKDLVHNPVDTIRSKLSNQGSQQMAVNIATKEIAHGQEVDLVNASTAVERADTENDKLRAIQTLSELLKQRQSTYVRWTLDRHVTKVRIIPRETIVRRPKTDFERKNASGEVIMDWHAYVKHLLEYYAHQYGGQYIGYGSDPPAPSKETIMPNVERLIVASSPLQEFVMTTRRVYRWENRAETTKYLLIYFTLWYFNLMLPGILSAIAYFVIQRHIHVNSMENLREDIKHREDVQRTALSLTELIVKEGDEEWSDKLLEGMGPWAMIQLADLANMCESIRNFYEWRKPTRTKALLGVLGLAIIITAVMPIWLLVKATTLGAGISFFGLFPIAVNFPEYRLLVSPTKRLLWNIPTHAEWAIKYIQAEGSRVEASAEPAPSALPIKKDTSSAKAEDYGFYNAHHDKSSGHLVVSTTSVRFVTKHPHAVEFTLPYDQINKIEKLDRIVAKSLSGKMTGDSGKDLKLVSKAGQEWVLKDVELRNEAFSQIVGFSKTTWQVVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKG1</accession>
    <name>A0A9W9CKG1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_007602</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKG1"/>
    <dbReference type="OrthoDB" id="1638493at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4367072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="41259" checksum="F5C4CF98D6259444" modified="2023-11-08" version="1">MLFEALATELVLQIYYSCASITDVLTLSSTCHRFRNIYASSQKLAILETVAESQFGPLQDLTQLLTHNASQAVHVIRDVPFSAALLKQIVHTGRVAEKWCDVYPFKKWKYNYESRRLLTSDERYRLRRAIYRTWLYSRAFHNRDHPRELRATRLSLQKRAALLHNWNTWELAEIADVHAVIREVVHSNICPSNGTIARKFRKRHPENEHGLLFNIHLNYPPPAPPAYNPFAANSLSSHFNNTSTYTSRYASSKYSLHPSHEVGAEGWGDDIPHYYVIEDMLKMDPEQILWLKENAPLKGMVETYVRSLGENYDGSNWFTNNGETLVQTLEFVLDERGEQVGEFMGAIAEGELGVAVNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKU5</accession>
    <name>A0A9W9CKU5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_007098</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKU5"/>
    <dbReference type="OrthoDB" id="294251at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000026">
        <property type="entry name" value="TBC (Tre-2/Bub2/Cdc16) domain family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000046">
        <property type="entry name" value="TBC domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="799"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="320"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="411"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="575"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="648"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1071"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="197"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="38"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="343"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="436"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="469"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="576"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="596"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1082"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1115"/>
            <end position="1130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4367515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1162" mass="126707" checksum="BB9BEE64EE1351C5" modified="2023-11-08" version="1">MAAIDAPRAVDLVRTASQNSTASNPRNRSIQRVRPSKRSSNASLSRSRSRAPSPGDDTKSLTSFPSLSPSPDASPVQSRINGWFRSAPAPDETVKNADSRPTDSPDTVKAVTPPEPGKNTVRKVTKSTQQIVGTLVASSSKKGRSALFDDTPAEPTKVPGNLHFATDENIRDALGQTGAVTLVRQLAGDLAQRDAQVTALRRRAEERERILRRMLQECEVSNMDIENRLRELDKSKEQPNGLAKIRTRGDSSLAGLHPDDPMEDRLARAMEDEVAEHPDALGFDSGTAAPMADVMSINSVASDTPRDTTRPNWKSYIWNGTSRKSSRAPSVASMVDRDAENMAGRSRSRASSGAKPPRKALANDLFVPPTGAVPALRRLHSQEHTADQSSRRSSSVSLTNWALKMVAGNASAAANKSGTVRGRKNTISDAADRTPSMASMDSTRTGQSAKSSLVATQRRGRALGPNGTIKSISADPSKASSSTVGPNPPTRGMSNLGPVEMDRILPEESRPPTLVQHHNRFVGSSEYLTDRFGFIYDQRRKKRQSEAAAEIVKQKRGSNVESLGDGRSVLLKLGELDEENEDVNDVSDPNGSATRPSSSGSDDQSGKQPTKTWQDYLKLATHPTELLSHTPSSAPITAVTVESPEAEIVAPKAGQSQITLTKRGSLPPSSVNPEPSPSRIFSGNAELSVTTTSPGPSTPVSPYPPQIDPVKSLLDQMSELHDNLQKERTIKWNEFLRKVRAQNKREGDVAYTGESGTKGLSMPETFLADGEVIGIAGLGNKGKVGRAKWQEFRRLVLDGIPVTMRAKVWAEASGAATLRVPGYYQDLVNNGEDDPTIASQIQMDITRTLTDNIFFRTGPGVQKLNEVLLAYSRRNPEVGYCQGMNLITACLLLIMPTEEDAFWVLATMIEDILPQHYYDQHLLTSRADQSVLRAYVGELLPRLSAHLDELEIELEALTFQWFLSVFTDCLSAEALFRVWDVVLCMHDGSTFLFQIALALLKLNEKALLQCDTPAGVYHYINHQMTNHAISIDGLIQASDALGKVIKRKDVEERRARAVAHEQELMRQRAEARLERAKKRASKVSTDDAASAVSAPPPQPQDDAKSLSASLDAELSMQTSPMRTPLSSASMRSEEEEHADELNGSLELAGRTPMPMEEECLWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKV9</accession>
    <name>A0A9W9CKV9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NFACT RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_006560</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKV9"/>
    <dbReference type="OrthoDB" id="200398at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039730">
        <property type="entry name" value="Jlp2/Ccd25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13049:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13049">
        <property type="entry name" value="DUF814-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="3">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4368204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="213" mass="25150" checksum="39B0292F51D9D05B" modified="2023-11-08" version="1">MVYYFSSNTVTPSAFIYVGKDKVENEELIKYGWDEDVWFHADNLSSAHIYVRLPEGEDWEKMTKELLIDCAQLTKANSIEGNKKDNVTIVYTPWSNLKKDGSMAVGQVGFKDQRKVKRIHVEQRENPIVNRLNKTKVEKFPDLREEKEARLSELRKRDRDALQTRRKEEARIAKERKELAWQRDHAYDDVMTEDNLAQSSNQDRDENFLDDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL85</accession>
    <name>A0A9W9CL85_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">TMEM1 family protein-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_005401</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10256906at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055505">
        <property type="entry name" value="DUF7077"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045126">
        <property type="entry name" value="TRAPPC10/Trs130"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022233">
        <property type="entry name" value="TRAPPC10/Trs130_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056913">
        <property type="entry name" value="TRAPPC10/Trs130_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13251">
        <property type="entry name" value="EPILEPSY HOLOPROSENCEPHALY CANDIDATE 1/TMEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13251:SF3">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23274">
        <property type="entry name" value="DUF7077"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12584">
        <property type="entry name" value="TRAPPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23036">
        <property type="entry name" value="TRAPPC10_1st"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24965">
        <property type="entry name" value="TRS130_4HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="TRAPPC10/Trs130 N-terminal" evidence="6">
        <location>
            <begin position="125"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="TRAPPC10/Trs130 N-terminal" evidence="6">
        <location>
            <begin position="275"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7077" evidence="7">
        <location>
            <begin position="1033"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="domain" description="TRAPPC10/Trs130 C-terminal" evidence="5">
        <location>
            <begin position="1378"/>
            <end position="1535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="93"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="197"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="568"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="112"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="199"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="568"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="582"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4369639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1564" mass="173362" checksum="D1D7A61ACE6138BA" modified="2023-11-08" version="1">MSVAGNGAPVLGAAEDVSRQNHAEPTVMDGLSSSKVTVEYHDPSGVFPLIQEDLAARLPLRNLHWKAPTRPLRSIDSLHVDLVPSKESAHASGGAALALASSQGNQGTPTRTTDEILRPPTKEKERRHQIPGLRQTPYLKVYLLRCDDSETYKATARKQVREWVKEHTPPSQSSSSSSTQENHDAHEWMILHVVLPDTPASSQPRGSSGSATGEKEKGPRWARGTSTLLEKLRADFNISSKSAPDRVAQIRLQKDRVPPHLLPAPAPANAPVTESPQEQERAWTDVIAKFKTLILLSFDLRVSQYEEDIRKNDAQRSFPGWNFNTFFILKEGLARGFESVGLVEDALLGYDELSIGLDTVIRDQASEGTGTGVLLNYSEDLYEKTTKLLQQTKDDGEDREELQAFFQDTKPINAQNKDYRGLILANNISIFDFRMYIFARQMFLLLRLGNSLSARSDLAAKLQPRPNATTLQRSVDDGAVGMKSDNQPNDSEDLFSFAELCSRALNFITFAGRLLRDDLVNGAKAHKTTFPAHLIDNVVRSWTFAAIEQVLRETSTASLPFTRFSKDLSVGSSGKSLSFSGRNKEQKIRVSEPKSMIHPSRSSSLNHGRPTADAPYAQPTVSSQVVFENGQYHDRPPPAQTSSIPSAKNGLQDLAGTRAQLLVVQRRLLEHIGKALGWNIGWAAVIPSLNLEEELNDVDLDEKEEDASKENSTDALEGSQTMAPVAGILAEVLVTAVSSVDQFRQYYESLSDLIVKHYMAAGQTKSAESVLGDLAALRFELGDFAAAAMYFGRMASLFAESRWNTVETTMLKMYARCLKKLNRKDEYVRTLLDLLAKSAASRMSFKTSTKQASADEASDIHRDWLNDDKVDTTGVFHELIEYSQQLPYDVTVQMPKYFGDISVEPYVRHYEDKDGFQLRLHFRHLLEDEVEIRAAKVRLVSATSTQGKEIWLENTEPMQLKKGLSRMWLGCMINTTGPYMVDKIVLEAKRIVFVHEPFTKTEATTPLGIITSVSAHALKAAKKARVLCFPRAEAFQARIYLSHFLHIDKPRHIAVECTSGWNEITHAEIRLKSASAGLRLRTANANVTAGETNIESKPNPGVIAVGAMAAESTATIQVPYDMETILQDLTVKIEVDYYTAGGQFQYISSFTIPVDLPLDVNVHDHFKSNALFSKFNIKTANQVPLELLDVELQSSEDFDVHAPRKPQLPVNVFPKQPLAITYKITKKAADAATKRQSRLPSTASLSLSVEYRCLNEDVLDRVRKLFADAVTNSPVHRLARLLTDTFASRLEQSILPRQFERIALLEKLDMGYFEDMGWADCLDSLPHIIRDDTQAWLQKWHEQHRTILLSKIASGESAPPPAPIAPASPSPPRRMIITVSIPQTHILHTASLALTSPPLLPSLTAIATVGQPLMATLRISHTRRWASPSSLISAANISAADEPLDFVYTIEANPETWLIGGQRRVQFSAKEDEVREWMVMLVPLKQGMALLPAVEIRAKVRAREEGGDGKTGAGDEVVLNSETDYLSHGESVMIVPDVRSSTVGVGDMRLGSPKSVVWLESLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLA2</accession>
    <name>A0A9W9CLA2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD synthase</fullName>
            <ecNumber evidence="2">2.7.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">FAD pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">FMN adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">FAD1</name>
        <name evidence="15" type="ORF">N0V83_006608</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>FMN + ATP + H(+) = FAD + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.2" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLA2"/>
    <dbReference type="OrthoDB" id="270728at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23948">
        <property type="entry name" value="FAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000187">
        <property type="entry name" value="Probable FAD synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293:SF9">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293">
        <property type="entry name" value="FAD SYNTHETASE-RELATED FMN ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="14">
        <location>
            <begin position="69"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="14">
        <location>
            <begin position="153"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="231"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="231"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4368252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="282" mass="31562" checksum="BA6541D4EAD25BDB" modified="2023-11-08" version="1">MNTAAGFSDPLVTDTPLPFHELCARIHGRIAVFLDAEDVSDRVKSVQEQTRVSLNVIDQALEQYSLPELSLAYNGGKDCLVLLILYLCALHDRGLTRTDNPSSNIDTAVQCVYIQDAHPFAEVEAFVAQSIKTYSLALLEYAKPMKAAFADYLRDTPSVKAILVGTRRTDPHGEHLKHFDPTDSGWPKFVRVHPVIDWHYVDIWTFIRYLNIPYCTLYDLGYTSLGGTTDTQPNPALARQSSSQQVPQLDAANGAVNGTSKPKFRPAYELVDDYEERLGRDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLB4</accession>
    <name>A0A9W9CLB4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phthalate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_006080</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLB4"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000018">
        <property type="entry name" value="MFS transporter permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF47">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="351"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="408"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="440"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4368998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="486" mass="53915" checksum="2A5EBF341A4999D3" modified="2023-11-08" version="1">MTNTTTKDEAINHIEKVEKGLASPTASSEHTFDTKLAASVRHRVDWRLIPALGAMYGISLMDRKNVSNAAIAGMLTDLKMGRGMAYNLVNLCFFISYVLCQPFMIVVCRKVGPRYFLPGICIAWGTVIIGFGFTKTWEGLVPLRLVLGLLESGYFPGCLYLLSCWYTKFEVAKRYSVFYFVGSIASALSGILAYGLQQMEGVQGIRGWRWIFIMEGVITCGVAIFAVTFIIKFPDQEKKNPSWSFLKPHEINLIIDRLNADRGDVEAEKFSWKKFFEPAKDWYIYAFAFILLFITTIAYGFAFALPIILSTKLKFSVAMSQCLGAPPYAASGFMMYGAAWFSDKYKTRGPVLCFLCVISLIGLPIMGFVKNPWAQYVGVFVTVSGTNSAIPAVFSYQANNIRGQWRRAFCSATLTGIGGIGGIAGALIFRTEDAPMYVPGFAACIACNVLVILIVAVLTVYFRRCNAQADRGERVLLGDPTFRFTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLS7</accession>
    <name>A0A9W9CLS7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RNA-splicing factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CWC21</name>
        <name evidence="9" type="ORF">N0V83_005582</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLS7"/>
    <dbReference type="OrthoDB" id="10267305at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21372">
        <property type="entry name" value="cwf21_CWC21-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051372">
        <property type="entry name" value="CWC21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013170">
        <property type="entry name" value="mRNA_splic_Cwf21_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36562">
        <property type="entry name" value="SERINE/ARGININE REPETITIVE MATRIX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36562:SF5">
        <property type="entry name" value="SERINE_ARGININE REPETITIVE MATRIX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08312">
        <property type="entry name" value="cwf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01115">
        <property type="entry name" value="cwf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="CWF21" evidence="8">
        <location>
            <begin position="54"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="95"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="27"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="115"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="142"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4369818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="182" mass="21187" checksum="DDA4C87F30FEB387" modified="2023-11-08" version="1">MADNVGLTTPRGSGTSGYVQKNRSLLRPRDKVAPYPKDWESAKHRPRQPDAEILEHEAKRGVEVKVLELRDKLEDEGVDEDEIDDQCEALRQKLEQERKDGKEMGPNSKRLKSHQVHDLAKAKIEESERLRKALGISADYEEGSHWRKQEERMRESLAKREAEDEEKLERAKEARRYKEDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNC6</accession>
    <name>A0A9W9CNC6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_004816</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNC6"/>
    <dbReference type="OrthoDB" id="3783451at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4371596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="231" mass="26229" checksum="8CD9919FC5A54301" modified="2023-11-08" version="1">MRDTMTSRLDDMGGRIRQDLLYRLRWNLLAALDNIDITTGPQDEESVVPFFSDAAAEESIAQPPLSRVEVRIDDCLANRLEEMYFDEEDQSNPPADLTIENADGSPITVGQFVSQVHPYMNEHMEENKKVKGEIYGRAEKREDGTEVRVNIYGQPYLPPDIGFFFSRVWAVGHNDYVRLNVSSFAEGEIGTSVDDFWAIQLRQARMCEQKRYGEQATIESLPLSEPGNTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CND6</accession>
    <name>A0A9W9CND6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial</fullName>
            <ecNumber evidence="15">7.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">NDH51</name>
        <name evidence="18" type="ORF">N0V83_004846</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Complex I is composed of about 40 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Mitochondrion inner membrane</location>
            <topology evidence="15">Peripheral membrane protein</topology>
            <orientation evidence="15">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the complex I 51 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.2" evidence="15"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CND6"/>
    <dbReference type="OrthoDB" id="42889at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.230:FF:000001">
        <property type="entry name" value="Mitochondrial NADH dehydrogenase flavoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.600:FF:000001">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11540:FF:000001">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 51kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.230">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 51kDa subunit, iron-sulphur binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050837">
        <property type="entry name" value="ComplexI_51kDa_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001949">
        <property type="entry name" value="NADH-UbQ_OxRdtase_51kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011537">
        <property type="entry name" value="NADH-UbQ_OxRdtase_suF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019575">
        <property type="entry name" value="Nuop51_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037207">
        <property type="entry name" value="Nuop51_4Fe4S-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054765">
        <property type="entry name" value="SLBB_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01959">
        <property type="entry name" value="nuoF_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010120">
        <property type="entry name" value="PRK13596.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11780:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11780">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE FLAVOPROTEIN 1 NDUFV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10589">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22461">
        <property type="entry name" value="SLBB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00928">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="Nqo1 FMN-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142984">
        <property type="entry name" value="Nqo1 middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140490">
        <property type="entry name" value="Nqo1C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00644">
        <property type="entry name" value="COMPLEX1_51K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00645">
        <property type="entry name" value="COMPLEX1_51K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="15" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 15" id="KW-0288">FMN</keyword>
    <keyword evidence="10 15" id="KW-0408">Iron</keyword>
    <keyword evidence="11 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12 15" id="KW-0520">NAD</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="15" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="15" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADH-ubiquinone oxidoreductase 51kDa subunit iron-sulphur binding" evidence="17">
        <location>
            <begin position="390"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="26"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU364066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4371626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="507" mass="55474" checksum="37FF533FC607F86E" modified="2023-11-08" version="1">MLARHAPTPASLTSAAHRALPAASAATRLATSASPSRTQSRSLATVNDAPPKRTYGGLRDQDRIFQNLYGHHGADLKSAMKYGDWHKTKEILLKGHDWIISEIKASGLRGRGGAGFPSGLKWSFMNFKDWDKDSKPRYLVVNADEGEPGTCKDREIMRKDPHKLIEGCLVAGRAMNATAAYIYIRGEFYHEATVLERAIQEAYKAGLIGKNACGSGYDFDVYVHRGMGAYVCGEETSLIESLEGKPGKPRLKPPFPAAVGLFGCPSTVANVETIAVAPTICRRGGSWFAGFGRERNQGTKLYAISGHVNHPCTVEEEMSIPLRELIDKHCGGVIGGWDNLKAVIPGGSSTPILPKHVCDDQLMDFDALKDSQSGFGTAAVIVMDKSTDVVRAITRLAKFYHHESCGQCTPCREGSKWTHQIMDRFYKGQAREREIDMLQELTKQVEGHTICALGEAFAWPLQGLIRHFRPELEARIKDYGLENGGEALAGGWQHDAAKRGQLIAPGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNK2</accession>
    <name>A0A9W9CNK2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alpha-1,3-glucosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ALG6</name>
        <name evidence="11" type="ORF">N0V83_003931</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ALG6/ALG8 glucosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNK2"/>
    <dbReference type="OrthoDB" id="5589195at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042281">
        <property type="term" value="F:dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004856">
        <property type="entry name" value="Glyco_trans_ALG6/ALG8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413">
        <property type="entry name" value="DOLICHYL GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413:SF1">
        <property type="entry name" value="DOLICHYL PYROPHOSPHATE MAN9GLCNAC2 ALPHA-1,3-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03155">
        <property type="entry name" value="Alg6_Alg8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 10 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="240"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="340"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="408"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="441"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="504"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4372158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="543" mass="60660" checksum="D6C8E7AF58880A06" modified="2023-11-08" version="1">MEHGSLAHVAHGTLDLDASTNARTPAFPLVAFLWPAKGTVSQWVTIPLILMAVGLFRWATSLWAYSGLDSPPMHGDFEAQRHWMEITRHLPVSRCGSAINPEWFALDESRGLDDPNLKTYMRATVLVSEYLICIPAIVIFLRRYSRLEGVNIWESSIALVAVLMQPGAILIDHGHFQYNTVMLGFAVATLSSMVAGRPLWGCVFFVGALGFKQMALFYAPAVFAYLLGICVFPKINVVRFLAIALVTLAAFAVLYLPFLLGITYDMYRGVSYDKFPLPPLMESLPIRWNTKAWYYPIALQLTQSIHRIFPFSRGLFEDKVANVWCAVHTFHKLHRYPVELLQRLALVATSAAILPPCLIIFLSPKKQLLPLAFAAVSWGFFLCSYQVHEKNVLLPLLPMTLLLSGKDGLLPSTRAWVGLANMLGVWTMFPLLRRDGLRIPYFVISLLWAYLLGLPPLSFSAYHEGSKSGLNLFSKILHVNMYVAMVGWHVVEALIPPPTNKPDLWVVANAIVGTGGFCICYLWCLGRLSVKSGLFGKEKSKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-06-18" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNZ6</accession>
    <name>A0A9W9CNZ6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Negative regulator of mitotic exit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">KEL2</name>
        <name evidence="8" type="ORF">N0V83_002599</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061245">
        <property type="term" value="P:establishment or maintenance of bipolar cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000049">
        <property type="entry name" value="Cell polarity protein (Tea1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244">
        <property type="entry name" value="KELCH REPEAT DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244:SF456">
        <property type="entry name" value="MULTIPLE EPIDERMAL GROWTH FACTOR-LIKE DOMAINS PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="432"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="761"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1277"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1358"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1417"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="809"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="921"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1020"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1135"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="61"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="522"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="592"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1280"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1293"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1359"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1421"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1438"/>
            <end position="1447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1448"/>
            <end position="1468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4373860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1468" mass="161854" checksum="6BB1719B2CD3B6EA" modified="2023-11-08" version="1">MSFLFGKKNKQQPPGALPAATREISSSHGQGPTPPAINGGLRDVEKNRGGPQTQTPTPGGSVNNSLSSLQNPGNVTTPEPKALRDRNVNGKGLGNSPDSPYPWSSRRLNFTAGNPFPRYGAAINATASKDGTIYLMGGLVGGATVKGDLWLTEMGNGSMACYPISTTGDGPGPRVGHASLLVGNAFIVFGGDTKLADNDDLDDTLYLLNTSTKHWSRALPQGPRPTGRYGHTLNILGSKIYIFGGQVEGFFFNDLVAFDLNSLQSSASRWEVLLPNSKDQASPQGRSPPARTNHSVVTWNDKLYLFGGTDGITWFNDVWTYEPRSNAWTELDCIGYIPVAREGHSAALVNDTMYIFGGRTQEGVDLGDLAAFRISSRRWYMFQNMGHSPSARSGHSMTAFGKHIVVLAGEPSSSVSDRNELSLSYILDTSKIRYPPNENAPPQQPQNAPRKLSEGERSNIPPSKAGSPPARESTMLGPQLARSPDAIGSGGGVAGSRLPRAAGQTPLGPPPIQQLPQPRVNGPSSQVNANPRSKTPTKNDRMYGPVVDTGVTSSLDRDGFSPMTRDSPSTNEVQRKASDAISQRTPKESMDTSRSGSIVSRNTTRSHRQQPSQDSVEQGALRRSSENQEQYTMNREAENPLVDSGLGASPAVGQYPEEVNRELEKAKLRNAWYASELALARRAGYNVGTSASPMFDERSTDAFGEENRPLLEALLRMKTELERVQSSIKSQGEDAARRIAEVEMQRDAAVNEAIYAKTKLAAHGGGSHSGTPQPDGPRSAGTPDMDRIHDVNRRLAASLATQGELFSKVEQLTHQMDAERKAKQLAEDTAEAAQKRVRELDSTRQKTAAEFESLRAELHEAERVAREVSTNAAEAETSSKLLAIDKQELSAKVARISEESKSHSTLLQSLRDAVLASSEKSALLEKKLEDERHQSDSLRQKLAQLRTEHDAYATELETATQKLRDTEELAEKHAEEARMHRQAVLSGLGNMANRSVVKNDSVDERVVVLQQQIEAANSMTRKNQLAADQASEKLRRAEERIAGLEAYQEQSSREGLTIRKQLQQAMRDVQTSEAEKADLRQQHERLRLDSNAYEVQLRTLKNLLEERGVNPVDARRSRVLDSPSSRFGTPELNRVRELEQQVDSMNKAHEELRIAFEEREQEVSKDWEEKLQALHNDHQAAVKYLRGTEKMLAKMKQELDRYKTANSKLEEELIHAKQTTGNHSREQTDEWDAERLSLRQELSSTQDSFKTTVARFESQLAKVQSDLASARAEAEIAVNDTKQAESAATQSRSDLESLRRQHAQLEERAREAESRVQMFLDQFETSVDNYRRQSQLPADALNGAHGRHHTHDSVASAESLYSHNDDSSTPEATVRPTSAATRNSMALDNLASELDALRSHWETTNKAYRLSDRFDFERTPMSDNNDLSESLSQWRSRANSENEKQRNDTTSARPDISSVSSTAPPVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPE3</accession>
    <name>A0A9W9CPE3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003403</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPE3"/>
    <dbReference type="OrthoDB" id="3641682at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="186" mass="19897" checksum="789426D3454CB6E0" modified="2023-11-08" version="1">MRSVIFALAATAMAADVSVVWRHEKSSGSTSLAIHSADDHSVLAESCGDHIGTVDFSNVDENGAGNFSVEGNTFAVSSKNQDGVSCTRIYNGAVAVVNCSPVDFDVPEGTPKSADCFTDDEAKASFVALKARHVDDMVSATRVEQRDSPSKTFRPRQQCYDETGTAQIGDGDPHQNYYHKQISVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPW2</accession>
    <name>A0A9W9CPW2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Complex I intermediate-associated protein 84, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_002254</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPW2"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050667">
        <property type="entry name" value="PPR-containing_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939">
        <property type="entry name" value="MEMBRANE-ASSOCIATED SALT-INDUCIBLE PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939:SF5">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4375170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="815" mass="93726" checksum="2E61D472FDDD8FA6" modified="2023-11-08" version="1">MPSLLTRVVFRSIIANEPLLYRGCRYQSPRPRILPHHGARSFPPVQRRTFLDLFKPQRKLKPMEMPAGLEKMIELTYAQHNALRPPKPTEVAEAFKAFFEQRKGAYEDYHIATAHRAFLYLLENPQGDEQPWLTKEELKRVFGKMIHPSRRPEGNGQAHLAFGRAILDEIANMVEHKEMNDDVESPKKDRRLEDTYTEVNSIHLLSLFGAAIEARDMLIDKLQYDPNTTAQQRATTLSAWKSVLGGLIQEGHPDEVLKWTDLLQEYSIPFTRQMQHKLVVFFAEHKDYERAKFWYSRPVVTRWGDKSAQPMGGTSSALLKACASNGDLSFGHQVVATILKDDMPNKESWDAIFLWSAAIGKGVDEIDRMMNVLIRRNDEARQKNPAVEVIRPDIETINALVEVSMAKQDPYSAERYIALGEKRGIWPNEKTYTMQIQYRISVGDIDGARAAYFNLQGNISGAEQTVGVINQLIQVLCISKQHHFDELMTMVDDLHERKAHFAAETVAALTLLHLRRGEIHDAMDLLQVHAHQYSPTQRVVIQKALSGFILDGETSTADAWDGYQILRNVFPETPREVRIPIMNEFFARKRSDMGCHVFFHMRDHPVDTQRANQDVYAAAFVGFARCADAESLELAHNQLKLDVKVDMDTRLRNSLMLAYAATGENLKALRFWREICESKEGPSYNSIAIAFRSCEGMHLGGEHARSIWKRLKEQDVEIDKTIWTAYMAAIARNHHHDEAQALIETVEEEYSFTPDLHILGSWFNMTVNSDKQEHVEEWIKQRYPEVWKEMEGLGYWVTMEGFGYRQYNINRDLDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQH0</accession>
    <name>A0A9W9CQH0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001967</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQH0"/>
    <dbReference type="OrthoDB" id="3798432at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="304"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="390"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="445"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="317"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="392"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="447"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="696" mass="78024" checksum="9DDA75C8C454A215" modified="2023-11-08" version="1">MDIRGQAGHPEQQTTHGNHSPLSIQKKLRYVSEAQWELAKVRKQLLVKRQRVRTFSRNVQLKRVDAGDAEATFMSRLRAFINENRDKLPTILIDAYDKVEETRNDLGETEENYLQAERDLTGAEWTFIDQENDFYQYGLQDLLEGEPGVNPMPPQNDQHIHNNPPPPPPPPFPVSVGTFPSASMFSVPSFPPPPPPPPVAPGQNRLRSADLPSDGLVNRDYHTVVAELETLRKRFNGLRQEQKQRITEAEANNVTIDEYTLIKPESPSFTDDYMRILEGIARREVEVQRLRLGDEQELQALAAERRHSEPTHCFKTPPSTVSMKRSQTESGAPPISKELTTKTKVQKWLLQDLKHNAVQKALYLTTLESLGVSSAADGAWEDLATEYWSQDSSRDYGDDSNVEKTVTHDTSHEPDLEKATTEFPYATPSLKALLRLDISRDRDPSEACSSCKDSFQSDFDGPSCTPLPPSPEERTTIVRDDEPLVPYLVLTPSDATEQIPYGQLPSTDMPLQQQPNLQTTDVVTLPGRKDSGQSMGIPGIEVCHVGLVEGEQYDPSSSHNHNAPQTPMQNNPYQVTKGSKEAITIVTTGLGPSSSATMQSAIRVDHFKPPSENYHATRSQSPNLSPCESPRLHGISITVTPPSRSNSQHRVKGTRKRIRDLVARTHHGRSLSTSFLLGEHTISKFKEQYEERHLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQQ0</accession>
    <name>A0A9W9CQQ0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translation initiation inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_002334</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQQ0"/>
    <dbReference type="OrthoDB" id="309640at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00448">
        <property type="entry name" value="YjgF_YER057c_UK114_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.40:FF:000004">
        <property type="entry name" value="Translation initiation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="2-IMINOBUTANOATE/2-IMINOPROPANOATE DEAMINASE RIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803:SF12">
        <property type="entry name" value="TRANSLATION INITIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4375248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="12677" checksum="480ED5578672EBFC" modified="2023-11-08" version="1">MSRQLISSEKFPPKPHNCPAVKVPGLVFLAGQTATGEIKQATRTVLQNLKEVLELSGSSLEQVVKYNVYLADMKDFAAMNEVYIEFLPKPMPSRSCLQALPPGDGTVIEIECIAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQW6</accession>
    <name>A0A9W9CQW6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_000200</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Component of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3647690at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038609">
        <property type="entry name" value="HDA1_su2/3_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="4">
        <location>
            <begin position="777"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="360"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="741"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="831"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="942"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="997"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1053"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1151"/>
            <end position="1504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1546"/>
            <end position="1573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2154"/>
            <end position="2199"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1940"/>
            <end position="1967"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1996"/>
            <end position="2069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="360"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="412"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="424"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="436"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="463"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="486"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="512"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="528"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="574"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="593"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="649"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="760"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="849"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="896"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="942"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="952"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="997"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1011"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1054"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1169"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1208"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1242"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1278"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1314"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1336"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1349"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1410"/>
            <end position="1419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1428"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1452"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1558"/>
            <end position="1572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4377375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="2199" mass="244589" checksum="209422DE5541DD29" modified="2023-11-08" version="1">MAQSDEAKHANTDLIIAQLKFKQALKTEDIEARLPPVSVELSAQFFSALDAVLEKNTSIHVQKCTEWIVKHITGSKTRTLVLGGYLVSVSKSVIVVQQLAGKAPSAAPTPVKKAIRNCIDLLLIVNDVLHADKFHRRSNSNHGTFGTECADSIRELVELAASCIREKNTQAEKKLRALLKFWALNQLVSTQDIEVLQKRADEALLLAQGGVRKRHYLLPEYHGDPSAPWHELPASYMLEPLIKYPQRSINPSQIKIAKFGKKEPSPHVRNLLDQFFEKIDLECVPTGKTQTDSDDKEDVPKFPVQTDKYDMWLDPMAQTVKQIKGTEDRAIVSTTYGWSSKFCEDMREHNVPENIKIAREDFQRMGEDEEVREPPQRRREEEGNSSRGRRHEEDDYPNQPRHRRRRSSSGSDYRREHSRDRRGSSVSYDSPRSRSVSRDRSYDRHDRQPKDERRDSDDRRDNYVNNASGQSRRPSQFRGKGSSYSKSRRRGKNRTNRNAGSSGRNYYAVPPQMSQAPQNFGEGSSQAPNPPFPVPPFAPQVGLPGQFPGSFPIGQFPSNLQFPPPLQSQGPGGFMPPPLPPNFSGPFPPPMPNMTAMPSNSYNRGNQFGNHQGNQYGNHQGNQQGNQYANQQGNHHQGNLQGHNQGYDNNGGYGQNSGGFGGGRGGYGPRGNYQRGGYNNRGGYGASQRGPRNCSPRIVPRPLSYCYHAVNHNSTFRTLPIDHGYNLELYNNSQRFCRVMALPKRTRNSKRRESSKRPEYTTSSDSEHSQTASSEWWEADCILEEKVRGRKRQYLIKWKGIDPATGQEYPPNWETEDCANELLVESWEQEKANKGVAGPAGNTGSGSKGKGHLRRQQHALRTPRRNRKSRAVQSSPEPAARSPLLPTVSSTPARDSTTPTVGHSTVTTPVSAPKCSSQRASPRIQIIQRRNSFNREEYDRFSQIAASQQASTQPRTQETDLNSSQLFDAARPFSLHIVPDSQSSAGEGSFIPITQQTGFSSQQSSDIDASQDEKEATQDSVRLHDSHLHIDPITNKELQGIFEIVQAASRAASPATSIPETVPETTADSQSQHRRIDTEDQRVISETLDTFEGASQAAQAVAEDGGPDIDQEYAEQITAEGESERRQEESITQEYDTFPDSVGGAVEEAQVLPGDNPIVIDIGTQSRPPVEDQRTSDRAKEPADDKAHEISQPQSSQQIGPWDSQKSLAKEGNFHLRGSTDVDLGANPVPQTQPAEILVQGSIDSVTVPESSENTPQEPELASTTHNSLSAVIDQPQLDGQQGDSTAEFNHIPQFDTEEAIRRRDFALSPHAPRSSEHSTESREQNAQVLPGEPDFSTQEETTQSIRPSVEEDYIHNRAGSESRHDSSQETPERQLKSVENSSSPIPQPPSQSLDTVASKLPSRPVTPGLITSSSTMASHDTGDDVAQELKERLAKRQAENPFTPKRRIFRSSFTPSATALNPESSAAAPTSAGRFLEANAAEGTRSPSAVPDRSPASQAPTSLRTVAFASNSTVPVAEQHEKEMASSASIAPQQVDINVMVTGEESAMPSHVPTSTEPEDEELSDINDDSESVLNDDLSLEREEYIVPLFIEGRQCDMYTEHLKYKKELLEAFLADTNNFDQLAQVEEVLSHLRALETHIDLVFAEAEPASWVGTNPGTQVEFAAQFGMENSVKFRFLHTLFHHLRDKDKHIVLVTAADNGALFDILETFCRAKFINYSVPTRGRQAIPQNVEGALTVTILPGDSAPVIRSPDLIVCLDGFQEAAQIRQMNWARSSGKDIVPVLHLVIPRSVGHIERYVSPALGHKERLHTILASLAQMHGDIGKPMDEDSPRAPIAAEMVVDWFVAANDDEGLSWPLPSIGSVKNVIEYQTQMSQASHTSAISPVPERNKRPLDDEALDPAKRMRFTPQPQGVPGSNVNHEPGVTHISDSMPGTAANVASLRRQLAQIEEAYRKERDARKAEEQRFSDHEEMWDKQQTVHENLTREYRLLLGQQKSTEEKLETMTTNNETLRNRLADRTTEMKQLNDQLDEQRATHLLSEDEKIAEITRLRQELTTTQAERQKALRNVQTTESTLEYTKEQYRLAQDTATSSQSLMEELTASNAKLQHQASGQPAKLKALHLDRQAENQDRQIKSLRAENLILKRTLGQKEEELQRLRSNPGRAGVGTRAQSVTPQPKVRSRAASPMGGRLSNLRNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQX7</accession>
    <name>A0A9W9CQX7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Vacuolar transporter chaperone complex subunit 4</fullName>
            <ecNumber evidence="3">2.7.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Polyphosphate kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">SPX-dependent polyphosphate polymerase VTC subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Vacuolar membrane polyphosphate polymerase catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">VTC4</name>
        <name evidence="18" type="ORF">N0V83_001096</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[phosphate](n) + ATP = [phosphate](n+1) + ADP</text>
            <dbReference type="Rhea" id="RHEA:19573"/>
            <dbReference type="Rhea" id="RHEA-COMP:9859"/>
            <dbReference type="Rhea" id="RHEA-COMP:14280"/>
            <dbReference type="ChEBI" id="CHEBI:16838"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19574"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Vacuole membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the VTC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.1" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQX7"/>
    <dbReference type="OrthoDB" id="6493944at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033254">
        <property type="term" value="C:vacuolar transporter chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008976">
        <property type="term" value="F:polyphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016237">
        <property type="term" value="P:microautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07751">
        <property type="entry name" value="PolyPPase_VTC4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14480">
        <property type="entry name" value="SPX_VTC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.100.30:FF:000001">
        <property type="entry name" value="Vacuolar transporter chaperone 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.30">
        <property type="entry name" value="VTC, catalytic tunnel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018966">
        <property type="entry name" value="VTC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042267">
        <property type="entry name" value="VTC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF1">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE COMPLEX SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09359">
        <property type="entry name" value="VTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="712"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="758"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="17">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="492"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="510"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="533"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4375819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="805" mass="92652" checksum="10D8F20F8A95E454" modified="2023-11-08" version="1">MRFGQQLKQSLNKEWIFYYIDYEGLKDALRVHHIWDEKSEQSFVEQLEKELDKVYTFQRVKSEEIIRRIAASDKEVNGAVSRAQQAPGQTSDSFEDDFDLLEEDLSDIIADVHDLAKFTQLNYTGFQKIIKKHDKNTSWVLKPVFAARLKRKPFFQDNYDAYVVKLSRLYDIVRTRGNPITGDSSAGGKQQNFVRQTTKYWVHPDNILELKLIVLKHLPVLVFNPNKEFNEEDSAITSIYFDNTETWELYMGRLKKTEGAEAIRLRWYGGMSSDTIFIERKTHREDWTGEESVKARFKLKEKNVNAYLRGELRPEQIFEKERREQKRPEKDIASDEQLAREIQYRVLTRKLEPVTRSFYHRTAFQLPGDARVRISLDTELTMTREDNLDGKKRAGDDWRRMDIGVDWPFKQLPKEDKELFPYAVLEVKLQTQAGQEPPQWIRDLTSSHLVEAVPKFSKYIHGTATLNPKRIDLIPYWAPQMDVDIRKPVSHKFGIDRPGASNDISTSGNLDDDSEDDDAATELPAANGSEDDDRLRRLREARDVMEQHEMDRTRDYGTLTNADDPNALDIEERVAAGRNRDNDYPIYDSDSDDEDEDYEVAKREGGLKFYRKWVERKATTVGTTVLHGFISIIPKPTPTDVGDGGFTLGVPTWKQAGEVKRFKAPKGKRIHVPVRVEPKVQLATERTFLSWLEFSILIGGIAATLLNFGDGLAFAAAWAFTIIACLALLYSAGLYLWRVKKIKERRAVVYHDKWGPTLLCLGLFAAVAVSFGYRFGKGDMTGFEGGLRARNNGEMIVVKLWAGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR54</accession>
    <name>A0A9W9CR54_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial distribution and morphology protein 34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">MDM34</name>
        <name evidence="13" type="ORF">N0V83_001203</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the ERMES/MDM complex, which serves as a molecular tether to connect the endoplasmic reticulum (ER) and mitochondria. Components of this complex are involved in the control of mitochondrial shape and protein biogenesis, and function in nonvesicular lipid trafficking between the ER and mitochondria. MDM34 is required for the interaction of the ER-resident membrane protein MMM1 and the outer mitochondrial membrane-resident beta-barrel protein MDM10.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the ER-mitochondria encounter structure (ERMES) or MDM complex, composed of MMM1, MDM10, MDM12 and MDM34.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Mitochondrion outer membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="10">The ERMES/MDM complex localizes to a few discrete foci (around 10 per single cell), that represent mitochondria-endoplasmic reticulum junctions. These foci are often found next to mtDNA nucleoids.</text>
    </comment>
    <comment type="domain">
        <text evidence="10">Lacks alpha-helical transmembrane segments, suggesting that it resides in the membrane via beta-sheet conformations similar to those predicted for other outer membrane proteins and porin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the MDM34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR54"/>
    <dbReference type="OrthoDB" id="17927at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21673">
        <property type="entry name" value="SMP_Mdm34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03105">
        <property type="entry name" value="Mdm34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027536">
        <property type="entry name" value="MDM34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058825">
        <property type="entry name" value="MDM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28185">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28185:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26545">
        <property type="entry name" value="Mdm34_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="12">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="321"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="542"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="321"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="332"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="372"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="433"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="448"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="482"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="542"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4375925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="592" mass="64207" checksum="1813B94B7EAFC7A2" modified="2023-11-08" version="1">MAFNFNWSPLIADTSRARDMLTTALNKSPKPPIIVDDIIVTELNLGTTPPELEILEIGDLAEDRFRGIFKMSYAGDAFLTLKTKVQANPLNTYLSTKPSFASPQPLAASSGLTIPLQITLSDIRLSGFVILVFSKQKGLTLVFRNDPLESLKVSSTFDSIPFVRDYLQKEIEGQLRVLFMEDLPAIIHRLSLRLWSPEFEELETEERLEGSHDTTAPIDPLASPPQDAVDAFGNALDESQISALSLDSPSEIHASFSQKNILRLAALSESQRTLSLFTPGIRDAVFRAWAGHSDKPDSGAATPALTQGSLSRIQSTFGSLKSTSSSVASGSTGNETYSSRPTLASSHSMPAGLSLGANRTRAGGMRKRKKRVVDLRKKDGAESSGLSTEANTPLPSTYVSETSSVIHEEREAEEELVTPPRSPANPSFFSEGRNGRLDADEPSRPSESTPTTAPLLAPAPLLSSPPKSSNPKQTTTPISRRPTLSRSELRKVQQSTSPLLRSLSFDKVPSLASPPYTDNTSSSSGGILEQAWMLKMAQEIARKVQEEKDRTDRGSPSRPSQRSRRRTEKAAASGGMWASAEDEIDAPPAYVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR90</accession>
    <name>A0A9W9CR90_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_001445</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR90"/>
    <dbReference type="OrthoDB" id="248233at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04165">
        <property type="entry name" value="GTPBP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03694">
        <property type="entry name" value="GTPBP_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03708">
        <property type="entry name" value="GTPBP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000014">
        <property type="entry name" value="Probable GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000091">
        <property type="entry name" value="Probable GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035531">
        <property type="entry name" value="GTPBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF9">
        <property type="entry name" value="GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="5">
        <location>
            <begin position="177"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="601"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="616"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4376164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="633" mass="69901" checksum="27F6FA54A2C77C64" modified="2023-11-08" version="1">MASRHTAKQNPLERRKGESALSEFADYVQKQQALRRPPGQAPAVLEDHDELSILDELGLSDDAKSHIRLKTLLIDPAEENIAALTEHIKDRLAEGHGEVLFDVGLEDSGDSMGFSKENWDFALDRIGVVCEQIKADYKLLMTRNVDSNVEVGPRDAKDTGASGKMILRQRPENVDDVIETRIAVVGNVDAGKSTMLGVLVKGGLDDGRGKARVNLFRHKHEIESGRTSSVGMEIMGFDSKSEVVVSNVAGRKLTWEEIGRRSAKVISFTDLAGHERYLRTTVFGLLSSEPNYCLLMVAANNGLVGMSKEHLGIALALNVPVMVVITKIDICPPQILEQTITQLTKILKSPGARKIPIFIKNREECINTATQFVSRRICPIFQVSNVTGHNLDLVRMFLNVLPHHGNYDSSAPLEFHVNDTFSVPFVGTVVSGVVKSGIIHSGDTVLIGPDSLGQFQTTKVRSIERKRIQVPGCSAGQSASLALRNVRRKDVRKGMVVLHKADKPDPTTGVIPNPKVYREFVAEVLILSHATTIKTRYQAMLHVGPVSQTCAIIDIDRQYIRTGDRAQVAFRFVQRPEYLTVGDRILFREGRTKGLGIVKRVGYDPKHPLNPELQQEQDKGKDNQEDGKKVEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSA8</accession>
    <name>A0A9W9CSA8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Arf GTPase arl1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ARL1</name>
        <name evidence="6" type="ORF">N0V83_000732</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSA8"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04151">
        <property type="entry name" value="Arl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000624">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4377902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="182" mass="20098" checksum="65494FB7C6085862" modified="2023-11-08" version="1">MGGYLSTFTKLIWAKKEIRILILGLDNAGKTTLLYRLKIGEVVTTIPTIGFNVESVTYKNLNFNVWDLGGQTSIRPYWRCYYANTAAVIFVIDSTDIERLTTASEELRAMLNEEELRDAALLVFANKQDQPGAKGAGEISEALKLGELKDRNWSIVACSAVDGRGVQEGMDWLVSNVGDGQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XWX9</accession>
    <name>A0A9W8XWX9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tat pathway signal sequence domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_010775</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XWX9"/>
    <dbReference type="OrthoDB" id="4798501at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045793">
        <property type="entry name" value="PcRGLX/YetA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048330">
        <property type="entry name" value="PcRGLX/YetA_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048331">
        <property type="entry name" value="PcRGLX/YetA_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048329">
        <property type="entry name" value="PcRGLX_1st"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40081">
        <property type="entry name" value="CONCANAVALIN A-LIKE LECTIN/GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40081:SF1">
        <property type="entry name" value="TAT PATHWAY SIGNAL SEQUENCE DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19501">
        <property type="entry name" value="PcRGLX_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21345">
        <property type="entry name" value="PcRGLX_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21346">
        <property type="entry name" value="PcRGLX_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040821061" description="Tat pathway signal sequence domain protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="domain" description="PcRGLX/YetA-like N-terminal RIFT barrel" evidence="2">
        <location>
            <begin position="21"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="PcRGLX/YetA-like central beta-sandwich" evidence="3">
        <location>
            <begin position="113"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="PcRGLX/YetA-like C-terminal alpha/alpha toroid" evidence="4">
        <location>
            <begin position="485"/>
            <end position="911"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4361834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="926" mass="102161" checksum="E364597ABBBD7DE7" modified="2023-11-08" version="1">MLTTVLGVLGLISCATAQSSSVKVKWLDGTPKYLSGVTFGLPWPRGQHLANATTFTASGDAQLQSWATAYWPDGSLKWTGHALAASDAPAEEYIITASGPGISTRSRRQSNGVKVSDTDNDIVVNTGKITATFPKSGNTLVSSIETGNKVVGINGRLILRSQTGTPDDDEDGKPVALDHFTFESKVSNVTVSRDTIARTLVTVSGVHTIDGDGSHADWFPFTVRFYLYANSEAIRIVHTIIYDGDSTKDFISGIGIRFDVPLAGEEFYNRHIRIAGVDGGLLHEAVQGITGLRRDPGIDVRSGQYNGTLLPNASTWDQKVTSRLQWIPTWSDFRLTQLSPDGFNLKKRTKTGQSWVKIPGGTRSGGLAYLGGATVGGLALGLRDFWKKYPTSLDISNGATDTGSITLWLYSPQAGPMDLRPYHDGLGEDTYAKQLDALEITYEDYEAGYNTPYGIGRTNELFLYAFHSTPEADKLSLLTNYTNNPPVLFPEPEYVRDTKAIGGYWDVPSAVNSTAARTIESNMDFLIKFYESQVEERRWYGFWDHGDVIHTYDDDRHQWRYDIGGYAWDNSELSPDLFFWNFFLRTGRADAYRLAHDQVRHGGEVDSYHLGNFTGLGTRHGVQHWGDSAKQARIATPVYRKTFYYISGGDERTGDLVHEVLDVDKAFVLVDARRKVRDPSVVYTPDPKALYLNFGTDWAGLAQAYLIEWERRGPRWEEARDKLLESVKTYPRLKNGFVTGEALYNSLTGAWSPPPTDPSNSGNITVSHLSGVFGVLETIDQLIEHFGPDAQNATKPFYDAFLDYCYYYGASKAEQKARYGADFGSLSLKQGHSRYTAYVAYHRNNATLVPRVWTEFLGDGSKDGLAPTAPWKTERISGSDVLIPVDEAIWVSTNAAALYGVAGIESLALVGAPDVNRIAGNTTTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXN0</accession>
    <name>A0A9W8XXN0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glutaredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TTR1</name>
        <name evidence="9" type="ORF">N0V83_011020</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1-chloro-2,4-dinitrobenzene + glutathione = 2,4-dinitrophenyl-S-glutathione + chloride + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51220"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:34718"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:133977"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 glutathione + H2O2 = glutathione disulfide + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:16833"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="EC" id="1.11.1.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 11">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXN0"/>
    <dbReference type="OrthoDB" id="418495at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015038">
        <property type="term" value="F:glutathione disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000026">
        <property type="entry name" value="Glutaredoxin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011767">
        <property type="entry name" value="GLR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011899">
        <property type="entry name" value="Glutaredoxin_euk/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02180">
        <property type="entry name" value="GRX_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694:SF18">
        <property type="entry name" value="GLUTAREDOXIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00195">
        <property type="entry name" value="GLUTAREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="8">
        <location>
            <begin position="17"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4362078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="102" mass="10877" checksum="13C18EDFDA02103D" modified="2023-11-08" version="1">MSATKTKVQSIIDENPVAVFSKSYCPYCRAAKQLLSESGAKFYAIELDQVDDGSAIQSTLGELTGQTTVPNIFIAQKHVGGNSDLQSKKKDLPSLLKDAGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYT9</accession>
    <name>A0A9W8XYT9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYT9"/>
    <dbReference type="OrthoDB" id="3770142at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040906253" evidence="1">
        <location>
            <begin position="20"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="151" mass="16047" checksum="B210D7B47FD23D54" modified="2023-11-08" version="1">MRTASILALATGLIAPAVADFYVYASVQYITADASNTNDFTFFAGPPSCDDVYNSIGLSPTDDASGGGVRCKGCSTAENNDVKVTELEFKANDYGHYTWYAERDGAPLVDVNDAVVGTCNIDNSDNYNCFDGLATYKGQSQIFCSTSLAIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZN6</accession>
    <name>A0A9W8XZN6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="19">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">DRS2</name>
        <name evidence="23" type="ORF">N0V83_009753</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 19">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38567"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:38568"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Membrane</location>
            <topology evidence="19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="19"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000026">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000047">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000010">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF150">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 18 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="491"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="539"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1092"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1135"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1171"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1200"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1245"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="21">
        <location>
            <begin position="226"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="22">
        <location>
            <begin position="1021"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="122"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="177"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="20">
        <location>
            <begin position="63"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="16">
        <location>
            <position position="603"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="603"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="603"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="604"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="605"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="605"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="697"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="738"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="761"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="795"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="876"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="877"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="878"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="969"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="975"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="995"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="998"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="999"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="999"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ4363460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1348" mass="151251" checksum="5F76D8B0BFC61E33" modified="2023-11-08" version="1">MAGRPPGAPPHSDNLIDFDDHQPTYYSGARPPANDDDLLHRYDIDSSDAAPQGRPSVSYDDFVGGGRPTAGLPGGPGAPAAGPPYVHDRAYSQTSDLNNYQRYSDAEIPADDDHSVQGYYAAGGAYDQSSPGIQRGPSKKAHNRNSILSLGGGLTGRMKNMLGRGSEYSEMDLPLTEHAAHQSQPSASTYDDGASSKKTKKRSSGTFKFGFGRGAPDPSTLGPRIIHLNNPPANSQNKYVDNHISTAKYNVFTFLPKFLYEQFSKYANLFFLFTAILQQIPGISPTSKYTTIVPLCIVLLVSAVKEYIEDYRRKQSDSQLNNSKAQVLKGSTFADTKWVNVAVGDIIRVESEQPFPTDLVLLASSEPEGLCYIETANLDGETNLKIKQAIPETADYVSPAELARLGGRIRSEQPNSSLYTYEATLTIAAGGGEKELPLAPDQLLLRGATLRNTPWIHGVVVFTGHETKLMRNATATPIKTTAVERLVNKQILMLVVILVCLSIVSSIGDVIIQSRQRAQLEYLDLDAFNGAKQFFRDLLTYWVLYSNLVPISLFVTIEIVKYYTGSLIDSDLDIYYEPTDTPANCRTSSLVEELGQIEYIFSDKTGTLTCNMMEFRQSSIAGIQYADEVPEDRRATVEDGLEVGIHDFKQLEQNRQSHSNKYIIDQFLTLLSTCHTVIPERGGEKDAIKYQAASPDEGALVEGAVTLGYKFVARKPRAVIIEVDGRRLEYELLAVCEFNSTRKRMSTIFRTPEGKIVCYCKGADTVILERLAKENNPHVETTLSHLEEYASEGLRTLCLAMREITENEFQEWWSIYNTAQTTVSGNRAEELDKAAELIEHDLTLLGATAIEDKLQDGVPDTIATLQSAGIKVWVLTGDRQETAINIGMSCKLISEDMSLLIINEENKEDTRDNIRKKYQAVTSQSQGGAEMDVLALVIDGKSLTYALERDLEKEFLDLAVRCKAVICCRVSPLQKALVVKLVKRHLKSILLAIGDGANDVSMIQAAHVGVGISGVEGLQAARSADISIGQFRYLRKLLLVHGAWSYQRISKVILYSFYKNIAMFMTQFWYSFQNGFSGQIIYESWTLTFYNVFFTAAPPFVLGIFDQFISARLLDRYPQLYRLSQSGVFFRMHSFWSWVGNGFYHSLILYFGSQAFILWDWPQWDGRNAGHWVWGTASYTANLATVLLKASLITNIWTKYTFLAIPGSFLLWFILMPIYATVAPKVNISNEYIGVIERLFPDPRFWAMVVVLPPLCLIRDFAWKYAKRMYFPQSYHHVQEIQKYNIQDYRPRMEQFQKAIRKVRQVQRMRKQRGYAFSQTDESQARVLQAYDTTQQRGRYGEMASSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZQ1</accession>
    <name>A0A9W8XZQ1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Purine permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_010333</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZQ1"/>
    <dbReference type="OrthoDB" id="1641903at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042907">
        <property type="term" value="F:xanthine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42810">
        <property type="entry name" value="PURINE PERMEASE C1399.01C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42810:SF2">
        <property type="entry name" value="PURINE PERMEASE C1399.01C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="349"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4363213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="463" mass="49270" checksum="294FA04AD396091B" modified="2023-11-08" version="1">MQAMETSKSPKEDNKLVEDTPTTSTYEAAAPGTISKVSFHDKATQLAAYLTSRDAWLGNYDYWYLVTPNIYPFNKKYKEKAIPFYGLHDEVPILLTIILGLQHALIMIGSIVSPPLAIASGAFYLDSAQVSYLVSAAFITTGIATAIQVTRLHIKGTPFFVGTGLLSVVGPTFDIIPIAFSYTSMRYKNGTCPVAPDGTQLPCPDAWGAFLGTMLCTVLIQIAMSLVPPRFLNKLFPKIVTGTLLLLVGVYLIGNGMQNWAGSSNCNGGDGFYALCPNIAAPNPLPWAHPKLIGLGFSVFFTIVVVEQFGAPIMKSAAVLFGLAVGCAISGATGYWSRENIDSAPVVTFLWVHTFKLSVDGALVLPLLIMFICQGVSCMPDILATAEISGVDIEGTQFNSRIQGGILCDGLGSLFSALGTAGPMVSQAGNNGVIILTGCAVSWTLSPPFVAWFCSALSCQLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZR5</accession>
    <name>A0A9W8XZR5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZR5"/>
    <dbReference type="OrthoDB" id="5291209at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040894474" description="Peptidase A1 domain-containing protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="393" mass="42849" checksum="2DEBD8E803D3669F" modified="2023-11-08" version="1">MLWNTLLLFLTPALARTRLPARDTDPNDLSIYKQAIYLPFTKDFSSDYTPTVRGSINGRSFTFPVDTGSTGLMIGAPLLPDVDLSGSNADQYEQGWEFLTSSSLLYNGRFVPLDVTFYGSSRKYTAVSKVLVLVVQSVIKCPAYNSTTGQGICPDGGSVQSTASPKHVTYMGVGFGRNVAGSGLNFGSSDHNPFLNIVNINGRSTYSFRTGYVVSTTGVHLGLTTRNTRDAVWVDLDYGATDDWRDWAMVDMAMRVNEGQDEYAGKALVDTGIPQMFIQPPPGVKLPTVRILNPATNPTTKYTSRITNGTYLDFAFPSFQDGVAGYDFEVGDESFPSQPAYVQPVAGRPSTYFNSGRNFLFGFTIIFDADGGRFGLICLKCNDDEGYYGSGTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y014</accession>
    <name>A0A9W8Y014_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Triosephosphate isomerase</fullName>
            <ecNumber evidence="6 11">5.3.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TPI1</name>
        <name evidence="12" type="ORF">N0V83_010669</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="6 11"/>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y014"/>
    <dbReference type="OrthoDB" id="6715177at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046166">
        <property type="term" value="P:glyceraldehyde-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000025">
        <property type="entry name" value="Triosephosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_B">
        <property type="entry name" value="TIM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022896">
        <property type="entry name" value="TrioseP_Isoase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139:SF41">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="Triosephosphate isomerase (TIM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="9 11" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 11 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4362575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="248" mass="26913" checksum="0AEF411A823C7CCE" modified="2023-11-08" version="1">MGRQFFVGGNFKMNGNIKSIKDIVKNINDAKLDSNVEVVIAPPALYLLLTREHLRQGIEVAAQNIFDKPNGAFTGEISADQLKDSGITWTILGHSERRVILQEDDSFVASKTKAAIDAGLSVILCCGESLEQREKGETVSVVTKQLKAVADKVKDWTKIVVAYEPIWAIGTGKVATTEQAQEVHAAIREWLKKEVSSEAAEKTRILYGGSVTEKNSAELAKQADIDGFLVGGASLKPAFVDIINSQQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y052</accession>
    <name>A0A9W8Y052_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cu/Pi carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PIC2</name>
        <name evidence="13" type="ORF">N0V83_009883</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Transport of phosphate groups from the cytosol to the mitochondrial matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y052"/>
    <dbReference type="OrthoDB" id="427452at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035434">
        <property type="term" value="P:copper ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000076">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000131">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF13">
        <property type="entry name" value="PHOSPHATE TRANSPORTER PIC2, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G12080)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="17"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="111"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="212"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4363587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="305" mass="33450" checksum="36E76A53F82F06DC" modified="2023-11-08" version="1">MANKMDAKKKIELFSPSYFAACTIGGIIACGPTHTMVTPLDLVKCRRQVDSSIYKSNTQAWKMIYSKEGLRGVFFGWSPTFIGYSMQGAGKYGFYEGFKYLYGDKLAPGMPKQVVYLAASASAEFLADIALCPMEAIKVRMQTTLPPFAHTLREGWAKVIREEGVGGLYKGLYPLWARQIPYTMVKFATFEETVSQIYKFLGKPKDQFSSLAQTGVSFLGGYIAGVGCAVVSHPADVMVSKLNSDRKAGEGAGQAIGRIYSKIGFGGLWNGLPVRIFMIGTLTAFQWLIYDSFKVYLGLPTTGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y083</accession>
    <name>A0A9W8Y083_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y083"/>
    <dbReference type="OrthoDB" id="7777654at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006598">
        <property type="term" value="P:polyamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF313">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="502" mass="56082" checksum="A74DF29F45316FFA" modified="2023-11-08" version="1">MAVIGAGVAGITAAQALANQSITDFVIVEYNDVIGGRMRNTKFGSDSDGNPYVVELGANWISGLGNDTLDGPENPVWTFSKQVNLSSANSDASSIVTYNETGAVDYTDILDEFEEYWAIFEQNAGTILTENLQDRSFRAGLWQSGWRPKGDAARKAVEWWMWDWETAQTPEESSFVYGITGYNLTYYAFSELSYFSVDQRGFNTWIKGEAAKFLEANDTRLHFNTVVTDIEYSDSGVKITNEDGSCVEADYAINTVSLGVLQNDAIAFEPELPDWKQDSIATFTMATYTKMFFQFNETFWPTDKQFFLYADPTTRGYYTVWQSLSTEGFLPGSNILFATLVDEQSYRVEAQDDETTKAEAMVVLRQMFPDIDVPEPIAFTYPRWTSTPWSYGSYSNWPTGTTLEMHQNLRANVGRLYFAGEATSAEYFGFLHGAWFEGREAGERIAGMVTTECRNVESGCGNYTSYEVLHGTTDFSEVNAFNGMGVSPLFVADTGDEEESDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0W2</accession>
    <name>A0A9W8Y0W2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF3669 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008648</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0W2"/>
    <dbReference type="OrthoDB" id="2993351at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022137">
        <property type="entry name" value="Znf_prot_DUF3669"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40780">
        <property type="entry name" value="DUF3669 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40780:SF2">
        <property type="entry name" value="DUF3669 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12417">
        <property type="entry name" value="DUF3669"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3669" evidence="1">
        <location>
            <begin position="27"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="110" mass="12934" checksum="D37C864DB4A814CE" modified="2023-11-08" version="1">MADIQNATKDTLAARYVSHLRHRTISMWLLDFSQCEKFSKDVSGLKKIVDVFWWNEPYYPRPSDKLWKVFVEHYLKTSFAFNKTTMPKAFIDQVVEVGKKRAVGDLFAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1F8</accession>
    <name>A0A9W8Y1F8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DASH complex subunit ASK1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">N0V83_009208</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex ASK1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1F8"/>
    <dbReference type="OrthoDB" id="5573898at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013964">
        <property type="entry name" value="DASH_Ask1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28200">
        <property type="entry name" value="DASH COMPLEX SUBUNIT ASK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28200:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT ASK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="18"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="187"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="150"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4364612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="347" mass="37684" checksum="917C4CEB6A1AAB1A" modified="2023-11-08" version="1">MQFWKQFFEASANVSLSGYEAPEQDESAMQEDTQQSQLYEDETVEDDTVTGSAATPPRSASAQDDLDSEITFDSPSLGHSTPRAPPSAKNGRTKNGTDGGEDVEPQFAGLSSPYEALKQEFREDIGGRKTPKLEPVTPGKTQALPDMTGFESSPFVQPTTSKKPYVNQDPLLHRVLDKTFRVQATPIISPRKYKPTGAFTPRAAHHGAPTPRGAASNLPRWAEDSSPPSSPAPQLRADIFSPMKTPRTPGVSVQTPAKGKTPMSIHPTGTVFDDSDEEYEIDLSPPKTMQFHIPQSKLLQTPAREASKRIVDDLLLTAGGDITDTTGGMDDDSPSVVRRQVDLDNSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2J2</accession>
    <name>A0A9W8Y2J2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_008574</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4364958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2J2"/>
    <dbReference type="OrthoDB" id="1431247at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="5">
        <location>
            <begin position="89"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="134"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="213"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="137"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4364958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="312" mass="34749" checksum="5C88C70D40C5D6DA" modified="2023-11-08" version="1">MAFVLTPRPVPAYQVPQCNPFGFCAPVSRPTYSYRVSRPQPRQPTYSSFNHFFSQVDELLGEIDRHAQHQEQLAAHLRAQREAHHQRQQRKRALRAKFAVSPTEQGWQIDGDIQGFEQDNISIEVTDEHTLKIAGNTRWQSEKVQPQTQLPETDASARADDVTQNGSENENALEPEATPVGVDSDTESHKSYQATVEDDFEDLGAEVASLISASSAPSTVGESTHPKGDETAARESTAAVTSKTPQPEHGEIALQQEKQPQEEDRVHGSFERTFRFPQRVNISNVSASFKDGGLKITVPRAQVLQTRRIVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2V5</accession>
    <name>A0A9W8Y2V5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008031</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2V5"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4366395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="793" mass="89519" checksum="8AD13E532D6A6EC5" modified="2023-11-08" version="1">MAIPLLGALVKAYKDNTDKVLQWIRKESTVHVLSEETNGNIRVDMKDLERAADDLRLRSVKPNADIKFAFEYALQLRELVTRSHEQEETKQYGHCLEQTIGHRVFNQRFRQVYNKICQAKPKDQRKLKKLTKKRMDPTEEFPAVHQVLHNIRGAGTSAEGLVSDTTDNKTMSMKESIDLIDLDIFAREALTLDAHIGAIKEYWERASECPLGPSLLLLGYLTRGASNYANERAGGSKLEHGMYQSTLVGALRHIRELTKTSGNEVPDHKPTIPNLIKYRLETGRSSYGYEGVRAEDMLPFMQTCLKSFLEEYRDNPTKISTQGPDHPTDAWFTNSARVAQNPSQTFDKTLLDKERTEAVIEICWNVVQATHRAGRFSENSAQKILADNLPLANVLARIVMPDSKKGAFDLEFSLNMTILLETTDSWLWSTPFLAQLKRNPRAIVEGFSDEVLVTERRFYMGNLAHLAEMLTCAPGQKAIRYIGLAKEMAKFYLHDSHRDLLHSLPWVCSSYLILMHNAVTVAGLALCNIKDQVAAVMYLYKALRSVQLVDSIWFMNYLIDRIGSVVFGPEETTFRGFHDTFTSRKDGNMIIDEGIFSWSRFPNLDYSYNKEFREVLIAGLPNDLASDDDKKRLLAGDCFLAGSGYMRGCFNLLWPHFDKNDLEGCGSFRALPGNPPRYEYTKPNDKAETLPTARLNFIAICTIFSKLVVSLNQATASPLSPESSAHSIKDGVKNIEQLLRQVDSVGAKEGHLMANVADNAQIHACLRLAADVLTKSTASYTENNMDSFEWRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y312</accession>
    <name>A0A9W8Y312_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome assembly protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_008141</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for efficient biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RSA3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y312"/>
    <dbReference type="OrthoDB" id="69550at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051898">
        <property type="entry name" value="Ribosome_Assembly_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028217">
        <property type="entry name" value="Rsa3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28127">
        <property type="entry name" value="RIBOSOME ASSEMBLY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28127:SF1">
        <property type="entry name" value="RIBOSOME ASSEMBLY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14615">
        <property type="entry name" value="Rsa3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Ribosome-assembly protein 3 C-terminal" evidence="9">
        <location>
            <begin position="118"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="54"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="87"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4365522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="178" mass="19378" checksum="9D68661278F6EA5B" modified="2023-11-08" version="1">MAGHKTPSGVEKKRKSKKRKSRLEVSSSSESDSDVAATKKAIPKAVEEEEEEPAQTPETPAGNSLSKSSKKEKKKNKAARDEEQGDSDTPMTDAIPTQSTTPKTSAKSTQLQQSQDNFTAIYLRKITTELADDLDKVREAQDFKASSVSMLIHALKQGESLFNVEEKRRVVGAAAAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y336</accession>
    <name>A0A9W8Y336_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">14-3-3 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">BMH1</name>
        <name evidence="6" type="ORF">N0V83_007858</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the 14-3-3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y336"/>
    <dbReference type="OrthoDB" id="10260625at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.190.20:FF:000002">
        <property type="entry name" value="14-3-3 protein epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023409">
        <property type="entry name" value="14-3-3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00796">
        <property type="entry name" value="1433_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00797">
        <property type="entry name" value="1433_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="5">
        <location>
            <begin position="4"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="233"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="252"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4366222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="265" mass="29385" checksum="C226E88FF3F2A634" modified="2023-11-08" version="1">MGYEDSVYLAKLAEQAERYEEMVENMKAVASADQELSVEERNLLSVAYKNVIGARRASWRIVTSIEQKEESKGNETQVTLIKEYRQKIEAELAKICEDILECLDGHLIPSAESGESKVFYHKMKGDYHRYLAEFAVGDKRKTSADKSLEAYKAATDVAASNLAPTHPIRLGLALNFSVFYYEILNSPDQACQLAKQAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSSEAEPSAEQPAAAEPATDAPKAESAEAPAETKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3U5</accession>
    <name>A0A9W8Y3U5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">bet5</name>
        <name evidence="8" type="ORF">N0V83_007681</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Part of the multisubunit transport protein particle (TRAPP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus</location>
            <location evidence="6">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAPP small subunits family. BET5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3U5"/>
    <dbReference type="OrthoDB" id="3364529at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14855">
        <property type="entry name" value="TRAPPC1_MUM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.70:FF:000013">
        <property type="entry name" value="TRAPP complex subunit, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007233">
        <property type="entry name" value="TRAPPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249:SF16">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04099">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01399">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="4 6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="23"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4367151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="170" mass="19296" checksum="DBECC9FB2979D4CE" modified="2023-11-08" version="1">MVLYSFYIFDRHTECIYARRWTPRPTSSSSKTARPQSGSSITSNGANDAVPNARKPMSHSDDEKLIFGLVFSLRNLVTKLGGPDDTFLSYRTGEYKLHYYETPTRMKFVMLTDTKVINLRQYLHQIWANLYVEYVVKNPLAPTEHPGGIGVANELFERGLEAFITAVLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3X8</accession>
    <name>A0A9W8Y3X8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Trafficking protein particle complex II-specific subunit 65 IgD3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006888</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3X8"/>
    <dbReference type="OrthoDB" id="5345392at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055420">
        <property type="entry name" value="IgD3_Trs65"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024662">
        <property type="entry name" value="Trs65"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28159">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX II-SPECIFIC SUBUNIT 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28159:SF1">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX II-SPECIFIC SUBUNIT 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12735">
        <property type="entry name" value="IgD3_Trs65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trafficking protein particle complex II-specific subunit 65 IgD3" evidence="2">
        <location>
            <begin position="417"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="594" mass="65632" checksum="85E71F8C01A79BB0" modified="2023-11-08" version="1">MVASPAEGLPRGSAEFVESSVLEAVVPAHSGIDIANELRTRDGKIEDESGSILPFLSQRNVLLLGMYSSDLASSACLLLMRRCLDELLSVYVVFRSPLLEDEILRSYLARLVVNVEAFAFSTAPPLENEPKTAPKEIIYSSIIKPEDEPTVVRHGEGDQAYTYVIWKVEVFISRPQGRFHKPAVYFQPTASFKPAERPRKVVSEDDYLPSRVPAPLNLLQSFDNDPALAGIHPRLSAMRISKIAPSAPIAREMVRPIRNGQRPLFRVLPPLVWRMSYSRLQTSLSDLSLMASLDLEVAKYTTYDVTIRELTLQLHGGQAKPLSNQEDSTVHKPGDHLTYLFKISPDLTSDGTPSLGNKGHFLILNLKAQVFISRDCQPDIAIEWKTPVDFTNEQTSSLIRAAQRLSNTVPSAKAPNPDSLPAHDTQSQQEQDALNNDINVTLTISGPPRVQVGEIFTWDVFIVNRSEKTRKLAVLVIAKRKRDYDSHKSHPSTSSTSDARTDKRSLLATAVIDENIVYAKQKGARTESAELICLTTDIRLGQLSPGACYTANLKFLALSTGALSVESVRVIDLATNETADIRDLPSIVAVDNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3Z2</accession>
    <name>A0A9W8Y3Z2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Geranylgeranyl pyrophosphate synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007872</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3Z2"/>
    <dbReference type="OrthoDB" id="5393654at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35179">
        <property type="entry name" value="PROTEIN CBG02620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35179:SF2">
        <property type="entry name" value="START DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="54358" checksum="B5163AEFEB543F77" modified="2023-11-08" version="1">MSFNASFSGNSRGGFAPRRGRGGWGRPLAKPREPVKPDIESHPLGQLLQTVHNSDLLDMPSNSSHNVTITDCQYVASYNWLNNSNPTVVVPGKPARWTPLQYPQKLKEDSGQYFRDLNAAKYPDFPMAPVAHAVLASDDFRPANVNLFACGSTLGNLLRFVRGMDKAFRFNVEAIGDTVFFIRKENDPKELIKDVRGFGHTFPEAYTTWEQEVKGSETHQRIVQYVFGNLKCMVRFECDGYIKDDPIGRLASSTLATSNNTSNSTSDEEDLLKSLQDTAISRPLKTTTSECEALTIKRGGSEIPQCSIFDLKTRSSRAKWGINMDDIYPLLWVKQIPNFIAAYHDGFGIFNDIRVQDVREDVQKWEKDNMKAIRRLSILLEKIIDIAKEDARGLLEVYFAGAGGLEIRSQHGEGMHALPSELMEKWADNEATRGVSDSDASDDTDQKFYDFESDDDWKAHSDEEEPDYTACSAEDCGYCGKCTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4P8</accession>
    <name>A0A9W8Y4P8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphomevalonate kinase</fullName>
            <ecNumber evidence="3">2.7.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Ergosterol biosynthesis protein 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ERG8</name>
        <name evidence="17" type="ORF">N0V83_007224</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(R)-5-phosphomevalonate + ATP = (R)-5-diphosphomevalonate + ADP</text>
            <dbReference type="Rhea" id="RHEA:16341"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57557"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:16342"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.2" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4P8"/>
    <dbReference type="OrthoDB" id="10262935at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004631">
        <property type="term" value="F:phosphomevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.890:FF:000018">
        <property type="entry name" value="Phosphomevalonate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016005">
        <property type="entry name" value="Erg8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035102">
        <property type="entry name" value="Phosphomevalonate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31814">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31814:SF2">
        <property type="entry name" value="PHOSPHOMEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017288">
        <property type="entry name" value="PMK_GHMP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="10" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="12" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="5 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="16">
        <location>
            <begin position="58"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4367639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="360" mass="39242" checksum="D26FDCD8D1A5FC1F" modified="2023-11-08" version="1">MQTLTYALTYISSLQPNQRIASSSIRILADQAYYSNPGIQRKADSRFLDFNVTLKDAHKTGLGSSAALVTSFTAAVLSFYLPKSLFDLQSEKGQIILHNLAQASHSHAQGKVGSGFDIASAVFGSCLYKRFSPSLLSTLPQPSSPGFATELQSLIEGDDWDTEIRKAAIKMPRGLRLVMCDVDCGSETPSMVKKVLAWRQENKEEADRIWKDLQQGNEALAAELTRLAVADSISGDGAEKHNKLREIIHQNRGLIRQMGESSGVPIEPPQQTHLLDYCSTLDGVVGGVVPGAGGYDAVVLLVEDNEDIIASLRKSLEQYKPEDSEEGLRIGKVGIIGVREDMVGVKTEDLSLYEEWPTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4Z7</accession>
    <name>A0A9W8Y4Z7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006907</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4Z7"/>
    <dbReference type="OrthoDB" id="3672266at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="618" mass="68720" checksum="D2E6B236BA03F5C7" modified="2023-11-08" version="1">MGPPTNTPEENTAFLDACIHKYRSLRDVYETLGLFCETAKPRKDPDFGKEEAKELHAEIREIARAFEGRHGNDLQHLKAHLANDDIFSDEIDDLERRYGQAIWGRAGPEKTLSFVEQGGVLEEFNWDDVKHREIVRFYVHCWIVSVVVGDVSSTPRKGKGKGKGKSKTINVPDREQVDIESPARSSRSDRSGPSELPYRAPEPPNMISASAIRGKNAQSKTLHLHKEGQRRNANTEDNASDNIDMGTGPPLKPILCSPTKRKAPGAQDPHSAGKLQKLSREAGTPSIPSGMLQGDVYSVPRSPTLPAGDAPLRSLAERQRCKGGGQNTDATCMPSPRGVTAETETIADDDEAAADAIDQVIHFPFLSEASIPFRYENLPTAGPSGTYRNPFRRDSVIPDAQNDKDGASPEPIESRTTLGVVVDSSAFNDTAAIPPRIISPSPQTWQGKQHRRELFTLLLSYLNGINQFGPDSYDTNAEERMDSLLIQLWALNEKQLRSELGNDFVRLDTALRGWMDMRKRLVAFQTSTGYLEQPGEPWRAYLRRMLDISGRAQLCIAFSELQAPWSDEGGLQYVDRSFDEDLETVFDLLTRVKGCNGAEEFKAIRVFNEALLGWFRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y584</accession>
    <name>A0A9W8Y584_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006851</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y584"/>
    <dbReference type="OrthoDB" id="3718497at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="219" mass="24354" checksum="376E51085B8DCF91" modified="2023-11-08" version="1">MAALRPHGSSLELTHYVSAAADSLPAVAVEIPVTRAPALVASQSKLETLPVEIVNQILSYFTHPRSRLPGLTEAQSAHDFPRQAKSDIKSKEDLTQPPDSDRWAADLFSWHLLSHPFHVLSLTSKRCNELVESYCSHLIRTKPGSVWVLRIDVESLALRLYGTRAFHNAHVQQMGKPCSICAITRFLPSQTRLTKSKSGQKTTRPLASLKKKVTKRSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5R6</accession>
    <name>A0A9W8Y5R6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5R6"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040942606" evidence="1">
        <location>
            <begin position="17"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="144" mass="13990" checksum="0C9F1D5C954758A8" modified="2023-11-08" version="1">MRFFTIAASLVAVVAAQYSAAEETCDVAVTVTVTETLKHTPSAPTGYAPTAPAPYPTTGYASAPAPVPSGSVPSGTGYPLVPEASTYSAPPPAGTGYPVVPSGTAAPSGTAASTSAYPEFTGAASGLKVGGVMMGAAGVVALFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5U8</accession>
    <name>A0A9W8Y5U8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nuclear architecture-related protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NAR1</name>
        <name evidence="8" type="ORF">N0V83_006876</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the cytosolic Fe/S protein assembly machinery. Required for maturation of extramitochondrial Fe/S proteins. May play a role in the transfer of pre-assembled Fe/S clusters to target apoproteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NARF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5U8"/>
    <dbReference type="OrthoDB" id="10253113at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.950.10">
        <property type="entry name" value="Fe-only Hydrogenase (Larger Subunit), Chain L, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050340">
        <property type="entry name" value="Cytosolic_Fe-S_CAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009016">
        <property type="entry name" value="Fe_hydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004108">
        <property type="entry name" value="Fe_hydrogenase_lsu_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11615">
        <property type="entry name" value="NITRATE, FORMATE, IRON DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02906">
        <property type="entry name" value="Fe_hyd_lg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53920">
        <property type="entry name" value="Fe-only hydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iron hydrogenase large subunit C-terminal" evidence="7">
        <location>
            <begin position="130"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4367295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="649" mass="69277" checksum="DDEE41C029ACBAD0" modified="2023-11-08" version="1">MSAILSADDLNDFISPGVACIKPIETLPVQSEDTSNPYEVTTEEKAAAAQPPPPASISLTDCLACSGCVTSAEAVLVSLQSHTEVLSTLDTHPSLRAPWAHDGTNGVAQAHGVNGVTNGVNGHHAEGKLFVASVSPQARASLAAVFGVSEVEAGNMITQLLSGPSGLRKGGHQGSDFTWVIDTNVVREACLVAAADEVANALSPESSNAPSKPGSEGAIDTTPKQPILTSACPGWICYAEKTHPYVLPHLSRLKSPQALTGTLVKSVLSERYNVSPSQIWHLAIMPCFDKKLEASRGELTSSAWLPNYDSSQGPVRDTDCVITARELLHLASARGINFASLPRSPLPSVDRTPFPDPKLDAFLFPPSRRKNQDPAAGPSGGYLYHILQTYQARNPGSTISISRGRNADVVEYSLVRGGETIFKAARFYGFRNIQNLVRRLKPARASRLPGGKTGVSRRPNGASAAVGEGVKEYAYVEVMACPGGCTNGGGQIKVSEVEDVRIYEGVQQPKDDDAPVVKPGPKEQKEWLARVDEAYFSGSDSESDQEQEQVKALDGDGDQTMQDVNTNTNGHTNGHIKHDGHDDDDIVNGISRNKINQVLTHWSVITGVDMQKLVYTSYIKVESDVGKTKKKQSDMERVAGLAVSVGGGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y659</accession>
    <name>A0A9W8Y659_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_007306</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y659"/>
    <dbReference type="OrthoDB" id="2520703at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4366781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="474" mass="53054" checksum="95430E39B6C61D75" modified="2023-11-08" version="1">MAAPATTLLDLPDELILEIIRPLSCIRSYETQSTAFKDKKKERARQHENHVRQLALHSICLASRRLRGIAIPTLYASFNASASLYGYERVRLFHRTITSLEHGLGLNSRLAEYIQYVEIRLADSHGNHLSNDLETLELSQYYYLLGEILMQVSNIQHLSIVSVENEEFSLWSPILPGHLAAGASTRLGTVASHGFPKLQTLNLQIHALGYDSSPIHAMFHRVCSAMTSVLSLTDLRTSSFSSTGSIKPLSGSFQSLRRLEITECELPFDEVLNLWSACEGLRHIVCSWAYLDGQGVTPSDLYTGLLRHSKTLETLCLNLREVRFDSPATQLRLGSLQPFTRLESLTICEIALLGTLISLEGPFGPDLHNHISDCLPESLKHLELLFTKGVGRDSGSDPSELDDLLELWHLAENCKSAMPELARVSVRSAFRRGAPRLTKAFNDIGLQFENFEETLQFENIEEALQFEVIEEALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6C2</accession>
    <name>A0A9W8Y6C2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005914</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6C2"/>
    <dbReference type="OrthoDB" id="10499907at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="192" mass="21449" checksum="EFD71125BA76DD22" modified="2023-11-08" version="1">MSQTRSGEDIMPPRPETPQPATERVKKLPAWHKWVEAGGLAEFAKAHGVEPKFVDRLCDVMPAIMIAYYERLRSVPGLEAEMAYNRWAGTPGFSWASSQSAMALAEGWDNSDEEAEEESEEEAEEEAEEEAEEEAEEEAEEEAEEEAEAEEEAEEEAEEEAEEEAEEEAEEEAEEEAEQEEAAVSLKSDSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6Y8</accession>
    <name>A0A9W8Y6Y8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Short chain dehydrogenase/reductase dmxR12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">DMXR12_2</name>
        <name evidence="3" type="ORF">N0V83_005527</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6Y8"/>
    <dbReference type="OrthoDB" id="5336600at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43669">
        <property type="entry name" value="5-KETO-D-GLUCONATE 5-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43669:SF4">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="150" mass="16015" checksum="A052E25261F17317" modified="2023-11-08" version="1">MTAEQQTVLILGAGPNIGAGVAEQFADNGYKVVLTSRKQHDDATPAYSYVQGDLSDPMSVVEIFAKVREISGEPSVVVYNAAAVSRTAKENTFDLELSTFESDLNVNTTSVFVAIKEALKSFTSLPASASKTFIYTYVSIQPPISKAGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y799</accession>
    <name>A0A9W8Y799_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HpcH/HpaI aldolase/citrate lyase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_007257</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HpcH/HpaI aldolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y799"/>
    <dbReference type="OrthoDB" id="1621678at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005000">
        <property type="entry name" value="Aldolase/citrate-lyase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050251">
        <property type="entry name" value="HpcH-HpaI_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30502">
        <property type="entry name" value="2-KETO-3-DEOXY-L-RHAMNONATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30502:SF0">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03328">
        <property type="entry name" value="HpcH_HpaI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HpcH/HpaI aldolase/citrate lyase" evidence="4">
        <location>
            <begin position="4"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4367672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="193" mass="20485" checksum="840A5C1B3EB9C12D" modified="2023-11-08" version="1">MDGALDAGAHGICVPLIYTVDDAKRLVSSTKFPPEGTRGFGSPFAVQCFNDEPLTYYLQNANSTLLTIVQIETASALQCVREIAALPGVDVLLIGPFDLGNNIGRPILTNEMHQDLKDAIEKIKDAAHAEGKKVGMYCATGEQARIYAERGFDMISISTDQIAITTLFSQALAVASGKADGSGDQGVKGYDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7E7</accession>
    <name>A0A9W8Y7E7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HD/PDEase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005604</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7E7"/>
    <dbReference type="OrthoDB" id="16547at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33594:SF1">
        <property type="entry name" value="HD_PDEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33594">
        <property type="entry name" value="SUPERFAMILY HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G03035)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="1">
        <location>
            <begin position="39"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="231" mass="25882" checksum="51F4399EEBFB6B35" modified="2023-11-08" version="1">MATNKQAPLPVPQVPIRDQDKELFAAINAFVHQYMSKYDNSHDYQHILRVVSNANGILQAELKADPSLKYDTATLFLAALLHDVGDHKYAKPGEDIENQIGTVLKANGASQDLALKVQTIVKHVSYTHEIRNPAAAISLIDQYPELAIVQDADRLDAIGAIGIARCFSFGAAKYPELPMSRAIDHFEEKLYRLEGMMKTGSGREMARRRTQVLKEFAKEWAEETELSFELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7L2</accession>
    <name>A0A9W8Y7L2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphotransferase</fullName>
            <ecNumber evidence="8">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">GLK1</name>
        <name evidence="13" type="ORF">N0V83_006269</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the hexokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7L2"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000034">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019807">
        <property type="entry name" value="Hexokinase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF30">
        <property type="entry name" value="GLUCOKINASE-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00378">
        <property type="entry name" value="HEXOKINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase N-terminal" evidence="11">
        <location>
            <begin position="127"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="12">
        <location>
            <begin position="346"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="548"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="548"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4369184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="643" mass="69890" checksum="2B13FEBFD122892F" modified="2023-11-08" version="1">MVNESTARNSSSSASPRSPIKATSPPNSTPRRREPAGSPLATHATVSLVVVLLALVLQFFAPHFPHTILQFFTQDKHWRVSSPGITLRNPFLFQDEPAICPKPEQHVEFVGTFNTKPERSMALAEQAKRVAAEFEFGPDAVNKAVKEFIREMDEGLQKEGTELSQIPTYVTAVPNGTEKGLYMAVDLGGTNFRVCSVQLHGNTSFSLTQSKVAIPRELMVAKTSKELFSFLAKQIENFLKAHHEDHYAGTLRRRETGGSLEQEEIFNLGFTFSFPVQQIGINKGMLMRWTKGFDIQDAVGKDVCALLQAEIDELHLPVKVAALVNDTVGTLMARSYTSPGKSGTLLGGIFGTGTNGAYVEKLNKVTKLTKSKDAGDYDKSTGEMIVNTEWGSFDNSLRTLPNTPYDIELDEKSVNPGIQMFEKRVSGMFLGELLRLALVKLIKDPAIPLFKDENSSSNDVHSTTQIHKGSPIWEQWGIDTSFLSVCAGDSSPGFRMLRQTLDKDYDISAVSAEDAEAVREIAGAIGRRAARLAAVAIAGIVINTGRLSSSSTGTATTESKTGINPPRGDVEVSEDDVIDVGVDGSLVEFYPNFEEYIREALKEVPEIGQHGEKRIRIGIAKDGSGVGAALIALVAGKVAAPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y869</accession>
    <name>A0A9W8Y869_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y869"/>
    <dbReference type="OrthoDB" id="4172108at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="353"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="757" mass="84461" checksum="AF25C370312A532B" modified="2023-11-08" version="1">MSFGFSPGDVIKLLEISTRVYIAFKDANENSEAQVEGLVREFTAFHQCLVELGELMKEYGKPLPFPCKDFEVTLEKCEKSLEPYAENLVDKKMGIKKIIYTVKYMGKEKEIEGLRNQITGHYQALHMCISFLQLRLHLEATKQTQRLLDAAPFRSMSLGGRSYTTNALGNSSQGALLALPAPAEHPLYSEWRIFDRWLQSEDERVAQEAGLARPLSLGDTPAAAPTGDVETAAILYQLRRQVDDAILFEENRAKRTTAEKRSLLAPSDSMKQKVRGMPPAPQRTYTLQTDHSGDFFGFDLHNMSISTTTIRPGPSTPSPSPSPSGSPRIESSYFGSIDWAQSPTEGTMSQDVGNRSSISTTRSSFSNSPGSRLTVNGLGISTAGTTPHSAMSRPLHHKLSTTSLATIALGEGALDWNSLCRKVQVERKSVEHVHGRERTIVESKECDIHWRYREDTGISLRALYRSSKDNKPKPWTVQHFPATGPSIPLTTTYPDGEVSVDFPRGSFGRLDKHWTDIKYTFTGYDSAEKFQTLLYTNNGTDAAELKYDRPILSISSDKNSTECRGRNLRIWRRTETHLDHDGPVTFDVLVLLFYTSALEDKGHWVEEPHYAFEWLADSTYKRDSEKLTLVFSKDPAKWTTDKLFQRRKSSKGSFSNDGTATAGGAPRSPIFLRKLNDSMEIPELTRSGTRASVALSAMSIKSSKSLFGRSSGGSGTGNLNRFGYSELQIKFQSGKDRRAFLDVWKEYVKPLGAKASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y910</accession>
    <name>A0A9W8Y910_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL30m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MRPL33</name>
        <name evidence="9" type="ORF">N0V83_005927</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y910"/>
    <dbReference type="OrthoDB" id="509901at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01658">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000010">
        <property type="entry name" value="Large subunit ribosomal protein L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005996">
        <property type="entry name" value="Ribosomal_uL30_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01308">
        <property type="entry name" value="rpmD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15892:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15892">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="8">
        <location>
            <begin position="4"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4368845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="92" mass="10581" checksum="F86B7BC1590D2654" modified="2023-11-08" version="1">MSYFRITLMRSGIGMPLKTQGVLKALGLRKRMTTVYHPVTQSVAGQIMRIKELVDVKEVKNMMTNEQMRAARRPDPGYYIEQRAGEAMRGYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y927</accession>
    <name>A0A9W8Y927_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y927"/>
    <dbReference type="OrthoDB" id="4502478at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="108" mass="12745" checksum="5CA2CBD67AEF80AA" modified="2023-11-08" version="1">MILTDMSRADPAVTTPYKWDEISETAKHLEILTVVNNARPETRPYYHMGRYMTNVNEENWVARWYLWHSFRYRDNRPRSTTNNGSEGYGGGQGPYGAGYYDPARERYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9B9</accession>
    <name>A0A9W8Y9B9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NADH-ubiquinone oxidoreductase 78 kDa subunit, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NdufS1</name>
        <name evidence="15" type="ORF">N0V83_005680</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the complex I 75 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9B9"/>
    <dbReference type="OrthoDB" id="10249365at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02773">
        <property type="entry name" value="MopB_Res-Cmplx1_Nad11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.740:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.210:FF:000002">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 75 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000002">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 75 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.740:FF:000033">
        <property type="entry name" value="NUAM protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000283">
        <property type="entry name" value="NADH_UbQ_OxRdtase_75kDa_su_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054351">
        <property type="entry name" value="NADH_UbQ_OxRdtase_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010228">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019574">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015405">
        <property type="entry name" value="NDUFS1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050123">
        <property type="entry name" value="Prok_molybdopt-oxidoreductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01973">
        <property type="entry name" value="NuoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105:SF13">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 75 KDA SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105">
        <property type="entry name" value="RESPIRATORY NITRATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13510">
        <property type="entry name" value="Fer2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22151">
        <property type="entry name" value="Fer4_NDSU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22117">
        <property type="entry name" value="Fer4_Nqo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10588">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09326">
        <property type="entry name" value="NADH_dhqG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00929">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51839">
        <property type="entry name" value="4FE4S_HC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00641">
        <property type="entry name" value="COMPLEX1_75K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00642">
        <property type="entry name" value="COMPLEX1_75K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00643">
        <property type="entry name" value="COMPLEX1_75K_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="12">
        <location>
            <begin position="87"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S His(Cys)3-ligated-type" evidence="14">
        <location>
            <begin position="165"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="13">
        <location>
            <begin position="304"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4369916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="802" mass="87622" checksum="A2309609552823F8" modified="2023-11-08" version="1">MAGSERILEGAAGALANHAADYEITPRQGKAHPIFQVKVELPSSKSIAIVQSPKMLRQQLIRSLRRPAHRAALNGSRAFTASAPRPAEVELTIDGKKVSIEAGSALIQACEKAGSTVPRYCYHEKLMIAGNCRMCLVEVERAPKPVASCAWPVQPGMVVKTNSPLVHKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMRYGADRGRFHEIGGKRAVEDKNIGPLIKTSMNRCIHCTRCVRFSNDVAGAPEMGSTGRGNDLQIGTYLETALDTELSGNVIDLCPVGALVSKPYAFKARPWELYKTETIDVLDGLGSNIRVDSRGVQVMRVIPRLNDDINEEWINDKSRFACDALSTQRLVNPLIRVDDQFQPATWEQALVEVGNAYKKIAPKGNEFQAVAGHLVETEALVAMKDLANKLGSDNLALDQPGGSDPIAHGIDVRSNYAFNSRIYGVEEADAILLVGTNPRHEAAVLNARIRKQWLRSDLQVGLIGEDFESTFEYQKLGDSATDLKSAFSGEFGKKLQSAKKPMIIVGSAVTEHADAKSIYEQVGAFVEKNKATFQTQEWNGYNILQRTASRTGAFEVGFTVPSPEVAQTKPKFIWLIGADEINEADIPKDAFVVYQGHHGDRGAQLADVVLPGAAYTEKSVTYVNTEGRVQMTRAAVGLPGASREDWKIIRAASEYFGAELPYDDVEALRDRMEEISPALRRYDLVESVSLPALSKVQLVDQNKGSQASGKPLKKPIENFYFTDSISRNSPTMARCSAAKAEKNPKTNFMAPGYPNPQVHYGPDPTAAVANV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBQ6</accession>
    <name>A0A9W8YBQ6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Bifunctional cytochrome P450/NADPH--P450 reductase</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="17">Cytochrome P450</fullName>
                <ecNumber evidence="17">1.14.14.1</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="17">NADPH--cytochrome P450 reductase</fullName>
                <ecNumber evidence="17">1.6.2.4</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="21" type="ORF">N0V83_003157</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>2 oxidized [cytochrome P450] + NADPH = 2 reduced [cytochrome P450] + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24040"/>
            <dbReference type="Rhea" id="RHEA-COMP:14627"/>
            <dbReference type="Rhea" id="RHEA-COMP:14628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:55376"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="1.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>an organic molecule + reduced [NADPH--hemoprotein reductase] + O2 = an alcohol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17149"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:142491"/>
            <dbReference type="EC" id="1.14.14.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <cofactor evidence="17">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17 18">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">In the N-terminal section; belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.1" evidence="17"/>
    <dbReference type="EC" id="1.6.2.4" evidence="17"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBQ6"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070330">
        <property type="term" value="F:aromatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003958">
        <property type="term" value="F:NADPH-hemoprotein reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06206">
        <property type="entry name" value="bifunctional_CYPOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11068">
        <property type="entry name" value="CYP120A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000040">
        <property type="entry name" value="Bifunctional cytochrome P450/NADPH--P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.990.10:FF:000011">
        <property type="entry name" value="Bifunctional cytochrome P450/NADPH--P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000198">
        <property type="entry name" value="Bifunctional cytochrome P450/NADPH--P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000032">
        <property type="entry name" value="Bifunctional cytochrome P450/NADPH--P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000031">
        <property type="entry name" value="Bifunctional cytochrome P450/NADPH--P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023206">
        <property type="entry name" value="Bifunctional_P450_P450_red"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF127">
        <property type="entry name" value="BIFUNCTIONAL CYTOCHROME P450_NADPH--P450 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000209">
        <property type="entry name" value="Bifunctional_P450_P450R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 17" id="KW-0274">FAD</keyword>
    <keyword evidence="5 17" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 17" id="KW-0288">FMN</keyword>
    <keyword evidence="4 17 18" id="KW-0349">Heme</keyword>
    <keyword evidence="12 17 18" id="KW-0408">Iron</keyword>
    <keyword evidence="7 17 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 17" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9 17" id="KW-0521">NADP</keyword>
    <keyword evidence="11 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 17" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="19">
        <location>
            <begin position="499"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="20">
        <location>
            <begin position="677"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="18">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000209-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ4372866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1068" mass="118681" checksum="E13B7EC9C476D3C3" modified="2023-11-08" version="1">MEHETIPGPPGLPIVGNLADIDATNPIQSMCNLTSQYGPIWKFHLGGAERVVVGSQALMDEVCDEERFSKIIAAALDQVRNGVHDGLFTAKGPQEKNWGIAHRVLLPQLGPLAIRNMFNEMHDIASQLVMKWARHGSDHTIQVTDDFTRLTLDTIALCAMDYRFNSYYSEHMHPFIDAMADFLKVSGDRARRDMITQMFYRAETQKYWDDIELLRKTSQQVIKTRRANPTDKKDLLNGMLNGVDPKTGEKMSDESVIDNMITFLIAGHETTSGLLSFTFYYLLKNASAYDTAQQEVDDVIGKGPITVDHLTKLPYLNAVLRESLRLSPTAPSVGLTAKEDTTIGGKYPVKAGAPIVALFPMIHRDPIAYGEDAEEFRPERMLDEEFDRRNKEFPNCWKPFGNGMRACIGRPFAWQEALLVLAMLLQNFNFSMEDSSYQLQIKQTLTIKPKDFYMRAHLRNHVSATSLERALASATIADPSPSNSAPGVKKEKATKGKPMAIYYGSNTGTCEALANRLASDAANHGFRADLVDTLDAARENLPTDKPIVIITASYEGQPADNAAHFVNWVGTLKEKELQKVSYSVFGCGHRDWAKTFLKIPKYLNTTLEERGATRLVDMGSTDAAQGDIFTDFETWEDQTFWPAMREKYGSTEADGEGSLEATLDVEISTPRSSTLRQDVREARVEEVKVLSSSGVAEKRHIEISLPSDMTYSAGDYLAILPLNPKENIHRAMRYFGLSWDSMLTMSSAGPTTLPTDTPISAIDVLGAYVELAQPASKRNVLALQEATPDESTKKELSRLADEAFTEEITAKRVSVLDLLERFPSVQLPLGVFLKMQPPMRVRQYSISSSPLWNPTNVTLTYAVVDQPALSGQGRYVGVATNYLSSLTPGDKLHVSVRTSHQAFHLPKDSKVPVIMIAAGTGLAPFRGFIQERAAQLAAGRSLAPAILFYGCHAPDSDLLYADLLKRWEQLGAVSLRYAFSRAAEQSEGCKYVQDRLYHDRADIVEQFDAGAKLFVCGSRGVGEGVSETLIKIAKERAREVKGREVDDAKAREWFEGLRNERFATDVFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCA1</accession>
    <name>A0A9W8YCA1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sorting nexin-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SNX3</name>
        <name evidence="14" type="ORF">N0V83_002550</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Required for retention of late Golgi membrane proteins. Component of the retrieval machinery that functions by direct interaction with the cytosolic tails of certain TGN membrane proteins during the sorting/budding process at the prevacuolar compartment. Binds phosphatidylinositol 3-phosphate (PtdIns(P3)).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Prevacuolar compartment membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sorting nexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCA1"/>
    <dbReference type="OrthoDB" id="5227681at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030904">
        <property type="term" value="C:retromer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034499">
        <property type="term" value="P:late endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07295">
        <property type="entry name" value="PX_Grd19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000030">
        <property type="entry name" value="Sorting nexin-3, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042138">
        <property type="entry name" value="PX_Grd19_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051074">
        <property type="entry name" value="Sorting_Nexin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45963">
        <property type="entry name" value="RE52028P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45963:SF2">
        <property type="entry name" value="RE52028P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="13">
        <location>
            <begin position="21"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4373811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="142" mass="16500" checksum="028A2F9DF783F3CC" modified="2023-11-08" version="1">MQSLPDARSQSFEELYGPPENFLEIEVRNPMTHGVGRGMYTTYEIVMRTNIPAFKLKSSSVRRRYNEFEAFRDILERESARVTIPPLPGKVLTNRFSDDVIEHRREGLQRFLQIVVGHPLLQTGSKVLAAFVQDPNWDRHAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCF4</accession>
    <name>A0A9W8YCF4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">mRNA-capping enzyme subunit beta</fullName>
            <ecNumber evidence="8">3.6.1.74</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">mRNA 5'-phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">mRNA 5'-triphosphate monophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_002625</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end diphospho-ribonucleoside in mRNA + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67004"/>
            <dbReference type="Rhea" id="RHEA-COMP:17164"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167618"/>
            <dbReference type="EC" id="3.6.1.74"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:67005"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer. The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an mRNA 5'-triphosphate monophosphatase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the fungal TPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.74" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCF4"/>
    <dbReference type="OrthoDB" id="272147at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031533">
        <property type="term" value="C:mRNA capping enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140818">
        <property type="term" value="F:mRNA 5'-triphosphate monophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004651">
        <property type="term" value="F:polynucleotide 5'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07470">
        <property type="entry name" value="CYTH-like_mRNA_RTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.10">
        <property type="entry name" value="mRNA triphosphatase Cet1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040343">
        <property type="entry name" value="Cet1/Ctl1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004206">
        <property type="entry name" value="mRNA_triPase_Cet1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037009">
        <property type="entry name" value="mRNA_triPase_Cet1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28118:SF1">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-TRIPHOSPHATASE CTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28118">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-TRIPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02940">
        <property type="entry name" value="mRNA_triPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="CYTH-like phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="4 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="mRNA triphosphatase Cet1-like" evidence="10">
        <location>
            <begin position="148"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4373886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="433" mass="49021" checksum="162EB8314E2B8607" modified="2023-11-08" version="1">MDISALVNHDTEAAVLPRRSLSNPHSLLSTPVTSAARIPTPPKTTPRQMPLKRKRNDPKPIWAYREGEELPPELKLQQEQRQQSRPPPPATQPQPQPQPQPQPQASPMVQRNGPPPSAIDAPSQPATATTTELSGFERPVSDDAQVYDDVSRQVCDFIWVTAVNNDLVRNAIQDPQTQLEVEARWGHIIDKRSDQRFRGYHSSECVIKTDNMEVKFESTMTIEQHKRMNMYLNGQVQKAKAPGALRASVDYQHTKEIDLFYELDQQGFLSLNPILQRLINQSGNRQRIRVTKDAKTGQVLRAIIKHRIANLEISSPQTEWDYRIGINLEINFAGPVENLKLAMEPGKTPESMQRHKDRISYSWLGAYQVDLTQVTQGQSRNHELELELDAGVLISEADKVRMKEDNKFEPLINGMMNNLRVLSREITPPAPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDB5</accession>
    <name>A0A9W8YDB5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fungal N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001894</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDB5"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="19582" checksum="3EE336D1167885D6" modified="2023-11-08" version="1">MADPVSGIITIVTVSLAIIQQTVKFIQEANVIDESTKRLLVKLKDLHKQIREVESLCKHAGSQEDEPSRFVCEDLKRCNDRLDQVRTTVEALALRSSGTFMQKATLKIRSERSKKEIEDAITDIKDLIVLIHNSIICWTYRTVTIIARRPSEAPPSYETNQTKQYEIPVNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDK9</accession>
    <name>A0A9W8YDK9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001867</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDK9"/>
    <dbReference type="OrthoDB" id="2851338at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43039">
        <property type="entry name" value="ESTERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="316"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="566" mass="63628" checksum="AC747157DF1E9059" modified="2023-11-08" version="1">MASKSTPGILYVTMQPKEGLPDAQFHDWYQNEHGPNRLRLPVCNNGFRYRAKDLENQPGSKDKPEWMAIYDFDELEWLTREPYTRLRSAPVQSQRERDTMKQIFVDRRSYDLLKEWKADDFKDFQKVENEGEQNVMIAVSFVLQDGEGKDEELRKWYEEEHVGLLQKVPGWRRTRRFETSYLDLESGHKTEKEYLALHEYAPQNGLGGPEFKTATTTEWCDKIYKDVVKGRNRRVYDLYYTFGAASRDLSSLSSTDAISAEFVEGLTKTHPAHKAPEKWPAIESFVTTPDGVQLPYRLEGSASPNAPVLVLANSILVNHGIWDDFVSHFLRLTNNKYRILRYNTRGRNALPPKSITPVTLDTLTTDVITLLDALRIKSAAIAGVSLGGATALNVGLEYPNRIEAFVSCDTNAFAPPSNAGAWNERVAVAEKESSTSSTSSEPIVGEQLAELTVRRWFPQSSYDDATLAKRIARVKDMVATNSLPGFRDGVKALHQYDIREKMSGYQGKGVFLVGSEDGVLPKSMKENMAAKLGSGVELKVVQGAGHLPMVERPEEVAGFVAKFLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDM1</accession>
    <name>A0A9W8YDM1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_002061</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDM1"/>
    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Homeobox" evidence="7">
        <location>
            <begin position="80"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="288"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="82"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="149"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="188"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="232"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="260"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4374982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="706" mass="79142" checksum="C14A01C919876273" modified="2023-11-08" version="1">MYSNEFGASFLFPSRDDSLGLNHVLDDGGFAELSEALAAAQAGPSNSDNIWTLEPALQDLSNSSTALSSPFSSSTPISSITRPKLGSRFSREVIRTLKNWLAVHKNHPYPKDDEMSELQEQTGLNKAQLTNWFANARRRGKVQTIRPASPQVRNVSTGPIDIIPRPATPAVGQASYSKNPMQRWVESPPEHEPAAVRDIARAMASTSRKSSVENNAHDSEYAYNDPWRSPYARSSADSGGTSRSSEFSGLHSSGSQSSLKVRRAPRRKRAARRKRIEKDPTADPSMPYQCTFCTEVFKTKYDWQRHEKSLHLPLEQWVKGDWGFSDVVLNMVENACPPYFIDYERTTLIPMKGSDPPWGSPPNAYELLKVEIEFFVQNYFDTNSQLPSNDAMQLEACRIIFAAESVQTTHLSDVEAVQSVSWLCDLIMSSPELTRQARFGPIRTSSESRHTQITINGKDHIFEQCPLEAQLQAFITGQQLVDEVPDDAQLTKEACDIVRRMEAASHRPSDIFANWLVKGIWSGTDWLSSFKKRAGISEANNGVNVAEMPPTMQGWDTQWPPDSLFPLAASNTVATTDPHLKQDYTQDISSLTALEELTLTSPMTSSAHAHRSTFLPDDTNFFRCFEKDVKRWVAATMSPKNPNCHVPSDEEIQHQARWIMYDGDDSWNQTPADYAEWLWRLKRDVGILKDVNAIDPAELNIGGVHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDU0</accession>
    <name>A0A9W8YDU0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDU0"/>
    <dbReference type="OrthoDB" id="3799675at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="350"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="402"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="142"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="222"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="350"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="402"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="437" mass="49697" checksum="6BA2F75884835D31" modified="2023-11-08" version="1">MEGITAIVPGALAVSEAVATPNAVASGAEASNGDKRVAIEQGGKIVVKEPYYPEITGPQDRAPDRPLRPALQYSDLTRHIEWAEWAEQYVKQLEEKIVEGEIGRAEDKNKAAAKMAELRDEYKKRLRTARSGSVATANAQHEKEVRAALRKRENNIKVAEAKARRMQEESKTYQDLCDGIQSLGNRTETALRQMRTLTAAKNRAQAAFNRWETSFCEPNIRFLDLNRKIKEEREEIRQHHAEQMELVCDQKEQLDKLTQQKVEELASLDEQLRDLAAEKKAWRLQKGQELSELSESEQKGAMEVWMEQKIEEIKPLIMQEATKHAWSLQKPQWELQTAAALEIAEERGHKRGHWEGDKEGYVRGHKEGDEEGYARGQLDGYEKGTTDANTFVSRLIKQARSKAREGAYKEGHKTGLREGGYSSFAEGFDMGREYEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE55</accession>
    <name>A0A9W8YE55_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoglycerate mutase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE55"/>
    <dbReference type="OrthoDB" id="10261749at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052765">
        <property type="entry name" value="PGM-Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46192">
        <property type="entry name" value="BROAD-RANGE ACID PHOSPHATASE DET1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="361"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="423"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="441"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="485"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="549" mass="61784" checksum="D1CCE2010D5F60C1" modified="2023-11-08" version="1">MGKPRMIILIRHAQSEGNKNRDIHQFIPDHRVKLTQHGWTQAEEAGQTLRSMLKPDDTLQFYTSPYRRTRETTEGILRTLTSDDPTPSPFPRNKITVFEEPRLREQDFGNFQPCSAEMERMWQERADYGHFFYRIPDGESAADAYDRVSGFNESLWRSFSDDNFPSVCVLVTHGLMSRVFLMKWYHWSVEYFEDLRNVNHCEFIVMKRSENNGRYILHNELRTWSELKRRAAKEGATGASSTTSNRPTPLSNTGQVGLNSSPTIPIRRWGGCVNGCNHDKINYPRRPMRKNTMEYMGGQSQQLPPPATSSSGPLDGEQEDHPVAAQDADHTPAINDPTPGKLSRKQSTKTSSALAIPLTPASPNDASNDASSSEGDDATSATRSSNQHTAPRTAAIMRHLQSPQNSAGEGFSDDSDYFPGMQHLHHSPNGHRKVLRASSRQRQESKERKLARRQTEQSWKEESGMGTGARTDRLGDGDATSDEPGSAKERGTAEDQPILREALKGNMIVERSEEERERSDEDLDGDKTIGAAEVEKMKEAERKGFGEVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEE8</accession>
    <name>A0A9W8YEE8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002443</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEE8"/>
    <dbReference type="OrthoDB" id="1057137at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047574">
        <property type="entry name" value="AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039683">
        <property type="entry name" value="Lsm12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019181">
        <property type="entry name" value="LSM12_ABD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13542">
        <property type="entry name" value="LSM12 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09793">
        <property type="entry name" value="AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00995">
        <property type="entry name" value="AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52001">
        <property type="entry name" value="AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AD" evidence="1">
        <location>
            <begin position="125"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS52001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="243" mass="25752" checksum="A1ECF3932A206C17" modified="2023-11-08" version="1">MADNKRNSIAGKIATPNLKAGSGSNKDVIDADSNLYKAVGSRIKITCAPNNNVLEGTLFTACNLTNAIAINTAPAPPNPSAPLSSQPGDYHIIPFAHILSFEIIGSGERASESTPGFDGALPSISKVDLAALQAREEQTIREMKKKDAQKGKGVSKEAQELFDAISRTLPTRWADTQIVVSDSVLIQAPYTVDNIRAPADKTQAENHVRKIVEHYYQRKKGATSAGAAARAPVAVPVAPRKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEJ3</accession>
    <name>A0A9W8YEJ3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002894</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEJ3"/>
    <dbReference type="OrthoDB" id="5596991at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43111">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43111:SF1">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="466"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="2">
        <location>
            <begin position="29"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="499" mass="54551" checksum="51DA3F9B6A136726" modified="2023-11-08" version="1">MSSPLTPDVALANLQATALTGRCHNAFFRQKQLKFLHDTLRNNSDAITNAIKQDTHVLEEEAVTEVALSLTVVKEHYDSIDVKKELENEYRVANGKDASDRREPRGVVYIEPQRNHTPFFSAIAPLSAALAAGNCVALKIENNLRAVPSILRKLLSESLESDTFAVISSNPSPASLSPCLQVLQDTQINLPNYSQLVSPRSRTIAVVDRNADLALAAEQLVMARFAFGGASPYAPDLVFVNEFIKKEFLGHVLKHSIRLLTISNGIANGSSTAKESKKASRVREAIQALSDSKTWKLQIITQGDNGAIVELPNLSALPPKTKQPVFSISPITSLEHAISLIDEDLDIQDTLLAAYHFGTPSTGKYLSQFVNADASFVNHIPFRLLVGPSAPSFHPVDIEKRYTVKHFTRVSPAYVLPPVSQAAMSKIVASKDSRKAAAELLAKATEEIQEKKRAEWIAIGYFEQGIFIGLGLYGIPLVGCIGASLFFGLRAGLRRWAFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEN8</accession>
    <name>A0A9W8YEN8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_002954</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEN8"/>
    <dbReference type="OrthoDB" id="3781946at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4374213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="497" mass="58603" checksum="2AFA62DA256FF05E" modified="2023-11-08" version="1">MPTTLVQASQFDYQDPFFNDSDKIWSTEHIEEHPFVQKIREGVLQYRLQSASHFEVQIDVHQTRSSAYAGRSTKLKPSFKIEILFHDFNARRLLRICRLKPQQGSCTIREDVVGNIQVRDDDRYISLYEFLDTHFPTWPQAVKAETLHAWWNENGKTFDWTGLPTELKERIIKFCMHRSLVTRHTYDIRYYPRKREPRRSGPYEVADQLGRWSSLLGVSRQVRAITLRLCFVGHSRIYCQGLSIRAYSRLGLKDIVKRLGACYQLLEPNSLPLDDNARALAATHQKYPKIFPELRQYATLWHGVRRVSLQMDFLEYFHFFKVRVGGFEKFWRLNYVDYEVFEQLPHLNEINIKLPRLSGRLEDKSTQPGPPLFYEDFVCPRVLDQFIYEQAAKILAPYRKVKMYGFIDNTEALRFKALLEDAIQTLKFTSQELRELYMEDGGGIVLEEIVIPGVHISETEDRQTSIQRPVEYQREFWPPKCRCAVRCRKVILGDQDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YER2</accession>
    <name>A0A9W8YER2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_002447</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YER2"/>
    <dbReference type="OrthoDB" id="47059at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050288">
        <property type="entry name" value="Cellulose_deg_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011658">
        <property type="entry name" value="PA14_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715">
        <property type="entry name" value="BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715:SF3">
        <property type="entry name" value="BETA-GLUCOSIDASE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07691">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56988">
        <property type="entry name" value="Anthrax protective antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PA14" evidence="7">
        <location>
            <begin position="23"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4375361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="482" mass="52929" checksum="54E68EFCDA029694" modified="2023-11-08" version="1">MRRLGAHIFRVYPDLETGCTNARGDPGFLAEYYMTPTVTNEPFHTEEYPRGSFTTLMNTKVTGCQTVRFTTTYRPDVSGSHYLSFSGLGPSKLFINDQLIAEQTEPTKDAMGFLLGVQDELHMQYPFEAGKPYTMTIITQPSPTSNAELYLLDDQISTHLGLIHQSEMESDILAEATALAREADLAIVFVGNTTQWETEGQDLSTMTLPAPTTSTDTSSSSQNTLIAHVAAANPHATIVVNTTGVPIECPWLPAVPAFLQAWYAGQETGNAILDVLLGDVCPSGKLPVSWPRRYEDTGCFGHFGLDSFESRRVEYVEGVFVGYRWFDEWWGGEREVRFPFGFGLSYTAFEISDAKVEGAVERDDEGATVKVSAKVKNTGPVPGAETVQVYLAPPPPVVPSSGTPPRPQKSLVGFAKVELRPGEEKEVQVQFGRSEAAYWDEGLAAWRVTGGKYEVLVATSSHPRDCKVRLGMCVQEEFTFAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YES3</accession>
    <name>A0A9W8YES3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">N0V83_001176</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YES3"/>
    <dbReference type="OrthoDB" id="28092at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058545">
        <property type="entry name" value="Beta-prop_EMC1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026895">
        <property type="entry name" value="EMC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011678">
        <property type="entry name" value="EMC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573:SF0">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25293">
        <property type="entry name" value="Beta-prop_EMC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07774">
        <property type="entry name" value="EMC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040745310" description="ER membrane protein complex subunit 1" evidence="13">
        <location>
            <begin position="19"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="905"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="domain" description="EMC1 first beta-propeller" evidence="15">
        <location>
            <begin position="18"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="ER membrane protein complex subunit 1 C-terminal" evidence="14">
        <location>
            <begin position="709"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="743"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="752"/>
            <end position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF25293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4375898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="936" mass="102054" checksum="CF366A80D2FA230D" modified="2023-11-08" version="1">MRFHAALSLAACLAPAAAVFEDDAYHIDFHYALLGLPKHEATFFQKPYAGSKASLLYSISQNQTIGAINPKDGALVWRQHFESEHRGKGHLRSGNEQDTVVSAVGDRITAWSASDGRLVWETTVAGAVVEDLEILEQEDGTTFNEAKDVVVLLGDGNHGVQRLDGKTGRVKWTFEDTSGDTPFQVSTSPTTIYYISLHSPMLGGSKLRVTSLSPVTGKKLDQYTLNSDTEINSREHIFFAGANTAAPLLVWTDKANKVLKVNIIGAKSVASFNTPSDAAVEKIVLHAPNHVNSLPHFLVEYQTAAGHSAEVFHVDLKKNTLSKAYSLPELRSKGTFTTSTSDANVYFTRIIGDEVTVFSSVSHGKLGRWALKGTPVLAGAYPVHGVSEVVVKSGTASAVRSAVLYSNGQWALIRNEEIAWTRPEFLSHTVSAVFAGLPEEEALAQALADESHRNLVSAYVHRVQRHIQDLKFFPAWVQGIPSRLLSSVFGSSKETTVDDIQQDTFGFHKLVVVATEKGRLAALDVGSRGKVMWNIDLTQFAPETNFQSPSLKAFSGYVEVKVPSFKGSLFVNSTTGGLLPSSDIKAQAPLTSEGKAIVSFTLEEDTLKSFLERQPTSEPVWSFKPAVGERIVSYSVRPIRDPVASIGRVLGDRRVLYKYLNPNLVLVIAVVDSARTASIYLLDSASGQLLHTMSHDDVDTSRPIPSTISENWFTYSLTIDASSQASSRGYQLVVSDLYESPLPDDRGPLGANDNSSTVQPSGSKGEAIKPYVLSQSYQVPGEISHMTVTQTRQGITSRELLITVPSFSSIIGIPRSFIDPRRPIGRDPTAHEQAEGLVKYTPLINFDPKWHLTHKYEVLGVQDVITSESGIESTSLVFAYGSDIFGTRVAPSFAFDILGKGFNKISMLLTVAALFIGVLFVAPLVRRKQINTLWTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF75</accession>
    <name>A0A9W8YF75_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SURF1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SHY1</name>
        <name evidence="6" type="ORF">N0V83_002581</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Probably involved in the biogenesis of the COX complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Mitochondrion inner membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SURF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF75"/>
    <dbReference type="OrthoDB" id="10040024at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06662">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002994">
        <property type="entry name" value="Surf1/Shy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045214">
        <property type="entry name" value="Surf1/Surf4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23427">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23427:SF2">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02104">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50895">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4373842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="334" mass="38527" checksum="3336E777F7E72654" modified="2023-11-08" version="1">MSKPNPFLNLFRRYYSQNASTFARSKSALRPSRTARPQCTRTRFAPQFFVFRRNVGGGPGLDPNFVSILDRPAKMARVGKQHGPGLIILAIIPITAFILGCWQVQRLGWKTELIARFEDRLTFPPLELPLRIDPQAIKDFDYRRVYARGVLRHDQEMLIGPRILDGEEGYTVVTPLERKDEKGNIHKILCCRGWIKKEASPQWFRKKNGALPEEEVTVEGLLRIPPKGNMFTPKNEPEKGKWFFPSVEEMAQHSGSQPVWVEETMTPDLLTNYEREPKGVPIGRAPTVNLRNNHTQYIFTWYALSFATSVMFWMVVKKPMSGTQRRVRHSVDWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFD2</accession>
    <name>A0A9W8YFD2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Gluconate transport-inducing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">PTH2</name>
        <name evidence="2" type="ORF">N0V83_002457</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFD2"/>
    <dbReference type="OrthoDB" id="5572844at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018608">
        <property type="entry name" value="Gti1/Pac2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28027:SF1">
        <property type="entry name" value="CAMP INDEPENDENT REGULATORY PROTEIN (AFU_ORTHOLOGUE AFUA_3G09640)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28027">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09729">
        <property type="entry name" value="Gti1_Pac2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="311"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="442" mass="47264" checksum="BD3F614C7EAADEBC" modified="2023-11-08" version="1">METYHGHVRTPNDAIILFEACRIGILPRVQRRLSEKERQQIKSGSVFVWDEREAGMRRWTDGKSWSASRVSGSFLTYREMEGKRGGSALPTPPTAKRASGKSPEGSATGDSDGEGPDGYRYKPDGLMKQSFSITTSSGQHLHLISYYSRSNSNNLPQPTNDAQLRHIRPPKNMYPESTVNEAQPVPAVTRGPMPGSPYSAAGHSMAPTSPYARAGPVAPMYAVPVYPPTPPNGTPPMYAYYAQHPYFNGSVIYAQPPYPPPGHVFDRPPPPIANPAGPPGHPMLVHHPVYAAHAAHAAQQYYPQAPPPRTASAAEQAQHQQPPPAPAASIPEQGPQLPAITGAAASTGTNKPPTPEPSDKSQPQAPETNGAAAASRPLPTLGSLLNGDGPEKENQSNSRSGSRSPNTASRFPRDLAPERLAKNSSAADSNALNKLNSVFVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFD7</accession>
    <name>A0A9W8YFD7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein mL50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_001705</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL50 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFD7"/>
    <dbReference type="OrthoDB" id="6220758at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018305">
        <property type="entry name" value="Ribosomal_m50"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10501">
        <property type="entry name" value="Ribosomal_L50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="383"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="562"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="390"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="403"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="563"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="602"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4376421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="620" mass="69249" checksum="140F154E3F081F79" modified="2023-11-08" version="1">MRQIARPSRALGPSHSLCSSLQPRALPWTCRHARHPSLAFSTTAPRTFLLSGKQDKKKHQQFVRRWQKRLLGDSEPIGAHVDPYDPTSPVRIAPEEQGEYEEVLAEDGRDDAEFPLYTPAESDRGLLHVGNEPWLRQKLEGDMAKEFEKLTLRTYTPLTLDMANEVEELTGTPYTLKDENLMMAQTVHNVTGRPYTALNFGLHKKTTSPYELRSRFAQAVAEVYALKEAGLSMDLSKFPNRGIYDAPQWIGDIKLSKTEGGELTLAYPADASAERFLQVMQAAPEWEPTLGEEGEELLTDEPAPDALLPQEQPPVMDPDTPAFKRAAVVKTDPDKKPFDFMSNRPVPRTKPAQEVEAEEVIVTQERALSEEIPTPSRVAEITSAPAESELPSSTLETKAQPSADDRLTSREDLKHGQVESSTPRADEAKWRQVPIADVAVKFALSKRLLQLTGLHISDPHLTSAVTLGDLYGYLCATARPQPTSLYSAIHIEGQRARERAKQQQSAPEVPSRRRADLGDLINLGNVELRRVKPTKTEQRTKTGLDKVVQYALWERGLLDDGNTPKTAASYTKSKSRVVEGTRQAPEFGKPMSSKGANFLAKRTKEWREEQERQARASLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFU2</accession>
    <name>A0A9W8YFU2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carboxymuconolactone decarboxylase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001806</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFU2"/>
    <dbReference type="OrthoDB" id="9998495at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34846">
        <property type="entry name" value="4-CARBOXYMUCONOLACTONE DECARBOXYLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G11590)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34846:SF11">
        <property type="entry name" value="4-CARBOXYMUCONOLACTONE DECARBOXYLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G11590)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxymuconolactone decarboxylase-like" evidence="1">
        <location>
            <begin position="42"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="195" mass="21146" checksum="1E06A4FE9BD30765" modified="2023-11-08" version="1">MRVPYAPSEPPNEEARPVYERIAERRKPRPLIPLDLALLHNPAMADGWSSFIGAIRTKTSVPEGLKELAISRVAVLNSAVHEWDIHAAIAVKAGISRDAMQAAFDLPVTKKATVQKEGVQGLTGEEYAVLCYTDQMTIGVAVEDDVAGKVKEFLSDKQVVELTATVAAYNCVSRFLVALDVGECNGRGMKAVKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG06</accession>
    <name>A0A9W8YG06_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG06"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="190"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="309" mass="35359" checksum="559FB6D46271355F" modified="2023-11-08" version="1">MSSQGTTRVEDFVFDHPAGLRIHLIDTPGFDDTNKSDTDVLKDIANWLAQSYKRQERLSGILFLHPISLNRITSGALRNLSMFKKLCGEDNFASVVLATTHWDEVNPQVGAAREHQLKTTTGMWEDMIKGGSQYLRHDQHAESAQGILDYMIERRQVFTLAIQHELVEEGASLLTTQAGQRLAADLLATEERHRQDLIKIQKNYEEAQRQRDSGALVQYQRLKRETQLQVDGEKRARLDLQANLDTLKREKQDEIRRNQETTARQMAEISRVRMSHTQDQEQMEGQIRDLQVQLESLGRKNGAQVGEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG85</accession>
    <name>A0A9W8YG85_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003123</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG85"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4374382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="206" mass="23229" checksum="B3B7D6EA792330C1" modified="2023-11-08" version="1">MEETTRELKVDDDVDEIAIADDPEELDVAEELVEAEELVEAEELVDAEELVEAGELAVDEMRVVERIVVDGRTVDEDALVAAVDVGVLVLTVEDLVLDRTVDEEQIVDDLVLERTVDEERRVEEERIAEDERIAEDERIAEDERMVDEEEAVERGRAVLHGVEERAGDDLVLTFGVEPAITAEIVVRAKRTQKESRRIIDVLLSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGB9</accession>
    <name>A0A9W8YGB9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_000044</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGB9"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047146">
        <property type="entry name" value="Cyt_P450_E_CYP52_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287:SF5">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="401" mass="45672" checksum="A155502274AFE69F" modified="2023-11-08" version="1">MAFGLAKSWYLDGIVPYAAKRTREFGHTLNIDILGMHLIYTEDPENIEAIMSTKFSEYGKGRDLYRIWKNLMGDSIFATDGSVWEASKNMMHPHLTKARTTDLDYSEEHIQKLLKRISEAGHTADMSDLLTRFSLDLVTHVFLEESANSLEIRDFPLNNAMDQLQAFNTIRTIFKQGYWGSLVPDIFVRSHMLELNKYLDRRVDTILQNSKSTNKGSPRLVDSLRLKCQSKKEIKDQVVALLIAGRDPLGLSLVWALHELSLGFNIRTAIEDTSLPVGSGSNNREPVGILKGTQIIYSVIGMQRRSDIFGDDATVFRPERWENWSPSKWEYAPFNHGPRVCLGQSFAISHMRYALCRIFQEYQAIAPAIPNEDEKGMRLELNIKPASPVICIFKPRGVSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGJ8</accession>
    <name>A0A9W8YGJ8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Dual specificity protein kinase yak1</fullName>
            <ecNumber evidence="13">2.7.12.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">YAK1</name>
        <name evidence="13" type="ORF">N0V83_001713</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="13"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGJ8"/>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14212">
        <property type="entry name" value="PKc_YAK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000087">
        <property type="entry name" value="Dual specificity tyrosine-phosphorylation-regulated kinase 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000380">
        <property type="entry name" value="Serine/threonine-protein kinase ppk15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF17">
        <property type="entry name" value="HOMEODOMAIN INTERACTING PROTEIN KINASE, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="344"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="690"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="803"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="838"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="143"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="812"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="373"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4376429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="900" mass="101014" checksum="CC20A1A325E0A406" modified="2023-11-08" version="1">MEQQWAAYSEAPGATRQARYAPHNMASPQHAQRDASMPAQAKQDPSYASPTAPSRQGSMAMQSPNGPASRGPDYNDGDGDVAMEDADPYKPKYTTSRPNHQHRHSQQLLQHEESAAARRYSPMNLSPTSPYPGSTQQGGPNYTTFSPQAQAQTQTQSQSSRQSPTRTNPYMSPPNNYYSPPSSRPHAPQLPPIQSNMSPETFYPQSATAQLNAVYNREARSPRTSTNPNPPQLPPIGRGPVPKFDKVANTAELQPKINAQPPFRRANPEGGFISPLQALTAHLPMTYKLCNGGFNYQSSRNPRRVLTKPSKGVKNDGYDNEDSDYILYVNDILGSEESGHKNRYLILDVLGQGTFGQVVKCQNLKTQEVVAVKVIKNRTAYFNQSMMEVSVLDLLNKQMDKNDDHHLLRLKDTFIHRQHLCLVFELLSVNLYELIKQNQFRGLSTTLVRVFAQQLINGLALLGKAKLIHCDLKPENILLKNLESPIIKIIDFGSACDERQTVYTYIQSRFYRSPEVLLGLPYSAAIDMWSLGCIVVELFLGLPLFPGSSEYNQVSRITEMLGLPPHWMLEMGKQSGEFYEKIQDEYGRKQYRLKSMEQYSREHGTKEQPSKKYFSATTLPDIIKNYPMPRKNMKPNEIEREMANRAAFIDFAQGLLNLNPLERWTPAQAKLHPFITQAKFTGPFVPPMTLKSSSSRSPAPGVQEQQRFEGMSRQRQQAQQQVVAAQQAQAAQNAAFANMQMNQYAAAQSPHTQPNMYNNMYSNQAAPPPYPAQAAAYGQQMNMMQPQQPRQYNQPQNLYAQATTRAGRQRASTMDQQQSGQSGIPPALQRVISHLDPNAPIRLQPSPAYYPPPPDGAPESAGSARRRGSRAGNSARHQNFVRNLEDRSLEEGYMTQNHWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGL1</accession>
    <name>A0A9W8YGL1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">amidase</fullName>
            <ecNumber evidence="3">3.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_000406</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a monocarboxylic acid amide + H2O = a monocarboxylate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:12020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35757"/>
            <dbReference type="ChEBI" id="CHEBI:83628"/>
            <dbReference type="EC" id="3.5.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.4" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGL1"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072:SF4">
        <property type="entry name" value="AMIDASE C550.07-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072">
        <property type="entry name" value="AMIDASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="7">
        <location>
            <begin position="97"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="5">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="245"/>
            <end position="248"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="560" mass="61500" checksum="0474FC4C7D97A76A" modified="2023-11-08" version="1">MTATPLQLAVVQPKQVPQGTPDFEQKRSAILEAFAKTVPSELRLPADLVASPPLDVSKIPSTCGILTPKEVDITEDYDAVGLAEAIAARKLTAVEVAIAFSKRAIIAHQLTCCLTQWFMGDAIKQAKELDEYLEKNGKTIGPLHGVPVSIKEHMPIAGTYSSQGSFASVVFDEKDSHMVSILRRMGAVFYCKTNQPQLIMHLETTSHYGRTLNPFNIHLSSGGSSGGEGALVAMKGSVLGIGTDIGGSIRGPSAFCGIYGFKPTSYTMPMKGFLAHAFAAELNVLCSTGPMCRSMKDMDLLMRIVLSAKPHLVDPKLVPIPWTGLNTSITKPLKIGIIQNDGFIDPQPPVKRAIAWAQKQLSDPKYSDLFQVKQFKPYNAEEAWDMIRRIYWPDGGQGAKDDVISSGEPVLPLSDWIWKEAAPHGMLNADGVNQLRATRDDFRFKFAESWNEQDVDIVIGPAFVGPACAHDTAFYWTYTSLYNLVDYPGVVVPTPIKAEAKEEYAPDYKPLSEACNHVKELWESSNFEGAPIDLQINARKYHDNELFGALTVLKDVLELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGS4</accession>
    <name>A0A9W8YGS4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000465</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGS4"/>
    <dbReference type="OrthoDB" id="498204at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF185">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE SUPERFAMILY (AFU_ORTHOLOGUE AFUA_3G02360)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="2">
        <location>
            <begin position="9"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="433" mass="46021" checksum="C3093EAB1BE5F093" modified="2023-11-08" version="1">MSSSSTNTVILGAGIIGLSTAYYLSESGNTDPKTIHLIDSSPKLFHCASGLAAGFLSADWFAPSVSSLGALSFKLHADLASAHSGRATWGYAQSTGISLSQDSESAVSGSGEDWLESGASRASVSNHNRPWEEEAAGPPWLRRTKEGIVEVISKDRTAAQIDPLRFCEWLLQRCSERGVQIQQPARAISVSRDSTGILNGIRISKDGAETERKAPSRPADITTLTLIVSCSRLVITAGAWSPRVFTTLFPSVKTRIPVSSLAGHSLLVRNPHSNSAEPDKEVCHAVFATDTLGFSPEWFARIGGELYLAGLNSTSIPLPDVATDVQASEKAIEQLKSCAKVMMLDVDGKDFEYLREGLCFRPITSSGRPIVSRIPDDKLGNIKTRGGGEGGVFIAAGHGAWGISHAPGTGLVLAELIEGRQTSAKIEALRLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGW0</accession>
    <name>A0A9W8YGW0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">DBP2_1</name>
        <name evidence="15" type="ORF">N0V83_000244</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGW0"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17966">
        <property type="entry name" value="DEADc_DDX5_DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 10 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="14">
        <location>
            <begin position="118"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="149"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="339"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="504"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="9">
        <location>
            <begin position="118"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="504"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4377419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="540" mass="58801" checksum="A2EF4B72907454C1" modified="2023-11-08" version="1">MSYGGGYGGGGRDGGRGYSNGGGYGGSNGYSGGGGGGYSNGGGFGGGGYGGRDGGDRMSQLGQGLKQQNWDLDTMPKFEKSFYKEDPAVTARTADEVDEYRKEHQMTVQGKNIPKPVTTFDEAGFPSYVMNEVKAQGFAKPTAIQAQGWPMALSGRDVVGVAETGSGKTLTYCLPAIVHINAQPLLAPGDGPIVLILAPTRELAVQIQQEISKFGKSSRIRNTCVYGGVPKGPQIRDLARGVEVCIATPGRLIDMLEAGKTNLRRVTYLVLDEADRMLDMGFEPQIRKIIGQIRPDRQTCMWSATWPKEVRQLAADYQKDWIQVNIGSMDLSANHRIQQIVEVVSEFEKRDRMAKHLETIMNDKDNKILIFTGTKRVADEITRFLRQDGWPALSIHGDKQQNERDWVLNEFKTGKSPIMVATDVASRGIDVRNITHVFNYDYPNNSEDYVHRIGRTGRAGANGTAITLFTTENSKQARDLVQILSESKQQIDPRLHEMARYGGGGGGGGRWGGGRGRGGGRGGRGGWTGGNSDPIRNSRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH08</accession>
    <name>A0A9W8YH08_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_000587</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NmrA-type oxidoreductase family. Isoflavone reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH08"/>
    <dbReference type="OrthoDB" id="419598at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05259">
        <property type="entry name" value="PCBER_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051609">
        <property type="entry name" value="NmrA/Isoflavone_reductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045312">
        <property type="entry name" value="PCBER-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47706:SF7">
        <property type="entry name" value="CIPA-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G01630)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47706">
        <property type="entry name" value="NMRA-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="4">
        <location>
            <begin position="35"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4377757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="347" mass="38282" checksum="C0B7EB91B0B77318" modified="2023-11-08" version="1">MSTEHKQVDYIRNVALVGVSFPTYSPSHHPNNPQGSGQIGKHMLKHLLAGGKHTVTVLTRSDSKASFPSDANLKVAKVDYSSEESLVSALRGNDFLIITLSVVAPPTQHGEIVAAAAKAGVHCIMPNYYGFGLGERSGKLTSDPLLSSFGRFLADVKKHEELHYVALCCGFWYEFSLAMGEPWYGFDVKNRTATFYDEGTRKINTTTWEQCGRAVAALLALPVPKQEGGKLAVQDWENQGLHVSSFLISQRDMLDSLNRVLGTTDKDWTITYEKVGERYKKGMEELQSGNRTGFAKAMYARLFFESGEGDYETGHELDNGKLGLPKEDLDEATKKAVEMVEGGFGYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHX7</accession>
    <name>A0A9W8YHX7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein mL60</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Component of the mitochondrial large ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHX7"/>
    <dbReference type="OrthoDB" id="2332379at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016340">
        <property type="entry name" value="Ribosomal_mL60"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28271">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L31, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28271:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09784">
        <property type="entry name" value="L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002216">
        <property type="entry name" value="MRPL31_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR002216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="110" mass="13313" checksum="FDA01561BAA80884" modified="2023-11-08" version="1">MFGPFRLTNPLSGGLLWKIPWRLSRHQKYRHRERLRHVDGVVSTLDTALRRMGQSMKKVERWKVEMPTEQEMLPKDKYTVFDRKVKRYRKGIHKVPKWTRVSQRLNPPGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YI59</accession>
    <name>A0A9W8YI59_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001334</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YI59"/>
    <dbReference type="OrthoDB" id="409136at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="391" mass="42926" checksum="2BA9AC6FA2BCF9BB" modified="2023-11-08" version="1">MGQAQEKFISVELEILEQLRAEFTDSYSSIVHFHLYMIGRTAELATPTIMVFCQEKEPRKKAKKALEEGRIIEKLPGFRIGHQSTQPLVGSLIQPATERSAMDQLGLPGSAPRVYFDPSHAIQAFGMPIFVKHSDAILRQATANAVFDGEKCVYLTVSHVFHQNAESVQNTTTDSDSEKDFGSGTEAEVDDVCMEVTSRASMSSAGESLDDGPDSASSESESSTWTNEPSLTQQLVSDNPRASALQMVRATQPMGQGLSDSPAITSIPPIESLEYLGCLTRSSTELDWALVEIKNPKSYARIHEIKSTTWSDNIHTNPQELTLGSRVIANTSHGSIYGILSDDASYMRFPGSFTFQKVYSVALDAPLDWGDCSLFPMSLQLFRMAMLLPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YI71</accession>
    <name>A0A9W8YI71_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Guanine nucleotide-exchange factor SEC12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_000910</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Guanine nucleotide-exchange factor (GEF) required for the formation or budding of transport vesicles from the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Golgi apparatus membrane</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the WD repeat SEC12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YI71"/>
    <dbReference type="OrthoDB" id="16538at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003400">
        <property type="term" value="P:regulation of COPII vesicle coating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045260">
        <property type="entry name" value="Sec12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284:SF0">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 10" id="KW-0813">Transport</keyword>
    <keyword evidence="2 10" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4378079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="365" mass="40354" checksum="BF54A3949F4E5A7D" modified="2023-11-08" version="1">MVSIKNSITLFDFTSRAPNVQPSAELEVSKDDSVTCLAALGTQNGLVLYAGNNSSEEDRSRARNEHFRSFEVQLPKNKRASDAAEKTEASIGVLGKCMLFSAPHSAVAVKEAYQRLIRLSPPPRAATNTPTKRIGAIASSLGDPSELVIFSATSNRPQGQDIIERISFPKNQEANDVDILALGEGRFQVAYVADYEVHIQNVHYDFDQQKSIGKNERRKVYAVPQPDLAEKKARSKLRCIRWLSPKHLLLLANKPNRTGVELVVVHLYEEGPGSVVQRKTLPKHVKAATDMDVALLDADMSGAYQIAIAIGAIDVSLSVYTVDYYGDAQDSLSKIHSFNSYDDVRIKSSIYLQNILILFRSTMCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIF4</accession>
    <name>A0A9W8YIF4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIF4"/>
    <dbReference type="OrthoDB" id="5153521at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="168" mass="18821" checksum="98C26292C09069BA" modified="2023-11-08" version="1">MSTSQTFTGDLRSDAKEFNRDKNSRVKWAHGQSFGPGRREEDGRPRPAKIVPHIKSTYPGHMKARSVFNKDMASASGDSSADEDVEHPSTAPDAEIAYSFDATRGPSHGSQILNHALEQAIERFETKETDKLIKNEYEVLDIDAEEALSPRHRRTVAAEDEEYEFVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJ29</accession>
    <name>A0A9W8YJ29_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Apple domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_000270</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJ29"/>
    <dbReference type="OrthoDB" id="3793367at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57414">
        <property type="entry name" value="Hairpin loop containing domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040909866" description="Apple domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="3">
        <location>
            <begin position="29"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="3">
        <location>
            <begin position="178"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4377445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="257" mass="26614" checksum="97B201ED7A215EA0" modified="2023-11-08" version="1">MHSFTAVIAASALLSAVSGAPHFVRSNTCGSAPTGTSTSNQPLKQLTTCQTAADCQTQCDANTSCQSFCFGLIDNVIQCKLYSVSAASIPKQSSTNLVAYDKACSAVPSVVPTASNPTGKQVDSSSSSSGSKTTEQQGKESQKADSSKTTEQQATESKVTQKVNKKSTTNKQTSANTCGSKPAANTSNQPISTPTVSSEDACKTKCQADSSCKSFTCGEVNNQFVCKLYNVEASKIPAPTTTAQKSALKAYDVGCSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YK69</accession>
    <name>A0A9W8YK69_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pantothenate kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_000607</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YK69"/>
    <dbReference type="OrthoDB" id="498611at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004594">
        <property type="term" value="F:pantothenate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd24123">
        <property type="entry name" value="ASKHA_NBD_PanK-II_Pank4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000115">
        <property type="entry name" value="Pantothenate kinase PanK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.510:FF:000005">
        <property type="entry name" value="Probable pantothenate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004567">
        <property type="entry name" value="Type_II_PanK"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00555">
        <property type="entry name" value="panK_eukar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280:SF20">
        <property type="entry name" value="4'-PHOSPHOPANTETHEINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280">
        <property type="entry name" value="PANTOTHENATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03630">
        <property type="entry name" value="Fumble"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4377777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="462" mass="50812" checksum="B534392138E2F494" modified="2023-11-08" version="1">MSQPPAADLDPATGETGNKGSRPQSADVSSNLETGRPRRTTVQSDIQDILHPGNVRINVQGAFIVDEEQPGTPQSEDYEHDPKDIRLPNHTAVVSHVAVDIGGSLAKLVYFSREPGDDSIGGRLNFLKFETDNIDACIEFMRKLQADHKQNNGSRSDELCVMATGGGAFKYYDRITEALGVEVIREDEMECLIIGLDFFINEIPNEVFTYSEESPVTFTPHPPDPPNIYPYLLVNIGSGVSMIKVSGPRQFERIGGTSLGGGTLWGLLSLLTGARSFDDMLRLAENGDNSTVDLLVGDIYGQAYNKIGLKSTHIASSFGKVYKQKRAAERDAEDGCNGDLKRREDAEHVAGSSELPHVPNPFRPEDISRSLLYAVSNNIGQIAYLHAEKHGLPRIYFGGSFIGGHAQTMNTLSYAIRFWSKGTKQAYFLRHEGYLGSVGAFLKRQPRNWGRRESFVELKGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH27</accession>
    <name>A0A9W9CH27_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH27"/>
    <dbReference type="OrthoDB" id="4148174at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040721515" evidence="1">
        <location>
            <begin position="20"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="308" mass="32921" checksum="03DAEAE36B4AFE2F" modified="2023-11-08" version="1">MRSILSAATILSLPFFAVCQSGSDGYSGYKLDIRDDGDPTAVLYETENTASNVSALSPVPDVMLNASVHVGEIFIGVDNLTAKINLDAQVLQLLSFNAGVDLSIDKVSLLIQNVTAKVYLEARLGNLVTMISDVLDSIDLNPVIAELGNGLGTIINDTAGLVGNIGDGLTGNNETAQAKAKRDLDLHQWDLHNNILYSINNFEGNTNKRRVLAQNGDIVEQTLNNNGLISGSTVVGDYLKDMRFNGFNESVSFHGVEVWEEEYIYEPFAGLYSISGVFKNKSTGEVVGTQLLAEARGGGSASIGNEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH34</accession>
    <name>A0A9W9CH34_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Intracellular distribution of mitochondria</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">CLU1_1</name>
        <name evidence="2" type="ORF">N0V83_010828</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH34"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="74" mass="8278" checksum="4B94E4642F729CA2" modified="2023-11-08" version="1">MAASNNDASKSEAAKTPSKEQNENGQVEPAEEEGANGQQPDNIFQVTIKLPHEPFEMQMTVRLILHSFHRKAHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH58</accession>
    <name>A0A9W9CH58_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gfo/Idh/MocA-like oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010846</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH58"/>
    <dbReference type="OrthoDB" id="2127032at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000030">
        <property type="entry name" value="NAD binding Rossmann fold oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000778">
        <property type="entry name" value="NAD binding Rossmann fold oxidoreductase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840:SF6">
        <property type="entry name" value="BINDING ROSSMANN FOLD OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G11930)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="99"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="413" mass="45797" checksum="618FC515D6EEAD32" modified="2023-11-08" version="1">MAPPEVLMVGTGEYTTGFVGGGASKSDKKVGVVGLTLFDLRRRGKVGKLSMVGVSGGKFPAIREHLQKNISEAYNGLDVSFEEFPKEGKTDPDAFKTAIDALPKGSAITIFTPDTTHYPIALYAIERGIHVLITKPAVKLLEQHQELQTAAKKHNVFVYIEHHKRYDPAYADARFRAQKLGDFNYFYSYMSQPKSQLETFKAWAGKESDISYYLNSHHIDINESMIPGYKPVRVMGSAAKGIATDLGCDPATEDTITLLTDWVKKDDPSKRATGVYTAGWAAPQNAGVHSNQYFHYMASKGEIRVNQAKRGYDVTDDEGGLLWYNPFYMKYAPDEEGNFAGHTGYGYISFEKFIDAVNALNEGKVTLEQLDARPLPTLKNTIATTAILEAGRRSLDENRPVEIVEENGVWSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH95</accession>
    <name>A0A9W9CH95_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycosyl transferase CAP10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_010966</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH95"/>
    <dbReference type="OrthoDB" id="541052at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF104">
        <property type="entry name" value="PROTEIN CAP1, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05595)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="3">
        <location>
            <begin position="561"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="807"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="812"/>
            <end position="834"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4362025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="862" mass="96380" checksum="B4DD59E44A0A79FC" modified="2023-11-08" version="1">MHPVVSLIAENVVKYHQLLAPLLFSTLAAVANVHTPVQHALLVTAVSWLLIWILLIFRVGVYSKGGSGKRRTTWLAGAFLSISQICDRAACDKEGIWATKALLPLFVVLLSDNELIAKHVALPIHTLLDDPAAKSNAPSETHSHTGSYRILATLTVSASVVLSTYTVSTTSTLGFSSVLFAATGLVLFGVALKGTEDDGENNTRELGSANGISPRRNSISGLLKEQQLAALRDVAAIMMTVCGIASYFMEPTMTATTVSWESVYRDYKQDWSTVHNLRILQEVLWMVPVQVFVNAFTFVMDTSTLFPIVHPPSPFEPVPMDLSKGHPAAQLINAAEADFQSTLSRQSQSLADAVREYRRRYGIAPPPHFDKWYEFATRNGVVMIDEFDTINQMLLPFWALKPATIRSRITNALGHEDSALVAVAIRDGAVAHFEGGDEWQQQATIAMMKDFVQYLPDMDLGFNIHDEPRVIVPDVLLSSMVAKARDEVLPRTAKNTVPRNSFSARPEDMNDGTRFTETSITRFNRFAHQQTWTHSRLSCSADTQSQTFEDYAADNLTAFTASPLGYIYNTTAFSDICNSPSLSSTFGFFERPNAFNIIHELTPIFSQSKISSFQDILYPSPWYWIGKVSYDETHDTTWENKTNNLYWRGSTTGGFSRNGGWRRQHRQHIVRRLNAPDKAQVLVPTTTSKPTTANQQADTTTTTTYSVTDIDRRSLKHLINVKFSHIGQCDPGDCDAQNEFFEVTERVDGQDAWYHKHLLDMDGNAFSGRFYSFLKSRSLTYKMAIFREWHAEWLRPWVVWWGGGAGGGGGGKKGEKKSDKGGNGDSAGERKAREIAKRSTEWHDKVLRNVDFEAWLFRLLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIX0</accession>
    <name>A0A9W9CIX0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Glycoside hydrolase 2 (Mannanase, beta-galactosidase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">BMS1</name>
        <name evidence="13" type="ORF">N0V83_009248</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class translation factor GTPase superfamily. Bms1-like GTPase family. BMS1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIX0"/>
    <dbReference type="OrthoDB" id="10260897at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01882">
        <property type="entry name" value="BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000105">
        <property type="entry name" value="BMS1 ribosome biogenesis factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012948">
        <property type="entry name" value="AARP2CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037875">
        <property type="entry name" value="Bms1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030387">
        <property type="entry name" value="G_Bms1/Tsr1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF2">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BMS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08142">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00785">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51714">
        <property type="entry name" value="G_BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Bms1-type G" evidence="12">
        <location>
            <begin position="64"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="410"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="575"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="633"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1116"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="425"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="502"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="575"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="593"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="642"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="668"/>
            <end position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4364651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1171" mass="132947" checksum="C52AA0412C51D605" modified="2023-11-08" version="1">MADQQANRPHRATKEKKAHSGGPNPKAFAYAAPGKLRKQAARSSEVKEKRLHVPLVDRLPEEAPPIIVGVVGPPGVGKSTLIKSLLRRYTKQTLSTPTGPLTVVTSKRRRLTFIECPADSLASMIDIAKVVDIVLLMIDGNYGFEMETMEFLNVLSASGMPGNVFGILTHLDLFRKQEALKLQKKRLKHRFWSELYQGAKLFYLSGVVNGRYPDREVMNLSRFLSVMKNPRPLVWRNSHPYCLADRFLDITPPTDIEQNPKCDRSIALYGYLRGTNFPAGGSRVHIPGVGDLTVTSTEALPDPCPTPFAEKQIEKIGGKKKRTRLGEKQKILYAPMSDVGGVLVDKDAVYIDVKTATFDPEADYSERGLGEQMMIGLQGGRKLLGEDDRGFRLFSGGELVNNKIVDDDVEDTGRRSHRTARAADADEVDDADLDGIDSDDEELQQDHSDDEDAEDDTGFLDTNGASNGKPNLARKEKDVNGDAAEDVAFADSDSDLGSISGDDLDLDDLEDGDVGSENDDEDDDDGAIRWKANLRENAAKLHGKKHPYRVTDLVKMMYNMDLAPEDVIKKWTGNDEEEFEEDEEDEGGFFKKSKTEDSAHAEDRYIPRFDYDELAEKWEDEDNVEALMSRFATGHMNGKGGDDEEEGDDFEGFDEDDEGDGEFEDLETGEKHGGEPPKAEPDSEAKTIEEERERNARRKEELKLRFEEEDREGFMNDKADARKEGGQEEEFGEDDWYDAQKAMIQKQLDINRAEFDQLDELSRVRVEGHKAGTYARIVLENVPYEFSAHFNPRFPVLIGGLTPTEERFGFVQIRIKRHRWHKKILKTNDPLIFSLGWRRFQTLPIYSISDSRTRNRMLKYTPEHMHCFGTFYGPLVAPNTGFVCVQSLSNKTPGFRISATGVVLNVDESTEIVKKLKLTGHPYKIFKNTAFIKDMFKSSLEIAKFEGASIRTVSGVRGQIKRALSKPEGNFRATFEDKILMSDIVFLRAWYPIKPRRFYNTVTNLLHPATPEHAQEGESTSWTGMRLTGQVRHEASLPTPSSRNSAYRPVVRETRHFNPLRVPRKLAADLPFKSQIATMKPQRKETYLQKRAVVLGGEEKKARRLMDHVVAIRNDKVEKRRQKQEERKGGYKKKVAENEEKRGERERREKQEYWAREGKKRGRQEGEWCWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ24</accession>
    <name>A0A9W9CJ24_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_008838</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ24"/>
    <dbReference type="OrthoDB" id="2122982at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16494">
        <property type="entry name" value="RING-CH-C4HC3_ZSWM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039903">
        <property type="entry name" value="Zswim2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21540:SF0">
        <property type="entry name" value="PHD FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21540">
        <property type="entry name" value="RING FINGER AND SWIM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="7">
        <location>
            <begin position="140"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="226"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="204"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4365220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="348" mass="39181" checksum="69BF4B8E0109985D" modified="2023-11-08" version="1">MSSITPNAAQSRTDSDCRLLPARTPRKRKTITYAEEDEDALSDCQLPPDKKQKARDVPAVGDLTTDSPKKTPKKRGKQDSNVVPEEKRLRRFRPNAPQSYLGVKERALTQRLTVLSRERSGTDEVPREKVVIAGSTGNVYTVNISLTPSCDCPHAEKGNQCKHIIYVMLRVLKAQEHVAYQLALLSSELREVIKNAPPIPGVETDGKDGTEKEGEDTNRKPIEGECPICYDELSDKESVVYCKVSCGNNVHKDCMQKWMAMTRGKTTCPYCRAKWAEDTGLEGKLGNVDIKGLERNEDGYVNVAGQLGLSGQRDYSTYHQFWVRQHLGHGRGGRRRRDYDYDDDEDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJC9</accession>
    <name>A0A9W9CJC9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Metal homeostatis protein bsd2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_008354</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJC9"/>
    <dbReference type="OrthoDB" id="10003116at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22212">
        <property type="entry name" value="NDFIP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019325">
        <property type="entry name" value="NEDD4/Bsd2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13396:SF5">
        <property type="entry name" value="NEDD4 FAMILY INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13396">
        <property type="entry name" value="NEDD4 FAMILY INTERACTING PROTEIN 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10176">
        <property type="entry name" value="NEDD4_Bsd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="245"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="245"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4365734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="350" mass="37175" checksum="49FC74F472F26170" modified="2023-11-08" version="1">MPSQGYERVAGHDAEDSPVDSPAAQQWPPASPPPSFRSRASSPTHASRRLMSDDPLANDEDRTLADTFDSDSDDDEDGDGRDDRQRLMRGNPRSDDESPVAPGIQRRVTELPVFNTQAPSSGRVYGGGNGGVWANLSAKPTRGEDAEEKPPTYEQAAADATPPYWETTILAPGTFGDEVFVEGLPVGSLFSFIWNALISMSFQLVGFLLTYLLHTTHAAKNGSRAGLGITLVQFGFGFRSAVLSPGNGSNDNPGPGFDGGVPSDPNAHDFDPDTVASPSSGGGDMTPAASAITGSDWIAYGLMIVGWFILIKSVSDFVRARRHEQLVLQSPDRGLGVAVIAEGESPERSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJS6</accession>
    <name>A0A9W9CJS6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7727 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008039</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJS6"/>
    <dbReference type="OrthoDB" id="2110422at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056144">
        <property type="entry name" value="DUF7727"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40629">
        <property type="entry name" value="PRO41 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40629:SF1">
        <property type="entry name" value="PRO41 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24853">
        <property type="entry name" value="DUF7727"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7727" evidence="2">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="146" mass="16282" checksum="D59D54A1CB221F42" modified="2023-11-08" version="1">MGKLIKNHWARLVILTAAAYQIAAALEAFFWPKIFWDFLTKNLDGAVKPIPVLQILNMILGFIGLAWEWPLPLLAGTGLHRSIEARLIIYPLSALCALLIYQGTNSGLYYIVGMGAYFWAYSEGEVVCPQPWTLPKRGSTARRMGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJT9</accession>
    <name>A0A9W9CJT9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_006944</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJT9"/>
    <dbReference type="OrthoDB" id="431626at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000373">
        <property type="entry name" value="Importin beta-5 subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056840">
        <property type="entry name" value="HEAT_IPO9_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF9">
        <property type="entry name" value="IMPORTIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25018">
        <property type="entry name" value="HEAT_IPO9_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="6">
        <location>
            <begin position="23"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="937"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1007"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="942"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1015"/>
            <end position="1026"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4367363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1026" mass="113887" checksum="E29608336EAEAC48" modified="2023-11-08" version="1">MEDQLVQLLSATQTAQEGPRKHAEQQILSYYGAQELPLGLVGIACHDSVPINIRQAALFSLKNLVLAGWSDSFDEFKGQILVTDENKAQIRQRLLQLVTTDQLDRKLKAAAGLVVSKIAIADYPIEWPDLLDNLLQLIPNATDGQLHGALKVLSELVEDCFNENTFFGVAPRLVKILYDVAVNEQRKPTLRALACKVFHGCFDILEMVLEDHKTTVKSFADEILQEWIPFFINVMNTRLPDPPSYQEEEHDSPNANLYKGHVAFKLQVVKVLMRVRSVFPAILSPQSLVLFQATWEELSLLQPAYSLMYIQEERQSRLEDADGLPYTLDFLVLEELDFMQACLRAPPVRAQLEQELQNQTPENSWVTQVMKLAVAYAQITTEEEGLWDIDVNIFLSEETSVTANYTPRTACGDLVIKLGEWLTEPTINGLLTYTRALYSESKGWKAEEAALYVLNQLLSDFQDVDKQIGPEAASGYVDFIRYAMQQPDAFLRARGYLVAGSLTRTSGDALQQLATSFLEASLQAIPNDESDVVQVSCIRAMQYYLQALPHAVTQPLQSNIILAISNYLAAQDLQELADGDDLMVTLVETLRDAILIDTRICITDNGLDTLFRVASYQANNFQLTMLVVETFEEVTQSISQMGGDGYIQLCAKVLPSLTGAFDVGSLTEENALCNFAADLLAVLAEHGPEPLPAGFVATTMPKLTRLLLGSTDEELLKSATIAVKNILSHDHQQLFEWRDDTGKAGLEVALIIVSRLLSSCSDHAAGEVGVLAAEVVEKAGHERLGPYLEELLRSVAVRLARAEHAQFIQSLTLVFARLSLNHASEVVEFLARQDIEGQNGLQVVLAKWLENSINFAGYDEIRQNVIALSKLYDLKDPRLNQVQVKGDLIPNTDGRIMTRSRAKASPDKYTMVPAPLKILKVLVVELQSASGAPLDASAAAELADEGSDDGDWEDEPNPFVDLSSGFSREQLMAFAAEEPGSGRQRDDETQGFLVDFFKRAATTHEFPDEFNQLTEEEKQRLRDSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJU6</accession>
    <name>A0A9W9CJU6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Multiple RNA-binding domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">MRD1_2</name>
        <name evidence="5" type="ORF">N0V83_008099</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJU6"/>
    <dbReference type="OrthoDB" id="439639at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="67"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="181"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4366463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="373" mass="41178" checksum="CAA5D35D32C329B9" modified="2023-11-08" version="1">MYVADAVMSTVADRMGISKAELLDPTSSDAAVKQAHAETHIIQETKSYFAQHGVDLEAFQRSAKGDLAVLVKNVPHGATPDELRKMFEEHGTVTKFLMPPTGMTAIVEFSNAAEAKTAFMALSYRKMKDSILYLEKAPKDLFKEGLVVSVPQATPSDKPGAKLSATDLLEEAAEPEATNTATLFVRNLNFTTTTDRLTEAFKPLSGFRSAKVQTKIDPKRGVLSMGFGFVEFNSPKTATAALRAMDGHDLEGHKLQIKGSHKGADAAEERRKDDAAKKAASTKILIKNLPFEASVSPFITFIKRVLIKVTEKGCSRVVHPLRPTSVCQGTQEVRFIFAWFWFRRVHHETRRRKRDECLEEHPSVGPTSGSCIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJV5</accession>
    <name>A0A9W9CJV5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJV5"/>
    <dbReference type="OrthoDB" id="3520229at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="153" mass="14806" checksum="5328A5EFF285BCC7" modified="2023-11-08" version="1">MGSSCSTAGFTQFPTSDVACAVGSTSGIPSNTSDVLSSCCKSAPVEQFNGNCGYYCLSVGQSVADLQKCFMDGGVSPSTIFCSGNNTATATGTPSATSSGAKASGSSGSTTSPSGSAQSTGAAPVVFAPQSVSKTGLGMLCMIVVSAFAGAML</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK69</accession>
    <name>A0A9W9CK69_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008058</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK69"/>
    <dbReference type="OrthoDB" id="3695388at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040823228" evidence="2">
        <location>
            <begin position="22"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="476" mass="47532" checksum="C5F9950AAC0515C3" modified="2023-11-08" version="1">MLSFRLLTTVLVASSIANAAALPHQVLDPRTPLAQDDAPPEAEMSTTAAPAGPEGDDPENIFEEFGELLNNHSGYQFLRDLIERLFGIRTEEDGGENEEEATTTTTVFVTPTPEPFSAPPGPSAANTTAEVSAEATPEPSPTDLMSILPVGSMTTAINLTLPEPVFSTGTAIEPESVTLVIPPFPFNATLVGPTATAVPITAEFSAAAAPVSANASTPTTTFQSTTVLLVTATIVPVPVDVSAGTAINTPISDAAAPLWANSTLAAPTAISGTGAALSAAAVTGYSNATAAEAPTYPNSTAVVPVLVSVSGGPATVLDVTVVAPYANATAAAPTPISGTGSPLSAEDASNSTYASPTGFVGTGTVAALSAGTIAPVTFSEDPTYPNSTLVVPVVILGTATTAFTAATISNVPILDTAEAIYPNVTTITSTTTTTISPPSATAPSAAPSLAALALNVTITVNATVPASNDTTLEFPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKH8</accession>
    <name>A0A9W9CKH8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006855</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKH8"/>
    <dbReference type="OrthoDB" id="5397827at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="247" mass="29142" checksum="C790A98305A9D7CC" modified="2023-11-08" version="1">MASLHSIPRFLLPRGPLLLRPQSRALVPLPSPAALRHASSSAKGLSEEFRRRQAAQQSKATPVIPQPDKYRPPSHGKRTPNRNLENRIYGPPISEEDKKRMATKKYPNMMSPEGTFSHWFLNNRAIHLWITMGILVSLAVAAWYMDFISKTIYVSLIPSRKDFLHHPIESSRRFVEVYKMHMEQTSQQYSEQRLKKAEDIEKRKQYRLERQRLAEERGEEYVEDPRYYVGEDGVRRRRVKRWFGIWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKS2</accession>
    <name>A0A9W9CKS2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA ligase</fullName>
            <ecNumber evidence="12">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">cdc17</name>
        <name evidence="16" type="ORF">N0V83_006917</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="12"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKS2"/>
    <dbReference type="OrthoDB" id="206088at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903461">
        <property type="term" value="P:Okazaki fragment processing involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07900">
        <property type="entry name" value="Adenylation_DNA_ligase_I_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07969">
        <property type="entry name" value="OBF_DNA_ligase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3260.10:FF:000004">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000062">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000016">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF4">
        <property type="entry name" value="DNA LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="6 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 12" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9 12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 12 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="15">
        <location>
            <begin position="608"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="839"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="839"/>
            <end position="849"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4367336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="881" mass="97614" checksum="7D59F44FF90DCA3F" modified="2023-11-08" version="1">MPPKQATLGKFFGKQNGAAAKQQSKLAFSTKPKTEDKEVKPKEEPESSPAEAKEAGNGDVESETEVKDELNGEAMEVDMSPSEAGKKRPAKEEEENEDSEDEPPTKRQRRKPKEETAKVKKSKAAPKKADKTPSKVKKPAAQAKIEEEDDEQEATASAKVANGKNAERSPAVEEDAEAGAKEEEDVLSSDEEPEEVKAKARKKVQTTLTSKTKDPYPDWKPGEPVPYAALCTTFSKIEMTTKRLEILAHCSLFLRQVLRLTPSDLLPTVMLMVNKLAADYAGIELGIGESLIMKAVSESTGRSLQHIKSDQNEIGDLGLVAAKSRSKQPTMFKPKALTVEGVRKGLMGIATVEGQGAQGRKVDGIKKLLSAADAHNAGKHVDIEKDKGGPSEAKFIVRTLEGKLRLGLAEKTVVVAVAQAMIFHEMSQNSKTPSTEDLAKAESMLKAVYSELPSYEVIIPAMLEHGIMSLRENCRLQPGVPLKPMLAKPTKSITEVLDRFEGKDFTCEYKYDGERAQIHFVAHSADMELATTAPSAGKSDRGVSNIFSRNSEDLSKKYPDILAKLPTWVKEGTTSFVLDCETVAWDMVEKKVLPFQQLMTRKRKDVKVEDVKVKVCVFAFDILYLNGEALVNKSFRERREHLKEAFIPVEGEFAFAKYGNTNELEEIQILLEDSIKEGCEGLMVKMLDGPESSYEPSKRSQNWLKVKKDYLSGIGDSLDLVVLGAYYGKGKRTSWYGAFLLACYNPNTQNYETVCNIGTGFSEAILETLHTQLSAIVIDRPKPFYTHSTGNKDQPDIWFEPRYVWEVKTADLTLSPRYRAAADEVSGGGKGVSLRFPRFIKERDDKKPDQASTSRMIAEMYSRQESVSKTKGPSVDDDFEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKU4</accession>
    <name>A0A9W9CKU4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_006021</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKU4"/>
    <dbReference type="OrthoDB" id="39734at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056027">
        <property type="entry name" value="DUF7608"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051701">
        <property type="entry name" value="Mito_OM_Translocase_MSP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644:SF56">
        <property type="entry name" value="AAA ATPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644">
        <property type="entry name" value="AAA ATPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G12920)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24581">
        <property type="entry name" value="DUF7608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="918"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="481"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="823"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1116"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="837"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1116"/>
            <end position="1178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1261" mass="137695" checksum="7A7D873E74F57D3B" modified="2023-11-08" version="1">MKQKLTRPLPSQHGASLAPPKAYTGSSLRASTGRPPARFIRGRRYPSIPRLRPFHTTRPLAQAENPPPAPGSTPVDPNAVNVSDREKGATGEPPDAAVATDDPEVLAQKLQRSRETSRRYSAALRRQQRGKKAQGLPPVHIPDWFLKRRVIRHEDLNHGSQHTKPRTVLSVDVSHAESGEHAACSIPAGGDIDPAQVLSRLVRGLWSWRLDDKEKRKVEKYLDEVAALVKKAEATGSLDRSKESMASTQIESGDLGIESHVQKSTEELTLTLRNISDIIEADSLEELNAIKKRTAKLERATRRRELAKARQISTSRRLSPLVVAEIRASIAASLSALRPTVGDSFPSAKTNLILHAPAAEHEKSLDECIYTTALEVGSDVIVLHAQDLAQLAGDYLGEGPEPSPRSIRSLGYETYRLSSDLSNGFADVEEIGEEDADSNQPQSLDQGEPRAMPMHLPIIAISPALRALTQSLKHMHVASTSQFDVPNGAGNEDSGRTQSQSEMQLEDMKLATLLETLIDSNDAKQSRGIIGGEVSLHLSDQSRKSSKGPAFFDYSMTSEGTVLELNSTLPSSAQNEMNLTVKVGAMPRAPEVPKRSKIIYVKDFKEMNATHYGGRIIQKLEEVVRKRRTAGESIMIVGSTCSRDLTPELSANGVRGLQTEGESSFFRTIVIATESSTKETNFEESHAVAMHAIATAELPPSEKSKFRRINLSHIQDMLRSLDPTAAASISDMEQSPLRFRSWAPIFSESNFNSVLTYDEVHRIALTSLGLLVTGPAREAALSNSSPPQVTWAHVALAMGLLRASDTAKYAFFDKVTDLSKRPQNAFKSSDTLDDGEQTEKKRRDKESSQRQRHLQHIASTATKHEKRLMPGIADPDQIKTTFDQVHVPKDTVDALRTMTSLSLLRPEAFTYGILATEKISGALLYGPPGTGKTLLAKAVAKESGSTVLEVSGSQIMDKYVGEGEKNVAAVFSLARKMSPCIVFLDEADAVFASRDAMRERTSHRDILNQFLKEWDGLNDSSVFVMVATNRPFDLDDAVIRRLPRRLLVDLPTQADRKEILRIHLKGEQLDNSVDLDDLAKRTPFYSGSDLKNIAVSAALACVKEENEQAAIAAAKATLEAEPPSPSSSETPPTDASTSSASSSTSTISSNSEPTTPSTQSSTSSSSSSSSSSSSSSSSPSPPPHLVRGQTYSFPEKRTLHARHFDKAIQEISASISEDMSSLSAIKKFDEQYGDRKGNKRRKDFGFGVLAERNESAARVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKY8</accession>
    <name>A0A9W9CKY8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinase</fullName>
            <ecNumber evidence="4">2.7.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_006665</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKY8"/>
    <dbReference type="OrthoDB" id="338650at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000824">
        <property type="term" value="F:inositol-1,4,5,6-tetrakisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008440">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF103">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE MULTIKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4368309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="332" mass="36301" checksum="A439BB867EE0D63C" modified="2023-11-08" version="1">MASKTFDASKLQTFGNAAAGHDGVLSDESGAVVVKPCTAAEITFYESVTASHPDLVPFLPTYMGQLTLSGDQSTVGAAESGTITTADGSVERLHGKKLSTELHIVLENITHGFKKPNVLDLKLGAQLWDDAAKPEKRARLDAVSKVTTSGSLGFRIAGMRTYKGTSAPDVPEDLKEYVESDKEGGYWVYNKMYGRKFSADDVNEGFVSYIFPGSKTEAEQDRAREVLAYFLGEVKDIQEVFEKKESRMYSASILLVYEGDVEEYAKTKQTLRSAQPEEDEDDEANLPKLAAVKMIDFAHATWQSGNGPDENALQGMRSTAKILKDLLDKAQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLI4</accession>
    <name>A0A9W9CLI4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006308</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLI4"/>
    <dbReference type="OrthoDB" id="270167at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112:SF16">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="145"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="455" mass="53887" checksum="297C603E6735CF35" modified="2023-11-08" version="1">MEQLHINQNRHLVCDEDVLFNTHEYLNSLVDKFEISINTQPRNNMNIQTESSTNAPLASNMKDSNMKESKMKENNMKDALSGSWSSFKDWHHTKKAWFDSIETIIEETLVEPDKDQPLELRFVKDLGSYNFELTPPQKVSASTKYITISYCWEQPACAEEIPKYQILVDGSYRDLQCPSHVFHRSVRFARYKECDLIWIDQECIDQQLVCIDRQWINKDLQYHLRVMDRVYSESLCTVAPLSTNAFLKGHSVEHLWSTSFVHFRMDPWFRRAWTMNERFCAREVYLLLKTNWRPPSKGDYSNYEKLEDDLLITLAKVKSASDDIPSDRMSLLLGDKTLRDHARKIITGEIADPEEWRMLYRIRLAMAMCGCKITSDQVTIYGNIFKLPRRLDSNRLNDSKFWLPTCVFALMLANRFPEEEDRKRLYQDEESVLQYWFSQEREDGFGWIRDWHKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLL1</accession>
    <name>A0A9W9CLL1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLL1"/>
    <dbReference type="OrthoDB" id="288942at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051335">
        <property type="entry name" value="Alanyl-tRNA_Editing_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43462">
        <property type="entry name" value="ALANYL-TRNA EDITING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43462:SF2">
        <property type="entry name" value="THREONYL AND ALANYL TRNA SYNTHETASE SECOND ADDITIONAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="284" mass="30281" checksum="AB55C9429848B252" modified="2023-11-08" version="1">MDQRTDPLYQKDGQLHTHTTTITSIHPLSSLPQETQALFKIPPPEEEQGPPYILTTPSTIFHAQGGGQPSDTGTIKSTSEVNNGDGDPNTFHVHQVRKVNTAILHAGTFSPPQAATPFSSNASSEHVRQDIDVAARLLHSRLHTAGHVIGLAIHLLATAPSNPPGGGLIPPDIVDGKASHYPGAAFVECHGLIPGTAKAAIQAKVDELVNADLAVGIHFWSEERARGSVWGCGELKGMRWMGLGLWRLVVVEEGKVDVIRVGDACEEVGECGKVVVRGLRGRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM01</accession>
    <name>A0A9W9CM01_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_005228</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM01"/>
    <dbReference type="OrthoDB" id="10027013at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051052">
        <property type="entry name" value="Diverse_substrate_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942">
        <property type="entry name" value="METHYLTRANSF_11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942:SF4">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="49"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4370707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="303" mass="34479" checksum="B8D948F89FB3BFFC" modified="2023-11-08" version="1">MSGEDIYYKGDSDYWKAYREGRPRVPDSYFERILEYHARNGGEFNVWHDMGAGNAVHTRRLALKFKKTIISDSGASNIEAAKSYLGENSDSYQFRVAKGEEASDLEPGSVDLVFGANMMHYTDIDETLRAVVHQLKPGGTLALGGFGIPLLLDPELQNLWAQVIHRRLETTFESNHEATGLFIVASGPHDAVPFDTQYFRPGALRLKFNERDGWSWRRCMVPESLDGSIPHVKRFGKEDEIIHEDNEDWNFDADVTVMRKILATFPLKPDPVRENLWRQFEAAFGDRKVPATWPSALTLVTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM11</accession>
    <name>A0A9W9CM11_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transcription factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_005432</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM11"/>
    <dbReference type="OrthoDB" id="424974at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052073">
        <property type="entry name" value="Amide_Lactam_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47171:SF4">
        <property type="entry name" value="ACETAMIDASE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47171">
        <property type="entry name" value="FARA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="7">
        <location>
            <begin position="5"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="8">
        <location>
            <begin position="269"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4369670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="424" mass="48262" checksum="691010EFB8CEC777" modified="2023-11-08" version="1">MLESPESAGGDTLVSSSVAQFNALSPRFRKRLEGLTAVHSNNDGVSQELKNGEAAVMRRKELIQEHPVVIVHPVTGEKALYVNPVYTKYIVGFDQEESDYLLGFLFDHIAKRQDLQCRVRYEAGTVLVWDQRVTTHSQTLDYPAGERRHGFRLTPLANKPIPSKVEEDDGDGTPAAGYALAEEGTRRHLVEFIDQEDLRDRPIDKEARISYVGTDVSNINFLVRQTDQSLIVHHFATNRIERRFTAHEPDRIPAEVFELPEKVVVDQLLEAYFEKINPGFPVIDKDRFLKQYQARNPSDPPSLLLLHAMLMVGAHVLEGRDGLEATFFRRAKILIDSRFERNRDVVVQAALLLTWHSDGPEDVAANAWHWIGVATRLALGLGMHRDAEPSTLVEYNKKMWRRVWWLLVQCDLLIALQYGRPLAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMC4</accession>
    <name>A0A9W9CMC4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="3">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_005720</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMC4"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000266">
        <property type="entry name" value="Carboxylic ester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041014382" description="Carboxylic ester hydrolase" evidence="3">
        <location>
            <begin position="20"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="4">
        <location>
            <begin position="28"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4369956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="591" mass="63409" checksum="82BFD2F726190C07" modified="2023-11-08" version="1">MKVLGRALATALLAAGACAEPVPRAAKAPTVDLGYGVYEGSYDANNSINVFKGIRYAAPPLGKLRFAAPQAPAKNKTTTPALSYPPFCPQTGASLETPAAYGFTSAIGNEDCLFLNVWAPANAKNLPVFFWIHGGGYGLFSSTGLDPTEFMTTNNNGFISVVIQYRLGAFGFLAGDDVKEDGALNAGLLDMNFALKWVQANIGKFGGDPSKVTVAGESAGAAAALYQAMAYGGKQKENLFRNVITASPWVPFQYDYDDEQPTESYDSFAEYAGCSNAKDTLQCLRDADTVVLQNASAKVSEAGPFGTFAFLPVTDGSFVKDRFSKQLLSGALKGKRVLSGNMANEGVPLSPPTTRTLQDFRDYITTTFPYFSKSDKFALEKQYSYAGDTQDVNPNATKFCTSGTGYPTALNMSEFGSGQQQRVFNVFAEYAFVCPSYWAAGAFPQGWKYQFSAPPSYHGYDLQALWSGTATPGASFKHAFRKIWGSFIIANSPVISVADAKGGVANSTVPASDASGKIQWPQWDENKPVLLNLNTTGGVAIHVKATEDLQYNLYSDPGVSNVISTADADTWEGGRGKRCDYWESVTAKVPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMF0</accession>
    <name>A0A9W9CMF0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thioesterase/thiol ester dehydrase-isomerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_004676</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the C/M/P thioester hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMF0"/>
    <dbReference type="OrthoDB" id="68328at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03444">
        <property type="entry name" value="Thioesterase_II_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.210">
        <property type="entry name" value="Acyl-CoA thioesterase, double hotdog domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049450">
        <property type="entry name" value="ACOT8-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042171">
        <property type="entry name" value="Acyl-CoA_hotdog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003703">
        <property type="entry name" value="Acyl_CoA_thio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049449">
        <property type="entry name" value="TesB_ACOT8-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066:SF35">
        <property type="entry name" value="ACYL-COA THIOESTERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13622">
        <property type="entry name" value="4HBT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20789">
        <property type="entry name" value="4HBT_3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA thioesterase-like N-terminal HotDog" evidence="3">
        <location>
            <begin position="35"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA thioesterase-like C-terminal" evidence="4">
        <location>
            <begin position="210"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4371459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="37118" checksum="B282FC84F95779D9" modified="2023-11-08" version="1">MSTNPQLPFSEQFALKSLGQNEYETVYRPQRMGNPLNIAYGGYALATACKAACLSVPSGYHLYSMLGNYLGPAYTDRPLHASVRVIRQTRTFATRQIEISQQRSDSSSDEKRVCLIAIADFQVAEPASLLTYSRTPQNHPYPNWKDCPTQQEAFQKLLDEGKISKELLDAQAKSFSLLKNIFDQRAIPSGIFAQNLMGMAKHLPHTQDALPLPRRTTADWIRCKQSLASPTDHVANLAFLIDAAIAFTPLSFSHMFFDDVSAVSSLDFALRVFRNEVDMGRWHLREISTGVGAEGRTYGENWVFDEQGRAVACMSQQSILRPKKEMGKEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMI0</accession>
    <name>A0A9W9CMI0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004971</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMI0"/>
    <dbReference type="OrthoDB" id="5199481at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040916292" evidence="1">
        <location>
            <begin position="18"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="181" mass="19140" checksum="478828D10C6DEC2F" modified="2023-11-08" version="1">MKYILVAAAALCNVASAWPLLPWIQAPPPAGTQFYQLQAKSSVASVANQWVTLASGASSYSLAAAQTAATKFFVNKYNATNTYAFHNSDDTRQLALRGTNGTLLYLVDVTNPNSGNIPGGQLMEWATFTMDNNVLGVNDGSTLKSRTFGVVGGELALYDGVTNTTQGVTPLTLNLVRSPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMQ1</accession>
    <name>A0A9W9CMQ1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004690</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMQ1"/>
    <dbReference type="OrthoDB" id="10251744at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="238"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="380"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="568"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="113"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="260"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="292"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="390"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="406"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="422"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="441"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="486"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="623" mass="69594" checksum="3F6895F1AA73161A" modified="2023-11-08" version="1">MTRTASFSVAPKSEYLRNALHARRAQQHTPTPSPSEGRAPPPPVSRPPEIKTPNASPDIFAEFALTEEQTTPVSPIRRRRPSDVGLPRSKTSRELTNEIEKLKEVLITSNMRVELLKKNNSELQHNMTKANERLEQLEPLEQENYELQDENNHLRLKLENMEDELSRLKETNDDIRGTNEELTAIASESAEHWQGQECAIDEAAECIIKLEEEKTVLSNELRQLKERVTALENSSCSNTLVDGSSARYPSRVHSVDESRPSTSHFDSDYYSQPDSPQVQPSKGSIHSLTPSERSKKFLDLSEERRQSARDLVKRMSAASLKALSLLTSTPAPQAPQIPATFQQKVPTINEDDQVVNSTMKTPGRYRRGRQIVPQSLLDLAQKSPVRASKVVSQSPTTQSEGLRGLYRPDRPTRSRTSDDSRSSSSHVVSPTVTSSVARRQVSATDSTPRASSRGSNKHAHTSSSSEHLQRPSPRHRRQSEADIHSTDSTPRTQTDFGSSEWASLAPPPSVSVVSESDLTTELDPREDKDRWWRSIDRLTLSQVVAQSQGTPQQATDAPPNMILDARFPPGRLDQISSLRSSRSGERKSKTTPNTPFHEGNFLFNDAEDEETFLRKTGIGGSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMV3</accession>
    <name>A0A9W9CMV3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nuclear pore complex protein NUP96 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004843</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMV3"/>
    <dbReference type="OrthoDB" id="3797628at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.690:FF:000003">
        <property type="entry name" value="Nucleoporin SONB, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021967">
        <property type="entry name" value="Nup98_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12110">
        <property type="entry name" value="Nup96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nuclear pore complex protein NUP96 C-terminal" evidence="2">
        <location>
            <begin position="188"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="723" mass="81124" checksum="23D14F294C5C5388" modified="2023-11-08" version="1">MYLPYPKRPKTASDLDYLTGSDRDFHSCSKPHFSEANVLIYGNKGPKILENDIYPTAQEPLVGAEKDTRFAKLPTFTDSNPETLNLQKQHTKIFLTDGVPSARLQTEPASVEFSDLARAVAIGTAEGAHEQHAWQLLSLLFDNADRLPDGVRSEDGEIYRKECLSDFWKHLVWDDAQKHAQQATSLEERAIAQLSCNNVADACHVLLEGLDLRLATMVAQIGGDATMRQSMVTQIEEWRRLDVLSEMEDSHRALYELLSGNCAQSEGKLGSGRENKASTFNISSRFALDWRRAFGLRLWYGIMTEEPIELAVAQFADAISYGTEDVKPVPWFVQENMDMGWNDPQAEHREDLLWGILKLYAASKMDLAANIEDVLAPENISGHPLNARLSFQLFHFFKSRQEDKMEEDERKVGMPTVRGSDAGDHRTSFLSSTTSAAEESQADNPLVELGDKITLTYAASLHTPGHWTTAIWVYTHLSSSAMREHYIRSLLNQFSSTYSIEDTDPTYTYLIRDLHISATWIHAAAALQAKAEGDALRQATHLIKAEELEEAHEVLCRKVGPDAIISRDYDPLRELMGGFLPTPTNSPASFASSARTGSTRRREPVQGWAQGGQIYFDYIELIDLTGQRSTFRVDEDLNTRIQELLVKLQRALEIAGRDRLEACGLEERVALMEIAGTVANLVAKNQHGNRSSILRLPLTEDLWLKHSCDLSTNYYRGVMVSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN30</accession>
    <name>A0A9W9CN30_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_004186</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN30"/>
    <dbReference type="OrthoDB" id="25675at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000118">
        <property type="entry name" value="Phosphatase 2C-like domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039123">
        <property type="entry name" value="PPTC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320:SF24">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="3">
        <location>
            <begin position="111"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4372412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="389" mass="43362" checksum="731AE3749DF2129A" modified="2023-11-08" version="1">MLAPSLRLRSPLQHQLSQAWRHTHQNSRRTPQLSCGAIRCFAKKVSEEQTGAGTTTPVPDDAEPPKQKSPFYFEAGYALFAKRPSRPFPPPFLSRPSTSFSEPLSTHHQSRDRRPTVNGEMIRGVTNGDDAVLVSENFIGANDGVGAWATREKGHAALWSRLILHFWALEVDHASYSSTSKPDPVAYLQTAYDLTKEATTEPNEWHGTTTACGALLASDDHKPEHPILYVTQLGDSQILVIRPSSKEVIFKTQEQWHWFDCPRQLGTNSPDTPTDNAILDRVEIEEEDVVLAMTDGVVDNLWEHEVVANVLESMENWRGNKEKDTEDQTYADGMRFVAQQLVNAAKTIAQDPFAESPYMEKAIDEGLSIEGGKLDDISVVAAQCKRRKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN70</accession>
    <name>A0A9W9CN70_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">WLM-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_004080</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN70"/>
    <dbReference type="OrthoDB" id="261960at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013536">
        <property type="entry name" value="WLM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053000">
        <property type="entry name" value="WSS1-like_metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46622">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE WSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46622:SF1">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE WSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08325">
        <property type="entry name" value="WLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51397">
        <property type="entry name" value="WLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="WLM" evidence="7">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="6">
        <location>
            <begin position="379"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="251"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="312"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="263"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4372306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="52736" checksum="FC8AB570EFDD677A" modified="2023-11-08" version="1">MREIDAHFDAYEHLKHLPRDGEALHMLRKAASMVKPMMRKRGWKVGTLAEFLPDEPQLLGLNINRTERILIRLRYHHDSRQFLSMEQVTDTLLHELSHIVFGPHNADFNNLWNELRDEHQSLLMKGYTGEGFLSQGKKLGGKRIPLDEMRRQARIAAERRKSTTNANAGGHRLGGGPVARGVDMRKVIADAASRRSSITEGCASGSSDAGRLVNQQEQDGFRTTAEQDDANDLAIAQALQDLMYEEEMQRLGAPTGGGGLSWDPQNGLQFDSNPPSRTASPVPSSSQGLGWSKEEGLTFAPATSTQTYTNTHTSLNAQGRPLSRLVTESQNRNTSSSSRRTVSRTSSRLISQEEDALRNKQLPPTPPPTSPQQITVPSDPDKWACPQCTLHNPLDFLACEACGLEQPPQPIPHDKLFGPSHMAPSLPKPPPQSGLRGQGATPFEPAKGRIGWNCLECGTFMENQWWTCSLCGTMKLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNA3</accession>
    <name>A0A9W9CNA3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003205</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNA3"/>
    <dbReference type="OrthoDB" id="5337308at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040956932" evidence="1">
        <location>
            <begin position="19"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="332" mass="36496" checksum="0AE31B31F8620FD7" modified="2023-11-08" version="1">MQLSFSIIAAAIVRTVASGLIYTQSSIDDAVDHTPIVRDLNSNAILNSPRPAQGFDDDDQASAIDWATYTNKGGAFMCGLLATDQSAGQLLRDARTPPSAASLWRGDLKQELSTWYWHVNGRPTYNCKLDDYWYIASVATEAYYEFGINTKGGAIFNFYLESPKFAASSLWYNSRKDADPGDLPQLRALSDILWGYWNRDNPNIKNIRYFFMIGIANPTTNQLIASILHNAGRELSEWPGASFATATDEGHALLGSPNGAAFAYFLMQHKAELGYKIITKVTVFQPDTDDPVDIVDPSLVFHVEEAPEPPAEGAVGTTRDSGAKHIMDQQIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNC8</accession>
    <name>A0A9W9CNC8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">alcohol dehydrogenase (NADP(+))</fullName>
            <ecNumber evidence="9">1.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_003736</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a primary alcohol + NADP(+) = an aldehyde + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15937"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:15938"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:15939"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.2" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4371963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNC8"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008106">
        <property type="term" value="F:alcohol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006066">
        <property type="term" value="P:alcohol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05283">
        <property type="entry name" value="CAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000158">
        <property type="entry name" value="Zinc-binding alcohol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047109">
        <property type="entry name" value="CAD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42683">
        <property type="entry name" value="ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="13">
        <location>
            <begin position="35"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="12">
        <location>
            <begin position="192"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4371963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="352" mass="37404" checksum="FB2FF8D2B3000209" modified="2023-11-08" version="1">MTSPNEFHGWIGHDSKAAQGNLTWSSFTPKPFNPIDVDIKVSCCGICGTDIHTLRSGWGPTHYPVCVGHEIVGHVVRVGSSVTNIALGDRVGVGAQAFSCLKSDCEECSSGLEQHCPKMVGTYNAKYPDGSWSMGGYADYVRVPAHFAIKIPAALKSEDAAPMMCGGITVFSPLRNNGAGPGKRVGIVGLGGLGHFGVLFARALGCESVVAISRRRNKVDDAMAMGATDYIATSEDDKWARKHSRTLDLIVSTVSSPDMPLESYLRLLRTNGTFIQVGAPEDKLPPIAAFGLIGKGCKIGGSQIGSPRDIEEMLEFAARKGVKPWVQRRGWGGECCGWGDGGREGEVSVCAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CND8</accession>
    <name>A0A9W9CND8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CND8"/>
    <dbReference type="OrthoDB" id="2896980at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="445" mass="49968" checksum="0F86F1AB738CBA57" modified="2023-11-08" version="1">MSLSATYLQCFNHNLPQLDASPGPKKTRANPSSPSTANAKPPLVLLCLPTTSFEKRVDQAFQANMDLRTRLQAVRLPTDFPEFALVTEQDVVEATALYILAPIYEVLHALYPSKWLRFAEQKSEKVLNQKGEEEPVRYDLIIKERGGKRKTIAVLEYKKRNMVDYDDFKAALLSYNATPTEIQTRKIAASKLKGRTFLKPNAEAYTKQVSKYAKSTECGNVALFNWDNLVLFDFNRLGQPDFDAGDQANLVWVNESGVGGPFVNGAHIRKALLGFLLKAFEDAELHPRRFFFQKKKKPKDDNAQSESGNGNTGGMVHQGDNPAHQEYGASSRDKNLTTEEESLDQSDPPTAKLGKGRVTLCAMQHDTVDENYQKFKIIIDQSSNVMDIEPVMVLRVHTTRTKIAAVIAIIDPTFKYQADNSPFYLSRDPSLMGKLSKSLLEHYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNN9</accession>
    <name>A0A9W9CNN9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LysM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003644</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNN9"/>
    <dbReference type="OrthoDB" id="2107166at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14273">
        <property type="entry name" value="UBA_TAP-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045030">
        <property type="entry name" value="LYSM1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20932">
        <property type="entry name" value="LYSM AND PUTATIVE PEPTIDOGLYCAN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20932:SF31">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="2">
        <location>
            <begin position="116"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="262" mass="29051" checksum="6D10BD88848FD88C" modified="2023-11-08" version="1">MSEACCTCAALLSSIPPTYNEKTEKPSQFERRLDCCGRAICARCLTDNPRFASYCPYCQIATGPSPLPPQGLRDPPTYSPPDEHSNDELPPYSAHNALPPPPEKSNTGPENSPDVLHFVDQSNDSVRSLSLRYGVPADALRRTNNLFADHLLAARRTILIPGEYYKGAVSLSPRPLEGEEEEMKKTKLRKFMVGCKVAEYDVALLYLEQANYNLEQAVTAYKADEQWEKEHSLEAAKKGKSKASQSPVRRKWGFGGLTGQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNS0</accession>
    <name>A0A9W9CNS0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNS0"/>
    <dbReference type="OrthoDB" id="449487at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050313">
        <property type="term" value="F:sulfur dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070813">
        <property type="term" value="P:hydrogen sulfide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07724">
        <property type="entry name" value="POD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000033">
        <property type="entry name" value="MBL fold metallo-hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051682">
        <property type="entry name" value="Mito_Persulfide_Diox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044528">
        <property type="entry name" value="POD-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43084:SF8">
        <property type="entry name" value="METALLO-BETA-LACTAMASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43084">
        <property type="entry name" value="PERSULFIDE DIOXYGENASE ETHE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="20"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="301" mass="33796" checksum="128516D401513E67" modified="2023-11-08" version="1">MVDNSIMGPSVHSSYEKQTGTWQYVVVDPSSQKAVIIDSVLDYDPATQVISTQTADSLLSLIAEKDYKIERILETHAHADHLTAASYLQSRLEKSQGYKSPICIGKRIMQVQELFGKRYGVPVEEYRDVFDKLFDDDESFEVGNLKATATHLPGHTPDHIGYIIGDNVFCGDSLFHADIGTARCDFPGGSAIALYESGKRLLQLPEHTKIWTGHDYVSEERDTPVPYMSVGEHKERNKHLSRTITQGDFVSQREERDATLAEPKLMNPSLQINIRAGRLPRATPLGHRLLHLPLKLKGVEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP53</accession>
    <name>A0A9W9CP53_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">cAMP-dependent protein kinase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_003281</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8 9">Tetramer, composed of 2 regulatory (R) and 2 catalytic (C) subunits. In the presence of cAMP it dissociates into 2 active monomeric C subunits and an R dimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the cAMP-dependent kinase regulatory chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP53"/>
    <dbReference type="OrthoDB" id="417078at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd12098">
        <property type="entry name" value="DD_R_ScPKA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000039">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000118">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012198">
        <property type="entry name" value="cAMP_dep_PK_reg_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003117">
        <property type="entry name" value="cAMP_dep_PK_reg_su_I/II_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02197">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000548">
        <property type="entry name" value="PK_regulatory"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00394">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47391">
        <property type="entry name" value="Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0114">cAMP</keyword>
    <keyword evidence="4 9 10" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="12">
        <location>
            <begin position="199"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="12">
        <location>
            <begin position="330"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="44"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="406"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000548-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4372990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="459" mass="49946" checksum="E4808AB8D0E2EA54" modified="2023-11-08" version="1">MSLPDYSNEISALDREILKHKPNDILQFCANFFNRRLESQRTEALLSQQHSTQQGAAENSFPGNNPFSSSPSAGGFTKSPMQRLEEEEENDVMTSPTASSFAAVDFGKPRSNNDDAGESAFGNFQGFGGSSKNNITQMPPIVGEGQNFPSNYNTNRRTSVSAESLNPATTANDSFTPPHHPKTPDQLSRLKSAVSGNFLFSHLDDDQSSQVLGALHEKPIPTKGIKVIQQGDVGDYFYVVEKGSFDIYVNPSGKLEAGPDGLGNKVGSVGTGGSFGELALMYNAPRAATVISTDTSTLWQLDRITFRRILMDSAFQRRRMYEGFLEEVPLLSSLTPYERSKIADALETKKYPPGTTIIQEGDVGESFFLLESGEAQVFKRGIDNAVKQYKKGDYFGELALLNDAPRAASVISETEVKVATLGKNGFQRLLGPVEGIMRRNDPSKAGFVGDHVDPLSKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPW7</accession>
    <name>A0A9W9CPW7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">rRNA methyltransferase 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MRM2</name>
        <name evidence="8" type="ORF">N0V83_002269</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPW7"/>
    <dbReference type="OrthoDB" id="20105at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF18">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="7">
        <location>
            <begin position="100"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4375185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="310" mass="34793" checksum="CECE077C059C16A9" modified="2023-11-08" version="1">MLSRRVFKQLTDSFLLGPGYSAAVVPCLAAQCQACRACNRAPAKPIRHASSSSSTRWKSRQGSDFFAREARVQGLKSRAAFKLLEVLCCYSEDCSRADSKQLNEKHKLFKPGQTVVDLVAVNRTTPDGRVIGIDLIPAQPPRGVSTIQGNFLSPIIQDEVRAYVRDPDLGRPRKQITSRNQGRTEEELDEMERGYVDIERQARLDGVEIEPVGQPTEDMGQGTIPDAKLTLKERDIRQGRAVDVVLSDMSEPWDQTAGFYKKSLSDPYFRMMNTSGNAFRDHAGSMESKEAYFVALRRKETPTKEDVFRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPX9</accession>
    <name>A0A9W9CPX9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">N0V83_002437</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="9">Belongs to the NRP synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">In the C-terminal section; belongs to the NRP synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009403">
        <property type="term" value="P:toxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05930">
        <property type="entry name" value="A_NRPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19532">
        <property type="entry name" value="C_PKS-NRPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000019">
        <property type="entry name" value="Polyketide synthase type I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.30">
        <property type="entry name" value="Nonribosomal peptide synthetase, condensation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010071">
        <property type="entry name" value="AA_adenyl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001242">
        <property type="entry name" value="Condensation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013217">
        <property type="entry name" value="Methyltransf_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032821">
        <property type="entry name" value="PKS_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020807">
        <property type="entry name" value="PKS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049551">
        <property type="entry name" value="PKS_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049552">
        <property type="entry name" value="PKS_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013968">
        <property type="entry name" value="PKS_KR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01733">
        <property type="entry name" value="AA-adenyl-dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF20">
        <property type="entry name" value="HYBRID PKS-NRPS SYNTHETASE APDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00668">
        <property type="entry name" value="Condensation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16197">
        <property type="entry name" value="KAsynt_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08659">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08242">
        <property type="entry name" value="Methyltransf_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21089">
        <property type="entry name" value="PKS_DH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14765">
        <property type="entry name" value="PS-DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00826">
        <property type="entry name" value="PKS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="13">
        <location>
            <begin position="6"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="14">
        <location>
            <begin position="931"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="12">
        <location>
            <begin position="2383"/>
            <end position="2461"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="12">
        <location>
            <begin position="3550"/>
            <end position="3629"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="10">
        <location>
            <begin position="931"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="10">
        <location>
            <begin position="1083"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2526"/>
            <end position="2552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="2540"/>
            <end position="2552"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="10">
        <location>
            <position position="963"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="10">
        <location>
            <position position="1143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4375351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="3968" mass="435144" checksum="C11E9E3FA2234066" modified="2023-11-08" version="1">MYNRQSEPIAIVGSGCRFPGAANSPAALWQLLRSPRDILKEIPKERFDVRGWYHRDGDHHGTSNVLHSYTLEENLTKFDANFFGISAGEAESIDPQQRLLLETVYEALEAGGHTIESLRGSDTAVYVGVMGGDHETSLLRDANSLPTYFATGTSRAILSNRISYFFDWRGPSMTIDTACSSSMIAVHNAVQALRSSDSRVAIACGSTLLLGPEMYVAESNMHMLSPTGRSRMWDADADGYARGDGVATVVLKKLSDALADGDHVECIIRETGINQDGRTQGITVPSSEAQAALIRRTYARAGLDLANPADRPQFFEAHGTGTKVGDPKEAAAIHSTFGGLDSDPLYVGSIKTVIGHTEGAAGIAGLLKASLSLQAGVIPPNLLFNRLNPSIEPFIQGLQVPTHAKPWPALPKDAVRRASVNSFGFGGANSHAILESYQAQIDNVSSALVVPATPFVFSAASNTSLVSSLQAHLDYLKTNPVNMRDLAWTLQQRRSTFSHKIAFSAPDVESLIRKIELRLKEYEDKSEANIGVRSSSTPPKVLGVFTGQGAQWPAMGAELIRSSEFVRRRLQELDGALATLPAADRPTWKIADQLLLDAKSSRITEAAFSQPLCTAVQVVLVDLLRSAGIMPSSVVGHSSGEIGAAYAANFINARDAIRIAYYRGLYAKLAGGSTGQKGAMLAVGTSLEDAAEVIELDAFVGRVEVAAHNSPASVTLSGDADAISEVKTVFEEEKKFARLLTVDTAYHSHHMLACGDAYVKALRACKVEVNRNRDTSVTWYSSVTGGEVMEPTTLLQDLYWRDNMVHRVSFHEAVTAAARAEKPTLAIEIGPHAALKGPTQQNIAETGLNLPYTGVMRRGLNDVEAFADGLGYMWTQFGPEAVDFTAFERLFLDAPQAKLATGLPSYHWDQKTHWQESRIARRMRSRSDPFHELLGVPHVDNTELERRWSNVLKISEVPWLTGHKLQGEPVFPAAGYASMAFEAAKSIIGNRPIKLIELRDLVIGKAITFGGESDPAAETLVTLTDITPSKSNSKTRAAKFSVYSAAIKSARDLEVNASGTINVLFGEPSISVLPPIQVDDSNMTETDSERFYTSLSTIGYEYSDSFRTMANLKRKLNQATALISSFDYGDESERLMVHPALLDVAFQSAILAYSAPDDGRLWSLHVPTFFECIRVNPELCATLPLHSVQLPVRAQLLAPEANAVDSIRGNLDILSADEQHAMIQVQGAAVVPVSMATVSDDRTVFSYNKLGVTAPDGELALGELKPSTNDMELGTVRERLAFFYYRKWIHELTEADWTKSEWHFQRLRDSINWTLSLVDAGKHPYIQPEWRTDTQTQIDALFEAHADSVDCRLVRTVGETLPSAVRRQSTMIEHMMKDGLLDEFYKYGLGFQVYNETLGRMVSQFTHVNPRAKIFEVGAGTGGATKFVLETIGNTFSSYTYTDISSGYFEKAAEAFSDWKDKLIFKSFDTERTPESQDFQEGTYDLILASNVLHATESMEKTLKNVRRLLKPGGYLMLLEITNNKLAAFTSMFGGISGWWAGCEDGRPYAPTMPLVAWNTVLRQSGFCGVDSTTPNRDQLAWPFSVISAQAVDNRVNYLRKPLNRSSNAAQISHLTIIGGKSLEVAKIAEDIADIAGLRCQSVASFADLISAQSSVVPGQAVICLSDLEDPMFKSLTPEKMDALKIMLEHSQTLLWVGRDSRDSPYQQAMVGFLRCIIHEMPDLSVQHLDIASGLKGVSTLIMEVALRLHTVSQWEASDALDQQLLYPKETELMFQHGSLYAPRMLPDPTRNDRINSQRRSVAKHASLKSDVISISETGSGLPVLKDVPFFSKGQEGRSAMKTCLSTLSALGITAGLYLFVSVGLEISTSNAVIAFSSSNSNKSYPVATIPTDTREDPAGLLSAIAAELLAARMLSTVVVGAKVLVHEPGTGSLGSSLAQALRRQASARKFEVIFSTSRADDDPSWLRLSPWMHGHNIKRLLPTGVTNFLDLATDDESKEVSALIQQALPGVQYTGSKDLWRATSSGLFQDQEKLISLLKNAATAAGQHVASHKPTELSLSQVADVKIPLHPATLVDWTADDTAPVIVDTIDATPLFSANKSYLMVGLTGQIGQSLCEWMVRNGARNILLTSRHPSVDQKWLDSFKVYGAKVAVYAMDVTSKANVIQVVNDIQASYPPIMGVANAAMVLKDTLFSQMSYEQMTDVLRPKIDGTNWLDEIFYDTPLDFFVCFSSTSSVGGVFGQSNYAAACSYMTSLVRQRRARGLAGSAFDIGRVAGIGYIERASQVVQETFEKYSYMPLSETDLHQMFAETIFAGIPEMNLDPVISTGIERVPADRKHLPPWILYPRLSHYLIDTQATNTVADSKQTVQPVREQLIEATTAEQVLSIIKTCFAAKLQIILQLTGEEMDMNVPLVEIGIDSLVAVEVRSWFLKELQTDMPVLKILGGGSVSDLAEQASKKLPAELVPMLGDTATPSITKAEKEDPGQRPLMHQEVSFVATNASKTERNASLERPVLRQALSFAGTATTTDTHSRGESIATPPSSVGGKSSISDLESIAESKELREFKVLRTEPMSLSQSRFWLLDLFLDDRTASNVAFQYQLNGNLEVDKLAQALELVAMRHESLRTFFVNDASQPDMAVQSVSATNHVKLEHRTINHVDEAAQEYEIHKRHDFDLGKPQLLRMTLLTLNTMTHYLLVAYHHIIMDGISLQVLLSDLEKAYNSTPLGPSPMQFPTFSVNQCKALQAGEMDSELEYWKDVFHDSPAVLPLLPVAIRSSRAPISAFKSNEVSRRVNPVVGQKVKAVARMSRSTNFHVYLTVFRILLARLADVADLTIGIADANRTDDDTTRTIGLFLNLLPLRFKPDEAKTFAASVTETRNQAYTALANSKLPFDIMLQEFHHVERSSTHAPFFQAFFDYKVGVKQISKFGNCQMNMQQNNTGETNYDINITVVEDDQGPGIIFETQATIYDRHATTLLLESYINLLDAFVNAPTMRTGEPSLYSQAQIQRALDMGKGPDLLSQWPATLSSRVEQVARQNKDAVAVRDGNGTSISYGALMRRSQAIAATLEKAGVDPGSKVIVFQQPSVDWIVSLLAIWRIGCIYVPLDVANPLTRLAAIVETCQPSVILTDATTSPNANSLCAANALTINVSNVPAYASAIVPDLSRREQAAVIIYTSGSTGTPKGITVKHEGLRNYIEGSIRMYGMETPQTTLQQSTLSFDISLEQIFTGLATGGSVFMCPLNQRGDPLQITKVMRDEHITYSQATPSEYLMWLQYGMSTLRQCSDWKYAIAGGERLTNTVAKQFAALNLSDVRLINSYGPAEISCGSSFHEIDYRQGEQGDIPIGSQYPNYSAYVVDGDLKPLPIGVAGEIVIGGAGVAAGYANNAALTSTQFVPNPFASTAYVANGWSKMYRTGDYGHFREDGSLVFRHRISSDLQVKIRGLRIELGDIESNIVRAGNGAILEAIVTVHGEDSDFLVAHVVFDTKRDIADKDAFLQNVLQDLPVPRYMVPAMAIPIKHLPLNNHSKVDRKAVKALPLPEHIATPTLSSELTDTETQLFRLWQDVLSTHVTNISITPATTFFSVGGNSLLIVRLQSRIRKELSVVVSVFELINSNTLGEMACKIEESVGVETIDWERETAVPFVAIPTPVGPYTAPRNQPKVVVLTGATGFLGRHLLRRLENDLNITEIHCVAVRDKPVASSPKVSVHHGDLSDPMLGLSQPEFEHLSLTADVIIHLGAVRSFWDNYHALRQTNVQPIRELVKLAAGRRVPIHFISSSGVLPTTAYAPIGDSVAQYQPATDGSNGYVASKWAGERILEQANADFNIPVSIHRFVAGGPEPPRDAVMPVMQWLAQHSEMHKIKPDIAGWRGHIDLMKMNEATEALCESLVGDSPRARFLHHESHVRLRESDMEAFVGVRKGRADLEEMELLDWFGRIKDDGFEYFLSSHMASFQAGDQNGGNVLTMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRH1</accession>
    <name>A0A9W9CRH1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001687</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRH1"/>
    <dbReference type="OrthoDB" id="2264563at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13311">
        <property type="entry name" value="PH_Slm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046868">
        <property type="entry name" value="BAR_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046869">
        <property type="entry name" value="SLM1/RGC1-like_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043453">
        <property type="entry name" value="Slm1_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941">
        <property type="entry name" value="CYTOSKELETAL SIGNALING PROTEIN SLM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941:SF1">
        <property type="entry name" value="CYTOSKELETAL SIGNALING PROTEIN SLM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20400">
        <property type="entry name" value="BAR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20399">
        <property type="entry name" value="PH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="302"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="413"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="421"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="448"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="468"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4376404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="493" mass="53973" checksum="B93ADDD8E5CAAF4B" modified="2023-11-08" version="1">MSAPTSATSELPSRSLTNRSVDEDAIPGEDTSEVTKLFGERLQAWKHAVAYLEDYVTATEKTNHAHGKEYERVLKTVKDPLKEGHHFDQSLGGIAGMFENIRSNTQGISNQHYETAKQLKGTILPIFERLHTEIKNKSKELTKGAGKGSKAVDKARAQTQKHIEMLGQYTATYDSHAGNLKATDDPYILQRGVMHRLNKQVQEENNNRQDLISVQNSFAQFEAHIIQTIQHGMGQFLQVITTQAEHTKAAYGDMIGTTQRVPLDFEWNGFIQRNNTVLIDPTSPARTVSQISFANQNHRSTQPLISGSLERKGKVMRSYDTNYYVVTPSKFLHEFKTDDDFAKDPVPEVSLYLPDCMIGAVNGAKFNVKGKDVSKGKLGGAFSMSHEYQFKAHTPQAANQWWEIIRQAAGTVTNDLPEGSVPTSPVTGNDSPSGATQALHGEEKQPAPLQTQGLQNTDTATADHAPGSTVTPGTAASSNTAPGSAVPGEPGKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY51</accession>
    <name>A0A9W8XY51_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010056</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY51"/>
    <dbReference type="OrthoDB" id="635774at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="359"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="604"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="638"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1056"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="663"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1157" mass="130088" checksum="03B17E6ABBE55D02" modified="2023-11-08" version="1">MPHLLAADNGESSRSRSRQRARTEVGEHRSMWDRLSLQKIGSWLKREEDEPPPLDKAGKHDSEHDKENGRPHSSGEHASRAGGLLNRRSSRRVVPGLPRPLTFKRMNSEKRDRLLEVPQEGEQKRAASADRRESVGKKRHLSPPPISVPSMSAPDILSPHESDGQQKPLPEPTIGGGPDSNIPAGARQVDYGVEDGEDYLSAGELPPVHVTDAYSEHGSHRSDSDIDEIRLQEELEAKWILNLSMHFRDMSDREKFFITYAEEPNKWRRVTVSCDYRQLEPESLEADLKTLHYQRDKSSRIYEAIRDSLPDIQFYDTVTNLKLETTDGRLHVHVTEDVNEIIPYPPTSAIDHLDCKRYREKVVSFDSHISGFVYKVSVNNRAYIKKEIPGPDAVEEFLYEINALSTLQDSKSVIKFEGIIVDEQNELIKGLIISYAEQGALVDMIYDYKPTDQLYWERRERWARQIVEGLSEIHEAGFVQGDFTLSNIVIDHNDDAKIIDINRRGCPVGWEPPELAKLIESGQRISIYIGVKSDLYQLGMVLWALAEQQDEPERQERPLTRTLDRHNTDVPVYFREIIRACLCDTPRGRPSAITLLSRFPEQRELQEPKTKVNSRESVSTHRSDKEYIDPATAVDLEDISNHRRHSRNKSSFENIHIPPTEYPASSGSYIIPNNNAERGRSPHPLRSSSGNRRSDCSPYPGHRSVMSLDDSELENELASLPASRETRWEQVYIDGDTKLVQRGCGEIDVHDFAAQEPKDVCITTPPGEMENSFLSSKTIDDTPLHLSTVAELQPVDPQVTQNVLQEQHEKTHHRGVGSKASFSDRVHQLAQSQTQHTAPIDAMYHINRHSQIPDSQTLASLEHELSMDSSVSSSAAPSRVGTSFSIFDLPERTARVATGFSVATGHHMPLHQDSGFAEPERVSYESERIRHSMGESINDLKLSKEEEDRINERLWDEKDGTMFGEGVCGANLDIKPTTIPVFTIPTAPEKPTIPASSSIAPATAPAALSVGPVDVLRAHDVRDKALEEKVSNTTIRPPSILIPEFHHGVPKTLDATEANSPPIATPATGSSCYEYTTMTTTPESSFHHHHSSSQYQSPDLDAYSNADLNLREELEPKRASDDLADYLSHSISYSQPSDYYHCAAQQETKQGSQEPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYZ4</accession>
    <name>A0A9W8XYZ4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_009762</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SH3RF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYZ4"/>
    <dbReference type="OrthoDB" id="1305878at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000039">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052256">
        <property type="entry name" value="E3_ubiquitin-ligase_CHFR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16079:SF4">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16079">
        <property type="entry name" value="UBIQUITIN LIGASE PROTEIN CHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="20"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="876"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="94"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="262"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="685"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="94"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="120"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="165"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="286"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="371"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="397"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="412"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="480"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="688"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4363468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="935" mass="103942" checksum="EA3A154020A77628" modified="2023-11-08" version="1">MAEAERAGFVQGDLEKELTCSICTDLLYQPLTLLDCLHTFCGACLKEWFAFQASTATSIHPYTCPSCRASVRTTQPNATVTTLLDIFLKANPERGKSAEEKQADREKFKPGDSVLPKLRRRDERDEADRRLLDEVQQLSLREVGIPSGTSSNLEPPRERRRRARSREESRESRASRDERRSTSRNLPGASPSRAVIPPRAVEHQSSLRSLLSASELDSEDIDEDLVRQVLEELVSEGVDLNQIGPAQEDDITERLAEAVRRRQAERHAERHRERRERRDRLAREGLTSSSASSLPQPVRSPLTRDEEAARRRSYGRPESGASTPQTAAGPPISRPGLIDAANRSGRAHHQRSSSQGSSRSAWRAERPAALSVSSVTQGNLDTERPSASESAPAPRRRQSDDQQRGRLDARQQFRNSLQTSASSNPNSPRTLSFNMPASDSPTSSLAIVGGPAQSSSTPAITPFQPPSLRRTTDPARVGQSRPSTTGTPPAISSPTFPRSTTDPHETRPRHPSASTSDVSLVHTFTEPHISCRQCGKDHIEYELHYNCARCDDGGYNICLRCYRLGKGCKHWFGFGWTAWPRYERQAPEGGYPPNHEHPHILVGTRYRRPVTPLTESSTPPHVLMSEDDPEQRLEAGVFCDICKAYANVCYWKCDYCNEGDWGFCNDCVNQGRHCTHPLLPVAHKPTETAQSSADATAPASSAPRFDSGLAPPDHDSTPTTPPLTPKSASVIRGPGYFTIANLTFRPLTFTTLCNICRYPIPPSHTRYHCLKCNAGDYDICMNCYHKANVSNRISREDGLNGWRKCFRGHRMVVVGFEDRDGGQRRIVVRELVGGYALKEDEDEANTNARSQASTSPRLVDDKWKWSSSHRYPPDGGVGLRLVATWRYWPDEGVKDELAFPRGAEVREAADVNGDWFWGVYSGAKGLFPANHVTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYZ7</accession>
    <name>A0A9W8XYZ7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010881</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYZ7"/>
    <dbReference type="OrthoDB" id="6431331at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43689:SF8">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43689">
        <property type="entry name" value="HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="97"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="482" mass="53509" checksum="C20B3EB9BD35F015" modified="2023-11-08" version="1">MAGVLADIGWYGFKLGFGLFSLASIWSSAVIQNGALWARDSEKERQELAAAQQKHWSLDREPLPGFRHAFFKTSNGTRLHYVVNEGVQASAPKNVAIFIHGFPDSFLLWRHILQSPELLRSHILIAVDLPGYGGSDSLPSYGPYEVLEALSEFIISMRTAFLQEDKRVVVVTHDWGALIGARLASEAKELADHWIITSAIIPHLTASNALAKGTLAKQMLHTWIRSPFNIRLLKNGLHALGPVISQFRRSFYIFCFDLPWPFSNFFATFGNYWFLRVLHDLGKGRRNKSDGAHTRLNTAEAAEAMAMSTGPAVDQLEQQEDGSKDRYGESVRKRLVDRGMAEKIRVYREGLFIGSWEKSLETTAALFEMSSDTSRRHSSSSGSLLGNYAPNGALKAPATIILGEHDPAFDRRLALDNVKDYLVKGSQVVVVKGAGHWLPLEQIGRRVLERTIQWALNDSSQSSGGGSTPFAAMSDVRVIEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZC8</accession>
    <name>A0A9W8XZC8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Translocon subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SEC61</name>
        <name evidence="14" type="ORF">N0V83_010393</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZC8"/>
    <dbReference type="OrthoDB" id="420669at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3370.10:FF:000002">
        <property type="entry name" value="Transport Sec61 subunit alpha isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019561">
        <property type="entry name" value="Translocon_Sec61/SecY_plug_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006341">
        <property type="entry name" value="PRK08568.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10559">
        <property type="entry name" value="Plug_translocon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00756">
        <property type="entry name" value="SECY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="75"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="416"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="440"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Translocon Sec61/SecY plug" evidence="13">
        <location>
            <begin position="41"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF10559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4362300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="477" mass="51825" checksum="4AC5CC03A81EDD4D" modified="2023-11-08" version="1">MSGLRFLDLVKPFVPLVPEIQLPESKVPFNNRLVWTGLTLLVFLVMSQMPLYGIVSSDTSDPLYWLRMMMASNRGTLMELGITPIISSGMVFQLLAGTHLIDVNLDLKSDRELYQTAQKLLAILLSFGQAVVYVISGLYGQPSDLGAGICVLLVIQLIIAGLIVILLDELLQKGYGLGSGISLFIATNICESIMWKAFSPTTINTGRGPEFEGAIIALVHLLFTWPNKTVALKEAFYRQNLPNVMNLISTVIVFGAVIYLQGFRVEIPVKSARQRGVRGSYPVRLFYTSNMPIMLQSALSSNVFLISQMLYSRFSDNLLVRLLGVWEPKEGSAQLFATSGVAYYMSPPLSITEALSDPLKTAVFIVYMLVACAVFSKTWIEVSGSSPRDVAKQLKEQGLVMAGHREESMYKELKRVIPTAAAFGGACIGALSVGSDLLGALGSGTGILLAVTIIYGYFEIAAKEGDMAGLKGMVMGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0S1</accession>
    <name>A0A9W8Y0S1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Target of SBF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TOS4</name>
        <name evidence="3" type="ORF">N0V83_009256</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0S1"/>
    <dbReference type="OrthoDB" id="5348546at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="120"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="430"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="368"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="523" mass="57497" checksum="1F45746BCE72B8B8" modified="2023-11-08" version="1">MDSSPSKMLQPSLVESTESQPEQARKPTLLPAFDDAAFSSSPFPRPRSSKRKFEDSPVFPKQELKYYYPTPVPTSSTGILPSSSPRHTRPGLERTVSALSERTPLGAVPTVDLPADGTICRMGRSSNSADYQLSTNRLISRVHVQAAYHAPSASYPQGYIEVECLGWNGAKIHCRGRVFELSKGDTYMSENPETEIMLDVQDTRVMIAWPAISARKLSWDSEEEGMATPTRRRHQANFDSSPPVVPRSPVSQSPIRAPVFTGLASVVQPGPVQVFEDADASRESPSANSPDSADKTFIRPSLPSSRSSQEVHTLDSKASLLSSFAADDFSDNDEENDPIVHSFGPFGQNLLSGLASFNTTTPTQSNTPRLRAPLLSPSPKRPSTESVRFKESPIKNHVINQLVFSRIHSQPLSVIHSNLPAELRGCVPKNVEGKNADDGDSTPAPQLSRADLKKILDETPCVGEIPRAGKDAAGQPLENEFYYVPEMDSNQMRRETITAGTRSTGLRSVRKTHKQYYWKKPRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0V5</accession>
    <name>A0A9W8Y0V5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009587</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Mycotoxin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0V5"/>
    <dbReference type="OrthoDB" id="2018133at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006108">
        <property type="entry name" value="PRK08259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43802">
        <property type="entry name" value="ENOYL-COA HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43802:SF1">
        <property type="entry name" value="IP11341P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0843">Virulence</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="271" mass="29123" checksum="E92B712AFC899DB6" modified="2023-11-08" version="1">MSSSTPEPEPKAVQYKPSPSGIAIITLNRAKQRNAVDPRTAHKLYDTFLQYEADATQKVCVFHGANGTFCAGADLVAVSKSAFEDKPHFGPVEGRNIAPMGPSRMQISKPVICAVSGYAVAGGLELSLLADIRVVEEDAVFGVFCRRFGVPLIDGGTVRLQAIVGLGRAMDMILTGRPVGAQEALQMGLANRVVPKGKAVEEAMKIAEQLLNFPQLCMNRDRQSTYHAAYEAKSLEDALKYEFKHGEEVVGKESLEGAKRFADGAGRGGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0W5</accession>
    <name>A0A9W8Y0W5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LYR motif-containing protein Cup1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009316</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0W5"/>
    <dbReference type="OrthoDB" id="5521299at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20273">
        <property type="entry name" value="Complex1_LYR_unchar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046896">
        <property type="entry name" value="Cup1-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20263">
        <property type="entry name" value="LYRM2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LYR motif-containing protein Cup1-like N-terminal" evidence="1">
        <location>
            <begin position="17"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="437" mass="50778" checksum="31E5621EE8B1753C" modified="2023-11-08" version="1">MPVSLQQSASNLKSLHLLRALLREATYLPDATARTYFRQYVVNRYKAYQPKQNATASFDVQAVEKYRHRSFKRRQIAIINDRTRPLLRKGQKGLNFLRRANQGEIPCLQKILYFAYGRMGRRKYALLDSLLKPDLTVDGHAEPLPGSEELAPLQKLYYSNKRYLQYFDAPTPSSKTNYTIRISDRYSRLREVIKSQYQKGVKIRRELKSSALVTPINNVWERPMPIKRARNNVRRWYAATLSALLPPLPNDEWDNIRAMILKEKHISFAKRRKPVLEPEPADEERFAIAIEEGLALYTPSRANKPAGLHRPHTINVKFMRRLYTRLLMLCCKLDYDVEHKKWRATWGDSMKGISPTIYTMPTDGTLFAGVDAAGRVPIPPKKSDNDVETDSVKKTRLHKEAYVRFPFFADYLPKTHPVRKDLDRWKKKRAAAASESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1C3</accession>
    <name>A0A9W8Y1C3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009704</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1C3"/>
    <dbReference type="OrthoDB" id="1888931at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF115">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE FABG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4363411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="290" mass="30913" checksum="37E33765791A2E18" modified="2023-11-08" version="1">MPGSVASSVSSIIKPQPVLSCGESCDCCGCDESCCSQTQNNPDRDAAGLGQQILAAFALSGAHGAIVDLKQESADKSAETLLEEAKKGGLPKPKIHGYDCDTSSEEGVKSTWDKIIKDFGTVDILVTNAGITGGAPAEDYPYKDFKGMIDVNLTGTFLFSRTAGKWWIENKVDGRILIVSSMSGSIVNRPQKQSAYNASKAASAHLMKSLASEWAPHGIRVNALSPGYIQTEANAGEEMEKLSKQWVKDIPLARIAKPEEFRGAAVWLVSDASSYVTGSEIIVDGGYTIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1E4</accession>
    <name>A0A9W8Y1E4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cupin type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009428</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1E4"/>
    <dbReference type="OrthoDB" id="2446447at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02219">
        <property type="entry name" value="cupin_YjlB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047121">
        <property type="entry name" value="YjiB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36448">
        <property type="entry name" value="BLR7373 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36448:SF3">
        <property type="entry name" value="CUPIN TYPE-2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4363974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="27176" checksum="6BDCB11E19E0C471" modified="2023-11-08" version="1">MVVKVKTYKLPPTSLIPNSPYPLLHYPGLLRSEVSSPDFSTTDLFDLFARNGWQSQWIARYGPDIQSHYHSTTHECMAVISGQGATIRFGVADSKDWDHGKFPLGDRADGEEGGLELQAAMGDVFIIPAGVSHKTFKPRPGTQSLTFHQPEDIKEGKAKHVTPEKEKARREFFGNVVVEGEFMMMGAYPYGGVWDFAVGGEYKGHEEDVWHVPRPEKDPVLGLSEEGLIGLWAGNQEIAKQEVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1G5</accession>
    <name>A0A9W8Y1G5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Carboxypeptidase Y-deficient</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PEP7</name>
        <name evidence="8" type="ORF">N0V83_009220</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1G5"/>
    <dbReference type="OrthoDB" id="166134at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15761">
        <property type="entry name" value="FYVE1_Vac1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15737">
        <property type="entry name" value="FYVE2_Vac1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.20">
        <property type="entry name" value="Rabenosyn, Rab binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052727">
        <property type="entry name" value="Rab4/Rab5_effector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021565">
        <property type="entry name" value="Rbsn_Rab-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036531">
        <property type="entry name" value="Rbsn_Rab-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13510">
        <property type="entry name" value="FYVE-FINGER-CONTAINING RAB5 EFFECTOR PROTEIN RABENOSYN-5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13510:SF44">
        <property type="entry name" value="RABENOSYN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11464">
        <property type="entry name" value="Rbsn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140125">
        <property type="entry name" value="Rabenosyn-5 Rab-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
            <begin position="271"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="212"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="498"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4364624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="578" mass="65718" checksum="9942A195E086A40F" modified="2023-11-08" version="1">MVLPPNISQNRLALISIRHLDDNHQNLVEEEQDEVKNWFEAQMQKAKKFQPLAVLNQKLKGLDVFESNDAPTPPPMSHSHSNSATSAAAYAVPEPAPVRRDPEEEVTRSHWQRLGYRDACSDPICARPITASQMALGGSQLAVNCRQCGRLFCEEHTMYQMKLSRQAKHDPVRGIWCRVCETCYKSRDGYNDHHGFERNHFDEFTKFRRKRIDREQMEVSRLEKRLTKLTQLLANPPPLEETQTSGSWFSLSGAKNQRKALEQSIITWEDDAKVTNCPFCQQDFSNYTFRRHHCRMCGRVVCGDPKTECSTEIGLNVAAGKNRSEKGIGQMSVDVRMCKDCQHTLFARGDFQRELAEKPKDQRSYENLAQFERGIRLLLPRFQKLLQALQDPDKPPTSQQLSDATKVRKRLMEGFAQFDTAAKRIRDLPTDSPTQQRLQKAVYQQAYSFLSLHMLPLRSLPKILKHAAPQGRSNGAGALAAIKYNDRSAGSVVSSSEVSALESEEKELRERLIILEEQKFMVSEMVANATKHRRFDEVSSLAQNLEDLNKEIDQVNGQLGQLDFASAYQRDVASPAPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1H5</accession>
    <name>A0A9W8Y1H5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Suppressor of tub2 mutation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">STU1</name>
        <name evidence="10" type="ORF">N0V83_009235</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Microtubule binding protein that promotes the stabilization of dynamic microtubules. Required for mitotic spindle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLASP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1H5"/>
    <dbReference type="OrthoDB" id="46159at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060172">
        <property type="term" value="P:astral microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF9">
        <property type="entry name" value="CLIP-ASSOCIATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TOG" evidence="9">
        <location>
            <begin position="1"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="227"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="513"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="559"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="790"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="884"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1089"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="519"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="573"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="593"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="614"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="666"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="713"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4364639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1224" mass="133187" checksum="3B8CD8538A8D4408" modified="2023-11-08" version="1">MEEQTATLLAALKKPSTDVNKRLELFSNLKSGIKHNRVPESCQASIFECIRIAITASTSAALVSTGFSTLSHFIKRLQLQKETQAITSQSSALCSILQDKLGDARESHRSAASQILADLHYLCPTEVDTLVHEAIKGSNARAKETSMTWVVKMNKTEGLPFKSYSNQLVANLEDADPGVRDTAKRAVVELFGHAPEHAKVNLKKQLASLNVRKATVTFITSHIDDAAVSKDTEMPPPQPPQSQPRAERPLPARRAETLQPDVGFADSFASEIAPPTETVVMDPIHIYTQRELEDVFRDMAPHFEGRETEQNWLARDKNCTRLRRILKGNAPTEFHGAFTAGIKSLLDGILKVANTLRTTMSTNGSQLVQELAKTLGSAIDPWVEILLQSFIKMCAATKNIAAQNGNVTVDTILQNVSYSSRWLQHVSMASQDKNVQPRTHSATWVKTLIRKHTSHIEHSGGLDTLDKLIRRGVTDANPKVREAYRSAYWTFALVWPQRAETMFDTLDKREKTALEKDPNNPNASLASSQNSTASFSKSVGGGAARNALKEKIAEQRRAKLAASKGIPDRPNSAAATYSPAKSLSAKSLGGRITSTASTASTASAGPARPPSAMSGDSTKSALKNSTGTGSLMSGTVRRPIRPRPELTRPATADPYAIRRAGKVTPSMTPEKTPATTTAKKSVAPKSTARPRAQTQHSPSVSPVRSKSRIGQPISHQKSASTSSRQGSPVASPPKDEDLTMVKPWVRSQSQHDPGTIPFRQRNGMDRSALDNDTIDLGDEDNFTMVIPNLARPTAQSTQRTPPKPAPSASRLAVPSPRASALTSPKSIGDITSLRASTRSPRLRSPDRPSTRGTDAQEEVQIFEDPFVGDEPAAVEMEMEKPVLEELPLNERNNERRQSTQSTTSDTMMGNAGEERPRGHHKTTSTGSVMHAESYETNNAEVLKNRQLLASGIKKIEGRTVESHMFRRMQDMVKSNQEIWGPNDENFGRLLLACLDFLEAPIQELKISPMKGVNLKVQALATIRAMLSLYRRETAKYFSRVLCTILQTKAQYDNTSHIAVDLEATADEIIKYGQTSDCLNAVLSLVEATTPSVTPTSSPNSKSSSSTSPTPLSYQGGSSTASASRTTTMALITLASLIEISSAKNVTLTPEQTARLGKLAVRCMDDQDADVRKADIELCVALHERIAGLSGAEKEDGGGFWKAVAGAREQHLNLLTYYLAKRGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1M1</accession>
    <name>A0A9W8Y1M1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1M1"/>
    <dbReference type="OrthoDB" id="3799339at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="275" mass="33508" checksum="025CC21AC2FB6800" modified="2023-11-08" version="1">MGQQPEPTYHFENYENGKPKWAPDVDNQRDYHDYRDSRRDQRRPRRDRDAERYGNDAPPPPYPAAAPPPPREARPPRPRPQRAYSSSDSDSDAPPPPRRERRRDDRRSRREDTYDSYDEHYPPRRPREDGRKARDGYKSEGYKSDNRHTKPRRDVYDDDRARRDRPRDDRDRRGTSRDDRDRRDRHRDDRYDGLFKEPRRDARYDDRDRDRRGRYDDDDRYGGDRRKSDKRAVDKDKPVWQREAMGLFKDYAMPIIKKEGAKYMQKQMSGFMTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1U2</accession>
    <name>A0A9W8Y1U2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1U2"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="83" mass="9065" checksum="6973EE8B0B47951D" modified="2023-11-08" version="1">MFNRSSGDPSIQNAKQSLMQAEAAEREADRALYAAKNAVRDARAHVGRLEKEAAEEARLAKIKQGEAKALGKKGRALGRHDHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2F6</accession>
    <name>A0A9W8Y2F6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">5-oxoprolinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_007790</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxoprolinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3643at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049517">
        <property type="entry name" value="ACX-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF26">
        <property type="entry name" value="5-OXOPROLINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19278">
        <property type="entry name" value="Hydant_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="4">
        <location>
            <begin position="13"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="2">
        <location>
            <begin position="247"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Acetophenone carboxylase-like C-terminal" evidence="5">
        <location>
            <begin position="554"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase B/oxoprolinase" evidence="3">
        <location>
            <begin position="748"/>
            <end position="1280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4366155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1320" mass="141887" checksum="5ADC7B525A2BD1C6" modified="2023-11-08" version="1">MVAPIIPANKRVKIAIDRGGTFTDIWARVENQPDIVLKLLSVDPSNYDDAPTEGIRRVLSIVRGEEVPRGTPLPKENLEYVRMGTTVATNALLERKGAKHALLVTKGFRDLLDIGYQSRPRLFDLNIIKAEVLYSEVQEVDERITIEGFDEDIFGVHRATGEVSGSLIKGTSGDMIRILTPLDEQAARTTLKDLRSRGTETVAVCLAHSYIYPQHEARVGQIAFEEGFSHVSLSSAVAANMIKMVPRGSSASADAYLTPEIKKYLKGFLKGFEGGHLDDVKTDFMQSDGGLVGHKNFSGLKGILSGPAGGVVGFSRTSYVPETHTPIVGLDMGGTSTDVSRFGGVFEHVFETTTAGVTIQSPQLDINTVAAGGGSILAWRNGLLTVGPESASSHPGPACYRKGGPLAVTDANLFLGRLVPDFFPKIFGPNENEALDTEAVKSKFIELTKTINADTGKSMSPEEVAYGFLDVANEAMCRPIRSLTEAKGYEIGKHNLAVFGGAGGQHACEIASKLGISTAIIHKYSSILSAYGMALANVVQEAQEPVNVTYTGQSTEELDARLNDLRSKVKQALLNENIRESDIHYEDYLNMRYQGTETAMMILRPAGGSFKEAFLEQHQREFNFIFPDNRPILVDDVRVRGIGGSGNVNADADKLQHDLQHTAFAPVKATAVEREIKTYFAGVGVVATPVYLLEHLLPGSVVAGPAMILDKTQSILVVPGAQAKVLSVHVVIETPKFGNIQTSSTTVDPIQLSVFAHRFMSIAEQMGRILQKTSMSLNIKERLDFSCAIFGPTGGLVANAPHVPVHLGSMSYAVQYQHELLKGKLQPGDVLVSNHPEAGGAHLPDVTVITPVFSQGNEIAFYVASRGHHTDIGGLGGTSMPPNSTELWQEGAAIKSFRLIHNGKFDEEGIKRILTAPGDYPGCTPSRRLDDNLSDLKAQVAANNKGMMLIQGLMEEYGQDSVQLYMQAIQENAEIAVQAFLRQTLEKKGASFSGVDQMDNGSEMRLAVSIDPSGTATFDFTGTAPEMYSNMNAPPSITYSAIIYTLRLLIGIDIPLNQGCLSPISVILPKGCFLNPSSGAAVCAGNTQTSQRVCDIILKTFGAAAASNGCMNCLGFFGEVKNTDGTEHSYAFGETICGGSGATSVAHGASAVHTHMTNTRITDPETLERRYPVILREFAIRKGSGGKGRFSGGDGAIRDIECRLPIRFSVISERRAVAPYGMQDGDCGAKGANYWVKRVEKEDGSGFEERWVNMGPKNMVSMKEGDRCVIFTPGGGGWGRASEKGPVVNGVNGVKPAIKQQYPRAAGSVATRISAQGEAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2T7</accession>
    <name>A0A9W8Y2T7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ATP synthase subunit G atp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ATP20</name>
        <name evidence="11" type="ORF">N0V83_008001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase g subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2T7"/>
    <dbReference type="OrthoDB" id="437at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006808">
        <property type="entry name" value="ATP_synth_F0_gsu_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12386">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04718">
        <property type="entry name" value="ATP-synt_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4366365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="207" mass="21292" checksum="3691BC25E96FDD5C" modified="2023-11-08" version="1">MSLAASRAAVRQSTFAIRRAGLRNASTTSEAAGAVKGKAAEASSKASEGLTRVTSSASSAASSVGSAASNAASATTGRVGGIVGRVQGLIPQVTYYSKVALELGKLIAHQRSMAPPSLQTMQGYLQPAINAAKNPSSLLNRATSEANSAAQKVQQDPANILAQVRNVSRAQWLSAGVVAAEVIGFFSVGEIIGRFKLVGYRAKEQHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2W5</accession>
    <name>A0A9W8Y2W5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA ligase</fullName>
            <ecNumber evidence="7">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_008330</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2W5"/>
    <dbReference type="OrthoDB" id="206088at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07900">
        <property type="entry name" value="Adenylation_DNA_ligase_I_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07969">
        <property type="entry name" value="OBF_DNA_ligase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3260.10:FF:000004">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000062">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000018">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF9">
        <property type="entry name" value="DNA LIGASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="10">
        <location>
            <begin position="539"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="26"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="631"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="58"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="643"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4365710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="870" mass="96574" checksum="9744BF0EDB73EF74" modified="2023-11-08" version="1">MTPSKRRKKNDGSGQPVRSLDYFFAKQTAKATSTQTTTNTRDGVARVEQDTANLTDEELARKLQEQWDKEDQHHGQGGGPASSDELYEEPKKPAVNCSSNGDIEGVKIPESVVLAKSPKATASKQNNTLSLQSTASEEDTITANIPFDQSPLEFDPSTYIPDLQKHWVTDGGQATYALLTRCFVLVNSTSSRIKIVDTLVNLLRTIIEGDPSSLLPAVWLATNAISPPYIDLELGLGGSAISKALKKVCGLSDAGLRTLNHKYGDAGDVAFEAKKKQSFTLRKPKPLTIKSVFESLVRIANSKGQGSVENKQRIVERLVQDARGAEESRYVVRTLVQHLRIGAVKTTMLIALSRAFMLSRPPSADFEIREQKAMAKLKKEELAEIYSRNEEIVKACFARRPNYNDLIPALLEIGVCDELLLRCGLALHIPLRPMLGSITRDMGEMFTKLQGRDFACEYKYDGQRAQVHCDEKGKVTIFSRHLEVMTDKYPDLVALVPKIRGEGVASFILEGEVVAVDRESGDLKTFQTLANRARKDVVIGAVTIDVCLFAFDLMYLNGEELLDRPFRERRSLLKSLFVEIPHHFTWVNCLDATSADVEAVQNFFKSALDIKCEGIMVKVLDNLPNPDLVGEVTEDAPSLPPTPKKKKPAKSKTKSMVTSDADKDEPKSTGRRKALLSTYEPDKRLDSWLKVKKDYSTTSETLDLIPIGAFHGSGRKAAWWSPILLAIRNAETGQLEAVTKCMSGFTDAFYKANRAKYDPDDPDNANTLGGKPSYVEYNGGAGWPEVWFEPQEVWEVAFADLTLSPTYTAAIGLVSEERGLSTRFPRFLKVREDKGIDEATEAAELATLYRKQEAKVVKEDVKEVDEMDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3D4</accession>
    <name>A0A9W8Y3D4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_008008</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3D4"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015793">
        <property type="term" value="P:glycerol transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000061">
        <property type="entry name" value="MFS sugar transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF69">
        <property type="entry name" value="SUGAR TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="284"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="424"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="447"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="27"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="509"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="526"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4366372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="546" mass="60152" checksum="12DF1EB533B8A7AF" modified="2023-11-08" version="1">MAPRIKDNDSGVPTYWGTSGRMLQVLITIVATTDFLLFGYDQGVMSGIISAPAFQAAFPEVQDDSTYEGFVVSIYAVGCFLGACFIFKFGDKLGRRRAIFLGASVMIIGVIIQIATVPVSGGATAQFIIGRCITGIGNGINTSTIPTYQAECSKAKNRGKVICIEGSMVAIGTLIAYWIDYGCLYGPDAFTWRFPIAFQCVFALIVIIMMIALPESPRWLLTHHREEESATVLAGLNGLQRDDPEVLVQMQIIKDAVRHSGGGGKAPTKSLLTNGKSQHFRRTILGASSQMMQQLSGCNAVIYYFPILCQDVLGTDHNLALLLGGVNMVVYAIFATTSWFLIERVGRRKLFLIGTVGQCLSMVLTFACLIPGTESTAKGAAVGLFTYIAFFGATWLPLPWLYPAEINPLKTRQKANAFSTINNWLWNFFIVMITPVLITQISWGTYLLFAALNACFLPIIYFFYPETSGRSLEEIDLIFAKGFVEKKSYVHAAKELPQMDESAIAQANRDYDVHSSSDEEDQESTDEIKEKRRQEEDLMPQRGAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3J3</accession>
    <name>A0A9W8Y3J3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_007383</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12 13">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3J3"/>
    <dbReference type="OrthoDB" id="6287070at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF722">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="205"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="649"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="821"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="411"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="673"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="691"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="713"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="732"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="766"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="845"/>
            <end position="879"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4366855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="879" mass="99057" checksum="3E3D1783457A7E1B" modified="2023-11-08" version="1">MNGVSRFLSRRDKHHEKRAAKNAQHKVRSNSFTSIDSTTPLLFSSSGIEPRALKDRQGRQSSSLEALYCKLPSALRTPVEDSVVFLRKHTQSRAILTAITIQTSTKNSSDYLILQSRPSQVSSDLYTIFTNEDLRTAADKDVEKKVKMLLMRLQGAGISRMGEEQAHHALSWYPGDLDKAYDVLVLANESIEGELKDYNANVTMVGAINRNMVTCYLDALLFAMFARLDSFEAMLYDNFDDEPRKKLAAVLRLWVNLLRTGQLIKVELTKHLQDSLAKCGWEDAAQVCQQDASEAFTFITGALELPLLTLKMDIYHTGREDKEDDHKFVNERLLEVAIPEQDGDHVITLEDCLETYFNNRIEVKRYLQRQNTIASIKSREEGSNDKDPEKSDIIHVEALELSGSESPIVATPTSLGPSTPVTPVRPLEGRRRADSIFSQRYTRKSPEAIKFDEKKHLQDMLDNSSGGRPRSASLLRKEILMPAWQFFSLIPWYTDNAPKTDAQVAAHFSVKRPVLGICLKRYTMLPNGEPKRLDTYIDIPLDIGLPHFISDDRMKEEGPLFGNFKLVLQSVVCHRGVSVDSGHYIALVRDNAHELPRTIRSDDEDSQGTWLRFDDLSTPRVTEVDIKRALREESPYLLFYQVQPINEELASRGDPPPYAEPDSGFPSADTSRETLSSIPSTITTDTETVGDWEKLASTDMHHESSHSDERIGRNSTSSNRRSSVTFEDIDGSTYSVSRGRTQPPTPDEQKPGFLSVSRRGSRTWLPGNNKSRPSSQSGEGRLSLTLSRLTGRSSKDKLQITEGIATDDPVIVINEVQSAEVVQRAHSPVKERKETGLSRSKSKREKKDRLRSKSRDAGEGLEKGKYKEKNRPDRECILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3V9</accession>
    <name>A0A9W8Y3V9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="14">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">RPB2</name>
        <name evidence="23" type="ORF">N0V83_007811</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="14"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3V9"/>
    <dbReference type="OrthoDB" id="10248617at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.150:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1070.20:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1110.10:FF:000013">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1800.10:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007175">
        <property type="entry name" value="PRK09606.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14 23" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 14 23" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="19">
        <location>
            <begin position="40"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="18">
        <location>
            <begin position="292"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="20">
        <location>
            <begin position="502"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="21">
        <location>
            <begin position="601"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="22">
        <location>
            <begin position="708"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="16">
        <location>
            <begin position="788"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="17">
        <location>
            <begin position="1162"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="746"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF04567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ4366176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1263" mass="143076" checksum="4820F62F4296E58C" modified="2023-11-08" version="1">MADYYDDHEMEDEYEDEENDQMISPEDCWAVISSFFDTKGLVSQQLDSYDEFTRNTLQDIVKENGSVILEQNTPYNPDEEDEPIIKRRYEIKFGRVSLARPTHTEGDGTTVQLYPHEARLRNLTYSGAMMADIENRTMVARETHAPADGEDEEVEGGYTGGPTQIRWEREDAPVDDVTAAKVFIGKLPVMLRSELCHLRDRSDADLFGLNECPYDQGGYFVINGSEKVLIAQERSAANIVQVFRKKQGPIPWTAEIRSAVEKGTRLISSFNIKWAENSLAGAGKRVPGPFAYATLPYIKADVPMAIVFRALGIVSDEEILSHIVYDRTDTQMLELLKPSIEEGSVVQDRETALDFIARRGANSGTRDRRLKFARDIMQREFLPHISQKEGQDTRKAYFFGYMIHRLLQCVLGRRDEDDRDHFGKKRLDLAGPLIANLFRILFLKLTKDVYKYLQRCVENNQEFNVQMAVKAAIITNGLKYSLATGNWGDQKKAASAKAGVSQVLNRYTYASTLSHLRRTNTPVGRDGKLAKPRQLHNSHWGLVCPAETPEGQACGLVKNLSLMCYVSVGSDASPIIDFMTQRNMQLLEEYDQNQNPDATKVFVNGVWVGVHSNAHQLVSIVQELRRNGTLSYEMSLIRDIRDREFKIFTDAGRVMRPLFVVENDIRKPNRNQLIFTKAISTKLLMEQQSHDQRNGWSEQEIEAATYGWKGLIQDGVIEYLDAEEEETAMITMSPEDLDEWRDMKQGIPPNERANQGKDRLRRIKPKPDPRIHSYTHCEIHPAMILGICASIIPFPDHNQSPRNTYQSAMGKQAMGVAITNYALRMETMMNVLYYPQKPLATTRSMEYLKFRELPAGQNAIVAIACYSGYNQEDSVIMNQSSIDRGLFRSLFYRAYTEQEKRIGVNVLEQFEKPVRFDTLRLKGGTYDKLDDDGVVAPGVRVSGDDIIIGKTAPIASDAQELGQKTSTHTKRDVSTPLRSTENGIVDQVLFTTNTEGLRFVKVRTRTTKVPQIGDKFASRHGQKGTIGITYRQEDMPFSREGLTPDLIINPHAIPSRMTIAHLVECLLSKVGAINGQEGDATPFTEITVDQISDLLEKAGYQKRGFEIMYNGHTGKKMRTQIFLGPTYYQRLRHMVDDKIHARARGPLQILTRQPVEGRARDGGLRFGEMERDCMIAHGAAAFLKERLFTVSDAYTVHVCDICGLMSPIAMLTKNQYECRPCHNKTRISQIHIPYAAKLLFQELLAMNIATRMFTTRSGLSVRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y522</accession>
    <name>A0A9W8Y522_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Threonine synthase</fullName>
            <ecNumber evidence="5">4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">THR4</name>
        <name evidence="15" type="ORF">N0V83_006937</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-homoserine + H2O = L-threonine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10840"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.2.3.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:10841"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the threonine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y522"/>
    <dbReference type="OrthoDB" id="5203861at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01560">
        <property type="entry name" value="Thr-synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000046">
        <property type="entry name" value="THR4p Threonine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1380.10:FF:000003">
        <property type="entry name" value="THR4p Threonine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1380.10">
        <property type="entry name" value="Threonine synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029144">
        <property type="entry name" value="Thr_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037158">
        <property type="entry name" value="Thr_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051166">
        <property type="entry name" value="Threonine_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42690">
        <property type="entry name" value="THREONINE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42690:SF1">
        <property type="entry name" value="THREONINE SYNTHASE-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24857">
        <property type="entry name" value="THR4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14821">
        <property type="entry name" value="Thr_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Threonine synthase N-terminal" evidence="14">
        <location>
            <begin position="9"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="13">
        <location>
            <begin position="97"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR604450-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF14821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4367356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="547" mass="60734" checksum="98212FB62F8E4084" modified="2023-11-08" version="1">MSSHTASQRYLSTRGGSYDLSFEQVVLKGLASDGGLFIPEEIPTLPDHWASSWKDLSFEDLAFEIFSLYISPSEIPPADLKNIIHRSYSTFRAKDVTPTVTLDKEKNIHLLELFHGPTFAFKDVALQFLGNLFEYFLVRRNQGKTGRDREHLTVVGATSGDTGSAAIYGLRGKKDVSVFIMHPHGKVSAVQEAQMTTVMDANVHNLAVDGTFDDCQDFVKALFADPEINATHRLAAVNSINWARILAQITYYFYSYFNLIKHESFLPASTARFVVPTGNFGDILAGFFAKRMGLPAEKLVIATNENDILHRFWESGRYEKHPVHGKQAEGGLVEDGVKAHESGVKETLSPAMDILVSSNFERLLWFLAYDVYSTNSDAVSQRRSQAGDHVRGWLNDLKSKGGFAVDQQILEAAQSDFASYRVSDEETIDTIKYIFNAPSSRSYVLDPHSAIGIAAALRSAEVAKPPSVHHIALSTAHPAKFANAVELALPEQKEYFQEHVLPIEFKGLEDKPRRVSHVQKSDGWQGVRKVVVAEVEAELQAAEKAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5H7</accession>
    <name>A0A9W8Y5H7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">proline--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Prolyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_006672</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Pro) + L-proline + ATP = L-prolyl-tRNA(Pro) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5H7"/>
    <dbReference type="OrthoDB" id="1350766at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00862">
        <property type="entry name" value="ProRS_anticodon_zinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00778">
        <property type="entry name" value="ProRS_core_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.30:FF:000001">
        <property type="entry name" value="Bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000007">
        <property type="entry name" value="Bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000005">
        <property type="entry name" value="bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.30">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01571">
        <property type="entry name" value="Pro_tRNA_synth_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004499">
        <property type="entry name" value="Pro-tRNA-ligase_IIa_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016061">
        <property type="entry name" value="Pro-tRNA_ligase_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017449">
        <property type="entry name" value="Pro-tRNA_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033721">
        <property type="entry name" value="ProRS_core_arch_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00408">
        <property type="entry name" value="proS_fam_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382:SF2">
        <property type="entry name" value="BIFUNCTIONAL GLUTAMATE_PROLINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382">
        <property type="entry name" value="PROLYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09180">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SMART" id="SM00946">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64586">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="11">
        <location>
            <begin position="88"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4368316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="562" mass="62982" checksum="DDA6C04ED72A58A1" modified="2023-11-08" version="1">MAEASAADGKMKDLSISETKKNKKDGNPKTAAKKPQQQKKKIEGAALIGIDVAKEDDLGEWYQQVITKGQMISYYDVAGCYILEPSSYAIWESIKAWFDSQIKTLKVRNAYFPIFISADNLEREKEHVEGFAAEVAWVTKGGKSDLEKPVAVRPTSETAMYPYFARKIQSHRDLPLKLNQWNNVVRWEFKHPMPFIRSREFLWQEGHTAHLTQKEASEEVLQILDWYADVYQNLLAVPVVKGTKTVNEKFPGADYTTTIEGFIPATGRGIQAATSHCLGQHFSKMFNITVEDPTAKEAGKSEHVHVWQNSWGLTTRSIGVMILTHGDNRGLVIPPRVAEIQVVVIPVGVTAKMSAEAKENLYSKVKKISDDLIEAGVRVETDYREGYSPGWKFNDWELKGIPLRIEFGPKDAEKGVVTTSRRDIDDKDSARGTANVDDVKTEIPKLLEQIQTDMFNRADKAYREHRIQLTSWDDFVPTLNGKNVVLVPHCEGEKCEDEVKAKSARTALGDGVAEDKQAPAMGAKSLCIPFEQPEGIEAGKTKCINPDCTTPAKQWVLFGRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5I0</accession>
    <name>A0A9W8Y5I0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Scytalone dehydratase</fullName>
            <ecNumber evidence="7">4.2.1.94</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SCD1</name>
        <name evidence="7" type="ORF">N0V83_008094</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the scytalone dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.94" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5I0"/>
    <dbReference type="OrthoDB" id="5281072at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030411">
        <property type="term" value="F:scytalone dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006582">
        <property type="term" value="P:melanin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00531">
        <property type="entry name" value="NTF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004235">
        <property type="entry name" value="Scytalone_dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049884">
        <property type="entry name" value="Scytalone_dh"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02982">
        <property type="entry name" value="Scytalone_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF024851">
        <property type="entry name" value="SCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Scytalone dehydratase-like" evidence="6">
        <location>
            <begin position="9"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR024851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR024851-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR024851-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4366458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="184" mass="21224" checksum="B668F268FCB32B0C" modified="2023-11-08" version="1">MSQQKLKPTFDDVMGCQSACYEWADSYDSKDWDRLRKCIAPTLKIDYRSFLDKIWEAMPADEFVAMASDPAVLGNPLLKTQHFIGGTRWEKTAEDEMTGYHQLRVPHQRYTDESRTKVAVKGHAHSFNTHWYKKVDGEWKFAGLNPDIRWFEYDFDKVFADGRDSFGEAQEAQLASGVPTKVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5N1</accession>
    <name>A0A9W8Y5N1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007319</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5N1"/>
    <dbReference type="OrthoDB" id="3795586at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021514">
        <property type="entry name" value="DUF3176"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35394">
        <property type="entry name" value="DUF3176 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35394:SF5">
        <property type="entry name" value="DUF3176 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11374">
        <property type="entry name" value="DUF3176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="533"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="656" mass="72467" checksum="D66FA4863475C244" modified="2023-11-08" version="1">MQPGDETPMLLTDDTTSQPTTQQTIPLVLTKWLPEIISVLVALAVLLEIVILLAVYDGHPNPLWSGGITLNAIISFASMVFRTSLMVPVASCLSQLSWVWLAQGRRQLFDVVRFDQASRGPYGSLKLLFWHPVQSYTFIGACVTILSLVIGPFPQQSMVFYSDLVIDSSTISYASYAFDCNASVGFLSPSHEGPALRTPFNMKSAIYNGIFSSVTTSPPVPPFSCPSGNCSWDPFPTLALNVQCRDAPEQYRLNCSEADYHGETFPERCMIVAAEEPAKKTHLIMFPTEVGSTTMHFAISYVGTVSDLNASLWTMPIGGRVDFEWVRATDLLIARNNESDSWQSYVARNSTIESRQCIFSTSLQSVTAYVVNGAYDEFITNDLVNATANDAKQVVNTTYTYAYTGYDGISWSMTLPEPQQEALLTDICIPFQDLKAARGSVLSFNRYANVSMSSDDELIGADIMKTLYYVPNLTETAHAIARHMTVALRSVQTSIDLQDDNLNNGDLPNDYIAPKHRVPGLVYINKIHVLVRWGWLALPAFLAALVCALLIATILISEKENVGVWKDSPLPLLLFSRWEGERKSDIGHAQTEKDIRRAAEGIRVELGNDIDESGSIQDRIEVKKLGAKSNKERAAESISGFSFSELLPYTSKSTNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6Y2</accession>
    <name>A0A9W8Y6Y2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_005868</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6Y2"/>
    <dbReference type="OrthoDB" id="5204190at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00105">
        <property type="entry name" value="KH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="208"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="309"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="421"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="15"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="107"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="279"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="384"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="491"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="152"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="390"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="491"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="505"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="520"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4368786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="575" mass="61375" checksum="B670E2EBAEFD9224" modified="2023-11-08" version="1">MAAPPDLNAILAALAAGRGPSTTPSQTPQQPPPAQHALPHGMPPGYPGAMPAASPPAIPGFNFPQPTNSGSLDLSAIKPVSSGSVSIQDALAKARGFAAQKGLAFDARPGIPEPWSGYGDPSQTYAVTAHQEDPRLSGRPYRRSRSRSRSPQRRDGARDGYNPYRDERRDERRGGGYGRDRSRSPLRRDTFSPSQAQYGGHSRSPPPSDNSEVILIDSSLVGLVIGRQGESLRRIEQESQTRIQFINGPEAGPQRQCRITGNPGARISAKREINRIIEENGGNPARETGRNARPNAKAVGQQQPALRDGEQSSQIMVPDRTVGLIIGRGGETIRDLQERSGCHVNIVGENKSVNGLRPVNLIGSPAAAAHARELIMEIVDSDTKQMDASNNQQPQAHQQQHNNKRDNFDSYSGGGGGAGAGKINDSMMVPSDAVGMIIGKGGETIKSMQSDTGCKINVSQASGADIEREIGLVGTRQAIEDAKRAIWEKVDQVKEKNNSRRRDNGNQNDNYSHQQAPSYGQNAPQAQPPAAAAPNVAGQAADPYAVYGGYQNYLAMWYASIAQQNQQGGQGPPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7G4</accession>
    <name>A0A9W8Y7G4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ethionine resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ERC1</name>
        <name evidence="8" type="ORF">N0V83_006241</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7G4"/>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="366"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="466"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4369157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="524" mass="57164" checksum="5B472C37176FDAF1" modified="2023-11-08" version="1">MDYEASKSRSWLPKFLRKSKAEEANGAPTERTSLLPPSDQEEWSTGESYELEEGSASSFHLILQEFWILLKGSLPVVLAYALQNSLQTISVLIVGRLSPEALATAAFSYMFAMATGWLVALGGTTAIDTLGSASFTGSKNRHDLGIVLQRSFIVLLLFYIPITILWFCSEPLFKALGQEDYIARDSAKFLSVLAPGGLGYIYFEALKKYLQAQEIMRPGTYVLLITSPLSAGLNYLFVYTFRMGLLGAPLATGISYWASFLLLLAYARFVDGWECWGGWNRKCLQNMWVFARLAFLGVIHVGTEWWAFEIVALVAGKLGTIPLAAQSVIMTTDQVMNTIPFGVGVATSSRVGNLLGARNAKGAARAANTAAVLSMILGALVLAVLMGVKDFYAKIFNDDIEVIKLTAEVMPYVALFQIADGLNGSCGGALRGMGRQHIGATVNIVSYYCGALPLGIWLAFHGWGLGGLWVGQCIALYLVGFGEWAIVAWSNWDYQVKKAFDRMDSDDRAELGVPQEVAPSAVYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7Y8</accession>
    <name>A0A9W8Y7Y8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004931</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7Y8"/>
    <dbReference type="OrthoDB" id="5387895at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="441" mass="49496" checksum="9F6492CF58DAE534" modified="2023-11-08" version="1">MSAEQFSKLSLGDAMVTTRAGAAKAARDSPKSPQDQDHDQPLPSIESPPVSPTPSLVISTNNLQYNVSAFGSDLRKRAKRGLKENEIRMKYCAVTDNEDSPDSTKYFYIDDDITHIYWVGDQILSTVSDTLSDQPLQLSADGSVIEDMKPAEILDEKSLASIANDLQWVVREDVVPEDDDEMRDAIISMLSVFEPQDALPGEFKCPESPLASERSKKYQEFADVFTQYATRDPGLYLQVRNIIDPNFQGHVFFDKIDTRVTRTFNALDEYITHGTTNVSPETPRFDVPSCAKELRALVQAIDEFYKQQMDDDRDTRDVAVRAAAALVTILDRVTDRNVNVYEHITWGMEASADPVENNLFVALIGSYDGVGPSFVLSALMSLPQEDVLRNHWETLQNIEQKLANPSTPLEYTTAFRKVVYETRKRAASEVRGGESKRTMQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8K0</accession>
    <name>A0A9W8Y8K0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Enolase-phosphatase E1</fullName>
            <ecNumber evidence="4">3.1.3.77</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">UTR4</name>
        <name evidence="4" type="ORF">N0V83_005738</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.77" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8K0"/>
    <dbReference type="OrthoDB" id="272500at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043874">
        <property type="term" value="F:acireductone synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023943">
        <property type="entry name" value="Enolase-ppase_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01691">
        <property type="entry name" value="enolase-ppase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20371">
        <property type="entry name" value="ENOLASE-PHOSPHATASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20371:SF1">
        <property type="entry name" value="ENOLASE-PHOSPHATASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4369974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="156" mass="17114" checksum="1B93898162A5C467" modified="2023-11-08" version="1">MPRQLTPIAGHLWTTGFTKGDLKTPLFEDVVPTLEAWKAAGKTLAIFSSGSVQAQLQFFSYVRDGQTTRDIKPLFSAHYDTVKAGSKLEQGSYTNICSELDQDTSKVTFLTDNVKEAEAATEAGVYTVVVDRPGNAPLSNESKQRYPVIQKLTDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8X5</accession>
    <name>A0A9W8Y8X5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8X5"/>
    <dbReference type="OrthoDB" id="3790651at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="445" mass="49887" checksum="643147461E4E930B" modified="2023-11-08" version="1">MLQGLAINTIAVPKPRWYPNYLDTWRLTAQLRNEMTVEHPKVLLQSIDWYNLLAVGQSNGQNDGYEPWDWALGLSAAYSFVGLSHVVSTVAAPEKNWQHIYRMQLDGDPLRRWTGLNTAFHQPDRRVETISMDDEQVIQVFEWLRKMGHKLTSSSTGWTGNLTLILPALNTICVSTVQPNATESFNITVPAGGATGEPLFSIHLGSVASRNFSGATCTSTFRQALYPVNFWIVNMQGADASFNGFGNDWDKTIVYEAITPADYDIVHSLAIQARDTLGRMEPMVHTATLLEHFLLISRMLQKTNTSIHSDAEGLSIVAGTLLQNILSVSNKYRSPLPSTRPFDPQEMVKSYPLRWQVYGSGPRLSWEWITIVILIVVVACFAFGIYQTLRFRMGPGPWVELGGMMMLAQTSPKLDDIGDKEKARKSTYWIHKEVTGETVLKSKAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y926</accession>
    <name>A0A9W8Y926_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y926"/>
    <dbReference type="OrthoDB" id="42889at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF137">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="643" mass="71279" checksum="5BB4E874F57A796B" modified="2023-11-08" version="1">MASKDFSTHPLANDLVAWFTHNGGSLSPDVQVVNSASRGFHMRAVRPLSSPVVATCPLKLTLSSLNLDPNEKEVLHVESPLRQCQGKVPNHILTYLLLFEERNKGKASPWHAYIACLPGPESMTTPLWFDDEDMTFLAGTSLAPAARERKEDMRRQFDEAVVVMQELGIALANEINFDSFLWAATIFTSRAFISTHILPNRETIPLLFPVVDILNHSVSAKVEWDFQPHQSFSLKCLEGETFAPGQELFNNYAPKQNDELLLGYGFCLEDNPIEQFALKLVFPPELQEDVQEMGLMHPDNVPFGMSSTFLDTDPNTEQHFLRAKGHPFGRYENWVPYLRGIPPFIVHFFFIQTLLARDIDVRDVNIRKPDIRITLQVLALLHSALSTRCLSLPPAISLSPKTDKQKYAKIYRDGQAAIIHSVLRELEAAGAQLRIPPPPPSGSQTPAPTSRRPVLLSTADFITALEAAFPTAAQQFRTGLPKHALTTSYDDDPPSLDAPPRTTSLLTSYIHALTTQHPLPALEDGIADAETYTFIDEHLGDFLHLPSLYAPDEDASPIDVLDDLGLTFVNQPADGGEKEVFVKGKTENLGARVLMWGMKVAEVELFGVWEEGRAKRCLLVAGSQGEDGGEGGGDEWLYGDVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y938</accession>
    <name>A0A9W8Y938_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Erv26 super protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SVP26_2</name>
        <name evidence="7" type="ORF">N0V83_005191</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SVP26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y938"/>
    <dbReference type="OrthoDB" id="28257at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097020">
        <property type="term" value="F:COPII receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007277">
        <property type="entry name" value="Svp26/Tex261"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13144:SF0">
        <property type="entry name" value="PROTEIN TEX261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13144">
        <property type="entry name" value="TEX261 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04148">
        <property type="entry name" value="Erv26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4370670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="249" mass="27054" checksum="6798F56C4EB4E546" modified="2023-11-08" version="1">MWLLPLLGYVGVLLGFAFLTLAIASGLYYLSELVEEHTVFAKKLLFRLIYGVVAIQVLLLVVDRFPIGLSALSIVSHVIYAQNLRRFPIVKLTDPLFLLSCALVIVNHYLWFRHFSAPPPSSAYSAYPYARDANIPSFTEIASFFGLCVWLVPFALFVSLSAGENVLPSMGSEYATGEGSSYITPGKAPDAFGSGPGVGVGDVMTGKRRARSGTNAGMAKAVVTGVREWVGETGEVMGFWKGERTRPTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y943</accession>
    <name>A0A9W8Y943_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DUF726-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_006212</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMCO4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y943"/>
    <dbReference type="OrthoDB" id="277931at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007941">
        <property type="entry name" value="DUF726"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920:SF22">
        <property type="entry name" value="DUF726 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_2G12860)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4 TMCO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05277">
        <property type="entry name" value="DUF726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="445"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="59"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="630"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="62"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="630"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="670"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4369128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="711" mass="77528" checksum="60DF9539F102A22F" modified="2023-11-08" version="1">MASWAPKLPSFGHAKSSEKDETSLTTLLTAEQCADLTFLIATITASMRQSLLDTFTAEEAPADSKIEKSKEDALKSAPSDLDKEDKVKKERQSREEDARKELAQVEVQELKKEMLRYFDGWRGKVIGRVGEIVNSREEAKEQAKHVEGKQVQQIVKKSEAGMTSIHSLDAKDADEEKAFRNIYPPVPNALTHLDEAKRALVLHSLVLILLSLEHYSAHSRILILHLTSSLNLELSVLKKDEETVAQGLLEAASQQLNADAETKKAAEASQTSRKWKVGLAGVAGAALIGVTGGLAAPLLAAGVGSVMGGLGLGATAAAGYLGTLAGSTVLVGGLFGAYGAKMTGKAMDAYAREVEDFAFIPIHRSQDPKHQDNESRRLRVAIAVSGWLREPEEVSKPWRYIGKGTEGFALRYELEALLKLGHSLETLVTSAAWGYAKKKIIEQTVFAALSAALWPLGLLKVATIVDNPFSVAKFRAEKAGEVLADALINKVQGERPVTLVGYSLGARLIFSCLQKLADRKAFGLVENVVLLGAPCPSDATDWRRMRAVVSGRVVNVFSKQDYILGFLYRTSSVQLGVAGLQPVVGVHGVQNVDVTDLVDGHLQYRFITGSVLRKIGFEDVDVAEVKRAEAELNKQKEEEEQERRKNEKQTDGEGEDKEKLEGEVQKKSEQSIFQLMQEKTQNLDIAGFLAGGRKDNHGHEGAQAQQKSEMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9D3</accession>
    <name>A0A9W8Y9D3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease H</fullName>
            <ecNumber evidence="4">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_005286</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase H family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9D3"/>
    <dbReference type="OrthoDB" id="3798049at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.970.10:FF:000002">
        <property type="entry name" value="Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.970.10">
        <property type="entry name" value="Ribonuclease H1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_bL9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011320">
        <property type="entry name" value="RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037056">
        <property type="entry name" value="RNase_H1_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38846">
        <property type="entry name" value="C3H1-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38846:SF1">
        <property type="entry name" value="C3H1-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01693">
        <property type="entry name" value="Cauli_VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="L9 N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribonuclease H1 N-terminal" evidence="12">
        <location>
            <begin position="54"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="290"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="395"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="321"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="367"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4370764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="457" mass="51600" checksum="02286F893D4E7F59" modified="2023-11-08" version="1">MSAPTWRADVPGASKLFSGLTRRDAFGHQRKGLQQSLSLYANQKSEDMEPRFWKYYAVAVGRTPGLYSHWDDAREQVMGFKGSRWEKFMDRSDAIQFMREYGFADAEIRVFRKTFKPSSTFEPNPTATFQDEFKAFAATQTWKQEEARKAKVDTIVGELIYHYLPEGIAADQEDEDGAVDLTDEQALKIYQGMCRSAGKPSHPRIDNCLLELKKAPYVNIIDFVNTFRTGQEVQPFARWIAFREYTLQDGHRIDIQYAKENEFLAPLLQDLRPGRRRAVCPIQVRRDFVARKAKKPQQQKHDMPPPEVKVSSSMIPEICLSPPASDFSPPASRSPTPDLDDDDPISALGDHADTTAHGPHIKLESPSPDPIVTPDPPSRFALAAVSKEEDDVSGFLLPEGLPLSSQLEERHLRDPPPSSQMEQRHRTPMASAGKRPCASMPANFTPSKRARRIDFAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9G8</accession>
    <name>A0A9W8Y9G8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Short-chain dehydrogenase/reductase 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Retinal short-chain dehydrogenase/reductase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">N0V83_004713</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the reduction of all-trans-retinal to all-trans-retinol in the presence of NADPH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9G8"/>
    <dbReference type="OrthoDB" id="5840532at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052650">
        <property type="term" value="F:all-trans-retinol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000131">
        <property type="entry name" value="Short-chain dehydrogenase/reductase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322">
        <property type="entry name" value="PKSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322:SF736">
        <property type="entry name" value="RETINOL DEHYDROGENASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Ketoreductase" evidence="14">
        <location>
            <begin position="101"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4371495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="367" mass="40016" checksum="C877E395B263314A" modified="2023-11-08" version="1">MSSAIKEVPVQLPKSFDRPDEPWHHYLTIDVIWYVLGYTLFHPFVSWVLVLCLRAQYTAYHAPEMRIAFAWAALMTAIGLFGLISDRIAFGSPREVDLSEEVIVITGGVEGLGGLLAETYGMRNANIAVLDTKKVDEDEAEEKGAVYYQCDVSDAKQVEAAAAKIIEDLGAPTILVNNAGLVHTKSILESTAEDVEQTFRVNTLSHFHTLRTFLPHMIAEGRGTVVTVSSVLGHLGAANLSAYTASKAALLALHHSLRAELAQIPEAKEIKTILVTPGQMTTRMFAGVKTPSNFLAPVVTPADVGKDIIRLIEKGESGEVALPLYSRYIHILGVLPVGIQYLVRRWSGIDSAIGKAGLTKKETSRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAA4</accession>
    <name>A0A9W8YAA4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_005727</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAA4"/>
    <dbReference type="OrthoDB" id="2157530at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF82">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4369963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="396" mass="45085" checksum="DA76799A2AAB974D" modified="2023-11-08" version="1">MVYIGRFRIPWSGFEAGARFFNDLFSEHPSVRLTIQDRDLVAKCFRQFAKIEGFSRLREYGPKTSLEARMYNSMLQKATDPRDHIYGILAISDIEQELLAPDYSLSVQQVFTKTTAALLAFYSPTFYCIYPLRCGLKDVPKRMAKTPDLPSWVPDLAACATSGMYETQESTYWAPTLLLQIPDIAPILRPFVERLEHPCIAKFSEDFKTMFAIGNALGTITWASVSDLFVGPGAARRLHNSYHTSVKKQGFTPALFLQVIHDYRRIFLQPSDKQYFIDFLESDSVPDDFDPDYTAIMENILGSASARTLFVTDNGKFGRSYNSGQDGIRAGDVLVGLFGNNFPFILRPIGSEKYEMINVAYVLDHQWGELHPAIDNSEYDSWSDLEKFGFREYEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAE7</accession>
    <name>A0A9W8YAE7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endoplasmic reticulum lectin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Protein OS-9 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">YOS9</name>
        <name evidence="11" type="ORF">N0V83_003818</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Lectin involved in the quality control of the secretory pathway. As a member of the endoplasmic reticulum-associated degradation lumenal (ERAD-L) surveillance system, targets misfolded endoplasmic reticulum lumenal glycoproteins for degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
            <orientation evidence="1 7">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the OS-9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAE7"/>
    <dbReference type="OrthoDB" id="448954at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045149">
        <property type="entry name" value="OS-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012913">
        <property type="entry name" value="OS9-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15414:SF0">
        <property type="entry name" value="ENDOPLASMIC RETICULUM LECTIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15414">
        <property type="entry name" value="OS-9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07915">
        <property type="entry name" value="PRKCSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 7" id="KW-0430">Lectin</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040935644" description="Endoplasmic reticulum lectin" evidence="9">
        <location>
            <begin position="17"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="10">
        <location>
            <begin position="159"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="60"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="216"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="480"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="502"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="524"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="536"/>
            <end position="546"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4372045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="554" mass="61045" checksum="FA04D3BA648290BA" modified="2023-11-08" version="1">MRNFWALPAVLRIALANQHAFSVFDDLLAFPQVWRATNEDRRYEIVWPDTFITEDGATSLLSRQTSPSSSSATPGSQETQELSKRDKPSSHVPLADDALEQTYEAVVLQGQRYLCSIPIIPDELPQNSTTTAEEAKAEEEKELMRASDRGFELLEGMRGNCIYYISGWWSYSFCYKDEVKQFHQLPPSRGVPIYPPVEDTSVHSFVLGKYPKEAKGKKEDARKTLGSEQGSKEAFDDEGNAKDEEKGLELPRLESKGSSRYMVQRLSGGTECDLTGRPRKIDVQFHCNPQSADRIAMIKETSTCSYLMIVDTPRLCHDVAFLPPQENLAHPITCQPVIPESQADAWTASKLEAGLREVESLILGEHADNINNNPLRDVVEGAEGSTKRLPIIGGIEVGAQALVGSEGKIIEKSVVVGGGKETYIGTVASSDGQQMSKEEMKKLNIADPKDVEKLKGNLKKLAGRKGWKLDLVDTPRGREFRGIIEADEEEEGVEKSGSGTESEGRKEGKKEGGGGKQSRMGTGKGKDDEGPRQGDDQDNDEVEGGSEEVYKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAH6</accession>
    <name>A0A9W8YAH6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004889</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAH6"/>
    <dbReference type="OrthoDB" id="3799856at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="434"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="653" mass="73801" checksum="B03B8B3C620AC590" modified="2023-11-08" version="1">MADGHKKRKSSKSPSPDAGWKRVRRAPHSALDHSRQPGNDTRLAPAESAFRGDKDPYFPLQSERAASLSDRDEWAFRLKVISSSTALHHDVFEQAVQGIVRICFPQGVLEELGIDILARHMRAENAPVENIAKMRDIFRAEMIHRITWMLSEYLDTPARVETSVQSIIAASARLHSDKIGSDVLGDVVASLNGLRARVPVNSTERLATNDVFRHVLSDDRSGTVNEASSDSEVEHLEQSPVIKKRKKNRNRSEHYETDEFELNVIEPAPAPKNNKKKKKKKRESDDNSKLQENDQRTDELSSEDIHKYFKGANDVFAFHALPIAKKKLGENAKRKEIRHAMESILADMHDEEYGKWVESHQRLLAGDTGMLVRVEQDLTSHKGAATPAPIEARTPLGKFLGSVKRNFSRRSEHRNRASALSPLDQPSKSEIKTDTSASTATTTAFMHSSTKDNSSPVSIGVQMIDGSMIVTTKEMLKGRLLGHDVPDFLRRTAVKVRADMERKLVPWVVAKLSSFPELMAEPFIFVDMMPWVTCVLDIMCDPHMSMERGNNIAIKGKIFSALEERGLTRVRYIKLRASNHADLFQDTRFGPHGETISWQIGDLLGKSGRSNETRSGYLERILRRLAFTYRKEFPDLKSTLLVRTWERETGQTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAQ1</accession>
    <name>A0A9W8YAQ1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Pheromone processing endoprotease</fullName>
            <ecNumber evidence="14">3.4.21.61</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">KEX2</name>
        <name evidence="14" type="ORF">N0V83_003984</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S8 family. Furin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.61" evidence="14"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAQ1"/>
    <dbReference type="OrthoDB" id="300641at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000005">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF14">
        <property type="entry name" value="NEUROENDOCRINE CONVERTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 8 9" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040951931" evidence="12">
        <location>
            <begin position="19"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="697"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="13">
        <location>
            <begin position="475"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="602"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="776"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="620"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="649"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="778"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="792"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4372210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="820" mass="90323" checksum="528A9EC7C8DC10D2" modified="2023-11-08" version="1">MRILHFWGLLSAATLAAAGKHLPPRNYSSHDYYAIHISPSTSPSELAAHLGLTYEGPFGSLEDHHVFKAPKHQNDIVDDARQELKRRRRKREIGAEFHPLDSIHFTQKQKIKPRHWKRSIIPPRQERAGPPVTDAIRQQREVASKLDIRDPIFEEQWHIFNVKTPGNDINVTDVWLQGITGKGVTACVVDDGLDYDSADLKENFFAEGSHDYNDHEDLPTPKLSDDRHGTRCAGEIAAGRNDACGVGLAYDSKISGVRILSGDITDIDEALAINYEMQKNHIYSCSWGPPDDGKTMQAPGILIEKAMVTAVQQGRDGKGSIYVFAAGNGAASDDNCNFDGYTNSIYSITVGAIDKNNQHPYYSEACSAQLVVTYSSGGGDAIHTTDVGANKCTSQHGGTSAAGPIGVGVYALLLQARPDLTWRDVQWLTVLTAVPFDQPSDWTKTALDRMFSHQFGYGKLDAWAIVEKAKGWKPVKPQAWFYSPWMHVKKEIPEAGQGIASVFQVTEDMLKNANFERVEHITLTMNVEHQRRGDLSVELISPAGMTSHLSTARRDDEAPLRLHRLDIHVYQRPGNLKKGVFTDWKLRLWGECIDASKAKLRPIPTEHDDDDHDKIDDPSAHTTSVVIPSGTAPTMTSLPDDHPDRPVNQKPSQTLANGGQQTTATTAIPTSTASASPSENFLPNPFPTFGVSKRTQIWIYGALSLIIVFIAGLGTYLFIARRKRLSNSRDAYEFEVLDGDEDREDTGMLGAAGAGAGAGARRNRAKRGGELYDAFAGESDEDLLSEDEDDQGPYRDREHQGYNEKYQEDDADAGSDAGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB41</accession>
    <name>A0A9W8YB41_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB41"/>
    <dbReference type="OrthoDB" id="4222821at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="203" mass="22343" checksum="7B5514D54C084319" modified="2023-11-08" version="1">MACIDDGDTYSWNNNPDLTYNKDGNDRTQVGSGTSPTISRDEGKETLTGQLMKLSNRAMGATRELECAVMTTPLTVNSPVVNEAFETADALVRIINSIPLPDSTYGSTQLLSRDESERQLPTEYSPIFLALASHQQVLALFRAICDSIKRSLGSLVQGTESQQQTLHGVDSAQVMLRTLPHEHVKLSEAIQELQVCIEEEFQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCB1</accession>
    <name>A0A9W8YCB1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002508</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCB1"/>
    <dbReference type="OrthoDB" id="5419179at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040979507" evidence="1">
        <location>
            <begin position="22"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="174" mass="18346" checksum="3D2E832191496DA7" modified="2023-11-08" version="1">MLFSGVVSPLLFSILASSAKANIFEERSDRALPFNFTYAFTAQLNLGKPLPPINITGGVLNTEPILNGTVTGPAINATVTGGIATPSIYQNGIIQVPTIQVWGITSEGYGFVINELGIGSPKGQVTRIQLTIGGLSKKYQALQDSYVLATVNPNKEQTKVAVQGYIVGNAASYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCP3</accession>
    <name>A0A9W8YCP3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA (guanine-N(7)-)-methyltransferase</fullName>
            <ecNumber evidence="11">2.1.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Transfer RNA methyltransferase 8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA (guanine(46)-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA(m7G46)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TRM8_1</name>
        <name evidence="11" type="synonym">TRM8</name>
        <name evidence="14" type="ORF">N0V83_003167</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10 11">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Forms a complex with TRM82.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.33" evidence="11"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCP3"/>
    <dbReference type="OrthoDB" id="47276at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106143">
        <property type="term" value="C:tRNA (m7G46) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine(46)-N7)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000060">
        <property type="entry name" value="tRNA (guanine-N(7)-)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03055">
        <property type="entry name" value="tRNA_methyltr_TrmB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025763">
        <property type="entry name" value="Trm8_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00091">
        <property type="entry name" value="tRNA (guanosine(46)-N7)-methyltransferase TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417">
        <property type="entry name" value="3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417:SF16">
        <property type="entry name" value="TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 11 14" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 11 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 11" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="128"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="163"/>
            <end position="164"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4372876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="331" mass="38181" checksum="B1C132DC2D28EB18" modified="2023-11-08" version="1">MPGEPAKRTRRAEYKQSLQNEEQNGALPKKKFYRQRAHANPFSDHSLTYPKSPVDMDWASLYPAYADKNQEQEMNDAENESHDGKEEKQRALKAISRDVEIADIGCGFGGLLFALAPKFPDTLILGMEIRTSVTEYVQEKVRALRVQNASTSGYQNVSCIRANTMKFLPNFFRKHQLSKIFLCFPDPHFKQRKHKARIVSYTLNSEYAYVLRPGGVVYTITDVQDLHEWMVQHFERHPSFERVEKVFEEGEGRMGRVWMIEDLGGVQGDYSRPRHVVGMKANVFHFSADCPCSEPYIPSAGKNVLSSRISFFRRYSSRIQKGFGMNVIWYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD43</accession>
    <name>A0A9W8YD43_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GH18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD43"/>
    <dbReference type="OrthoDB" id="73875at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF333">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH18" evidence="1">
        <location>
            <begin position="23"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="146" mass="16310" checksum="E235356C6E3D4FC6" modified="2023-11-08" version="1" fragment="single">TTEEFCATKKVIHKTCSKDNGNTRVVGYYEGWAKNRPCNVFTPEQIPIGIYTHINFAFGIIDPKTFKIAPSSPDDANLYKRLMLLKQKDPGLKVYIAVGGWAFNDPGPTATTFSDLAASVPRQKIFMESVISFMSTYGFDGLDLDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD48</accession>
    <name>A0A9W8YD48_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD48"/>
    <dbReference type="OrthoDB" id="3001700at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="102" mass="11003" checksum="40770182464FC716" modified="2023-11-08" version="1">MAIGIFTILAGLVALAAGYFYFFGISPETKRKLENQALKTMGENKMSYMAKDSINKIPSSDQEDVKNLKQGISNLAGGTMQNPIGEQAGETTDRLTSPLTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE91</accession>
    <name>A0A9W8YE91_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001893</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE91"/>
    <dbReference type="OrthoDB" id="6359816at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843:SF5">
        <property type="entry name" value="BTB_POZ DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="56"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="347" mass="39411" checksum="D6370E2929B9C33D" modified="2023-11-08" version="1">MRLTEKCAKYCNAELKINRVAGAGDKEVTNPYLLIPLYDRGTDQPLDSLHLSEKHSDFTIKCRNSVYPVHKAIICPRSPYFEAAVRSMKVRLSGNPLKMILIKRQEAEEGAIDLKDVEPELVKLIVQYFYELDYTIAGESEQPPTSVRYLPKTAKGITDVEWRRAFAELLARLNRESMDEAVKVYKEHCSNEPTKDQAYVIDFSVSTEDSILLKLLLITEKAGSSSGTPWKPATLQEAPVIHAKMYSIADMYDVQGLKELAVEKFMATTGDHFTNQSFYDAIEYVYQRTPDTDYGLRDVVAQRLRDQVEIFGMHPKMEQAMKDNEDLALHVSKMHFGAKRCGPDGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEK2</accession>
    <name>A0A9W8YEK2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_002315</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEK2"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494:SF1">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="510"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="540"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="64"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4375229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="707" mass="81664" checksum="74F64CC77B6BFB5E" modified="2023-11-08" version="1">MERSGFHRSKTEPPPPIMVEDTASGEPPQPSPSPTAPARAFSAYEARPSRPPFERGESQPLLRERRKGTFGHRRKHSPNVEWREEWTKDSWKQGRVLLIDYVAREHTHEGRRKIVAQEFCDVDSLRRFYLKEELSGQAALRVIHVQNASWATRFILRKFNIDASDDLVGTTFGRWARYDSPQKRGGKPVLNGKTFRAQRDPWRGISRAAFGCDYLKYYDRHAAMNAPGASGVKPMMELNHFDQADQPCYGYDVHVQRLSVYVQLSDGKPGQAVDPDIPNPYNEEAFQEYQRLKKQYGNVDANEHQDRYIPKLRTLDNGTTIIIFETSQSGSVRDTLIGARQEIESRWRRLTFYLPREDMDNDETLATECMDFILKDIFKAVAYNWDKFLSACETHVGILEDKIYENPADESRAPELWKNSAQWLKVERLLYIHADMVKEMRSHLHDLAQADPKEDEPWLGSVPDEIEKLTGQWERDVMQPTTALSDLMYKSVGIRDARHSLQLGLSMWRLSWITFIFLPLTFTVGFFGMNVNTFSENPPIKWWFITSIPVLVVVIILWYAVKHNLSSQRQNPLRKGFYEAFYHELATDYSTLWTRRGPQPNVVPIGWSGALKWRLMTSWFSNDKLQLGQTYNPATEEFGMWSRTKRYLVRRWLSELAVMPTTALPQTSEPGDHDLASLQNSSMNKDIGAIAEAYADGKVSQLESHEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEN5</accession>
    <name>A0A9W8YEN5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002417</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEN5"/>
    <dbReference type="OrthoDB" id="3779310at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="589" mass="64644" checksum="4565528D715583D1" modified="2023-11-08" version="1">MDSRLIDVDPTSKPTEAELFEMETPKVGVSLSESLNAHSTTPPPLLEDCPSILSDYEDSGTKAEQVSLRSKLELATYEYAALEERHRTLQDQHGDLGRLASDYYDVIRGLSASEDQVASIEQLRAEAGQEGLRAKALEADVFRPVVQALRSLIEREGGLEVLQGQASEADMIRSKVDEVGGPQALSDLVSEVKLLRQKQQEHIELNCTIDELNEFRVKAAKYEKLEQAFNEISNKAQTYATSTSSLLRASQSSTTQKLMMPPTQGNQQQPRSTGISSTMSAPINPARASSIYATPMGDDPDRDLYEPRVPVTTQTQRTGSNTLPLGRPRPAATSVDNSSRRTTLKRKDHDEIPTYSAKRPRVDVGRTSTLVKTVLPGPSTKRLDLATDDWRLARALNMGYNRESSRGGHVQSPFAPRPLISGGMTAPVYSVSRTRGNGRLERYASLSPPISSLHLPGSPIIKTEIDEDERINFPAVPKLAVSLPIALWIGAADPQSISPSSALKTAGDIPSSLAKFLLGEFTKWINKGNNTVWSSMPLNRGACIIGYPRPCALLQEVQGVRTIVFLPLLDELRRGISWKDERYWVVGGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFD5</accession>
    <name>A0A9W8YFD5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_001061</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFD5"/>
    <dbReference type="OrthoDB" id="2151789at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF34">
        <property type="entry name" value="FAD BINDING DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_3G02770)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040720026" description="FAD-binding PCMH-type domain-containing protein" evidence="5">
        <location>
            <begin position="19"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="6">
        <location>
            <begin position="94"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4375784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="532" mass="56774" checksum="3FB6D4DDA98AAF01" modified="2023-11-08" version="1">MKVTTIVSVSALATAALGSTFEPADFNVTKALIANGIDVAAIPELAGLVERSSLSACSIACTTLKIAFGNDSVLSEGTAAYENFTSSYWSAQQNSVDPYCIFKPSNALQVSTVVLLSRLTQCPFAVKGGHAAFAGGSSIEGGITIALEKLNEIKLSSDKKIASIGPGNRWASVYPALEQYGLEVIGGRIASVGVSGLTLGGGISHFANAYGWACDNVDSFEVVTASGIIVTASATSFSDLYWALRGGGNNFGIVTKFNMRTFVQGPMWGGQRAHVESEFDKIYTAYYNIAKDAANDTKQAQIVSFALQGGSKIAAEDLEYSDPTPWPPIFDEWKAIPAIVDGTVIANLSTMVQTLGAGTPDGIQETYWDRTFKLDRDLMKSFIDTFYEMLPAISDAADILPVLSFQAITVPAMESMQQNGGNALGLDPKKGPIFIANLAIMWSNVADNTRIMSFSNSLYESLVAIAGEKGLNNDYIYMNYASPYQDVISSYGPASKQRLEKIAGIYDPTGVFQKLQPGYFKLEGAPYGAFPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFH4</accession>
    <name>A0A9W8YFH4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ribosomal 40S subunit protein S9B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RPS9B</name>
        <name evidence="11" type="ORF">N0V83_002986</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFH4"/>
    <dbReference type="OrthoDB" id="1697570at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000021">
        <property type="entry name" value="40S ribosomal protein S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_uS4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_uS4_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003139">
        <property type="entry name" value="PRK04051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF5">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="10">
        <location>
            <begin position="4"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="RNA-binding S4" evidence="9">
        <location>
            <begin position="105"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="163"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4374245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="192" mass="22137" checksum="0ED6AFEA3A9CE0F2" modified="2023-11-08" version="1">MAPRSYSKTYSVPRRPFESARLDTELKLVGEYGLRNKREVWRVQLTLSKIRRAARSLLTLDEKDPKRLFEGNALIRRLVRVGVLDESRMKLDYVLALKIEDFLERRLQTCVYKLGLAKSIHHARVLIRQRHIRVGKQIVNVPSFMVRLDSQKHIDFALTSPFGGGRPGRVRRKKAKAAEKGGDDDAGEEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFI8</accession>
    <name>A0A9W8YFI8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001408</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFI8"/>
    <dbReference type="OrthoDB" id="10062838at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026505">
        <property type="entry name" value="Solute_c_fam_35_mem_F3/F4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19346">
        <property type="entry name" value="SUGAR PHOSPHATE TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19346:SF4">
        <property type="entry name" value="SUGAR PHOSPHATE TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="288"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="360"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="464"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="496"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="255"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4376127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="535" mass="59074" checksum="9504C78B2D31A894" modified="2023-11-08" version="1">MPESIPLRKKPPPKARSLAHTLSDDFSLEDRSNSPVVVSGESSRQLSRSSSPFTDVPQPRQHKQTNGKGAAFKEPDSPSNSDVEFDDWDEHDAAMPSDLRPSLERGGGRANAPLLGHRKSDDEGIEDGILSRSTHHFHERDPETQAKYETRRRYTYAAFLLALSLVSFTVQTETAVYIQHQLKWEKPYCMLYMTHGSWVLLWPAMLVILRMQRLSEPWPTFWRRHTQLLRQTALMVQHQTLHPTPRQSQVSPVRYMLRMTAFITCALTMAGGSWYVAVNQTTASDLTAIYNCSAFFAYAFSIPILHEKVRTSKIVAVAIAIAGVFIVAYGDISPAKHGSKSGGGAGGDKAPPSHEAENRAFGNLVIGIGSVLYGLYEVLYKRLACPPEGASPNKGVIFANTFGSLIGAFTLSVLWLPLPVLHYMGWEIFELPRGEQAWMMAISVLANATFSGAFLALISLTSPVLSSVAALLTIFIVAIVDQLLPPPLNSPLTPAAIVGGLLIIGAFLLLSWATYKEMDEERRHKLEESPSEMDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFT9</accession>
    <name>A0A9W8YFT9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_002170</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFT9"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000072">
        <property type="entry name" value="Kinesin family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.320">
        <property type="entry name" value="Photosystem II 12 kDa extrinsic protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF9">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="2 3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="5">
        <location>
            <begin position="2"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="399"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="567"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="608"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="406"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="418"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="574"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="90"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4375088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="744" mass="82084" checksum="3CFFC3215860B840" modified="2023-11-08" version="1">MSVRVVARIRPLLKHELDKDTIVTAETIDGETIPAVVRIPSPKNDAESFSFQFSSVYEQDATQQQLFDAEISPTVKQLFNGFDLSIFAHGCTGTGKTHTMRGGKSLADRGVIPRLLSAIYRRCKKIEKDTGGATQVEVALEYFEIYCDRVYDLFEPPEKRTPSGLPIRDNHGKTVVVGLTEKPCTTLKEFEQLYDQANLNRSTSATKLNAHSSRSHAILCVKITQTSESTVHVSRASCIDLAGSEDNRRTDNNKERLVESSAINKSLFVLAQCVEAMNKKQNRIPYRESKMTRILSLGQNKGLTVMILNLAPTRAYHLDTLSSLNFASRTKKIEVAEVENDPILRTMVKPLAATSSIGGATIARQPLRPLTSILNANVPEPEKAKKGDKPVKAFSVYSDTRKPAARSSNLPLQNQSVRRAEGQKRAAEPSSTFASRPAKTFRTADSTSRIRNAEPAMTKASIEALIAQKVDEKLAEKALQDAGTSAPALSAELQKRLDDLEHRIDAKEEDGKSQGLQFLLMAKQHHMRGEDASALRMYQLAQPFFPGNAKLQAKMKSLEDRIRAKKEEANKHMSAPVSSTTTSAPISSLMAPLKADKKAAKPKMVYNDESDHDDEFAPLPASDHEDSYASDDSFKYKAKAARKPKKTSKKLPIFRDDADASLNPGEQTPRTSHLLKIINSRDINQIKALKGVGAKKADNIVNCLVEMNEEEVHDLETLSMLKGVGGKTVDNMRMGLTVSCGYDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFY9</accession>
    <name>A0A9W8YFY9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_001312</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFY9"/>
    <dbReference type="OrthoDB" id="2151789at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF34">
        <property type="entry name" value="FAD BINDING DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_3G02770)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="5">
        <location>
            <begin position="66"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4376032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="500" mass="55514" checksum="C5DD280A783A6D7A" modified="2023-11-08" version="1">MGSIVETSDPGIAQALAKHGVDVERLSKDGGSSAVTLLSSLYPKQIITTATDTEAWRKQFFSQHQYAAKPALIFQPKSAKEVAIAILLCRQNNAKFAVKSGGHAAMKEASSADNGVVIDLKNLNGVELNDDKSIAKLGAGNRWERVFEELAKDGLAVAGGRAGDVGVGGYSLGGGISFFASARGWACDNIRNFEFVTANGDILAVNYQSYPDLFWALRGGGSNFGIVTRFDFETFAQGDIYSGSVLFDYEHKVDVVKAFTSFAYNSDPKSATWLTAAVHEDKKLFSALAMYAEPTEEFEVMKAYGAIPSLHGSQKVRSMASMVRELVDVQAKEHRQNYWNHTFKFDPDFIDWLIEMYYEEVGPHHGKYESKQALVIVMQYYTKESIRQMQREGGNCLPLKEDEAPYVNLLIPSAWVHEKDDELVLGLSRKIMERAVDEGKKRGLFVDFIYMNYGSVYQDVLKGYGKENYDKLKQVATKYDPEGVFQTLMPGYFKFGGAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGC0</accession>
    <name>A0A9W8YGC0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_000269</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGC0"/>
    <dbReference type="OrthoDB" id="4337792at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="3">
        <location>
            <begin position="54"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4377444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="436" mass="49664" checksum="1BC0921AA5942A33" modified="2023-11-08" version="1">MAMRTHMKGDCSSDFALPGNYPLGSMGSARYASLEKVQELLRNYRDNIDTFVARYFEIYHPIMPLLDPSAFQTSMTNFCNSPEQADSDWLVESLVVLGLGAYASSRDETASAEFFYASEACLAKTAYMSQPTPTIIGALCLMVLAKQVAYATCWALDTCWNVMGVVVRLCMMTVLHQEWMPEFQDPMIVKERERRRRIWTVVVYLDIQLSLITGQQSLLPQDTLVITTEMQDPSKLEECWDSTLPQSFPIICHFLARINSYTDQITYDQVLQYDLTVRQLTQHVTKLPGSDILRLTLDIFFRRVLLVLHRRHALDPEAALLYPTSYWTSLDCSLAMMVHHRDIYDMAARLPNIDLIGRLYMLDFFATALTVCIHLLRSDAPLATASTTEGTMPPRQTILSTLKNCLDIFSKDQNKSLCFRTGHPLLKAVFELLPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHG6</accession>
    <name>A0A9W8YHG6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000533</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHG6"/>
    <dbReference type="OrthoDB" id="5863171at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="441"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="462" mass="49108" checksum="FFB00569EDFD8705" modified="2023-11-08" version="1">MDPSIVLNGHNASGNAAPTTSGGANSMPASTPQLTPQSTTPVASAWPSQMSTTPANNSAAAGPSFPQFSASTAAILERVRGNGGHASGSAAFEAKRAEILQNYVTSDKLPTPPPIPSSGRRGRGGRVGTPSALRTEAGASPAGASPARASGRGRGRGRGRARARGGKRKRPDSEEDDHDDDNSDISSSYTPLPTRTKSGRSVNKPVAFVPTLPEPSQGIKRRKSTKTNEVVTVLEKEWLCEPCLRLKRSVVQETQNLVAAQNLSIDEKRAYFSTLSKDQLLGLLLTATIRHPELPIFPPNVQDLIPKPLEKNSEPPVPPTSQPFMQPQYQSSPAFHNATISPPNGHISTNLTPHSHDFSDIDPAKAQLLGEYRSQQPAGQPHPALALAGNQYEEDDGYDTDPPAHYPKPGNGLARTLRPESEDLNWLVDDNFEVFSHGWKGDGTGMGADGTLDSMGSEPKVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHI9</accession>
    <name>A0A9W8YHI9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7580 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001256</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHI9"/>
    <dbReference type="OrthoDB" id="5331891at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056002">
        <property type="entry name" value="DUF7580"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35186">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35186:SF4">
        <property type="entry name" value="PRION-INHIBITION AND PROPAGATION HELO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24476">
        <property type="entry name" value="DUF7580"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7580" evidence="2">
        <location>
            <begin position="51"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="329" mass="38303" checksum="25B8D1D7D060ACFA" modified="2023-11-08" version="1">MKRDWSRKRQNSAPRGFQSADEDNTIDYAESARIGTYTSVIASKGSRSFVSRQDFCARMHKHNQETVENSRLFLGHLELNENESHRVSFSKAYLTFGRSEPISLVQVFRTFFRGRNTESMPHERIGLAKQLASAMLQFQRTPLLYKNWSSNEIMFFGKTQERIEMEEIFRGPHLNVCIQQESQIGYEREKSSKRKGKQPASEAFIVRGSLFELAIMLIEIAFQDELTNLVSPTIFNDAENIPGEEINPHDEKQKQYQARWFAAEKLCDRVAAHLGSDYRDVVRRCLDSCLNQGPNNLQDEEYDAYIYTDVINMLEEIQAWMIREDWEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJ72</accession>
    <name>A0A9W8YJ72_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_000738</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJ72"/>
    <dbReference type="OrthoDB" id="415825at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF39">
        <property type="entry name" value="FAD-BINDING PCMH-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040986360" description="FAD-binding PCMH-type domain-containing protein" evidence="6">
        <location>
            <begin position="20"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="7">
        <location>
            <begin position="146"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="634" mass="69956" checksum="58E36FA6A9735ECF" modified="2023-11-08" version="1">MWLSSAVFTIFVVTPVVFAINFPYESIQLKDSDVGNNSDIAFGKLPVHASPRCKSYPGYESWPSTDRWGAFNVSLGGALLRGIPPAAACYEGEYKDAAACAVVRRRQGDALFAKEDPLIPFGQWQLDNPCPVPSFNVTPPLLECNLTAFPNYVVNASSVKDIQLAVNFARNNNIRLTIKNTGHDWIGRNTGGGALQVWVHRLKAFEYLPSFQVGKYKGQAARVGIALEQHEVYKNMAKANITLLAPGSPTVGAYAGFMQGGGFSYVTSRYGLMADQVLALEVITADGKFVHADPEENADLFWALRGGGPSNFGITTSAIVKAYPPTTISRSDFYIQTGPIFDNSTTSIRVSNETFWKAVNVYFSHNLRIQDAKGIMWNFINTQGPSPWSPERTFTLQSQITKPGITADGMKELMAPLIKDMNDAGIALPNPEPHFWETYADFGTIPGGPTGATSSGRFGSRLIPRSNFEDPKSKAFLKTIASIRSFVEEGGYNFHSVDYAPTIETAGYPGSNSAVSQYMRNTVMHMTGYDNTQYTPDLTDEEWKSSHARLNSYVNKWRDASPGSGAYMNEADTEEPNFQDSFYGDHYDRLLQIKNQRDPWRVFYAVTGVASDEWKVEGTRGLPTQQGRLCKVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJL4</accession>
    <name>A0A9W8YJL4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S33 tripeptidyl aminopeptidase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_000302</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJL4"/>
    <dbReference type="OrthoDB" id="425534at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013595">
        <property type="entry name" value="Pept_S33_TAP-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051601">
        <property type="entry name" value="Serine_prot/Carboxylest_S33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248">
        <property type="entry name" value="2-SUCCINYL-6-HYDROXY-2,4-CYCLOHEXADIENE-1-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248:SF25">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08386">
        <property type="entry name" value="Abhydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="111"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S33 tripeptidyl aminopeptidase-like C-terminal" evidence="4">
        <location>
            <begin position="451"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4377477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="579" mass="62599" checksum="3551233E4B6CA88A" modified="2023-11-08" version="1">MINIPKRLGKVVRKAHRLVRITYAIPFSDSSKAGLSYPTDSPQTNTTSLNWTPCDLDFPPSQQAVIDANGVPIYCATLEVPLDYSNADNKETIQLQLLKIKATNEPSKGSVIFNPGGPGASGVEEVSQKGILYRDVFGGNFDVVGFDARGTGRTMPFACIPSNSTSSTLSRRATNFTIPQADAYSILTSKAWDDAGVFVETCANTEGNADIAQYLSTAFVARDMLNIVDALNEDGLLRFWGRSYSTVLGQTFAAMFPDRVGRILLDSVVRFDDYHSGQWLTANRDTEVALVNFFRECVGAGPEACPLANLTGPSTTPESLHQELGKAFQQLLDEPVILPDSYTPLSQPWWQPGGITLYQELKYWLLVNLYQPAQFPTVYALVDGALKGNGQSVLETLTTTPPSDNATTPEIPWHLGVNAFHGIACSDSPLRADAPEDMYALLRAQAAQGSWSDVFGPQTWACAQWKFDAAERFTGPFTAINTSFPLLMANGAHDPIAPLSGAWETSTNFPGSRLVTHNGHGHGVMNHPSNCTIKAIHDYFNDGTLPEVGTVCEPNQPAYEIALEAAAAASGNNTTEASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJQ8</accession>
    <name>A0A9W8YJQ8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_000620</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJQ8"/>
    <dbReference type="OrthoDB" id="26149at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040144">
        <property type="entry name" value="RAP1GDS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10957">
        <property type="entry name" value="RAP1 GTPASE-GDP DISSOCIATION STIMULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4377790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="643" mass="69954" checksum="1754BA1D5277B220" modified="2023-11-08" version="1">MPLSEPELTEFLESLELLLEGSPEGQNAALRTLLHRLEHFVDTEDTEPVETATGLLGTAVGGQKGWQTPFRESGILSFTLSMLSNVNRTDALVKQCLRVIGNSVADNGTVSHDVIKTMTNCLTNAQDTNRELAVQHLQQIIGCLASEGLRTTALAVVFNLSNGFGRLTRPRWQAMLTKTDPAKAAAATLRLDATISAYLALEQIAEAALDYATDLLTWTTGNLTAAQLKDATSLETFTNLLRTALRYDPDYYDEYVAILVHYLHDPEFQSKVATPKLLDDLVSLLLDFEARLTPAESEAVLIDLSIAKTEESSTSEETNVLLLTQLINSISTISATDAFAQTFNVTSQVVEKIRAKLRATQYSPSTVCACVMLGNLAMSDEVCIDMVNIMELHITLISILSAGTHSSVLYAAAGFMRHLTFPEANRSLLADAGLMQACCRLLNLRDPSVRGEAAAMLCKLVTNNFHNIEKAIYEEDGDATILTRIVEQAIAPSTPLPSTAMKNPMIELGRTIVAVFRYLGRPNAEKDVDAVRDELLKVPILARPVARLIRQRFYADARSEGLLGLGLMAQSPKGAARVVEEINEDDGLLEAIKESAEGKDGGAEQQGSAAGRDYQNAIVLLQALQNNAVSSSRTLLWLQTDVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJZ1</accession>
    <name>A0A9W8YJZ1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetylglucosamine-6-phosphate deacetylase</fullName>
            <ecNumber evidence="2">3.5.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NAG2</name>
        <name evidence="12" type="ORF">N0V83_000485</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N-acetyl-D-glucosamine 6-phosphate + H2O = D-glucosamine 6-phosphate + acetate</text>
            <dbReference type="Rhea" id="RHEA:22936"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="3.5.1.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. NagA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.25" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJZ1"/>
    <dbReference type="OrthoDB" id="10264777at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008448">
        <property type="term" value="F:N-acetylglucosamine-6-phosphate deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006046">
        <property type="term" value="P:N-acetylglucosamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00854">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000065">
        <property type="entry name" value="N-acetylglucosamine-6-phosphate deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003764">
        <property type="entry name" value="GlcNAc_6-P_deAcase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00221">
        <property type="entry name" value="nagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-PHOSPHATE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113:SF14">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-PHOSPHATE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038994">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="11">
        <location>
            <begin position="73"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="8">
        <location>
            <position position="313"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="255"/>
            <end position="256"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="348"/>
            <end position="350"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4377657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="438" mass="46518" checksum="4817145243F8459A" modified="2023-11-08" version="1">MPSVISTKGDYADAQRSSGVVKFTNCLLTKGNELVKEDLWVSSVSGKILNGQEILYEHRTAPEEIVDLGGRILSPGLLDTQLNGAYGFDFSVIPDEGTAAYGKGVLRVNRRLIATGVTSYLPTLTSQPPEVYQKALPFLGASGSARDPSYGSESLGAHCEGPFLSPTKNGIHNASVLRSPVDGISDLSNCYGASNLHPSTIKLVTLAPELQGALPSIQALTSQGIIVSIGHSEATYEEAKVGIKSGASMITHLFNAMRPLHHRNPGIFGLLGTPSSSIQKPHFGIIADGIHLHPTSIKIAWNAHPEGIILVTDAMRLAGMPDGTYDWTNGSRIIKNGALLTLEENGKIAGSSIQLVDCVSNFLNWTGASVPEALKAVTETPARMLGMQDIKGTLKEGADADLVVLELQGGVEGEEKKLVVDEVWKFGIKVFDKSSAQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CGN0</accession>
    <name>A0A9W9CGN0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010551</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CGN0"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF154">
        <property type="entry name" value="OXIDOREDUCTASE, SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY (AFU_ORTHOLOGUE AFUA_2G04560)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="313" mass="33961" checksum="93C357B7FA4A5BD3" modified="2023-11-08" version="1">MQGATGFNPARDIPSLTGKVVLVTGGTAGLGAASVQALAPHNPTHIYFTGRNVSAADSLISEIKGKHPSAALSFIELDLSSLRSVKEGLANGFKHDRLDILMNNAGIIAKPAILSKDGYEIQFATNHLGHAMLTKELLPYLLAAAETPGADVRVVSNTSDGYAFHRMIKGAISFSELGSTMSRTMLGPWIRYGQSKLANILFASELARRYPNITSVSVHPGVVNTPMNTEMKGFNKAFVDFTTWLARIPKWEPDQGVLSQLWCAAGAKQGELRNGGFYRPVGVDQWETLTKEAKDEKLAKELWEWTEGALSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CGV6</accession>
    <name>A0A9W9CGV6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_010112</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CGV6"/>
    <dbReference type="OrthoDB" id="1902587at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4362995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="130" mass="14123" checksum="0884B6331AC1028B" modified="2023-11-08" version="1">MGVEKVVLKEGNGLDIPKKHDEVSIEYTGSSNSSRAKLHVSPNLQVGCLTRQQRITKATSAYERSHSRADLFYLRFDSSVGRGDLITPIGVGRVIRGPFTPSSTSYMISSQAATYAPKCANGLTLLHRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CGY0</accession>
    <name>A0A9W9CGY0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sec39 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_010694</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CGY0"/>
    <dbReference type="OrthoDB" id="3434013at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013244">
        <property type="entry name" value="Sec39_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40787:SF3">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40787">
        <property type="entry name" value="SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08314">
        <property type="entry name" value="Sec39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Sec39" evidence="6">
        <location>
            <begin position="16"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="701"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="877"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="715"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="889"/>
            <end position="901"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4361754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="947" mass="105227" checksum="A9CC2AFE4A1673D0" modified="2023-11-08" version="1">MATLRKLQTLSGPHCVLLAAQYATEANVTALRALTALRDADLPLELTLSILLTYLPEETEPSTYLEYLNELATNSRTPGEDPAASIVLSSVEELSNSRAKKRRRELELLPVVHPLYAAETELDLLSHFLIHRAHRIDSQTGLLDVLPQLLVPFLSRSEYLRTWFISTVLPLLRLGYEYYPQSTTPPLEDFARLKGKRAIDYQLSNARHASGTDIYNLTRDLKGVVAPWICGANDRKRRRITREGRRDSIAQEHPQEPDDWTCLFQWLLHTSKNDLALVASAFTQWDGPEDMDLGGYEEGRDYVDEEQQRRLEVKYAQTALACLYLVEKSDISSLQIAHSLLRRISELLNYDPPPNLDTAVDSLPSYNLKSPLLQDSTTSLLREERLLEPENVITKPGRESVRILELVVFSACALSSLQHPLSPREVAKLSLRDDYAEQFSLLQKILRALSSGSKADGNQWATIRAKLLWLWNWGTDQHDNDRQAQGILGMLDRKTLETEILKALVESHHFPLAIELYVKPAFGQQPLLASDVEQVILSSAMNHYDNASNGNRTRGGMKRAADIITAFAPHFPSSPRFQRMHALLAATHAMSFYSLILQHGVPFQPVNIRVSPNPLSLIGKLLSQNRASYTKLDDLISIGQNLVVAMPSTIMDEDQETTPLDAGVVRRKKAAAERRVIGMAIEAALEEDDFETAYSYVVNRLTPDTPSPTPSTSSQRFSFGSLDSENQEDDAEDVAWRAALRAGRYSSSLSTSRSWSYTGSAARPDLRRLEQRMELLSQALLLAPPNHLEEVLKVWQQCESEMTDLLAKETAAEQRFNDAADRKLPGAFDLDSVTVQPQRREIGRGAVEEAPMGLFDVARGAAAAFSKTAFPLRGGAQAAARAEQGDSNGGSSRVSMDFSDSGSHEERVRKRDMVAHAATGALATGSGALASGIGWMLGAKPVTEQER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI48</accession>
    <name>A0A9W9CI48_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dolichyl-phosphate beta-glucosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.117</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ALG5</name>
        <name evidence="15" type="ORF">N0V83_009773</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-alpha-D-glucose = a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate + UDP</text>
            <dbReference type="Rhea" id="RHEA:15401"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.117"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15402"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.117" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI48"/>
    <dbReference type="OrthoDB" id="3784at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004581">
        <property type="term" value="F:dolichyl-phosphate beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04188">
        <property type="entry name" value="DPG_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000143">
        <property type="entry name" value="Dolichyl-phosphate beta-glucosyltransferase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035518">
        <property type="entry name" value="DPG_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="14">
        <location>
            <begin position="128"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4363479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="416" mass="46210" checksum="92DDD0462A0F369C" modified="2023-11-08" version="1">MSKENRAFDTQKLGAGYGFESHPKRAVEMPPTWALALGLGFLLLALCFWVYLALNLLAPSPRPPRRSEKQYITVLPDGSRSKPNELSCWLDKWAAEKEAAKKAAAKEPGTKAVIPPQMPIEEAELFMSVVVPAYNEEARLEGMLDEAVEFLESEYGHAHDGENGQRPHKGWEILVVSDGSSDRTVDKALEFAKEHQLGQHPIQKRGPWSKNGYAKALHSTHIPHGSIRVITLEENRGKGGAVTHGMRHVRGQYIVFADADGASRFSDLTKLVAGCKSIEDEHGRGVAIGSRAHLVGSESVVQRSALRNFLMHSFHILLRVMTPPATARIKDTQCGFKLFSRPTLPYIVPYMHSEGWIFDVEMLMLAEDADIPMIEVAVGWKEVLGSKLNVIWDSIGMAWGLALLRACWILGIYQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI58</accession>
    <name>A0A9W9CI58_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sorting nexin, cytoplasm-to-vacuole targeting pathway/endosomal sorting</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ATG20</name>
        <name evidence="11" type="ORF">N0V83_009804</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sorting nexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI58"/>
    <dbReference type="OrthoDB" id="289314at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06867">
        <property type="entry name" value="PX_SNX41_42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044106">
        <property type="entry name" value="PX_Snx41/Atg20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051079">
        <property type="entry name" value="Sorting_Nexin_Autophagy"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46979">
        <property type="entry name" value="SORTING NEXIN-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46979:SF2">
        <property type="entry name" value="SORTING NEXIN-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="10">
        <location>
            <begin position="79"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="235"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="444"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="60"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="240"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="260"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="444"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="466"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="482"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4363509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="648" mass="71807" checksum="9E3D0FD725D74DF9" modified="2023-11-08" version="1">MWDDEDNNPYGSFARHDSNTSDTPGLASPAARKAPTCCVEYAEAFHADCLQVPYRPATPPSETSSPAQESPEYVSQRDMSDVDYNEEHEGEDATPPKKGGYDARVEQWLYEHPDQLVLITSAGKDGGLEVKRRYSEFASLRATLVNLHPTLIVPPIPEKHTMADYAAKPTKAKEDAGIIELRKRMLAVFLNRCRKMKDVLEDGVFWRFLDPYTSWNDVLNSPPVTNIPKQILKAPPLDTANPTQAHSYLPVPSSSARLKSGGPTSSSGTPVSPASNAALPSVAAHSTPGPQVFGRFPPESRELSEEDLDPYFVHFETSSKELENLLQGPMEKVNRRLLMHLGNWGEDMADLGARFNAFSLSEQSQSLAAAIEKTGQAVDSTYIATAELSSSLSASFSEPMRESAQFAGVVRSVLRYRVLKRIQEDMTKDELEKKRNLLESLERSEAEAKRLEQHLSGAGHGPPTRSRATSNSSQRSTGSEQRQPEEDRASIDSDFPPTHGDQAPSSAQGAPDNGISAPNSPHKKSPSGNFVTNKLFGSITHAFRGMADVDPEKTRRDQIGKTRDSLVQLEQALGVAEKDTKDASAGVMKDLRRFQEEKEDDLRRYMMEFAQCHIDWAKRNKAAWEEAKAEVQNIQHHVEEYPRNSSEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIZ4</accession>
    <name>A0A9W9CIZ4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1996 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008748</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIZ4"/>
    <dbReference type="OrthoDB" id="74764at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018535">
        <property type="entry name" value="DUF1996"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43662">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43662:SF12">
        <property type="entry name" value="DUF1996 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09362">
        <property type="entry name" value="DUF1996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1996" evidence="1">
        <location>
            <begin position="24"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="310" mass="33342" checksum="586385AFCBCFD8DE" modified="2023-11-08" version="1">MLAQLASAYTQVNVDSPFMQKNIDPIVFPGQYSKSHLHSFYGSDAVTINTTTSAELQKGCTNAENPNDLSVYWAPTVLYTTDSGKTYKPVPVARFSAYYNLGETEAEVAIPQNLKMLAGSATATTQAAMIADAQATWFCEGSDSSALDANGFPSSTCSTHLQQLLYFPQCVNVDTLETAYKVNKACPAGMKSMPQLRFSIRYDLRKALPNGWSGTAPVKLACGPAWCSHGDFINGWTTEAAENMIATTKEKQHFSAVNGALGAYNAGPTCTSADADPNHGTSDYAASVAAMSKRSVSAWGWETRSRFVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ51</accession>
    <name>A0A9W9CJ51_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ51"/>
    <dbReference type="OrthoDB" id="3792603at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="422" mass="46613" checksum="8ECB6ABB005FDF2D" modified="2023-11-08" version="1">MNPRPDSPLEAANSLFTRARLRLAAITPPPPNVSTGTGHPQSAANIQGGSPQTGSVPGSGTAPSNGPPLNASTPPVASRNTLTATAFSRPLTASTRVPISAFANSSKNPSITPTPASKDSSTPVIAEETGTSTKTWTRMKPLGVMETLKRPLRSDILSVPKENEPLFDDEEKWWYLGFCELWEDFPQQVINFYKSEDFLDAIDETKGIPIPAPRRTRLSGNPHPSGLLTDYFQREVLEVAKNIYNRVLQGIEVDEGELPTRISLVDAADEDLGNDDFAWNPDFVVRVTDKGDEETHIIGHAEYLGGKPGALSWAIENMVNNPWGGLRCVLGDMAQWMMMGNIRYAFLTSSDEVIFLKFDVTERVKYVNMSTHGEQPVFDPVDLFKEPWIGFSRPIKFTDVLDEAKGTVPWMIWDHRVCEDNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJJ0</accession>
    <name>A0A9W9CJJ0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 41</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">VPS41</name>
        <name evidence="6" type="ORF">N0V83_008417</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="244107at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000510">
        <property type="entry name" value="Vacuolar assembly protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057780">
        <property type="entry name" value="Beta-prop_Vps41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 41 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23411">
        <property type="entry name" value="Beta-prop_Vps41"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23556">
        <property type="entry name" value="TPR_Vps41"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00299">
        <property type="entry name" value="CLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vps41 beta-propeller" evidence="5">
        <location>
            <begin position="166"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Vps41 beta-propeller" evidence="5">
        <location>
            <begin position="382"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="3">
        <location>
            <begin position="908"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="128"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="559"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="565"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4365796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1324" mass="144516" checksum="0B5AA7ACF5035AAB" modified="2023-11-08" version="1">MAADNNGDELRTPARDEARLARVASAEQDSAEQDSEDDDDEEEDEADEEPKLKYSRLTSNLGPVYRNGDATSTFMVAGGQNVGTHNGNIHALTIPSFQLLRTYNAHPSASITSISVSPLLASQPFVPTASAVTDQPETPSRQAAHHQGHASSPRTPRQAQVPAIPSNQIYIASSSIDGHVCVSSLVDSKEVTLRNYGRPVQAVALSPDYKDDRTYLSGGLAGELILTIGAPIGAKSNANTSSASQTAQGWLGAIGLGGHGGKDIILHSGEGTIGAVKWSLSGKFVAWSTEHGIRIMRTNLHLDSADSDVAWKRIGFIEKPNRRAWQDMAGVWKARLEWVDDRSLESDDDAIMSMNGGHATGKAEATLPHHRINQHVQGTKKKKTEKLVIGWGDAAWVVHVQPGGAGVGRDIGERSVGSAEVVHFLRFDDCVVSGISLYTPSLLLVLAYRTRDDDDNPITGADTTPRRMHRRTNGLSPELKLIDAATSDEVEVDSLTVSRFESLSAADYHLGTLYVPHPKTMTPAQRSALEAIGGGIWDVGAGAARIFSTGANVINLPFGSDAKTPSRAPSMSSASAGNASASASSKRQLQIPPNAATAGLKVFIQSPYDCVLAIKRELSDHFQWELEHENYKDAWELLEDHPEIISSTSQLPSEASPASTPVKKQGSLQDFFADDSESQTTVSASKAAHNSAVEKEKRRIGDLWVQQLVNAGDWKQAGKVAGRVLGTSTRWEHWVWKFAQTNHFNEISPYIPKKPIQPPLPSMVYEVVLGHYIIHDRIRFKHLLEEWDPDLFDINSIVEAVKSKLSAGDVTEDSIEGDVQGRDWRILQDGLAKLYLASGRQREALRCYIRLQNADAAMSLIRDFHLVEAVRDDIPGFILMGVSKEQFKSAPLPELEEASSEAINVLVEEGCRGTIPASDVVSQLQKREASFKPFLHFYLRRLWKPEMHERKGTTAKERVAEDRLAADGKIVAEEFADLMVELFAEYDRPLLMEYLRQSQSYDLNKATAECERRDYIPELVHILSKTGQTKRALYLIIEKLSDVSFAISFAKEQDDPDLWNDLLEYSMDKPHFIRGLLEEVGTAIDPIKLVRRIPEGLEIKGLRDGIGRMVREYEIQHSISEGVARVLRGEVAAGMDTLRAGQKRGVKFNIIPCDDEKHDHGKDDHIDIETKGVNPAIVAKVTAQADNKTTAVSSTTNAPQVDPAPPEDIEGEAPPGHCTGCHRPFTLPTAEEEGIVDGPTPLSRDTLVGFACGHVFHLSCLLPKTSASASVATTLQQTLANDAAESGGWSRSVGAKVAHAHVIKSAVGRGCVVCREREGMVGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJL0</accession>
    <name>A0A9W9CJL0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Membrane fusion mating protein FIG1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008298</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJL0"/>
    <dbReference type="OrthoDB" id="3550957at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000747">
        <property type="term" value="P:conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033481">
        <property type="entry name" value="Dni1/Fig1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016509">
        <property type="entry name" value="Fig1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28092">
        <property type="entry name" value="FACTOR-INDUCED GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28092:SF1">
        <property type="entry name" value="FACTOR-INDUCED GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12351">
        <property type="entry name" value="Fig1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007138">
        <property type="entry name" value="FIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="269" mass="28928" checksum="7E4336B52ED3073A" modified="2023-11-08" version="1">MAGLRFAVSMQRLIPFLGYHHVLMILIAVAIILLSLLLAGCSSTSPLIPGIFLISLWYEKYTPAYSTEQVDPGVTQAIANIVGNAQLGVRVGYFGICINRDGGGYICSNNATALVDNLSVDQDPLNLVWVAATFKDAVVFPYLLIVAIILAFFTFILLATFPGWHEERDERTGSDVDVKPFPSRPVSQVALALIFVASIFVLVSVLWQHTASVAAATIAQDLGNGSVKSGVGTSAMVLGWFGFVLLIIVTIGLLVMILSIIVLDRLTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJW2</accession>
    <name>A0A9W9CJW2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_007951</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJW2"/>
    <dbReference type="OrthoDB" id="2151789at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF13">
        <property type="entry name" value="FAD-BINDING PCMH-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040738796" description="FAD-binding PCMH-type domain-containing protein" evidence="5">
        <location>
            <begin position="22"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="6">
        <location>
            <begin position="65"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4366315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="522" mass="56281" checksum="B8C82CE2D8C818B4" modified="2023-11-08" version="1">MQHFICSLLTALLLGAQSTTAAPTNETSQACTEIKNALPGRVLTPGLLEVEYEYETQQYWSTTLRTIDPACIVQPESATDVATVVKILNKYPTVKFATRSGGHDPNVGHATVQDGVLIAMTNLTGATYDAARNVAYVLPGGEWNDVIGDLEPSGVTVAGGRLGLVGVGGLLLGGGLSFLSAQEGMAADNIIEWETVMANGSVVNVNAAEHPDLAQAMRGSGSQFGIVTKYTAKVHAIGDVWGGSCVYDATEDNKLYAALHNFVANGDEDPKAAIIFTSLIVTGGVTTNLVYYFYDGPTRPASGPFADFFSIPGLVCAPTTQKYSELLRSNGEPVRLLNARVSFRVSSPIPSSHPLLTKRKTYTIPYISTRPQMYAEIRNELANITAPFLTTLRATQFSVDFQPLPSLFGRISQSKGGNAIGLTASDPDRLILEIQGAWSLASDDAYAYSLTKQLTDWLDVQVPLWLEEAGMSRDMYLPFFINDAAGDQDVFKTYRDYDKFKALQKSVDPNGLFSGRAGGFKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJX0</accession>
    <name>A0A9W9CJX0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcriptional regulator of ribosomal biogenesis proteins</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SFP1</name>
        <name evidence="8" type="ORF">N0V83_007772</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJX0"/>
    <dbReference type="OrthoDB" id="3269380at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051580">
        <property type="entry name" value="ZnF-Chromatin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23057">
        <property type="entry name" value="JUXTAPOSED WITH ANOTHER ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23057:SF0">
        <property type="entry name" value="JUXTAPOSED WITH ANOTHER ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="627"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="688"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="70"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="177"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="343"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="467"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="88"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="206"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="349"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4366137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="752" mass="81214" checksum="BAECFFAB302EB01D" modified="2023-11-08" version="1">MAGSSKEQRSVPAQREAVLINPPAPALFTVRLRFDTGATLAAAQPSTPPRLLGFAHSTLASLAITPRHTAKLDSHETTPPPLSHSPRESTAASTPITTPVQQSVLPHFGRSKKDSSATVVSAWHRKPHELVAGHLPESDLFFEDSGDCSFPLFPRSPQHQALPRDMAQTASPIDIQTPPRFGSNSPQNQTSNLTSALREAEAQPDLGSTPNQLNPNGFDFSNARPGMGDRHGSISMLGSSFYGNSGARPISMKDRPRRESNNMGSFAGGMSWGGVSVGSWIRDDIMMTGTSPAPFVNNSPSFHSSSYLPKMEAAFMKDFTCCGLTLASLHDLLQHFEETHANAPAARASQPTQNGLPPTSGASATSIAPGQTQGEPSMSTQFGFQPRSASISGSRQRLGSVSRSNLSTVQDVDSLEDMDMDDINFDSLGPIEEAPTQFPVQQTAFNQNQNQLPQLNVNLANTMQNHQGLRTSTPSTPAASQQFNLQNNPTVSSVNTPTLGTVPMQNTHNLTSPESSHPGTPAELDMDFNNFNPMMGMDMGMGMNMNFGNGNFGMSGFDNNGTIDQPGKRLFSKQGAGLNQQQLQAALKNYQLGGNDQSELARRLREQQVLNGASIPQFPFPEEVKPFRCPVIGCEKAYKNQNGLKYHKQHGHQNQQLKENDDGTFSIVDPLTSIPYPGTVGMEKEKPYRCEVCGKRYKNLNGLKYHRSHAPHCNPELALQKLGLGGNLQNLQNMQNANVAGAGMGGIDPSMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKK6</accession>
    <name>A0A9W9CKK6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EthD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006772</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKK6"/>
    <dbReference type="OrthoDB" id="4892971at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4368415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="10183" checksum="E7A987DFEE85FBD2" modified="2023-11-08" version="1">MAPSTNITAVVSYPSKHPESGEALKFNMEYYLSTHMPMIEKTWVENLKTAFEKGAATTMPDVEKFSNVFPAVWVGETGKSNVLSGGVAEGIARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKN9</accession>
    <name>A0A9W9CKN9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006364</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKN9"/>
    <dbReference type="OrthoDB" id="19690at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="50"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="172" mass="19407" checksum="5D7FD82CF2AEF7CC" modified="2023-11-08" version="1">MTSRVHLPNFQTSSLVRRPFSLLNPSARSHTNRLFDTIRQPNDLHTLTLLNAADNRSLITFWSAPWCSTCQEIKPLVKRMIEEERVGEREGGLGFVEVDMSSVLIEDLPVRYRISSMPTLLAFSRQEAQFETKVTRPEDMRTEGFLREWLVSEARRGGRKGGGGGGCLGGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKS0</accession>
    <name>A0A9W9CKS0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial glycine transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Solute carrier family 25 member 38 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_007023</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mitochondrial glycine transporter that imports glycine into the mitochondrial matrix. Plays an important role in providing glycine for the first enzymatic step in heme biosynthesis, the condensation of glycine with succinyl-CoA to produce 5-aminolevulinate (ALA) in the miochondrial matrix.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>glycine(in) = glycine(out)</text>
            <dbReference type="Rhea" id="RHEA:70715"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Mitochondrion inner membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the mitochondrial carrier (TC 2.A.29) family. SLC25A38 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKS0"/>
    <dbReference type="OrthoDB" id="1924968at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015187">
        <property type="term" value="F:glycine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904983">
        <property type="term" value="P:glycine import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000103">
        <property type="entry name" value="Mitochondrial glycine transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03064">
        <property type="entry name" value="SLC25A38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030847">
        <property type="entry name" value="Hem25/SLC25A38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46181">
        <property type="entry name" value="MITOCHONDRIAL GLYCINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46181:SF3">
        <property type="entry name" value="MITOCHONDRIAL GLYCINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10 12" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040863558" description="Mitochondrial glycine transporter" evidence="13">
        <location>
            <begin position="19"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="2"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="110"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="216"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4367440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="318" mass="34773" checksum="DAA493E3EC277604" modified="2023-11-08" version="1">MHTRPVHFFAGLSSGVLSAVLLQPADLLKTRVQQSRSNTLYSTIKSIANGPNPIRQFWRGTLPSTLRTGFGSAIYFSSLNALRRRASLIAAGRTDVVVRGGEHSSSLPKLSNTANLATGAFARTWAGFVMMPITVLKVRYESSLYSYNSLFTASRDIFRTEGLKGFFAGFGATAVRDAPYAGLYVLFYEQSKRKLSSLATKIEETSGTSTKLSSSTSAGINFISGIAAAGLGTTLTNPFDAIKTRIQLMPDRYGNMVHAAKKMLAEEGTRSLFDGLGIRIARKAMSSALAWTVYEELIRRAEREWKEVVEEEKDVRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKX0</accession>
    <name>A0A9W9CKX0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glyoxylate reductase</fullName>
            <ecNumber evidence="3">1.1.1.26</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">GOR1</name>
        <name evidence="3" type="ORF">N0V83_007175</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.26" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKX0"/>
    <dbReference type="OrthoDB" id="9991913at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047964">
        <property type="term" value="F:glyoxylate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF269">
        <property type="entry name" value="HYPOTHETICAL D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="2">
        <location>
            <begin position="2"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="213" mass="23243" checksum="41D0494021D8290C" modified="2023-11-08" version="1">MFLILGALRRLNVPMTTLREGNWRGHAPPPLGRDPEGKTLGILGLGGIGQNLKRKAEAFSMRIIYHSRSKLSEHVAGNAEYVTFEDLLARSDVISINIPLNADTRHLISTREFQQMKDGVVLVNTARGAILDEGALVKALDSGKVASVGLDVYEDEPNIHPGLKANPHAILLPHMGTWSVETQAAMEEKTIANVRRALETGSLRDIVPEQACL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL28</accession>
    <name>A0A9W9CL28_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_006397</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL28"/>
    <dbReference type="OrthoDB" id="270167at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF73">
        <property type="entry name" value="HET DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G01020)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="530"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="3">
        <location>
            <begin position="84"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="492"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="598"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="502"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4368042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="620" mass="69668" checksum="26ACB43D2F7DF7B2" modified="2023-11-08" version="1">MQRDTNRKFGEIHDTKENLIQSIEHIIDVALVKPDEVEPLKLRFVTPLHIPTDLSADNLDWPAYDQSTDYRLTSTEEHIPSLEYISVSYCWKHTQSPDGIHVPDYRISEGSEWRSIRCPPLVFHRAFQFARRHGCAHVWIDQECIDQNDPADIERHLQIMHRVYRESKWTVAALSWPIRNCDILEQLIKTIWTLDMASVESHEKHNDRNQIILQGLESLCNIIYNEPWFRRTWAFQEKLCAQSLQLLVPLGPKLCIPDVFNPLIIGNDLCLPTTKIGHRLDDLGYGPFGVRLKTNWHAKKGWTRPFSLNMKNLYLWLGWNHDSNMLLNYDKLSEASEWHGYFKVILSTMEECDNLIVADRISILGNITQVENRFLSNRLDSSMYSFSTCVLALILANHLPDAVERAKSMSDPGWNPLCYDLGEVMSGVCDAASYSPNQCSGAIKQRILNVTYNATTSSTVFETSTALYIDGGELLAEVIEAIYREGDFPSSVAPTTSTQLHTPSQTSLPPTSPATTTKAQTEGLSTAAKAGIGVGASLGALLALAALGFVLYRMGKRRAARHEQSRADSWAATGVKAELHAESVEPRELAGTMVQEMDGDTGSRVELQGSTPIKTVEGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLI6</accession>
    <name>A0A9W9CLI6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_006730</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Mycotoxin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLI6"/>
    <dbReference type="OrthoDB" id="1844152at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11041">
        <property type="entry name" value="CYP503A1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46206">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46206:SF5">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4368373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="521" mass="58525" checksum="D095FF143CF92497" modified="2023-11-08" version="1">MITVGMDVFSTPFPVVEALGCALGGALVTLICCYQTISLPRGPLSWIVDLYLRWKFLLCGAPMMSEAYTKAKGGAFSVAGPDHDHVFISSKQHLKEIQRAKKDELSFFAATRQMFQPAYTMLGHNWLDERGAEGIGYVKAVGTLLPERMRDIMPNMRVNIKRSFDREFSRVQSASDGSKALPAPALIKKVMCDLNGYAFFGDELAQNEVFMKNVLEYNELVVIAAEVLRITPNFMKNWVGTFFKNQGGIQATVFTMINEVVERRLQEKADREAGLISTPAPSDLVTWIIDTAPKELNWTARRVTYEVIAIWFGSVHALSATTSYALYDLCLYKEYLSPLRDELSSAEFSKFMQTTQGLPLLDSFIKESTRHNPMEAMSGRRQALKDFYYSDGTKVAKGAWTVVPAKAILHDDQYFPDALRFSGFRFVSESAVVPEGVRKALQPEGPSKLTDISPHYHSWGIGGVVCPGRFYASVAMKLVLEHIMVNYEVELVDERAERASIWRSYVLPAERTQVRFTPRCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLK6</accession>
    <name>A0A9W9CLK6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Polycomb protein VEFS-Box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_005355</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLK6"/>
    <dbReference type="OrthoDB" id="166746at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21552">
        <property type="entry name" value="VEFS-box_ctSUZ12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019135">
        <property type="entry name" value="Polycomb_protein_VEFS-Box"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09733">
        <property type="entry name" value="VEFS-Box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Polycomb protein VEFS-Box" evidence="8">
        <location>
            <begin position="383"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="560"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="357"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="560"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4369593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="667" mass="75559" checksum="7C1018E9D4D59A65" modified="2023-11-08" version="1">MVAQQKSKGNFFLEIHGFKPLHKEEPSSRWSATARKSRILFSVKSSIQVSIFPRSSDIPCKTQEAQQATVKGIDSDHQKSTIIDTDHFFIKPETLSISGASKPLSFDKSYKLMISVNFASGNDAEEVYRHLGIENAEQNAHTRLSTMFENILECPQETIILPLRDFKKRRLDFGIEVSMYWTSTGESILSVHNQQTQMRSQPDSYPTPPLDIEKPRYQLTFVYAKETIRRTSLVCPHQSCYKRKPTSIGDLRMHLDSWHDYFRYRAIREKVDEGVEHWRFECEVSDHKADQRASDRADEPFDVRVIAPKQPFNQRKYLAEGNDEYQRAARLERPTKYPISKVAAAITPSIPNPIRRKPPEEVQERPVRAKKRYPVPAAPPGITFFRSSSKRPLREGELISESDDEVDTTWVELRKTAEDQKNKGISNTARRFLNVFDKFMGDEHLQSDIHASDALVRFAREKGAWLWQEQVIYEFKTKMNELLEDEIISNEVHAACLGIVQSQKSIEKEATGQETNNEEVTLGAEENAPEANGEKPPGQETNLSQKLTEIVVQRLRTLSTFMPSSGSGSTKDTNQKGKGKAKLTDTGHLTPITADSDGDVEMREASLKHHHHAADAQQTISQDSDREVDPPYDLCFCGSDALVVSKGEIVIVCNNIVSTPPSNLVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLQ4</accession>
    <name>A0A9W9CLQ4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_005506</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLQ4"/>
    <dbReference type="OrthoDB" id="10253736at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322">
        <property type="entry name" value="PKSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322:SF736">
        <property type="entry name" value="RETINOL DEHYDROGENASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4369743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="226" mass="24818" checksum="69DA9F77377AF947" modified="2023-11-08" version="1">MSVGRIKLARQPKNEALSSPLLAYPNVHFFACDITNPTAVYSTAEKIKTSLNRDPSILINNAGILASHTILNTSDDFLRRIFDVNVLSNWYTTKAFLPAMLTQNRGHIITIASTASYIGVAGLADYTASKAAILAFHESLNQELKHHYKAPNVLTTSIHPNWVRTPLLGPVEQELQRRGSEVLEPEEVADRVVEAVRGCRGGQVFLPKSVGKISALRGCRIGCRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLT9</accession>
    <name>A0A9W9CLT9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_006264</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLT9"/>
    <dbReference type="OrthoDB" id="4074350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05471">
        <property type="entry name" value="pepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034164">
        <property type="entry name" value="Pepsin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="423"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="4">
        <location>
            <begin position="30"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="462"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="488"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="513"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="589"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4369180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="599" mass="64317" checksum="17B908EB138C830B" modified="2023-11-08" version="1">MGLAARATASPEPYVVPPSQEFDGNDGSWSTFKISVGTPGQDFRVLPSTKGGVTYVIAPEGCIAGVDPSDCASLRGAEIFQSSQNIGYQTNVSTSWSAIGQYDVDLEDALNYTQEGLFGFDKVSLGGAADSSSLSLDHQVVAGIADPSYWMGVLPLGQADSSFSSLSQSIDPLLYQLRNNTKIPSLSYAYTAGAKYRLKSVFGSLILGGYDSTRFKANDNDFSYTFSTDPSTLLTVGVESIMATNTLRGSFSLTSGSHFSVIDSTVPHLWLPQDVCDQFETAFGLTYDPVTDLYRVNDTVHAELLSNNPTITIKLVNSLQDSSTNYTNIQLPYAAFDLQASYPFYTNATNYFPIRRAANDTQYVLGRTLLQEAYLIVDYERANFTVAQAVFPDPLPSANVVTINPPNSKLSNGNKSSGLSTGAIAGIAVGAAILLLSLAALAFFFFRRRRRTQKQRYELAGNQVSETGSHHHLTAGGAPLKSPGPQELSGTPLTELATPQNEHFPTNHGYPSDSKTFISMSDTPQELHGESRTPITPRWQEVYMPLRPPGQQLHDGDGGGGSNGSRSVSRGPSDDGFGTGELGPRSGVSPLTGQFQEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLY9</accession>
    <name>A0A9W9CLY9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tuberous sclerosis 2-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TSC2</name>
        <name evidence="4" type="ORF">N0V83_005372</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="19311at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033596">
        <property type="term" value="C:TSC1-TSC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032007">
        <property type="term" value="P:negative regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11210:FF:000007">
        <property type="entry name" value="Tuberous sclerosis 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11210">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035974">
        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000331">
        <property type="entry name" value="Rap/Ran_GAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018515">
        <property type="entry name" value="Tuberin-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027107">
        <property type="entry name" value="Tuberin/Ral-act_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024584">
        <property type="entry name" value="Tuberin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10063">
        <property type="entry name" value="TUBERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10063:SF0">
        <property type="entry name" value="TUBERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11864">
        <property type="entry name" value="DUF3384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02145">
        <property type="entry name" value="Rap_GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03542">
        <property type="entry name" value="Tuberin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50085">
        <property type="entry name" value="RAPGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rap-GAP" evidence="3">
        <location>
            <begin position="1356"/>
            <end position="1595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="475"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="515"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="830"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1135"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1611"/>
            <end position="1630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="43"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="479"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="515"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="537"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="848"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="862"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1135"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1615"/>
            <end position="1629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4369610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1656" mass="185419" checksum="39A878DBC95856F8" modified="2023-11-08" version="1">MSPPTENVPRTPDRRSSTQALFTAFRSLTTGRLKSPTPPPPSTSSATADPARTPSLASTCPGSVASPQSADGTLPRTGQISTGPRAAKPVTGGPPELHGLIARLDRSHSFSERAPAVDKICQVLAEYPVQNILGLWSVASDLLLPEQIDEVADAGYKLLKSCASLSDLSTIERNVFFDAASLRKSDSSFHKRLDIVAELTRGGRDIEACESSIVPFLLTSLDTCFQASRDASNVNRKTHSKRSIDKPLQETENLALMFQYVTDICKFNAKIFTDDDLDLLLRRAVFICQNTTQESDIKNCIKLFDTIITFVHVPKQSLRPCLEVLCAIHKQMFSLQQQTWNTLSNLFKSHVGQAAISCLLHALLDTPEQESLHESSRKSKQHNLYRGTIQVLQLLLFEDGRNELPKVPMSLLFPALKASIKEPHHTQEEIVISLIDTVLAQDSMRDLLLAETDLSDLLDIIRICAERDDNRLRAKGTKTADSSNAVKTETGEKSQSAFETNSSNRRKYAIATSAVSNLRSSGGQDSSTSPISPTSEFDDKGGPHSDVHDLLPLRPQAFHRDRDDGICQVLAKLDGTSDALDFVSREAVMELFLQLAHRVSDSSGEKMIRYFARERYLIPSNGDWLKLCRHLVAGILQDVSRPRTLRILALHTLRETYETVEQLCPTTTTQQCGALLLDNIESEDDIEVLSELVDFAVSVADRAPVANFLDILQVLKRRLDRQRASSTAPSSFWSPQLMLPNEDKHLGTPSNVICTAFVRLFTRSVTHAAQKTRILYENLRAVAENDKYEPDARLTALKLLFRLRADSRHALIVSHSSEGERIAAELCRTEETAVTPEKGNENNPSDQTKAEDNSSWREQRKTSGSSPHSSLNRHTGRTTTSGGRVSKPIPPLWMYPGSKKGLPEEPPSDPSRVVFSHIDASPYPLSDDVLDMEITLWLELLISLLQRPPDWEIYSYVLVHLGPQLSNQALVRSCVVQLRMLRNVLCEQVRNSSFHEPPPHTLVKKADVAVCLFHILTVIISYHEYFEKSEEDDLVKAFLQGITQWDRTSRWCIHALSVCCFEIPLSVSKSLDSIVQKMSQIITKPATAIHILEFLTSIARLPELYKNFREDEFKLVFGVCFRYLQHIRDLRDRASNNISSQSGPRTLWHSGPSRDFTASPPNTAKPKSVEDDLPGYLYSLAYHVITFWFMALKMEDRAKQIPWITRNLYYTDSTGRQVMEEQGQVIMDIMNMVAYTDRDQTVRNEDFAKLGDGEIWKKTWIVGNRFVTIETAARTGVSLITTRRPCGTRYLTIQPLLTSPPRHQVPITVGLASEAYYSDSYVGILPDDIFQTFYSTFDLVNPSIPLPDDDMTRRAIASFDRIATVDSYKVGVIYIGEGQTDEREIFMNDIGSPAFTAFVADLGTLCRLKDAKFNTGGLDTREDMDGEFTYCWRDRCIELVFHIPTMMPTNRDDEMTYPNKKRHIGNDFVNIIYNDSGLPYNFDTFPSAFNYVHIVISPESRASFVDRRLDADPDGRNRYYKVQAISRPGFPDISPAVETKILQGKYLAAYCRLIAINACVFSNVWFIRGGGESTSSWRNRLREIKRLRDRYGGGVGGDQVHTVASPVVASPASSQILSSPPSRDQNGVSPFQRISIATHITDGTSRSSISSSSHDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM58</accession>
    <name>A0A9W9CM58_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Polyadenylate-binding protein</fullName>
            <shortName evidence="14">PABP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">PAB1</name>
        <name evidence="18" type="ORF">N0V83_004405</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Binds the poly(A) tail of mRNA. Appears to be an important mediator of the multiple roles of the poly(A) tail in mRNA biogenesis, stability and translation. In the nucleus, involved in both mRNA cleavage and polyadenylation. Is also required for efficient mRNA export to the cytoplasm. Acts in concert with a poly(A)-specific nuclease (PAN) to affect poly(A) tail shortening, which may occur concomitantly with either nucleocytoplasmic mRNA transport or translational initiation. In the cytoplasm, stimulates translation initiation and regulates mRNA decay through translation termination-coupled poly(A) shortening, probably mediated by PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM58"/>
    <dbReference type="OrthoDB" id="19742at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12378">
        <property type="entry name" value="RRM1_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12380">
        <property type="entry name" value="RRM3_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12381">
        <property type="entry name" value="RRM4_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1900.10:FF:000004">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000355">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000384">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.10">
        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036053">
        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006515">
        <property type="entry name" value="PABP_1234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034364">
        <property type="entry name" value="PABP_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01628">
        <property type="entry name" value="PABP-1234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 13 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="47"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="135"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="228"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="331"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="17">
        <location>
            <begin position="670"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="362"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="636"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="746"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="362"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="414"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="636"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4371189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="767" mass="83490" checksum="DC1A274C31B50961" modified="2023-11-08" version="1">MSEVANSTSPVQDGADANGTQINTDVAAAAGDAPTPTSAAPAHQNSASLYVGELDPSVTEAMLFELFSSIGQVASIRVCRDAVTRRSLGYAYVNYNSSEDGEKALEELNYTVIKGKPCRIMWSQRDPALRKTGQGNVFIKNLDHAIDNKALHDTFAAFGNILSCKVAQDELGNSKGYGFVHYETAEAANNAIKHVNGMLLNEKKVFVGHHIPKKERMSKFEEMKANFTNIYVKNIDVDVTDDEFRDLFEKHGDITSASIARDDQGKSRGFGFVNYIKHEAASVAVDTLNDTDFKGQKLYVGRAQKKHEREEELRKQYEAARLEKQSKYQGVNLYIKNLNDDVDDEKLRDMFTPFGTITSAKVMRDAMPAERSETPSDEKKEDKEEPTEEPKDEPKDEPKDEPKDEPKEAEDEAKDGSSDEKKEDTKAGDKVTIKGEKKILGKSKGFGFVCFSNPDEATKAVTEMNQKMIEGKPLYVALAQRKDVRKNQLEATIQARNQLRMQQQQQQQFGGIPQMFIAPGQQPMMFPPGGRGQMPFPAGLPGQQGGRGAGFPGGMPGQQGGRGGPNAQQMPPMYMPPGMAPGAFPPYMNPQYLQLAQAAQQAMGGRGGRGGVPMAPMPGMPQGQMAGVPGMRGGQGFPPQGAGRGGMPQGRPGQPPMPSFPQGGRAGAGPAGVDMSALNSAPPGQQKQMLGEALYPKIHEIQPELAGKITGMLLEMDNSELINLTSDEAALRAKVDEAMNVYDEYVKNKEGETEKEAPKEESKEDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM73</accession>
    <name>A0A9W9CM73_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Delta 8-(E)-sphingolipid desaturase</fullName>
            <ecNumber evidence="5">1.14.19.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">N0V83_005389</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.18" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM73"/>
    <dbReference type="OrthoDB" id="260091at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016717">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03506">
        <property type="entry name" value="Delta6-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353:SF30">
        <property type="entry name" value="DELTA 8-(E)-SPHINGOLIPID DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353">
        <property type="entry name" value="FATTY ACID DESATURASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015921">
        <property type="entry name" value="FA_sphinglp_des"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="8 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="232"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="426"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="17">
        <location>
            <begin position="4"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4369627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="562" mass="64652" checksum="62726699A8B158CA" modified="2023-11-08" version="1">MSRHRIVSPAEVKRLIAQNQPIVIHEGYVLHLGEWIDKHPGGRLAILHMVGRDATDEINIYHTNKALLMMTKHRIGRVQLPWANLEPPIRSPDYYKNLKLGEKSGIVAHADGARARRSVDLGSAAAIISKKLMPANSCVRRTSITTDPEKLPLLESEAPTIDTAEDKRRHREQFAIEEEEREIAEGIRDNPSLDVDTQRAVIEDYRTLHQQFKEEGLYQCRYSEYGKESIRYGLLFAGFAYLLYCKWYLSSAVFLGLFWQQIMFTAHDAGHRGISGNFVIDTVIGAFIADFCCGLSIGWWKSSHNVHHLITNMPEHDPDIQNIPLFSTSPTYMKSILSSFYNFQFVWDSACDIMVPYQKYLYYPIMALARFNLYFLGWLHLCSPRAAQLGAAWWTRPLEIVFMCCYWYIFGYLLVWVTLPTWPIRIGFVLMSHLVTMILHIQITLSHWGMPTSDLGPTESFPQRQLRTTMDVDCPAWLDWVHGGLQFQAVHHLFPRVPRHNLRRGQELVREFSAKTGVKYHCYGFVEGNKVVLSRLEQISQMVKMMVDCQKAMAETGESGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMG4</accession>
    <name>A0A9W9CMG4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein transport protein sec16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SEC16</name>
        <name evidence="14" type="ORF">N0V83_005661</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Involved in the initiation of assembly of the COPII coat required for the formation of transport vesicles from the endoplasmic reticulum (ER) and the selection of cargo molecules. Also involved in autophagy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SEC16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="8918678at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09233">
        <property type="entry name" value="ACE1-Sec16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1030:FF:000008">
        <property type="entry name" value="Protein transport protein sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024340">
        <property type="entry name" value="Sec16_CCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024298">
        <property type="entry name" value="Sec16_Sec23-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13402">
        <property type="entry name" value="RGPR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13402:SF6">
        <property type="entry name" value="SECRETORY 16, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12932">
        <property type="entry name" value="Sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12931">
        <property type="entry name" value="TPR_Sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Sec16 central conserved" evidence="13">
        <location>
            <begin position="900"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="domain" description="Sec16 Sec23-binding" evidence="12">
        <location>
            <begin position="1078"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1381"/>
            <end position="1412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1424"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1614"/>
            <end position="1835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="52"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="91"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="109"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="194"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="280"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="299"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="330"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="363"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="381"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="411"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="512"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="536"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="627"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="702"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="723"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="788"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="806"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="816"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1402"/>
            <end position="1412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1482"/>
            <end position="1507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1568"/>
            <end position="1579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1621"/>
            <end position="1647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1715"/>
            <end position="1724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF12932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4369897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1835" mass="195427" checksum="C76910164F830DAE" modified="2023-11-08" version="1">MDNDAGPDFSYAYGGAPATTSAPSWNPALRHEKEGEPAASAQPPAKPRPESSSEDEEDDEDEDEDEDEDEESEDAEDNEDEDEESDEDESPLAPAVSTAPTPASSGTYFEKHKADGDSLARSDSAKGPQNTPKPVEAQIQERRFSGTNDDALARATQALNIKDQTTAPAKRDSSGEESDEESEEESSEEEEAASDERPADNYEHQGESTALEEAIGESVKVPLVADTEATTHDWGHSDDQFILDGQPQQPPLETPHPHAGGTTIGDEVIGNTKVGGNAGEDIDWGNTEEQEDFFGGIASQPSESAQPNDQGSGAHGAVSEAQPATKNEWDLDLDLDDEFLPDPEDAPVFDLSDDEGFLEDEPSAPAQQAQPAVSTSSSTSRYALQTAQGGPASPQTANLYSPQNPQFANLPQQTQQRPPAAPAYNGYGQNLSYLQQQNQQEPTRPAISSAQSFADKSKGGYASPYDLPDDIVTTRKRAAPRASIPASQPTPPPPRTSSMSSSAGPPRPIPSSGMSAASLSPPSSGHSMQAQMTGLPPSTSSKPAPPTKSSSSEFFAELPITSKPKPSGRYTPQHNAPSQFPAHHAPPQLTPKERTASWSSLRNEVLADSATGPPPLRQPEQLPMFPNQPSVPTRTNSLPVPHPAPGPTSSRYSPAPSPSVPSANARYSPAPPTANARYSPAPPSGFQGQTHTRYASEPPNGLNRTPSQPFAPRTSSPLAFHSVPQQQEQPAVVPETAFQQHPGHHVSQSADGMPRPPFRSPLEGVSELEEQGTTMGPGPPMGGRSETPPLQSTPSSTVGSPRKRGTYTSQYQPSNAAAPSRSLSQSPSASLKQPVRSNSTNEQLGAAYGLMSAPSAYATQPSRSSMTNVIPHRRQASLNYDAIIPEDERAADPLQRWRGHPIFTWGLGGTVVTSLPKQIPRYGGGASAPMMKCSPGEVRIRNVKEIFPLPESIATFPGPLKSKGKKKDVIGWLGRRIDALETELKEPGLEHSLTSDDLKRFEDKTLLWRLLQILVENDGKLEGDVTGAAVKKLFSLDAADAADADTSFSAAADIVGRSRSNTSNLQAEPVDPRAIEDLQNMLTKGDREKAVWHAVDQRLWGHAMLLSSTLNKDIWKQVVQEFVRKEVKKVGRNNQALAVLYEVFAGNHEDCIDELVPASARAGFQMVSADGAGPAQNALQGLDKWRETVALILNNRSEGDASALLSLGRLLSQYGRVEAAHVCFIFARSVLLLSGVDDVQADVVLISADHRNNPLELGIDLEPILLTEVYEFALSLSAQNGSHIIPHLQNYKLAHAYQLAEYGHRTDAQAYCDAIAAAMKATTRLSPYYNASFIASLDDLSKRLSQSPKDGSSSWISKPSIDKVGSSLLSKFNSFIAGDDEDAASNPSTGTEVGPFAKIAGNSPSITPSQSSADLYSAYSGYGAPSQPPAPTNSRYAPSNAYAPRTSSEQQRLRYEPQGRLSMESADGSAMRAVSDSYMPMTPTTGLYSPTQSQLSPPRLPKSQSYSPLRAEHNMSQPSYGSPYLPTPPVEESNSAPTFGGYQAPQTSFDEPPPSAENQQPYGGYEPPSNTFEETSYQPYNPDEEDKPGAAQSRKKSFMDDDEDDLVAKAAALKISGGGKSEVDKKADEAFRKAAEADAQRDKESAAAKKGGWFGGWFKKDPNAAPGPIKAKLGEENSFVYDPELKKWVNKKGGSTEAGKPTATPPPPRGGPPGGRSASSSVVPPMGPPQGGLMRPPTSNPGPQRSSSMPPPPPMALPGSRASTPGVPSDTEGSRPPTLVRPSFGAPSGPPSRPGTGMSNASSIDDLLGAPQARKGAGAKKKKGGRYVDVMAQGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMJ0</accession>
    <name>A0A9W9CMJ0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005736</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMJ0"/>
    <dbReference type="OrthoDB" id="429143at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF279">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="2">
        <location>
            <begin position="52"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="330" mass="36918" checksum="6F2BBBB874380867" modified="2023-11-08" version="1">MSTEPRLKEEQQQEQQKDAIFPVPNPVPSYWLSEPHRYSNLRSTPSLPSTCDVAIIGSGMAGILTAYNILETSPQNNTPKIVILEARQLCSGATARNGGHAKVKTATLASLSPSLRTAFQNYVLDVIDRLHAIVDEEHLDCEFELRRSFDVFCDAQEAAEMKSTYDEARRNGEAWTKRITWVDERYVEQVTSIRGAKGAFSVPAASFWPYKFVTGLLERLVDRYSAEQLNVQTNTLVKAVTEEGEEEGEEEGEEEGEEEGEEEGEEEGEEEGKKKGKKKGKKKGKKKGRRGSSRGKVQCPLDRSRRGSRGESRVRNERVDSGVSWGVSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMX6</accession>
    <name>A0A9W9CMX6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004437</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 88 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMX6"/>
    <dbReference type="OrthoDB" id="2317065at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052757">
        <property type="term" value="F:chondroitin hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052369">
        <property type="entry name" value="UG_Glycosaminoglycan_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36845">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G05090)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36845:SF1">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G05090)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="337" mass="38136" checksum="A4F7631E8F2B57D9" modified="2023-11-08" version="1">MGFIVQPALQKDWELTGNKESLQSVVNAAYALASRYDERVKAIRSWDVAINDRYSITNMETNFLVIIDSMCNLDLLYYVGYTQDDQKLIDIATAHADAIIHEILRPDFSSYHLVNFDPRTGLPQAKMTNQGWKDDSTWSRGQAWAIMGFAQTYSWTKDTKYLETAIRCAEYFLRRCKEGEGKWHHHMIPLWDFDAPQEDASEPLRDVSAGVVAANGLLVIHQSLQSLPAATAAHISSSTNFLDIALQIVSETLDLSYDQDLAAFEVSAKRLVNGNDVNGGHGTNEIQVKESGFEGLLRNSTTNWNEHAHRKYKDHGLVYADYYFLEFGNKLLRMGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNE7</accession>
    <name>A0A9W9CNE7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNE7"/>
    <dbReference type="OrthoDB" id="3664249at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="202" mass="22559" checksum="6E818E7A3ECD6099" modified="2023-11-08" version="1">MSNSAPLTPRSSPGSTPSAHVAQTCPLEAFTRIAAFISGASFDLLMGVLSVKTKDQLQAKLAHVNPSTVWIQPSISLTAKCQLIHNWLQQYGRRVAASKDVIPLALILLIRFTVEAHNKDEQLEFDEGVWTVVAIKTAYGNSSDEWLALETWEERTGVPKATIKKAELRFLEILDWRTWIGEKVFTDLGQRMAILWDIALKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNM7</accession>
    <name>A0A9W9CNM7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cyclic pyranopterin monophosphate synthase</fullName>
            <ecNumber evidence="3">4.6.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_003414</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:49580"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.6.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.17" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNM7"/>
    <dbReference type="OrthoDB" id="429626at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01420">
        <property type="entry name" value="MoaC_PE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.640">
        <property type="entry name" value="Molybdopterin cofactor biosynthesis C (MoaC) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023045">
        <property type="entry name" value="MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047594">
        <property type="entry name" value="MoaC_bact/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036522">
        <property type="entry name" value="MoaC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050105">
        <property type="entry name" value="MoCo_biosynth_MoaA/MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002820">
        <property type="entry name" value="Mopterin_CF_biosynth-C_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00581">
        <property type="entry name" value="moaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006870">
        <property type="entry name" value="PRK09364.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960:SF0">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01967">
        <property type="entry name" value="MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55040">
        <property type="entry name" value="Molybdenum cofactor biosynthesis protein C, MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin cofactor biosynthesis C (MoaC)" evidence="8">
        <location>
            <begin position="561"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="282"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="458"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="185"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="283"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="504"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4373123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="733" mass="80866" checksum="29C315D477918E02" modified="2023-11-08" version="1">MFADKHSDQMRLWESSSRIHAEVVEPMMDRDSQQEGGTIANVADTLRDGRNMKRALRQISSPRVTKSMFTDIHGQIRQLEKRIAHDKKYDPATDPGSWQDFKKSTQTEMKLGNIKMSAASRRVLWQDWKRNSAHSDKDPTELDSKRQALRDEHEKKIRNIQAILFKYKMHLAAAEGVKDERREELDHVRKQQREVRAKKQELLAQLKELKEEGKAVRQVRATRQPTPTPTIDRQARAESFDVGDILDDAVEKIERTARKLRRQGFAPIDDVEISSVAAKLSAKNRTIQSPTSTAADADSESDTSVRRPSDDLHLQVPASGLIHLDSNLTVNLEAGVADLQSQTLQMQERLKSSYPRLDTLPYDVSTSKSEKTLKTWLKILVSRWQTRFDDSGDGSGKGEADNINEHVKSVMDHMVRDHDLSNEAAERMAKRWAEVFETRSHKSEHAAAPLDWDELDAGGMGFLKDDSASQVSQPSTKEEPRSWQNHRPLAHVKAEAPSFDSLTRRLYSTSSRPPLDLNMSPSSTPAAQSAKSTTATSTAQQPPSQKPPSLPHLTSSGSAHMVSVSAKPHTIRTAIAVGTVSFSNPTPLSLIRTNSLKKGDVLSVSRIAGIMAAKKCPDLIPLCHPISLTHVGVELYVLDGAGAIEGEGCMAFGGVAVEAKVQCTGPTGVEMEALTVVMGAALSVVDMCKAVDKFQRIGDVRVVLKEGARVGFGGRRVGVFERGAGGGWRGKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNP6</accession>
    <name>A0A9W9CNP6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_003659</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Secreted aspartic endopeptidase that allows assimilation of proteinaceous substrates. The scissile peptide bond is attacked by a nucleophilic water molecule activated by two aspartic residues in the active site. Shows a broad primary substrate specificity. Favors hydrophobic residues at the P1 and P1' positions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNP6"/>
    <dbReference type="OrthoDB" id="2747330at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06097">
        <property type="entry name" value="Aspergillopepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000024">
        <property type="entry name" value="Endothiapepsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000026">
        <property type="entry name" value="Endothiapepsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034163">
        <property type="entry name" value="Aspergillopepsin-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF2">
        <property type="entry name" value="ASPERGILLOPEPSIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040913434" description="Peptidase A1 domain-containing protein" evidence="9">
        <location>
            <begin position="20"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="10">
        <location>
            <begin position="98"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4373364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="409" mass="42939" checksum="27329727F3BD5A75" modified="2023-11-08" version="1">MPSFAHLTAALAFTSTALATPVQINKREAFSVQQVPHGTYLKNGPQQKVKALRKFGKTVPQSLLEAAENRANVFTATAAGAVNGTDPAVPSDEYDSSYLSPVTVGSKTVQLDFDTGSADLWVFSSLQASSQLAGHDYYQADASKLKSGYTWKISYGDGSGAAGKVYADKVVVGAVTATTQAVEAATSVSAAFSQDQNTDGLLGLAFSTINTVKPTPQTTFFETVKSQLAAPLFAVDLKYHAAGTYDFGYIDKSKYTGTITYINADNSQGFWGFSATAYSVGTTTTKATITGILDTGTTLIYTDAAIVKAYYAKVSGAKVDNTQGGYVFPCSATLPNFAITVGGVSQVVPGKYINYAPLSTGSSTCFGGIQTDDGIGFSIFGDIFLKSKYIVHEYASSTPRLGFAQQAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNY6</accession>
    <name>A0A9W9CNY6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ENV7</name>
        <name evidence="13" type="ORF">N0V83_004292</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNY6"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032889">
        <property type="term" value="P:regulation of vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006624">
        <property type="term" value="P:vacuolar protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000550">
        <property type="entry name" value="Serine/threonine kinase 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000374">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052239">
        <property type="entry name" value="Ser/Thr-specific_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45998">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45998:SF2">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="35"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="164"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4372518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="392" mass="43570" checksum="05FD4F4913FC8895" modified="2023-11-08" version="1">MGSLGTYFLDVVYSFTNCMVCFPSSPQLKINSRSFKILRLLGEGGFSYVYLVQDNSNQQLLALKKIRCPFGQESVSLALKEVEAYTLFSPHPNIIHSIDYSVETDKSDPSAKTVYILLPYYRRGNLQDMINANLVNHTKFPERRLMVLFLGVCKALKAMHQYKVKGGPGGARSERKAKKVRNDAARADAQAAESMEMRPSRRHREQDDDDEQEAEGLLEDSNVTMAQEGIAPGGERAYAHRDIKPGNIMIDDDGQTPILMDLGSLSPSPTPITSRSLALQVQDTAAEHSTMPYRAPELFDVKTGSVIDTKVDIWSLGCTLYACLVGKSPFEARSDETGGSLSICVLGGDWRFPDEAVQRGDRGSNRRRWVGLGLGVRNRRLRKVSRRLLGCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP59</accession>
    <name>A0A9W9CP59_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methylated-DNA--protein-cysteine methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.63</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_002947</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MGMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.63" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP59"/>
    <dbReference type="OrthoDB" id="1907495at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10815">
        <property type="entry name" value="METHYLATED-DNA--PROTEIN-CYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10815:SF13">
        <property type="entry name" value="METHYLATED-DNA--PROTEIN-CYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="Methylated DNA-protein cysteine methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding" evidence="5">
        <location>
            <begin position="10"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4374206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="120" mass="13202" checksum="AC86657F53591DFB" modified="2023-11-08" version="1">MSKQHKVTLYQERVYAALQQIPEGRITTYAALSKALDSSPRAVGGALRVNPFAPEIPCHRCIASTGFVGGYKGDWEKAPSGQNQESKLQLLKDEGVLFDAGGFLIDKSQLWDEFDSSKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPG6</accession>
    <name>A0A9W9CPG6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translationally-controlled tumor protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_003629</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCTP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPG6"/>
    <dbReference type="OrthoDB" id="10248936at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.10:FF:000002">
        <property type="entry name" value="Translationally-controlled tumor protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.10">
        <property type="entry name" value="Metal Binding Protein, Guanine Nucleotide Exchange Factor, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011323">
        <property type="entry name" value="Mss4/transl-control_tumour"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034737">
        <property type="entry name" value="TCTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018103">
        <property type="entry name" value="Translation_control_tumour_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018105">
        <property type="entry name" value="Translational_control_tumour_p"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11991">
        <property type="entry name" value="TRANSLATIONALLY CONTROLLED TUMOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11991:SF0">
        <property type="entry name" value="TRANSLATIONALLY-CONTROLLED TUMOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00838">
        <property type="entry name" value="TCTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01653">
        <property type="entry name" value="TCTPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01003">
        <property type="entry name" value="TCTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51797">
        <property type="entry name" value="TCTP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TCTP" evidence="3">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4373335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="169" mass="18798" checksum="87E377C7E5EA7285" modified="2023-11-08" version="1">MIIFKDLLTGDEIISDSYDLKEVDGVVYEADCKKISVGGETFDTGGNASAEEAEEGVEDAAEQKIDVVYSFRLNETGFDKKGYLTYLKGYMKAVKEGLKSKGADEATIKDFETKASGYAKKIIGNFKDYEFFTGESMNPDGMIVLLNYREDGVTPYVTVWKHGLEEMKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ34</accession>
    <name>A0A9W9CQ34_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ34"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="190" mass="21069" checksum="68F4099E23CE9225" modified="2023-11-08" version="1">MSGNTPSSELGSMNTLPSETEPLLHNRASDPEDKTGSRDDESVSMAETSTKNDFDSDFDSDDDEAEIKKKREQRLKETGGWLGYLRDFGLFLPYLIPRNDIKVQLCIVVSLLALAGQRVLNILVAHQLGLIADKILDRTPPYKELAVWFVLSAMNDNSGLGMIEQIATVPVKQFSERQVTNAAFSHVLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQD7</accession>
    <name>A0A9W9CQD7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YMC020W-like alpha/beta hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001397</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQD7"/>
    <dbReference type="OrthoDB" id="5598028at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058934">
        <property type="entry name" value="YMC020W-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058933">
        <property type="entry name" value="YMC020W-like_ab_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47349:SF1">
        <property type="entry name" value="AER328WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47349">
        <property type="entry name" value="CHROMOSOME 8, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26147">
        <property type="entry name" value="AB_HYDROLASE_YMC0-YMC35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YMC020W-like alpha/beta hydrolase" evidence="2">
        <location>
            <begin position="519"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="312"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="461"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="473"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4376116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="945" mass="101557" checksum="5DCD391BAC0F29CA" modified="2023-11-08" version="1">MNPRKKTKPNPQAPAEDDNSSTGPQVPPDADDATTNTTSSTPSGSRVLQNESSNKSIAESTTPSEATTRSSTSGAHTPKGKKWLSGTGSWRSKAPAIVKTAKESIGVTGGATAELPGEDAKKPRDDSPKKFLTKRKSSKGDAIPAAMTKVNVSSDGLVDEPQNPKADASPNEAQEPPTPKVDEPPLPPEPPKAQADATANTWGWRSWWSRPDGYPEANKAKSEAEGTIQDAQSTPLPGPTPSEEPDAKTKGLGSGPVVVPSDQNYDAEVEDASAQPVSGQGTDVKDAQPATDKNSTRATSGSWFWLWSSAQNAQANASVAPSSTEDTPALEPDQASEAVPQIGPEVSPETQPSQPVQEAVQEETPTPPAAAQSATSGSAKPSAWAFWYRGKPGEESKATEGGAQKHVGEVAVFDTPSQSNPEAAQFNEQAEPKPDISQQPQQPKEEPLAKRSFRSLRGNPKTKSGSKDTPTNTPGSSKTATPTKTSPVLEPTRPETAPAAQPAPKQVSKAKQEPTNLVLPEFNTTYRLVQHPSFWQQVRQYFLGNEPATPHLHINPAPPRIKKAVAIGIHGFFPAPIIQKVLGQPTGTSIRFANAAAAAIKDWSAARGYEPEIEQIALEGEGFVAERVNSLWKLLLNWIEHIKAADFILVACHSQGVPVAMMLVAKLIQFGCVNTTRIGVCAMAGVNMGPFIEYKTKYFGPTAAELFEFSNPKSLVSQMYLAALDEVLRFGVRVLYVGSIDDQLVSLESSTFSTLSHPYIYRAVFVDGRIHAPDFLTHLVGFTLKLRNLGLPDHGLIRELSPALAGSLYGGEGHSRVYEDPAVYSLAVQHALETTSLPIPPQQRPSTSGSFQGINIPIIGEKASGSLREDVFKLRVKDYEQSASTANQNPFFLPWAMRGLLEEDFVKKELGKEVEDLLGMFEAWKPTGKQLKDVKFRLEGVRSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQK4</accession>
    <name>A0A9W9CQK4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_002058</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-binding monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQK4"/>
    <dbReference type="OrthoDB" id="74360at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051209">
        <property type="entry name" value="FAD-bind_Monooxygenase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42877:SF1">
        <property type="entry name" value="FAD-BINDING MONOOXYGENASE STCW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42877">
        <property type="entry name" value="L-ORNITHINE N(5)-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4374979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="506" mass="57186" checksum="3EE3C7764B783EB2" modified="2023-11-08" version="1">MKSYASSAEIRSYLKAVTCHYEIDRYISLRSAVTRTVWSETESKWTVSVQNQGDFDCEILVNACGILNNVQYPRVKDLDLFQGPLLHTAAWDDNVDLSGKRVAIIGAGASAIQTLPAIAETVERCDVYIRTPSWISPPFGTDKPGNFTYTEEEKQRFRDDPDFSLSTRKAMETSFNNLYSASVKGSEEQVALRNALEKSMKALIRDPELHDKLIPKFEVGCRRVNPGERYLTALQLPNVHPVFEPIDSVTPEGVVCGGQLREVDAIITATGFDTSFRPRFPIIGRNGRDLRDLWRDDPVGYFGLAVSGFPNYLVFLGPNTPISNGSLMGTLEATVEYFIRLFKKFMHEKAVSFDIREEVQADFDAHTQDTMKQMVWTGPCRSWFKSEDGKVRALWPGSSLHYREVLESNRWEDFKWGYRGNRFAHWGLGRATVEFESEKTRDLAYYFEKHKPLPLEAYYLAAIGNDARVAIGDSFRKSGEGGEEGKVVLEDDDEDTLVNTPEVVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQM7</accession>
    <name>A0A9W9CQM7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Iron-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ISU1</name>
        <name evidence="9" type="ORF">N0V83_002271</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; iron-sulfur cluster biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NifU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQM7"/>
    <dbReference type="OrthoDB" id="1925777at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06664">
        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1010.10:FF:000005">
        <property type="entry name" value="Iron-sulfur cluster assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY ENZYME NIFU HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82649">
        <property type="entry name" value="SufE/NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIF system FeS cluster assembly NifU N-terminal" evidence="8">
        <location>
            <begin position="5"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4375187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="134" mass="14091" checksum="44E5DFD507015A90" modified="2023-11-08" version="1">MSKADTDVGTGLVGAPACGDVMKLQIRVDPNNNTISDVKFKTFGCGSAIASSSYLTELVRGMTLEDASRIKNTEIAKELCLPPVKLHCSMLAEDAIKSAISNYYTKNPNARATDLGGKSAPLPKVEIETVSAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQM9</accession>
    <name>A0A9W9CQM9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQM9"/>
    <dbReference type="OrthoDB" id="3701248at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="155" mass="17746" checksum="3DCECB38D07F0B09" modified="2023-11-08" version="1">MSYSSSNSSMTSLSSTDEKISMLLLPATDLLVRDFGWPSSSARYRGDHTPDSNPDWEHDPLDSAEAKEEAYKKMEKSYAIYMGNLEGARAVPKKEKKPVWARFLPRDGQTRLETRTYIDSFLKGGLAYMIHEWLVQLFGDPERPWRRLVRGQIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRK3</accession>
    <name>A0A9W9CRK3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Mediator of RNA polymerase II transcription subunit 13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Mediator complex subunit 13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SSN2</name>
        <name evidence="16" type="ORF">N0V83_000851</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 11">Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the SRB8-11 complex, which itself associates with the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Mediator complex subunit 13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="103819at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009401">
        <property type="entry name" value="Med13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051139">
        <property type="entry name" value="Mediator_complx_sub13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021643">
        <property type="entry name" value="Mediator_Med13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041285">
        <property type="entry name" value="MID_MedPIWI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48249">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48249:SF3">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06333">
        <property type="entry name" value="Med13_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11597">
        <property type="entry name" value="Med13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18296">
        <property type="entry name" value="MID_MedPIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0010">Activator</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 11" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med13 N-terminal" evidence="14">
        <location>
            <begin position="1"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="MID" evidence="15">
        <location>
            <begin position="1071"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator complex subunit Med13 C-terminal" evidence="13">
        <location>
            <begin position="1264"/>
            <end position="1542"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator complex subunit Med13 C-terminal" evidence="13">
        <location>
            <begin position="1590"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="631"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="742"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="791"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="902"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1008"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1400"/>
            <end position="1458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1538"/>
            <end position="1597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="437"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="700"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="710"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="797"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="905"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1402"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1437"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1538"/>
            <end position="1583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF06333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF11597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF18296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4378021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1673" mass="180915" checksum="A35F5B3470BFE119" modified="2023-11-08" version="1">MEFLKTCSTNAQAIGDFEAVAYQALSSSRSTTQPSLRLHDGSPSEDIRAIEAELRQGLSLVIQDVSRPWLWLFQPSTVDKAEAGQGAVELPELDGYCLQREQSGFIKATELSRPPLRALNSTPSSGSNISFSTASHKVLLSGGARPSQGSSQSQGTDSSQPPDCFTLYDLFTSSVIALISFHLVRDCSVVALNHRTFVSQPLTTQGNDNAKPAMPPIPHWLTGINVHWISSGTLLVSTFTNRNPEIRCVADVTLPSEQELLVGRCVRVAPNGLLARIASFEDPLDSLTEEVSQRPRKRAKIGSLEQSIEKWKSTVKRWLGWRGYSLPNLEKRSSWVRLRTGQMNQSTVSSPAPSASDRDIIWPRALCFYYFTDWTGNTAPETSTILTSEPADNDLRWYETPDSTGFRDPLDVAQQWFLGKPNRDKILEARRKAKKAEEEAARRKEDRPSLYASSPLNARNGAYGDFQSVSGVYPTPPDGVVPGTAVSYSDTPSVSGGTSNIILAPGGNNPAINLSAPQDNGHSDAQQLPMTSPVFPSGPDNFNTSSGNDDLFEDMEEDGYGGNGVNDADFDFFDGPDEEDVDMVDASALPDDQITTIKDGQVPEPMAISEQVKEEMPDHLAALENALATASEPTDAELPKIEGNQPPATPFLAQKQGDTSMEEEEEVSPQAHRRMSTTKEPTPPLSPGMIAKTLQPSPPNKSTSQNLQDQKLRSQRDSAFDPLIFSRKMSISDAKYHDGLSSVQHDKNINDRQASPAPCRTKSLRDMPLLTKLRYAIGVASANRIPEIPSLARAVSDDSDSSSESSDSSEEDSEEEATTGPEALVGGIILPAKRRLPTEGNATPMSVTSFAESLGSDWHDLQGLQLDEAALAFFEPDTWDWSLVMFPSPSERAAAGARYNMPALSPSNAQMPDTPTSQSDFTPEQPDEKPLILSGKDSIAITQIVTDQIVSATLDILGEDQLLQQDSNYPASPETRWQATIRNIFPNATECNLPTLAAVHDVFPELSAQAKGQQRPPPRKPNEGAAIPGNHLFPLNPPFLRVRRAETHWDLLPPAIAFWEPLGLSPISSPKNIVTFCVYPHSESLRPCLENLLLNLQLAYDSCKLGSHARVETVVEFEGGLVPCRVTTPGTRREAIKVLRETCVQLGKLLAIQHGKIREQQDSKIDAFVIYMVDSFGGPSGLWELGSAFWALFQAYSQGVPGRPDQPQKPDLVLQVIPMKYIASFDVPVILDPSTYINLAREVYDRCPPSAPSGDKTTLSIYKAPAFQLEETLPRTIPFRLISEPAQDLLRENSYMHLGYAISLDGNWITAAWTDSCGKSQAVVSYHLGTRVFAEIAKEIWQTTIEILQSRRVHWRVVIAKAGVVERDEFETWVSLISYPTQLNVFIMLFTVDTDPPFKLTPSMTPTHNPTTGQPSANSPDPASQAGVSPDPAVGLTPAATPSADPTTTTTDPSADPEARLIDVTDESWGVILAHRLHNSNSTNQFNPALISGLLVKRGETFATSNTINHPIPDPQHGPIVVAVNMLWIGAVGSNTRASAATSSPLPSPSAGTGSDGVSPGSSGVTPNPYATAATTGAGSASAPPSPSPATTQQDGGVRAASSLIWAPTVQTRSTAENLLREVLVQYRALGLLAKLRGVRGSRHGSVPWHVAAAKRGVGGLERVVGGGGGSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRL9</accession>
    <name>A0A9W9CRL9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRL9"/>
    <dbReference type="OrthoDB" id="4138492at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF11">
        <property type="entry name" value="AMP-DEPENDENT SYNTHETASE_LIGASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="566" mass="60406" checksum="37104946D49611D5" modified="2023-11-08" version="1">MAGLLEQLDSAISDLLTGWNVYTTLLAVGILGYVGWVIYDTADADTHPLLLARQAQASYVRQPGESAVFRSPETPHGYPLRTGLAVKPAGAPMYSAGKDGDLRDIWRRATGEIPLEKGATSSGTAKIMTVFGKEGISEHKIEAVTKEIAIIGTHLKDQGAKRVAVYLPNSIEFLAVLFAGAFYDFSPVLIPYNQPHATLVDLLAQTGADALVAEAGSFPLADISRGAPALRQIIWTVEKTSRHMDWSEVPEGIGGKLDVSVWHELVQDRSNGTVVLPSDNKKAPSVVFLWQESVGKSAEIVEFTQQNLAAAVGALNTSLPLVHRLNASDTFLPADAFTHSYSLCLTLSALFAHATVIINSVAGPGVDLTLATRSIAPTVAVISAETAAKLHSTTATSITSGPKKLAHYLETRMLTAGRLPTDTFLTKLNAPARANIGTTPGKLRLVFVSERAGLNTPPLSEEDLSDLRIYTKARVVYALTTAKVAGAVAQTNIYDYRRGLTQSSKHSHFGVPLSCVEIKLKDTPRHKTTDEQSAGELVVTGSSVAGGEVNLGVNAVIRDDHTLAYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRN1</accession>
    <name>A0A9W9CRN1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001734</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRN1"/>
    <dbReference type="OrthoDB" id="1911848at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37542">
        <property type="entry name" value="HELO DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37542:SF1">
        <property type="entry name" value="PRION-INHIBITION AND PROPAGATION HELO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="359" mass="39671" checksum="9269ED557990852B" modified="2023-11-08" version="1">MPGATTSPSDIDEGVRTEAIYNNTSVWIEWKHPDPSGPPQYDGGMDPKIHARVRKLAALLSKNNRTVRFRAPFCRGYFIDQDEGRFGLVFEKPASVPPETAPTSLHTLIRAANTPGSTVEVPSLTDRITLMKILAETVERLHAVDWLHKGLRSTNILLFPKFPRKDSGGEDEEEMNYLAPYISGFDYSRPATSDDMTERPLENPAADIYRHPTVQHEGNREASLGGHRENYKPSFDIYSLGIVLLEIAHWKTIDQILSIDLESPRLRPKDTWAVRERLVGGGGGAGGEVVRGKRGGEETFEVRKELSGATVEGVVRACLEGPRAFGLDEGCDEKREVVAAGLQRAFGEGVVRKLASIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRN9</accession>
    <name>A0A9W9CRN9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_000959</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRN9"/>
    <dbReference type="OrthoDB" id="3763672at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008630">
        <property type="entry name" value="Glyco_trans_34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306:SF3">
        <property type="entry name" value="NUCLEOTIDE-DIPHOSPHO-SUGAR TRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05637">
        <property type="entry name" value="Glyco_transf_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4378126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="259" mass="29601" checksum="E480AF360682F3F3" modified="2023-11-08" version="1" fragment="single">QPLSATASIQALWSSIRLPVTAKTYTDADGKQFNLTSKPSWTKPLGKDILIIDIDTRQPTGYNQILNTTKVNWEKLGDGTYMDDNIGLTSSAIFNHYLYSQIHGYDYVFFQSKEIGYQHHTWVKVHAIPEVLKNYKFVVFLDADVTVHHMEVPLEWLFNRWKITPQTSLALPQDVTREGCDTCDSKGNVMLSSGVIVAQNIPHTHKIFDAWKSCPNETLYKGCGEWAYSWAHEQRALSEFLRYDENMNPNQNILVSHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRV5</accession>
    <name>A0A9W9CRV5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000292</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRV5"/>
    <dbReference type="OrthoDB" id="2130169at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051783">
        <property type="entry name" value="NAD(P)-dependent_oxidoreduct"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48079:SF6">
        <property type="entry name" value="NAD(P)-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48079">
        <property type="entry name" value="PROTEIN YEEZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="1">
        <location>
            <begin position="18"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="341" mass="37271" checksum="C7ACEE6A1C0F4C21" modified="2023-11-08" version="1">MPKQHTMSTSRSAFLIGAGYIGLNVLDELLGAGYRVTVLVRRPEQASHLEEAGAKTVIGTLEDLDLLQKQTAEHEVTINTSSSDDLPSVEAILAGVRQRIEAGQATTFVQTGGTGTLEDGAGGMHNKGHVYRDDKPGDIEAIPPTSLHRHVDIPIVQAAKELGDKAKIVILLPPFVYGVQPAHTRHSFAIVYLVKFALKHGHAGHVGDGVNLWSVIHAKDLGRAYTTILKYLDTAPAKEILENPYFFAENGTEITMGDIAREIAQTLHRLGKISAPKVQTFTNDDYQDLFGDFTARGFGCNSRSRAIRLRELGWKPVEKDAWTSWREDDVPSIVAAYEAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSF1</accession>
    <name>A0A9W9CSF1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3419 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000899</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSF1"/>
    <dbReference type="OrthoDB" id="10253390at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021829">
        <property type="entry name" value="DUF3419"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47473">
        <property type="entry name" value="BTA1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47473:SF1">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11899">
        <property type="entry name" value="DUF3419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4378068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="517" mass="57929" checksum="4D4F5E347F24B868" modified="2023-11-08" version="1">MIEDYLEYRFGTVLSINARNNFFNTGIKLPYYIWVGCSKDHGASTQKLAEIDAAATESPYLSALDLQTGATQTDVEVRSKAYESAIVNLQSSLPLPATWYQNHHWRIHYDSSLPKHTRFNDSYIYAFTWEDDVADMRLLKPTSNDVVLAIGSAGDNILAFCLENCRRVHAVDLNPSQNHLLELKVAAFTALGYQDVWKLFGEGKHADFHNLLIQKLSPHLSSEAFQFWLHNGPSVFSKPSSKGLYYSGGSGNALAIAGWLFRILGMSADVKKLCAAQTLNEQREIWQRSIKTVLHSKILTWAILGNEKWLWKALGVPPAQRNVIEVDFKKQQDYAPPAKKDTAAGEDYMAFHEEPSDDALKSPSGNAIYEYVLSTFEPVINTTLLSTSNHYYLLTLLGHYTPSSHPTYLSPKSHLRLSKPNAFSGLRIHTDEISEVIARMRPGTLTIVVVMDSMDWFPPGGSQALKQIKALNRALKVKGRVLLRSAGLEPWYVAVFGECGFRCRRVAVRVPGSCIDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXJ0</accession>
    <name>A0A9W8XXJ0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_010959</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXJ0"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4362018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="185" mass="20863" checksum="A19A7784D72EC424" modified="2023-11-08" version="1">MADARGNKSEIAKNNRVAPIIYEVVYNYYNNFGYHQSIPLNLPKTRLLLLSNEFAAHTISRSQIQSGTILRRANSKINFKGSSPFDPDSTYYQWKSWALSGTPLERDLQECLIFITLALHGLKNSKRPPSKDGRKGVKTFENTVFSAVATEGKRKSDKIGGLELAHRWKKLTYNVKDGVEKVLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXW0</accession>
    <name>A0A9W8XXW0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glucan 1,3-beta-glucosidase 3</fullName>
            <ecNumber evidence="8">3.2.1.58</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">EXG3</name>
        <name evidence="8" type="ORF">N0V83_010762</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.58" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXW0"/>
    <dbReference type="OrthoDB" id="1887033at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046557">
        <property type="term" value="F:glucan endo-1,6-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000100">
        <property type="entry name" value="Glycoside hydrolase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF43">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3 5 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="7">
        <location>
            <begin position="114"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4361821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="549" mass="62111" checksum="3A2122E6F6894B86" modified="2023-11-08" version="1">MRGFLQKAKAELRGINQRDTSTHDPGGHYWVSHHPQAQLPGQVHDYSDSDHANAQIIREPTPLDILRYRYHHSTNLGSIYVLERWLQPSRFPDGAEGSSELAAVTAWVNKIGIESTKQLFEEQWANAVTEEDIQWLVNEARCRTIRLPIGYYDLPGADFTRGTPFEPYARVYEGAWNSIRTLMSRLRTHSIGVLIDLHALPGGANSQEHSGTNSGRAEFWNSSSNRALGVRCCQFLAEDARTGSEIAGIQLANEAMWEAERMYEWYDECISAISAMDASIPVIISDGWNLNQAINYSLQTNSVNINQPKSPVVIDTHYYWAFTDDDKRKSPQQITSEVGTKLTELDGKEGSVIDRGAVQVIVGEYSCVLTEDSWAKADGTPRDELVNQFGQAQSTRYQQRAGGSFFWTYKMDWMPGGEWGFKDQSDKGNIVPPGYATLSMDDRSRLVEKAKYERDGSMREAVQQHVAYWDRIDSNVNRELGKYEHGWTVGFQDALAFFEGRATQGDKIGMLELWVLKRMRESGYRGGLTWLFEQGLRKGIQDLYAAVNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY45</accession>
    <name>A0A9W8XY45_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009998</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY45"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="71" mass="8016" checksum="DF06290612F18301" modified="2023-11-08" version="1">MYHASVPRDERELLLALRNRIIALERALTPFSPVPSSSSDDDGDDGSDIDRADKKEKEAKDKEEEKEEDKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ46</accession>
    <name>A0A9W8XZ46_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010057</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ46"/>
    <dbReference type="OrthoDB" id="3792527at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF73">
        <property type="entry name" value="HET DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G01020)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="877"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="2">
        <location>
            <begin position="195"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1016" mass="114400" checksum="2A13EF36D8B515E1" modified="2023-11-08" version="1">MENSLGVQSGLAVGIIGGSLSGYIYVSFEIQRYAATQIDGHSRLQQYSVGLLLGCLQLYLNESAFALLPYHLGLAGFNSRYTWFIYPVVYQVAALVAWWTGYLPIFDLRACGQLCLLWMDNINGRIRIRDLDASPCANSKSDSLPKNNKARQKYSYKRLTGESSIRIARIRPSLSLEGPIRCEVVEETLDSPPPYIALSYAWDGHEGKVSVICNGSNLSVTPNCARALRQIRELHGRYHAVDVWVDAICIDQGSDPDALSERSQQVQIVGEVYKKATHVIAWLGDHQARSQEICDYLVQVSKACLNEENETQAWAAARNVALEMTRNWMGFTYNFRNFFRRSWFQRLWPVQEATLPLPGKVTLLCGDSLVPFDYVRVGWQILTDIGLVAGSLNLDQAVALQFYISDALALKRSEQMTTGVRRFGRPFITDLSSFSLTSVMRATRFKSCSVAKDKFFALYGVFKELGMIHDSADVDAIRYAQLSDNDVFFVVFMLCFAKDRDLNIIKSARSHERYLTHDDFWATQLDPYDTSGNTVLSLGVRLRSWITGHIKEEAPQWRTALPSFVPDWMQWTPGDMDDSDIALFRSYSATQLWNMRPVPTCGIEKRPRVTLLNPQHGPKLLQKRRSPSDPSKLLPSGLLVRAKVVGVIRSTGAVDSVAVLWDIVASSFNFYGYRVGWHAAVNDLETPKIPFFRLVPEAVVAAIIESVRNFVLNSTVAPVLLCLRSIYRECNLPTLVTYARSCILVWSLWPHFMRWICSSYQSATASCSAVGSSWTVTSYRSYPARIVLMSTVYHYYQSRFFGSRFFGREFFYRLFRKNAHFAVISGTCLLWTFRDATFRAMYGEAVADPTSRFMTIVQIGLMSQGISLAGTQLEDNIVILLVRMVMAINTIFLTIGLALRTRLRYVTMYVLTTLMVHASSCAMGTLKEVIGSNSFKRPGTMSSGLTFFTTDTWLTGNTGGAVQLFDVVTVIDGVEDPMILRRRGKLFEIVAVAYVGNKTRKELIDMAGPWQANILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZV4</accession>
    <name>A0A9W8XZV4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_009558</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZV4"/>
    <dbReference type="OrthoDB" id="1735038at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070008">
        <property type="term" value="F:serine-type exopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000636">
        <property type="entry name" value="Serine peptidase, family S28, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008758">
        <property type="entry name" value="Peptidase_S28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010:SF109">
        <property type="entry name" value="PEPTIDASE, FAMILY S28, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G03790)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010">
        <property type="entry name" value="PROTEASE S28 PRO-X CARBOXYPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05577">
        <property type="entry name" value="Peptidase_S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040976064" evidence="6">
        <location>
            <begin position="21"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4364103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="590" mass="66037" checksum="0F0C0B7C7870CA60" modified="2023-11-08" version="1">MRTSTTIAALALALLHPTTAMNNAMQRKFGQLAEIGLNPDGSLMDLPSADFENPTIDVASLKSDKMATTSSDVSITAVETIVPEYVELPIDNFAKDKNEAYSYQGTFFNRYWVAQSAYKPGGPVFIYDVGEANAEPNALFRLQNETSFFKQIVDSYNGIGIVWEHRFYGNSTPEPIDVNTPPEAFEFLNTEQSLADVDRFAKQFSRTNINYTLTPDKTPWVFVGGSYPAMRAAFMRNMYPNTIYASYASSAPVQASVDQSFYFEPVWRGMNKYGFGNCSRDIQAATRYMDKIMDTDRAAAARLKVQFLGLGAANNSHATFADALTTIFATWQSYGVEGGQMGLRRLCDWIETDPDTQKLAPAEGWAKSKGANFTVARWASYPWFASNVNTYMETNCSGRANISGDCDLNRKFTDPSMISWTWQYCTQWGYFQSANLGPNQLVSKYNSLLHQHDICHRQFPSAPRSLFPDWPLTDRTNAIFGGWSIRPSNTYWSNGEFDPWRTLSPASAEPFAPHPVHIFTDPVPQCGQGQSRRELFGYVIPDAQHCYDFRTTFAGGEVSRGYFRSALTKWLGCFKPKKGGIPGQGKPWTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZZ5</accession>
    <name>A0A9W8XZZ5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="16">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">DNF3</name>
        <name evidence="20" type="ORF">N0V83_010639</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 16">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="16"/>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000090">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000172">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF174">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE DNF3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 15 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="600"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="642"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1453"/>
            <end position="1474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1486"/>
            <end position="1503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1515"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1561"/>
            <end position="1581"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="18">
        <location>
            <begin position="328"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="19">
        <location>
            <begin position="1339"/>
            <end position="1589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="418"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="746"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="981"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1616"/>
            <end position="1656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="220"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="429"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="746"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="988"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="1626"/>
            <end position="1635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1636"/>
            <end position="1646"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="13">
        <location>
            <position position="712"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="712"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="712"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="713"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="714"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="714"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="866"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="914"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="937"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="1118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="1198"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="1199"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="1200"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="1286"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="1292"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="1313"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="1316"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="1317"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="1317"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ4362545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1656" mass="183179" checksum="54B7118A831E832B" modified="2023-11-08" version="1">MSGAGGPSGDARSFDSAGEGIRRRHSASHDEDELSPATRSAAESATSPYTVESPQRSNINPDAAPVSRTSAIENSARPSISRLSSDTAPRVRFSTDIDRHELGHKSSLAGERPTAEETAYVEGSAVPKKRPGIPDLTIETHEVSPDTEGNSSRQGPGELPPRISPLSPRTPSNRSPLSPTSRTRGYSLRSSLFRKNVHDTTQPLGSNIELEEGVGSSHGRIHESGGYEASAKGDAKIVVTPEHVEYTSQGSDLSKSKLGKGPIALPHYDTWLHKKGPNKKAIIGKKIRESAEQARKFIFRIKDIPPSKDGRHIQLDVARKECLIDERTSQPYTSNWIRSTRYSAWNFVPRQLVAQFSKLANFYFLVISILQMIPGLSTTGQFTTIVPLLFFVTLSIAKEGYDDLRRYRLDKAENNREARVLRASPSSVSRQESADGGSSSSPVSETLIWESIKWQDINVGDVIKLDRDDAAPADLVLLHTSGGNSVAYVETMALDGETNLKSKQTTASISKTIIDQEDVLRTDAEFVIEDPNRDLYSFEGRVMVDGKQAPLTLNEIIFRGSILRNTPDAIGMVIYSGEECRIRMNANKNPRIKAPALQGLVNRIVILIVVFVLLLAIFNAVAYKIWQRHEDDMWFLSNATVAFFPSFTSFIIMFNTMIPLSLYVSLEIVKLAQMFFLHTDVDMYDEVSDTPCEPRTSTINEELGQISYIFSDKTGTLTDNSMKFRKMSVAGVAWLHDADLRNAKQKKKLLHKTRKKGKQPVKNRKSVRSAKDVSPEDPSTPALEIRDPTDAAGDTAGSQWKSSARPGKASREYRTQDMLRYIQRKPHTPFSKKARMFLLSLALCHTCLPEVQEDGETHFMASSPDELALVQAAQDMGFLVINRDVHKITLKIMPSGAEGESVLETYEILDVIEFSSKRKRMSIILRYPDGRICIFCKGADSIVMQRLKLAALANKRMVEIERRANKRKSMEVQQAIARMSEQIDRKSSVGGRNSSVGGRKSMTLGQAARASMNIGRPSFARNSIAGRGSLSARDGMDQWLRERENDYSDMSSLDDDTPVHTPRQSGLDRLSMAFSEARSSIQLEELESMVDENIAGDDSAVIERCLQHINDFATEGLRTLLYGYRFLTEDEYQIWKKGYLEATTSLVDRTRLIEEAGDMIEQNLDLCAATAIEDKLQQGVPEAIDKLRRAKIKMWMLTGDKRETAINIGYSCRLIKDYSTVTVLDHETGEVEQSIGAAVLAISSGTVAHSVVVVDGQTLTQITEIPALDTLFHELAILADSVICCRASPSQKASLVKSIRKRVRKSITLAIGDGANDIAMIQEAHVGIGITGKEGLQAARTSDYSIAQFRFLTKLLLVHGRWNYVRTCKYTVGTFWKELLFYLTQALFQRSVGYTGTSLYESWSLSMFNTLFTSLPVIFMGVFEKDLSASTLLAVPELYTKGQRNGGFNVRVYLGWMFMASAEAMVVYFCMLGLYGQALFTEDDSIYALGSICFTAVVVLISVKMQFLETHTKTLTNALAIFLSVGGWFLWCILLSVSYNPASKIYYVRSAFVRGFGATSLLWWLSLLIILASALVFEIAVQALRAAWATTDEDVFQALERDGAVKRRFEEAAAGNCRRGGRGRGGDGWDGVGGEGEGEGGGEEGEGGSGVVETEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y079</accession>
    <name>A0A9W8Y079_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010885</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y079"/>
    <dbReference type="OrthoDB" id="3926908at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="139"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4361944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="188" mass="21281" checksum="6180A1AB7AE61F42" modified="2023-11-08" version="1">MSNQDAIMGNAPLTPGDEPEPEPQNPVVDFSPATVRYDESFENSLMELVLNPSEHKTSRQPSHEPHMIPASQLPIPLSSHLRTHTSPIPGLYLTHPNGYHTGGPGPASHTVKDFADKFIREHGIEDAGQLERVVEMMVRDKMKEVEERMEKRSEAVERNKGVERELEDLRLQRSAELRVMERFKGGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0H5</accession>
    <name>A0A9W8Y0H5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">pectinesterase</fullName>
            <ecNumber evidence="3">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_009565</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pectinesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0H5"/>
    <dbReference type="OrthoDB" id="2019149at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321">
        <property type="entry name" value="ACYL-COA THIOESTER HYDROLASE YBHC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321:SF137">
        <property type="entry name" value="PECTIN METHYL ESTERASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040969573" description="pectinesterase" evidence="6">
        <location>
            <begin position="30"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase catalytic" evidence="7">
        <location>
            <begin position="170"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4364110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="398" mass="43735" checksum="7A98A2650FF733AC" modified="2023-11-08" version="1">MRTSGSTGAFKNVMRVIAIIASLLEACVAISSINRKACQYPTKSPLDGCPKDTLLVGPGRSFTTVQSAILSLPNNTSPQHILILPGNYTEQVNVTRPGPVYLFGQTQSIDDQSKNTVNIIWRNATGAGVNSTLDNAYTSTLTVAPTFNASTTGSGPSGNPVPADTPFGNTDFRAYNLNFVNDYRNYATGPALAVSVSYANTGFYFCGFYSYQDTVYIGKLGNAYFYKNEIAGQTDFFYGFGTAWIQSSLVTLRNCGGGITAWKGTNTTFENKYGVYIHDSKVQKANSSLVLTGKCALGRPWNAQHRSIFANCYLDDSIQPNGYIEWSTTDPRVNFNTTMAEYKDYGPGWNATARAESNVTIVMDWKQYEPYSTVEKVFQYPFSGKFGNVAWIDRQPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y103</accession>
    <name>A0A9W8Y103_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008709</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Mycotoxin biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y103"/>
    <dbReference type="OrthoDB" id="1696280at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004165">
        <property type="term" value="F:delta(3)-delta(2)-enoyl-CoA isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000113">
        <property type="entry name" value="Enoyl-CoA hydratase/isomerase family protein (AFU_orthologue AFUA_2G14850)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF75">
        <property type="entry name" value="ENOYL-COA HYDRATASE_ISOMERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0843">Virulence</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="243" mass="27336" checksum="1EDFEFB0EB070449" modified="2023-11-08" version="1">MALGEYSNLRVDRYDRVFVITMQKAPENRLNVRFAQEIIRALRDIEQELGSDSEGCVITKGNDEKFWCTGLELDESDTNPFANSDGFFPLLATLLDYPFPTIALLTGHTFGGACPFALAHDYRIMNSSRGFFSMPPVDLGLHFPGIGYLPRLKLRPQIARKMLLEAYRWTGKQAYEDGIVDEVAEPKEMLDVALKKAREVQGKAKMGVYSVLRNELWGEAAEKIRGICYVHGRKVTAPAKAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y161</accession>
    <name>A0A9W8Y161_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Queuine tRNA-ribosyltransferase catalytic subunit 1</fullName>
            <ecNumber evidence="6">2.4.2.64</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Guanine insertion enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA-guanine transglycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_010018</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>guanosine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine</text>
            <dbReference type="Rhea" id="RHEA:16633"/>
            <dbReference type="Rhea" id="RHEA-COMP:10341"/>
            <dbReference type="Rhea" id="RHEA-COMP:18571"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:17433"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:194431"/>
            <dbReference type="EC" id="2.4.2.64"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of a catalytic subunit and an accessory subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the queuine tRNA-ribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.64" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y161"/>
    <dbReference type="OrthoDB" id="10249838at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008479">
        <property type="term" value="F:tRNA-guanosine(34) queuine transglycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00168">
        <property type="entry name" value="Q_tRNA_Tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004803">
        <property type="entry name" value="TGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00430">
        <property type="entry name" value="Q_tRNA_tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43530">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE CATALYTIC SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43530:SF1">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE CATALYTIC SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="8">
        <location>
            <begin position="128"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-guanine(15) transglycosylase-like" evidence="7">
        <location>
            <begin position="641"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA binding" evidence="6">
        <location>
            <begin position="876"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA binding; important for wobble base 34 recognition" evidence="6">
        <location>
            <begin position="900"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="720"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="895"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="720"/>
            <end position="724"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="774"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="818"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="845"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="933"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="935"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="938"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="970"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4362901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1010" mass="112994" checksum="B43ECDD83E69007B" modified="2023-11-08" version="1">MVKHPDMAEEEVIYPNRLQGHTIRLLELLPDDTEETPIQCRLIEASLDDDCPPFEALSYVWGDATITETIGCNGRDMQVTTNLAQALRKIRPRGAAAALDVEDRVEAGGRSGEEQPVPTTISSTRPPKKLFWVDAICINQKDVRERNHQVPLMKEIYSRAAGVTVWLGPANSELASRVASIIKQVCNHLLAYRMVVEQLRATNSAPSWLKGRGHFTFELCEASLRASGIENPWAALRVFFQLPWWSRTWCVQEVYLAQDVTLLFGTESIDGKNLTEFTQWFMRESTFENAKGLQSKCPSELNDTAVRTAYSRFNHRNLNARDLFMVCEDFRNLDATDARDKVYGLLGLWRPTGTEHASLDVNYGKSVAEVYTDVVLQAISARQDLFILRFVDHETEYRFDGHDGFPSWVPRWDHGEPWQFGWLDKSPLRASQYAAERPDQELARSGVLKVKGVLFDRVVWTSDVLEQGPISFLKDAAFRNRFIQLYKEASGMEPNPDFWHQISVPELATSISCGYIKMGDEMLDKDIDDPNAWLDVADLDSDTGRAFLDSFRTFMDTDTYPGDPKAFHKLMLCSNRRLFRTSRGYLGLGRRNMRPGDFVSVLHGGKAHAMRQSLPEANDTMAPVSMPSALKFELLGKCSVTKARAATLHLPHGPVPLPIFMPVATQASLKGLTPDQLEDQGCRLCLNNTYHLGLKPGQATLDAIGGAHKLQSWPHNILTDSGGFQMVSLLKLAQVTEEGVRFLSPHDGSPMLLTPEHSISLQNSIGSDIIMQLDDVIATTSPDAARMREAMERSVRWLDRCIAAHKYPERQNLFCIIQGGLDLELRRECTREMVARDTPGIAIGGLSGGEEKNSYCRVVDACTDLLPEGKPRYVMGVGYPEDLVVSIALGADMFDCVWPTRTARFGNAITPSGALNLRNARYATDFRPITASCTCPVCRPVSAGGLGLTRAYMYHVTAKETAGAHLLTMHNVHYQLSLMREAREAILQDAYPAFVRGFWHVVWRGEGEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1D0</accession>
    <name>A0A9W8Y1D0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4364964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1D0"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF31">
        <property type="entry name" value="LAEA-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G01930)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="49328" checksum="43E5904D5A795DB0" modified="2023-11-08" version="1">MASSSHVPDAITANTEGATHEDPATDTASSSEPSLQIDSDERDSAYGDNQSSYTMSATSSVFNFRYENGRRYHAYAEGKYPVPNDEVEADRLDLQHHAFRLTLDGRLYRAPIPEDVQCVLDVGCGTGIWSIEFADEHPSAHVLGTDLSPIQPAQVPPNCEFLIDDCEQDWIFHNQFDYIHTRAMVAAVKDWDRFFVQAYANLKSGCYLECQEIAFPIKCMDPGVTAENSVLIRWSDYFLEAAAKMGLDGTGPRHFAPKLRDAGFVNVNMKTYKWPVGKWAKGAKFKLLGRFVYEDLMDWLPSSSLGLFTRVLKWTREEVEVFLAECRTEAKRKDRHYYAEVLDWYALKPEGSSHPGPSHEPTQDEELLESDEEERGAHATEPNSSTAQALPIPTATDVTQASVEQLAAVALKDDDAPSVEQSLPLNTDVNAGETVSKTANDQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1L1</accession>
    <name>A0A9W8Y1L1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">aldehyde dehydrogenase (NAD(+))</fullName>
            <ecNumber evidence="3">1.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_009529</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>an aldehyde + NAD(+) + H2O = a carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.3" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1L1"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000049">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000007">
        <property type="entry name" value="NAD/NADP-dependent betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="7">
        <location>
            <begin position="20"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4364074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="487" mass="52441" checksum="372DFCD8DAFEC85B" modified="2023-11-08" version="1">MSVQYSTKNLPKQLFINNEYVDAKSSKKLSLYNPKDGSLLSDDVPLAGEQDVDAAVDGAEKAFPAWKKMSATQRRGILSKFADLIEQNAESIAQLSRISLGAPSAFGAFEIGLCAEAFRYNAGFIDKFGGESWPQEDGFLKIVRNEPLGVTAGIVPWNGPIGTIGLKAGPALATGNCFILKPSEKTPYSSLALGTLIKEAGFPPGVFQILTGDGSTGALLASHMRVRKVSFTGSISTGKKIQEMAAKSNLKRVTLELGGKSPAVVFDDCNLDNAVEWTANAITANTGQVCFAASRVYVQEGIYDKFIEKYKEAMKAKAKEIGDPDEKSTNIGPLVDEAQFKRVQGFIERGQQGQGTLAVGGKRVGTTGFYLEPTVFTDVDPKSEIHCEEIFGPVSVVKSFKTEKEIMELSNNTNFGLMAGVFTQDINKALRVASDFESGMVGINCVSLMFMSTPFGGSKESGLGRECGIHALRAFTEPKTIMINMNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y272</accession>
    <name>A0A9W8Y272_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y272"/>
    <dbReference type="OrthoDB" id="2322499at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="450" mass="50714" checksum="1146FF9971C6E002" modified="2023-11-08" version="1">MVNAKNVAFKVNKQTPFSQTLAGAYGTVINCRKPGGKGAARGQKEVRTHHVPLANVPGALKRKRGEEDCNEQRHPTTLSIPSGSDAAKSAVATLDQTLLNQQWQQRKPTRLRKGKILSKGVDLDCWFTILSFSDPAQLLEMRSKIASCYRFLRDNPMLWKHSRNYYYGPNMPDPPSQLSEFQYAHLRHGHGCMSCHAPNTRKTYWAFLRRWCKTCLQSKTLKEQDAMVLLRNANGEDITYLHKALPSGIFDSWGNFVGVGPASTHSLKTVYLLSDVQKLIAEYTQERQRNPEELAELRAWHAKKIDLVEERKAFAKKMELWEETTRNTKSFDYQAKKSARKAYYEHKAMQLSPPITLRELEACPSYRRAVAIPKDPNNTSWLQLKPKLEKEAADLAALRAQGGTPDPHPTMCLTSGTSTPLGSNTVPSQPFLPPLLGYPVGLPPPHSHLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2M9</accession>
    <name>A0A9W8Y2M9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Chromatin binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SWD1</name>
        <name evidence="7" type="ORF">N0V83_007911</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2M9"/>
    <dbReference type="OrthoDB" id="196858at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037850">
        <property type="entry name" value="RBBP5/Swd1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44040">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44040:SF1">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="63"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="449"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4366275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="488" mass="54920" checksum="C29838E5B04F45FA" modified="2023-11-08" version="1">MNLSLVDPFILAQDCPEVITGRLRSGHSTSIRFSHRGDLLASGRHDGIVVIFDIETNGVARKLRGHTRQVQSLSWSTSDRYLLSAGQDWKVVLWDLKDGSRVRTVRFEAAIFIAELHPKNHMLFVAALFEDQPVIVDMSTEVAVKHPLSSAPYRSQLEREGATEKQMAQDAKQTTTMTLFTPSGGHIIAGTNKGWLNIIDTETHQVRYSFKVTNNIIVYVRLTPSGRDVVINSSDRIVRTLHLPDLSDPKLDFDTLQLEVEHKFQDLVQKLSWNHVSFSPTGEYVTASTWMNHHIYVWERGQGSLVKILEGTKEELSVVEWHPFRPFVAAVGVDSGRVWLWSIPQPQRWSALAPDFVEVEENVEYIEREDEFDIQPLEEIHKRRLDQEDEEVDVLTIEPVKASSATDEFGPTTIGEADADFSMPVLLDIEASDSEDEVVAVGAGQFRRRSPGAGREWMNDDEVVVSGDEARRANGTAAQNGTKRRRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y359</accession>
    <name>A0A9W8Y359_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">J domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">JID1</name>
        <name evidence="2" type="ORF">N0V83_008451</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y359"/>
    <dbReference type="OrthoDB" id="445556at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="12"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="259" mass="30101" checksum="340B484F9A1567FB" modified="2023-11-08" version="1">MRAHIDPSAHGGDRDLLNWPEAIKPHKTPTPYQILRCGKGDAYSKNRFYALVKLYHPDRCHAASAVAHLPLHVRLERYRMLVAAHDILSDIEKRKAYDAWGHGWVGHHNTPAGAATHHHHSWEYDQRRWTSDPRYNATWEDWERWHSENDASKEPDGRTIQMSNFAFMSLIFAFVSIGGVVQGTRFSTLNNSVIERRDQIHREASMELRRSKDATMSGDRDERIRTFLEHRESTIMGEQSYQRLLPPADTCAPDEVRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3L6</accession>
    <name>A0A9W8Y3L6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">serine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Seryl-tRNA synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Seryl-tRNA(Ser) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SES1</name>
        <name evidence="15" type="ORF">N0V83_007619</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3L6"/>
    <dbReference type="OrthoDB" id="10264585at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000026">
        <property type="entry name" value="Seryl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="14">
        <location>
            <begin position="189"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="55"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="273"/>
            <end position="275"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="362"/>
            <end position="365"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="10">
        <location>
            <position position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4367089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="465" mass="53010" checksum="7723EF39A6534844" modified="2023-11-08" version="1">MIPNDFIKERGGDPEKIRESQRKRNAPVEIVDEIIVDFEDHRKTQYAATQIGSSINALQKQVGQKKKAKENADDLVAEMKKLQEDKKAKEEEANEKLRKLNSKAKTIGNYVYKDVPVSDNEDNNAVIKTWAPEDRKPEFKKDAIPHHGVLTRLNGYDPERGVKIVGHRGYCLTGYGLFLNLALINYGLEFLFNKGYTPNQPPFFMLRDQMAKTAQLSDFDEELYKVTENKDKPETDKYLIATSEQPISALHSEEWLQGEQLPIKYAGYSTNFRKEAGSHGKDAWGIFRIHQFEKVEQFLITHPEKSWEAFDEMLATSEEFYQSLGLPYQVVAIVSGALNNAAAMKRDLEAWFPVTGGGEYKELVSISNCTDYQTRELEIRHGAKKLNATRKEYVHALNGTLCATERTLCCLLENYQTPEGFNVPEVLRKYIPGQPEFLPFVKEWKAPKEDKPLPDRTKAPAPAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3N1</accession>
    <name>A0A9W8Y3N1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acireductone dioxygenase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Acireductone dioxygenase (Fe(2+)-requiring)</fullName>
            <shortName evidence="11">ARD'</shortName>
            <shortName evidence="11">Fe-ARD</shortName>
            <ecNumber evidence="11">1.13.11.54</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Acireductone dioxygenase (Ni(2+)-requiring)</fullName>
            <shortName evidence="11">ARD</shortName>
            <shortName evidence="11">Ni-ARD</shortName>
            <ecNumber evidence="11">1.13.11.53</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ADI1_3</name>
        <name evidence="11" type="synonym">ADI1</name>
        <name evidence="12" type="ORF">N0V83_007591</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 3-(methylsulfanyl)propanoate + CO + formate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14161"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:49016"/>
            <dbReference type="ChEBI" id="CHEBI:49252"/>
            <dbReference type="EC" id="1.13.11.53"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 4-methylsulfanyl-2-oxobutanoate + formate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:24504"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16723"/>
            <dbReference type="ChEBI" id="CHEBI:49252"/>
            <dbReference type="EC" id="1.13.11.54"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
        <text evidence="11">Binds either 1 Fe or Ni cation per monomer. Iron-binding promotes an acireductone dioxygenase reaction producing 2-keto-4-methylthiobutyrate, while nickel-binding promotes an acireductone dioxygenase reaction producing 3-(methylsulfanyl)propanoate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the acireductone dioxygenase (ARD) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.54" evidence="11"/>
    <dbReference type="EC" id="1.13.11.53" evidence="11"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3N1"/>
    <dbReference type="OrthoDB" id="1867259at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010308">
        <property type="term" value="F:acireductone dioxygenase (Ni2+-requiring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010309">
        <property type="term" value="F:acireductone dioxygenase [iron(II)-requiring] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02232">
        <property type="entry name" value="cupin_ARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000079">
        <property type="entry name" value="1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03154">
        <property type="entry name" value="Salvage_MtnD_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004313">
        <property type="entry name" value="ARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027496">
        <property type="entry name" value="ARD_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23418">
        <property type="entry name" value="ACIREDUCTONE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23418:SF0">
        <property type="entry name" value="ACIREDUCTONE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03079">
        <property type="entry name" value="ARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11 12" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 11" id="KW-0408">Iron</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="3 11" id="KW-0533">Nickel</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 11 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <note>for iron-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <note>for nickel-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <note>for iron-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <note>for nickel-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <note>for iron-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <note>for nickel-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <note>for iron-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <note>for nickel-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4367061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="176" mass="20677" checksum="DB5DDB43B214FF61" modified="2023-11-08" version="1">MKAYWFDNLPGDQREPHNSGRDVDPDYLAKLGVIYHNVPDVSGVDAIATSRNYKNRDEITVSPEKMGDIYEEKVKSFFHEHLHEDEEIRYILDGGGYFDVRSEGDEWVRIWLEKGDLIILPSGIYHRFTTDEQNYTKAMRLFKDEPKWTPLNRGNETDTNQYRQEYIKARQSLAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3Q0</accession>
    <name>A0A9W8Y3Q0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007664</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3Q0"/>
    <dbReference type="OrthoDB" id="10601433at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="326" mass="36998" checksum="E849A1055A339710" modified="2023-11-08" version="1">MRFFRRHYFQVSFMGLGFVREIIDTYFRKEPFRILHHELFTGRGNQEFFLIDKFGLGCPVGQLLQRVEVTITSIDLTIWDQLQPLMAIGNTDCAIELTSPTQLVTHDHYGQENPISAKEFLDKIALNVRRMTMKGYYITVRYEGEIIYTANDPDDKSRVSTEILTVMNTANRSRTNQCQSRKPSRVVNRPKPAALHKQPAKPAAQDPKLPNAPTRKEHGVPQPANEPPAGSKSVLKQHPVHPKIHQNHHGDLMDEDPKVPPKSAAPQPAVVCKLCGADVKTELPTTVADVARAKRTKRDGERPEDIKAFYEKRRAEVRALLAARQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y434</accession>
    <name>A0A9W8Y434_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SP-RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_008120</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y434"/>
    <dbReference type="OrthoDB" id="27975at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF4">
        <property type="entry name" value="TONALLI, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SP-RING-type" evidence="6">
        <location>
            <begin position="504"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="214"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="611"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="224"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4366484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="651" mass="73311" checksum="A63234F8F15D9117" modified="2023-11-08" version="1">MAGYYCMLSHIPNAVPEGLRCQPSLHNAMNMIRQVLDSNDTLSPAVLSLFCRFPYPLDVVGAKWPSQMQHQAELFLSFMTHSPNIDQMKRICRERRYPPLVRELAVDLRIASPTFRRLLFTSLLRSMWKSNPQTQLQAHYEAQAVETFQHNQATFCQQEAYAASVEPPFRLNHEQEKAQEAQRWAPQLKQIVDEFESRIRDHGVPLPSQYAAVHQQPYQTPHAQSQPSQPSVMVSRPSRPVITPSGQRGRGRPHPRPLLNLNPPAVPSQTQQNLSTPLLPPVGWTQPHQRLPNPARFSLHNAHLRSPILQAQSVPSSLYQFMVGWALSPTPVPDADREIARLTFTLSGDEMQIIPKTVPGAQGGPDIRTINDQSKTARLRSIKWSDTANTPRPSEHIWAVTDTTWIPYSFITFNGTSLHQRKKVHHGRDMPIDITGLLKEGENVLEVAVMAQSHETSHQNYLLAVEVLGFQTHNAIKQMCTAKRIPAAQVVDSIKRKLSGTSDDEDEICIVQSNLTVNLFDPFSASQPCDIPVRSTTCLHNDCFDLETFLQTRRRVADSSVADQWRCPICNADARPQRLFVDGFMQHVYQTLEAQGLGETRAIVVHQDGSWSPKAEVRDPNGVSDEPPSPTLPVARRTVTPRDNEIIDLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4V5</accession>
    <name>A0A9W8Y4V5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008561</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4V5"/>
    <dbReference type="OrthoDB" id="4332097at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="405"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="583" mass="64564" checksum="94374E0085B35F71" modified="2023-11-08" version="1">MSFYVYYGKLANELLVAVLPNGTVEHGDPIHLYTKKKFSSYKVKDVSVTDNGEELVAFNDGYYAYQAVSKKAYRELSLTIRKDGDSTAATVALVRHYDQPLTAIPLSDSPKIWTGAIDFQQWAKNESFIAIAPKGLGNGKPVVALWQWTEDAKGTPRTLSYWTGKQESEATNPTTFSFKQGDYYTLNCKVNAKTNGLNVTIKSPSNPEVVQKELALSAKVELGAEHSFTPPRPAQHQITLDCSLPRAAPSLPRITGALPFPAGLVDTLTYSAAYVDQAGYLAKYAVKQFDQLDKSFHQLEKKSEARAAKVTKLEGEIVRLGEANHVLTGQNAGLQKQLDANRVSAADAQKTLQKQLDDALKALVKTQSLNKELKSYIDADKLADIERERKHADHEAADHKAIDFANEALKKSHEVEQQLRQTLEKKNQTIVKLDASLETTRAQLGDANGNISRLNAELTVEKNERAEAVRKLGEEEYLRGSAEKQLKTSQALNVENADTIAKNETDINDQKEMLRQSNILFKTVTERLDKKDRELEAFKTSSKQRTAELQAQVDAADLHDRSHHVAAAVPKTNVSTSVTEIVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4Y4</accession>
    <name>A0A9W8Y4Y4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PLD phosphodiesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007302</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4Y4"/>
    <dbReference type="OrthoDB" id="9997422at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030572">
        <property type="term" value="F:phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00138">
        <property type="entry name" value="PLDc_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21248">
        <property type="entry name" value="CARDIOLIPIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21248:SF22">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="1">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="637" mass="71053" checksum="DAC6CCF950082162" modified="2023-11-08" version="1">MASRISDKIFQFCTDPNSVSALYARDPTQSPEDYAKKLYGGQHYAITEKKPPHERRALTKEDMDRARQCGNWGKTEPSELFLHCFHDALCSLEHDHLAGVISPSLMGSHGIIPLTILAPLPDIARHMANLIARAEKEVFLATNFWVSSNASRLITDSLLELSKRAGERGQRVVVKVMYDRGNIKQVVDNHQIMSVSQYTSDAVKLPSPDQIPHIDMEVQNFHRPMLGTFHSKYMVVDRRIAIVQSNNIQDNDNLEMMTHLEGAIVDSFYDVALLSWERAPKPPLPCHNDPAMAATTNTFSHPSFLGLFDENGKLKVPSAGNPDSLMGQGHEHQAMNTAGDPHYDTDIGAEIRRLQSALSPQNGETQMSKVSKHLNLATKLDLQGTAPDCPPDEEMTPMIPHKVHEPFPIAMVNRRPWGAINHACVNVPQNEAWLSALRNAKSSVFIQTPDLNAEPLIPAIKEAVRRGVEVTYYVCLGYNDSGELLPFQGGTNEMVANKLYLSLEDDERQRLHVHYYVAKDQIRPIHNKFKKRSCHVKLMIVDGHVGIQGNGNQDTQSWYHSMEVNIMIDSYEVCAGWLEGIARNQNTHQYGAASKEDGIWRDESGNEAEGAIGKDPGRFSWAKGIVGAVQRVRGAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y566</accession>
    <name>A0A9W8Y566_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS5m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MRPS5</name>
        <name evidence="12" type="ORF">N0V83_007261</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y566"/>
    <dbReference type="OrthoDB" id="309483at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000022">
        <property type="entry name" value="28S ribosomal protein S5, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000041">
        <property type="entry name" value="37S ribosomal protein S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48277">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48277:SF1">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S5 DRBM" evidence="11">
        <location>
            <begin position="264"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4367676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="428" mass="48151" checksum="27FAB63D22B186F4" modified="2023-11-08" version="1">MSVCRPARCLFSKATSTTLPKPCPQRSFHASAPRPARKRRPHYPSIKADDLKLLNQAAEAQYPKFDTSETTLLEKRYTPSQIAAIKAAESTIDARDVIIQGQRRTDPWRLPYEDDLAEVDPVTDKRERLGPDDIPGKKTFRLANEVERDEAIGRIATENLAKMYPEGVPEGQLDPVEEQKLQDAVEAALSQAALDPRATYTSKNAAAMEALADPRHSVVAPDLPRLENRMARQTRRLSTEEEEDPRQKRLLQYLGWDKQKLRSIRVKTLVVHGVTNQTRMGKIRSLYYLTIAGNQDGLLGVGEGKSVEPDEGRKQSVMSAIRNMRPVPRYENRTTFGDLEKKVGATKVQLFSRPPGFGLRVQHLIFELARAAGLQDLSAKTPRSRNKMNVIKATWEALTNQKLPDEIARARGKKLVDVRKVYYGGSIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5C3</accession>
    <name>A0A9W8Y5C3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Obg-like ATPase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">OLA1</name>
        <name evidence="6" type="ORF">N0V83_007041</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5C3"/>
    <dbReference type="OrthoDB" id="424823at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04867">
        <property type="entry name" value="TGS_YchF_OLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01900">
        <property type="entry name" value="YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.300:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.300">
        <property type="entry name" value="TGS-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00944">
        <property type="entry name" value="YchF_OLA1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004396">
        <property type="entry name" value="ATPase_YchF/OLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023192">
        <property type="entry name" value="TGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013029">
        <property type="entry name" value="YchF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041706">
        <property type="entry name" value="YchF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00092">
        <property type="entry name" value="redox-regulated ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF11">
        <property type="entry name" value="OBG-LIKE ATPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06071">
        <property type="entry name" value="YchF-GTPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006641">
        <property type="entry name" value="CHP00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="21"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="5">
        <location>
            <begin position="305"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="30"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4367458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="393" mass="43364" checksum="502B54326A7659A1" modified="2023-11-08" version="1">MPPKKAVKEEKLPLGRPGNSLKSGIVGLANVGKSTLFQAITKCSLGNPANFPYATIDPEEARVIVPDERFDWLVEHYKPKSVVPANLTVYDIAGLTRGASTGAGLGNAFLSHIRAVDAIFQVVRCFDDAEIIHVEGDVDPIRDLDIIAEELRLKDIEFVEKSLEKLVKETRRGGQSLEMKKLKEEQATVEQILAMLKDGKDVRKGDWSPKQVEVINPLFLLSAKPVVYLVNLSEKDYIRQKNKHLPKIAQWVKDNATGDPVIPISVQFEERLAAMSDDEAAEECKNIGTKSALPKVIVTMRKALNLGSFFTTGTDEVRQWTIRNGTKAPQAAGVIHGDFEKTFIQAIVYNYRLLKEEGDEAAVKAKGKIMTKGKDYVVEDGDILLIKAGAAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5Q3</accession>
    <name>A0A9W8Y5Q3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Inosine monophosphate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_007916</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5Q3"/>
    <dbReference type="OrthoDB" id="412383at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018580">
        <property type="term" value="F:nitronate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04730">
        <property type="entry name" value="NPD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004136">
        <property type="entry name" value="NMO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32332">
        <property type="entry name" value="2-NITROPROPANE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32332:SF20">
        <property type="entry name" value="2-NITROPROPANE DIOXYGENASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03060">
        <property type="entry name" value="NMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4366280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="36036" checksum="B9E74386697773A9" modified="2023-11-08" version="1">MPIKTWLREKLGIRIPLVQGGMMWVGRAELISAVANAGALGFLTALTQPTPEALRNEIRKTRSLLKASAVPFGVNITLLPMMNPPDYSAYARVVVEEGIKIVETAGDPSPILKYLKDNGVTVFHKCVTLKHALRAEKLGVDCISIDGIECAGHGGEYDTTSLMLLSLCAEQLKIPYLASGGFANGRQFAAALALGAAGVNMGTRWMCTVESPIHANVKDAIVKATENDTVLCLRKFRNTTRLHKNKVSVEVVKIENETRDVEFKDVAHLMSGKRGQGVYETGDVDAGVWSLGMCAGLIHDVPTCEELARRMEREAIEILTQSHALVVESAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6R0</accession>
    <name>A0A9W8Y6R0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006124</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6R0"/>
    <dbReference type="OrthoDB" id="5328813at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="132"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="517" mass="58715" checksum="7B9D769012C727A3" modified="2023-11-08" version="1">MPGEKKSSDLSSRLSVKRGAKLLTKTSKPVSSTISTPPKAAIAAPKAKKDEAPRRRNPFNDMLRHRSGEAADDDRDTLTPTSDREREPAACAPQASKVDTADRVAELERGLAIAKQEQDLLKEELEKFRQQWQARQDTFEGPKHQLSETQRRAEGASPRVSTEHERDNQVRRSSDRPGDDVFRQNYELRYRLVQAQEELVAQDAMYRDQLERRRSPHEETEWNELRTRLHATEKESQERLQQLLSLKSSISSLTRMDSQITDSELSESFSQLANRVREWVITNFRRSKLHIEHIPPETMKALMAVTPEYELAGRADRLALLQSVVSITLMDIFQEPFIVGLPKTGPLAAFRQCAHVIHGAGSEHREWRRVTIRALQKSDINHTIAEVKREVLHRLSRDVEHVLFTLTSASLTSGAQAGLISILNTAADLQHTLALQKAQYQVHFFHGQDGKTIELDGQRMEPVNDLDTALDEDGDTPTEQRFLFCVFPCLEKFGNEWGEHLETSNILLRARVCCGVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6Y0</accession>
    <name>A0A9W8Y6Y0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycine zipper 2TM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006289</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6Y0"/>
    <dbReference type="OrthoDB" id="3800349at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="42586" checksum="66A1C36E9B3CAAC7" modified="2023-11-08" version="1">MSVLAFKALGYGVEQIPDRFFEKIPGGYFTPNEKKDLDKHRKDRKHRKDKERRHSEQRSGRRSSRRDRTPPTDYSDYSANDDTDYERERERERRRRERRRAKSAGRTPSRSLSRGRHNRRESDFNGQQSDSRTMAQAGQGEPYFPPPPSSGDYKPYNPQEYAPSPTTSAYGYQPQPAAAQPYPAQPGGHRASSTTARYTPGPGYAPSPTVNPAVPQPVPYVPYNPADYPAGFQAPGNAYPSPPPLNRQRSSSQPSIPPGAAPYPTYTPPTSNQQMTPYVDQPSSRRDSTKPRREHRHRARSADSHSSHSRKHDQNRDDSRMAKVRERFDSMDMRERGLAATVGGALAGGLAGRQLGKSRLTALAGAAAGALGGRVISDKRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y750</accession>
    <name>A0A9W8Y750_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribosomal protein/NADH dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_006011</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFA2 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y750"/>
    <dbReference type="OrthoDB" id="10250268at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000219">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 10.5 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016464">
        <property type="entry name" value="NADH_Ub_cplx-1_asu_su-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007741">
        <property type="entry name" value="Ribosomal_mL43/mS25/NADH_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12878:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12878">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B8 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05047">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005822">
        <property type="entry name" value="NDUA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00916">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ribosomal protein/NADH dehydrogenase" evidence="10">
        <location>
            <begin position="20"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4368929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="94" mass="10698" checksum="FE5FE6ECC094B890" modified="2023-11-08" version="1">MASKYAFAQGLKELRFLFCQTSEHSAATRNFLTKSYPTMKKHNPHTPILIREASGTEPTVYARFDFGKEKKLPLKGLDDKAIEQQVTDLVHKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7T0</accession>
    <name>A0A9W8Y7T0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">intramembrane prenyl-peptidase Rce1</fullName>
            <ecNumber evidence="10">3.4.26.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RCE1</name>
        <name evidence="13" type="ORF">N0V83_005656</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Hydrolyzes the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where P1' and P2' are amino acids with aliphatic sidechains and P3' is any C-terminal residue.</text>
            <dbReference type="EC" id="3.4.26.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase U48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.26.1" evidence="10"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7T0"/>
    <dbReference type="OrthoDB" id="271604at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039731">
        <property type="entry name" value="Rce1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046:SF0">
        <property type="entry name" value="CAAX PRENYL PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046">
        <property type="entry name" value="PROTEASE U48 CAAX PRENYL PROTEASE RCE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 13" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="204"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="12">
        <location>
            <begin position="140"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4369892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="323" mass="36044" checksum="0C37452C39B43AD6" modified="2023-11-08" version="1">MKSNLWPVPKAPVISPSAAALLSAAYVSIYVIPFYLSSATRPSPTLTRDAPSSIRARTRAVTFSTLVCSVITVLVLYKHDVSATEIARLLGLWPVSLFDTARSMLLVAILFAGPLFEYGVVNDGWKDWIRLRGVHETLSSWIGYRNFIVGPVSEELVWRSCIVPLHVLAHFSGTQIIFLTPLYFGIAHLHHLYEFRITHPEVPLFVAILRSLFQFTYTSLFGFFAAFVFIRTGSVYTCMLAHTFCNWMGLPRFYGRVGVEAGVPIGPPDADKKDDKRRHALPFRGKSVAWTVAYYGVLVAGAAGFYYQLLPLTESKYTLPVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y898</accession>
    <name>A0A9W8Y898_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_005588</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y898"/>
    <dbReference type="OrthoDB" id="10027823at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520:SF5">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="96"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="281"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="359"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="434"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="27"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="225"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="468"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="486"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4369824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="498" mass="53632" checksum="D5EFE9F9CA66BA30" modified="2023-11-08" version="1">MANSLVRAGQWLALELGLETMRTAGKDVYIVIFARYIRLFAYGAVALVLAVYFKELGFSDEQIGLFMTLTLLGDVVISLLLTLVADALGRRRTLFLGALAMAVSGAVFATTSNYIALLMAAIIGVISPSGNEIGPFRAVEESTLAHLVPEAQRSDVYVWYVVLAVLGTSTGLAVGGVAVDQLQAIEGWTDLDAYRAIFWIYTGVGCVKAIMTLFLSRQCEHKDWADDHKSPTETEPLLHGNGNGNGSVESAESVSQEPAPAKPTKRFWHSISTISKPSRLVLLKLCGLFFLDSLGSGMVPFSLINYYMERKFELPKGKLGGIMSATWFVSTIGNVFAASLAKRLGLIQAMVITHLPSSIFLALLPVPPGLPLTICLLVGRSILNSMDQAPRSAFLSIVVLPGERTAVMGVVNILKTLSQSTGPSLTGLLAGHDRFWVAFVVAGSLKAAYDLMLLAFFGGRVHTRKDIGGDVDGSDDDSIADQSAEQQRDRPEEQGITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8W6</accession>
    <name>A0A9W8Y8W6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">KIN3</name>
        <name evidence="19" type="ORF">N0V83_006090</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8W6"/>
    <dbReference type="OrthoDB" id="10250725at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08217">
        <property type="entry name" value="STKc_Nek2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000697">
        <property type="entry name" value="G2-specific protein kinase nimA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000525">
        <property type="entry name" value="Serine/threonine-protein kinase KIN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF10">
        <property type="entry name" value="NIMA-RELATED KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="7"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="422"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="477"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="518"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="594"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="688"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="310"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="423"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="436"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="537"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="601"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ4369008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="739" mass="82094" checksum="B3F6DDE8C737020F" modified="2023-11-08" version="1">MPEDDKYDVLEKIGHGSFGIIRKVKRKSDGYILCRKEISYLKMSQKEREQLQAELSILKELRHPNIVAYFERDHIKASQDLHLYMEYCGNGDLGRVIRELKNKNQVCEEEFVWSIFSQIVSALYRCHYGEDPPPAGRNVMGLVANAKPVRDSRRPMILHRDLKPENIFLGDDNSVKLGDFGLSKILQSHDFASTYVGTPFYMSPEICKAEQYGPHSDIWALGCIIYEMCAKTPPFNAKTHFELIQKIKLGRYPDIPACYSAELRKVIASCLQTTPDHRPDTAALLNLPIVKLMRKEQEVVKLGQDLRDQKMLAARREKEAENAIARIRKEIDDTLRREWEVKARLEIELQVKLQTEQRVQRELANLQSKFEEEVNKRVEAALKKYPNRLSTSPKLAPRSNTPLMPATEQRALFPTESEPLVVNGSQSTVDTNSDFTSGTDISSLSLDDSTDEITVKAKPKRPSRAPLSRARTMFAQQPASPMDVQMAEPSPAPASLAGLSLSPRRNQQPRQNIFAAAKAGNTKWEAEVPPSPTEAEWNADFDDDDDLPVLPSPTRARSASGGRLGGDDPFKVLANAQAPLLKANQRLGSTPNLLAPKGTKPRPVSAVPVVATSPTRAKSKSAEALSTSPRKNGLGLATKSAPHVGLPSKKGNEAIRIQAMRNNGGIQGRTLVELQQARGIPVQAMSEDEGRKGFGYGGGRRSPTKGARSAGISPPAVWDPDTELEMPSPFIVRNKRMIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8W7</accession>
    <name>A0A9W8Y8W7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FMN hydroxy acid dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_004960</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8W7"/>
    <dbReference type="OrthoDB" id="25826at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF143">
        <property type="entry name" value="FMN-DEPENDENT ALPHA-HYDROXY ACID DEHYDROGENASE PB1A11.03"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="4">
        <location>
            <begin position="37"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4370442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="248" mass="27795" checksum="28176265169300E9" modified="2023-11-08" version="1">MSHESATQHHPKYTNHKPEQDAVEYEMQTYTRGLDFERPPLTFQASKWEVLACSRLSADSKGYVYGSAGVRETTDKNRAAFKKWSIVPRRLVKYDGFPDLKTEVFGEQLQVPIAMAPVGVLKIFNPQGEVAAARAAAKEAVPYILSTASSTSIEDTAKANGKDGQRWYQLYWPSREHDDITISLLKSAKDAGYTALFVTLDTYILGWRPSDMDNGYNPFMRADRTGVELGMTGLCGARVDAYYLSRDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8X2</accession>
    <name>A0A9W8Y8X2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">GroES-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_005882</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8X2"/>
    <dbReference type="OrthoDB" id="5363962at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003939">
        <property type="term" value="F:L-iditol 2-dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006062">
        <property type="term" value="P:sorbitol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05285">
        <property type="entry name" value="sorbitol_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045306">
        <property type="entry name" value="SDH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161:SF25">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G14390)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="11">
        <location>
            <begin position="37"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="10">
        <location>
            <begin position="193"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4368800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="394" mass="41818" checksum="92FE2CFA474817DE" modified="2023-11-08" version="1">MAAEKKPDLQDSRQIRASVLHGAGDLRIESRSIFPPGPTDLQVSIRSTGLCGSDLHYYRHYRNGDILVREPMSLGHESAGVVMAVGADVSNFKVGDKVALEVGLPCEQCERCKEGRYNICKGMKFRSSAKAFPHAQGTLQDRINHPAAWCHKLPDDVSLDLGALLEPLSVAIQASRRAQLGAGATVLVFGAGAVGLLVAAMAKISGAGTVVIADIDAGRVQFAVDNKFAHKGFTVPLKRGSTIEEQLDIAKETAAEIGKTTKESGGEVGEVDAVFECTGVPSCVQASIFASRPGGKVLLIGMGTPVQTLPISAAALREVDIIGVFRYANTYPTGIEVVSKKGPDYPDFSKLVTHRYQGLEAAVEAFDMAGKTKDDSGNLVIKVVLETGEEKSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y906</accession>
    <name>A0A9W8Y906_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aldose 1-epimerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_004680</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldose epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y906"/>
    <dbReference type="OrthoDB" id="274691at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004034">
        <property type="term" value="F:aldose 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09019">
        <property type="entry name" value="galactose_mutarotase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.10:FF:000014">
        <property type="entry name" value="Aldose 1-epimerase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047215">
        <property type="entry name" value="Galactose_mutarotase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091:SF6">
        <property type="entry name" value="1-EPIMERASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G13240)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091">
        <property type="entry name" value="ALDOSE-1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040926903" description="Aldose 1-epimerase" evidence="4">
        <location>
            <begin position="18"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4371463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="42852" checksum="FFD8A5B44EF0A2F9" modified="2023-11-08" version="1">MWFQSALISLLATTALAQYDTPNGPDEDGKYEISAEGIRGRFIPYGASVSNLFIKGKDGVERDVVLGFDNATAYEGEVHPHLGGVPGRYANRIKNSTFVIDGEEYHIEPNEHDNENTLHGGPDGWDYRNFTVVAHTNDSITFSIVDPDGKEGFPGEVVSYVTYTMTPNTWNIKIVALATTKKTPIMLTSHTYWNLDGFAKPNDLTALNHTLYMPYSGQRVSVDGILIPDGDILANQQYSVNDFWSAPKQIGANLTAPELLGNCGTNCTGYDNCYIVNRDQNGPYDWRTSEPVASLSSDWSGIKVDIFTDQEAFQMYSCGGQDGTLPYKSTQGVNGTAGFVPQYGCIVMEVQDWIDAINQPEWGRDKRQIFGPGDAAYTVNAEYVFSTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y964</accession>
    <name>A0A9W8Y964_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ATP-binding cassette transporter snq2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SNQ2_2</name>
        <name evidence="12" type="ORF">N0V83_004562</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002416">
        <property type="entry name" value="ABC multidrug transporter (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="634"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="667"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="709"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="741"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="774"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="879"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1307"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1340"/>
            <end position="1358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1378"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1418"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1449"/>
            <end position="1470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1576"/>
            <end position="1595"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="277"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="972"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="113"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="924"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="33"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="194"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="924"/>
            <end position="947"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4371345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1625" mass="180849" checksum="10009A619EC49F68" modified="2023-11-08" version="1">MASDEQPTPLVSHLPGGWTDTPADPSRHQSFIGSQNQTQESSTGGQQQAADGTFISNAYGLRSESYHGGEGAGLFSESSHSTAVPSPEPAPTPSATEYHTLENVTNLIPHRDVSHNELSPKPVETTGLAQQGYAVEPKEEVVVLPDDSSDSEHEAGGYAAIKSADSGRPALQSRQSRPMTEDDLFRVLSRRRTNQSNALGRSNTAATASSKEEEDEINNLMSKMFGRTRQEGSEEEKTRHQGVIFKNLTVKGMGIGAALQPSVGDFFLNPGRFVKNLISKGPRKAAGKPPVRTIIDDFTGTIRPGEMVLVLGRPGAGCSTFLKVIGNQRFGFEEITGDVTYGGTDAAEMAKKYRSEVLYNPEDDLHYATLKVKDTLRFALKTRTPGKASRNEGESRKDYVNEFLRVVTKLFWIEHTLGTKVGNELIRGVSGGEKKRVSIAEAMVTKASVQCWDNSTRGLDASTALEYVQSLRSLTNMAQISTSVALYQAGESLYDLFDKVLLIHEGRCCYFGPADRAADYFKSLGFVQPERWTTSDFLTSVTDDHERQIKDGWEDRIPRTAAQFGEAFAKSEQTQHNIAEIEEFERETKRLTEERNQASTKATKKKNFTLSFPKQVAACTQRQFLVMIGDPQSLIGKWGGIFFQALIVGSLFYNLPNTAAGVFPRGGVIFFMLLFNALLALAELTAAFESRPILLKHKSFSFYRPAAYAIAQTVIDVPLVLIQVLIFDIVVYFMANLSRTASQFFISVLFLWIITMTMYAFFRALGALVGSLDVATRLTGVAIQALVVYTGYLIPPSKMHPWFSWLRWINPIQYGFEGLLANEFYNLEIQCVAPYIAPQVPGAQEQYQSCAIQGNTPGSLTVSGSDYLEAAYQYSRSHLWRNFGFICAFFIFFVALTAIGMEMQKPNKGGGAVTIYKRGQVPKTVEKEMETNSLPKDEETGKTEPATEKQSASDNDESGKTVAGVAKNETIFTFQDITYTIPYEKGERTLLQGVNGFVKPGKLTALMGASGAGKTTLLNTLAQRINFGVVSGDFLVDGKPLPHSFQRSTGFAEQMDVHESTATVREALRFSARLRQPKETPLEEKYEYVETIIDLLEMRSIAGAAIGSQGSGLNQEQRKRLTIGVELASKPELLMFLDEPTSGLDSGAAFNIVRFLRKLADAGQAILCTIHQPSAVLFEHFDQLLLLKSGGRTVYFGELGHDSQRLLKYLESNGAKKCPPNTNPAEYMLEAIGAGNPDYKGQDWGDVWAKSSENAELGKEIQDIISKRRDAAKNEEARDDREYAMPYVQQWLAVVNRSFVAIWRDPPYILGVTMLHIFTGLFNGFTFWNLGQSQIDMQSRLFSIFMTLTISPPLIQQLQPRFLSVRGIYESREGNAKIYSWTAFVWGTILSELPYRIIAGTIYWCCWYFPPHFPRDTYTAASVWLFMMLFEIFYLGFGQAIAAFSPNELLASLLVPLFFTFIVSFCGVVVPYAGLPYFWRSWMYWLTPFKYLLEGFLGLLLRGQQIRCETKELAIFPAPPNQDCQTYAGQYAQQVGGYVQTQPDGTCGFCQYATGDAFAASFNVFPKNIWRDFGIMWAYILFNFAVVFVCTWLYLGGFRKIKSVFSPAARKQKKESKKRQSGDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y980</accession>
    <name>A0A9W8Y980_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">Chitin synthase</fullName>
            <ecNumber evidence="2 11">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CHS3_2</name>
        <name evidence="14" type="ORF">N0V83_004449</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Polymerizes chitin, a structural polymer of the cell wall and septum, by transferring the sugar moiety of UDP-GlcNAc to the non-reducing end of the growing chitin polymer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + UDP-N-acetyl-alpha-D-glucosamine = [(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9595"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="2.4.1.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the chitin synthase family. Class III subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2 11"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y980"/>
    <dbReference type="OrthoDB" id="26569at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013616">
        <property type="entry name" value="Chitin_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF11">
        <property type="entry name" value="CHITIN SYNTHASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01644">
        <property type="entry name" value="Chitin_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08407">
        <property type="entry name" value="Chitin_synth_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4 11 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="532"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="571"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="614"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="647"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="692"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="732"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="869"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin synthase N-terminal" evidence="13">
        <location>
            <begin position="134"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4371232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="901" mass="100901" checksum="AA732D3CD5256F7B" modified="2023-11-08" version="1">MAYNNQGYGDPHTGYDRDRHDEDEVGRSLLHENPSGTNPDPFYGNPEIRPHSTYSLTESYATERPTQYPGQAHDGSDIYSTTGGYNDPAAEFGAPQRAPSPYQRSETSSTEAWRARQQPGGGVAASGGLKRGMTRKVKLVQGSVLSADYPVPSAIQNAIQAKYRNDLESGSEEFTHMRYTAATCDPNDFTLKNGYNLRPAMYNRHTELLIAITYYNEDKVLTARTLHGVMQNIRDIVNLKKSEFWNKGGPAWQKIVVCLVFDGIDPCDKGTLDLLATVGIYQDGVMKRDIDGKETVAHIFEYTTQLSVTPNQQLIRPHDDSASTLPPVQMMFCLKQKNSKKINSHRWLFTAIGRILNPEVCILLDAGTKPGPKSLLALWEGFYNDKDLGGACGEIHAMLGKGWKNLLNPLVAAQNFEYKISNILDKPLESSFGYVSVLPGAFSAYRYRAIMGRPLEQYFHGDHTLAKILGKKGIEGMNIFKKNMFLAEDRILCFELVAKAGSKWHLTYIKASKGETDVPEGAAEFIGQRRRWLNGSFAAGIYSLLHFGRMYKSGHNIIRMFFFHIQMLYNIFQTILAWFSLASFWLTTAVIMDLVGTPTADKKAWPFGNDVTPIFNTVLQYLYLAFLGLQFILAFGNRPKGSKWSYLVSFFVFGLIQFYIVILSIYLVVQAFTNPKSQKLDTSSASAFANSFFSSSGVGIIIIALAATFGLYYVASFLYMDPWHMFTSFPQYLLIMSSYVNILNVYAFSNWHDVSWGTKGSDKADALPSAKTEKGADGKHTVIEEPDLPQADIDSQFEATVKRALAPYVPPVEKNEKTLEDSYKSFRTHLCTAWIGSNATLAVCITQDNFDRFVVSSTASSRTMHFFQALLWATAALAVIRFLGCLWFLGRTGLFFCVRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9T7</accession>
    <name>A0A9W8Y9T7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Class II aldolase/adducin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_004639</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9T7"/>
    <dbReference type="OrthoDB" id="2932980at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019323">
        <property type="term" value="P:pentose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050197">
        <property type="entry name" value="Aldolase_class_II_sugar_metab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22789:SF0">
        <property type="entry name" value="3-OXO-TETRONATE 4-PHOSPHATE DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22789">
        <property type="entry name" value="FUCULOSE PHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="4">
        <location>
            <begin position="35"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4371422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="296" mass="32575" checksum="B6E62F70E8EEF561" modified="2023-11-08" version="1">MGSTLQDTQAGAEVSPSTNLNPQVSTPASLRQQLRLLIRANHILHQNGLVDAFGHISIRHPIHQDQYITAAYDPGAPALVSSARDFITYHISDSSPVDANAPQGYSERYIHGEVLKRFPHANCIIHSHSEVVIPFTLGNIDVKPVFHMAGFMSSSPLPIFDISHTYANLPSYQPDMLIKTQPLGAALAEKLSDEQPVVLQHKHGFTTFGESVEEAIYKAIYTQANCRLLKDAIDLAGGEAGDVAYLTGEEAEACKVMNRRCQDKSFRLWLREVQVNPLYANDEGEPEKGEVAGMKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAT0</accession>
    <name>A0A9W8YAT0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA wybutosine-synthesizing protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_004139</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAT0"/>
    <dbReference type="OrthoDB" id="424465at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050910">
        <property type="entry name" value="JMJD6_ArgDemeth/LysHydrox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480">
        <property type="entry name" value="ARGININE DEMETHYLASE AND LYSYL-HYDROXYLASE JMJD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480:SF21">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="65"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="3">
        <location>
            <begin position="255"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="474"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4372365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="506" mass="56989" checksum="CD767CAF94EAA370" modified="2023-11-08" version="1">MAPGLLETPSVACAESRPSKKIKFSTAANFQIDDEDVAAAVPSHPLGIKPAGNAYTASENIKTRCGLFATLPDELLSNILESFDADVLIRLGSTCRALYAFTRLDELWRALFVGSPPDDFVWRGTWRATYLSIPKENITSISCNNLFSDTLYRPFQCAHTPLNHFARNIPKPNEIARLSDLTYEEYAETWTNKPFILTSPVKQWPIYETWTPEYLLEHFPDTRFRAEAVDWPMTKYMSYIHNNADESPLYLFDRAFAEKTNIDTTAPPHSKEASYWSPTCFGEDLFSVLGEHRPDCRWMIMGPARSGSTFHKDPNATSAWNAVLTGSKYWLMFPAGPGIEPPPGVIVSDDQSEITSPLSIAEYMLTFHELARQTPGCKEGICYAGEVLHVPSGWFHLVLNLEDSLALTQNFVPRKKLVDVLSFLRDQRGEVSGFKDDVCDRAYELFVERLREQCSDVLEEGLQELEKRGKKNKGRWEELTKGGQDEEEVGGFSFGFGGDDDDADIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC00</accession>
    <name>A0A9W8YC00_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004685</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC00"/>
    <dbReference type="OrthoDB" id="3668495at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="289"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="190"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="313"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="324" mass="36850" checksum="8EED5245A3C37D5E" modified="2023-11-08" version="1">MPNLPIKPSGVYNGAFIWDGHSLRECIKNATRSYDQDDPRTWPLFKDVHHFDSELEHRTKVPAPHSVAYDQSLMRSLLQKRSMKETRYNVTSGLCSGDAALNLRFRFYPFNFDQEGQLRDHPCIPPDDFECWMKFVHGSGWKVDRSSSTPQATTSEQQIPPTSTNITPSAAYEQIMKSLDASESARTVAVHGLEQELGNMKKQCQSLQYKAKETDQEIHELHNQIRSWKLKHAASDTEAQRLAAELSKGERRRADTEEKLKAYEARLKESQIELAVAKKEAEEANERIKTVWNKMQNGQQTTTQKRKTAAGVSGSQQKKPNTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCA6</accession>
    <name>A0A9W8YCA6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_004148</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCA6"/>
    <dbReference type="OrthoDB" id="4977at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028133">
        <property type="entry name" value="Dynamitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15346">
        <property type="entry name" value="DYNACTIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04912">
        <property type="entry name" value="Dynamitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="285"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="372"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="94"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4372374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="433" mass="48678" checksum="FAC205345858D69E" modified="2023-11-08" version="1">MSEGSRPKYDNLPGIDTAPDIYETPDLAEDVSTIQASTVVSESDGEDDKDPEEPERSAVHHQRLQTDQARNRFQPSRVDAHGVDFSDNITAQRRSYRTSTQRRRRRGEILGDDSDEEEESFAKKLLRIKRELAGLEHEYERRLESGDKSKIEEQDPKEMLEIIASKVDTIYAAHKGGARGADAILDRTVKKFDNYKPFEPSPKLTKAIANQPPLPGTQIQRSQLDFVLNQAAEFDKRITQLENNLGLNGNTMPELSESAPFPVFTTLTNLEQTLGLIGDASTNNMDAAIQQIKRLTADAEHLKEVRTEASRAGSSNGENNTTTYPDQEAKINALYGTLPSIDKLSPILPLVLERLRTLRLVHTSAWQADQVLSALERRQSKQESEIKRWERQLEIVERDMKKSEKAMLTNMKVVGDDVKMLEGKLAKLNAGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCE5</accession>
    <name>A0A9W8YCE5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002610</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the gemin-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCE5"/>
    <dbReference type="OrthoDB" id="428895at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032797">
        <property type="term" value="C:SMN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035426">
        <property type="entry name" value="Gemin2/Brr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12794:SF0">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12794">
        <property type="entry name" value="GEMIN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04938">
        <property type="entry name" value="SIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="320"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="435"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="354"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="510" mass="55898" checksum="4B7F504EF4B0DAC0" modified="2023-11-08" version="1">MANQGVNLAVTTDGGAYNKLPRPDDYLLMSTASHVPISGEVIPHAPYPAKRKRGQDVARGSKRVRATVSFAQTRSGDVNDEYNMNMKTALPGGNGEEQLSDNSTNEALAYICSVRSEASTIPRVLIATTSDNAIEEDQVNSPDEDIFNTSGEIYDRIDIGLERNPLASHEDDDGDATGSNPGAQQRCYKLLLRRFQALRDRILSAKRGSSYRNVAANSDNSTNIQLPSSKRAWLQMIERGWPSLDHILQIDDRTLYLGLQSCASSLSQSTTISREKSCWIWSLMASAGELGVLDRSRISKIRDLGLQAGRLSMWLRENSGDQGNVDENVDADGCDTKGADFGQHEGGSATVDHGNTEPHMVRESSQKETLEETDKAGHLSPEKGHGAPLKGDTSQSEADMSMSENEDKNHDKVHTTELEQARARLLAQLGDRLVQPEVPSSRAEAERLRDPAQGQAQSKHIDEDLSHETQTASLAMTEADWNTKAAIDMILTVAADCYGQKDLLSYREAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCF2</accession>
    <name>A0A9W8YCF2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003497</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCF2"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4373204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="147" mass="16522" checksum="D8E8678C353D9E94" modified="2023-11-08" version="1">MSSPVDSDDGIRYDWFTNMPPPSDDIEWNGLVQRGEKLFQTMMEPTATSAQSIFTDFPDVYDKWGYKTYALDSPHVKLGHQGITEAVEAIGASVDEKDWETHEVQHMPGYNQLRQGDGSTYQVGDKTYRFTGATFSSVINVKDGIVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCQ6</accession>
    <name>A0A9W8YCQ6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Substrate-specific activator of APC-dependent proteolysis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CDH1</name>
        <name evidence="8" type="ORF">N0V83_003041</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCQ6"/>
    <dbReference type="OrthoDB" id="10263272at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF1">
        <property type="entry name" value="FIZZY-RELATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="7">
        <location>
            <begin position="262"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="311"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="389"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="551"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="155"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="155"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="203"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4374300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="604" mass="66218" checksum="C4705414EC38EECD" modified="2023-11-08" version="1">MAQNEAASATAHNEQGIATPTLISPPESKTPPTASHKRNQLVFESNTHDRNSEAVDPSALAKALEQFEQAGRVRERTPTASPSRKRARIYGDRFIPNRAGQDLQASFNLLHDDGSPATPSKARRAPHNELHFQKTEEANRTYSAVLRQEMFENSVPQAFPQSLSPTDSANMRGAGRSHTPPARTTGSLPPPSLTPSTPHKNLFSYSGQPTPSRTPSSRQGVLNLNARSDLYSLSPIKYSSQRMLLSPQRAPRAVSKVPYKVLDAPDLADDFYLNLVDWGSQNMLGVGLGSCVYMWNSSSGRVTKLCELADDSVTSVNWIQRGSHIAIGTNRGQVQIWDAQTQRRLRTMMGHTARVGALAWNEHILTSGSRDRTIYHRDVRQPDQWLRKLVGHKQEICGLKWNHEDQQLASGGNDNKLMVWEKLNAEPTFKWSEHQAAVKAIAWSPHQRGLLASGGGTADRTIKFWNTLISSNGPSASSLAAASAAASASSSANVPISPTAPANLINSLDTGSQVCNLAWSKNSNEIVSTHGYSQNQIIVWKYPSMQQVVSLTGHTYRVLYLAMSPDGQVIVTGAGDETLRFWNAFKKKERTGGLSSMDSWGVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCR0</accession>
    <name>A0A9W8YCR0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002758</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCR0"/>
    <dbReference type="OrthoDB" id="2094269at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005645">
        <property type="entry name" value="FSH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050593">
        <property type="entry name" value="LovG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070:SF6">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03959">
        <property type="entry name" value="FSH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine hydrolase" evidence="2">
        <location>
            <begin position="39"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="335" mass="36466" checksum="450B9E30E429C451" modified="2023-11-08" version="1">MPRTLSHLIANTQLLSPTNLRIRSHWRRTMAAAPPPSRRIKLLMLHGYTQSGSLFQAKTGALRKTLSKAFPKGIELVYPTAPIRLTPADESWLAGTATDTTGGEQKQEQELDAWAWWKRKDQGGGNGPGGKGYTYEGIEIGLGHIASVLKEQGPFDGVVGFSQGGACAAMVASLLEPDRRAAFQAQVPKGGLPYPQSFESDDASGSSRAIHPPLKFAVSYSGFLAIGENPYQAFYDPKIQTPMLHFLGTQDVVVEEKRSLALVQACVHQEERYVVYHPGGHFLPSTQKASVNALIGFIKEVLHSEEEAAGAGKKEEESVEDMDVPFLSRGYYGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCR7</accession>
    <name>A0A9W8YCR7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATP synthase mitochondrial F1 complex assembly factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atp12</name>
        <name evidence="1" type="ORF">N0V83_003701</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCR7"/>
    <dbReference type="OrthoDB" id="5322896at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3580.10">
        <property type="entry name" value="ATP12 ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011419">
        <property type="entry name" value="ATP12_ATP_synth-F1-assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023335">
        <property type="entry name" value="ATP12_ortho_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21013:SF10">
        <property type="entry name" value="ATP SYNTHASE MITOCHONDRIAL F1 COMPLEX ASSEMBLY FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21013">
        <property type="entry name" value="ATP SYNTHASE MITOCHONDRIAL F1 COMPLEX ASSEMBLY FACTOR 2/ATP12 PROTEIN, MITOCHONDRIAL PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07542">
        <property type="entry name" value="ATP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160909">
        <property type="entry name" value="ATP12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4373406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="252" mass="27954" checksum="0BAADF27061B8598" modified="2023-11-08" version="1">MSIPVRMPNKQILTIPPSKPQLAAAIALEWDLLMSAQQALKTDYIPMTSLAARAIDLEAADAEGRDNVRNDILRYLMRVLSTDTLLCWAPEKVLNDSLQDGKTLRQLQEEMATRILGYLKTHVWPGVEIKPTLDPDSIIPVAQPEMTQQVIRGWCAGLPAYELAGLERAVLASKSLLVSARLIHEWSEAFAQSRKAADAVRFGIEEAAEAATMEVRWQTTQWGEVEDTHDVEKEDLRRQLGSAIILVGGNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDJ8</accession>
    <name>A0A9W8YDJ8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Signal recognition particle subunit SRP14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Signal recognition particle 14 kDa protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_002135</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of a fungal signal recognition particle (SRP) complex that consists of a 7SL RNA molecule (scR1) and at least six protein subunits: SRP72, SRP68, SRP54, SEC65, SRP21 and SRP14.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SRP14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDJ8"/>
    <dbReference type="OrthoDB" id="19209at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.10">
        <property type="entry name" value="Signal recognition particle alu RNA binding heterodimer, srp9/1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003210">
        <property type="entry name" value="Signal_recog_particle_SRP14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009018">
        <property type="entry name" value="Signal_recog_particle_SRP9/14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12013">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 14 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02290">
        <property type="entry name" value="SRP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54762">
        <property type="entry name" value="Signal recognition particle alu RNA binding heterodimer, SRP9/14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0733">Signal recognition particle</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4375055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="143" mass="16151" checksum="E7AD88748D725200" modified="2023-11-08" version="1">MARDHLSNDEFFTQLNNLFEHTRTKGHGSVYLTQKRFAFDSSSTPPSPAKIADDPLWDTHPEQPVPVLIRASNNKSDKRAGTDREDIEKIMLSTVVKPDDLDAFYVRYAEACKTGMSGLKKRDKKKAKKVKKKKKGTVGETKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDN3</accession>
    <name>A0A9W8YDN3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GH64 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001972</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDN3"/>
    <dbReference type="OrthoDB" id="10058186at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09220">
        <property type="entry name" value="GH64-GluB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.920.50">
        <property type="entry name" value="Beta-1,3-glucanase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032477">
        <property type="entry name" value="Glyco_hydro_64"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037398">
        <property type="entry name" value="Glyco_hydro_64_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042517">
        <property type="entry name" value="Glyco_hydro_64_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38165">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38165:SF1">
        <property type="entry name" value="GLUCANASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16483">
        <property type="entry name" value="Glyco_hydro_64"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52006">
        <property type="entry name" value="GH64"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH64" evidence="2">
        <location>
            <begin position="63"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS52006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="435" mass="46470" checksum="A5CD73EBAFF78C65" modified="2023-11-08" version="1">MKSFKKFINKRRQSFQNRNEGENNQTNPPANANANANANLSEKRAAAPQANGGQQQVLAAAPAGTLQLALQNKSSSSNVYAYITGLAIDKNNTPAFVQSDGRTIYYPVSPSTIQQPLQADVAIPLGAPGNTVTVNIPKIAGGRIWFSMDAKLTFLLNPGPAVVEPSVTNPSDPNFNIFWTFCEFTYNDAQLFANISYVDFVNIPVSLSLTNTQGTTQTVPGMAANGFQTVVDGLRAQAKLDGKPWDQLIYQVNGKPLRVLSPNNLLVGNDNAWGNYWDAYVQSVWTKYTNENITINTQAAAGNVTGRVQNGQLQLGAAGSFSAPTAKDIFSCSTGPFATGSNAARNAAIPRLAAAFNRSILLTTPGNQFPNGSNPSAYYKDATTNHYARIVHQVQKDGRGYAFPYDDVVPDGGSDVAGTIFDGSPKLFTIAVGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE01</accession>
    <name>A0A9W8YE01_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]</fullName>
            <ecNumber evidence="10">1.3.1.88</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">DUS1</name>
        <name evidence="20" type="ORF">N0V83_003409</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U47 in cytoplasmic tRNAs. Catalyzes the synthesis of dihydrouridine in some mRNAs, thereby affecting their translation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>5,6-dihydrouridine(16) in tRNA + NAD(+) = uridine(16) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53380"/>
            <dbReference type="Rhea" id="RHEA-COMP:13543"/>
            <dbReference type="Rhea" id="RHEA-COMP:13544"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:53382"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>5,6-dihydrouridine(16) in tRNA + NADP(+) = uridine(16) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53376"/>
            <dbReference type="Rhea" id="RHEA-COMP:13543"/>
            <dbReference type="Rhea" id="RHEA-COMP:13544"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:53378"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>5,6-dihydrouridine(17) in tRNA + NAD(+) = uridine(17) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53372"/>
            <dbReference type="Rhea" id="RHEA-COMP:13541"/>
            <dbReference type="Rhea" id="RHEA-COMP:13542"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:53374"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>5,6-dihydrouridine(17) in tRNA + NADP(+) = uridine(17) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53368"/>
            <dbReference type="Rhea" id="RHEA-COMP:13541"/>
            <dbReference type="Rhea" id="RHEA-COMP:13542"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:53370"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 5,6-dihydrouridine in mRNA + NAD(+) = a uridine in mRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69851"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:69853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 5,6-dihydrouridine in mRNA + NADP(+) = a uridine in mRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69855"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:69857"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Dus family. Dus1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.88" evidence="10"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE01"/>
    <dbReference type="OrthoDB" id="272303at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF5">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(16_17) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="19">
        <location>
            <begin position="37"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="336"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="510"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="378"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="520"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="549"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ4373118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="573" mass="64355" checksum="559A5BF2DBD05FE9" modified="2023-11-08" version="1">MMPAIESSSGVVQENGRPKKLRGRAFYESIGSPKLVLAPMVEQSEFAWRMLSRSFLPPSQQASLLAYTPMFHSKMFSEKSHYRDAHFQPLKSTIPSPVDDYHMSQVQERERHLDGNPAFDRPLTVQFCSNDPDDLLNAAKHVAPFCDAVDLNLGCPQGIAKRGKYGAFLQEQPDLIARMIRKLDEELTVPVTAKMRVLETQEKTLEYAKTLLDAGASIITVHGRRREQKGHNTGLADWTMIRYLRESLPKETVIFANGNILQHEDIAACLEATGADAVMSAEGNLYDPTIFAEPPHVGQEGREYWRGRDGKGGYRMDAVMRRYMDIIHKYALGQEPPERRPLWMPGDPVEGPAPEHPVVEEPNSSEEGPPKKKRKQMSKSEKKKEKTSNPNLTAMQAHLFHLLRPLVAEHHNVRDALARSRTGDIDAFENVLALTEKAVKEGIQKYELEQANTTDESAPAINEELEASGKAEEKLDPYESSLATVARCKRPYWVCQPYVRPLPKEAIEKGSMTVSKKERKRLEEEQELEKKAAGEKGRDVQAGDVGLEDGGRIEEERVGEQVVEEPREGVVCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEE3</accession>
    <name>A0A9W8YEE3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histidine kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_003366</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058846">
        <property type="entry name" value="PAS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF30">
        <property type="entry name" value="TWO-COMPONENT SYSTEM RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26131">
        <property type="entry name" value="PAS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13188">
        <property type="entry name" value="PAS_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAS" evidence="6">
        <location>
            <begin position="592"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="750"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="1122"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="163"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1038"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="188"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="1206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4373075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1312" mass="146201" checksum="118434592FECA12E" modified="2023-11-08" version="1">MATNEIPREQGEANQDTSTTPRRSIHPTSIPQLRRASTQDLASVTLLDALEYDDRPTFAVHVQAAQQRHVPLDFMYHNPAMQAVDGLLAKVSGHHDAMSMFDTAGAQLAFADWLGGRANEGDLARKANAYQFEGHIWTAVLVGRYKVVSGVPTALLWVDVVPGRQRGPTSPRERKEPTIRALQPDRASFTNDAGSTRSPPSLETPGAAIKYGPFDYTAETLPSTAASDPHIAYFRSVDWAQTPLGPMSAWPAELRAIVNHVLNDCFPNALFWGDDVILIHNQHYVQLLGALHPCMGKSIRTEAVDHWPSFEPIIKHINDTGRSLAEEDMLMFIDRHGFSEETHWAFQFVPVLDSDGHIAGYNQRFYETTNHHLLERRVSSLVEMASQTANARDFDSFWHIAQRTLTLNDKDVPFALLYAADRHGGTDTVPSPNSTPPLDKCLLKGAIGVESDHPIAPSVISVNEGSHIFQSFLQQAIKSRKATIVQLDELVSTGTTLQGIKWKGYGEPCRTIVICPILLTIGEQVVGFLILGINPRRPFDEAYQSFVQVTLRLLATSLASVLLLEEEKRQREMAIGQAARIQEHLLAELHLKEKKFQRFAERSDVGIFIMDRAGKYTYRNQRWYDLFGVSVKEDDVMTAWEQIAFPEDFDKFAAFFSKLVVDFEPICFELRTKLHWSPPPDLVQPECEATTTFKWILCSAYPEVDANGQICEIVGNITDISRQKWAESIQKVRTDSALEGKQHLEHFIDTTSHEMRNPLSAILQCADGILSSYSSEDDRKPPTPRLWSAFLEQTMDAAQTIAQCAQHMRHIVDDILTISKLDSGLLVITPVDAQPEAVAKHAVKMFEAEAKAADVDISYVVDQSYRDMQVDWVSLDPTRLLQVLINLLTNAIKFTRLEPTRRVTVSMAASATEPASVEGGIQFNEDRLVGQDSNLEEDWKQAPEVFFIQFCVIDTGRGLSEEERGNLFTRFSQASPRTHINYGGSGLGLFISRRLTELQGGAIGLASEAKKGSIFSFYIKTRRSKPAISRKGSIPNVIPEDIRHRPGTPLINLTRPAHPLRNLTGESDRRRTPPSCKAQTRHPFTRQVSMTHAQVSPEALGLPPDPDLKELKRSKSISETLHVLIVEDNLVNQKVLAKQLRNLGCVVSVANHGREALEFLPKTTCWDHSHPRSRALTQSYHIPSTEPIPDLVDGSPPVELSLILMDWEMPIMNGLTAVAQIRELEKDGVMRGRMPVIGVTANVRPQQIQTAVTAGMDDVVSKPFRVAELLVRMRGIVAGMTGDTEMKEVEDGVALALEAEAMNEKAPIDEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEE5</accession>
    <name>A0A9W8YEE5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RutC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEE5"/>
    <dbReference type="OrthoDB" id="309640at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00448">
        <property type="entry name" value="YjgF_YER057c_UK114_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="2-IMINOBUTANOATE/2-IMINOPROPANOATE DEAMINASE RIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803:SF58">
        <property type="entry name" value="PROTEIN HMF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="68" mass="7092" checksum="581206555EB512A3" modified="2023-11-08" version="1">MSPQAIQAAGQIFCSGQLPSDANGILIQGSIAQKSEQCIKNIKAILEEVGSGLDKVVKVTVSLARPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF61</accession>
    <name>A0A9W8YF61_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">geranylgeranyl diphosphate synthase</fullName>
            <ecNumber evidence="3">2.5.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">COQ1</name>
        <name evidence="9" type="ORF">N0V83_002404</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.29" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF61"/>
    <dbReference type="OrthoDB" id="9927103at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:geranylgeranyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046165">
        <property type="term" value="P:alcohol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043386">
        <property type="term" value="P:mycotoxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF69">
        <property type="entry name" value="ALL TRANS-POLYPRENYL-DIPHOSPHATE SYNTHASE PDSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4375318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="384" mass="41820" checksum="55488BF10EE9251B" modified="2023-11-08" version="1">MSRPPPDAPIKMDPFQTVAREMKFLTGNIRQLLGSGHPTLDTVAKYYTQSEGKYVRPMLVLLMSRATALTPRSSRAGGIGSKSVNNSITHPSILADENPDTSPISSIRHDNAYATEDSDILPSQRRLAEITELIHTASLLHDDVIDHSVSRRSAPSANIEFGNKMAVLAGDFLLGRASVALARLRDPEVTELLATVIANLVEGEFMQLKNTARDEKNPAWTEDTVSYYLQKTYLKSASLISKSCRAAAILGGSTPEVVEAAYLYGKNLGLAFQLVDDMLDYTISGEELGKPAGADLELGLATAPLLFAWKENGSLGKLVGRKFSGEGDVQRAREIVSKSSGLEQTRALAQEYVDKAIEAISFFPDSEAKNGLIEMCTKVMKRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFK0</accession>
    <name>A0A9W8YFK0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_001152</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFK0"/>
    <dbReference type="OrthoDB" id="655030at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46972:SF1">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46972">
        <property type="entry name" value="MONOOXYGENASE ASQM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="5">
        <location>
            <begin position="6"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4375874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="428" mass="47249" checksum="AF70FE092B7D0A78" modified="2023-11-08" version="1">MSSPLKIAIIGAGPAGLALARLLQINNITCTIYDLEADRNARTQGGCLDMHEGAAQQCIRECGLYEQFLAVARTEGEVLKIYEPNGTLIMDEGSDLGERRPDSFKGRPEIDRRQLRSMLIDSLYPGSLQWGHKVVAVKTDPTGRTAKYDLQFQNKLETGFDLVVGADGAWSKVRHLISDVRPQFSGITGVDAKLSNIDTVNPALAKRVGNGMCLTLGCNTTVLSQRNGDGSVKSYGFFRGTEDWHKTCGIDWSNVAEAKKEFVRKYYDGFNDDAKNLMLLADDDDFIPRPMWMLPVGHTWAHRQGLTLIGDAAHLMTPFAGVGVNVAMEDALSLATQIKKWKQNVWDKAGPSATSSLLIEPVRAYEEEMFVRAEAYAKETMMYYDLFFHERGGVAMCEQFAKAKAEEKAKGDVSVEVTELSLNTAVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFL0</accession>
    <name>A0A9W8YFL0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nonribosomal peptide synthetase 12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001167</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFL0"/>
    <dbReference type="OrthoDB" id="3142841at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052979">
        <property type="entry name" value="Adenylate-forming_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33927:SF5">
        <property type="entry name" value="ENZYME, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G01222)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33927">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="214"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="323"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="427"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="554" mass="61880" checksum="BEA8D62B1D28AEEB" modified="2023-11-08" version="1">MPHSFQPDIPTTSSNSLDTSSVISSVSSLSEDGLLHCKREPSSQQQLRNDTPNTSDNLYHQPSTADRQFSNSMTLPAPSHARDSALESPLQVQATAEKEGATSLTALPHITRNAHATVQEQTQASRFRRLQLHWLTAYRLLIGLTLAINLAVLIAWLNTYLPLAGLLQATSTNLFVAVLIRQEDLLNASFFLLAKIPLRLPLRIRKTIADFHHYGGFHIGCALSALMWYCVFVAANTIHTIKNVNRGNMTAWMWADIVTCYAFLTFILFICVTAHPRLRVKFHNTFEHTHRFGGWAAILVLWINSGVGTKAQNADQPLYQSPALWLLAATTFLIILPWLRIRRVPIHAALVSSREVKLTFPYPGMPYTSTTRFSLSPLMEWHAFATIPSDDGFTANIIISQAGDWTRNLIKNPPQRIWIRKPATANFLAFTPVFNSVLLVATGAGIGPMLSLLQSPAVAKMKTEGRQVRVMWCVYDPDAPHWKFVQEVIRKVDPMPKIFDSKQGRPDVVFEAMYMKHACDIETVMVVSNKKVTDEVVREVKAHGGAAYGAVFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG04</accession>
    <name>A0A9W8YG04_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG04"/>
    <dbReference type="OrthoDB" id="3799253at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4376114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="240" mass="27639" checksum="9D8B4349E80EB672" modified="2023-11-08" version="1">MPIMRKTTILDLPVEIIHTIRDFAAVASRACLALNCHHLLHYIGTRVFTDLDYNNTRLEKAAFLQTLQKDRLDPKLWLCYGCLRFHPVTQRYDPPNFGAVRGKEGVGFPKSVVVVRVWQHVGRLAYPFDQNESGEGHKSLYSDVAAPQESVVQKQMKLLPVLVDFRSKWRTISLSRGPIMRAVWRSRLKRMQKSKSEAAMVPMLAPAQQRQSLGEMKRRWESLAMLEATTLEEECKQYDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG65</accession>
    <name>A0A9W8YG65_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA polymerase</fullName>
            <ecNumber evidence="12">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">POL1</name>
        <name evidence="18" type="ORF">N0V83_000179</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="12"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6755010at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05776">
        <property type="entry name" value="DNA_polB_alpha_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05532">
        <property type="entry name" value="POLBc_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000004">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000003">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000036">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2820:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.10.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3200.20">
        <property type="entry name" value="DNA Polymerase alpha, zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024647">
        <property type="entry name" value="DNA_pol_a_cat_su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038256">
        <property type="entry name" value="Pol_alpha_znc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045846">
        <property type="entry name" value="POLBc_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015088">
        <property type="entry name" value="Znf_DNA-dir_DNA_pol_B_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00592">
        <property type="entry name" value="pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45861">
        <property type="entry name" value="DNA POLYMERASE ALPHA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45861:SF1">
        <property type="entry name" value="DNA POLYMERASE ALPHA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12254">
        <property type="entry name" value="DNA_pol_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08996">
        <property type="entry name" value="zf-DNA_Pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 12 18" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12 18" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase alpha catalytic subunit N-terminal" evidence="17">
        <location>
            <begin position="10"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="15">
        <location>
            <begin position="473"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="14">
        <location>
            <begin position="783"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger DNA-directed DNA polymerase family B alpha" evidence="16">
        <location>
            <begin position="1262"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="58"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="152"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="68"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="125"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="214"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4377354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1470" mass="164959" checksum="3C39440295B8E572" modified="2023-11-08" version="1">MSRAANRAKLAELKANRAAGKTRFSTYEVEEEEQLYDEVDEDGYKKIVRSRLDQDDFIVDDNGEGYVDDGREEWADGQDRYRYAATDSEEDDDRPAKGKAAKRKREEDKEKQEKINNGISKYFSAKNAAKAPKPKPVATAADKDFMDDLLGEIDSNPVRRSAPRMRPVKSEARRKTRVISPPVSENRPAAGKRRTNDSDAYMPDTPPAAHAYADDDGLPTFDDDVPMSDPPLQSSPVAKAVERRTQPAVKIEEEEEDLMEVAQPIGHLGVPAASVNMAGKRPIVKLKKADYPTPASSSPVGPSSAIDASSWNDVTSKLNVMSSPAPTTAGVGKMRPEDALEEDGSLRMFWIDYTEINGSLCLFGKVKDKKSGSYASCFMKVDNVLRKLYFLPREHRHVRGVATDEEVGLSDVSDEVDSIMSKHRVNLHKIKPCTRKYAFELPHIPKEADYLKLLYGYDKIPLSADLTGQTFSHVFGTNTSLFEQFVLWKNIMGPCWLKIDAPDFNAIHNASWCKLEVQVAKPNNISVLSNSDNLDAPPMTLMSLSLRTTFNAKDNKQEILMASAMIYDNFSLTDTTPVDEMQCKSFTIMRPNGAAFPTGFQAEVEKVKGNFMLKKTEQELLSLLMAMFQRHDPDVLIGHRLDDVDYSVLLSRLREKKTPGWHRIGRLKRSDWPKSMGKGGGSFFAEKQLAAGRLLCDLANDLGKSLMTKCQSWSLEEMCQLVLNKRREELDNEAALKSWATTKDGLLNYVKHCQADAYFIAAISMKVQMLPLTKVLTNLAGNSWGRTLSGTRAERNEYILLHEFHKNKYICPDKQWGKGKIKVEEEAAEGEEGADAKKKDKFKGGLVFEPEKGLYDKFILVMDFNSLYPSIIQEFNICFTTVERADLSEEDDKVPEVPTNQDQGILPRLIATLVSRRREVKKLMKAKDATPDQLATWDIKQLALKLTANSMYGCLGYTKSRFYARPLAMLTTYKGREILRKTKDMAEEKMLRVIYGDTDSVMINTNVDNIQDALKLGNEFKKEVNDSYRLLEIDIDNVFRRILLHAKKKYAAINMVPVDGKYIEKLEVKGLDMRRREYCALSKETSTELLNFLLSGEDPDTVVEKIHNHLRELSKQMREYTIPTRKYTIYTQLGKNPKEYPNGNSMPSVQVALRLQAKGKHVKAKDVMSFIITGESSGSAENAAKNAYPVDEVLKTDIGLKPDIEYYLHKQILPPVERLCAPISGTNITLLAACLGLDTSKYRVSTVSGGAQQDTEIHPLESQIPDSVRFQACTPLWLRCRTCTTVFPFQGLSSTSPTSMSLQQTVTHSGIKCPNSTCGTLLPNISVVAQLESAIRQVLAAYYAGNLFCDDPQCANHTSATRSISVYGHRCLGPKGLARDCMGKMSFEMKERDVWNQLLYFQSLFDVDRVAAGKGGVGVKVEEGSEKVKVLAEVNRERFGTLKGVVDRWLERNGRQWVQMDSLFSFALKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGQ4</accession>
    <name>A0A9W8YGQ4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">6-methylsalicylate decarboxylase</fullName>
            <ecNumber evidence="7">4.1.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_000154</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>6-methylsalicylate + H(+) = 3-methylphenol + CO2</text>
            <dbReference type="Rhea" id="RHEA:23112"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17231"/>
            <dbReference type="ChEBI" id="CHEBI:36658"/>
            <dbReference type="EC" id="4.1.1.52"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:23113"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. ACMSD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.52" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGQ4"/>
    <dbReference type="OrthoDB" id="2832284at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047596">
        <property type="term" value="F:6-methylsalicylate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032465">
        <property type="entry name" value="ACMSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240">
        <property type="entry name" value="2-AMINO-3-CARBOXYLMUCONATE-6-SEMIALDEHYDE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240:SF29">
        <property type="entry name" value="AMIDOHYDROLASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="9">
        <location>
            <begin position="8"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00036832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4377329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="347" mass="38255" checksum="7F112DCEB1A02A57" modified="2023-11-08" version="1">MTSLPTRIDVHSHFLPPFYRTALAENGHSHPDGMPGIPEWSRERHLEMMSTANVSKSILSVSSPGTHIVPGNDALGKNLTRQCNIYAAELKRAHPEKFGYWAALPLPSVDAALEEIDKATQEGADGFGLMTNYHGNYVGAPEFDPVFDKLNEVGATVFIHPTTPCIKLGNDTSSAQTTDAVPFGSRYPIPIFEFFFDTARAVINLFSTGTIDRCPNIKFIIPHSGGALPPIMTRFIQFSSVVPGGRVLDAATVRKQLEEQFYFDLAGFVFDGESGGDGQLKAFVEGFKISHEKLLYGSDFPFTQTQFVKMLADRMKDGLENLFSEKERLAIYEGNAVKLLQDGRSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH18</accession>
    <name>A0A9W8YH18_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bacterial surface antigen (D15) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_000679</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAM50/omp85 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH18"/>
    <dbReference type="OrthoDB" id="1724197at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.50:FF:000008">
        <property type="entry name" value="Mitochondrial outer membrane beta-barrel protein Tob55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.50">
        <property type="entry name" value="membrane protein fhac: a member of the omp85/tpsb transporter family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815:SF18">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY COMPONENT 50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY SAMM50 PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="Bacterial surface antigen (D15)" evidence="7">
        <location>
            <begin position="170"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="511" mass="55282" checksum="3B2F86CCDA9C19E0" modified="2023-11-08" version="1">MASPNEDDVFERLKNKTDVNEQKRQQDELNARLYAQHHKSQERLVELIGDNTSLPVTISSVRILHANRTRRTFLERVVNPLLSANRDEPYTLEEALKEVGEATDKLNRFGIFKSPISVYLDKPDQTIVSSSPTDVDVYISAFERGNYTIKTGTEAGASEADAYVHAELRNLLGGAETLNAHGSLGTRTRSAYSLAFDSPILSNPDLKFQVNGFASSTLKSWASHEEVLKGGNSKLLWRSKTGHQHELGYSGIWRQVTSLAENASPTVRADAGDSFKSSITHTWINDKRDYPLLPSSGYLIKTVSEIAGFGPLKGDVAFFKSEAESQVALPFGDTGITLTAGLRGGLLYPLTLGGNSGPQASRINDRFQLGGPNTVRGFRLAGLGPHDGPDAVGGDVYAAGGASLLLPVPRVGKETPLRIQAFINGGRLLSLKSQSSKKEEGLFGSGNEVQNSLKKTLSSLTQELPSAAAGVGLVYAHPIARFEINFSLPLVMRAKEEGRKGLSFGVGIEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH56</accession>
    <name>A0A9W8YH56_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000840</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH56"/>
    <dbReference type="OrthoDB" id="2830640at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4378010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="203" mass="23197" checksum="41FC86C3CD42E8E6" modified="2023-11-08" version="1">MEQAQMKPLISRLQQSQNHAFQPELAPICILDLAVIRLRTFCYDTYSDFLPIREAMHTNLYYSPAQDFQLPELTDMPRKLTALINAAAGSTGAIQGTLEILQSLDRRLQETQQQQQSQSDELVVVVEMRDYLAFLQQTLEGTRRKNEYLKESVQGIVQMVYAVLQQKDNELNLRYGADMRMVAVVTLLFLPGTFVATLFSASW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHE9</accession>
    <name>A0A9W8YHE9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">NADH-cytochrome b5 reductase</fullName>
            <ecNumber evidence="19">1.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">CBR1</name>
        <name evidence="22" type="ORF">N0V83_001194</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">NADH-dependent reductase for DPH3 and cytochrome b5. Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase, predominantly CBR1. By reducing DPH3, also involved in the formation of the tRNA wobble base modification mcm5s 2U (5-methoxycarbonylmethyl-2-thiouridine), mediated by the elongator complex. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>2 Fe(3+)-[Dph3] + NADH = 2 Fe(2+)-[Dph3] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:71231"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="Rhea" id="RHEA-COMP:18003"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:71232"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 19">
            <text>2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46680"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18 19">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer. Component of the 2-(3-amino-3-carboxypropyl)histidine synthase complex composed of DPH1, DPH2, DPH3 and a NADH-dependent reductase, predominantly CBR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="19"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHE9"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090524">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NADH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000032">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000019">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF184">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 18 19" id="KW-0274">FAD</keyword>
    <keyword evidence="5 18 19" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="11 19" id="KW-0520">NAD</keyword>
    <keyword evidence="10 19 22" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="21">
        <location>
            <begin position="40"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU361226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ4375916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="286" mass="30811" checksum="01159ABAE8A39797" modified="2023-11-08" version="1">MGSSFSLQDALPYAVAVAAIIGGFAFYSIGGGTRKVLKATEFQDFELEEKTVLSHNTAIYRFKLPRKTDILGLPIGQHISLAATIAGQPKEVVRSYTPVSSDEDKGHFDLLIKSYPTGNISKHVAGLQIGQKMKVRGPKGAMVYTPNMVRHFGMIAGGTGITPMLQVVKAISRGRAAGDKTEVDLLFANVNPEDILLKEDLDSLAAKDPRIRVHYVLNNPPENWTGATGFVTADIIKEKLPAPASDVKVLVCGPPPMVAAMKKATESLGYAKAKPVSKLEDQVFCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHM4</accession>
    <name>A0A9W8YHM4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GDS1 winged helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000711</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHM4"/>
    <dbReference type="OrthoDB" id="4150221at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057511">
        <property type="entry name" value="WH_GDS1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25318">
        <property type="entry name" value="WHD_GDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GDS1 winged helix" evidence="2">
        <location>
            <begin position="94"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="402" mass="43803" checksum="1F52B56B9E8D146D" modified="2023-11-08" version="1">MGYNTRRKSLSLPSLGIHLPGAPRSARSPPSTSDSQHIAKRRKHSHSASSASSPASPPRPTTLRFDEQRPKSSGRVADTPPPSPGGESGSTKVDTQGIDDEIVVGVIEQLEKTGNRPHLLKELATVLSPTIPIVESSANPAAIISSRLATYLKRNWTALSRCPLDKKLVGTHPKRVYYFLSTCPHQPIPADAGNVPAAARIISPSLSSAASEEEDMDARSRDRMSPSPELDLSDYDSNSSDPFSNQSHPPTTANIAHNRRAQSPPLEKDEREFTQTASFLQQRRRSQEAERERSTSASVEAAPTKDVTMEEASQSIEETEESAARKNSEAAAALFGQMEHAATFHTALAPSSPALKPTLHIEMPPPLFKPNEVKLEAGDDDWHMKSPETIELDELDDLFDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHS7</accession>
    <name>A0A9W8YHS7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000699</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHS7"/>
    <dbReference type="OrthoDB" id="1193027at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="225"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="418" mass="44715" checksum="ED513700D34372DC" modified="2023-11-08" version="1">MSLKIRDYPKAAPAGILQLEYHLQASKNAVWYDMSLVDCDASLGPENPRFCPLVKGGVWMYVKPGLGSVAGRIDVGAGARVPRSSPVLSAPTKSKRQCKWCKAGEEQQDSAAAASSSPVLSSPLSKRQCKWCKAGEKQQEQEQQDSEAAAASSSPVLSSPPIKRQCGWCKGGDEQQQEDEQQDSAAAAASSSPILSFIKRQCKWCKAGAGAGEEREQEQEHQDSAEAAASSSSVLSSPPIKRQCKWCKAGAGAGEEQEQEQEQEQQDSAEAAASSSPILSFLKRQCKWCKAGAGEHQQKQEQQGGSEAAACPEAKCSQGKCEGVYGWPGSFRDPTYNCKAGVDIVVEMCTEGPGMKTIGPPEDAETDDDDDGIDRKPRLSPHVTPPTKVQVSPPAFCHVVCWREKVARWVGAGGQGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIB7</accession>
    <name>A0A9W8YIB7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_001664</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-binding monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIB7"/>
    <dbReference type="OrthoDB" id="74360at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051209">
        <property type="entry name" value="FAD-bind_Monooxygenase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42877:SF1">
        <property type="entry name" value="FAD-BINDING MONOOXYGENASE STCW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42877">
        <property type="entry name" value="L-ORNITHINE N(5)-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4376381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="620" mass="70503" checksum="96815365E89034BB" modified="2023-11-08" version="1">MAPDLNSLGSGGQWDYSTPGSSGYNIPDIVYNDPSSRKLKVLTIGAGLSGIQIAYQIQKYTENVEHVIYEKNADIGGTWLENRYPGCACDIPSHAYTLNFALNPDWPRFFSYAPDIQNYLAKVVEVFDLRKYMTFNTEVIRSEWQDDTGKWKVTLRQKTASGETREFEEECDLLLYATGILNNYKWPGIKGINSFKGRIVHTATWPEDYQEEQWANDRVAVIGSGASSIQTVPKMQPHVKHLDIFVRTGVWFVQIANNFGQNKEYSQAERDEFRNDPKKLVAHAKDIENQVNGIWSGFYAGSEAQKTLQDTFRERMKQFIKNERLLEGFTPKFEIGCRRVTPGDPYMLAIQKPNVDVHFTAVDEITETGVIGNDGVEREVDTIVCATGFDVTYRPRFPVIGRNGVDLYEKWKDEPESYLGLGCPDMPNWIMFVGPTWPIENGSVTGPLLKVAEYTIQIIKKMQRDHIKSWAPKQSITDSFNAHAQEWIKHSVWKGSCRSWYKNNNTGRVNAVWPGSSNHYSEVIASPRYEDFEIEYMDEGGNMWAHLGMGYARCNVEYPEADVSPYLQLENIDPKWLKATGYRGAAGEVERAREVKERMAVERRAADGEGEEDVVVAQDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJF7</accession>
    <name>A0A9W8YJF7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gfd2/YDR514C-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000873</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJF7"/>
    <dbReference type="OrthoDB" id="5953249at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040151">
        <property type="entry name" value="Gfd2/YDR514C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048519">
        <property type="entry name" value="Gfd2/YDR514C-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28083">
        <property type="entry name" value="GOOD FOR FULL DBP5 ACTIVITY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28083:SF1">
        <property type="entry name" value="GOOD FOR FULL DBP5 ACTIVITY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21762">
        <property type="entry name" value="DEDDh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfd2/YDR514C-like C-terminal" evidence="1">
        <location>
            <begin position="48"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4378043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="233" mass="25858" checksum="449DFE708825C801" modified="2023-11-08" version="1">MAFNWYVSPVDNRYLTEYLQYYSDGDVLRHFLGAPIPLAPPLLNEATIICLDAEWYFKDPKPTTEIGIAELTVRGIAPNVHAENILSSIRVDHARIMPHAHLQNTFPGAGDPEAFYFGRTKFVTIPEARQVILNTFVRPRHIDNGELQPIILVGHAVDNDYDHIKEAFGIDLESYGTIVKVIDTQVMAKNANIEGPNGPNIGLKNLLAHFNIEIPNLHTAGNDAAGTLIAARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YK32</accession>
    <name>A0A9W8YK32_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor BYE1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">BYE1</name>
        <name evidence="9" type="ORF">N0V83_000546</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Negative regulator of transcription elongation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BYE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YK32"/>
    <dbReference type="OrthoDB" id="79252at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001139">
        <property type="term" value="F:RNA polymerase II complex recruiting activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031440">
        <property type="term" value="P:regulation of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21538">
        <property type="entry name" value="SPOC_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.30">
        <property type="entry name" value="Transcription elongation factor S-II, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055499">
        <property type="entry name" value="DUF7071"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012921">
        <property type="entry name" value="SPOC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036575">
        <property type="entry name" value="TFIIS_cen_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477:SF11">
        <property type="entry name" value="TRANSCRIPTION FACTOR BYE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477">
        <property type="entry name" value="TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23257">
        <property type="entry name" value="DUF7071"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07744">
        <property type="entry name" value="SPOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07500">
        <property type="entry name" value="TFIIS_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00510">
        <property type="entry name" value="TFS2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46942">
        <property type="entry name" value="Elongation factor TFIIS domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS central" evidence="8">
        <location>
            <begin position="270"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="143"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="401"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="714"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="177"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="226"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="416"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="459"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="714"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4377717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="861" mass="94314" checksum="D94A17528012A14E" modified="2023-11-08" version="1">MSEEIRRSGRANKGHHTKNQDALDEPSPAAPKPKAQPPKPESKKAQGPAKSQAKGQSARAQSTQSAEQDEGDEEEEDAIIRCVCGDQRDIRGRQMICCDSCLAWQHNKCLDLPEGDYWEGKDYYCEQCKPENHKELVAAMARGEKPWARKKGSKPKPRPSDIKQEAASEKATTPQQSATPSQAETAPAQASTPTPAPAPGPAQAQDASNGHTDPKGHKSQPQSPLGEKRRHEPTSEKANASKKRRKSSHHETKTTSQPTPAGDIDSLPQNQKALAEKLRETLTPLINTATDARGYRIPDGETAKSLATRMTLQISHAAFSQYGEPTGADSPYFAKLRTIMFNVKKNTVLVDQLLSGSLKAPEFVGMEAEEMASEDKQREYAAMREAAEKQMILTEEAGPRMRKTHKGEELVGEDSFDNHEFKQPNLRERESVDETAMQSPIAHRPSDSPAAAIRDPLSVDTSGTPQNDSARRPSTTFDINSVFDKVRSPQHDQQAFIQRRQSSMQMHDTAQTPVDDADVDRLLKDEDNDVEMSGYSSDPTVCWQGSISMQSNAPFDAVARFVAGGDFGQVVPWEKLLTNSLSVQGRIESAKGDDYIRGIGHTESHEVAILAVSPVTSDGRGLMDHLYTYFHSRGRWGVVPVDNLGNDIMRDLYVIPIEAGGSNLPPFIDMLEYCTIETPRKEHMLLLALIAKLPEVKPQLPATQHFERFPAQQTVAGQVAQPVSTTHVPTNGPSPSPVTNPHGPQFSPVSAAFPSGQYGNPYAQQPSNNGQQQPTIPPNAPPHHHIPRALEILGPYIDAPVIVQILANNISQNSVMSELQMGNLRHICDTVPEARTNISVFMDHLRQKAQANGQHAGTSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CGF3</accession>
    <name>A0A9W9CGF3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TAFII28-like protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010995</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CGF3"/>
    <dbReference type="OrthoDB" id="28335at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045127">
        <property type="entry name" value="TAF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006809">
        <property type="entry name" value="TAFII28_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13218:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13218">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04719">
        <property type="entry name" value="TAFII28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TAFII28-like protein" evidence="7">
        <location>
            <begin position="151"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="52"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="110"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4362053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="193" mass="20984" checksum="C501AC361422FC44" modified="2023-11-08" version="1">MASPPPVPHLNIPRKRSSISSISSANPNKRRKPSNLRNAFSPDAESVGSPRFSRSPSLDSVATTSVVNGTGGKKRRRKGDAGDGASVAGSSVRGGKGADGRSAVGGDGDGGQDYEEEEDDDDMGEEMDMAMEEGGMTVEARRKQEKDHERMLMEYMTPAQQERYATYRRIRLKRETVRKLVNQTLSQSVPNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH98</accession>
    <name>A0A9W9CH98_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ZZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_010982</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH98"/>
    <dbReference type="OrthoDB" id="661148at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14947">
        <property type="entry name" value="NBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02249">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02340">
        <property type="entry name" value="ZZ_NBR1_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032350">
        <property type="entry name" value="Nbr1_FW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930">
        <property type="entry name" value="OVARIAN CARCINOMA ANTIGEN CA125-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930:SF0">
        <property type="entry name" value="PROTEIN ILRUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16158">
        <property type="entry name" value="N_BRCA1_IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="6">
        <location>
            <begin position="390"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="520"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="748"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="823"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="520"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="537"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="849"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="876"/>
            <end position="899"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4362040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="943" mass="104424" checksum="B0AAE9D6C7DDD1B7" modified="2023-11-08" version="1">MATNVPVTLDTPIVVKLLFRGQTKKFKLPLKDLGAQVLPEKLRSILQIKPDDHVVFERYSDSAASYVTLDSSKPQVYKTLFRAAKAKLKLRLKATIPGEQVELPTPAPPAPAQEDIPSPLPSLHRLSSDTLTPQGIAPISPVIARSPGACPPAPKFEPISPVSPPKAAATEGEAPVPRSFTAHQGQYYAYSYYRTLTPPADFFTSFVATNHNSGLAFRPKPNNVCTSWVVYCNNCNQPMEDEHFHCSVCDGGDYDLCPACVDSGIHCPGTGHWMVKRFVKNGCVVNSTTQRIPPKVKAEEHHEVPGAFTDEKQPVAIEEEPTRTCNCCVKILPEKEFVTCVSCDDYDLCLECHVSNKHGHHPAHVFKAATAETVLPTLADFLCNSGRNVRHSAVCDGCDKFIYGVRHKCLNCPDWDYCSDCHKNAKFIHPRHRFVPIYEPLAEPLSSGNRHYGIYCDGPLCKEKENVSYIEGVRYKCAVCHDTDFCQNCEAHPSNKHNHTHPLIKFKTPVRSVSVTTVNDDRTGRSIARLGDRQPPRRSTATETTPVAPSTNAATQVQTVVDMKPSEEPQIKKEKIAIRDLLVEPVEPVQEKVVPLDMDDSKTVKTEAIPIPKSETSSHGLDAYFIRDTIVDGSKICAGQQFCQVWTLRNPGPNAWPAGCSVRHVGGDNMLNIDNTRPLSHTDLAEASESNTIGHYVEAGEEISFRVIMKAPAREGTAISYWRLKTADGMPFGHRLWCDIQVVAPPSPTSAQPEPVPAEPIASPEVAPTSTVSPLYERLLQARLERMKIVQQTHAQQLEQQKMERIQRVRKAAVERLLENRRKEHEAALSKSSPKVQVPTPEPTAELPVEEKPTERSLELHGSGMIFPQLDKESPESSTYQSSTTVQPESPTSEKSTIARTVTVASDDEFFEDAESVALQSDDESFMTDEEYDILDASDEEMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHL6</accession>
    <name>A0A9W9CHL6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphatidylinositol transfer protein (PITP)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PDR16</name>
        <name evidence="3" type="ORF">N0V83_010569</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHL6"/>
    <dbReference type="OrthoDB" id="75724at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000013">
        <property type="entry name" value="Phosphatidylinositol transfer protein PDR16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052578">
        <property type="entry name" value="PI_Transfer_CRAL-TRIO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824">
        <property type="entry name" value="GH16843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824:SF29">
        <property type="entry name" value="GH16843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="106"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="366" mass="40801" checksum="A2F97C7CE55D3A44" modified="2023-11-08" version="1">MSAIGPFAEPAPGCKPAAPPAVTADQQTKYDQLLADVRSWESLPTTNAKNAESAPLSDDERMWLTRECLLRYLRATKWNLHNATTRLRSTLIWRREYGTDKFTADYISEENETGKQVLLGFDNEGRPCLYLLPQNQNTKETPKRVEHLVYMLERTIDLHPPGQESLALLIDFRNTGAGGTPSIGLAKQVLDILQNHYPERLGRALLTHLPWYVTAFLRLVNPFIDPVTKSKIKHNEPLPNHVPSSQLMTVSGGDVDFKYDHSVYWPALEKLSNERRHERKDRWEKGGKLIGESEIYLWGGDEGSIGGGATHEETKLSENAVPPEVAEANGTTNKDDAALADGVTKLEVKDSEDANKSQAPPIPTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHV5</accession>
    <name>A0A9W9CHV5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">5'-deoxynucleotidase</fullName>
            <ecNumber evidence="8">3.1.3.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">N0V83_010324</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the dephosphorylation of the nucleoside 5'-monophosphates deoxyadenosine monophosphate (dAMP), deoxycytidine monophosphate (dCMP), deoxyguanosine monophosphate (dGMP) and deoxythymidine monophosphate (dTMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-phosphate + H2O = a 2'-deoxyribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18274"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:65317"/>
            <dbReference type="EC" id="3.1.3.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the HDDC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.89" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHV5"/>
    <dbReference type="OrthoDB" id="10254258at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002953">
        <property type="term" value="F:5'-deoxynucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009159">
        <property type="term" value="P:deoxyribonucleoside monophosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000011">
        <property type="entry name" value="HD domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039356">
        <property type="entry name" value="YfbR/HDDC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845:SF13">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13023">
        <property type="entry name" value="HD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0170">Cobalt</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="14">
        <location>
            <begin position="75"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4363204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="254" mass="28880" checksum="01A160FAF20CC092" modified="2023-11-08" version="1">MTADKAIQANGVSDPAAGKLDAKEAETSAWTVESVLAEITPRYAENTDSPVPFFHLLERLKTTKRAGWRRFGISDCESISDHMYRMSILTMMAPSSISSKLDILRCCRMALVHDMAESLVGDITPVDNVSKEEKGRRESETMDYICTNLLGKFNGGLNGKDVRQLWQEYEDSKTPESLFVHDIDKIELLLQMVEYERASGFERDLGEFTWVALKIQSDEVKVWAEQVFRERQDMWKQAGKTPSWRADTEPKAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHZ9</accession>
    <name>A0A9W9CHZ9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009711</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHZ9"/>
    <dbReference type="OrthoDB" id="3801489at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4363418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="287" mass="32802" checksum="6C24E9B262DCE127" modified="2023-11-08" version="1">MANNNLHPTPMASRLLTIPREIRDLIYSHLGNSISVEWTWPTEHLRTKNIFSPRLLPQAVRLQVQNAPQLSVLLTHSRLHDEYLEIVSRNGLSATVDLGRGGDWWSSHKASTELLDRLRHVNISLSVPFLSSPSLDNGPIMEDLADIINGKAPQLASLQVVLQSQYIYYESRDDFKDLFLPVIIKSTPPPIFGDLPLLNHAAGYRFNSQICPSRHSLLNNFERVCCYTYARNCNVQEAWIPGDILDRFNPDWSPHSKNLATITTETLGKGMDEWWEKRGYEEVKAWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIQ7</accession>
    <name>A0A9W9CIQ7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIQ7"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040860369" evidence="1">
        <location>
            <begin position="19"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="148" mass="13459" checksum="F96446947F6266F5" modified="2023-11-08" version="1">MRFSVLATTVLAAGAANAQVASIASSLAGEVSSLMGDGSSALASLTSAAGSLASSVTGSVGSDLASAASSIDAGASSAIASLTSEAATATGSRASEISSLTKSLASEASSLKSAASSATASSTGGAVPTALPAMGAVAGGLFALAGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJE0</accession>
    <name>A0A9W9CJE0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008934</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJE0"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4365314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="190" mass="20665" checksum="3D3BDE1B190AC857" modified="2023-11-08" version="1">MGDLNSKPQGQDASNMPAQYANLSKPANVPSVSGGYLWADETNKCFYQFGGEYTAGTTPSDFSMWTYDVLLNQWNSTEYTSSEKSLERLSFGAGTQADSLGLGYYFGGWLNNQTTPKWTGPGIATSHLVEFDFTKGILKNNTGPDDIGRAEGQLVFLPVSDKGVLIYFGGIEDPYHNGTSIAVRVTRQTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJH9</accession>
    <name>A0A9W9CJH9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJH9"/>
    <dbReference type="OrthoDB" id="28868at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.390">
        <property type="entry name" value="Hemimethylated DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011722">
        <property type="entry name" value="Hemimethylated_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036623">
        <property type="entry name" value="Hemimethylated_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032698">
        <property type="entry name" value="SirB1_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02097">
        <property type="entry name" value="yccV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31350:SF27">
        <property type="entry name" value="HEMIMETHYLATED DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31350">
        <property type="entry name" value="SI:DKEY-261L7.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13369">
        <property type="entry name" value="Transglut_core2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08755">
        <property type="entry name" value="YccV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00992">
        <property type="entry name" value="YccV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141255">
        <property type="entry name" value="YccV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="7"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="602" mass="69312" checksum="474A07D0D7DE8C48" modified="2023-11-08" version="1">MDATPPRPSFTDLPTEILEAIFLHIDSYSLISVSLTSKLLKQLTTDAPLIWRHICRTRFKTWDSRHNIAAKYAGPLSDVDWRSLFLQKITIEKRTRELLDHIVATQHERIRCINEIADFGYDAKETLLRECACPDDAEDVLARRYYANAVLERIHREIAINVWKAMLDGEQVPIEKALGAYDVFARVGEDVDIDVVSEDIDHLAAGVLEEYPAFSDLSPRMKASTLASYLRDHDFKGVPDTSYRALRNSFIGLVLRSPKHESLPLISVAIYCAVAQRLGLDARPCGFLFHVYTLVYAPKNYNLDGEYKPSSTPELDYMYLDPFRSSTEVRQGDLQRVLRDMGVPTVEYAQFLSYTTSREMVLRTARNIMNSVQTIRQTEAGVRGIQASWFNAYPDMDNAFYATIWAMLLLGPTDDAPSNLSTTTTRRRQYLPYLLEHFQTHFPWDVTLLSRYVIPMFYNQPEGHRLLQFVQSMHQVDAMRKPVLQRSERTVNVNFKVGQLFQHKRYSYEGVITGWDVACDAGEEWIQNMGVDRLPNGRNQAFYHVLVCDKSVRYVAEENIHPVDPATRPSEAILKLAGRHFKRWDDAEHRFVSNVRDEFPHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJP6</accession>
    <name>A0A9W9CJP6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_007755</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJP6"/>
    <dbReference type="OrthoDB" id="432970at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="5">
        <location>
            <begin position="8"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4366120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="248" mass="27820" checksum="E6C75EB3DD5B6B76" modified="2023-11-08" version="1">MADETPACANCEKTAAAANLASLKACAKCKTTQYCGRDCQKADWKTHKKICAKNASEAFVEANAEHSNTYSAGRLKDLEKHEPKPFTRLDEGKYLHDRPEKDVFKLLIDSFRMRQADDFNLEHKTTPNSVYAGASSSIVPFRQYLAKAATRRNILPSWWTADKQKECEAFGESGAWSDLRRKVTKQEMIQHYGDDKMPMQVRMLAEAVYGVGTMGQDGSGARRMMMRMENGGSGNDQIMSMLNLARGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJS0</accession>
    <name>A0A9W9CJS0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyruvate decarboxylase</fullName>
            <ecNumber evidence="4">4.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">N0V83_008539</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2-oxocarboxylate + H(+) = an aldehyde + CO2</text>
            <dbReference type="Rhea" id="RHEA:11628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="EC" id="4.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Binds 1 Mg(2+) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.1" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJS0"/>
    <dbReference type="OrthoDB" id="3970464at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004737">
        <property type="term" value="F:pyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000949">
        <property type="term" value="P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02005">
        <property type="entry name" value="TPP_PDC_IPDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07038">
        <property type="entry name" value="TPP_PYR_PDC_IPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000019">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000024">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012110">
        <property type="entry name" value="PDC/IPDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047214">
        <property type="entry name" value="TPP_PDC_IPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047213">
        <property type="entry name" value="TPP_PYR_PDC_IPDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452:SF30">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE ISOZYME 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036565">
        <property type="entry name" value="Pyruvt_ip_decrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="16">
        <location>
            <begin position="15"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="14">
        <location>
            <begin position="211"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="15">
        <location>
            <begin position="409"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>1</label>
            <note>substrate; ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>1</label>
            <note>substrate; ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>2</label>
            <note>allosteric activator</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="478"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="484"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>1</label>
            <note>substrate; ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4365917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="576" mass="63282" checksum="7C73B9FFC978BCA4" modified="2023-11-08" version="1">MAQDLRSQEIKNPVDVAEYLFRRLKEVGVESVHGLPGDYNLVALDYIPKTGLRWVGNCNELNAGYAADGYARVKGISALVTTFGVGELSAVNAIAGAYSEYVPIVHIVGYPSTLSQKNGALLHHTLGNGDFTVFSRMSKEISCAVSMLNNQHEAAVLIDNAIRECYLQSRPVYISLPSDMVQKKIDGDRLNTPLDLKYAPNDQEAEDYVVDVVLKYLHAAKNPVILVDACAIRHRALKETHELVEKSGIPTFVAPMGKGAVDETLPNYGGVYAGDGSNKGVRERVESSDLVVSIGAIKSDFNTAGFTIRMSQMNTIDFHSYGVKVRYSEYPGVRMNGVLAKVAAKLGKLNIEAGPKPNNDIPQREASSSEPTVTHAWFWPRLGQWLQKNDVLITETGTSNFGVWETRFPAGVNAISQVLWGSIGYATGATQGAALAAKELNIKRTILFTGDGSFQLTAQEVSTMIRNKLNPIIFVICNNGYTIERLIHGWQDEYNDVPEWKYKELPRVFGAEEGSVLTYRVSTKEEVEKLFEDQEFSSGETGKMRFVELVMPWDDAPAALKGVAEAAARTNARLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK88</accession>
    <name>A0A9W9CK88_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_007390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK88"/>
    <dbReference type="OrthoDB" id="433738at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46035">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46035:SF3">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4366862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="201" mass="21936" checksum="0EAB53FCF7345A4C" modified="2023-11-08" version="1">MESPDTFTLLPIEMDPTTKAISATNNLSNALTTELAALNSLHKGLLNVETPTGTPPPPVPVNPKRSAQIGKLRESGNAEFRKGNHGQAVKLYGLAIDMALGRPHWEPSGLVREEVSGLLSNRAQANMAQQNWAEGAVDAEASVEMKKVGNAKGWWRRGKCLLEMGRLDEAEQWVKQGLEFEATEQDLVQLRDEIEKKKGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKH3</accession>
    <name>A0A9W9CKH3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006840</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKH3"/>
    <dbReference type="OrthoDB" id="5126878at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053178">
        <property type="entry name" value="Osmoadaptation_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38111:SF9">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38111">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="10"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="474" mass="52823" checksum="E3C26119DF024567" modified="2023-11-08" version="1">MVGVAGKSKACNDCKRRRVKCGLERPGCTRCVRARIQCSGYAQQIFFVNKTLADPSVSAPIVLAESRLSKREHVKKNPLQDELDDLIALAKASLASPSLFRQGVFKLLQKLYLPQPHVADKNAGHVDPHTWLKAACDLEGPCLVLDNSLIAFCAIQLFVTKTGTVTHEKAVEIYNTTLGYLSTYLMRKGDMNLDYILASLAVLSTCELFLFPKDDGLQVHVQGIADILRLRKEQRGSLSAAQWSDLMPTETNLDPVDQLMDLVCDMPQAIHSAIKFSSMKQTVGGEFQVAVKSLWGILKSIYEWQAGLCALSTTPLYMAVPSRLHNQSDDSHETKLFPFALEFRSVPTAALFVVSWGFQLHIYTVLQQLRQEKGDGGPPHYVSIDPHTDPMEARIEANKMARFLCQSVEYCHRVEMGTFGPQTMIYSGWILRHYFTQNGAKRELEWVDNIESMSGDGVRGGIKLMKFQGGIGGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLE3</accession>
    <name>A0A9W9CLE3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidyl-tRNA hydrolase</fullName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_005842</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PTH2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLE3"/>
    <dbReference type="OrthoDB" id="1733656at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02430">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1490.10:FF:000001">
        <property type="entry name" value="Peptidyl-tRNA hydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="entry name" value="Bit1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649:SF11">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="Peptidyl-tRNA hydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="36"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4368760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="195" mass="20677" checksum="5FBFEC573A789CA1" modified="2023-11-08" version="1">MQDSSQPSTANIAVACAIIAGVTGYMLGQAKSLGLFGGSPVSQPQQRKDKKADTSDDEESDEEDDDTAPAEFPGHPEECKLVLVVRTDLGMTKGKIGAQCGHATLACYKHFLRHSPNSTILRRWEQMGQAKVALQVKSEEELELLQAQALSLGLVAHIVHDAGRTQIASGSATVLGIGPAPRGVIDQVTGHLKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLH7</accession>
    <name>A0A9W9CLH7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006278</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLH7"/>
    <dbReference type="OrthoDB" id="5231984at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040849111" evidence="2">
        <location>
            <begin position="22"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="311"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="356" mass="36571" checksum="639E16946BBCA9B3" modified="2023-11-08" version="1">MAFKYFRHVAFAAAVLSVTRAQQSSTIVPQDLQTGFKSSDKQVQVSYTNDAVEGFVDGTSFDKDAVTKEPTFALGDSSGITPSTLYTMIMIDTTCPNARKLHYARANFKNDFNVVNIETQSAALLDYKAPGSFGETGDNRQYTFLMYTNPARKQIDQLKLPADGENFDVKQFQSDNGLNDASAGVGMVVKLGGTADCGSDQSNSAPAAQSSTAQASSTDAGLASTAVSSAAEQQSSSVASATQTEDASQSGETVSPSSSALDGSQVGNSTSPSTTSARQPSDSVAATSVLQTDAPGPSTITSTVVLSSRPGGASASGSQTASASPALQTQNAAPRLATGHGVVMVPLLAVAGVLLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLW2</accession>
    <name>A0A9W9CLW2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13">Tryptophan synthase</fullName>
            <ecNumber evidence="5 13">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TRP3_2</name>
        <name evidence="15" type="ORF">N0V83_006325</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the TrpB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the TrpA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="5 13"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLW2"/>
    <dbReference type="OrthoDB" id="10050244at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06446">
        <property type="entry name" value="Trp-synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04724">
        <property type="entry name" value="Tryptophan_synthase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000151">
        <property type="entry name" value="Tryptophan synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000001">
        <property type="entry name" value="Tryptophan synthase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000004">
        <property type="entry name" value="Tryptophan synthase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00131">
        <property type="entry name" value="Trp_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006654">
        <property type="entry name" value="Trp_synth_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018204">
        <property type="entry name" value="Trp_synthase_alpha_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002028">
        <property type="entry name" value="Trp_synthase_suA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00262">
        <property type="entry name" value="trpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00263">
        <property type="entry name" value="trpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077:SF3">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00290">
        <property type="entry name" value="Trp_syntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00167">
        <property type="entry name" value="TRP_SYNTHASE_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="11 13 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 13" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="14">
        <location>
            <begin position="382"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4369240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="727" mass="78224" checksum="7A17F2A32F37E85C" modified="2023-11-08" version="1">MDGIKKTFAQCKKEGRSALITYVTAGFPTAQETPDILLGMEAGGADIIELGMPFTDPIADGPTIQTANTQALKNGVTTHDVLQMVRDARKRGLKAPVLLMGYYNPLLSYGEEKMLQDAKEAGANGFIMVDLPPEEALRFRNFCRSYGLSYVPLIAPATSEHRMRVLCKIADSFIYVVSRMGVTGATGTLNAALPQLLERVHKYSGNVPAAVGFGVSTRDHFLSVGKIAEGVVIGSQIVNTLINAEAGKGAKAVEEYCDKICGKSSRTTTREVGIVEALSEAKEPNGVHVDKVITDADTPDGPGLADQLEALNTNGTNGEQNGFDEEHAHPPRFGEFGGQYVPESLMDCLSELEKGFSQAVDDPKFWEEYRSYYDWMGRPGHLHLAERLTEHAGGANIWLKREDLNHTGSHKINNALGQILVARRLGKTEIIAETGAGQHGVATATVCAKFGMKCTVYMGAEDVRRQALNVFRIKLLGASVVAVEAGSQTLRDAVNEALRAWVVNLDTTHYIIGSAIGPHPFPTIVRTFQSIIGNETKQQMQEKRGKLPDAVVACVGGGSNAVGMFYPFAKDPSVKLLGVEAGGDGVDTDRHSATLSAGTKGVLHGVRTYVLQNKHGQISDTHSVSAGLDYPGVGPELSNWKDSDRAKFVAATDAEAFIGFRLMSQLEGIIPALETSHAIFGALELAKTMTKDQDVVICVSGRGDKDVQSVAEELPKLGPKIGWDLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLZ3</accession>
    <name>A0A9W9CLZ3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLZ3"/>
    <dbReference type="OrthoDB" id="1875589at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="416" mass="47701" checksum="9F95274D84A41B6D" modified="2023-11-08" version="1">MQAQGWVILLPGYDACIKPMDLTPIKIRGKKRKHTGLLDANHQSSKVPSQASSQASSQDQRPRKRKVKRKLVISAMPSLQGLPQELLEIIFLYSMNISLPRCSPSLGRKLSSRHVTMEFTMRSFFHTVDHRVINRDRKKTSDPQLQSEILSCRFFTYDFFLAYVRRAHDAWITLRGKAWEKTGVPVLGVKEFEGLWVFRFTKITYLSFAEGFHIPEKLLHGPFTKDKASLLYVLVSLNGEIDWTGSMAGETAKTGIKEAIKENNEYVVAALAVLLGVPKAITTDILRYAVINCGCNISVLRHLLFNAQILGGEVSKDTLDFYDPKLWAWTDTHGERGDVLKDMLKKADAFDLEFYFDEDVDWTKIVGFPYGGSRFDIRTAFDDLVRELLSNLYRNYGRKITRRRQRVSEHRIEEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM45</accession>
    <name>A0A9W9CM45_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_005001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM45"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19071">
        <property type="entry name" value="AKR_AKR1-5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000015">
        <property type="entry name" value="Oxidoreductase, aldo/keto reductase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF13">
        <property type="entry name" value="ALDO_KETO REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="6">
        <location>
            <begin position="27"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="5">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4370481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="281" mass="31474" checksum="985ACD2DCB10F565" modified="2023-11-08" version="1">MSLSIESSFPLANSKSKIPQIGFGVYQSPPETCVKSCLKALEAGYRHIDTAQYYANETEVGHAREQSKLPRSELYLTSKILSPAEDVESTYKKIAESVEKLDGPDGYADLFLVHSPNAGSEARRLMWQALELAKERNNVRDIGVSNYGIQHIEEIKSIGKTWPPVVNQVELHPWCQQREVVKYCKKNNIVVEAYCPLVRNEKAKDKTLLNISEKHNVGPNQILVRWSLQKGYVPLPKSDTPSRIVSNADVYGFELDEEDMAKLDGLDQGKKGAIVQAVTNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM60</accession>
    <name>A0A9W9CM60_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005525</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM60"/>
    <dbReference type="OrthoDB" id="10004862at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025337">
        <property type="entry name" value="Questin_oxidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35870:SF7">
        <property type="entry name" value="BAEYER-VILLIGER OXIDASE MDPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35870">
        <property type="entry name" value="PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G03330)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14027">
        <property type="entry name" value="Questin_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="2">
        <location>
            <begin position="68"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="800" mass="89166" checksum="10EE719814289104" modified="2023-11-08" version="1">MSTTTTTTIAKSQEAETPFLTGARKSGSSVQFWKSYVEARPSPTEDFFDLIYDYHQSHGDSRTDIAHDVGTGPGNIAARLATHFKHVVGSDINETVLEAAPYILPDETLKRMTFVHSPAEALAQKTPAQVGGQGTTDLVTVSECMPLLDETTALKAFHNLLRPGGTLGIYFYGPALFVDGDASKCNEIYDKIATMICTFNQPMKDTPGFPFHLRAAEALISDMDSVPMPTEDWESVERHKWNTEHSLLFNSKAGFDFDFSRVDRRTKEEVTKESSNPNFWGSEWSIENVKSFLDSLYPNYRDKAGARYAELENMFGELHAAMAGEKRKVTFPVVLILATKKDSPKLPKVNKVRISPEHAGIGRISESSPESFEMANQLLQKNHDEFHVFFRDLNGHNHLAHTILTTFALSGSPSELQRAYDDHVDIQRPMPELDTKVVKGLSEDKNMRAILGQPSHYTNALNFFRQQIESFGWRAVLHKFCFSRTPLADEVLSHMFEGAHHPFIHLGLAVEFELPSILAEALAQAVTHLNGRIGECLMQSDVEARPLSSGIPHKPILDLYKEARATESILHGARWEDGPFKMRDGTLGRSRADITSLAAQYRVDPCELELRAAEVINCSVYIAGASQRANLPPKIDFFYLHNVTSSIFLTVLVQDPWISLENKVRLVEWKARTDLFWYAACGCPEFDIQHVNNYQAGPSSGMGWSELYQAINVIHDDGHVAKFVRALKSAKDICKPFESQDPAAFQIKGDAWIQLARMAYDTTLDLALEVKWVPGTGFEQAWAAVSTKAPAPTAAAAAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMF9</accession>
    <name>A0A9W9CMF9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4370046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMF9"/>
    <dbReference type="OrthoDB" id="2135488at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052358">
        <property type="entry name" value="Aro_Compnd_Degr_Hydrolases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35563">
        <property type="entry name" value="BARREL METAL-DEPENDENT HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G16240)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35563:SF2">
        <property type="entry name" value="BARREL METAL-DEPENDENT HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G16240)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="1">
        <location>
            <begin position="16"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="154" mass="17765" checksum="96BEB8130899F37A" modified="2023-11-08" version="1">MRAWADMAPIIRKMDPRIKLVADHFGGTFPGEEKTPEFQTFLELIREKRLFVKVSGFERLYEGHGVGRVGMEAIKPIVAAVVEAGPDQIVYGSDWPHTQLGVTRKGKTDQQRLDDIEGFREVPDDVHIEVLREWIKDDQTWQKLFVSNSEKLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMK9</accession>
    <name>A0A9W9CMK9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_004555</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="303614at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000023">
        <property type="entry name" value="Sensor histidine kinase/response regulator, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.40:FF:000083">
        <property type="entry name" value="Sensor histidine kinase/response regulator, putative (AFU_orthologue AFUA_4G00660)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF11">
        <property type="entry name" value="HISTIDINE KINASE_RESPONSE REGULATOR, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="602"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="1099"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="42"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="318"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="663"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="320"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="664"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="1159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4371338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1311" mass="143898" checksum="E398C936364754CB" modified="2023-11-08" version="1">MADPHNAATARLHAGRSKKKMEHQLAVKAREFYLYYPQHPKSDGPLSCQPSPSPSPAPEPVAQTPPGSPLPSIDDAPRPSEDKALTAFAQLGALRLNARRCLISFFDRRDCYILAEATKSLSLYSGEPEFQEDCICWGSTIFPKEQSICYYTVNLPPQLAGLALDRHNDLPSLVVNDLTKDDRFKKYPFVVGPPYSRFYAGVPIRSPSGHSIGTYCVLDDKPRDGISDHELDFLKDMSGTVMRHLEMTRATQDQRRGKIMVKSLGSFAEGKSELENWWQDPWDSVIEQLPADRLQDTIVTRRQRAPVLPNVAEEPLVLNRNDSAESNIPSLTSKKSPDTTSISSGHPASTTATSAPDVVPEIQRPEVTTMISSTSTTSGEKDRIAPEVRAAFTRAANMIVDATEAEGVVFFDAKISTFGGLVDDDFFSDQLPEPDKPCIILGASLSKSPRGFKASADAEASMSESVLRHFLRLYPHGQIFNLDDDATSPAVNSPSAGGELEFSDGFPFTVSRKSESTRSLDDEAYLRTIFPKARSLVLYPLWDPHRDRWFASAVIWSSDPMRVFTSEQELSYLAAFSNSVMAEVARLDTKLADAAKADFISSISHELRSPLHGILGMTDLLKDTAIDNQQVSHVATIETCGKTLLETINHVLDFAKINNLTRGTSRRHTKRTQSAKHTINPGQAHTNDIMTLINDVDMSVLAEDVVESVFAGYTYAKTSAQTYEVRTPHADNPSISVILDINHSDNYIFRTQPGAWRRVIMNLFGNALKYTPSGYIRVKLEVKRKPGVADEGCEFRFTVTDSGIGMSEDYINNRLFHSFAQENPLSQGTGLGLSIVKQIVESLGGDVEVRSEKGRGTKFTVSCPLKESSMSPTFLASGPEQDKEKHFREVSKLTKGMKVRFVGFDEVDEYFVEGLTNKTAAKLAQKALDSLCTEWFGMERCTDGQEPDLVIATEACAKILRTQHNKNPEQTSAAPVIVVCQGAASAQSITAITVPGIIFECIGQPVGPHKMTKALSFCLERHRDRLPLQDTETDSTISMMSQLNLKENIVPSPRTDKPTPVLEASRPFLASVSSAPQIQSIGSSPLKQSIYSKPSRSLNCLCVDDNPINLRLLRTFVDKLGHRHALAINGLEALEHYKSVHSSPSSIDTVTAIDVVLMDINMPVMDGLEATRQIRAHEIRNGLPKVTIIALTGVAGADIQQEANSSGINLFLIKPVRLAELEIVLKGVATGQDKAKLELEHEKELEREREKAELALMSLHAGAGADADGHGAADDEKKELVSVGVKALEEVEQVQKVVTVTEVQGEEEAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMM3</accession>
    <name>A0A9W9CMM3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PRE3</name>
        <name evidence="11" type="ORF">N0V83_005050</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMM3"/>
    <dbReference type="OrthoDB" id="7854943at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03762">
        <property type="entry name" value="proteasome_beta_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000010">
        <property type="entry name" value="Proteasome subunit beta type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6 10 11" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4370529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="224" mass="24268" checksum="CCC2B76B5702990E" modified="2023-11-08" version="1">MMMSTGMLGEDGIHVDMNHLKKGEVNMAINFKDGVILAADSRTTTGAYIANRVTDKLTQVHDTIWCCRSGSAADTQAVADIVHYHLGMYGTTNDEPPTTQTAAAIFQELCYSNKDQLSAGLIIAGWDHRHGGQVYSIPLGGSLHKQSYAIGGSGSTYIYGYCDANWRENMTEEEGIKFVKGALSEAIKWDGSSGGVIRMVVLTGKGAQRHLYLPDQNYDGPGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN01</accession>
    <name>A0A9W9CN01_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">N0V83_004111</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN01"/>
    <dbReference type="OrthoDB" id="544685at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000345">
        <property type="entry name" value="Mechanosensitive ion channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.60:FF:000006">
        <property type="entry name" value="Predicted mechanosensitive ion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058650">
        <property type="entry name" value="Msy1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31323:SF15">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN MSY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31323">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN MSY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25886">
        <property type="entry name" value="Msy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="173"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="516"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="460"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="748"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="917"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="840"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="862"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="874"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="892"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="947"/>
            <end position="958"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4372337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="981" mass="110275" checksum="1B32CA4813762FB8" modified="2023-11-08" version="1">MTTPVTERPEPTYNMDGHTHVHQQRSSDDDGTFIGEPLSPHHSNTQENAEARSSNRPRSGTHGTHLTVETAHDMGPMRSMQSPSQTREQQSRLNDDLAVLQIEQELSNQHESLQRGHSVNRSMTRVRSRREDLVDEFDAATNPLHEQAARYKPPENPTTSFSKFFKKVHNSSFLIRYFTYITPMVILILIPLLVGALAFPNANVGGVKLVWFSIWLLIVWLTLWAGRLLAKMLPWPIGLVGSLFTNNSKKWRDMGKQLELPATLFFWWLAIEVSFLPTMTNHNINGDKSTQHWQKTMNKVLVTFFVGFVLNFVEKIIIQLIAISFHLRTYQDRIELNKFQIGSLAKLYKYSKEKIAMEDSEFEQYDGSGIPSGARTPGQLVTEAQKNIKVGFNKFGDIAGKVAGDFTGRQVNSSGHPNQVVLQLIGSTSGAQVLARRLYRTFAREETETVHSDDLKNAFESDEEADAAFSMFDKDMNGDISMEELEAVCVEIGRERKSITASLKDLDSVVSKLDDVFMFIVLIITIIVFISLISTSAAGVLTSAGSTLLALSWLFSATAQEFLQSVIFVFIKHPYDVGDRVMVYGNTGDLGKGDDYFVKEIALFYTEFKKMQGHIVQAPNSYLNTLFILNHRRSGALAEAVPIIIKFGTTLEQIERLRETLLAFVTAEKREYQTNILTELRAVQEVHWLEMNVVFFYKSNWQNELLRLQRRNKFICALTMALQECGIEGPRMRYPGQKESFPVYLQNLQNPATPGAGINGSPDNPSGNLRDTHQAQDEPFVAPLDGPSDTQGGSVPGRKTSILRQPGRPRGESLAAMGRRVDFSLGMKSMGLDDPSGDVLDDRENESRARAAVVRITSPSRSQERARESHDSGRSTSIQRVGTNSSSIARERTHRNRFFGRHRNADEEVAMADIPEADTPPAKQKLDPRTGLISPRAMRMSNEESRPPQSSGALPNTADSERTFMHPSRAQTDNIEMRRFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN06</accession>
    <name>A0A9W9CN06_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">ER membrane protein complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_003869</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The EMC seems to be required for efficient folding of proteins in the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN06"/>
    <dbReference type="OrthoDB" id="6745403at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008568">
        <property type="entry name" value="EMC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13116">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13116:SF5">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010045">
        <property type="entry name" value="DUF850_TM_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR010045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4372096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="260" mass="29148" checksum="AFCDD4CB4F9EBDC0" modified="2023-11-08" version="1">MGQPVVQTIHRDPALFWWILLPITVVMILTGILRHYAMTLLQTPPKKADLPKIRQQRSLVRGVNLRSHANVLSPSSFAARKAYMVQAFQEGKFLAEPEQRGKPRPNPMSDPAAMEGMMGMMKGNMAMMIPQTLIMGWINAFFSGFVIMKLPFPLTPQFKSMLQSGVGTRDLDVRWVSSLSWYFLTLFGLQPVYNFILGSNNAANQVTQQMAMANPGAGGVMGPEQDPDKLFLNEAENLEVLEHRWILEGIEDRLVAKFAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN85</accession>
    <name>A0A9W9CN85_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LEU5</name>
        <name evidence="16" type="ORF">N0V83_003145</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN85"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002167">
        <property type="entry name" value="GDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00928">
        <property type="entry name" value="GRAVESDC"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="352"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="51"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="150"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="293"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4372854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="384" mass="42130" checksum="04A7654C1A031F34" modified="2023-11-08" version="1">MTTTAAHANSADSINKSSQSVLRSTNHDANLPLKKDMDDGRPKKLDKKSAEYLIKSGLAGGFAGCAAKTVVGPLDRVKILFQTHNPQFAKYTGSWSGFPTAIRDIYQSAGIAGLFKGHSATLLRIFPYAGIKFLAYEQIRSRVIKNKAQETPSRRFISGSLAGMMSVFLTYPLEVIRVRLAFETRVEHKSSLSSIVRKIYAEQPPPISHNPSNVMTATATHVVEKVTPRSGLPNFLRGFTPTLLGMIPYAGASFLAHDSVSDLLRVPVLAPYTTLPHTSREESTTTSHKPAQLRYWAELTAGGSAGFFSQTVSYPLEVIRRRMQVGGVVGDGHRLGMAEVARRIYFEKGLRGFFVGLTIGYVKVVPMAAVSFYAYERGKYYLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNE0</accession>
    <name>A0A9W9CNE0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_004861</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-binding monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNE0"/>
    <dbReference type="OrthoDB" id="74360at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051209">
        <property type="entry name" value="FAD-bind_Monooxygenase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42877:SF4">
        <property type="entry name" value="FAD_NAD(P)-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42877">
        <property type="entry name" value="L-ORNITHINE N(5)-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4371641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="646" mass="74288" checksum="B0213C928EDC8530" modified="2023-11-08" version="1">MAWNGNAPPSENGTKQVDDAFIRTAIDKSNFNALRLALLQVTGDPDLAKMRTRRHAIRGGAMFAHVLAEEEVPNLKEKAFAYLSKRKPEDRIPPPPSKSKARKLMDIFGDEPVQEAEFEYNYEELAYNEFPRDAEWTTKKPSAETIDKFKVIVIGGGISGLAAAVQFKRLGFNFKVLERQGGIGGTWLLNSYPEARVDTSSFLFQFKFVKNYPWTEYFASAGETRQYLETVAERYQVKDNFYFNREVVSAVWHEDTSEWELTVQHADGHVENMRCNAIVSGSGLFSTPNKEPSIKEYKNYKGHTFHTAKWDHSIDYRDKRIALLGTGSTGTQLAPALARAGKSLTVYQRTPNWIMNMDGYRDRIDKYARYVFDNMPYYWNWYCYSSHVTSQQLQHLQPYDREWQAKGGIINERNDKVREALTDYIQSKAKGVPGLLEKILPKHAPLVRRLVVDNGFYDMLQRDNVELVTDTIETFTETGIRTADGKEREFDIVVLGTGFKTSQYFWPCKYVGRDGATMEELWKKDGARSYLGLVVPGFPNFFSFYGPNHQPRSGGFYSWGEIWARYTATCIIHLIENGKSWMDCRKEIYDEYNAKLDAECKGLIWEEAGHGYYVNEWGRQGVNVPWKTAEYHGMVSKPNLKDFDIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP25</accession>
    <name>A0A9W9CP25_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Succinate semialdehyde dehydrogenase NADP+ linked</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">UGA2_2</name>
        <name evidence="4" type="ORF">N0V83_002840</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP25"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050740">
        <property type="entry name" value="Aldehyde_DH_Superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353:SF5">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="3">
        <location>
            <begin position="60"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="140"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="161"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4374101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="176" mass="19775" checksum="B4CB94EDF07655DE" modified="2023-11-08" version="1">MRSFATATRSLARQRACRSSFQARQLPLIVSRGYANMGHSVPPLSDKSLLKEQTYVNGEWISAKSGKTFEVHDPATGSLIGTMPEMDSADTEAAIAAAAAALPSFRKLTGRERSRMLRKWWQLMMDNADDLAKLITWENGKSHSPTRKGRSTTQRAFSSGSRKKRRGRMVRLFRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPL2</accession>
    <name>A0A9W9CPL2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001963</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPL2"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="196" mass="20784" checksum="A67C26CEC0505BC2" modified="2023-11-08" version="1">MVEEEEEEELDGELEGVGVGVGVGVGLGEELDELEGVGLGDELDELDELELELELELELELDDDETSVGSGMNIDELELELELELELEVDDDETTVGSGTEIDELELELELTLDDDTAAELGSAVMVVVKNVTIAGAAEVLELDEDVAAGAAEVLELDEDVAAAAVEVDEEVLEVAEAAGAVVEDELDEAAWRATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ23</accession>
    <name>A0A9W9CQ23_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bifunctional AP-4-A phosphorylase/ADP sulfurylase</fullName>
            <ecNumber evidence="3">2.7.7.53</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">APA2</name>
        <name evidence="3" type="ORF">N0V83_003008</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.53" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ23"/>
    <dbReference type="OrthoDB" id="10267950at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003877">
        <property type="term" value="F:ATP:ADP adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009163">
        <property type="entry name" value="Ap4A_phos1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043171">
        <property type="entry name" value="Ap4A_phos1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045759">
        <property type="entry name" value="Ap4A_phos1/2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019200">
        <property type="entry name" value="ATP_adenylylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38420:SF3">
        <property type="entry name" value="5',5'''-P-1,P-4-TETRAPHOSPHATE PHOSPHORYLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38420">
        <property type="entry name" value="AP-4-A PHOSPHORYLASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19327">
        <property type="entry name" value="Ap4A_phos_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09830">
        <property type="entry name" value="ATP_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ap4A phosphorylase 1/2 N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="ATP adenylyltransferase C-terminal" evidence="1">
        <location>
            <begin position="207"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="348" mass="37927" checksum="1B01419A1C56B127" modified="2023-11-08" version="1">MLLGLSEALPHLVDAKFKAAKAASSLLFSPTELAIIRTSAGVPFQLRYCPALAKKPVPKQADATPKQKIDPFDNPPAALHIADVPTSNPTHLLVLNKFPIIAGHFILATKSNKKQTHVLEQDDLEATYACLKAWQEGSGGKQKRSFAFFNSGEHSGASQPHRHLQFLPVENMRDGEAASGWDLLIDLVLSGTKAQAKDAPSGLLQHPDIPFTHFARSFESEPSGRQLLSIYTELYSAAKETVDKFIAANPEQLALHPIIGGDLPISYNLAITTAGMVILPRRAEGTMLRRDDGSEVGFIALNGTTLGGTMMVKHQEEWDLLREKPEMLDHILAAIGIPREAPRSKSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ44</accession>
    <name>A0A9W9CQ44_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Meiotically up-regulated protein 86 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mug86</name>
        <name evidence="8" type="ORF">N0V83_003068</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ44"/>
    <dbReference type="OrthoDB" id="3648309at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015123">
        <property type="term" value="F:acetate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051633">
        <property type="entry name" value="AceTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047622">
        <property type="entry name" value="GPR1_FUN34_YAAH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038013">
        <property type="entry name" value="AceTr_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123:SF1">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01184">
        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01114">
        <property type="entry name" value="GPR1_FUN34_YAAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4374327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="300" mass="32344" checksum="9C272010DF88C161" modified="2023-11-08" version="1">MATAREHTDVARGPSFEKGTNGTSALDHAHNGNHTKQRAPYDYGGNPLAHVATNDPDMRLRAFGGEFQPGLYRAPNRKFANPAPLGLSAFALTTFVLSLINVNTRNIGHPNLVVALAYGYGGLVQLLAGMWEMAVGNTFGATALSSYGGFWLSFAIILTPGGFAIETDLVDKYGAPTFYNSFGLYLMGWFIFTFLLLLCTLRSTVAFFFLFFTLDMAFLLLGIGYLQHDAEGPQSGCIVAGGAFGLLAAFAAWYNALAGIADSSNSFFVVPVAHFPWSDKGRERRDKVDNSVARDAAHTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQG0</accession>
    <name>A0A9W9CQG0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pcc1-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001472</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTAG/PCC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQG0"/>
    <dbReference type="OrthoDB" id="10025739at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015419">
        <property type="entry name" value="CTAG/Pcc1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09341">
        <property type="entry name" value="Pcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="12523" checksum="174C55A553CDD146" modified="2023-11-08" version="1">MAARKEWKDEDFPCAFTAQRALSVDAELSPLVRRSFALTTPDTPSTQTDSTPSEDSVLQVNYAATTNRMLRVAVNGFIESVGVVIGVMKDLDVDVVYEPVTEGLQGVQGLDGTKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQI5</accession>
    <name>A0A9W9CQI5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3431 domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001564</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQI5"/>
    <dbReference type="OrthoDB" id="426718at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021838">
        <property type="entry name" value="DUF3431"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37490:SF3">
        <property type="entry name" value="DUF3431 DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37490">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11913">
        <property type="entry name" value="DUF3431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4376281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="39562" checksum="233EFE8CF686EFA9" modified="2023-11-08" version="1">MPRFTVRNAVVALNIVLLTALFVHLKYELWHKHAPAAELAGPLHLPDELLDDYQASTTPQADDGKTLKANAGITPRRTAVVVASQASENATWLSQYFPLWEKNIYHVDDANAPLTVPKNKGRESMVYLTYIIDHYDDLPDNVLFIHPNRYQWHNDDPDYDGLPMLRHFQLPYLEKEGYVNIRCAWSLGCPSEIKPLQEEGEHRQAVHAGGDYKKAFQHLFPELDVPKTVGVSCCAQFAATREKIRERKKSDYIRYRDWIIATKLDDNISGRVLEYSWHMIFGKKPVHCPHAEDCYCKVFGLCKLDCKDEGSCNGRYTLPPYSSLPKGWPYIGWQGEERQRAASEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRF2</accession>
    <name>A0A9W9CRF2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Elongation factor 1 alpha-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_000775</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the Dom34-Hbs1 complex, also named Pelota-HBS1L complex, composed of dom34 and hbs1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRF2"/>
    <dbReference type="OrthoDB" id="342024at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990533">
        <property type="term" value="C:Dom34-Hbs1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002184">
        <property type="term" value="P:cytoplasmic translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16267">
        <property type="entry name" value="HBS1-like_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000020">
        <property type="entry name" value="Translation elongation factor EF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000204">
        <property type="entry name" value="Translation elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015033">
        <property type="entry name" value="HBS1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08938">
        <property type="entry name" value="HBS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Tr-type G" evidence="13">
        <location>
            <begin position="405"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="237"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="251"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4377945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="828" mass="90938" checksum="5F14115FEE3B742C" modified="2023-11-08" version="1">MPPKSGFSRARNVDYDDDDIYDDEDEYYEEGEGGDGMTEDDKEQMRLGTTRVREGLGEMSGFTSDEQIQEALWHYYYDVDKSVVYLKNKLGTTESVEEKPKKEKTTSRFDQAASVADQKAPATTGKQIYAQYSCSGESAYLVTQRPLPPMSMPTSDTTDDFFWDVPWGQVPSDRLGVITTHASPYKGGLLGGSSKLAALAAKRRKEREEAEAAASKASSDADAAIAMLDKLTVRSKDNVPSSLGLNEQRPARSRYPVRKRSPSRSPPQEAPREAIEEPEAPQPAIINELPAQRATASMFASTLCGDCSSATSAQSAPALQAFPAPYAKYKDYDSAAAFATPSPDDIVRAAQAKGAGGGRRSTPKKKKAHTENLARSVEKLVVHDEPKIRSKNLNVVAEYEKSDLKRIANFVVVGHVDHGKSTLMGRLLYDLKVIDQRSIDKLRKEAETIGKSSFALAWVMDETNEERSRGVTVDIATNYFETEKSRFTILDAPGHKDFIPNMISGSSQADFPVLVIDASTNSFEAGLKGQTKEHILIARSMGMQHIIVAVNKMDTVSWSKARFDDISKRMTAFLTESSFLPSRITFIPLAGLTGENVVKKVENSAAHWYTGETLLEALERIELPQRNLQKALRLSVADVFRGDLRSPLSISGRIDSGTVQVGDVILALPAKQTATIKMIEVQDAPADWAVAGQIPTLHLTDIDPVHLQQGDIVCVPSAPVKLVKAFTSKLLAFQHVLPEPVEVFRSTLNSPGAIRTLSARLNKFTGEVVKKKPRVIKPGEVARVVVQLERELPIEEGMRVVLREGAGRWGRADGACCVSVDCRYERLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRU8</accession>
    <name>A0A9W9CRU8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000278</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRU8"/>
    <dbReference type="OrthoDB" id="5339332at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="500" mass="51628" checksum="4E57084B929FCA45" modified="2023-11-08" version="1">MPDSAANSNALSDPSQTPAAPPFSAPPTSHHHHHHASAAPAIADSGALRPALTPATTTRQSVALGLGSSLWEGNITPTLNAQPILSDSSVPAILTHAPSLDAQGAPSDLTASTGSVPSTTTTLTNTLPPTQSAALRALNAPAFKHSSPAKSKSAKSTSSRTTVTSQPVVVRTYSASRHASRPGSGFNTPRSFAMNGHTNASGLSAGLAGRSEQLPAADDFSFSAILRAVDPEIKDAIDAIAEICARSRMSLADEYDAHLPPQGEITGAGPGWVASTGALAGRGRLSRVSQGWTAADNTLMAVPEASSSSERLAQENKIGSSKRRSQSAYGSLKSVISGGSGKRKALDGDTFRDQEGSSSRQMDAQKQQGPAWTVHAPSSTHPSITLVTSPTASKQLSLDTSTALPDVPEEAGEASSARPQTALPQRAYGHRRGTSTLSLPASRSRSGTLSSIASWLPWPRPADFNPDSQQELTKAEARLREMLLSSSQSLGKGKSPVSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRZ1</accession>
    <name>A0A9W9CRZ1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vacuolar protein sorting-associated protein 54 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_000397</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRZ1"/>
    <dbReference type="OrthoDB" id="10259024at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039745">
        <property type="entry name" value="Vps54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012501">
        <property type="entry name" value="Vps54_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12965">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12965:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07928">
        <property type="entry name" value="Vps54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 54 C-terminal" evidence="9">
        <location>
            <begin position="838"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="153"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="557"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="811"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1086"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="319"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="563"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="820"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1099"/>
            <end position="1118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4377572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1118" mass="122742" checksum="22B30D7599A3DA47" modified="2023-11-08" version="1">MSSPRSPRRSEESFEFLSPNPQQQASFPFPSQHDWTNRPSSTGGRYQPRRGSTASSIHSVGGMLDSGFKSSMGAVREQNHNAISTLLQPPIVRTGMVPHTTASALSGHRPPSTKDIPPVTLTNIPHIEPSAFNPYLSQVGSLYEAFQRAKAEAEAETESAQPVRRGSKKEHISESLERSLHTASPLGTPNLSGAATSPAASPRPKRRTSGSKRALPAVTPLSTIPNVYFDENFHLENPRTFDIVSERSEVVRPVRTASNDNANIANGSLDAPQPTGRKALATNAILQEKLSWYMDTVEVHLISAISTASSSFFAALGSLRELQSEASESVERIKGLRADLRRLDEQMAVGGLKVVEMKRRRENLRKLADAVDQLQAVIVGLSHCDEVVNKGDLEIAMTRLDTIERLITGALDTSDSANVSWLDTRLPPQLIDLRSLRALDGVFEGITGLKFRVGRGFETRFVNTLLMDLREHVKRVPSSHTLERWVAASQKARGNHSRAKSIMPAYLTTSDRLRSDLRASLYGLSGAQFTNQASATFREAMVKEMKILIRKYLPSSTDDDTESMASVSTRGSRGHSQQDKNSILARNLRAMDPADAEEFFVSIFTDIGEALRRLGTQVKVLLDVTSGVSTPPASAGGLRSPPRSPYMNNMDSYLGNNTPAPASNDLQMELMQALDMSSLLGQAVDAAQTQITKLLKVRSEQTANLPLDRFLRYFGLCRLFADECEAVSGRSGAALKGVVNTHINDFVSRFGDFEKQELAKAMDSDRWEPKDFDAGDNDVLARILKGMESDPPTWAETGDVLREIKEPIANGASAHTNGTSEDKPKEKSKNTAPAIVDEEKYTISYSSTVVIRGIERFEILLSAIPSITSEVSISLCEYIKLFNSRLCQLILGAGAMQTAGLKNINTKHLAIASQALSFIIVILPYIRECARRRIATATNKSAIGEFDNVKRLLHDQQASIHEKLTDILSGRATVHMRSLKKVEWDTDAEINKDVSSSMESLTKDTVTMHKVINKYLSEMQVRMIMAPVFESYRDQVGKVVKEAVVKTPGGKARLLREAKLFDAKLGPIDGAGNVGSYLIQLVDGKQVAQPKAEPEPEPDTEKKEAVEPEAENRKQDTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CS13</accession>
    <name>A0A9W9CS13_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CS13"/>
    <dbReference type="OrthoDB" id="5386330at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37540">
        <property type="entry name" value="TRANSCRIPTION FACTOR (ACR-2), PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37540:SF5">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="462" mass="51644" checksum="FB354C8EE8A69525" modified="2023-11-08" version="1">MLAQQTKGPESNGDNGGRSLPPSRKEKRPKTFMFIDSSNGGINAKPDRVVRSFVMKSARNKKPWSTRPRNPKTDTLTIVEATTPRLLPIDPHRSSEVAATDWVAACIQSPIGAPFIYAVLTSSARATSLDSEVYKWRAIAEVNRLLTDSHKNTDDTTIATVLMLLALEEADLADPRKKGDERRCSVRVKEAHLNGLKTMIGQRGGLAALGQNRYPGLFDIVSTITVFISDLTAWYETGICPVDSFELQKHASLLMYRLFDWHEQHTNNQNEETRQVDESICLALLIFIVNATEPNAFSFGSRLSKTVNKLHLALERVPLHHWSHAPDMLLWTLTMGALGAKTLPRGSRASTSTGPELSFFMQLSPAAVSDRSFKLASMNLQLLLDKLKSCLWMPSIFDERLKRLWVSMGLCKPEVVDVEDTSSSSEGEREQLVDDEYALGQSTTMRFFKLEGKKRVKREPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSH0</accession>
    <name>A0A9W9CSH0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000961</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSH0"/>
    <dbReference type="OrthoDB" id="4822at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47251">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04180)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47251:SF1">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04180)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4378128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="203" mass="22304" checksum="C5F2126D14C0B6AF" modified="2023-11-08" version="1">MPPQRTKPNPAAIKIFNCKTCDKGYARQIEYENHLSSYDHNHRQRMIDMKKTTDDYDKTSAKPKQGLEMRRIDVQAASKMPGVGFKKLGGASLASGPALASGPMFRKIGKAATAEVKKEDGAAKGDGVPTKSEEKTPGALAEAEKPVEVKKDEDIVMEDAVEDEAITWEEYDFTKPTGCDHANCPGRKTDGIWDDEKENVDTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSM5</accession>
    <name>A0A9W9CSM5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Alpha-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Maltase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_000962</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing (1-&gt;4)-linked alpha-D-glucose residues with release of alpha-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.20"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.20" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSM5"/>
    <dbReference type="OrthoDB" id="1740265at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004558">
        <property type="term" value="F:alpha-1,4-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004556">
        <property type="term" value="F:alpha-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033934">
        <property type="term" value="F:glucan 1,4-alpha-maltotriohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004574">
        <property type="term" value="F:oligo-1,6-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004575">
        <property type="term" value="F:sucrose alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000025">
        <property type="term" value="P:maltose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005987">
        <property type="term" value="P:sucrose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11333">
        <property type="entry name" value="AmyAc_SI_OligoGlu_DGase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000064">
        <property type="entry name" value="Oligo-1,6-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000087">
        <property type="entry name" value="Oligo-1,6-glucosidase IMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.400.10:FF:000003">
        <property type="entry name" value="Probable alpha-glucosidase (Maltase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="entry name" value="Oligo-1,6-glucosidase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF222">
        <property type="entry name" value="MALTASE MALT (AFU_ORTHOLOGUE AFUA_8G07070)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0462">Maltose metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="9">
        <location>
            <begin position="22"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00026248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4378129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="587" mass="68279" checksum="845EBC7C02A2E71D" modified="2023-11-08" version="1">MSPHADPLHHKRVWWKEATFYQIYPASFKDSNGDGWGDIPGILQKIDYIASIGVDVVWLSPMFDSPQIDMGYDVSDYEAVYAPYGCVEDVEKLIEACHKNHMKLILDLVINHTSDQHAWFKESRGSKDNPKRDWYFWRPARYDKQGNRMPPTNYRSYFAGGTWTWDEHTQEYYLHLYDTTQPDLNWENEDCRQAIYNSAMRFWLDKGIDGFRIDTVNKYSKVLPLIDAPVTDPTNDIQPAAQMWCNGPRIHEFIKEINRDVLSKYRTYDGQPVVTVGELSLCSDAESVIPYVSALEKELDMVFQFDITHLGQGPPGCNDKYDFAEWKLPEMKRIVEKWQCFIEGTDAWTTVFNENHDNGRSISRYANDAPEWREKSAKMLAIWLIGQTGSLFLYQGQEIGMINIPKEWGMEEYKDVESQNYWAEAVRLAEDGTDPTRKERIGYGLQLMARDHSRLPFQWDGSINGGFSTGKPWMRAHDNYKSINAASQEKDADSVLSFYKRMLRLRKEHKDIFVYGTFELLDPDNLNTFVFRKRYQEKTALVVLNFTTTPQLMSGTEEMKLLVSSQSKKIPGTLQPFEGRIYINYEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XWZ6</accession>
    <name>A0A9W8XWZ6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010833</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XWZ6"/>
    <dbReference type="OrthoDB" id="10565428at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="569"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="569"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="604"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="629" mass="71208" checksum="1705CEDEAB9A7AF1" modified="2023-11-08" version="1">MSSQQLVCAPHRRLAWLVIEAKHVYEQFPAELSQQEWLKMKLHERDNLVMARTHSVASLSAALDKVPQAAHAQTFRTGCMDGKAKEMFAPDKDKLIGQIVEEGQEVLRQLRETQTLQSTQSPSVKKQYFGHGFYSMAVMSVPVGRLAPTGGPESTPDKTPIIYASLDSNGTVHFTASNKQNVKLTNVLFSQQAINLGWEKEIKYRRTAATVLAKAHVLAPPPASFPEIKVALEDQRHVPLLLMQLSRLGHLGKVNTTFVKEAQAEHDRLWARWTPKLNKFFAVIRGDERAFPSLLVRAAATLHSLAQLDATLTEILDFPGLDRWLWLTMREVVDLSTERPTFLLKDRAGWEEWVGGVTPWEVRADRDCKHVDVFQQSDLAGLRELCPIAANRPVELAMPAREVLGRGILACNFAPQASSGDIYRALTPTWTIHYQRAMEEMFGGREIPPIPTQPHIPQAPEQLVGSAPELVVCAVYNAVIHRYSDGRPEGSTYIVIQWFKELYQAFKSRDNDRFRELIRLHKRWEADGTLVVNEETTGLQGPDLDQRRFEYHAGLGEDVTNPSDYRWDRSEKRRRMDDKKMALSGKAPAKREKNVLYTDGQGNVHDKSGKHYEQQPVDEGDLDDFTRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYE7</accession>
    <name>A0A9W8XYE7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Porphobilinogen deaminase</fullName>
            <ecNumber evidence="5">2.5.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Hydroxymethylbilane synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Pre-uroporphyrinogen synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">HEM3</name>
        <name evidence="16" type="ORF">N0V83_010474</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="EC" id="2.5.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HMBS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYE7"/>
    <dbReference type="OrthoDB" id="564646at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000005">
        <property type="entry name" value="Porphobilinogen deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000086">
        <property type="entry name" value="Probable porphobilinogen deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.40">
        <property type="entry name" value="Porphobilinogen deaminase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022419">
        <property type="entry name" value="Porphobilin_deaminase_cofac_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022418">
        <property type="entry name" value="Porphobilinogen_deaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036803">
        <property type="entry name" value="Porphobilinogen_deaminase_C_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557:SF0">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03900">
        <property type="entry name" value="Porphobil_deamC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54782">
        <property type="entry name" value="Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00533">
        <property type="entry name" value="PORPHOBILINOGEN_DEAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="9" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Porphobilinogen deaminase N-terminal" evidence="14">
        <location>
            <begin position="31"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Porphobilinogen deaminase C-terminal" evidence="15">
        <location>
            <begin position="263"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4362381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="398" mass="43069" checksum="941F2B3B7DE3ACBB" modified="2023-11-08" version="1">MASATATDLQAPANPAPQAANTSTSTQFPPVNIGTRNSTLAQIQARAVQRALKDAHPDRTYNICPVVVQGDRDKITPLQQLSQGENAKSLWTGELESMLESGDLDIIVHCLKDMPTQLPEHLELGAILEREDPRDALLISPRLPKDTTLATLPQGATIGTSSVRRAATLRKLFPHLKFADLRGNVGTRLAKLDAEDSPYSAIILAAAGLKRMGLDSRITQYLSSTNGGMLHAVGQGALAIEIRKNDQSMKDILARVRCERTTRACSAERALLRTLEGGCSVPIGVETSWRGGKGLAIGAQPARDYDKHGNAVEEEEPDLEDQELVLRALVVSVDGTESVEYETARKIKSAEEAENMGREVAKVLIEKGADKILEKINVEKQWAAKKKLEELQSASSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYN1</accession>
    <name>A0A9W8XYN1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_010511</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYN1"/>
    <dbReference type="OrthoDB" id="5296287at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF68">
        <property type="entry name" value="MULTIDRUG TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01120)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="395"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="428"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="455"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="55"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4362418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="492" mass="54103" checksum="72CB9EC2A9E8343C" modified="2023-11-08" version="1">MDAKQVHVAADDAEKGPESSSTAARGGPISNIVDWDDLNDPDYPMNWSKSRKFKNISVICYCTFLTPLGSTMFAPAIQDVMRTYQSNDPLLASFVVSVWILGYFFGPLFLGPLSELYGRLPVYLVCNVLFTVFNICTAVSPSLAALVVFRFFSGTFGGCPITIGAGSFGDMIHPRSRGKIIAIWSLGPMIGPIVGPIAGGYLGASVGWRWICWTLAIASGAGAFASFIFQEETYPPTLIERKCKKLKKLTGNQALRTDVHMDRKPSHVFGRAIIRPLRLLFLSPIVSILSLYMGVVYGFMYLLFTTFPLVFQVQYGFGTGTIGLTYLGLGIGSLLGLGVAGYMSDRIYHKKAVDGRMEPEWRIVPLIPSAFLIPAGLFWYGWTTYYKTHWILPELGTVLIGIGINVLMMCISTYFIDAHPRFEASATAAATAVRSLIGALVPLFGRSMYEAMGLGWGNSLLGFITVVMAPLPFLFFKYGQRIRTNTRFQPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZC3</accession>
    <name>A0A9W8XZC3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_009944</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZC3"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001113">
        <property type="entry name" value="RNP domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="46"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="224"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="122"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="315"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="316"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="329"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4363648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="354" mass="37513" checksum="D7773FD104077E48" modified="2023-11-08" version="1">MSATDSVTAQMANTSLDNAPTTESTGAQQPTTAEDEAVRASAAEGRRLYIGNLAYATTEGELKDFFKDYLVESTTIPTNPRTSRPVGYAFVALSTPSEAERAIAELNGKAILDRKVSIQLARTPEAHAEGAGSGAEGTSSEHRRRTSTRGRGRGRGRGGRAGRGGRAADGAEGAEQVELTDAAPAESTPTNGTDDKKTSAPREPREPRERKQRGPPEDGVPSKTKVMVANLPYDLKEDKLLEIFADYSPTQAKIALRPIPRFMVKKLQARGEPRKGRGFGFVTLGSEELQQKACSEMNGKEIEGREIAVKVAIDSPGKEDDHIEGGEAEGAATEGATTESAEKATEAPAATTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZC5</accession>
    <name>A0A9W8XZC5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Cysteine dioxygenase</fullName>
            <ecNumber evidence="2 7">1.13.11.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010961</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteine + O2 = 3-sulfino-L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20441"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:61085"/>
            <dbReference type="EC" id="1.13.11.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="7">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the cysteine dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.20" evidence="2 7"/>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZC5"/>
    <dbReference type="OrthoDB" id="543511at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017172">
        <property type="term" value="F:cysteine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019448">
        <property type="term" value="P:L-cysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010300">
        <property type="entry name" value="CDO_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918">
        <property type="entry name" value="CYSTEINE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918:SF1">
        <property type="entry name" value="CYSTEINE DIOXYGENASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05995">
        <property type="entry name" value="CDO_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4362020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="159" mass="18301" checksum="03F9720E9A504E6A" modified="2023-11-08" version="1">MPGVTTEQDKFQTLVQTLREKLGPCSGIDSEDVDPRELQQLMEEYNSDESEWAKYYFPSSTIPYTRNLVDKGNGKSNLVLKGSLVETRFAWPTQHLNNSEDRPLQVISEKTYEENQVTYMSDKLGLHRIKNPNPNDYAISLHCWSTSPRNTLKYVAHNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZC7</accession>
    <name>A0A9W8XZC7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetamidase</fullName>
            <ecNumber evidence="4">3.5.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">AMD2</name>
        <name evidence="4" type="ORF">N0V83_009988</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.4" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZC7"/>
    <dbReference type="OrthoDB" id="3787804at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072">
        <property type="entry name" value="AMIDASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072:SF5">
        <property type="entry name" value="GENERAL AMIDASE-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="3">
        <location>
            <begin position="2"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4363691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="287" mass="31946" checksum="004C2890BCD92B0F" modified="2023-11-08" version="1">MEGPMATTVDAIELFTKTIVNAQPWLYDPNCTPLPWNQTALESVISARKLRIGVFEWDDICLPQPPIRNAIKNVTKALKAAGHEIIPWKIDNKRAVELVLRVFRADSAGDIQRQCAKSGEPPMESACDSPEPPKTLLESWDLAMECTDFRAAVLKKWNETAKEDSPPMDAYIAPVNPAVAPRHGDYSKVRYFAYTASVNILDYSSCTLPVGFVDPIVDLADDTTLVMDAAGAELPRPTCERDETIRRKYDPQTYAGMPVTLQVVGRKFEEEKVIGIVKVIKALLKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZD5</accession>
    <name>A0A9W8XZD5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EXPERA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010195</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZD5"/>
    <dbReference type="OrthoDB" id="258627at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd03112">
        <property type="entry name" value="CobW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1220.10">
        <property type="entry name" value="CobW-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036627">
        <property type="entry name" value="CobW-likC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033118">
        <property type="entry name" value="EXPERA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051316">
        <property type="entry name" value="Zinc-reg_GTPase_activator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748">
        <property type="entry name" value="COBW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748:SF31">
        <property type="entry name" value="ZINC-REGULATED GTPASE METALLOPROTEIN ACTIVATOR 1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05241">
        <property type="entry name" value="EBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90002">
        <property type="entry name" value="Hypothetical protein YjiA, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51751">
        <property type="entry name" value="EXPERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="441"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="474"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="514"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="EXPERA" evidence="7">
        <location>
            <begin position="387"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4363075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="551" mass="61162" checksum="A2937FC2CF6B6347" modified="2023-11-08" version="1">MDANASDDEVPSLVQPEVNAKVLNEQSEEVPDLVASRDDLPSSKVPITIITGYLGAGKTTLLNYILTEQHGKKIAVILNEFGDSIDIEKQLTVSDANSTSASPVPFVPLANGCICCSVKDIGVAAIENLMEQSGLFDYILLETTGLADPGNIAPMFWLDDGLGSTIFLDGIVTLVDAKNVLKSLDEGYGDEEKLEERQHEEHEQHGPLLTTAHLQISHADVIIINKTDLVSEEELERVVGRVRSINGLAKIKTTTKSQVPQLEGLVLDLHAYDEVTDADLKFASKGHSHLDPTITTTTISFPSLTRSQVDKFDLFLRTMLWEETLPNSTSHKPFEIHRLKARIPVEDGKMLLIQGVRNIYDTNESNAPIGAESPGAKLVLIGRERKRDLVYLVFFIVHLPVMLAFDLSALYPVQIKPLWMGTLREWYVATYADRFFHDPPAWFPTLTFLELVYHLPLTLWAIPALLRNDPRIPLALLVFGLETSLTTLVCLAEMLSWGELSAAQRGLEGLSGMYGGYLATGIFMTLDCYARLDQMISKQHRYLEPVTKKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZD6</accession>
    <name>A0A9W8XZD6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GDP/GTP exchange factor Sec2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_010408</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZD6"/>
    <dbReference type="OrthoDB" id="5560525at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070319">
        <property type="term" value="C:Golgi to plasma membrane transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040351">
        <property type="entry name" value="RAB3IL/RAB3IP/Sec2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009449">
        <property type="entry name" value="Sec2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14430:SF4">
        <property type="entry name" value="GDP_GTP EXCHANGE FACTOR SEC2 N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14430">
        <property type="entry name" value="RABIN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06428">
        <property type="entry name" value="Sec2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144284">
        <property type="entry name" value="Sec2 N-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GDP/GTP exchange factor Sec2 N-terminal" evidence="4">
        <location>
            <begin position="157"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="76"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="40"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="161"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="117"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="132"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4362315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="261" mass="29332" checksum="3278E6C600F7761D" modified="2023-11-08" version="1">MAAAVELPPPPPSIHTKHIANNVADCPTCGTSVPFNIEEFESARKRIIELESQMELLKEKATAAVDRCADYEDQIRSLRSVQSRDRLPRSNTSDSLDPSPPYDEQPRPATAGATTARQSRFSFLTARRTSPTTPPPPHPPPPPKDQYYPQPQPDIALLNELERERALRAKAEARVQKVDSEIEELSVQLFSQANEMVADERRARAKLEERVELLERRDREKMGRLERLEKAVSRIDRVKAMLKDEPVGTTNGGTLALPARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0C8</accession>
    <name>A0A9W8Y0C8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Iron-sulfur cluster biogenesis chaperone, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SSC1</name>
        <name evidence="13" type="ORF">N0V83_010242</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for the assembly of iron-sulfur (Fe/S) clusters in mitochondria. Assisted by the DnaJ-like co-chaperone jac1 and the nucleotide exchange factor mge1, it mediates ATP-dependent Fe-S cluster transfer from the scaffold proteins isu1/isu2 to grx5.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0C8"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11733">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000014">
        <property type="entry name" value="Chaperone protein DnaK HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000020">
        <property type="entry name" value="Chaperone protein HscA homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000003">
        <property type="entry name" value="Heat shock protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000007">
        <property type="entry name" value="Heat shock protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000004">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000003">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003520">
        <property type="entry name" value="PRK05183.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="613"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="573"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="652"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4363122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="673" mass="73183" checksum="37D7BEEA3A0CCED4" modified="2023-11-08" version="1">MLSSRLSRTFLPRAASAARRTPAFRAPAMFARGYAEGGEEKVKGSVIGIDLGTTNSAVALMEGKVPRIIENSEGGRTTPSVVGFTKEGERLVGIAAKRQAVVNPENTLFATKRLIGRKFTDAEVQRDIQQVPYKIVQHTNGDAWLEAQGQKYSPSQVGGFVLGKMKETAESYMGKNVKNAVVTVPAYFNDSQRQATKDAGQIAGLNVLRVVNEPTAAALAYGLDKATDNVVAVYDLGGGTFDISILEIQAGVFEVKSTNGDTHLGGEDFDITLVRHLVQQFKKEQGIDLNSDRMAIQRIREAAEKAKIELSSSSQTDINLPFITADASGPKHINTKLTRAQLEKMMDPLISRTVDPVRKALKDANLQAKDISEVILVGGMTRMPKVTESVKSIFGRDPAKSVNPDEAVAIGAAIQGAVLAGEVTDLLLLDVTPLSLGIETLGGVFTRLINRNTTIPTKKSQVFSTAADFQSAVEIKVYQGERELVRDNKLLGNFQLVGIPPAHRGVPQVEVTFDIDADSIVHVHAKDKSTNKDQSITIASGSGLSDSEIENMVRDSEQYAEQDKERKNAIEAANRADSVVNDTEKALKEFEDRLDKTEAEKIKEKITSMREFISQSQAGEGTATAAEIKEKTDELQNASLSLFDQMHKARSESQNQGEQQQQQQPEGEGEKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y187</accession>
    <name>A0A9W8Y187_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_008874</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; 4-aminobutanoate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y187"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07103">
        <property type="entry name" value="ALDH_F5_SSADH_GabD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000023">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000004">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050740">
        <property type="entry name" value="Aldehyde_DH_Superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353:SF7">
        <property type="entry name" value="SUCCINATE SEMIALDEHYDE DEHYDROGENASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="4">
        <location>
            <begin position="29"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4365255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="501" mass="54799" checksum="91CC684F5B6874CF" modified="2023-11-08" version="1">MGSTGKYELPFKLNNPDLLHNLSYVNGEWVKAKSGKTFEVVDPGTGKPWVECPTNDASDVDEAVKSSHASFLEYKKISPRQRAQMLYKWDALIKENREDIATLLVYETGKPKSEAYGEIDYSTGFTWWFAGEAERIQGTVFTPALPNRRIFTIKQPLGVTAALVPWNFPIAMILRKAGAALAAGCTMIIKPSPETPITVLTLAYLAEKAGFPKGALNVLTTDLDKTPELSEALCRHPLISKVTFTGSTRVGKLVAKICSDNLKKVTLELGGNCPVLIFDDANIEQAMGQIFALKYRHAGQACITANRIYVQSGIFDRFLERWNAETQKIVVGHGADEKTTMGPVTTPRSIKKALELVADAKKKGAKIHTGGNKVDKNGGYFFEPTVISGVTPDMDIAKEEVFSPICTFIKFETEDEAVKAANDTSMGLASYLFTKNVDRLWRLFESLEAGMIGLNTGNSSAAESPFGGIKQSGYGKESGKDVAVNEYLITKTGTFTLEEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1P3</accession>
    <name>A0A9W8Y1P3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009329</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1P3"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="127" mass="12716" checksum="A567E718CF797F40" modified="2023-11-08" version="1">MTLVSTMPAAYASSKSSKDANASPSVDTMTSGDGASAAASSKNKDGESFFQTSKSTGDKDGTRTEKTWGKRGGDEKDSKKASKELAAQNHTEADDDQKVFAKAGNAFAAAGGAVASAVGGKAVASAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y218</accession>
    <name>A0A9W8Y218_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinetochore complex Sim4 subunit Fta1-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y218"/>
    <dbReference type="OrthoDB" id="8864979at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025204">
        <property type="entry name" value="CENP-L"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13092">
        <property type="entry name" value="CENP-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="396" mass="43596" checksum="BF2C3153B18A4CCA" modified="2023-11-08" version="1">MADISPYPLYNRTYHLYRLSPLHHHGQPLLADTALSTHAKRLKEQLKGDNVRGVQVDFARTEDTAKLGPLEECNWDMIGDEDAWIDRHRQSVDPDASQLSTGLTADRARGIDVVLEYEKQSYNALLLRDPNVTASPEGFTSLPLLLVKMPGPIREIFLNYLRTSFDAHVAPLRLPPRFITSSLETYFSRLSANTSTQTIQDVVRQLHIQLSFPTATTLLKHIDITIAATDVPAFVSRGKLMKNAQDQPFTAALSAYLRHHLALDISQPRAQISKIACNSFHLSTERLKLSAPDPLADTSFSDEGASSQDASAGQLAVQDFLTSLVKEAAGSGTFLSEDLTTDRREETPSSSASVKAGRRKRAVSNAAVGNDNKKKAKARGKENGVRVDGDENMVHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2G2</accession>
    <name>A0A9W8Y2G2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Yeast cell wall synthesis Kre9/Knh1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009621</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2G2"/>
    <dbReference type="OrthoDB" id="4094614at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018466">
        <property type="entry name" value="Kre9/Knh1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052982">
        <property type="entry name" value="SRP1/TIP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40633:SF1">
        <property type="entry name" value="GPI ANCHORED SERINE-THREONINE RICH PROTEIN (AFU_ORTHOLOGUE AFUA_1G03630)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40633">
        <property type="entry name" value="MATRIX PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G05410)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10342">
        <property type="entry name" value="Kre9_KNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040817539" description="Yeast cell wall synthesis Kre9/Knh1-like N-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Yeast cell wall synthesis Kre9/Knh1-like N-terminal" evidence="3">
        <location>
            <begin position="36"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="244" mass="24513" checksum="A9486849ABD5EF25" modified="2023-11-08" version="1">MFSKAAIATFFASLAAAQLHAPVGEPSGNPITRPLNEVVPACEPFTITWQPTTPNTVSVLLLKGPSTNVVKFGPALAEGIANSGSLAWTPASDLEATDGPTGYGIQLIDDVTGQYQYSTQFGISKEGCVSVSSSASMASSTASGYPVSSPSASASKASAPYPISSAHYTTAAPPKSTLVYSTGMPHGNTSVIYPTGSMTVPSSLKPSATGNATTSRLPEATGAASALQAGLSLAGAVAAFAFML</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2K5</accession>
    <name>A0A9W8Y2K5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2K5"/>
    <dbReference type="OrthoDB" id="28755at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37490">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37490:SF1">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 2-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="421" mass="48155" checksum="1F2424E9D08555BE" modified="2023-11-08" version="1">MNTYSFDRSSRTAGTQSHRVIHDTLVLLALAGLVGCYSTLSLRRSFTTWYQLGCFILAVLCISSKAAHDGKESYSEQQRYTQEYHELVTDDSPIPSDNTEATASFQDISDAEARTPHTEPRGVSYRVFLVSIAMVVLWTAHLLLNFTEREGHHVNTILDFKYKPTRSLEVVLSMYKEPTEEVSQLISNLKGIPSLSEATYTIYLKDNEANEKEVKQATGADSVGLLPNVGREAETYLNHILSRWNSLAKQTIFLQADVHNQREFYPHLNQYFIPDRTGFLSLGWSGSVCNCDSCGDRFFWRDETHLFPAIQSRIDNSSECRNVLLSYKGQFIVSAKRIRGIDKAIYHDLWQAFVDEKSWAHQEDYLRGRPDSMSEPWFGYTMERMWNLLFQCNDMDVAWKCPTLLSGWRSGGDIGDCQCFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y307</accession>
    <name>A0A9W8Y307_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">HOG (High osmolarity glycerol) pathway protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">NBP2</name>
        <name evidence="5" type="ORF">N0V83_009608</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y307"/>
    <dbReference type="OrthoDB" id="19092at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="335"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="136"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="408"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="167"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="240"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="454"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="490"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4364153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="506" mass="55504" checksum="EA57E6226CBB8125" modified="2023-11-08" version="1">MTDTATAPETPAETPRLDDITEGVVVRGRQNRSDMAADVLSPRSEPGSAGTMGVNRTSLPPLVTSPPSRAKVERASSYINKRLSTFSNTSNTPQQRPSRPQSIAYPIFHSSLPYSLVRDFAYDPFHPLFYGPLPEQPSDISTPASEVGRRLSDPPPVPWRSDRGWSAGSNDENGEQLPQTSYDGGPPYSEDEDISSPVVTSHHGARHKKHKSNIVNFDQTRGRRGYPEEHRRGSIVPVNNEDTQAFNGTETIDSADGSRSEYITYPPNSSRLGVPEGASRRDSHFAATLPPRTYADAEGPPYDSDDDMSEPEDYIHDDNRFSRDYQFTIASPDEEMHGKAVALFDFARENDNELPLVEGQVILVSYRHGQGWLVAQDPKTGESGLVPEEYVRLLRDIEGGWNGLMNGTIKSQEANNDGPEGLLSPVSADPEAKTPTQADHPLNYTPVISTFSTSHKDLEPYPKDRLGTPTNPEGGSFGKGKESGAMEGVESTPEPQTQQQQQQQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y391</accession>
    <name>A0A9W8Y391_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4470 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008497</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y391"/>
    <dbReference type="OrthoDB" id="5282002at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027974">
        <property type="entry name" value="DUF4470"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14737">
        <property type="entry name" value="DUF4470"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4470" evidence="1">
        <location>
            <begin position="36"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="506" mass="57677" checksum="DAF92A59A201AE3E" modified="2023-11-08" version="1">MKDTRVPDWYNTNRQPAFIRHGAGPMNAPFGTPKYPWGNMPALNILQLKDNEGETEVNRDFKLLFAASGDLRNAIKSVDGLPHGYKGECVAVLNDKDLTIVARNAIMLMIALRFPPKAAVPMIIHLWYSALLPAVMVDTIQSDILPLFQDVCSKIEDKPQNSLQAKTFNFDGRKLRMVLKKQEWDQLVKFCQVPPGLTASEAQIIRRRIMLAPERVDFQDKVMLDLTKAGRQGEMYFRERGILLPHSCSTKDFDTPNPASPRGGWSYPDYMKNAPPATADEFGAIFYYIRNLLLRFCGIVQSSQISFHLLCVDATLLGDYPYVDDMKFDRIEISNICDGEFVGPPICIDRFSRLLKPRSENPHATLLMLFLNAAAATEYVHNSDSNAQETEVAMDRLAHYMPYDEASLLNAVLHTSRTGVEHPILVHRASCAAKLKDWVNYFDIFIRETNIPEFAQLCGLVIKEEHTIVQPWPYLVRLTTTRQEFEILMASERSGHERYIEFRRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y395</accession>
    <name>A0A9W8Y395_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit F</fullName>
            <shortName evidence="4">eIF3f</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_008591</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit F family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4364975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y395"/>
    <dbReference type="OrthoDB" id="25498at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08064">
        <property type="entry name" value="MPN_eIF3f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000019">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03005">
        <property type="entry name" value="eIF3f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027531">
        <property type="entry name" value="eIF3f"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024969">
        <property type="entry name" value="EIF3F/CSN6-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540:SF6">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13012">
        <property type="entry name" value="MitMem_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="6">
        <location>
            <begin position="30"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="312"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="317"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="339"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4364975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="404" mass="43314" checksum="75C3061DD78FA0DD" modified="2023-11-08" version="1">MPDNDSFLHLARPLGPAPIGAQPSTAPLNVAIQPQAVFSILDHSLRRPADQERVIGTLLGTRSEDGTEIEIRNCYAVPHTETAEQVEVDMDYQKQMLALHLRANPREVLVGWYATSSDLNTFSALIQNFYSQQGDGTWPHPAVHLTVSTVPGQEIESRTYISAPIGVTAERAADSCLFLPVPHEIKYGEAEKSGLELISSAKDREDRSQEILTDLDALERAIVHVLDMLERVSNYVNNVLDEEAEPSSALGQFLMNALSLAPKVDPADIERDFNAHIQDVLVVSYLANTIRTQIDLSNRLATAALTLGGGDALAADGQKDGGENRGKGGDRRGGGKGRSNSVSAQTLQAPEEVVDVAPEQAASSGVEETRAISNEAILNEGVASGVCVLSDQAHAILQASASKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3A6</accession>
    <name>A0A9W8Y3A6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_008606</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4364990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3A6"/>
    <dbReference type="OrthoDB" id="2962696at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052178">
        <property type="entry name" value="Sec_Metab_Biosynth_SDR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618">
        <property type="entry name" value="7-ALPHA-HYDROXYSTEROID DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618:SF1">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4364990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="31425" checksum="8B1837B4929BBDEE" modified="2023-11-08" version="1">MANTSDLRVEKLFSVKDYVCVVTGGGSGIGLMAAQALAANGAKVYITGRRKDVLENAAKTHSPSGGGQIIPLGTCDVTKKDDLERMYEELSSKEKYINLLVASAGISGERGEPDAEKATDMKKKLWDNETFEGWAETYNTDVTAVYFTTVCLLPLLQAGTESHGHLSASVIVISSMSGIMRDAQGHFSYNAAKGGTVHLTKLMSGEFQKLRIRVNSIAPGYFPSEMTAKESDENQKSHVPDEKIQDKGHVPMQRAGSDQEMAQAVLFLTKNSYVNGEIIAVDGGVLNVVAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3G8</accession>
    <name>A0A9W8Y3G8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane nucleoporin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">POM33</name>
        <name evidence="7" type="ORF">N0V83_009181</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PER33/POM33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3G8"/>
    <dbReference type="OrthoDB" id="5581259at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051645">
        <property type="entry name" value="PER33/POM33_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005344">
        <property type="entry name" value="TMEM33/Pom33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03661">
        <property type="entry name" value="TMEM33_Pom33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4364585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="280" mass="30700" checksum="B9C832EAC1924F75" modified="2023-11-08" version="1">MAPPPPASLPLGQRLAALAQTLQFAWFTGHVALLLTTLRYSLSYITFNYNSKWAAFSYRVAFLSACVTYGIVVYKAYRARTRAGKQGGMLTLAADENVQYLIMALVWLFSRQIPLAVLPFAVYSIFHVATYVRSNLLPTIQPPPAATTAGSKPQGSAASEAIGRFVKEYYDGSMTLVALLEIALWFRVVGSALLFQKGSWILLVVYTVFFRARFAQSSFVQEAIGQVTSRIDAQLANQSTPPAARQAWGTFKNIIRQAADATDIRRYVGGQPGAAPKKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3J9</accession>
    <name>A0A9W8Y3J9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3J9"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="426" mass="48193" checksum="80BEE336E19D5B8A" modified="2023-11-08" version="1">MDLGPPPGPEGSVAPHTTAPPKRQRADSGSSTSVESSRSVRTLTSDDLEYTHENGRTYGNETYYLPCVYMPNNDFKEQDRLSLQHQIFVHALQGKLTTTRLLPSTRRILDLGTGPGHWAVAMARQYPHAEVVGIDMTEWDLDTTEATLGDCRVTWELDDLDVWGREADIDDLTARLAQYDFFTETTLRNPIESPTRAEHSLPHSQPDTSVESLTIDLSTLEPQPEPGWHFSDAYELIHLRNMKGTFVHWEEVYAEIYKSLSPGGWVEIADYDLGQVPLPAGDHDPSALPMPTLRKLYKSLMEASFKSGRPLGTFYMHPSYLEEAGFTDVQTLHVNVPIGQWAEDEAQRTIGKLMLVVVMETFEASLLRLLTKWGDSEKIWTAEELMAEIEVAQQEVGEWCVRAERREVEGWCASFKWITGRKSWHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3V4</accession>
    <name>A0A9W8Y3V4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">aldehyde dehydrogenase (NAD(+))</fullName>
            <ecNumber evidence="3">1.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_008048</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>an aldehyde + NAD(+) + H2O = a carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.3" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3V4"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07106">
        <property type="entry name" value="ALDH_AldA-AAD23400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000009">
        <property type="entry name" value="Aldehyde dehydrogenase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000007">
        <property type="entry name" value="NAD/NADP-dependent betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044086">
        <property type="entry name" value="LUC3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="7">
        <location>
            <begin position="27"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4366412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="480" mass="52292" checksum="D8CD714E04A82938" modified="2023-11-08" version="1">MSPGKVSTLDFEKFYNIIDGKQRSSDKIHHGINPATGQELWDVPIASEKDLNDAVASAKKAFPAWRDTPIEKRKEAIGKFSEAYQQHTDEFTTLLCKESGKPRKFAAIEVGGVAGFVGFHLTLDIPAEKFEDDEKTVYTEYAPLGVCGAICPWNFPLILSAGKIAPALMTGNCIIVKPSPFTPYTALKLVELAQDFFPPGVVQVVGGNNELGQSLCLHPDIAKISFTGSIATGKKVMETSAKSLKRVTLELGGNDASIILPDVDVKKIAPEVVMGAFQNSGQVCVATKRIYIHESIYKEFLEEMVNFTKTVKVGNPDDGDNMLGPIQNQMQYERVKGFFADSKAKGYKFAAGEPDISSSSKGYFIQPTIIDNPPNDSRIIQEEPFGPIVPTQPWSDLEEVIARANDTNTGLGACVWGKDVEKASKVARRLEAGSVFVNSWEKPTPQAVFGGHKESGIGGEWGKTGLLAYCNPHVIHVYKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3Z6</accession>
    <name>A0A9W8Y3Z6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">S-adenosylmethionine transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PET8</name>
        <name evidence="12" type="ORF">N0V83_006860</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3Z6"/>
    <dbReference type="OrthoDB" id="276989at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000018">
        <property type="entry name" value="S-adenosylmethionine mitochondrial carrier protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45667">
        <property type="entry name" value="S-ADENOSYLMETHIONINE MITOCHONDRIAL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="16"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="115"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="214"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4367279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="304" mass="32671" checksum="6C3290B89C07FA5D" modified="2023-11-08" version="1">MQHALEPKRGQAWVESPYLRSLIAGGLAGTTVDLSLYPLDTLKTRLQSSAGFTASGGFSGIYRGVGSAIVGSAPGAALFFITYDSIKRSFAAPQTTISYNAEGKPYKEEVRDGGNEALGHMAAASLGEVAACAVRVPTEVVKQRAQAGQHPSSLETLRHILNQRHTRGTVHVWRELYRGWSITIIREVPFTVIQFPLWEALKKYRISQTGRDQVSGLEGGLLGSVAGAVAAGITTPLDVLKTRMMLAKEKEPMFSMLSSILKESGLRAFFAGIGPRVGWISVGGAIFLGSYQWASNALGDMKQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y445</accession>
    <name>A0A9W8Y445_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RAD52 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RAD52</name>
        <name evidence="7" type="ORF">N0V83_008760</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RAD52 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y445"/>
    <dbReference type="OrthoDB" id="206565at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000730">
        <property type="term" value="P:DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045002">
        <property type="term" value="P:double-strand break repair via single-strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.80:FF:000001">
        <property type="entry name" value="DNA repair protein RAD52 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.80">
        <property type="entry name" value="DNA repair protein Rad52/59/22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004585">
        <property type="entry name" value="DNA_recomb/repair_Rad52"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041247">
        <property type="entry name" value="Rad52_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007232">
        <property type="entry name" value="Rad52_Rad59_Rad22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042525">
        <property type="entry name" value="Rad52_Rad59_Rad22_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00607">
        <property type="entry name" value="rad52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12132">
        <property type="entry name" value="DNA REPAIR AND RECOMBINATION PROTEIN RAD52, RAD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12132:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD52 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04098">
        <property type="entry name" value="Rad52_Rad22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="270"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="310"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="327"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="364"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="408"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00077224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4365142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="608" mass="64840" checksum="42639DD4CE3CEBC7" modified="2023-11-08" version="1">MPAPGDQYHGGNVKNPYEERVVNGFTASEIATLQSRLNKQLGPEYISQRPGNGGGRVAYLEGNKAIALANEVFGFNGWSSSLGQVQIDYVDELQNGKVSLGLSIVVRITLKDGTYHEDIGYGSIENGKGKAASFEKAKKEAATDGLKRSLRTFGNVLGNCLYDKEYLKKVQAMKVKPIKFEEDNLYRHANFALPAQGEQGLVKREPQMTPVRPNQVLRTRTEHLSESQSADFDDEFDGNLFDEVDMTEHHRDELSFETLSAPDTVASKAIEATRPNGVPSARTSPIRNTGPPRQPNATMQPAQAGRGQPGLQQHQHNQGPSRVPPNVANQRHPQTPVQQQNQHRADLNRGRMPPPPGENGAASRPNGQLQQNPQQPAQNQPLRPTPPQGQPPANQQRPGPQGPGIANTPVNAQPSNNRPPVGFVTSRAAELLQASDTPSLNHIPAFNPHAESPIPKEQRTPGVDHARSVPIKREAVGAPPAPQPPPQGASRSSLPAGAAASFGRPTNFVNPHQDTNRRIGMPGAPNYAMSPSANRGAYKPPTFANGAAAASNGLKRERPALQDVSNTGGTGGGNGGAVNSSDVGGPDAKKQKTEAPTGVENIAAAVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y459</accession>
    <name>A0A9W8Y459_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Origin recognition complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ORC2</name>
        <name evidence="9" type="ORF">N0V83_007365</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the origin recognition complex (ORC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the ORC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y459"/>
    <dbReference type="OrthoDB" id="346673at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000637">
        <property type="entry name" value="HMGI/Y_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007220">
        <property type="entry name" value="ORC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056772">
        <property type="entry name" value="RecA-like_ORC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056773">
        <property type="entry name" value="WHD_ORC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14052">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14052:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04084">
        <property type="entry name" value="RecA-like_ORC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24882">
        <property type="entry name" value="WHD_ORC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00354">
        <property type="entry name" value="HMGI_Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Origin recognition complex subunit 2 RecA-like" evidence="7">
        <location>
            <begin position="251"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 2 winged-helix" evidence="8">
        <location>
            <begin position="516"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="145"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="496"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="500"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU368084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4366838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="588" mass="66134" checksum="93C9473CB7885FDC" modified="2023-11-08" version="1">MVGTKRRRDDDDTDDGTPRKLRARIADEAPDEQDLFEAQTPSRRGRTLQLAEDGTPRSILKKSRLADAVHDTPRSSRKLVFETPTKSGKDETPNGTPAIVRNADRSARRKSNRRMLERTLNGADSDDEALEEDEILAEQILGEEEDEVADHAQEEAAQEDNVPVPETPSKRGRGRPKGSGKGGRPVGRPRKERQRSPTPPTELPPHEIYFWQNRPAGTKTSNNTLSAQTLLNHDEFFQTIQTYQDPHEPERDFLLELHGRAYDQWIFELDEGFNICLYGYGSKRTVTERFTACLYHHLLERFPYKGTKKIPKIVVVNGYTAGTVVKDILTTIASTVVPANLKMPNQLATLLDFVLEHLSNHPPSHPIHVVINSIDSPYLRKTPTPSMLARLASHPNINLICTADTPNFPLLWDVGLKTQYKFLFHDTTTFAPYDAEIDTVETVNELLGRSGRRVGGRDGVGFVLRSLPENARELFRILVMEQLALSLVEGALGEDEDVPATSSSKKVPQTKNTPSESSQGVEYRVLYHKAVEAFVCSSEVGFRTLLKEFHDHQMIESRKDAMGTERLWVPFRQDELEGLAEDLAAEAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4V4</accession>
    <name>A0A9W8Y4V4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_007094</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4V4"/>
    <dbReference type="OrthoDB" id="5313288at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4367511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="267" mass="30141" checksum="5F50FA4AE727093B" modified="2023-11-08" version="1">MAPQLDSLPPEILFNILSFTEPTCNPALVSYPLNALAETSKHLNTIVEEYARSLLKQHANITPPKNSRVFTCRRKWLGEICQFCKKPSKRRAILYGTLTCCRTCDKAQFPKMTMTKATQDHALSKLDLFTPNALHPHLAPLSIGNYTVMGGTATMILAADVVARRDHIYAVLGNTRALDAAYMRKRVAAHQRMIQHMGIVWDCEDAAWREAYEPRVKEMVTVKQKGPKSMRSEDSRREYVKMALTKEWDDMGVREGGGTKDTAIEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4Y8</accession>
    <name>A0A9W8Y4Y8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable kinetochore protein NUF2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">NUF2</name>
        <name evidence="18" type="ORF">N0V83_006489</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NUF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4Y8"/>
    <dbReference type="OrthoDB" id="8194677at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051383">
        <property type="term" value="P:kinetochore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045132">
        <property type="term" value="P:meiotic chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.60">
        <property type="entry name" value="Ncd80 complex, Nuf2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005549">
        <property type="entry name" value="Kinetochore_Nuf2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041112">
        <property type="entry name" value="Nuf2_DHR10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038275">
        <property type="entry name" value="Nuf2_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN NUF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650">
        <property type="entry name" value="MEMBRALIN/KINETOCHORE PROTEIN NUF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03800">
        <property type="entry name" value="Nuf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18595">
        <property type="entry name" value="Nuf2_DHR10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="13" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Nuf2 N-terminal" evidence="16">
        <location>
            <begin position="25"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Nuf2 DHR10-like" evidence="17">
        <location>
            <begin position="280"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="362"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="159"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="320"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="371"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="389"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF18595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4368134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="461" mass="53686" checksum="95E27D51F0F43688" modified="2023-11-08" version="1">MSYNPRMSTVRTTSHNQRAPAANEHDAFMTLPDSEIAGCITDIGINFSVADLQKPNPQIIQKVFEWLAELLMNTTREVLAPAMRAAAEDLCGGDAERIFTSDTRDLMGFFVVLKKLLRECGIHDFTFNDLYKPTHGRLVKIFSYMINFIRFRESQTEVIDEHFNKAERTKLRIEQLYNDKQAKEMQLADLERNRAATQKLMQEKEKRNNELKNRLLELKRGQEAVADKLERARAEQTRLKELLQQRVENKENVQREVLKLKPYTQQSPTALEESLRDLNDRLTVDKTQIDTLDRRARALQTSTDSFGVVATDVTSCTRLLTDVQADLSKEEEELSKAARHRDALADRSNNVRDVERQERLLQKQLGNVNARTDKLKTKADEEAERARKRMEELRETHSKLAEERGEKGREMERRRVRIEQTEKKMAELKDNIENEVHAAHDEYLKMESHIKLYITEMEQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y587</accession>
    <name>A0A9W8Y587_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein argonaute</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ago1</name>
        <name evidence="3" type="ORF">N0V83_008472</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y587"/>
    <dbReference type="OrthoDB" id="10252740at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02846">
        <property type="entry name" value="PAZ_argonaute_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04657">
        <property type="entry name" value="Piwi_ago-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032472">
        <property type="entry name" value="ArgoL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032473">
        <property type="entry name" value="Argonaute_Mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032474">
        <property type="entry name" value="Argonaute_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045246">
        <property type="entry name" value="Piwi_ago-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16488">
        <property type="entry name" value="ArgoL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16487">
        <property type="entry name" value="ArgoMid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16486">
        <property type="entry name" value="ArgoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01163">
        <property type="entry name" value="DUF1785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Piwi" evidence="2">
        <location>
            <begin position="622"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="929"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="979" mass="109689" checksum="F84FD0A529FFAD7D" modified="2023-11-08" version="1">MGQPAKKHAQREKQSKDSSSGSSSGSRDPTQRSAPKSIPRVDGNRDPNTHAVDYAKPNDLKNISEFLGLAGWYTARGIDLPDSLVHRPKNFNQYGKECGIMLNTFHVVKTPNIIVHQYDLNYTGDAKPEEYTKRVLLKKIWDSRAVKSALGESANLWIWDGNKLAWSGKKLDRDDTRITCDLDAEEGRAPNPNRRGNKHTMHLRWTRKIDFNHLQAFLNGQASWGNECIDTILFLDHLLREHPSRQYTQIKKNFFQRGEQRFDLGGGIEAFKGVFASLRPVLDDKFQKSLSVNVDVANGTFWRAQELTRAIGQVFNCSPPQFAAMFKNAQRAWKDSVLKKDLRRFKRVGVSTTHTKEPSQWTIDEFVNLDATQAMFPDPDDRRPGVDPRQLRQISVFDYFKQKYGITCLLGVPVVKMTKQIRKGPVYMPIDVLKIDQNQRYNIKLSDTQTSAMIKFAVTLPKDRWAAVQQGVRLLNWANDPYLQHYGLQINANAAKVKARILPSPNVHFGTGSKEPTIKPQDLIAGRWRLDGRKFAMNNKDRPIKAWGVCCIQGRGSPPPQAVEAFVQKFVQIYEGHGGLVSAHPQHGKKPWMGPGNLADGGELIQKVWNQTGNRYATQPNLLIFIVTDRNIDVYRRIKKSCDIRFGVASQVLQAKHVMSASPQYISNVCMKVNAKLGGATCVAKSQLIPKIAPKAASTPTMVVGADVSHPAPGAGSGEAASFAAITVSADPLFAKYWAEVQTNGNRVEMVTSANIDEHFGFMAKNWMQKIGQGRAPQRVLYIRDGVSEGMYAAVLAEEVRDMKECFKKIGCKDMPKFTVVIAGKRHHIRFFPDAGKGDRNNNPLPGTLVESGCTHPFEFDFYLCSHVAIKGTARPIHYQCILNEGEWGAAELQQFIFEHSYQYMRSTTPVSLHPAVYYAHLAADRARAHQNDNPVSSGKKESKADQESSTGSSSKNVEIAPLMAIQNARGLKDVMWYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y597</accession>
    <name>A0A9W8Y597_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminoglycoside phosphotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008488</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y597"/>
    <dbReference type="OrthoDB" id="5210591at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051678">
        <property type="entry name" value="AGP_Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310:SF59">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="2">
        <location>
            <begin position="93"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="347" mass="38328" checksum="8E12A0039FE3D641" modified="2023-11-08" version="1">MSRGTYAARPARPSSTVSSSSSSSASSRTHSATPDFPSIQKLIRSVYRSSKITVQQAEQLQARLHQVFLTRMVDGSILVLKCPPACNTRLLRHEKSLLENESKALETLHNYLPRVPVPQLLKYDGQGTSLGSPYIMMSYIPGRRLSELAPYLTAAERSTIDQTLGAYVRALTTPPATQFGMTHRVFARKGSDSWHEAFSALFEAVLRDAEDMLVTIPYESIRYYVGKQAHFLNEVTEPRFVALHACDPTNVLIDETTKRVTGLVGFSDVIWGDPLMTGGLEGGSAAFFEGYGSRPVQTGGVKARMLMYAAYKATLRIVTHHYRPHLGTDENEARRELSIALNALAAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6E7</accession>
    <name>A0A9W8Y6E7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_007164</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6E7"/>
    <dbReference type="OrthoDB" id="1630758at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF94">
        <property type="entry name" value="TNF RECEPTOR-ASSOCIATED FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02176">
        <property type="entry name" value="zf-TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="59"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="TRAF-type" evidence="7">
        <location>
            <begin position="199"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="4">
        <location>
            <begin position="199"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="313"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="432"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="432"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="474"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4367580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="485" mass="53586" checksum="6C4013A19F28DCED" modified="2023-11-08" version="1">MPWVDHGMLVGGPPGSPSPLALRASNSPSSKRRHSRIVKVPPEFQSLEYVSTPDSNLVCLICHTPFDKPVQLSCEHYFCRECLDHAWAPQASGRKTCPTCRSKVETEKDIRPVPKIIETMLDELVVKCPNTKAGCSWADHRVNVNDHVMLYCEYTPVECSGTDCRLHISQKDFHKGCLHYTVSCEDCHTSLMKRDLEEHKRNSCANRKTSCTLCSAELLRLDLKSHINKDCPKHVISCQGTIVGCKFTAERAEVVLHEVNCAMATMAPHFREQQARIERNEARMEPLARKVGILEDGLCTITNMLYPANANDQSFPVTDPLDPNDTDPQAPTPDFRLPPASFPPVPPGNDGAPTQPPFDSQVHHLLTLHDSLREEVSRIANALTDLDGRTNMMIINESQRAKDEMLHANAAISSLRMHLQWLVSATIHQRTTSAGSSTARTAGSTSVNAGSSSGTRSASTRPGPSATLQGPFRRLSDSTRQDTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6H9</accession>
    <name>A0A9W8Y6H9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7905 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6H9"/>
    <dbReference type="OrthoDB" id="4739136at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057227">
        <property type="entry name" value="DUF7905"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25482">
        <property type="entry name" value="DUF7905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7905" evidence="1">
        <location>
            <begin position="81"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="192" mass="21809" checksum="B8D604416A723E26" modified="2023-11-08" version="1">MNRFYKSRAWYFGLESMIPVSRKLVSPMLVGFADRVQMRPKYDVNSTKSFALWDQSPGVNKHMQYARLDKIYSFGIQKTSYKVELTAMWYPNQIMPVWGLAIRHTEWAVHLAELERLSVGSQASWGDVVSTFLPDDGVRSSVTESDLGLERLSLNNELEMPPRDGIRILMEKLMQLSEIVSSVTAAEGGVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6M7</accession>
    <name>A0A9W8Y6M7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL23m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">MRP20</name>
        <name evidence="5" type="ORF">N0V83_006923</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6M7"/>
    <dbReference type="OrthoDB" id="275582at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12059:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL23M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12059">
        <property type="entry name" value="RIBOSOMAL PROTEIN L23-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4367342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="221" mass="26014" checksum="1AAF5CE9D2C5A5F1" modified="2023-11-08" version="1">MDAIPITKKLIAFGSKKIYLPKFTVALIRTPGISPYHARFLVPLDFSKYDLRDYLYHAYNVKCFNIRSFVKQMPVRDTSQQPRHWFRPESKKYMTVEMEQPFVWPEVPDLEPWGQKEKKKQDREGITSQGVVYQEEAREAARTLRDQARMLLHRREPLVDQSIEKKKVKGVELSPAEQAKEEQRLARERELESMSRLKLWEEKRTDKIVQGDGASGYTIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6R3</accession>
    <name>A0A9W8Y6R3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_006300</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MSOX/MTOX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6R3"/>
    <dbReference type="OrthoDB" id="2219495at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051698">
        <property type="term" value="F:saccharopine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008115">
        <property type="term" value="F:sarcosine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045170">
        <property type="entry name" value="MTOX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961:SF26">
        <property type="entry name" value="L-SACCHAROPINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961">
        <property type="entry name" value="PEROXISOMAL SARCOSINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="6">
        <location>
            <begin position="9"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4369215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="440" mass="48924" checksum="C706AEC43CEB8072" modified="2023-11-08" version="1">MVISKSDPIVIIGGGAFGLSTALELSNKGYTNVTIFEKDEEIPSRWSAANDLNKIMRAEYEDEFYTNLAVEATHAWQTPVYAPYFHRVGFLNCVSGAAPQKAADTMRRFQAAAAKHPEMAPFLHDISSVEDIRNIAGAWQFTGDLSGWKGYVCKYDGYVHSANALRGIYREARKNGVRFFLGEKAGAIEKIEYHGTGRNRMSVGVKTKAGRFHAAKLVIVTVGAAAHKIVPELGTEVSAKSWSVAHVRLTDDETAALRGIPVTYARDLGFFFEPDPKTNLLKICPMGGGYINTNPSTGVSEAPEGVMRFVPKEDERKMRELLRQTLPYLADRPLVDKFICWFADTADSDFIVDYVPDTSASVMILSGDSGHGFKMFPIVGQWVDNLLNEADGKQSISRWRWRVPKPKAKGESFDDDVSWRIGDVREIRDVQDEEPVKSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7J3</accession>
    <name>A0A9W8Y7J3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein transport protein SEC13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Protein transport protein sec13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SEC13</name>
        <name evidence="16" type="ORF">N0V83_007138</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules. It also functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. SEC13 is required for efficient mRNA export from the nucleus to the cytoplasm and for correct nuclear pore biogenesis and distribution.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The COPII coat is composed of at least 5 proteins: the SEC23/24 complex, the SEC13/31 complex, and the protein SAR1. Component of the nuclear pore complex (NPC). NPC constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat SEC13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7J3"/>
    <dbReference type="OrthoDB" id="364224at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090114">
        <property type="term" value="P:COPII-coated vesicle budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032008">
        <property type="term" value="P:positive regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032527">
        <property type="term" value="P:protein exit from endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000017">
        <property type="entry name" value="SEC13 homolog (S. cerevisiae)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037363">
        <property type="entry name" value="Sec13/Seh1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN SEC13 / SEH1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024:SF2">
        <property type="entry name" value="PROTEIN SEC13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="12" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0811">Translocation</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="7 15" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="56"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="208"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4367554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="301" mass="33115" checksum="83DE6DBBD841FE37" modified="2023-11-08" version="1">MAERGQNIDLLSIEANETQHDAVLDYYGRRLATCSSDKTIKIFEVEGEKHTLVETLRGHEGAVWCVAWAHPKYGNILASSSYDGKVLIWREQSSTWQKIYDVALHTASVNLVAWAPHEAGCLLACASSDGNVSVLEFKDNAWTHQIFQACGSGVNSVSWAPAVAPGQVISASGNQAGAARRFVTGGSDCQVKIWEFNAETGWQNTEILPGHTDWVRDVAWSPTILSKSYIASASQDKTVRIWTSSDLRDWKSTVLNVEAVAWRVSWSLSGNVLAVSTGDNRVSLWKERLSGGWECVKTIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7X6</accession>
    <name>A0A9W8Y7X6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">AA9 family lytic polysaccharide monooxygenase</fullName>
            <ecNumber evidence="15">1.14.99.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Endo-beta-1,4-glucanase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Glycosyl hydrolase 61 family protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">N0V83_004916</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Lytic polysaccharide monooxygenase (LMPO) that depolymerizes crystalline and amorphous polysaccharides via the oxidation of scissile alpha- or beta-(1-4)-glycosidic bonds, yielding C1 and/or C4 oxidation products. Catalysis by LPMOs requires the reduction of the active-site copper from Cu(II) to Cu(I) by a reducing agent and H(2)O(2) or O(2) as a cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>[(1-&gt;4)-beta-D-glucosyl]n+m + reduced acceptor + O2 = 4-dehydro-beta-D-glucosyl-[(1-&gt;4)-beta-D-glucosyl]n-1 + [(1-&gt;4)-beta-D-glucosyl]m + acceptor + H2O.</text>
            <dbReference type="EC" id="1.14.99.56"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">Has a modular structure: an endo-beta-1,4-glucanase catalytic module at the N-terminus, a linker rich in serines and threonines, and a C-terminal carbohydrate-binding module (CBM).</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the polysaccharide monooxygenase AA9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.56" evidence="15"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7X6"/>
    <dbReference type="OrthoDB" id="3238762at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030248">
        <property type="term" value="F:cellulose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21175">
        <property type="entry name" value="LPMO_AA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049892">
        <property type="entry name" value="AA9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005103">
        <property type="entry name" value="AA9_LPMO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33353:SF9">
        <property type="entry name" value="ENDOGLUCANASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33353">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12560)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03443">
        <property type="entry name" value="AA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6 15" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="8" id="KW-0186">Copper</keyword>
    <keyword evidence="10 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12 15" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Auxiliary Activity family 9 catalytic" evidence="16">
        <location>
            <begin position="17"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4370398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="249" mass="24964" checksum="879C1D467BE30EB5" modified="2023-11-08" version="1">MHQQNGDRSCANEAIGGAHYGPVLVYMSKVADAATADGSSSFFKVFEDTWAKNPSGSSGSDDYWGTKDLNTNCGKMDVKIPSDLAPGDYLLRAEAIALHSASGLNGAQFYITCYQITVTGSGSSSPAGVSFPGAYKATDPGIQINIYQNLATYVAPGPSVIAGGTEAVAGKAGSAVTATGGSSQPTATVSKEAASSTLKTSVKATSAAASAQPVPTTGGGSTGGCTVANIHHGDLGFGKDGIVLSVRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8H1</accession>
    <name>A0A9W8Y8H1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF202 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4370011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8H1"/>
    <dbReference type="OrthoDB" id="2555434at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38646:SF1">
        <property type="entry name" value="DUF202 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38646">
        <property type="entry name" value="YALI0F00814P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="15518" checksum="9E860EBE82DACFB6" modified="2023-11-08" version="1">MFEGRHVADRYRLRMHRARSVVLTSSELVEIRAAQRTFEGAYIRTAIGQFSFALIVLKIFTAEFYSIGALFAVYGAGILFISLFRRQQGNKQFFAEMGEDGLHRKKFRTSGNVVSVLTALSISAYICLLVLTLRLET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8P0</accession>
    <name>A0A9W8Y8P0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_005333</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8P0"/>
    <dbReference type="OrthoDB" id="3257095at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF2">
        <property type="entry name" value="ACID PERMEASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="333"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="376"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="413"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="459"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="490"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4369571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="526" mass="56941" checksum="AB041E7177637714" modified="2023-11-08" version="1">MSGLLAKQAEHSSPQEVKSRYDDVSDTSEKRGTTADVASMWRMGKTQELLRNFRFVSIFGFSMILMASWETMLGTAGIGLIDGGTAGMIWMYLIVWIGFICVNTSMAEMGSMAPTSGGQYHWVSEFAPPEYQKFLSYVIGWLCVLGWQTGAANTAFLAGTQIQGLIALNIPDYSFEAWHGTLLVFAVSAFNVVFNTFFVKKLPIIEAVVLFVHILGFFAVLAVLWATGPIVDARATFTTFNDYGVWNDNGLATLSGIVAVVLPLLGADAAVHMSEEVRDAAQIIPQSMITTTLANGAMGFVMLITFCSVLGSIDDALASPTKQAYLYVFYNSTGSVAGASVMGALVILMAIFCNLSITATSSRQLFAFARDKGVPFYATFAYVPPAWNVPINAICLSFAVSCLLSLINLGSSVALMNIASLSTCAIIASYIVSISCIALKRIRGEQLLPSKFDLGRAGLPLNIVSIVFLIFVFLFSFFPMGPKPTPAGMNWSILMFGGTMIFSLVYYLVKGRNVYVGPVEYVRKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8S9</accession>
    <name>A0A9W8Y8S9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleoside-diphosphate-sugar epimerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_005253</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8S9"/>
    <dbReference type="OrthoDB" id="9975943at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14097:SF8">
        <property type="entry name" value="NAD(P)-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14097">
        <property type="entry name" value="OXIDOREDUCTASE HTATIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4370732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="26604" checksum="5AF0EE83D47B3B1C" modified="2023-11-08" version="1">MHLILTGATGLVGASVLDNMLAQESISRISILSRRPVKMAEGHEKAKVILHKDYKTYDKALLEELKDANGCVWAQGVSQNAVGKDEYIEITHDYPLAAAKAFSTLHPDSPFAFVYVSGEGATQTPGMFTPIFGRVKGQIETTLFEFGKQTSNFKVYNVRPGGVDWTSHPEIHPFMPKQDLYKKVLVPPLNLVWKGLMTPTQPMGRIFTELAMSKGEPLQGSGIEMEGRLVRNSAIRRMAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8Z2</accession>
    <name>A0A9W8Y8Z2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 17">Mevalonate kinase</fullName>
            <shortName evidence="17">MK</shortName>
            <ecNumber evidence="3 17">2.7.1.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ERG12</name>
        <name evidence="21" type="ORF">N0V83_005008</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Mevalonate kinase; part of the second module of ergosterol biosynthesis pathway that includes the middle steps of the pathway. The second module is carried out in the vacuole and involves the formation of farnesyl diphosphate, which is also an important intermediate in the biosynthesis of ubiquinone, dolichol, heme and prenylated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(R)-mevalonate + ATP = (R)-5-phosphomevalonate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17065"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.36"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:17066"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="16 17">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.36" evidence="3 17"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8Z2"/>
    <dbReference type="OrthoDB" id="1652964at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000027">
        <property type="entry name" value="Mevalonate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00549">
        <property type="entry name" value="mevalon_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290:SF2">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 17" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 17 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 17" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 17" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="14 17" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="11 17" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="13 17" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="6 17 21" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="19">
        <location>
            <begin position="224"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="20">
        <location>
            <begin position="393"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="448"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="469"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ4370487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="588" mass="64060" checksum="9B80C0E30708E03A" modified="2023-11-08" version="1">MAANGTMRRKTHDAPGETLEAIAVPREIMANLCPRAAVTLQPRTMSSHATQTPEHSPVDAMPVQLPPNVASTAAPPTPGMLHRKQSSPMMPPFMVSAPGKVIVYGEHAVVHGKAAIAAAISLRSYLHVSFLSKSNRTVKLRFPDIQMEHTWNIDELPWDVFSKTGKKKYYYDLVTSLDPDLMAAIQPFIDQVSPKAPESIRKIHHASACSFLYLFLSLASRKVPPCVYTLRSTIPIGAGLGSSASISVCISTALLLQIRALSGPHQDQPPQECELNIERINRWAFVGEMCIHGNPSGVDNTVSSGGKAVLFQRRDYDKPPLVVPLYNFPELPLLLVNTRQSRSTATEVAKVANLRSVHPAITENILNAIGLVTESAHKLLTSPDFDPTSHASLKHLGELVTINHGLLVSLGVSHPKLERIRELIDHTGIGWTKLTGAGGGGCAITILKPQPPALSNGHGNGTHHHDSSSDESDTSSENDPDCSASIISNGTKLKYKILDSLEVKLEHEGFEKFETTLAGDGVGVLWPAVLHNGNEEEGGEEIDQEKFLKAEGTIGIERLVGVVSSRRKVVREVREGWKFWRPWETPEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y930</accession>
    <name>A0A9W8Y930_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histone deacetylase hda1</fullName>
            <ecNumber evidence="3">3.5.1.98</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">HDA1_1</name>
        <name evidence="3" type="ORF">N0V83_005545</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y930"/>
    <dbReference type="OrthoDB" id="424012at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019154">
        <property type="entry name" value="Arb2-like_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09757">
        <property type="entry name" value="Arb2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arb2-like" evidence="2">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="174"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="193" mass="21549" checksum="E7AE0CB273E5DE69" modified="2023-11-08" version="1">MGIGDAYLGLVDLISHNENTTDSEEPVVECLIGFVAGTTIQSVRRATDDNIASWYHAHSKIFVKNSHFAWDPSRQRKLRKKLGNLVKSPKDTLDDMLEEHLEEVQNLLLEKKSEFEERNGHSSQTDEGQQFSVGGLRSPPLPGGIHTPKFPDSGVRHNDNLANLKSPKMPPLGMFSVSSMSPRSPRSPMKRTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9A5</accession>
    <name>A0A9W8Y9A5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carrier domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_005240</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9A5"/>
    <dbReference type="OrthoDB" id="429813at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051414">
        <property type="entry name" value="Adenylate-forming_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43439:SF2">
        <property type="entry name" value="ENZYME, PUTATIVE (JCVI)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43439">
        <property type="entry name" value="PHENYLACETATE-COENZYME A LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23562">
        <property type="entry name" value="AMP-binding_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="3">
        <location>
            <begin position="259"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4370719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="761" mass="84989" checksum="A76640F25FC58E7B" modified="2023-11-08" version="1">MEGDGHGIEELRQMDMVGVGGAALPAEIGDRLVKNGVHLISRFGSAECGFLLSSNRDFDVDKDWQHLRNYNPPKLLDFEQREDRLAELVIRPGWPHMAKQNRSDGSFATADLFAPHTTIKNAWLYHSRADSQLTLITGKKFDPAPLEDAIAISSHLDDVLIFGNNRPYPGALLLRSEQSNNVSDLELLNAIWPVVEKLNGESQDHARIRLEMLVPLVHQSQPLEKSSKGTMIRRAAESRFEDDIERAYGAQEHDQVVDVNDEDLPQHLTGLIQTITSQSAELSEDTDLFSYGVDSIACMQLRSSLRGLVPNSTDQLPMSIVEDCGTIRRLTDYVIRKRHGESNTDGEDEEQLMLDLVKEFGSWEQSASEDTLNRSRDKNAAGELVVLTGATGGLGAYILDLLQKIANVDTIYCLVRGADEDVAKERVNKALKQRDLESLVEGPSAKVKVIPAQLSDERLGLSDELYTHLAKKATSIIHVAWTVNFRLKLRSFINDNITGVRNLINLALNEHRSTPLRFTYCSSTAAIMHGPLDESGCLPERLLSDPSAASPLGYSRSKWVAEQICAEASKRTSLRGRIAVVRHGQLAGDSVSGVWNTKEAWPMMLSTARLIKCLPDLGDEPVDWLPVDTAAQAFLEATGVNTGSESAKAEDISIYHVLNPHQRPTWHEMLQWLHKKKEEFEIVSPQEWVKKLEEYDSADHSAMKLLGLWKDSYGNGPGEAKQRPQFSMVETRKHIPALRDVKPLDEAYLGSIWKWVQENVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA04</accession>
    <name>A0A9W8YA04_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cell surface protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005577</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA04"/>
    <dbReference type="OrthoDB" id="5310497at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021476">
        <property type="entry name" value="Egh16-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34618:SF1">
        <property type="entry name" value="SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34618">
        <property type="entry name" value="SURFACE PROTEIN MAS1, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11327">
        <property type="entry name" value="Egh16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040871984" description="Cell surface protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="30417" checksum="376C477E8B59A55F" modified="2023-11-08" version="1">MHFSQSFALTAAFVACISAHGVVTEIKGANGVSMPGLTIQDGTPRDCSSNGCGSQADTAIIRDREIASGKATPLGRTQGNGPVDASVMVGAFMGAGTAPPSNQGASASVGVEDDIPQNAQKRQLLGGLFGGGGNGKAGATGITGLLGGGGDKVSGPPEARVAAAQGAGQSSGLPTCADDGTVTMTLRQINQDGAGPFTADVDGTSGGTDEAAMQPATVTKDVPGLGVQGLSLATNTEFEMQVQMPAGMTCDATVAGTSNVCVMRVRNGAAAGPFGGSVAFTQSTAARKRAIAYKLRKRMEMNRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA08</accession>
    <name>A0A9W8YA08_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EthD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003745</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tpcK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4371972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA08"/>
    <dbReference type="OrthoDB" id="2519291at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009799">
        <property type="entry name" value="EthD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07110">
        <property type="entry name" value="EthD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EthD" evidence="2">
        <location>
            <begin position="48"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="168" mass="19076" checksum="4AFB57D741FAC511" modified="2023-11-08" version="1">MSDLTNPADISSVDTSSNSKINCYGPKARRKVFNNRMGVTVFMFVPRKPGMSIEAFKDHYENKHVPLVLRALGDAKPVSHTRYYCQRNPAAQGDAEVPPPLLYMGDVNTIDYDCITIMELEDEAHFKRFNEAFETSPLKKDVEADQKIFADESKFKILAVESPRVSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA66</accession>
    <name>A0A9W8YA66_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide-binding protein alpha-3 subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_004666</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA66"/>
    <dbReference type="OrthoDB" id="5817230at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000692">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF369">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-2 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0807">Transducer</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="574"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="577"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="591"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4371449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="625" mass="71587" checksum="B861A3AF0558253E" modified="2023-11-08" version="1">MADTPLDEKTLFTLRSAVGANRERLRQGIDGLREIHDRWKGSNGTSINLIAQLTALKSSLENMQEWLKYEVHNLHPQLLSDLDVLMTSCGLLVRHLHALNARLKQPDHDAVDFAIKLKYAVGSRSMERLRKVAQRQNDAVSLLLAACKCHALAQRKILLYKSRQIRNEDASSLKTLARSTRWNGGCMRVLTQVSRMIQCLRFMFHLKLKRSTPERGPTPTGEEYDHAAAANRSEAIDRALAEEATALRRETKLVLVGQVNSGKELIMHQMKVLYAEGYYPMEERMTFRYAVRSTIWVLVHAIIDLLKDTGVPLTPKLNQDFAILLHEVETADMQRITPAAIKAVENIWTSPEFSALYIRNFEIDFPQYAPYFAQELPRIANADYVPSEADIMRLNQSMGGIKELRFSWDELDVHLFNINGFIPDQFRKRWFHQLEGASSLVYTVDVSLYDRPYLGHSTESQLLDDFATFESWVSSPKFANSSIILLLNNFTRFREKLQYSPLATFFPDYRPGESDPETSARQYILRRFKEVNRNQLSIYSFWVDLDMSENQHLYAALKKTLQRIQQRKARNEVWNESMSSMGSRSRSASGLTSMLSPSRSFSNKRAGTDSSRVISPVQSDGTEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAB2</accession>
    <name>A0A9W8YAB2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_003773</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAB2"/>
    <dbReference type="OrthoDB" id="2985014at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000013">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF9">
        <property type="entry name" value="MAJOR FACILITATOR-TYPE TRANSPORTER HXNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4372000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="421" mass="46391" checksum="F57CDEE697BD75E0" modified="2023-11-08" version="1">MAATTYEPENVNVNEKAPRMSESSIEKGQGVGTAMSIVDTAAERAYVMVGWGAMALLQCATTNFAGMLVLRLFLGAFEAGFFAGVVFYLTLFYTRGELGFRIALFFGSALLAGAFSGLISFGVFHIENGIPGWKWLFIIEGVMTVIVAFIAYAWLPASPQTAWFLNSAERAVAERRTLRDLSSAGSHDFSVKAMFKEWHTWKMIPWCIIAFTYPVAFSTTSNFLPQIVQRLGYSTVKTNLWTVAPNAVGFVVLLCICKSSDYFRERTFHIVGALCISLVGLIILATIDVLHNKAVAYFACFMLASGAYIPSCLVHSWHNNNNLNENARAATTGLLVGLGNLGGIVSAATFRAQYAPKYVPTLIGTAACNVTCICFTLFLGVWMKMENRKRDRQQGVKLRAEDVRTDQLADGEASEQWRYFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAG4</accession>
    <name>A0A9W8YAG4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Stress protein DDR48</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAG4"/>
    <dbReference type="OrthoDB" id="203279at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="245" mass="25724" checksum="EA4D3E7BCBA766F1" modified="2023-11-08" version="1">MSYNDNDRETYGEERGSGGSGGFGSSNRNDDNEYGSKTSGYGNDNEDSSSGNKQSRFGSNDNEDSSYGSSNKQSSGYGNTDNDDSYSSKKTSGYGNNDDEASYGSSKKTSGYGGDEDSSYGNKKSSYGSSNDTEDSYDSSNKQSSGFGSSNRNDADDNDSYGSRNQQSSYGGSNDTEDSSYGSGNKSSSGGYGGSSTTRSDDDESYGSKKQSGGFGGGNDNEDSSYGSSRNNDDSYGSSKRDNDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAY4</accession>
    <name>A0A9W8YAY4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Telomerase reverse transcriptase</fullName>
            <ecNumber evidence="2 13">2.7.7.49</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Telomerase catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">EST2</name>
        <name evidence="16" type="ORF">N0V83_005132</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.49"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Chromosome</location>
            <location evidence="13">Telomere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the reverse transcriptase family. Telomerase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.49" evidence="2 13"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAY4"/>
    <dbReference type="OrthoDB" id="289721at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000333">
        <property type="term" value="C:telomerase catalytic core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003720">
        <property type="term" value="F:telomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01648">
        <property type="entry name" value="TERT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021891">
        <property type="entry name" value="Telomerase_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003545">
        <property type="entry name" value="Telomerase_RT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12066">
        <property type="entry name" value="TELOMERASE REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12066:SF0">
        <property type="entry name" value="TELOMERASE REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12009">
        <property type="entry name" value="Telomerase_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01365">
        <property type="entry name" value="TELOMERASERT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00975">
        <property type="entry name" value="Telomerase_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13 16" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13 16" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="9 13" id="KW-0779">Telomere</keyword>
    <keyword evidence="5 13 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="15">
        <location>
            <begin position="543"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="334"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="367"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4370611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="899" mass="101514" checksum="B264D9DB862C428A" modified="2023-11-08" version="1">MKRKLASHGGGGSRKKARRAVHAARAVDQSASATPAATDHPVLRRLYPQVLTLRHYLLSRLSASSKSRRRRISQLGQADAAQDASSTHNVDNELGQLLDAALVGISSKTSTGCEEQVVKERDQDIDIFTQQRSQATNGGTFKPGYFQQSEIVDFVVWCLFRRSTAHKPSHLLCHGFQRSGHTHVPHPVDKDPDSSIPGLVARYPNSNVQSLKKPLWCRLNALLGQGGDCIMMDLLLHSSIFIPVDDNLGNYYQLSGMPLSELKLDRISTDTPANPETNLGASRKPVNSISTDRSPGTIVFVRSRMLYAKAALNAKGGVRFGMRHIHVLNRFPRREDSQQTGVPLSAKPEWGKNKLQPTGNAFAAPEPADHNRKDDTSNPNTRTVEQTCFTDMACPTAHVSAFCRAVTSKVIPHGFWGSDNNKRTIMYWIDQFISLRRFETITLHQVTQEFQIVTLAWLQLPGQDATTKLAQSDFSKRKEVFFEFVYWFFDSFLVPLIRTNFHVTESNVHRHRLFYFRHDVWHLLTEPALMTLRLSMFEEMPTERITKHLSARQLGFSKIRLLPKKYGFRTIMNLKRRQQVLRNGVMSLGRSINTVMTPVFNAVTYEKSQQADRFGCSLFSVGDMFPKLAAFKASLRERHLGNSPLYFAKVDVQSCFDTIPQSRLVSMIDSLMSLQAYQTGKHVEISPMGPLQRLDGQHVDPMPLTRYVAHSGAAAYMNSFSELVQNKLVGTKANTVFVNTNLHRVETKDDLMQLLREHVERNLVKIGKRYYRQKTGIPQGSVLSSILCNFFYAELERDVLSFVLDNDCLLLRLLDDFLLITINREHAERFMRVMHRGHEDYGVVVKTAKSLANFDVVTDDGHQVPRSASQVSFPYCGVFIDTQTLEVSKKTDPSAMAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBV8</accession>
    <name>A0A9W8YBV8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_003937</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBV8"/>
    <dbReference type="OrthoDB" id="9368434at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="428"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="125"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="601"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="601"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4372164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="737" mass="82597" checksum="5624895D4311B345" modified="2023-11-08" version="1">MSAPHGQNCHPRRRPIKGQVSPLSTHGMHLRKSCTFSSPKSLSTDLCDLENQHFMPRRSPTSTESLEELLQDSSVRRVANLLKDFDKKIAGHRAASTTGANILNDPEVLPVPSFVLDNTTLDSTPMDIDSKPSVVEQNPHEHASDSGLGTSISGSKYDDVRTQRSSVRESINTSITSAHSAVTRSFSALGASEEKHTLGEYACKQIHDTIIKPILAEESLKDFHPLIKDVPRRIGEKNICNLRDLEKTLIFLAPELSATAASYLRFCERSIQLLHTTVEYLSEQDQRLPSDRPYTNNYFLDLIEQIRRYAAIMAATRHKQEKGEELDDMDYSRYDYNQVAAFLFTRSTNQALLSDEKITLRGGLSHDGRPVELVREKNGKVMAIAEGASNAYKGLSKRALSDDEMDDDEVTRSMARRRKSEKPGDVMHTCRDCKKEFKRPCDLTKHEKTHSRPWKCTEEKCKYFDLGWPTEKERDRHMNDKHSAAPAQYKCLYPPCTYASKRESNCKQHMEKAHGWQYVRSKSNGRKKAPTNNDCSPATPLTPFIGTPQSATLTTPITPFAPSPQVPMIDTFDYYGFGTPALSVQGFQDDFRRDSVTTDGSHFTYSSGHSPTEPTSFDDAVTPEDATINHNVVFNDCGLGANFNTGFQQPTPALSTTFDYEPLPFTANSHLTAGLPHLSPMGQPDVTLFSPQMHMDEGFGDMDMDMQTFSRPTEDFTLFDTAPPSTMPFNNTANFFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC59</accession>
    <name>A0A9W8YC59_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC59"/>
    <dbReference type="OrthoDB" id="3794120at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="427" mass="48025" checksum="844991BB24F6596F" modified="2023-11-08" version="1">MMDRATTITPSAPASRASTTALESNENEAKMTPPKSTSQSSDIDCILVGAEAQVHTDNKKELSGDFKPLQTLMDVAISVEYGERLSCQVTIHEGLLRCHSKLFEQLCARAKLLRGRYIQSDAMMDRLALYMFPETTTEQFENDDMGAKVIPLILHIFEKHPLLEYQAETRKVIVDAVEDQITKKNTRVKRDSKEWSRNMSVRARIPLLKSHAVQTVAQSLYVRLHQVKKMEAQKAKDDSVKAIAQKRLILPGIEETAILSFVQWIYQRPMQCEEPDMLYAVLNLATKLGVEALAETCLSKLYNAAADSIQHARANAVPIHTLLGYGSGAADPVLEIVFINVFKDKSPPKRLLKLVIDTLAHCLDFELWAHLRELIGHEMALQLVEGMINSREVKIEHFDQTSFNSESDRLVDEKSAFMANNGCGFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCH4</accession>
    <name>A0A9W8YCH4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_002655</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCH4"/>
    <dbReference type="OrthoDB" id="5296at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052184">
        <property type="entry name" value="SDR_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45458:SF1">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45458">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE SDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4373916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="27718" checksum="8C304FF66B550C9F" modified="2023-11-08" version="1">MSQTILITGASSGLGLAFTKHYTSQPSTRTIIAVDHERFPTENYFDDVGYTPGLLHFHRLDITYSLDFEIVPDLIGDTPVHLIIHCAGIRGLVPDIVEEDKGDVAAAETLEVMDKQTMLKTFETNTWGTFNVIRSFLPNLKLAAKENQQPKVIILSSRMGSISSNDGGGGYAYRASKAALNAVVKSFSVDVPDVMFLLLHPGRVETGLVQWREEGAISVEESVRDCLKVIEGLGRKESGDFVDRFGERIDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCT1</accession>
    <name>A0A9W8YCT1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Auxin efflux carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_003227</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCT1"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31794">
        <property type="entry name" value="AUXIN EFFLUX TRANSPORTER FAMILY PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31794:SF4">
        <property type="entry name" value="AUXIN EFFLUX TRANSPORTER FAMILY PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="360"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="431"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="172"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="178"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4372936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="459" mass="50315" checksum="4CFCA679911C8648" modified="2023-11-08" version="1">MPMSGLLSSFVAAVQASLSVLLVISYGGIAAKLGLLDSSNGKAISKICVKMFLPALLLIQIGTEVHLGSVHRYLIVLAWAFICHLISFAIGILAHLMFGLPDWVTAAIMFNNTTSYPLLLIQSLNETGILVDLIVGDDTTKAMIERAKSYFLVFATVSSCLTFAVGPRLIDSEHAPESDDDKTLDEEEDDNEGGNGRDIEDGARALPPNEHTRLLSPQPRQRQRSRHESVVAITFFPSAPKFTTVKRRPFYIPYPHWADLSPRAQWWLLFLYDFLNAPLLGALLGVIIGLTPSLHRAFFENTYSGGIFTAWLTESWKNIGSLFVPLPLIVAGISLYTSYQNSKQGDDNGSRTSIPRATTAFILVVRFIIWPIFSIGVIYAVVKHSGRHGILGSDPMLWFAMMLMPTGPPAMKLITMVQVSDAGVDDERRIAKILTISYIISPVLAVTVVGALRASQAVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCW1</accession>
    <name>A0A9W8YCW1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Spliceosomal protein DIB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TXNL4A</name>
        <name evidence="8" type="ORF">N0V83_003272</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Essential role in pre-mRNA splicing. Also essential for entry into mitosis (G2/M progression) as well as for chromosome segregation during mitosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the DIM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCW1"/>
    <dbReference type="OrthoDB" id="147752at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02954">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000004">
        <property type="entry name" value="Spliceosomal protein DIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004123">
        <property type="entry name" value="Dim1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052:SF5">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEN 4A, 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02966">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017199">
        <property type="entry name" value="mRNA_splic_U5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01410">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="39"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR017199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR017199-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4372981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="143" mass="16665" checksum="9A6288F759D301D0" modified="2023-11-08" version="1">MGSVVLPHLRTGWHVDQAILSEEDRLVLIRFGRDSDPDCMAQDEVLYKIADRVKNFCAIYLCDLDEVPDFKAMYELYDPCTVMFFFRNKHMMCDFGTGNNNKLNWVLEDKGEMVDIVETVYRGAKKGRGLVVSPKDYSTRYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDI0</accession>
    <name>A0A9W8YDI0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Endoribonuclease ysh1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">YSH1</name>
        <name evidence="15" type="ORF">N0V83_003590</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the cleavage factor I (CF I) involved in pre-mRNA 3'-end processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF2/YSH1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDI0"/>
    <dbReference type="OrthoDB" id="10249535at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16292">
        <property type="entry name" value="CPSF3-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000001">
        <property type="entry name" value="Cleavage and polyadenylation specificity factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10890:FF:000004">
        <property type="entry name" value="Cleavage and polyadenylation specifity factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021718">
        <property type="entry name" value="CPSF73-100_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF11">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11718">
        <property type="entry name" value="CPSF73-100_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01098">
        <property type="entry name" value="CPSF73-100_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="12">
        <location>
            <begin position="36"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="13">
        <location>
            <begin position="263"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA 3'-end-processing endonuclease polyadenylation factor C-term" evidence="14">
        <location>
            <begin position="538"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="687"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="745"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4373296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="831" mass="92329" checksum="66242A3613BAD56A" modified="2023-11-08" version="1">MATKRKAAAMAPEDDDPIDPSDELMFLCLGGGNEVGRSCHIIQYKGKTVMLDAGMHPAYEGLAAMPFYDEFDLSTVDVLLISHFHVDHAASLPYVLAKTNFKGRVFMTHPTKAIYKWLIQDSVRVGNMSSNSETKIQMYTEADHLNTYPMIESIDFYTTHTVSGVRVTPYPAGHVLGAAMFLMEIAGLKILFTGDYSREDDRHLVSASVPPGVKIDVLITESTFGISMHTPRVERETQLMKAITDILNRGGRALLPVFALGRAQELLLILDEYWSKHPEVQKIPIYYNSSLARKCMQVYQTYVSAMNDNIRRLFTERMAEAEAAGDTGRRGAWDFKFVRSLKSLERFDDLGGCVMLASPGMMQSGTSRELLERWAPDPRNGVIITGYSVEGTMAKQIVHEPEQIPAIMTRASNTARRPGQRENEQTMIPRRCTVQEYSFAAHVDGKENMEFVQEVAAPVVILVHGEKGNMTRLRSKLLSFNAQKPVPTKIYSPANCEELRIPFKTDKVAKVVGKLASIAPPMRHPEGEGDEDMEESGEGEKVDMITGVLIQNDFKISLMAPEDLKEYAGLTTTTILCRQHLMLSAAGIDLIRWALEGTFGAIKESAMTDAAVDGKEVNTNGNGVHKDDADEEVSRTETKFTVMDCVNVLIKNGGRVEVEWEGNVINDGIADAVLAVLFTVESSPAAVRQSSKQHSHTHHTEKSEAQLTHRIKQPHPHRTTDPSTRLARLFLFLEAQFGEDAVRPITLPRNTTSSTTEVSDDDSAISPEDKQELKRLHNLGIPVPGIEVKFDKWVAKVWLEDLDVECANNALRARVKAVVERGIETVSGLWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDI1</accession>
    <name>A0A9W8YDI1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">SF3a splicing factor complex subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PRP21</name>
        <name evidence="9" type="ORF">N0V83_002845</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDI1"/>
    <dbReference type="OrthoDB" id="447637at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.790:FF:000001">
        <property type="entry name" value="Splicing factor 3a, subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.790">
        <property type="entry name" value="Surp module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045146">
        <property type="entry name" value="SF3A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022030">
        <property type="entry name" value="SF3A1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15316">
        <property type="entry name" value="SPLICEOSOME ASSOCIATED PROTEIN 114/SWAP SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15316:SF1">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12230">
        <property type="entry name" value="PRP21_like_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="SURP motif" evidence="8">
        <location>
            <begin position="129"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="346"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="346"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4374106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="514" mass="58193" checksum="0D8B8F93E3FB3E5C" modified="2023-11-08" version="1">MANPNGEGAAPGAYELSVKPPSNVVIPPPNVRENVAKTADFINRRGDTQLPGLKQRVAGDPKTSLQFVLDDDAYNAYFLWYLQQLKEGHGPAANQSAQPVVQKPKGPPEPPKFHFSARMPNISAKDLEVLKATALYTARVGENWLKELRNRESGNFQFDFLRPNHSFFQFFRSLVEQYKILLEEEATVEARIEEVQRNSKNRFHVLDRAKERSEYVKYVTAQKEKEVKKAEDEHREFASIDWHDFAVIATVLFDEADDAVDLPAPALLNDLQSQSLEQKAMVSLSTRRLEEAMPDENTYYNATQQHVPPPPVQPNYAPMAPPVQAPYATYAPPPQDYRTPAQIAREEDQARLARERQAESDQRARAQAATRGAPGRIVTDYVPRAGLKKSNVQMSICPNCKQSIPANEMDEHLRIELLDPRWKEQREKAEARYSTTINTADVANNLKRLASARDDIYDGATSLPISAEEEARRKRAALSYDGQPDPAKDAARLAQMQSMNVQEQLRRIQEKHRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDQ3</accession>
    <name>A0A9W8YDQ3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_002091</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDQ3"/>
    <dbReference type="OrthoDB" id="4790878at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4375012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="309" mass="35380" checksum="CA083BF813E41CED" modified="2023-11-08" version="1">MAPTQAYKGTRPTLLTLPGELRNRIYRYALSCDTLKVILPWEDKPKPRPSFNSRLRPPFKSRSAVSKLTLMDAAVDDIWGVVEFNQLKFACRQLYAETAGLELQFNNIRITRRWGFENEAGKQFLQIADQVKGKEHWLSGSIITLEDVCSEPRSSEPAGAFDHFFPDTSQTIAKIGRWCRQHASVRVDVNLTPVDLCGRCENCLRFLMTSSAALAHILWRGQGLIDVLPSGLIPIALRERLQIMDSWVRQADTNDLQVPTLRFRPPIRKFNDKTIKGPIAQLAVDHPEWKDVMEEKWPEQVKRWVEDGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEB7</accession>
    <name>A0A9W8YEB7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003592</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEB7"/>
    <dbReference type="OrthoDB" id="3800294at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4373298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="445" mass="50534" checksum="AC2609A0474FD82B" modified="2023-11-08" version="1">MESISRSLILRMLCDKDDDEDSTQGIYHCCFQKSEVVSASAGDKAGFSGEDANLGDVRAWLDSECAKELEKSCWQDEETEAVVVMTEVKSLGVDTSILNRIHEGRGPRDAAFEDLLAPLRLHSPAGPSSREETYDDMVEVLLAWNQHVTHAYDNTEGRELLIASLHERLKAVNKIHEMIKDRRASEMYDRIGHKGILEPSARHILQALHIYRPEIKRFKNQDPFDSLVMLWANTRPEIPDIKIDDEMRYLSFKVLVAEQGLAQRYADESFRREIKRKRDSARQRKDKPSYLRLQEEKEHFEKAAAKQALQSTHSKIFYARSELRKLGLQDLQALTLLQASQKLTTRTTFSRIPSWTVQPNCTNLYASCAQADFALDLILHHCLPALLTTTSTASTTISSLLAIHATALTRALKLTGHDLPSNVDAVTKRRIVEQRKSEGVRRRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YES5</accession>
    <name>A0A9W8YES5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_002420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YES5"/>
    <dbReference type="OrthoDB" id="5354164at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4375334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1010" mass="113561" checksum="7FB334C1DF8E9421" modified="2023-11-08" version="1">MEAPVEFSGIVSALSARRRVEAEEGPSHKTARRLGALFEDLVPSTPKLISAYGLRMSEIMNTCGVNDIGTGQLGPFEPYVGADGTALWAAATSGISALGVYLLSCLLARAWDAKTATAIWVELVAARRNEVQEGLQNNHNISASSVVGAYQDITRKDLALWDSSARAWLRRADKAKGWSQCQLSLIVKNIKTPIAGGPSTYDKVINVWKRSMLAVEQFLGGKPQNITEGSVFLAFSAWHLYPDLIVLGAEPKKIEFEDKLIPPAGVGTVGVESRSSEREGVQWSLALSHLQYYGDPVVVRSNQDYSRLTFAQLQIVMLGGLLGRWKISSRDYLLVAEWFKLLWSRMGMSEEAIENGHSPEFGWLLHFVVAANRLLASNDQERQQNLILLKHGSRRAQRFLSDDNCNQSPLFGLLNTLTACGLQENLDIDSGIAYLRAVAEKLGLKRSDAIICYAHDASYKNNPASVNYFELATVQSLEMEIHARWICPNGEATSFPTSGTNVNSHGNLWDERSTPDDRIRYIFDQGEKCNSYATGPSYTSRTNRFSFFNWLNPPPIYLHDRSITPHDQDDAIHRSKKAKVNVSFDAIVGDWRLGLFLRNEARKGHDPLQPYQKKAKFETTKTESVSAAVQNFRHTKLVPERLRDYICSIIHVSKEQSEYNRTKAPGVSLFSNAHRFDPGFSRSIYAMDSASKVYSHLVQATISLKLTLQPLSSSAWFQCLFTRWEPDYTNMNTWSNQTPLLTPLQPPLPDRDETFSCIALFDSGNVDLSPEDFKLSFALCSEDTMYVPAVVLSDPFTRVPDYEMKSITGNIGRQGISILVPPFEPRIRELSNSYNLVPHETYDFKREDNFRETSLHLSFTDWTSPLATEDSRMIDHDAQVVEAVISVHDRGRWVADINILDVNFRDMIRFKPATSCKGNHDKSYDFDYTSIDSWEELLDEPSSVGFFRAHRNWAARLAAVSILSRNEAEGHNFGVLGPEPFCLKCLEEEHENPSWNMLDYESTLPSFCID</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEU8</accession>
    <name>A0A9W8YEU8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_001043</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEU8"/>
    <dbReference type="OrthoDB" id="20872at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39608">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G08640)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39608:SF2">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="6">
        <location>
            <begin position="18"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4375767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="172" mass="19336" checksum="EB7450740421A26C" modified="2023-11-08" version="1">MARTRLSNRNWRYSVAACNALTWISSAIVVGITSHFINDYPHDQHLIYELVISAIVLGLWLPSFILPIVTRYKFYYGPLNFVFSYLWLTAFIFAAQDYNESQCQLNAPFGGSCSLKLTNEAFIFIAFFFSLVASLIDAYAYHTARVIAAEPVHPEKPDHDNAPAVTPAHAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFC7</accession>
    <name>A0A9W8YFC7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 18">Serine/threonine-protein kinase RIO1</fullName>
            <ecNumber evidence="4 18">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">rio1</name>
        <name evidence="24" type="ORF">N0V83_001260</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 21">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4 18"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFC7"/>
    <dbReference type="OrthoDB" id="205248at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05147">
        <property type="entry name" value="RIO1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000403">
        <property type="entry name" value="Serine/threonine-protein kinase RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051272">
        <property type="entry name" value="RIO-type_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018935">
        <property type="entry name" value="RIO_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017407">
        <property type="entry name" value="Ser/Thr_kinase_Rio1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45723">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038147">
        <property type="entry name" value="Ser/Thr_PK_RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01245">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 18 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 18 24" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 18 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 18" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 18 24" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO kinase" evidence="23">
        <location>
            <begin position="128"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="465"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="22">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="22">
        <location>
            <begin position="469"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="499"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="22">
        <location>
            <begin position="517"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="19">
        <location>
            <position position="317"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="19">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR038147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR038147-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR038147-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR038147-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ4375980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="539" mass="61601" checksum="7AFC0E470035AD43" modified="2023-11-08" version="1">MASTQEPEPLNQQPEGLQHPEPHPATETPAKEDDDDIEDLFDESDDEIVPELISGDSHDFTKAYNRQRKLNDTSIPVDHKPKSNPQKPSANTNSSIDDQVASLAKHANKIRLTDKFSGATGGNARDKDKADRATTEQVLDRRTQMILLQLINRGSISEIHGVISTGKEANVYHAITEPSDADADADPIHRAVKVYKTSILVFKDRAKYVEGEFRFRQGYNKSNNRAMVKMWADKERRNLARIHDAGIPSPEPIALRNHVLIMGFVGDRKGKAAPRLKDVRFDGLTTEEEDAKWTDLYVQMLSYMRILYQTCRLVHADLSEYNVLYHEGRQWIIDVSQAVEHDHPRSLEFLRMDVKNVSDFFRSRNVETLSERHAFIYVTSETGAKDIKDQEKIEEQVRALLGKTPLTEEERKREEEDDEVFRNQYIPQTLDQVYDIERDAELVNKGEGSDLPYQALLADKVVNNNESDASPEDSDSESDGSEVDQSLFDKGPSRGKKHMDKDEKLAHKKAIKEEKREKRKDKMPKHMKKKLVSQSSRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFK9</accession>
    <name>A0A9W8YFK9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Concanavalin a-like lectins glucanase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001381</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFK9"/>
    <dbReference type="OrthoDB" id="2332199at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="235" mass="25899" checksum="75E292A6CEDD0E2D" modified="2023-11-08" version="1">MGNTQSTVRYLAPASFLYDFAAQQYGIFSSPNMLDIHNQNLAAFSPYPYFIGAFFFPQQLFQLAWLWKLWRQDGNQQECEMMNKFAWVYSLGNVCIGTWMFFWNSNNLATSNVFVIINIVAQVAYIATALEPLDTRSTPNLLTHIVAKTFAGIGVLDLLHNTSAAYFKGVPPSSLVQVATGVGFAAAASMSDWIFGGCLVYDLVALAVGQEGNWSRLLGGFAAMTAGIVGFKNFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGH3</accession>
    <name>A0A9W8YGH3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase M24 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGH3"/>
    <dbReference type="OrthoDB" id="9995434at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050659">
        <property type="entry name" value="Peptidase_M24B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46112">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46112:SF2">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="1">
        <location>
            <begin position="214"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="442" mass="49571" checksum="9A88B674725881D0" modified="2023-11-08" version="1">MAFKNLLSSRDRNSNDLRSEEEELFRRCSIDSYLATGLRFLESARRPPLEDYILRRNNLAKALVAEGVDAFVVEPGYTFSYYANITQSEWEPWEPEERPFVMIIRPETLPDGKIVANTSFLVPHFEETRARLLNMPFREDLHTVTYEEHWNFYTTLYQSEIWGDTLSPIIMVDEELRDFIQRGLASNGFTVVGLAGEVEVVRQVKTEREIGILKAVNTGTAEAIRAMRSCLYQDVRERVVAEVLDDTMRAAGLEPFFNIVEFGKSAALPHGGYDGTRKLKPGMFVLIDVGAHLYGYSSDICRTFFPPFTPNPPPSTTHNMTEQLDVWQLVLDAQTAAVKAMVAKGTAAAVDIAARDVIEAAGYGDYFTHRLGHSIGIKAHESPYLNKGNFGAVLRPGMVFTAEPGVYVLDKFGVRHEDVLVVRENGDAENISGGFAKSPWDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGI6</accession>
    <name>A0A9W8YGI6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61</fullName>
            <ecNumber evidence="2">2.1.1.220</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">tRNA(m1A58)-methyltransferase subunit TRM61</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TRM61</name>
        <name evidence="12" type="ORF">N0V83_000062</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.220" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGI6"/>
    <dbReference type="OrthoDB" id="1925287at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160107">
        <property type="term" value="F:tRNA (adenine(58)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049470">
        <property type="entry name" value="TRM61_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014816">
        <property type="entry name" value="tRNA_MeTrfase_Gcd14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133:SF2">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE CATALYTIC SUBUNIT TRMT61A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08704">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51620">
        <property type="entry name" value="SAM_TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 C-terminal" evidence="11">
        <location>
            <begin position="118"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 C-terminal" evidence="11">
        <location>
            <begin position="214"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 C-terminal" evidence="11">
        <location>
            <begin position="333"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="293"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="404"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="317"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4377239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="494" mass="54314" checksum="7D25A7B11C11D2CF" modified="2023-11-08" version="1">MAPAPSPFLDPGTHARANSLGILQLRRDHLIPAVLSPNADSDYAEGKVENTRFGSFPHSTLVDQPWGTQILASVVDTGSRGKSKKRKRDTKDEGAETPVTEVEEVKKDIVKAAVASSGFAHLIPPTPETWTLSLPHRTQVVYTPDYSYILQRLRVRPGDHIIEAGAGSGSFTHAAVRAVFNGYPGGAEDASNGTHDDDEESAVGISKKRKRKARHGGVYSFEYHEPRAKQLQVEIKEHGLNDLVTVTHRDVYNDGFCLADDDSNLPDADAIFLDLPAPWQALKHLTRSPPSSAALKSVAASSDPSTPSNEEAPAPPTTDSSSQPFRSPLNSKSAIRICTFSPCIEQVTKTVSALRSLGWLSIDMVEIAAKRLEVRRERVGLQEEGVRGGNASAATVQEAVQRLRDVEGERLGRKGEGKGKKEKEKLTKEGLPPSKHDRLEKTKRDLENRTLFKEGRLVHRTEPELKTHTSYLVFAVLPREWSKEDEEKASKKWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGY8</accession>
    <name>A0A9W8YGY8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_001330</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGY8"/>
    <dbReference type="OrthoDB" id="2130629at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000447">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF11">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="345"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="398"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="435"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="474"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="40"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4376049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="522" mass="57362" checksum="798046A60DBED930" modified="2023-11-08" version="1">MADPTMSDPKIEEGCPSPTRGSYSPSELERLGRERPEVFTSLWMELGFCFALLGSMFVAEYFISGFNILLPDLTEALHIPSNAQTWPASVFSLVTGAFLLPFGRLADMFGGYLVFNIGLGWFLIWSIVAGFSQNYVMLIVCRALQGFGPAAFLPSGIMLLGSIYRPGPRKNLVFSLYGAFAPVGFFTGIFFAGLSAQFIKWGWFFYIGSIILAIVGVASYLCIPNRPEDKSDKDIIMDWWGTATVIPGLVLIVFALTDGSHAPKGWATPYIPVTFVIGWLFIGAFIYIEGWVAEQPLLPPDMFDVKGMKPLALALFFQYGTFGIFLFYASFYIEKVVGAGSLLTAAWFTPMCVGGLFLATIGGLILHLLPGRILLLISGTAYILCALLFAIIPVEFNYWAYIFPSMICATLGIDVTYNVANIFITTSMPKARQGLAGAFMNSLLFLGIAVFLSFADLAVSQTENRGERKSYKVAFWLATALAGAGLIIMFFGVRIGKAKSDLTVDEREELEQELTRRATDEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH43</accession>
    <name>A0A9W8YH43_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lytic polysaccharide monooxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000438</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH43"/>
    <dbReference type="OrthoDB" id="2342176at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36182:SF2">
        <property type="entry name" value="LYTIC POLYSACCHARIDE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36182">
        <property type="entry name" value="PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G10930)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040792321" description="Lytic polysaccharide monooxygenase" evidence="2">
        <location>
            <begin position="19"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="248"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="396" mass="40063" checksum="79D238C0CDFDBA2A" modified="2023-11-08" version="1">MLFSQATLVLALAASATAHMRLKYPVPYGQDTLTSSPLAPADFPCKQRTGVYDVTEMNQWNAGETKTIAFTGSAVHGGGSCQFSITTDPKPSEQSQWKVIESVVGGCPSNVSGNLPEEVPSGSGDGAATFPVTMPSNIPDGQYTFAWTWLNKVGNREFYMNCAPVQVGSGSGSGSTESAASALASLPDMFVANLPNTECSTSENEDFNYPDPGKSLIEGNGASLGDTLTGAGCSTQTKLGAGNGQIGTPTQPQTSAQASASGQPAATSAPASSAAQESSVASSVPGVFAPGASSAAAEPTTTAAAEPVATAAPAQSSSPDSNTTTPSSDCTPCTTDGAVVCIGTNQFGLCNRGCAVAQDLAAGMACSNGVVVGATKRSFSFPRAHLHRRHGSSRFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH78</accession>
    <name>A0A9W8YH78_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amidohydrolase 3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000288</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH78"/>
    <dbReference type="OrthoDB" id="3501663at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01300">
        <property type="entry name" value="YtcJ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033932">
        <property type="entry name" value="YtcJ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22642:SF20">
        <property type="entry name" value="AMIDOHYDROLASE 3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22642">
        <property type="entry name" value="IMIDAZOLONEPROPIONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase 3" evidence="1">
        <location>
            <begin position="58"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="544" mass="59302" checksum="5CAB3AC2CE84598D" modified="2023-11-08" version="1">MAATQNVTLFTNGHFFRGAGLKSETDFHSALVVQDGRIAFIGSSDAHEVQSYQDNGAQVEDLGGKHVLPGFIDGHMHFLLLGQSLNKVQLDGSKDLAEIRSRISQYARTHPDKERILCSGWMHSMTNGEAKANMLDDLDSRPIYIDSKDLHSCWCNSAALKEMGVHEMENPAGGTIERDEHGNASGLLSEACILLIVWPYLANVLPLEDKMAALRAAIHAYHNVGCTGCIDMAMDENAWEAILALRKVEGGSLPLRVAAHWCISPGNGEEHRLQQVERAIELNRQFNKETSPDLRIVGIKIICDGVIDACTAALAEPYTSNGHFEGPIWTPEMLKNVVKKACDAGLQCALHAIGDQAVHNAINVLEKYGRPGQRHRIEHLELTAPEDAKRLGKLGITASIQPVHSDPAILRAWSKLLGPERLKRAFAYSEFADHGAHLAIGSDSPTAPHAPLPNLYVATTRKSARQPDGGDEPVNGNFRLGLAQAISAATAGVAYSCFAEDRVGSLEVGKMADFCVIETKWKSDELLGARVTETWFSGRKVYES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHK6</accession>
    <name>A0A9W8YHK6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000594</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHK6"/>
    <dbReference type="OrthoDB" id="3918601at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38794">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38794:SF3">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="2">
        <location>
            <begin position="8"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="205" mass="23188" checksum="3762DC9ECE27DACD" modified="2023-11-08" version="1">MGLSATTIVLASDGLGDPGFLALDRADVLMKGYYASEFLYISAICFTKLSLLVLFYNIVVVQRFYRRFVLVFGVVISAWTIASLLAVAFQCELPRPWETMTLRCFNSRIFWVVYCIVDMSTEISIIMLSVNLVAYLKVRFSSKVAVVACFAPRLLVISAALIRLIWLYPITPHSDPQYRLWLPSIMTQVQTDEPIDTTEALHPTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHW9</accession>
    <name>A0A9W8YHW9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_000759</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHW9"/>
    <dbReference type="OrthoDB" id="444631at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF146">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="32"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="357" mass="39936" checksum="2572D98A64DE6C20" modified="2023-11-08" version="1">MAGAAKSPISRDELVRTNSAMLILTSVFVLSRAALHISKRKPLEFSDFFIYFAYALYIALWSCYISVVPPMFKVYAVLGGEIKPYATMMDDAATMLKLITAGQMCFYTLLFSVKMSLLTLYCKLLAGVAGIYKKIWWGILVFCVVAWLGSVFSSIFTCNNLKEKFSKGKCGGTANEDQRIIFSLYFAYSVDVATDLAIMFLPIRLTWNLQMPKAQKLGVFVLFGSGFLCIAFATLRVVQLGVDGRGKTTTPEPKWMLLWTVLECSIAIIIGCSPAFAVLIRKRMNSSKKASYNAQGYLKQSTEENIKMKNLVSSNGRPKRDMYWDDTHSSQEELAKNAGHIVVKTTVHQDDESISRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YI77</accession>
    <name>A0A9W8YI77_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000631</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YI77"/>
    <dbReference type="OrthoDB" id="5405297at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="465"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="516"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="553"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="566" mass="62785" checksum="B8A300A5495DA8AD" modified="2023-11-08" version="1">MGFFKRFRRQKSPKKNASLPRNDFYSPATYAYHGPDQTQRLPDKILRRIFEEVCPHSADETLDGSEDSGNDGCMSCDMRDLAHCALTKRQWYGVAAGLLYNSIRIDAVHYCEIEEVIADQRRRKSRNGDELDAPTTRLQQLCQSVRGNQYLGQRVRFIKLPYMTRESCKADIARVVSGCPNLEFIDLPDGFYNGDASCQLLRQELQARCPHIRKMKYNEGGEQSLESLLHGYWQELLVLELNRIQIEPSILRQVFGMLPRLSELIISGVSWLNDSTFHNAPGLPAFPALDTLKLTKVHGVTAEGLALHLSSPMCGNRLRTLNIKDCSGIPASSLHIILQSATNLRTLEYTATVSASLPLDPLPPLTSHTLHKLNFEIISSSSNQLYPPGASYYQYLARSLMANCLPGLRQLYVRDPEFPEILTLAPPIRPFSEAPPPMFNQPLEVYSKGLDELEWIFTSIIPPEASGRRPSVSGGRPVSSYSAHKGLGPQWGGEARKSVVVPNGFGGFLAVPAEEARPRSAGNLAPSGGMGHGYSNSLGGNGPGPRASWMSHAGREKRASRADLWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIP3</accession>
    <name>A0A9W8YIP3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIP3"/>
    <dbReference type="OrthoDB" id="3684248at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="283" mass="32608" checksum="1612604683D6308D" modified="2023-11-08" version="1">MNLFNTAFDYIMDTATDFLKKKPAYNPAKPNRVDADTSLLQYHQTGTGPKPAPASTSALPLRTLRATNLFSDLHAAQTHAYYEEIDSIPAVSVGLKSAFSAGHWYVVHPVTAVDVLFLSTRVLQDLAKWLEKDCPNELFEGEGAQVRDFKGVRSETAENVKRELQKREERGDVGKEEGYTGIGRRLGMREKKRRRRGSYDQDEKDEMKDDMDIDWRATDDMRGQKRWHVSYRKEDNTLYVQVVRNKRVCRNTRKMNVSISLGSFRAWMLMENVRETRDGTYAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YK11</accession>
    <name>A0A9W8YK11_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SEC4</name>
        <name evidence="12" type="ORF">N0V83_000516</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Protein transport. Probably involved in vesicular traffic.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YK11"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01867">
        <property type="entry name" value="Rab8_Rab10_Rab13_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000363">
        <property type="entry name" value="Secretion related GTPase srgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4377687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="206" mass="22805" checksum="CFB8155427BEF4F4" modified="2023-11-08" version="1">MAGSRNYDFLIKLLLIGDSGVGKSCCLLRFSEDSFTPSFITTIGIDFKIRTIELDGKRVKLQIWDTAGQERFRTITTAYYRGAMGILLVYDVTDERSFNNIRTWFSNVEQHATEGVNKILIGNKCDWEEKRAVSTEQGQALADELGIPFLEVSAKSNINVDQAFYSLASDIKKRLIDTARTDQTVQPRVDVGGTDGGNAGLGGKCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YK20</accession>
    <name>A0A9W8YK20_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Riboflavin synthase</fullName>
            <ecNumber evidence="4">2.5.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RIB5</name>
        <name evidence="11" type="ORF">N0V83_000531</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the dismutation of two molecules of 6,7-dimethyl-8-ribityllumazine, resulting in the formation of riboflavin and 5-amino-6-(D-ribitylamino)uracil.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.9" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YK20"/>
    <dbReference type="OrthoDB" id="10258924at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004746">
        <property type="term" value="F:riboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00402">
        <property type="entry name" value="Riboflavin_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000004">
        <property type="entry name" value="Riboflavin synthase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000006">
        <property type="entry name" value="Riboflavin synthase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001783">
        <property type="entry name" value="Lumazine-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026017">
        <property type="entry name" value="Lumazine-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006767">
        <property type="entry name" value="PRK09289.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00187">
        <property type="entry name" value="ribE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21098:SF0">
        <property type="entry name" value="RIBOFLAVIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21098">
        <property type="entry name" value="RIBOFLAVIN SYNTHASE ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00677">
        <property type="entry name" value="Lum_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000498">
        <property type="entry name" value="Riboflavin_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51177">
        <property type="entry name" value="LUMAZINE_BIND"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Lumazine-binding" evidence="10">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="Lumazine-binding" evidence="9">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="Lumazine-binding" evidence="9">
        <location>
            <begin position="104"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Lumazine-binding" evidence="10">
        <location>
            <begin position="104"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4377702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="236" mass="25149" checksum="CADFA3DA6EEC4A60" modified="2023-11-08" version="1">MFTGLVETIGTVAALEALDKSASGGGGTSLTISGCDEILGDAHLGDSISINGTCLTVTEFDKSSFKIGCAPETLRRTNLGSLKQGSKVNLERAVSASTRMGGHFVQGHVDTTATIQSITPDGNALTFRFKPRDASVLRYIVEKGYVTIDGASLTVTRVQDGPDGWWEVMLIAYTQEKVIIASKQPGEDVNVEVDQVGKYVEKSVAGYFERSANGEFAILDKMVAKLVDERLAAHGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YK53</accession>
    <name>A0A9W8YK53_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000815</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YK53"/>
    <dbReference type="OrthoDB" id="5413997at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="291" mass="32221" checksum="7E63A884869E8112" modified="2023-11-08" version="1">MNPLRLVTEKCFDDDTGEQVQVLHQGTQPVFLDRSYIQFTTSDSRQSYELPFIASSLQPGRKYKLRFTPTTSISHWPASAEDALNSLAGGASSSSVSTSDIPAPSTQNILWEAAAGNDTVVFETRSSRPSTPNVTVSLSAPSTYSLSKPFTFSLTFSTDTACPITVLADRARVKSLYSDVKILDATTRVQLAPDVIVCRDEDEHQREEFSRLEGTYTEHRELDLNGPLWENVQLEVGKEYILSHLGGTWWWTEDSIDEVMTYLESKSSIGLALTETIQFASAGEVGFKVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHN1</accession>
    <name>A0A9W9CHN1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Protein ROT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ROT1</name>
        <name evidence="13" type="ORF">N0V83_010081</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Required for normal levels of the cell wall 1,6-beta-glucan. Involved in a protein folding machinery chaperoning proteins acting in various physiological processes including cell wall synthesis and lysis of autophagic bodies.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ROT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHN1"/>
    <dbReference type="OrthoDB" id="5327821at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006458">
        <property type="term" value="P:'de novo' protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019623">
        <property type="entry name" value="Rot1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28090">
        <property type="entry name" value="PROTEIN ROT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28090:SF1">
        <property type="entry name" value="PROTEIN ROT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10681">
        <property type="entry name" value="Rot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017290">
        <property type="entry name" value="ROT1_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040722303" description="Protein ROT1" evidence="12">
        <location>
            <begin position="21"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR017290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4362964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="241" mass="26954" checksum="CF1537093AA44992" modified="2023-11-08" version="1">MVSRIAALLLSGAALVQVQAQSLPTPTDLIGTWNSKANSTMTGSSFYDPVNEKFTEPKHPGISYSFSSDGNFEEAYYRAVANPQEPKCPKGIIQWQHGSFEKLSDGSLKLHPIKVDGRQLYSDPCSFKSAVYTRYNASETFKRYEVVTDAYHNIPRLNLYKFDGSPLMPLYLAMSPPSMLPTTTLNPLTTQTATANKVKRGELPLNHEVVYKRTPGATRADQWWWFGVFLTAGGSVLYFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHQ7</accession>
    <name>A0A9W9CHQ7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_010054</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHQ7"/>
    <dbReference type="OrthoDB" id="4426556at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000082">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF61">
        <property type="entry name" value="MULTIDRUG TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G02780)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="312"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="380"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="448"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="32"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="485"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4362937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="513" mass="56626" checksum="C848598BB9B11737" modified="2023-11-08" version="1">MKDGKEVQLHTWNGPDDPDNPFNWSLAYKWILTITVCFISILTGLPAGSYGAGNEYMASRFSVQDEPFPNLYWATCSWNIGAALFPLVFVPLTENTGRMPGYFVAYIIFELFLFGSAFADNFATLVVTRFFGGGASSVSINIVGGTISDIWKGEKARSLPMSLFGFTSVAGIALGPFIGSAIVQIHTNDIGLRERWRWIYYIQIIYNAALIPVFWLILTETRGDVILKKRAKKLRKETGREIYAESELEKPSIARQLKISFMRPTKMLLTEPVVTFFTLWISFAWGILFLFFSSVVQTFSTSYGFDTFQTGLIQLAITVGALIGTIINPLQDWLYLRTASKNPERPGKPIPEARLYTSIPGSLLFTGGLFMYGWTSRPEMHWIVPTIAVCIVGIGIYSIYMGVVNYLTDAYEKYAASALSAASLGRNIFGAFLPFASYSLFKDLGFGWAGSLLGFIGAALSVVPIVLLFKGRQIRAKSPFMRESTFDGDVPEEKKPGPAGVAGGPRGAVDDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHX6</accession>
    <name>A0A9W9CHX6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PWI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_010223</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHX6"/>
    <dbReference type="OrthoDB" id="6275295at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1390.10">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002483">
        <property type="entry name" value="PWI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036483">
        <property type="entry name" value="PWI_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052768">
        <property type="entry name" value="RBM25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18806">
        <property type="entry name" value="RBM25 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18806:SF4">
        <property type="entry name" value="RNA-BINDING PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01480">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00311">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101233">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51025">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="153"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="PWI" evidence="5">
        <location>
            <begin position="681"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="103"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="218"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="364"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="551"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="224"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="364"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="551"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4363103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="773" mass="86184" checksum="447324803FBE9E70" modified="2023-11-08" version="1">MYPFQGAPGYNGPPGYGSAPPGMAPPGMGESEDSDLFLMEYIYGAEASMELTAIIDPAPPGVAFGQGPPALGGPRALPPNWQPPANMPNFNLNAPVIRLGTQGGRAGAQDSPVGGRKDNASMPARRGLGMDRDGDRRDGPQQVIPPTREEIARTILVCNIPASLSDEDMERIMETAGKLARWTRGFNDSNKIQTFGFAEFADAQSLETAAEIFQDVRVPTKRQKPGEAKKEGEEEAKREDDEEVETTKLQIVVDNESIKYAEQWSKTRNEDENTVQFRLDTAKEALSQVLASFFNPPNAPLMEYGADTIMHDAPAQDGDDVEVAFVQLAPGEDELADIPAEMREVVAAEIAAFRDRSIQRDRERLRREEELEAEERRRSGRRVSPPPSAPTGPGGANGVPLGPRADRGLHGAPSGPKGSQFPRDYQAGVTFVSGGVSNGGGVYIKREDEDDSASDSELERRRKKKHDEEADAEYKKELARWLKLENRTVAGLQRTSDRMRDEKAEKAAARAAQVKQLAEFDDDKEVGTRRHLYYRDRGEYMREREKVREVEAKEDAIDRSQEQRELATQQKQRDQARGQADAFLDQQAEELMRSQQQREPAQFKISLGAAAKKLETAAAPRRTAADVENLLEDEELTEQPGVKKRTLIPINFDAAVRANLTQEEIEDAQRQLARDIPNDKEGLWKWPVSWEHLPEKNIEKDIKDWAANKVLELMGLQEEMLVDAIVEHLKNKGGPQALVENLEVALDEEAESLVKKLWRMVIYYSETEKRGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ61</accession>
    <name>A0A9W9CJ61_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Pectinesterase</fullName>
            <ecNumber evidence="4 11">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_008751</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in maceration and soft-rotting of plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pectinesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="4 11"/>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ61"/>
    <dbReference type="OrthoDB" id="2019149at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000014">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033131">
        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321">
        <property type="entry name" value="ACYL-COA THIOESTER HYDROLASE YBHC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321:SF57">
        <property type="entry name" value="PECTINESTERASE 53-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041014042" description="Pectinesterase" evidence="11">
        <location>
            <begin position="23"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase catalytic" evidence="12">
        <location>
            <begin position="43"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4365133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="339" mass="37503" checksum="E60D9908DF67BC48" modified="2023-11-08" version="1">MATFVSRLLIPIFFFCATFASASNYVRINPSRGSLIVDASRKHPGSFANISTAINSLHNTTDAQTVFIYPGTYSEQVYIPPHAGPITIQGYTTNAGSYENNKVTITGNLSRTTAGLANNDATATIRLWTSNVKFYNLNIANTFGYAATNGQALALSAQNTNQGFYGCSFTGYQDTIYANEGRQIYAKSYISGATDFIFGLRAAAWFDKVDIETIGTGYITANGRDAENNTSFYVFNEAKVTGTSGPNSTYLGRPWRPYSRVVFQKSYLGDVVKPEGWSRWDAVQSVENVFYKEYKNYGPGAMIRQRANFSSQLTAPIKASDIMGKRFEKEFWVDVDYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ83</accession>
    <name>A0A9W9CJ83_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008085</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ83"/>
    <dbReference type="OrthoDB" id="3795730at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="293"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="325"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="366" mass="39456" checksum="F95088A9FADBF5BA" modified="2023-11-08" version="1">MPIGIGVGVVALIALLIGLGVCFWRIRGKKSSNKSRELPVTGRKRSASHATQWSMDNDQKTLVASFPTSPYNAGFHEQHIMAPGVFAKTLERNASGVGNMGPMETEKSLPLAPTENPLPPAPTDNPLPPAPTEQKRYALNVNISKSTIFDEDMIRAVSPLQSIATPRERAPKYRFEEYLPPVANTPPISITLERPGSTGGKSEYELERFPNQETPTGNSATKDDGSHEGMHSQDPRETTLSTLESKAPTLSLPDFPPASPGLSFRSYDWYQDILDPRTSTLPASDSERKSTILSAPLSLSPRLSEVDPRLVPQPLSPGAPPPVDATGSGAASSSTNSNFRLSPTVYRTPSRTPDDSSLPSTYSPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ91</accession>
    <name>A0A9W9CJ91_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">thioredoxin-dependent peroxiredoxin</fullName>
            <ecNumber evidence="3">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Nuclear thiol peroxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Thioredoxin peroxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">DOT5</name>
        <name evidence="16" type="ORF">N0V83_008841</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peroxiredoxin family. BCP/PrxQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ91"/>
    <dbReference type="OrthoDB" id="338622at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03017">
        <property type="entry name" value="PRX_BCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000157">
        <property type="entry name" value="DOT5p Nuclear thiol peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050924">
        <property type="entry name" value="Peroxiredoxin_BCP/PrxQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801:SF23">
        <property type="entry name" value="PEROXIREDOXIN DOT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801">
        <property type="entry name" value="THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 16" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="15">
        <location>
            <begin position="70"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="223"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="229"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4365223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="260" mass="26976" checksum="F0E735F172BE2A93" modified="2023-11-08" version="1">MPVELRKRKEAPPAPVRPAKKKAHAKGKKPASEKTVVEQVQEAVADTVETVKEAVVGKTNGAAATAGGAPKVGDTVDLTSFGGEVETNDGKKTTFAKLVEESKGGVVLFTYPKASTPGCTTQVCLFRDSYTPLTATGYSIYGLSNDSPKANTTFKTKQNLPYTLLCDPAQSLISAIGLKKAPKGTTRGVFVVDKSGKVLASEPGGPAATVEVVRKLVGGNAEGVPTVGDVKKQEAKEDKEAAKTADEVADTAAKVDSNEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ98</accession>
    <name>A0A9W9CJ98_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Endo-1,4-beta-glucanase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">EGC1</name>
        <name evidence="6" type="ORF">N0V83_008145</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ98"/>
    <dbReference type="OrthoDB" id="9971853at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050295">
        <property type="term" value="F:steryl-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904462">
        <property type="term" value="P:ergosteryl 3-beta-D-glucoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000131">
        <property type="entry name" value="Glycoside hydrolase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000174">
        <property type="entry name" value="YIR007W-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041036">
        <property type="entry name" value="GH5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052066">
        <property type="entry name" value="Glycosphingolipid_Hydrolases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308:SF5">
        <property type="entry name" value="ERGOSTERYL-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18564">
        <property type="entry name" value="Glyco_hydro_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="4">
        <location>
            <begin position="67"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5 C-terminal" evidence="5">
        <location>
            <begin position="637"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4365526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="757" mass="85023" checksum="0B06BFC02D70D7BD" modified="2023-11-08" version="1">MTTSPVRLRIDGRTFRDAENREVILHGINCAADAKFPATPNQPSHVSDNFFDGDNVSFVNRPFSLQDAATHFSRLRSWGYNTIRYVFTWEAIEHAGPGKYDEEWIDHTIAILRRAKEYGFYIFMDPHQDVWSRFSGGSGAPLWTIYACGLDPEYFAVTEAALVQNTYPDPSTFPKMIWATNYTRMACQVMFTLFFGGRDFAPKAVIDGKNIQDYLQDHYIGACEHLAKRINQAGDLWHDPIVGWESLNEPNRGLISFQDITVIPSEQKLQKGTSPTAWQAILTGSGRACEIATWDFGGMGPYKSGTALIDPEGKTAWLSPDYDDTKYGWKRDPGWQLGECLWAKHGVWDPATDTCLKKDYFAKDPKNGATITYEYFTNNYWLPHYRKYSKMVKSVFPDSLMFFQPPVLEIPPSIKGTEDDDPNIIFSPHFYDGITLLTKKWNRVWNIDVFGVLRGKYLTPAFAIKIGETAIRNCFASQLRAIREEGTQYTGLHPCIFTEIGIPYDMDDKYAYRTGNYSSQIAALDANHFALEASAANGFTLWTYVVTNSHYWGDQWNGEDLSIFSLDDKPVPAGAFSPADSRASLDMESPSFSRAHSTDTLSVTPSNLRKTITAERMSSSRTEGDVRGLRAAEAFIRPTPIAVRGDISSYGFDLKNCTFKLALVAPSSTTEGAPTLCYLPEFHFPYGQTNVEVSGGKWTISSEESNGAIQQILRWWHAEGDQTITVKGVIRKAGSAIGTDEDEGYLQQCQKQNCAVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJE7</accession>
    <name>A0A9W9CJE7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2423 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007418</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJE7"/>
    <dbReference type="OrthoDB" id="4087970at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019434">
        <property type="entry name" value="DUF2423"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28219">
        <property type="entry name" value="UPF0642 PROTEIN YBL028C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28219:SF1">
        <property type="entry name" value="UPF0642 PROTEIN YBL028C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10338">
        <property type="entry name" value="YBL028C_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2423" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="130" mass="14331" checksum="B2AB08B0BC068AB6" modified="2023-11-08" version="1">MAKSARASSKKANRTKLRARVFGPVEKARAERLHAKLLETIQQPKPEKTDMDTAEDATTSEATKEDDFPKDMDVDGTATASAAKKSSKKDKGQSRKQLRRKPQNKISFPSSRGKGALKPYSGSRVGKRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJY4</accession>
    <name>A0A9W9CJY4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Beta-xylanase</fullName>
            <ecNumber evidence="7">3.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_008011</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-xylosidic linkages in xylans.</text>
            <dbReference type="EC" id="3.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the glycosyl hydrolase 10 (cellulase F) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.8" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJY4"/>
    <dbReference type="OrthoDB" id="3055998at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030248">
        <property type="term" value="F:cellulose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031176">
        <property type="term" value="F:endo-1,4-beta-xylanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000254">
        <property type="entry name" value="CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035971">
        <property type="entry name" value="CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044846">
        <property type="entry name" value="GH10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001000">
        <property type="entry name" value="GH10_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31490:SF76">
        <property type="entry name" value="ENDO-1,4-BETA-XYLANASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31490">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00734">
        <property type="entry name" value="CBM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00331">
        <property type="entry name" value="Glyco_hydro_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00134">
        <property type="entry name" value="GLHYDRLASE10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00236">
        <property type="entry name" value="fCBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00633">
        <property type="entry name" value="Glyco_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57180">
        <property type="entry name" value="Cellulose-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00562">
        <property type="entry name" value="CBM1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51164">
        <property type="entry name" value="CBM1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51760">
        <property type="entry name" value="GH10_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040931314" description="Beta-xylanase" evidence="9">
        <location>
            <begin position="24"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="CBM1" evidence="10">
        <location>
            <begin position="23"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="GH10" evidence="11">
        <location>
            <begin position="164"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="65"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="105"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="105"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4366375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="466" mass="48558" checksum="BC4AB7595EF9CB3B" modified="2023-11-08" version="1">MHFRGSSIYLAVTFLTSTTAVLAAVEPYGQCGGVNHQGETACTDGWTCTKYSDYYSQCIGGQNGGGSAPASTVETSATTAEPTTAPEPTGNVTSIVVVPSAAPVPSSNATTFETITSTPTPTSVDPEPETEATSSAVVVASSSTAAATSSSSSATPSTGGSGKNGADCSLDAAFKAHGKKYIGVATDQGRLAAGDNAQIIKDNFGQVTPENSMKWDATENVEGTFTLDGANALVAFATENNKLIRGHTTVWHSQLPTWVSSISDKTKLQEVMVAHIQKLVTTYAGKIYAWDVVNEILNEDGTFRSSVFYNVLGEDFVALAFNTARAADADAKLYINDYNLDSASYAKTKAMASKVKAWVAAGVPIDGIGSQSHLANNWPIADFPAALKSLCDVVSECAITELDIKGAAASDYETAVKACLDVENCVGVTVWGVADPDSWRASSTPLLFDASFQAKDAYNGICSILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKJ0</accession>
    <name>A0A9W9CKJ0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_007573</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKJ0"/>
    <dbReference type="OrthoDB" id="1431934at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20335">
        <property type="entry name" value="BRcat_RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20354">
        <property type="entry name" value="Rcat_RBR_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047548">
        <property type="entry name" value="Rcat_RBR_RNF14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="91"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="26"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="462"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="591"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="481"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4367043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="616" mass="67156" checksum="E6EC24026854BB2C" modified="2023-11-08" version="1">MAPSTRLRSKPERRVDYSLTKRLGARGNADLLEDPPEPDAFVRGQSEVPDPKALDTKAVSKDAIRIKGGKVSKLTAVQKEAAKAASKSRKNEKECAICAVVKSASYCFKVFNGSNICNHFDNICGVCIRKLLKTKVVARQLTEPALECPYPHCNHVLEVTELKKVLTRGAYDEYDDHLVKYLLWSDESYIACLSEACGIYFSKEACNDGANSKPKVACPYCAYEICLKCNRPWNNHDSGGCDEARKEEDKLSEEALKSLGAKPCPNCGVNIEKNGGCDHMICQRCRENFCWQCLAPYSPYMQHAEGCGSGRVNVAADMANWAPDNLTVPQVNNLIRQAQGRLDNPAEHPVPAPGDDELGPPWPAHVPLPLPERGPARHPAFEDLTPEEQNAELHATATHARAQAAMLRAQAIAARGTPNVDFQDMTPEAQIAEIHAQAAHGMARGAMRRAQAHDEATAAALGAIRQPPGGTNAPRRQLPPGLLPPPRQLPAYPGPIPPQRQLPADLDERLPAPNPAFRDMTREAQIAHVHAVTARARARAAMIRDQANTARANAAAHAAIRRPRGAIDTRQGQRIADNVPPQLLEIMSRMATPPSAYTHPDFEDRNGDDAPGDAPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKW0</accession>
    <name>A0A9W9CKW0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein HIR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">HIR1</name>
        <name evidence="15" type="ORF">N0V83_007144</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Required for replication-independent chromatin assembly and for the periodic repression of histone gene transcription during the cell cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the WD repeat HIR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKW0"/>
    <dbReference type="OrthoDB" id="1741719at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000417">
        <property type="term" value="C:HIR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000290">
        <property type="entry name" value="Protein HIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001557">
        <property type="entry name" value="Protein HIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055410">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031120">
        <property type="entry name" value="HIR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011494">
        <property type="entry name" value="HIRA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019015">
        <property type="entry name" value="HIRA_B_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13831">
        <property type="entry name" value="MEMBER OF THE HIR1 FAMILY OF WD-REPEAT PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13831:SF0">
        <property type="entry name" value="PROTEIN HIRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24105">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07569">
        <property type="entry name" value="Hira"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09453">
        <property type="entry name" value="HIRA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0678">Repressor</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11 12" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="CAF1B/HIR1 beta-propeller" evidence="14">
        <location>
            <begin position="29"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="66"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="126"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="168"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Protein HIRA-like C-terminal" evidence="13">
        <location>
            <begin position="729"/>
            <end position="967"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF24105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4367560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1043" mass="113184" checksum="BA052F4E6368B40F" modified="2023-11-08" version="1">MHLIKPTWLTHPGELKDFEVYSCHVSPDGSRLVTAAGDGYVRIWSTDAILNSNNPEYTKPKQLAAVSHHSGTIHAVRFSSNGRYLASGADDKIVCVYALDSNAPTHAAFGSNEPPPVENWRVIRRLIGHDNDVQDLGWSFDSSILVSVGLDSKVVVWSGHSFEKLKTLSNHQSHVKGITFDPANKYFATASDDRTIKVYRFNSPPPNATQQDQVNNFVLEHTITTPFQTSPLTTYFRRCSWSPDGAHIAAANATNGPVSSVAILARGTWDGDISLVGHEGPVEVTAFSPRLFYRDPPKPEHDGSVYQATVTIVACAGQDKCLSVWNTSYPRPFMISQELAMKSISDLAWAPNGETLYATSLDGSILTLVFEPGELGYPASLMENEKTLSKFGAGRRVGIVEGTDALLLEESSKSGELKGVQGRMGALMGDGGASLPPTITNGTNGILPTTNLTNGFSSTAPAPAPTEPPPDQRVEKLKQRVTVTKDGKKRIAPMLVSSSSGVGSSSLPQTQLISATTTSGGRSDNPHNVLDLSKPYDGFPKGGLASMLIGNKRKFAEIEGDEDRQLERRLAASVRPGGAAIVLNSENGLIPPAAAPPKSNELQEPPKVLRPAIVNPSLSVSQVRLAVPKVRSVIVRTMDGSEPPQNGVDANTGKAEIPDTVILEARNAAGPSRTGRPQDHDPTRITCTSKGQSLWQDFLPKAALLVTGNFNFFAAACEDGSVYAWTPAGRRILNAMVLEAQTVIMDCRGWWLTCISAVGMCYIWNLKTMSAPHPPVSLAPILDIAAYAQGPHLTRSPGIVFARLNSAGRLVVAMSNGDGYTYNPLMFVWNRISEPWWGVASQYWNSTDSSVGNIRSSQDKHTPTEKDDQVSIENISAGIIPALERNTTNQSLLQGRAFHLQRLIKALISAEGFEALESCASIAHLENRVVAARTLGAREEFKIYLTMYIKRLGAEGLKGKIEELLRSFSGDLIAEDEDDESEEFKEPEVICGWKREELLKEAVLILGKHRDLQRITVPYARLLGIVTEGQRPDAEQQAMITDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKY6</accession>
    <name>A0A9W9CKY6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_006142</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKY6"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000060">
        <property type="entry name" value="zinc finger protein 436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000176">
        <property type="entry name" value="zinc finger protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="309"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="337"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4369059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="371" mass="40258" checksum="6A79CF6FD578CCE3" modified="2023-11-08" version="1">MVHGSVRERDALQHAQHSPYYQAAQQSRGYPGVNVAPSPYSTQPQPQAQQAEYQYLRQRPQSPPSPPAEEQKPSLPSISHLLEFAGGEKAASETVAQSPQSQQQRLSSSPQLRQAQHRAAGAMQQPPQMQTSRPEQSAAYASVPAVGNPRMTLPPTPPMHPDVAHDGTQSPSAASNHSVASVPYFVGGSLNNMEPHHQRQSVSSMPSMKRDSLPSHTMSPYSASVYGQSPYASSPGGASATSFYSPEQHSYNSAAMYAQRPLPSNFQPQTMPLPVPVPSPSATGSNPWQHHHYISTSSQSQFPQSQDRYICSTCNKAFSRPSSLRIHSHSHTGEKPYKCPQSGCGKAFSVRSNMKRHERGCHAATSTTLTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-06-18" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMA6</accession>
    <name>A0A9W9CMA6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ATP-binding cassette transporter yor1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">YOR1</name>
        <name evidence="12" type="ORF">N0V83_005212</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18597">
        <property type="entry name" value="ABC_6TM_YOR1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18606">
        <property type="entry name" value="ABC_6TM_YOR1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002040">
        <property type="entry name" value="ABC multidrug transporter (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000010">
        <property type="entry name" value="Multidrug resistance-associated ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF464">
        <property type="entry name" value="ABC-TYPE TRANSPORTER CICA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="377"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="459"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="491"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="879"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="917"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="996"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1018"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1108"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="180"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="596"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="884"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="1195"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="558"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="817"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="573"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="586"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="822"/>
            <end position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4370691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1447" mass="160650" checksum="ED28236BA3F398DD" modified="2023-11-08" version="1">MTKSASTSSLDENEKEKTFGLVDPQAAQENPTEYEPIKTEKDLEAGDSQSTRRDLSRIQSSYSEYSDNDSDAKSSVRKRKWYKKLNVLKNNPPPVPKQRDVCPEYTASLPSRLTWQWMHPLMAVGYKRPLEKNDIWAVNPDRSADVLATRLEAAFKARRAAGGQRPLLGALFDTFKWEFIIGGLCQLSASLIQSVAPFVLRYLISFAVKAYVSQHTGAPAPNLGEGFGLVIAITVLQFFQSLATNHFMYRGMMIGGEARGVLIALIFNKAMKLSGRAKAGGQAIASAPPPDIKPGSVAEKKWYKRLLKSKAKKQAPAGAAGVAGDGEGWGNGRIVNLMSTDTYRVDQASGFFHMIWTAPVGILVTTALLLVNLTYSALPGLGLILIAMPLLGRAVKTLFRRRVAINKVTDQRVSLTQEILQGVRFVKYFGWETAFLERIQNIRKKEIHGIQILLTIRNAILAVGMSMPVFASMVSFITYSQVHSNLNPAPIFSSLALFNSMRIPLNFLPLVIGQVIDANASIKRIQEFLLAEEAEDSATWDYNAKEAIVMKDAEFTWERHPTQDPEGNPATAGDKKAEAMKKKEASTQPAQGNGNATPSDAVVEQETPFNIKGLNLTFGRNELVAIIGSVGSGKSSLLAALAGDMRRTNGEVVIGASRAFCPQYAWIQNATVRENIIFGKEFKKKWYDQVVDACALRPDLDMLPHNDATEIGERGITVSGGQKQRMNIARAIYFDADIILMDDPLSAVDAHVGRHIMDNAICGLLKDKCRILATHQLHVLSRCDRIVWVEEGQVQAVDTFDKLMAHNEGFQNLMSTTAKQEEQEEEKETVEDEVEADVKSTKKQRRQKKQAALMQVEERATKSVSWDVWIEYIKAGGGIWVGPLVFILLVLSQGANIVTSLWLSYWTSDKFGYSEGAYIGAYAAFGVSQAMFMFLFSFAVSVFGTEAGKTMLHRAITRVLRAPMSFFDTTPLGRITNRFSKDIDVMDNTITDAMRMYFLTLAMIISVFILIISYYYYYAIALGPLFLFFMFSAAFYRSSAREVKRHEAVLRSTVFARFGEAVMGTSTIRAYGLQDQFSRSVRAAVDDMNSAYYLTFANQRWLSVRLDIVGILLVFTTGVLVVTSRFSVNPSIAGLVLSYILTIVQMIQFTVRQLAEVENNMNSTERIHHYGTQLEEEAPLHMGEVRPTWPEHGEIVFDNVEMRYRAGLPLVLKGLSMHVRAGERIGVVGRTGAGKSSIMSSLFRLQELSGGSIVIDGVDIAQIGLHDLRSKLAIIPQDPTLFKGTIRSNLDPFHEHSDLQLWGALRQSDLVSNEATMDDHSGRIHLDSVVEEEGLNFSLGQRQLLALARALVRGSQIIVCDEATSSVDFETDAKIQKTIVEGFRGKTLLCIAHRLNTVITYDRICVMDSGLIAEIDTPLNLYEKPGSIFRSMCERSGIRRDDFHGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMA7</accession>
    <name>A0A9W9CMA7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">IKS1</name>
        <name evidence="12" type="ORF">N0V83_004541</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMA7"/>
    <dbReference type="OrthoDB" id="1405469at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000306">
        <property type="entry name" value="IKS protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000699">
        <property type="entry name" value="Probable serine/threonine-protein kinase iksA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF138">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE IKS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="168"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="38"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="502"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="526"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4371324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="588" mass="65780" checksum="2B819A39679D55DB" modified="2023-11-08" version="1">MDDRERLRSMSLIPYAPAESREIVLRHGSAVVVYDQRSKQLSLRDASQSTTVESTTCPYCRRPYREPSPNEEDESDEHEHSPGIPDADNGFVNPGYFQMLRRSQPGSEEGSRPASPHKQLAPPPLQGGPSEFHAPDGAEFVGSTPAPPARSQGISARAFSPNYFKTFFVEERELGRGGKGVVLLVQHVLDGVQLGKFACKRVPVGDDHEWLEKVLIEVQLLQTLSHQNLVSYRHVWLEDYQISNFGPSVPCAFILQQYCNGGDLHSYILDSAKSTVTKEQLKERLRRRSKGQLEDPEDLHGPRRMHFDEIISFFKDITSGLHHLHANGYIHRDLKPSNCLLHNTGQKIRVLVSDFGEVQSANIARTSTGATGTISYCAPEVLRQERPGGSFGNFTTKSDIFSLGMIVYFMCFGRLPYRNADGIDEDNEDLDQLRVEISSWAGIDDEQRVRSDLPDKLYRSLKRLLALNPDDRPGTEEILHVLKSPTLFDEFNAYAGPSGLDEFSPRISRADTPSPSSAQAAKKRTSISYQRTSRSRLSASVMERSPSPPPAQPMRRQSSGDGAVIWRPRKLEFRPPSPAVREERSGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMI5</accession>
    <name>A0A9W9CMI5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alkyl transferase</fullName>
            <ecNumber evidence="3">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RER2</name>
        <name evidence="5" type="ORF">N0V83_004783</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMI5"/>
    <dbReference type="OrthoDB" id="4173905at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF43">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT DHDDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="216"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4371564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="358" mass="40637" checksum="97F01D3C8789D06B" modified="2023-11-08" version="1">MGSLHDSRIHKWFATSPPVAWTVRQLRELLIGSLKQGPIPQHVAFVMDGNRRFARNHHIETVEGHNLGFESLARILEVCYKTGVKVVTIYAFSIENFKRSKHEVDALMSMAKVKLQQLAEHGALLDRYGASVRVLGQRELIQPDVLQAVDKAVAMTAHNKKAVLNVCFPYTSHHEITTAVMKTVETYSTPIHNLSTPSKRTFSESHITQTIRKQMLEEDTGSGAEQPESRSDSPSLKATGQAEDERSSSTSTAINSSDKDEYQPTFLDPETISAQTLNDNMMTADAPPLDLLVRTSGVERLSDFMLWQCHEDTSIVFLKCLWPEFDLWQFLPVLMEWKWQQKKLREEQQRSRGRSKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMK0</accession>
    <name>A0A9W9CMK0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_004988</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMK0"/>
    <dbReference type="OrthoDB" id="3696517at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4370469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="294" mass="32778" checksum="38558BDA1C39091C" modified="2023-11-08" version="1">MTPFPLLSMRKDGNDESTVEETVLSHEAEARTQSMGVDQVLLKLNRVGVLAKDDLIPTHTIIENCLVKRLAEVLPHDLLFVGRDECTFKAPLITATAYSVYLAEGLKGKVKMMVETKAWPNYAVGLVIDHIINHAMYRVKVPLKKHEILYATNQPKTPGPAIPAGTAIAHVHLYVALLAQDWGMIGLLMLAYNALVQALAKPDQPTLDQFIAMIKGTEVFTEKYCIHSRTHGSHWLRQILCALGTYGAINHEIWMEQDKAKYLEFFPSAPYFAPFLLLCQSQLPSTVSTDVRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CML1</accession>
    <name>A0A9W9CML1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Enolase</fullName>
            <ecNumber evidence="3">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ENO1</name>
        <name evidence="16" type="ORF">N0V83_005079</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(2R)-2-phosphoglycerate = phosphoenolpyruvate + H2O</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Mg(2+) is required for catalysis and for stabilizing the dimer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CML1"/>
    <dbReference type="OrthoDB" id="1739814at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.10:FF:000001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000002">
        <property type="entry name" value="Enolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 16" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="15">
        <location>
            <begin position="3"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="14">
        <location>
            <begin position="143"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="347"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="374"/>
            <end position="377"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4370558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="438" mass="47313" checksum="1F10C0ED6CA545DA" modified="2023-11-08" version="1">MTITKIHARSVYDSRGNPTVEVDIVTETGLHRAIVPSGASTGSHEACELRDGDKSKWGGKGVTKAVANVNDTIAPALIKEKLDVKDQSAVDAFLNKLDGTENKTNLGANAILGVSMAIAKAAAAEKGVPLYAHISDLAGTKKPYVLPVPFQNVLNGGSHAGGRLAFQEFMIVPCEAPTFSEAMRQGAEVYQKLKSLAKKIYGQSAGNVGDEGGVAPDIQTAEEALDLITKAIEEAGYTGQIKIAMDVASSEFYKTEEKKYDLDFKNPDSDKSKWLTYEQLAELYKSLAQKYPIVSIEDPFAEDDWEAWSYFFKTSDFQIVGDDLTVTNPEFIKKAIELKSCNALLLKVNQIGTITEAIQAAKDAFGAGWGVMVSHRSGETEDVTIADIVVGLRAGQIKTGAPARSERLAKLNQILRIEEELGDNAIYAGKNFRTAVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMU6</accession>
    <name>A0A9W9CMU6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cysteine proteinase 1, mitochondrial</fullName>
            <ecNumber evidence="8">3.4.22.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LAP3</name>
        <name evidence="10" type="ORF">N0V83_004812</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Has aminopeptidase activity, shortening substrate peptides sequentially by 1 amino acid. Has bleomycin hydrolase activity, which can protect the cell from the toxic effects of bleomycin. Has homocysteine-thiolactonase activity, protecting the cell against homocysteine toxicity.</text>
    </comment>
    <comment type="function">
        <text evidence="6">The normal physiological role of the enzyme is unknown, but it is not essential for the viability of yeast cells. Has aminopeptidase activity, shortening substrate peptides sequentially by 1 amino acid. Has bleomycin hydrolase activity, which can protect the cell from the toxic effects of bleomycin. Has homocysteine-thiolactonase activity, protecting the cell against homocysteine toxicity. Acts as a repressor in the GAL4 regulatory system, but this does not require either the peptidase or nucleic acid-binding activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.</text>
            <dbReference type="EC" id="3.4.22.40"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homohexamer. Binds to nucleic acids. Binds single-stranded DNA and RNA with higher affinity than double-stranded DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.40" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMU6"/>
    <dbReference type="OrthoDB" id="2666448at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070005">
        <property type="term" value="F:cysteine-type aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043418">
        <property type="term" value="P:homocysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00585">
        <property type="entry name" value="Peptidase_C1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000021">
        <property type="entry name" value="Bleomycin hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004134">
        <property type="entry name" value="Peptidase_C1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363:SF2">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03051">
        <property type="entry name" value="Peptidase_C1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005700">
        <property type="entry name" value="PepC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0788">Thiol protease</keyword>
    <feature type="active site" evidence="9">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="433"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR005700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005700-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4371593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="512" mass="57414" checksum="3C566CD301E82896" modified="2023-11-08" version="1">MGANQSRPSDAAINEKLLERLQALHMKDERPMNEKDGYVVVGGEAPPKYTPGVKYQRDVSAAAISEWEKELMEDPKNRLALAALSSNPANAVLSSRSATISDTQNFNIKIPFEGSPITNQASSGRCWLFASTNVFRIAIMKKYKLSEFQLSQAYLFYWDKIEKANYFLESILDTTSEEIDSRVVQALMASPVGDGGQWDMVANLVQKYGLVPQSLYPDSFNATNSSTMDRLITTKLREDAVRLRSIVSSAASPEVIQATIASEKEKMLREIHLILTLMLGPPPPSDKAFTWEYYDRDGNLCTVRTRPTAFAKELSDNRTVRALSGTDVHQLFSLVNDPRNPYNRLLSVKRLGNVWNGRPVTYVNVDMKTMKEACIAMLKRGMPIFFGSDVGKYSDSTKGIMDTDLYEYELGFNIKLGLSKAERLQTGESQMTHAMVLTAVHVVDGKPVRWRVENSWSDRVGDKGYFVMSDAWMDEFVYQAVVDPGVVSSTIRKVLEQKPKMLELWDPMGALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNE1</accession>
    <name>A0A9W9CNE1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004664</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNE1"/>
    <dbReference type="OrthoDB" id="2951834at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF7">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="333" mass="37375" checksum="D228436890784DAC" modified="2023-11-08" version="1">MDRKARGVLGKRGRKDKATEKKDVGKKETVAKRPKVQEVECNGAAAQAPQSSRVTRSKHGMLTPEPSKLTESQVAQVAQVASPEETTDTPPHTFPFMKLPAELRVHVYRMALEREVSLFLHAERALDKPEEESYSTASSSPTLNNQVPYFGTGIIRPEPRALTAVERTPLKDPILPAILRLNQFIYKEARQVLYSDNVFTLCLTSGIYTLSTLHQRSRSLIKHVVLTIPSHHDILDGFADLVRLGLRYCWGLKSFKIILQATLPDDGRGVTGATSVYANAFHILRWLPKGCQVILEGCVNESVMKVVKEEGRLMSVLDETSYLKRQHQMPERH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNP9</accession>
    <name>A0A9W9CNP9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Short chain alcohol dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_003674</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNP9"/>
    <dbReference type="OrthoDB" id="47007at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24321">
        <property type="entry name" value="DEHYDROGENASES, SHORT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24321:SF8">
        <property type="entry name" value="ESTRADIOL 17-BETA-DEHYDROGENASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4373379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="288" mass="31360" checksum="EFEBCB20184DD25A" modified="2023-11-08" version="1">MASAEIQTVHKNIIISGGARGIGRALTRMMLEAGHHAYIFDIDEEELEHTTKVHLKKYHEDGKLGSATCDLRDVEDIRNKVKKAAEFFNERIDVLINNGGIASPYWKDGKTMEDPDTLSEWRAYIDTNLTGPFAMSQACIPYMKARAHDVVKGAHFENADNSSPAGPCIIHIGSFRAHQSDPNQEGYASSKAGQLGLMHSMAISLGSLGIRVNLIAPGRIKVAHECKEGDEKGTDWAGQVSQKDVDDHPTNRAGRPADIANAALYLIEAGFITGQDITVDGGALRKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNR4</accession>
    <name>A0A9W9CNR4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_003537</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNR4"/>
    <dbReference type="OrthoDB" id="5141738at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000082">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF74">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="388"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="433"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4373243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="463" mass="51383" checksum="3C3002FB5190A0F2" modified="2023-11-08" version="1">MLNLAAGAVTPMSRHFNEYNEYKLILPTSMYLVGYVFGPIIWGPLSESNGRKGVMIYAFAMLTIFSIASAVAPNFAALVVFRLLVGIGGSCAISVVGGICADIYHDPVSRGRSMAVFMAATTFGPILGPPISGFIAVISWSWAFWIGAVFAGASWPLFLFFPETYGPMILKRRAKRLRKETGDERFVAPLELEKTDLNHIVTVILTRPLRMICFEPLVLFTCLYLSYAYAIFYIFLQSYPIIFTGIYHFNAGETGLSFLPIGVGAIISAAMYLTWDSIFARAQRADKAWTKNPEMHRLPLACIAGPFFVISSFWLGWTAKESIHWIVPTLAGILFGMGYLCLFMALLNYLVDAYAIFAASAMAAASLSRSSFGAILPFAAKPMYRAMGVAWATSLLGFFSVALCVIPFVFLRFGARMKERSKFCQYLKAKKAEEEERREAERREGREKGTLVEANEAKPQEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP75</accession>
    <name>A0A9W9CP75_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP75"/>
    <dbReference type="OrthoDB" id="3777052at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="514" mass="54425" checksum="748C1F4C60704FD4" modified="2023-11-08" version="1">MGDLTMDPSTDAVSSSSVSISVPASETLSAAASSTPAPSATLVSSSSSDSVQSSTPAASSSAASTAFSNSGASSISSASSTTATDASSILFSSAASSSASSDAVTTQPPSSTVPTPSSCTPTIIDGGFDNFSYPPDATSTLGYGTSDDWSYTRSFFETDGGWFANGGFGPSYSGTIATSVYTAYTNNTADIFYDIKYKHPIALCAGTSYDVSGWMRIHRRNSQYSEGCRVTAFLNGQQAFTDRLGYYDQNYRYVHGTITPSTSVTDAELYFRVYCRAPQAPTLSAPRGIKLDDVAVVPVPAWTLPAAAVLATRTTSVRTDTTPTSIFPFPSPCQPTDITVPRTGCPLYDEQLEDGGFEVCSYSHWSYLEAGTQNYDFDNPLDLQLTQIVSDAHSGQNSLRVVFNSTGGSIGIYPRQVQGCVERSYVYSYWAKQAGSGCTVQFYWRTVNTGTFTPPVDSWGQYSGRTDSNPTGTLLFGGVLEVRVTCAAGGLENAVWLDDVSFQQVPWNGQAVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ24</accession>
    <name>A0A9W9CQ24_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ24"/>
    <dbReference type="OrthoDB" id="3439209at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="482" mass="53526" checksum="6F11655BA80139FF" modified="2023-11-08" version="1">MAQGQLLHCQSSYNDLCDLRQDFSQPQPGLDYTNHFDPTFANPTAQAMLQRQYRPESGMPSSTWGFPDTMSREQMPPVATPSGSAPNNGFSSPIHFWEGGPTFGSNAVAWSNAPSMQPIGTSQPEYHYLRNSGLSYNDNAAQAPMVKAGDTYGGHKNWSAPRSNAVMRAADLDTNESHSPGSYLEGNLESPSSMLDQATPGDDWNRYSPTFPYVKESASGAASPELHKTEGFGEAEFAGLPQLGMSKAMPAGPYRQAGQWVGSYENTREARSVYSYPHGSGPDASPWYHSEFSLDAAGLPLMTRDARMDQARSDNSSNASFPQLAPQQIASRSDSRVGVPSRGRYQPRFQVPDAQALHRVHNDILLQGRADGETYKEILRRMPGKTPAESTLRGRYRSLIKARENRLRKPVWKERDIELLNEFVDLEFDRVEHAHGRALGVDQKLRKVQWKRVADYIVAQGGSYHFGNSTCKKKWVEINLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ39</accession>
    <name>A0A9W9CQ39_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">asparaginase</fullName>
            <ecNumber evidence="2">3.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_003053</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-asparagine + H2O = L-aspartate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21016"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="EC" id="3.5.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the asparaginase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.1" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ39"/>
    <dbReference type="OrthoDB" id="542841at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006530">
        <property type="term" value="P:L-asparagine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08964">
        <property type="entry name" value="L-asparaginase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1170:FF:000001">
        <property type="entry name" value="L-asparaginase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004550">
        <property type="entry name" value="AsnASE_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020827">
        <property type="entry name" value="Asparaginase/glutaminase_AS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500176">
        <property type="entry name" value="L_ASNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00144">
        <property type="entry name" value="ASN_GLN_ASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="L-asparaginase N-terminal" evidence="7">
        <location>
            <begin position="49"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase C-terminal" evidence="8">
        <location>
            <begin position="271"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="active site" description="O-isoaspartyl threonine intermediate" evidence="5">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001220-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4374312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="391" mass="41650" checksum="609975778F4CD986" modified="2023-11-08" version="1">MRFSILTTAALTVATGAAPTIPQPQSRQATTASERLGLTWLGNNSSLPKILILYTGGTIASGSIYGALDDTQYGQLQITGEQIIARNPYLLNNTQLAVSNWTIEGDSTGTNDALVMNMTRFAHDALCSEDSDIIGAVYTHGTNSLEETAFLMDSLINCGKPVVGTGSMRPWTHLSFDGDANFFDAVMLAASPGARNRGLMVAFNSRIIPGYWATKLHSTNPNAFGPTATGDLGLFLNNLPIFYNTASQPLFKHYFDISEVSAYSSYPALPKVDILYAAREFDGKLVLNAQANGAKGVVIAGTGNGGVPSGQDEITEALESGLQIVVGTRSPFGPSSPSITPTYAKSGFVHVIQTRIMLQLAIASGMNMNQTIELFEGGIRKAMGQSWYPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ96</accession>
    <name>A0A9W9CQ96_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_001873</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ96"/>
    <dbReference type="OrthoDB" id="2789670at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11065">
        <property type="entry name" value="CYP64-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050364">
        <property type="entry name" value="Cytochrome_P450_fung"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46300:SF4">
        <property type="entry name" value="CYTOCHROME P450 98A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46300">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4374797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="539" mass="61108" checksum="39C9322438ED5932" modified="2023-11-08" version="1">MAIESLLQLPLYVWLSLFLVVPIVTLLHDVWVWLRMPPGPTPLPFVGNKFGLPKSQPWIQFQKWSKIYGPIFTIWIGRKPTIVISDPNIAVELMEKRSTKYSSRPRMVAMGEILWDGASILVQPYGKEWSVRRKLLHQALTPKALRLYKPVQTAEASRLCCELLQSPENWEKLLERFTSSIVFCVAYGHRIDSLNAEVIHQRFKFMHYSASLNVPGKYMVESFPILKNVPDFLAPWKVEIKKHGREEAVENMALVDVVRADLARAKTRGDDIPDSLCKLLLELREKEHIPLSDRDFSYIPASLFGAGSDTTASTLCTAFLALITHPETLRAAQQELDDVVGSSRTPTFDDESRLPYIRALAKEVLRWRPVAVLGGTPHASTEDDRYEGYYIPAGTTVLGNSWAINLNEEYYPNPHHFDPLRFLDSALAERSKAPTPLTGKPHPAKAGHSSFGWGRRICPGADLAANSLFIALAKIIWAFNILPIEGVKYDIFAYTEGFNIRPQKFKCAIEVRSEAHRQLLESELMEAEAVLERFTPFRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQK6</accession>
    <name>A0A9W9CQK6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_001624</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQK6"/>
    <dbReference type="OrthoDB" id="443402at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056693">
        <property type="entry name" value="DUF7791"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF5">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25053">
        <property type="entry name" value="DUF7791"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="3">
        <location>
            <begin position="269"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7791" evidence="4">
        <location>
            <begin position="557"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="141"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4376341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="921" mass="105129" checksum="CFCD1AD6CA94F0EB" modified="2023-11-08" version="1">MPLEALAAVGLASNVVQLFDFLCKTYAELGEIRKSATGLSDVNVDFENVAVNLKRLTANLASVNAPAGSQADQQLQELAIKITAVADQLLGAIAEFKTHEPRGKWKSFVHALQQKLGNATKIDKTAGKLSRLQEQLNTCLLQMMRDENSHVRKEIQNLIDESKRMETNTAQELQHLKTDVLSQIQNLETKSLLYGSRVEDLLKNVERLQSVNGRDVAKLSGRMQKLSHTLSKIQDESKSIYQDHRILRNLHFKTIKMREFRIPEAHTKTFEWIYSDSNEDSSFLQWLQHDHGVFWTMGKAGSGKSTLMKFEANHRLTRKHLKRWAAEKKLVIASYYFWNAGNDLQKSQEGLLRSLLFTILQNCPHMISELFSEFYPDPGYFEGDAAIWNPQSLLQLFTRLSQQAEPTTRFCFFVDGLDEYEGESIELIESLRALARSTMVKLCVSSRPWHVFKDEYERPNSCMLKMEDLTRDDIALYVQDMLEGHERFGTLQQRDSRYQELVQQIIDKAEGVFLWVFLVVRSLLRGLTSSDRISDLQRRIALLPPSLEDFFRHMLDSIEEVYRKDTFEAFQYVLHAPPSGPIPLIVFSYLDEEDPNFAFQISAATLSHKEISMREDDMRRRLDGRTKGLLEASPRRDASDIPTVDFLHRTARDFMVTRDMRHMLEKHLDPKFDADEALARAFLAQIKLGPLPRDGSLAVDPLSTVLYYVHESESNSYRPASKIVDELAVAVRNVYWDATIRRDGSFLGHLVANNILIYVAQWLATVPNLATQSKSPLLSVALRNVPRAAIASTQRYLNADMVELLLSKGAKPNQNLSFSTTIWAEFVAELHNYPRKYGKDGPTMLRTLELLISHSADLDIPVKIKELSNNSRLDSGNYLTASEIIRLRFPDDAEWLLSKAPQGASFGTAFLAWPFFRKSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRE3</accession>
    <name>A0A9W9CRE3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Alpha-galactosidase</fullName>
            <ecNumber evidence="3 7">3.2.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Melibiase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_001611</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.</text>
            <dbReference type="EC" id="3.2.1.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.22" evidence="3 7"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRE3"/>
    <dbReference type="OrthoDB" id="5795902at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004557">
        <property type="term" value="F:alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04081">
        <property type="entry name" value="CBM35_galactosidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14792">
        <property type="entry name" value="GH27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000197">
        <property type="entry name" value="Alpha-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002241">
        <property type="entry name" value="Glyco_hydro_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041233">
        <property type="entry name" value="Melibiase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452:SF75">
        <property type="entry name" value="ALPHA-GALACTOSIDASE MEL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452">
        <property type="entry name" value="ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16499">
        <property type="entry name" value="Melibiase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17801">
        <property type="entry name" value="Melibiase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00740">
        <property type="entry name" value="GLHYDRLASE27"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Alpha galactosidase C-terminal" evidence="8">
        <location>
            <begin position="292"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4376328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="517" mass="56271" checksum="2977674281D34DF0" modified="2023-11-08" version="1">MGWNSYNTWSCSPSEDKIKQSAQGLIDLGLDKVGYNFVTVDCGWPSRDRDAEGKIQWNATLFPSGGKALGDFLHGLGLDFGLYSGAGYLQCGSTDLPASLGYEQLDAESFAEWGGDSLKYDNCYATSNTTMVDSNSTESQSPARFQHMAAELDAVDRDIQYFICQWGIGTDVGEWAADIGNTWRISNDIYNAWRSIWRITNQVVPYFRHTTVGAFADMDMLIVGLNALSAEEERFHFGMWAINKSPLIIGAALDSDRLSQTSLDILSNTDVVAINQDPLAEQARLVRRYTEEEWDIWLGNLSGSRQILGIANWRNDSKSVGFDLSSLAIASANARDVWATKDIGTVSGTQNITLKGHELRLWLLSDIATSSPLASIAYYVAANASVSGSAQVVTCASGACLPIGVKIGKIGSNSSVTFGNVTASSTGKQLLGIDFINYDYAFTTAWDWGDNTRNMTITVNGLEAKRWAFPLSGGDWEESGRLVIEADGFVEGADNVVVFGSYENGWAPDLVGFEVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRX1</accession>
    <name>A0A9W9CRX1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRX1"/>
    <dbReference type="OrthoDB" id="3801238at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="485"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="504"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="514"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="561"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="619" mass="67681" checksum="6B155806B70360BF" modified="2023-11-08" version="1">MSSTSAMSSITPPSYVDDGVSFAKRIPHRLDSKHISRWIDSTDSSTSAYTGSFIDDGADMLPPAHDTPGFGPINKRFYKPRKKKEDKDDVWNEDMQKAISGDMARNLAKIKDLGKMEVVKRRRGDRANEKKKPVTMFLPPKWGPFVIREENGRVTVIDEDGEFDSGPLEDAEKERTEGMRWIRVTSSTEPTSVTSSTEVSTLLSSSLSTQSIYSHDAKAPRPREKDKKHSRRSQSHKRKKAHHHRHPPTPLTTMLESDYEDGYQLSGGEDNVMSPTQFFMTGGASGWPSSRTSTSVAAPAAKATTPYGSSNHDSPVKSRSPVCSPPGGWPSSPLPSPVKSVVVSEQSWGGGNDNRNAWGEDDSQPKERSERSSSSYRSERSNTSRTSRRGQGFENTSTRTYSVYRAPTVQDASETSSEGMMRRYVQTGWGGSLKSGSGKQAVRNRKDQNTDYSAGSSHAGSEQDLGGNDERSGTGWDGYERVKTVSEVSVVGTGSLRSSRESHSQAPSRQTATACSHSSRPASHAHSDLGSQMGWGGGSRASRQSWSSEKARVDDDGWGGSQQKSSEGGRANSSSKRGSEKASRYANAYDEDNETYLNENWGGTPVRVGSRQTRVAGWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRY4</accession>
    <name>A0A9W9CRY4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Post-SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_000383</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRY4"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053201">
        <property type="entry name" value="Flavunoidine_N-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12350">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12350:SF19">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Post-SET" evidence="4">
        <location>
            <begin position="160"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4377558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="207" mass="22661" checksum="710F231A7DF37AF9" modified="2023-11-08" version="1">MSPAVITSTPPPSSSGKISSIKSSTKPVITTSSTILPPTPSTQIPAWVQPDLTRLLRVEHRPGSFASRSVSLVNLPAGAIFARITNPTPATVAYSSVQASRDLHIELNCDLVYINHSCQPTLVFDMQRWEVRVNPELESGLKEGDELSFFYPSTEWDMAQPFECLCGSKECRGTISGAKDMPAEVLKKYWLSSHIQELLEEKAANGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXC3</accession>
    <name>A0A9W8XXC3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_011029</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXC3"/>
    <dbReference type="OrthoDB" id="5979581at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="334"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="223"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="365" mass="41123" checksum="7D537192DC05C4C0" modified="2023-11-08" version="1">MPMENILVSGFERKPNENDTIQNNNITAKLGDLGIVMEPMKGLVQPIAYRAPEVYFRREIGPAIDIWSWGIIYCQLLEAQASFHKTGLYDELLQGSYLHKEESMQKAIVNDFGLNSIDYYENYTSRYRDHRHREGQHWDHLIKKGLPEKEVEFLRWVLNPVPSERPTAQQILDSGWLTPDKDEGSGILNALARRVMNVRKRSEPSLWTRYYDTSAKKAREEPVQQQAPVQHQAPVQHQSQPPVQAPAPYQASTQHAPPAQAPPQYQDQAQGHAQTQPPAEATPSVLPPYQYQYQPPGQAQAQIQATAQPPAPKPHAESQPHMFDTQTAVLAREERHPARPATPRASTTGRPSGGGTFLNFGAVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY35</accession>
    <name>A0A9W8XY35_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">4-hydroxythreonine-4-phosphate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_010678</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY35"/>
    <dbReference type="OrthoDB" id="74991at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005255">
        <property type="entry name" value="PdxA_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30004">
        <property type="entry name" value="4-HYDROXYTHREONINE-4-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30004:SF3">
        <property type="entry name" value="4-HYDROXYTHREONINE-4-PHOSPHATE DEHYDROGENASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04166">
        <property type="entry name" value="PdxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4362584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="22415" checksum="485BED469F18C9BD" modified="2023-11-08" version="1">MRSHTRRPRVAVTLGDAAGIGPELIAKLVSNSENLRKADIFILADKSEVSQAIADAGHVNVPITQHAGPEGVCILDDGTAPADQGQRAEVSEASGARCIYQLRRALQMHEAGEIDAIVFGPLNKSSLKKAGMTQEDELRWFADQLDFKGTTSEINIAGSLWTGRVTSHISIGEVAEKVTKERTLKAIELVNQLRFVKQTRCPPSTAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY73</accession>
    <name>A0A9W8XY73_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010942</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY73"/>
    <dbReference type="OrthoDB" id="5555533at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070096">
        <property type="term" value="P:mitochondrial outer membrane translocase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037652">
        <property type="entry name" value="Mim2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28230">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28230:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19117">
        <property type="entry name" value="Mim2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="111" mass="12537" checksum="0FA3240ED854C6D9" modified="2023-11-08" version="1">MSMSNSESFIEPPMSSSEADEIDSLPSSSTTSSVDDDEYVSDAEREWRESLQQLELLLTMVMVPYLGKYFGRKCAYWGWAKYMEWKYPVEIVISSPKTFKLAGAIEAAATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYM7</accession>
    <name>A0A9W8XYM7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_010700</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYM7"/>
    <dbReference type="OrthoDB" id="72851at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06423">
        <property type="entry name" value="CESA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050321">
        <property type="entry name" value="Glycosyltr_2/OpgH_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43867">
        <property type="entry name" value="CELLULOSE SYNTHASE CATALYTIC SUBUNIT A [UDP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43867:SF2">
        <property type="entry name" value="CELLULOSE SYNTHASE CATALYTIC SUBUNIT A [UDP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13632">
        <property type="entry name" value="Glyco_trans_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="8">
        <location>
            <begin position="159"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4361760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="379" mass="43388" checksum="2A41DE2F2CF2E2C9" modified="2023-11-08" version="1">MVCSEKMIEVLWYVYMLTNTYVSMVTVAEAVLSISVTREARNRVNKVAGNDFKPGAQPWPTPDDRLPILDLIIVAYLPNERDIIMDRVNYLCTKVVYPVDRIRINCVYNTPIAIEPLETELVDLQEKYSQLRVIKVPGSKSKADNLNYFFTLNTGADVIAIFDADHYPHPHNPRWCAERFMADESIGIVQGRCVIYNSRDSWLTRLIAIEFDKIYAVSHPGRAIMMDFGLFCGKKTTKEFNYLVITWVAGSNGYWRAELLKQHRMHGHMLTEDIDSALRAVKKNVKAVHEINAVSYELAPSEFQGFWKQRLRWAQGWAQASLVHMPMVWDQPEQGTRGLAKRFGVFSLLAVRETSYYLITQVGPQIASMLLARVETKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYQ7</accession>
    <name>A0A9W8XYQ7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_010514</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYQ7"/>
    <dbReference type="OrthoDB" id="4898680at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF82">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="4">
        <location>
            <begin position="295"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4362421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="677" mass="75657" checksum="4F53A9524446DE03" modified="2023-11-08" version="1">MATPSVIVADSEVLRSAVSTIRHPSPGLEMPLIRPSKATLNSDPMYPPPSPAQGDKLADAVAADVYHPGFLGPGSYAVLLPQDDESERPRERELSVASERSDRELTHQHVLTKSMRYQMASDVLSTLRHYNSIRELVLWYNASNEAGVIPARLQIDAINAMESVVDKHNLRRSPPSSQLIAQVLESTSRPFTISQSLEPHDFHILCSGENLRFEIIGFLLATAGRSLTFGFAPDLFSDPANRGLKSRFIDELLRASTTCLFLCTMLATVNDVNIWMFYENYLFTTMMCGYAGPPSWRRIGELSTQMYTLGIHKESTSAGVPMWLRETRRRIFCSSYNQDKSISTFLGRPIRISKRHSDINLPLDLADDETTGDNVVLELAIQSLDSEGWNTKGRWLRASWIRLRHVSLRFREEILEFSFVKMDEEVERQLLDISQRIRTSWDSLPKHMRYWKTCWDEDVPSTVCLMLIIVHLTHWYNEFMIQKLLDHQSLTLNTALLRVSVDLLANVLTLGTIRDRSYDIHRDLLHAILLFGVPAASVLATALREQQQTGQQFPADISRAEIIRMLSVLISHLDAAAHVENSGARQGEANYNLCRKASKIFTRVIDAVLDAKALGSSEVTPSSEQLGLDLGLDMFTGPGLDGFEGMEFGGFGMQMGGAGAGGGNDGIDWGAIGQWSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYX7</accession>
    <name>A0A9W8XYX7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYX7"/>
    <dbReference type="OrthoDB" id="3792523at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="139" mass="16263" checksum="05D2026FB5E313BC" modified="2023-11-08" version="1">MDELLDLDVARYAKRLTFSPKFYHKTFWKEYLRFIDEETEEMTYSDLIAPELRDNYEEDNSDHLPPPEKGHPTRDSIGRHLVAKAQSLWAAKAAEQEAKRGENCAALARLFDSMSNLECVEITEWSCTLKEHHIIDYPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZH5</accession>
    <name>A0A9W8XZH5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZH5"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF147">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4363908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="36224" checksum="77C9AA180854B342" modified="2023-11-08" version="1">MDRHRFKQLILIIGPIVRIAPNTLSFNTASALSTIYGPRGANVKKGEWYRTFDIAAGTYSSFTETDREKHAIKRRWMSPAFSVDSMKANEPLIIDIVERFCETLKPADSGWGAKWNMSEMCTYLGFDIMGALVFGCDFKTVQEKENRPLANSVLPASGKSVSDNNRLIDYASSQVETQLASGDSEKHTTEHRIDFLSRLIGVEDKKTGWNPTFADLSTESLNMINAGADPFSSVLAGAIFYLVHNPEALEKATAEVRGKNLAYLQLRITLARVLWRFDVRDAVDERGGRGKKGLGVGGERGEFQMWDALGFGRDGPMVEVRVARGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZY9</accession>
    <name>A0A9W8XZY9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome-releasing factor 2, mitochondrial</fullName>
            <shortName evidence="6">RRF2mt</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Elongation factor G 2, mitochondrial</fullName>
            <shortName evidence="6">EF-G2mt</shortName>
            <shortName evidence="6">mEF-G 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">MEF2</name>
        <name evidence="9" type="ORF">N0V83_009807</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="6">Mitochondrial GTPase that mediates the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Not involved in the GTP-dependent ribosomal translocation step during translation elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZY9"/>
    <dbReference type="OrthoDB" id="198619at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01886">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03713">
        <property type="entry name" value="EFG_mtEFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04092">
        <property type="entry name" value="mtEFG2_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000106">
        <property type="entry name" value="Ribosome-releasing factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000007">
        <property type="entry name" value="Ribosome-releasing factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000514">
        <property type="entry name" value="Ribosome-releasing factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03059">
        <property type="entry name" value="mEF_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053905">
        <property type="entry name" value="EF-G-like_DII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030851">
        <property type="entry name" value="EFG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43261:SF1">
        <property type="entry name" value="RIBOSOME-RELEASING FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43261">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22042">
        <property type="entry name" value="EF-G_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="8">
        <location>
            <begin position="39"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="787"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="788"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="48"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="139"/>
            <end position="143"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="193"/>
            <end position="196"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4363512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="889" mass="96309" checksum="B8EDBDF621701155" modified="2023-11-08" version="1">MGMGVGWVWCARCVVRHNVKAPRRLLSTQTQLQADSLSRATRNIGIIAHIDAGKTTTTERMLYYSGHTRRIGDVDEGSTVTDFLPAERARGITIQSAAITFHWPPSPPNAQTQPQSPQLPPTFDTTLRSSISHNINLIDTPGHADFTFEVLRSLRVLDGAVCILDGVAGVEAQTEKVWTQSGHYHIPRIIFVNKLDRAGAAFSRTVKEIGTRLHGWPAVCQIPWWQGNNGDFVGVGDIVNLRGMLWQVGGDGRDIQAFSLEELGESDQKFAEEIKKARIALVEILSEHDDAMVEHFLDHDEDHLAISGTQIVESLRRVVLRAPQVIIPVFAGASFRNIGVQPLLDAVVDLLPSPFERPDPEISISNQSSTLSALLSSAAVAKPGKLARKQQQTQDQGLAIVEAKNLLACALAFKVVNDPKRGVLVYVRVYSGSIDKGATLYNTNLAVSERAPRLLKMYANDAVEVDSITAGQIGVITGLKHARTGDTLIVYRGLQMKGTPSGGLNTLQLRPINVPPPVFFTSIEPHSLSEQKHVQESLGILLREDPSLHLSVDEESGQTHLAGMGDLHLEIARDRLLIDFKAKARIGRIEIGYREMISTSTAPYTYDLDKPIAGKPTKASITASVEPIDVSIIVPGQEEIQVEDGAFETTYNLPDNNTLYILHPNLSRHDSASHKNYIPPHLSLPGILHSIQAGVSAALARGPFNGFPVANTRVTIDLDAQAHLLPETTPTAISMAARAAVGSSLRSVNDAAPSALLEPVMMVTIFVNEGSLGAVVQDISSARGGQVLSLDGSDSPVSSTSDDDLPRIDPNLIYTPPDPFASGSGDMGSGLANSQRQIVARVPLKEMVGYLNHLRALTGGRGTFVMTVDGFEKMGSQRQKEVLDAMREF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0I5</accession>
    <name>A0A9W8Y0I5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyl transferase CAP10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_010277</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0I5"/>
    <dbReference type="OrthoDB" id="541052at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF35">
        <property type="entry name" value="PROTEIN O-GLUCOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05686">
        <property type="entry name" value="Glyco_transf_90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="264"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="334"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="404"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="5">
        <location>
            <begin position="675"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="588"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4363157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1005" mass="113557" checksum="03612E146F389E1F" modified="2023-11-08" version="1">MDRRALIFPCGLVLAASYLSTATPVDYSFAFDKPLHTAGVVLLLTGLAVVAIEAWPCRSPQPQPPTRYIAIPLSHGQDRQSGEEIWTEAGQPGRRRAWSVRAVGALLAVLLFAICGRIGIFYRVIKDVECSGPSAMPFLPLVLALYHSIRHPSQRQYPAWSADSRPRTLLDRVSHFIFDGSTRYIIPSILLSISSFLVIVKSSSLRSTYICPVANSTATTIPSLQFFAFLLDSIIVQITYRLVDDGISMADDWTIHLQDGTSNHLLVGLTLIASSLVLLVAGIIVYPAMPEHREWMLSFPSEYLLALLRLSLMIPFMTLCFLKSARSYGVMSSVLMVAFSSAFIGVLRALGTGVSFSFPPKSTVGLVLCLTLLTMALILHLITDTNIETRIRSTVPVRLGRNQSAAFIVLLIAFSIGVVVYRRQGPVLEHPIATLIDLATVQHDQWASQAHRSKSLAEAVVHYRQRYRRDPPPNFDQWYHFAMTRSSIVIDDYDNIEEDLAPFSSFNPDDLRLRTATVLATNEGVGGIRIREGKAEVFSTVLDTQRWMMDGAASMIQQFAEFLPDMDLALNLGDEPRVAVPYERLQDALDNPQPYPTPEPSRPAKEFSTDRAKKWPDLEEVRVDPQFFEDARLKSSFQTYGSIACATDSRARKERHWNMKTFCHTCTQPHSMGAFVANWTLSADPCHQPDIANLHGMHLSPSTLMGTHDIVPIFSQSRAPGYADIRYPSPWNYMDKTKYEFDEKFPDPHFHDKEDVLFWRGATTEGLTTTGAWKGMLRQRLVHLLNNETARQPILLLKGDHSGQLEYVLKRTGDIKHYLETKTDARFVDEIVHCAGQDCVDQTREFGLAAPIEFKQHWRYRYLLDADGAGFSGRFIPFLKSNSVVFKSRLFHEWYEGRLTAWKHFVPVDLRLHDLFSSLAYFGGYGVEARNKRMMEGKTKQAEAIARQGKVWTEKVLRKEDMEVYMFRLLLEWGRLTDDARTEVGFRIGKGESRSRGVEREVVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0M9</accession>
    <name>A0A9W8Y0M9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutathione S-transferase 5</fullName>
            <ecNumber evidence="4">2.5.1.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gst5_2</name>
        <name evidence="4" type="ORF">N0V83_009455</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0M9"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03056">
        <property type="entry name" value="GST_N_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF2">
        <property type="entry name" value="HYPOTHETICAL GLUTATHIONE S-TRANSFERASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="92"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="217" mass="24658" checksum="083A2F2236F05195" modified="2023-11-08" version="1">MGLISNMHLYDSTIPSGNAYKVRLLLSHLKIPYTTTNLNILSVPPETRSVEFLAMNPNGRIPTLVLNDGTVLAESNAILFYLAEGTDYLPDDKLQKAQALQWLFFEQYSHEPYVAVWKFRTYWAPGGFDDLCESEVKKLRERGQEAIDVMETHLGSGREWFVGDHYSIADIALYAYTSAAEMIGFKIDGSVKAWLKRVEQQEEWIKIKNDPTGKCLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0U7</accession>
    <name>A0A9W8Y0U7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Choline monooxygenase, chloroplastic</fullName>
            <ecNumber evidence="5">1.14.15.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_009794</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the first step of the osmoprotectant glycine betaine synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>choline + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = betaine aldehyde hydrate + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:17769"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15870"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="EC" id="1.14.15.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (monooxygenase route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the choline monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.15.7" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0U7"/>
    <dbReference type="OrthoDB" id="426882at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019133">
        <property type="term" value="F:choline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00680">
        <property type="entry name" value="RHO_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03469">
        <property type="entry name" value="Rieske_RO_Alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.380.10">
        <property type="entry name" value="Naphthalene 1,2-dioxygenase Alpha Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015879">
        <property type="entry name" value="Ring_hydroxy_dOase_asu_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001663">
        <property type="entry name" value="Rng_hydr_dOase-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43756">
        <property type="entry name" value="CHOLINE MONOOXYGENASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43756:SF5">
        <property type="entry name" value="CHOLINE MONOOXYGENASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00848">
        <property type="entry name" value="Ring_hydroxyl_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00090">
        <property type="entry name" value="RNGDIOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="13">
        <location>
            <begin position="51"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4363499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="416" mass="48115" checksum="014E9059BB242B06" modified="2023-11-08" version="1">MSSLFHYFTFGNPAMEDAPSKESPTRALPASWYTSQAMYEFERRAIFSRRWLFITHSSRLKETGDWLRFKNVAGFDFIITRDRTGNINAFHNVCRHRAYPVVEEEDAGNKKILACRYHGWSYGLNGKLAKAPGYQELEFEKDQNGLFRIHTKVDGNGFVWVNMDASEIPEVSWEEHFANVDVQARYSHLNFDDYELDHTYELDCNYNWKIASDNFNECYHCPTTHPDIPAFLNLDSFDSDTVKGHIQHHCTSTPEQLAKGLDVHSTYYFPNVSMSASPHFMMIQKFLPAGPNHSNITYEVYKNKKSSSEDFHVIADMYERVMREDKVLCDAAQKNLNRGVFVNGQLHPKYEKAPLFFQKTVREVVIEHFQREKKEGREVWPARQKVEDNVGVSNEDEDICAGIACGNGAQKEVLAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0Z3</accession>
    <name>A0A9W8Y0Z3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008694</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0Z3"/>
    <dbReference type="OrthoDB" id="424402at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="314" mass="34654" checksum="1B1B99A2CA2F7094" modified="2023-11-08" version="1">MIKNQAIASLDPSKLSVMAKPKRMSDIFASSAEKVGDITITTATPPYSSEGPTLPFIESNTKPSAGSTKTGKKRKKNSHDSSTAPKKPTQPESLFTIDPALYQPWSLLPVEILKLLESQARLRATENVVPVVFTRNQNVKSGINRLKMYLGAYRNPNSSIEIPDALKQEDVVIAVSAQGQGTTKLVSIVDMVRRIVAPSAKDVGADEKVETWWMYTTLASVETERKLNKPTEEASHEAVHKSGETQDSEEEAFEPMDIDGPKDQGYENLDGADRIRIRTVPVLTVWMTRKKILAFKQAFGEQSLAVRLVQQGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y154</accession>
    <name>A0A9W8Y154_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF1772-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_010289</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the anthrone oxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y154"/>
    <dbReference type="OrthoDB" id="3750842at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013901">
        <property type="entry name" value="Anthrone_oxy"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35042">
        <property type="entry name" value="ANTHRONE OXYGENASE ENCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35042:SF1">
        <property type="entry name" value="DUF1772-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08592">
        <property type="entry name" value="Anthrone_oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4363169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="18936" checksum="98AC5297D756C74E" modified="2023-11-08" version="1">MSDNLIVKSVAGTCITFSFILAGNAITQSYMTVPALLVDFPPPGSPDHSARAKLLGRQWPLCWTVGNQFFRPISTLGFLGYAYAGYSVYREGVLARSDWKLFGVAAVMHLTTILHSALNMQPLNDKLEALASRAGEKELGEAEGIARKWASWNQLRLVTPVVAGGLALWNLLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y174</accession>
    <name>A0A9W8Y174_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010319</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y174"/>
    <dbReference type="OrthoDB" id="408743at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050411">
        <property type="entry name" value="AlphaKG_dependent_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696">
        <property type="entry name" value="GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696:SF21">
        <property type="entry name" value="TAUD_TFDA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="2">
        <location>
            <begin position="70"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="401" mass="44163" checksum="BFFAB222C94590E3" modified="2023-11-08" version="1">MTASLSSHFTQALKLDGDASTPLAFESFDLPSSNQRILGAPHPKGTVIPLALALRPSVHDVAPPSLDAAISTIKSLQAQGSLLSKKLATHGTLLFRGLPIKSADDFSRFAHAFGFSPHEIIGIVVDRPLLAPNVAPANEAPKEVLIYNHNESPQVPHAPEYIFFYSHRAPKEGGKTPISSSLELFHRAKEELPEFIDELAEKGILSKVTYKVDKQYTGGSTLKQAFGKDIEEGDDEATRRAKVEEQIKRYCRGEHTTWEWTDEGKTLVLTHRLPAIRTQPGSDLPTLFTGLAGYYKTFLSPDAPSVARRNITQQLFGDGTPIPEHYLKQLAEITDEIRVLHKWQEGDVLVYDNVIAQHGRQPWKGAQEDRVVLASLWDGVVPGKFGQSKGDWAQVVQASQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1R6</accession>
    <name>A0A9W8Y1R6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_008792</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1R6"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051938">
        <property type="entry name" value="Apopto_cytoskel_mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145:SF3">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="4">
        <location>
            <begin position="17"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="79"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="150"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4365174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="266" mass="29722" checksum="BA8ED7ECC6364C3E" modified="2023-11-08" version="1">MSLFHATATSLSDSSPTHYETLQLTSTASAADVKRQFYTLSKKHHPDRNPDDPTASTRFVAISEAYHVLSVPEKRAQYDAQLQHSQPQSRWGRNASDYPQGSYSSASFGARPASGLNKKRSTFRGPPPSFFKAGGYGRHGAKRAEYAYQGAQTEGQEGSKEAGQESYGDFGGFGPGQQSQGHQVPHFDDRRHKEMHENVYSHIHARRQRAQRMARVEVEYDRAGMVTNFLLVASIIGLIGVIVKVFGDRHEDRYDDKNTGKRKEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y204</accession>
    <name>A0A9W8Y204_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_009379</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y204"/>
    <dbReference type="OrthoDB" id="2341546at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001216">
        <property type="term" value="F:DNA-binding transcription activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.10:FF:000003">
        <property type="entry name" value="C6 transcription factor (Leu3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF21">
        <property type="entry name" value="REGULATORY PROTEIN LEU3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="10">
        <location>
            <begin position="64"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="600"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="658"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="103"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="601"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="661"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4363926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="742" mass="83736" checksum="4DE23ACDF871C129" modified="2023-11-08" version="1">MNALSLSPTTPLAQGISHHPFASPTSAHTRGFKRSASTDDENENGDGDTRPSSSSRRNTAVKRACNECRQQKLKCNVQQDPFVSCARCQKQGLRCVIEPNFKRVGKRNRNAEMEKEMEYLRERLAMYEGQPLSAQPTLNAPQRASDNQNFTPGPVPKLEDDAFLQTQHTQVAATSLLDLRSGSPMFYTLGAGEVRLGHSEVTELFTEYFQLYHPFLPFLDQVRSPDEYYAQDHRLLFWAVIAVAARRNGPRPSLLKDLAKPLSDLIWDSIRNQPNHHVVKALCLLCTWPLPAERTVTDPTFILCGAMMQVAMQIGLHQPTHPQDFQRAKVRLRQEDIHDRLRTWAVCNIVAQTVSTGNGQPSITLYDSTLDFKVDDEEHMRIIPPTLFVRLRQEIAASRINKLLYSANSHRFAEGAATSYMSLEADRLKEERVKEDRGSLDGVDNDLEDLYHYAVNLHLHLYSFFSPESRLERRDDLVALYFAATAYLERVFKLQRDGKLPHVPYYIMQMALAAGFALLKLLNSDFASKLPAERGRQFVLQTVDALRKAKVWPNDLLDRFAEVLAQLWKESSRGRSLHSLSQSPSISNSGMATMFNHHNVQQQNQQQRQPGQRRDSTGMMDDPLGLIIRSRMSMSVVFDCAWRWREAQVSGAEQLDNIVMTNPTNPDSSSSSTPPPGVVENPAHGLPNFNPHLNTLSMPLQLPNGLASANSYEFFDSVSWMLEAQTDWNSYGTGGFGNDFGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2L1</accession>
    <name>A0A9W8Y2L1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_008716</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2L1"/>
    <dbReference type="OrthoDB" id="4139357at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000171">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF5">
        <property type="entry name" value="MAJOR FACILITATOR-TYPE TRANSPORTER HXNZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="189"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="290"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="444"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="474"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="561"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="124"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="33"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4365098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="588" mass="64293" checksum="4C5B6967820332FC" modified="2023-11-08" version="1">MPRTMFINPFKSHDVSEFPDVLIPLDQATHRRSISSHRRVSLATSDRRGEDTTTKENKPENSPREPDNDASSGVVNHGMTLESLKAEIEADVAASDTDTPYDRKAKVINRALQDMGMGKYQWELFALCGGGWMADNLWLQGVALTLPQLSAEFGISETEIRYTTLALFLGLCLGASFWGTGSDIIGRRLAFNFTLFLAGVFGLASGGGPNWIGTCALYACIGLGVGGNLPVDGALFLEFLPQTSGNLLTLLSVFWPIGNLIASLLAWAYIPNFSCDSSTPVGQCKKEDNMGWRYLILTLGAITFAMFICRFFLFHLYESPKFLLSRGRQAEAVASVYGIAHYNKSHTWLTEDILNQIGGDAEVTGEDAKLSMFEVVKRSLGRFSMKRFRALFQDRQIGLTTALLWFQWTTIGMAYPLFNAFLPQYLANSGGGVENDTSTVYRNYAISAIVGVPGSLIACYTVELKYVGRKGTMAVATVITGVFVFLFTISSDSDFQLAFTCLEAFFQNVMYGVLYAYTPEVFPAPIRGTGTGMSSFLNRIAGLCAPIVAIQAGNANPKSPIYASGGLFIAAFFSMLILPIETRGKQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2L9</accession>
    <name>A0A9W8Y2L9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein disulfide-isomerase</fullName>
            <ecNumber evidence="5">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_008731</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Participates in the folding of proteins containing disulfide bonds, may be involved in glycosylation, prolyl hydroxylation and triglyceride transfer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2L9"/>
    <dbReference type="OrthoDB" id="427280at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02981">
        <property type="entry name" value="PDI_b_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929:SF132">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040901966" description="Protein disulfide-isomerase" evidence="11">
        <location>
            <begin position="20"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="12">
        <location>
            <begin position="26"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4365113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="367" mass="40946" checksum="6F421F4BC828B920" modified="2023-11-08" version="1">MRTTTLLSTITLIPSLVTAELFQDVTRSRFEALMQENNLLLTAFTSRTLDSLEPFHDTFTKAAANVSTPLVTIDCDKEIELCKEQDINAYPTIRLMAKNDGQEVDVTRYRGRRTKNAIRSFATKHELPVLIHIQPHDFQALKTIDDIVVVAYLKPDQEELLQTFRDVAESHNQKFVFGYVTDAAIADAEAVTVPSIVCYKNLDGDNKSLSGHFSEADVESFLIAASTYAVKEFRERDMEDFMLPNKLTTYIFTASEDDAIAVRHELTPLATKYDKFVTFAITDTHEYAAMAQNFGVNMDLALPALVVHAPVNDNIFSYQQGKRMVADVVEAMLTTILHGKATSGQVFGGEAPDMEEDIGSSTIHSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y369</accession>
    <name>A0A9W8Y369_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009315</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y369"/>
    <dbReference type="OrthoDB" id="1046782at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359:SF37">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="266" mass="30873" checksum="44287A9B5BFCB911" modified="2023-11-08" version="1">MYSGDDVYFAIKQLQKNDDTSSPPAHESVQEVQAYKRLNQVQDPHLIRLLATFTHRDHLHLIFPYATGDLHNFWYDQFPTSMKPRRDHRFVRWMSRQIVGLAQALEKIHHCHVQRVVHPNGSIDAAKTHGRHGDLKPENILWFQHESDGSEYGSFGVLKISDFGTAEFHSPISLSGETAGVGITPTYKAPEFDMNRISPKYDIWSFGCILLQFVVWYLHGWDGVEDFSEKRMNESNRYDHLDDFFNFYDTDTGKLVTRAKESVRQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3B5</accession>
    <name>A0A9W8Y3B5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">60S ribosomal protein L12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">RPL12</name>
        <name evidence="8" type="ORF">N0V83_008322</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3B5"/>
    <dbReference type="OrthoDB" id="1478556at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.250:FF:000002">
        <property type="entry name" value="60S ribosomal protein L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="7">
        <location>
            <begin position="13"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="6">
        <location>
            <begin position="74"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4365702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="177" mass="18990" checksum="88FA7FBA2A205B7F" modified="2023-11-08" version="1">MPPKFDPSEVKVIHLRATGGEVGASSALAPKIGPLGLSPKKVGEDIAKATGDWKGLRVTVKLTIQNRQAAVSVVPSASSLVIKALKEPPRDRKKEKNIKHTKSVPLEEIINIARTMRFKSMAKDLKGTVKEVLGTAFSTGCQVDGRSPKAISDDIESGEIESTFCIGPQDKIHANRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3D3</accession>
    <name>A0A9W8Y3D3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_007440</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing (1-&gt;4)-linked alpha-D-glucose residues with release of alpha-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.20"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.20" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3D3"/>
    <dbReference type="OrthoDB" id="1334205at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004558">
        <property type="term" value="F:alpha-1,4-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06595">
        <property type="entry name" value="GH31_u1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF89">
        <property type="entry name" value="ALPHA-XYLOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="5">
        <location>
            <begin position="193"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="6">
        <location>
            <begin position="505"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4366911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="833" mass="94781" checksum="B2CE91A748EB81E2" modified="2023-11-08" version="1">MTKPARYDFKTRPVAHPDAIVAGDKYRFTILTDGLLRFEWAADGRFEDRASTFAINRKLAVPDFYVWDRGHGIEIVTKRFHVVYDKRKFSAEGLKIHLKGSVTGTWTYGQELSNLGGTTRTLDGANGRTNLGPGVLSREGMALLDDTKSMLFEKDGWIAGRSNGDRSDQYIFMYGRDYREAMRAFYALSGSQPLLPRYAMGNWWSRYHAYDEKEYLDLHNHFDKDDVPMNVAVVDMDWHLVWNLPDGVNGWTGYTWNKALFPDPDAFMKKLHDRGMKLTLNLHPADGFRHFEKQYPEVARYLGIDPKTKQTVPFDCTNKKFMDAYFDIVHHQHEKRGVDFWWVDWQQGNQSKIPGVDPLWVLNHFHFLDSGRGSQRPLILSRFGGPGAQRYQIGFSGDAIITWDSLHFQPEFTATASNIGYGWWSNDIGGHTHGYKDDELYTRWVQLGCWSPILRLHSDNNPFNTREPWRFNDEARIIVEDTLRFRHRLIPYLYTMNARSASDDEPLVQPMYWDYPEDEGAYNVPNQFRFGSDLIVAPITQPRDKVTHQGVANIWLPSGRYVDIFTGIVYNRKGELQVHRPLHYTPVLAPEGAIVPLDGAWRPKSGSPNPESLEILLVVGADGAFTLLEDDGTGTHVDDGGFLMIDDQGLEQPTTDNVRFSSTPITYKQSTGVIKIGPASPVDHSIPKSRTWKVRLLAHTPASKAEIRCVTTSTATKKHHAKPLSYTITKEPNGTLITLDAVSNQQTCYIELGAPGPQLDVLDPNVKIWDILDKACIEMDRKWDIWNCVNNSEPVLNKVRALQNRGLQQELLDAILELLLADERYAGEGAGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3H6</accession>
    <name>A0A9W8Y3H6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nitrogen permease regulator 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">NPR2</name>
        <name evidence="3" type="ORF">N0V83_007804</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NPR2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3H6"/>
    <dbReference type="OrthoDB" id="338854at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009348">
        <property type="entry name" value="NPR2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12991:SF10">
        <property type="entry name" value="GATOR COMPLEX PROTEIN NPRL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12991">
        <property type="entry name" value="NITROGEN PERMEASE REGULATOR 2/TUMOR SUPPRESSOR CANDIDATE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06218">
        <property type="entry name" value="NPR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="321"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="436"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="334"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="441"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="474"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="484"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="507"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="597" mass="65930" checksum="AC02BBFEAE281AF6" modified="2023-11-08" version="1">MPTRAIKAIFYTRFHHEKGNRVLHQVPSGSITPSTLPSALPAPLFPFASVTQYLIPTQQFCDRLLTFCVNHHRVIGYPVCIREGKYSRNEFIFNFAVVIGENELDWACYGEVVRKLGGLLRGLEEQGGFLSREEEGVWEEDDNGAPRKDNEGLGTFGVGAGSKVYALCEMVLEDLNNYAECMIPIDDSNTINLKLFPTRPPPPPILAHHVPLLTISLASLQSPISSDLTLNRILPYIDGVNSISHIAQLADTDLSLTRRAIQHLVYYGCLVLLDIFSFGAIYAPTAEIGGFVVDADVKEECTRYVRVPRLRLGSNAKTIRVGSESEKSRDERASVSSTTSQHSDTAGSAMKASEFDSHIASQKNDNEDENEWHIDHETLITLYTSLRQGLTLKNWVLENLDLLSGIDVRRFITFGIIKGFLYRLHKYSIATSTVLPPAPPTSLQTSAATSIVNPSRDSDTETIRGANKHHHHSSNPSSHLSSHATTPSQAQAQHTNPSFSLYRPHSTAPTSLNPHLASSQLLNPPKTDDLQGLGSFVSSKERDGGLPLLKFLDGMHCFDEICTELGLPEKIVEGKIRGMGRAWVLLFIVNHKEEMGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3J6</accession>
    <name>A0A9W8Y3J6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_007834</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3J6"/>
    <dbReference type="OrthoDB" id="3172332at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052360">
        <property type="entry name" value="Transcr_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36206">
        <property type="entry name" value="ASPERCRYPTIN BIOSYNTHESIS CLUSTER-SPECIFIC TRANSCRIPTION REGULATOR ATNN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36206:SF12">
        <property type="entry name" value="ASPERCRYPTIN BIOSYNTHESIS CLUSTER-SPECIFIC TRANSCRIPTION REGULATOR ATNN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="267"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="293"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4366199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="313" mass="34788" checksum="4F566B06204A65BE" modified="2023-11-08" version="1">MHEPMVLQALLALSSAHKRKILEPAKRARDGLLPDTQEIFLLKQYGGAIKNMQELLADENRPTRPRLLLTVIMCSLFVLLEYMRGNYETGYLHLTSGAQLAKQLSIEPTSETMCEIKLVHFFAQLQNQSDMYRLQKQRRSQSSLSASPESQKTSTALPATTTTIIPTKTALRFANVAEAGSHLDALTESIVHSTEQARMIPLSAKASRRLLAQEHAYVLASFDAWLLAYHATIAAQPSPESEVDRAGWKGLRQRYDMAMEMADLGVTGRPVEEMEDGGDDSGKQKRGGGVGGEGRKFEWVRSGRNEQMNSLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3R9</accession>
    <name>A0A9W8Y3R9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SDR family NAD(P)-dependent oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008563</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4364947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3R9"/>
    <dbReference type="OrthoDB" id="47007at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF4">
        <property type="entry name" value="CHAIN DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G08710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="14568" checksum="4106041E18BE2EFC" modified="2023-11-08" version="1">MPLYQPGLEVPVLEQFSLKGKTALVTGGSRGIGRQIVQGLAEAGADVAFTYRTATEASETAASIAERTGVRARAYYADVTERIALGETMDRIAIEFANGHLNIVIANAGVCQNVPSLEYDEEAWSHMNSVNYDGAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3Z4</accession>
    <name>A0A9W8Y3Z4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008108</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3Z4"/>
    <dbReference type="OrthoDB" id="809632at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05288">
        <property type="entry name" value="PGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000121">
        <property type="entry name" value="Prostaglandin reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF7">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="2">
        <location>
            <begin position="20"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="354" mass="38755" checksum="5B3254BD977199F8" modified="2023-11-08" version="1">MVQNKGLIFKKVPEGWPKPGQDLTIEDRPIDLNQKLPEGALIVKNYYASFDPYQRGRMRAPEVKSYSPPFELGKPITNRSIFKVVKSNSDKFSEGETLITRGITPIEEYSILDKDAVAICSKLDNPYNLDPKFFLGPLGMPGLTAWSSFYEIGKPKKGETIFISAASGAVGQIVGQLAKHEGLTVIGSVGDDAKLDFILKELNFDSGFNYKKEKPADALARLAPNGVDIYYENVGGEQLEAAINAMNNFGRIVACGMISQYNAKPGEQYPIRNLMSVVAKRLTMRGFIVGDADMGPKHAKEHMEKLSKWLSEGTVKTKISVVEGIDGAAEGLVGMLAGKNFGKTVLQIADLQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y467</accession>
    <name>A0A9W8Y467_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y467"/>
    <dbReference type="OrthoDB" id="542013at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF35">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE FAMILY PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="340" mass="37955" checksum="62013289CAA65CED" modified="2023-11-08" version="1">MSAKLEKQAATHASVLHNVKRQVFQSVPQLPSDISLVGKTALVTGSNVGLGLECARYFLKLRPSRLVIAVRSIEKGETAAKSLRTEFPDAKIEVWQLDMESYRSVQAFAARCEKELDRLHVAVLNAALAKIKLERVKDGRQREVTLQVNYLSTALLAILLLPIMKPTSYSPEPARLTIITSDASLGEKLQDPGQTSLLDSLDQPQRYDGFTQYARSKLLITMFAARLAETVDPDDVIINSCNPGATKGTEFMREGSWVLKALFALFFSLVGRTAVDAARIYVHAALVLGKESHGSYTDWLVRAWPVMMYGEQNRKIQKKLWDETMKELSFAKVNEVLKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4V0</accession>
    <name>A0A9W8Y4V0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4V0"/>
    <dbReference type="OrthoDB" id="10670149at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="484" mass="53790" checksum="82723E3DB4DEA3EE" modified="2023-11-08" version="1">MASNNGLSHSAATVDTTPLSASTVPTAAASTHDLKNVKAALSCVNIGRVLEEICQGGSNNELLRLIGPQVPTQFRKRALIDLRAPLRRIQHVYAICWPKPQAAFNLLLKSLCAVLEQHAAYQPGACHAATYKAEIKVLKDELQTLVRGRPACQDPQGFLDMIARWEILVKEATALAPDAMDAEQTSTEDTAWNPPMNAVVETADQRAGKMDSEPTSAQVVPRVSTPEDDIDLSEEEVKALHPERPKSAALSRLPYTLRRQALAYMCKRVKRMNLLAKQKAEVFLQDNQIPLLTHMMEKNILKDMQHDIKDVSAPTPEQIESEYGHRVEKQYEECGVFQKFLRKVKKFHFIFQDIVAWAKKKASNLSSCGHGHQQQKASSSPIDHANARKELLSALYNSISGLNVNISHSHNNGQPILRSNQIEDMSTKLEQVVVGELNKKIEQAPMNGDQQLAEYIGLGNDLLGKLNNSDFFITTVQEYFAMQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5B5</accession>
    <name>A0A9W8Y5B5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006393</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5B5"/>
    <dbReference type="OrthoDB" id="3755437at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4368038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="24925" checksum="DCB27B0FF760A4BC" modified="2023-11-08" version="1">MEPIFPPEVWSNILAHTDDHTVWSSCRLVSRMLRSEAEREFRTSRLHKLKIRWTFSSHVGQVRASSQEIAFELQTTAGSSVKTLSGKGDDNRLVVYGLKLWWDDWYASVADSVVQAFDEGRIEDVGFVQEAILDALGVTEGAYLKRVGEVTGVEVDRLKQKTMRRYVTFQVEGGTCEGSGRVPWKDVDLRRREISVDWRRLMDDVLGEEHVYVENWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5G5</accession>
    <name>A0A9W8Y5G5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_006776</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5G5"/>
    <dbReference type="OrthoDB" id="2151789at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF28">
        <property type="entry name" value="FAD-BINDING PCMH-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040723785" description="FAD-binding PCMH-type domain-containing protein" evidence="5">
        <location>
            <begin position="21"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="6">
        <location>
            <begin position="58"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4368419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="495" mass="54081" checksum="0C3DC1B4EA96CEFA" modified="2023-11-08" version="1">MRVSILYPFSCLLSAALVSGEASEAACDELASVLPNSTFYPEAPTYNASISSYPFLQLRLHPSCIVRPNSPHDVATALSILKDSNCTKFAVKGGGHNANAGFNNVEDGVTIDMQSLNHVEVALGDEVVRVGAGSLWQDVYDVAEKRNLTAMGGRIGVVGTAGFLTGGGISFFSPEHGWSCDAVVNFEVVLSSGKIVNANATSNPDLFAALKGGQFNFGIVTRFDLKAYPATTLWGGRTVYHPNATESLLTAYTNFKMGEFDPYAAGWVTVRYNHTAATFSPISIMWYTKPAQKPGALKQIMDVKPQLMDGKVEAPISEHTRNASRQVVADPERTIWTTTSFSISPRIMHRIHKLWADLVPEVSKQYAYAKPIAELTFQALPGPPRNGSSPNSFGFTADETPEKDLIFLQIIFTFTDAAATEGFEEALKDFLKLIEGVSKEEGVLHRYKYLNFAASFQDPFSSYGGDQKETLKKVARKYDPQGVFQLQVSKGFKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5S8</accession>
    <name>A0A9W8Y5S8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_007169</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5S8"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17502">
        <property type="entry name" value="MFS_Azr1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000196">
        <property type="entry name" value="MFS toxin efflux pump (AflT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000012">
        <property type="entry name" value="MFS toxin efflux pump (AflT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF198">
        <property type="entry name" value="AZOLE RESISTANCE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="331"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="435"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="465"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="499"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="566"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="106"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="39"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4367585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="604" mass="64731" checksum="1CB30CD611C2F67F" modified="2023-11-08" version="1">MHTTDTTASTSTTYTENEKPHVLETAENRPQSVANNDRANVDGKAANREVDGESRPSRDGSTKATEAHETGGEKQIPTAVTKEDGEPDEEPEDDSKYLSGFKLAILTVGLCLTTFVIALDNTIIATAIPKITSVFNSLEDVGWYGSSYLLTTTSLQPSFGKVYTYFDVKYTYLSALVIFEVGSIICASATSSSMFIIGRAIAGAGAAALYSGGMTIIGFSVPLRKRAIYIAALSSMFGIASVVGPILGGAFTDRLSWRWCFWINLPFGAVSLAVVFFFFTNPERLYNHLTVKERLKKIDIAGAIFLICAIVSLLLSLQWGGFTYTWGNSKVWGTLLGFGLIIAVFIAIQIYQGDQATIPIRVFKQQTVLVSCVFSALLSMALYTHMFYLPFYFQAIKGTTAEESGIRTIAYLVSITISSIVIGGLITVVGWYAPFMWFGGAVFTIGAGLLYTLKVDSPPGQWIGYQILAGIGAGAGVQIPFVAVQVVTSQNDMPTANACVMFFNSLGGALSISIAQNIFVNTLSKEIPKYVPGFDPKIVANAGATNLRRVVPAQLLPGVLHGYNNAIVTAFILAIATSGIAFFVSLGMEQKSVKGKKIMAGGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5Y3</accession>
    <name>A0A9W8Y5Y3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_006921</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5Y3"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050364">
        <property type="entry name" value="Cytochrome_P450_fung"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46300">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46300:SF9">
        <property type="entry name" value="P450, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="402"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="406"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="417"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4367340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="521" mass="58578" checksum="0B9A35FEA30C3CF8" modified="2023-11-08" version="1">MAPLLTRTDVPYIRGIPEIPGALPVTGHLTQLGDDHATVCEKWWRKYGNSVFQIRLGNTRAVIVSSFEDCKKILVSNPQGLIDRPKLFTFHGLISSTQGFTIGSSPWDESCRNKRKAASTTLSKGMMKNYYNMFDLESYCIARDLNRDSKGGSIEVSIRPYIQRYALNTTLTLCYGIRMDEVYDDLLREILYVGSAISLLRSASENYQDYVPILRYLPNNEKNRRSKELRERRDKYLNELLDKVREMIKKGTEKPCISAAILKDEETRLTGVEVSSICLSLVSGGFETIPGTLVSCIGSLSEPEGQKYQERAYQEIKKMYPDIEETWATDFEKENVPYLNAIVKEAGRYYTVSNMSLPRKTMSDVRWGDAIIPKGTMVLINAQAGNHDVDYWGSDAGTFNPERWLDPPSKSSDPSSLYTEKPTSSTSHLSFGAGSRSCPGATIASKLINAALFRLISLYKIESSTTHPPNTDYVDYNAIKSALVAIPRDFKIKLTPRDGTGDLARTVLRMGGERTRDLYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y690</accession>
    <name>A0A9W8Y690_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Roadblock/LAMTOR2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_006042</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GAMAD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y690"/>
    <dbReference type="OrthoDB" id="9985637at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10779">
        <property type="entry name" value="DYNEIN LIGHT CHAIN ROADBLOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103196">
        <property type="entry name" value="Roadblock/LC7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Roadblock/LAMTOR2" evidence="3">
        <location>
            <begin position="9"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="42"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4368960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="135" mass="14438" checksum="C8E564DA88B23697" modified="2023-11-08" version="1">MALNASDETLSLLTRLSQKPGVQSTLILSRENGTIIRTSGLISKSSSANPNSTLPAANDAVPDSYTNGRKESGIHSAEDVASMVWSFLNAAGSLVDGLDKEDEVKLLRLRTKKNELVIVPDPRFILVAIHDTPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6Z3</accession>
    <name>A0A9W8Y6Z3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">INO80 complex subunit B-like conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005969</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6Z3"/>
    <dbReference type="OrthoDB" id="2021186at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029523">
        <property type="entry name" value="INO80B/Ies2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006880">
        <property type="entry name" value="INO80B_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21561">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21561:SF12">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04795">
        <property type="entry name" value="PAPA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01406">
        <property type="entry name" value="PAPA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INO80 complex subunit B-like conserved region" evidence="2">
        <location>
            <begin position="287"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="400" mass="44124" checksum="6F3394150C99C961" modified="2023-11-08" version="1">MPRLRSRAPPAPAPLTAPSAAPTASSTRPRRGTANQSPVAAVIQRSSPEETRQSIRLTVKSSPGKLREATSGAVPKTGLSRQNIVSGKRASRSSRVVREVDSEEDEDEDEDDEDEAEEVDAMDVDDDEEEDDVDAEGDEDEDMDAEGEEDEDMDEDTPAPRVVVPANKTIAVKPAISITKPQDSVEAKEMAMDVSDDDELSDPDSDLDGEEDDAEGEDEDAEGDEDDEMAAQNDEDMDSDEDLSRDQTPDVTKMTKRQRAIINEDSDGALMALSNEAQKKKHLTAEEHAMRRAEMARRRKNLSEKRNEEEKLDTINRLLKKQPPKRGRRVIQDITESGQEEELEAEKANPLWVRYVQNAKGSQMGIPDEWLQAPAGSLFAGSVQPPSKSPFNGRMVQEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7R5</accession>
    <name>A0A9W8Y7R5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Plasma membrane localization protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">EFR3</name>
        <name evidence="3" type="ORF">N0V83_005769</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EFR3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4370005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="19232at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039786">
        <property type="entry name" value="EFR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049150">
        <property type="entry name" value="EFR3_HEAT-like_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47766">
        <property type="entry name" value="PROTEIN EFR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47766:SF1">
        <property type="entry name" value="PROTEIN EFR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21072">
        <property type="entry name" value="EFR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="231"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="618"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="805"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="866"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1025"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1114"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1295"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1347"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="818"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="868"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="881"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="911"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="988"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1037"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1114"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1135"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1167"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1351"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1364"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1403"/>
            <end position="1413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1520" mass="166195" checksum="D187D1D8519BC92B" modified="2023-11-08" version="1">MHAARQACRPKHQVLVLKCYPKFQKNNADVKANSSELSYLLYYASTRRSKLQKVGDFLDKRATKDVWKGSTGSVQVTLQIVRALIEKCPRDLPLYAGAVLRILRTILHSNDVTMVEESVPTFEALCAHQDPAALAADQNYIRQYEEIVQLYAQFASKDAVKTSKTPISVPVAIRYRKAGLQALKAVASSESLGSETGRQLAAIIPAILLSIHSDSGAYVKRLVIRESEKDEHEKELALRRRHSVSTARTTEEDDGGDPLAASGTTEDADKLAEEEVGVLALQALRNIFQGVNRGQLRLATSAVLKFVGTHVRGPQHPGGHDSGDWATVLMAMICQWAPVQDRYAILVGAVEALVRSPIMEDDLERQLVLATIIDHLLASTINFIGLSVMDVLVGLISHTLLLLQLGGPGSNIMPHHQQTTSVLSEKDLGDQSASNKLAGVVMEIVKEPSNARVRLLETVQKCMADLATHVYYTDQISDMVGAILSRLKPSRLSSISTTAEAIENPAGAIDAVADSVSMQEKTYVDRFFSFETARITALESVKAIIKTANTRRPDGSSASVSRSKVGVSVWEGTQWLLRDPSGKVRKAYVDALVTWLNSEKKKQDLKIVDNYRRKERESDKGGLARRAVSNASPREKSSKSGKNSTFLPLLHLAVYENAIYYVDSEADYLLLHLLLTTLVNRLGVNAARSGLPMIMRLQEDIATIGEPGSKVRMGSLVHGFLWALSIALDFEVSSVGRALHNEISRRNNKSLWVKSIRVPPMPIERIETPHPVPVSIPAETVQSEALHPFDNREALVDKIAEGYSTSLYSPPSSPPGSPGRSMSMSSSLPVIHQPKPPKPAPELPYKVREELLADWTRDVCLAANTKDSSSASMTGSRSGTQYTGTQKNNYLGVNIPNGTLDSGSNSPVPSPRRAQSRPPSTAYGLVGRLASSPHRNHSPSATPGSTSSRRSTVRVDDLRRALSTSGGGAAPRTSYSLRAPSHHRRDRKFSDDNDDRESSASDSLVSVHSLSDASFLTADRAVNPRFSQQPQDGGGPDSPTTPAATLTPRPQTANSMNMNMNMGMGSPPSPTHAQAARESAAYNTMHDVPPVPPIPENLRASPTLAGTCNFADYQSHHHQSDTRDFAMRPKTAPNGGIGAGGSTTGVGDLRMKKSRSVKSGVSRGRGRSGERSARDTNTDILFASLPPVPNLPIISESVLWKNHSDFEKPLLSQQPNIRSNPGTAIAAKDRVLHWKKAFSAHRARVGTDSSTSIASVSNLSGQDEDIEKWIQDNLPSRQRPQERVLSITSLSSTSSCEMYTNTDMSDDEQEPEHENMQAAQPKSTLKIIELIMRKVELKLRDVACRECTGNSTSNSRSGASSISYQGNRKSSQSSGNKRKTRTEGSPPPDDDDDYGPNKRRRGSAATTADSSDSGVRFACPFYKHDPDKYRNRRTCPGPGWPTVHRMKEHLYRSHAQPIFCPICYVTCKSDKELLNHSTTAIHQANHRDAFVENFFAEFKKSFLSLLDRYPVPLSSSSFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8I3</accession>
    <name>A0A9W8Y8I3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FKBP12-associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">FAP1</name>
        <name evidence="3" type="ORF">N0V83_005118</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8I3"/>
    <dbReference type="OrthoDB" id="6512771at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06006">
        <property type="entry name" value="R3H_unknown_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.50:FF:000006">
        <property type="entry name" value="NF-X1 finger transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034078">
        <property type="entry name" value="NFX1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034077">
        <property type="entry name" value="R3H_FAP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR, X-BOX BINDING 1 NFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360:SF12">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR NF-X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="R3H" evidence="2">
        <location>
            <begin position="93"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="423" mass="47277" checksum="99616FB50F720CD4" modified="2023-11-08" version="1">MAPCHFPSLCKEDKPCQHKIFVTCECQRIKQEAKCNASKHGEGNLKKTLKCDEECARLERNRKLALALNVDPEHQNDHVPYSDATLNMYQQNSTWAATQEKLLRLFAADPEEKRLRFKPMPRNQRGFVHSLAEDFGFDSESMDPEPHRHVAIFKTPKFVMAPMRTLAECTRTRQVQQRLTPAPAPTSAATLRPKPSNTTGDPYNAFLIMNPRFALTIEEVNAAVKSVLSTTSFRLELEVNFLPSEAVALKPPLAARLNMPDREMQTLLESVKEPLSQALTLQKIGKLQLARLDASLNVLRKESDIGPGSGWSQVAASKGVPLRQVQRSTPFGNKGGFAVLSLSSTKKKKEKPAEVVEDWEAAEEEEEEKEKLSGPNSGAVSEDEVFPQGLTSKVDEQPHEQPKEEIPLTTQPSLGRWADMDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8J6</accession>
    <name>A0A9W8Y8J6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Lanosterol 14-alpha-demethylase</fullName>
            <ecNumber evidence="13">1.14.14.154</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ERG11</name>
        <name evidence="13" type="ORF">N0V83_005219</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.154" evidence="13"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8J6"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008398">
        <property type="term" value="F:sterol 14-demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd11042">
        <property type="entry name" value="CYP51-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000033">
        <property type="entry name" value="14-alpha sterol demethylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050529">
        <property type="entry name" value="CYP450_sterol_14alpha_dmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304:SF2">
        <property type="entry name" value="24-HYDROXYCHOLESTEROL 7-ALPHA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="470"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4370698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="528" mass="59640" checksum="D29EF9BBCC46115C" modified="2023-11-08" version="1">MGVLAHVAGPLGDFTSNSSRPVVFAAGIASFVVLAIVLNVLKQLLPKKSNEPPLVFHWIPIIGNTVTYGMDPYAFFFRNQKKYGNVFTFILLGRKMTVCLDTIGNNFILNGKIKDVNAEEIYSPLTTPVFGKDVVYDCPNSKLMEQKKFVKFGLTQDALRSYVTLITQEVEDFTKRHKSFKGQKGTFDVTKVMAELTIYTASRSLQGQEIRNSFDSRFAELYHDLDMGFSPVNFMLSWAPLPHNRARDNARETMIKLYSELVRKRRSGEARKDGEHDMIWHLMECKYKDGTQVPEHEIAGIMIALLMAGQHSSSSTIAWILLRLAQNSHLVEELLAEQKAVLGEDLPPLTYEDLARLPLHTQTVKETLRIHAPIHSIMRKVKQPLVVDGTNYVIPTSHTLMSSPGFSAQLDSYFVNPAVWDPHRWDAGNQQKYVDEEEGKDEDKIDYGWGVVSKGTNSPYLPFGAGRHRCIGEQFAYLQLQTILVAFVREFKLRNVGGSKDIVGTDYSSLFSRPLAPGTVEWERREKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8M4</accession>
    <name>A0A9W8Y8M4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8M4"/>
    <dbReference type="OrthoDB" id="3927820at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="229" mass="25041" checksum="7536EFEF658CA461" modified="2023-11-08" version="1">MPGSHNAERPSSSPRRGSHIKNQDEESPSYSLLCNLLTSLTVQSTTTFGMPLPDRNQYSNKESAKVNAGLEFGGGAVEGVTLEEPASFLATNTPAVDIDFSQLVVPIAAPEPKPSPEELVRRLLDPPPHAFVHDVLSMQWVRGVVGGGHFDFRESDERYVLAEESELRLRAGELGLPAVYLEWLGGDDEEVSSNEQGMYLTPEFFNEVRSEDLGAQLAPNKGGRLGPHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9S3</accession>
    <name>A0A9W8Y9S3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA-binding transcription factor yap1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">YAP1</name>
        <name evidence="7" type="ORF">N0V83_004609</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the bZIP family. YAP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9S3"/>
    <dbReference type="OrthoDB" id="5380163at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000067">
        <property type="entry name" value="BZIP transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.100">
        <property type="entry name" value="YAP1 redox domain. Chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013910">
        <property type="entry name" value="TF_PAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023167">
        <property type="entry name" value="Yap1_redox_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF6">
        <property type="entry name" value="AP-1-LIKE TRANSCRIPTION FACTOR YAP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08601">
        <property type="entry name" value="PAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111430">
        <property type="entry name" value="YAP1 redox domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="6">
        <location>
            <begin position="162"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="37"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="105"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="272"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="140"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="275"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="294"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="366"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="389"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4371392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="634" mass="68317" checksum="D84DCFFB0A72839F" modified="2023-11-08" version="1">MAGTNDFQNGAPFYLDATQQDLLLAALASNNQNPNDIFSTGLENNSNHKGSLSNSQFPYPANNLDPTYFTSPQQSTPTTGFDNIGVDESPFVDYIDGDTSFDFDNADNGDLMIGALPGDSPGDGNEKRKSPDDDDEEEEGGGKRREGEDKQAKKPGRKPLTSEPTTKRKAQNRAAQRAFRERKEKHLKDLETKVQELEKASDATNHENGLLRAQVQRLQMELREYRKRLSLNSTGINRTPPVASGFSSLLNNNNSSFSFDFPRFGGLPGTQFLDNSASTKNKPTSKSSSVLGRHNSTGGTGSPANQVNSSTTTSPSNMGNSPTTNGVQRSGSLNGFFGHNNTAMNNASRASTDSVSTAQSRVFQFNSGSSTHSDSPSSSTSPNAQDSSCGTSPEPSHTSPGQQRDTIVDGYVCTGNSEGEVLFCEKLNMACGNPRNPVPRAMSKSDDKPSPAVINAAKSPTPAPALNGIDYFANQNGGQFDPTLFGEYRDTQTAIVGDGDFTGGFFNDAFLNAGYGSPFHFGDTPAVQKPNPLEEIERIQDGEDEVVPGEDINSMLNCHKIWSVSSIESTNFKRLIPSRDKLSSRPDFKDGTIDIDNLCSELRAKARCSESGVVVDHKDVEAALKRLPQDKLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA59</accession>
    <name>A0A9W8YA59_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Hsp70 nucleotide exchange factor fes1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">FES1</name>
        <name evidence="8" type="ORF">N0V83_005473</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as a nucleotide exchange factor (NEF) for Hsp70 chaperones which accelerates the release of ADP. Required for fully efficient Hsp70-mediated folding of proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FES1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA59"/>
    <dbReference type="OrthoDB" id="10250458at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000434">
        <property type="entry name" value="Hsp70 nucleotide exchange factor fes1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050693">
        <property type="entry name" value="Hsp70_NEF-Inhibitors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013918">
        <property type="entry name" value="Nucleotide_exch_fac_Fes1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316:SF18">
        <property type="entry name" value="HSP70-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316">
        <property type="entry name" value="PROTEIN FOLDING REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08609">
        <property type="entry name" value="Fes1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Nucleotide exchange factor Fes1" evidence="7">
        <location>
            <begin position="6"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4369710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="210" mass="23244" checksum="DF0CCCD48273490B" modified="2023-11-08" version="1">MNDPGLNNLLKWGIQNSEASRTEGSATEQPPAKVDVEALQRLMTGMNGPSDAQLMVESMDAIDNDEVKLDDKCTAFENFEQLIENLDNANNMETLGLWTRLIAHLENEESDLRMHAAWCCGTAVQNNIKTQERLLVVGGIPTLVKMATSDAEKKVRKKAITALSSSVRNFQPGLDTVVSHMPSEFKPQEKLDANDMDSVDTLINKLRESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAA6</accession>
    <name>A0A9W8YAA6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Proteasome activator BLM10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">BLM3</name>
        <name evidence="14" type="ORF">N0V83_004780</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BLM10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="17907at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016504">
        <property type="term" value="F:peptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032430">
        <property type="entry name" value="Blm10_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032372">
        <property type="entry name" value="Blm10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055455">
        <property type="entry name" value="HEAT_PSME4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035309">
        <property type="entry name" value="PSME4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021843">
        <property type="entry name" value="PSME4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32170">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32170:SF3">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16547">
        <property type="entry name" value="BLM10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23096">
        <property type="entry name" value="HEAT_PSME4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16507">
        <property type="entry name" value="HEAT_PSME4_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11919">
        <property type="entry name" value="PSME4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Proteasome activator Blm10 N-terminal" evidence="12">
        <location>
            <begin position="21"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator Blm10 middle HEAT repeats region" evidence="11">
        <location>
            <begin position="378"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator complex subunit 4-like HEAT repeat-like" evidence="13">
        <location>
            <begin position="1379"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator complex subunit 4 C-terminal" evidence="10">
        <location>
            <begin position="1878"/>
            <end position="1966"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="13"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="930"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="938"/>
            <end position="963"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF11919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4371561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1966" mass="222711" checksum="A2F9A1F3E2BD59EB" modified="2023-11-08" version="1">MAVNERISQLMGALDSASGPDTSRATSPGGDWRQANGGINGADDRSRPRTFPYFKYLPYQTEDQSEREDSLNTCLKHLYIAVSAGDFAPGAVHWTREIRGWLSLKFDLPRSTRVKLVNLYYELALAPGLDYLVAERFASMFMVLTKRKHYLRPGKDLILDWRPLYRELKPFVLPSESGGSHPPNVKRNIRTLTKMCTFAQLYFDPKEIPAIFEELLPFFSTSFSEGAFVVVGLLNLLLPTHPAPEDMKQLLPQEYLPTFFHLWSLVNRSRLFDAHYIDTLSRLARDSLTAEHVPFSQYGVFTGEQSSQIFTAILRLLEIPVGQATSPYSSTVDLGAGLAVMLDRDQRKHPSTHHIARIIAMSLSPACAEHSDSVLSKLEGLIQAVETFFHPSNSGSWTRPLAQLVYYLADFFVLRWNREHSGEMDVPEDRQLNDAVKRRFVLCLREVTFMGIFSKSDKAVQYALSTLQSLAYLEPNLILPGALQRIYPAMQGLVEVHRTISSIRALHMLSKIMAKTKGFRCHVTTVLGLALPGIDANDLEKTLHTLQYVQGVCYLVPFHDLTKAKKALDGETARDGATTPMEDVGGDTGLAVQWITEQVERLETARGNIEINYDEELSDEEEAMILRSSTTGLNEFLISFLGRVFTLLENLPDASRVRSGSPEEYVVNHLPAAFTPLLAALSPELYDVALDKIANFITNHVIHQARDAIAFICNSLVKINPEKALKRILPHLFAGIRTEIEDIGAGSTRTTGAEVLPRDRALVWNLSILSMCVVHVGDAVLEWKDELFEIAEYMQQRCRGTPTVHVSNFIHHLLLNLTCTYTVDYSIYEEDELANGLSTSSWGRQVDVKKLDIKWHSPSPEEIDFAVKLFETHASSSMKALAALTGPNSPIKREGTGKDWSDEVSRNLVLLRLIISGISVLFDVRHDQVTDGSAESDSGSDSDGMDTEGIDDDAGLGEAEDEEVKPTFQYESGYPLRRDEDNYKLIHDLRQHVGEILHNVHRFLIEKQPDDVPCFNSLYNAYRSWFIDVGIERSAHVLDRISRLLDNDEQPFRFSGLRKQYPRPLLVRRANVYHLQRLRHNANPRPHTELDKLLLLDLAESSISLYTEIRRTAQTANESAVKCILGARPLVIPPLLDAFVKAVAESDYPRIKGAMFALLFGSLAKTISRDWRFTPRLIKAFIEVTGADKPSVQKLATNATFQVMDMGKAMERMVILEPDVVQAIAYAIDSSEDEHVQSKVTKRRDYIKKKRARIEGKKADLSKELVEIANTEHWKKVSRTAAIIVNLGMRYDTIASDEMIDLVSKGAIDQHPSLRGLYAGALIGLFSVIQTRAITGHDYKQYLLGEEDLPDKRTVNTKPDDPNWTQEYLDSFAHPEAKYYVDFDYPGWLVWNKTMTVFLPNAPQVSYDNVENDVRQKIAKHLTRSWFSNYFAFMKQEPRDNSADRFRMSSAMLLTHSFDLVFCGLTEATFEDIKDLTQSVYGDGSDKHQHRATSEIMAALISCAPDLKPEQRQEVWEYVFPIVRGIFKDGLTPENSGYWTTFLHVVLQSKDPRRGWPLIDWLASFRLDMDSNAAFKESSKVHLLTQCICDAGWHFRLEKPILEDFLKHLDHPYKGVREAMGQTIASIHRTRYHESYKDVGTLIKAQKDSSSIGVRPYQPTEEFSKMITEVFDRLEVWRQQRPAGQQTPSSYTSGGKTVLLWLDSTLSSYECTSLVKFFPNVFMEQLLHMMDIKEDPELQSLAYHVFRHLPNIPHREGEDSEFIAALIRIGRNATSWHQRLRILINIQVIYFRRLFLMSEEQQQSMFDCVSAMLSDVQLEVRMGAATTLSGMIRCSPIALRDRQVSTLKEQFTKQLNKNPLPKKRIPGTPTPEQGKLVLTRHAAVLGLGALIQAFPYQSPPPMWLPDVLATLARKAASDPGMVGKSVKSVLADFKKTRQDTWHVDVKAFEQEQLEDLEGVLWKSYFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAF5</accession>
    <name>A0A9W8YAF5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Diacylglycerol kinase</fullName>
            <ecNumber evidence="3">2.7.1.174</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">DGK1</name>
        <name evidence="3" type="ORF">N0V83_004803</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.174" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAF5"/>
    <dbReference type="OrthoDB" id="5673at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141035">
        <property type="term" value="F:CTP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037997">
        <property type="entry name" value="Dgk1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31303">
        <property type="entry name" value="CTP-DEPENDENT DIACYLGLYCEROL KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31303:SF1">
        <property type="entry name" value="CTP-DEPENDENT DIACYLGLYCEROL KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="369"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="387" mass="42511" checksum="B7A5D1738F24E7C9" modified="2023-11-08" version="1">MESSRQYQIPQTPRVISPSPTPSEAGSGKDGYFGPVTRSSTRKNKQRVTSHPPIDEDSSGSDPEKRARARSRSPILAGGVGRRTMSGLTTRRQVNGSAKKDPTLPNGTVNGHLSPAAANKNYWREMSRSPSPLGLIPIHQKWRSFIHRHEIPRKILHVSIGFLTIFLYCTGTQTSQIHNVLLTLLIPIALTDVIRHRYWQVNRFYIRCLGALMRESEVDGYNGVISYLLGAWIVMRFCPKDIAVMSILLLSWCDTAASTFGRLWGHLTPRVRKGKSLAGSIAACVTGIITAVVWWGWLGPLYSEYNHGGNAFAFQGVLALPTQARELLGLSFTQASITSYWALGTMSVAAGIIASASEALDIFGWDDNLTIPVLCGAGLWGFLKVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAJ0</accession>
    <name>A0A9W8YAJ0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">PKH1_2</name>
        <name evidence="2" type="ORF">N0V83_004849</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAJ0"/>
    <dbReference type="OrthoDB" id="347657at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="203" mass="22431" checksum="AA0DAE91C846F864" modified="2023-11-08" version="1">MFFSRFFGGNTIKKRSRLVMITSNARVLMCAAGTTDKKLKEEVSLLSPGCGWRSFQGSKGLTAWLVETVRLQHQFSICIILTCVQRDKQYVFEDPKSTTSDPDGSKYSSQEWLDSIEQARDYAFSQTMSNSAYAGDAALNELGSTLSTPTSTLGEDSALEGVNIPTARHGHGHLRKDHGGDLDSLKGRKRFSKRHSKNGLANF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB07</accession>
    <name>A0A9W8YB07_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">uS12 prolyl 3,4-dihydroxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NUA3</name>
        <name evidence="15" type="ORF">N0V83_004149</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[ribosomal protein uS12]-(3S)-3-hydroxy-L-proline + 2-oxoglutarate + O2 = [ribosomal protein uS12]-(3S)-3,4-dihydroxy-L-proline + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54160"/>
            <dbReference type="Rhea" id="RHEA-COMP:13817"/>
            <dbReference type="Rhea" id="RHEA-COMP:13818"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:85428"/>
            <dbReference type="ChEBI" id="CHEBI:138052"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[ribosomal protein uS12]-L-proline + 2-oxoglutarate + O2 = [ribosomal protein uS12]-(3S)-3-hydroxy-L-proline + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54156"/>
            <dbReference type="Rhea" id="RHEA-COMP:13816"/>
            <dbReference type="Rhea" id="RHEA-COMP:13818"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:85428"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TPA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB07"/>
    <dbReference type="OrthoDB" id="430522at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031543">
        <property type="term" value="F:peptidyl-proline dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009896">
        <property type="term" value="P:positive regulation of catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006449">
        <property type="term" value="P:regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000014">
        <property type="entry name" value="Prolyl 3,4-dihydroxylase TPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.20:FF:000001">
        <property type="entry name" value="Putative oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.20">
        <property type="entry name" value="Oxoglutarate/iron-dependent oxygenase, C-terminal degradation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019601">
        <property type="entry name" value="Oxoglutarate/Fe-dep_Oase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043044">
        <property type="entry name" value="TPA1/Ofd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039558">
        <property type="entry name" value="TPA1/OFD1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051842">
        <property type="entry name" value="uS12_prolyl_hydroxylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12117">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12117:SF0">
        <property type="entry name" value="PROLYL 3-HYDROXYLASE OGFOD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13661">
        <property type="entry name" value="2OG-FeII_Oxy_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10637">
        <property type="entry name" value="Ofd1_CTDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="14">
        <location>
            <begin position="136"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="491"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="553"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="500"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="558"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00081607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4372375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="649" mass="73493" checksum="3E1E66D2921576B8" modified="2023-11-08" version="1">MPKRKNEAPANGKATKKRAISDDEAHQNFHKGLFDSKVLDGYTNYYAESQPYKHAVIQNLVNDQLLRNVRNEIREHISFTPKETDIYKIHQSGDLANLDGLDDSALKRLPSLLTLRDSLYSSAFRKYLSSITQSGPLSGVKTDMAVNVYTPGCHLLCHDDVIGSRRVSWILYLLDPDIQWKPEWGGALRLYPTEHLKTPDGDEVKVPQPDFSLSIPPAWNQLSFFTVQPGESFHDVEEVYRRSVEEKIEEDGGRIRMAISGWFHIPQEGEEGYEEGLEEKLAEKSSLAQLQGGKADIFDEPQAQWVDNPDWEEQAKQEEDDELTENEIDFLIKYMNPNYLTPDTVEELSKMFSDESSLRLAEFLSNKFSARLRTYLESKDHEKTPSLPTNPYHKDSKVGVARPPHKHRYLYRQAIQPIPTTPYPENDAMTPYDDLVDVLFPHPAFLKWISLVTGITLTKSNIFARRFRRGLDYTLATAYEEEDPQLEVCLGITPSKGWGDEDEEPEEEEAPQQNGNSSSSSKSKKKNGKAPVPEPKPEEEVGGYEMYMAGEDADDVPNVSSEPSTSTGAGQRRKAKADPAIYKSAADDEDDGILFSQPAAWNNMAIVLRDQGVLRFTKYVSRSAKGDRWDVCAEYGVEFGEDEDDDEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBB3</accession>
    <name>A0A9W8YBB3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_003376</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBB3"/>
    <dbReference type="OrthoDB" id="10262656at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF6">
        <property type="entry name" value="MULTIDRUG TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G08740)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="336"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="374"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="449"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="493"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="519"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="44"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4373085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="553" mass="59820" checksum="DD5A016C999A03D0" modified="2023-11-08" version="1">MGAQDRLKEEERLLGDIVMEEKGVSLNAQEEEKATWTNLPHKKQLLLLSLCRLSTPLSNACLLPYLYFLVKSILADPEHHTATQQISRLTGLLVAAYPVGQMMTSMLWGRLSDIYGRKPAILLGLTISVVANLAFGFSRTIGMLVFWRVLAGMANGILGVMRTMTAEIVKERKHRPRAFLAPPVIFNSGRVISLAVGGCLADPVENLPSLFGPRGLFNVSKNSDGVKWALQYPYALPAIFNGAVLFVCLVLATFWLRESLSEKENHKDLGLALGEALSGFIKRKVLRKPASGYALVQVEETEAVRPSISTTPSSAGFSTPTNFTSRPLLRDIWTPLLVKTLVAFALLPLHTATFLHVFPVFLSMPTATSSSSSMFHFIGGLGLASPTIGLYLATFGIAGILLQLFIYPRLQSRIGTLGVFRLASLIFPLAYISAPFLALASGYGPAKYIAMAAVLFTQIIARTMAIPSSVILLTEVAPHRSVLGTIHGAGNTLSAFSSACGPAIGGVLLAKGIEMEAVGLVWWTWLSVVSVVTLVWSFVLDKSQQDGDICESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBG0</accession>
    <name>A0A9W8YBG0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Multiprotein-bridging factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MBF1</name>
        <name evidence="10" type="ORF">N0V83_003293</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Transcriptional coactivator that stimulates GCN4-dependent transcriptional activity by bridging the DNA-binding region of GCN4 and TBP (SPT15), thereby recruiting TBP to GCN4-bound promoters. Involved in induction of the ribosome quality control (RQC) pathway; a pathway that degrades nascent peptide chains during problematic translation. Required to prevent stalled ribosomes from frameshifting.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MBF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBG0"/>
    <dbReference type="OrthoDB" id="10253401at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000030">
        <property type="entry name" value="Coactivator bridging factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013729">
        <property type="entry name" value="MBF1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10245:SF15">
        <property type="entry name" value="ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10245">
        <property type="entry name" value="ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1 MULTIPROTEIN BRIDGING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08523">
        <property type="entry name" value="MBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="9">
        <location>
            <begin position="104"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4373002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="160" mass="16899" checksum="4CAE18DD28A52BD9" modified="2023-11-08" version="1">MSDDWNSVTKIGSRTRGGAAGPRETVVKGKSALNAAQRSGGIVATEKKFASSNAAAASASQEGQRLTKVDRSDDIVKPKTVGTVVGQAISKARSEAKNDKGTTMTQKDLATKCNSTPTIIADFERGTAAPDQKLLGNMERLLNVILRGDKIGQPKFPKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBW5</accession>
    <name>A0A9W8YBW5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBW5"/>
    <dbReference type="OrthoDB" id="2279190at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="149" mass="15812" checksum="BF5FFD1F75D0149D" modified="2023-11-08" version="1">MLPRPTAKYYLKPTILYLSCPEDLAWPASSPKRTKSKSKTKSTPEPETAISLEPNMADKTKDTLNNVTGNAPGGRGLGEVGDSIGPIAKTPGVAGSDSREGNKTKNADEGFGLDNKDVKGSLKVHIKLDLEADIRVIARIKGDIAIGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC12</accession>
    <name>A0A9W8YC12_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_003546</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC12"/>
    <dbReference type="OrthoDB" id="5511210at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="5">
        <location>
            <begin position="237"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="17"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="91"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="124"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="181"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="237"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4373252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="357" mass="38701" checksum="1D6D10733B2E06C3" modified="2023-11-08" version="1">MPSVTYMNQSAPFWDFIASLEQQGPQATSRHNHDDENEQEHEHEEEHHGPPANFGPWGWGPFFGGPRGMPHRGPPHGRRPHHHDEEAGENNQERTGEEDPEKNENDNGEGPSGNSGSDNEGRFHGRGRHGRCGGRRGRCGHGGPGGFGGPGGRGGFGGPGGRGGFGPRHGPPHGGPGHFGPGHHHGRHHGGHGRWGSWGRGGPFGFGGNSFDPSALAAHFWEQFTENQPSNKKDNDNDNEDYTPDADIFDTENAFVVHISLPGAKKEDVGVNWDAEKSELSIAGVVYRPGDEEFLKTLAMNERKVGPFERKIRLGTRANPASVDADSITAKLEDGVLRVDVPKLDSGYVEVRKVDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCJ5</accession>
    <name>A0A9W8YCJ5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulatory protein sin3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SIN3_1</name>
        <name evidence="2" type="ORF">N0V83_004283</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCJ5"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="158"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="256" mass="27555" checksum="B168C526360C4C9B" modified="2023-11-08" version="1">MGAGYYSSNAATGHNQNLPALPGLPAPAPQSSPHQSAQRPSSSDVGAQSQQGPQGPPYSLPGISQTLQQQQHLSSEQANADRERELRERETREREMMESHALQHAAQQEEIVKREAEQREREIHERQQREQAALQNHSAPIQIHQPVAVAPSTRTIHGPNGLLGQSGPLGGPNLAPPMGGPNNNNAPMYGNTSAQHDQNTPRIPHAVQPPSQAQMLMPFVGPPGAMGMGQGQQPILNVSTASNLRGSRHANDHRMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCN4</accession>
    <name>A0A9W8YCN4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pantetheine-phosphate adenylyltransferase family protein-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCN4"/>
    <dbReference type="OrthoDB" id="330671at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695:SF46">
        <property type="entry name" value="BIFUNCTIONAL COENZYME A SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695">
        <property type="entry name" value="DEPHOSPHO-COA KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="412" mass="44887" checksum="6F35945408E9A586" modified="2023-11-08" version="1">MPPTLKQSHRALLLLPPAPSPPSYAALKAAYHAPLHTVLSGLARSSRSAQEPALLDIALPCPHLYGRLDAPRGPLYATTQQLVADLYKLICITATQEAIDTEDAEGVDARVILVAYPRNGQLTTPLQDATPEQELQGPAVDIHALATSPRSWDAVYSVESEEGEAVLKNFLSLSSSQRSVSRVRGGIVRVESSTPATPATETSNVSTNHLSVAVGGTFDHLHIGHKLLLTMFAFTLGRSSASTADPKPSTLTVGITGDALLKNKKYAEYLESWKCRQESVHDFLSSLVYFGRPDDERIQVHEINEAGPNGHAVNVSYPFGLTIRYVEIWDPFGPTITDKDITALVLSLETRSGGAAVNNKRKEQGWEPLDVFEVAVLDASEEDNVDETFQSKLSSTEIRRKRSERIQSRSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCV2</accession>
    <name>A0A9W8YCV2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 11">Glycylpeptide N-tetradecanoyltransferase</fullName>
            <ecNumber evidence="5 11">2.3.1.97</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">NMT1_2</name>
        <name evidence="16" type="ORF">N0V83_002833</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>N-terminal glycyl-[protein] + tetradecanoyl-CoA = N-tetradecanoylglycyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15521"/>
            <dbReference type="Rhea" id="RHEA-COMP:12666"/>
            <dbReference type="Rhea" id="RHEA-COMP:12667"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:64723"/>
            <dbReference type="ChEBI" id="CHEBI:133050"/>
            <dbReference type="EC" id="2.3.1.97"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the NMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.97" evidence="5 11"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCV2"/>
    <dbReference type="OrthoDB" id="60315at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004379">
        <property type="term" value="F:glycylpeptide N-tetradecanoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000042">
        <property type="entry name" value="Glycylpeptide N-tetradecanoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000056">
        <property type="entry name" value="Glycylpeptide N-tetradecanoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000903">
        <property type="entry name" value="NMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022677">
        <property type="entry name" value="NMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022678">
        <property type="entry name" value="NMT_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022676">
        <property type="entry name" value="NMT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11377:SF5">
        <property type="entry name" value="GLYCYLPEPTIDE N-TETRADECANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11377">
        <property type="entry name" value="N-MYRISTOYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01233">
        <property type="entry name" value="NMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02799">
        <property type="entry name" value="NMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00975">
        <property type="entry name" value="NMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 16" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycylpeptide N-tetradecanoyltransferase N-terminal" evidence="14">
        <location>
            <begin position="170"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Glycylpeptide N-tetradecanoyltransferase C-terminal" evidence="15">
        <location>
            <begin position="339"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="32"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4374094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="557" mass="62591" checksum="0D70D019B3C1E675" modified="2023-11-08" version="1">MPQEASKVSDPDATAEAAAEALAESSKQPEAESADESGDEATEAVEGAEGAAEKKKKKSRKRKIKDALAGKGKEPATTNAPAAGHLSKDQMSQLLDANPALKNELLGKAKDKGDLEQMIKKLNINELLTGLAPQGSTKDMASHAFWKTQPVPSFDEMANKEKIKDGPIKEIDIEKVDKNPSPMYPGFEWVTMDLEDEKQLDEVYELLTNHYVEDKDATFRFKYSPSFLNWALKAPGWKKEWHVGVRATASGKLVAFISGIPIQLRVRENVLNCSEVNFLCVHKKLRSKRLAPVLIKEITRRCYVEGTFQAVYTVGSLLPTPVSTARYFHRAIEWEKLYDVGFSPLPHGSTKQRQIIRYKLPDTTSTPGLREIEAKDIDATLDLLKRYLARMDMAQVFSKTEFEHWICPNDKPKEQVVWSYVVEDPESKKITDYFSFYNLESTVIGHKKHSTIKAAYLFYYATEVAFEKKGDEKELKTRLNALMKDALILAKKADFDVFNALTLLDNPLFLEEQRFGAGDGSLHYYFYNYRAAPIPGGIDSRNQSSTKHMGGIGLTML</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD94</accession>
    <name>A0A9W8YD94_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PXA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001998</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sorting nexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD94"/>
    <dbReference type="OrthoDB" id="41200at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000065">
        <property type="entry name" value="PX domain protein (AFU_orthologue AFUA_2G07450)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003114">
        <property type="entry name" value="Phox_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013937">
        <property type="entry name" value="Sorting_nexin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF47">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 122 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08628">
        <property type="entry name" value="Nexin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02194">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00313">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51207">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PXA" evidence="3">
        <location>
            <begin position="168"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="371"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="421"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="707"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="374"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="429"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="488"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="725"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="766"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="797"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="814"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="874"/>
            <end position="886"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4374920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1032" mass="112212" checksum="9CFF7B3366B12EC8" modified="2023-11-08" version="1">MSEPVQAPPSLHDSLSEKPHDSPVRNVAEQPESKQARDSSPGLEVKALTDRALHFLATASNETLGACLAGLGAGTYLILGRVGLVLIGVVGGVVLHATWEGRTQDADAGENKAKSAEARRRELGVDIAHRVLDWRDTRAPEGSKDGDDGQDLNLMLYSGKKLDYSDFKPETAAALTELTEAVIRDYVKWWYSPVLPTEESFPNSCRQTFTAFMLSVSAHLSRKRPADNFVDFVTRSSSFMIILLQEISAAIQASPGDAPTEAVDIYLKMKRDSTLANILDPDYQMKQFSVAAEDILQNYLEPKTYNCVPARIFLHQVLAKVILEMIVVSCSKPEWINGWIVYLLEDGEPELMEAIDAGVEGSAGQLENVKKVVESQTRASQQEPSAERREHKRAMSRAQEAMDDAMQEAQRLSQMIAEEDAKRLKEQAKQSASSSTLNDDQSESTTQGIVTPTSSQSETAAENEFTDLGISGLSMSTSSQIAPASPPHEVEPSPKQEVRKAFTSFDQLVPNNPPTALIDTPQPMESPPLLTLHNSTMAIFDDSMPGEKGTIKNKPTAEYLIQIEPKSHHHSGWMTARKYTEFETLHEVIRRIAAITGTTGFTEIHATLPAWKGHTKASLRGELERYLNDAVKYKPLAESEGMRRFLDKELGSGKSSGGGFPGIGWAGQTFDNMGKGMMDVLTKAPKEVAGGGKALFGGVTGVLGSVTAPLGGTKKNRDSVPNAGTPSPSNNTPNPSRTSTSTAPIQTRHSRAESTVSELPSHIRSESTLSLATKRTSIDSVRNPNSPVIDQQPQREAPMERRPSYNPDGDGKRSGMSSRYASRSNSRAPSVRESIDDLSPVLGGDQILNLPPIPSEISDDYGAGSPWRNPPDPMQSSRASTTSLPPSNDAVPPHTPLSEPETTVLIELFFAVINELYTLSSAWTLRRTLLNAAKTFLLRPGNPQLSSITTLLQTTVLDNNTSDAGLAYHLRKMRANSLPTEEELKSWPEEMSAEAKEKLRAKARKLLVERHAAGVDECYGAGGEWGGVGEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDA6</accession>
    <name>A0A9W8YDA6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002762</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDA6"/>
    <dbReference type="OrthoDB" id="2384350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17716">
        <property type="entry name" value="BRCT_microcephalin_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="706"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="827"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="148"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="200"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="832"/>
            <end position="844"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="939" mass="100107" checksum="D7248427E02BDC33" modified="2023-11-08" version="1">MVATRRGAKKAPEAAPAPSNTLNAPPKRGGRKKAVVEEAAAPAPAITKPTKTTTAKRKAVSEPEDVELPVAKKPAANTKATRTAKVETKAKPAPAAPKRATRSRKVEEPAAVEEPVVVQEVPKPAATRGRKAPAKKTPAPKVEEPTAIEEAVAAEAPKAATRARKAPAKRAPATRKVDLPVIDETVAAEPVKPAPRGRKAAAPAPAIAGRATRGRNVAAPPQDSPLKAPARKPTKKAAAVAEAKAEPEPAEEPFAQFPGYPTTPAHIAAPLTAKQAMSELPQYPNTPAHILAPISNKDALSVMPGYPKTPSHIVAPMITKAALAEMASYPKTPAHIKAPVSAQVAMAEMPGYPKTPAHIAVQGAVNEENVMDQLPDYPNTPAHITALINNQEALNELPDYPKTPAHIQAPLSSRKALAELPDYPKTPAHIIHAAPVSSGLEEQSMTPAPVTPPHNHEVVDNVAIGASTPYQNSPPVVEVTHEFSEASEGPANITWGVTDQEAFEELPAAYPTTPAHIAAPVTTKEALAELPEYPKTPAHITAPITPRRALAELPDYPTTPAIAIEAAIQEEITASVKKETISPLQFPSLENVSFHFAATSGHSDSVLMDIDSNALGTELTPNMHLTPLQLTSGYSDSVTMDIDSNALGTELASNMHLAPLQLTATFQVPELASPMKSALRSPQKMDAKTPKKAVTWDDPEESDLFLYDGLLQGMTFYVDVTSNGREQNYLFSSLLEDLGAKVVSDFAVKGISHVLYKDGNNSTLKKVLASKGAIKCVNVGWVLDCEEKRMRMDETPYLVDLSTAIPSSPIPTTTMKPFTPARTPSKYALPPSQCNSAPTTPTSSEFDRSFNHDDKENCELGIFFDAPDDMKPAPRTLPHKKSTFLFSRSPIKTPSKPSFLSNTPVKPMSAMPFSTSKKRSAESVFTSTSIGPPKKLRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDB3</accession>
    <name>A0A9W8YDB3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peroxisomal adenine nucleotide transporter 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_001879</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDB3"/>
    <dbReference type="OrthoDB" id="18574at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015217">
        <property type="term" value="F:ADP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052217">
        <property type="entry name" value="Mito/Peroxisomal_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939:SF2">
        <property type="entry name" value="CARRIER PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G13870)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PMP34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="41"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="145"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="248"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="444"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="282"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="446"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="485"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4374802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="539" mass="58067" checksum="D2C9040EC53033FB" modified="2023-11-08" version="1">MASNDTPDKTNYVYNSQLDAFTLYHISNETGRSHSAIGPGLPALGHALSGAAGTAISKLITYPLDLVITRLQVQRQLQHDGSSPHYNGILDAIEKIYEREGGLPAFYSGVLQETMKGVTDSFLFFLAYSYVRQKRLSTRDGGKSLPALEEIGVGVVAGAFSKFFTTPIQQIVTRKQTAAMMKHNSTTSIPPLTNTRDIAREIHREKGISGFWSGYSASLVLTLNPSITMLLHKILLRLLVPRANRDAPGARITFLLAAVSKAMASTITYPFSLAKTRAQVSAQKPSATSGETSETDQPGKFLDSGAARARQRTVFSTILRIAQTEGLWGLYQGLGAEVLKGFFSHGITMLMKDRIHTAIISLYYSVVKSLQRYPSREEITKTASDSVKNAYESSKEQINGAYAKGVEFAGNASEKVKDAVAEGSQQAQSLVEKGTEQANDLYSRGAQAADTASSSAQDALQRGSQQAGSLLKKGKEQAGELAGWASSTTQDAAANSSQRAGELLDRGQETAGKVVEQAKDRLINTDIKDINGPDTGIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDR1</accession>
    <name>A0A9W8YDR1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_002967</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDR1"/>
    <dbReference type="OrthoDB" id="5337438at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001827">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="3">
        <location>
            <begin position="195"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="120"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="404"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="564"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="407"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="429"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="445"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="579"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="596"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4374226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="607" mass="67203" checksum="7F1CC7A541C74EE6" modified="2023-11-08" version="1">MASTTPSRSPNPAKEGSPDPSVRPSIAQSNGSDQGGPRRSSFNFLRGSNSRSVSGGKMSKKQKALAQEEALRRQREAAMLPKQPPRLPSHSALPQIETFGGEGTRPDSVAIVSNKVGNYTHNSHNFSRPSVDRGSRATPQPNLAAAQPMTGSSPSYMDYDPYPRTESMTHRGRYSYASSQHSTVNSPRRVRRRKDPTPFNILVVGAKGCGKSSFIDFLRTALALPVKKRPHTPSPPATAVGGEDSPFTSEYLETEVDGERVGVTLWDSEGLEKNIVDFQLPIITSFLESKFEDTFSEEQKVVRAPGVKDTHIHCVFLILDPARLDTNIIEARKKSNHGKVGSTIFGGLDENLDLNVLRELQSKTTVIPVISKADTITGAHMRHLKKTVWDTIKRAKLDPLEALNLGLDDDEDPDRLDERDEDAYQSSEGEDKPDLLNKILERSSSEAARSIASDNSSSKSQSPPTSPPSARKNHSRKPSAISISIQKEDSETPFLPLSIISPDPYEPEVVGRRFPWGFADPYNPEHCDFVKLRESVFSEWRGELRETSRELWYEGWRTQRLEKGNSRRRVVTSDGPASRQLSVPATNGRASSAGSREYRQQRTSDFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEA4</accession>
    <name>A0A9W8YEA4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pentatricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_003577</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Regulates mitochondrial small subunit maturation by controlling 15S rRNA 5'-end processing. Localizes to the 5' precursor of the 15S rRNA in a position that is subsequently occupied by mS47 in the mature yeast mtSSU. Uses structure and sequence-specific RNA recognition, binding to a single-stranded region of the precursor and specifically recognizing bases -6 to -1. The exchange of Ccm1 for mS47 is coupled to the irreversible removal of precursor rRNA that is accompanied by conformational changes of the mitoribosomal proteins uS5m and mS26. These conformational changes signal completion of 5'-end rRNA processing through protection of the mature 5'-end of the 15S rRNA and stabilization of mS47. The removal of the 5' precursor together with the dissociation of Ccm1 may be catalyzed by the 5'-3' exoribonuclease Pet127. Involved in the specific removal of group I introns in mitochondrial encoded transcripts.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Binds to mitochondrial small subunit 15S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEA4"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF17">
        <property type="entry name" value="OS12G0638900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="5">
        <location>
            <begin position="453"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="5">
        <location>
            <begin position="488"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="5">
        <location>
            <begin position="634"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="5">
        <location>
            <begin position="787"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="30"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="563"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="81"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4373283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="872" mass="96741" checksum="E20F96D44EB12401" modified="2023-11-08" version="1">MLGSYVCRQCRARLSRRAVPIRTPRWQSRATFLSLRNPKPQDDAEPTKTTEQSPIQSQPEAATDEYRHGPRIQHMSGGGPQRERRAGRYSRLVDDSTESETQTLPTYLENTENGNIENVQDKLPLEAGDSPAQSIDAALNSGRVGHAWSLFEKNYTSRDCEALTDPALGDLPLLNNGKVFMHLLNSVNAAFCKGQTNVAATPTLALFKYEQLGVARPEYWARQTLSYLTYQAIQAVNATPKKPQRDLPSLLSELLSVWQLFFQCLGRNTGPLETVSTEWQLPSSDALPEMYESKGFSMRLQEYHPRYIGNPTLGFCAVYLYTISDALNSIDSLRQQAAPFLQFLHRLLAGSYVNPVFKHTEQSKVFHGLPVEVQKQIRKEIDGAPLKAMAMIGSKGETLGPEDTGDAAANLEAFNLKRIARAVESLASAPMLDNLWKGVEEAYTPQGQKAAIPPRIYNAFLSGFMVLSQPQRSVDVWNHMIAHGIKPDLLSWVALLNGCAKAKDLNGFNAMWTRMLNTGVEPDNYAWTTRVNGLIALRQVNQGLVALDDMGKRWLSAENVINNPQTHGKNRKGPGNLPKSSKAVNKCPKPSIEVVNGAISALVQIRPGSMRDDKRIEFVQKILLWARNFDIKPNAITYNSLIQLYLRAGDQGTAFKILQQMENDGIEGDIATHTMIITAAFQNHSFDTLSPAAQTDRIISLFSDLEAGGMKLNDYVYSTAIDRLLKQYGNHDAVRALIDHMSARNLVPSAHAYTSLITYYFQQSPPAIPAVDSLVLQMFTSHRMPSDRIMFDRVLEGYAQHNEVGKMMSVLTKMSKHGKLPGWGALTAVVKALVMDGDVERARAVVRDVERGGRCAGGVQGVGLGRGRFSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEJ0</accession>
    <name>A0A9W8YEJ0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">chitinase</fullName>
            <ecNumber evidence="3">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">N0V83_001251</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Dual chitinase/transglycosylase that plays a role in cell wall architecture. Chitinase and transglycosylase activities are coupled. Required for the polysaccharide cross-linking at the septa and the cell wall. More specifically, transfers chitin to 1,6-beta-glucan in the cell wall.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the glycosyl hydrolase 16 family. CRH1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEJ0"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02183">
        <property type="entry name" value="GH16_fungal_CRH1_transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000152">
        <property type="entry name" value="Cell wall glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF27">
        <property type="entry name" value="GLYCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GH16" evidence="15">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="253"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="428"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="326"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4375971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="482" mass="49065" checksum="91C3CC9E98BB6FEC" modified="2023-11-08" version="1">MEKTCPNDPAMPQTWNTEFTAGKDAIKGWKQTAGSLTYGSDGAEFIIAKKGDAPTIGSESYLHFGYVEVTMKAAPGQGIISSIVLQSDDLDEVDWEWIGGVDSKVQQNYFGKGNTTTYDRMIEADVANNQNEFHTYALNWTAEALTWIIDNKPVRTLKYADANGGKNYPQTPCNVRLGNWAGGDSVDAGTVEWAGGKVDYTKAPFTMTVKSIKVINYSPGTEYKYKDKSGSFESIEVIGAGNAEGAPQNTDVIQSSASATGAPLASGVDAPSATGSAAQGSGSSPSNSTSTTCTEGENATTPAATGSTKSTAAPGGGESGFDYPSVPQTSEGAGSPPAEQTPSPQTSGSAGGEESAPCECGTATVTVTGAPPPASSAPPAVFTSEFSAVPLSSILTRVSTPAAAQPPTTPVVSQPPYPTTGLAIDTRSAPAVPNPSAPTGALPVPSGNATTSAPPAEFTGAASHNKASVFAGVLAGAMLLAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEU3</accession>
    <name>A0A9W8YEU3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">nicotinate phosphoribosyltransferase</fullName>
            <ecNumber evidence="3">6.3.4.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">NPT1</name>
        <name evidence="11" type="ORF">N0V83_002639</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + nicotinate + ATP + H2O = nicotinate beta-D-ribonucleotide + ADP + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAPRTase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.21" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEU3"/>
    <dbReference type="OrthoDB" id="193380at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004516">
        <property type="term" value="F:nicotinate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034355">
        <property type="term" value="P:NAD+ biosynthetic process via the salvage pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.140.10">
        <property type="entry name" value="nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041525">
        <property type="entry name" value="N/Namide_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040727">
        <property type="entry name" value="NAPRTase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007229">
        <property type="entry name" value="Nic_PRibTrfase-Fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098:SF1">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04095">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17767">
        <property type="entry name" value="NAPRTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000484">
        <property type="entry name" value="NAPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nicotinate phosphoribosyltransferase N-terminal" evidence="10">
        <location>
            <begin position="16"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Nicotinate/nicotinamide phosphoribosyltransferase" evidence="9">
        <location>
            <begin position="181"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Nicotinate/nicotinamide phosphoribosyltransferase" evidence="9">
        <location>
            <begin position="341"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="303"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="307"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4373900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="492" mass="54744" checksum="9AF8D6EFE015EB47" modified="2023-11-08" version="1">MALGSANLPEGIFSLLDTDLYKLTMQCCVLKFFPDVSVTYSFTNRTPDKKLSRAAFRWLQAQVDKLEHIQVSADELKFLKNTCPYLNDQYLHFLSTFRLKPSKQLKLSFNPTNDTGSDDDRGDFYIHTEGLWLDTILYEIPLLALVSEAYFKFVETDWSHAGQVDKAYQKGRSLLENGCIFSEFGSRRRRDYHTHDLVLQGLRRAADEGTKSGWKGKLTGTSNVHFAMKHDLMPIGTVAHEWFMGVAAITNQYENANEVALEYWTATFGEGVLSIALTDTFGTPSFLKAFKKQIPRVTTAVPGGGTTLASTPATTDPNSVQTMASTNPPIRAPYEEESGPRKTYAQVFTGVRQDSGDPLDFIKMMREFYDQEGIEDKKVIVFSDSLNLDLCYKYKAAAEAQGFQPTFGVGTFLTNDFVEESTGNKSVPLNIVIKIASASGRHAVKISDNIGKNTGDKATVEEVKKRLGYTEKTWAGGDEKTRWGTSGQPATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEY7</accession>
    <name>A0A9W8YEY7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_002284</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEY7"/>
    <dbReference type="OrthoDB" id="4685598at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.10:FF:000008">
        <property type="entry name" value="C6 zinc finger domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053181">
        <property type="entry name" value="EcdB-like_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47785:SF2">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47785">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="137"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="65"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4375198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="649" mass="70570" checksum="CD6418A7F6B6DEA6" modified="2023-11-08" version="1">MAAITPSPDVSPVSAATTNGRFPRDSFCLNLSTLPDANHFVLKSALRSPHLLDSPNGLFAGSGGLSSSFNFKKRPRNMSQSSGTDREMSSGTPPAPTNGMAPTSNGTHASSSEPRPTAKKRPSTDTIDYPRRRATIACEICRSRKSRCDGTRPKCRLCTELNAECIYREPGIKLDAGDKLILEHLNRIEGLLQTGLTNGFSLSSHSPAASNSTSEDFLARSTGNLSHLGMVQPINGIGTWTNHPSNISTMPKTHNTAALNLLSSPMIRELVSRPYDPKILLQLEMSRDSVSLGTSRGLDLSNTGAYVQAYFERVNVFYACVNPYTWSGHYQTALSHGFRDGPESCIVLLVLALGHAGSTGSITQQNPEKDPPGLAYFTAAWSLLPSVMTRNNMVAAQCQILAAAYLVYLVRPVEAWNVLSGASMKLQLLLSAPGRVSPAEEELGKRVYWNAVMIESDLLAELDLPHSGIAQFEEGFSLPTGFEDLGTEAVGRDELWYFLAEIALRRLLNRVSSMLYIRTTPYSKGPAATSIAQLDPPCGRAGLPTEPMACVRQLEYITAHHAGHLPYLFQGALSIISQTLLIMGATMNPSLSQLLPPPATMDDIINNVVLEMERYAHLAPSLRLSAEIIREAEGKRQMWLRTAGLKFEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF36</accession>
    <name>A0A9W8YF36_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphatidylinositol 3,5-bisphosphate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">HSV2</name>
        <name evidence="5" type="ORF">N0V83_001819</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF36"/>
    <dbReference type="OrthoDB" id="1667587at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4374743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="394" mass="42633" checksum="733A1AB312B1EBE8" modified="2023-11-08" version="1">MNTRPILDSDAGPFVLSASFNAENSSFSVALESGFRVFSSTACDLRIAREVGGGIGCAEMLGSSQYIALVGGGKQPKFPQNKVCPEDSDWHLINDTDIIYEVQIWNDKTQRVATSLEFNTPVQRVRISHTHLVVVLLNKVGIYKTKMPPVKVAEYETVNNPFGLCQLGENIVAFPGRTVGQVKLFDLTTSNVSIIPAHESPLRALAISKDGDLVATASEQGTIVRLWSFPSCTKLAELRRGIDPAAIFSLAFSPSGSTLAVTSDKSTLHIFDLTAAIQGISSQADPKQHKWGILSKVPLLPRQFSDTYSIASAKFELGDEPVVWGPHGKSATLSAGIPGVPGGRPTKGLLGWLDETTILIIGAGQDARWDRFVVGVDDTGRPAFVDHQWKRYID</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFA1</accession>
    <name>A0A9W8YFA1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 17">Pyruvate kinase</fullName>
            <ecNumber evidence="6 17">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">PYK1</name>
        <name evidence="20" type="ORF">N0V83_002626</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the pyruvate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="6 17"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFA1"/>
    <dbReference type="OrthoDB" id="108365at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00288">
        <property type="entry name" value="Pyruvate_Kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.33.10:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.20:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004491">
        <property type="entry name" value="PRK05826.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004978">
        <property type="entry name" value="PRK06354.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14 17" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="11 17 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15 20" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 17 20" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="18">
        <location>
            <begin position="32"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="19">
        <location>
            <begin position="393"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ4373887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="527" mass="57921" checksum="59E420EAA760E3D6" modified="2023-11-08" version="1">MANALDHLNAGRTRIEWLSQLNTEYHPAKEYRRTSIIGTIGPKTNSSEKINALRKAGLNVVRMNFSHGSYEYHQSVIDHSREAERSQPGRPLAIALDTKGPEIRTGNTVGDADIPIKAGTILNITTDDQYATASDDKNMFVDYKNITKVIEAGRTIYVDDGVLSFEVLEIVDDKTLKCKCVNNGKISSKKGVNLPKTDIDLPPLSEKDKADLKFGVKNKVDMVFASFIRRGSDITAIREVLGEEGKDIQIIAKVENQQGVNNFDEILKETDGVMVARGDLGIEIPPSQVFIAQKMMITKCNIAGKPVICATQMLESMTYNPRPTRAEVSDVGNAVLDGADCVMLSGETAKGDYPVEAVTMMHETCLLAEVAIPYVNAFDELRKLAPVPCPTTETCAMAAVSASLEQNAGAILVLTTSGTTARLVSKYRPVCPIIMVTRNSMAARYSHLYRGVYPFYFPEEKPDFNTEPWQEDVDRRLKWGIMNAIKLGVLNKGDPVICVQGWRGGMGHTNTLRIVPATEDLGLDMDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFS3</accession>
    <name>A0A9W8YFS3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001274</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFS3"/>
    <dbReference type="OrthoDB" id="5325862at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4375994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="264" mass="27910" checksum="10CFA4E158F004E6" modified="2023-11-08" version="1">MSGSTIAEDEKPPIDINTLTVEEKKDLVSADTISVLSSTTLDASSPTFTPVKSLHINAKGTPVLCLPVPPAELITAIHNPDGSLAYTSTRAKRSSGSCVLTDAEGTALIGTEYFFGPGKDPKLIRLDDVPEGTTAADSQEIKTASNWTSRNHRFLLPDGHTFAWSYKKEKGFGANGFKGTALVLTLGEKRVALLIRNDETRTPGSKSCSAGNGGELVLGSDVGGEVGIDEDLVVATVLLMLKKEVDRRRTVQFMMIASAVSGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFX9</accession>
    <name>A0A9W8YFX9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_000010</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFX9"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04077">
        <property type="entry name" value="Peptidases_S8_PCSK9_ProteinaseK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000014">
        <property type="entry name" value="Proteinase K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034193">
        <property type="entry name" value="PCSK9_ProteinaseK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF58">
        <property type="entry name" value="ALKALINE PROTEASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040943531" evidence="8">
        <location>
            <begin position="19"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="10">
        <location>
            <begin position="28"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="9">
        <location>
            <begin position="150"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4377187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="395" mass="42443" checksum="702769651D35C49E" modified="2023-11-08" version="1">MFTYILWAVTVLIAVVRSTPTSVPSGRYLIGFNEGVNVAEHVQWARDLHERNFVKRFDRKPRSDGVETVFRRINAYVGHFDVETIEELKTSPDVTYVELDQQFELFDASSSNFVTQQGAPWGLGLISHRDLNTTDYIYDSSAGEGHYAYVLDSGINVEHVEFGGRASLGYNAVGSPEDHIDDTGHGTHVAGTIGSTTYGVAKRCNLISVKITNVTGLYFSALIDGLDWALDDMITNNRLEKSVVNISGGGPTSTAVDNAVRQAYQSGMTIVAAAGNGNRSASYVSPAGALGVIAVASVDRSKKRAVLSNYGGTIDIFAPGEDTLSTWIGNSTATHVTNGTSMASPHVAGTVLYLQKLEGLTTPDQVLERLVELSVKDVVQDAQGSPNRLLYTGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGX6</accession>
    <name>A0A9W8YGX6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_001314</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGX6"/>
    <dbReference type="OrthoDB" id="200954at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16950">
        <property type="entry name" value="ZINC TRANSPORTER SLC39A7 HISTIDINE-RICH MEMBRANE PROTEIN KE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16950:SF16">
        <property type="entry name" value="ZINC TRANSPORTER ZIP13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040880146" evidence="7">
        <location>
            <begin position="21"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="360"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4376034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="411" mass="42839" checksum="7FDC94D7D219AA80" modified="2023-11-08" version="1">MVSRRNRTLLSLSIFIGVLAWNVAAAAVKGETGPAVGSLSAHEIEEQLQECPLVQALNEHKVASNPTASSLTAQIFAFLFPGSPAVNALLATLYISGPPNFLLALCPPNIDPSSLSVMVAFAVGGLLGDTLFHLLPEIFLGEDEHDKVKFVMVEPNKNLLLGVAIMVGFVTFVAMDKGLRIATGGEGGHDHSHGHSHDKAGVAVKATGAEVNDSKDALRARKPAANGITPPTTPLPTESDKEISASVKLGGYLNLIADFTHNITDGLALSSSFYASPTIGATTTVAVFFHEIPHEVGDFALLIQSGFSKRAAMGAQFVTAVGAFIGTCIGIAVQEFGGNSASADFQGPGLLGTSLQWGDMLLPFTAGTFLYVGTVAVIPELLETGPNKGQELRKTLTQFAAMFTGAGIMLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH19</accession>
    <name>A0A9W8YH19_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000602</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH19"/>
    <dbReference type="OrthoDB" id="5354320at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF25">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_3G11560)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="159"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="490" mass="54006" checksum="30F9848F4F3528C7" modified="2023-11-08" version="1">MESSPSAMLKLLLPKTPFILKTALWHTLGLAPTSSKWDLRTEITIGVLREMLGPNSPTSTISKVQRLTTKDPGVKGKVWASKVKLEVPEEDDVRQLLFKAINDMGTGEEQWTKPDSRPLEAEWNGYRADARDDEPEPAGLSEKEKYESLMKETSSKVTILYFHGGAMYLLDPATYRPTTSRIAKETGGRVFSVRYRLSPQNPFPAALLDCFTAYLSLLYPTPDAPHAPIPANEIVFGGDSAGGTCCTALLQLLLQIHRSTPTGETPTVKFHGKDVAIPLPAGVALTSPWLDISRSLPSIEAAAAKYDYLPPPSQTEKREFPPCEAWPTSPPRADLYCEGSALLHPLVSPLAAEDWSASPPLFFSVGEEMLSDEDAVMARRAAAQGVTVVWREFEAMPHCFAMLLEGLKAGHVHYEEFGKFCREVVDGTVAGTNGEFIVAKSLERREVDVKGGLTQLTDEEVRAYMEKGKERVERKMRRGEDASAEARPML</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH81</accession>
    <name>A0A9W8YH81_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UDP-galactopyranose mutase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH81"/>
    <dbReference type="OrthoDB" id="38045at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000220">
        <property type="entry name" value="UDP-galactopyranose mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43734:SF4">
        <property type="entry name" value="AMINE OXIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43734">
        <property type="entry name" value="PHYTOENE DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4375811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="541" mass="60317" checksum="4DDF9365CBFDABA7" modified="2023-11-08" version="1">MLNYARQTLKRVPSFQELLQGKMGSTKEEISVDVLVIGAGPTGLGAAKRLNQIDGPSWLIVDSNETPGGLASTDVTPEGFLYDVGGHVIFSHYKYFDDCIDEALPSDDDWYTHQRISYVRCKGQWIPYPFQNNLSALNKDDQAICMDGLIDAALEARVANTKPKNFDEWIMRQMGVGIADLFMRPYNFKVWAMPTTKMQCQWLGERVAAPNVKGAMSNIIHNKTAGNWGPNATFRFPARDGTGGIWIAVAKTLPESKTKFGEHATVTKVDAPGHKVFLKDGTVVNYKKLVNTMAVDALVETMGDKELVDLSKGLFYSSTHVIGVGIRGERPERIGDKCWLYFPEHDCPFYRATIFSNYSPFNQPQKDVKLATQQLADGSKPSSTEPKDGPYWSIMLEVSESSLKPVDGKNMLKDSIQGLINTEMIKPGDEIVSTYHRRFDHGYPTPSLEREGVLTKLLPALQAQDIWSRGRFGSWRYEVGNQDHSFMLGVEAVDNIVNGAVELTLNHPDFVNGRKNEERRLQDGAQIFAKAPLALKNKGID</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHA5</accession>
    <name>A0A9W8YHA5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PEBP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000829</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHA5"/>
    <dbReference type="OrthoDB" id="2506647at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030414">
        <property type="term" value="F:peptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030162">
        <property type="term" value="P:regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046578">
        <property type="term" value="P:regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF85">
        <property type="entry name" value="INHIBITOR (TFS1), PUTATIVE (AFU_ORTHOLOGUE AFUA_4G08120)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="22589" checksum="68201CCF07DAE013" modified="2023-11-08" version="1">MASTTLLDSLRTANLLPSKVIPETSFTPIFSLTITFPSISPSNGNFVRVSEVKQQPVISITSSSPSSPSSHSAQSFTFMLIDPDAPTPDDPKFGYWRHWVVTNIPFDSKSSAGEDADIIQRGRTLTPYLAPGPKDESGPHRYLFLLFKEPEGLKLEKSDVGGEEFVDRRSFRAEEFVKTHGLELVGVQWMRGVGDGWKEGKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHA9</accession>
    <name>A0A9W8YHA9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">DNA damage-binding protein CMR1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_002343</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">DNA-binding protein that binds to both single- and double-stranded DNA. Binds preferentially to UV-damaged DNA. May be involved in DNA-metabolic processes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the WD repeat DDB2/WDR76 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHA9"/>
    <dbReference type="OrthoDB" id="9890280at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000001">
        <property type="term" value="P:regulation of DNA damage checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000562">
        <property type="entry name" value="DNA damage-binding protein CMR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050853">
        <property type="entry name" value="WD_repeat_DNA-damage-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14773">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14773:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="352"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="32"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4375257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="523" mass="57659" checksum="66A3BA967EEA6651" modified="2023-11-08" version="1">MARDKNAELSEYERKRQENIAKTQALLRNLEMEAAQAGLGPTGKAKAPASSKPKSKKPAAKKIKQEEIAPRRTSSRLKGIEADSEKAKRKAEEEFVAIKEADRAKRQRVSGAFNFSDIVVAGKDWNQSGNFLSIVGPANPYERTFDFDDAKETTDQELRALREKMSGLQLWEDFEPNEIKITPERIYAMGLHPTTEKPLVFAGDKLGNLGICDASQKAAEVKQEDDEDADAEGPTITTLKPHTRTIHTFQFSPHDSNALFSASYDSTVRKLDLAKGVAVEVYAPSDVNEDAPLSGIEISKDDPNTLYFSTLDGRFGIYDMRTSSEKAAGLEIFQLSEKKIGGFSLHPLHPHLVATASLDRTLKIWDLRKMSGKGENRLPALVGEHESRLSVSHAAWNSAGQVATASYDDTIKIHDFGNCADWKVGSSLTDADMKPSIVVPHNNQTGRWVTILRAQWQQFPQDGVQRFCIGNMNRFVDIYTAKGQQLAQLGGEGITAVPAVAKFHPTLDWVAAGTASGKLCLWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHI6</accession>
    <name>A0A9W8YHI6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHI6"/>
    <dbReference type="OrthoDB" id="25921at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046394">
        <property type="term" value="P:carboxylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050571">
        <property type="entry name" value="Class-IV_PLP-Dep_Aminotrnsfr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743">
        <property type="entry name" value="AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743:SF11">
        <property type="entry name" value="AMINODEOXYCHORISMATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="329" mass="36775" checksum="17FB352E998EED9A" modified="2023-11-08" version="1">MATMNQVFAGYHSRQAVLEATFDKNPFARGIAWVEGEFVPVNEARIPLLDQGFMHSDLTYDVPSVWDGRFFRLHDHLDRLQASCKKMRLNFPIPREQIEKILVEMVAKSGIRDAFVELIVTRGLKGVRGHTLGETFKNSLYMFVTPYVWVMEPEVQPVGGKAIVARTVKRISPGSFDPTIKNLQWGDLTRGLWEALDRGAVYPFLTDGDANLTEGSGFNIVLIKDSVLYTPDRGVLEGVTRKSVFEVAKSVGVEVRVEFVPTELAYNADEIFMCTTAGGIMPISELDGKPVKDGKVGPITKKIWDGYMELKYSPTHSFEIDYSSGTAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHR6</accession>
    <name>A0A9W8YHR6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">P-type Na(+) transporter</fullName>
            <ecNumber evidence="20">7.2.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">N0V83_000386</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="26">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>K(+)(in) + ATP + H2O = K(+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:75815"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>Na(+)(in) + ATP + H2O = Na(+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:14634"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IID subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.3" evidence="20"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHR6"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008554">
        <property type="term" value="F:P-type sodium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000193">
        <property type="entry name" value="Plasma membrane calcium-transporting ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000160">
        <property type="entry name" value="Sarcoplasmic/endoplasmic reticulum calcium ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000015">
        <property type="entry name" value="Sodium ion P-type ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006414">
        <property type="entry name" value="P-type_ATPase_IID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01523">
        <property type="entry name" value="ATPase-IID_K-Na"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0915">Sodium</keyword>
    <keyword evidence="18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="13" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="346"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="813"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="846"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="896"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="937"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="993"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="1026"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="25">
        <location>
            <begin position="52"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="467"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="475"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00035017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00035029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00049499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAJ4377561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1082" mass="117837" checksum="7235C3A5C445793C" modified="2023-11-08" version="1">MGETVLPTVEETSPQAEEPPICGLNGPKSTHHQQRQFQPNPDDGDTPLALHAPHTLSFEEVAEKLFVDINHGLTYAEAESRLRLYGPNKVKGAEGLSMWKILLRQVSNSLTFVLIIVMAISFAIGDFIEAGVVTAVICLNIVVGFWQDYRAEKTIESLKKLTAPEATVTRNGTPDIKVKAVDLVPGDIVQLSVGSIVPADLRLIDSVNAFTNEAFLTGEAIPVEKTPLEKFDDDELATGDRTNLAFSGSEMTSGRCRGIVVATAMHTEVGKIATMLRQKNDDGAQSQNIIVRLSKRFVNGLKTILGLVGTPLQITLSKFALLLFGLAILLAIIVFSASKWQVGGEVLIYGICVAVAVIPESLIAVLTITIAVGGKAMAKAHVITRVQSAIEAVGGVTNICSDKTGTLTQGRMVTKKAIIPGFGILTVDDIADPFNPGSGNVYLDGTYVTLDDYTPDTVPSYNRSMQQLQGLPTPEHSDRAWSSEKKDALDPSISEDDRDTDGSTIQSLSRPPTAIMQGLSSGPWKAHGEPTEIALEVLASRFNMGKQNLLRTQNIDLATEFSFDSTVKKMTVVYKHDHTGHMDVYSKGATEFMLPVLNVTDQEKDGILQAAENMANDGLRVLCIAHKSLQPGTDIHERASVENNLNYIGLVAIYDPPRSETKGAVQECKIAGITVHMLTGDHPGTARAIAREVDIIDDRTPPSAVMIAREFDQMTEEQVDALQTLPLVIARCSPKTKVKMVQALHRRGRFCVMTGDGVNDSPALKQANVGMAMGSGSDVAKDAAEMVLTDDNFASIVKAVEEGRRLFDNIQKFLMHLLISNIAQVILLLIGLAFKDNDNHSIFPLSPIEILWVNLITSSFLAIGLGLEESQEDSMFRPPHDVKKGVFTTELITDKFIYGFFMGALCLFSFAIVAYVGPGGGNLGSGCNEGYNDTCDVVFRARATTYATITLLLLVTAWEVKHFSRSLFNMYPNTDQGVPRGLSIFPTLWRNRFLFFAVIAGLVLMFPIVYIPVINEKVFKHASITWEWGVSFGAVVIYISAIETWKAIKRSFRIWSGAHRVLSREDAEARAGLAGGLVVQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHU0</accession>
    <name>A0A9W8YHU0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase 2A activator</fullName>
            <ecNumber evidence="8">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Phosphotyrosyl phosphatase activator</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RRD2</name>
        <name evidence="10" type="ORF">N0V83_001407</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for PP2A-like phosphatases modulating their activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2Ai) in presence of ATP and Mg(2+) by dissociating the inactive form from the complex.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the PTPA-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHU0"/>
    <dbReference type="OrthoDB" id="16120at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008160">
        <property type="term" value="F:protein tyrosine phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04087">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1150:FF:000002">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004327">
        <property type="entry name" value="Phstyr_phstse_ac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043170">
        <property type="entry name" value="PTPA_C_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037218">
        <property type="entry name" value="PTPA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012:SF5">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A ACTIVATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03095">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016325">
        <property type="entry name" value="Phstyr_phstse_ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140984">
        <property type="entry name" value="PTPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0697">Rotamase</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4376126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="415" mass="46599" checksum="41D8AD23C9196A1A" modified="2023-11-08" version="1">MTQPQSVPPAASSPPLDLSDKLPKLVPRKRAPQPANRPPPYLEKHEYLRPSRRILSPEDHQLFLKSETCTLVEAFIYGIADSVRDRSVSSIKDAEVTDTVKATVQILGEAEKVLERCPPEDTGSRFGNPVFRTFLDEVESALPTWHTTLGLTDQAQVDEISTYLHNSFGNKRRIDYGSGHELNFFLWLLCLNRLGLLPRTAFPALALVVLPAYLRLMRKIQESYYLEPAGSHGVWGLDDYQFLPFLFGAAQLLHHPYITPKSIHNALVLEEEGKEYLYLDQIAFVNSVKNVEGLRWHSPMLDDISAAKNWGKIEGGMRKMFRKEILEKLPVMQHFLFGSLIPAVEGMSTEEEAGVEHEEEESEGGEVKVFDDEAGKRHVHASAGWGIAVGYECQLRLGRRAKRGKGCEAIKEGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIP4</accession>
    <name>A0A9W8YIP4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable enoyl-CoA hydratase, mitochondrial</fullName>
            <ecNumber evidence="3">4.2.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_000902</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Mycotoxin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIP4"/>
    <dbReference type="OrthoDB" id="2018133at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000019">
        <property type="entry name" value="Enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000001">
        <property type="entry name" value="Probable enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF54">
        <property type="entry name" value="ENOYL-COA HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0843">Virulence</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4378071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="289" mass="31125" checksum="DC6A26823D48B0DA" modified="2023-11-08" version="1">MQAFRSTVRVASQFRPTGLPRLVRGYATEKTYEHLLVSTPKPGVGLVQLNRPKALNALFTPLILELNQALRGFQADQSIGAIVLTGSDRAFAAGADIKEMKDLTFSDAYGQDFIETWSDLVTFLKKPLITAVNGYALGGGCELAMMGDIIYAGPKATFGQPEIKLGVIPGAGGSQRLTKAIGKSRAMETILTGDNISAKQAGEWGLVAKMFETPEECVNGALATAEKIASYSQIAVKACKEVVNKSQDLGIREGVEYERRVFHGLFGSQDQKIGMAAFAAKEKAKWSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CG72</accession>
    <name>A0A9W9CG72_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010739</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CG72"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="408" mass="44418" checksum="909F46D1108EDAD8" modified="2023-11-08" version="1">MELATFEEAQRRRDQARLDAVKQAAPQDDGGELERRVAATLAMPAPQGDSVAGAREFLAAYRTRPVETAGQEATEVDLTNRSSKRIRLLDYDRDYSHTPYPPIGLDTGEMQRSGPRGEQHPSQSSARGGYMAMDMGGYQPQGAVAGASNRDQAQVAVTGLQRFPTRDAVRDLYNLQETEEHSDVRLLGGARNRYPPQVAVPGYRGLRHLSRVYVQSDDTESGRIDGHTRDLDVPSLGPQIQRGRSSQASAGGNTTPLPSTDSGFLEQESGRADDRGMPVALRVGGEWVRGAAVPSTVKQRLVEELLLPIQSNKDRMQRWDSMGVMAKVCILTRVIGKQTTGSFKDANEAKEACATCARMGGKAGRKADPRPCAVLLSDDGTTTIGFLPLPAGLRPGKAWEEVGYWIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHI5</accession>
    <name>A0A9W9CHI5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">hydroxyacylglutathione hydrolase</fullName>
            <ecNumber evidence="5">3.1.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glyoxalase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GLO2</name>
        <name evidence="12" type="ORF">N0V83_010628</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an S-(2-hydroxyacyl)glutathione + H2O = a 2-hydroxy carboxylate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21864"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58896"/>
            <dbReference type="ChEBI" id="CHEBI:71261"/>
            <dbReference type="EC" id="3.1.2.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.6" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHI5"/>
    <dbReference type="OrthoDB" id="515692at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07723">
        <property type="entry name" value="hydroxyacylglutathione_hydrolase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035680">
        <property type="entry name" value="Clx_II_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032282">
        <property type="entry name" value="HAGH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935">
        <property type="entry name" value="BETA LACTAMASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935:SF94">
        <property type="entry name" value="TENZING NORGAY, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16123">
        <property type="entry name" value="HAGH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="10">
        <location>
            <begin position="61"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Hydroxyacylglutathione hydrolase C-terminal" evidence="11">
        <location>
            <begin position="129"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4362534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="207" mass="22960" checksum="BA18B7ABCD33BC96" modified="2023-11-08" version="1">MPILFQTTKRKKHLSLTRPTRQNTPQAKEFPVPIVGGKDCALVSSTPEHKYSFAFGSIKVTALHTPCHTQDSICYFLEDGSDRAVFTGDTLFIGGCGRFFEGTPEQMHTALNETLSALPDDTKVYPGHEYTKGNVKFAKSVLPDDAAVKRLDEYSQANKETQGKFTIGDEKQHNVFMRVTDPAIQKITGMTKPIDVMGALRSMKDKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHJ5</accession>
    <name>A0A9W9CHJ5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Integral membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010494</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHJ5"/>
    <dbReference type="OrthoDB" id="860at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF93">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN 25D9-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="230"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="233"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="246"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4362401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="28375" checksum="2899BCAE43DBBF57" modified="2023-11-08" version="1">MSVKFEKETVKTTTASVADAASKGKDDLLHEVGQALTKGGGPNGYLAAYLKQLQSNPLRTKMLTSGTLSGLQEFLASWIAHDRSKSGHYFTSRVPKMALYGALISAPLGHVLISLLQKIFQGRKSLKAKIMQILVSNLVISPIQNSVYLVSMAIIAGARQYHQVKATVKAGFWPVMKVSWVVSPISLAFAQQFLPESTWVPFFNIVGFIIGTYINAHTKKKRLAALRRKHYGDGSGRSQGPVGGRPGDEYGRPDDRPRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHJ6</accession>
    <name>A0A9W9CHJ6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH2 dehydrogenase 14K chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010658</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHJ6"/>
    <dbReference type="OrthoDB" id="2141050at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39218">
        <property type="entry name" value="OXIDOREDUCTASE 14 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12110)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39218:SF1">
        <property type="entry name" value="OXIDOREDUCTASE 14 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12110)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="86" mass="10069" checksum="4D0EC32E0184D47C" modified="2023-11-08" version="1">MVHKVLFWSGLGIAARLWQLGIEMRPLFNKESLWVYPVYAGIGGSFGYWLMGVEQRQFRLLADRRDALLEKRARRKEREDAAREAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHY8</accession>
    <name>A0A9W9CHY8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alcohol dehydrogenase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_009681</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHY8"/>
    <dbReference type="OrthoDB" id="1560166at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000003">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43350:SF2">
        <property type="entry name" value="GROES-LIKE ZINC-BINDING ALCOHOL DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43350">
        <property type="entry name" value="NAD-DEPENDENT ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="6">
        <location>
            <begin position="147"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4363388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="266" mass="27692" checksum="D8AC5F49398E923D" modified="2023-11-08" version="1">MVGAEVKGVRVGDRVVLSFDYCGGAEGCRACDSSVKGMGMPGYCKEFHARNIFGVEGVYQVVVDDDVDDGEEGSKAAAGLFFGQSSFSQFAVVKGRSAVNVQAVVQSEEELKLFAPMGCGYQTGAASVTEVADVREGDSVAIFGLGGVGMAAVMAAKIRGAKTIIGVDKIQSRLDLAKDLGATHVLDTSNFPSLTSDLTAAIREIAPSGTNATFDTTGVIPLIEVGVRALHANGQMILIGTVNGSMMLDLGMLLNVREVSLRSNIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI95</accession>
    <name>A0A9W9CI95_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alpha-1,2-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI95"/>
    <dbReference type="OrthoDB" id="5043642at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021848">
        <property type="entry name" value="HODM_asu-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11927">
        <property type="entry name" value="HODM_asu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="490" mass="56882" checksum="4775B0BEE5F9E4E6" modified="2023-11-08" version="1">MASNILLLLGAAFIAGSAYLILQRGKRNIILEQLRVSRRRVSGAKTPPRSLSPGKKQLQAPEANYADTFPPSRRFTLADTDLPARLSKAPEKLISSQPDWTKNMLPIELSYLDSNESMYTPCEFSVKEIRTLGNFPDYATLSGVPLPSPYPEFDIRKALPRPYRPFRWAYHQTMSLTKLEPDWWLELENTYEQRIKQRQDLYADHGKAVLQALPGSELACKELMEMSLQFLCARYPHYFTLDKENMIFHNSILKTETDLNTTHPLHVLLNNVPEDFAIMLRDDQTGTYLFRAGIICSALGWNVGTKIGMRLHEIHAPIPDYKEKMQFSMDRYFAKKPTDKSIQRGSWGLEVDQPLYMPPGHPDEKHREYQMADLDLSRCHLRVDWQTLRRLPLSAGVVFNFKALFTPIEDFRREPYIPGLVLKVLNDGKKSLMEYKNTWHVEHVVKPALEKFHKEQVERGLVPEDWEPHTLEESPWFPGWEQKWHSQQGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIA9</accession>
    <name>A0A9W9CIA9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_009970</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIA9"/>
    <dbReference type="OrthoDB" id="203279at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009332">
        <property type="entry name" value="Med22"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06179">
        <property type="entry name" value="Med22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4363674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="151" mass="16337" checksum="F41539F773CD56A2" modified="2023-11-08" version="1">MPPRLLAKTEQLCNSIADNRNADPWGGDGYLSTILRTIKMEESSDYAPAGQTRLNGAYDYATSAYKELVIKEQAAGLIKTAQDISTLIRDLQELWLFGGLDTLSDPADEEASRVKAIAVAEMIEVLAKKELVKGEDKGTGDGVMKVEEGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ53</accession>
    <name>A0A9W9CJ53_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008691</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ53"/>
    <dbReference type="OrthoDB" id="5377012at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="389" mass="40908" checksum="E9D55E70FBBA7285" modified="2023-11-08" version="1">MLIRASSDRQSRKRPFAGWMKRLANLKPSSSESPSKTTTSTKARKQAAKNNPYPESGYNNTANAPQHLSFSTPGTPRSNDSYTSVEESTSGQRQALAKSNKSTAPTVATVPETVHSARSKAETGGSNFVNGGSTFSSPHGSEHSLTTTLTTIQSTAPSNVLNGAPSNAPAPGTTPVHFSHQFPTSPPPSAIPPHLAPQQQQPNSYQGATANNILTDNASILTLASSSKRRRRNSLDTNASVRALAPSSHYGGSRESLPLSVLSGNADTIYSPSTRPSNVGGFANAERASVYSASGVTAPVLPSERNSYYANKQNADGLSIRSGLLGHGRTDSISSIRATPTSPLASPRDPVGPGRIRQSAEWKDDREASDEDEAPSNPPVAEHEDKAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ80</accession>
    <name>A0A9W9CJ80_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 18">CDP-diacylglycerol--serine O-phosphatidyltransferase</fullName>
            <ecNumber evidence="5 18">2.7.8.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 18">Phosphatidylserine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">CHO1</name>
        <name evidence="22" type="ORF">N0V83_008189</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>a CDP-1,2-diacyl-sn-glycerol + L-serine = a 1,2-diacyl-sn-glycero-3-phospho-L-serine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16913"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="17 18">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18 19">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.8" evidence="5 18"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ80"/>
    <dbReference type="OrthoDB" id="448573at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003882">
        <property type="term" value="F:CDP-diacylglycerol-serine O-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006659">
        <property type="term" value="P:phosphatidylserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000022">
        <property type="entry name" value="CDP-diacylglycerol--serine O-phosphatidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050324">
        <property type="entry name" value="CDP-alcohol_PTase-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004533">
        <property type="entry name" value="CDP-diaglyc--ser_O-PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016271">
        <property type="entry name" value="CDP-diaglyc--ser_O-PTrfase_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00473">
        <property type="entry name" value="pssA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269:SF61">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--SERINE O-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000852">
        <property type="entry name" value="Phosphatidylserine_synth_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 18" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 18 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 18" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="15 18" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="80"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR000852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ4365569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="242" mass="26857" checksum="27846F4E54E294CF" modified="2023-11-08" version="1">MAQRRNGAPQGPKDEAPPPKDLTQDKQARLLSQEAGHFSLVRALHLADFITELNGFCGIMSVMSSLRYCIQDDPHNYTNLYFALAFIPLGLFFDFMDGKVARWRKKASLMGQELDSLADLVSFGVSPAAAAFCLGLRTPVDHLLLSFFVLCGLTRLARFNVTVAMLPKDASGKSQYFEGTPIPMSLILVQVMAYWVYKGWVLEQVPMGLWLEGTVAEFHPVVAMFVLHGCLMVSKSLKIPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJG9</accession>
    <name>A0A9W9CJG9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Translation initiation factor IF-2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">IFM1</name>
        <name evidence="14" type="ORF">N0V83_008476</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJG9"/>
    <dbReference type="OrthoDB" id="361630at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03702">
        <property type="entry name" value="IF2_mtIF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03692">
        <property type="entry name" value="mtIF2_IVc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000008">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000001">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000019">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_B">
        <property type="entry name" value="IF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053905">
        <property type="entry name" value="EF-G-like_DII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044145">
        <property type="entry name" value="IF2_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006847">
        <property type="entry name" value="IF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000178">
        <property type="entry name" value="TF_IF2_bacterial-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00487">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF20">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22042">
        <property type="entry name" value="EF-G_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04760">
        <property type="entry name" value="IF2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01176">
        <property type="entry name" value="IF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 14" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tr-type G" evidence="13">
        <location>
            <begin position="484"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="30"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="256"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="764"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="132"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="306"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="319"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4365854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1012" mass="111692" checksum="FE11F23D76EFAB85" modified="2023-11-08" version="1">MRRAHGFRVSSTRDVCVFCATRQLALAHTSSVSTQRRQLNSSSRAPQSAEGIIYKDANPAPKPMSQAQRMRQALAASAQFTPPAQPQGGYMPPGQRNKPSFGQQAPQAQRQPPPDRRPVNRGGVFDGPGRNQNRDRFAPIARRDGGTSRSSDLRASPQPRYKVNMDSRLQVNTPAFSAGHLRRQNEPQPGRAQIRVSEQSPAARPAPRRSILDQDEIDKLLGNTATTSSRPTKPFQPFARDANAQRKCWHCGSTDHMSNACSVKPTLREQPQQEQRKTMYRPPQSQRQEESVADAAEAWARKQKGFMQTGQTSQPQEDEDRRARNVEQDRRRNRFELDEPMEVKNRYEEPERRKGGNRGRRIQRDVEEDDDRPYMNKQERKRLRAEAKKAAQAAKNGPTPISLPEYISVANLAGALRLRVEDFVQKLEELGFEEVQNDHILNAEHAGLIAQEYNFEPIFQAEEDDGDLYPAPAVEPEVYAELPARPPVVTIMGHVDHGKTTILDYMRSTSVAASEFGGITQHIGAFSVPLANGKKITFLDTPGHSAFETMRARGANVTDIVVLVVAADDSVMPQTVEAIKHAQAAKVPMIVAINKIDKQQADPDAVKLDLGRHGIEIEDFGGDVQAICVSGKTGQGIPELEEAITTLSEMLDHRADPQAAVEGWVLEGTTKKSGRVATVLVRSGTLRQGTALVAGTTWTRVRSLRNEAGVVVKEVGPGMAVEVDGWRDQPIAGDEVLQANDEQQATAVSELRTEKVEREQMARDMEAINESRRLEAERREAEEAAEKAAANGEEAVVEEVKAAGPEVVSFIIKGDVSGSVEAVIDSVSALGNAEVSTRILRHGVGPPSEFDVSHAADAQGHIVNFNTSIPNHVAKLAEEKGVMILDSNIIYRVVENVRELLSEKLKPNVIQKVTGEAEIAAVFEIGLGGRKKMKVAGSKVRNGQIDRGTKARVLRGEVVVHDGVITSLKNQKKDVEQMRKDTECGIAFDEWEDFKVGDKIQCYEEKHEKRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJY5</accession>
    <name>A0A9W9CJY5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007817</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJY5"/>
    <dbReference type="OrthoDB" id="3801354at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="110"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="237"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="314" mass="33364" checksum="B7B4391FD8E8D2B3" modified="2023-11-08" version="1">MDFLKKKMKELMDDDDKPKPQGDSHGSSSGAPPGGASHGERSAANDYYGGSSQGGYPPQQQQYPPQGQGYPPQGQPQGYAPQGYQQQQQQPYGQGAATPVGPPAPYGAPPPMPPGWTQQWDQNSQRWFYIEQATGRSQWDPPSHLPPGPYAPPPSGAPYQPPGGHDERGLFGNTQGHQGHDYTPSGHATNEAEKKKDKGHSTAMLAAAGIGGVAAGAWIGHELTEDSDDEKHTQSYAAAPAAAPAAAPGYAPAPAPAADPYGDQAFAPPPPVPTHDEDGSSISSSDRESMEEKREELIEAQEEYQEEVEENYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKA3</accession>
    <name>A0A9W9CKA3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">CCAAT- binding transcription factor component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">HAP5</name>
        <name evidence="10" type="ORF">N0V83_007436</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NFYC/HAP5 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKA3"/>
    <dbReference type="OrthoDB" id="1272441at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22908">
        <property type="entry name" value="HFD_NFYC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000017">
        <property type="entry name" value="Ccaat-binding factor complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF8">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="9">
        <location>
            <begin position="87"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="264"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4366907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="300" mass="33230" checksum="A885CE1DECEA62BF" modified="2023-11-08" version="1">MDSNLQKMRQAPAAPRRYDGQAGNPLYDPQQGGHYGASASIAAQGHAPDPQLFTGSWQNVNQGLTGNYRDILNTYWQQQVTKLETDEHDYKLHQLPLARIKKVMKADPEVKMISAEAPILFAKGCDIFITELTMRAWIHAEENKRRTLQRSDIASALSKSDMFDFLIDIVPREEAHPHKRSAGQNAAVQSSAAVVPPGGMPQPVHAQHQMAPPDYGMGQQIPHEEFQQPTMYPGPVQGDPRAYGQQQMFDPGVYGAYPMAQQQMYPVGQRGPDPGAGDPQGYRHEEADADAEGEREDFGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLH8</accession>
    <name>A0A9W9CLH8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005688</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLH8"/>
    <dbReference type="OrthoDB" id="3660917at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="200" mass="22222" checksum="3B70FF2167238965" modified="2023-11-08" version="1">MSDTAPHAPEGLVHGTDRSQPPNQPQQKEPNEHTPKLPDANLSAGSGHTSQTSNLRDFLASLKAPADRKFFQALGKDGVLRNLCFLPTPPDQPTGITVYDAKPMSPELIKAYLDRQPWSQATEDRFRGVDGRLVAQEHWLQPPPGVVLSRGSQNERDEEKRRWAEERKLLEEKIERGEVSAEDAGPACGGVQSNYDLRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLM1</accession>
    <name>A0A9W9CLM1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1996 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005808</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4370044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLM1"/>
    <dbReference type="OrthoDB" id="74764at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018535">
        <property type="entry name" value="DUF1996"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43662">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43662:SF6">
        <property type="entry name" value="DUF1996 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09362">
        <property type="entry name" value="DUF1996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040963592" description="DUF1996 domain-containing protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1996" evidence="2">
        <location>
            <begin position="52"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="371" mass="40367" checksum="AF8A18B90133B28B" modified="2023-11-08" version="1">MFWGTLLALATLTEAAPSWDAPKWLTNKLSKRQGGTTMLRFGCAQVVIDRLDPLVNSGMAPSPHQHQIVGGNGFNVTMTTGDVSKTASCTTCAFSKDRSNYWTANMYFKSPKNGTYKRVKQLGAAKQFSDDFSTQIGGGVLVYYVSAQPGKITAFKPGFRMLVGDPTNRKADPKMKMQSCFRCYTGPNFGGDNAAPCADGKLDTQSMPTAPCKGGIRSNVLFPTCWDGTNLDSPDHKAHIAYPTTGPANFLSLGGSCPSTHPVRIPQLMYEVVWDTTPFNGEWKSGDPNPFYLSYGDNTGLGQHGDYVFGWQDDELQKGMDATNCMGAKCKDLKNQVIADAKACNVKKVVQDDLDAWHTEIPGQGSMPMQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLR5</accession>
    <name>A0A9W9CLR5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chitin biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_006204</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLR5"/>
    <dbReference type="OrthoDB" id="245697at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034044">
        <property type="term" value="C:exomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000747">
        <property type="term" value="P:conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17742">
        <property type="entry name" value="BRCT_CHS5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13945">
        <property type="entry name" value="Chs5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000088">
        <property type="entry name" value="Chitin biosynthesis protein (Chs5), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000453">
        <property type="entry name" value="Chitin biosynthesis protein CHS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.120.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031673">
        <property type="entry name" value="Chs5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052827">
        <property type="entry name" value="CHS_Export/Cell_Fusion_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031669">
        <property type="entry name" value="Fn3_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47351">
        <property type="entry name" value="CHITIN BIOSYNTHESIS PROTEIN CHS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47351:SF1">
        <property type="entry name" value="CHITIN BIOSYNTHESIS PROTEIN CHS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16892">
        <property type="entry name" value="CHS5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16893">
        <property type="entry name" value="fn3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12738">
        <property type="entry name" value="PTCB-BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="75"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="163"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4369120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="406" mass="44558" checksum="50545BCD456017F2" modified="2023-11-08" version="1">MLVSLTVGKVDAGVAVLLTEDKRLIEFPSILLPPDISSGSIVDIDVGRNYKAEADAAKSFHELQSDIFRTFGQRTPSAPAIRCRNATQTSVVLEWDPIDVATAELRSLTLYRNGSKAGTIPKPSDVHSTKLSGLAVDTEYTFHLVLKTSAGTFSSDKLKVQTHKMTDLSGITITPGHMPAALRESLQRSVERIGAKIADNVRIDTTHFVCTEGRGPAWEKAVENNIPVVVPDWVIGCEREGRIVGVRGYYLNADPRLRNVGPGASQQQPQHTQSQQQQQQQQQQVRSPIQSPRTEVTPPTPERPTHDRDTDSAPPPPPPKDSISEKSASTTPEPKSQPEEQKVRTEDLKPQSEKEQEHVEDSEEDESEPESHNEKALEAGLPNRPRPGQASVEDDDDGGSFQEVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM02</accession>
    <name>A0A9W9CM02_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_005343</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM02"/>
    <dbReference type="OrthoDB" id="1663137at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04733">
        <property type="entry name" value="OYE_like_2_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051799">
        <property type="entry name" value="NADH_flavin_oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43656">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G08260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43656:SF5">
        <property type="entry name" value="NADH:FLAVIN OXIDOREDUCTASE_NADH OXIDASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="5">
        <location>
            <begin position="18"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4369581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="449" mass="49351" checksum="3D4AD8F8E62F89C9" modified="2023-11-08" version="1">MAPPRYEAEHKDASPLGKPLDFAFSKKTAQNRFMKAAMTERLSSWDPKNLEARGVPSKNLINVYRRWGEGEFGVILTGNIMVEYDHLEAPGNPIIPRGAPYSGERFDAFKELATQSKKHGSLIVGQVSHPGRQVTENVQKHPISASDVHLDKNIMGMTFAKPRAATDEDIQNVIEGFAHSAEYLEKSGYDGIELHGAHGYLLAQFLAPSTNHRTDKYGGSLENRARLIIEIGNEVRRRTSSSFVLGIKLNSVEFQDKGFDTQEAATLCKLLEDNSFDFVELSGGTYENLAFEHKRESTKKREAFFLDFAEKITPGLSKTRSYVTGGLRTASAMVDALNAVDGVGLARPICLEPELPKEIIKGEVHAAIRQLIDDQDFGTTNVAAGTQIRQLGKDQQPIDLSDEKNVQAFQKDVGTWQKGLADDAEGSKYGYVDIESVQSVAYGGAAPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMP8</accession>
    <name>A0A9W9CMP8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Branched-chain amino acid aminotransferase II</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_005199</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMP8"/>
    <dbReference type="OrthoDB" id="409992at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004084">
        <property type="term" value="F:branched-chain-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009081">
        <property type="term" value="P:branched-chain amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000010">
        <property type="entry name" value="Branched-chain amino acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000004">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01123">
        <property type="entry name" value="ilvE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42825">
        <property type="entry name" value="AMINO ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42825:SF2">
        <property type="entry name" value="BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE 3, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006468">
        <property type="entry name" value="BCAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006468-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4370678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="373" mass="40757" checksum="377B369D92849226" modified="2023-11-08" version="1">MADFPPPPVDTIDWNNMGFKIREVAGHVESHYSRESGKWTPPVFVEDPFLRIHGMAPGLNYGMQCYEGLKAFRAPDNTINIFRPTKNAERMQHSASFISIPDVPTDHFLACCSLAVGMNAAYVPPHETGGAMYVRPLLFGSSAQLGLNPPQEYTFVVYCIPTGVYHGTNPVDALILESFDRAAPEGTGSAKVGGNYAPVLRHSEKAYKAGYGITLHLDSKTRSVIDEFSTSAFIGVKKDGDKIKLIASNSKNVIQSVTGESVLRIAESFGWETESREIPYDELKTFDEVLAAGTAAALVPIKSITMESKGDKFTYPEAAEVPGPVCVKLLTTLKGLQQGKIEDKQNWLFRVEKPKHFGKKETNGGAKTVGQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMQ4</accession>
    <name>A0A9W9CMQ4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_005140</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the INSIG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMQ4"/>
    <dbReference type="OrthoDB" id="205546at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025929">
        <property type="entry name" value="INSIG_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15301">
        <property type="entry name" value="INSULIN-INDUCED GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15301:SF3">
        <property type="entry name" value="PROTEIN NSG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07281">
        <property type="entry name" value="INSIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="230"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="326"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="113"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4370619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="382" mass="41963" checksum="701B53D4C23432B2" modified="2023-11-08" version="1">MAEESFMPSNAAQPPPHIYRPIPRRNFDSTSHDDSADSPTHAFSQSTPPRTNESRRSSDFLAQLNARLLRTYNSRNEDAEEQEREKGPPPRNKSFLNMTSSTLYGIYDGMSSGAAGERSTAETPWGTGAETPAHSGMGIPAWETGMGSPDAGLSMKNHARRRTGQGKAEAKGKSHATRHSRQGVWKYAVMFGKLSALYSFGVVYGLIVSHLHETRQLAAVHVDGVDRRSWIYLASWGLFGVTLGSLLPYVDLVWDGQKVEKEMERNDRDSPISEQINDVVRSVCAFVGIAFAIRRLPWQSTLQLTLTLALVNPALWYILDRSKPGLSLSLVITSILTSCIFLSNPDVLPSPSLPATTNVTQLPTTLGRSQTPTLEPKRNVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN17</accession>
    <name>A0A9W9CN17_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carboxylesterase type B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_004572</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36681">
        <property type="entry name" value="NUCLEAR GTPASE, GERMINAL CENTER-ASSOCIATED, TANDEM DUPLICATE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36681:SF3">
        <property type="entry name" value="NUCLEAR GTPASE, GERMINAL CENTER-ASSOCIATED, TANDEM DUPLICATE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="4">
        <location>
            <begin position="1283"/>
            <end position="1774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="342"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="604"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="682"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="767"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="953"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1106"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1811"/>
            <end position="1850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1900"/>
            <end position="1954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2338"/>
            <end position="2364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="39"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="83"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="180"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="347"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="438"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="456"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="959"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="991"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1025"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1047"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1122"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1811"/>
            <end position="1826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1836"/>
            <end position="1850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1905"/>
            <end position="1915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1922"/>
            <end position="1939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="2338"/>
            <end position="2358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4371355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="2741" mass="300810" checksum="A8903CF7B66EE6DF" modified="2023-11-08" version="1">MAPSNMFSYLRPGHAKRNASSLASPEPTFSPAAYPLPSPSHNTEYFSSPRFNDNASYTSSSPISPFPPQLPPIARVASKLDKSTSANSSEHTASQPISIARRDNALGAQEGEMLSPPQRAQEQLPPSYEYNPVSARPAMGTTPTGLRPRGLTAPQNTEYAQQSDSPSQSSTHLARPVLSPSYSNFSKSQTSLISGISDKLTGSSKTSSTPTVASSKGKSRLRNPMSLLMRRRSGQTLDPLSDESLVTPSVVPPMPDNYDPSIRGHIVHDFNAPRINRNFSYNNAYTEDRTPQDLGRVSPAKIDREHTPVFREHFDDETSYEQSQAAIRAEQLADKGFLARNSIQFSQPPPPLPKDSPPPPPEPYSTSSPPPRQHVGTSQSGLPASDLSPVQETSSPIDAPTEVTPRKRKSTKSPPPARSRATSVTDPSFQPAGLPAHFSSRASRFSFQIPGGSDSAQEKLLEERHKAKEAEKASKQVRMSTNTMDDEYDEYDMDDYDEDGGFDEEIPMLGEEDDFGGLGDQTLDSGINTFDFSSLSIQPGMNDAMSPVRQLQTPIDGNGNPIGFAMSGAMLQQFQSQAFGSQPSGQVAPSDVTHGLGLVGLQSGLDDPSPPVSVDVSAGPGPTTYNEQSPGNIDFDDDMYFDDGLIGDQEEDAVAEFDENVFDDPSGPLYERKVKFTPELDASRAPASYPPGLPYSETGYEADDDTMSKHLEKSEPSLAHKTSVAAQRNVPDFNNMNAYYSALADAANRAEASGRFARKASVDAGQASSDVEEYSSLSNSRPSLVPDDGRFSMETDGFPPDDDGFGMSSSFVDDYEYSDFDSALEDDPIIAAANAEALAYDDEGFYGQEFGFYAAAVGESPSAWGGFFGPSGLGRTVSGRNAVREPNLTPITERSEYSTRNSFISLNHFRDGQQPLQSPGLAQLARMSPYGWAEEEDMSLDALMKLRKGAFGGSAASLPGSTPQSPRNSSPMGMQLYPRTASAAGARMVEQDDSELESDESANISENLEGDEEDKRLMDAVNTAYEEEDGYEDDYEGSDGYERPESPTLTASDYNSLSSPASHNLAKDTPLLPPISELYAQHNVPLLHSPPNIPLPMSPYGMPLSSPFMSHQRPPAPPSINTSLSSPSATASTSTAPRRQSLGLISPISTSSPITPGGSGWRGGHSRKGSAADSVTGYSRTHPYGSNVQDLLRLDYLVSSRLPVMAILKSSLIFDRRILLGLAAAVILSFTFAFLYSPDLRPSADWIPPLTSKPTSTFSAPPKNAQDPNIVELDYLAIKPNVSDKIRFYWNIPYAANAGGKNRFRGPQPVNRTYGLSKGSGGDGEGPLVWDGSLGMCPRIQKIDATLRNKEDISGEDVLGIKTKFTEDCLSLNVFTPIDAEAGDDLPVLVNIPGGGFHLPGRSNGGEMVEKSRREKGADGKLDKGIIVVTMYYRNGIYGFLSGEEIAKDGDYNMGLRDQRAALEWVQKYIRYFGGNPDRVILMGTSAGGASVLLQISANEGDNNYPVPVTGRKPLFHGAIAQSPASPTFYTPTQAEKFWKEVADGLNCTDIACVRNSSPGDLYYENYPMSFPGRNTPPRWMWAPTTEPAGGMWTESAPAAIMNNRYAKVPTIIGFTSNEGTDQVKKTTDTEGEFKSYLADHYPLLTDDDLALLAKTYPNDRHWPDSGRYWDAVSKAQGDIRYICPTYLASNAMSTHNPSSVPTYQYQWDVMWGPDINNGFGTKHAGTVGQVMVKRPNEISDYFISFIKHLDPNVERTKGTAVWEKLDERKRRMYFTNIKEGGKRGDGENMLGKMRMWMEGWIRGGMEVTSTSNMAGRQQPTPSGSKSPLFEPEGSASPTLHDSSMSNPSASAAVMTPLTLGSMPLVKVEPDTDTPPFVQKLREIEAHAVSVMSGWEAQYRGGATGTTTVPCTAVTKSKDADSDSDNDGSESDSGSDDEDSRVPPLDPDKQIRPRLPIYHPGFKLTELLTKGVLSIFSQYITTAVRNGYKDSEANHLRTQVLKSRNIIYQESVRLAVAGDTGAEKSALLNSILGVVNLNIESDAGGACTCVITEFRQAPSTQTAPYAAEVEFFDLDVCRQLVKDLFTQWFKVKEKQKLNTDDVDDEDLNRMKTARDCVQQMFSDRLGHEKAEEFMGNATSSNDKTVLHELLKWTTDIHQQFVGKGHMPIEFESSTPENLTEQYHPFTREVANASFRGKPLGFSPWPLVRIVRVFLNSPILQQDGIVADVPGVSDTNYFRVENANRYLQSCDMTIVVGKIDRLQDNASFQQQYMDAFRRRRSGSVILVATRSDDLNEENSSTATLDTAAQEQLALVHEKISEVEQGIKMIDNEMDSNRLKKNTKANKRLKKRKKRLTRRKNGLEKQRKDVLIASRNKQVTLITGGNYREDTGDDAGAPMFCVSNRMYMRHLRGFDKTDEHSIPTMHLEQTQIPALCSYIYVLPSKGKTASLNHFINAFRTRVGELVRKFCATDIELLFGQLSDHSLQTKFDVLTSKNLENWEAMKKKNISTNWNNDLLSPVRPVIDAAFRTIIDQSTETFKAEAAQVSKEVIRELDATLKNDPHALACNAYDVCFRENLTLYYADVDRHVETAAKGLKTALIKVHISAVGLKKDHYFPDAMSRIYEKAWARKGAGKGQTMKDARCLYLHEAVPGPTGPFAEIASRAKDDASKAINTASEGLAASLDDMLRKVQVAFDMMKKKKESDTPEGKKFRTELHQLVDEARRILNGVAQESLELCKQYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNE9</accession>
    <name>A0A9W9CNE9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_003737</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4371964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNE9"/>
    <dbReference type="OrthoDB" id="47494at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47178:SF3">
        <property type="entry name" value="FAD-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47178">
        <property type="entry name" value="MONOOXYGENASE, FAD-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="6">
        <location>
            <begin position="12"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4371964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="409" mass="45681" checksum="090A34AB697E2DAA" modified="2023-11-08" version="1">MEGTTAAGGPRLPILIVGSGSTGLALAQGLRKAGIKCIVFEKNASQHRERDWNMGLHWGAPVLKSLMPDGWWDRIQSVQVDPHVPTKEEDTLTWLQGDTGEVMAAFTFGPFYRLRRSKLRDLLSEGLDIEYDKRLENITYAEDGQSVTAHFADGTTATGSMLVGADGARSSTRQVLLGPDVGAINHLPYAATFVQQRFSKEQALYLRSFHPLFLAGAHPSNTFAFFGMHDAADPDDPSSWTFFFYISWRSSLEEQEATKDWTDAQRLAQQKELSKPFCDPWKSAYEWTPDDAPVWHLGLTEWDPGREGHRWDNHNGLVTMVGDAVHPMTYQRGQGLNHAVTDAGKLRDALVQIQEGGDRAALITAFEDEVIKRGGTEVRDCTANTTLLHDWERVKQSPFFTKGMKKNET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPF7</accession>
    <name>A0A9W9CPF7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_001888</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPF7"/>
    <dbReference type="OrthoDB" id="508119at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000026">
        <property type="entry name" value="MFS quinate transporter QutD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF81">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="234"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="339"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="401"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="1"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4374811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="491" mass="53647" checksum="535BFAAD27F5CE11" modified="2023-11-08" version="1">MDAFQHSFGLDSKSPEFANLQGNIVSVLQAGCFFGALASFYVSDTFGRKWALIMADIIFLLGSIIQTLCAIGTTSLAQLYVGRVIGGFGVGLISAVVPTYIGENAPKEIRGRCIGCMQLFNVTGICLSFFVNYGINLDITSPTSSAKWRIPFALQMLPGTLLLIGIIFQNESPRWLVEKNRIKDAAKALAHVRGKPVEDHDVIQELDEIIADFNGHEKLPLVAQLKAACKGKSMIYRSSFVVILMFWQQWTGTNSINYYAPQIFKSIGLTGPSSGLFATGVYGIVKIVMTTVGLMAFTEQLGRKWSLIIGSIGQAFAMFYIGVNMAVHPPKGELDGNSIFAIICVYLFVVFYSFGWGPTPFILSSECSPNHVRSLLMAAALMTQWLFNFVIAKITPLMLAHITYGTFLLFGSLCILMGVWTVFCVPETKGVRLESIGELFEGEIVKGALGDIVPSRARAKQLKNHHVTDDLESVSGDGVGKGARVEQREDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPH8</accession>
    <name>A0A9W9CPH8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="5">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">DBP6</name>
        <name evidence="9" type="ORF">N0V83_003660</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="5">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPH8"/>
    <dbReference type="OrthoDB" id="3370at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17956">
        <property type="entry name" value="DEADc_DDX51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="194"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="534"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="18"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="506"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="27"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="102"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="511"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4373365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="709" mass="77908" checksum="18EF3B94909022EF" modified="2023-11-08" version="1">MPAPLFKRWVPPKQVVKSASPVDVVANSPNPSRTTAESTPVKTNLIVDETQMEPPKSIKKPKKRKRSAGANEEEEETSKKHKAIFSKFEKASKLAEARSQREEDEPTDGAEVQEAEELHDLQPMPQPAPVPEPAFEPTFSTLPTWLAQPVTVDASKTLPFTELGVDPFYTKKLEKQGFKEALAVQTALLPMLHPGFEQHLGDICVSAKTGSGKTLAYLLPIIEALKDRAVPVLSAIIVVPSRQLVNQALQVAEELCAGTKIKVGTALGNVAFASEQKQLVRLRAQYDPTAAKELHEKASRQLQTGSVERGGIFDDLKDMPLDHVPRYDSGVDILICTPGRLVEHIDNTTGFLLNAVRWLVIDEADQLLNQNFQGWATVLMGALHGETPVDFLSAQERIRKREGDANSIWSVALPARRQLTKVVLSATMEKDLTKLGTLKLKRPKLVVIQDEVAETQPLDNEENVFELPSTLDEFAVHVGDGSNKPLHLLHVLLNFVFPGIQTDVGAKEDNSDSDSDSDTDSSSEDSSDGEKDRSVADHISRVLIFTKSTESASRLSHLLAVLEPSFKPYLKTMTRALTADASRKLLKSFSTGAVKILIASDAASRGLDIPSISHVINYDIPTSITSYVHRVGRTARAGKQGQAWTLFTKTEAAWFLKQVAKGDSVKRQNKKVKRMEWKESTVTANGKKKTYRAALKQLESAVTGHAEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPM6</accession>
    <name>A0A9W9CPM6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hym1p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">HYM1</name>
        <name evidence="3" type="ORF">N0V83_002085</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mo25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPM6"/>
    <dbReference type="OrthoDB" id="609103at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000257">
        <property type="entry name" value="Conidiophore development protein hymA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013878">
        <property type="entry name" value="Mo25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10182">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10182:SF3">
        <property type="entry name" value="PROTEIN MO25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08569">
        <property type="entry name" value="Mo25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="639" mass="72131" checksum="F59A6D8BF8C9F085" modified="2023-11-08" version="1">MMELPHDSKREATILKIAASDRVLPLLDTFPENGSRFVLVFPFLRYDFSDLLHDKKLSKKQIQACLRDLFSALEYVHSQGVIHRDIKPSNILLKSLDGPAYLSDFGIAWAHDVSETETADSKITDVGTTCYRPPELLFGNKHYSCSLDLWAAGCTVAEALAPEHETLFDSGELGSDLALIQSVFKKLGTPNLNVWPEAANFPDWGKVQFYEYPAQPWSALLPSSSETEQDLVSQLVKYESGARTTASKPLTSSSTSHPDNYRSGGFPISLNMAFLFRNKQKSNLELTRSIKELTQRLAQEDKANPKLEEGLALDLQQMKIRLQGTPDTEVNPEAVFQLLTNILNEDLLYVLAVNIHKLPFESRKDAQVIFSTAFRYKPAGASDPQVLQHVVQYRPDIIIALCRGYDRRESAMPCGGILREALKYDAIAALLLYDEPMEDGKSVDLGSVNPDMPSSGNGVFWKFFGWIDKGAFEVSADAFNTFREILTKHKPLVATFLQTNFDTFFTKYNAVLIQSESYVTKRQSIKLLGEILLDRANYNVMTQYVDSGEHLKIIMKLLRDDRKMINYEGFHVFKVFVANPNKSLAVQKILISNREKLLRFLPSFLEDRTEDEQFIDEKSFLIRQIEQLGPMPVVPPSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ36</accession>
    <name>A0A9W9CQ36_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">U3 small nucleolar RNA-associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">UTP4</name>
        <name evidence="2" type="ORF">N0V83_002920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ36"/>
    <dbReference type="OrthoDB" id="8883818at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034455">
        <property type="term" value="C:t-UTP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046351">
        <property type="entry name" value="UTP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44163">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44163:SF1">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="572"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="630"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="749"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="587"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="632"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="914" mass="100922" checksum="9C073855589B5889" modified="2023-11-08" version="1">MDIHRARFVPYTSSAINALAFTHTKAEHGNHEPESLRLAIGRANGNIEIWNPANGAWLQEKVFFGGKDRSVEGLAWTQEPDHKDADNKIISGRLRLFSIGYSSSITEWDLATGLPVRHSNGNHSEVWCFAAQPRKSNKATGRAKEGSFAHQKLVAGCADGTIVLLSTEDDDLTFEKFVSRATNKKARALSITYKDDDIVLAGFADSTIRVFDTRTGNVMRNISLGSGPQSGLKEILVWKVKCLANGDFVSGDSTGDIRIYNGKTFGQMQRISGHEADVLDLAVSQDGMSIFSAGMDRRTCYYTSKKGAAQGQSGKWRKVSHQRYHEHDVKAMATYEGNKLSVMVSGGIDTQPIAVPIRGFGKELSRGLPTLPSTPPLVGAPGARLLVSWWNTEVRIWRVKAQVDGTEKPRVVARLALQGEENITSVSISRDGGLLAVATVSEVKLFQLIRPQPGASTGLRIRKLDMPSVDGARMVTLAENGKWLAAITATNDVQLVRIVRTDDSSDLPRALNAVQHLTRLHRDNFSQNTLNGPSGSYERSIAHVDFSTDGNVLAVVDLAGYIDTWVIEGHEDSTAPEVDIDASAPAGDEDEESDDDEDETKAQITYLGQRWTQNPSGRLLPRLDSTPVLLSFQPNSDESKRPQPNGNPAVHPTRHNPHPHSHELPATDYRLLLVSAEHQLYLFDVMTGRLSEWSRRNPLSSYPREYQQLDLPAKGCIWDFSSPVQRIWLYGEKWLFMFDLTKDFPIADSEKSGMSKKRKREAAKDHSGAGSVIPQKEAPVTKMRKFQGDQEDQTSKIAWFDVNAASSSTVNDGADDDIEDGSQPLASLRRTLTEDGESLPNGASEHTTEGGEVGEVVDQRKANEPWWHTFKYRPILGIVPISGNDNYHAPEVVLVERPSWDLDLPPRFVGTHEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQB9</accession>
    <name>A0A9W9CQB9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">High-affinity nicotinic acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TNA1_1</name>
        <name evidence="9" type="ORF">N0V83_001322</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQB9"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000057">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF54">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="439"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="42"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="10"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4376041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="482" mass="53625" checksum="6C8F19777B88FF01" modified="2023-11-08" version="1">MLTDEKMMAETTTKSSGSSDQDLAEPTTVTPPSYTLKLDLRLIPILGCTYTILFLDRTNIANARIEGFEKSLNMPSNGYNTALWVFYIPFVLVEVPSNMIMSMPRVRPNIFLGCNMLVLGVVAMCQGLTGSYGGLLALRFLMGIFEATLPAGAALLVAEYYTRSQAALRFALFFTFGVLGPCISGLLAYGIRNMDGIQGKEGWRWIFILEGIFTIAISFLVFFLVPNFPEKTNILNAAEKKHLLETLRRDKGDQKLDIKSVNWLKTIGDYKIWFPTLMFFCCDMTAASMSSFIPTILTELGWTRARAQAMSIPIWVTGMIFQILGALIAGRTGWRFPFILFGILMVLIGWIINMVYSEGRGGGISAAVRYFSLFCMSAGTFVQMSMTTTWLVNNLRGRPSIAVGTALILGIGNCANFVASNVFIKTEAPFYPTAFRTGLAITITGAFSCLVYVGLLWRHNQKLVVKRELGGDDDQKEYRYHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR34</accession>
    <name>A0A9W9CR34_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000351</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR34"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="467" mass="51554" checksum="656309FB62170FD2" modified="2023-11-08" version="1">MSNNTFTYGTLPSFTFPDPGPPLPPSSNAYITLNPPGYPKPDLQWYLLFAISNATMHPRSGFGSTYASIRTHDTIEPRWSEPAHDYVTNGSLILYCAVCKYPDSYEARSNDEVWRACDYYDSDEARLNADIMQQTEYDIGPNGGEDVTIEIGKPVIPVSSSLYADVARRGTNRTMACMAALRLNENLNPERSENSMVMSQLFLVQDTPHGDESNWFTDEDYLPHLYNSTWPGGIADETRKGLGKATPSSTRESATATATCGAMRSGDDCGGWANWDSGKKVGVILGALSGVGVLLLCYLFYWRAERYQSSGRGSGGQRRRPRPMLLSEYRAQQRREREEQEARGRTSYYDGVARLEREAAAGRADAGAGRRSGDDAGGESDKPPPAYNEVVSAQEGMLASYAIRRDERNGNADAPSSSPPPLAAEHERSQATEEDIARPTTPLPSYHPSSDQLMDRSRREYRSSIDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CS16</accession>
    <name>A0A9W9CS16_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Phosphatidylinositol transfer protein SFH5</fullName>
            <shortName evidence="16">PITP SFH5</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">SFH5</name>
        <name evidence="19" type="ORF">N0V83_000491</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Non-classical phosphatidylinositol (PtdIns) transfer protein (PITP), which exhibits PtdIns-binding/transfer activity in the absence of detectable PtdCho-binding/transfer activity. Regulates PtdIns(4,5)P2 homeostasis at the plasma membrane. Heme-binding protein that may play a role in organic oxidant-induced stress responses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(out)</text>
            <dbReference type="Rhea" id="RHEA:38691"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:38692"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 16">Endoplasmic reticulum membrane</location>
            <topology evidence="2 16">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Microsome membrane</location>
            <topology evidence="16">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the SFH5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CS16"/>
    <dbReference type="OrthoDB" id="75724at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017157">
        <property type="term" value="P:regulation of exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000018">
        <property type="entry name" value="Phosphatidylinositol transfer protein SFH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042938">
        <property type="entry name" value="Sfh5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47669">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN SFH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47669:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN SFH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12 16" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 16" id="KW-0492">Microsome</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="18">
        <location>
            <begin position="231"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="76"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="37"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="77"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ4377663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="374" mass="40789" checksum="CB26370424EBBB00" modified="2023-11-08" version="1">MATKAEEVNTTPTEAAGPVGSATTEKQAETVPVVESAAEKDQPVTEKTGVEDATAAVAELSVADKDVAVKKTEEDAAAAVTASAPKTTTDPAPSANGPTWPETSPDHPLTKFFDLFETLVTEAGHNEVYGVELSKSNPFHTKLILQKFLRANQNDLEKAKLQLLETLKFRKEFDPVKAAGESFEQSRFGGLGYILEVEGVPESPNKKDIVTFNIYGAVKDNKATFGDLDGFLRWRVGLMEKSVQKLNLSSATEPIPNYNEGPDPYQGFQIHDYLQVSFLRQDPHVKAATKKTIEILGRHYPETLSRKFFVNVPVVMGWLYSAMKMVIAKETVKKFTVLSYGNQLATELGPNIPKVYGGTTGDLGDVGEGVKFTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CS20</accession>
    <name>A0A9W9CS20_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">IMP-specific 5'-nucleotidase 1</fullName>
            <ecNumber evidence="12">3.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ISN1</name>
        <name evidence="13" type="ORF">N0V83_000506</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">IMP-specific 5'-nucleotidase involved in IMP (inositol monophosphate) degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>IMP + H2O = inosine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27718"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="3.1.3.99"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ISN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.-" evidence="12"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CS20"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006190">
        <property type="term" value="P:inosine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071590">
        <property type="term" value="P:nicotinamide riboside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071592">
        <property type="term" value="P:nicotinic acid riboside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009453">
        <property type="entry name" value="ISN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28213">
        <property type="entry name" value="IMP-SPECIFIC 5'-NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28213:SF1">
        <property type="entry name" value="IMP-SPECIFIC 5'-NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06437">
        <property type="entry name" value="ISN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028836">
        <property type="entry name" value="ISN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR028836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4377678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="429" mass="48294" checksum="1189822FB6E51A99" modified="2023-11-08" version="1">MDQSKITHYDAYIPVPDIHEGLLAVPFVLHSHPTAVFEPDGESVDHQANIAQRRYAEIMRDVEEIINDHSKPIFVSLISNYGKARILEAGKPDRSKLKLLVPSVANFFTPLALHDAFIWQDQRRFISHRRFVPPSFNDIRLVLNTAQVMSLVRGGPLDLVTFDGDVTLYDDGQCLTPENPVIPKILHLMRRGSKIGIVTAAGYTAAKRYYERLFGLLNAIQASDLPLELKQNLIVMGGESNFCFKFDGNSPDMLKLVPREEWLLKEMLLWHESDITELLDLAEASLKDTVRNFRMEALVVRKERAVGIIPKGGHKFCRETLEETVLIVQKILEISEVGQRLPFCAFNGGNDVFVDIGDKSWGVLACQRIFGGIEGGKTLHVGDQFLSAGANDFKARLACTTAWIANPQETCQLLDEIAELDEMQQRKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CS46</accession>
    <name>A0A9W9CS46_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MFS general substrate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_000583</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CS46"/>
    <dbReference type="OrthoDB" id="10262656at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF6">
        <property type="entry name" value="MULTIDRUG TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G08740)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="354"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="413"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="475"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="553"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="311"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="311"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="597" mass="66170" checksum="04D83E6B6BF39652" modified="2023-11-08" version="1">MDHYENQEEDKTVLRTYTSDSDNEEFNTHPERSRRRSRELLIDTSVRAGTALPQIDDTEQTEATKEKPVSWRALPRKDQLFVLTLARLSEPLTQTSLGSYLYYQLQSFDPSLPESTISYQAGIVQAAFPAAQFLTAILWGRFADSEHGGRKRVLYIGLLGTMCSTIGFGFSHNFAMAVAFRSLGGVLNGNIGVMRTMISEIIKEKKYQSGLLANPVASYPSLFGPGSQLGGKDGVQWMTRWPYALPNLMSAVFLFFSSMSILLFLEETSELCKHKPDFGLRIGRWIRRHILRQRIAPEAGYSSIPEDEETVATSSLELQPTPTSAHPGTPAPPDRLEKGLRQKLPFRRIWTRNLLITLCAHGLLAMHVGGFNSLWFIYLSTPRFDPSNPHPPGFKPHGFFHFTGGLALPPARIGIAVAILGVIGISLQLFFYPKLSHKLGTVKSYRIFLALFPVTYTLTPFLSRVPSWSKPPEGVSGPFIWMAMTAVLFIQVLARTFALPCTTILVNNVSPHPSVLGTVHGIAQSVSSLTRTFGPIMFSWVFGRGLDMGIVGFAWWLMAAVAIFGCAVGQGVREGDGHEVLMEGEVKGEDGVVRRMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYM8</accession>
    <name>A0A9W8XYM8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010286</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYM8"/>
    <dbReference type="OrthoDB" id="1103324at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050364">
        <property type="entry name" value="Cytochrome_P450_fung"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46300:SF6">
        <property type="entry name" value="CYTOCHROME P450 2C30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46300">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4363166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="542" mass="62408" checksum="35B4A3D9BF95E919" modified="2023-11-08" version="1">MHALTVYALGVAIIGFVLYTFVGAVSKYNAKHNPRGLPGPTLVPWLGRVHDLPIEFMWLKFKEWADIYGPIYRTKMLGANFVIIADEAIAEEMLVKKGKVYSDRPEIKSLFDAKSTYGSMEYLPLMGKNQYWARQRKFTHSYLTGATNAQYYGIMELEAKRWLYKLVENPDDFAFSLEDMCSKVMCQLTWDDDSLSVPLTPSAWGLLTQMSPAGPITNVLTPLWDYLPEPINPWKIRERKRHDEQQAWFMDCLQKTRKLMEKGKQRPCFTRTYLETAEKTNISGDYEASSVIGMMALVGIFTVAGPLYYFLIAMVHHPEWQHKCQEEIDRVCGSRPPAIADMPNLPILRACIKETMRWKPTVPTGVAHECEADDWFRGYFIPKGTRILPLDWAFLRNPAKYPDPDSYRPERWLEADWPTYQEPLTKYPDIVGMTSFGWGQRQCLGMSITRDETVTGCGGLMWAFNLKRKVDPMTGEEIEVPLNKSNSLLIIKPDLFEMAFEPRSEARKAEVIGQWKAAEAEDIEQRAEFLRQAETREVLNRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y032</accession>
    <name>A0A9W8Y032_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009841</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y032"/>
    <dbReference type="OrthoDB" id="3784117at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="216" mass="23248" checksum="3B0366F540AF992F" modified="2023-11-08" version="1">MITDKSQTLGGNPPVTHPPVASTLLERRQNRPGLVARISLPSTGIDDEVLASPPPTQALLSPVPAANKLHAGHTPLIPRSLSPVRGNDEQPAEREPVAEERIATPEDDGGLKGPLMLPTNPVDGAADHIALDVLDDELEKIAKEQDRFSKLRDAPEPTPVDTHEEEVPLSRKESSESRVSSRDVAAVDGVRLKSPPLNFGLPIGQAPRRRSETSER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0E1</accession>
    <name>A0A9W8Y0E1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_009332</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0E1"/>
    <dbReference type="OrthoDB" id="16820at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051104">
        <property type="entry name" value="FAD_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46720:SF3">
        <property type="entry name" value="FAD-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46720">
        <property type="entry name" value="HYDROXYLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01460)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="4">
        <location>
            <begin position="141"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4363880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="416" mass="44896" checksum="0A0F8A0292B8B6C7" modified="2023-11-08" version="1">MAETRKIQIAVSGGGLAGAAIARALYQQAHIEVNVYESAPEFLEKGMAIGMAINAQRALSKLVPDVEEMFQRAGAVTLNSSRVMLAAGPHAGMKVIDVAEEKPGKILHRAALLHELLEPIPKDRLQASKKLVEIEEQQERLLLKFDDGSEVQADALIGADGIFGSIRAHVLGADHEAVNPVAAGWAGAMNMVPYPKAEAKLGAEILNENRQYGWVGDGGIFIHDSIMGGKMVQCIGTSVNGDTSGARRIPIDREYMDKAFASSLDGPVAKRMIDLLLDQESPAVFAQYEHTDAPTYVKGRVCIVGDAAHAMTPWQGSGAATALEDAVILGTLFEQIRSPDEVEQVLETYDALCRPRSQRIAESSRQTGRILSGIADDIGLDPVKMHDALKDRWSFIHDFDLDGHVESAKASLENKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0I9</accession>
    <name>A0A9W8Y0I9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010332</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0I9"/>
    <dbReference type="OrthoDB" id="194468at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050287">
        <property type="entry name" value="MTA/SAH_deaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794:SF11">
        <property type="entry name" value="AMIDOHYDROLASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794">
        <property type="entry name" value="AMINOHYDROLASE SSNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="2">
        <location>
            <begin position="36"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="325" mass="35006" checksum="0E8846AC13401300" modified="2023-11-08" version="1">MIGKLVKVEESNKFGLLDPRDRDVSSMSIDAAIAAHEKHHGSCDGRLHVWMAAGTPRGSAERDHKAIGDACSEHGIGLTMHCAEAPKDLVIYRESYNCSPVEFCYRTNLIGKGRKTVLAHMVNLDLGKDLPLLRETGASVAHNASSNCKLGSGIAAVPELLEENVNVSLGTDGAPCANTYDMIQEMRVSALVHKGSRQDAAAITAEEVLTMATINGAKALGLETEIGSLEVGKKADFVVIDPSGLHCVPFDPEQILDGGIDPVTTVVFSCSGADVDKVLVGGEMLVDEGRLVKCDEKQIREEAQRTIRRIRNKSKISSKLKRKYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0L6</accession>
    <name>A0A9W8Y0L6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger transcriptional activator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ARO80</name>
        <name evidence="4" type="ORF">N0V83_010412</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0L6"/>
    <dbReference type="OrthoDB" id="2262349at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009074">
        <property type="term" value="P:aromatic amino acid family catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.10:FF:000012">
        <property type="entry name" value="C6 transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052780">
        <property type="entry name" value="AAA_Catabolism_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31644">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR ARO80-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31644:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR ARO80-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="27"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="65"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="182"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="818"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="188"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="869"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="885"/>
            <end position="896"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4362319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1016" mass="111686" checksum="F6091925C2A9498D" modified="2023-11-08" version="1">MSTTGSNASPQPRSQPSQSQHTRTYQACIPCRRRKVRCDLGPVDHPHDPPCVRCRRESKECFFSATRRKRKADGEDDFDEVDDYEVRNGRKRQRSDDVRPEPISIPASSNPAYISQPLTPGGSVGRYEPLRRPNTSAGHQPLPNEEEDQQVNNETVATLQTGEVYSGHDALNLLFEAAGMNGHHHRTGSTSSMPRPNMGSTPGSQANFASPQANTTHVQSNSRAAADMTVDPALQHPTLPDPPNEVAFAEAVRAWSKFRFVRAGWLTAKEAIAYIDYFYAFLSPLTPIVVPDYRDHAFHAKLLKEEPMLVITLLTISARYSQPSGSNGVGATSRAYLIHEKLWKFLQGMIDRMIFGQEQFGGGFCGAGQQPGSDVNPLSRKGLRTLGTVESLMLLTEWHPRALHFPPGDDDDELVLPDDPYEAGPKADMYKGVGDKRIDSWLEPCWRSDRMCWMLLGNAMTLAFEIGVFDETSETEFEEANQHLSHATVKAYYRRKNHLRDLLIIYVTQTSGRLGLTSMLPKIEREDESFIGGPTPLEKYQERISRIKAPPPQFGMRSDGQRLPLESIATQERHAVQDLVLDFWQRIAKVMEMGNLKLFSNRRKTRELLKSGRYEEMLKSFQGPILEWKTCFERAPQVPTQLRHVLTIEFEYTRVYLNSLALQAVVERCTHNTPLQTHAQPNGLNGRTTNGNDDGGAIPFSTLVKWYANDRHYINEVIDASRNVLKVVVDGLHPGGYLRHAPVRTFFRIVSVAIILLKTFALGATEEDVAVSLGLLSDSVDALRTSIVDDVHVGNRFADLVETLTHRIRSRFVRLAANGSSGSTGISRAGSEGPLSTQPLMPPPAPNHLGQQQQQQQATGHHLAPPYMGSGAQAFQGSGSSVPGSPGGVGVGGLPGGSGRATPLWGISAEPYDPNSNSISIMPPPGNYANFSNIASSSSSNNNNSSSSTNWSQWANSGASWGAGNGQDQDWLALPLDPLLDQWGAEINQTAMGPDIGGMDMLDILLNMGGPPGSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0Q6</accession>
    <name>A0A9W8Y0Q6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">CTP synthase</fullName>
            <ecNumber evidence="3 12">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">URA7</name>
        <name evidence="15" type="ORF">N0V83_009732</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 12">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the CTP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="3 12"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0Q6"/>
    <dbReference type="OrthoDB" id="1739076at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097268">
        <property type="term" value="C:cytoophidium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000207">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000005">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003792">
        <property type="entry name" value="PRK05380.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF0">
        <property type="entry name" value="CTP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4 12 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 12" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP synthase N-terminal" evidence="14">
        <location>
            <begin position="2"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase" evidence="13">
        <location>
            <begin position="299"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU810713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4363439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="577" mass="64174" checksum="5A58B551470B65FE" modified="2023-11-08" version="1">MKYVLVSGGVISGVGKHVSSIKVDPYLNVDAGTMNPKEHGEVFVLSDGGEVDLDLGNYERYLNITLTRDNNITTGKIYQHVIERERKGDYLGKTVQVVPHITDAIQDWIEKVARIPVDDSGEEPDVCIIELGGTVGDIESMPFIEAMSQLRRRAGRNNFMQIHVSYVPVIHGEQKTKPTQQAIKAVRSNGLIPDLIACRCERELEASAVEKIAHFCQVDTEQVLVVKDMPSIYQVPMLLEQQRLIPMVRQFLAFDQVTISPAMVQKGSHIWTQWQSLTGHDTRYFDSVTIALVGKYVELQDSYLSVIKSLEHATMRCRKRLDIRWVDSDHLEPKCQSSDPAKFHNAWHEVVKASGILVPGGFGQRATEGMIAAATWAREHKKPYLGICLGMQIAVIEYARNVCGIKGATSEEFNGDAADKLIMFMPEVDKTTMGASMRLGLRPTLFQPGSEWSRLRALYAGKTEILERHRHRYEVNPDYIETLEKGGLSFVGKDEAGVRMEVIEIKDHPWFVGVQFHPEYLSRVLDPSRPYLGFVAASAGMLDEITGAYQDGAADGTAAEGIANGVGGLRINGEASF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0R5</accession>
    <name>A0A9W8Y0R5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009747</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0R5"/>
    <dbReference type="OrthoDB" id="3795318at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="408" mass="46074" checksum="87A07C907E53C537" modified="2023-11-08" version="1">MDVSAGLALYDPNLAVTTSAMQTGPYISRLAQMMTRDRSITWPVDAVDPMCRASKTCKSYLIAGPYRTVQPWPFTAENETMDGFRLYNTPFYQVDMWDVEREWDLSFNETSECSLYGGFDAAWDYSLEICMKEYAKDVLVAGWKSCQSGYSNTTYQCLDPGSLPSGKQGWTTYLHFYRRNATVVFSRTDFTILDATDFSPPQSQNISAASLFQSVNAILYRPNAPANNFQYDRNSQPYVLTQIIGSDLWRSLNVRMNGLAIGRDWLRNILILPVYLFQATVIALNYNIPFRIENGTTPLVNLPQENYVRGSYCIVRTQAVPGLETVIGYACVAGFVLLFVGIAKLVAFQWPEAGTSEFPLLDYEYKTVLAEGRHEASLRQKFVASQRKYDNSAILDEIADLRVGLRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y157</accession>
    <name>A0A9W8Y157_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">sterol 22-desaturase</fullName>
            <ecNumber evidence="6">1.14.19.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_009935</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.41" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y157"/>
    <dbReference type="OrthoDB" id="1372046at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000249">
        <property type="term" value="F:C-22 sterol desaturase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000021">
        <property type="entry name" value="Cytochrome P450 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF228">
        <property type="entry name" value="C-22 STEROL DESATURASE ERG5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="517"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="481"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4363639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="540" mass="60455" checksum="67C3653711D4F7DE" modified="2023-11-08" version="1">MATITTASVEVVKGNVSQAIQNGTSSRIDGHFSDMLRGWSSWQIILTTLLVLMTYDQVMYIKRKGSIAGPSFKIPFMGPFIQALHPKFESYLAQWASGPLSCVSVFHKFVVLASDRDLAHKVFKSPTYVKPCIVPIAEDIMGSSAWIFLQGKAHAEYRRGLAGLFTPKALSTYLPVQEKVYSDYFDKFLTHTEDNGGNPTKFMAFFREINCALSCRTFFGDYISQDAVKKIADDFYLVTAALELVNIPLSMYIPFTKPWLGKKIADAVQAEFAKCAAACKANMASGASPTCIVDQWVLHMMESKRYHSRIAAGEQNVPKPTNLIRDFTDAEIAQTLFTFLFASQDASSSATTWLFQVLAQRPDVLARLRQENLAARGGDKNQPFNLHMYESLTYTNAVVKELLRHRPPVIFVPYITTKAFPVTPSYTVPKGAMIVPSCYPALHDPVAYPQPEVFDPDRWITGDAESKTKNWLVFGAGAHDCLARKYVPLTMAHMIGKAALELEWVHHKTERSEEIRVFATLFPMVCSLLFCWTMMVCGFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1L2</accession>
    <name>A0A9W8Y1L2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleoporin NUP37</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008239</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1L2"/>
    <dbReference type="OrthoDB" id="5323870at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="437"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="660" mass="71138" checksum="7F3B6C45D45FA81F" modified="2023-11-08" version="1">MKPIIATKGKLTQLRYELPHRAHDSKIYPVKAPNGSTIILYGHETGVGIVWRGGRPLKKSAPPPKQPAKVNGTSNDAIMIIDSDDEEPAKAASQQPAPEAEFEEEEEELDPDRPYPSIIQHLRLSLNTKVLQVAVPQIPTVSSLRPADTIPAIFSKKMVFTVACADSTVRVITLPLSPLPDAAKEKPLSANSQFGEQIVKIHGHQSITRGVTMTWTSNAEPISKDRPADDMDVDREEDATATPGRRRRKQQSRSRSRPRTEEGFALLVASHSAELGGVLKIWRFGLAETSLNVAHPIAPYKVLTLQKPASRVAFCTAQYPKSRHSQLLITDSTGTARIYDLFASPSRKRRASGGQDEPGAFVGTFRSTFEGVKNNAYTPAVLARRKPIIDAAWASDGHHILALLADGEWGVWDIDRSGPSPPTNPSAFSLRGFVGTSEKEGSASGASSPKRSGRSSLAPMTPNTRRRKEETLFHGSSSSSAVATRGGISIASSTSANGHTSEDSVVIWYGSEVYRIPDLAKFWSRTASASGGGSLPGPGLQVYDVSLLGEAITSISQFDTSTQASRMAIARDALVSAEHRLIIAVNTNQPLGRDLNAMFAQEQVDDEETRRTDQALLSQGELDLGGMDRLLENMEDSGTVSKTLTLGGGPRKVLFASSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y270</accession>
    <name>A0A9W8Y270_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribosomal protein S23</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps23</name>
        <name evidence="5" type="ORF">N0V83_008832</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y270"/>
    <dbReference type="OrthoDB" id="1713912at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03367">
        <property type="entry name" value="Ribosomal_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000007">
        <property type="entry name" value="40S ribosomal protein S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005680">
        <property type="entry name" value="Ribosomal_uS12_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003254">
        <property type="entry name" value="PRK04211.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00982">
        <property type="entry name" value="uS12_E_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002133">
        <property type="entry name" value="Ribosomal_S12/S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4365214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="145" mass="15919" checksum="073233500277E78A" modified="2023-11-08" version="1">MGKGKPRGLNAARKLRNHRREGRWADLHYKKRLLGTAYKSSPFGGSSHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKVTAFVPNDGCLNFVDENDEVLLAGFGRKGKAKGDIPGVRFKVVKVSGVGLSALWKEKKEKPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2A2</accession>
    <name>A0A9W8Y2A2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_009904</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2A2"/>
    <dbReference type="OrthoDB" id="5363962at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003939">
        <property type="term" value="F:L-iditol 2-dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006062">
        <property type="term" value="P:sorbitol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05285">
        <property type="entry name" value="sorbitol_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045306">
        <property type="entry name" value="SDH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161:SF7">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="9">
        <location>
            <begin position="30"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4363608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="393" mass="42061" checksum="63BF3F1351AB497C" modified="2023-11-08" version="1">MAPSALADIYPIPARHYSKSSTVVACVLHGPRDLRLETRTISDPGPNELQIAIKATGICGSDCSYFNKFRNGDLCAVEPLSLGHESAGVVVALGQNVTGYQIGDRVALEVGVPCDNCRSCQRGRYNLCPKMRFRSSAKSVPHFQGTLQERINHPAKWCHKLPSHVSMESAALLEPLSVAIHATRRAQIEQGDTAIVFGAGTVGLLTAAMAKVSGATTVVIADIDHGRVNYALTHGFAHKGYIVTPRKNVGETAEKLAVAKELAADVMQIASLNDIDFEGADVTFDCTGKEICMQAGLYATRPGGKLVMVGMGTPIQTLPISASHLKEVDIIGIFRYANTYPTGIKIISAGVLPNLDNMVTHRYKFGSVAEAFNTAGKTKDDSGKLILKVLVEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2K3</accession>
    <name>A0A9W8Y2K3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007909</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2K3"/>
    <dbReference type="OrthoDB" id="3749155at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040966740" evidence="1">
        <location>
            <begin position="17"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="269" mass="28312" checksum="995D33D3CB2E6BF2" modified="2023-11-08" version="1">MRTDLRIFALLGLANAKGLVSRQDTQAADFNGGPSAQAVNGRGPWRPITNSPSFFSLEVDAQQCAAGDADPVNDCPQGNLFVRLTNGEVIVTPYNKFWDPKLPIFFVDDDSKVYTVSKNPLQLYVDSDTGAVKYAPVGWVPPTAITTGFFHTGTNPLGVVNPSSSYLGWPLPNGLLYSLNNAVTPFNLCPLGITGQYQLFVSDANFANTDGATTSAGGVDKQNCLAFNLAALDANPFARSMQRPQTGGAPYNGNGGTGVVVGAGTAGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-06-18" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2T2</accession>
    <name>A0A9W8Y2T2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_008836</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4772757at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000089">
        <property type="entry name" value="NIF domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF15">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="FCP1 homology" evidence="4">
        <location>
            <begin position="329"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="154"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1186"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1347"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="22"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="197"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1199"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1209"/>
            <end position="1225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4365218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1397" mass="156046" checksum="0F12302BC76A8F00" modified="2023-11-08" version="1">MNSLSALANIGTPTPTPPRSRAGSQSNILEPRSNDTQSSNDASARIANQSETGKMGVSRSDGSGYSTTEAVASDYDEKTPLLNQSSKGPFEEAKPRRRWLYPQRISQGIVAVVGVLAAPFVYTGQRLVACFYYGVDGTFSLLAPVYHITRTFTRGRRKKVKTQATSSSASSNSNEKGTRKSRGSSTAHSQPDKSSRRSLSIASTSTAITSDSESERPPTRDEDMDSPSRHTRSKSNASSGGDEIAPAKRSIRIKLHNEDALRQRKAAKRAQTSKSNSVSPEAAAALKSPTGPVTAASKQLTKFPRAPQPPRPLVPRRQPSYSASGPSAVGPHQKTLIIDLDETLIHSMAKGGRFQTGHMVEVKLQASVGAGGQIIGPQVPILYYVHKRPYCDDFLKKVSKWYNLIIFTASVQEYADPVIDWLEVERKYFAGRYYRQHCTFRNGAYIKDLAQVEPDLSKVMILDNSPVSYVFHPDNAIPIEGWISDPTDNDLLHLIPLLEGLQYVTDVRALLALRLGMPSSDYSLFDEAIEAVQANVLEEHRRYFHSQTNASLFLDELRITSASNGTSNRRLAECCRKFGLFSRIFAPYFDILSICVHIRPEWVGCFWGTVRLVFKASSDFPILLEKIADMLEAVAHILPPYQQIFETCEQRDTFSLQACTEDTRLAALLSYVYADLIQLFLDLYRVFCRGSQEKETENQIQNYDEIAQRRNRYLTFLNRQQGVNGHNGEGLGDQRLEKRLRRVEKIKLWLSSGPSSVEVPNSSSRQQSLSSCAWFFETPAYLKWKNGSFEKSKANDKNDLEDTWQHRVLFVQAKPGFGKTFIWNTVIDQLSTEAEAPEISDEPPSTAFFQVHNRPDGGNSDGPFRALACQLIHTHRHDHSTLDAVCLLMRKTSFRENATTDEMLDVLSLLLRQHPTYLVIDGIDGCSDAEAFLASLAGLCRRSDAKVIVFSRPNIKIPLEYQKWASDAPHILPLTIEHNASAIEHFVAQDLNRMADQGFFGISMDRTSMPQVARIARGEFLWASMLLKFLRSTALSSDERRAILENIQSLEGLESLYQNILSVLERRSKQEKRIIADVFRWLSFPINQLSPPALRAALSTFDIGESEESCLSDVLDTLPELSCGLIDVADMNITFAHGSIREYLQTHLSSQNPYFSLYDEASVHAHLASRCLSYLAHDVPKRPLGGLSPRIRPALPTVPTDSGVSLRTSKSGDSGYKSRSSSDGGNHLLQPTVHNHSNGADTHSIRVIPFDTNLPFLRYAALCWPIHLSRALADSNAPSRLATPHESYSIVPYLPALSAFLTSRLAVAAWVEASFRYNLPPTLTRLVGPLSDLKGEIPPATVEGKELRLVVNELRELSERLVELKREHVEIMRDNPSLIWQIDGDNYWPVWDEIREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2V8</accession>
    <name>A0A9W8Y2V8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007737</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2V8"/>
    <dbReference type="OrthoDB" id="4850726at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF64">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26639">
        <property type="entry name" value="Het-6_barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="48"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="660" mass="73963" checksum="097276AD6461BF90" modified="2023-11-08" version="1">MAPYSYQLIDESNHEIRLMTLLPAQDRESEVVCDLNNVRLTEDNVPAFEALSYTWGPPANPSTLRVSSLTNESIEVTRNLSEALPYLRDPEKPRILWIDAVCINQKSLAERGQQVQRMASIYSLAERVIVWLGLDDTDTNLAMAACGLLDSKVDTNVGGYAVLKCRTNDPADAHWGDKHAPLPYDGATWQAIAKLLRRPWFSRLWIWQEIRLAKPNAVITCGTDTMPWMHLRKALDCMVYKRVPFDTIGVEGLGALVRNGFDLATTYGYITLKRLLTLMRHAQCTDSRDRVYGILGLSSNTDAHINVRPDYTKSVADVYMELLVCYMEKSQRLELFSLCELDETLHNWPSWVPYLEKPKTTRHLTHSHSSGHSPCSAERLDYGVLKLTGIITGKVSKVDRLITSPRTEPDLSYAVLVEAIQRWVTWFDMASAYIDGNPMAEALCRTICAGAISDAYVPPHTAFPSMESSRIVLRDIMESSYAQSSNSPDKKLFLEYGFKGCNGRAFFTTAAGHVGMGPAGTRVDDKLCVFLGCDFPMVIRPAENGRFRIVGESYVPGIASNEALLGRLQSNYRAVVGYDVGSGLYNWGFFDSQTGKVQVEDPRLCLDLPQGWRLANHDLKRVWERFVHDGAPAKLENLDPRLSKLALLSRGVPLENVEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3T9</accession>
    <name>A0A9W8Y3T9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA-directed RNA polymerase I subunit rpa49</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RPA49</name>
        <name evidence="7" type="ORF">N0V83_007724</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPA49/POLR1E RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3T9"/>
    <dbReference type="OrthoDB" id="532500at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009668">
        <property type="entry name" value="RNA_pol-assoc_fac_A49-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14440">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06870">
        <property type="entry name" value="RNA_pol_I_A49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4367194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="445" mass="48993" checksum="CB447E200BA11C65" modified="2023-11-08" version="1">MSDKKRKRHGENGERPKKKAAIAPTGNVQVELLENKEVLGPLLAITPGLSFPSKTAFKPYKHTKILPQGETTHLLLQSSEHARLDYLAQEEQDGSSESQLKDYVGVFDPATNKLQLVPVKRVVVRSTLRSETEEMREEQAALEAQQNTMTAKRYALAAEFGSKKSRKALEERTLNQIRSGNADDPAAIRNESVAENVLHNMAPSLGAMPSKEELAAAVDSSKPRPTPNLAAEYPGDVYTIDTVVGGELMTMLGVKDWVDASLAGQGIDVSSKYVARRIVKLAKNKQIQKLKVLRFILLCINFNSALMGGGGGSRPRKIPPKGKLEALMGDEVPAGCVMAIRRKFASETGDMPRWNIDNLITHIAAAALIVDDHEVDVNDLREDLKLENKDVRQYFMELGCKVSAPTQADMTKWKLVKAESASHFIAKLKLPLSFPRVGGPQKKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4U0</accession>
    <name>A0A9W8Y4U0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007713</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4U0"/>
    <dbReference type="OrthoDB" id="3798031at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="49"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="231"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="384"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="486"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="826" mass="94670" checksum="33148CDAEC03FBCF" modified="2023-11-08" version="1">MDDSERGAVSDTDPTSSIGSREIEKKKSQELVNALQAKVEENHTLGATVAHLQDELQARDAQYRALEKRYAVQRSQRIKDKETVDEAYEQREAMQVRCADLEEQHSNMSAQSDKTKAEHRAQLQIKDQKLSKLKNHHECAVAVLETVHKEELARKAAATSEALALIRKKDEQLEQTIREGAKDRRDIEAKLGEEKRELIKDAKINRSVWLEKKAFLTEQMERMDDDFRDSLQAQTDEIKRLTECNRRLQAENILLHNMTQGRPISAQEVQEATEVLQQMREELRLTRRREQIANEKIENTLKSMQQQRDVFDSELKKEQEAQVLANGLQKTVAELEEKLKRRNDLLWELKMPTEQLERSQPANHLLLAAATHEANALYKATFENDTMRAELRQAQKEKMEQRSELAKLDAESFELTAQLEAVEKDLNVTKDENKVLRNTRATSDRQSKLLLIALEQGGYLTGNDRAIITQDLVALTRDNQALVTREAVLHDELNSAREQIQVAKDKCDSEVHKIRHWQNYWFSAYHDEAVGKTARLYEEIRELNKELGREVHVEEHLERNVVSERAILRYACLYTLQGVDTSLIPAEYYEPGFVGCELGPIEATYDALRVLRPLGWVPLHRLGKVYLAPMYKPFTEEDAVYRLQAQEQEAHRTKIAFPAPKSMLNAKVFVEERADNITEAQKAMPLIFPNAPLPSIPVRTQPVAFKQHIAPSTPADANAKAAKGLAKQAAPLKAKPQAARRLAPVPAYEAPQAPPYRSFWQKKYDFTREQYNELDEDIKVDWINATIAPEDREVVASRALRLGDGLEMLWKIASDQHDVEGTAQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y528</accession>
    <name>A0A9W8Y528_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Clathrin associated protein complex medium subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">APM4</name>
        <name evidence="9" type="ORF">N0V83_007380</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y528"/>
    <dbReference type="OrthoDB" id="10259133at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09251">
        <property type="entry name" value="AP-2_Mu2_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14836">
        <property type="entry name" value="AP2_Mu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000002">
        <property type="entry name" value="AP-2 complex subunit mu, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043532">
        <property type="entry name" value="AP2_Mu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043512">
        <property type="entry name" value="Mu2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0168">Coated pit</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="8">
        <location>
            <begin position="168"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="438"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4366852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="584" mass="65379" checksum="78FD828138AD1CD5" modified="2023-11-08" version="1">MLSGILIFNQKGENLIFRAFRNDCRPRLADVFRIQVISNAQVRSPILTLGSTTFSHVKHENIYLVAVTKSNANAALVFEFLYRLVGLGKAYFGKFDEEAVKNNFVLVYELLDEILDFGYPQNTETDTLKMYITTEGVKSERAMEDSSKITMQATGALSWRRADIKYRKNEAFVDVIEDVNLLMSATGTVLRADVNGQIIMRAYLSGTPECKFGLNDRLTLGEDHLQQPSGNKAGAKATRAAAGSVTLEDCQFHQCVKLGKFDADRIISFVPPDGEFELMRYRATENVNLPFKVHAIVNEVGKTKVEYSIAIRANYGSKLFATNVVVRIPTPLNTARITERTSQGKAKYEPEHNNIVWKIPRFTGQSEFVLSAEASLTSMTNQKAWSRPPLNLSFSLLMFTSSGLLVRYLKVFEKSNYSSVKWVRYMTRAGNYEISVLVQAAAELVDVLENEAEEACVELTLEDGIAELETEDEEAKAELELEDDTPELEVEVEETWLELAEDDELLKLEAEEVTATEAEEDDTGVKLEIEDEAVENALAALEISVEEPREDATEELLLDATELLEDAEDCAEVLVEEMAKELVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y549</accession>
    <name>A0A9W8Y549_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_006360</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y549"/>
    <dbReference type="OrthoDB" id="508119at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF34">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="326" mass="37246" checksum="F96980CAFBEBE764" modified="2023-11-08" version="1">MVPIYISELAPPAVRGRLVGIYELGWQAGGLVGFWINYGLQETMAPSHKQWIIPFAVQLIPAGLLLLGAIWMRESFRWRFTKGQREQAMKDLCWIRNLPADHPYILEEVEAIDAQIEHDRLHVGPGFWKPFIALKQPDVLKRFFLGSMLFLWQNGSGINAINYYSPTVFSSLGVTGTNTSFLTTGIFGVVKTVLTFVWLFFLIDRLGRTKLLMFGALGGSLCMWYIAAYIKITDPTKNKNTSGDLSSGGISAIFFFYLWTVRNCLYFLIPETKGIPLERMDELFEIKPVFKAHKVMIGRLKEHELEHQDLTMRQEKEAVDERTEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5C4</accession>
    <name>A0A9W8Y5C4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Lysophospholipid acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ale1</name>
        <name evidence="8" type="ORF">N0V83_007532</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5C4"/>
    <dbReference type="OrthoDB" id="286734at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030258">
        <property type="term" value="P:lipid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049941">
        <property type="entry name" value="LPLAT_7/PORCN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906:SF4">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906">
        <property type="entry name" value="PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="376"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="422"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4367002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="582" mass="66135" checksum="1052412A124C3298" modified="2023-11-08" version="1">MLPFINAPFEYVAGALGNSADELKLVFSFYLSYPLAAALKRLPDREPWKKNLFIISVSMFYLVGLFDLWSGLRTILISAIGAYAISSQINSPYMPWIGFVFLMGHMSVNHIYRQAVNDPSVFDITGAQMVLVMKLTAFCWNVQDGRLPESELSEFQKEHAIRTMPGLLDYAGYVFFFPAIMAGPAFDYCDYSQYISTTMFALPPGTDASQAPPTRKQRKIPRSGTPAAIKGVYGTLWLIAFLKFSGWYYPAFFLGSEYMKYGFFRRIWQLYMLGVTTRMKYYAVWSLSEGACILSGIGYNGIDPATNRAKWDRLTNIKPLEIETAQNARAYLGFWNINTNHWLRNYMYLRVTPKGQKPGFRATLATFVTSAFWHGFYPGYYLAFILASFVQTVAKNGRRMIRPLFMTPDGNTPLSSKRYYDIFTLLLTQVVFAFTVAPFILLGFSDTLKVWARVYFYTLIGVAASFAVFSKSLPIRKEITQLQAARTPAPSSITPVDETLVEKTAREAVKRDRLQNNNSQENYAGQRKVPILGIADDPEAEIDEIVREVKAEIEQRRRRGSALQGFDIKKAVQEKLKEFKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5C9</accession>
    <name>A0A9W8Y5C9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">L-lactate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_006716</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5C9"/>
    <dbReference type="OrthoDB" id="1925334at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02922">
        <property type="entry name" value="FCB2_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037458">
        <property type="entry name" value="L-MDH/L-LDH_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF104">
        <property type="entry name" value="CYTOCHROME B2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00557">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="8">
        <location>
            <begin position="2"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="9">
        <location>
            <begin position="107"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4368359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="500" mass="54839" checksum="DCDCE7164BBE4011" modified="2023-11-08" version="1">MSKTVDGAEVAKHSTKKSCWIVLDSKAYDVTSFLSDHPGGAPIILRNAGTDATEEFKKYHPLSYLNDYLPADALVGSVDPNTFGQLRQPKEEKAAPASETDDSKEVPHVSLCVAATDFEKAAKAVLPHKSYIYASGSANNGLALKGNIDDWGRISFRPRVMRNVGDVDTQRKIFGHSSMYPFYISPMGTMGAIHPEAEPEMVRGAVRKGAHVVVSTASSKTSEQIMQSWIDEQARLNNGSPTVLFYQYYMPVDRKKAIALMRIVKKCGYKGLWITVDTPVLGKRTADRYLQAEEALAVGIEEESTAEWEAGGDNDFAPAMGGRPVQGQLSPHTTWEDLEWIRKEWEGPIVLKGVQCAEDAKLAMEYGCEGILLSNHGGRQLHTAPSALMTLLEIRTYCPEVLGKLEIFLDGGLRDGNDVLKALCLGATAVGVGRPFLYALGAYGTKGVERCVDVLAEELQTGMRLLGITSLDQCRPEMVNASRLLNEMWRPEPLNVKSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5X2</accession>
    <name>A0A9W8Y5X2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007459</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5X2"/>
    <dbReference type="OrthoDB" id="3672634at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="60"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="278" mass="31328" checksum="048E09F75A728901" modified="2023-11-08" version="1">MPRDKDQRKPRGDPLAGWKKENLAAPKHQKQQVQDFPQSIYDDVGPVPAPVAKRVTEARKSILKRVPPQQQGKVQEPLGGGGQSEDLAEPKRLKDDEQGMCPDGDTRWPPPKSTHSKPLHLLLDEKGSKRIYYHLIFKNNAPEAKTWKQEIKSKAQFYHKQKFPPAPPITEAGKSAQQIQQEMEQLEIDNKGKARSAIITAKMQRKLRELSSGPRGTIWLLEEENIDVWRCIEIVQKASPPHNAVNLGATTEKFEVFGVGLKGEKDHVLLVSQKVTVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y606</accession>
    <name>A0A9W8Y606_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006812</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y606"/>
    <dbReference type="OrthoDB" id="5413892at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4368455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="232" mass="26744" checksum="995A6F9E168CCD19" modified="2023-11-08" version="1">MNAFVCSMNSAGDDEDGLEPLYAYDTNEKIQDFPKTRADFDQLPGVEVKRILEAIEAPTDGTAEERNKRLKIEVGYYWAPTNYSAETNTLIRELNETRNEPLGYEARLEQEDSRDQENCPVCNLWGKNDKLMPLAAYDTGKDVLNFPETRADLLKLPDEEVERILKTIRAPTDGDKEQRLKCLKDRVGFDRIEFPWTQEDEERLNEKMDTTALEEVHRTMCACLRKARAQVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6P1</accession>
    <name>A0A9W8Y6P1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_006152</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6P1"/>
    <dbReference type="OrthoDB" id="3922703at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040933578" evidence="3">
        <location>
            <begin position="18"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="288"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4369069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="401" mass="44500" checksum="76D56ADA4A4BBB2B" modified="2023-11-08" version="1">MIYPVAVILSLSATISALPQFTNPPRPSNTPATPNDTTTLPVGAEFQQPEEFRISSLNMHMMSEGTGIPGNPPWPDNKRFDTTLDFQVVVPNIDWNRTSTSPATYTHDCHWSWKNGSFPEKYLVKCKGGSPLGLDAIWFQMFEYTGLGPRRPDTGWNLRGDVVITANDPQEPSSYLTCLQGAPYDGLRCTIKGMMSVDNELIVTATYTTEPPRKRGLHTTPQSASWPTTSLAPSLSTTQTPQTSIENTATTSIGPTHSPNNNVLHAPPPPKANLQPNTFANLSTAAKAGIILGITIFFLSVFLILFEFGYLRRKRRERALQRAIEEVERGGGLEMRKRIVESNEMSESKENMVLESRVEIVVDDGGEESDGDGDSVWDTGMEDDDEMWRGRQAMSLPRREY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6T9</accession>
    <name>A0A9W8Y6T9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6T9"/>
    <dbReference type="OrthoDB" id="3358869at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042332">
        <property type="entry name" value="Hsk3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013183">
        <property type="entry name" value="Hsk3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28289">
        <property type="entry name" value="DASH COMPLEX SUBUNIT HSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28289:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT HSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08227">
        <property type="entry name" value="DASH_Hsk3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="60" mass="6215" checksum="B884538E9F12CCA4" modified="2023-11-08" version="1">MADLESLMRMTAGQAQDMRGLGGYAGAMFMAASKVLGEETVKGGGSSDDKAKEDGREGDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y703</accession>
    <name>A0A9W8Y703_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">NAD-dependent histone deacetylase sir2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SIR2</name>
        <name evidence="10" type="ORF">N0V83_006850</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y703"/>
    <dbReference type="OrthoDB" id="420264at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046970">
        <property type="term" value="F:histone H4K16 deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF9">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACETYLASE SIRTUIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="9">
        <location>
            <begin position="154"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="325"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="327"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4367269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="499" mass="55912" checksum="9D951F128EEA6C4A" modified="2023-11-08" version="1">MAAHPDEGPAVVQEAARAPAPKPALGVKAEVKTAAKPEARGDDQDEHDDDEQGDVWESASLYEEILDEVEAFEYSNDGLETCTADEARRLRQRLHEVGASQFVMENITSDKITARKLCTAFGVRIPKFLENAPDEHFYQLLGLAINRELNKRPRLQQYKTIDDAAKLLRERKNIMVITGAGISTSLGIPDFRSKNTGFYSRLLQMGYDEPEQVFDIRNFDEDPRTFYALAGDIIPDLEKWTPTHEFIRLLQDKDKLLTNYTQNIDNVEAHAGIRKEKLIQCHGSWAMATCRKCKYKVPGEDIFDSVRAQKPAECKRCIEEIAAQQPGKKRKRASNGNGSRKKRSSDEDSESDGAYDIPQPGIMKPDITFFGEALPNDFFDRLKDVDKDKVDLVIVMGTSMKVAPVSEIPNFLPRDVPQIYISRDPIHHINFDINLLGDCDVVVAELAHRAGWVLNHKMIPEGQTAEVGTVDAVEHTFSVKVQSSVQTTTNGAASNEPRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y724</accession>
    <name>A0A9W8Y724_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7580 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006014</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y724"/>
    <dbReference type="OrthoDB" id="5331891at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056002">
        <property type="entry name" value="DUF7580"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35186">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35186:SF4">
        <property type="entry name" value="PRION-INHIBITION AND PROPAGATION HELO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24476">
        <property type="entry name" value="DUF7580"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040839658" description="DUF7580 domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7580" evidence="2">
        <location>
            <begin position="205"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="605" mass="68243" checksum="CE9CB592D00A87F3" modified="2023-11-08" version="1">MSGLEVAGLVFGVLPILIEAVKAYSTVSDSLHTFRHWSREVKSISLQLKVQNGIFLNECRLLLRLVEDERAAEIMLEDATDRRWTSKELNDKMNIVLNDNFELCCNIIEETKDVACDMMEEMKRFDALGEQKHKNEKLRVAIKRLRGAIAITFDKSKYEKCLTSLRDRNGDLSTLRLQITAFQQQTTRAAGTLVKHKPLPDRFKSIQDASQKLHEALCGAWCCDNLAHRDYAKLCLDAEAKTEVRLDLAISCHESSNDGQERQPREPPIWLYVQSMSMTTTECVSAASGLMTEMRNSLPAELLVSSSWNPLKKKASSELTQPGSCTKKRKKTRHVHFNETTVAATPPLMTNPGIPEFNLCQTTNICRYLKSSYRACARNCVGYLETPQMYKHMFFAKSNASKDVEYTTIYSISDIMSQEADDFLSVEDQLKLAHKMALATLQFSGTPWMSERWRLAQMSYFGNRHSFDEAALKTLHLSSQISSPTQQNTIAMDGIEHPETGVSDEIRYGINNLPLFFLGVALLEIAHWKPLEEKMIARDANDQVYAARRLASGRAPLGPEYQKIAQKCLQCNFGFGTKLNSTSLQAAVYNDVVCELAGMIEKLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7I3</accession>
    <name>A0A9W8Y7I3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">SUB2</name>
        <name evidence="19" type="ORF">N0V83_006272</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DEAD box helicase family. DECD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7I3"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17950">
        <property type="entry name" value="DEADc_DDX39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000809">
        <property type="entry name" value="ATP-dependent RNA helicase SUB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000111">
        <property type="entry name" value="DEAD-box ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="18">
        <location>
            <begin position="52"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="83"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="272"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="14">
        <location>
            <begin position="52"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ4369187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="438" mass="49234" checksum="B361F9B28BEC6CA5" modified="2023-11-08" version="1">MSHEEDLIDYSDEELETKEAPANGSAAPANGGPAAVDAAGDKKGSYVGIHSTGFRDFLLKDTLVRAITDCGFEHPSEVQQVTIPQAILGNDVICQAKSGLGKTAVFVLATLQQMDDKPEPGVATILVMCHTRELAYQIRNEYNRFAKFLPDVKVGVFYGGTPVQKDVELLSNKETHPHIIVGTPGRINALVRDKHLRLSGLKHFVLDECDKMLDQPDMRNDVQAIFRATPAHKQVMMFSATLAKEIRLVCKKFMQNPLEIYVDDEKKLTLHGLQQYYMKLDEREKNRKLNDLLDSLEFNQVIIFVRSTLRCTELDKLLRECNFPSTAVHSGIGQEERIKRYKEFKEFQTRICVATDVFGRGIDVERINVAINYDMPDKADAYLHRVGRAGRFGTKGLSISFVSSPDDEAVLKSIEERFQVSLPEFPEDGINSATYMDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7Q2</accession>
    <name>A0A9W8Y7Q2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">UV-damaged DNA-binding protein rad7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">RAD7_1</name>
        <name evidence="1" type="ORF">N0V83_006329</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7Q2"/>
    <dbReference type="OrthoDB" id="1924287at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF234">
        <property type="entry name" value="RNI-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4369244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="304" mass="34743" checksum="F69B0E8BB5867633" modified="2023-11-08" version="1">MTSQDLKSFKVEWLDAAFDDETVEALTTFCPNLERLKLERCKRIREDSIDAIARLQHLKHLTLRFYEPVRHEKLIHLITSVGAQLRTLCLEHFLDNTVDPTDDVLETIHKTCRHLSKLRFTENNECSDAGYVGLFSNWENPPLRYIDLNSTRDMDNTNPDGPEDFPIGLGSDGFREMMSHSGERLEYLDISSCRHISHATLAEIFDGTRQYRHLREINLSFCPVVDTHIIAGVFRSCPMLKKVVTFGCFQVADVLVPKGIVLIGAPKAQDQIEQFGDVVFDYQKDTEKERDGMRAMGRIVPVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7V2</accession>
    <name>A0A9W8Y7V2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">YTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_005052</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7V2"/>
    <dbReference type="OrthoDB" id="306690at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990247">
        <property type="term" value="F:N6-methyladenosine-containing RNA reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057720">
        <property type="entry name" value="RRM_YTH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF3">
        <property type="entry name" value="YTH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25701">
        <property type="entry name" value="RRM_YTH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="317"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="YTH" evidence="4">
        <location>
            <begin position="451"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="26"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="393"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="571"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="571"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4370531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="680" mass="73618" checksum="0E57A2501772FFCD" modified="2023-11-08" version="1">MAFVWVQQWVPLPIVYEQQMGDLPSGTRPQALDNATTGFARDGNPSVTSPYKSPENNAPFYSQLSAISQGQLRPDFRSPTHPTAQYQSQEHGASPLNMGSMIGALPEYASFEDASVVPQSIPRSLSGASTSAVAYQLGQNLQMQTHGSGNLPTHTSYGPGFAPGSYQQNFMPSQSSQHSAYPPFGANQPRLTNATSMQPPYQNYPQASQYMYYTPYGAQGQFVPGFAGQTAQGHAMYGRRPSLTNASAGMMGQSADFPHIEGNFLGARVAPGNPQGEANSFGSAYGAAFPQASGMPRSGPVSSIPRGPPRKPKQSGHALWVGNLPPGTTVVALKDHFSREATKDIESLFLISKSNCAFVNYRTEASCTAAMHRFHDSRFNGVRLVCRLRRSSAPASGVPTGPSAMVGNQQQAVSRPETPQQDSVTDGVQGDAESPIHKKTEDIRSTADTASKYFIVKSLTLQDLELSVRNGIWATQSHNEEVLNKAFRSTENVYLIFSANKSGEYFGYARMASPILEDGSKFISSAPKTENIIDAPDVPKSIPTPATEWAPRGKIIDDSARGTIFWEAEMSETEGEGEKEETEEAPQADVDTSVTAQSWGKPFNIEWVSTNRLPFYRTRGLRNPWNANREVKIARDGTELEPSVGERLIQMFHRLGPSTAGAPMMAGPALGGPVAQMRPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y829</accession>
    <name>A0A9W8Y829_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone deacetylase</fullName>
            <ecNumber evidence="3">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HDA1_2</name>
        <name evidence="14" type="ORF">N0V83_005546</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y829"/>
    <dbReference type="OrthoDB" id="424012at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000005">
        <property type="entry name" value="histone deacetylase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019154">
        <property type="entry name" value="Arb2-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017321">
        <property type="entry name" value="Hist_deAcase_II_yeast"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF5">
        <property type="entry name" value="HISTONE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09757">
        <property type="entry name" value="Arb2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037919">
        <property type="entry name" value="HDAC_II_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="12">
        <location>
            <begin position="141"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Arb2-like" evidence="13">
        <location>
            <begin position="505"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="65"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF09757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4369782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="646" mass="72700" checksum="704F5E3206A6D4D9" modified="2023-11-08" version="1">MDDEDYPMEEDTIMASTETNGDSAPDTVAPSEIFGHLKSPPLPKNEQLPFHSPSKITSFLQASNYQSYPGSHRTTKSPSGWSENLTRPTTARRTSPQVRIPPKARASVLPYASSKTGLVYDPRMRFHAELPDMSINPDDIHPEDPRRIHSIFEEIKQAGLVGSSTSEEGAEEAHCWRIAIRPATRPEILLIHTEEHYTFVESLQRMPAEQLRYEAERLDSIYFNHSTYECAKLAAGGAIEACKAVVEGSVRNAIAIIRPPGHHAESTQPSGFCIFNNVPIATRVCQNAYPETCRKVLILDWDVHHGNGIQHAFYDDPNVLYISLHVYKDGNFYPNLPDGNLDYCGEGPGEGKNVNIPWAEHGMGDAEYLYAFQEVVMPIATEFDPDLVIISAGFDAAEGDLLGGCFVTPACYGHMTHMLMRLAKGKLVVCLEGGYNLRSIARSALAVTRVLMLEPPDRLREDLAAPRDSAVVTVQQVKRQHSKYWKCLYPKQLDKADPGYKDTYRLHEIIREWQSQRLSQEHLMTPLPLQINKAGLSQTFEHNVIATPNFTEAHPLLVIFHDPPSFQDHEDPVTGRRELHNTWLTDVTKRYIDWAISNGFQVIDVNIPRIVAVEDADIGYLRSDDSTTRAQQTRELAGYLWENYIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8A1</accession>
    <name>A0A9W8Y8A1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005308</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8A1"/>
    <dbReference type="OrthoDB" id="3045089at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054464">
        <property type="entry name" value="ULD_fung"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47372">
        <property type="entry name" value="DAUER UP-REGULATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47372:SF11">
        <property type="entry name" value="RE19971P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22893">
        <property type="entry name" value="ULD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="554"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="414"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="504"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="336"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="780" mass="86324" checksum="262C9BD5389053F1" modified="2023-11-08" version="1">MPKGITVNHNTGEYYTDEEDSSGVQVSSGSQGDYTDESEEEDDAEDDEWDSCGDYIDEIQPSDSASRPQVKRHVVPAPAAPAPRAPRPKVSHRASSRHAVPVSDPPSRQRSHSRTTLDPEHRPRRRRRQSVHADSLEDQDDYPYAPSARTPAPPGHPSQGWGHIPQAPAPNAYAPSLMSDPRFHGYPGGAQSPAGQLVPFGHDPYYAPQQNPFAPNGQNPFTGPPPPGRDPNGYFPEYQDPRQGRRGHRNSMPPPPQGEMMPFSPMGYYPPYGAPYGMPGMPMYPPYGHPQHPSPPPQHPPSKRPTPKPQHHEEEPQAAPPPPPPPPPPSHTPGAMQMQMQPLQHMQMPMMPPPPPPPDPKEDGMLTKLEKLLLNKAEDDEKAAEAARKAAEEAKFARLENLLISQQEARIEKDKAKKQAAEDAAKAAADAKKKGDEDKLAKLEKLILAQKDEQLKREAAADAARAAEKAAADAEAAKVAADKKAAAESAKLLLDAAQKAREEAEKKAAADAEETKKAHEKALAEAKAAAEELEKGKKAAEEEAAKLKPSDAPKAPIKFKDAVGRKFSFPWHLCKTWKVCNIPKLFFDSNANMTIRAWKNSSNKPSSTLMLLAPTSTKAIMTSLVPTVRSSCHKYGKPWYSLTGPSPCTCGPCLSLHQRRSHRETSLSHLRHQTQQHSWPRICPWARARAPKGTRVESVSPVTTLMLSKCLLCLPAPRPSRRWHASSPPLPWCLWWWHASSSTTACSRRRGRWWWWWLWRIVITQEVGPESVLQVGCWRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8C3</accession>
    <name>A0A9W8Y8C3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005114</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8C3"/>
    <dbReference type="OrthoDB" id="3680743at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040720389" evidence="2">
        <location>
            <begin position="26"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="314" mass="35111" checksum="12EDA0FDA5D7B914" modified="2023-11-08" version="1">MIVSMGHLATIFLTFFALFARPAFAATTQTPDCQIIGDADVYGLGVRLSFYLQWVASIVQLFFCPGTSETTRSAAVITVLAVFINILRNLNSETLVAVEWSMLYSVLTFLLSWNIPWTKAARCKLDRTGGSYFVLFFILAVYQIMCPYIIYHAWDYGPQPGCSAKFIFWTAIDAYSKGWIIFLKVSWTLAPFLPGTLYLLFAIYALLKWLTPWNILGTGIMKLLSKMEASFEDDEEKEKTSDVGIAWFAKLFAISCGAIGIGFLEATIKENNILFPDTHLTGSGQLLPLLIGTFTLVVTILDAIRAYPGSWNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8G6</accession>
    <name>A0A9W8Y8G6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Protein fmp32, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">FMP32</name>
        <name evidence="12" type="ORF">N0V83_005174</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CCDC90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8G6"/>
    <dbReference type="OrthoDB" id="889336at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000018">
        <property type="entry name" value="Mitochondrial protein FMP32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024461">
        <property type="entry name" value="CCDC90-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360:SF1">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07798">
        <property type="entry name" value="CCDC90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="178"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4370653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="273" mass="31218" checksum="DC717B1E46ADAB86" modified="2023-11-08" version="1">MVAPSRHATAIPRFLLPQLSWSAKATRPAALGALEARRHRSYAASDRRAMVSKPSLLQAQTIRQWKRSSPHYADGYASALEFRRNFSATAPQSKDHHFDTLKFVQRLKDEGFTEEQAEGMMKVLGDVIEESIQNLTRTMVLREDQEKATYTQKVDFAKLRSELMTADSTESSLTRASHERLTNELAKLNSRLRDEIQRTQASVRLDLNLEKGRIREEANVQELKLKETETRIEQETAQLRERLEAVKFSTLQWLMGVCTGTAALMLGVWRLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8L7</accession>
    <name>A0A9W8Y8L7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_005768</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4370004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8L7"/>
    <dbReference type="OrthoDB" id="3792666at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4370004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="327" mass="36884" checksum="BC50FCFB44913067" modified="2023-11-08" version="1">MPPTARIPTVEFRSLLSPPVRVPLRAIRSLYRPQLPPIVHHVPRPSQCLAQCRSLHLYKTAKAKTALGMHKVLTFSPYKVTEPTASPHKVQLVEDEHDPITAGKDITLQVLYDNHVKPGHMLYFTKTPQKAIAKRAENLEEDAIPPADRQFALVKAHTRFVHIAKSQKGAQFGALKIVIVSLMNPVHYFKLALDRAYQFILAGSPVEFRLRLCSSPKLSSKQDRHKPYDPAVWPWLHDRFPHLRPDFVLRGMPEGTVYLVEPVSDGRRVQWVMARPVDQMPKVDLNARLVRVKTTVGNLVRQGKLRQVPKGLKEGLEAPGVGRGWGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8V6</accession>
    <name>A0A9W8Y8V6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8021 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005015</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8V6"/>
    <dbReference type="OrthoDB" id="3504677at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058334">
        <property type="entry name" value="DUF8021"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26061">
        <property type="entry name" value="DUF8021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040976411" description="DUF8021 domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="DUF8021" evidence="2">
        <location>
            <begin position="150"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="267" mass="28834" checksum="DA0D8CD84FEF2C5D" modified="2023-11-08" version="1">MRNFIPAILGALVVQVSAECTRDTLKAVTDELLAAQTAGQTSFNSLASTVAYSENRKKVDIKSGILSKALKVDHVRAQHDVPQCAAFSEFIVTNSAAPYVITMQLRLDNATSKVSQIDSIVTTKGDWLFNVTGTYYWASQEKWDPIPADKRDSRAVIQAAGDAYCDLFNNKSVKVPWGSPCARLEGGSYTGKGQATDRCDVGVPSGVKLVNRQYVVDEEYGTVDIMMDFGGTPGGTGGLPDSHEFRVESGKLRYVHTLSSCGGKSCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8X0</accession>
    <name>A0A9W8Y8X0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Vacuolar protein sorting-associated protein 68</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">VPS68</name>
        <name evidence="7" type="ORF">N0V83_005453</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0220 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8X0"/>
    <dbReference type="OrthoDB" id="268928at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007919">
        <property type="entry name" value="UPF0220"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13180">
        <property type="entry name" value="SMALL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05255">
        <property type="entry name" value="UPF0220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4369691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="173" mass="18890" checksum="692099F02AA4758C" modified="2023-11-08" version="1">MEDNRLFRFPKPAWMNSANTRTAGVYIAGALFALGFYTLIDISVWSKSVRNPSDPPVHITFVDWIPGICSALGMLVINSIDKSRLSADSFSYSGNGVAWKARLVLFLGFALLAGGLAGSAVVLAMKYIVPEYTWPTIWMGVGNVVANGLVMLSSAVLWIAQNMEDEYTYNLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8X1</accession>
    <name>A0A9W8Y8X1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycogen debranching enzyme</fullName>
            <ecNumber evidence="5">2.4.1.25</ecNumber>
            <ecNumber evidence="6">3.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Glycogen debrancher</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">GDB1</name>
        <name evidence="21" type="ORF">N0V83_005045</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Hydrolysis of (1-&gt;6)-alpha-D-glucosidic branch linkages in glycogen phosphorylase limit dextrin.</text>
            <dbReference type="EC" id="3.2.1.33"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1-&gt;4)-alpha-D-glucan.</text>
            <dbReference type="EC" id="2.4.1.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the glycogen debranching enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.25" evidence="5"/>
    <dbReference type="EC" id="3.2.1.33" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10248904at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004134">
        <property type="term" value="F:4-alpha-glucanotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004135">
        <property type="term" value="F:amylo-alpha-1,6-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11327">
        <property type="entry name" value="AmyAc_Glg_debranch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000039">
        <property type="entry name" value="Glycogen debranching enzyme Gdb1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000242">
        <property type="entry name" value="Glycogen debranching enzyme Gdb1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000333">
        <property type="entry name" value="Glycogen debranching enzyme Gdb1, putative (AFU_orthologue AFUA_1G02140)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010401">
        <property type="entry name" value="AGL/Gdb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032788">
        <property type="entry name" value="AGL_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029436">
        <property type="entry name" value="AGL_euk_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032792">
        <property type="entry name" value="AGL_glucanoTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032790">
        <property type="entry name" value="GDE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10569">
        <property type="entry name" value="GLYCOGEN DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10569:SF2">
        <property type="entry name" value="GLYCOGEN DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06202">
        <property type="entry name" value="GDE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14701">
        <property type="entry name" value="hDGE_amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14702">
        <property type="entry name" value="hGDE_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14699">
        <property type="entry name" value="hGDE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Eukaryotic glycogen debranching enzyme N-terminal" evidence="18">
        <location>
            <begin position="41"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Glycogen debranching enzyme glucanotransferase" evidence="19">
        <location>
            <begin position="139"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="Glycogen debranching enzyme central" evidence="20">
        <location>
            <begin position="748"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="domain" description="Glycogen debranching enzyme C-terminal" evidence="17">
        <location>
            <begin position="1075"/>
            <end position="1509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF06202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF14699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF14701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF14702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ4370524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1524" mass="171990" checksum="ADD0A09933A2F8DB" modified="2023-11-08" version="1">MSAPRQKPQAAYLLPLTDAGAPDVPGMYINLPPPTEPAYVVRFQIQGTSSICRHGSLWVNIPSKGDKFERSRYREYKLRPDFNGITEIDVPIHEANAYSFYTTYTPLPQWSFTDVASPEPTRTPTYYIDVCPRLTMHGQQLPVEAVIVFSCLSKFMGNYPTDWDNHLRGISERGYNMVHFTPLMERGSSNSPYSIYDQLAFDKAVFTNGEQDISRMVEKMQSEFNLLAMTDVVWNHTANNSKWLEEHPEAGYNVDTAPWLQPALDLDTALLKFGKELGARGLPTTFNSVDDLLKVMEGIKTHVLGVCKLWEYYVIDVERDTNAVVQAWVAGQAKFLEDSFSEAGLRGLDAVKDWPLKKKADWLIDNALSGGDRMGERYRRRIDPAVGAGLLCALFGRFDSRTSSTPDERAAHGALTRILHECNLPFYREYDGDSAEILEQLFNRIKYVRIDDHGPKLGEVNDANPLIEPYFTRLPLNETTKKHNPEALALVNNGWVWAADAMRDNAGPKSRAYLRREVIVWGDCVKLRYGDGPQDNPYLWEHMANYTRLMAKYFQGFRIDNCHSTPIHLAEYMLDQARTVNPNIFVCAELFSGSEEMDFKFVMQLGISALIREGMQAWSTQELSRLVHRHGGVPIGSFETDEVLNAEHAAGGSSSVKANTIIKKIKRSPVHALFMDCTHDNETPAQKRDARDTLPSAALVAMCSCATGSVFGFDEVYPELIELVHETRLYSSSNSTGDTIKAQAGEGGIGGIRKIINGIHVKMGKEEYDETYIHHDQEYITVHRVNPHTRKGYFLIAHTAFPGYGSSNGGFGQTKLPGTKAKLIGSWNLEVDNSPETRARIRGDKTTLRGLPSRTNELQGVVIGSNGDDATIDIPEKFPPGSIALFETWVPSAEHADGLDKFVTSGARTAFRDVSLTDLNYILYRADAEERDASDNRDGTYNVPGHETLVYAGLQGWWSVLRDIVNDNNLGHPLCNHLREGQWALDYCVGRLERISKKSKYHQIKGPAQWLKKKCDAIRQVPNFLLPRYFALVIQTAYNAAVERAIELMGDNVRNGNHFLKSLALVSVQVTGYMNSASLWPDKSVPSMAAGLPHFAYDWARCWGRDITISARGLYMGTGRYADAKEHIFAFCQCPQTRITRRSCPTAWDLLQETVRRRFSPYDDTWFPHDDKQAYSVSSTIEDVIQEALQRHASGIKFREYDAGPNIDSQMKSEGFNIEIWTDWDTGLIFGGNQFNCGTWMDKMGESEKAGSKGVPGTPRDGAPVEIIGMLYSTVRWCADLYEQGKFKYEGVTLPDNEQTITYKAWADKIKTNFERAFYVPRTPEEDQDYDVNPSIINRRGIYKDLYRSGKEYEDYQLRPNFPVAMTVAPDLFDADHALYALQTADRVLLGPQGMKTLDPSDLNYRGYYINSEDSTDFHTSKGRNYHQGPEWVWPTGFFLRALLKFDLKRRKSPAERIESYQQVTRRLAGCMKAIQESPWAGLTELTQKDGAFCGDSCPTQAWSASCIIDLFQDAREYELEESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y952</accession>
    <name>A0A9W8Y952_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Zf-C3HC-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_004547</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y952"/>
    <dbReference type="OrthoDB" id="2592092at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013909">
        <property type="entry name" value="NuBaID_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012935">
        <property type="entry name" value="NuBaID_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15835">
        <property type="entry name" value="NUCLEAR-INTERACTING PARTNER OF ALK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15835:SF6">
        <property type="entry name" value="ZINC FINGER C3HC-TYPE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08600">
        <property type="entry name" value="NuBaID_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07967">
        <property type="entry name" value="zf-C3HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3HC-type" evidence="7">
        <location>
            <begin position="118"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="NuBaID C-terminal" evidence="8">
        <location>
            <begin position="303"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="28"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="421"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="462"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="491"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4371330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="501" mass="55676" checksum="AECEB052630C0889" modified="2023-11-08" version="1">MASPDSQPALATTKRKFHKLLDNLTASTSTTSLASTLQESNVSSTSLSQPGTPEPPNKRPRSSNLSMERERNVSAGQERIRVLKEQLLTPRREGTIKVVGKKQAPAVTTPRKAPNFQPYNQQQFLDRLRTFADVKKWTTKPDSLSEVEWAKRGWTCDTWNTVACKGGCEKRVAVKLRPRRKDLDGKDIEMSEDLAVDVEDALVENYRELIATGHSEECLWRKRGCQEDIYHIPIGSRNQSKAELLTRYSSFKAMAADLPLLENIIYPDPAIHDVLKSIPPAYFNTGDPESTVSPPSSPTDIVAFAFALFGWSGASESRISLAVCNHCFQRLGLWLSSDTRLKEMSKKLDVPIESLRLNLLESHREHCPWKNAEVQGNASDGPTAHMAAWQSLEFMLLGKQKDKGSAQGFVAGIGGAHSRHNRNVESVDLGSEVTYPRGSMDSGGDERSVREVEEEADSLQQKWKKLKAKLKRTASKKSLRSVKSMRSGKSVGDKEGGKEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y985</accession>
    <name>A0A9W8Y985_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="15">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">SPB4</name>
        <name evidence="19" type="ORF">N0V83_004802</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits. Binds 90S pre-ribosomal particles and dissociates from pre-60S ribosomal particles after processing of 27SB pre-rRNA. Required for the normal formation of 18S rRNA through the processing of pre-rRNAs at sites A0, A1 and A2, and the normal formation of 25S and 5.8S rRNAs through the processing of pre-rRNAs at sites C1 and C2.</text>
    </comment>
    <comment type="function">
        <text evidence="15">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of pre-60S ribosomal complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DEAD box helicase family. DDX55/SPB4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="15"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y985"/>
    <dbReference type="OrthoDB" id="7396459at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17960">
        <property type="entry name" value="DEADc_DDX55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056330">
        <property type="entry name" value="CTT_SPB4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23681">
        <property type="entry name" value="CTT_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 14 19" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 14 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="42"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="297"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="553"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="622"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="553"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="594"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="628"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ4371583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="641" mass="71259" checksum="C63EDE9545F4D22F" modified="2023-11-08" version="1">MAPQAKIDRSFGALTPALSEWILEAVDAMGFVKTTPVQHAAIPMFMKNSDVVVEAVTGSGKTLAFLIPIVERLLRLEAPLKKHHVGAIIISPTRELATQIHAVLCSLLKFHAPSAALLEPEDEDTDMEDADAPPRPTFPPGTLKIVPQLLLGGSVTPAQDLSRFLKTSVNVLIGTPGRLLELLSSPHVHCSQSSFDALVLDEADRLLDLGFKDDLQKILSRLPKQRRTGLFSASVSEAVDQLIRVGLRNPVRIAVKVKARAAANGTTGEAGAMEDKRTPASLQMSYLVLPASHKLPAVKKVLSSLQPQPQKSIIYLATCFSVDYFQHVLPEVLKGYTIVPLHGKHPDKVRRKNFTKFVDSVTPSVLLTTDVAARGLDIPSVDLVIQLDAPSDPKTFIHRCGRAGRAGRKGLAITFLSPGREEDYIEFLRVRQTPISPLTSLDITVTPEDAQAVSNRIRKVVKTDRALFDKAQRGFVSWVRAYSKHTASSIFRVADLDWTELGHAWGLLKLPSMPELKKWEGDRGLGLDMDFSTFAYKDKTREQQRQIEQAEYEATKGEGKKRYVPENKKESAWTQQKDQKATKELRREKKAAKREHDRVLKLTDEEKKEEERLKGMIDQMRKKVAETEAQEAADEFEGFSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9K7</accession>
    <name>A0A9W8Y9K7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Zf-UBP-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_004774</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:protein ubiquitination"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ETP1_RRM"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24007">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24007:SF7">
        <property type="entry name" value="BRCA1-ASSOCIATED PROTEIN"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="SUPFAM" id="SSF57850">
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        </source>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="710" mass="78952" checksum="229A4030E6FA4F18" modified="2023-11-08" version="1">MPAYFFHLTIELYPTRSLPATTFAPPPHTDIFNSELPAHRRASWTSPAPIPRSYESKSKPVPSSSDAAQPAPTRERPAATRRVSQRTRLSFSDHHDSPVPIIKPPKRPGHRRTQSFAHRDWRFDVISILSIDMSQSNGHDIARPRAKSLKHPSHAPNAGGLATKGKFIPSDLKETELGWGVVHLYRDGQETPGLYDEVDGAGKDFREEDCTTLCILAVPSYMMPSDLLGFMGEQTREDVSHFRLIRTSRANKYMVLMKFREAQKAREWRKEWDGKPFNSMEPEYCHVVFVKSINFQNGDSNRDPTSYPDLTNDPFAPAPTQQPTAPLPPATGVNPPIEGPSLASSLTTKPHAPPTPALVELPTCPVCLERMDETTGLLTILCQHVFHCACLEKWRGSGCPVCRYTQNDAFTSHRGSDGDSPDNECSVCGSTQNLWICLICGNTGCGRYDSAHAFAHYEATSHTYAMDVVTQHVWDYAGDGYVHRLIQNKADGKLVDLPASTHQTFNTPGMTGYANDTVPREKLDNMGMEYAYLLTSQLESQRAYFEEQLERAVDKAAKAASSADEASRSVATLSQKLDKLSLQHSEATSIIASLEKESARNAQKASSASELARKLTKQYKEEQTINESLMERIKHLEKTALEADAKVHALEAQKADLEEQNRDLSFFISGQEKLKEMQGNEIMGLHEGEVEGGTVEVGDKGKRRKGKKKT</sequence>
</entry>
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    <name>A0A9W8Y9W9_9PLEO</name>
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        </lineage>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <source>
            <dbReference type="EMBL" id="KAJ4372013.1"/>
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</entry>
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            <authorList>
                <person name="Hill R."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="2">IMI 356814</strain>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <source>
            <dbReference type="EMBL" id="KAJ4371516.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1290" mass="140945" checksum="DCFAB857B62BD77E" modified="2023-11-08" version="1">MAPRGRFASASHDADRSYESSPDPLALSTNENKAKATRNTAPKQPLTATSPSKQNSRLSLSELGFSSPSKSLVMSTPRMGNASPWRIKVTVQAEPGSDEENTESPSVKRITRTKTTTVPLKDPDAQSPAKRPRGRPRKSDVGSAAKPKRNGTPVRRVARSRSRDASMGAVESSAADVDTDVPPKKRRGRPRKSVQPPIEDDETLVVQVSEPQDIPTLEVTPVPAMESTSTSSKKNTRFVSPQAVLPAIDLLAQDDLSTVDTPPLAALGNKFRARKGTPHAKKVVPLESLEDEDSDEHSDVLTPSSEDEEKERGNTIQDPMASRAALQSLHTSSENSSSPSAVNVPIIRTTIDEGDATASPAQAFEDGEDADVHDITNFAFDEGATRMPDDTTVLDSENFSMISVDSLPSNGGLSSPPRPEDHQNAMKPSIGSTLQHEYLMLSADTSNSSVSQTANRPSPNPSHGLPSSVSNNSLRPTPSPRYETPDVDTVVPSVPPAIQPLQTSESKTETLGLGRAVTAGVALQGVLDPSRLTPQPSQKALDDKRDELDDLFRGFSEGTRRELQAGLRLGEQLAQGQTKGQSSPAVSSPIKGKPEVAPREGVFRTHRKYRQSRLLTPEEQDDHIITPDTESVQTNDIQYPSLNTEEVQNSLLSPARSEDEMSWRVDTPPVVVANAERGPLGNMSNEHRHLHRGHGSAVTANTSKPMQHEDYSDIWQEEASRSSNSSETEDVPAKGSPQLQDLFAEDGPVKPARGKLPRTWRRTSANNFQYSDEAESPEHASNPHMATAELASVRHNNGKAKVQDPPIVEAEETEIEDDEESVMSDASDDTGMFFQSNMPNIFNAKRSIELKQKKADKLSLSTLLDQGESLLPESSPPIATKESRSETKTNPFLNTPPRFPGFAASPKKSSPLRQELRGSDFSSDSPSRIADESSLPLPQSSPFHTFVDGETVLSTASDQRQFRVEMEGTTASSIRRVRNEANEYLDAYEPQERSLNEITEVTEPSRTWHKDTSLIPSSPPRRKQNFALSMLSPTRRPVPLFSNHNQESDQMTASRPMHAAESTEEHESDESSAYADTEASTEPTPQARNNTAPTKGPEQPTTGLFSRLTSTLFEALSRPAPLPRHPILSRLTPLPKVQPWTKTHYKALDKLYTTHLKHPALFSPSISPSTPLSETNASLLNTFVATNKHAYVGAVYSAWGYSMVMSEELVVLCAVYMQLLTLDGITEYEKVTKIEIQMGDCAPGQSGEVIKGDDVVRRLATVVLGEDVRRDEKQGLQIDRREALSVVWPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAF6</accession>
    <name>A0A9W8YAF6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HpcH/HpaI aldolase/citrate lyase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_003958</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAF6"/>
    <dbReference type="OrthoDB" id="1773at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000023">
        <property type="entry name" value="CitE, Citrate lyase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005000">
        <property type="entry name" value="Aldolase/citrate-lyase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011206">
        <property type="entry name" value="Citrate_lyase_beta/mcl1/mcl2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32308:SF0">
        <property type="entry name" value="HPCH_HPAI ALDOLASE_CITRATE LYASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32308">
        <property type="entry name" value="LYASE BETA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G13030)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03328">
        <property type="entry name" value="HpcH_HpaI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015582">
        <property type="entry name" value="Cit_lyase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HpcH/HpaI aldolase/citrate lyase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR015582-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR015582-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4372185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="308" mass="33623" checksum="9720251D11ECE564" modified="2023-11-08" version="1">MYVPSSSKKMLEKSFAMHADNVTYDLEDSVAPHMKGEARSNLRDILNKQRPSGIKEMCVRINSVDTGLALDDLTEVLKGEYLDAIMLPKTESAADLHFVTDVIRHVLPERHPSSGQGKKEPLRIIALIESAKAIQNLSEICKASPYLSGLVFAAEDFAKDMSLTRTPALTEFLYARSAIATAARAALLPSTIDLVCTSYKGDAGLKTLEEECLNGKGMGFNGKQCIHPSQIAVCHKAFSPGEKEVEWAARVVIADEKASKAGRGAWTLDGKMIDVPVVKKAQAVLKHAEVCEIDVGAVRERWSDQEPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAJ6</accession>
    <name>A0A9W8YAJ6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP-dependent DNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_004919</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5330228at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18070">
        <property type="entry name" value="DEXQc_SHPRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000059">
        <property type="entry name" value="SNF2 family helicase/ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052583">
        <property type="entry name" value="ATP-helicase/E3_Ub-Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059033">
        <property type="entry name" value="C144_05_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45865:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SHPRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45865">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SHPRH FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26021">
        <property type="entry name" value="Ferritin_C144_05"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="343"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="1151"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="1260"/>
            <end position="1415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="464"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="750"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="750"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4370401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1494" mass="168905" checksum="66116DD5DC6D9537" modified="2023-11-08" version="1">MASLQGRADALSANTYVPVPASRNGPRLSLAAQDAPSYLRAIHSLFAAPTARDGEERTQKRRKLDDGSSAPVTRADFDESKSIVLEKVSLDLNFPTLSQDETDIPDTPSNLFVPVAISLESFHKAEANAFRAVVWNPSTITGIAIVATIAPALLQAISPHLRTAETLATTRNGKKREPVARATFCRCLLIPPPDGHNMYKLEVEIRWRLGMSVVEDPAVRASHMKEDLGLLAHHFRDATSPAKTTWELSDFYDSVYVPPTDLHVSPRIQQSLTDTSLYPFQQRAVDWLLQREGVAFSPSGILESFVESSRPVSFRQTKDATGKLCYISQLRGMVVTNLDGVKTDIMQSLRGGVLAEEMGLGKTVELIALMAHHQRVIPDGDVFDAYTGAHVKPSGATLIITPPSILEQWISELRTHAPQLKVFHYRGLPAPSAPKKDHASATVENLMRYDVVLTTYHVLSREIHHATPPPDRSSRHNKRPERRTSPLVGISWWRVCLDEAQMIESGVSQAARVARIIPRCNAWAVSGTPLRKDVQDLRGLLIFLRCDTIVNNKAVWDCLDKASFIAIFHQLALRHTKDKIRDELRLPPQKRVVITIPFTAIEEQHYTELMRQMCNACGLTLEGNPIEEGRDTRDPYVIERMREWLIRLRQTCLHAHVGRRNRKALGAKHGALRTVHEVLEVMIEQGLGSWRSEAREMILNHVKLGHIKAYAGNFDDRAQRALPYYEEALAEALKYVKMCRDELQAERQKLEKASNAHDLASEAGREESGDEEEAEGESLGRVPVIRKSLRSFLELEHACYFFIATTYHQIKENEKFTEPDSEEFHRLEKLETEWYEKAKVVRRELLKESKSRARQQMARVNSKKPFNQVSKIDDLLGLGGIEARKILEMMDNIGDYMNAQAEQIQIWRMKIVDILLMRLVDDEDDQETTGEEYDDSLKAQDELYVYIMALRSLVADRNAVVHGLHDTLVEHELKAAEKQALKTDDEGNRGHAPELVIEVSNIRRKLQAMLEDGSLKGVVSGIRSMVTALQWRADGGDSRAAAELDVVQKHFSRIQSIATQQAKIITELEKEQEMFRATMNQRLEYYRQFQHISDTVAKFKEDLDVTFDEREFNRIDALRKKNENAVAGLRTKYTYLTHLRLENQQDAKAECIICQDVIELGVLTTCGHKYCKDCINTWWHAHRTCPLCKQRLGSSDFKDISFKPSEMKAQEESHDPVSPTQVSTPGLSPNASIYSDISHSTMKEIKMVDLDGSYGTKIDMIARHLIWIRNNDPGAKSIIFSQFGDFLEVLRDALQNWKIGVSGITDKHGIQRFKSDPAVECFLLDAKSDSSGLNLVNATYVFLCEPLINPAIELQAIARVHRIGQQRATTVFMYLVSETVEEAIYDISVTRRLEHISSASKAKLMEFRSGSATPMIQEKTLDAANSAELQAAPVQQLLRKKGEGEIVQVDDLWRCLFGKPRKQIKPVLEMEVGKHLRAEAVDARRERMSAQHRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAR4</accession>
    <name>A0A9W8YAR4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Selenoprotein O</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_005789</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SELO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4370025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAR4"/>
    <dbReference type="OrthoDB" id="10254721at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070733">
        <property type="term" value="F:AMPylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00692">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003846">
        <property type="entry name" value="SelO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057:SF14">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02696">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4370025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="618" mass="70160" checksum="A496B0D5A242C29A" modified="2023-11-08" version="1">MAHLDNSQASNERRTLQSLPKSNVFTQNLPADAAFATPKDSHSAPRQTLGPRMVKEALYTYVRPDAQGEAELLAVSQRALQDIGLKEEEAETEEFKDVVAGRKILTWDEEKPDEGIYPWAQCYGGYQFGQWAGQLGDGRAISLFETTNPSTGTRYEIQLKGAGRTPYSRFADGRAVLRSSIREFVVSEYLNAIGIPTTRALALTLNKGSKIMRERVEPGAIVTRFAQSWIRFGTFDLQRIRGDRKTLRTLADYTAEHVYGGWDKLPSKLPTGDAKETHSQASTGISKDTIEGDGLEAENRYVRLYRAILRANAQTVAKWQAYGFMNGVLNTDNTSILGLSIDFGPFAFLDTFDPTYTPNHDDHMLRYSYRNQPTIIWWNLVRLGEALGELMGAGAKVDEGVFVEKGVTEEEADELVKRAEGVIDRCGEEYKAVFLAEYKRVMTSRLGLKTQKDSDFEVLMSELLDCLEAFELDFHHAFRRLSAISLEDVDAEDKRRDVAGRFFKENDAPRQEADSRQRIGKWLGKWAERVKEDWGEGKDAERQAAMHTVNPKFVPRSWILDELIERVEKKDEREILRHIMKLSLSPFEENWGWDESEEERFCGDVPKYKGMMQCSCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB47</accession>
    <name>A0A9W8YB47_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA mismatch repair protein S5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_004011</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB47"/>
    <dbReference type="OrthoDB" id="10263226at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032389">
        <property type="term" value="C:MutLalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061982">
        <property type="term" value="P:meiosis I cell cycle process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16926">
        <property type="entry name" value="HATPase_MutL-MLH-PMS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03485">
        <property type="entry name" value="MutL_Trans_hPMS_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000017">
        <property type="entry name" value="PMS1 homolog 1, mismatch repair system component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="MutL/Mlh/PMS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF41">
        <property type="entry name" value="MISMATCH REPAIR PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G05820)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein S5" evidence="4">
        <location>
            <begin position="229"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="472"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="584"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="720"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="758"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="813"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1023"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="376"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="472"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="727"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="758"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="776"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="816"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="839"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1040"/>
            <end position="1052"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4372237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1052" mass="115580" checksum="FB863DB9743F5460" modified="2023-11-08" version="1">MTESAVGALPGIAALPPTTARQIGSGQVLIDPGSVVKELIDNALDARAKSIFVDITTNTIDSIQVRDDGHGIPAEDRALVCRRYCTSKIRDFHDLKDVGGKWLGFRGEALSSMAEMSDTLSVITRVEGEPVAVKLSYGRDGELTSSTGRVSHPVGTTVKVTNLFEHLPVRKQTAIKSSARWLAKIRRLMQAYALARPAIRLRLRVLKAKNEKGDFVYAPKADAKFEDTVLKVIGKDCALQCDWTALETDGFEIHAFLPKSTAIVLKVVNFGAFISIDARPMSNSGKTVKKVVAAVRDALRKSNSLYGGARDPFFCINIICPPQSYDPNIEPAKDDVLFENEGVVIGAVQMLLASYYPVAITDTGDIETPTPAQQPYDHEPEDLPARDQTPMEVYEDAPIGLSQELPQSQPRWRSSMYGIDEDDLDFLQENQPPEIEEEEGLRAAAVSNPWTIARMNAINKPRKSTTIEQLLSPAKSQSEAAFMSSSPAIASTPQKSPQAEPLTPQNLSPKKISPASLDSELRRNIQHLPQPSQEGHLLGLSNGDETMSQNTALSSLLASEAEDSIYVARPGFQHPGLQHASADRFNPILPVTPPPASSAPRRSQRKQQGRSKESLAPRAQMSDESWFGQPMRGSQPSQPTRKQKKVQHQNVPLFASDMPSSPTSPFRTAGESLGNRLCSENNADIRDFLGHNGEGHTRASDRALQGSSFTPINVPFGRASDLAPRSSPFTSINVPSQSNQSRGQRPLFGDRAERCFPRTQQGTSLNSSSRAISAEPQHRSLRVRDQSLLHSDRGTSTEPVDMAAHFRAYAERESSLRANNPRNSPSLGSPDKARRLRRRTTDGLERTKSSKLPLERVPSAYRIQDIVLPFATSLATIVQSSRKLDMGYNSLEWGYPADIACDVFAEPITERKIMHWVIRLDETLHEQFGRMADVDTCCELHEGIQRELDARKEQDGTMDTMEGVIRALTPVPFDNAIRKVADDLSSYPVHQLATQAPRRADDEMSDFDMSQFIDLDAELPGTTSGIVDDGNASVTVSGDSGEEFDDDMLMDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBE7</accession>
    <name>A0A9W8YBE7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GTP:AMP phosphotransferase, mitochondrial</fullName>
            <ecNumber evidence="5">2.7.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Adenylate kinase 3</fullName>
            <shortName evidence="5">AK 3</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">ADK2</name>
        <name evidence="8" type="ORF">N0V83_004161</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a ribonucleoside 5'-triphosphate + AMP = a ribonucleoside 5'-diphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:13749"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="5">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon GTP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent GTP hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the adenylate kinase family. AK3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.10" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBE7"/>
    <dbReference type="OrthoDB" id="439792at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046899">
        <property type="term" value="F:nucleoside triphosphate adenylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006172">
        <property type="term" value="P:ADP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046033">
        <property type="term" value="P:AMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046039">
        <property type="term" value="P:GTP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046041">
        <property type="term" value="P:ITP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03169">
        <property type="entry name" value="Adenylate_kinase_AK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007862">
        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036193">
        <property type="entry name" value="ADK_active_lid_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028586">
        <property type="entry name" value="AK3/Ak4_mitochondrial"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57774">
        <property type="entry name" value="Microbial and mitochondrial ADK, insert 'zinc finger' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Adenylate kinase active site lid" evidence="7">
        <location>
            <begin position="177"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMPbind" evidence="5">
        <location>
            <begin position="40"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="5">
        <location>
            <begin position="176"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="19"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="67"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="140"/>
            <end position="143"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="186"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4372387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="270" mass="29943" checksum="38E1BC5883D320C5" modified="2023-11-08" version="1">MGPIQLSRAARIILVGAPGVGKGTQTERLMKKFPELSSISSGDLLRMNVRERTPLGIQAESKMKSGALVPDAMILRLIVNELTTRGWIRDSALRPYAVYSSSSSVDMAEGSVDSIVIPSALRTSRYTFSDQPSASFILDGFPRTATQAIQLDDIVPINMVVNLNTPSEIIIDRICNRWVHAPSGRVYNTTFNAPKVEGKDDMTGEPLTRRADDDPEVWKARLKSFKENNEPLLEHYDKMGILWTVNGNSSDEISPQLFNEFGKRFGVVEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBI9</accession>
    <name>A0A9W8YBI9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Type I HSP40 co-chaperone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">YDJ1</name>
        <name evidence="10" type="ORF">N0V83_004258</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBI9"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000048">
        <property type="entry name" value="DnaJ family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000001">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000024">
        <property type="entry name" value="Mitochondrial protein import protein MAS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="8">
        <location>
            <begin position="6"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="9">
        <location>
            <begin position="138"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="6">
        <location>
            <begin position="138"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="369"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4372484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="425" mass="46890" checksum="B9EBABD036EF72DE" modified="2023-11-08" version="1">MVKDTKFYEILGVSPDASDANLKTAYKKGALKWHPDKNAHNPEAADKFKDLSHAYEVLSDPQKRQLYDQYGEEGLEQGGMGGGGMAAEDLFAQFFGGGGGPFGGMFGGGMGGGREQGPKKARTISHVHKVSLEDIYRGKVSKLALQKSVICPKCHGVGGKEGAVKKCAGCDGRGMKHMMRQMGPMIQRFQTVCPDCQGEGEIIRDKDRCKQCNGKKTIIERKVLHVHVDRGVKSGHKIEFRGEGDQLPGVEPGDVVFEIEQKPHSRFQRKEDDLFYHAEIDLLTALAGGQIHIEHLDERWLTVDIIPGECISPGEVKVIREQGMPSYRHHDFGNLYIQFDVKFPERLGNEEGGPLSPEQIRALESVLPPRKVPDSLPPPDAMTEDFTLESVDPTRESARARGMTGMEDDDDDMHPGAERVQCASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBL0</accession>
    <name>A0A9W8YBL0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CMP/dCMP-type deaminase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004475</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBL0"/>
    <dbReference type="OrthoDB" id="252265at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008835">
        <property type="term" value="F:diaminohydroxyphosphoribosylaminopyrimidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF162">
        <property type="entry name" value="RIBOFLAVIN BIOSYNTHESIS PROTEIN PYRD, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18785">
        <property type="entry name" value="Inv-AAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="2">
        <location>
            <begin position="25"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="189" mass="20847" checksum="8C159C4C2D8AA0B1" modified="2023-11-08" version="1">MPEEWEGPVRLWDGPVPPAQAPDPNDHMQYMRLALDQAQESPPKPSNFRVGALLVNADTGAILSRGYTLECEGNTHAEQCCLLKFAQAHDLPEERVGEALPPNTVIYTTMEPCNLRLSGNLPCADRIIRAKGRDGEQRIKKVYLGVKEPEKFVGENQGRAKLEENGIECVHVPGLEERILSVATAGHTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC15</accession>
    <name>A0A9W8YC15_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_003285</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC15"/>
    <dbReference type="OrthoDB" id="5226580at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF10">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="335"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4372994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="419" mass="46890" checksum="C042EB79302CEE9D" modified="2023-11-08" version="1">MQDLGLSRNGRKTPTVGTTQEKSSAEKRAILGTYYLSAEFAHIWRKRTTMQYTRSLARNCQWFADHPEYSSDNLIAPLIRLSELKRRISDYFSYDEIEFSEINGEAMLDLSTGNFRSELQRLEESLPEGVRKNNTVRLSCDVLRVWIHECSLHSTLWNSLPTATSATITPSRVRMLQRAFASAQAYLQTLINAPSTSLHYYAVPTWAAWFYSTLVIVKIIFLHENSGSCATLGLPTLQSDVDDILPENNAGRSALQDVYQMTASLSVNATNNSSKGEREAWVIPLFQDFINKAILTAARYSCDDEPASKSLLRTMATLQQGLLAGLKKRIEKQNPRVQADSVASCTTASESDTNGPSNALSGERQVYEQAQAQTVHGGEPSGSSEFDIDSLPAWQEGQHPSMDDWMWDLIMDDVNMFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCH8</accession>
    <name>A0A9W8YCH8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Deacetylase sirtuin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_002816</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCH8"/>
    <dbReference type="OrthoDB" id="424302at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47651:SF17">
        <property type="entry name" value="DEACETYLASE SIRTUIN-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47651">
        <property type="entry name" value="NAD-DEPENDENT HISTONE DEACETYLASE HST4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="6">
        <location>
            <begin position="54"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="283"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4374077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="406" mass="43594" checksum="588A943679BDF369" modified="2023-11-08" version="1">MPRAPLLRVPYTDPLPAPKIIPTSASTASGAIAALVDFLSPSLLPSKTSTSQTTARSKLSTSPLYSSGDGGGGGGKTLLLTGAGISVASGLADYRGTNGTYTLNKTYRPIYFNEFCESHEARKRYWARSFLGWTNLEKARPNKAHEACGDLGQIGVVGSLITQNVDSFHPTAHPHLPTTELHGYLRSLVCLTCRSEYPRNTFQSQLSRLNPTWAAFLAEMLACGALDTENPDERRKKGLKTNPDGDVDIPDAPYTTFRYPACPVCLEKPPKRADGGGGTVRVQTDRDGAWDPKSEGGVLKPAVIMFGESIPAATKIAAEKAVDAADRILVVGSSLATYSAWRLVKMAKERHMPIGVLNMGGVRGEDAFFGDVEAANTGREAVRCSENAEKVLPQVVRILQEMKGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDR0</accession>
    <name>A0A9W8YDR0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2EXR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001944</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDR0"/>
    <dbReference type="OrthoDB" id="3792443at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045518">
        <property type="entry name" value="2EXR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20150">
        <property type="entry name" value="2EXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2EXR" evidence="1">
        <location>
            <begin position="71"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="296" mass="34330" checksum="BA44119AC53A13BD" modified="2023-11-08" version="1">MPAPRQYSRVLASTPFSLHHDLFPPLPPCDGHEQQDTATYLPAARAWATIAAKPHVPKSVPTSTLPTPNGFLRLPLELRFMIWEYALHGNYNEIHVDSRYDNWEKDPVKPSFLPQVCYVSQQTMEETVEVVLKGSKINMRRAHIDFFQAFLDVVPSRYELVRYLSFMDPRFYPEGAHKDIELAAKCKRLRVIELVFSHHDLLAPETRNGSGGYLNDAHHPKVQVLFAKYGFEQLSECDMLKKIIMEPRGYHNRNDQFALRLRVLGELLKQMFENKPTPQQVEVEYHWWPDTGVLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDT0</accession>
    <name>A0A9W8YDT0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nephrocystin 3-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_001974</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDT0"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="5">
        <location>
            <begin position="348"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="918"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="952"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="985"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1018"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="754"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="754"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="769"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="798"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4374896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1209" mass="135609" checksum="CA0E6150FE9D7952" modified="2023-11-08" version="1">MAAATKVQHSKFDPQQINPGLDVVYPNDDQALPKSSIKVDIVAIPGLGANPEWTWKHEKVDWLRDPNMLPRKIPNCRISVFQYQSQWFGRGSVNQRIENVANQLLYALDRSRGSETKTPIIFVCHCLGGIILEKALLTSRLRQNDFPSVYPWVAGCIFLGTPFHGTKTQSKAMVLAEMAETIGWGTPSDLVKILEKDSEILNSLLEDFALLARDAQIRLFCFFEQEKSDLVSLFIKSSLWKSQELIVDEPSATITSVEKLGLKSDHFHLNKYDGPKDGNFKYVSEEIRVTAQKAEGILKTRQNQRRQALVSDRTYHTMIDHLGKGFQDIDAAKNGTYKGIRGSKLSSVLELENYKIWQQQSSPKVLWVHGKAGTGQGTVAASAIESLQRSHDHGSIVTSFFCDQSDKQRRSLKGLLQMVVRQIIDLDQDLARHLLSDLTKSKDAGKQDFDPEETLKIPALWDALHKIAKDLPGGSVFIVIYGLEQLSKDSLDVFLQYMKETLHTSTLAVEDPEAAPVKWLILSRSGRPNIDKVLMSKALEINLEDAQNSAQVSDDLRTHISVSVDELSLPASLAYFVKRHVHSRAEDNWIYVSLVIQELKNAWVSGQTQHVEIRKLLESFPYGLTDMFEHVRKRVLDPRAEGFEYTKEILRCRICAYVAPTLRELAVMAGLPAEDRSDLDKLKAYIVRCGAFLTLRGDEWDEASNTVEWIDISAQEHLEEYAKDDLSLDLRDMQHGIIALRSLEYIYHAMEQEDDNQEGQGLDEDSDADSSKGEVVDHDDTKTTHPNEDDESEHNGEDDGTDAGEDDRDTTTSSASEAENHLDILSIDDLKYPIRYWMEHAKKAPRDVLEEFNFRHLFWQDEANARQEWWHSIEDVHGKSDQASVSSLHVAVILRFPELVEHLLENGWESDIHTTDSLGFQPLYYTCEGGQEEIVNALLGAGADINYVDNDKKPAALHAAASNGHPDIVINLLDRGANVDATSPDHGSALYAAAANADNDILKLLLDRGAKVNIIGGTSRRALNIAAFKGDLDAVRTLIERGADIDPNEDYWYGSALGAATRRGHDDVVRFLLSHGWNPNRPMKTYGSFLTAAATYNHFNVFETLLGEEGRELIIEQALQAAAQRGYAPIVKAILDKAVLNASFTLRPQKAFSLAAFYGRTEVLKLIPREEIEQDKLDEALYQATDNEHEETVKLLLKFNANPNAEGPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDY1</accession>
    <name>A0A9W8YDY1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein sof1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">sof1</name>
        <name evidence="10" type="ORF">N0V83_002050</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat DCAF13/WDSOF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDY1"/>
    <dbReference type="OrthoDB" id="10249065at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000657">
        <property type="entry name" value="U3 small nucleolar RNA associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000743">
        <property type="entry name" value="U3 small nucleolar RNA associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007287">
        <property type="entry name" value="Sof1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051733">
        <property type="entry name" value="WD_repeat_DCAF13/WDSOF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22851:SF0">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22851">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA U3 SNORNA ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04158">
        <property type="entry name" value="Sof1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="62"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="279"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="322"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Sof1-like protein" evidence="9">
        <location>
            <begin position="355"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="416"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="433"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4374971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="446" mass="50811" checksum="F31B42810EC9DA2C" modified="2023-11-08" version="1">MKIKALSRSSASAQAPGSTIAKVTRNLDPNLHPFERAREYTRALNATKIERMFAQPFIGDFEPGHVDGVYSFAKDPNSLEHFASGSGDGVVKVWDFTSRQERWQAQAHENLVKGMCWTRDKKLITCGSDRQIQLFEPYAQPSKSPPKATWHGNAAFTSVSHHRTLPSFAAASSVVSLYDTTRTSGAPVQNLVWPSAIDTINYVTFNQVETSILASCATDRAVILYDVRTSSPLHRTVLNFASNCISWNPMEAYNFAVASEDHNVYIFDMRNMKRALQVLKGHVAAVMSVEFSPTGEELVSGSYDRSVRLWERQKGHSRDVYHTKRMQRVFSVAWSPDNNYVLSGSDDGNVRLWRARASERQGVKSYALRQKLQYDDALKERYKHMPDIKRINRHRHIPKAVKKAGEIKTEELKAIKRKEENERRHTKKGQVRRKAEREKMVLAREQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEL4</accession>
    <name>A0A9W8YEL4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Imidazole glycerol phosphate synthase hisHF</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="9">Glutaminase</fullName>
                <ecNumber evidence="9">3.5.1.2</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="9">Cyclase</fullName>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="13" type="primary">HIS7</name>
        <name evidence="13" type="ORF">N0V83_002387</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The glutaminase domain produces the ammonia necessary for the cyclase domain to produce IGP and AICAR from PRFAR. The ammonia is channeled to the active site of the cyclase domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the C-terminal section; belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEL4"/>
    <dbReference type="OrthoDB" id="10254903at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01748">
        <property type="entry name" value="GATase1_IGP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04731">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000039">
        <property type="entry name" value="Imidazole glycerol phosphate synthase hisHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00278">
        <property type="entry name" value="HisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004651">
        <property type="entry name" value="HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050064">
        <property type="entry name" value="IGPS_HisA/HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014640">
        <property type="entry name" value="IGPS_HisHF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010139">
        <property type="entry name" value="Imidazole-glycPsynth_HisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01855">
        <property type="entry name" value="IMP_synth_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235:SF2">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE HISHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISF/H IGP SYNTHASE SUBUNIT HISF/H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036936">
        <property type="entry name" value="IGPS_HisHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 9 11" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="3 9 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="12">
        <location>
            <begin position="6"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="10">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="10">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="10">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR036936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR036936-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4375301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="548" mass="59429" checksum="68D2E455E17F945A" modified="2023-11-08" version="1">MPTVHLLDYVAGNVRSLVNAIEKVGYTVDWVKSPADVPSAEKLIIPGVGHFGHCLAQLEKAGYTEPIREHIKAGKPFMGICVGLQALFEGSEEDPQVKGLGIIPSRLRRFDDKNKSVPHIGWNSANTSPTDQTTDESLYGLRPTSKYYYVHSYAAPYEEGKLEKDGWAVATARYGNEEFVGAVAKDNIFATQFHPEKSGAAGLRVIKAFCNQKRSQKLPETPSVAETKEGLTRRVIACLDVRTNDAGDLVVTKGDQYDVREKDGAGSAVRNLGKPVDMAKKYYEQGADEVTFLNITSFRNCPVADTPMLEILRRTSETVFVPLTIGGGIRDTVDTDGTKISALDIATMYFKSGADKVSIGSDAVTAAEEYYARGSKLGGKTAIETISAAYGNQAVVISVDPKRTKTAGPKGEEYCWYQCTIKGGREGRDFDVRQLVTAVEAMGAGEILLNCIDKDGSNSGFDLELINDVKDAIKIPVIASSGAGHPGHFEEVFAQTRTDAALGGWNVSQGRVHGQAGQGLLARKRPNFEAFRGRHMTDCNCHIYLKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF39</accession>
    <name>A0A9W8YF39_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription factor TFIIIC triple barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001122</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF39"/>
    <dbReference type="OrthoDB" id="1877767at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019481">
        <property type="entry name" value="TFIIIC_triple_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10419">
        <property type="entry name" value="TFIIIC_sub6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor TFIIIC triple barrel" evidence="2">
        <location>
            <begin position="16"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="263" mass="28354" checksum="E793F60F7D977714" modified="2023-11-08" version="1">MDHADDDQWEYEYDETETEDFYITLDLSNVPNAQVPMVSQGRPGHPILLKSRLRALNAQRGQPTDISMDTSDGQETATMGELQIVGLHTTNPLVMHNGELLSCQWTSTIGSDMFFVKPDTSTSHLSEPLRSLPSVDLLAIGSAKLVARVGRLRPRDDLFDNSSEMQPATEPARLPSDAQNVGGPPMSAPGSEEQVVAEQVQPARMSFLTKLNQAKAKRGETSFLAVSNTSGGSRLVSKKGGEVSTPASQGTSQAHADTTMEGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF89</accession>
    <name>A0A9W8YF89_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_001257</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF89"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047499">
        <property type="term" value="F:calcium-independent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185:SF1">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="6">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="314"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="4">
        <location>
            <begin position="138"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="324"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4375977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="352" mass="39149" checksum="C491B53E64DFD474" modified="2023-11-08" version="1">MLGRLRYTVKDAIDHYLSLAETVFTSTSSDTDAAFDHRVLEKHIQKVIVEAPDHPDAHTDFVDPDLIDWSSPGSPRGCRTFVVASPTRGSGDNAALLRSYGTPLDSPSPGRIWEIGRATSAAPTFFSPIVIDMIKYGDGGLMANNPTRLAIQEANELWPGRSIGCLLSLGTGEDAPNQLIEQEHLPKEGWTDWIFTSLAPKPRFKLEVAKWCVHNTTRCNKVHYDVLANLDRDRLRSAYFRLNTPDIGKIGLEEWQRMPDMINLTNDYMAPRHMKEEKSTIAKQIIRLMTISQPRGSEPRNETQSELHTYANLLAPPPPIATIEYTQTQGGDEPTVDSSAGTYPPDTDTAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGC5</accession>
    <name>A0A9W8YGC5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Formamidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_002079</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGC5"/>
    <dbReference type="OrthoDB" id="9975579at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.580">
        <property type="entry name" value="Acetamidase/Formamidase-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004304">
        <property type="entry name" value="FmdA_AmdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054833">
        <property type="entry name" value="FormamaseFmdA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045496">
        <property type="entry name" value="FormamaseFmdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31891">
        <property type="entry name" value="FORMAMIDASE C869.04-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31891:SF1">
        <property type="entry name" value="FORMAMIDASE C869.04-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03069">
        <property type="entry name" value="FmdA_AmdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141130">
        <property type="entry name" value="Acetamidase/Formamidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4375000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="412" mass="45093" checksum="7AD8CEDF42785FBE" modified="2023-11-08" version="1">MPKEGLRYAAKVSLDKPAAEQECLHNRWHPDNPVCGTIKNNEVVKIECVDWTGGQIGNNDSADDVRDVDLTKVHYLTGPFEIETAEPGDVLVVEIQDVQPMQEQPWGFTGVFAKENGGGFLDEIYPKPAKAIWDFEGIFCSSRHIPGVRFAGLIHPGILGCAPSPEVLAEWNRREGELISTHSHLGRVVAEPPNPKSVHAGSADGDLKEKIGKEGARTIPGRPEHGGNCDIKNLSRGSKVFLPVHVKGAKFSVGDLHFSQGDGEISFCGAIEMAGTITIKFSVMKNGVEQLGMKSPIYVPGPVEPQFGPGRYIYFEGFSVDEHGKQHYMDTTVAYRQTSLRVIEYLRRYGYNDYQIYLLLSCAPVQGHVAGIVDIPNACTTMGLPMDIFDFDISPHVPAKKQDLGTCAFASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGG5</accession>
    <name>A0A9W8YGG5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Gas1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGG5"/>
    <dbReference type="OrthoDB" id="3241054at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021476">
        <property type="entry name" value="Egh16-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34618:SF3">
        <property type="entry name" value="GEGH 16 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34618">
        <property type="entry name" value="SURFACE PROTEIN MAS1, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11327">
        <property type="entry name" value="Egh16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040904399" description="Gas1-like protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="28534" checksum="752177FE411E986C" modified="2023-11-08" version="1">MYFHATSLALAALITTVHSHAAILAAVGDKGQSQGFLVDPEIARNCTTISPCQQDATIIRDSEISQNIVNACGRTELNGNIDIGEQTENELAAGRMATVTKGSMLAVTIHQVNADGAGPFECDMDETSNAVTTFTPLKVSNNVPGTFGLSQAKEQDFVINVQMPDNFNCIGASTGNICTVRCRNNAVAGPFGGCFAVQQTDGTGRTNASAAAVGTSQTLEGISAQILQNQKDLPAAIAANQAAGAAGGNEGASAISGACLIQYHLIEASIDMKLTSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH50</accession>
    <name>A0A9W8YH50_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">O-methyltransferase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_000739</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH50"/>
    <dbReference type="OrthoDB" id="2410195at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF1">
        <property type="entry name" value="HYPOTHETICAL O-METHYLTRANSFERASE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005739">
        <property type="entry name" value="O-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="5">
        <location>
            <begin position="209"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR005739-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4377909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="381" mass="42741" checksum="9E2CFC73AA23BBFE" modified="2023-11-08" version="1">MEAAMEQVRTLYEKANAGERQRIQEQLRDLQRDLYTDWEVVFSLAVGPLHWAFIRVGLDLSIFKTLASSTTPVTHEDFVKKTGAAPTLMAHLLRDMASFGLIEEAGKDVFRANRITRLFTDANVIGAEPHVSAFHLPVASVLPEYLKDHKYQDMTNPKDLPVKYALKTDLTPFEYLKQHPEQMKALGHIMVLDAVDSWVSSYPVEKEIGSFKASDDSALLVDIGGGFGQHSVAFQKKFPQLPGRIVVQDIPSTLAHAPSADGIEFQRHDFFITQPIKGAKFYYLRHIMHDWADEDCIRILTNIIPALGPDSLILIDEVVLPETKVPWQVGMMDISMMACLGGIERTNGDWESLLDRAGLKVAHVHVYDDVRFHGIIAAVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIJ3</accession>
    <name>A0A9W8YIJ3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000826</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIJ3"/>
    <dbReference type="OrthoDB" id="5015991at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="113" mass="13484" checksum="BBB7699823CEB708" modified="2023-11-08" version="1">MCFFDQHRFACGDWKWGHFRQHCAKEYRIGETCGMKLIMQTVATGTKCKLCEKIDTKMRRRQAEVERIGRWQREPRKFAASIEKSVDMIRGLDGEIYELSCERNRRLQQIGQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIX5</accession>
    <name>A0A9W8YIX5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I, II, and III subunit RPABC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">RPB10</name>
        <name evidence="8" type="ORF">N0V83_000195</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the archaeal Rpo10/eukaryotic RPB10 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIX5"/>
    <dbReference type="OrthoDB" id="10258858at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000024">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00250">
        <property type="entry name" value="RNApol_arch_Rpo10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023580">
        <property type="entry name" value="RNA_pol_su_RPB10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020789">
        <property type="entry name" value="RNA_pol_suN_Zn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000268">
        <property type="entry name" value="RPABC5/Rpb10"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003089">
        <property type="entry name" value="PRK04016.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431:SF3">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01194">
        <property type="entry name" value="RNA_pol_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005653">
        <property type="entry name" value="RNA_pol_N/8_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46924">
        <property type="entry name" value="RNA polymerase subunit RPB10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01112">
        <property type="entry name" value="RNA_POL_N_8KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="82" mass="9444" checksum="4F738A61F5FCF5AD" modified="2023-11-08" version="1">MIVPIRCFSCGKVTGDLWEKYMALLSEGTEEVDALDAIGLSRYCCRRMILTHVDLIEKLLKYVPSGDRALQQKKARKRRSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJ14</accession>
    <name>A0A9W8YJ14_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fungal specific transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">DAL81_1</name>
        <name evidence="5" type="ORF">N0V83_000240</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJ14"/>
    <dbReference type="OrthoDB" id="2264294at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001080">
        <property type="term" value="P:nitrogen catabolite activation of transcription from RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050797">
        <property type="entry name" value="Carb_Metab_Trans_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN DAL81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="32"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="383"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4377415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="722" mass="81725" checksum="8DC8C4A8B629B21B" modified="2023-11-08" version="1">MNFSPTQPFSPNEFFPAPAELPNGAKIRARRPCDPCRRRKSRCEVVGGSPPCLLCRFHRQDCTFNDDPQPRKRRAVTLDGDSAPAKQFQAPHAAETIKSTGLSPRDGLKIRQDLPIDDYANLRGPSLLKKTLGLQSHRHSRFIGSTSEYEQLLLELDHSGGNQETVLGLMALRKVASDDTTFALSPDAGTQNHQNDIPDLDAIETIVAPHGQALIHLYFRIVHPSFPVLHKKVFLEKYERTHREFSPPLLAAVYLLALNWWSYSTELALLPEPDVSHLEMLALKSLSNIIHRPKLSTIQAGLLLLQRPEGDSWALTSQLVALGQDLGLHLDCTTWRIPSWERGLRKRLAWAIFMQDKWGALVHGRPSHITVTDWDAQPLQNDDFPENAADEDDEDGSTEVEKGRTLFCEMIKLTQILALILSSFYTLKAEREFAHRAPEGVRWILETAKPLQLALRQWYSSLPIALKMEDLGARKLSSTGYLHLGYYAVEITLHRRIIRSLSSVDDPTLRSICRQAAETRLRSAMDFVQHLRPEHLQSFWYSASKYNFALVGTFISLLWVTSTDREEAQTYRSRFDEYRWMLRLSSKSADFLERTLTMLATSTGELVKGIPEKPDPEWVLNRYQRRARYGRSASSSAVSVVGLNRSDESAEVVPPGVAYQSESELHEDTSPENVVGRTPSESEMVVGNEWNVDYAWFNNALGEVDGFADDSQLGLDFQQQQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH36</accession>
    <name>A0A9W9CH36_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">rRNA-processing protein EFG1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">rRNA-processing protein efg1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">EFG1</name>
        <name evidence="11" type="ORF">N0V83_010875</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in rRNA processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EFG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH36"/>
    <dbReference type="OrthoDB" id="47732at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019310">
        <property type="entry name" value="Efg1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050786">
        <property type="entry name" value="EFG1_rRNA-proc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33911">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EFG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33911:SF1">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EFG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10153">
        <property type="entry name" value="Efg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="176"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="233"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="88"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="39"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="194"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4361934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="290" mass="33304" checksum="95399FFF42D98285" modified="2023-11-08" version="1">MSQKRKHAEFADIEPSNPKPFKKHRSFNPGDSHQKSKRPQVSNDISKTTSTNALKSRIRDLKRLLAHVENVEGHKMSAGMRIERERELEACEHELREQVESKREAEYRQKMIGKYHQVRFFDRQKATRILKKLKKELSTSEDQSQKTKLNQRIHNAEVDVNYAIYYPLMKPYPSLYPKSKKGKSAEADDEDDGANDKGNREVDGPKGDAEMWRAIEKAMAEGTLNALRYSKDGMPAAPATKELKKFVKKPAMKREEAPEGMNRRERRKAGLAKAQAAQDEDQDSDGGFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHT4</accession>
    <name>A0A9W9CHT4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHT4"/>
    <dbReference type="OrthoDB" id="3795483at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="407" mass="46326" checksum="7B4A04C76FD1C437" modified="2023-11-08" version="1">MADDKAPSLFMNLPLEVRHGIFEHATTRDGKPKKLLRYWFERKEAKEIIAKTAATNPNGQMPQIVYDDDDDEDSDMDEEEVEDQDSAGEDEDADEDEDQEDEDDMDQDDAEDAEADMLEEDGVENMDEDQDDEGVDDPEQVEADMEDEHDTELGEADDAETDTQGDNDADEDNDEAMAEDDNNIQGEETGHGGDTADADATQTPHPPRPIVRAHHKWRHIPNFMRLTHCPPPVELLLTSKQLNDEAKNWYYDVAVLRIEATGSFSHTSFYEEAFSQITEAAFSPMENIRKVEVTFVWDTTWIRADTTGCVDAIFPALLRQRANFVYKILQQAPDLQEVVIHWHDSARDHESANLRIDVLEPFDNLPATVEIIEHYIPADSKPNSRSIAGKRRVEFQNILDMGLDRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHW0</accession>
    <name>A0A9W9CHW0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010339</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHW0"/>
    <dbReference type="OrthoDB" id="3794928at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="102"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="658"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="596"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="658"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="692" mass="77408" checksum="6D71C590B98E99F3" modified="2023-11-08" version="1">MVDEDEDDDPSFVGTESEHSSDDEEREALSDEDREELSDDGDRLEDDKGEELSDEDVEDSSDDDGEEFDEEISDAEVNCLLREAKDLSVPIQIPHIATAVRPGTVAAERHDKDGKATTKANATEEGELPGKRDDPPAALAPTASSKPPPSAFGNASFVATTSASLLTPGHGPTLNSTTARNNNLELTLPESPLMNLSNKLLINEVFKIQEIDRKCHPCRGLDAQGAQCKSSVPRCHSLSIKLVEDSIGIAEVMKLEEVQDLATYNMCDAHTKQSAKLAREWIRLAKLTKAARKYWLSQQSSLSVEFKASKGKYAEFAAAEVQRILAQDETAVRAEKEKAELCNEPQKTTTKLQTKIDNIESEVSSTEEPNETSKKILAEYLDEISALKMQIYNITTENSSIKKARESTENALAEDEKRHQLDLLDAKDLYKVSQKTVETLRREISALTSKVGELTTENSSLREGQEATKDALVKERKRDQQNLQDAKGLYEGSQRTVVALRKEVDGLNSCIGELKTIKSSLNISLESTQDALAKEQVHSRELEIRLEERVALGIKTQDSPETKLEKYLREELNKSQRHVLQRQKDFDKLRDDHVRLQITNATLESQVKQIEALKEELSRASYRESQELAERNSDLLNQLHHTRAQFERLKAGLAAMEKANEGEASKSKFSLRDIYKRTKHDPSRLLTPPTDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI24</accession>
    <name>A0A9W9CI24_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009862</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI24"/>
    <dbReference type="OrthoDB" id="4338954at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="148" mass="16695" checksum="7A70056490DF9C64" modified="2023-11-08" version="1">MLFTRISPLLRQRLFTTSARARHVISMEDAAAAQLTATASAVPRLGRVARWYLPTMAAIALGFGITNALNEANRKVHMALEPTQEEKNMMLMDSYGERSSLEDMERAIAGLEARVMSQKNRNKILEEAYGDRSSIKDMERAMELYEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIG5</accession>
    <name>A0A9W9CIG5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PLP-dependent transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_009418</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIG5"/>
    <dbReference type="OrthoDB" id="3512640at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000072">
        <property type="entry name" value="Putative cystathionine beta-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000066">
        <property type="entry name" value="Putative cystathionine beta-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF35">
        <property type="entry name" value="CYSTATHIONINE GAMMA-SYNTHASE (AFU_ORTHOLOGUE AFUA_7G01590)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4363965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="445" mass="48317" checksum="4AC01548F39E10A6" modified="2023-11-08" version="1">MSSSFHPATYGIHADDRINIVTDVAPPIHTSTTFRYPHNSDELQPVPTEGEFATLQTPLVYSRLASANINRLETILAPLIYPNSEEEEFEQLANHVISYSSGLSAFHALLVNVVPKVIAISAGYHGCHGVIDLHKKMHGVKTVDLHADDAAWDQAGLGKGDLVHLETPLNPTGEAYEIKKYADRAHARGAFLSVDATFGPPGLQDPFAWGADMVMHSGTKYIGGHSDMLCGIIATAKGADGLKRAKTMRMERIFLGSVLGSLEGWLGVRSLRTMELRVQRQASNAEKLVSWLNDSSNGQGDENEVIKKVVSKVVHASLQKEDMGWLKKQMPNGFGPVFSIYTHTSSQARQLPSNLHLFHHATSLGGVESLIEWRRMSDSEVDETLLRVSVGVENWEDLKNDLLLAFKALADAGGSAEKKVNGTKVGDVQLGQEGAKMPEVGAGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIK5</accession>
    <name>A0A9W9CIK5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_009584</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIK5"/>
    <dbReference type="OrthoDB" id="3647at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861:SF1">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="4">
        <location>
            <begin position="61"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4364129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="287" mass="31732" checksum="D06DFD01907E8A30" modified="2023-11-08" version="1">MADQESGNGGVTAGGSSTSTQNTQYDKIGSKYNKIKTLPATEPEVPSVLKALGGIKGKRCLDLACGTGKYTSLLLSHGASSVHAYDISPAMISAAQTTYPPSTYPNLHFSVADCSIPGTLPHSSPSSSSSDEKFDIVFAGWFLNYAGTRRELTNMFRVIEESLAPPRQGEEGGRFIGVTTNVHDALMHLEKLNFYGLDVLVLDPHYKEEEEGEVLGIKARVVVQGETPFQFDVFQFRREVYERCAEEAGLRLRWREVVVPEDERREGGYWERFLERPTFVVVEAVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIV3</accession>
    <name>A0A9W9CIV3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009322</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIV3"/>
    <dbReference type="OrthoDB" id="408743at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050411">
        <property type="entry name" value="AlphaKG_dependent_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696">
        <property type="entry name" value="GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696:SF21">
        <property type="entry name" value="TAUD_TFDA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="2">
        <location>
            <begin position="43"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="253" mass="28231" checksum="1EC516D7A3E675F0" modified="2023-11-08" version="1">MASGQQRTGTFGYEHDRAFPLALQARSDWKRTVDETVAHLESLSKSGALFDLVRQHGGALLIRGLPITNAETYSRIAHAFGFAAHDEVGRPPIRTVLAKNVKTANEGPPELPIWPHNEYGWSTHSPAWLTFSCLAVPESGGATPIISSIGLAHKLEKEAPEFYRLLLQKGVRYLYRYGREQVESTTGASVFAAYGQHVQEGDDEATIKEKIETEVKRHSELFEWHQDGSLSVTHIVPSKELTLLYDHLTHHVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ85</accession>
    <name>A0A9W9CJ85_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_008204</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ85"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002974">
        <property type="entry name" value="Cyt_P450_E_CYP52_ascomycetes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047146">
        <property type="entry name" value="Cyt_P450_E_CYP52_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002402">
        <property type="entry name" value="Cyt_P450_E_grp-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287:SF1">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00464">
        <property type="entry name" value="EP450II"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01239">
        <property type="entry name" value="EP450IICYP52"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4365584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="448" mass="50733" checksum="61F289B54875B211" modified="2023-11-08" version="1">MGLLLSLLPAVAAFFIIYCVIRHVRQSAKAKSLGCRPTPLFRPWDVLGVQNFKIELNGMKTNRLSYAFSDRKNEMSAKMGRDCKTFRIYYPPGETWFYTFDPKNLQAVLATQFQDFQQPAYRVGAFEALLGLGIFSANGAKWEHSRALLRPQFMREQIADLGLFEKHVKDLLDALPAAEEPGDWTPTFNMQPLLERFTMDIATEFLFGESVHSQKDDSTDTSTLFSKKEMKQFVEAFFAAEKTTAKSLLYGDLFWLTHNRKFKEQCKAVHSFVDTYVQRHLRDGSSEKKPETEKYVVLDAIVADVEDPHELRSQLLNILLAGSDTISGTLGFLLASLAQYPHIFKKLRKVILDDFGSFDRPKQITLSGLKNCSYLQWCLNEVLRVYPAVPVNFREAVRDTTLPVGGGSDGASPVFIPKGHLVAWEVRLYVVSQLLCLWLLLSANISSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJE4</accession>
    <name>A0A9W9CJE4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription factor TFIIIC subunit tfc4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TFC4</name>
        <name evidence="2" type="ORF">N0V83_008400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="9991317at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039340">
        <property type="entry name" value="Tfc4/TFIIIC-102/Sfc4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23082:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23082">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIIC TFIIIC , POLYPEPTIDE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="753"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="260"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1293" mass="148597" checksum="392B13F3254F0BC1" modified="2023-11-08" version="1">MNPNNGYYNQWPARQPPNNRRFLPVAGGFADAPPNLTRPQAQSHRAQHPVQFPHGQQSFLSQPVYQQPRHQPQPHHPPHIPLAPLIPLSAVEDLQPPVPFTNGAFQDPFNRAYATNGPEVYEGFIPAPGREDEDSYPNLPTAFRDSLYQDRGFESSEEEDEYERMRAEFEEEEELRQIEEKDDSEVDRDYSEEDAQEDEGDPDEMELDEDFEDEEERIRSKKGKPAARVTTSLRDTRSARGVRGGRRGRPSGRSRGGFRGSASTRGNKAGKQGRPRGPRGPRAVADPGPEFKDLQRHANERFIAQDYDAALEYAQKAIQVNPEIFDAYNITSEIYAAMGQEDNSIAALIAGAPTKRDAGLWQFLIERINKLDSEKYSEYTEEVKTGAILGCLNWIIQLDHTSANYEARSHKLEIEARLGHASKCITLGLKMLKTRRERKEDPDTAVLKIMAMMGTSSAKQTKIHLDKLITSFDEAIAVFTRPRRDPFTSDLDWELINIYLDLLDRSKKYDHAISRLKVLSRWKQGRRKETYWNEQNDDREFDIQDEPRRVAVPQYVRGLQNAKYGETLPLEIRMKLGLFRLRKSPDDFQEAMHHLELLEPDDHGPNALVWDYEDLFRIIADALHATGHDKDALRFYGPLYSANSSELSLMSYIGLHTCYKNQEEDEKAKQIIPILKSWPAENYDDLAVLAKFFEDQGMWQDAAQRAERIYRDKYGHKLKRVGFRAYDELREYHYHQRKQARGRYAVRKSVVKKNRKMLKKATAPGRDDEDSTNESANKELPSLGESTDRPTQGLFRTRRAKPPKTQIFLPFQTDVEPVAECEPKRTTIEGTDVPLQAIDHRFFRRRLDNLASDYADDLKAARDQHREIVSSFQRLDALSEPAEDGDYDATNELFSITRELIEEFSTFDLFYANKKEDLTTYFRRIGNGDIWKESALMVLAVVANNVEDGETDPELKEKPDGPPQEFWGIHFDKWCDAFGQYAILLARDGDDQQCFATLDIVSQSNIFHRSRKYIQQLQLCRLTCALAVDHSNQASIATRWLMKQYPFGTDLFRLYSAVNRLCSFPEGFATGPAHKVLFRYIKTMDYALLSPEQRVWYNFRDPKDSKRGYKNDMNVEGVDKVKDHDPALFALYGHVLMCGGSYMGALNYYFRAFAMIPEDPVVSLSIGVAYIQHSMKRLSENRQFQIQQGLAFTYRYYELRTKEDVAIHCQEAEFNVGRMWHGLGLVTLALPAYERCIELGTRVKQEANDRCSEGNWAHEDFATEAAFAIQSIYAVSGNFEGARKVTEEVLVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJG6</accession>
    <name>A0A9W9CJG6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008177</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJG6"/>
    <dbReference type="OrthoDB" id="10042665at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19481">
        <property type="entry name" value="RecA-like_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054289">
        <property type="entry name" value="DUF7025"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46411:SF3">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46411">
        <property type="entry name" value="FAMILY ATPASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22942">
        <property type="entry name" value="DUF7025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="487"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="693" mass="79412" checksum="36CC449FCDE2AB4E" modified="2023-11-08" version="1">MRKGFIITRGHTSRPSVRFISKAKEKSTAIPSRNGKTRLNRATQHRTCPPEDYENTSSSSIDEKSEAKDLDGIEVDKSNPLIEKDIFAYVNETTVDDGQRGELCELHSYDARVNSKGEEVLLRTGSKYEFNDEKEKSPEAALVFTRHVRFGKVRYTSLEIKSPYIKKALKEVIGSYPGVDIDLDRSIRLLDKPQCLFHYRHELEQYAAAADDRPMKEHVIFALKYMEKALCKEISTYEDMAQVEGAIPKLRYEDLWMAFKPGALVYTKKEGVERIYKFKEMYETEDDNDRPLWKLNTEVILYNGKDFGFLDRDVDIYRFEGYKVLTDLKVFPLVFHKDYKRVYEELLLRGRKYVSLRGVQHRITWYEKGNNQTRHRIMLDPYEHNMNRGPHRLDFSYARVIETGSGDTPELSEEHLLLCDDRIPGFSLTTKVWGFFEVNKIEDVEFKDTAFEKLVLAQEKKDMIEALVRAGQGSATYFDDMIKGKGKGIIFLLHGPPGVGKTFTAESIAEFIQRPLYTVSCNDLGGPGPLAEIKLSAALSQATKWNAIVLIDEADVFMAERSLNDLARNELVSVLLRVLEYFEGILFLTTNRAKTIDPAFKSRIHFTIAYPDLSTEAKCDLWNLFITLGPAGQAPDWVDKDFLQQAAAYDMNGRQIKNAVRVAYALAHDKKRELLPGDIFAVLRSSESFEANE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJQ3</accession>
    <name>A0A9W9CJQ3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_008419</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJQ3"/>
    <dbReference type="OrthoDB" id="2588832at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF28">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10880)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="9">
        <location>
            <begin position="3"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4365798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="511" mass="57073" checksum="7CFD7871FB615205" modified="2023-11-08" version="1">MPHATLTRESVAKHNSPEDLWCIIDHKVYDLTDFVDAHPGGSVVLEQVAGTDATAAFYNLHRQEVLEKYTDLCIGTIEGEKSEVIVPKPGDLSPVPYAEPLWLRPQFKSPYFKESHRALQKEIRKFVDIHVTPEAQQKEKDGTYISQELIDKMAANDMLAMRLGPGKHLHGKNLLGVVKGEEFDYLHDLVQAQEGVRANARGFQDGNMAGMTISLTAVLQWMPESELKTRVVNEVLTGKKKMCLAITEAFAGSDVAGLRTTATLTPDGKHYIIRGTKKWITNGMFCDYFVTGCKTEKGFSVILIERDDTVETKLIKTAYSTTAGTAFVEFANTKVPVDHLLGQEHQGFIVIMSNFNHERFMMACAVIRQSRTVVEECLKWCSQRIVFGKKLIEQPAIRQKLAKMISHIEANQAWLESIAYQMCHMSYAQQSKLLGGPIGLLKSYATRSAHEIADEAVNIFGGRGLTQTGMGRVIEQFHRTYKFDAILGGTEEILGDLGVRQAMRGFPKSML</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJZ6</accession>
    <name>A0A9W9CJZ6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_007848</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJZ6"/>
    <dbReference type="OrthoDB" id="3770506at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4366212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="443" mass="50699" checksum="BA081CB9F0FC2E64" modified="2023-11-08" version="1">MDRLSALPPELTSHICRYLGRNVNQDDTSEVEYDDSDLCSLRMSCRTLYINTLYDASIRFPLGHIMLTFNYSSLVILLQLTANESLNQRIRSIRLSQDDGLRGGAVWPRDVWRSQLSYTGTGEANSFASSAAAVDMLTRCLRNLRPSVNFEVITYVPGVEDVFGQTAVLEAVRAAQLPRKVVRVPIDLMSITEPDGGNLTDIFGLYRPLILGASILIPAFHNEEIDKHDPDRHYVHHFRLAHPNLDQVFRAIRVIPTISVKGCNTDPELDLDDDCHDFFVSLIARNTFSQLSRIELTDLYVSGRRLSRFIKENASSLRVFSVEHVKLMDGSWDNIVDTLRKFCQLYHLSMCGGLYQKKHTFPTEAWKALPPDKADTKLTVYVWGQWNVSYYLCHWQKHFKVYRNKPDKVSSYVKICLFNIPLVSRGDSENEVVLRNYAFEYDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK05</accession>
    <name>A0A9W9CK05_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_007878</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nuclease type I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK05"/>
    <dbReference type="OrthoDB" id="441446at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11010">
        <property type="entry name" value="S1-P1_nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.575.10">
        <property type="entry name" value="P1 Nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008947">
        <property type="entry name" value="PLipase_C/P1_nuclease_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003154">
        <property type="entry name" value="S1/P1nuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33146">
        <property type="entry name" value="ENDONUCLEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33146:SF26">
        <property type="entry name" value="ENDONUCLEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02265">
        <property type="entry name" value="S1-P1_nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48537">
        <property type="entry name" value="Phospholipase C/P1 nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4366242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="279" mass="31062" checksum="12452B818D07C2E7" modified="2023-11-08" version="1">MAETFFMPETSSVLAEILEPQYNGSIGRAAAWADAYAHTSEGRFSYQWHWIDTHDHAPEYCNLDYKNDCAKGGCVVSAIANQTGILRECIDRVKSSSWSGGTNLTCSYALKWVSHFLGDIHQPLHANARAIGGNTYRVTFGGASTQLHAVWDAYIPYFAANVSHPFSNQSIDPFFTGLVSRIRKDGFYEAPYMWLACSDPSTPEVCAESWAKESNKWDCDYVWSRVRNDTDLGTNGYATGAVPIVELQISKAALRLGTWLNNLVVGEKNAHEEQEIIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKT1</accession>
    <name>A0A9W9CKT1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cytochrome c oxidase assembly protein cox11, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">COX11</name>
        <name evidence="7" type="ORF">N0V83_005976</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Exerts its effect at some terminal stage of cytochrome c oxidase synthesis, probably by being involved in the insertion of the copper B into subunit I.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKT1"/>
    <dbReference type="OrthoDB" id="1704689at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.370.10:FF:000001">
        <property type="entry name" value="COX11 cytochrome c oxidase assembly homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.370.10">
        <property type="entry name" value="Ctag/Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00155">
        <property type="entry name" value="CtaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023471">
        <property type="entry name" value="CtaG/Cox11_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007533">
        <property type="entry name" value="Cyt_c_oxidase_assmbl_CtaG"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003465">
        <property type="entry name" value="PRK05089.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21320:SF3">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX11, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21320">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04442">
        <property type="entry name" value="CtaG_Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005413">
        <property type="entry name" value="COX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110111">
        <property type="entry name" value="Ctag/Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4368894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="202" mass="22767" checksum="837064BFFEDA4F89" modified="2023-11-08" version="1">MHAQYKIKNRTVMNYVFSVIVGFVAITYGSVPMYKMICQQTGWGGQPIKSAAHGGDSDRDPSERLKPVENHPRIRITFNGSVSDVLPWKFVPQQREVRVLPGETALAFYTATNKSDEDIIGVATYSVTPGQVAPYFSKIQCFCFEEQRLNAGETVDMPVFFYIDPEFVTDVNMKGIETVTLSYTFFKAKYDKDGHLRPVPMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKX2</accession>
    <name>A0A9W9CKX2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_006605</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKX2"/>
    <dbReference type="OrthoDB" id="5086884at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17325">
        <property type="entry name" value="MFS_MdtG_SLC18_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050930">
        <property type="entry name" value="MFS_Vesicular_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506:SF37">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="308"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="345"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="425"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="31"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="490" mass="52144" checksum="3DE16D15ED985727" modified="2023-11-08" version="1">MKPVSTIFTTMGLSSSSPPRGLKWRSHTVFIVATVGMGAFTDMFLYGLIVPVLPFMLKDRIDMPDSRIQGTISNLLAAYAAASCAASPIAGVFADKFSLSRQIPFVLGLVLLLLATVLFALGQSVVVLAIARVLQGASGGVVWTLGLAIIIETVSQENLGKTMGTVFSFVSVAALFSPIVGGLLYAKTGYKGVFGVGIGLVAVDFVLRLLMVEKNVAAKHSTSPARLLEQESDGQMEDSEQTPLLPTTASAISRYRLPEPKNRLTRAFPILLVFSDPGLLTAIWIGFMQAVLLGAFDATVPLVASEQFGFNSLAAGLLFLPLGGADFFLGPIFGWGVDRYGTKPFAVLGFTWLVPMLVLLRLPTDPSIGTKLDLGHLIALYASVLALNGVGLVSDSMPAAKAGNIVEKYYKANEYMFEQPPYAQLYGINSMIWSGGLSIGPLVAGALRERIGYGNMNAVLAGICVLTAILAAHFIGRKEEDTSSRIEHEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL15</accession>
    <name>A0A9W9CL15_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006771</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL15"/>
    <dbReference type="OrthoDB" id="429143at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF284">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="1">
        <location>
            <begin position="44"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="494" mass="54674" checksum="1FA240AAF0454F8B" modified="2023-11-08" version="1">MEERASIPVGPPVTKPLPSYWHSPKSPLANVIEPETDKLTDPYDFAIIGSGISGTMIAHYLLKSRPNARIVMLEAREICSGATGRNGGHTKAASYRSYMQHVQELGKEEALKIARLEYANITETHSIAHELGIDCESKLCDTVDVIYDQATFALGKSAIQALRADASEEEKDEGKMACYQVYEKEEDIEQNFSVASNNINPEVKEKEDLAGVFRYSAGRIHAYRFATGVLKECVKNGLQLCTNTPVHDIRASTRSIDNTGPMWDVFTQYTTLSQLVTLCLHPMGIPPYLLKDLQGAIVPLRGQITAQRPGKATNFTTPLPTTYSFIYRNGYEYMIPRPLSDGVQHIIIGGGLGRLPQAGASEYGTVDDSTLNRQVSAYLRESLMGYFDNENWGEKDEEEASGRVVQEWSGIMGATADGRPFVGAVPDKKGLWISAGFNGHGMVLCLKSAEALVKMIERDSILGKPDWFPESFLITEERLKECKFRGRTDMQVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL62</accession>
    <name>A0A9W9CL62_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytokinesis regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005341</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL62"/>
    <dbReference type="OrthoDB" id="19159at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990334">
        <property type="term" value="C:Bfa1-Bub2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034586">
        <property type="entry name" value="Bfa1/Byr4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35140">
        <property type="entry name" value="MITOTIC CHECK POINT PROTEIN BFA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35140:SF1">
        <property type="entry name" value="MITOTIC CHECK POINT PROTEIN BFA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="321"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="762"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="334"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="443"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="555"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="874" mass="94261" checksum="9FA95B04B24BFBE1" modified="2023-11-08" version="1">MAAGPYRGSFAARMASITSAASNTTANMNNMESWDDEFDADELLFKSSNAQSLSSRMSVRSESNAGGEDDWHVLITPHDDPSSARAITSAAKQAGIPIPNVAPSALLGGTIKRLGKKPSSRKLDVDADFDIDFELPSNPGEGLKLKAPTPRTPADDFDDCGTDWGEGSLGIRFAGTRNARGARSSSVSAMSPSLGSCMTIESEDDDLLGLVLPNEPLDFNARLDKLKKSEQLPTPDASPLPPQHSRDLPPTSAPGPSLVDDFSSPPPAAVTMPSPPLESQVAPSPKPKPADDEEDFLDGLDFGGDEIFDSQKLTLNRNVVVKKPAAKPTPPSAARPASTLTFSDRPTTSRIPRPLPSSSRTRLTPVYETGASTQGQSKPIPTTTSAQLLRAKRSAPVLRNSNLNQPSRMPFLPAGNSTAQSHHVMARTSQPHLRRDSDPRRPQSPSMRPFSRLSGHQNETPSRAGVRRDLAPSALARHPPPKKLTQPQRKRNFGDGHELDMFDDLPTSATKEKAFEKVPRNVASNKTLRQQPSVSRLPMPDRMATPLPQTPRSPPKPDHTPRFARDTAASRNAREQRLAGTRSRADGPIAPRPISWAAQVMARSPHTSPSAHRKKGSGQKPQLIRQMTQPFTHNEKGMVYNPTMQRWEGNEEALVSFSHPNTSTTTLALTTASTPTFAPPGNPLSRPHERSHSISHTALSNIHAAQKSFSSRAAKLAAAPAPVPAPSPPRPALISQMAVPRGVQYEGRMVFDPVKMTWLKAARPSNDPRSPSELDDDDEDPFAGLDDLKDNESVAGSNAAGGAGTPNPDEPSFVGEEFDVGPGFIRRQRDEEAIWRRRVEGWVGGIRDTGEQRGGWRWAIRDFAASASAGASAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLE1</accession>
    <name>A0A9W9CLE1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Transcription initiation factor IIA subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">toa2</name>
        <name evidence="12" type="ORF">N0V83_006594</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">TFIIA is a heterodimer composed of the large TOA1 and the small TOA2 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TFIIA subunit 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLE1"/>
    <dbReference type="OrthoDB" id="586585at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10014">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10145">
        <property type="entry name" value="TFIIA_gamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.190:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIA subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.18.10:FF:000003">
        <property type="entry name" value="Transcription initiation factor IIA subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.190">
        <property type="entry name" value="Transcription factor IIA gamma subunit, alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009083">
        <property type="entry name" value="TFIIA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003194">
        <property type="entry name" value="TFIIA_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015871">
        <property type="entry name" value="TFIIA_gsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015872">
        <property type="entry name" value="TFIIA_gsu_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10966">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02751">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02268">
        <property type="entry name" value="TFIIA_gamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009415">
        <property type="entry name" value="Hum_TFIIA_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47396">
        <property type="entry name" value="Transcription factor IIA (TFIIA), alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit N-terminal" evidence="10">
        <location>
            <begin position="7"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit C-terminal" evidence="11">
        <location>
            <begin position="64"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR009415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4368238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="112" mass="12899" checksum="7EC2610CA79A81C8" modified="2023-11-08" version="1">MADNTKYYELYRRSSLGGALTDTLDNLITERRIEPQLAMKILINFDKAVADVLSDKVKSRLTFKGHLDTYRFCDDVWTFIIKDINFKLDNTNQVHADRVKIVSCNAKRPGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLP2</accession>
    <name>A0A9W9CLP2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLP2"/>
    <dbReference type="OrthoDB" id="1393670at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:001190">
        <property type="entry name" value="Short-chain dehydrogenase/reductase SDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF122">
        <property type="entry name" value="NAD(P)-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="282" mass="30112" checksum="3F7F5DE9D234CEF4" modified="2023-11-08" version="1">MPAPPRALDFTGDVAIVTGAGSRMNGEIGNGRAAAILLARHGAKVALVDFNVEWAQETKRMIDEEGGISEVIQADVTSEESCKNAVEKTVELWGSLHILVNIVGVGGAMGDATTINLDAWERDFRINVTSMVMMSRFAIPEMRKQGRGSIINMSSVSGCGNPSLLYPTTKGAIIQMTRAMAAHHGKENIRVNCVAPGMVYTPMTRGRGMTDEMRQARINQNLMKKEGTGWDVGHAILFLASKEAGWITGLIMPVDGGTTAGKADRPALKADTLAQDITGVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLX3</accession>
    <name>A0A9W9CLX3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_005138</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLX3"/>
    <dbReference type="OrthoDB" id="5213892at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4370617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="273" mass="31067" checksum="B3F1005840034AB9" modified="2023-11-08" version="1">MFQRGHIQDFARLGLNEQTRWILFADLPIPQIETAVAQEVAGFRFITSTIVWLDIVASITAGTAPMLVSHHSRILTAGSQTQLRDIMGCDNWVLLQIARIARLYENMNRTTQEGQDDLTGFGESASEIRLGLQYSTSQIARATSPRVTTEDPHALITRVFANMAVVYLHLVMHGFQKLDLMDDVLCSSMRIIRTRSGRHLQALVPPLFILGVIAKQEDQQFLRDILSIPPLLDPFLRHRLRLLPILEDIWKRRDTPGFAWHHCVELTKDILLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLY6</accession>
    <name>A0A9W9CLY6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLY6"/>
    <dbReference type="OrthoDB" id="5366485at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056539">
        <property type="entry name" value="NuiA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42093">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42093:SF1">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23151">
        <property type="entry name" value="NuiA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="149" mass="16264" checksum="2FB14768642BA395" modified="2023-11-08" version="1">MSSDADYASFLDKANQDTGGVEQQNSSKKSYSTKSVDTAVPKALEQVEEYYTSDADEPFEPVALKFEGSSVSAGDLKKLLGGDKEVEEVKQKSFESQYKTVIDAVKKAGDGEVKIFRVELNSTRAEYYVVAVDEKDGRLVGLKALSVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMG0</accession>
    <name>A0A9W9CMG0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005646</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMG0"/>
    <dbReference type="OrthoDB" id="3248909at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="187" mass="20878" checksum="4694D2866C4E6B0F" modified="2023-11-08" version="1">MSHSPVGLEDVASERFNIRDRDRGLNMDFMSYANLHKVRNDPTALLDTTLLLRHSEKTFQTFFKHFAVSGHWVDAGGDTESAVFEEKQGPYSSEDLWVNGTVAERIEIPSMNETATWLSLSIIFVLVVILVVLISLQIVYPKNSMQHHVECLADVLAMVAGSDELLKMSREVGIEGVERSGVQTKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMY8</accession>
    <name>A0A9W9CMY8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nitronate monooxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_005216</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMY8"/>
    <dbReference type="OrthoDB" id="2349068at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018580">
        <property type="term" value="F:nitronate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04730">
        <property type="entry name" value="NPD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004136">
        <property type="entry name" value="NMO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32332">
        <property type="entry name" value="2-NITROPROPANE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32332:SF34">
        <property type="entry name" value="2-NITROPROPANE DIOXYGENASE FAMILY, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03060">
        <property type="entry name" value="NMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4370695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="29326" checksum="0AFC4A01EB93CA89" modified="2023-11-08" version="1">MGPVQRLKKDYPWIKTPLVVGAPMRLIALADMAVEISRAGIGFINWGASLTASIPLIAAYRPAAVWFFAPSSLASLAEWATQTRAASPETKIWVQVGSVAEALDAVRNVKPDVLVVQGTDAGGHGLVRGASLIPLLPEISDALRHPGGGGDGGEGEEGEALKQDDTPVLLAAGGISDARGACAALTLGASGIVLGTRLLAATEANISRGYQAEILRAQDGGQTTVRTKVYDQLRGTNWAETHNARGVVNRSYVDAVGGMDEEENRRLYEEEMGKGMRGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN72</accession>
    <name>A0A9W9CN72_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_004663</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the epsin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN72"/>
    <dbReference type="OrthoDB" id="4033880at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16991">
        <property type="entry name" value="ENTH_Ent1_Ent2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000010">
        <property type="entry name" value="EH domain binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF110">
        <property type="entry name" value="EPSIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="ENTH" evidence="12">
        <location>
            <begin position="10"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="150"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="315"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="548"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="170"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="323"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="376"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="391"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="454"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="569"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4371446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="586" mass="65426" checksum="957195A3E59C3F18" modified="2023-11-08" version="1">MSKVVRSVKNVTKGYSQVEVKVRNATSNDPWGPVGSDMAEIAQITFNNSTDFYQVMDMLDKRLNDRGKNWRHVLKSLKVLDYCLHEGSELVVTWARKNIYIIKTLREFQHIDEDGRDVGQNVRMAAKELTSLIMDEERLRAERADRKSWKSRVTGIEEFPGGGQGPHNGEGSHRRPRDGERRQRRNEEEDLELRLALEASKNEAEEEKKRRERNQTTVADDDDLAKAIKLSKEEEELRRKELEQTNADLLFGETTAQPTTYQPTGNNQGYQQQGQVDWFGNLVDQNQQQPQNTGFLNNAYSQPTGVQGQQTGFQNGFGGYGGFPQQQQQQQPTGFDQFGGQQQQYMQPQQTIQPQQTSFNMNNPYGQLGGFGGDMGQQQQQPLQEQNTLQPGSHNPWASNKPQESLMPQPTGSNNPFAQSFNRPQPTQSFTQPTLSTLHEQKTQSQFNNTSFNPPVSFSTPQPVQPQQPQKEMDPHAARLNSLLATGEGQDTFGNVGNLRIPAQHTAPGTFVNSAGAGLNQVNANATGNNPFLNSQFTGLPQQNRIMPAQTGPAGNFGANPYGSANPFGAQQPQQRQPGSNNLIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNQ8</accession>
    <name>A0A9W9CNQ8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNQ8"/>
    <dbReference type="OrthoDB" id="2349272at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040857898" evidence="1">
        <location>
            <begin position="20"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="222" mass="22918" checksum="B3BD1B9F3F948B19" modified="2023-11-08" version="1">MGFKKTILLASALALSTFAAPLPDCPDEVEQTDSTTAITAAELITIDPTTASCSGAPFPEECADATAAATAINKSFETYDITAAGEQAALVAYMLFESGSFKYSKNHYPGRPGQGTRMMAMPPFVKLYATSVAGADAVSQADTAGGDAALTAVLALVNSDDEHSFGAAAWFMTSQCTADVRAGLVAETVDGWHNFLTACVGTTATADRDVPWVAAKQVILGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNS3</accession>
    <name>A0A9W9CNS3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">U three protein 23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_004127</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in rRNA-processing and ribosome biogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the UTP23/FCF1 family. UTP23 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNS3"/>
    <dbReference type="OrthoDB" id="25675at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09865">
        <property type="entry name" value="PIN_ScUtp23p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000006">
        <property type="entry name" value="rRNA-processing protein UTP23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006984">
        <property type="entry name" value="Fcf1/UTP23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057776">
        <property type="entry name" value="UTP23_sensor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04900">
        <property type="entry name" value="Fcf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24779">
        <property type="entry name" value="UTP23_sensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="UTP23 sensor motif region" evidence="9">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="158"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="286"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00076388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4372353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="295" mass="32778" checksum="8B8E58DF4D2BB4CA" modified="2023-11-08" version="1">MKLKRAKAYKKLLHQYELNFGFREPYQVLIDSQILQDAYKFKIDLVARLEKVLGGKVKPMITTCDMRHLYAAKPKNETLILQAKEYERRRCNHQDLEEPLSTLECLSSVVDPKDSKTNKHRYVVASNEPSVRAHMRSIAGVPLIYITKSVVLMEPMANASEEQREREEKSKFKQGLKGQRNPGAGEKRKRNDEEEGQSIADGATGDARPSKQKRQKGPKGPNPLSVKKAKKETPAASPAKKAKSTIADEAQPSTTAATDTADAEASGPRKRKRKHKSKSDGANATGDDDGEVTAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP04</accession>
    <name>A0A9W9CP04_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_004174</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP04"/>
    <dbReference type="OrthoDB" id="5551751at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040200">
        <property type="entry name" value="Mug57-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28156">
        <property type="entry name" value="FAS1 DOMAIN-CONTAINING PROTEIN YDR262W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28156:SF1">
        <property type="entry name" value="FAS1 DOMAIN-CONTAINING PROTEIN YDR262W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040945170" description="FAS1 domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="4">
        <location>
            <begin position="58"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="251"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4372400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="326" mass="35820" checksum="2844C4D82541D175" modified="2023-11-08" version="1">MKPGTFVAYPFATVCLILACSAESRPFDSIVKAFNALTEQTPLRDFNMGQPEGQVSTGVIISDVIGKTQNIAIFSGLTRDIDAVSGRLDDAAQNATVLAPDNSVMRNLKRKPWEDPEDYDSFGASAYEGSSGEDRARKNLERFVQRHIVPESPWDEGKKVKTLAGNEVWWESKDGQKKIQPGNVEVTSIADKVSNGEVWIVGSSLASRAELSISRELKLPSLPPLYQDPRQYVPATGQSWTSLRTALPTQKNNFETDSHDPESTTSPPRPDPRRTAPGLADTTTTRKQLGIGERAILLQTTHGNVLWDLIALIDQATIDFINERVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP65</accession>
    <name>A0A9W9CP65_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">homogentisate 1,2-dioxygenase</fullName>
            <ecNumber evidence="4">1.13.11.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">N0V83_003163</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the homogentisate dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.5" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP65"/>
    <dbReference type="OrthoDB" id="1689029at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004411">
        <property type="term" value="F:homogentisate 1,2-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07000">
        <property type="entry name" value="cupin_HGO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000034">
        <property type="entry name" value="Homogentisate 1,2-dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046451">
        <property type="entry name" value="HgmA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046452">
        <property type="entry name" value="HgmA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005708">
        <property type="entry name" value="Homogentis_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11056">
        <property type="entry name" value="HOMOGENTISATE 1,2-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11056:SF4">
        <property type="entry name" value="HOMOGENTISATE 1,2-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04209">
        <property type="entry name" value="HgmA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20510">
        <property type="entry name" value="HgmA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="domain" description="Homogentisate 1,2-dioxygenase N-terminal" evidence="14">
        <location>
            <begin position="38"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Homogentisate 1,2-dioxygenase C-terminal" evidence="13">
        <location>
            <begin position="308"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="319"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="377"/>
        </location>
        <ligand>
            <name>homogentisate</name>
            <dbReference type="ChEBI" id="CHEBI:16169"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>homogentisate</name>
            <dbReference type="ChEBI" id="CHEBI:16169"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605708-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605708-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF20510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4372872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="462" mass="51849" checksum="30DB9B17EC579F0E" modified="2023-11-08" version="1">MPTKLEDPKEYQKIFHWAETQKDGAIPSFGVRKNDPYEYQPGFGNHFESEAVPGTIPKGQNSPRVVRFGLYAEQMTGTAFVAPRHQNKKAWLYRARPAVAHQGFTDLPDNEDTESNYLLNNKRVHVSPTQLAWLPFDIPTEGEIDFISGLKSIAGSGDPTLREGLAVHTFLANASMKKRAAVNSDGDFLIVPQEGALDIQTEFGPLYVQPGEICVIQRGQRFKVNVEGPTRGYILEIWGSNFELPELGPLGANGLANPRDFLHPIAKYEIEKEPWEIIYKLGGKFFKEHAESFSLRCRGMAWQLRSVQFVNVGSISVDHIDPSIFCVMTAPSRDPAAPLADFLIFSPRWDVASHTYRPPYYHRNGASELMGLIYGEYAGRSDEFQPGGVSFECGFVPHGVAYEEFKAASAQPPPEMQISKVKKKHEHDPKMWDNLVDNFSEHQEEVEKILAEGVKKVNLNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPA4</accession>
    <name>A0A9W9CPA4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_003082</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPA4"/>
    <dbReference type="OrthoDB" id="722566at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045048">
        <property type="entry name" value="FBXO31/39"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10706">
        <property type="entry name" value="F-BOX FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10706:SF130">
        <property type="entry name" value="F-BOX ONLY PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12014">
        <property type="entry name" value="Cyclin_D1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="4">
        <location>
            <begin position="5"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="209"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="328"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="231"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="333"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="351"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4374341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="494" mass="55622" checksum="0A0BC58086E89BB7" modified="2023-11-08" version="1">MDTNEVSLLALPSELIHHILSHLQLSDLLTVGLVNRALKEHSTQDTLWQPFVQAQIPASTVPKPTNQTWRALFKQHHPYWFLAKRKVWFADIAHTGKLLIARYDHRINAIEAYALVAERRQPTFQTWEYNPDAIIHTFSPKVQLDLNAPVLRLNAEAYEHATGDIGYRLQKEIPMSVFNGTPRSSAGIYSRLLLSRPWPKSITTEATPVWPPLTLPSAERTRNDAPPSGYRQASSKPATLDQLSTSTFRLRKWMEFSPRPHGISMRVGEDISTWATLPEECYTPTPQKPWQGIWCGDYAGHGCEFLAIMQPDDPKPLPERAEWALRAREREGSVSSAGSWSTAPTEAALSGDEDEPQTADDLEDSVATLQVALHERESMSTEVDVMGDKDETIYRGRIEAVKLTGDPNIPRGEYTFIAPDIGPSGLLRVASEDIFKGARIVKSVGHIAANGFRDDNYMTSQLILISHDRLAQYWETFGHVSFYQRVDIDAFTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPK6</accession>
    <name>A0A9W9CPK6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_003601</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPK6"/>
    <dbReference type="OrthoDB" id="4495335at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14867">
        <property type="entry name" value="uS7_Eukaryote"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.455.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_uS7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005716">
        <property type="entry name" value="Ribosomal_uS7_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01028">
        <property type="entry name" value="uS7_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42041">
        <property type="entry name" value="DNA ENDONUCLEASE ACTIVATOR CTP1 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42041:SF1">
        <property type="entry name" value="DNA ENDONUCLEASE ACTIVATOR CTP1 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="7">
        <location>
            <begin position="806"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="305"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="424"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="534"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="615"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="78"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="207"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="340"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="439"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="471"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="543"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="560"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="577"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4373307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="951" mass="106240" checksum="412E73E5D35CAB4F" modified="2023-11-08" version="1">MSPGMPIFPVSPERVAGTKPLYGEPVQMSPSAPNLRSWSPSRHPPHRRNDSEVSVQGLAVMFESLDVKDPREAVKRLKDALDKEKVRSTEKLHKMEKEHARKEKEHEMALGRREVRIEELQSKLEAAEQRNEVGVTREQYEKERKAHKANVAQWEKVFKENEDKWRSTQAKLTESKVHGQAYEQKYREYKKKWMEVNKDALRSGAMIPTLQTKIQGLQRNVQRAESDIKFKTEEAAKYQNQVYSLQIELEGVSARLGEEIQTLKDKLRLIEGERDALKTSLKEEEVMRVAGEGHIALPTASMDEDVEFGSPVRSPRKQHTFQRVDDDKENVSPKKGAVELRFIQQELATEKRLRERAQDQIEFMKMECQFQCCSCRIAENKGKKYVHDDMYAAEMQRIKMSVPALTPPASNHEDDLMDDVIIKQEPVEDERPVTPPSEESMKEEPDDFHAEQNDNNTILVHPQDSDPEAQVTFSPSTGTFRSVPSPVKATTSIHDSTPAKPSPLGFSTVTENVPASSPWTPDAHSTMIPTEVVPAPTLRPEPETEPEAENGVEIEAGPARSDRKENKVADISIHEDAIDDSDEEDFEPPTPSHEPSGPATPYLTRTITTTTTIPLHFSPATPAFKPGRGPMTPSTVAHAAADARTPVLGELSLNKLPFDREAALEAIRERRGRARSMAAGHGTPMKQMVEGVKDRRDISAPVSRVALSGIAWTTTNSTPTSFTFTPTTATMSDGEIEVESPSGYAILPKEVTDEIGSVKLFNKARKPRTLEPHAMLTVCSGPTRSDYIQIRTPVYISHSAGRYAVKRFRKAQCPIIERLTNSLMMNGRNNGKKLMAVRIVAHAFEIIHIMTDQNPIQVAVDAIVNCGPREDSTRIGSAGTVRRQAVDVSPLRRVNQAIALLTIGAREASFRNVKSIAECLAEELINAAKGSSNSYAIKKKDELERVAKSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPX5</accession>
    <name>A0A9W9CPX5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tryptophan synthase beta chain-like PALP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPX5"/>
    <dbReference type="OrthoDB" id="10059875at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006058">
        <property type="entry name" value="PRK08206.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42937">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42937:SF1">
        <property type="entry name" value="DIAMINOPROPIONATE AMMONIA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="1">
        <location>
            <begin position="30"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="366" mass="39293" checksum="0F67A4592D8434F4" modified="2023-11-08" version="1">MFLNPLAASWRYAGPRANPAVESFHKSLPNYAVTPLISLPDLAKDLGLGYVLLKDESSRLGLPAFKILGASWAIYKAIAGQCLLPLTASIQELGAVASKNGVRLVTCTEGNWGRAVARMAKYLQITAVIFVPDFMDQATQQKIESEGAKVVVVDGDYDFSIAKAREEADKGGLLVMDVSWAGYEEIPEWVVEGYTTMLVETDRQLLELGIGAVTHAIASVGVGSWAQAVTMHYKSEERSCQATVVTVEPETAASLKTSLEAGKITPISTGHTIMNGMNCGTTSTTAWNVLKDGVDASVTVSDIDVHNDLQYLHSQIVQNGPCGAAPLTALKKLCKEKKDELGLDEKSVITLFSTEGARDYILPPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQJ2</accession>
    <name>A0A9W9CQJ2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQJ2"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="150" mass="15789" checksum="A709DB8A8F0E95BD" modified="2023-11-08" version="1">MGKVKKFIHSLRSKTSPSKGKFKSGDASSHSSEKSSSLSQWTKSYSRAINTDKTSPPAATSRTPGSGTDGGAGVTNDEDIAPPSLPSPASPSDDSHDSYRSFMNYIEALPAEQSDDSHDSYGSFMNYIGALSSQASASLISEDEFAALPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQW3</accession>
    <name>A0A9W9CQW3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_000185</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQW3"/>
    <dbReference type="OrthoDB" id="264917at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16514">
        <property type="entry name" value="RING-HC_LONFs_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327:SF42">
        <property type="entry name" value="LON PEPTIDASE N-TERMINAL DOMAIN AND RING FINGER PROTEIN C14F5.10C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327">
        <property type="entry name" value="RING FINGER PROTEIN 127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="59"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="233"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Lon N-terminal" evidence="6">
        <location>
            <begin position="318"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4377360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="565" mass="63814" checksum="FF02C4EBF4BEAA89" modified="2023-11-08" version="1">MNRQRTKGCSIVESIEQTFESMVPAKDFMLDNELPAEVQEATALDAHHDARQLVRLVQCPRCSLPLSTPVTLPCGHTVCRSCLPAPQPRSNISYPNTPDRLHGISCPLLGCGAEHAAAECSVDVTLTKLMDLIKAEIVQHKPATEDTPTLLEEILQTDKTSMDEKEVLGEKGHQQLLHGGRLVSTFTMAEMGNLRYSSELDYHIGSSDGNNYEELDVTLLERLREVTHKELDCLVCYNLMLDPTTTTCGHTFCRRCLARVMDHSSICPFCRRELHVPASLQNQSSNTILNSLLNALCPDLVTARAEALKAEEQAGDNLLSTPLFICTLSLPSMPTFLHVFEPRYRLMMRRVIEGNRQFGMVMYNRTHAAQGDLGEVQFLEYGTLLEIVNYEILRDGRSFIETRGIGRFKVKAHGMLDGYNVSRIERVEDVSLAEEAIQEQRETTMARDYAEAFFRQNPQTQLPTNVALETLSTQQLLDACTAFVREMREASAPWLRERIIQVYGEPPQDPATFPYWFASVVPIVEEEKYVLLQTTRVRERLKIVYSWIGRIRGQRWSSGNACSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRC4</accession>
    <name>A0A9W9CRC4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putrescine aminopropyltransferase</fullName>
            <ecNumber evidence="6">2.5.1.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SPE3</name>
        <name evidence="6" type="ORF">N0V83_001416</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.16" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRC4"/>
    <dbReference type="OrthoDB" id="38125at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.140.10:FF:000001">
        <property type="entry name" value="SPE3p Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000013">
        <property type="entry name" value="Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030668">
        <property type="entry name" value="Spermi_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002010">
        <property type="entry name" value="PRK00811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558:SF11">
        <property type="entry name" value="SPERMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="SPERMIDINE/SPERMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000502">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="5">
        <location>
            <begin position="11"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4376135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="292" mass="32908" checksum="B2D3EF35AB9C78A5" modified="2023-11-08" version="1">MPSIEHPTIKDGWFREISNMWPGQAMTLQVKEVLHHEKSKYQDVLIFESTDYGTVLVLDNVIQCTERDEFSYQEMITHLAMNAHPNPEKVLVIGGGDGGVLREVVKHDCVKEAVLCDIDEAVIRLSKQYLPGMSVGFEHPKTTTIVGDGFKFLEDKKGEFDVIITDSSDPEGPAEALFQKAYFELLNGALREGGVITTQGSENQWLHLSLIANLKKACKEVFPAVEYAYTTIPTYPSGQIGFMVCSKDANRDLKKPLRSWSAEEEEQLCRYYSKEIHEAAFVLPTFARKALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRL6</accession>
    <name>A0A9W9CRL6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">rRNA accumulation- protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TSR2</name>
        <name evidence="4" type="ORF">N0V83_001688</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TSR2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRL6"/>
    <dbReference type="OrthoDB" id="263560at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019398">
        <property type="entry name" value="Pre-rRNA_process_TSR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21250">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN TSR2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10273">
        <property type="entry name" value="WGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="124"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4376405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="189" mass="21191" checksum="A0C6225E0C63489E" modified="2023-11-08" version="1">MSTAAISEELQGKFDLGIWYTLFNWPDLTVAVQNQWGGPDSSDKRDWLAGQISELFASEPATDTEDVEVMLLQVLEDEFGVRLEDETEVNVAREIMAIKKEIMEGNTATVDRLQKKWEERKGKEVATGNVSVRESNQDAEWDSVDEESDEDEDQDVEMGDAPALVPAKPKPAPEVDEEGFTKVVGKKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRR6</accession>
    <name>A0A9W9CRR6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000188</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRR6"/>
    <dbReference type="OrthoDB" id="5403997at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="83" mass="9220" checksum="6894C13C3610EBD7" modified="2023-11-08" version="1">MPPKQIHGKGRTVAEPSFARNAIRTFTSPDNRSVVSAVGLFAVRHHDPFAVREDVERSALWSRWNGIGVAFLHSSWAEILLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSG2</accession>
    <name>A0A9W9CSG2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000779</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSG2"/>
    <dbReference type="OrthoDB" id="5344006at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="21230" checksum="C673EE1D8CC1D5D7" modified="2023-11-08" version="1">MDLFAVSLLTMFALTATIVLHCFYGLNPILNTVLNSTLALLWAVSFSLLSWWSSGTLKHVCNKDDWENDMGMSVCRLYKALFSFALFGLVSTLLALLLDLRVQRSATKMGRFQQLQVLGGGDGKRSGDGHAHGEYEANPNPSAARRNNVQMQRGGEGYALPEEQFAYEDDTAYQGAAGQVGRRSLEERISGQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXM3</accession>
    <name>A0A9W8XXM3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010452</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXM3"/>
    <dbReference type="OrthoDB" id="64915at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840:SF5">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase C-terminal" evidence="2">
        <location>
            <begin position="149"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="36906" checksum="0D9F707A5BA103E8" modified="2023-11-08" version="1">MPINIALVGSGIFAKEEHLPAIQATPSLSLKAVYSRSLKSAQALSEKLSDVELYSDDQDGKNFSDLLKRDDIKGVVIALPIPAQPAYIKKALAAGKHVLAEKPIAKDVATAQDLLSWTQSQSNTTATYTVAENFRFLDSFLYGSDQISSLGRILTFRVRVAAFVQPGGKYYETAWRKKPEYQGGFLLDGGVHFIAAARLLLSGGKQNMKIASAFTAQLQEHLPPVDTLNATIQLSNGSSGTLAISFGTTDTGSEYLVACEKGSVHVSRGKVTVTKVGEEAEVKEFKDEGNGVKQEIGAWAESLEKGVRNEMQSPEEALKDLKVLEACLRSGEQGGKPLEIEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY55</accession>
    <name>A0A9W8XY55_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_010013</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY55"/>
    <dbReference type="OrthoDB" id="4500315at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000396">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000009">
        <property type="entry name" value="MFS multidrug transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF4">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="518"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="561"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="606"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="633"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="671"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="702"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="140"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="69"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="355"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="410"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="86"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="104"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="424"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="442"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="459"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4362896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="739" mass="82266" checksum="B69A2B74322FE4D8" modified="2023-11-08" version="1">MVSLTPWRKTQKNAEGEHVAPAASIHEKDARQPRKWNLGILSDPQTDEVPGSVILLSRTHNRNEPLGLQHARARTSASSLPSVYGPRPRSASRGSRQSSRSRPHVPEKKRTKDGRFILDPQPEESANDPLNWRQVRRDLALISLGFYCMVGGGMTPVLAAGFNDVAATYNVTIPQVALTTGLYMLGLGIGSVVASPTAILWGKRPVYLGAIILFTVSSIWCALSPNYASLVVARIVQGFAVSPVECLPSATIAEIFFLHERAYRLGIYTLLLLGGKNLIPLVSAAIVQSLEWRWVFWITAIVVGFCFFLIFFFVPETFWDRAPRPAKRHTHNRSQSPGKHHMHLHLPLRHAKKSTGELDTQFQTPGADASMPSSPLEHPVKRTRTAHVGFADDQAPPATTAHVDFARGSQHQVDMPPPLHSPISVHSTPPGSSRQDYFTPSRLDDTTDPEKQDSRPDSRSLSTDQPTAIDSDHISEQFSIHYTDYYRDAPAKSYRQSLKPWNGRLVKDKWLRVMIRPFILFAYPAVLWSSLVYALAVGWLIVLSESVAHIYRDESYNFTPLQVGLVYISPFVGGVLGTAVAGKVSDLVVRYMARKNDGVYEPEFRLLMAVPISISTVIGLMGFGWSAEERDAWIVPTVFFGVISFGCSLASTTAITFVVDSYRMYAGEALVTLNFSKNILHGFIFSLFFTHWLEASGSKTTFLAVGGIQLACMLFSIPMYIFGKRARMWTVRKNFMEKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYA5</accession>
    <name>A0A9W8XYA5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010147</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYA5"/>
    <dbReference type="OrthoDB" id="3755781at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="479"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="520"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="259"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="354"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="558" mass="61840" checksum="D9844B242947C168" modified="2023-11-08" version="1">MHFRRHSPTRLHPVASPTDSINAQSPPKTSTRTQKTLEEILALSASLTKPLPQEPQNVTNHYHFSSSPQSDRSSEQHRSPTREPRSKKTGHATIHTDATATQAEFESDAFAVHMPTTRLPIMDRTTSPTKTSPAAQAAAYQTYKEKARQVRERNNSQGVRVPSKIVSYDCASPTTAGHRAPLVKENDPLKNLPTPAGSFPISPPLPQTGWTRPQPIYRAHAQGSRGISPPRKTQTPRKTVAIASTARVTNKWYTIQSDSEAGASCSSSSPSSSPTRQPRQPAIKVRVKPRVLVPEAERVQTESRYSFYNRPSLVSSPPTSRSPSPVKSMPNFTRHNSIEGDSIFGYRSKDIGGAVAGASSTSGSDKEKEKRRTPEKPKKTVPKRTLTSRFPWLKPSGPRIAKPVTAPAISTAATTTQTQTQTRGYVDPFVRHATPPAPTSPVLRTPIAIRPASPRKFAPETAVPESQGKFDSGFAQIKGLTFLLLKIGFFLYGVVALWYVLDAIREVFNTLSAPFRAVKWAWGFVWLLCLWIMRIGIIMWDKWVFKVLLQSGWMWKMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYG8</accession>
    <name>A0A9W8XYG8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8035 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010196</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYG8"/>
    <dbReference type="OrthoDB" id="2192830at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058348">
        <property type="entry name" value="DUF8035"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26118">
        <property type="entry name" value="DUF8035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8035" evidence="2">
        <location>
            <begin position="280"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="256"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="508" mass="59207" checksum="1E3F562DB632AC7E" modified="2023-11-08" version="1">MYRASTGHLDHYDEPPPPREPPRGGERWDRDRFERMRRGAGGGGGSQSGGGSIAGGSRAGGRDEYEHFRFQEHDRFPGGGRDIDIHEDRQRRGPPVMERERFREEERFDRPSRSRTELFHEPTPSEVANQALAPYRRKSVIDNDINIGIDIKEKRRPARPQYIRRQSSLDTFDRRPLPRYGDIEKFERHEYRPPANVPIPLPIRERRRSPPRRFHEEDDFEEIRFDDRGGRGRERENYREVEVSREKSRVRRSKSVAKSTRSSSISSFEEIQPPRATWGKKGKTRLPKRLVKKQAVIDLGYPFEEEDDFIIVTRALEKEHIDEVIKISESYKEEKTTYVYEEKSEAPPPPPASIAPPPPPPQEHFAPPPPSVMHAPPPPPPPAQYAPPPPQYAPPPPSHYAPSHYAPSERAPSPPANYEHERFVEIERSNAMHGPATSFLPEGRQIVRRRSERDIRDEIRSLEEERRMLKYERDERDYEFIEARPSRREVVRVDKDRKGRLALVRSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYK2</accession>
    <name>A0A9W8XYK2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYK2"/>
    <dbReference type="OrthoDB" id="5317787at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="466" mass="53461" checksum="DA35F7C1078C3E76" modified="2023-11-08" version="1">MSRNVHWRQNQSSSTTSGRSDNSSRSYDTAPTEYSDRRPSLVHSATCYARVEGRGQERDYFAAKDDRQRYDNPPPRVSVDTYASTSDEGVADDHDDMPDYDVPQSHYEPLPCEAIPTTPRDFGELFPTAKRISIRHDDATIDGNMNLRVDTQIEERGHRKQNYTLFHLRMQDLRTREFSLRRYCRESGREVCHSMRKYQKPLSEKRPVLQRASTALANLVKTDSRPSTSSGLKRADSGYESVRGGVDVDVEDDDDDDESEPRPKPVGYSKGRALIPTNTIKLEFSNYAHMDVKRRGAKSHKRYAFEYWGHDYAWKRIVKSESQRDQESISYYLVKDDNDREPLAHICPVKLTQEEAREEAARGGWVPPCYMRITGDSILQSDTDISDVVIATGLMALVDDCIKRRFHSKQSTQLHIPLLRSSSLMMNMEYIGPKRLIDEVFHRKHHDGGSPKKQPPSALRRVPTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ41</accession>
    <name>A0A9W8XZ41_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Developmental regulatory protein wetA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_010811</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the wetA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ41"/>
    <dbReference type="OrthoDB" id="2575228at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048315">
        <property type="term" value="P:conidium formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040112">
        <property type="entry name" value="WetA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22934:SF25">
        <property type="entry name" value="DEVELOPMENTAL REGULATORY PROTEIN WETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22934">
        <property type="entry name" value="PROTEIN ESC1/WETA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0183">Conidiation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0749">Sporulation</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="234"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="415"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="415"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="445"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="464"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4361870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="561" mass="61089" checksum="3F2148B517DC34CD" modified="2023-11-08" version="1">MASLLQQASDIPMRPIRNKDMEVVDLDQLFEDYGETDLLHHFTETTADRSSSDDLAHLFELPSSNESDLFGTESILDREAEASWHEAPRKCDQNPASSMLDDSSSFYIDSKGKASLSDSELLSFEDLFELEQNQLRSISQPSTPRPHTTTRSVKKAVSFHDRSVPRGVQKSLKKSPATSFAKMMQPMYYRSPIPDVWTRKMDAPADASYLRRPSNGISSPPLSSKIVQHENGNGFFAQDHHQPYNTRSSRSNEEPHTPGIDFSNYQLTPQASPGMVPSNGSNPFDDHMGMTFSSSSVSSAALSALQTPPSSLRIPMTTWGTDTSPSLDFGFSASPDFSATSKTAGWWNDDAAAQGSAGFRDSHSRSSSQTLGLNGLGISCDTASFGQFGSVGLGVSMGENVPGASASSFDVGSYNTMYPTPPQQHIVSIGHRPLSRSPSPCPQPRFHRRRPSSHSHAPHHRASHSSTASSRRKSSQSSAHSSRQSSNGNVGFVNFTPDDSRKILTGVAPSGSSKTKARREKEAAEKRRKLSQAAMKAVMEAGGDVDSLRRLEREGLLVLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZD2</accession>
    <name>A0A9W8XZD2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_010977</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZD2"/>
    <dbReference type="OrthoDB" id="10009520at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26200">
        <property type="entry name" value="Rcat_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="132"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="20"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="377"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="572"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="654"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="52"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="412"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="593"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4362035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="674" mass="73810" checksum="671210130A94F4A3" modified="2023-11-08" version="1">MGSKISKAVRPHVKAAALPVATARNDANEERARQEPVLQLPDKYSNDDINIATSTTASTTSQRRNSEPARPAAAASSANMEEAANSFSLFLEELRLRAIESGAAHRTPPSPRVGLPPPPPPAAWAPPPPGEAEVRCMICCAELPKEEDPNYTKEAIKPCRCSGVWCTSCIKALFRDACKDLTLMPPRCCVPIHLHYARPYLSDAEIAEFKSKYDEWSTPHPFYCPVPTCSVFIPERLLPQQAKSNSKRTDSGIGTPNAATFGCPTCQATICAGCRQVAHPNSICNVEEFGIDAATAALLKSWGYKKCPRCGHGVKRMFGCNHMECRCGAHFCWVCMQHRDQCDGQCYEEGEDEYYSDDEPDEPDQSVAQPNEIGNTEQNEAAGADAEASVEQATSTATHPQPVVRPQNLDGGGDRYWERQDIDFGPEPTDDLQDRAWQCVHNFGTYKISLATALTSTPKVECTKCWCTIRPEIKASQSQHQASDKMVPASAGARPRGIQRVRVRDVGRYLPPRGLFRADASVGTAPHLMAPVSPLSQSVPTRETSPMEDVQYTSSDRVFDTYGNIIATTELQEQRRSSLDSPSPQALAVRGKQAPDKTSLEKHKSTVKSFSEVFATLPPPTKTFSLAYECPYCNLLVCEACKDAIVHAQKNDPATANEASMLNRDSEPSIVPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZY0</accession>
    <name>A0A9W8XZY0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cyclopentanone 1,2-monooxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010014</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-binding monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZY0"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050775">
        <property type="entry name" value="FAD-binding_Monooxygenases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43098">
        <property type="entry name" value="L-ORNITHINE N(5)-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43098:SF3">
        <property type="entry name" value="L-ORNITHINE N(5)-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4362897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="553" mass="62331" checksum="6AEC20378E803F04" modified="2023-11-08" version="1">MGSTDELNMNGLSIGTNGTDVQELDALVVGAGFGGVYQLKVIRDAGYKVKLVEHGSDYGGVWYWNRYPGARVDSSIPHYEFSDPALWKDWTWNQRFPGSAELRTYFAHVADKWDLRKDTQFNTFVSSATWDDATARWTVKTKAGETYRVKFLLLNTGFAAKRYIPDWKGIDSFKGTFLHPSYWPHEEPDLKGKRVAVIGTGSTGIQLAQELSEVAGQLTVFQRTPNMSLPMAQVDYELPKQALPKPDYLNLFAGRTDSFSGFSFNFIPRSTFDDTPEQRRKTYEELWSEGDFKFWLATYYDMLFTKEANREAYNFWCEKTRAKVNDPKVADILAPIEQPHAFGCKRISLENGYFEIYNKPNVSLVDISDKGTPIQSITEKGIKTTDNEYEFDFIISATGYDAITGGLRQIDIKGPSGESLSEHWKDGAKTYLGLAVSGFPNMFFTYGPQAPTALCNGPTCAELQGNWILQAMNHMKEKSLSKMDAQKESEDEYKELIWKLANASLLPSVDSWYMGTNIPGKPREPLIYLGGVPTYYKTINETAANGYTGFTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZY4</accession>
    <name>A0A9W8XZY4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010735</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZY4"/>
    <dbReference type="OrthoDB" id="3801471at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="221"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="362" mass="39695" checksum="93D7EDA5ABD11200" modified="2023-11-08" version="1">MSTHFRPRPPTATPSSPTRPSTARSTVRAVTPSPSDSFNKVFRMQFPSSPMHGKLKSESYAPVHNEDFSGSNHSKIDEEEVGRYILGANDVLGPGLQRGGLASGRSSPMKARSRSQSPKKRPYSTPTELRRRPDLSAQHRDPREELEKQGEAGGNEDAEGSVVDQEETGDWPSTPTPVSRSQRQTAYMPLGTPLRLFKQELEPDHENTRARPRQVTANASTTQQTPPFTLPQPQQSPTHLTPALPLNQPLPRKQHATNDPSRHVSVSALSNMSGGTATSKRSIFSTPGRDELERKKALVEADEGPFARAVSMQDLQQRRRRVSEGNAVAVVSGGGEGQEGKGARVKKRGCGWVVEREWCSVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y054</accession>
    <name>A0A9W8Y054_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alpha-1,3-glucanase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y054"/>
    <dbReference type="OrthoDB" id="3257981at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051118">
        <property type="term" value="F:glucan endo-1,3-alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11577">
        <property type="entry name" value="GH71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005197">
        <property type="entry name" value="Glyco_hydro_71"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03659">
        <property type="entry name" value="Glyco_hydro_71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040922626" description="Alpha-1,3-glucanase" evidence="1">
        <location>
            <begin position="22"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="470" mass="50904" checksum="7AEA7375D16FC2DE" modified="2023-11-08" version="1">MHSVFTLAIGLLAALSPASLAQPIEERQATDRYVFAHYMMGIVQHRTSSDEFDQEFGLAKAAGIDAFALNFGPDVSDTILGYAYDSADRVGIKVFFSFDFNSGLYSTSDPATVGNRIKAFKDKPAQLIVDNKPFVSTFAGPGLDVAAVESAAGADIFFLPNWYPGNDQTGTDGFFNWMGWYSDGNNNPGTIPPSDGDTAYINTVGSKERYMAPVSPWFSTHYGDWVSYSKNWVFQSNDLWFTRWNEILTLGPRFIEIVTWNDYGESAYVGPLSAQHDDDGHSKWVNDMPHGGWLDMAKPYIAAYKAGATAPTISEEKLIYWYRPTPKGITCSGDNLGKPPGWDLMTDEVFVVALLQTAGTVVATSGTNTQEFDAPAGASLYKLPLAVGTQKFTLKRGTTEVMSDTSLRDIVDTCACGIYNFNAYVGTVPAAAPDALVNFDAIASNVPAGYSCQPTPSLPIRASGAQATST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y098</accession>
    <name>A0A9W8Y098_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Histidine acid phosphatase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008998</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y098"/>
    <dbReference type="OrthoDB" id="6509975at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000065">
        <property type="entry name" value="Similar to histidine acid phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016274">
        <property type="entry name" value="Histidine_acid_Pase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963:SF12">
        <property type="entry name" value="HISTIDINE ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000894">
        <property type="entry name" value="Acid_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="117"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="301"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="474"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000894-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="515" mass="57729" checksum="7FAACC7526C4E896" modified="2023-11-08" version="1">MAIQVSTSITGKYSPQQQFSNEDAWLLLRRHEGPYGNDYVRGHFIVVHGARCGNGTIGDVGTDEVDESLAYHDAQIPLQPLDHSQGYQFDPLLHLPGISPYFDAVGFGLEHKAPPGCNVTAASYIVRHGAIYANDKEYEEYIKPFLWKLEKQRQGWSGPLSFMEKWQSPILEDKLEDLTPSGAVDAKAVGKHLLERYPDLVPETRRILADKKSRTYDTAENFVKGFPQVDDIEIVRVTENINGSMEAIIPHKSCKNFSKEPGTKEQHAVIDLYGASVARRLAPYTPFTLSPTDIVGFQMLCGYESAITGSRSPICALFTDAEWMAYEYAWDLKYAYMVGPLNPLSPYLGFPWLQAQAELFTHIDEHGNPGEAWPEKQRFFLSFTHREVPPFVATALGLFNSSSDAREQFPTDHINWTRAWKMADLIPFLGHVGMEKMTCERGGVGSGDDGDDEEEEKVFIRFIANTAPRPIPNCQDGPGASCPFGKFRDLIGEGAKQHEDFHRVCDKKDKKDGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0B4</accession>
    <name>A0A9W8Y0B4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Nitrite reductase [NAD(P)H]</fullName>
            <ecNumber evidence="19">1.7.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">N0V83_009028</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>NH4(+) + 3 NAD(+) + 2 H2O = nitrite + 3 NADH + 5 H(+)</text>
            <dbReference type="Rhea" id="RHEA:24628"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16301"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.7.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>NH4(+) + 3 NADP(+) + 2 H2O = nitrite + 3 NADPH + 5 H(+)</text>
            <dbReference type="Rhea" id="RHEA:24632"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16301"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.7.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>siroheme</name>
            <dbReference type="ChEBI" id="CHEBI:60052"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Nitrogen metabolism; nitrate reduction (assimilation).</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.7.1.4" evidence="19"/>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0B4"/>
    <dbReference type="OrthoDB" id="432169at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008942">
        <property type="term" value="F:nitrite reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015980">
        <property type="term" value="P:energy derivation by oxidation of organic compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19944">
        <property type="entry name" value="NirB_Fer2_BFD-like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03529">
        <property type="entry name" value="Rieske_NirD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.413.10:FF:000007">
        <property type="entry name" value="Nitrite reductase [NAD(P)H] large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1100:FF:000002">
        <property type="entry name" value="Nitrite reductase large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.480.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1100">
        <property type="entry name" value="BFD-like [2Fe-2S]-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="entry name" value="Sulfite Reductase Hemoprotein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007419">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041854">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052034">
        <property type="entry name" value="NasD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006066">
        <property type="entry name" value="NO2/SO3_Rdtase_FeS/sirohaem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012748">
        <property type="entry name" value="Rieske-like_NirD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02378">
        <property type="entry name" value="nirD_assim_sml"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43809">
        <property type="entry name" value="NITRITE REDUCTASE (NADH) LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43809:SF1">
        <property type="entry name" value="NITRITE REDUCTASE (NADH) LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04324">
        <property type="entry name" value="Fer2_BFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00397">
        <property type="entry name" value="SIROHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="Nitrite and sulphite reductase 4Fe-4S domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="Nitrite/Sulfite reductase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00365">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="22">
        <location>
            <begin position="977"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="425"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="425"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00066907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00070300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ4364434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1162" mass="128206" checksum="8B89F5E9A0CA00A6" modified="2023-11-08" version="1">MAETEPIVTHGIEPSSGESHSPHDEKPGVQATWGSKDGNISNSEWNDAEANKLPEKVEEMEKKGEIPNDNGPRKRVVVVGLGMVGIAFIEKLMKYDVKRREYDVVVIGEEPHLAYNRVGLTSFFQHRQVENLYLNPKEWYSSMPEGSLNYHLNTLVTEINPDDKTVKTSSGETVSYDILVLATGSDALLPRHTPGHDAKGVFVYRTIEDLQKLIDFSASRKGTTGAVVGGGLLGLEAAHAMMDLEDFAKVKLIERNRWVLSRQLDGDAGGMVVEQVRALGLDVMLSKRVGKILTDEENSVKGVVFEDGEEMECSCICFAIGIKSRDELARKAGIKCADRGGGIVVGPDLITSKPDIYAVGECASWENQTFGLIAPGVEMADVLAFNLTQAKAHAPRKFKRPDLSTKLKLLGVEVASFGDFFADRDGPKDMPGRARAEKKGAEGANGTTPRDRVKNLTDGPAPPPVKALTYKDPFQQVYKKYLFTMDGKYLLGGMMIGDTKDYVKLVPLVKNQKQLEMPPSELIVGASKGDDDGDDLTDDTQICSCHNVTKGDVVNSVKDGTCKSIGDVKSCTKAGTGCGGCMPLVQTIFNKTMASMGNEVKNHLCPHFEYSRADLFNIIYVKGLKDFSAVMKEAGKHPDSLGCEACKPTIGSIIASLFNKHLLDLDRRGLQDTNDRFLANIQRNGTFSVIPRVSGGEITPEKLIIIGMVAKKYNLYTKITGGQRIDMFGARKQDLPDIWQELIDGGMESGHAYAKSLRTVKSCVGTTWCRFGIGDSVGMAVRLEERYKSIRGPHKIKGGVSGCVRECAEAQNKDFGLIATEKGFNIFVGGNGGAKPRHSELLAKDVPPDDVVPILDRYLSFYIRTADKLQRTARWMENLPGGIKYLQEVILEDKLGICADLEKQMEDLVGTFFCEWTEVLKDPVKRSWFSQFANTSDNINDTIEPITERNQQRPSYWPKDSVTEDFRGHKWESELSWQPLVQASEFKDADTGDSKAIKRGDTQLAIFKVRGKFYCTQQMCPHKRAFVLSDGLIGDDIANNKLWVSCPYHKRNYELSGTNAGKCANDESVNIAVFPIEERDDGYLYVKLPSVEELDSVLGTEKFKIKKSEAEDPFVALDKKLAKMQLKGRKGMQVSHLEGGWGEGEGGDDSCGWGEGCWGLDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0C6</accession>
    <name>A0A9W8Y0C6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009977</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0C6"/>
    <dbReference type="OrthoDB" id="4454541at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4363680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="278" mass="31737" checksum="7FFF39F7562B5BBA" modified="2023-11-08" version="1">MELNFIRAEAYNKIVAQVGDPLLPQDESTLRRTVEDTTVELSLWLEEWTVIVSTEPTPQQQLLALQNLHIQREWAMMTLYLKAIATSGIENIAFMTNFQKEFVCRAKEAAARHLHHVLEDSSTPFSSPALSSEHTRSTYLSTFKWTLDYVWAKCAFSILLVLKLAILLRDPVPSVMLLLRDAHRVLEELKTVTVGHIAYFQILQTSIEKCEATLREYVTQQNAGADLDATSNSNGVAEDEFQGYVPSEFVFEWDFPGLNLKHMPLGWQDLFINIDNIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0W7</accession>
    <name>A0A9W8Y0W7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_009605</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0W7"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000078">
        <property type="entry name" value="MFS maltose transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF77">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="317"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="352"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="386"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4364150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="458" mass="50224" checksum="035C9B767E8AD55F" modified="2023-11-08" version="1">MSTFIRHFGQQQSDGTYAISASDLSIMSSMINVGELVGSLGAAPLNDFLGRKGAFLVAAIVAIIGTVLQTITDHDKGIIIGGRVILGLGEVAPTAMRGPLLGCWQLTISLAQIIAAVVNRGTESMTTTASYRIPTALQLIFPLTVVAFLYWVPESPRWLMRRGKTERCDAALRTLHKDDKAYTAEEDIRMIENSLAAEAEQSIESSWLDLVRDPVERRKVVFSAGALIAQQVNGIQWFYYFGTVFSKAIGLNDPFLMSIIVFVIQVVTVFVAVLCANTIPRRPLLLTTTLMMMGSIFIVGCLGIPGGDVSSTAGKVIISFVIIEITAFNFAWGPLGWTIASEMAVGRNRNKIYAVAVACFWVTVWATVFTLPYLYYSANLGPKTGFVYTGLCFITLAYVWFCVGEVTGRSMEEIEWFFREGIPARHWKRQPRLGSGSVVGAENVSLEKGKVDHEKETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1L6</accession>
    <name>A0A9W8Y1L6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_010246</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1L6"/>
    <dbReference type="OrthoDB" id="1368at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044669">
        <property type="entry name" value="YneE/VCCN1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33281">
        <property type="entry name" value="UPF0187 PROTEIN YNEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33281:SF19">
        <property type="entry name" value="VOLTAGE-DEPENDENT ANION CHANNEL-FORMING PROTEIN YNEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25539">
        <property type="entry name" value="Bestrophin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="457"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="45"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="471"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4363126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="483" mass="54802" checksum="BE0BBF1FAE4CFDBB" modified="2023-11-08" version="1">MASTTSTTKTLKPDAIEIPSTSGTVKNEEESDGPMSPRSWRHRTVTYQPPSRRQTTGSLDAEDYFVGPRDMSKHSKWPFFMRMHGSVFPKMVLPLTVVTVWSTAITCISEFVYPLAVSTLLLTVLGFVVGLAISFRTSTAYERYTEGRKYWTQLILVSQNLARTIWIHTAEREGDLGKEDLLNKLSALNLLNAFACSVKHRLRFEPGIDYPDLRDRVEFLDTFAKAAELDIPPPLEKNKAKAVGEYLGVTFAESNPRKRIKRSKKPLGNLSLEILNHLSCYVHSIIDNETLKIGLYQNQAITGIFQLNDALTGMDRVLQTPLPIAYSIAISQITWVYVMMLPFQLWDDLRWITIPGCIFAAYIIIGLAAIGREIENPFGHDVNDLNLEAYCEELEMDIDTITSQPAPMTREFMRRPGNMPIWPLSHKNYESWVGRSKQDIRDALMTKTKADMLVRKSFAQPRQSEADEKTNATLQQPHHQQDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1P0</accession>
    <name>A0A9W8Y1P0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Splicing factor Cactin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_009574</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CACTIN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1P0"/>
    <dbReference type="OrthoDB" id="265955at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019134">
        <property type="entry name" value="Cactin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018816">
        <property type="entry name" value="Cactin_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21737">
        <property type="entry name" value="POLYGLUTAMINE BINDING PROTEIN 1/MARVEL MEMBRANE-ASSOCIATING DOMAIN CONTAINING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21737:SF4">
        <property type="entry name" value="SPLICING FACTOR CACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10312">
        <property type="entry name" value="Cactin_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09732">
        <property type="entry name" value="CactinC_cactus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01050">
        <property type="entry name" value="CactinC_cactus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Splicing factor cactin central" evidence="5">
        <location>
            <begin position="44"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Splicing factor Cactin C-terminal" evidence="4">
        <location>
            <begin position="394"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="316"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="326"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4364119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="553" mass="63695" checksum="701D549511B681D0" modified="2023-11-08" version="1">MASREPPWRNERSRLPQRSAPTSTIPAKRSADPRAHEHPAKRSTNAAEEAWVADEDRFALQQAKKKAALRVKGGRAKPIDWLAVTLRFIDPTEKNILDEEVEDHELDVVDPEGVLEGLDTSELLELEKEIESYLTLETNRSNRDYWSSLKAICKDVRRKARSSAADARGASSVSADIDQLLAPKSYEQLETLEVQVKRKLDSDEPIDYDYWEQLLRSLRVWKAKAKLRRVSQEIVTERLQALRRQQAEEAASVQGKIYDMVKPIDESTQGQSTMVAYDAALDPQPLLKLRAEDKSLPQIEEKAFLGNVANERRRIQKLGYVPSQTKSEDRTRSESRPKASQAITTSASRFAPVEKEDYSKATMALYEREVARGVEEGEEIFTAEEEVTTARPQWAGSYKPRKPRYFNRVQMGYEWNKYNQTHYDHDNPPPKVVQGYKFNIFYPDLIDKAKAPTYKIERENGRKRGQSFAAAGEDDTCLIRFIAGPPYEDIAFRIIDKEWDYSAKRERGFRSSFDKAAKLIGIWHVQTTREKVTDLLQGILQLHFQFKKIYYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y210</accession>
    <name>A0A9W8Y210_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009137</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y210"/>
    <dbReference type="OrthoDB" id="408373at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF33">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G14860)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040759128" description="AB hydrolase-1 domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="49"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="220" mass="24415" checksum="1E02F82785B68CA3" modified="2023-11-08" version="1">MKFLSFLSTFVLSLAQVALGELSNFDDWEHERIQLEDVSIHFRYHGQGPPLLLVHGFPQHSLTWHTIGPILAQNYTVIVPDIRGCGDSSLSLTKNYTAAAGGTDLKAILDYLNITETYVFAHDKGVGLASSLAIEHPQAVKRIILAEYPLPGFGYTTTVTSPNAYLNWQLAFFAVPDAAEYENPTWTANRALRFFEDGEHISSVDLSYLKDRVTLQGGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y233</accession>
    <name>A0A9W8Y233_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009813</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y233"/>
    <dbReference type="OrthoDB" id="1933717at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF8">
        <property type="entry name" value="BENZIL REDUCTASE ((S)-BENZOIN FORMING) IRC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="28768" checksum="208FDA1353C9C666" modified="2023-11-08" version="1">MADKLIVLITGGNTGIGYESVKALYASPQAHIILMGSRSLEKAHIAISTLKSEVTDSKSEVIPLQIDLEDDTSIDNVYKEVESKYGRVDALVNNAGGSFDSVIASKPGAAGIREGWNHTYDLNVTSTQVFTTKFAPLLVASSHPRLLFVTSGLSSLATAGTFTNKVISGPPPKGWPKPAALSMLAYRSSKSALNMLMIDWQRQLENDDAKVFCISPGFLATNLGGMGAQFLKSRGAGDPSQGGNLIRDVVQGLRDEDAGKVVNKDGVQPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y279</accession>
    <name>A0A9W8Y279_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009501</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y279"/>
    <dbReference type="OrthoDB" id="2123952at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF18">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="2">
        <location>
            <begin position="38"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="417" mass="46850" checksum="48CDEAF43F6B6A13" modified="2023-11-08" version="1">MAACALSSARVTDNALFNPAWDKQSLISTTSEVFHHAAINALPKMENPNQSLDLMRAYALLSLVAMQHGNTRDMQAFLGKYHALVAMDGLHDEANWPKDLTIIELEERRRLYWSMYTLDIFSCIVFNSLIRTREEQSNVRYPTELDDAFFDNSGYRGEPQSPASTASPSSNVSPESWLHGYNITTDLWRLLEHVTIKLHSHKKKKRTFLQVATNFETSPSAGTLQAEADRIYFNLPHHFVIINEMTGDQGRDLYGFQAANITATAQLLRMVLLASEQNTVEQRCQVVSEVVDAFMQIPSSYLRAISCPLLHHLGGIGSVLGSVLEESLSSYQYQQVRTVLLSLAQLLENLDLGMHSVQTADKLRGLVIQIDEHMASQVGNESSTSTTFLGMRNRDHSPQNFLTEWSWKTGFMQSSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2B0</accession>
    <name>A0A9W8Y2B0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2B0"/>
    <dbReference type="OrthoDB" id="5086500at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046536">
        <property type="entry name" value="DUF6601"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34414">
        <property type="entry name" value="HET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34414:SF1">
        <property type="entry name" value="SUBTILISIN-LIKE SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20246">
        <property type="entry name" value="DUF6601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="369" mass="42606" checksum="69EAF77177CA74DB" modified="2023-11-08" version="1">MVDEDPITPASTLPACVASFSRSSTAADKAWDSTVPPFPQSNQLYEELHYSQGPEKPFCLRSHGSRQTLPDAPRVRLECPSQIISHCLDDLDTPQMNKLGEKLWRAGPHPEILSLTQQLSMDRRIVVNEDPSVHLLWAAVDGILYVKPLPAYLTSFAFWRYLLDTSNSDVNLEERERIRVTALGFLKTYSSLIQRRSDFNLARRHDLLASFDNISFETFITFISHFDAVPQSAISARWRYGILQLDALNFHSILQLRRWHFNRFESRYGAYFQRLFPIILFIFALLSVILSAMQVILGARQIWDTDNKGLKKTLEVFEWSATEAIAWSLAFGLMFVVWWIGVSTAEVCKRRRMQRRFCKRLKEEGVDRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2K1</accession>
    <name>A0A9W8Y2K1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">mRNA decay protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">NMD2</name>
        <name evidence="5" type="ORF">N0V83_008997</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2K1"/>
    <dbReference type="OrthoDB" id="27832at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000052">
        <property type="entry name" value="Nonsense-mediated mRNA decay factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000037">
        <property type="entry name" value="Nonsense-mediated mRNA decay factor (Upf2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.80.160:FF:000001">
        <property type="entry name" value="Nonsense-mediated mRNA decay factor (Upf2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.80.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039762">
        <property type="entry name" value="Nmd2/UPF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007193">
        <property type="entry name" value="Upf2/Nmd2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 2 UP-FRAMESHIFT SUPPRESSOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839:SF7">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04050">
        <property type="entry name" value="Upf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIF4G" evidence="4">
        <location>
            <begin position="472"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="4">
        <location>
            <begin position="677"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="171"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="242"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="909"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1025"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="171"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="918"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="951"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1058"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1080"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1110"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1123"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1175"/>
            <end position="1193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4364403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1193" mass="135353" checksum="8C4623786F2C1DB1" modified="2023-11-08" version="1">MERQRKRELRELNIRAWDGEQDVFPVQGSLDSSMKKNTAFIKRLRTAVNAPAQAQFLQEIRTLSLHKYLSEVISACYEGLCKLKTPGEIAAGVEIVSALHQRFGPAEFTGYIAWYIGRGLATPDKSHLKSLAQDVREREEKERLARQRVLLRVATELWLVHVLRSLDDVARPEEASKSKDNGKLVEGSAKTRARADTADAEPFPLEVLKDMLGHDRDHVNLPLVVIFVKAFAWDVLGAKTSSTEGRKTVNEDGTTTTDRNEDTSASENEDQASKDAPIISPELQQRFKNILTRYFEDVKAHLLRDQKHLLNQGRKNAEAYVKSGEVFEDRQSNYEKQMKSQERLVANAQVLADALGVDMPDLKEKDSTSNAGDSAIGLVKAGEYLRGQSDGAGIWEDEDERRFYENLMDLKERVPGILLEEPKKKKTDGDEQVGKKIEAKVEESVDTAADAKAAEADDQSTAIANKSVGAQVDAVLARLPELSTKDAVDQAAMDFCFLNSKASRNRLIKAVQEIPKGRSDLLPLYSRLIATLGKYMPDVSQGLVSYLDDEFRSLQRRKSKDFLGQVRTQNVRYLAELTKFGVVPEHVIFHCLRVSLDDFSRMNIEIICNLLENCGRYLLRNPETSPRMASFLETLQRKKGAQVIGQQERMLIENAVYYVNPPERAAIEQKERTPIELFLRKIMYQDLTRRTLDKTVKQIRKMHWEEDDIVNLLHKVFAKPGKIKYSHIHLLAVILGTIHRYHQDFAISVIDDLLESITFGLELNDFKFNQRRIAEVKYLGELYIYRLVDSPLVFDTLYKLLNYGWEGGYARPGLYNPLDNSDDYFRIRLVCSLLETCGMYYDKGAAKKKLDFFLTYFQYYVCVKDALPMDVEFIVQDAYNLTRPQWKLITDLDEASHAFAEAVKQNYQTTAADKPAEPDEDDLSASDDELEGPEDDDVVMPEGEGDKSSAEEDEDTDDDEPMKHASSDEEEEQIIVTRQEDERDPEAEADFDRELAKLMSESAESRKFERKPVFDVPLPMRRAREATANPDDSGNEAPAPVMPSHTMKFSLLSKRGNRTQTRSIDLPSDSTFAVAMRNKQQADKEEQQRIKSLVLNYNEARDDGDDTGDSPFSYTLRPNTNRKHTETHKGLDKTPNPYAQPRLDKAGNNRSNQRARKLQLSDVNWYDHPSHMSSRRTGSGSGATSSTVSTARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3L1</accession>
    <name>A0A9W8Y3L1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Spindle pole body-associated protein cut12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_007561</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3L1"/>
    <dbReference type="OrthoDB" id="5383703at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021589">
        <property type="entry name" value="Cut12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11500">
        <property type="entry name" value="Cut12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spindle pole body-associated protein cut12" evidence="3">
        <location>
            <begin position="188"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="94"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="365"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="413"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="495"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="572"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="225"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="383"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="417"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="443"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="454"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="512"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="572"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="596"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4367031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="646" mass="71225" checksum="3D43B6E6085E9D7C" modified="2023-11-08" version="1">MFSWITGPRITNVIEDLQPGRLPEAPVHTMPVLTLAETNYESTFIEPPETPAHQFAVNAFKHAIFGTPAVDDTNAASNKSTRKAKLDAANAKAIELPAPKEDGPPSSPSKQAGGILMTPGTANKGRKTVSFGSHVVDNEGKRGNTGKSGIPKDCPGKFPSPWTPGTQLKLDPSSDKRPRTKLTEALLDARTTIQTKSGQKPKARDDSDITMDLGAPRSESGKYWKEQYESYAERSEKEMKKVIAKQQLAKKFAMKKDGEVTELTTKLEQERKRFRQRERELEQQNKDYQERLRQTMADSMSATIEIAALKARVATLESPNVIALNFQQSKTPFEIYEDTSREASQLKTNPEMAADVSLGASISMASSGKENASPKARRHHRQSLPSTSTRPNIPSRLGTAGDEASIILGRSPRALEQSSGAASDPAQAPQSSDQHLKSPLRIRRTETRNENSEPKSPAIILPSSPLPMPSPDPWLENDASIPQLDRLAMPISTGQPYSRPAWNPPPRHQRATKSVSHSRPAQVSKNDRHVEKTVPTVEEVGRSKVRSNTMEQPVLPVESKTTLAASRINHKSSIQSAKPEATRNTEASVDYSPTDPKFDMSKVTSHHAEGSVHVKHDRVELLPADRKEKARKRLEERMKIRKNAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3T6</accession>
    <name>A0A9W8Y3T6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Autophagy-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ATG22_2</name>
        <name evidence="12" type="ORF">N0V83_008393</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Vacuolar effluxer which mediate the efflux of amino acids resulting from autophagic degradation. The release of autophagic amino acids allows the maintenance of protein synthesis and viability during nitrogen starvation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Vacuole membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATG22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3T6"/>
    <dbReference type="OrthoDB" id="192733at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032974">
        <property type="term" value="P:amino acid transmembrane export from vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17483">
        <property type="entry name" value="MFS_Atg22_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044738">
        <property type="entry name" value="Atg22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024671">
        <property type="entry name" value="Atg22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050495">
        <property type="entry name" value="ATG22/LtaA_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23519">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23519:SF1">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11700">
        <property type="entry name" value="ATG22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 10" id="KW-0072">Autophagy</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <keyword evidence="4 10" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="410"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="448"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="478"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="512"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="552"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="617"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="257"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="659"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="260"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="277"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4365772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="683" mass="75047" checksum="B0CEE886DB13F064" modified="2023-11-08" version="1">MPPHAPELPPYRPQLLRNVSTHSKASIPSISPSNEADDEQSLSEHTSDMESNAPQPHYDGEDTRLTSDKELRGFYMYGWAAEVFVVCGIGSFIPVTLEQLARENGVLLADRSTPCKASRPTVHPPQSFSTGPLEALFKPNPEKGQCVVNILGIEINTASFAMYTFSISVLIQALLIISMSGAADHGRFRKTFLLWFAFVGSIATMLFLPVVPKVYILGAVLAIIANTCFGASFVLLNSFLPLLVRFHPTVKFADSNENSYLDEGEEGEEEVEEEEENRERAGESREDQIAREAHYASQLSQRQTVFEEVVDATTALLPRDRSAADLTVPRAKSQSAPSPELALSTQISSYGIAIGYIAALLVQTIGLFVVIAFQSSNFGLRLVLFIIGAWWFIFTLPTARWLRPRPGPPLHIGAHTSNFGTVLAYFTYSWKSLGRTATHAGHLKDVLLFLAAWFLLSDSIATVSGTAVLFAKTTLGMSYAMLALINVIATVCGVLGAFFWSRLSLYLRLSPSQTILSCILLFEVIPIYGLLGYIPAIQRLGFLGLQQQWEMYPLGAVYGFVLGGLSSYCRALFGELIPPGYEAAFYALYAITDKGSSIFGPAIVGAITDAYGEIRPAFWFLAILIGLPLPIMMLVDVDRGRREGLALAKTLQDLSRARDAHSEDNLSQEIRDDLDDSYSYQRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3X5</accession>
    <name>A0A9W8Y3X5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">YCK2</name>
        <name evidence="14" type="ORF">N0V83_008909</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3X5"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14127">
        <property type="entry name" value="STKc_CK1_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000160">
        <property type="entry name" value="Casein kinase I 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="15"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="351"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="371"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="396"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4365290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="450" mass="50828" checksum="A4AD612C2BAD0382" modified="2023-11-08" version="1">MASGSTSSNVVGVHYRVGKKIGEGSFGVIFEGTNLLNQQQVAIKFEPRKSDAPQLRDEYRTYKILVGCPGIPNVYYFGQEGLHNILVIDLLGPSLEDLFDHCNRKFSIKTVVMVAKQMLSRVQTIHEKNLIYRDIKPDNFLIGRPGSKSANVIHVVDFGMAKQYRDPKTKQHIPYRERKSLSGTARYMSINTHLGREQSRRDDLEALGHVFMYFLRGGLPWQGLKAATNKQKYEKIGEKKQTTAIKDLCDSFPEEFNKYLSYVRNLGFEDTPDYDYLRELFTQALKSTGEIEDGEYDWMKLNNGKGWEVLKSHPSAVQAMHHSNVNQNSSMAVNLQGQQHRQNKTHLPIDHHRLNEPLPKPGATRAQPGRSPRDINNRDSQVKRKSMGELAPPEGASTQAQFQHSQPNLGSANRATPAASPNVQQPRRQEPEKPSFMQKLSNILCCGAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y492</accession>
    <name>A0A9W8Y492_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006849</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y492"/>
    <dbReference type="OrthoDB" id="63112at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030681">
        <property type="term" value="C:multimeric ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000171">
        <property type="term" value="F:ribonuclease MRP activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013893">
        <property type="entry name" value="RNase_P_Rpp40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15396">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15396:SF1">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08584">
        <property type="entry name" value="Ribonuc_P_40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4367268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="367" mass="42094" checksum="8ED372946E836494" modified="2023-11-08" version="1">MLDIHRTDSVPDTKIYFSHSLLPSYIDPQNVSTKKKPFSTFHAQHFTHTLHLTLPSETWDLVRTALQTNPLLSNPHYARVHMSLAELLDAEFLAYYIKQGGPIMMLSEGRPLIDPYLFSLYDGKLTLELDRPTYERCGLQGGTPVEDGGRKHQKQRWRVEYDLRSPSMKHGKKGFGRLEWAAQNVLNRSLSWLFCHVGGADGSAEALREGREVLNLHAPRVEKAEMRVERLTGVVVPKIATRNVKDEVYGEEEALGMLEYLHMLNLGSPRVYQRDAIDAHLSRYEVPDLGARGEERDLVQVCWRGFIAPRFARELFLLVRECGFKGRGEMRGKVRGYGCGWGWRRREVVQYECAGVWWEEGLECHAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4D1</accession>
    <name>A0A9W8Y4D1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Two component histidine kinase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_007473</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4D1"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF60">
        <property type="entry name" value="HISTIDINE KINASE G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="372"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="793"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="182"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="644"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="924"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="185"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="198"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="656"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="684"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="726"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="934"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="951"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1000"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="854"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4366943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1024" mass="113957" checksum="7AE99B5EDCB6EA8D" modified="2023-11-08" version="1">MENLDKLTAAGLPVFDMNQEHLDQKDWSETSLGPRKSWPSALQCLVNSCILPMPHCAAIFWGNDLSIIHNLAWGRACGNLDGQGAAAQAGYSSEALSTLRAVLRGRTVKVGKHQALPVQSSQSSSLKIPAARFFLTDIIDSRESQLLLSTILDEHGARVGVLAQLLENHTVERYMPIDGLDKLSRKNPTKKVKNSKSLKNDKMGDKVDDLVDEEKGHDSDKEQADSTQTLLFQKFAELLPNGLAILDKNAEAIFVNDGFFKLTTNKGQNEFRAWPESIHPDDYDQVMAAYRKAFEGQHELQIEFRCASEATNDKNEGAWRMFLLRPLGDDPEVGFICAVVDITEIKEAQLTQERAAREAQDRKEQQERFIDMVSHEIRNPLSAVLHLAEEVKEITKEISPDHADISTKLADILDAADTILLCVSHQNTLVDDILSFSKLDSMMLTLVPREVRPKWDFSKALKVFSSEFKAKSIKFHYAMDVSYDDQKIDHVIADINRMKQVLVNLITNAIKFTSKTNGEKKITVSMGASSERPTSYPPNVIFFNEGKEAFHTDATMTSDWGSGKPLYLMVAVKDTGIGITPEGQAKLFERFRQATPKTQEKYGGSGLGLFISRKLCQLHGGDIGVSAKEGEGSTFGFFFKVRQSDGTSEDGRPPFQSRSNSETSNASNRAETPGLKQPRPGYSRANSNLQSIKEGQERPQPKTLTSHSGVDADDVDDSLKNPPTEYMKETHPASARDDRFEETERVADSISTEKPELEKKLPSLNRGETQRQENTAEDTSRSQSGQRREDKQSLLLVEDNLINQKVLRRQLQSRGFEVFVANNGQEAVDAVADRGKTAHDDPNNRDHFDCILMDQEMPVMDGNAATREIRALQSEGKAGYSHILGVSANVRDAQTKGMREAGMDEVISKPFKVNDLVDKIQGMISKNSAGQTKMDAERGQEKKEPTEEETKEAQGKRDDVAKEYKKRQDDEQAHRMILAIRQSSAEGRRNDGGNRPVSSDGKRGEESNGRDGDGRGGARPRTQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4T6</accession>
    <name>A0A9W8Y4T6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008077</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4T6"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="287" mass="31043" checksum="E27C40540CEC0169" modified="2023-11-08" version="1">MLVGTEDAAEDTVLEARMLEDAALVDDAAGVEEDAALVDDEETTVEDAALEEEDAALEEEDTTVEDAALEEEEDTGVEDAALEEEEDTGVEDAALEEEEDTGVEDAALDEEEDATTLEEVAALLDEEAAPVDEEDEAARLDEDATTLDEDDEALADDEAAPVDEEETTVEDAALEDEETAAEDDAARVDELVADGVALLDTDVERATLEDAEDDRAALEEAVEDRTVLEEAEDDWAVLEEAEDEAATEEVRDTLEELTAEEPELDAERDALLETEVERDALEEAEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4V2</accession>
    <name>A0A9W8Y4V2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_006356</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4V2"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047146">
        <property type="entry name" value="Cyt_P450_E_CYP52_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287:SF19">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="465" mass="53051" checksum="712AE65295C5C59E" modified="2023-11-08" version="1">MAVRSILFVILVFILSVLALLYSSKFSRWLTRSLLTWKHGCKEPPRPKINLDEINRTSAQNCTSLDTTTQLFNEYGKTYKTKRGGTVFLRTCDPEVSKAVLSTCFEKFGLQPIRYEGGNSFFGNGMLVTDGAQWKASRMLIRPTFDVAHVANLDRLGPFVDRFMSLLPRDGSTIDLLPLLKRLTLDISSDFIFGRSMDALSSPESGKDFMNAFHAAQRDVVIPNPALRSKEHYEGCQDVWNYIDARIDEAYSRISGSENKPPSETKHVRIIDELVKVSQDRISLRFLILSVFMPAHDNVAVALSNAFFHLARNPESWTKLRTEIISKASERLTYDLLGSFKYLNWVLRESGGPDGRSSLLVEKGDLIEMSFRSQQRDKSFWGDDAEKFCPERWAIIRPTWEYIPFSGGPRICPAIKLVYAETEYIMVRILKEFSGLENKDKVLQWVEERRLTFQSRNGAKVALVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4Z8</accession>
    <name>A0A9W8Y4Z8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_007808</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4Z8"/>
    <dbReference type="OrthoDB" id="432528at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14733">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="464"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="651"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="666"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4366173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="682" mass="75420" checksum="7AA477AD01E3D506" modified="2023-11-08" version="1">MSASPARSRRSAVEGYKPKMISTIGAKPACLVNASVTYVGDDQIYAFGGFDQYTDEVYNHVLRLNLITRQWNLVDNYGDIPGVRMGHTACLWQGSKLLVFGGENEHRQHLADVIVFDLKTAHWTQPELHGPIPRGRARHSAVIYDDKLYISGGQTGHDSVLDDICYLDLKTWTWSRTWRFVPRYDHASWIWNGRIWIFGGINDDMEKSNEIWWLDLRGSPEFDHAMKYGTGDRQLISSRHRYAVPASGQLATGSTGYTANSSVQSYHGMSSNRSPYVAPGSISSLKFHSSPNLPSQAAGTHFHIFSSGCMLDFVTPAELSCETSLSSLELDTLRWQKLAEGQDLYNPNYRWHYCTTNPEGTHAWLLGSPIESAADGNHNGEYLSDVLAIDLRKYGILGNELASDSRSKMMPSSDANVSSHLTGIGADLSLTFDQPPESGSGADFIITADPDEDDYVDVAAPDPSASSTAVGPVSRPIHVHKLILHARWPHFARLWSAQMAEFHSKKMHIPEPYSAVRAFLFYLYTDSIAPNPQNGPSLSDVAGMLVMSNIYDMPRLRLLCVNRLGRELDIEHAAIIWERAATAGEDWLKQRAALFCMQNFGRLVRTAGFRNLSPASLVELCCEASPESRVVGGEDLELLGQITGRPFGVASNRKRSLGSAGVLTAGEEDGDEEVEDEGMDVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5G3</accession>
    <name>A0A9W8Y5G3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007101</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5G3"/>
    <dbReference type="OrthoDB" id="5430812at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494:SF1">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="121"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="593" mass="68378" checksum="1A3B8827DA83D67D" modified="2023-11-08" version="1">MAGPFRPASPAEDLPTYYHDLGDEAIQHCFRDFDHERNFDLFDRQTRNPRSSNFCVDFGEDDAYCAFDLDASSYSKLLSSPRPQQLHTRWINIWMPHNQKDLIRTLAGHFDFTPRLLGTMRSDPVPPRSVSLHKKKSSSTLRSRFSHKSNKSESIKARAREANQASLDLEESIGMSEIMQSTQLEVVRDLNHYQLVDDVWHWSTVDWGRRFVCIGYNSLHNVRTKQIDEHPEDADRSRDMPHGKRVWNWLLLCEDKTVVSVSEDPFPSTNGELTAQDLQMLYTTRRNLVNVFRQLTKAPTPLNDAALQQLPIRQRVGNSDEETAHRPTDAPGLLFYYLFEDWGATFNLISRRQHGYAAELDRLRKEMLVKADLTHVDQLHHIGRQLAVLKRVYQSYELIVERVLKKQEVTLASLKNSHIMSGDQSLASSIPGVGSPGPTMPEADSLLGVSLSSAARVRFERLKDRIVLYALSEIQECLDQKDSLVMMNFNLIAIKESFSVERLTWVTLLLAKITILFTPVTLMTGYFSIQFKNTEFEIKSYWRGSRNDFRMLRPQLLQHLDNNGLHRKIIADHQRVHSALLRGELEARYASWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5T2</accession>
    <name>A0A9W8Y5T2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_007397</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5T2"/>
    <dbReference type="OrthoDB" id="2898618at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052178">
        <property type="entry name" value="Sec_Metab_Biosynth_SDR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618">
        <property type="entry name" value="7-ALPHA-HYDROXYSTEROID DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618:SF18">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE_REDUCTASE FAMILY (AFU_ORTHOLOGUE AFUA_5G12480)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4366869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="294" mass="30782" checksum="EA4815EDA4B01831" modified="2023-11-08" version="1">MSASLTAGALFNVNGIVAVVTGGGSGIGLMIAKVLVINGAHRVYIVGRREDMLRKAAEDINPDIVIAVPGDVTSRDSILQIAGRVRSEVGYINLLIANAGIMGPKPLKPATGEAPPSIAEYRAHALKTPMTDFTETYAVNASGVYYTTLAFMELLATGNKKANMGPDWRSQVIATSSIGGFSRLPGASFAYNSSKAAVTHMMKMMATAFVPYKIRCNVLAPGIFPSDLASGIMNGLDPEKTGRLEQAIVPGERPGKEEDIAGAILYLASQAGAYLNGSVVVVDGGRLSTLVSTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y670</accession>
    <name>A0A9W8Y670_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial pyruvate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_006012</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the uptake of pyruvate into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y670"/>
    <dbReference type="OrthoDB" id="869189at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006850">
        <property type="term" value="P:pyruvate import into mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005336">
        <property type="entry name" value="MPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14154">
        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03650">
        <property type="entry name" value="MPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4368930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="159" mass="17555" checksum="BECC22204E88EC5E" modified="2023-11-08" version="1">MSFRPGSRIFNNFRPFFQNARRRVNTAAGAEPAGFAKFWNSPVGPKTVHFWAPVMKWGMVLAGASDFSRPAESLSFTQNFALMCTGAIWTRWCFVIRPKNVALAAVNALVFAVGATQVSRIYMYNKSLKSTEGQAKLEGKELEADLKATAEKAEKAVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y693</accession>
    <name>A0A9W8Y693_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Alpha-aminoadipate reductase</fullName>
            <ecNumber evidence="6">1.2.1.31</ecNumber>
            <ecNumber evidence="5">1.2.1.95</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">L-aminoadipate-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LYS2</name>
        <name evidence="19" type="ORF">N0V83_006207</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(S)-2-amino-6-oxohexanoate + AMP + diphosphate + NADP(+) = L-2-aminoadipate + ATP + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46936"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="1.2.1.95"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(S)-2-amino-6-oxohexanoate + NAD(+) + H2O = L-2-aminoadipate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12308"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
            <dbReference type="EC" id="1.2.1.31"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(S)-2-amino-6-oxohexanoate + NADP(+) + H2O = L-2-aminoadipate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12304"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
            <dbReference type="EC" id="1.2.1.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pantetheine 4'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:47942"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.31" evidence="6"/>
    <dbReference type="EC" id="1.2.1.95" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y693"/>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004043">
        <property type="term" value="F:L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05235">
        <property type="entry name" value="SDR_e1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000787">
        <property type="entry name" value="L-2-aminoadipate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010071">
        <property type="entry name" value="AA_adenyl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014397">
        <property type="entry name" value="Lys2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010080">
        <property type="entry name" value="Thioester_reductase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01733">
        <property type="entry name" value="AA-adenyl-dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03443">
        <property type="entry name" value="alpha_am_amid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01746">
        <property type="entry name" value="Thioester-redct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44845">
        <property type="entry name" value="CARRIER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44845:SF1">
        <property type="entry name" value="L-2-AMINOADIPATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001617">
        <property type="entry name" value="Alpha-AR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="11 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="18">
        <location>
            <begin position="636"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ4369123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1179" mass="130140" checksum="6FF39F300F44C0B4" modified="2023-11-08" version="1">MASLTKGEIPDPTADLHWSDFKGPIHEIFAANARKHPERPCVVETATSRIPERKFTYKQIFEATSVLAHHLVQSGIQRGEVVMIFAHRGVDLVVAIMAVLAAGATFSVLDPLYPPDRQCIYLEVSQPRALVVIDKATREAGPLSDQVRDYVKDNLQLRTEIPALELRDDGALVGGSVDGKDVLEAQQKLKAELPGVLVGPDSTPTLSFTSGSEGKPKGVKGRHFSLTHYFPWMAETFGLSENDKFTMLSGIAHDPIQRDIFTPLFVGAQLLVPSKEDIQHEKLAEWMRKYGATVTHLTPAMGQILVGGASAIFPTLHHSFFVGDLLIKRDCRRLQNLAPNVRIVNMYGTTETQRAVSYYELPSCTEAPDFLDTIGEVIPAGRGMSNVQLLVVDREDRNKICEPGQSGEIYVRAGGLAEEYLGLPEMTSAKFVKNWFVDNQKWVEEDKKTVESLGVAEPWRDFYTGPRDRLYRSGDLGHYGPDGNVHCTGRVDSQVKIRGFRIELGEIDSHLSAHPLVRENVTLLKRDAYEEPTLVSYIVPEMKRWYDWLEERGAKDHDSVEDTSLVTLLKRFKYLRDDVREHLKKKLPAYAVPSVIVPLIRFPLNPNGKIDRPALPFPEPAELAAAGARRPSQLGAALTPTEKTMADIWAGLLSDRGVTADSISGSDSFFDLGGHSIIAQQLLFKIRQEWKDIDVPMTTIFQYPTLRGFSANIDQAMDPIGLRLDTAEAIEDDPEDEAYSADARDLATQLRDFDAREPLKVGEEVHTFLTGATGFLGAYILRDILSRPGKVTVLVRAQNIDTAFGRVRQTCQAYGIWEDSWESRLEPLVGDLEKPNFGLAPATWNKLADSVDVVIHNGALVHWVLPYSRLRGANVVSTMTALAMCAIGKPKNFGLVSSTSVLDTDYFVKLSEKSLAGGGTGVPEADDLEGARKGLGTGYGQSKWVAEYLTRQAGKRGLSGCVIRPGYVLGDPEYGTTNTDDFLVRILKGCIQLKSRPDISNTINMVPVTHVARVVVASSFNPPISPLGVAQITSHPRITFNEFLGSLENFGYNVPQVSYAEWKKNMETYVADRSGTKEESALLPLYHFVTGDLPADTKAPELDDRNAAEALRKDQAWTGQDWSAGGAVTEDTVGVYISYLIELGFMPKPEAKGFKELLVSKMTDAMREGMKLVGGRRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7E0</accession>
    <name>A0A9W8Y7E0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005444</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7E0"/>
    <dbReference type="OrthoDB" id="3796950at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="402" mass="45199" checksum="D0BEFE473E1CD785" modified="2023-11-08" version="1">MREETAEGCSSLDEGTESGDQAVGFELCRVRSRQPSPDHKDGFLDIALTSNPDSIVLRRVRYAMLAAFCSAAYIQRYVARRPDGSEILMLPAGRCEPRGLKTAIRWMELAYNGESECVLPRQFHGSDLDACYAERAFAVLGLHRKAKHIRLNVNGIFFTHGITVEFLQAVLETLPRRSHWVKKVLGYIRRCHANYSKNGQRNGNLLVCNVCGVSILESSSVHSEPEGLVEFVEWAMRDETFRKAVVGTYKTSRDAHLHVDIAADSFPATVSRTETWIMNVEDHESHPFGQFPDTSDLIGDVTKRIPTYRRGSGSECIYGLEWSERDGGCYIETYSSRREASSTFCEMQATEMSREWLASRGYRSKSADFGSIRSAPPGLREGSDQPDRQEKMATPKIRALSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7F0</accession>
    <name>A0A9W8Y7F0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_005460</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7F0"/>
    <dbReference type="OrthoDB" id="3800281at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056125">
        <property type="entry name" value="DUF7708"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054471">
        <property type="entry name" value="GPIID_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF14">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24809">
        <property type="entry name" value="DUF7708"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22939">
        <property type="entry name" value="WHD_GPIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7708" evidence="3">
        <location>
            <begin position="75"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase winged helix" evidence="2">
        <location>
            <begin position="320"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1013"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1026"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1040"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1070"/>
            <end position="1084"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4369697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1237" mass="138811" checksum="26FDA6FC65B91036" modified="2023-11-08" version="1">MVGAANMAGPLTRREDKVRTRLNDALVRYHEALLGCVDTSTAPLASAEDVLDVLSDVAAKQQQRQSLKWLQSGNAVVSCVMQYDAVFATYANIYPAVTASLWGTLKLLMEVASKFVDAFARLMDMLKRLAENLPRIEIYEELFPSNEQVQVVVALLYGDVVEFCVGSYKFFSRKALVIDAVDESDDPMGVLSPLFDLCTATQGSIVHLFIFSRSSLQIPDKVSGVLTREITTKDTRQDLETYVDAALNRKPLSNLVDEPDLSQRIFRTLTSPSHGLFLWVKLMIDTLERQLLLRDIIEALDNLPKGLDEVYGRLLSPLLLQSDKNREAARHILAWLCVAPSALTLSQLGKAIAVRQGDNTEDQGARVLDLSGMLRKLFGPLIAISEDSQTVTFVHITIKEFLMQPLSAWSQSSDLAPLKAFHIDPEWANAYVSSTCITYMSYDCFRPTQHEKITSAGELNKSVDFINGNELYEYCMRYWADHLTQSGIPSTDLLSLVHRFVNSSGVEVWYENVSQKLSKDQWGQKTNDLDTVEITSMQFKLNRWLQQSKTWATKDYLASDIIEQIHSRILKQREHRYGPEHLKTLQSKNTLAQLYFAQSRAKDALPLYLAVCEGFQRILGAEDIETCRALVGLGDTYSSMSQHDTAEKHIRIALLALQRTLSPDDPAVLKACERLGLVLSRIKQGPTNPHAEELRKLRFRVYDGRMRTLGPEHLETLKAMLDWGQCLRRFGDTQEAERMISTSLEIRKRILGPKHPAVLDHKKNYALLLSGTGRKVEAVEILRECLSVQEELFGIYHNDYLRTITNLAGTLRQLGEGEKGPPGALDEAESLARTVIEIRTKLHGAQSSDCAESILAHANILMALGRLFHAREQAKNALQIFTSCPLDTIRTSGVQRTANKLVDVLRKEKRLREADNVSKAYLKQPAGPYPPQYPGSRISLGFFQAFLVVLVALLILVDLDALEVQLSKTSSNSSKARKNIRKLIDDSASESDMDTDFEGSEEEIDDEYNELANLSADNVAYNTADPVDRRESYDSRYEPGNEDYERDDFVVDDDDDDDESGDDSERTAEVEDDSQDEDNTDEDYTPPPRRIIRKEIIILEQQEIKENTMVRKASLRSTRSLSSASGGLFVTDEDEPPVSVQGYPGSPPSDLIKYQPYLETPVTSGAPEDIVEELTDAIALFSRRCNRKRTPLTLRLCVEALENEEIVEALQDAERLGMGEGLRLSDGTEVWTIGPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y847</accession>
    <name>A0A9W8Y847_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inhibitor I9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005512</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y847"/>
    <dbReference type="OrthoDB" id="3888684at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052471">
        <property type="entry name" value="PBI_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28288:SF1">
        <property type="entry name" value="INHIBITOR I9 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28288">
        <property type="entry name" value="PROTEASE B INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9025" checksum="0B5D9F534CBE30CA" modified="2023-11-08" version="1">MAVAPQRSVIISWPNETPDQIVEDAKEAIRKAKGVITHEYNIIKGFAAQAPASALEFVSTMSDVYKCEIEEDGVVTTQDKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y963</accession>
    <name>A0A9W8Y963_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">High copy suppressor of abf2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">YHM1</name>
        <name evidence="12" type="ORF">N0V83_005590</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y963"/>
    <dbReference type="OrthoDB" id="409947at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001409">
        <property type="term" value="F:guanine nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000010">
        <property type="entry name" value="Probable YHM1 (Mitochondrial carrier)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053042">
        <property type="entry name" value="Mito_GTP/GDP_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46974">
        <property type="entry name" value="MITOCHONDRIAL GTP/GDP CARRIER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46974:SF1">
        <property type="entry name" value="MITOCHONDRIAL GTP_GDP CARRIER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="231"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4369826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="318" mass="34389" checksum="77F08CAE77D1AB5C" modified="2023-11-08" version="1">MTPTPSEAVAKVAQVTTGTNSSAKVYKRESNTARVLGAGCAGIAELMVFHPVDTTAKRLMSNQGKITSASHLNKVIFRDAAEASIPSRFLSMFPGLGYAAAYKILQRVYKYGGQPAVRDYLAHNHGDSFDRTFGRGNGKAIMHATAGSLIGIGEIVLLPLDVLKIKRQTNPEAFRGRGLFRIIADEGFGLYRGWGWTAARNAPGSFALFGGSAAAKEYLYKLSDYNSASWGQNFVASIAGASASLVVSAPLDVIKTRIQNKNFENPESGFRIVSNMMKHEGFTSFFKGLTPKLLMTGPKLVFSFWLAQTLIPAFGKIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9X2</accession>
    <name>A0A9W8Y9X2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_004699</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9X2"/>
    <dbReference type="OrthoDB" id="70376at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013907">
        <property type="entry name" value="Sds3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21964">
        <property type="entry name" value="BREAST CANCER METASTASIS-SUPPRESSOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08598">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01401">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="204"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="326"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="531"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="330"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="351"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4371482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="557" mass="59951" checksum="ECDF4F83836A78A5" modified="2023-11-08" version="1">MVRNGSPSPADNFPLGHSATSPQAQPTKRDKRRNMLAEKLADMMASFSDNRDSHYRAQLAALQADINLIMKADPYANKPLEDAGAEASELVSQIMGNNVPSAPSAGTDYVAQCGKHYSRFVDAVNDAMEERDYNLTMLWNKHENSKNEIENSHFYKVQIAEEEHKLLAATIRERLIASIQTRTGRLKREKEQLDLSDSNAMLLHPNQFSIGNPASPGGPQASRKTRRTGHKFGDAEDIAAANENKRKRKLFEDNDNDSPGPSGRNIEMGIGSPFREAKARTINAQFESSAYSLERLFTEKELNMAMNQATTAASHFFAKMKHADAPHEATTNGHGTTNGNTNGEHASDNGDTVDPDQDSDDIPGASDMVRQVSSNPHATRGATRSALNPTAAIANGQMPFIYQPPFVLDAKVFQKLNAAAPTPPALSAQDVEQDLKLMLRDARPDDELNEKLLAAACNPVKARDYQVQPPGSSAPINEITTHIRNTAPLLEVGGFGGVPMSAQSSMAGWSDGGGNTPLRYGERESLAAALGGGHGGGVAMSRTASGSGRGRGRGRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA43</accession>
    <name>A0A9W8YA43_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_005637</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA43"/>
    <dbReference type="OrthoDB" id="4676at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016813">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03884">
        <property type="entry name" value="M20_bAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010158">
        <property type="entry name" value="Amidase_Cbmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01879">
        <property type="entry name" value="hydantase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494">
        <property type="entry name" value="ALLANTOATE DEIMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494:SF20">
        <property type="entry name" value="PEPTIDASE M20 DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001235">
        <property type="entry name" value="Amidase_carbamoylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="3">
        <location>
            <begin position="218"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4369873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="427" mass="46574" checksum="CB65AE0F57CC407C" modified="2023-11-08" version="1">MASTSLKINGDRLNGTLQETCSQWGALSASTGMCRLALSHEDKEVRDWLVTECKSLGCEVKVDQMGNIFAIRPGTAKDRKPIAMGSHLDTQPAGGRYDGILGVQAALEVLRSLHYNKVETYCPVALVDWTNEEGARFPGAMMASGVWSTKSSTPLDACWDIVDKEGIRLKEALGEIGYLGETPCDYRENGFQCHFELHIEQGPLLEKERKSVGIVTSVQGMKWFAVRVEGEEGHSGTTPMSGRSDALVTTSRLITAVRDTALKSELGVATVGVIHSDTSSQATIPAGVDFIIDIRCSTDEMVDQLATAIIKAFDNIIEQEGNRTSYRIARSWGLPESKFHPHCIDAVRTSAVKQVGEEQVMEMKSRAGHDSAWTSRVCPTSMIFVPSKDGVSHNPNEYTSPDHCALGAQVLLDAVLLYDENVKSGKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA99</accession>
    <name>A0A9W8YA99_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003997</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA99"/>
    <dbReference type="OrthoDB" id="6365728at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090141">
        <property type="term" value="P:positive regulation of mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045909">
        <property type="entry name" value="MID49/MID51"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16451:SF7">
        <property type="entry name" value="MAB-21-LIKE HHH_H2TH-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16451">
        <property type="entry name" value="MITOCHONDRIAL DYNAMICS PROTEINS 49/51 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="144"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="430"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1019"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="869"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1092"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="68"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="308"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="450"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="475"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="524"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="602"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="735"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1020"/>
            <end position="1044"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1197" mass="134813" checksum="7E5DE7AFFE6D7DB7" modified="2023-11-08" version="1">MRTITVTIMAKKKNKKNQKQVELASPPEPPTEEPVPAVPDDPSLTAEAPATASTKDRPITDTPATDATSRDEIPSLARKTDDATIVERVVEGPTSAPSQGAEDLRTLPIEGEQPSTSQDSNLAEVVPVLKQEVVVPQEEDIAQSIKKSEVEQQEDQASEQQVDTAEPTEAPEPSKGPIETTETEAQSEPLQQSDPLTQQESENVLIAPKEEESMPAAEATPQIEPIEEAAAEPTNVPEAPEAETNPAAIESLPTDLEEPGQKDSVSIDVAEVPDGSDSLPPPAAPEAPSVQPPSLQEKSTVESASVTPKKETAKQKRTRENKEKKQREEQERLEQEQRLETEKLEQQKLELEQREQEAEADKQRTEQERLEQEAKDAADAQKAAAAKEEEHRQELERLAKLAQEKLAQEKLAQEKLAQEKLAQEKLAQEKLEQERIEQEAQDAADAVEQAQKEAEANEKSRLEQEAIEAAQVAEAAEKAQKEAEIEAEKQREEQERQEQERQREEQEKQEQARLEQEAAEAAEAAEKAQKDAEAEEEQRKKEADKLEEERKENERLEKERTEHERLEQEAKDAAQAAERAQEEAEVAEAARVAAEHALREPTPIPEPTPIPEPPAEPARAPSPPSRHSPHSLMTEEPKQRVPETGPIHAPRPQAVTQIIEDNRPPAPSPPVGRMRSRHIPAFDSEDDSDSALVQTRRPFRGGESRDTSRGSTYPPPPTHTRGVFASRVPAESYTNPPPPPPPGYHPRYYAPTAPPHYQNHGYGMSQQGYPTPNPYGPSSHSSSSPYQEPWNQYPPGYPPYGSPPQRHDSSGSRDYPVHAIESGSAIENDSGDVFSRIAQAIPDLHVLLARYKETHGQLSVREELLRRASVEQEEKLSAKDDEIEDLKEKLRNLENKHSTEASRLRFQIGNLEEQVKELREQIAETEKYKKEAEDTKSALDAAMKSWEAKYKALEEAHVVLERTAAEEKAKVWRDFDEWKSTATTKHDAEKIALAIQFDKKLKEADVLAENQRQEAATAFVKEKDELRSEHQRQQREREASFDRVRNELETKLGSAQKDREEALKHERESREFWVAERETLLKAHQEDHDSLQKGWNEQRDLLEAQYKKTKDESDKAWIDLHAEASRRADEEKAKVDQLTKEREELIKKYDELKAESQKEKEVIKSVATNLESEKARLEKLMDCYGDIAEIKSKGDTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAG2</accession>
    <name>A0A9W8YAG2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable endonuclease LCL3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Probable endonuclease lcl3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">lcl3</name>
        <name evidence="17" type="ORF">N0V83_004087</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LCL3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAG2"/>
    <dbReference type="OrthoDB" id="430293at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000029">
        <property type="entry name" value="Probable endonuclease lcl3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 17" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="TNase-like" evidence="16">
        <location>
            <begin position="77"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="243"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4372313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="271" mass="31054" checksum="5EB98E53AAB84197" modified="2023-11-08" version="1">MRWPWSGNDDDQKSSGRTAPAAPLKSNGWASTFTEPQTLIPSIALTITTVVGVRLYKTYLRRIPTVNHIKPNYFRRRSLFGQVTSVGDADNFRLFHTPGGRLAGWGWLPWKKVPTKREELSNQTLHIRLAGVDAPELAHWGREAQPYSKEAFEWLTSLIHNRRVRAHIYRRDQYDRVVAQVYVRRWLLKKDVGLEMLKIGLATVYEAKTGAEFGAFEEKYRAAERNARERKIGMWTKPSLIQRLGGAGTKAPESPREYKNRHTAAEKKKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB98</accession>
    <name>A0A9W8YB98_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_005104</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB98"/>
    <dbReference type="OrthoDB" id="1684102at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000067">
        <property type="entry name" value="Transmembrane domain transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF666">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="430"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="469"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="495"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="535"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="568"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="615"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="657"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="680"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="713"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="351"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="309"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="339"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4370583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="752" mass="82471" checksum="17E511292FD5DE7B" modified="2023-11-08" version="1">MAGDRLASSRPLAVPSSSHDSGRNSPSESQSVTRASSIARLGSPVPSVPTGSSVRQIPTPAQLEDSPSVQTPLAGRTEQSSLPGPGTSALSSALKESLGRSPPRFGTPPIQPQSPAQKAPSSRGPPSNYGSFDGRGRSPVPYEDPEIVRRHLVGQYASPSRSNRGFLGDEDTASQGRRAQDEEDQEFSSLQLQGGDITRQIYRWSEQQQAEEQGKLQRSKSFHGARRRPEEEVLDIDSIRQPGGFRRNYLRRNAGSPSPAPGPSGYGTVRQARQQPQVFTSNFLEFLTLYGHFAGESLEEDDEVLGPDEYFSSDALDSGEHTEDDDREYGESSALLTPGKRRKRKPKKTGNGSPTGAAMLLLKSFVGTGVLFLPRAFLNGGMLFSNLVLLAVAGLSYTCFVLLVSTRLVVEHSFGDMGFHLYGNWMRNMINFSLVISQIGFSSAYIVFVSENLQAFVLAVSDCRTYLDIKVMILMQMVIFLPLSLYRNINHIQKLALVADLFILLGLVYLYYYDLFTIVHQGGVSDIAAFNPRDWTLFIGTAIFTFEGIGLIIPIQTSMKDPKKFPKVLGGVMIIITVIFLSAGALSYAAFGSKTKTVVLLNMPQDNKFVNGVQFIYSLAILLSTPLQIYPAIEITSQQLFSRTGKYNPYVKWKKNFFRFFMVLVCATLAWAGAGDLDKFVSLVGSFACIPLVFIYPPMLHYRAVARTTFHRVLDVLLGILGVVGMGYTTALTINSWINGGAPKAPGYCDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBS3</accession>
    <name>A0A9W8YBS3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dynactin subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_004565</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynactin subunits 5/6 family. Dynactin subunit 6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBS3"/>
    <dbReference type="OrthoDB" id="2355at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027777">
        <property type="entry name" value="DCTN6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13072">
        <property type="entry name" value="DYNACTIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13072:SF0">
        <property type="entry name" value="DYNACTIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4371348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="228" mass="23962" checksum="151E47BDDD8AE649" modified="2023-11-08" version="1">MSAQPAPARPAPDRRSSTVPKRTSLLPKPSSIILDASVLIAQHAQLTGTQPITIGSNSVLHPHSRISSALAPVVLGEGCVVYERAKIGVGMGAELDAESRRSSVASSRNSASTASIRGEGTVLGNNVVVESNAIVEAAEIGDGSVIQVGAVLGRGCTIGKYCTISAASIIPQNARLPDYTVVFSGCEQRIDKTLQLRPEILESKMAIHSKQLEMFKKLIPNNVAKWTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBV4</accession>
    <name>A0A9W8YBV4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tudor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_004610</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBV4"/>
    <dbReference type="OrthoDB" id="79171at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tudor" evidence="3">
        <location>
            <begin position="116"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="167"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="281"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4371393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="324" mass="36102" checksum="E8138CB7534C1AB5" modified="2023-11-08" version="1">MADHVKQAKSKIGEIKRRIQDAETQRAEWASTIPGIDTELASLKPGDGEEWKQMKEEAERSVATFEANIAKYKEQLAALEAELPQAPEPVVPKFDPEKHPLLRKTVEKQEPEKAVVFSTGDMCEAQWTDKSWYKAKIQSILGSVSAPKYLVRFIEYDDTMTVDRSAVRPLPNKRKREVEPAPQPAAPVTSTPHVISGPASVNPHAQAANSNATNDVAEPKKASRVPNKGILKKRANAWNDFQAKNKKKGQLPKESMFRTSTEFGSRGESYHDCTLDDSVDHNTVGFTGSGKGMTQTNKRTRYDHKEDARNDAATKGDDQNESRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC62</accession>
    <name>A0A9W8YC62_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">SWIRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_004088</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC62"/>
    <dbReference type="OrthoDB" id="9982100at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033193">
        <property type="term" value="C:Lsd1/2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031934">
        <property type="term" value="C:mating-type region heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005721">
        <property type="term" value="C:pericentric heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140720">
        <property type="term" value="C:subtelomeric heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140683">
        <property type="term" value="F:histone H3K9me/H3K9me2 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140718">
        <property type="term" value="P:facultative heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033696">
        <property type="term" value="P:heterochromatin boundary formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071515">
        <property type="term" value="P:mating-type locus imprinting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031508">
        <property type="term" value="P:pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000064">
        <property type="entry name" value="Lysine-specific histone demethylase 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000067">
        <property type="entry name" value="Lysine-specific histone demethylase Aof2, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF386">
        <property type="entry name" value="LYSINE-SPECIFIC HISTONE DEMETHYLASE 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SWIRM" evidence="10">
        <location>
            <begin position="227"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="HMG box" evidence="9">
        <location>
            <begin position="996"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="6">
        <location>
            <begin position="996"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="61"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="166"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="941"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1058"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="114"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="963"/>
            <end position="972"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4372314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1125" mass="123445" checksum="5589C62FD33DC7DB" modified="2023-11-08" version="1">MFSTFASTTESSALDDGSSLFDQSHDDLLSLPLSHDSIHLKGGYSPITGLSNMGYGKETASSSVHYVDSHDTSEPSNGHLSGRDEPGGSTPGAFIHGYGPNYTPSPLQYPVTPHPNSRNGTSNLKSKSTFSDGGITRTAFGDTIAVQSKPQLSTKDSRGDIAFMARTPQSLGRDSQPSSTPELPQSSDDRGKHRRTILTAANGKSSELKANSSIPSSLSWPEFGRQCILAAENSRLNPFALHPAEYRLLRDHVTHAQVTIYLNIRNAILRLWHRNPLVYVSYEEAAGCARDKRYFGLARVAYLWLLRNGYINFGCVDVPNTAGSVPRNKAKGASTRTIIVVGAGMSGLGCARHLEGLFAQLAGQLADMGERPPKIIVLEARPRVGGRVYSHPFLNQSGSTLPAGHRCTAEMGAQIVTGFEHGNPMNAIIRGQLGIPYHGLRDNTILYDYDGTVVETSRDILVEKLYNDVLERASVYRNKPVPQRTVEGDRNLMLFGREPTDTGGLTVAELEHSDAPLSANANSTASIKEEKPTTGVEKLAGRAYQLTAGFNPDITAAEAVQSLGWKLKPDVSTSQSINLDTIAKAADYPTLGHTMDEGLRQYQTLVDLKPRDMRLLSWHHANLEYANAVSVNQLSLSGWDQDIGNEFEGEHTEIIGGYQQVPRGLWQCPSKLDVRFNTPIKSVHYNTSQYIEGGRAGPAVRVECNNGEVYEADQIILTTPLGVLKSGSITFQPPLPDWKQDVIDRMGFGLLNKIILVYEKAFWEPDRDMFGLLNEAEREASMRPEDYSAKRGRFYLFWNCIKTSGKPVLVALMAGDAAHYAEATSNDQLIKEVTDRLDSMFAPNPVPLPSETIVTRWRRDPFACGSYSYVGPKTQAGDYDVMARPHGPLHFAGEATCGTHPATVHGAYLSGLRVAAEVAETLLGPIRVPTPLVEKRVVKLEHTSTTSSAEPKRKFAPAVSPPKETKPARVQRDEDYEAAIIGSILEQIGERPIKPGRTGVNPFLLYTKDFWYICKKEGDDARKAATGNPEAKASKQEIRNAIGLRWRTASQEVKQPYLDQASDARNDATANAADFKERVATWDKEAARIRQEYIQQHPPEGGNEDLILNSRTAIELGAGKRLRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCA2</accession>
    <name>A0A9W8YCA2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">High affinity glucose transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">HGT1_2</name>
        <name evidence="11" type="ORF">N0V83_003697</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCA2"/>
    <dbReference type="OrthoDB" id="4142200at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000026">
        <property type="entry name" value="MFS quinate transporter QutD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF7">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="314"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="453"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="10"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="508"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4373402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="532" mass="58732" checksum="93E37BA40858FC65" modified="2023-11-08" version="1">MYQIGNIYGITAIAVIGGGLFGFDISSMSAILPTQQYRCYFNQGPEGPPFTDSTDCSGPNANVQGGITAAMPGGSFLGALVSGYLTDKLGRKRAIQIGCMIWIIGSIISCASQNIGMLIVGRFINGLSVGICSAQVPVYVSELAPPSKRGRVVGSQQWAITWGILIMYYVSYGCTFLDGPTAFRVPWALQMIPAIILAIGLLFLPESPRWLARHDRWEETQAVLTLVHGKGDPHSPFVKLEMDEIKQMIEFERQNADVSVTELFRPRMLNRLHIGIFTQVWSQLTGMNVMMYYITYVFGMAGLSGNANLISSSIQYVINVFMTIPALLFMDRWGRRPMFVIGAVFMAIWMFTNAGLMASYGHPAPPGGLNNIAEQSWEITGAPSKAVIACTYLFVASYAPTWGPASWVYPPEVFPLRVRGKAVALTTSSNWIFNFALSYFVPPAFVSIKWKVYIVFGVFCTAMAIHTFFAFPETAGKTLEDVEEMFLAGVPAWKTKVDYSNTRRAEKGVLDPEKAAHFEQSPERRENAEMKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD79</accession>
    <name>A0A9W8YD79_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Flavin reductase like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_003437</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the flavoredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD79"/>
    <dbReference type="OrthoDB" id="10250990at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33798:SF5">
        <property type="entry name" value="FLAVIN REDUCTASE LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33798">
        <property type="entry name" value="FLAVOPROTEIN OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin reductase like" evidence="6">
        <location>
            <begin position="88"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4373146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="297" mass="32540" checksum="3D90F001F21E789B" modified="2023-11-08" version="1">MASGKLDAEKEIKRNPHGDFKAVERSRPPFDTSTSGSFHFTQTPQPDWKPGQGANTPSCLTKSHRQIDPYGDGRAIAHNYKLLISAIVPRPVGFTSTISADGTSTNLAPFSYFNMVNHDPPIFVLGFAGGMDRAKDTLKNLLDTKEAVINIITEEYVEAANFTSINAPEGVSEWAFSGLTAERSTFVRPDRVKEAVFSIEAKLVETREWTSKNPATPGKKTGVTAFLEGVNFWVREDAVDEQGVLIDPAVMKPISRLGGITYGRTTQAFELTRPDYDEVTKDPEVAKLAKAKGDSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDD1</accession>
    <name>A0A9W8YDD1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDD1"/>
    <dbReference type="OrthoDB" id="2386090at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4374309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="234" mass="27051" checksum="8749667C191C375D" modified="2023-11-08" version="1">MALLQFGTTLVFGVPPLWNNENQPNPDIRKFQAVLVGVLGTVPTLTLAYVTAPFAHQVFLQIPEYARRSRQDLMHFARTLRTNPKGTANTKLEFVTLRIFPFRKHTTAFLHELRALPPMKMRLANIELPKTEAWAQRQREKGIFKRMAEVIQEPRYKFYVKEGRLYTMKTGVPGVWEEVARRIQDQTVAEKDETGYSGLGARKPVVLRSVGQMKLGKEMDKQRLKRHTSRPVHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDE8</accession>
    <name>A0A9W8YDE8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">6-phosphogluconolactonase</fullName>
            <shortName evidence="6">6PGL</shortName>
            <ecNumber evidence="4 6">3.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SOL1</name>
        <name evidence="8" type="ORF">N0V83_002822</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12556"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="3.1.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.31" evidence="4 6"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDE8"/>
    <dbReference type="OrthoDB" id="432544at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01400">
        <property type="entry name" value="6PGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000005">
        <property type="entry name" value="6-phosphogluconolactonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005900">
        <property type="entry name" value="6-phosphogluconolactonase_DevB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039104">
        <property type="entry name" value="6PGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01198">
        <property type="entry name" value="pgl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054:SF0">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="7">
        <location>
            <begin position="12"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4374083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="262" mass="28867" checksum="DA1206FBED74A687" modified="2023-11-08" version="1">MGKPPNLYAFQSADELAPSLRTYVLDAQNSALERHHIFRVAVSGGSLPKTLAQALLQETNGDGKVQFDKWEIFYADERAVPLDHEDSNHRLVKEELLNKIPAELGTPKVYPIDTKYLDDVQELADQYEKTLVSVFAARDSVKLPLFDLLLLGCGPDGHTCSLFPGSELLRETEAWVLPISDSPKPPPKRITISLPVAQHGLKIGFVATGGGKKDVMRQIFDTDEGQQLPCGLVNLKGAERVSWFVDYAAIEGVSFPARRGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDG4</accession>
    <name>A0A9W8YDG4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDG4"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938:SF35">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 4, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938">
        <property type="entry name" value="RESPIRATORY COMPLEX I CHAPERONE (CIA84), PUTATIVE (AFU_ORTHOLOGUE AFUA_2G06020)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="604" mass="68672" checksum="AEB2C0B2E63A1A80" modified="2023-11-08" version="1">MFLSGKDEQALKEWEQDHSLDCARHDYEPEHLEAGARLHALAGNADRAREVMEKLYALYPDWDPSVMMTVFRAHTSSASEQHHKLAKDIYKWMKEKRGNEVSIDDYDSWLVGFLEAQHLHYAKLVFRDMIKNERLITTGSAEHIEEVLKRLHMLYRLGDDISKMTSIALDLLSVLPQAYHLHVFGDWMKSAVVHKAPGAAAQILDMMFQRGSKPQTFHFNMLLEALLRTKEDPNILKAENIGWRMIEEASKPHKEAMRSDSTTEIISKRHKRKKRPATTDAARKVPKADVATLALIMHHHAQNLQWEHVDYLARQLKQTSIEPNTMIMNVLIDNKCRQGAYVEAFAIYKALTSPIEGNSTVEGDSIIEGNSTSASAEDIFPDGATIRCLWKTLRLALSSKSFRDDPNLPSPRDLLKETVSWWTLSRSRYDASRFLQGLAGADHGAITALMLHCFSFTSDLPGSLIVLHVLRHKFDIFPTDKVAEILRRQVAWVDLTTETKAVRIHFAQQSRNGSGQREREKVARVYEILRERRMGKMGLGGKGQDRMGVEEMGDVELNLLSEFVRVVLKRRHPPDVVEAMLDDARKSVGLPDLPTGDLDAFEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDS0</accession>
    <name>A0A9W8YDS0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">40S ribosomal protein S4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RPS4</name>
        <name evidence="9" type="ORF">N0V83_002982</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eS4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDS0"/>
    <dbReference type="OrthoDB" id="1109245at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06087">
        <property type="entry name" value="KOW_RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000005">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.740:FF:000001">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00485">
        <property type="entry name" value="Ribosomal_eS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000876">
        <property type="entry name" value="Ribosomal_eS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032277">
        <property type="entry name" value="Ribosomal_eS4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013845">
        <property type="entry name" value="Ribosomal_eS4_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038237">
        <property type="entry name" value="Ribosomal_eS4_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041982">
        <property type="entry name" value="Ribosomal_eS4_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013843">
        <property type="entry name" value="Ribosomal_eS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018199">
        <property type="entry name" value="Ribosomal_eS4_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003312">
        <property type="entry name" value="PRK04313.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16121">
        <property type="entry name" value="40S_S4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00900">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08071">
        <property type="entry name" value="RS4NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002116">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00528">
        <property type="entry name" value="RIBOSOMAL_S4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RNA-binding S4" evidence="8">
        <location>
            <begin position="43"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4374241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="263" mass="29499" checksum="9B827F90D99C2D80" modified="2023-11-08" version="1">MGVRGLRKHQKRLSAPSHWLLDKLSGAYAPKASPGPHKLRDSLPLIIFLRNRLKYALNAREVNAILMQRLVKVDGKVRTDSTFPSGLMDVISIEKTGENFRLIYDTKGRFTVHRITDEEAQYKLGKVKRVQLGKGGIPYLVTHDARTIRYPDPSIKVNDTVKIDLSTGKISDFIAFDTGVIVMVTGGRNMGRVGVISHRERHDGGFNIVHLKDAVDNEFTTRETNVIVIGKEKPWISLPKGKGVKLSIAEERDRRRAYAIAGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YED8</accession>
    <name>A0A9W8YED8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003622</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YED8"/>
    <dbReference type="OrthoDB" id="3712212at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4373328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="186" mass="21366" checksum="A6790CA4892A4A00" modified="2023-11-08" version="1">MAYCGTNCVEPFGNAVDSAFICNECIQAEATKIWEERKAQHNASYPHIDQMTKEQYDQYYEERRQLEAEFAHDHKIYEMQLREKMQPSNICSAIEASKEEMDFAAEIDSLSLSMMASNASITDHQPQTRGRMNLPSDASEQLHWDLNTLALERGSCGIEYTATQPNNGIATMCRLDEDELWRKPRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFD4</accession>
    <name>A0A9W8YFD4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFD4"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="772" mass="85149" checksum="F12AD4EC077F4D89" modified="2023-11-08" version="1">MPSSDANSLQALKYPPVRVNHSLSTLDLDTLMMRSTTRRLPEEAGSSLDDSTYELLSESFETSDDEAHTESIASTDGPTPDDTSDFSDDDSVYETGQRDMNSSMHSSHVEETEQYPDVHSMVSGEDSTLTEVPPHMGGSESLRNIDLEEQPTEGSDVISASKVIKSVPNETSEMPQVLNRYGCSQVRLVVRAALSSRPIPTPESYRILYIGMPEKWMEDGITSKVGATLAAAPSVSRSVMVRDGQVEPYGPVIQIYRCAELHTFAEYEKQSHVLIILDDGTQLKFGPGLASHTEDQPDLVVFCHPTVTSPVTDVQEFPSAGEIFRRENIPCINLSLALARPYGDGANAYDPKSLRVCVEGRDDSGTDYELKEALPLDYYSFSELDPVQLNRHLALISPHLFPEADTNTQKTRRSLIGDTCGVVTKAVRSQWPFIKMLLPVVVFFALIPAFLQGAAYAPMLFHNRSNGEVNSSISVQYANMTSSVASTVLTSQTGHPVSIPSVRSTAKDLTIVPPQAMPPKVESEKKDDKINGFDIHTTGDHQFVLSPSKNFAKIRKTPQLQIQISRECAAVPARYNRTITGEYVVDLEQAYPISTFNVSIMTLSKPLLRQSFEITLGQNKSTLAEQFAELLETDWKDYIKRHNYFENASSTVAQQIRARYLGLETAVLRRTSDIGGSSEEALDRIRIAKEAIQRNVAIGAELLSRVPGATWIGLRQATAPVRTSSPMLRARMNALRLRCKMEIAAGLSSESLAEQSWACGKVRSALQGEDQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFF2</accession>
    <name>A0A9W8YFF2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002956</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFF2"/>
    <dbReference type="OrthoDB" id="94039at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="18"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="326" mass="36696" checksum="8D08A3E092335D32" modified="2023-11-08" version="1">MANQGASYTLNAEELGDDPSWIDHSLDLLLMINKFRSRMKTPLIGVAHSMGCAQIAYLASIHPRLFHSVIFIEPVMQASHPPGPNSALFSSMRREKWESRAKAEAQISKNRFFQSMDPRALKMFLAYSLKNTPDGGVTLSTPKAQEAWSYVRSNFHPLSQDTTEGRRRERMLAPDAVPFSGPGKAMTMRPEMLPTCEALPHLRPTTLYLYGEYSHINFDEVREQHIAKTGNGRGGNGGVNEGGVVEKILEDCGHLCVFEKPSTIAKDVSHWLDKEVAKWRKEKEFWDTVDTGKSKNGRKELSYKWMAVVKQDTTIERPSTKEAAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFG4</accession>
    <name>A0A9W8YFG4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001558</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the free Met sulfoxide reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFG4"/>
    <dbReference type="OrthoDB" id="15735at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033745">
        <property type="term" value="F:L-methionine-(R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.40:FF:000048">
        <property type="entry name" value="Free methionine-R-sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000614">
        <property type="entry name" value="FRMsr_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051330">
        <property type="entry name" value="Phosphatase_reg/MetRdx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021:SF15">
        <property type="entry name" value="FREE METHIONINE-R-SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021">
        <property type="entry name" value="GAF/PUTATIVE CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01320">
        <property type="entry name" value="UPF0067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAF" evidence="2">
        <location>
            <begin position="66"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4376275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="179" mass="19197" checksum="504CACEE762A79D4" modified="2023-11-08" version="1">MVHADSSAFADGLSKKEVYAQVLEQAKLLFEGQRNWARANHSNFSNTASLLWHAYHSLPSPSAHVNWAGFYFTDPSNPSQLLLGPFQGQVACQVIAFGRGVCGTAAEQGKTQLVADVESFPGHIACDGASKSEVVVPVVKEGKVVAIIDIDCAELNGFTEEDQAALEELAELLAQSCDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG59</accession>
    <name>A0A9W8YG59_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001683</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG59"/>
    <dbReference type="OrthoDB" id="2333384at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="64"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="401"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="404"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="441"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4376400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="453" mass="50319" checksum="3294A5CC10718849" modified="2023-11-08" version="1">MPNNLDQSVEGLPTNWVVYNLKASVDRGYMSKQLSATSHIRVIRTLGRDMLESMPMEQINEDIWANKLAYKITVPQKNYIIGTNITADFVLIPLRKGVEIATIKMELIESRQLFADYAGRRISHQTDIQVAVTEGEMPENSARTIPDGVEDPDELFDESHRFQMSLDLPKSLKNCRQSVDTENIRLTHKLRLYVNLKNPEGHTSQLLVKNHVHLFISPNLPPNEDQSVVVDNNILNAQAIQDEVNQNAPPTYGLHQLDELYNDIDPSGFMTPGGYHSIMNSGANTPFYAHSRSGSAEDLASLDAVAQQPGGGASAAALEHRLQNLDMSHHDRPSRFQPSRHHSSGDSTPAHNGEPSYFDLPAANYDMDALARTPSYNTALRTPISRTPGGVVDLDLPSYEIAMSRPGSPSRSRGSRGTTPSPARSLDTLNEETYRNTQINSRRESPRTSDDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG82</accession>
    <name>A0A9W8YG82_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Purine-cytosine permease fcy21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">FCY21</name>
        <name evidence="11" type="ORF">N0V83_000340</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG82"/>
    <dbReference type="OrthoDB" id="2116389at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015851">
        <property type="term" value="P:nucleobase transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11484">
        <property type="entry name" value="SLC-NCS1sbd_CobB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4160.10:FF:000002">
        <property type="entry name" value="Purine-cytosine permease fcyB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026030">
        <property type="entry name" value="Pur-cyt_permease_Fcy2/21/22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806">
        <property type="entry name" value="PURINE-CYTOSINE PERMEASE FCY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806:SF1">
        <property type="entry name" value="PURINE-CYTOSINE PERMEASE FCY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002744">
        <property type="entry name" value="Pur-cyt_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="283"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="327"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="412"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="492"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR002744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4377515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="518" mass="56649" checksum="A6DF6C6EA3AE0939" modified="2023-11-08" version="1">MLHDKYDHDIEKDAMRTTGPHDAIDDGAVPGESFEIGNSTYAKLQRLAGKFNIEQRGIERVPENERTDTSLLNVGTMWLSANMVVSSFAIGLLAQKLFYLGFVDAVLTILFFNLLGVIPVCYFSTFGPRFGLRQMVLSRFWFGWHGVKIIALFNVLACVGWSSVNSIVGAQLINAVNPDVPGYAGIIIIAVLTFFVCLFGYKIVHAYEFWSWIPTTIIFLIVLGVFAHSGAFINIPMGVGTSELGSVLSMGSVVYGFATGWTSYAADYTVYQPSNQSRTKVFFAVWIGLMIPLLFTEILGVAIMTATTMNGGDNRYMEGYTASGTGGLLAAVLFPPLGGFGKFCLVVLALSIIANNCPNIYSVALTVQVLGGRWTQRVPRFVWTGVATAVYIAISIPGYSHFEEVLENFMNFIGYWLAIYEGIAFADHFIYKRSMNAYIPAHYDQPRMLPPGIAAVFAFCCGIAGMITGMSQTWYVGAIALHAGEAPFGGDVGFELGFLFSFVAYAGARFAELKVFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGC7</accession>
    <name>A0A9W8YGC7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGC7"/>
    <dbReference type="OrthoDB" id="2408430at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="302" mass="33483" checksum="9655A38FFCF5C448" modified="2023-11-08" version="1">MVQGLLPKPLPADVLVVGQLLTHPLHPERDSFYPDKAHAEIDDLNDFHIQLRYKDLFSVDTEGRFMTNYGAKFDLGRIYRQPNLLTVKAEQMIQRTSQSPSQVFQAVCNDPEARAWIYEQVTAGKPLYVVLGLTELKNAVFKRGKLRDAGASNRLNETPIEKDANVPKPLRGRSESNLGGLGTEPHISGVFGMDVRRVMARVTTPAEPHSLEDIGYRWAYHDVPNSTNKEQLMIGLGKQLKADELRLMLDLSEEDVQGNLDAISQLSLDALSAAGSPMLLPSSPALRGRSPSPNPAMLRHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGG2</accession>
    <name>A0A9W8YGG2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGG2"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="94" mass="10334" checksum="9BC8CC8116406FB2" modified="2023-11-08" version="1">MAPEATQPVTTVGILGRKKDGAFILWSALFGSTVASLAFFARGHLDDSIEFHMEPDAFDMLESDEFWDREGGTVSARSPCSFTKLWVVDTSDSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGJ0</accession>
    <name>A0A9W8YGJ0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glyoxalase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001697</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGJ0"/>
    <dbReference type="OrthoDB" id="4179687at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4376414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="27840" checksum="0B738DFB20A5624F" modified="2023-11-08" version="1">MMHIIYEDPAVAQWGLKNFLVPLGGDIIEVVSPFKTDTTAGRLLEKRGDGGYMIIMQTEDARKRREYIEAKGLAKVIWGYDHNDTVCAQYHPKGIKGGMMPELDSHEPGPNNPTPLKSRFSPWHACGSDHKVYYPGMKRSEHLRLEGCVLRLQPGDAGHEAAARQWEEIFGVARSRDLLAFTNARLGFIPGREGQPEGLVSITVGVNSKEKFDALRTRAKQAGVYGNGCIEMCGIKWFFSLTGYGESAAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGL2</accession>
    <name>A0A9W8YGL2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">UFD4</name>
        <name evidence="9" type="ORF">N0V83_000372</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPL family. K-HECT subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="423283at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045322">
        <property type="entry name" value="HECTD1/TRIP12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057948">
        <property type="entry name" value="TPR_TRIP12_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25579">
        <property type="entry name" value="TPR_TRIP12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="8">
        <location>
            <begin position="1477"/>
            <end position="1834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="713"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1035"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1141"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1276"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="713"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="729"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="758"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1091"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1112"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1278"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="1801"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4377547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1834" mass="201704" checksum="1FB5FDF71B66ADDF" modified="2023-11-08" version="1">MASGLRESRLDHQRDRAATPNGVTRKTAPRDSSPEQPERPETAPARGGRAKRTKVEPQDSPAPAASRNKKAKGKAVMSSAGSVPGNPSTEEALTENSPPSDPIEEFSTPQPSSTKRGKGGRKSGGDSSRKQPKTPNTEHDPSAPSTSRRSSRRSTKADDDASMEDAPHSKPEEEPQQSGEDDESDDENAEGHGGRYDDEDDDDDDDPFSGFLGRHPHGLSALRQLTGMMAGTTQRLRGILEQLRSMDMSIQLIALQELSEVLLISTEDNLAGNFAPDAYVKELVKLMQPNEFTGEENPEVMLLACRCLANLMEALPASTANVVYGGAVPVLCSKLLEINFIDLAEQCLSTLEKISVEFPSAIVREGGLTACLTFLDFFATSTQRTAVTTAANCCRNIPEDSFPVVRDVMPILENILNNNDQKVLEQGCICVSRIVQSFRHQESKLEELVSTGLLKAILRLLLPGTTNLIGPNIHTMFLQVLAYTAKASPRLSAELLKMNVVDTLYQILTGVSPPSGTEDVAAKIDSVVIMQALIHRPKDQVYETLNVICELLPDVPRDGLMYLDDLFDAGYPGDDLRPLASNNKLTANEKRVELLEECKSEVKRFAVILLPTLTDAYSSTVNLSVRQKVLTAHLKMLSNLDTDILEEALRPVPYASHLASIFSQQDHPSLVTYALQAAELLLKRLEPIYRYQFYREGVISEISHLANRPCKPLEIRTKEPKPTLEVEVAADSGASTSSSGDRNIEQETAQVADVEMHSEDDDEDDDQDDDQNEHSDNGDEDDESDSSSSSDNQAPPPVPNIEDIITLRAKKFIDVHEKDSDKPIRDKASAVLEDLKSLARELRECVLNKGAGNCMQLFTSLAAYFEGDALESITSYELKSSKIVEVLLELFSTSTSAKGIDPRSSFLEAFMGGNSQSKIKTASTASPATPFSILVHKLQDLLSRSEHFDVITVHQNSHDGRGSATSMLAKQLRLKLVADDESGIPKTYRNIMVSIHAIATFKALDDYLRPRISLAERPRPTKTRDQFAAYAQAIAEGRAPPPPHTPSDSTNRLSKKSSRSKSGSTDSPQPGPSSATREKTRRSSRRQQAQLPPPPPPPPAIPQSNSSQDPVECADEEQLSDQDSLDDHSALDAIVDDLEDDLDEDVPDPSAVSVEVAPTGQATARKEDGTRIATPVQGTPIAKPPTENRTPSSRLAQLLQSSALRQGLGGAASALSYAAAVQSIPQDWHIEFSVNDQPLSNETTIYRAVHFNQAQPSDISHRTVWNAIHTIKFKRVQGPPPAESSSLSPPPDSKADALGMPASLGQHPVTSGILRLLSILHSLNSHLDDILLDNKEQIKVNAEPLSQFVNTKLTAKLNRQLEEPLIVASNCLPSWSEDLARFYPFLFPFETRHLFLQSTSFGYSRSMTRWQNAQPASDSRHDRHRDERPFLGRLQRQKVRISRSRILESAMKVMQLYGHSASVLEVEYFEEVGTGLGPTLEFYSTVSKEFSKKKLKLWRENDSNDKDEYAFGKRGLYPAPMSAEEASTENGEKKLELFKVLGKFVARSMLDSRIIDVSFNPTFFRIGDGSDTAVTPSLGAIMAVDQDLAKSLKLLKHFADAKQKIDADSGLSAAQKAEALQSIVIQDCHVEDLALDFTLPGYDSIELIANGSETPVTIQNVDTYVDKVLDFTLASGVQRQADAFRAGFTEVFPYSALKAFTPDELVMLFGRADEDWSLETLMDSIKADHGYNFDSKSVRNLLQTMSEFDMQQRRDFLQFITGSPKLPIGGFRALTPMFTVVCKPSEPPYTSDDYLPSVMTCVNYLKMPDYSSIEVLREKLSVAIREGQGAFHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGM4</accession>
    <name>A0A9W8YGM4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000387</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGM4"/>
    <dbReference type="OrthoDB" id="3935170at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="95" mass="10096" checksum="5489A7F0443D1FD3" modified="2023-11-08" version="1">MPALPFNSATPSPSTSPTLSSATPSIVLHPASGASSKRELHKSKEDAAEAYRLRYAQYLAATFNMSIQAAMAEADSQLAPRRSSSVSEAESLREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGP5</accession>
    <name>A0A9W8YGP5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Polyketide synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_001675</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05195">
        <property type="entry name" value="enoyl_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000019">
        <property type="entry name" value="Polyketide synthase type I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.366.10:FF:000002">
        <property type="entry name" value="Probable polyketide synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013217">
        <property type="entry name" value="Methyltransf_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032821">
        <property type="entry name" value="PKS_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020807">
        <property type="entry name" value="PKS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049551">
        <property type="entry name" value="PKS_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049552">
        <property type="entry name" value="PKS_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013968">
        <property type="entry name" value="PKS_KR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF29">
        <property type="entry name" value="ASPERFURANONE POLYKETIDE SYNTHASE AFOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23297">
        <property type="entry name" value="ACP_SdgA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16197">
        <property type="entry name" value="KAsynt_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08659">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08242">
        <property type="entry name" value="Methyltransf_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21089">
        <property type="entry name" value="PKS_DH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14765">
        <property type="entry name" value="PS-DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00826">
        <property type="entry name" value="PKS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="11">
        <location>
            <begin position="8"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="12">
        <location>
            <begin position="948"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="10">
        <location>
            <begin position="2521"/>
            <end position="2598"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="9">
        <location>
            <begin position="948"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="9">
        <location>
            <begin position="1112"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="9">
        <location>
            <position position="980"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="9">
        <location>
            <position position="1180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4376392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2606" mass="285817" checksum="CA22C33192E96020" modified="2023-11-08" version="1">MTSEDPASTPIAVIGFGCRFPGDVTSSEKLWEMLVEKKSARREVPTDRYNVDAFYHPDGDRYGTSNNRGGHFLAEDVSLFDAPFFSISPAEAMAMDPMQRLLLEVTYEALENSGTPLSKVSGSKTSCFVGCFTKDYEEMQRRDMELAPKYQSTGASQTMLSNRLSYFFDLKGPSVTLDTACSSGLVAVHLACQSLRTGESSMAVVGGSNLILSPDIQIEMSDMHFLSPDSISYAFDDRANGYARGEGVGTVILKPLDLALRDNDPIRAVIRGTASSSDGRTPGITMPSQEAQIDLIQSAYRAAGCDVSETGYFEAHGTGTAAGDPIETGAIGKVFAARRPLAANGEPIPLSIGSVKTNIGHLEGASGIAGLIKAVLSVEKGTIAPNIWFQRGNPAIDFEGWRLQVPTEAKDWPLEGVRRASVNSFGYGGTNAHVIVDDASHYLQKHHRARGHIRNDSGFVSPTSDTSVSATTRSRIFLLSANDERTLVQMGQTLADHLQAQSVFNEEELLDNLAYTLSERRSRFSYSSIIVAKEKAQLEVQIGKPVPISSARSTPPRIGFIFTGQGAQWWGMGRELLQYPVFKNCLQRCDSIIKKLGSDWSLMEELLKDKTTSRVNEATFSQPLCTTLQIALVDLLSSWNIRPQSVVGHSSGEIAAAYATGALSIESAVQVAYYRGLLSPKVKSLGYHGRMMAVGLSEAEAKGEIAKLDDTLGKVVVACVNSPRGVTISGDTHAMEKLLDALVAKGIFARMLQVDTAYHSHHMQAIADDYLDRLREARVVALDDQPRATMVSSVTEEVVNHDQLGAEYWVSNLVSSVRFSGALEKLCLLQSGDGEKGVDVLLEVGPHALFKLPAKEILEATFGEKSNIQYFSTLIRNKPADITALEVAGNLFASRYPVDLHTVNFPVEPKQPLAVLTDLPQYPWNHTRGYWCESRLSRDYRLRKFTRTDILGAPVYDWNPLEPRFRNFLRLREQPWLRDHVVQGDVLFPACGYLCMAIEACRQMCVISPTTFMPQNSNAPVEYRLREVSISRALVVPETDEGVETSFSLRCQSIGDSSSSDVWHEFRIFSYTVDGGWVENCRGYVSVSTQPTIDDELSKDCLDEWTTRTQKAQNVDVNTFYNTVDALGLTYGPLFQGLKEIQLKTTSPDQAVGIVEVSNTRLSNPKEFEHDRLLHPATLDSFLQLALAALGGAELKQLTGAMVPTFVEEITISTNIAAQTGDRLHIVVNSERQGSREAKSNFFALDPVTGKSVVLMDGFKFVAINSASDSSESHPIAKHCFKPVWEPDVELIDRQSLDRALQAAPRPDDRPKTVRELELVSYYFIDQALQDVNDSEVAAMLPHHQKFYRDLCRLRDTVLAKTHPQQTDEWQQLHTSEVATKLRSMVDHYRFHETAYDGKLLVRVGEALPSIFRQEVEPLALMTHENLLEDYYTTAVGMPNTYAQISRYVTLLSHKYPNLDYLEIGAGTGGATVPTLQGLSGCEDLHTYPRLKSYTYTDISSYFFQRAAEKFETFVQFMKFKKLDVEQDPETQDFRPASYDVIVAANVLHATSDMHRTMVHARKLLRPGGKLILLEMTNRLLAASVIFGTLPGWWNASDGRTGGPLLTETQWQDVLQATGFSDLKASSPDVLEPLEEGTRLMIATAVEAKSTICNGLSTPPGSPRILLLCSDSPIKMSRLDIPLAFQAKMQGEGLQVELVPLSMLAQQDFINTVCISFLEVEEALLAIPSPSQLQTLQRLADTAAGLIWVTRGAAASNVQRPELAVFQGLARTMRAEHEGFPCITIDLDANEKLTTQEVANLLFHVYEQSLSPGKSSQVVDSDLIEQDGVIHIKRAIEDEQSNQFLTSRTAPDTLPPQLENLVQADRPLRFSHSEISLKGPFVFEDDLLTAQPLQPDEVQIEIQATGLNSKDIRVILGDLSNETPGQECAGIITSTGSSVSHLSVGDRVVAWCAGAFATHLRTTAALVHKIPDTMSFEVAATSPVAYITGYHALIHVARVAACEKVLIHEAASNLGQAAVQIALDRGAKVFVTVRSEEEKTCLSDVFGIHPSHIFSSQDTIFVTALRRLTKGRGVDVVLNTLSGEALQATFSCIAPFGRFVELGKTNSESNGRLEMAPFERNVSFSSVDVELLRSHNTSLASNVFQEAMSFLVHRGVQNLLPVELKPWSELGEGFAQMQDEKFLGKIAMVPRADDRVLVSNWFPSPEYRIANDIKVVQKPPKQISFDSDATYLLAGGLGGLGRSISHWLTKHGARNLVFISRSGASSPEAASFISSLESAGIRTAVLQCDISNAFALSTALSPTLAKFPPLRGVIQGAMTLNDSLFSNMTHAQWTSTILPKVHGSKNLHEATLSQPLDFFILLSSLHSFIGNPGQANYAAGCAYQVALAQYRNKLGLPATAIDLGIVGDVGYVVEKAAKKEKLRVQEFKHIKEEEMLALIELAIREPKSGHIVTGLDSDFDLKEWEAEDSTPFFAKDPVLSHLDYLRPHLHTSSTTQTSTDGAVSTTLSLSAQLASAPSAEQAKNYILTALLKKLSRSLMMDPSELDATRSMSAYGTDSLVAVEMKNWIQRETKVGVSVFEILQASSIGGLVEKIAERVSGGGKGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHL2</accession>
    <name>A0A9W8YHL2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ustYa family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHL2"/>
    <dbReference type="OrthoDB" id="3687641at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043386">
        <property type="term" value="P:mycotoxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021765">
        <property type="entry name" value="UstYa-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33365:SF14">
        <property type="entry name" value="TAT PATHWAY SIGNAL SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33365">
        <property type="entry name" value="YALI0B05434P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11807">
        <property type="entry name" value="UstYa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00035112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4378179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="329" mass="37597" checksum="1A97F6BAA6908E14" modified="2023-11-08" version="1">MNFLPKFTYKPVLSADTRDGSLESDDEETKGFLHGIPPQGSIRTKAISRTAILFTILNTCFFLISSTLFATWFFNTHLLLNSSYRLITPYSPIHSQFDLAPTLKRINGAFYNSKNASLPIARQQPNPAADAIWDEWELTRVYPATRADIIKMGSDPSTVAKLEDDLWGLGDDAYATIFDVYHHVHCLNSLRHMAYGTYYNLSMARAHTIKQREIHINHCIDILLQGIQCNANLDLIPLHWVETQQYPFPDMSINKKCVDFEGLTQWRKENTIDMERYVEVMKRPAGDKPGIKQHPAADQYYEYWGYENPNHKPVAEGGHGIPLDEDSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHP7</accession>
    <name>A0A9W8YHP7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000965</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHP7"/>
    <dbReference type="OrthoDB" id="3790567at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4378132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="232" mass="26629" checksum="B099FB3A75173E18" modified="2023-11-08" version="1">MLSFNIIFNFLLGLVLSAVVVWGSEELRIAVGTVSSWALGHLLLRRFAALPAGFRRAFHAYLFVSFWLLAAYNDIFWENVFGRVLDLWFPRRSPNDCPGGGFTYWVYLFRRRHAYEIAFACRFLRFAVCSVLAAVFMVLAVPLVHWQVKVYREQARLERSFAYLHEYGELPPWDRVQPPVEIPPPKRDTRYMRLSDDIVFVCGPGGGFATVVAGVLEDSPPSRMPGHYKVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YI76</accession>
    <name>A0A9W8YI76_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_000818</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YI76"/>
    <dbReference type="OrthoDB" id="9983560at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050432">
        <property type="entry name" value="FAD-linked_Oxidoreductases_BP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13878:SF91">
        <property type="entry name" value="FAD BINDING DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G12070)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13878">
        <property type="entry name" value="GULONOLACTONE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040787598" description="FAD-binding PCMH-type domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="4">
        <location>
            <begin position="125"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4377988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="598" mass="65482" checksum="F302F615C12162B4" modified="2023-11-08" version="1">MKLLPAFLSYLPSSSFLASATEQCKCTPDNKCWPSDAEWSSLNTTLKGKLIRGVPPGSACYPEQPNYNQKTCAFVASQWFNSTWHATNPVSIDYPIWTNNSCNPIWPDGTSVTGDVNAGAKGCSFGAYPAYVVNATTAEEVGAALKWAGEKNIRVIVKNTGHSYPGRSIGYGSLSIWTHNFRGIEYIPAFNPTSCSMNGTLTAVRIAAGHTGVEVQAEVAKNEAIIVTGANPDVGLVGWLTGGGHGALSRTYGMGADNLLEATIVTPDGRTLLTNPCNNADVFFAIRGGGGGTYGVVTEVLVKAYPTPNMTAQTFWVTSLSPDTSIEFWDLVGFVHAEMQRLKDGGMQGYYQLGGLPFVPTLSLQWNFFLYGKPYGTVERLMAPIEEYLEQRTDLFAYESDIAYADTYLDLAGGAMNELVANGGSAYGSRLLSPRSVSNASDVARMFKKIWASTDPLKPNGPVTNPYFIGHMIAHPDAPTYYPDVISMNPAWRNTIAHLIVVEGWQDGMPQSIIDAVYDDITYNKTEALRKLSPETGAYFNEPDSYEPEWQQAFFGEHYDRLRSIKEKYDPYNVFWCRRCVGSEALIEQPDGRLCSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIW8</accession>
    <name>A0A9W8YIW8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">L-ornithine N(5)-monooxygenase</fullName>
            <ecNumber evidence="5">1.14.13.196</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">L-ornithine N(5)-oxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SIDA2</name>
        <name evidence="15" type="ORF">N0V83_001712</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-ornithine + NADH + O2 = N(5)-hydroxy-L-ornithine + NAD(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:41512"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78275"/>
            <dbReference type="EC" id="1.14.13.196"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-ornithine + NADPH + O2 = N(5)-hydroxy-L-ornithine + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:41508"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78275"/>
            <dbReference type="EC" id="1.14.13.196"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Siderophore biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.196" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIW8"/>
    <dbReference type="OrthoDB" id="3519933at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019290">
        <property type="term" value="P:siderophore biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000195">
        <property type="entry name" value="L-ornithine N(5)-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025700">
        <property type="entry name" value="Lys/Orn_oxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42802:SF1">
        <property type="entry name" value="L-ORNITHINE N(5)-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42802">
        <property type="entry name" value="MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13434">
        <property type="entry name" value="Lys_Orn_oxgnase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4376428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="548" mass="62038" checksum="B16B0AC05A723C81" modified="2023-11-08" version="1">MGSRLLPSYLPYLRRASSSMSPHAEISSSSPESSAPAVQKAFDGFLSNDLEESHFPAFENRSHLRYTPEEELHDLICVGFGPASLAIGVALHDALDGTDPSLVDVPGLQSRPPRVAFLEKQSQFAWHAGMLLPGAKMQITFMKDMATMRNPRSEFTFINYLHQKDRLIEFANLNTFLPARVEYEDYMKWCASWFEEVVSYDQEVISVMPEKSSSGEITTFNVMSRNNQTGRTESRRTKHVVIAAGGRPNIPKPFPTNHPKVVHSSKFSYISKEILRDHKAPYKVAVIGNGQSAAEIFDFLHANYPNSQTRLLIKGGALRPSDDSPFVNEIFNPQRVDCTYSREPKLRTMTLVEDKGTNYGVVRLNLLEHIYETLYMQRIRHGNSPEEEAQWPHRILPYRRVIDVEDSPVQQGGIRLHVRDQSPLYFSEVPGAQEQTEILDVDAVFVATGYFRDLHETLLKDARHLMPGGDLKDAKWAVQRDYRVNFENKKVSEDTGVWLQGCCETTHGLSDTLLSVLATRGGEMVRAIFEKQPKWDSADVLGGYEERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJJ4</accession>
    <name>A0A9W8YJJ4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_000514</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJJ4"/>
    <dbReference type="OrthoDB" id="10262656at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF2">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04150)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="337"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="370"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="439"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="466" mass="50135" checksum="452CCF1F9B27498D" modified="2023-11-08" version="1">MKILKMLPTADFLPHNCSSSLVSNFHIGSESNAPFFAGLLVSAFSLAEACSGMYWGGVSDRVGRKPVVILGCIGTLSSLLLVGLAPNFWVALAGRIIGGALNGNIGVIQTMVGEIVKRPEHERPTIGGYFAEPAQNFPSLFSSTGVFSKFPYLLPNLICSFLLLLSIVMAYFLLEETRSDSQTWGRFEESGGAVADTPLLPAQGATADAPADLTTESYGTFNTVEVQHDEVWRVRSNGDWVEPPASEKVFTKTVLQFTIALGIFTYHSMTYDHLLPIFLQDKRADDDINALDLASASLGGGLGIPIQNVGIIMSFNGVIQLAIQALIFPPLADCFGVWRLLLMVTVAHPIAYFIVPFLQLLPANLLYPGLFFCLAVRNLTSILAFPLLLIMIKEAAPDKSHLGKINGLAASTGAACRTVASPIAGLLYGLSIEMHFTPLAWWCSALVAIFGALQVPFLVSKAKSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJL1</accession>
    <name>A0A9W8YJL1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Leucine-rich repeat-containing protein 40</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000966</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJL1"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF54">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4378133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="483" mass="52808" checksum="71DE9E0BE1A346BC" modified="2023-11-08" version="1">MAPVLEVIAQIPTLRELKLAENDLQGDLSTTLCKLTSLEVLELQGNKLSSLPDAMGQLTLRTLNISDNQIRTLPMGLFTSSLVELHASKNRLEGTLFNVDSVPSLQVLHVANNSLVSLHGSNFIQLPALKVLDVSTNRLTSLPDVTTWTSLVALLVGENKLTTLPEGFVTLSVRTADFTGNDITQLEERIALMENLEHLTVAANPLRERKFLTMSTEEIKRNLASRLQVSDTTVTGLDEELDPDGNELAQGSKWQLTPSGTLDLSGKDLAELELDEATLGAFANEARQVHLQQNVLNAIPAVLFHLTHLAVLDLSKNSIEIALTTPLELPKLRELRLVSNKIKSFEPLTSHLTAPSLQILDISQNRLSGALPTLHSSFPMLLTLLASDNSISDVSAESLTGLRVVNLSNNDIERLEPRIGLLQGTLTALEVGGNKFRVPNYQILGKGTDAVLTWLRDKIPRESWKSDATTEAEFFDADDGTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH18</accession>
    <name>A0A9W9CH18_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">O-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_010815</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH18"/>
    <dbReference type="OrthoDB" id="3340390at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF17">
        <property type="entry name" value="O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4361874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="185" mass="20692" checksum="8336740892097782" modified="2023-11-08" version="1">MERFMFDDFLNVGWKVPSFLDSISHRNPADINNGPFQHAHNLPGKSLYTYFGEDTAMGERFGGMIQMYNAGKPFFWDDGYYPIKERLVEGGPDSVDDVLLIDIGGGDAGDLGLLRKALGPEVKGRLILQELKHIVDRSDPQGYEAQVGDWNQVQPIKGTYQAKSHTSVSSTIPWKHHRKAAVTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH38</accession>
    <name>A0A9W9CH38_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010340</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH38"/>
    <dbReference type="OrthoDB" id="417891at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43180">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43180:SF63">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE FAMILY PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G03520)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="137" mass="14694" checksum="A6E17CC0D850DA7D" modified="2023-11-08" version="1">MGPNECSGIGLETVILMLREGASVLMTDIAAPALDTAITKAKEVVPSHKGRVETRVVDVTKESDIEAAVAYIDPWGGVDVMFNNAAIMHPKDGDADECPDAIWDLTFSINVKGVWYGSKHAVRSLRKHGKKTGSIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHE6</accession>
    <name>A0A9W9CHE6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">VLRF1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_009938</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The VLRF1 domain mediates binding to the 60S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ANKZF1/VMS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHE6"/>
    <dbReference type="OrthoDB" id="429841at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047139">
        <property type="entry name" value="ANKZ1/VMS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041175">
        <property type="entry name" value="VLRF1/Vms1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16036">
        <property type="entry name" value="ANKYRIN REPEAT AND ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16036:SF2">
        <property type="entry name" value="TRNA ENDONUCLEASE ANKZF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18826">
        <property type="entry name" value="bVLRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52044">
        <property type="entry name" value="VLRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="VLRF1" evidence="13">
        <location>
            <begin position="235"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="475"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="28"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="283"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="405"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="530"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="408"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="534"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="561"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="624"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4363642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="663" mass="72570" checksum="2CE21014764ED0C7" modified="2023-11-08" version="1">MAQKGDHLLQRPLYVFDLPEELLATLTLKDQSARPVQDTETSQQKPPPDSSDVEDGPAKATSCNLCGLSFASVADQRSHVRSDLHGYNLKQKIKGAKPVGEADFEKLIGDLDESISGSESSESEDDEAEEDGSKAKESTLSALLKKQAKISNPEFDEFSSRKKQQGAGKPPLIWFASPTIPDNMSLGVYRAIFSVDEQAEESHIIDTLRKKQLSPKQPPKVKANEGGVPLPGTDIGPHYFLCMIGGGHFAAMIVALAPKTGKKHTGADERSATVIAHKTFHRYTTRRKQGGSQSANDNAKGNAHSAGSSIRRYNETALVNEVRELLSNWKSLIDTAELIFVRATGSTNRRTLFGPYEGQVLRNNDSRNRGFPFSTRRATQKELMRAFVELTRVKQSTIDEAALAALNAPQPDTTTTAPAPAKPKPPKPSKEQELAALHTSQIIPLIKRSKVPALLNYLKTNNIPSTFTFYPENHHTPTPLHLAASLNSAPIVLALLTKASADPTLMNDDARTPFTLAGDRATRDAFRVARSELTSSSTSTPTTSWDWDLAGVPPPLSKAEANARDAQEKSEREAESKAESERRKAEAERLRKEGEAEEARRREGRLGKGKALGAGGMGLVKSGAEVREEEMRGLSDEARARMERERRARAAEERFKRMQGGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHK4</accession>
    <name>A0A9W9CHK4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fatty acid desaturase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010006</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHK4"/>
    <dbReference type="OrthoDB" id="1461976at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32100">
        <property type="entry name" value="OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="2">
        <location>
            <begin position="78"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="296" mass="33519" checksum="151D880359B28432" modified="2023-11-08" version="1">MGAITTATQLQVSTEPADSVVPDVKTLRDAIPAKCFERSLLRSMSYVVRDLIAVSALFASAVWLSKLDAPWSVTIPLWTLYSFVQGLFFTGLWILAHECGHDSFSPYLNVNATVGWFLHSILMVPFFSWKFSHSRHHRYANHMQKDTVFVPSRKEEVEERESRGVSLVEKLIDHTAADTPILTLCALIFHQLLGWPAYILLNAGAGMKSLTKNSREETSTFKQSHLDPTAHVFTDGEQPFVLLSNVGLMLVLTVLYQVSQYLGVGHTFLLYGLPYLWMNHWIGEFFRHSWKADANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHR7</accession>
    <name>A0A9W9CHR7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MOSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010204</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHR7"/>
    <dbReference type="OrthoDB" id="17255at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005303">
        <property type="entry name" value="MOCOS_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SULFURASE MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237:SF23">
        <property type="entry name" value="MOSC DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_7G05900)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03473">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03476">
        <property type="entry name" value="MOSC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="MOSC" evidence="2">
        <location>
            <begin position="285"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="463" mass="51513" checksum="5413B94C4923E938" modified="2023-11-08" version="1">MSTNVFDHFVTATGLNNISPVAIFTTVLVAVLPVVALLILALTQADVPLPPPAGCRKLGIKGKSNLEDQYSKRYAKGGDPTPEKPWTVKALFVYPVKSCAPVEIEQSEVIRTGLKYDRQFALGQHVTSLPSMDGKVTSEWHFLTQRKFPRLAKVETEVWIPDPSAKGYKEDGEWVKSEGCVVVRFPFSPDTDFTREGLLNYGKVLAAKLAGKAEPTLEFRVPFNPSKDAIKSKDYKNEVLRIWKDQPVALNMSSAVEPEVLSKLRYTLGTTNPIALFRIDTNKYREVHKCAPKKEEVGFQPVIGMQDSYPIHIMNLASVHHVAENLPVKKSEFQWQNHLTLLNALRFRANIYITGPPAFHEDDWAKAKIASPSNSTSTTYHISCRTTRCKLPNVDPESGVADRNEPMTTMRKYRVIDEGSKSACLGMQITPLEDGEVKVGDHVEVLETGKHHFLAGEGSKVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CII3</accession>
    <name>A0A9W9CII3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Jacalin-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CII3"/>
    <dbReference type="OrthoDB" id="74460at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.30">
        <property type="entry name" value="Jacalin-like lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036404">
        <property type="entry name" value="Jacalin-like_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053002">
        <property type="entry name" value="Metalloproteinase_M10B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021917">
        <property type="entry name" value="Unchr_Zn-peptidase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21054:SF2">
        <property type="entry name" value="MIP04191P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21054">
        <property type="entry name" value="ZINC METALLOPROTEINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12044">
        <property type="entry name" value="Metallopep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51101">
        <property type="entry name" value="Mannose-binding lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51752">
        <property type="entry name" value="JACALIN_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="2">
        <location>
            <begin position="635"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="785" mass="86995" checksum="E6803CD8DE603A45" modified="2023-11-08" version="1">MPSFLHNLRRKSSRSAKAFEKPGQQVVDGQANGQNGKGLSHRVSASTLSSSQVGSSTPSTTPATSTSEDAAAHTKDNTYGPTPLPSRPQRPSVDPVKRYSMNGLSSPTSNGSNGSINKASLLAPRVLSVSDNSWVHQQVLLVFGQIGEANSRPLDGTITVNHHQGRFPPTAWPVHDSYFKALVHLEPGWNRIRLDFSSPKVSTSNTSVPSHASFVNINYLPLSHSPPLQLAIILGRDSPGTYDAPPERMQREGNGLDLAIRKFRMAAYLWQSFTGEQMKRYGFGRRCFRFEDSWEPGTLSYQDWGTNQYRTQAKIHVIRSNKTVAEIQDLERAQQYGPGTKKGELFDIALEACRDYFHITPGQKHYVSCLFLDSHWDTKEKVIRGHAALGGGAGDLQIGIFGSHALHSYPSSIEEVYQAFQDCTRTDAALIANDCNESGSSWEAANIGIGAHLHETGHLFGCPHQESGIMLRDYVTLNRTFVCREAFSTRTKSPGQKLVLPHDECRWHKLDVLRFRFHPCFRIPIDQDNIHGDSSVQVWTVDSGQGGVIATSKSGIAWIELYPEGDDVCHYWKEFPESDNQFPRQVVLDESVLRALMPKEKQKSRLKIEVFSAGGGQHVVEDFSQLANQKLARVKIPDGRWGYRGSKLGYSQMEGSEPQEIILEHAYIQTKLLLSIRVYHGYAIDGLEFIYEDAESQLFGKRGGSPSDFPLDTRKGELLLGFALRAGLWIDGIQILTTLGRKSDWFGNPNGGSGHTLIPPRGYTIGGISGSCANWLDGFSLIITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIQ8</accession>
    <name>A0A9W9CIQ8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-dehydropantoate 2-reductase</fullName>
            <ecNumber evidence="4">1.1.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ketopantoate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_009186</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16233"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.169"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.169" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIQ8"/>
    <dbReference type="OrthoDB" id="3609at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008677">
        <property type="term" value="F:2-dehydropantoate 2-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000017">
        <property type="entry name" value="2-dehydropantoate 2-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000609">
        <property type="entry name" value="2-dehydropantoate 2-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003710">
        <property type="entry name" value="ApbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051402">
        <property type="entry name" value="KPR-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00745">
        <property type="entry name" value="apbA_panE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708:SF26">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708">
        <property type="entry name" value="PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0521">NADP</keyword>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketopantoate reductase N-terminal" evidence="5">
        <location>
            <begin position="5"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="6">
        <location>
            <begin position="201"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4364590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="340" mass="37177" checksum="BC7D686CBCD64AC4" modified="2023-11-08" version="1">MPVEILIVGAGAIGAFYASRLALVSGTSVSVICRSNYKAVKGNGFQITSPQYGDYTFIPAHTFANPEEARQSGVEWDYIVVSTKALPDVSDDSAILEGLVGEKTAIVLIQNGLGVEGPYTKRFPQAAICSAVTIATCAQPAHGQIKHNRWTRINSGPYLPHLDTAGARESDARVTEQNNAFIALLKEGGIKDAEAYSHAKLQLVRWHKIAINASMNPSSVLTLCSTNYAMASDPELQLHLKGVMTEILETAPKILGQPMPKEFATPEQIIRSTQRNTSGSKPSMAIDWESRKKMEIEVILGNPIRIARERGFEMPRLQTLYALVRMAQEVRDKPATESRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJW3</accession>
    <name>A0A9W9CJW3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UDP-N-acetylglucosamine diphosphorylase</fullName>
            <ecNumber evidence="3">2.7.7.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">UAP1</name>
        <name evidence="8" type="ORF">N0V83_007757</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N-acetyl-alpha-D-glucosamine 1-phosphate + UTP + H(+) = UDP-N-acetyl-alpha-D-glucosamine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="2.7.7.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.23" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJW3"/>
    <dbReference type="OrthoDB" id="532420at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04193">
        <property type="entry name" value="UDPGlcNAc_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000075">
        <property type="entry name" value="Probable UDP-N-acetylglucosamine pyrophosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039741">
        <property type="entry name" value="UDP-sugar_pyrophosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002618">
        <property type="entry name" value="UDPGP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952:SF2">
        <property type="entry name" value="LD24639P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952">
        <property type="entry name" value="UDP- GLUCOSE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01704">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4366122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="483" mass="52671" checksum="71ED8622B1C09B9A" modified="2023-11-08" version="1">MEAVKDTINSALEALHITGGNQTVSAKEPSEEQLNDLKSKYAKAGQEQVFSFYEKLTTAEKAGLFEQLSDFNPDYINEITDRALNPAKSESTETKLEPLPENATSSVLDSSQENLDKWYSSGLDLIAENKVAVVLMAGGQGTRLGSSAPKGCFDIGLPSKKSLFQLQGERIAKGAAAGGEEARQGERHCSVENNYFGLKKENVVIFEQGVLPCISNDGKILLESKSKVAVAPDGNGGLYQALIQSGVVQDMGKRGIQHIHAYCVDNCLVKVADPAFIGFSASKNVDIATKVVRKRNAKESVGLILQKNGKPDVVEYSEISTEDAEAKDPKDSELLKLPFLESIPEWAKKLPHHVARKKIPYVNTETGETVKPEKPNGIKLEQFVFDCFPFLTLEKFACMEVKREDEFSPLKNARGTGEDDPDTSKQDILTQGKKWVQAAGAIVVSEEQKDGIEVSPLISYGGEGLDFLKNRTIKAPAVIESED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK37</accession>
    <name>A0A9W9CK37_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hemerythrin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007554</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK37"/>
    <dbReference type="OrthoDB" id="10044044at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.520">
        <property type="entry name" value="nmb1532 protein domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053206">
        <property type="entry name" value="Dimeric_xanthone_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38048">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38048:SF2">
        <property type="entry name" value="HEMERYTHRIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hemerythrin-like" evidence="1">
        <location>
            <begin position="2"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="168" mass="19752" checksum="56AF2F7FEE132782" modified="2023-11-08" version="1">MDHSGFRAEWDRIYATGNDGIRSMTPEAFMTMILEWCQSLDKHHNLEEQHVFPKLAVKMPAFRRDESDESRVADVVHANERLVSSPGYVHEQHRQIHAGLDVLHNCVKTWLAREQNEVDWEDTRKLMDSFGAILWKHMDEEVEMLGAANMKLYWTLDEMKQLPFKVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKK1</accession>
    <name>A0A9W9CKK1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7730 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007603</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKK1"/>
    <dbReference type="OrthoDB" id="62952at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056632">
        <property type="entry name" value="DUF7730"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF7">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24864">
        <property type="entry name" value="DUF7730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7730" evidence="1">
        <location>
            <begin position="11"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="171" mass="20237" checksum="A135DE5D63D82CE6" modified="2023-11-08" version="1">MQRSTPAPETTTSPFLRLPIELRRLVYQHVLPHTTTFDVRQQYPPYSAAPKTAVSLTFIRQKNGDGAWRMQKTASRVERETGHDIVWKRGRTNLLAVNRQIHEEAVDMLYGENTFVIDVTFDSINFRYRWRTRSDLTPNRTYQFLDHFSQRNLLRIKNYIINVESVDDYTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKY4</accession>
    <name>A0A9W9CKY4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein with SprT-like domain at the N terminus</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">N0V83_007099</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Spartan family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKY4"/>
    <dbReference type="OrthoDB" id="5236983at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006642">
        <property type="entry name" value="Rad18_UBZ4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044245">
        <property type="entry name" value="Spartan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006640">
        <property type="entry name" value="SprT-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055220">
        <property type="entry name" value="SPRTN_ZBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21220">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE SPRTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21220:SF0">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE SPRTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10263">
        <property type="entry name" value="SprT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22934">
        <property type="entry name" value="SPRTN_ZBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00731">
        <property type="entry name" value="SprT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00734">
        <property type="entry name" value="ZnF_Rad18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SprT-like" evidence="15">
        <location>
            <begin position="44"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="UBZ4-type" evidence="16">
        <location>
            <begin position="330"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4367516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="354" mass="39918" checksum="F359CB42829F9985" modified="2023-11-08" version="1">MPVTDEEAALAAIATLEDLTAEQQEALRAINTIIHNGEPFVDIHELSNHYNILYFRKLLLPRVEVLWSTRLTLCAGICELSKDVSTGKFTRIRLKLSTPLLQYRPRSDTINTLLHEAIHAYFFITTSWRHSRGDDGTGHGLGFQLLADAINNHGNYEVTIYHTFHDEVESYRTHVWQCDGPCQTRPPYFGLVKRSMNRAPGKSDSWWTKHEADCGGTYTKIQEPAPTKKQLEAMSTKQRAGRQKNKLDNWVKGGAKKSAETEGNTFDRPINVDVEGETSIKTHNRRVKESTVVVDDVVESEGSSTSSRAARKRSLVADDDQALSLEKKLLLECPICNLRIAESDINEHLDLVHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL04</accession>
    <name>A0A9W9CL04_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006726</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL04"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1000">
        <property type="entry name" value="arf6 gtpase in complex with a specific effector, jip4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="27"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="104"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="209" mass="23687" checksum="AD682EFE85DFB42D" modified="2023-11-08" version="1">MSHKRKREETRKEVEYDRKTKASGDEATTLRQQIDKLAAENAQVQARYDALAASNKQLEAQLNQIAAENERLRQQHDYDKNAYKGLAQKHNELSTRIAPTTTRVARLENTVVNLRTDLMLLRDRNKHAREEAEAAKKDKNIAEAALRDTKMTVAKDVAKINQWTTHLAKEAAALAKTSDMVAKRAEAHAKKYATDCAKKYAIDLTDEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM35</accession>
    <name>A0A9W9CM35_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b-c1 complex subunit 2, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Core protein II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">QCR2</name>
        <name evidence="14" type="ORF">N0V83_005448</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM35"/>
    <dbReference type="OrthoDB" id="6369905at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF209">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="12">
        <location>
            <begin position="54"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="13">
        <location>
            <begin position="201"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4369686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="459" mass="48655" checksum="562CD5B655934998" modified="2023-11-08" version="1">MFSRSTACRGAQRAARQHALRQTQRRGLAAPASGSFQYQTGEAKGVKYASRDLAGPTTTLALVAKAGTRFQPLPGLTEGLANFAFRGTERRSTLRIVRESELLGASLNAYHSRENLVLEAKFLRDDLPYFVELLGEVASSTKFQPHVYAEEVLPLIDAAHKRFLASVSAMATQSAHSLAFHRGLGTPTASASPTPYTKYLDAETIEYFSKIAYAKPNFAVVANGADHGEFSKWVGEFFDNVPASAVEGFSDAATQQTKYYGGEERIAHDGGNAMVIAFPGSSSFTGKFYKPEIAVLGSLLGGQSAVKWSSGFTKLSQAAAPGAKVKTTSAIYSDAGLLYTTITGSAKAVAATAKASIEAVQKIAAGEISSEEISKAKAAAKFTELENGQDTRAGLELTGNGLVFNTQAYQIDEVAKKIDGVSEEALKSAAKQLLEGKATVSSVGDLFVLPYATELGLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM82</accession>
    <name>A0A9W9CM82_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_005137</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM82"/>
    <dbReference type="OrthoDB" id="202203at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735:SF3">
        <property type="entry name" value="FERROPTOSIS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="5">
        <location>
            <begin position="5"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4370616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="373" mass="40507" checksum="22D58BE377DB8286" modified="2023-11-08" version="1">MKKKNIVILGGSYAGVSTAHRILKNVDKAAPFKITLVSPNTHFYWNVASPRGMIPGQLTDEQLFLPIAAGFGKYSADQFEFINGSAESVDTEGKTVVIATEARKVTLDYDMLIIATGTRTTERSPFKGVGSTEETRGLLHEFQEKIDKAKTIVVAGAGVTGVEVAGELGSEYGREKEIILVAAEATVLSVAPRHVSKLASQALQKVDVRLKLQTKIIDSIPRPDGKQELTLSNGETLTTDMYIPTFGLIPNSSFIPDRFLNTRGFATVDAHLNFKGTTDVWAIGDICDVEYCQILSCDRQSTYVAKSIVAILGGTGPAAPYKAMTSRMMGLQIGKKAGTGFYGSWRIPTFLVVRLRKTLFVERLGPMVDGSQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMA5</accession>
    <name>A0A9W9CMA5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UV excision repair protein RAD23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RAD23</name>
        <name evidence="9" type="ORF">N0V83_005660</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Involved in nucleotide excision repair.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RAD23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMA5"/>
    <dbReference type="OrthoDB" id="419317at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14280">
        <property type="entry name" value="UBA1_Rad23_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14281">
        <property type="entry name" value="UBA2_Rad23_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01805">
        <property type="entry name" value="Ubl_Rad23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000175">
        <property type="entry name" value="UV excision repair protein Rad23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.540:FF:000001">
        <property type="entry name" value="UV excision repair protein RAD23 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000002">
        <property type="entry name" value="UV excision repair protein RAD23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000003">
        <property type="entry name" value="UV excision repair protein RAD23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.540">
        <property type="entry name" value="XPC-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004806">
        <property type="entry name" value="Rad23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006636">
        <property type="entry name" value="STI1_HS-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015360">
        <property type="entry name" value="XPC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036353">
        <property type="entry name" value="XPC-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00601">
        <property type="entry name" value="rad23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10621">
        <property type="entry name" value="UV EXCISION REPAIR PROTEIN RAD23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10621:SF0">
        <property type="entry name" value="UV EXCISION REPAIR PROTEIN RAD23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09280">
        <property type="entry name" value="XPC-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01839">
        <property type="entry name" value="RAD23PROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00727">
        <property type="entry name" value="STI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101238">
        <property type="entry name" value="XPC-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3 5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="7">
        <location>
            <begin position="140"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="7">
        <location>
            <begin position="332"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="88"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="187"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="88"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="187"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4369896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="380" mass="40090" checksum="F4FDAA2DC7E43A9E" modified="2023-11-08" version="1">MKITFKDLKQNKFVIEAEPSETIGQLKSKIQAEKGWDVPQQKLIYSGKILQDANTVESYNIEEKGFIVCMVSKPKASAASSSKAAPATPVPAAAQTPAVPAAPAPSSTTTQNAPATPSPAPAQQASDRQFNDPSALTMGGEREAAVANMESMGFARADIDRAMRAAFFNPDRAVEYLLTGIPESAVQEQQQAAQARAPTSPQPATGGNTGATAAPSGGDEPINLFEAAAQAGQNRGGSGGARSGGTGGGAAAGALNANSLDFLRNNPQFQQLRQVVQQQPQMLEPILQQVGAGNPQLAQMIASNPEQFLQLLAEDADEDAPLPPGAQAISVTEEEREAIERLCRLGFERDLVIQAYFACDKNEELAANFLFDQPEDPDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMD1</accession>
    <name>A0A9W9CMD1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ACF2_2</name>
        <name evidence="12" type="ORF">N0V83_004616</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 81 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMD1"/>
    <dbReference type="OrthoDB" id="4473401at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052861">
        <property type="term" value="F:endo-1,3(4)-beta-glucanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1170">
        <property type="entry name" value="glycoside hydrolase family 81 endo-[beta] glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005200">
        <property type="entry name" value="Endo-beta-glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040720">
        <property type="entry name" value="GH81_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040451">
        <property type="entry name" value="GH81_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983">
        <property type="entry name" value="ENDO-1,3(4)-BETA-GLUCANASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983:SF0">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17652">
        <property type="entry name" value="Glyco_hydro81C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03639">
        <property type="entry name" value="Glyco_hydro_81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52008">
        <property type="entry name" value="GH81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 81 N-terminal" evidence="10">
        <location>
            <begin position="68"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 81 C-terminal" evidence="11">
        <location>
            <begin position="402"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="22"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4371399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="769" mass="85607" checksum="A87376F917CA0526" modified="2023-11-08" version="1">MGKPHSKPVVSPTPKNGKRQSGRNSRSGILTSFGNSKTTTIDDIPPDNIFVPIQPDIILPQIPIGAHHPVPKTGVEDDDKRTMNTNKFYANAFLGQQNQPIWTHPYSIWWGKGWQDQGLLQTWGMNIAHNEENDVNYGAGDPASNYVNQLHKQSLILSAKELDPQTTLTTDTHLPFSVNININSHSTPQEPKITFPVVQGMSFVTAGYRDATPSIQTGGKGFVDWAEAIPMGRTTKYRVKDLDGRDWVIYVNPVAGLDYDGTKFIRLDPNTLIGPPGFKGTIQIAKNPLGDAGEALYDKACGAFPYEVKVTAVAPDIKGSYSFTYTKIGSSPLLIFALPHHIQSLNPDLRKQVTGLQLRTTTKGMATALWAEQLTFVESNLPSTMSFGPWNPNSSGTAKARYAPDVLALIAANAERDLRRAMTDPIPTDSYYYAGKALAKFTTIVWVIKDVLANESLTTVGLDNLKQQMAKYIGNQQLHPLYYDDTWKGVVSNAGFTDAGADFGNTYYNDHHFHFGYFVYTSAVIGYLDPTWLSQGDNKAWTNMLVKDYAESDYNGRDYPFSRSFDWWHGHSWAKGLFESADGKDQESTSEDGFASFAVKMWGKVIGDVNMERRGNLMLAIQARSFNSYFYLQSNNTNEPAHYIGNKVAGILFENKVDYTTYAHTSAPPPSLIHGIHMVPINPASALLRRRSFVKEEWDAYFSNNRAEAADGGWRGILLANLALVDAKTSWAFFRDGVGGYWDERWIDGGASRTWYLLWAAALQEFGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNH9</accession>
    <name>A0A9W9CNH9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxypeptidase</fullName>
            <ecNumber evidence="6">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_004786</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNH9"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF404">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041015558" description="Carboxypeptidase" evidence="6">
        <location>
            <begin position="21"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="647"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="761"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="850"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="647"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="850"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="879"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="948"/>
            <end position="959"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4371567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="994" mass="107462" checksum="BCC3821261DEE79D" modified="2023-11-08" version="1">MFQLLPIALAYAVLIICSLAQQFPPPVTYDAIIPSPVDPNITISYRQPSPGTCATTFDTQKQYTGYVNLPPFTLMPYQQNYSINTFFWFFESRTNPETAPLTVWLNGGPGSSSMVGLFQELGPCEVVLLPNGSYGTQSRLWGWDRSSNLLFIDQPTQTGFSYDERVNASVDFTLDAPFGLDSRLAWQDIDAGAPSWRFMNGTFASGRKANTQNLTAIAARACWHFLQGFLSVFHQYNPGVRPNSTSVEAAGVHLFAESYGGTYGPIFADFFEDQNDQRRNGSISADTLEIRLESVGIVNGLLDPLVQTIAVANYTRNNTFGIVGIDRVTFENIISYATSGTGCRGLIAQCRSEIAIGDRESAGPNDNIDRHCKQAIKACLVAAEIAFKDASQSIYGIRVKPEVWPGDAYQEYLNSADVQISIGAQVNFTQSSTTVYNAFEDTGDAVRGTQLSSLAELLARGVRVALIYGDADIICNWYGGQNASLELARLLPGYDASFPRAGYADIVVNNSYVGGQVRQYGNLSFSRIYDAGHMVPYYQPETAFTVFTRIIQGDDIGMGRNVDLSTFGTEGLENSLHQNKVPDEPASTCWLRDASSTCTEEERAAMRQGQGIVKAGIWVHGDESAPRSTSQGHPVIIATKPATTSSIPRTGVYTATGTPIPRPSVTSGASRRVNSNFDLLRRAFTAFDPAQAERDADSARKTRNGLIGGLSAAGALLLSLLCCRCCRRRRNKDGILPVDTEKAPAVIVAPVRDAEPVASRHPTIMDPIPEMSPRPSKDTVPTLRSATVASRAPTVVPSRRPSDTVQEPTTPVEVIPVVPVQRAVTAHQEPPTRAPTMVLVPLPVQSQDFAQAPQPEAAPPATRSESVSHPAPTRAPTVAPLPPRPNPIPTPPRTPTPPPQGPEAEPEPAPEPKESPAPAPEPNDQPESDVASPKPSLITRVKDAIFDTGDEKEDAAEEAAPERDPTPPPRVATQPPLFRAPTAPLPSRHAEEQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNS8</accession>
    <name>A0A9W9CNS8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Exocyst complex component 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SEC10</name>
        <name evidence="8" type="ORF">N0V83_004142</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNS8"/>
    <dbReference type="OrthoDB" id="125856at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009976">
        <property type="entry name" value="Sec10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048627">
        <property type="entry name" value="Sec10_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048625">
        <property type="entry name" value="Sec10_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100">
        <property type="entry name" value="SEC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07393">
        <property type="entry name" value="Sec10_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20667">
        <property type="entry name" value="Sec10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec10 N-terminal" evidence="7">
        <location>
            <begin position="67"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex component Sec10-like alpha-helical bundle" evidence="6">
        <location>
            <begin position="196"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="71"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4372368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="841" mass="94325" checksum="C2F15AAA3F7E8CAC" modified="2023-11-08" version="1">MQNSNAETVSQLTHATSRTSIRGPQFTLDKFSSKDFIVKDFIEDLSDAAVPASRKSGAAAGQQAFDPKPLIRTFEHALTRLNNLSEDLEEKENELSGAVRRAEAQHNQNVESLGRRLEQAIDRFQRLDSSLNGGDDGGPDSGGNIAMRIGERLEELDRQRKRALDAKFLIECWQEVSERGELNILEDQRRSGDIVRCAEIARQLLRISIRLDPEANPRANGEGQTNAKKRLQRPKHNTKEVIEKFLEGLEQDLLSKFDECYRRPNYNGMRDCAIALRGFNDGASVITTYVNQHSFFIDRTQLLAEGLNSDPETWEHLQDPDAEPPLVETTLQSLIDEVKIVVQEESAVMKRAFPYYEEVLIRFVERIFQQSIQQRLEMVLDKASELSSLAFLRSLQASRAYITQLVEDLKTHGLTEHPEPVTSQVAATLDQQLDELFSSYFIGEKYIEREKKNLEELYSSLLLKFTIYHSRRAKMPTSYFGSLAQRGKELAASARDKYMERLESTDLPASQKATLLRIAGLKEDQQEKREIDVTDEDGRLSLSVAKRMLKWLAEGVGRGLELSPSNETPKDVQILLSLLLRQMGEIYLETALEAAQDHAASQENLKTPPDLTHLPSLHTITTILHLLQQTSTTILLPLCSPNLSIRRELEKSSVVTMATLESKLSNILNLTLTASLNWVNKCLAQQKKTDFRPKDDDLMVTSETQACHEVVAFLTRVATQATAALSGRNLSLFLAELARGLRSLVLAHLLKFTISQVGGLSVSKDMNRYVELVRGWPTGEDLERGAMDVLTDVSTLFIIGPEALRDRLRAAGGVDAQELKGFIGRRDDVNSVGVQSVLSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNZ5</accession>
    <name>A0A9W9CNZ5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribosomal protein S15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RPS15</name>
        <name evidence="5" type="ORF">N0V83_004144</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNZ5"/>
    <dbReference type="OrthoDB" id="10258210at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.860.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_uS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_uS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005713">
        <property type="entry name" value="Ribosomal_uS19_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003121">
        <property type="entry name" value="PRK04038.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01025">
        <property type="entry name" value="uS19_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="Ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4372370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="188" mass="21681" checksum="F7A45C30694C5987" modified="2023-11-08" version="1">MADSEYNAEEAAVRMSTRRFQNEPKTFYSRRTHTGIVKGPGLTIYLVELKRKRAFRKFSYRGVDLDQLLDLGSEELRDLVHARARRRFNRGLKRKPMGLIKKLRKSKQEARPNEKPDLVKTHLRDMIVVPEMIGSVIGVYSGKEFNQVEIKPEMVGHYLGEFSISYKPVKHGRPGIGATHSSRFIPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPA7</accession>
    <name>A0A9W9CPA7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-hydroxyisobutyrate dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_003298</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-oxopropanoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17681"/>
            <dbReference type="ChEBI" id="CHEBI:11805"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57700"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-valine degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HIBADH-related family. 3-hydroxyisobutyrate dehydrogenase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.31" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPA7"/>
    <dbReference type="OrthoDB" id="21615at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008442">
        <property type="term" value="F:3-hydroxyisobutyrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000056">
        <property type="entry name" value="3-hydroxyisobutyrate dehydrogenase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002204">
        <property type="entry name" value="3-OH-isobutyrate_DH-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="HIBADH-like_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015815">
        <property type="entry name" value="HIBADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22981:SF7">
        <property type="entry name" value="3-HYDROXYISOBUTYRATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22981">
        <property type="entry name" value="3-HYDROXYISOBUTYRATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000103">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00895">
        <property type="entry name" value="3_HYDROXYISOBUT_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0101">Branched-chain amino acid catabolism</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase NADP-binding" evidence="9">
        <location>
            <begin position="7"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyisobutyrate dehydrogenase-like NAD-binding" evidence="10">
        <location>
            <begin position="196"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000103-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4373007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="330" mass="35065" checksum="3D2F25B7F683F208" modified="2023-11-08" version="1">MATPDKTIAFIGLGVMGYPMALNLRKGLDKSHTILICDVSSDALERFQKDAEGHGPVEVIKNGYDAVQKADAVITMLPDSKVVKIVYLDSQTGILAGAHQAFTNASGAEKKGKLIMECGTIETSTILEVAKAVETSSVSQHLTFVDAPVSGGPMGAQDGTLAFMAGASNKEIWPTVKGYLSHMGNPSSIFLCGDIGSGTAFKVINNYLSAITSLAASEALNIGAKMGLNLELLTDVINVSGGQCWVTSKSNPVPGIQKNVPSSRDYEGGFRIELCAKVLGMGSQLAEMVGARTILDKPTLEAFGEAMHDDRYKGKDARVVYKWLNESKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPC4</accession>
    <name>A0A9W9CPC4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001796</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPC4"/>
    <dbReference type="OrthoDB" id="3923202at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="144" mass="14831" checksum="9F3FB90BD6DB0277" modified="2023-11-08" version="1">MACPVIGTTNDVLPPNHPSIDLSKDGQTCPVVGATTEHHHNLHTHPPVPMPANSTTATQCPALKSAVNDPKAQQMDDALCPVVGTATTVLPPDHPDMLKASAGDVCPVTKATVEHHKDKVVTHPSVAGTADGAVCPVTGQKHLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPJ4</accession>
    <name>A0A9W9CPJ4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Inosine monophosphate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001903</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPJ4"/>
    <dbReference type="OrthoDB" id="2349068at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018580">
        <property type="term" value="F:nitronate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04730">
        <property type="entry name" value="NPD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004136">
        <property type="entry name" value="NMO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32332">
        <property type="entry name" value="2-NITROPROPANE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32332:SF34">
        <property type="entry name" value="2-NITROPROPANE DIOXYGENASE FAMILY, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03060">
        <property type="entry name" value="NMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4374825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="36556" checksum="0E5D2655B51D9B09" modified="2023-11-08" version="1">MSTLKEQLPWTSSPIIVNAPMAGFAGGKLASAVTLAGGLGFVGGLSDMKALRKELQIVSEAFTSSPTLSTSRTTVPVGLGVLPFIQNLEDVVPVIGDFKPAAVWLFATKSLDDYAVWASAVRKVSPETNIWIQVGSVSAALYIARTARPDVLVLQGADAGGHGFEKGAGIISLLPEASDTLATDGYSHILLVAAGGIVEGRGAAAALALGAQGVVMGTGFLAAQEINIHPNYQASILEAQDGGQVTTRSKVFDELNGPNLWPKEYDGRSLIVQSHKDHVEGLGLEEIQRLHKEAVGEDDKGYKTGLQGRAAIWAGTGVGLVKKVESAKDIVEDLRRDVKEILGRVARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPL4</accession>
    <name>A0A9W9CPL4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002527</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPL4"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040848016" evidence="2">
        <location>
            <begin position="21"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="156" mass="18204" checksum="624B35B96EFDBC77" modified="2023-11-08" version="1">MGTNLVAWFLCYWLVPNTGNEELEDVFERLLVPTKFAFIYTCASLWRKITKPVDYCLRMGRKTWVESGHGRPLEAVEFEFQQFKDMCDHSEAARKSLKQSGKKGKTHIKVAWKDTWQALSARYQQDMEMTAQGNETLNQEEKELEDCQNGTLDRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPN2</accession>
    <name>A0A9W9CPN2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Acetyl-CoA transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_002587</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPN2"/>
    <dbReference type="OrthoDB" id="6415790at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008521">
        <property type="term" value="F:acetyl-CoA transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035348">
        <property type="term" value="P:acetyl-CoA transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000289">
        <property type="entry name" value="Acetyl-coenzyme A transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024371">
        <property type="entry name" value="AcetylCoA_trans_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004752">
        <property type="entry name" value="AmpG_permease/AT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778:SF9">
        <property type="entry name" value="ACETYL-COENZYME A TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 33 ACETYL-COA TRANSPORTER -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13000">
        <property type="entry name" value="Acatn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="205"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="399"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="430"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="467"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="544"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4373848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="583" mass="64292" checksum="B81F45F25D2D83F9" modified="2023-11-08" version="1">MSKRASRSKNRGSVLRQQSSTDAIREIGNGHANTSATMNGVMLEHRRRHNSIDGTSPSSQAASLMGSSSFTLDDDVPNLGKEEEAPSKGFFELSKKDQRNFLLLVLLYFLQGIPMGLAHGSVPFLLKNNVSYSAIGVFSLAAYPYSLKLLWSPIVDAVWSPKVGRRKSWILPIQTISGFSMIWLGKNINKMMKDAGDSDGAVWNFTWWWFALVFLCATQDIAVDGWALTLLSNENLAYASTAQTVGLTAGQFLSYTVFLAFNSKDFANKWFRPLSAPGETGLMELDGYLTFWGWAYLVVTFGLAIMKKEDRDKNGDSIKEVYQTMWGILKLKNIQSFIIIHLIAKIGFQANDAVTSLKLLDKGLKQEQLSLVILIDFPVEVFLGYYIGKWCQSYPPMHIWCWSFVGRLVAAGFAQFVVSIFPAGGATSGFLLLVIAEHVLSTFMNTVMFVAVSAFHAKISDPVIGGTYMTLLATVSNLGGTFPRFFVLKAVDMFTSATCQPPTTPPKGGLLKGPLVTQSFSCSMEADKHRCRDGGGICNVTTDGYYIVNILCIIIGIVTFWGYIKPAVMRIQALPLKAWRLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPU7</accession>
    <name>A0A9W9CPU7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_002781</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPU7"/>
    <dbReference type="OrthoDB" id="3437016at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17502">
        <property type="entry name" value="MFS_Azr1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000013">
        <property type="entry name" value="Related to multidrug resistance proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF84">
        <property type="entry name" value="VACUOLAR MEMBRANE AMINO ACID UPTAKE TRANSPORTER FNX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="269"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="336"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="399"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="430"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="467"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="77"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="761"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="762"/>
            <end position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4374042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="876" mass="95757" checksum="A3540362554D5831" modified="2023-11-08" version="1">MTPQIPQQEHQHTEASPLLPKAVDDAHIIDASSGIAPGTADAVVEENDGGDIERQVSHGDSIKHQGLPEVQKRMKYIFPALAIGVFLAAADQTLVVSTYGIIGTELHALSSTSWIATGYFLTLSAFQPVYGKLCDIFGRKECLLFTYLIFGIGCTFCGLARNMGELIAARAFAGIGGGGMSVCVSIMLSDIVSLRDRGTWQGYINLVYACGASAGAPLGGLLADSIGWRWAFLAQGPLCLAAFIAAALTLRLPKQDHSHWKEKIAKVDFLGAVILIVAIFGLLVGLDRGSNVSWSNPIAIAGLATTPLFIVFVLVEKYVASHPFAPGHIIFHKTLFACYLCNFFAFGGWLAALFFIPLYWQVIGDYSASRAGLLLVPSIVCGVSGSLFAGFYMKRTGKYYWITVITYTNVTIGLALVLLFAGTITKNLPLMVVGTCICAFSNGLGVTTTLIGLIANASHTDQAVATACSYLFRSLGSVFGVSMCATAFNQTLRKSLQSALHGDKDAEEIAERYVIYTVEFSCHGIPQTLRQLFNSSMGPAWRIVQPYCVGLAPQVNSTEPLWYVGCKTMSGEDKIFYSQTYFDINYAELARWSKTFPNAPRLCFVTFGQNLSYFASAPGHGSIWAGIPSELEDKVRKTFDTPCCVALGQKNAWFVLYPDGYMAWKFHGHYSALEKLLKEAAPRSVSYVAISPYNKEHYFVAFTDRSIKYNFKGAPPEWMKLMTEVFDAWAAERMQKQPQPFYPPNPAMYPPQQLYAQAQQYPPQPQPYPQMQPYPPQPQPYPMGRPQSYYGNSPPAVPMANPMSPPLTPNTPLPLYASPVPHNAVPAEVYGYRAPQGGAIEMPAELPGEVMLSAPAPVPVRSTSTDKKKKFLSRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ78</accession>
    <name>A0A9W9CQ78_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Arrestin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ78"/>
    <dbReference type="OrthoDB" id="2333384at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="51261" checksum="8F876E7637959F52" modified="2023-11-08" version="1">MAAVRADQQPSRPQPRHLYSYVAQPQLYSDHYPNRENTPEGPISIAFTKSSRRSIHTNNDLVKGVVHCKSRIHIKRVTIRFIGRTTCRIFNERNPTNQHVAESILFCHEKALSKRETPSATCPPGRVEYSFEFRFPEAVEQDPTMQNGAQFKADEFFEHERGHMLPPSLWWNESAVRSEYFLEAEFYTEQTSFTLNPVVIQQLRFSPSVPEQSLSVSIPLLSAPPIRFERKSRPSTPEPSLERRGPLKRIKSGLRGGDKAEETSSTSLIVLSAPSHYRVGAPSTLKLSLQATLSEGNCRPAPVFLRGIRAQAIAHINYRIPSSSASNGEYRKDSIEKFDLFNQRYSKPGLEIMDNTTVDGLDINTIIPPTFKTYGLAVHYDVRYDLLLDCAGKESEHEIEMKDVAVEPMTRPGGHLGPPLDPPPPRSSDESFALDELMRLGGTGGPGVEDPPAYYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQJ7</accession>
    <name>A0A9W9CQJ7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQJ7"/>
    <dbReference type="OrthoDB" id="3914029at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="249" mass="27308" checksum="EBEC27A76FBABEE4" modified="2023-11-08" version="1">MSANEKDNLPPPYSPNYTPQTVSSPSRGQNILDQLTLTRAHHIQTVIDTHIIPLVETQASYGIAQTTIAMLPSDIPLPVPEEKTEFSFETTAGDSKAVEVIGFSSQDEPKVVRLEGMMNRTEFWRPQAVIVELERVLRESLNASTRLRSPTGGAGATRVGRGSSSEFDSPAQQQLPARRNFLSRLVPSLGQEERSPSGNPEVGTKGMEGGGLVLVKVRLEEICLRTVTEFGLYDTMSKQCVIIRVDARC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQS2</accession>
    <name>A0A9W9CQS2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dityrosine transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">DTR1</name>
        <name evidence="7" type="ORF">N0V83_002258</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQS2"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005275">
        <property type="term" value="F:amine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF21">
        <property type="entry name" value="DITYROSINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="373"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="401"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="436"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="465"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="63"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4375174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="504" mass="54500" checksum="FFAF903387289B75" modified="2023-11-08" version="1">MRSPIDFEKAHHYDRCRESIDSDDYPSPHSIDEAELSLTDNAAATPPPLPPYSAFSINRRRFILAVVTAAGFFGPLCGAVYLPSLKLFQKIFSTSETAVNGTVSMYMVVFAIAPLFGAAASDIGGRKTVYMVGLGSFLIANTLLAVLPPRIVLLYFLRIFQAFGSCIVFSVGAGTVADITEPASRASALAWFLMGPQLGPILGPLIGGQFATESRWRWVFGFLSITSLPVYLAIVLCMPETLRSLVGNGTALAHQPWFSIPKFRSKPCTDSKVPKVPRPSFRKFMHLLKYPPHLIVSFNGAFQFAGLYGMYISFPTVWIDGYNFTTAQVGYAFLCPGIAMFVSSIVVGRLSDYMRKKAIANSPDGKVAPERRIAIQIPGFLIAAAGKIMFGWFTYNRVHPAAGLFAAAIAAAGVSVVFVTSTSFQTECDPAQTASLVALGGFLRNVAAAICAAIIGGILKGMGWGWAFTGLGILDLICIPGIILIMVRGARFREALKREQQLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR22</accession>
    <name>A0A9W9CR22_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="7">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PKP2</name>
        <name evidence="11" type="ORF">N0V83_000321</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR22"/>
    <dbReference type="OrthoDB" id="407390at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF25">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE (ACETYL-TRANSFERRING)] KINASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Branched-chain alpha-ketoacid dehydrogenase kinase/Pyruvate dehydrogenase kinase N-terminal" evidence="10">
        <location>
            <begin position="60"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="9">
        <location>
            <begin position="276"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="370"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4377496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="461" mass="52262" checksum="17D8971E8450CE18" modified="2023-11-08" version="1">MNTGRSSLAVRPWRRGSYPRAFPRLSLPPGQYRFAQAAAVAPAWRPSSALDEWVQREARPISLRQLSFFGRTLTESRLLESAKYCRLELPTRLAHRLRDIQTLPYVVVANPHLAHVYEAYLRAFERFRRVPEVRSLEDNDKYCKVLTETLREHATVIPRLAIGVLEVRGLMKSEEIDKFMTTMLRSRISRRVIAEQHLALTETFNSPWHFPQAERPPHDQEAVGEIFLRCNAKEIVETCGKTTQDLIKGAYGSHVNIPEIKVYGHLDATFPYILSHLEYIIGELLRNSIQAVIEQRKSKNAQLPPIEVLICETSQHVIIRISDQGGGIPNEVLPYLWSFSKGPRRDQRLENLAKVPKLLGKLQELQVPGEESAADLQHKHSKKSKQANAGAHHGSLSSLTGRPPDRRLGIGLPMARLYAEYWAGSLEIHSLEGYGVDAFLQISKLGNKNERLTTRASMDAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRF5</accession>
    <name>A0A9W9CRF5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000701</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRF5"/>
    <dbReference type="OrthoDB" id="3202396at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025444">
        <property type="entry name" value="Monooxy_af470"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13826">
        <property type="entry name" value="Monooxy_af470-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="206" mass="23094" checksum="4C580FEC49C05C5A" modified="2023-11-08" version="1">MKGVIPYRTSVVLPNEKGIMSDNPAESPVCAIVLAVISNHPLGMFAEGFKEIGDRFDDMVREMSADATKHGFLGSSTWINAGERTTANEYMSILYFENEHALHEYAHGPMHTATTQWWRDTEKRLKHIGIMHEVFACPKNSWEAIYLNYKPVGLGATTKEVTSETGQKEWVNPLVKIKGKLNYSKGRMGRSVGAKEWDTLSTQVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSJ4</accession>
    <name>A0A9W9CSJ4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSJ4"/>
    <dbReference type="OrthoDB" id="843225at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47815">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN A FAMILY PROTEIN C25B2.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47815:SF1">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN A FAMILY PROTEIN C25B2.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="21"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="186"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4378172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="239" mass="25814" checksum="66669CB26EA2A8F3" modified="2023-11-08" version="1">MTCLQNIFRQLLTVWQINLYEPAILVVGTRGKSLGGFQGLLPGSVSKYCLQHSPVPVIVVRPTSKRDKARSKRANDPDRQGYRDILAKSESLLDVNSPRNSFFANEEEASAAATSGPKSTVDLHPLAQVAHANDSDDDRDTGTSTLIDDMRSPRSPAVLMKSPHLQNLDSPELSSADSSSDEEEQGGVSTSRVVSGVEDLSPGSEEPKSKTGEPTSYVDKLAKAATTEEPQHEPVQKDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY86</accession>
    <name>A0A9W8XY86_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitochondrial distribution and morphology protein 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Mitochondrial inheritance component MDM10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">MDM10</name>
        <name evidence="7" type="ORF">N0V83_010652</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the ERMES/MDM complex, which serves as a molecular tether to connect the endoplasmic reticulum and mitochondria. Components of this complex are involved in the control of mitochondrial shape and protein biogenesis and may function in phospholipid exchange. MDM10 is involved in the late assembly steps of the general translocase of the mitochondrial outer membrane (TOM complex). Functions in the TOM40-specific route of the assembly of outer membrane beta-barrel proteins, including the association of TOM40 with the receptor TOM22 and small TOM proteins. Can associate with the SAM(core) complex as well as the MDM12-MMM1 complex, both involved in late steps of the major beta-barrel assembly pathway, that is responsible for biogenesis of all outer membrane beta-barrel proteins. May act as a switch that shuttles between both complexes and channels precursor proteins into the TOM40-specific pathway. Plays a role in mitochondrial morphology and in the inheritance of mitochondria.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the ER-mitochondria encounter structure (ERMES) or MDM complex, composed of MMM1, MDM10, MDM12 and MDM34. Associates with the mitochondrial outer membrane sorting assembly machinery SAM(core) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Mitochondrion outer membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="6">The ERMES/MDM complex localizes to a few discrete foci (around 10 per single cell), that represent mitochondria-endoplasmic reticulum junctions. These foci are often found next to mtDNA nucleoids.</text>
    </comment>
    <comment type="domain">
        <text evidence="6">Lacks alpha-helical transmembrane segments, suggesting that it resides in the membrane via beta-sheet conformations similar to those predicted for other outer membrane proteins and porin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MDM10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY86"/>
    <dbReference type="OrthoDB" id="2103793at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001401">
        <property type="term" value="C:SAM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051654">
        <property type="term" value="P:establishment of mitochondrion localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070096">
        <property type="term" value="P:mitochondrial outer membrane translocase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03102">
        <property type="entry name" value="Mdm10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027539">
        <property type="entry name" value="Mdm10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28035">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28035:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12519">
        <property type="entry name" value="MDM10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1 6" id="KW-1134">Transmembrane beta strand</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4362558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="392" mass="44394" checksum="A70FE12A2D18DC2A" modified="2023-11-08" version="1">MLDFMENVQNAFYEASHWNVDNSYGALNATARSLLEFQIPRGLRLQISSLAAPNFATSYTLGSVGVVDGSVSYLYSSLPLRKEFRSSHIDLHHVIRGYKHLQELRRPDENWWWEVWHAGKRVDRKNTLLYGRIFLPQSRLEALYLRRLTPNRQLRIAAVSDSNLNNGGTILTLLQNDFGKYSTEYMYSTDSALMGVRGLYNFGPDPRVAPTEPVAAGEVEPVHGRFSAGAELYYGLLNKSGGISTGVRFTTLPNHPGFPYTMTLTLNPLMGNLSSTYAVKAGPNLALCSRFDFNFYSYESELQLGCELWRRRNTADTEWAVKKLRPDWKQPSVSPDDDVSGVLKATVDQDWRIGVLWEGRIKELLFTLGASLDLKKREQIFRSVGIELQYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYH4</accession>
    <name>A0A9W8XYH4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_010239</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYH4"/>
    <dbReference type="OrthoDB" id="2985014at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000065">
        <property type="entry name" value="Putative MFS pantothenate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF32">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="409"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="41"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4363119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="487" mass="54554" checksum="D3FEE0C66261F812" modified="2023-11-08" version="1">MATFEEKGVFAPATQSDVSDTFDLEKDAGALRARRKVDTTVLPLLFLGLLVFQLDRMNLASALTDGFAKDIGVNQNTINLGNQLMFLGIVVLEIPSNIILQRIGPRKWISAQVFIFGLVATLQVFIKNRTGFLVSRTFLGLCEAGYIPGGIYTLSTWYTKRELAKRVAVFFFGMFGGNAISPLLASGILKLRGHGGLTGWKWLFLLEGIFTMCVSITLLFLLPGSPDHPRPLLSPGLIRFKDSDREALQLRLHADDDTRTAENKGLHIPLSLVWKTVKHYRRWPHFISTFAVFSTWSSLTTYTPTIITALGFDRIEANALACVGGFLALFVVFFFGWLSDKTNKRGLSVIIAQFLFLLALIITRAVHPHVGKWSRWGLWTTVNAFAIGYHPVHNSWVQLNCKDPRERSISIAMWVMSAISGLMAGTQYYRADDTASFYQKGLRTQTILVAVGMVFAIVQVGVYVLYNKKVLRRWKTEGGEKPWLYTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYS0</accession>
    <name>A0A9W8XYS0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYS0"/>
    <dbReference type="OrthoDB" id="10538246at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="255" mass="28620" checksum="7E305294D3C9ED77" modified="2023-11-08" version="1">MATYSPTVASLFDRLYRAHLQAEGPPAGRTRSQTGARAATKTPGLNPGSTKVTKPRRRNQPAAARRLCAKRLQLKKRLERDEEYRDDSGVHLTTTDIRRRVRQLSCEARSGTLDQLPRPHPRTVRSLEDIAAARRKKLAGPSSLPTMERALSPEDEIEEWGLLPGEVRDDEPDFEMEQDLLDLLMVDEENLEALADGEQAVGVWRRLLAAGSGSDTERDMVRRAGEEAAWRRRAQEGAVMDGRCVAIVKDVWRFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ73</accession>
    <name>A0A9W8XZ73_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="9">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_010090</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ73"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000037">
        <property type="entry name" value="alpha-1,2-Mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF29">
        <property type="entry name" value="ALPHA-1,2-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="340"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="458"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="520"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="627"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="415"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4362973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="645" mass="73937" checksum="FC0533C2166D5702" modified="2023-11-08" version="1">MRRIKVTRGVAYQPMSRQHKRTLTYLLLGAFAFSVYYLWSARPIDPLAVPFHVHSTYMSDRGATGKVVSRSSYDWRRAPFVNKIEPSILLPTGEPRNLPPVQYKDFAETRSQKQQREARRVAVRNEFQHTWENYRKFAWVQDELKPVTGQYEETFGGWGATLVDSLDTLWIMGFKEYFWEAVEAVAAIDFGKTDLKTISVFETTIRYLGGLLSAYDMSHEPVLLEKAIQLGEMLYRAFDTTNNTPLGWLDIENTKLVDREEFNAETSICFACLGSLTMEFTRLAQISAEPKYYDAVARVTALMDRVQNTTKIPGLFPTMANAAKEEFNQSHAFSIGALADSTYEYFPKMHALLGGLEPVYEKLYTDSAAMIDKHMLFRPMVPYDPIGESLLLCGDLFTPRPEDITLDATMQHLTCFIGGMFGLAGRLFSNSYHVDVGAKLTKGCIYAYATTPTGIMPELFDMVPCASRTSCPWNFTLWEENVMRHYGGKYIPDVEAAMREYHIPLGFTNIRDPRYLLRPEAIESVFIMHRITGDQQYLDDAWDMFVSIVEASETPYANGQVLDVTFRPPPPGRERQRTPGVRGGIYTREENIEDKMESFWTAETLKYFYLIFSPPDMMSLDDWVFNTEAHPFRRPHNVKKQKGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZR2</accession>
    <name>A0A9W8XZR2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010503</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZR2"/>
    <dbReference type="OrthoDB" id="4590707at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="244" mass="26456" checksum="F1BED66A92CBF89A" modified="2023-11-08" version="1">MSFIRSSALRASSLARAARPTRYIRTAELQPWQRAVQRRTYASAHGAHGEAKSSDVPWAAAAAAGTAVGLYMVLTQDLSHGESHDDHHGDAHGKEHADEDAPADEAKDEEPKEESDDDADAKTDSKDEKKDDKKDDSEDGKKDKSQDNKVAQPKKEGNKEASPDKSDKPDPNKASKSQNEMSGKQQGLSNDDTGHSSQIDQQPEKSKKGEGVAESAKLKGTVSTDRPQAENKEERGKAQQDKDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y057</accession>
    <name>A0A9W8Y057_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">glucan 1,3-beta-glucosidase</fullName>
            <ecNumber evidence="9">3.2.1.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_009622</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1-&gt;3)-beta-D-glucans, releasing alpha-glucose.</text>
            <dbReference type="EC" id="3.2.1.58"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.58" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y057"/>
    <dbReference type="OrthoDB" id="62120at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000033">
        <property type="entry name" value="Glucan 1,3-beta-glucosidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF1">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE I_II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040745084" description="glucan 1,3-beta-glucosidase" evidence="11">
        <location>
            <begin position="21"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="12">
        <location>
            <begin position="81"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4364167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="408" mass="44374" checksum="55A90E2F73D9D69A" modified="2023-11-08" version="1">MVGHWTTAAAVSLLALSTNASPLQPIKRSPPFDYNGQKVRGVNTGGWFVLEPWITPSIFEGNAAVDEWTYTSIMGKDAAKSALQNHWDTWITQDDFNQMAAAGLNHVRIPIGYWSVIPRDGDPYVQGAYDKLGEALDWASGAGLKVMIDLHGAPESQNGFDNSGKYGSISWGTSDSVTYTKNVLNKIRDDYASHPAVSAIQLLNEPLGPDLDMGTVKQFYMDGWGNLKDSNVAIAFHDAFQGVTSWGDWGAGMWNLLLDTHHYEIFDNSAVSQSIDTHISTACDFGNQMASTGKWTIAGEWTGGITDCAKWLNGKDKGARYDGTLSGSSKIGDCTGKSTGSVAALSDADKSNIGRFIEAQLDAFEKATGWIFWTWKTEGAPEWDMKDLLANGIFPQPLTSRKYPGQCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y099</accession>
    <name>A0A9W8Y099_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Altered inheritance of mitochondria protein 41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 2" type="primary">AIM41</name>
        <name evidence="2" type="ORF">N0V83_010197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIM41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y099"/>
    <dbReference type="OrthoDB" id="538640at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016884">
        <property type="term" value="F:carbon-nitrogen ligase activity, with glutamine as amido-N-donor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1510.10">
        <property type="entry name" value="Uncharacterised protein YqeY/AIM41 PF09424, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019004">
        <property type="entry name" value="YqeY/Aim41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042184">
        <property type="entry name" value="YqeY/Aim41_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28055">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 41, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28055:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 41, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09424">
        <property type="entry name" value="YqeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="GatB/YqeY motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="186" mass="20358" checksum="EE7D23B3B7D1E4AA" modified="2023-11-08" version="1">MSLFRPNLLRVGLPSASRPLCLRTAVRFSSTSTSEAIILPRLQSDLKNAMRAKNKPALNTIRALQAEIINASKTAKPITTDGAFYSLVQKQIKASNTAIGEFQAAKRDDLVAKEQEQLDVLQKYADEIPKVAESEIDDMIEKVVAGLEEGKRSFGAVMGKVMGAISGRPHDVEYVTKKIQETVGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0G2</accession>
    <name>A0A9W8Y0G2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0G2"/>
    <dbReference type="OrthoDB" id="66144at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF110">
        <property type="entry name" value="RHODANESE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="71"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="215" mass="23193" checksum="D4EC35F99CA08CEC" modified="2023-11-08" version="1">MASTNKQSLLDRVFAAKSLTESRALYDEWASTYDSDMTLHDFTAPRMVAEAVARGLKLNHISDKPLTDLKIVDAGCGTGLVGIELAKLGAKDIVGLDISEGMLNVAQKIGVYGDLKVTDLTKRLDANDGTYDALTCCGTFTHGHLGPEPLGEFVRVVKTGGVVVATVLDSHWMEKGFESEIGRLAEEGKVEVVEKDSHDYRKDAGGGRVLVLRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y130</accession>
    <name>A0A9W8Y130_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Protein transport protein BOS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">BOS1</name>
        <name evidence="16" type="ORF">N0V83_009026</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">SNARE required for protein transport between the ER and the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 12">Belongs to the BOS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y130"/>
    <dbReference type="OrthoDB" id="158360at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15863">
        <property type="entry name" value="SNARE_GS27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000054">
        <property type="entry name" value="Protein transport protein BOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027027">
        <property type="entry name" value="GOSR2/Membrin/Bos1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230:SF1">
        <property type="entry name" value="GOLGI SNAP RECEPTOR COMPLEX MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230">
        <property type="entry name" value="VESICLE TRANSPORT V-SNARE PROTEIN VTI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028865">
        <property type="entry name" value="Membrin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="126"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="63"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR028865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4364432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="254" mass="28947" checksum="701E283BC1BE80C6" modified="2023-11-08" version="1">MNALFNSALRQSTSVRKDLDQFADSASPSPHLQAQLNASLTSLSRTIDEYGKLAKQEPVQTKQEKAFERLKNFRSELEEYRESFKRIKSVNEEIQTTEARTELLGRRPHHAATPENPYAQPNLAAAAQHSAFAPSQPYDPSAPYRPNPYSAGAPQGPGYDREGHVLRENTFFNKTSEQLDEFLDRGRAVLGDLGQQREMLKGTQRRLYSVANTLGISGDTIRMVERRAKQDKWIFWGGVIVFFLFCWLVIHYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1B8</accession>
    <name>A0A9W8Y1B8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_010108</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1B8"/>
    <dbReference type="OrthoDB" id="5278208at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF10">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="173"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="684"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="120"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="179"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="709"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4362991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="723" mass="80516" checksum="8B1475A99A7EBCB4" modified="2023-11-08" version="1">MSGYYPPGHNVPPQYYNVDPNAIQNPNSQHAPPPMIPHGSYAPALPDNPFHPGAPGPFVQYNDPLIQPNYVDQYEDMSGPIGSAQSGNARARRRPAPGCRKGNRECIYPDTQSTQKSTRSGSKAGKSSSVESASSPEDHDDDRKDRLPAIFDEDEEEDEDYDVDDDCLARSQELHDSSHTPGSTLDASTSPSTEASSVVPPTGRPSLSRKGSAQATKAGPATKGSSAMARDLQFYLNYFQNHITIHHYSLKRDTHNFLKGEFLARAMKFEPLKYAVAGYAAFFHTLSQPDGRISTFLQFYNESVSRLRVSITKNKKQGLATFLTILQLAAIEEMLGDWVNLMGHQKAAFEMLTRLYTPQSITQSNFLLKVILWYIRFDLFVGFQSGGEAVLGREWYEAVHEYYAQKAREDPQDLDMKYEERFAYSRLVAKESNDLFARTGKGLVTPQEFMMQLPKLKEKVESLDKSLPPILKDPSQKVKTIAGEPDPNDIVNPYEPDVIWGDALWTSNYLCLDMWGIVFMYNVSASMALRQPFDPDLTKTAYKAVQVFEAMIAYPDAPPGTIVEAQVCFAIASLFLPKDPKTVQFVRRWFAKIEASGYIYSDVLRNRMLESMGAGPSDWWLPNDEGCPPIIRSIKDFIKDRTTAPKDQVSDDLREMRGIFSSMTISDSPPSDNVTITSGGDLGTVGVPGTEMYGSPDWASDYSSERKSSNAGAYGTSSQYPTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1K3</accession>
    <name>A0A9W8Y1K3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nucleolar protein 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 6">Pumilio domain-containing protein NOP9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NOP9</name>
        <name evidence="8" type="ORF">N0V83_008165</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">RNA-binding nucleolar protein required for pre-rRNA processing. Involved in production of 18S rRNA and assembly of small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1K3"/>
    <dbReference type="OrthoDB" id="392571at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040000">
        <property type="entry name" value="NOP9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22493">
        <property type="entry name" value="PUF_NOP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="632"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="10"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="633"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="678"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4365546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="688" mass="77420" checksum="A3E8BCE3ED3FF5E0" modified="2023-11-08" version="1">MPKEHKKRGRREEQKKRKRDVADDSSSKRHKKEEAEQAEEADVFLQEEGADGVTPGALPFYGMLDEDEQEYFKRADEMLELNQFDDLEARNLFLESVYKEADGKELKIANSQSCSRLLERLILLSTPDQLKALFQKFNGHFLNLVQHRFASHCCEALFIHAAPVVTQELTKAEPLQTPPSSDPDHVYVSMENLFLYTINELEEHLGFLMTDRFASHVLRVLLVILSGAPLERQNKSVVQSKRKEKVGISGSEKGQEWVLEKRTVPQSFVEALEKVINDSVSGFEPQHLRSLSTHPVGNPILQLLLKLELSHFGKSRAKNEKSIIHRLLPDDPIAEGTESASFINGLVYDPIGSRLLETIVENAPGKLFKSIYGEFFKERMGSLARNEIAGYVAGKILERLGKDDLEEAMRQIVDQIPNLVERNRTAIIKTLIERCVAREVDTSPIKAQLEIAYGGSNGFEVTRMLKLSEEDGKPNGKQDHSPEKVHGSLLAQTMMAADGPLGNLIFDSLAKLSPELSIQIARDPTASRTLQAALTSNNASVIFRRKVIQQFYGKVGELALDPSASRVIDAVWDGTHGLAFIRERVAEELAENENSLRESFVGRAVWRNWKMDLYKRKRHDWVKQSRYAAGNDGFQSFPESNGDSTSPQGHNNHRAGKQMTAIELARQKHAASKAAAVKRKDAQEKKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1Z8</accession>
    <name>A0A9W8Y1Z8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_008724</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>myo-inositol(out) + H(+)(out) = myo-inositol(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:60364"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1Z8"/>
    <dbReference type="OrthoDB" id="6339427at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005366">
        <property type="term" value="F:myo-inositol:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904679">
        <property type="term" value="P:myo-inositol import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000073">
        <property type="entry name" value="MFS myo-inositol transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="425"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="462"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="496"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="53"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4365106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="549" mass="59031" checksum="EE7D04A61CC9F04C" modified="2023-11-08" version="1">MTDTAAEPLIANHHRDLPDDARDHRYDHHDEEEVDQEVDESALVSPGPFIWGLTICAGVSGLLFGYDTGVISSTLVSIGSDLSSRPLSTFDKSLITSCTSFFALLASPLTGILADQVGRKVVILSADVLFVLGALLQSWTSTVGGMIAGRSIVGVAVGSASFVVPLYIGELSPGKFRGRLVTVSSLFITGGQVVAYIIGWLFSERAGGWRWMVGIGAVPAAVQFFMLFLFLPETPRYLVKAGRKAKAKGVLQKVYGEGVHGGLVEAVLRRVEREMLEEEDAAEGRGSPGVAKSGWKAKVEKVQDNYAQLVIVGGNRRALIIACMLQGFQQLCGFNSLMYFSATIFSLVGFRSPTLTSLSIALTNFAFTLVAFAYIDRIGRRRILLWSIPIMVAGLALCAVAFGFVTLPDEEHAAAASVGTSSSRTWPLVILLAMTTYVAGYAIGLGNVPWQQSELFPLSVRSLGSALATATNWGSNTLIGITFLPMMELFTPVGTFALYAAVCVGAWVSVWRIYPETAGLSLEDVGGVLRDGFGVEESVRRFRGRRRGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y238</accession>
    <name>A0A9W8Y238_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ammonium transporter AmtB-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_008787</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y238"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000013">
        <property type="entry name" value="Rh-like protein/ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF9">
        <property type="entry name" value="SIMILAR TO AMMONIUM PERMEASE, BUT NOT NORMALLY ACTIVE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="7">
        <location>
            <begin position="21"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4365169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="454" mass="49434" checksum="28EF332844D2D51A" modified="2023-11-08" version="1">MGLPTDVLKAWEACDRTDTLFVLVCSVFCWGIIPAVGIAYSGYTTRSNSLAAVMPAVLAVIVCSIQWFLIGYTLTYGEGGAIFGDFTYAFHMNVLAEPVGTIPAVLFSFFQLVFQATVCAIAVGGACERGRLLPLIPFIFLWSTFVYNPLAHMVWGGGFLMDLGVVDFAGGTPVHICSGATATAMSVYLSYPLFRSRKSSIRTPSHLVIHRPVNSLCQLLAMVSIWGSWLAFDAGTTLAFNFKSVMALCVTNLCAASGALTWMLYTYIEVGRWSLDSCFMGAISGLIMITPSAGFIDTPTAFFFGILGALVCRQALRIKFTDFARRWRWVDHGDTFATHCLGGILATIATGCFARKEVAGYDGVTEISGGVFFDGNVRQLGVQIVEALVGFFWSFIGSYAIYALIDCVTGFEVLAEDKDIIAGLDASQMDEETLWTETQKYYPYADREGALHLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2H4</accession>
    <name>A0A9W8Y2H4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008517</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2H4"/>
    <dbReference type="OrthoDB" id="5374070at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="501"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="532" mass="59985" checksum="D7DB141A075C0FBA" modified="2023-11-08" version="1">MAETHKWRRLSDRLFDIREGLDVAELTLDSWQRKYDIQARRPILYMQVLFGKQGTERLQITLGSINMTARAIKADINKVIGQALKVRSVKIPPEEMHSTVNEELVKDCLRRIRQNMSWSHKFHYSVLGKMDDLEGRLNRLHMKLTSLERLSDLYLEKEHPDIFSEVKRLPGRRVILKVGDSRMSSVQGKLLDALAARKDAELLHRASGQGNKVHIGLSVPHIHKRDFAFLLNLHGRTHEVLVHPVKIKAINDASRVQNDFPTAVPALIKNTDLACYMLPSSSTTEGFQVSIPPVNLLSDLEYKDTLSTIIRDQHAFLGSQILYSSDQSAIASGIAQGCFRLIGSQWLQFLDCGNIRWRRTKEGKWISMLTATPGNASITRTLEQCLKTNGQRRDMRDLSKHLHIFRIGLVIAELALKSPISYIDYDGVANTTKLFVNDGEEIDANDVAAEVERKSNVFLGNMAFFCLNVLQDRDAMADKSIEGSYFKEVLRNAEQLDALVKADRRRGGTSPSGSGVNTPRRGRGERGSGSVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2K2</accession>
    <name>A0A9W8Y2K2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dynamin family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_008589</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4364973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2K2"/>
    <dbReference type="OrthoDB" id="415706at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001425">
        <property type="entry name" value="Dynamin GTPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF21">
        <property type="entry name" value="DYNAMIN RELATED PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="5">
        <location>
            <begin position="45"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="4">
        <location>
            <begin position="640"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="426"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="447"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4364973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="725" mass="81333" checksum="62B10A00CFFFE45A" modified="2023-11-08" version="1">MDSPQEEWVTLAQRKASGLIRLQMSGSSRRLNQIDQIRAKGVGDHISLPQLVVCGDQSAGKSSVLEGITGVPFPRQDGVCTRFATEIILRHCPDKNEIIAKILPSAARTEEERTKMTSFERQLTGFDDLPRVIQEASVLMGIRDCVDPDLDSPAFTADVLRLEVIGNTGLHLTVVDLPGLISVSENVEDMQTVEHLVDGYLESTRTIILAVVPASNDIDTQGIIQRARRFDKAGVRTVGIITKPDLINKGTESRIASLAKNLDRVKLKLGFFLLKNPSPLDISKGLSWEGRQQQETEFFNSSPWKEQNLDKTRVGIDNLRNFLQQLLDNHIEHELPKVRKEVESLLQTTMHELAQIGPERSSISQIRMFLTHTSMEFFNLTKAAVDGNYGGRDSVFFAAGSRTDTRLRARIHIANENFAAYMRTQAAKRQMRNEDEADSGSGQSESSESEESDDTVSNEQLRLTKKEMMDWVKKVCRSISTAAAASFPEPTTTSYSELFHEQCSRWGDIARDHLLTVSSIVDKFVRAALDHVVVDDQVRSGVKRRVRQALDASLEKARDELRSILSDEDAQPITYNHYYTDNIQNARADAARRSLQVSMDHAIANEWNGKLHVSNTQVDLKKLCSSLKNRVVVDMTEQACEESLAALNAYYKVATKTFVDNVCRQVIERHLISNLVTAFDPVCVGKLSDDELHQIAAESQYVCTRRNDLQAMKKAFEESIQELRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-04-02" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2N9</accession>
    <name>A0A9W8Y2N9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009427</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3870679at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="623"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="677"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="821"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="988"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1110"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1227"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="315"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="436"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="462"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="529"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="628"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="677"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="704"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="746"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="868"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="898"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1157"/>
            <end position="1170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1346" mass="147706" checksum="C48D43CBBF5A72DC" modified="2023-11-08" version="1">MKRCGRRPITSEHLPNPQVRSTLDTGRAVSSCSSPAVAPKPPSAPGLARPARSDIGLRKRPAPFDAPTHPRTQPPREIALAAPDDCAVEPVATPTAPQSIANRAFPSRAVIQRLHGREARLPTCVPASPPGALPRPLQTTRQGREREPPVSTAHEAGHCHTALPGDGVLIKPEVEPVPQHQDWQRSEAKRGEATASAAIDAAQLANMATPQTDSYMLLEAAFHTSPNLRHHKQLPRRSDEHRSFARAAAPLNRDASYTNSEAPSVASSPAPTPLASPGAVNGAENGLPPTPPTISQDGQATEDAGSSPHADGLVSSLMSKKSSLSTPVNARSPPTPDPSPPRSTESNASNPVLERPSLFAYPSSRAESFKTAREDPMSSEPSDGRSLTPVADRLSTVDEDRGLGLAFEREDSDITPTQRTLPFYADFQQDDTTAGAEEHKASPVAEHIPDREWNTELMRNVTIRRKRTPKSSPRKARDPAVTVVESASPTPSARARRSSGLRERVEASSNSPHTPSIEDFAQSIGWPTGDKETPGEKQRDVSNKRLSTSSAGSAVVEAQIIVTPPRRQQTLRHSSKNIAYRRESTSPADLGSVTFSNRNSVQSDDVPLHRLVHKRVSVADRKKRISSESDTLGSLRTFSSPVSSVRSRTIDSSAHTLRHQESIRNVLQPAADILSRSNSLQRPYPSTSSFQRRIASAPEPAKRLQQSSGPRNFSQLSPPESPRQTENSFESSALVRDSPSALPPKPFRESPASRKEGQQRRMHPDVRHAVNINKSLPDLPKQNTDGHALQDVFVDASEPLEDTEEKRPPSALMDRVRQLVAEREAEEEAPLPITYGETPASPEPLQDMLPFQDNSPPVRRGPRSSRGRSGERRGSSHSQDRPSLSPDTLIRPSLARVSTEEMVRRSHEWRRPSEEHGRVSFDRSTIRTEEHAMARHLYAQTTPFSQLSDTPIEVSEATAVSIFPHNNHSLLVVQQVSRGSSMLPDQNQLTGGAYLTPQDQRDLQRTPTPPFVDASEEHIDSNEHPLQPTLTFEPSTPPMQIALPQPSAVDSPLKNPRPPPEPPKINFIPPTPAEELERQLVPGPPKRSDSHPQRRLTLVQRARRYSDNIIRPVLARASSNRSRHASDSHTRRHARVPTVNDEDGTLHPFWRPRGFWDDFEDSESESDEEGTLPTGGDTSDVPEPAIAPPKRTNTLGKRLTSGFKGSGGFLIGNSLGVERHGTNKRRHHVTLPPHFPKSPRTSTHASSPKILIQPPTMPLGRVPGGGISKRSSRNSLRSNAPYDVPSRRTSWRQGRSLPGLKRYQVQYIGLSGVKDRLRERTAEKRREKLRQSIGTRYYVEPGVPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2Q9</accession>
    <name>A0A9W8Y2Q9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mitochondrial transcription factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_008490</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial transcription factor that confers selective promoter recognition on the core subunit of the yeast mitochondrial RNA polymerase. Interacts with DNA in a non-specific manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2Q9"/>
    <dbReference type="OrthoDB" id="16079at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034245">
        <property type="term" value="C:mitochondrial DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034246">
        <property type="term" value="F:mitochondrial transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006391">
        <property type="term" value="P:transcription initiation at mitochondrial promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="entry name" value="Ribosomal RNA adenine dimethylase-like, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF17">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="382"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="387"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4365868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="645" mass="73525" checksum="E79591A58703803C" modified="2023-11-08" version="1">MFRVRPSISRLSRRTSASGGPLSQTRLATIYHGRSTGRQKGSKEYWTAEKLQTSDKYPLSVAISELMHRSLETKKIKKSVWSGAQIRPQIKHKGCDILDINPGAGLWSQKLHHLLQPRSHILLEPRHDKFKDYLDPMLTALGSTYSLVAKDPCDLATYRAMVTDGVFPHQRTRARLDPDAQEPNNTLLVTGTLAWDPRMPGLGFDSMAKQLFHHFSAAASTNDLFHAFGLVRTLFWVEQDDFTPMIAQSMNGMQKANRFLEMTQDVSMVVTGSLSYRKVGRGSTGREPQYEIESTLRALKAGREKHMEPPQHRRANIYDFAEDIDKLTNGTGVSRSEALLGYLREQQLAGKSTTGLLMESHIEFYNQERLLRTERPELGVIDEAASEPEKNKQRAEANSDIKGHSAENEVKNFAQKRANIRHNIKVKSTVEEIADIGEGMYKLECRISEMDDGPEKDAAMKKLEDMDRTWQRRFDNLNANYSSAPISELDDRLAIRTPPYPRLQWDRRPFLPLVMSSDEVWPPNQLLLISAEPIPHPSAQDSDGVEWVQDFLFGLYSQPTMSIEHALDKMQHGLSSIIKDCPTIKDPKKGGRLLMHHFRVRMLTIEMIEELVSAYKEWPFKSPGSDHNKYFRHKANLRSSTSSRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2T0</accession>
    <name>A0A9W8Y2T0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008285</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2T0"/>
    <dbReference type="OrthoDB" id="4664297at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4365665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="332" mass="37544" checksum="F5A2519265DF6212" modified="2023-11-08" version="1">MADAEEQFRLSQEQIDHFLEHGWIKLENCFTREQAEGLQSTLWTRLGMDPNDMSTWHTERTNMPFFNEFLVSEFAPKAWGGICQLLGGSSRVDSNASTWKDGFIVNLGTPAGHNTVIKPQKLPGWHVDGDFFVHYLDSPEQGLLVIPLWTDIVAGGGGTYICPRAIDVVAKHLYENPEGVSPRMTPRAKNPQFKQEQGLKWFNELAASMPDEAFVEATGVVGDVYLLHPLMLHSASNNQLRKLRVITNPPVSVKEPFVFDRDDGDYSAVERKTLKALGKERLDGWKIQGERQGIVPERVRIQEEMKRKEEERLARLKEGAGLEKSQEVKTMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y302</accession>
    <name>A0A9W8Y302_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Anucleate primary sterigmata protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_008375</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y302"/>
    <dbReference type="OrthoDB" id="10255000at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012943">
        <property type="entry name" value="Cnn_1N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024545">
        <property type="entry name" value="Mto1-like_Mto2p-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159">
        <property type="entry name" value="CENTROSOMAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159:SF31">
        <property type="entry name" value="CENTROSOME-ASSOCIATED PROTEIN CEP250 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07989">
        <property type="entry name" value="Cnn_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12808">
        <property type="entry name" value="Mto2_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Centrosomin N-terminal motif 1" evidence="5">
        <location>
            <begin position="105"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Mto1-like Mto2p-binding" evidence="6">
        <location>
            <begin position="1078"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="177"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="336"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="428"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1123"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="266"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="431"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1123"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1139"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1166"/>
            <end position="1175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4365755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1175" mass="135194" checksum="A3E1EF4B0258F068" modified="2023-11-08" version="1">MSGYSDSNPWEKDRSISANSISGYLQPDGPLGRLDEEVLNNTTPPETPRAPSVQPSAPTDTVIARHVQNIQVPDTIAREFRAKHSRSPDKRHMATPFTRSKHNLTLKEQNSKIDKLSKENFDLKLKIHFLDQALQNRSDEGVKDMISKNVQLQTDLATEKKDNLSLRKKLKELERRLNAQEEGVATTKDVGSGSEDEKSDQSSRQVELEEEIEFLRERLETTETIVEQWQQEALQKEADNRRMADYIKTMREKSPSGESAGIDEAIDMWKEDFEDERARREQVETRCQQAETETERLREELLRLKEQNQQMQQHATTNHHINKVYNSTRRIHQSFTTRSNSGSDGNEHVAPLSVGGTSSTLVDQLHNDNEKLQRDLHAQASMLTSRNRENLRLREENEGLKLTIRRGDVGSVAGDSILERSISRNHMRSVSRASGATRVTQMSDPERDDLESKHAALRDELSQLKLSYKELDDQLNGHLDMLETAENKVKELEKEIQAQTEDLQALANERDEALEILQDKEQECEELRQEALDTVRRLESELEQNNQERDRIFIDLENTTEDFNALQQEMKNVSESLVQLEDDRDASLRRIQNLESELADANHELGRQDKLLNDERSKNERLDIQLESCQGEIDFLREEQEGDKIKIGELESAINNAQVTIQDEKERFRDLEERITEERQQRDALDTKGKQEVEKIIADLNVQLTKLKDDNRKLRKNLSSKEVEATTWKQRLDDLEASLRESLGNLNGTRSSLLKDITKLQRDLESTVRELDITKNDLTEKDRLLRNRDALLENTGMESRRLADLLERERQARRHDQSTFENAKRGHQSVTRTIQQHETKVLELETLRSQDRQRIHGLEKQYKEQLLERNNLLYALWNRLSTLCGAEWCRNHALVNGELTSMELISKNIQGFNKNMILAIKTVESVVGGFKQRLRTMEKDLVRDYQTLEHTLDVRVKRLDQLEKLVMAQRQSIGRPSTVRGGLVDINSTEVNKLRTENKNLRTEVQTLRAITTTTQSGNEVVLSRTPSRADSLSSPKRASMAQTLLRAHSASVVEHLQNQSPGHPYPASVPLQPSEQKWIHRLKELERRLKAEREARLLDRSGARKRLEQKVEENADLRAMLEKEREKRDDDGTASIQRSVSASGSRIGRSGRASAVGSVRNGNGSDKDREEDMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3F0</accession>
    <name>A0A9W8Y3F0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Carboxymethylenebutenolidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">DLH1</name>
        <name evidence="12" type="ORF">N0V83_007759</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RecA family. DMC1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3F0"/>
    <dbReference type="OrthoDB" id="10251254at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070192">
        <property type="term" value="P:chromosome organization involved in meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000730">
        <property type="term" value="P:DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042148">
        <property type="term" value="P:DNA strand invasion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000709">
        <property type="term" value="P:meiotic joint molecule formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19514">
        <property type="entry name" value="DMC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000239">
        <property type="entry name" value="Meiotic recombination protein DMC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011940">
        <property type="entry name" value="Dmc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016467">
        <property type="entry name" value="DNA_recomb/repair_RecA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003301">
        <property type="entry name" value="PRK04301.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02238">
        <property type="entry name" value="recomb_DMC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942:SF30">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN DMC1_LIM15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005856">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="Rad51 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0469">Meiosis</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="10">
        <location>
            <begin position="96"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="RecA family profile 2" evidence="11">
        <location>
            <begin position="274"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4366124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="339" mass="36808" checksum="49B3B9E96B6D605A" modified="2023-11-08" version="1">MAPSSVASDDSNNDDGFIVDIDTIQAHGIGAVDIAKLKANGYYTVASVHAATRRNLLKIKGFSEIKVDKVKDALAKCQPSGGGFQTAHELGQLRKRVIRISTGSKGLDAVLGGGFQTMSISEVFGEFRCGKTQLSHTMSVIAQLPKDMGGAEGKVAYIDTEGTFRPERIAQIAERFGVDPETAQDNITYARAVNSEHQMELLNKVAEFFVGNEYRLLIIDSIMALFRVDYTGRGELNERQQKLNQFLSKLTHVAEEFNVAVLMTNQVQSDPGANALFAGADGRKPVGGHILAHASATRILLRKGRGEERVAKIQDSPDMPEKEATYIITNGGINDPEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3J0</accession>
    <name>A0A9W8Y3J0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Multiple RNA-binding domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">MRD1_1</name>
        <name evidence="3" type="ORF">N0V83_008098</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3J0"/>
    <dbReference type="OrthoDB" id="439639at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="249"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="289" mass="32257" checksum="58797838E1C51BC9" modified="2023-11-08" version="1">MESRAGLMCLKITDKELPKSRRQQKAEKAAPKNDEYIPPRKENDLKRKREEAEQDPKLKEFLDVYQAPSKTSIWANGETQGVDVNTSAEETVQAVAVPEPEDESDDEYQVIAKKPKTAPEPTAEAVDQASVPQDNPAMGVEEAAADAGDAMEDVQNEPAGEQGPVSDADWLRSRTNRVLELVEDDEVPPKAMHSDEPPPPQPKVVKHASPEATVDYVESQQQVEPPETQADDAAPSEEEENKIRETGRLYLRNLHFEVTEEELREQFSKHGALEEVSSAFSYLMKLPMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y429</accession>
    <name>A0A9W8Y429_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Dihydroorotate dehydrogenase (fumarate)</fullName>
            <ecNumber evidence="11">1.3.98.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Dihydroorotate oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">URA1</name>
        <name evidence="13" type="ORF">N0V83_006905</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the conversion of dihydroorotate to orotate with fumarate as the electron acceptor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(S)-dihydroorotate + fumarate = orotate + succinate</text>
            <dbReference type="Rhea" id="RHEA:30059"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="EC" id="1.3.98.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Pyrimidine metabolism; UMP biosynthesis via de novo pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.98.1" evidence="11"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y429"/>
    <dbReference type="OrthoDB" id="14784at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990663">
        <property type="term" value="F:dihydroorotate dehydrogenase (fumarate) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04741">
        <property type="entry name" value="DHOD_1A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.26.10">
        <property type="entry name" value="Dihydroorotate Dehydrogenase A, chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050074">
        <property type="entry name" value="DHO_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033886">
        <property type="entry name" value="DHOD_1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023359">
        <property type="entry name" value="Dihydro_DH_chainA_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109:SF1">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (FUMARATE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 11" id="KW-0288">FMN</keyword>
    <keyword evidence="10 11 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 11" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroorotate dehydrogenase catalytic" evidence="12">
        <location>
            <begin position="78"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4367324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="362" mass="38738" checksum="8A224D9B0D5B0E0D" modified="2023-11-08" version="1">MKALTISPPLLNSANPWCTTLEQLRELYNSPHTGAVTTRTSLLDGFPHDPSVHQFAFYNPTTLYPSEPNKDQAGDKETTGSLNTLGYSPIPLKEYLGFVKTISDGLGVIENGKHRKPFIISVTGTVDEVVECYKLISAHQGTVRMVLAMEVNLSCPNIPGKPPPAYDSASLLAYLSALKLEIKRQLEPLEASHPHKGHVHVPIGVKTPPYTYHDQFQSLVDALLSSANAQPTHLPCPISFITATNTLGSSLLLAPQVEGSSGSPHSVFHHTLNSATGTGIGGLAGAPLHPLALGNVYTIKGLLFQHKELEQIQIIGVGGVEDVDGYNRMRAAGAAVVGVGTALGRKGIKVFEEIEEALKKHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y436</accession>
    <name>A0A9W8Y436_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_007670</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y436"/>
    <dbReference type="OrthoDB" id="2874149at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004932">
        <property type="term" value="F:mating-type factor pheromone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001499">
        <property type="entry name" value="GPCR_STE3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28097">
        <property type="entry name" value="PHEROMONE A FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28097:SF1">
        <property type="entry name" value="PHEROMONE A FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02076">
        <property type="entry name" value="STE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="277"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="279"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4367140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="317" mass="35766" checksum="EB3ED3D7C18358D0" modified="2023-11-08" version="1">MQLETNTIVALLIYRLYRYRREFHRLITARNTTKSRFIRLFVICLVIILVYLPYSFWVLGNLSSAIQDPYDWSEVHGPNFNTIMKIPAFGQVSVEKWIQVATGYVIFFIFGTGTDSHNSYKKMLLAIGLGKVFPSLYVMRESGSTTPSTFIHARTWGSQLSTKAKSMFSSKNDTRNDSATNTFSGSTRNNSVVMGSVSRLHNVTTEDTLLSAKHEGAQQVSASKGPSFFKRVFLRRSHRRPVLPLFSRSSITEITDTSSSATRSASPGVQAFVWASEDPVPKENRDQDGVHVLREVHQDRHERDVVEKEGKSGDAWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-06-18" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4H7</accession>
    <name>A0A9W8Y4H7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Actin cytoskeleton and mitosis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">SAC3</name>
        <name evidence="4" type="ORF">N0V83_007548</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="264795at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06503">
        <property type="entry name" value="ATP-synt_Fo_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047092">
        <property type="entry name" value="AFUB_07903/YDR124W-like_hel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045107">
        <property type="entry name" value="SAC3/GANP/THP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005062">
        <property type="entry name" value="SAC3/GANP/THP3_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436">
        <property type="entry name" value="80 KDA MCM3-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436:SF3">
        <property type="entry name" value="GERMINAL-CENTER ASSOCIATED NUCLEAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11001">
        <property type="entry name" value="AFUB_07903_YDR124W_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03399">
        <property type="entry name" value="SAC3_GANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Subtelomeric hrmA-associated cluster protein AFUB-079030/YDR124W-like helical bundle" evidence="3">
        <location>
            <begin position="208"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="SAC3/GANP/THP3 conserved" evidence="2">
        <location>
            <begin position="726"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="375"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1252"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1539"/>
            <end position="1637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1683"/>
            <end position="1742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1764"/>
            <end position="1783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1975"/>
            <end position="2078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1306"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1348"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1586"/>
            <end position="1595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1600"/>
            <end position="1610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1701"/>
            <end position="1738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1765"/>
            <end position="1783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1983"/>
            <end position="1994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="2005"/>
            <end position="2037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="2069"/>
            <end position="2078"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4367018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="2078" mass="232811" checksum="16B853C3AF420355" modified="2023-11-08" version="1">MKTDEFGPDPWIAVNIRDSGRESKAKPASSVTATVKKDQAEDDVALPTPIAATVPLGKIVTADGRELPYYHEIPGFVYQPLPGFEHCFKPRDLSQGTPSTRGAPDSSQTCTRSQESQPAESQQHAAYTERIHQSRQFQGTSARSLYSGSGRSAGSKPQRRRTLKRSRSGVRSIRQYTPTEYSSDEEDDDVDDDRDVKVATEQSFSFYIGDIGELKRFFRRRFDELTTRPLRTIVTAWIKQLEPRRLGGYGKYHKQLPSEQPPGCTPPWWPEDVPYNEPSHLSKGHLQILAVDVMLQHRKIDEVKRKGSWVTKLRQAAQCAIEMTTSELFSSSKRTEFSEKMKERALEFILPSLFDVAQSYEDHLAQYDLYEGTGHTDPGEGRHVTWHAASKPPRSVSQRKRVRRSRVPLSTRVEHHDEESADETEVDDAVSNSFLRRGQVTAGKNTRASEGPKSDDSKSEKTNDAGAPRQQVPVEPTPTATMFPLTQAFPKQEPHPDSSRPTPNTSFNQTMNDLQLNETMSMDMDVKGGDATQYQDPTLFRNMFPFSQPMQYSSSHPGYVFQPQGNASPFSNSTQSSFASGTTSGFASPFPMFHAPYSPQVGNPVFGATISSNNSNGFPYQYEGVFPATPMPHANASFDGLPGGFNVGGQVSISINKTSRNTLASNPTGAQNSTPYPQADYNQRLVHIRKERPAIREQFIRDRLMNPEGQMRLEDSVKIIGICTDMCPEFERVRRIVENDVKPPECTPETEHLTRAHRLPDESRMVKAYARPAAGMDVELVSDIRSPATCLKTVDYLMRRLDGDEFQFLQSWIWDRTRAVRKDLRTQAIEKRPDINILLTCLERTARFHLVSMHQMARSTKEDYSHQQDFEQLNQTLMSLRERYTDNRRANIPSENEAEFEAYRLILAPKFANSNLENDLHFMSNHLRHNSRVKTAIDINQLLKSILDKKSTNFIQCKANWRSLWDLIKSPKVSYLMACAAEVSFNRVRYVVLDSLWRVYRQGNSNKTVVVADWTTDKLKDLLGFDTDKEAVKLCEHFGFVFGRDDTTGQTFLDVSQMGFVQGILGTPSEELSPQSFSARLVESKRHDRAFSAIIQGLSVCEATLKRLIINKRVDTLTPEEEEAMNSLFVPEASSAGANPMGKAPVASALLTGTPLNPFSSPFEPAGNPGVATNTFPSSFQPAGNPGVAKNPFSSPFQPAGSPAVATNPFLKTTSQTQSNHAAAGLTATSIQPGVFDASKNSILFAPSGSGSSIPASNAPTNPFLAKQNTPQTPTPTNPFLMKETAPQPPQSNPFTKFAAPKPEQSVATTTPQPSSAFTFSTQKSNETSALSFTPSGPPPQENAVSQDAERQKTEQKQREAAERQQKEEEARRRAQEAQRARVAQEAEQRRKQAEAEAEQQRQRQMQLDRERQAREEQARRNLEAQQRQAQEEEARRARIRKRESALHSLTEDALLDPEEGLLMQYVEHDIGNMVKEALREEVWRKKRILANKMYDRRQTELKRAALAKIFAHVAKKKKAVQVRERRRRLKAERAQMAIMEEEDKEKQDEVEMSSAPTDLVATKTLSNGDSTFRRPDNQNNAGLTPQRPAAPANGRRARRTEERRKERLSHQGGASEKVPNSNDATRNAAKETATAPVKVTNGNVALAGYSEAHQRAMQRSTAPIDRTETDWFKLRAMGIDPSKHRKRSFDSSSEEEEEAKAEVKRAKLSPSRREPPKAELVKDKLPQIEPPKNDNRELPPATRMTTAEQQLARFRAIKQAFKKSATSPPQLPNGTMSVNGGSSFDGTSSNLIVKARELTIKSPMSEAPLINIQHDFGRSAPFLGLSASTTTRPTSGSSAGATNIDLPAYYHRSSRFVPRHLYGKGSEAVLAYRESYKKSPSNSVGPASTMQSSPVPTQYDYVPQHGYSQEEYTQQQFSERGSNDLEVINVDAEDENAILTDEEGHEEHEEEYEEEHVAYSGNVPSYMRRGMHYPVDQASEMADTEEDEEDVEEGYYKQGHSYAADEEVLEDYDEYSGEDEQDDEDDDMEEDEEEDGGPSQQHFAQPAAHGFARPTMMQPQQNKQPGATEEDAIELSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4I8</accession>
    <name>A0A9W8Y4I8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Translation machinery-associated protein 46</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TMA46</name>
        <name evidence="7" type="ORF">N0V83_006985</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4I8"/>
    <dbReference type="OrthoDB" id="278280at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032378">
        <property type="entry name" value="ZC3H15/TMA46_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681:SF0">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681">
        <property type="entry name" value="ZINC FINGER-CONTAINING PROTEIN P48ZNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16543">
        <property type="entry name" value="DFRP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="170"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="170"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="39"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="273"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="295"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="314"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4367403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="341" mass="38727" checksum="54C4E39F43D557BE" modified="2023-11-08" version="1">MPPKGKGDQPKVKKVAVDKTFGMKNKKGGAAQKQIAQIKATAAAGGSPDEKRKAAEKAQREKEKAAAEQARKETAELFKPVQVQKVPFGTDPKTVLCQFFKKGNCEKGKKCKFSHDLAIERKTEKRSLYTDSRDQEKAEEEEQKKKDNIDDWDEEKLRQVVLSKHGNPKTTTDKVCKYFIQAIEDQKYGWFWVCPNGGDKCFYKHSLPPGFVIKTKEQRAAEKALMANSPLATLTLEDFLESERHKLTGTLTPVTEETFAKWKKDRVDKKQAEEEAKKMREATGRAMFEKGDWQVDSEADSDEEEEEGAWNLESMRRETEAARARKEEERMAAQLGNVTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y598</accession>
    <name>A0A9W8Y598_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin-conjugating enzyme E2 11</fullName>
            <ecNumber evidence="3">2.3.2.23</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">UBC11</name>
        <name evidence="3" type="ORF">N0V83_007974</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y598"/>
    <dbReference type="OrthoDB" id="10253686at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23791">
        <property type="entry name" value="UBCc_UBE2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="15"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="172" mass="18972" checksum="045E005A9A2D37E3" modified="2023-11-08" version="1">MALAANGRANGGNATVAQRLQKELMDIMTKPTPGITAFPDDENMTVWTATIDGPADTPYANLIFKLSMEFAANYPYSPPTVLFKTPIYHPNFDFSGRICLDILKDKWSAIYNITTVLLSLQSLLGEPNKLSAPPMLFSSPLNGQAAELWDKDPAEYKRLVLARHKAPEELDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5Q9</accession>
    <name>A0A9W8Y5Q9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acyl-CoA dehydrogenase/oxidase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007712</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5Q9"/>
    <dbReference type="OrthoDB" id="10254877at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="98" mass="10648" checksum="4C2F73CD094C7789" modified="2023-11-08" version="1">MAPMSTADAELLLAGQIAQLKAHSSIVVQNVVREAVQILGGIGLTRGGRGARVERVWRDVKAIAVPGGSEEIMLDFSIKRAIRVHRVLQNKEKVSHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5V9</accession>
    <name>A0A9W8Y5V9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">26S proteasome complex subunit SEM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">SEM1</name>
        <name evidence="4" type="ORF">N0V83_006891</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the DSS1/SEM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5V9"/>
    <dbReference type="OrthoDB" id="5586203at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13768">
        <property type="entry name" value="DSS1_Sem1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007834">
        <property type="entry name" value="DSS1_SEM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16771">
        <property type="entry name" value="26 PROTEASOME COMPLEX SUBUNIT DSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16771:SF0">
        <property type="entry name" value="26S PROTEASOME COMPLEX SUBUNIT SEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05160">
        <property type="entry name" value="DSS1_SEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01385">
        <property type="entry name" value="DSS1_SEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4367310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="99" mass="11236" checksum="2A51B2C35ED409C1" modified="2023-11-08" version="1">MSGAAQGSSATTKAVVSDTKNGEQPLAQQDKKPAVQLEEDDEFEDFPRRRRLTTELDWTEEETQIPGGNAHLWEESWDDDDTNEDFAVQLREELKKLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5X9</accession>
    <name>A0A9W8Y5X9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005877</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5X9"/>
    <dbReference type="OrthoDB" id="1733656at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040908484" evidence="1">
        <location>
            <begin position="18"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="248" mass="27113" checksum="FD0BD6E4B2B92D3F" modified="2023-11-08" version="1">MRFSSTLVLALPALALAEEQVPLLDKVKGFFNKATAAVSSAIPAAPSAPVDATANKAAAKAAEAIQHPINLENWKEVLTVDPTVSTPTTQEWLIFVTGGNSTCFGLCGNATKAWNTSLPILAAKPNAPKFAYLDCETDNILCNSWSVGAPSLYYFQIPKPLADQSAPVPTVRYRPLNRTSTTTETFKTLIVDNEIEKVEPYEGIFHPFNGQLQQYNLAVPYGYATWAFSKMPSWMPMILISFLSRSFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y649</accession>
    <name>A0A9W8Y649_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ferric-chelate reductase (NADPH)</fullName>
            <ecNumber evidence="3">1.16.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_007291</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 a Fe(II)-siderophore + NADP(+) + H(+) = 2 a Fe(III)-siderophore + NADPH</text>
            <dbReference type="Rhea" id="RHEA:28795"/>
            <dbReference type="Rhea" id="RHEA-COMP:11342"/>
            <dbReference type="Rhea" id="RHEA-COMP:11344"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.9" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y649"/>
    <dbReference type="OrthoDB" id="17725at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF23">
        <property type="entry name" value="FERRIC-CHELATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="10">
        <location>
            <begin position="87"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4366766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="399" mass="44834" checksum="536A59055054C6F6" modified="2023-11-08" version="1">MQSHFSESLLFYWTGIVAITFQAWLTFMSHATIRTLGYEFFKVTHFASVIVFMITFFWHCDYTLTSWHYFVATAAVYVPCFVYPWLRTIFEYRWTQKAHIYVEDNGFTRITIPAKFDWTPGQHCFLRFTSFGILHAVSSHPFTICSSPSGNKNGQSELVFYIRHQGGFTRKLYQHALNHPGVSLSVLVDGPYGGVTPARLESTDHHLLIAGGSGAGWCLPFIEHFIYSAGTSSDEGRTSNPVSLRVILATRDTSSRIWFERTVDQLLTRCSTKNLSSHIQVQVYLTGEAAEKAELPTTTVDPIDALESETTQHKIMHPEIGVATSVPGSEFEGRPQLSLMVREEVARAAEARGSLGVYVCGPVTMQNDVRNAVAEENLNIIRSARSGGVYLHSEYFSWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6F5</accession>
    <name>A0A9W8Y6F5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1275 domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007714</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6F5"/>
    <dbReference type="OrthoDB" id="5288586at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010699">
        <property type="entry name" value="DUF1275"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37488:SF7">
        <property type="entry name" value="DUF1275 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37488">
        <property type="entry name" value="DUF1275 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06912">
        <property type="entry name" value="DUF1275"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="28401" checksum="04CD5E223D743562" modified="2023-11-08" version="1">MQIHQSRLSGWRKPIDKQYADIPLCLCSFVSGLCDSASFNASSVFVSMQTGNTLFLAIGAARLPPNVPYMWLRAITSIGCFMLGCFCFSKLRHVEPLRKGTLAFNFFTQGVFIFVAAALAQSGVVSAMADLHVAERLAGELHFDFNLLIPIALLAFQFGGQIVTSRMLGYNEVPTNVLTSLYCDLFSDPNLISPWSSNPKRNRRVFSAVLFFLGGLSGAWLAKSDAGMGTALWIGGALKLVIALAWLVWKEKDVAFEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6M5</accession>
    <name>A0A9W8Y6M5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="10">2.7.8.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PGS1</name>
        <name evidence="13" type="ORF">N0V83_007566</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Functions in the biosynthesis of the anionic phospholipids phosphatidylglycerol and cardiolipin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>a CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate = a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12593"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60110"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the CDP-alcohol phosphatidyltransferase class-II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.5" evidence="10"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6M5"/>
    <dbReference type="OrthoDB" id="10250191at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09135">
        <property type="entry name" value="PLDc_PGS1_euk_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09137">
        <property type="entry name" value="PLDc_PGS1_euk_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016270">
        <property type="entry name" value="PGS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586:SF1">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--SERINE O-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000850">
        <property type="entry name" value="Phospholipase_D_PSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="12">
        <location>
            <begin position="136"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="380"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4367036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="478" mass="53303" checksum="365A4F8A5D356E51" modified="2023-11-08" version="1">MLATITNDLDKIAPSFEIQPEQIQIIQTPAEFYQTLKDKISKAQRRIYLSTLYIGKTEHEFISTIRAALLANPNLRVSFLTDALRGTRETPDPSSASLLTPLITEFGPERVEVRMYHTPNLTGLRKKYIPKRINEGWGLQHMKLYGIDDEIIMSGANLSSDYFTNRQDRYHLFRSKDLTDYFHTIHSGVASLSFDFQPSDAPGSGGYSMTWPSSNPAPSPLEAPNDFRAAASKHLSHLLTPSNSPAKLQAVASSSPTTTVYPILSLVPLLPTSTELPALTTILTSLSRAPLHPSTWTFTAGYFNMMPSFRRLLLSTRPSSGTVLTAHPHANGFYGSKGVSGMLPDAYTHLAKMFLRKVRHEGLDGAVELREWKRGWLGRRAGGRTSTAPPREDGNEDPSLTVVGSSNYTKRAYELDLEANVIIATTDPGLRRRLGEEVRWLGEYAEKVDEREFEKPERKVKLSVRLSMWIVRVLGGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6N9</accession>
    <name>A0A9W8Y6N9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_006270</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6N9"/>
    <dbReference type="OrthoDB" id="5302359at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000017">
        <property type="entry name" value="2-dehydropantoate 2-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000424">
        <property type="entry name" value="Meiotically up-regulated gene 72 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051402">
        <property type="entry name" value="KPR-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708">
        <property type="entry name" value="PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708:SF25">
        <property type="entry name" value="PROTEIN PAM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketopantoate reductase N-terminal" evidence="2">
        <location>
            <begin position="10"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="3">
        <location>
            <begin position="200"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="494"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="636"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="405"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="526"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="678"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="778"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4369185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="815" mass="87552" checksum="2871CECDF9067EBF" modified="2023-11-08" version="1">MAPAVPRLRILSVGGNAVSAFLSWRLQATNACDVTLVWKSGYDSVAQYGISFKSELYGNERFKPYAVVRTPEDAANSSKQPFDYVLLCVKALPDVYDIANIIESVVSPQHTCILMNTTHSLGVESYLEQRFPTNVVLSLVSGAEISQLGASEFEHKGATDIWVGPANKNPAIPVQIQSDMAEALAMTLSSGQVDCKVSPNIRQQQYERMIGPIAFHPASIIFETPNHSELMEKVGVRALITGVLDELLALAKAQDCHFPADFRATTLQQMTRPQETNSTMWMDFEAKRPIEIENYLASPLKLAQENGVSVPRIETLYATLHHLNTVNRTRPVAPPTPSPQNGMQPLPPRLSSAPLPRGPQPQAGPGPVMNGNGPMKGGPRPGSRAPSINGAPPMMRRGPPPGPMNGPMNGPMNGPMNGYPPRMNGAPNGQRRPSLEDNTLEEFSHLMHYDNIVEDGAPNGGPYESAGNSSTNLSIRERELMLRQRELQLREQELNMRRGPGGPGGPGGPGGPGRGRPMPPPSMAGFDDDDDDGDDYFDPMGGRNPGSMIDPDNFDMMSVTSRRNRKMPSASQMRKNPESFAPGPNGRSRNPFARPGMNKNRSSTQMMNSIPGLHTNIMNDPLLGYSSNRYGDVDRGAMGAQSRTNSLTASRLDELQMGGNYGAYPPNPRRTSQSPGNPLSPGPRPNGRPSPPNGYAPNGMPPNGMPPNGMPPNGMPPNGMPPNGMPPNGMPPNGRPSPPGMRQPVPRHPPGMGNAVAPQQVEQQAGVSNLYPPKSRPQVRSLTGSASGSSGHIDSENSAHSSQSSLGPRPPLGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6V5</accession>
    <name>A0A9W8Y6V5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Succinate dehydrogenase assembly factor 4, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005909</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6V5"/>
    <dbReference type="OrthoDB" id="201362at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="22996" checksum="88AA6F7BFEE711A2" modified="2023-11-08" version="1">MNLSPQLFTISTFRHFNLYNLPIHSNFQPVIYTMATTQRTLVHLPRQLSRRTFTTTRTRLSQSPFGSGPAPPRLPKGEQELFEKLQRQSTGAFSTPRTSDITTPSPPSTPNIRVQINQSPDSTPSHSSQQQQQQLPQSGRMQINQSPDSSGSDSDAVQEIRFEGGKEIREGEDLHPNMRRGAPPEFEGDVNPKTGRWVGRRMSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6Y4</accession>
    <name>A0A9W8Y6Y4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_007716</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6Y4"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17502">
        <property type="entry name" value="MFS_Azr1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000012">
        <property type="entry name" value="MFS toxin efflux pump (AflT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF177">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4367186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="399" mass="42489" checksum="2CF92F66751CC5E5" modified="2023-11-08" version="1">MATIIGTAIPKITDEFHGLSDVSWYAAAYFMTFGASQTSAGKIYKYFNLKWSFLVSMFIFEIGSLICGVAPNSKTLVVGRAIAGLGAAGLSVGGTSIVSFTVAPVKRPMMMGVIGMTYCLAAVLGPLLGGAFTDRASWRWCFYINLPIGGVAAVAVFFFFHLPAAAAPPNIPWHKKLLHIDPVGVVLVLGSITCFILALQYGGITHPWGSSVVIGLLVGFALLAIALVGWENWLGEYAMMLPRLYRQRSLYTTAPFQFLFMGSYIVILYYLPIYFQSILSASPIRSGVNNLPLVLAAAVFAILGGAVVMRTGRAQQVMAVGSMLSTVGLGLIYTFDIGTPTAKWVGYQFFVGAAMAFGIMHGLSVAQASVGPEDLSAVTANLLCTSLSVPLSAYFKVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y715</accession>
    <name>A0A9W8Y715_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PHO85 cyclin-5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">PCL5</name>
        <name evidence="2" type="ORF">N0V83_006292</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y715"/>
    <dbReference type="OrthoDB" id="286814at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF36">
        <property type="entry name" value="PHO85 CYCLIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="459"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="314"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="520" mass="57184" checksum="994C45DC956C28DB" modified="2023-11-08" version="1">MVEVIWQLSEPKSRCETASGRGVLPLRTFIQETLRRSRTSYSTLQVALYYLILIKAHLPKHDFTMSQPEDASLRALQCGRRMFLAALILASKYLQDRNYSARAWSKISGLKVCEINTNEMAFLEAVNWKLHIVDPVWEKWQEVVLRHTPTNPPSSPGAPILNTWKDVIPILTPELDIIELTPKTPVTPIRPQLRPQLSASPYRYPSSDGYGSQESTPTPSRYQVPRFLEPKPDLLPPTPSLARLGPLPTPSLTPQSIASSTPAASALAFGSRRPSMCSAMQQAQCSSLARTCVDQFNPSIRNGLEAYHFPTRRPSLAPSNSSLSSSPESMVSDNSRSSRASSISSVSSAGWAPNQSQAKLARLATCRNARLPYPALSKCKEQYEYAPSEPMSSPDLESFHIDDSDSASVSVESPVAIATASRKRGRSSVDLQQNVRHLLHSSRSAFSLRGSQTVLPDRSVAQPSLVSRSEPKGLQSPKDTAPDCCRFPIQKDSGKKRTCCATEAAQAFRPQGPGMWKGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7H7</accession>
    <name>A0A9W8Y7H7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome c oxidase polypeptide V</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">cox5</name>
        <name evidence="14" type="ORF">N0V83_005548</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome c oxidase IV family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7H7"/>
    <dbReference type="OrthoDB" id="186013at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00922">
        <property type="entry name" value="Cyt_c_Oxidase_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.442.10:FF:000002">
        <property type="entry name" value="Cytochrome c oxidase subunit V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.442.10">
        <property type="entry name" value="Cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004203">
        <property type="entry name" value="Cyt_c_oxidase_su4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036639">
        <property type="entry name" value="Cyt_c_oxidase_su4_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10707:SF10">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10707">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02936">
        <property type="entry name" value="COX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81406">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00081365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4369784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="188" mass="21058" checksum="7F59B363E84D838C" modified="2023-11-08" version="1">MLRSSLARASRSSAAVIPSTATRTAIPRAAPVACQVQQTRQAHAISNPTLANIEKRWEEMPPQEQADLWMSLRDRMKTDWKELTMQEKKAAYYIAFGPHGPRRPAPPDEGRNVFFLTSAVIAGAFALFAFTRMFASPIRPRTMTKEWQEASEEYLKNQGSEPITGFKGMLVQSKSEKQLGSGEKLNDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7R6</accession>
    <name>A0A9W8Y7R6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ADE17</name>
        <name evidence="14" type="ORF">N0V83_005683</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Bifunctional enzyme that catalyzes the last two steps of purine biosynthesis. Acts as a transformylase that incorporates a formyl group to the AMP analog AICAR (5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) to produce the intermediate formyl-AICAR (FAICAR). Also catalyzes the cyclization of FAICAR to IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(6R)-10-formyltetrahydrofolate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + (6S)-5,6,7,8-tetrahydrofolate</text>
            <dbReference type="Rhea" id="RHEA:22192"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="2.1.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:18445"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="EC" id="3.5.4.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PurH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7R6"/>
    <dbReference type="OrthoDB" id="6017153at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004643">
        <property type="term" value="F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01421">
        <property type="entry name" value="IMPCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.20:FF:000003">
        <property type="entry name" value="Bifunctional purine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1380:FF:000003">
        <property type="entry name" value="Bifunctional purine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.440:FF:000001">
        <property type="entry name" value="Bifunctional purine biosynthesis protein PURH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00139">
        <property type="entry name" value="PurH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024051">
        <property type="entry name" value="AICAR_Tfase_dup_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024050">
        <property type="entry name" value="AICAR_Tfase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002695">
        <property type="entry name" value="PurH-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005492">
        <property type="entry name" value="PRK07106.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00355">
        <property type="entry name" value="purH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692:SF0">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN ATIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN PURH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01808">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000414">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00798">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MGS-like" evidence="13">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4369919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="597" mass="64964" checksum="A050DB1AC901ABAA" modified="2023-11-08" version="1">MLTATAILSVYDKTGLLDLAKGLHECGVRLLASGGTAKMIRQANFPVEDVSAITKAPEMLGGRVKTLHPAVHGGILARNLDSDDSDLSANSIEKIDFVVCNLYPFSETVARINVTIPEAVEEIDIGGVTLLRAAAKNHARVTIISDPSDYHDLLTELKKDGEVSEKSRQRYALKAFEQTASYDQAISDFFCKKYAGDGDQFLPLRYGANPHQKPATVSMADKPLPFKVLAGAPGYINLLDALNAWPLVQELSKALNYPAAASFKHVSPAGAAIGVPLSEGERKVYMVDDIEGIENSGLAQAYARARGADRMSSFGDMIALSQEVDVPTAKIISREVSDGVIAPGYTAEALEILKKKKAGKYLVLQIDPEYVPSVEEARTVFGITLKQHRNDAKIVPADTFNTVIVPKNSGSLPESAQRDLTVATIALKYTQSNSVCYALNGQIIGLGAGQQSRIHCTRLAGDKADNWWMRFHSRTLGLKFKKGTKRPSKSNAIDLLCSGLVPESGIEREDFEAHFEEGQVPQPFTAEERKEWLSKMEGVAVSSDAFFPFIDNVFRAHRSGAKYIAAPTGSQNDGAVMETSEKLGITFVEQHIRLFHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y874</accession>
    <name>A0A9W8Y874_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="9">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">GAS4</name>
        <name evidence="11" type="ORF">N0V83_006527</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Lipid-anchor</topology>
            <topology evidence="1 9">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y874"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000032">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF4">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 9" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041012444" description="1,3-beta-glucanosyltransferase" evidence="9">
        <location>
            <begin position="22"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="422"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="429"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4368171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="477" mass="51616" checksum="5E7B9614C356DD14" modified="2023-11-08" version="1">MPSLVRALALLPALFSTIANAVHPVEVQGQDFVDTVTNKRLMIIGVDYQPGGQAGYKPQEGQDALTNADVCLRDAVVMQKLGVNTIRVYNVDPTLDHSSCASIFNAAGIYLILDVNSPLSGESINRAEPWTSYNSEYLNRIFGIVENFKNFPNTLGFFAANEVMNDLTTAEFNPQYIRAVQRDLKNYIKKHASRTIPVGYSAADVREILEDTWAYMQCNHSNDASSSDFFGLNSYSWCGGDATFQSSGYSDLVTMFNQSAIPVFYSEYGCNKVQPRVFDEVAALYGNQMTSLSGGLVYEYSQEEADYGLVTLNSNGSVSLRTDYDNLQGQFNKLDISLIQSTNAQSTQIKAPACNGDLISADNFSKNFTIPDVCPGCQDLIDNGIEDPKNGKLVDVTETKVKQEVYGSTGVQIQNLELTIVANDGSNTPGGDTTSPSASTTSTAPSPSASKKGAAGRLSTTGAWVWACLLVVGVMLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8B5</accession>
    <name>A0A9W8Y8B5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005618</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8B5"/>
    <dbReference type="OrthoDB" id="3720847at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="515" mass="59212" checksum="BEECF876AA7EFDFE" modified="2023-11-08" version="1">MLRLTDLPSELLSSIFGHLSGKEQHALTTTCHVFHTLATSHLYRNIKFTATKSRSCARKLALLLRTLLERPRLSPKVTAFALSGPQQCWEKYDPWPENNERKTIVKLWGFDKTTQTQSLLTPAMFHHFMDDDMQINQAELRGRSKDALATLIMTRLMNLHTLELGDGFLRHSIFLPQILKRTDYLFPDLERVVLGDRRRDRDSTVSYTDLSLIRPVFYSSTIKSFECTMTQPWKFCWTETTAPWNTNLTTLRLFRTNIDRSTLRQLLSAVPYLKSFHHEQEIAFNESTPTAPSLAPYLELDGLNSALACVRHSLEECQLVLRLAPGSIPASQVVSSGIRFPAIEGTLSILKDMPRLKKVEVPMIMLFGWSPRPSVKLEQLLSPHITELTLRDDLVPYCYWSRPSSRERRIARIGRYIADRATHAPKLRMLKVRLTGGKECLRDAVQELSMRTDDKDVQSMIVRGTKSETYCWRFEKAKAVASRPELGERVDSVLREDDCVRPEREFFTKLSRLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8K6</accession>
    <name>A0A9W8Y8K6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004892</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8K6"/>
    <dbReference type="OrthoDB" id="5429770at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053175">
        <property type="entry name" value="DHMBA_Reg_Transcription_Factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38791:SF5">
        <property type="entry name" value="TRANSCRIPTION FACTOR DBAG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38791">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="541" mass="61004" checksum="0C225223EC0CDB81" modified="2023-11-08" version="1">MPNCDQKRPSCSQCLRVGKECHGYRDMLSMMFKNESDVVAKKAEKRYEALAKQEVPTTSKRKAQQTSDYDLPSRSSTHMVHYMRYPTPESLTWEIIPSIEEQAQGFFVSNYVAQPAIVPRGQFEWVTELLARPDTEEILRSSVNAASLAGLATSTKSPMIMQKAQAAYVKALRITNSALRVKATAVKDSTLISVIMLGMYENMVSQNKQSIQAWAKHVAGACALLSLRGKEQFKSDISRRIFHQFYGVVLLVALETGTAVHEGMHELYETLHPTSDYDVHGRQWTTKVVNVIHDAINLSQDKRSNPVTMVNTALAIDNELDEIKSLMPSIWHYETIYLEQPSEYQFGDCYHVYVDPWIAQMWNNLRSCRMYLYKIMRENVTKGLELYDPPLFSVQEIAPRKEAAEHVMRTTASRVVASVPQITGMIPFPTVTSLLSKPPAASGQQRHPSKYTIHAPGTFLDPAKSTGMVHLIWPVYAVGQSDLSSPKLRQWCIETLHTIALQIGTRQAIVLADELKEIQTSGMRSEVAKSSDCFFNRVTMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y937</accession>
    <name>A0A9W8Y937_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF3669 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004415</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y937"/>
    <dbReference type="OrthoDB" id="2993351at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022137">
        <property type="entry name" value="Znf_prot_DUF3669"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40780">
        <property type="entry name" value="DUF3669 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40780:SF3">
        <property type="entry name" value="DUF3669 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12417">
        <property type="entry name" value="DUF3669"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3669" evidence="2">
        <location>
            <begin position="405"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="494" mass="56224" checksum="FF4DE2212D363F39" modified="2023-11-08" version="1">MDASTRLYQRLGQIPEDPNDPESLDELLVCAFGAFARYYVLWKTRGGTFRQDSYDLPIALRDWLYSVDGTGRDFATLQVVFGRGEEYFASDQYSKLEYKEPEAKRPDEDEEKLDKQTLRRSRTVRPSQSYVTSQSTRVGSSAEDSQSPILSPAKKADGLPGSISGDEYEPKPAAQDYRCIGKGFCGSVWALEGNPGHACTAIKREDGGPGRSITNDYINHLRVLQAALQHPPATPLSVPQCHELIQPTDEAWWLQRLHQFPPGYSACRALLSERIPRVPRIISNKIVDLFCDGNAPLSDFVKSNLDDDACLIRPYLGRRRRHRQETSTSRFQRFSLRNVPLHIDQMEALGLDIRAYVETMADALALMHWGAQIDANDVEFVLAPPRGEPLSSSVFNCEYLGIHCMWILDFDCCRPLSMDDAGIEKACQAFFKNDPFYPRPESEEAADKELWKVFKQRFLVTSRRILEDVGQDGRYLVEKLVDRIEEEGRLRRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9B1</accession>
    <name>A0A9W8Y9B1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 18">Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial</fullName>
            <ecNumber evidence="5 18">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">SDH2</name>
        <name evidence="22" type="ORF">N0V83_005203</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="18">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="18">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="18">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 18">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Mitochondrion inner membrane</location>
            <topology evidence="1 18">Peripheral membrane protein</topology>
            <orientation evidence="1 18">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="5 18"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9B1"/>
    <dbReference type="OrthoDB" id="1696654at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000007">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1060.10:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase iron-sulfur subunit SdhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050573">
        <property type="entry name" value="SDH/FRD_Iron-Sulfur"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004616">
        <property type="entry name" value="PRK05950.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921:SF29">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] IRON-SULFUR SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE IRON-SULFUR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13534">
        <property type="entry name" value="Fer4_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="16 18" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="8 18" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="14 18" id="KW-0408">Iron</keyword>
    <keyword evidence="15 18" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13 22" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="20">
        <location>
            <begin position="88"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="21">
        <location>
            <begin position="209"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ4370682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="306" mass="34797" checksum="87B2FA3ECADD87C5" modified="2023-11-08" version="1">MACTRAFSRLATTRTAVRPAIFSRGFASVNDVHAREPISKVADKVVPDPARTPVPESKTSTIKEPEPSQDAKTKTFHIYRWNPDEPSAKPRMQSYTLDLNKTGPMMLDALIRIKNEVDPTLTFRRSCREGICGSCAMNIDGVNTLACLCRIPTDTTKESRIYPLPHMYIVKDLVPDMTLFYKQYRSVKPYLQRTTPAPDGREYRQTKEERKKLDGLYECILCACCSTSCPSYWWNQEEYLGPAVLLQSYRWIADSRDEKKAERQDALNNSMSLYRCHTILNCSRTCPKGLNPALAIAEIKKSMAFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9J8</accession>
    <name>A0A9W8Y9J8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Glutamate decarboxylase</fullName>
            <ecNumber evidence="3 9">4.1.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">GAD1</name>
        <name evidence="11" type="ORF">N0V83_006199</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 9">
            <text>L-glutamate + H(+) = 4-aminobutanoate + CO2</text>
            <dbReference type="Rhea" id="RHEA:17785"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="4.1.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the group II decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.15" evidence="3 9"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9J8"/>
    <dbReference type="OrthoDB" id="5152799at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004351">
        <property type="term" value="F:glutamate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000017">
        <property type="entry name" value="Glutamate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.50:FF:000001">
        <property type="entry name" value="Glutamate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010107">
        <property type="entry name" value="Glutamate_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01788">
        <property type="entry name" value="Glu-decarb-GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321:SF3">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5 8 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="514"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="534"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4369115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="546" mass="61082" checksum="2424267CE9FAACD7" modified="2023-11-08" version="1">MSLARHVDPDELIQTLHDHPTHKAGRHGSNRAVSHTTPYSSRYAASTELSKFRIPQDGAPADAVHQLLKDELDLDGRPSLNLASFVGTYMEKEAEQLMIENLSKNMSDADEYPAMMDMHARCVSIIANLWGAQKGEKAIGSATTGSSEAIHLGGLAMKRRWQEKRMAAGKDTSKPNIIMGANAQVALEKFARYFEVEARILPVSEGSSYRLDPELVKQNIDENTIGVFVILGSTYTGHYEPVEEISDILDDFEKKTGNDIPIHVDAASGGFIAPFTHAKAGKKWHFELPRVKSINTSGHKFGLVYAGVGWIIWRDESYLPKHLIFELHYLGGTEESYTLNFSRPGAQIIAQYYNLIHLGFSGYRNIMENTLANARLLSRALEYTGWYRCVSDIHRKKGDFKFEEGKKQYDEGDTSADYNAGLPVVAFALTDSFKRDYPHVKQEAVSNLLRAKQYIIPNYPLPPSEDKTEILRVVVRESLSLDMIDRLVTDICSVTEMLMKTDAVDLAAFQPGTPSVEKQHANKGLKAEHRHKAERPMSDGVHRTVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9R4</accession>
    <name>A0A9W8Y9R4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004594</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9R4"/>
    <dbReference type="OrthoDB" id="2862635at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070007">
        <property type="term" value="F:glutamic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13426">
        <property type="entry name" value="Peptidase_G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.700">
        <property type="entry name" value="Peptidase G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000250">
        <property type="entry name" value="Peptidase_G1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038656">
        <property type="entry name" value="Peptidase_G1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37536:SF1">
        <property type="entry name" value="ASPERGILLOPEPSIN, PUTAITVE (AFU_ORTHOLOGUE AFUA_7G01200)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37536">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_3G02970)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01828">
        <property type="entry name" value="Peptidase_A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00977">
        <property type="entry name" value="SCYTLDPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040836296" evidence="2">
        <location>
            <begin position="17"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR600250-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="262" mass="26964" checksum="28BDB07131EAC396" modified="2023-11-08" version="1">MKFTLILAAAASIVAAAPGAELVQRSTTDQLVARAAGRNKFTKPNVRIPKASNANNTEVEYSGNWAGAVSIGSGYNKVVGTITVPKVSGSRGAAASAWVGIDGDTCQTAILQTGISFYADGTFDAWYEWIPDYSYSFSGFSVKVGDKITMEVDASSKKAGVAKLTNASTGKSVSHTFSSSSTPSSLCETNAEWIVEAFQENGSQVDLADFGTVTFTGASATGSSGTITPANAEILDISPDGGETVYTSCSASGSTVKCTYTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9Z8</accession>
    <name>A0A9W8Y9Z8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_003730</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4371957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9Z8"/>
    <dbReference type="OrthoDB" id="2102561at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000140">
        <property type="term" value="F:acylglycerone-phosphate reductase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05374">
        <property type="entry name" value="17beta-HSD-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169:SF6">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4371957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="30412" checksum="17842F041346F270" modified="2023-11-08" version="1">MAMIQKKTALITGCSTGSIGWAMTKVFHEQGFHVFATARDPSKAADLSSLSDMEVLELDVTDPQTISRCKDIVTRGTGGKLDVLVNMAGVEFQSPLLDVDIAAAKELYDVNVWGPLAVTQAFAPLLIEAKGVIVNQSSIDAVLSMAWAGIFASSKAAEARLSETLRLEMEPLGVRVVTIMCGSANTPMFSKPSGRMKLSETSHYYNVQDTAYEERMEHQGKAMKVEVLADKLVKDILGGAKGAIWHGALASLTRFMTWACPTWLVDKMVNGERGLDLVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAW2</accession>
    <name>A0A9W8YAW2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Manganese/iron superoxide dismutase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004921</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAW2"/>
    <dbReference type="OrthoDB" id="275227at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S26, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="2">
        <location>
            <begin position="130"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="2">
        <location>
            <begin position="232"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="295" mass="33034" checksum="7EFF7429279042E5" modified="2023-11-08" version="1">MAIIRCLARRPSALQWAARASSRIPSFARTLYAVPTLGNHVELQKNGVPGLLTAKGFQIAYTDYQQHMIDELNESTAGTTAENQDTKSLVIEFARDPTKAYGFNVASMAFNNHFFFRGINTNLNVQSRPSSDLEVQIKKDFSSRDTLRETFLATADAMFGPGFVWLVQTNDTQFGNLRILPTYLAGSPLSGAHYRQQPHDLNTHNADSYQALNKVGAFGAAAKQNPGPKKPLGGVDVVPLLCVNTWEHVWLHDYGVRGKRRFLEAWWDRIDWDLVGQHATLSPRQGQASRQFHFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBD5</accession>
    <name>A0A9W8YBD5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_005164</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBD5"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11476">
        <property type="entry name" value="SLC5sbd_DUR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1730.10:FF:000006">
        <property type="entry name" value="Urea active transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031155">
        <property type="entry name" value="DUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154:SF2">
        <property type="entry name" value="SOLUTE SYMPORTER FAMILY TRANSPORTER (AFU_ORTHOLOGUE AFUA_6G03200)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="61"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="293"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="355"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="399"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="459"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="498"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="604"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="10">
        <location>
            <begin position="648"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="544"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="544"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="567"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4370643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="712" mass="75890" checksum="88132141897FA4C0" modified="2023-11-08" version="1">MADTTKTPLPVGAGYGVVVGIGFFFAGLMMFISHLQNRYTQYSTHQSEEFNTASRSVKPGLIASGIVSAWTWAATLLQSSTVAYTYGVAGPFWYAAGATVQILMFSILACKVKQNAPRCHTYLEIVKARYGTVAHLVFMVFAFVTNILVGSQLLLGGSAVVTSLTGMPVYAAVFLIPVGVCAYVILGGLRATFLCDYSHTLILMIIILYFMFNAYAVSPLIGSPGEMYDLLKKAGSQRPIEGNKDGSYLTLKSNYGLVFGVIQLCSGSGTVFLDQAYWQRAIASKPSTAVRAYLLGGLAWFAIPFGFSTTLGLAAAALTDNPRFPTYPNVPTSGQISSGLAAAFAAETLMGQGGAVALLIVLFMAVTSCASAELIAVSSLLTFDVYKAYIQPKATPKQLIFVSHVMICVFGLTMSVFACIWNAASIDLGWLFLVMGLLIGGAVFPTAFAITWRKQSKAGAISGCLAGLAAGIIAWLTTAKHYYGAINITTTGLEYPTLAGNLAAIMTGLIVSVTVSLIKPANFDWEVTRSINAAVTDGMATTQDGVVTPRNASTPDLSDGGEEKGAAKSSAAPPATTPAELPAHLAPTAPEEDDPRTLRRAFKLACISAFVLTFIMDFLFPMPIFFSEYIFSKGFFTGWVVVYDEQVVIVCDDKKVSISKNLFDALSLLALVKPDGFIWADGICINQSDDREKSSQIQMMGQIYASAVGICI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBR2</accession>
    <name>A0A9W8YBR2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBR2"/>
    <dbReference type="OrthoDB" id="3903581at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="660"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="949"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="456"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="701"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="956"/>
            <end position="969"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1005" mass="111439" checksum="C21A16037367A2CC" modified="2023-11-08" version="1">MFPCRLVHQDCLVGPEFAERLNLALQRSGSMSTQQQSQTDLFWDSEALESTRSVSTTAASRAASDAGKQHATLALFETVITSYPPVLESLLSQLSTVAVLQLYHTSPHLRDFLRHYPLAWKTLSFRLPQPAVTNVGSPGNETPDSRERQSKAYSFDALLKQIIAPNGTRLTSLDLCNTAVTGIALVGSVLAPRIDILQHLSVRGCKNVSIKYHIVPFLEPYTLKDAPWMQKDLALKSLYTYRCRHHRRRPYLPSSLIRRDSDSDPTHQLIEICHQLGIWTDTAWCPSPGGRCYRRKDYHAGRAGPQNMEVWVPFDRLWRSSNRIGPAEGSRKLGSHDGRLWEIAETGEDGEPLGPENGEYAGEGKHVPAHLRKSHTTFIEEVKCAQCDDVILERCESCSVRMHCMGCRKTLCASCAFNRPIPRKRAKTRHFANLAFGNSNTLGSTLGQAAGSSSSLEDRQREDKVDGNRFWWAPGATRSPNLMNESAHDSDDSDSEDGGNLNNGMPVPPANREPPKLNMHWCCLEPIFSGGGGIAVLGTGLGGRGADKIRAAPLPRTKEFEDPDFSNQLRPVDYIRELKNNGLYEYVLGEDVDILAYLKQDTLDLQQQTCPRGLCHDCYRSFRWKVSCRACKNPICKEHDFRALKVRKCGYRDMHTEREHVRAHNEPLQRLVIPDFNPNRASTPDPERTPTASSRNDHYSTADNQSESSETPMSQSQILVAPAAPASAEMSATTSFNSVSTSDPFPLAASLSFVPQCAALNPACTCTFCTVNYHCPTCARKPNVRAKCRLEAEVKAKQEAELRAMAKIEKDREERGQADELAGAMHEFFAAVYQDDMAPEDVLPCDTRAPSSSDPPIPSPDSITVTTEFGVVEEPANITTVSVAGPSPYHHAYDSHLDEENNVEMTEDLHEDLQTLQQNLEHMQLLPAHMTMTPLLQPTTVLHPTMEVQAGHNPDEDQDDVNDTGEEADIDTDDVNADAESAFIEEFIGEAQADGLLTPTMTPIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBX8</accession>
    <name>A0A9W8YBX8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_002501</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBX8"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000134">
        <property type="entry name" value="MFS sugar transporter protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF31">
        <property type="entry name" value="HEXOSE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="178"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="338"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="23"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4373762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="518" mass="56124" checksum="07825DF68A468B08" modified="2023-11-08" version="1">MADTLGRDMRFVPRSSNVISVLLVAASCVTATTLGYDGSMMNALNILPSYTEYFSLNTTTLALNTSALWIGGAVAGIVYGPITDILGRKKALFWAAVLTIISAIIQAAAQNIAMFVVSRILIGFGTGASAVAGPTYLAETMQLKHRAWGLGVFYDCWFVGGLISAGVTYGTAKMDTTWAWRLPSALQGLFSIICIVILPFMPESPRWLVHKGYNDEALDVIALTYANGDRANPAVLVQYQEIVDTLNYEKNTGETLSVKQMFKTPSARKRTILSLSVAVITMLSGNNIISFYLGTMLENAGITDSHTQLEINIILNAWSLCIALFGTVFANRLGRKKLAAISTAALTIFLFMFGGLTAKYGTSANTAGIYGTVATLFLFQGSYSFGLSPITVMYPPEVLNYPIRSNGMGLYTFLVNGVGLLVTFAFPYALDAIGWKTYMINGAWNVLEVLFILFYWVETKDKTLEEIDALFEGEKHSDVVDLEIVLNGKLDASNVLQGIDVPINSNEVPVTSKAVKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC39</accession>
    <name>A0A9W8YC39_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tyrosyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MSY1</name>
        <name evidence="10" type="ORF">N0V83_002739</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC39"/>
    <dbReference type="OrthoDB" id="337870at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000227">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024088">
        <property type="entry name" value="Tyr-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032005">
        <property type="entry name" value="TyrRSs_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766:SF0">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766">
        <property type="entry name" value="TYROSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16714">
        <property type="entry name" value="TyrRSs_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosyl-tRNA synthetase C-terminal" evidence="9">
        <location>
            <begin position="428"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4374000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="555" mass="61950" checksum="8C08A9F61C369FED" modified="2023-11-08" version="1">MLKLLEERGFVKDVAGGRQTLDWLLTEKRIGVYVGVDPTAPSLHVGHLLPLMALYWMYLHGYYTVSLLGGGTVQIGDPSGRTTARSRQGADVQSMNVESIQSQLEKLWTNVKCLGVRHQYSQSTSRRRDILNNRKWLEKLSAVELMRDLGSGMRLGPMLSRDSVKLRMESGEGMAISEFSYPLFQAYDWWSMYKNQGVQLQIGGSDQYGNIIAGMDAVSHMRKIYRMSNGTEREDDPRVATYGLTTPLLTTASGEKFGKSAGNAVWLDRQMLNSFDLYQVRLVRFPSGALLMRLPQYFLRTADDDVERYLKLFTFLPLSDIELLMAHQRQDESQRKAQHILAREIVELAHGAAEAKKAEAAHKEAFSHGTNTFPLGVLRNTLAGARSTDTPAIEEMSKRERDLYTYKQAFAASSTVQANSGTAHEQPKNENNHIVTLPVTLLRPGSFPQVLHAAGLAGSKSEAHRLIAKKGAYVVVPNSGSSENPTALRWATIEAGALADPNHFLVDWEALVLRSGKSRIQMCRIVTEEQFDAEGLTYPGWEELKAQRINATKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC42</accession>
    <name>A0A9W8YC42_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC42"/>
    <dbReference type="OrthoDB" id="4716584at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="226"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="661" mass="73970" checksum="1876453CC0CDE08E" modified="2023-11-08" version="1">MASEAPPRRFKVEPIETTSKSSKKPSQEDQVERPKLVRKFAPEPIETSKKTHRKFVPEPIETTKKVQRRFAPEPIDTSSRNSRQKFAEEWQEQTSPSPRRFKPEPVETTTSSNRKPILKRPAKKFAPQLIETAQRSRKAGDYAPALLPSDKTEATPDEVISARKARILGIPPTPPTNTPTLDLHQNPLFLEIQRNASPLARRRSYFSSSQHSFRIPDLDPIESSESEASSPPSPTTSPSITSDHSFMYKEATRRRESVDDRSSGYLLELAAKAAEKQLREQAMAAFPNDDYHEPVDHFVDRDVEESEPSTAGRKSGRGSSYTEVNWELAAMRNYQEKQEAEKEKQKQDASPSKPKEQIKPAYNPFGNPAGFLDTATARKLAEASRDPELERMRKGARPPMLGKEIKFPRCASPEPSRFDPTQGCDAVRTAMCYLSEQSKAASEKGESLWCGKGNGRQTSQTPSLWSNANSRASSTHGLWGGFCVNGGDKSPRGPTGLLTPFHEKPNPLSPCPTPAMSLLPPTPPASSYGMTTDFSGIDEKLAIEQTITEEFGDDFVTQVYNYLSLGYPSMARPFDAELSKISMIPISELRQDDHLAKSRGYIRLGKDGNLKDREITEETCMRWRALRLYIQEWARQHPNMAEDEFLAGGRGTAVRKGSWAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCD1</accession>
    <name>A0A9W8YCD1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PTC7</name>
        <name evidence="3" type="ORF">N0V83_004193</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCD1"/>
    <dbReference type="OrthoDB" id="60843at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039123">
        <property type="entry name" value="PPTC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320:SF1">
        <property type="entry name" value="PROTEIN PHOSPHATASE PTC7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13672">
        <property type="entry name" value="PP2C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="127"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="437" mass="47102" checksum="46A2E1E10CA780AD" modified="2023-11-08" version="1">MSFAISNTAGLARLPPTSRRATPASLLLFALSASSLVAPSHSMRTAGPQSKRTLSPYAQPTCPLNRDTCPATIGSRSFHSTSHNRKPQFTYHVAASYSAKQDRFNAEQNLFTRPVYDPSESKTTDLRECKESIDKRQRAKSGQDAFFFSQVGNTSATTFGVADGVGGWVESGLDPADFSHGLCEYMACAARSWPHGSNTTSLHPKHLLQVAYDMVTDDANIEGGGSTACLAVAEADGNIEVANLGDSGFMHLGLNAVRHFTQPQTHAFNTPYQLSKTPQRMLVQMAVFGGPATLSDLPKESSVTHHKVRHGDVLVFATDGVWDNLSPQDALSIVSRHMVDLGAWVEKDGTIEVGQDLTKLVQADSARKADSSSLQAKVAVAIAKEAKETGLNTRRDGPFAKEVQKYYPGENWHGGKPDDIAAVVAVVLEEGFPRSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCH1</accession>
    <name>A0A9W8YCH1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002593</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCH1"/>
    <dbReference type="OrthoDB" id="4186885at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="190"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="324"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="362" mass="41025" checksum="58493FFEB11E1ED0" modified="2023-11-08" version="1">MSLLSQRQGNEPNDGINGNPSSLKKQHQHRRDPVERPVDDDATSRCSTLDGDTEELNYTDKTTKSLSAIENRGPDNETTSPAHVPGPLESQLAALMSKLIYIEQANPTVSIKPDEFQEMQARLKALEEEKKSWWKRHEAIWALRDEDVENNIKIRGMLAKCRRDLEATQALRNEDLINVQVVRSKLAEKTRELERSQAQTGRASPSRGRPGSFFERRGTSDLFTAAKIAALEQRALELERRNSDLVAQLQTSRAGMNIDDLNRLTAHEAWKDTVTDLESKLKAKDAEMARARPVSSTGVAMDWYRVEALLEEHANYREIVGGRLQALRSEKETLQRDLHAKENECQALELKVQILQRRASVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD14</accession>
    <name>A0A9W8YD14_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1748-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_002627</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD14"/>
    <dbReference type="OrthoDB" id="16824at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013726">
        <property type="entry name" value="Mitofissin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28075">
        <property type="entry name" value="CHROMOSOME 16, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28075:SF1">
        <property type="entry name" value="DUF1748-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08520">
        <property type="entry name" value="Mitofissin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4373888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="69" mass="7932" checksum="A74BF38A866032E1" modified="2023-11-08" version="1">MVVRTLRFLGRVAHYAFDAVLISAFLAGVRRSTGLTVKKWIDNYLWVGETLMDQSVAIFSSSSYFERKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDS3</accession>
    <name>A0A9W8YDS3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">COP9 signalosome complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_002121</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDS3"/>
    <dbReference type="OrthoDB" id="29061at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055089">
        <property type="entry name" value="COP9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050756">
        <property type="entry name" value="CSN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3/COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758:SF1">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22788">
        <property type="entry name" value="COP9_hel_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="8">
        <location>
            <begin position="247"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4375042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="501" mass="55358" checksum="11768AE35144FB0D" modified="2023-11-08" version="1">MSAELTSILLQFQPESPDLKHKRDYDQAARSFVSQLASISAAHWLKGAETAQDVLPVLNPAVNTIAYAFALRHRIAVLLDKRTLPDLLKPGGALWNNLVLFLETSDPVQLRYVGHEWRKLVDFTEHIARAIGSQQPGLAIAPIRSAMVRLDPTTGTFTSTHLHFVQLCCETRSYAAVEPILDNYIHTLPSKIPNVVREGLEYSVPCADVATSGDYIHQTSGHSDTKTSLVDVQEYYILGAMAYLGLRQFKKAQHFLEQVLIVPSSSVANGLMLEAYKKWVLVNCLVDGKSKPVPRTANGNAIKQVKSASKAYEALAEAYVQLGNLPKLKAQIRVGAELWAEDGNTGLVTELVNSQMKTYILRLSRTFSAIPVSNIANNVGGSADEIAQYLNDLIKSGQLNAGLEETDNSGVGVVLRFYLNPTQGPLAKTEKQQQQALFEQTLQTNILAEQVKNTDYRLTLAKEYLENLKRVNKRQAQTGDAMDTAWDDSVDAEEDIMVDLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFJ9</accession>
    <name>A0A9W8YFJ9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001366</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFJ9"/>
    <dbReference type="OrthoDB" id="5311681at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="422"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="427"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="646" mass="72529" checksum="78A0A47E1F3DEB67" modified="2023-11-08" version="1">MPGEILPHEESTAFHHDIVDFDPVTLLCVSAVQLPMAETPRSRRPPAPEDAAPKRRAALRIGGGPITAPIHSWFTSPARSHPNAAQAKELPLHLIQLILTHLDDVGDLARVTRTSRLFYYMTLPRLYEKVTLCSYSEIRYIDGRPEGYGGGSPFAMGLNTLVSRSFADYVQSFRVKGDWREHDVDDYKQGRVPDNSMVLQIVMRAALDKMKNLTAFAWELNTPPLHTVYQGLINKQPGLTSLTLRCQTRRIPRPTTIIPPLPNLVTLVVYDIDPLCYPDDISLLLVGSKKLDNLKLHWNPRMRGSGEESVNLMTMFGRCVAARHSLKLRRMAIYNLFTRFAGDGMNEIIDATAQTEITVLNSMGSTDPMTVFVDDAWRHSNKHPPPQNLKMLRTDNTDKYGAFMLGRFKGLERLYFVNNRPGREASKPSSAAATPTTPSTATPGMTNGNTSANGTPTVTEHQTRSIGGEYLAVIETNHRTLRHLLLSDKWQLSDDALFKICQSCPNLEQLAFSCQVPPFESLRQVLSLTPKLWAVRMLVRPNTDLAENIDSTEGAEMHTFVIATEFWRPEYRNIKYLGVGDKLVFKLGAVWFPPAKGKEKIPAGQENSMNARRAGPVRRVERVSRESVNHIEIWGMDSMEFDPSFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG36</accession>
    <name>A0A9W8YG36_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Pre-mRNA-splicing factor sap61</fullName>
            <ecNumber evidence="9">6.5.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">sap61</name>
        <name evidence="9" type="ORF">N0V83_000134</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3A3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG36"/>
    <dbReference type="OrthoDB" id="2160351at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051421">
        <property type="entry name" value="RNA_Proc_DNA_Dmg_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031774">
        <property type="entry name" value="SF3A3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024598">
        <property type="entry name" value="SF3a60/Prp9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786:SF2">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786">
        <property type="entry name" value="SPLICING FACTOR SF3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16837">
        <property type="entry name" value="SF3A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11931">
        <property type="entry name" value="SF3a60_Prp9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="8">
        <location>
            <begin position="404"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="326"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="282"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="327"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4377309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="499" mass="57953" checksum="CB5D426F2970DAA7" modified="2023-11-08" version="1">MLLEDQRQIHEDLERLEQAIAERLLEDPTHIRDRLARDHDIARFLEQIEAQSRRLLVLYEDADSRRAEEMRNLTLGDPMESFMKEVAAINDFHRRYPNEPVENLEKAYKKRSPEDQAQYLAAIDAMFTGEEGFGRYFDMTTLHETYLNLPVHQAARRLTYLQYLDMFDQFIPPQCNIPRQQKKSEAYFQYLKALQDYLESFMRRTKPLENLDKLFAAFDKEFNELWEKDEVLGWEKKAPSSAPVNGEAQGEGIWCSACSKGFSKETVYEAHLTGKKHKRAVQESQGASDNATAKTNGAATDIQRFKERAVAEREFRIQKLAAAMQTERADTKANVERKQGMTERERQQELEQLYSDKPDNGAQEEDNESDGEDKIYNPLKLPLAWDGKPIPFWLYKLHGLGVEFPCEICGNFVYMGRRAFDKHFNEPRHIHGLKCLGITNTTLFREITSIQEAESLWKKIQKDKKKEKTLAENVVEMEDSEGNVMPEKVYRDLAAAGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG47</accession>
    <name>A0A9W8YG47_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002339</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG47"/>
    <dbReference type="OrthoDB" id="4772757at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="349"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="423" mass="47750" checksum="DACFBEE0970950D2" modified="2023-11-08" version="1">MPQHTPQITTLEQSPSHGHEKHTFEVELTPEPDDDELPLQPSQSTVDIEEEKQRRLTIPRLGQNEADRRWYKEEFIRHNKNVTMKNFFAKSVDNALNALSRNYVRATRMHPWEILGRQPLAAYQGESNTALLQKHLDQFLEYRMNGLYGAPNDLPNLITRIVDFMASVSDGLSSTTREQWTQAAAHTVSRYCSALTFATEPTPEMKEDLAGIAVDVEALAAAVVLGDQDLYGRLRFPSELLDHAESCEKDEYRDVFLGAWGRFEHNSMSLSDRKSESLKEIISLFLEKGDFERDTINAGQLKNGSFDSIHKIRKRSLLHIAVWRSDLDLVHAVLDAGARPDGVRMEGGELTYPLISAARGGQMDVVRVLMARGADPEAGDRETRGKRTTLAGLKKGSEMYREISKAISEKHRPKKPWLAGDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG56</accession>
    <name>A0A9W8YG56_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tetrapyrrole biosynthesis uroporphyrinogen III synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002354</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG56"/>
    <dbReference type="OrthoDB" id="5595751at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004852">
        <property type="term" value="F:uroporphyrinogen-III synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006780">
        <property type="term" value="P:uroporphyrinogen III biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10090:FF:000011">
        <property type="entry name" value="Uroporphyrinogen-III synthase (UroS), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039793">
        <property type="entry name" value="UROS/Hem4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12390">
        <property type="entry name" value="UROPORPHYRINOGEN III SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12390:SF0">
        <property type="entry name" value="UROPORPHYRINOGEN-III SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="HemD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrapyrrole biosynthesis uroporphyrinogen III synthase" evidence="1">
        <location>
            <begin position="30"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="308" mass="33952" checksum="CFBD49FC3696E3A8" modified="2023-11-08" version="1">MAANSRGKIPILLLKTKSVPTDTYEELFLALDDHAYAPVFVPVLEHQFKQDALNEVQQNITNGGFVPKSQNGLATYGALIFTSQRAVEAFTAIVLSIRREGKHDIDDLLPGGLPLYVVGPATARGLRALNLRCPILGEQTGNGEALAAYILEHYNSIYPGAANPPILFLVGDKRRDIIPRTLQDHRLGSRTSKVDEVVIYETGEMQSFKAEFSSIWQDNLDQGCNRQWVVVFSPTGCRAMLESLRLLDANTGKAKPNARQGDIFIATIGPTTRDYLLHEFGFSPDVCAEKPSPEGIADGIRAFIERQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG84</accession>
    <name>A0A9W8YG84_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glucosidase 2 subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_001771</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG84"/>
    <dbReference type="OrthoDB" id="28322at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017177">
        <property type="term" value="C:glucosidase II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039794">
        <property type="entry name" value="Gtb1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036607">
        <property type="entry name" value="PRKCSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028146">
        <property type="entry name" value="PRKCSH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12630:SF1">
        <property type="entry name" value="GLUCOSIDASE 2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12630">
        <property type="entry name" value="N-LINKED OLIGOSACCHARIDE PROCESSING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12999">
        <property type="entry name" value="PRKCSH-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13015">
        <property type="entry name" value="PRKCSH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="MRH" evidence="7">
        <location>
            <begin position="406"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="348"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="139"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="375"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4376487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="536" mass="59580" checksum="F99393450D1C7734" modified="2023-11-08" version="1">MQLAKFYKDPETFACISNPSIKIPVARVNDDYCDCPDGSDEPGTSACSYLSPLSPSQPSGFKAGDLNTTPALPGFYCKNKGHQPSYIPFTNVNDGVCDYELCCDGSDEFEHVGGVKCEDQCAKIGKEWRKQDEARQKSLNAARQRRKELVAEAGRLRKEVEDRITTLKTQIDGQTLKVDSLTQSLAEIERQERGKVVKGAGKGGKITVLASLAKDRIQELADNVHRVRNERDAARGKVDELEAMLKRFKEEYNPNFNDEGVKRAVKAWEDYAAQERPTPNEALDRDLDEILKPDSDSAIKWEDFETVDETDVDVLYKFEEYLPVPVRDWVDTKLRDLRVLLIENGILADPHSSSSDTPESKVVTDAKSQLSSAQRELDADKSELNKHEDDLQKDYGPDSIFRALASTCIETDSGEYTYEHCFLSRTTQKPKKGGGHSNMGNFARIEIIAVDDGPLPPDGRGLGTGARIALKYEGGGHCWNGPNRSTLVVLGCADKEEIWKITEEEKCVYRMEVGTAAVCGVDVRMPEAQGEGHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGE6</accession>
    <name>A0A9W8YGE6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_000314</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGE6"/>
    <dbReference type="OrthoDB" id="691673at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901605">
        <property type="term" value="P:alpha-amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050859">
        <property type="entry name" value="Class-I_PLP-dep_aminotransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790:SF1">
        <property type="entry name" value="AROMATIC AMINO ACID AMINOTRANSFERASE, HYPOTHETICAL (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="129"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="70"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="598" mass="66446" checksum="64572A2458BB415A" modified="2023-11-08" version="1">MAPYTSDPGSFPDPIDLSHHLSRSTKAREASSVKRFYKYFSIPGIAQLAGGLPNDHYFPYDTLEAKVAHPNRWQPTPNKPVDPPPDDPPTAHLSKPAVSAAKDEEPPASRLVVPKDSSVPDPLRMIDLKSALQYGQAQGYPALYSFIRQFTRDNLHPFVPYKDGPEVILTCGSTDGFSKTLQALSNEWSAEHDPIDERPGLLCETYAYMNAIQSARPRGFNIAPVAIDDEGMVAKGPGGLEDVLENWDFSKGRRPHLMYTVTIGQNPTSGTLSVARRTEIYALCVKYDVVIVEDDPYWYLQYPSSSPASVPPRPTQSSGFEFLDSLIPSYLSLDYEGRVVRLDTFSKTVAPGCRLGWITAQPALVERILRITETSTQQPSGFVQSMIAELLMGPQGTKDPGRGGSADGGGWKVDGWVRWLEGLRGNYERRMQTMCDALETGKEVVKAGRRKSLGDEDDEWAVVEKTQIYSFVRPLGGMFVWVRFDFTSHPLAKVVPAARLSQALWVFWTLKPYLCLVAPGSMFAATQEIREQDSFNCYRLCFAACPVEDVEDVSKRFADGAQAFWRIKKKEKIDKLLEDEEVAGMDNVTGMAVLTGMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGK9</accession>
    <name>A0A9W8YGK9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001728</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGK9"/>
    <dbReference type="OrthoDB" id="5586090at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052646">
        <property type="entry name" value="Peroxisomal_PEX28-32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31679">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31679:SF2">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="161" mass="17681" checksum="C29BC90F27BCA5FD" modified="2023-11-08" version="1">MGQDGWGRYTRRRKWYRDAELVEATPSTDVTPVPTPKHNPIDDSASPLDDPNHLTKLSSNLSTTSTAASTTDPTMVDNSSALSDTDAASTKSKRSSWFKKKRRTKSTGAGSSVSNATIASSDVGTDISRRSDEDDVQSPLDRIARDDEWRLGDDIRQHLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH69</accession>
    <name>A0A9W8YH69_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002283</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH69"/>
    <dbReference type="OrthoDB" id="203440at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007146">
        <property type="entry name" value="Sas10/Utp3/C1D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237:SF9">
        <property type="entry name" value="NEUROGUIDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04000">
        <property type="entry name" value="Sas10_Utp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="359" mass="39867" checksum="B58B1C2B99247E0F" modified="2023-11-08" version="1">MAVDNSLESLLATLTSSIHSATEVLPNDDILPPKEGISLLDVKNELLLSYLQNLVFLILLKLRSRSSSDREEQEEGLHSHDEVVQKLIELRIYLEKGVRPLENRLKYNIDKILRTADDSARMSTQATLKSRVRRSKADEGSDSGVSEEGSVASAQTEEDEDEMSFAPNKAAVSRIKSRVSGDRAPESAKDGIYRPPKITPMAMPTPESREARRERRPGKSATLDEFVASELSSAPIAEPSIGSTITHGGRHTKSERERREEAERREYEESNFTRLAPESKKEQAKKRARQQDGGFGGEEWRSLGAGIDRIERLTQKKGGKLGTLEKSRKRPIGDGPRGSGSAAGDAFEKRRKVVSKYRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJR8</accession>
    <name>A0A9W8YJR8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cysteine synthase 1</fullName>
            <ecNumber evidence="5">2.5.1.47</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">O-acetylserine (thiol)-lyase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">O-acetylserine sulfhydrylase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">O-succinylserine sulfhydrylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CYS17</name>
        <name evidence="20" type="ORF">N0V83_000635</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the conversion of O-succinyl-L-serine into cysteine, the last step in the cysteine biosynthesis pathway. Can also use O-acetyl-L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-succinyl-L-serine + hydrogen sulfide = L-cysteine + succinate</text>
            <dbReference type="Rhea" id="RHEA:53816"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:136856"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJR8"/>
    <dbReference type="OrthoDB" id="10259545at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000011">
        <property type="entry name" value="Cysteine synthase (o-acetylserine)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007989">
        <property type="entry name" value="PRK10717.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="19">
        <location>
            <begin position="46"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00072087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00079147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00081847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ4377805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="378" mass="40765" checksum="F0367B3002BD12FB" modified="2023-11-08" version="1">MFRTAARSFAASAWRNAESVTQLEAAHQTAINISKAQGIGQRGFLDAIGKTPLIRLKKLSEETGCNVLGKAEFQNPGGSVKDRAALFVVEHAEKAGLLKPGGTVIEGTAGNTGIGLAHVCRSKGYKLVIYMPNTQSQGKIDLLRLLGAEVYPVPAVAFDNPENYNHQAKRHAERLGNAVWTNQFDNTANRQAHIETTGPEIWYQTEGKIDAFTCATGTGGSLAGVTRYLKEKSDGKVKSFLADPPGSVLHSYIQSGGKLAERAGGSITEGIGQGRVTDNLKPDIDLLDGSLHISDEKTIEMVYRCLDEEGLYLGASSCLNVVAAKEVAEKLGKGHTVVTLLCDGAYRYADRLFSKKWLEQKNLLGAIPQHLTKYIVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YKM6</accession>
    <name>A0A9W8YKM6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NADH-dependent flavin oxidoreductase</fullName>
            <ecNumber evidence="8">1.3.1.42</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">OYE32_1</name>
        <name evidence="8" type="ORF">N0V83_000862</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.42" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YKM6"/>
    <dbReference type="OrthoDB" id="72788at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016629">
        <property type="term" value="F:12-oxophytodienoate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02932">
        <property type="entry name" value="OYE_YqiM_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044152">
        <property type="entry name" value="YqjM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43303">
        <property type="entry name" value="NADPH DEHYDROGENASE C23G7.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43303:SF4">
        <property type="entry name" value="NADPH DEHYDROGENASE C23G7.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="7">
        <location>
            <begin position="60"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4378032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="425" mass="46750" checksum="C95B528C0EC8B1E7" modified="2023-11-08" version="1">MGAEANTDKPHSHHAIPKDIPEDDNLKNKAAPGVSYFTPLQDPPAGTAIGMLDGSDKIPKLFTPLKLRGLELQNRIALSPLCQYSAQEGHLSDWHLTHLGGILQRGPGLSIVEATAVQAEGRITPEDSGLWKDSQMEPHRRIVEFAHSQNQMIGIQLAHAGRKASTVAPWLSKGDIATKEMNGWPDNVYAPSAIAFNDHHCTPKEMTKEDIENFKTAFKQAIERALKIGFDTIEIHNAHGYLLHSFLSPASNKRTDQYGGSFENRVRLTLEVVDLARKTIPKDMPLILRISADDWLSEAGVEGWTVDQTVKLSEILADRGVDLLDVSSGGLHEKQHIHGGPGYQEPFAKAVKDKVGNKMHVGTVGSITNGKQANGYLEDDNLDMAFIGRMFQKNPGLVWTFADDLGVEGRWANQIRWGFGGRGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHE8</accession>
    <name>A0A9W9CHE8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Urea active transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_010374</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHE8"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11476">
        <property type="entry name" value="SLC5sbd_DUR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031155">
        <property type="entry name" value="DUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154:SF3">
        <property type="entry name" value="DUR32P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="284"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="352"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="427"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="492"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="616"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="648"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="532"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="533"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="544"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="577"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4362281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="689" mass="75874" checksum="2999F5C330A46FB7" modified="2023-11-08" version="1">MAVQLLTQGEGYGIIIGLSILFCLIIVAAVYIQKRYLSEDSDESEMFMVANRSVGTGLTASAVFSSWMWINESVFSAAYTYKWGIALPIWWASGLSFQIALMAVLGIVAKLRVPYAHTSLEIMKIRYGRYVHIVFIVLNLINNIFGCGSMILAGAQLVTGMTGMHVVAACILIPAGVVIYTVMGGLKATFLTDFLHTTVALILLIYFSLAVLTNEHIGGVSGLYEKVKAADVYIPGNYEGSLLTMKSRSSVLFGLVLKFGNLALVLMDTAFWQKSFASDVKATVPAYALVSVVILAVPWCTGTIIGLSARAIESTPIWFDYPNRLTLTQVNSGMVMPYVLRSLLGQGATTGLLVLVFMAITSTISSSVIAVSSIISLDFFRTYINPKASDRKTLSVSHWGVVFHGCFMAGFAIMLEYAGATNNWSTYFRPIIACPGILPIILSLLWSRQTKLAALLSPLLGLASGVTTWLCLSWKWAGVINITTTQQQLPGLYAACVSFFSPALFSVIISLVKPEKFDWREFLRIDLIEDESASSNSSSPSSLSDKEISAKHTSDPTRASNPEPILPSISAEEKGRRPSVSKHSPHIHNSQVPLDEVVHPFDEETLRHVRRWFKIASVYFVVNVLATIVLWPMPLYRDYIFTRTFFDGWVTMAIIWHFAAMFAVIVYPIFDGRHAIAKAVRGMVGEFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHH4</accession>
    <name>A0A9W9CHH4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small nuclear ribonucleoprotein Sm D3</fullName>
            <shortName evidence="9">Sm-D3</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">snRNP core protein D3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SMD3</name>
        <name evidence="11" type="ORF">N0V83_010434</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the snRNP core protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHH4"/>
    <dbReference type="OrthoDB" id="6425924at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01721">
        <property type="entry name" value="Sm_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000002">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034099">
        <property type="entry name" value="SmD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9 11" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="7"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4362341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="116" mass="12760" checksum="777F747DDC5C2FBC" modified="2023-11-08" version="1">MTSTIGIPIKLLNEAAGHIVTLEITSGEVYRGKLIEAEDNMNVQLKDITVTARDGRVSHLEQVYIRGSHVRYFIVPDMLRNAPMFRSRGTRGRGVGLARGRATVIRARASTRGGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHL3</accession>
    <name>A0A9W9CHL3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010008</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHL3"/>
    <dbReference type="OrthoDB" id="10255963at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040773377" evidence="2">
        <location>
            <begin position="19"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="196" mass="21780" checksum="4F0415616B1BD8AA" modified="2023-11-08" version="1">MWKSLTVLGLGAFLGVNANNGIPEQWFTDDQGSQAAQAGSPYVLMSLSSALRTYYGVYFKKDVGHKSDWEYAIMKWTPDGSGNFVRDSVSLEVDGKRGGGSYSDIPSTFDSSDDQYEDGNQGRDHPKLYFGKHHHAVHWDMSGNFRSTCGIGTDDFRANDFQFWSSSNLRHIDNVDPNWTYGKATNPHVTVGGMCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIF7</accession>
    <name>A0A9W9CIF7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRISE-like Rossmann-fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009596</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIF7"/>
    <dbReference type="OrthoDB" id="1731983at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055222">
        <property type="entry name" value="PRISE-like_Rossmann-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32487">
        <property type="entry name" value="3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32487:SF4">
        <property type="entry name" value="SIRQ PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22917">
        <property type="entry name" value="PRISE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRISE-like Rossmann-fold" evidence="1">
        <location>
            <begin position="64"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="194" mass="22247" checksum="F92CFDECFF19EC33" modified="2023-11-08" version="1">MADQLPVDYLKTVPYLHYRAMLTEESANKDWSWSEVVPDAIIGFTPSGSGFSLAGHWAGYHQWVAIFASLNPENFKKRIFNVADSATPESMRERWAQNASFFGLKGVPPLPAASASDPKPSDFIKQHEEEWKKVGIKGVDIWNAAQLDSYGYWLTFDRHLSLQRLRDAGFDEENRPEEGWWETFKMFRRAGMIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ76</accession>
    <name>A0A9W9CJ76_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008752</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ76"/>
    <dbReference type="OrthoDB" id="3684516at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="150" mass="16911" checksum="D50D6F2603E97A22" modified="2023-11-08" version="1">MAPNDSRHLLPMNSIVFNSQQSPRPPRFTISYDPTLANLHEAQDTTITALPQPNPITEKPPTAHKRSIWSRMVNKLKIVFRRHKKEKSASIGSLTEYQHVVTSGPEPLRSVSPPAATVAGAKRDTEGTAVEEDNESDWEDVEETRLFRRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJB1</accession>
    <name>A0A9W9CJB1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alanine--glyoxylate transaminase</fullName>
            <ecNumber evidence="3">2.6.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_008842</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.44" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJB1"/>
    <dbReference type="OrthoDB" id="7403325at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008453">
        <property type="term" value="F:alanine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004760">
        <property type="term" value="F:L-serine-pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019265">
        <property type="term" value="P:glycine biosynthetic process, by transamination of glyoxylate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000049">
        <property type="entry name" value="Alanine-glyoxylate aminotransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000027">
        <property type="entry name" value="Serine--pyruvate aminotransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152:SF24">
        <property type="entry name" value="ALANINE--GLYOXYLATE AMINOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152">
        <property type="entry name" value="AMINOTRANSFERASE CLASS V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="9">
        <location>
            <begin position="97"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4365224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="454" mass="49904" checksum="E9B287DA004827EF" modified="2023-11-08" version="1">MTSRASSRLNQISKHLDSAKTFLELNTPFSTERSAHLEDEHGNRIKREKIKEVKKSVQEVKKITPEVPKKMSSQPSHPALLIPGPIEFDDAVLTSMSHYSESHVGQPFVNTFSEVLQNLRKLFQTTDPASQPFVISGSGTLGWDQVAANLVEPGDEVLVLHTGYFADSFADCFETYGVKATQLKAPIGDRPQLDEVEKALKEKSYKMLTVTHVDTSTGVLSEIKALSELVHRVSPETLVVVDGVCSVGSEEIRFDDWKLDVVLTASQKGIGCPAGLSILMVSGRAIERFKARKTRPTSYFGSWKNWLPIMQNYEAKKASYFATPSPQLIHALDTALKQILSRPIDDYFAAHKAASQKVKKAVADLGLKQLASKPENQATGMTAIYLPEGITPPEVLPNLLKKGVIFAGGLHKEIATKYIRFGHMGVSVTDTSRPDITKAIDSLKESLVEAGYKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJD3</accession>
    <name>A0A9W9CJD3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">White collar 1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">N0V83_008251</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJD3"/>
    <dbReference type="OrthoDB" id="447251at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000065">
        <property type="entry name" value="GATA transcription factor LreA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000064">
        <property type="entry name" value="Vivid PAS protein VVD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000063">
        <property type="entry name" value="White collar 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000087">
        <property type="entry name" value="White collar 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47429:SF7">
        <property type="entry name" value="GATA-FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47429">
        <property type="entry name" value="PROTEIN TWIN LOV 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0157">Chromophore</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PAS" evidence="17">
        <location>
            <begin position="388"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="17">
        <location>
            <begin position="563"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="GATA-type" evidence="18">
        <location>
            <begin position="925"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="220"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="794"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="220"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="255"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="274"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="794"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="814"/>
            <end position="842"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ4365631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1150" mass="127832" checksum="3CE2B05C2B086D54" modified="2023-11-08" version="1">MNGYPYPSRPFPRAANGSNMMGTSGDGMNTYNMVEGQSLDSIVAQNDKENRRRSMPVYARPAPMQADSPDTRRLSMMNFGDPNGASVDNFQFDMSASSEMEGMMPTNTAFPGPTSNMQGDRAPLPADDLVINTQFQAQNSPFPTIAAPGSAYASPMHQKGSLDMDMSPYPSGMSMSLDMDDSLAMVPNEMNMFPTSQYNAPMLGSPMNQEFVRPLPTVLQDSNVPSIQSTDRFQKPSSSNTPEALSGGSGFPSRAGSQDQNSFRSNSRPESEQRSTPSVPTQMSLNSLKNQQPIAQNPAQNMSKETINKQLSDFKLPWAPPAGGFPSTMHSNPHMKTQFKNAYSSTGFDMLGVLMRVATRPDPQIDIGSVDLSCAFVVCDAELDDVPIVYCSENFERLTGYTRHMILGRNCRFLQSPDGKVEAGIKRNYVDDDSVLYLKQMVENMAEAQISLINYRRGGQPFMNLLTMIPVAWEPGGNTKYFVGFQVDLVEQPGAMTNKNSDGSYRVNYQRGMSIPSYVFTDHHKPQGEQGQTISKDEVSQVLATYSGSGDSEITRRIWDKVLLENTDDVVHVLSLKGLFLYLSPASNRILEYDPSELVGTALSSVCHPSDIVPVTRELKETTNGTSVNVVFRIRRKKSGYMWFEGHGSLHTEQGKGRKCIILVGRERPVYTLSKAIVRESGGVGDNELWTKMSTSGMFLFVSSNIRQLLDKQPEDLVGTSIQSLMRQESKVNFGRVLELARSGRKGEVKHEMINKRGQVLQAFTTIYPGDAVEGQKPTFVVGQTRLLKYSRNSAASRPSIYTSNNKERHSGESQHTLQSQYSGGSHYTGSLTGSTTPQTTATNPLFVTTEEQASTFAGHNGLQLGHQDQSLASDDNVFDELKTTRSTSWQYELRQMEKRNRYLAEEVQSLLAAKKKRKRRKGAGQMQKDCANCHTRTTPEWRRGPSGNRDLCNSCGLRWAKQDAKEEEVMDENNRITITVCGDGGCGRKELNYTTFGEESMDVRDSYSVTRTVDGTPYYLMLTDTAGQEEYRGLWAASNLQSDAFLLVYDITSGNSLEALDYFMEMIDMETDNRIDNGKVEPIKIVAGNKCDLQGQRKVSAREGLEWARARKCGFMETSAREMVNIEETFACKWEDTRLGRNQSQNQDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJD6</accession>
    <name>A0A9W9CJD6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mid2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008918</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJD6"/>
    <dbReference type="OrthoDB" id="5425782at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040992768" description="Mid2 domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="279" mass="28558" checksum="D3C621D2EDE7E1A0" modified="2023-11-08" version="1">MLRKLWLFAALFAMAALSSAFSLDDLHIDGIEEYLHHGEKRQASGTGNTPSSAAPPTSSAQQVSSAPESTPTPTPTQDPTSDPVTSNEPASSTPAPAPSSANNQPTSQQTSAAAPVSSNKPVTSTRTTAVIRTTDLVSTSLQEFITTYTTISDGQAVTQTSTGTSSIVTTTGQAVVTDPPTSQENSGGSDKGLSQSSKNIIGGVVGGIGGALLLAGIGLVFWRMKKRQHKVSADDDDLMANTGAALGDKPSIGSGSSPFQSNLEQYHNPGGRPNAAANF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJH8</accession>
    <name>A0A9W9CJH8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RTA1-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_007510</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJH8"/>
    <dbReference type="OrthoDB" id="4521223at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007568">
        <property type="entry name" value="RTA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465:SF9">
        <property type="entry name" value="SPHINGOID LONG-CHAIN BASE TRANSPORTER RSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04479">
        <property type="entry name" value="RTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4366980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="358" mass="39676" checksum="31D2CAC81AE0C761" modified="2023-11-08" version="1">MDTILHLAVRALDQNGIDPNNLDVNTTDPLKIAMSRKYCKVGKCPESWQTIAYRPTIPGNVIYMLCFFALFGGQMWFGIRNKTWTYMGTMCVGIFGEIIGYIGRIMLNVNPFIMNNFLVNLIPLTIAPALLTAGIYLCVGRVIVAVGSENSRLKPKMYTYIFVGCDLLALVLQAIGGGMAATAKDHKGSRQGVNIMIAGLISQVITMTLFLAIWGDFAVRTRRAKLSGSFTRAQPSLYENLRSTRNFTYFQWSLFVATVLIFVRCIYRVAELWNGFSGHLANDQVTFMIFEGPLIILAVSAMTAYHPGRIFGNLWVPVGKGVTSMGKLAEGSDSSVRLTETQWNSTAYQRVEQPGNVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJN5</accession>
    <name>A0A9W9CJN5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008448</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJN5"/>
    <dbReference type="OrthoDB" id="1046782at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359:SF37">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="402" mass="45661" checksum="AD0DF29CE7696D05" modified="2023-11-08" version="1">MNPPLASQVALESGNDIESSPPESSLPQTLPSERKGTIKRLSNVIPRLVPFVFSQEDSTKRHRKTRSENVLSIPHVKQPNGSGLELRKTRSTVDGAQSDPTEEQQMNQDDHALDVQDKARVTWLIQQCHGIAEALYRIHRYNTTSATSIPHFMTLPKPEPSINHGNRQSNGAADKRSPRNLFGRHGDIKPANILWFPDKNLPRRYGLLKIADFGTARFTDDKRAAVQDRDVMPRSETYMAPEWRLPDSELSIQCDIWALGCIFLEFLCWYFEDWQLVQEFARQRTYGYESDCFFTIEMTGKTRSISAKVKEPVSEMLEELRKRSRDDSNNCFLGILDTIQNDMLVVQGDNLDSVDETNETESSPASGFLKVNHEARRRRKSSGNIARDLGSIVQALGQPQME</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJR0</accession>
    <name>A0A9W9CJR0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007979</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJR0"/>
    <dbReference type="OrthoDB" id="3796275at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="345" mass="38287" checksum="47FAD84934206D85" modified="2023-11-08" version="1">MSKTWKHFAAFEHTQIGEERDLQGRFLHNAVDMVLATIRTLCDSDHGSCSEIKRLKHFLPNELSAGSAKIVPVRERARGEPDIVFKIAGIGNQQTVGVIGELKFFVTCDVRTQIEKGQKEPETRDSQARGSLRHMFGQVARDMRNRGVRFGFFSTYSETVFLKIILPPGGKPGLLYSEPIKHTTTVSLDASTNMLSNVSIRLGILYLLHRASDAEVSRWQFNTTDIDATKWTASKPNDELGTTATSYNTPAGRSGIPQPTTTTGTPRRHTLAPVGDDDLPDTEDTFNTRELSENLPTTIVFFSMLGLSLMDNREALQDDLILEGVRPYVKVSLINDDDGAGDDQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL22</accession>
    <name>A0A9W9CL22_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_006382</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL22"/>
    <dbReference type="OrthoDB" id="269227at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF115">
        <property type="entry name" value="DEHYDROGENASE XPTC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00623">
        <property type="entry name" value="GMC_OXRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040952832" description="Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein" evidence="5">
        <location>
            <begin position="23"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="6">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="7">
        <location>
            <begin position="287"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="540"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="583"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="121"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="604" mass="65140" checksum="6D2C748991EFD810" modified="2023-11-08" version="1">MRGLTFSFILTTGLGLLHGASGHGGCHVEDSTFDFVIVGGGTAGLALATQLSRALPKECVLVIEAGPDGRDEPKIYVPGLRGSTLGGVYDWVLPTVPQAAANGRSIVHNRGKVLGGSSALNLLVWNRATVKEYNAWEELGNPGWGWKNMYPAMLKAENFQREHGIAQYGRDGVGYGGPIQTALIEDPPPHVQAGIPTLENLGLSNNLESLNGTSIGAEYQPAMHRISNHTRSYSVDYLPRAGDNLVVLFNSTVHKVNLDKKGSKATGVTLIGGKEIKASKEVILSGGSLLSPKILELSGIGQKSVLSDAGVQQLVNLPGVGENLQDHLRIQTTYELKPSILGLDILRYNSTLAAIQLDLWKRGLTSLYQYAGSSYGFLKWKQAIDNDDKLIKLAQQSANKSNPIDQKKLALLTDENSGAPDLEVVFGDGYIGTKGYPANGTTGYGKSYATLLAGVQHPFARGYVHINGSDPASKPLIDPKYLSTAYDLEALIQAVKYLRKMATTEPFKSVWVSEFDPGMDTDTDAEWETYVRNNVFTFFHPLGTCAMLPKKDGGVVDPKLRVYGVENLRVVDASVIPMIVSAHIQTAIYGIAERAADIIASKYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL75</accession>
    <name>A0A9W9CL75_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Importin-alpha export receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CSE1</name>
        <name evidence="9" type="ORF">N0V83_005371</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the XPO2/CSE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL75"/>
    <dbReference type="OrthoDB" id="3268246at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000057">
        <property type="entry name" value="Exportin-2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005043">
        <property type="entry name" value="XPO2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013713">
        <property type="entry name" value="XPO2_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF8">
        <property type="entry name" value="EXPORTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03378">
        <property type="entry name" value="CAS_CSE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08506">
        <property type="entry name" value="Cse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="8">
        <location>
            <begin position="24"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4369609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="959" mass="108352" checksum="6311FBC08CD7D32E" modified="2023-11-08" version="1">MATDIATLSQLLQASLDPRHNKQAEAAIAQEQTKPGFSLSLLHIVASDSSPQTTRLSAALYFKNFIKRNWVDEDGNYKLPQDEVVAIKRELIGLMVSVPANLQSQLGEAISAIADSDFWQRWDTLVDDLISRLTPDNTTVNNGVLQVAHSIFKRWRPLFRSDDLFTEINHVLSKFSAPFLQLLESTDALITNSQGNPAALKGAFTTLDLLVKLFYDLSCQDLPPDFEDHISSIAGLLHKYLIYDNASLHTSDETESGPQEYVRAGIFEVLMLYIQKYEDVFGPQLGQFIDSTWNFLMSVGLETKYDILVSKALQFLTAVASTQHADAFNNEAVLRQVIEKVILPNLTLRESDVELFEDEPIEFIRRDLEGSDNDTRRRAATNFLRQLMTKFEGLVTTTSQTYIHDYLKNYTEDTENNWKSKDTAVYLFTAIAAKGTATAAQGIISVNDNVNILEFFQSHIAADLQAQDAFPILKVDAIKFLYVFRSQLSPDLWRAAFPLLVNQLGNDNYVIHTYAAIAVERALFMTDSNRQPIIPRSDVVGSSKDLLTHLFKLITKNSAPEKIQENEFLMKCAMRVLIFIRDGVLPICESILQHFINIVKVIRHNPSNPRFQYYLFEGIGALIRFGAPQHPQFFEERLYEPFAACLSEGVEEFSPYLFQLFSALLEANPSGELSDLYRSLFEIIIQGAVWEQRGNVPALARLLSAMIARDSRQIVDNHKVEPILGIFQKLVTSKAHETYSFELIEAVVAHIPAEALQPYFVTILQLMLTRLSNMKTENFQQRFIAFYHFVSARKDKGLGADFFISVTDQIQHDIFKPIYLTVILPETQKLARPTDRKTAVVSFTKTLGDSQAFVDRYPKGWTLTTQRLIELLVNPPVPTAADDIIPDADVDETGFGVGFTQLNTCKKAPRDPFPEITDVKAWVGQYLKDANTRHNGRIVNIVQERLDPVSKQALAEYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLA3</accession>
    <name>A0A9W9CLA3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycosyl hydrolase family 13 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_006758</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLA3"/>
    <dbReference type="OrthoDB" id="1740265at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004556">
        <property type="term" value="F:alpha-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033934">
        <property type="term" value="F:glucan 1,4-alpha-maltotriohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004574">
        <property type="term" value="F:oligo-1,6-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004575">
        <property type="term" value="F:sucrose alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000025">
        <property type="term" value="P:maltose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005987">
        <property type="term" value="P:sucrose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11333">
        <property type="entry name" value="AmyAc_SI_OligoGlu_DGase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.400.10:FF:000001">
        <property type="entry name" value="Maltase A3, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000064">
        <property type="entry name" value="Oligo-1,6-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000087">
        <property type="entry name" value="Oligo-1,6-glucosidase IMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="entry name" value="Oligo-1,6-glucosidase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF232">
        <property type="entry name" value="GLYCOSYL HYDROLASE FAMILY 13 CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0462">Maltose metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="6">
        <location>
            <begin position="28"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4368401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="589" mass="68093" checksum="AF9D936A645782BE" modified="2023-11-08" version="1">MSGVARTQKDMYVGTDKHRWWKEAVVYQIYPASFLDSNGDGWGDVPGITQKLDYLKDLGVDVIWVSPIYKSPQADMGYDIADYEDIDPSYGTLADVDNLIKEVKKRDMKLVMDLVVNHTSDEHAWFLDSRSSKGSAKRDWYIWKPAKYDADGNRQPPNNWAQILGEANSAWTWDEKTQEYYLSLFTPEQPDLNWENPAVREAVHNILRFWLDRGASGFRMDVINLISKVQTFPDADVVVKDNKYQPGDKYYANGPRLHEWLKELNRNVLSKYDTITVGEMPFVRDEDEIMRVVGAESEELNMIFSFDLVDIDNVPGDFKFTLHPWDASDLKRILNRLQRLMLERDGWNSLFVENHDQPRSISRYTDDSDEWRDYGAKLLCLMQTTLAGTLYVYQGEEIGMRNVPTSWGPEEYKDIESINFFKKYRDLYPNDPEKQALAAKILQRKARDSARTPMQWSSDPQAGFTTTSKPWMRVNDDYTTINAAAQLSNPNPAPGTLSVHAFWKRALANRKQEKDVFVYGDFEMLDMEDKKVVAFRRWSKEKAFVTVLNLSGEKVVWEGLGKLKVGKWVAGNYDERELEGRAVGEVLWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLI0</accession>
    <name>A0A9W9CLI0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA (cytosine-5-)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_006293</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLI0"/>
    <dbReference type="OrthoDB" id="414133at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003886">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044027">
        <property type="term" value="P:negative regulation of gene expression via chromosomal CpG island methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.120.10">
        <property type="entry name" value="DNA Methylase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629">
        <property type="entry name" value="CYTOSINE-SPECIFIC METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629:SF52">
        <property type="entry name" value="DNA (CYTOSINE-5)-METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00105">
        <property type="entry name" value="C5METTRFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="24"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="251"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="689"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="253"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="278"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4369208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="743" mass="85200" checksum="6AACBBD21A211C74" modified="2023-11-08" version="1">MSGSSLEDAISFIDDEADLAAYYEQDEVDVQRYDSDDATDEDEDDSVLGSDDDEDITIYEKAPPAFEPSPETFELPSLSHKCGFIRPGSVIELRDHSGRKSNHLLSGDFFLVQSIVEDVQTEEVVLKGYRMRRVDYLQPLFNRKLNDLFMLIEVKEGDSRPRFIQALSDIDPQEVIRVRRCVFTHLDDQRLSPAHVNRQIKPGLRTENEKREWIFHHGALVCRWVYVVEHDHNKKSYGGEARRMFQREVANFSQSNDSSQPSRPSQPSSASSSSTSQGTQRQKCPQTHSKSANRFQHFPEKCDQYTLGDGYCGAGGVSEGARQAGFRIKWGLEKDAAAMEAYKANFPGTMHLEMDAHDFMDMATRSLHRCDHLHMSCPCCFWSQAHTNEGKNDEANIMTLFTVEAWLHKLKPRTFSLEQADGLIKLAKHRMFFRILLNGILNRGYHVRWKVQDQAWFGIAQHRRRLIFVGAKIGTALPPFPNPIHGPKGTGLKRYVTVEDALRPLERQAHSLRHDPYHKPDLERRVDGEKYDPHVNLARCITTSGGDNVHYSGARAYTCRELSQLQGLPTTFHFAGSVSEARKQCGNVWPAKSNKIYFQLWAAHMEAFDNGFINAEDEVLDLFDFLEKKGIMIPKPEPIEIDLFNTPPPVRRSSGVPEYRYLHHIEKTIEPRIPLQLWSKRKALDPLPQQRRRRNNALLPVNFSRSRRRSTSEDRRGVTVIPRKKRRNWSSDDDGIITVDDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM13</accession>
    <name>A0A9W9CM13_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable double zinc ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004909</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM13"/>
    <dbReference type="OrthoDB" id="3793432at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058253">
        <property type="entry name" value="Zn_ribbon_double"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26652">
        <property type="entry name" value="Zn_ribbon_double"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Probable double zinc ribbon" evidence="2">
        <location>
            <begin position="69"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="317" mass="35636" checksum="863E51E4D94D41A5" modified="2023-11-08" version="1">MSNSTKSFGKVAKALTAFLPHGQKKTFNQAFQEKLDKARPAEKAWTREDYMSAGVDVANPCLRTDASDGLWVCCCGHENTLVHYQGAFPFKYLKCGRCDHILCPHCRTSEILTPVQAHILHVELDEQSSARREEVRFCRLCRACGLMHRASMDGCHVRFSEDKCRCGEAFMKEGSYFYIGDVFAWRCDPAGRAVELSLDQKLAASERYLANQKTVPEKVKSSATELYPPPGLPRVQTSYFSLAQEPRPHQLPRGATVTDPSRPVRRGAVRRPRETSPSGGNLVRLENGPWTPDKLGATMVRRPYAYREGHELRAVNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMD5</accession>
    <name>A0A9W9CMD5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dolichol kinase</fullName>
            <ecNumber evidence="3">2.7.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SEC59</name>
        <name evidence="12" type="ORF">N0V83_005303</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polyprenol kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.108" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMD5"/>
    <dbReference type="OrthoDB" id="377083at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004168">
        <property type="term" value="F:dolichol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043048">
        <property type="term" value="P:dolichyl monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032974">
        <property type="entry name" value="Polypren_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13205:SF15">
        <property type="entry name" value="DOLICHOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13205">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="487"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="582"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="628"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="681"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="734"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="773"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="109"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="374"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4370781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="861" mass="95561" checksum="0E23A6C003B784D2" modified="2023-11-08" version="1">MQQDPDPPDPQADALAPEDVETLELFRRSPHPYLRHRDEIRKQSPYRSTNPRRRSRTPLRPSNRTASDEDRRKRRKRSQSPSESGTEADDEGYNFVKALPAPPLRPHKGLRDTRGSGREEWASPLLTPTQIDDEGRKLSEGYFNDTKKGPRRGDAALTDEEATVARQKYRKRRRNELVRRTTETALLAGISVLAVQGCGCWSQLLSWHRGYSNLHWLVSILPLIAAENTDFPSKLLAMKPYKLKLPPPDKGLDPELLVTLFPLHQALLPPLYYLSTTSLLPAELHLLSIGLINMLLFSDSPQATILKILMWIGGLGVFVFCGKVLKWGVALARIPRWRLRRAGQVIRARQTFLQLLNEGLRPKKTKTPASGDITDSDADEDEPLLQNPLQKTKSLKVNILDSMRSAKLLANGHEPQSAVEAKKTGFEGSDGRVEDLDNVRRMRRNTLPNLAADDHTSNHQYTQRKRSRSIAQAYLSLTPTQATIRKWLYAGYFYLIVVLMILGPIRYAIGKYALHGHEPFGWAVGYLFGNIPRVRWEVINWNLDAWIPLPPLLDTESLTNTTEHLPRAEFYRQTLLGSANTRLLLCIYCGTTIITGLISVFSLSAVAEVDTRRKVFHGTMVAMLLPTIYIDPCFVTLALALVLAIFILLDLIRASQLPPLSKPIARFLTPYVDGRDLRGPVVVSHIFLLIGCAIPLWLSLAGVQRVGGGSGGEQDGLEQEQEPWQGWDVQRRDVSMVAGVVCVGMGDAAASLIGRRYGRRKWPWAGGKSLEGSLAFAVAVTVGLVFGKAWLHLGWEGGRGHHAGVGGLRSLPELLVEGVGVVAKAALCAAGASLNEAVLTGGNDNVIVPVILWVLVRGVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMH4</accession>
    <name>A0A9W9CMH4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMH4"/>
    <dbReference type="OrthoDB" id="4456959at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="157" mass="17687" checksum="BD62F2A3E5CECD26" modified="2023-11-08" version="1">MADQPEWEASYAGCRADVGRLDQLFATECVYQPKQKPGLKPGAVEALTRRVAFLEQILLDNAGNIRPQYLHDEVAQNDRGSHHENGGEVVLQVQENFHYSDTPSDYRQDANGSTNTDSQPHRQEKPSTNPLKRKLDEPLLGDWLVTLIMVKLKKPGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNJ3</accession>
    <name>A0A9W9CNJ3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_003309</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNJ3"/>
    <dbReference type="OrthoDB" id="1661054at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007255">
        <property type="entry name" value="COG8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21311">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21311:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04124">
        <property type="entry name" value="Dor1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4373018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="489" mass="53512" checksum="C1FD77F618B0DFD3" modified="2023-11-08" version="1">MADPLYELLTPYFDAQDAKVRPSANDAVTNAYLAHLTTLPLSALTSTEQQLLSQSSQSILRSLQALSKRSHKPIISSTDHLLHLRKTLPQLGQDAATVQAELPNLETAAQKFSDKYSRGTENAILDRRRKAMLLSRNVDRVSDILDLPTLLSSAISSSTAATQATMSAASSNANYASALDLHAHIKRLHTLYPNSQLISALTAQAEQEMKAMTVNLISSLQSQGIKLAGAMRTIGWLRRVAPELDESWSTKQTGIGSGEGTLGAMFLVCRLASLELMLGALDPLRELADHETEKRLSKTTRDNAGWAVGQQTEKYLKKYLEIFREQSFAIISMYKSIFPSALPAPGSEDSAPPVKPVAESNPLQPIPSALATFPLHLVEMLFDTLRAYLPNVQDRSARDSLLTQVLYCAGSLGRLGGDFSMMIALLEEDLRAEIEDTGDEETQEVEVEEEWVEVMKKHRVQASRLELLASGVSTGRTSSPVDRVASPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNV8</accession>
    <name>A0A9W9CNV8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNV8"/>
    <dbReference type="OrthoDB" id="5428863at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112:SF1">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="215"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="680" mass="78402" checksum="BC46F2DD3B1F5217" modified="2023-11-08" version="1">MRSREPSNNLICNECRQINFDQVLGLDPAEHSDLGFCIQKVGKRFRQRTPTNCQLCQILFDHCCFDYKGSEIKVDDGQDELRVFHFMSFNDAIDPLKTERGRDTRRTHPLWGLAVVPSSWHLYAQELDDPGLLLRSHFQEHGSPVIYHDDSPAPETFAVQRISTRFDLSLVTKWLQFCSQYHNKHCPKSNHSVDRLRLIDCESLDVKTAHPEDIYVALSYVWGSPDRDNAPKGGGNVIDDAQLPSILPAVISDAIKVTRLLGFRYLWIDKFCIDQDNPGIKHDQIRQMGRIYEDADLTIIAAAGLDENHGLPGVGSTPRKAQPAMEIGNVTILSSMQHPHSSIRSSKWFTRGWTFQEAMLSNRRLVFTDEQVYFECNAMNCSESVYTPLEKLHVKDKSKQRDILRAGVFGRNGKEAFGGLNINTLHFERVFPRYKAVIEEYSARDLRYDMDSMNAIIGVTRKFESLRHPYSHVWGIPFPVSVKPEVALDHFVSSLAWFHTQDCWNTNTKPRRRVGFPSWSWCGWAGQISFPLLVHNNLWAISFQDKCGMIHDFAETFQGGRIEELQELLLQARVLPSSAFSYNPASNEWHIFNRFAKLNLSQGRTSSCDGFWKELRAGRRWKCILIGSMLDTAHIMILESSHKDFWYRAGIFQIFDYRECEKIWVEQKLKQKKVATFRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP29</accession>
    <name>A0A9W9CP29_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003221</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP29"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="70"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="245" mass="27495" checksum="7508A2366892C3DD" modified="2023-11-08" version="1">MTPSSKVSSSQHPQPPDSAVQAHLTTPIQLFALNTMSEPTLPFPQTGDAAVDFINNHTVVDNDVPLLNECPICLDNFTSEPCLRITEIEGCNHQIGLTCLREILRSHPGEEKRCPLCRTVWISATAAPTRTVPRSEESQRNFASLFEDLDGIGENIANRMATGMNRQIADATTSRNVPRRNMHGYGSLYARDQRNTSMQNPILLDSDSETEDYETRLKNYESLSRDIETIRARARNTQLSRSQRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPE6</accession>
    <name>A0A9W9CPE6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPE6"/>
    <dbReference type="OrthoDB" id="5043642at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021848">
        <property type="entry name" value="HODM_asu-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11927">
        <property type="entry name" value="HODM_asu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="396" mass="45522" checksum="C13F8A5EE89110BF" modified="2023-11-08" version="1">MKRLCKGWYTFATQLAQLSDPINPSLYLNMANHDGPDARPMYVLLVLAVALCAFFFWVGTVCVRLWFYKGALGPEPEKFIILRKDLKQRFRNSMPDGDCEDLQQNPGSDSSTSHDRRVTVFPHVASPALVSPDTAQSTALDSRRFSTLPTINIKEVEDLNKFEDPNIRQFVAEEVRISRLPAFVQDDCGDGSDFPEIPVALEPIEEHVGNTLNSSPLQRTEVYLRLGLKKVDRSEWLTVDNTYIEYHEARKALLRTREVECIQVKHEGETACEELLHEVVEFLTNTYPNHFSVRTKDRRKHIRNELTQEEYSLVRPYDHHPLDVCARLAMEDFQVLIRGDFTMQWYLQASATLFPAGWNMRAHIGKPLDSILSNRNEPMPLWEDIPSTAKVWGRCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPF6</accession>
    <name>A0A9W9CPF6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MFS transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003599</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPF6"/>
    <dbReference type="OrthoDB" id="330047at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052599">
        <property type="entry name" value="SLC43A_AATransporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20772:SF4">
        <property type="entry name" value="HYPOTHETICAL AMINO ACID TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20772">
        <property type="entry name" value="PROTEIN FMP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="392"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="434"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="461"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="510"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="550"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="623" mass="68775" checksum="844EEFCCE594C3FB" modified="2023-11-08" version="1">MSLIQHVTQQEGTDREEALVDLFLPPVDRQISRQEASERRFSLESAISGHAGFLGLPGKQVRRVISYDAIKKPDEPVAGEEYGGLTPYKVSTAKRIGQVIVTVLACWFASGIVFGFAALKPILIKEGVFSDLCTKEELDAGVEVCFDQDLRLNFMFALASTTANVSALPVGTLLDRYGPRICFLLGSLCLAAGSILMSLAFSIAEFDGYTIGNFFLALGGTFVFLPSFQIANAFPKYGGSIVALVTGAFDASAAVFLFYRLAYEATDRGFKPQTFFLAYLAVPVAIAIAQLTFLPSEAYKTAPQLEMKIHKAENVQQDVHSSDDELPDDEMWRRRKARSARRRQRLSKLDDLLGDQVTRRLREEKQEHKQEASGVWGALHNKSAKDQMLTPWFILLTLLTVLQMVRMNIFIATIREQYIYMLDSVELATKINDFFDWALPLAGVISTPFLGALLDNVSTPGVLLILVIVITAIGIIGSIPALWAGYVNVLLFVFLRPLYYSAMSDYATKVFGFATFGRVYGTIICFSGLVNLSQTGIDALTKSTFKGNPIPVNVSLGVGAFVIGTALVTYVTVQVYRMRKKLDEEDAITITNTDVGSIMESLLEEDEPRSYGTVYPPRQSWEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPG5</accession>
    <name>A0A9W9CPG5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Beta-mannosidase B</fullName>
            <ecNumber evidence="3">3.2.1.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Mannanase B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_003464</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.</text>
            <dbReference type="EC" id="3.2.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism; N-glycan degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycosyl hydrolase 2 family. Beta-mannosidase B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.25" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPG5"/>
    <dbReference type="OrthoDB" id="2866996at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004567">
        <property type="term" value="F:beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006516">
        <property type="term" value="P:glycoprotein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000118">
        <property type="entry name" value="Beta-mannosidase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000050">
        <property type="entry name" value="Beta-mannosidase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001725">
        <property type="entry name" value="Exo-beta-D-glucosaminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036156">
        <property type="entry name" value="Beta-gal/glucu_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054593">
        <property type="entry name" value="Beta-mannosidase-like_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050887">
        <property type="entry name" value="Beta-mannosidase_GH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006102">
        <property type="entry name" value="Ig-like_GH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041447">
        <property type="entry name" value="Mannosidase_ig"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43730">
        <property type="entry name" value="BETA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43730:SF1">
        <property type="entry name" value="BETA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00703">
        <property type="entry name" value="Glyco_hydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22666">
        <property type="entry name" value="Glyco_hydro_2_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17786">
        <property type="entry name" value="Mannosidase_ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49303">
        <property type="entry name" value="beta-Galactosidase/glucuronidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-mannosidase-like galactose-binding" evidence="13">
        <location>
            <begin position="16"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 2 immunoglobulin-like beta-sandwich" evidence="11">
        <location>
            <begin position="199"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Mannosidase Ig/CBM-like" evidence="12">
        <location>
            <begin position="673"/>
            <end position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4373172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="833" mass="95306" checksum="20882FDC42DF3143" modified="2023-11-08" version="1">MAKVQALSVNRLTDGWKFKQADDPAQDVWLPVKKVPTNVHLDLMEHGKIPDPFLGFNELQAEWVADKSWTYKVQLPEVAKPKDGTVYVLACDGLDTFATVKLNNQTILDSDNMFVTHRLDVTNKMNPGSVNTLEIIFASARLEAIKIREQHPDHKWVGFNGDMSRLAVRKAQYHWGWDWGPILNTCGPWREVRLETYQARIADLRVDYKLDESLKAVKGTVSATIEGTSANKVSFAVYDGEKLVFKESANISDGMAKVEFHVNNPKLWYPHGYGEQPLYKISATVSADDIDLHQATRRTGFRQGELIQEPDAIGKSFYFRVNGVDVFCGGSDWIPADSFTPRVTEEKYRQWLEMMVDGYQVMIRIWGGGIWEEDVFYDLCDELGVLVWQDFMFGCGNYPAFPTILQSIKEECITNVSRLRHHPSIIIYAGNNEDYQVQESFGLTYDYEDKDPESWLKTDFPARYIYEKLLPEVVAAESPHVPYHPGSPWGDGLKTSNPTVGDMHQWNVWHGTQEKYQIFDTLGGRFNSEFGMEAFPHIDTIRYYCTDPSQLYPQSHMLDFHNKADGHERRIATYLVENFRSKTDLELSQAEALMFGYRGWRRQWGQKRHCGGALVWQLNDCWPVTSWSIVDYFLRKKPAYYAMRRVLAPVAVAVKRAHFDWSVVHARVPKTSDFEVWVASQRLDEITATVELRYISIKSGKEIKGKVVKENIKLVPNGTTDVLSGTIDNRNEEPHVLAVRIWVDGEIVSRDVDWPQPLKYLDFDDRGVEVIPKGETIVVKAEKPTKGLVFEERSGVLVHDSAIDIVPGDEQVIKVRGLGKNDEPLRWTYLGKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPJ6</accession>
    <name>A0A9W9CPJ6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycoside hydrolase family 12 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_003571</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glycosyl hydrolase 12 (cellulase H) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPJ6"/>
    <dbReference type="OrthoDB" id="95118at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013319">
        <property type="entry name" value="GH11/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002594">
        <property type="entry name" value="GH12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34002">
        <property type="entry name" value="BLR1656 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34002:SF9">
        <property type="entry name" value="XYLOGLUCAN-SPECIFIC ENDO-BETA-1,4-GLUCANASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01670">
        <property type="entry name" value="Glyco_hydro_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040901230" description="Glycoside hydrolase family 12 protein" evidence="3">
        <location>
            <begin position="18"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4373277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="245" mass="25549" checksum="6F3035BBABCB06F0" modified="2023-11-08" version="1">MKLSTLLFASTASLALAAPTATIQKRADYCGQWDSTVTGTYTVYNNLWGQADATSGSQCTGVDGLSGTTLKWHTSWTWAGGAGHVKSYANVVTKITQKALSSIKSLPSTWTWSYSGSSIVANVAYDLFTSSSATGSAEYEVMIWLAALGGAGPISSTGSPIATVTLAGTSWKLYNGKNGNMNVFSFVAASEVKSFKGDLVEFLSYLTSKQGVPTSQILQSAGAGTEPFSGTNAKLTTSAYSLTQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ17</accession>
    <name>A0A9W9CQ17_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_002436</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ17"/>
    <dbReference type="OrthoDB" id="3266505at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF6">
        <property type="entry name" value="PROLINE UTILIZATION TRANS-ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="5">
        <location>
            <begin position="61"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4375350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="472" mass="52697" checksum="4E1C251D4B27A8D9" modified="2023-11-08" version="1">MVAPEAYGIVMHPGDEDPGRIFHQQARALVPDVIELNCVRSIQALYVMGVYLLPASAFGSSYLYLGLALRKALALGLHREFEAAELDDTEKEVRRRLWWAVYSLERCTTVKLNRPRSLDASIITVRLPSAYPPLDSQQMVNNIDHQIADAKLMLILDRAEEPTRATDGLAVEQALKAWKHSLPPSLRLQNIPPNSSSYRATFHLLLNYHFTRIAMGKLNLVTIVRDHLRCHFGEKAQTSEITDQTYDLSQSCIKAAQKILQLFEDVRLTGNLTRFSFTDFQGCSISTTIVLLAGILERDASYERNVRFGLDSLRKMAVGNGTATAGVAFVEGLQAITDEAVEKLGRMAHSPSAVPPTSQATKYAEWAEWLSRQSQDPSVGEVETRQEVDAAAGQHFNVRHSNRHTILDEANASQQMLVSPSSTQLPRSQDRADPDMLYDFNPAFLTGQHSEDSMNFMSLTGFDMLGLDFQDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ25</accession>
    <name>A0A9W9CQ25_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_001094</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ25"/>
    <dbReference type="OrthoDB" id="626167at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047499">
        <property type="term" value="F:calcium-independent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185:SF1">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="6">
        <location>
            <begin position="47"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="182"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="506"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="4">
        <location>
            <begin position="51"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="4">
        <location>
            <begin position="108"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="4">
        <location>
            <begin position="306"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="525"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="537"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4375817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="549" mass="62219" checksum="0C7415D09BED71FF" modified="2023-11-08" version="1">MLQPPTEPRSRPLSVMTTGTSATAQDKTGETWKVSVDRDNCWDDLILTLDGGGIRGYSSLLILKRLMHEVAECEKRLQDEEGPVAGSQRREFNEDELLPCHYFDYMYGTSTGGLISVMLARLRMTVPQCLEIYRKVGHELFGHRRNVLPLATKYHHKPLEKAVREIVSQYCKHHESCKGDDMHPWYPEEDDTPANESALSSLETSATWSSTGESNTGRPKQLTEKICQSICLTATHSGQIDEAYLLRSYKHRYDPNICPPWVTPYNKGIDRLEIWEVTRATSAAPFYFDMLVADPGNGKGFLGFKDGGIRENNPSYAAYSEHASLKGDHDPALLLSIGTGRPDTTNDGFAAVWPGPLGKLSILQKWSEKFAVFKNVLIKYTEGEDRHKMMRTIARGEHRWYKRLNVDEGLHDLKLDNWEKGSWTNPQTNETKVVNGGTTLTKMEKATSSYLTRQETRHIGGFQEYQPPRIVLQQVAERLVRHRRLREATKAEDLKRWQTHMGQWLTGELKPEDTMIQPAPSAPRKGSRGHHSRSKSRASSPSRTTPAQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ81</accession>
    <name>A0A9W9CQ81_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001828</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ81"/>
    <dbReference type="OrthoDB" id="5403729at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061640">
        <property type="term" value="P:cytoskeleton-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009991">
        <property type="entry name" value="DCTN3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07426">
        <property type="entry name" value="Dynactin_p22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4374752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="207" mass="23697" checksum="B2544E3DD91CEDBA" modified="2023-11-08" version="1">MNEYEEAVLFTFALLESRLDRLEYVLGGPQENTEGKPKTIPDRIHRIERSLQKLAGKTALLQEANELLSKHKDVLKPDDDLDEDDSPPLDTAQKAVLVVERATGFATIASQLKALDDQQLPTTDGFTKLAMLRPRIAEAEDRQLQQALKISELRRRNGLVNQRYKHVILLGAGRCWVEYHEKLTKGLRTVVREEFKRKPEQEEAEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQI9</accession>
    <name>A0A9W9CQI9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_002147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQI9"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4375067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="178" mass="19959" checksum="71D6CF1400E36356" modified="2023-11-08" version="1">MSTGLPELGFTYLAASKICFLAGHHFEEEFNAVPDANNIAWEVQDHDTATYFEQTIPIGHVKLYKILWKVSAYMVRRTGAIHLCLNGARLIRSHTALVQLSSPFLHLQKEGEGLHLEQLEVLCQLYFLSAEYVMGLKVKNKWFESLKLAFEAVGQGMGTLEKPVGAVAWADEEGEMDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQU6</accession>
    <name>A0A9W9CQU6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002454</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQU6"/>
    <dbReference type="OrthoDB" id="5419219at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="532" mass="59938" checksum="C38DC63BE96C6AAD" modified="2023-11-08" version="1">MKRSSAKTLDSQATVPSNDTPGHEPHSTTHREPLELEGYKFNLSEEKVFEEATLHNNFVYLDKSRLSGLPRFDSLSVYDVKDEDYEKLLRLEIKNLINTAELEAPYLFNSAGRLGLCNTASYEELSLLARRISLDCSVIALDNATMVIDNRPKATLVSLAVSSMKQPCGMLRHEYVTGEKYNSSKPQSPDDSEASRKLYFAFVDFVIGSVARLLIRTASSAYWSSQTLGPLAQMAVKLKFLSKDSREYAHRAITRVEETMRSFQQSDEHRTTIVIQEHVEGKGIEEKHLQMSDDLALDEIYFIEGFIKRGCLHGLFQSAICSLQQNPIRRCISGDFHQHAVAYETGFDRSLVIVSEDREGYVTTTSAAALTRTCCRALTLVKKNIEMLPGRERPPSSSAHTGTGTEFCNITWDWAVKHADNTKPTANSTKCSRKFETKKLDSIISVMIPLALSTPYVASTIEELVQYTMAHSTRKTEEPVRRFKHSPIEASFSIQTSFNARMQKKMRDQDSDTVSINYSDLIGRLKGNSTFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQZ7</accession>
    <name>A0A9W9CQZ7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000246</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQZ7"/>
    <dbReference type="OrthoDB" id="10028886at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="546" mass="61696" checksum="29B725AD27D2AB43" modified="2023-11-08" version="1">MASPALPDDILHVLCEELANQEQFDTLFNCACASRALAVPALTHLYRSHHQAPVRGGGEDLYGLPPAIKLLTIQRWSILWRSIIASSLDATLFPYCRYIKLLDFRDLGYLLDDDQFRVKVSKQFFSGPLKQFEKLETRTTIKGKKYTRLKNADIIDAIGEVVTQHTPTLEIISGELLSGALVRWTPRLPRLQCLELWDGKPLEDELVHASLYEHCPQFSSLMIYTWMSEDNDHKFSKFLSSMRPNSLKTLQTISDIRAGAETFLALNHHGKSLEDLRLCVSNDALPHLSLLQGCTALRNLRVEDTHGSIDLETTQHDVFLEMIAWLRRCESLQRLSFSKLQSGAAIITPVLLEHNIDLISLEIDAYSLKEHKTFHQALVHQQSSLRYLSLSGDTDGMFRDDLDILVDSLKQLHELRDLKLILPEVLRDEHIIAIMKDLTRLEDLYVSGLELNDIVLDSLAGLPSLRSVTLSGISKFTVEGLLNFVDRLGPGNQGIRVTIDMADPDTLLPEENLNVIRDCLTEKTGGTLEYMELRDPNLSEFEGDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR30</accession>
    <name>A0A9W9CR30_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CENP-V/GFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_001293</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfa family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR30"/>
    <dbReference type="OrthoDB" id="2212170at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1590.10">
        <property type="entry name" value="glutathione-dependent formaldehyde- activating enzyme (gfa)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006913">
        <property type="entry name" value="CENP-V/GFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33337:SF33">
        <property type="entry name" value="CENP-V_GFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33337">
        <property type="entry name" value="GFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04828">
        <property type="entry name" value="GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51891">
        <property type="entry name" value="CENP_V_GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CENP-V/GFA" evidence="6">
        <location>
            <begin position="10"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="178"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="187"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4376013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="260" mass="28922" checksum="8C252CD6859A41C9" modified="2023-11-08" version="1">MSKPKPFPSITGSCACNTIRYRLLTSPLFCHACHCGDCQKYTGSAFGLFLNIETYNLSVISPTKPVVVIQEKKPGVKGRFLACPKCKTELWGNNVLGAAVCDLRVGTLDFPSLMEPDLHTFVESKVDWVRLPEGAKSTPKGYDYREVWPKSSLKRLEVAVARVAQATKEKQEKLATLLAKQRQENTSESDEVNVEETGGEGEKTPTAVEFEDNDVEDDEAFEKRFKETEKALQSRLEKLSLKLEEEELARKTGELAVGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR59</accession>
    <name>A0A9W9CR59_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR59"/>
    <dbReference type="OrthoDB" id="10266568at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="197" mass="21690" checksum="4E20D8AC4377744D" modified="2023-11-08" version="1">MSGLENALFNLKFTAKSLNRQALKAGKEEQAEKTKLEKAMKQGHSDIARIYAGNAIRKQNEKLNLLQLASRVDAVAGRVQTAVTMRQITGNMMKVNRSLDVAMKSMSPERIASVMVDFEKQFENVDSATELYQDITSSATAVGTPQEDVDRLMAQAADKAGVDLQEDLQAATPAKTKVGPTEQEEDAFTERLRALRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSH4</accession>
    <name>A0A9W9CSH4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">aldehyde dehydrogenase (NAD(+))</fullName>
            <ecNumber evidence="5">1.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_000944</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>an aldehyde + NAD(+) + H2O = a carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.3" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSH4"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000014">
        <property type="entry name" value="NAD/NADP-dependent betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000007">
        <property type="entry name" value="NAD/NADP-dependent betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009725">
        <property type="entry name" value="PRK13252.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="20"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4378112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="485" mass="51509" checksum="C432CEE7C08828D5" modified="2023-11-08" version="1">MSLPYAPRFAFYDGKLQSGSSTSSFQTIDPATAKPIANIHTTTHAAIDSAIVSAQKAFPAWSATPPIERARILQRAAALLRARNDELARIETSDTGKPFTETSTVDVVTGADVLEFFANLVGGGGMNGETAQLRPDAWVYTTKNSLGVCAGIGAWNYPIQIALWKSAPCLAAGNCMVYKPSEFTPLHSTVLAEVYAEAGVPPGVFNVVQGGGEVGAYLTQHPSIAKVSFTGQVATGKKVAGAAAGGMKYVTMELGGKSACIILPDADIDQAVDGAMMANFFSTGQVCTNGTRVFVPEAMKSAFEARLLEKINHIRPGDVHDPNTNFGPLVSKPHYDKVIQYINHGIKEDKATLLTGGPDRPSFLSSSPAYHSGYWVRPTVFTSCTDTMKIVREEIFGPVMTILPYTSVSECISRANATQLGLAAGVFTRDLSLAHKVVAQLEAGITWVNTWGESPAEMSVGGWKQSGVGVENGKRGLRLGCGIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XWV2</accession>
    <name>A0A9W8XWV2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010773</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XWV2"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="110" mass="11991" checksum="9A8B411584EA297C" modified="2023-11-08" version="1">MGAILTISLGKQTQKQQQQNQHADAGETLLPPSPQKKEGDDAPKQNGKRKPEGEAEGSKAKTGRPPKKKATSKKSDDEDDYSEDKENVAAPQSGQPRRSTRNTKTIYTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXX7</accession>
    <name>A0A9W8XXX7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nucleolar 27S pre-rRNA processing Urb2/Npa2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010603</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="160374at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052609">
        <property type="entry name" value="Ribosome_Biogenesis_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018849">
        <property type="entry name" value="Urb2/Npa2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15682">
        <property type="entry name" value="UNHEALTHY RIBOSOME BIOGENESIS PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15682:SF2">
        <property type="entry name" value="UNHEALTHY RIBOSOME BIOGENESIS PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10441">
        <property type="entry name" value="Urb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleolar 27S pre-rRNA processing Urb2/Npa2 C-terminal" evidence="2">
        <location>
            <begin position="1267"/>
            <end position="1500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1403"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1403"/>
            <end position="1414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4362509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1512" mass="168427" checksum="CD946185E6B2A0A5" modified="2023-11-08" version="1">MAPVKPASDNGPAPTRPRLQSINQDFSNLDEQLRQAAHIIGLPDDWITIEEGDVRSSIIRRLVRARAEWVLRWVLDKLKEETDAGKTARGNAIAWKLLDWMMHVLPVSRSAPHLRDAGFPSILERTLLENFDKATSLSSTSTSEDVDMEDASDSTETIREKAQTSRKQKRGTADTTPSKRVAHGFSDRVQLFRVVRSAVASIATLATASSKNDDTTQSELMKMVLRTESAQASRILRFWFTAVHQIITTSDTLEPDMHHYLDLSLALEIWELRVVDSKDEAGASADDFSTECLVPMLTLLDGLRAMRRADSALSSSMTIDRATQAIDKLLSRHLFAPSRVAFFADTPSESSDSGSRVREAKSLASYLEPLRAKLLQASQVEDAGEVLPADLALLFDAVPYLLDIVIRASPSRTPKGRLVEKPWIQAAFRSLAECAGCSLDAPPTDVTQPSAVTALEGALRTLQLHSVSIDTETLTNLFWYHCGVKYPEHRDKEVHWSLIAALIELDALIFVTEPKSHMKTTQKQQADLAEFIFEQISIVEFNGTGFADNALRITGDAEKDHTRPGHAADTRTGRTVILEQIVVPLMSAFARNRNLLGFLRRWDHQLVRSYRHENRKALKERTDPIWEDRTINKALVDLFEHSLTQGQIATSIQEHAKRMVELGNAMETETRENVNVKKLAAYKNASSSAVIIPAMLQSIRSDEIIESLKPHLHSLLMSYTTWVQVDRYSLHSRLAFSWFTLCQLLSKLWPIELHSSAKMQKELLHPLVEQATMDTSSSRKDQNGRRVDSLTRAAAMLFLLDVCDHLQTVPGSRELIQTSLRKIMTSLSSSRLEPKEYAKMIEFFCADFVTLFGYLDADACGESLKTLLLRLSKLDTENGSLIGCSLSQSIIEEGSSALKMAYYTALLEILSQDNEGRLHEMVIKAFLHTQPSAFPREKRETTLDRITELIGLEPQATTDLLSVMAHLMVVPNATAKLCTNAGIIFSIADKLHQQGQASPAALQLFQQLVQSILGHIIPNQEQVQNKSFLDEFASKLKPITKAAKQCSAPRLSILRATIMAQKQVELLDAEQYVKLLQQCLTDDGEDTASLEHALDAFNEIPISALEKAGRLDQTRAWLRKWIEDNADVESYKTVPSQSPVELVNYVARLHKTIARFGLYSSAKWIIELTLRLLQESAASGMKGSILDALKEALASLSTQEQLEVIPILTNVVNPQDRAASYQILNDLITTWDDKVEEDAGSKQQQLALLPRICVLLADSADHESFNALLTCINTILNDKPSLASQHSIECVLSVLVKLTSRSSPALAPTHATAIYTRLCETSRLILLVHRGRLGGRFHLLLPLLQGLLFCLFIPNASRSGALPSWLRTPTGIDPVRLTPANANQYSRLLSTLCNPPQSSITKAHHYQAQTSSRASKSKDLNDPVKAARERTSHFLYPLLASFCRFQLNGRLEAGVREKLMVGVYEVVGTAGLHKEGVDAMFAGLGRSERDVWRGVWGEWEGVRGRKEVFGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY53</accession>
    <name>A0A9W8XY53_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010607</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY53"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01365">
        <property type="entry name" value="KISc_KIF1A_KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000050">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3 4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="7">
        <location>
            <begin position="21"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="486"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="394"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="520"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="121"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4362513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="584" mass="65844" checksum="10676FE653E5C090" modified="2023-11-08" version="1">MRASEPYALMSPPPMNDDPGNVKVVVRCRAFVRREKEKGTRCLIRMDPATQKTTLFAPAEEDSSNTRRVFEDKEFTFDRSYWSHDEADPHYAHQEDVYRSFGEEFLDHNFEGYHTCIFAYGQTGSGKSYTMMGTPDNPGLIPRTCEELFDRIAHEPSPNTNYHVQVSYFEVYNEHVRDLLTPKTTPPIYLKIRESQKDGVYVQGLTEAEVRCYADVERLMKVGDMSRTTASTKMNDTSSRSHAVFTIRLKQITHSLLSDETIERTSRMRLVDLAGSERAKSTEATGQRLKEGGQINKSLTTLGRVIAALADPRRHGTKGRRPREVVPYRDSVLTWLLKDSLGGNSKTAMVACIAPADYEETLSTLRYADQAKRIRTRALVNQDCMSAAQRDAQISEMAEQIRSLQVSVNAASQRKREEATELDEYQRQVALMQRLMEENRQVSAAKIKALSSEVEELRPANTALRAEIDSLRRHLALALGELKNPIVLPPPQSVGSPDFEQGEWLGEGDGKENSLRAPVEDEVSDDDSDSGYDEGDHDELAQELQNEAENFLKDLGVFRKKVGSDVERFGVREALAQIIGNGDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYA4</accession>
    <name>A0A9W8XYA4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Guanine nucleotide-binding protein subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">GPA1</name>
        <name evidence="8" type="ORF">N0V83_010399</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the G-alpha family. G(q) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYA4"/>
    <dbReference type="SMR" id="A0A9W8XYA4"/>
    <dbReference type="OrthoDB" id="5817230at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein G(I) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000051">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000692">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002975">
        <property type="entry name" value="Fungi_Gprotein_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF302">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-5 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01241">
        <property type="entry name" value="GPROTEINAFNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="150"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="175"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="200"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="269"/>
            <end position="272"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4362306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="353" mass="40891" checksum="2E40C4CCB0EFAF76" modified="2023-11-08" version="1">MGCGMSTEEKEGKQRNEEIENQLKRDKMMQRNEIKMLLLGAGESGKSTILKQMKLIHEGGYSRDERESFKEIIFSNTVQSMRVILEAMESLELPLDDQRAEYHVQTIFMQPAQIEGDSLPPEVGNAISVLWKDAGVQQCFQRSREYQLNDSAKYYFDSIDRIAAPDYIPNDQDVLRSRVKTTGITETTFIIGDLTYRMFDVGGQRSERKKWIHCFENVTTILFLVAISEYDQLLFEDETVNRMQEALTLFDSICNSRWFVKTSIILFLNKIDRFKEKLPVSPMKNYFPDYEGGPDYAAACDYILNRFVSLNQHETKQIYTHFTCATDTMQIRFVMAAVNDIIIQENLRLCGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZL8</accession>
    <name>A0A9W8XZL8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxylase-like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009349</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZL8"/>
    <dbReference type="OrthoDB" id="447346at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053863">
        <property type="entry name" value="Glyoxy/Ble-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22677">
        <property type="entry name" value="Ble-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyoxalase/Bleomycin resistance-like N-terminal" evidence="1">
        <location>
            <begin position="11"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="106" mass="11244" checksum="2E94134C91B8E90F" modified="2023-11-08" version="1">MVLHGQVSGIEIPATDVARAAKFYSETFGWKFQAPSAEISASEMQAFTAPGGNMPDEGVVAKVEEIPKVGAAKIYINVDDMQAAIDVSLESPSGQDLVFSRVNALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZM2</accession>
    <name>A0A9W8XZM2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010443</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZM2"/>
    <dbReference type="OrthoDB" id="1935484at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000247">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000106">
        <property type="entry name" value="MFS transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF14">
        <property type="entry name" value="MFS GENERAL SUBSTRATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="425"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="456"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="491"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4362350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="550" mass="63296" checksum="BAB59FA698B47EDD" modified="2023-11-08" version="1">MAALAIDGKDVSRSSDSASSQTSQTSQTSHTEPPHHGSYENHRFSDHVTAQYWREVYEAAKYEGRHRFDPNYTWTAEEEKRLVRKLDWRITLWAWVMFISLDLNRKNINRAISDNMLKDLGMNTNDFNYGQTIFLASFLFAELPSGLISKKLGADRWIPSIICAWSIVSASQCALQTKAQYYVLRCLLGLLMGGFIPDIVLWLTYFFKSNELPLRLAWFWTALSTCNIVGSLLAAGILQMRGLNGWGGWQYLFLIEGTITFVIGVFSYGLMPPGACQTKHWFRGKNGWFNEHEEHILVNRILRDDPSKGDMNNRQAVGLRLLFKAASDWEQWPLYLIGLTAYIPPAPPSNYLSYILRKLGFSVFQANLLAIPSQFLYAIQLLIVTWLSDKFKERSIISSLSNIWIFPWLVALIALPAGASSWIRYALLTGLLSYPYCHAILVGWNAKNSNTVRTRAVSAAFYNMFVQAGNMVSSNIYRDDDQPLYRRGNKILLGVCIFNIFLFYFVKAFYIWRNKIRDRQWNAMTKEEQEDYVVSTKDEGMKRLDFRFAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y071</accession>
    <name>A0A9W8Y071_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_010515</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y071"/>
    <dbReference type="OrthoDB" id="3231000at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4362422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="346" mass="39095" checksum="363116FD28B3B2EF" modified="2023-11-08" version="1">MEVTPIRLAAAPGSSYDISVTIESENGGWTAILWSDVGKRDLSKSLGFHPFIKHLTACSKTGLRSIKLIPPTLVGIDSELMHPLPPYPSTASNFTNADDGSLVQPATYLASESNYLIDHAIAAQDPLYALSPVFRFAAATENQVLAILTKRYEVISSTIWDPEKSTEFLEQLILNKHILDDHACRHDDVLCFLRSQKLAKWAVNLTEEQTRISSDTKRAVEADYEYLLDRTRRFSVYHQEAISVLVSSVALVESRKQITLATQVTKLTILATVFLPLSYCTSIFGMNFIELEELRIWIWVVVTICVGIVTVVVYQWDQRSYWFHFLERSCRVRTFRDPMLSGSQIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y088</accession>
    <name>A0A9W8Y088_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_010182</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y088"/>
    <dbReference type="OrthoDB" id="3800703at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019783">
        <property type="term" value="F:ubiquitin-like protein peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="4">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="252"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="514"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="679"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="263"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="286"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="528"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="545"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="565"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="766"/>
            <end position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4363062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="963" mass="109081" checksum="9C381FB1AE94B378" modified="2023-11-08" version="1">MTITWEASQLYRAAMHSGYLVPADTQDYKTNWAPMVNDFKSHNIVVIPVNNGFKAETAPNPSTTQQPGLARGSHWSCLVIDKHDANLSIARYIDGTARAVKGDTKWMVADIQMNAPIAGHVLCGFETLLGVKRGAMKTSSLKFTPHMELADESTLDDYGRCGPYVYAFLDYLLANKRNLIDSPGLKARFNPNERRKREREFGFNSEFTRKKLQYELFAERANQEPRYNITDEVFHDLRSRLTFEQFISLARDDTNDLSPPQKNDYRPSPDNDRARNRNRAGDGMQKSLQKELSTTRQNTPEASLGVLKVLTKNPNYRFPEGYSKDQLPEDFAQLRPDEVKQWHDLYRKDLFNLGDSGRSLDNPTIKAVLHRVFKGSFENEPVDSLRTYADSSTFFSPSERAEQRSKPAGELAAWLDTHLLELQIPQFASLSNPQIDNWIVNLHPDAKDVVQDRDGNNLHNRARGMLYRFFVGKIEDMTDKEVDEWRNNDPAMSADDKNDTSSARRWLNYNHETTKPVPYLKDSPLHWPRKEHPLEQGHKRKRSDDDNDGNGDNDNDAHQRKKGPKHDDKHGKDDDNMNSRSIDYWLRVDAETLNKYVTDDIRNDPRIGEHSNEYTYRAILYVQHGGLFTGITRLAHASSWIHDVNVFTTSKDRNASGVDAVIDIKGEVDFRTSRAEVQRRMDAKYRPTDIQPEQAPVGGNKNGRGPPKKRVERPKPGGAPPKSDVGPPKPNGRPSKPGGGPNKSAQAPPKSALALPKSTPPPSRITRASSKVSQASITSAQQPSRSTRAASKANQSPPKSNGTTVKFKPVYRKFSEAATSNLPRLNRAALSQENVNFTRMTEEQVNDWVQLGWMQTPVNHNLDLWHNKTLLQMCFGGLATLSEDDRTYWMANDPLFASNNDNQNPSKFVATLKERVADSVIMDNRGHDHLYPDWYLAHFREGRKIRVMGRRGRKGWGGDGGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0D4</accession>
    <name>A0A9W8Y0D4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010187</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0D4"/>
    <dbReference type="OrthoDB" id="5413827at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="41932" checksum="0CCC59A52EB0F799" modified="2023-11-08" version="1">MTTGITPLPGSDPDLDFTSSNDDDEVEFLREMCMGKEVVPISDDGEDDSQPNSINPAWISGKAFRLLDLPAELRVHIYQFLLPHNLTISFRSLLLRGNALRWIIFVQPKSEDVSTYPPSFLYQVDGPVFCTHPSVHTQLLLVNKFVSNEASAVLYGSNTYKFGVDGLPHYPISLQSPLIFGPLGTDVHLPLLRNLRSIHINVRLDQHGRWTVIRQRARLDYFVKILKEYADDENKKSLLQELKIELKPPTSNGRVGVLPPTTEIEQYMFSLESLASLRGIKHVEVIGPLDWFAQCLRLCIQGKGGDVQEVEWPLVKIQRRRGLTRQKKDAWVTTRKWYQPMLNWKEFAERNGIPLPEDVDKFWAAND</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0I4</accession>
    <name>A0A9W8Y0I4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0I4"/>
    <dbReference type="OrthoDB" id="3794856at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="408" mass="45053" checksum="ACC81DEFCDFFA0F3" modified="2023-11-08" version="1">MSAPFFTNVEMKDEGTPELAPGAQEQMSSSDRTEPTTDGTGPTTPSHYTSPFGDVAAFAADLFHEEPGDTTFQLIDAAVSDFDRKDSAYHDAGQQLYHLINERLGKVSETTPFVNHHSAQNSYNQGPPVEQPPVEINDTVFGYEGYHPALTAPGEGISGQLLPPPPILPIPSIIITPPSPPQPLYQPSPRPDQPRRQHPSSPPTKRPPLHLPPHSPLLSPPPTWRCPQNDMTIPTTDPQRQFYASHLLSAINNLTNIIDKPTPAFHKHWLASPPFYTPTHREHLAWTILPLTTTLHTSGPASIFTLPNSFDRNFSEDVYKTRAWTFRERVGKICALLAASKARCQRALGGSGVLRIVGCPEKLLKMARGDSRMNERRQEFLEVGREVLMGEIEMEMDGEGEEEGGMIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0U5</accession>
    <name>A0A9W8Y0U5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA pseudouridine(55) synthase</fullName>
            <ecNumber evidence="3">5.4.99.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PUS4_1</name>
        <name evidence="8" type="ORF">N0V83_010137</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pseudouridine synthase TruB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0U5"/>
    <dbReference type="OrthoDB" id="9995526at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160148">
        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01080">
        <property type="entry name" value="TruB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014780">
        <property type="entry name" value="tRNA_psdUridine_synth_TruB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00431">
        <property type="entry name" value="TruB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767:SF2">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE TRUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767">
        <property type="entry name" value="TRNA-PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase II N-terminal" evidence="7">
        <location>
            <begin position="57"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="326"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4363019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="377" mass="41810" checksum="A01F7C90B43542EE" modified="2023-11-08" version="1">MEQTAERVLEGVFAVAKPAHLSSADVLQKLQASFAPSQIFAPLLKHQPKRPSKDDHQVFKMGHGGTLDPLAAGILIVGIGRGTKQLQQYLACSKTYETVVVFGASTDTYDCTGSITEQADYGHVSKDLVEEKLADFRGTIQQQPPVYSALKINGIKACEYVRRGKELPRELEKREMQVSECTLLAWYEAGKQDIAWPGDKSPIPAPAARIRLTVCSGFYVRSFAYDLGIACGSRSYMSELLRTRQATFTIREPPESPHLISALTYAEFEAGEDVWGAKLKPQLVGWIETNPLTDGHVDGRHRDTKDKLAKEKAEKPIQRFRGEWMAGDKKERIKQQGGKFKGKYNRRPAGVLPQGHTREKPGSADLIDTDVLLPTNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1N0</accession>
    <name>A0A9W8Y1N0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_009559</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1N0"/>
    <dbReference type="OrthoDB" id="4456959at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14724">
        <property type="entry name" value="ZIP_Gal4-like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF4">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="54"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="20"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="122"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="679"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="127"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="142"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="679"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="740"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4364104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="882" mass="97344" checksum="3D3808AE1FCE27E3" modified="2023-11-08" version="1">MAKRPSDADEPNLVSKIQRIEHPNPASPHQRLTNNDFSGSVKRKLADAKRTGQACDRCKIRKIRCDGRPEGCTPCEQNRTPCRTTDRITGRATVRGHAEAMESENQYLRNQIADLQAQLKEAGVEPRPTPAYAPPPPPSLPQDKSWSETPQRTSISPLPGYAPAAGVSKGELQSLPQFKHGSIGDNYLGVAASDSLLSHIKGTSLSVFGTEIDITDFMTSEAEYENGPMSYTTMVKISVGAHTVEAPPLPPYETLHDYATWYLRSLNPYTMLVHKPVMMDLIWRFGGNGGSFTPTPPQIVTVHMMLATIQYQIATRNSQQGALMEESHAHYQYALSFYKHLLLGRHKLEDVQALAMICHHLRNFPKPGAAWILTSFTYLFAIELGLHRSVTAWADGTKKLSKLDIEMRKRVFWTLNALQVNLNGKLGRPMPISNEDIDVEFPEPMNDCLPGEEANLDSFHQCSFQVGIQIAKYTALEVDLYRTIYAVRHSPRAYAENLKRLEAGIQQWKDELPHELQDPSRASQDDHIFALYLEYWYQAYLLLLHHPAVCRSVDPAIISSNLDKCLDASQKMLHNCTEMMHKKSLDIPWINTVVYIAATFTTLFVSSMRKERLTPVDMTKLKGDMATWINVLGECDHFLGSGDKLKNAIAKIVDQSLSNINDSIVKRTATESLARVALQTPQHTSSAPVYDNNGYQEQYGATSSPTDPALTPQTSGYGSIPGGANTSHPYNIGNPLAVAQQPSSGFDQQTYSSGEDTGMNPTHAAALAAATSGNTPQPTSNAYAYANSQMVNSGHQPAYTANGFAPQDWRQWTRTYMQPQLGQPGEYLNTATTLMTLGRGESQGPGNDGQGLVDNAGVQEHVGPHWPEISFSGTASGHLGHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1V9</accession>
    <name>A0A9W8Y1V9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1V9"/>
    <dbReference type="OrthoDB" id="419598at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052718">
        <property type="entry name" value="NmrA-type_oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47129:SF1">
        <property type="entry name" value="NMRA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47129">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="1">
        <location>
            <begin position="2"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="306" mass="33827" checksum="984243126DFA3914" modified="2023-11-08" version="1">MRVVVFGASGVQGRHQVRVLARAGHSVVAISRNPQPLVVDDKYPVTTTAADFTDSAAIDSVLHGADRIFVNLPSTSFNQAGPILAAAKAIGSAAKKANVPLILFNTSMPVPKEKQDIKAQDDRREMKKLLRESGVPVISIEPVVYLDNLLEGWALPPIRDRNTVVYCHKPSLRVSWICHHDVAQLMAAAMHRPDLAGRDFPVGGPETVRLSELAAKLSRAWGRELSYENQTVADFCEKIGKSMQGRGLDTETIVSQMFRAYTYYNEAEDEPFNVDMGPVVRDLGVRLTGIEEWAARRSPWDDKGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2B9</accession>
    <name>A0A9W8Y2B9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_009237</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2B9"/>
    <dbReference type="OrthoDB" id="271862at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22453">
        <property type="entry name" value="KH-I_MUG60_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056553">
        <property type="entry name" value="KH_Mug60-KHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10627:SF76">
        <property type="entry name" value="KH DOMAIN-CONTAINING PROTEIN YLL032C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10627">
        <property type="entry name" value="SCP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24563">
        <property type="entry name" value="KH_Mug60-KHD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="273"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="568"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="655"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="855"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="942"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="871"/>
            <end position="885"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4364641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="985" mass="109555" checksum="6DF0F4ACECE3C80E" modified="2023-11-08" version="1">MHKISNFTGQARHGWEKMAPSTGFPFSRSNHEMPVTAPIKRPSTATSAPPVIPPGTQVNLSFNVPFSSNLAGPDPDDIMWASPGAKARWTHPEGTAEGTPTHKLPVHAQNVDNLRNLCRDVSEQTEGRVSATVTSAESKPLPGLQRGPLRALVTNVCLSGELEIVNSMRCKLLNSTPISLRSSFVDIDSQLIIDPPSQNVKVPVLQQLDLISKTTKADIFLLMPKQTDIETASFHGNIDTGMDPRLRCAIYGDLETMEHAKTRVLIMIDQILKRYVDTMKLELTMHTLICGRGRKNIKMIESATGTAIYFPPPFPRLFGYTPPGAQRRPDDLVYITGENSECILRAKQRLHDLVMSTKVFVKPVMVTDTKLDAILLERLDKVRKIIENNGSYILLPPLGVQRGVVRVQGTDVLHVERTVRELMQLAGQFYSASWFITHPDPSQRPPTSADVRAMLSDICINSGAEVSFEKLNFHIYGSDDAVRAALMVISNIPFVKKSQYNMSVKIELANEHKEFVSGKKNGKINKIMSHSSVQIVFDGFNEYNFYIVVRGAQYEATKNGLDLVEQEMPASISFHVPDQYHKRIIGIGGQHIQRIMKKYSVFVKFSNAMDRGGFGKEEDDVKVDNVICRTPARNAQSLDLVKQEIMDMVEKADAEFVSETININRLYHRELIARLDKIDELEKKFNCKITFPSTEEASDTVTVSGPEYQVPLAVDEFLVGQLWVSLRMITNSKKGMVPESHDLAFPATTDLSEHIRSPEFKADILDKLKTQHVVDAQLQDTQTETIEGKEEQIESFHLVFTRNNAGGLKDAIDFLLNHLVMHGLDTSTVRGALPRPKSDSFEDSLPFFESKLLQRAEPAVDSTDSPTRSHFGGDDEPRGMLDRLRKPGSMSSFTSIFNGRKNTNSPGSLFKHASSNASKASLASLESTGSYRNPWNDSGINLADDEHHTNGWPMPTPRFESKFPFGSSSAASGDVTPQVRSAGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2X8</accession>
    <name>A0A9W8Y2X8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009563</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2X8"/>
    <dbReference type="OrthoDB" id="2157530at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF64">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4364108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="504" mass="56308" checksum="39DBFC8D4A65B57D" modified="2023-11-08" version="1">METPIHSVILANIRKGCRWGQRILAGLLTNAKLGSKVAKHSRDHFSHSVDDLSEDSILTPVLCAEYWDRVWTLQEFALPKRSLVLGKYITISADHFWYSQWLLQRLEGKGSGSSGYCFHVSLRTWLHDTMDSRNAKGGREPYQLLGSGSEEGVLEKLLGDMRALKATDAKDKVFALQALLQAAGFDIGPPDYSKSVAVVYAETTRKIIKATSSLNVLTLATTIPASAEMPSWVPDWSGESYPSFAKCDTELPRATEASTYTDDSEAGTMELNVQGIVMGIVEEVSRHCMTWRVDPSTLTDPVSSSNPDLWKDVLAYFVRLSQSWVDMAGLTKPYSEWTDEFWKRQECLASVLLAMVLNTQVAESPAAVHDEICRIIQLICEGDPSSAYYTEDFRTLIQSEFMDSPAFRAYFTIAHNKPLCKRLNSLYLANYKRSIFRTKDFLGCGLHSLQKGDLLVLLKGGNMPFIVSSTVEGWHLRGPAFVPGLMQGEAWPESEDRLTTFKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y315</accession>
    <name>A0A9W8Y315_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008391</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y315"/>
    <dbReference type="OrthoDB" id="3702761at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4365770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="508" mass="57310" checksum="A87F475700F23556" modified="2023-11-08" version="1">MALSNPPGAKPLPTLPTLPSEIRRVIVSSLAPDPDNLRPGCKDDLKNENSAHSCLREWVTEYLFRDMALPQVLPGTASHLERFSIIPANTELVKHVKHIAVQVPPAVRWEVGVDNPFSLHQEEHTFQRLCSKFGIKNPRHMSTAQHEYCSNYHRAMVEPFTDNRRWHQLYLAADRSWQRIFRSFPYLEAISVGCCGRTEQPAYTYTHVFVLQHGKSVVEDVYPKFVEDATVNMAWASSLVCAYAPPSVRSLSLTMANMDNLNSSATINRLLSLSYSSRADMNLGLWHVTKVCLTIRGIAGVHGAKDWDGDTGTAGSVRYWKKVLNLMQGLQHLELRNGLTADEEYRMTALEHTDTEESVLGWLLPGLVANQLQTLRLADFSFNRATVTTTLNSGHWPYLRVLVLDDVQLKLYEKPEFDDVMNILKGRAWMEVCLELTEQHSGLCIELNRPVSSVVGVIGGAADHRLYSKYVDEIEGIPGVQVDVEGYGQYVKLTEEEVEVEEEVHKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y322</accession>
    <name>A0A9W8Y322_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008406</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y322"/>
    <dbReference type="OrthoDB" id="424974at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF40">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="363"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="410"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="383"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="410"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="470"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="573" mass="63920" checksum="6C048917CB0435B5" modified="2023-11-08" version="1">MGILERSLEEGIARKSQVSTGASSAPLQLPGQEESYSDQEEEEDTKDLDPSHLATEDAAYYEGEDNDDVVDLGIAMGKVRITERIGGLVRPRFSEELAQALKELPKNLRTAEATGPRPQIRPDDYDTRFPLNIDDSDLDRAENGEGGVDVTKDRNYFTDMTITRMRFECYEMHRFLWNERPKLESRRGDGERKVTITSLLSRIQSFKAAMEKTYVPMLNRTAPLHALASEIYGIISDRLYIMLLQKYLSSDRSKMPERLRQVIMSAAAMILEHSMTIEQQPALSTWSWYVGALHQYHTALLLLNELYAGSREPAFEQRVWRCLDYAFELPSGPTDIEKVRMVLQDLVGKTQIYAKVKRVRIPNQMPHVGPRTHTPGYQARQQEVGEDRERRESLQSASSIAGFASFGIGANSVGQQSSPRQQQPQSCQRQQSQSQAIPFPGSIPNVDWGTIDLPASAANFQLPQQPLSTLDSFNRSESIPTTSPGGLMPSNMMQSMGNISESNASSPGAAIYGSIPVGTSNSSPMDALNEIDWNDIEQLFGNAEMDAGNMLIPPFTFPPFSAIDLQWPAQDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3C5</accession>
    <name>A0A9W8Y3C5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3C5"/>
    <dbReference type="OrthoDB" id="3795696at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02178">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="410"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="464"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="563"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="606"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="644" mass="70210" checksum="41F44A19E6E1D838" modified="2023-11-08" version="1">MAPNGHAAAGRNEAHQSRSSSLRNWLDQYGDAEFRTWQDEHPTHDLTYDDWKHSEHVDGLSLYYATLSLFEEPDSSDEEAEKVEVKPRARAVRGQTARESKKKVTMNGNGPSRATGSATPSTLPKPEDLSSPGKRKRRARKKYLSEEIVASDGDSELEELGASTPIAEAAKPVAPVNTVDGRRKSSSVRKASRKKILSDEIISPEDEIDDPVTIDEVATPAIVSPLPVRSAPKTSTPAQSVEAPKKTILKLSTRRAPKKILSDALVVDEDASDARTPAPALTVKKTAKPSANASTGDTVDGDSGIESKAVATKASSKVTDASAASTRRGLRTRRPAQKRPYFHDALDAQLFDDIEPEGSDATDLSPNARSRRASVASLSKDQDESLLEELHDESIAVLQDDPELGSSERRPKHFKGKGRAWKKEESDEDEEFTIAKRKAAKAAKAKAKGQAPTQKKRGRPRKSVLSEDLVRDESDSDAVEKEASVKESKSPVRAGREPLKKAKATPRKSVLSEEIIREDSESAAEDKPNIESTDSVPALEVPTVEPKKRGRPRKSDQSTTSKSSDHRHEEEEVHPHGESHTPQETPKSKRTPENPEAPLSSVQGTETKESNSHMEVTEPAKDNELAAARTSGEQSVNGDEEPRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3G9</accession>
    <name>A0A9W8Y3G9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl phosphatidyl inositol protein transamidase complex subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">GAA1</name>
        <name evidence="3" type="ORF">N0V83_008413</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3G9"/>
    <dbReference type="OrthoDB" id="445301at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000121">
        <property type="entry name" value="GPI transamidase component (GAA1), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007246">
        <property type="entry name" value="Gaa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13304">
        <property type="entry name" value="GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13304:SF0">
        <property type="entry name" value="GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04114">
        <property type="entry name" value="Gaa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036762">
        <property type="entry name" value="GAA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="488"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="533"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="569"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="619"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="642" mass="70764" checksum="AB81DE583E4B9100" modified="2023-11-08" version="1">MALISSLLALRQDARILKIPPYLSAFCIIVGIAWLLVLPLNEYSRNTYVSENALLPGQVHTYFTGSEHNVFRAYRHEVHGLIDKPVEERSQKLEAIFREQNLKVATQHYNYTVSNRTIANENVYAVLQGPRADATEAIVLIGAWKNMADEVNNSGVALVLTLARYFKRWSLWSKDIIFLVSGDSTTGPQAWVDAYHDGHDDRYVESLKIKSGALQGAVAVDYPAGPWGHRYDKLHVVYDGVNGQLPNLDLFNTAVQIASGQMGIGCVIQRMWNHNDSYKERLQTMLRGMLSQGLGHATGPHSSFIPYHVDAVTLVTVGDGWHDEMSLGKTVESLFRSLNNLLEHLHQSFFFYLLLQANRFVSIGTYLPSAMLIAISFSISSIALWVRSGRPDDSAAANPASTEPAIKPSDKPTSADDKKVTPEKESLEDTKDTKPPSTTVVSSGNSTAIVPTTLTTITERHLLLPLLFVVAAHFIGIIPLYLFNHWDASFLVSLLLTAANASTLLPIGVGFLFNYTRPLSMREANLIQCFSLLLLGMFLAALATLNFSLSFLIGVVATPLTFVRRTNSVGWNVIQLAGLAVLNPIMVIQLGTKMLGGSLAEVLVKAAQGWHIWGLWTQVVVWLVWFPAWFAGSSVIASGVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3T2</accession>
    <name>A0A9W8Y3T2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">poly(A)-specific ribonuclease</fullName>
            <ecNumber evidence="5">3.1.13.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">POP2</name>
        <name evidence="16" type="ORF">N0V83_007401</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CAF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3T2"/>
    <dbReference type="OrthoDB" id="1164111at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000056">
        <property type="entry name" value="CCR4-NOT core complex subunit Caf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039637">
        <property type="entry name" value="CNOT7/CNOT8/Pop2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006941">
        <property type="entry name" value="RNase_CAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10797">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04857">
        <property type="entry name" value="CAF1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="458"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="483"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4366873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="500" mass="53778" checksum="B49C2A8FD5AF6980" modified="2023-11-08" version="1">MPPHGGRFPGHNLSNPFAHLSSPMQLHQQHQQQQNMQHQGFGGTQGHNLNLFGQHQSGFQSNAGIGGGLGSAGAGAGLGAAAALGGAGGGTGLDGHEARMRFAHGAQLQENAVRGQEGAKGLAGQRIRDVWRSNLHQELDLLRSLIDQYPYISMDTEFPGVVARPIGDFNSKASYHYQTVRCNVDLLKIIQLGVTLFSIQGEIPPSHLDTAQLDYKPKTLQRYANNIVVCPCTWSFNFQFSLEDDMYNEESIQMLKKSGADFDKHNSQGIDPQEFGSLLITSGMTLTDDVNWISFHSGYDFAYLVKMLSSKPLPEDEVEYRKLVEVFFPRLLDVKYLWRHANNLVRRGVIGSTATNILNNLGTKSGLQDLADELGCQRVGNSHTAGSDAWLTGTVFWEMKKKIFEGNIPEEMNGQMWGLTGVGPPASATAQAAVLAAQGAQNLSGFQGMNGGMMFHPGQARHGDGPSTPTNHPAGLASTPGPSGQGGMMTPGGYGGYGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y479</accession>
    <name>A0A9W8Y479_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007776</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y479"/>
    <dbReference type="OrthoDB" id="3433125at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053221">
        <property type="entry name" value="Burnettramic_acid_biosynth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38887">
        <property type="entry name" value="CHROMOSOME 21, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38887:SF1">
        <property type="entry name" value="RAS MODIFICATION PROTEIN ERF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="517"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="529"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="540" mass="59661" checksum="91947ECA8CB1EC9C" modified="2023-11-08" version="1">MVIAPILRTINRGISTGIGLAGEKYHDHKERSNQKKASAAAEQSKGLSTTEVTELVNPAESGDNVGDDARIWALDEAAGPPSYEESQDQQRPGVDRTVSDLVHEVAAVREAEGPIVEATAVKLPYPVIIPQRRPGSKGRGWARAYPPDLDAKGIDQDTFMQFLQNFEDASQASPWLKTLYVAGNAVGFIPGHITMAVSLSVSITAGTAIELQSRYKANAFLDQMNKDVFMPLGLYCMILLCKDTPSKNGEVEIGMESFNMDTAKQISRWGLPKEREDEESEQSTKIKLLRPIRLTSGRTTTDTMPLDIAPLVYPGLEDMVARPEIKRDENFKDRLLRNKEFVADYFDRRARAEYAGNNPDSALTKAGAETPEFRNRFADPNHPCNNGHLFSLVTGGKYVAQPRGRRSRLREVGEDGKLKPKIKQEHKIRGPISLITHPIRKVMTPNILYLTIVNLPSEQEMAEARQEMGLDQKGLKEMLAQYAEARTAGAGRGEYPHYAEARTASGSREEIVHELSAQPTEATTASGSRGEHPVYELSGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y481</accession>
    <name>A0A9W8Y481_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008229</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y481"/>
    <dbReference type="OrthoDB" id="1898734at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002410">
        <property type="entry name" value="Peptidase_S33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051601">
        <property type="entry name" value="Serine_prot/Carboxylest_S33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248">
        <property type="entry name" value="2-SUCCINYL-6-HYDROXY-2,4-CYCLOHEXADIENE-1-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248:SF2">
        <property type="entry name" value="PROLYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00793">
        <property type="entry name" value="PROAMNOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="70"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="466" mass="53068" checksum="9287A2B2B888464F" modified="2023-11-08" version="1">MIQAAKILEQRSHLVPGKLKITEHFFEVPRDYSNPASGTIQLFARSAIKAEKPADYDSKSETDPKKKQLPWLLYLQGGPGFECRSPQNVSWTQTMLDKGYQVLMLDQRGTGLSTAISQSSLQLRGDEKVQAEYMKSFRADSIVRDCEAIRKALTASYPADKQKFSIMGQSFGGFCCTTYLSFYPEGVKEAFVFGGLPPLTASVDEVYTRLYAGVKQRNEQYYTKYPEDVTRVKRIVKLLRRFGDTTVRVQGGEGYFSARRFMQLGIYFGKHGGFDDVHNLVLRADTDLTQFGHLTRPTVLALEGAQSWDTNVIYALLHEPLYCLGDGGKSGWSAERMLQEKFPEFGLDKVDDEEEPVLFTGEMIYPFMFECYPELTKLKTVGHLLAEANDWPKLYDEEQLRKNEVPMYAAVYMDDMYVDFNLSMETARTIKGCKPFVTNMMYHNGISAKTDEVLKEMFVLRDDVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4B8</accession>
    <name>A0A9W8Y4B8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RING-14 protein-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_008289</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4B8"/>
    <dbReference type="OrthoDB" id="5588846at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327">
        <property type="entry name" value="RING FINGER PROTEIN 127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327:SF51">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR OF YEAST FORM ADHERENCE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SPX" evidence="7">
        <location>
            <begin position="1"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="361"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="63"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4365669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="52393" checksum="4CA5726CF27E2F29" modified="2023-11-08" version="1">MKFGRVFKQSLTNEGFPPEWVDSAISYSQLKKCINRLTNELQQVGLDPPTLSKLLKHVEDYNAAADQDDDQERPFEYILNGDTPADSSSKSRRPFHPKLLFYVNETTGEVHSAKLNEETKRKLQMLAVETGISDLRVTEESDSTKSTEPIHGPTSRPGYRTVEVPITSDTEFFTRLTTEVSGLEKLQAREEKRMGSKIEDLGKQIATLTDPSRRSNKKMLTAWRQIFQMYIEEGIFFGTTEIDHKAHDSDKAMQRLAEFSNKVAQTGLVDQMRKKDNLKALNMFMHINREILQGLRFGEINHSAMLKILKKFDKRTALGVKSAYPRQVEYPEFSEHLAKSVCAEVNTQILSHIPQLDDYSCPMCFDLKWRPVKLSCGHTFCIRCLIVMQNKKQHNCPFCREPTVFKANSDNLDEELAAFLKKWFPDEVKTKQRYNELMAGVDQYGEVYASQKCVMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4L5</accession>
    <name>A0A9W8Y4L5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ricin B lectin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4L5"/>
    <dbReference type="OrthoDB" id="4426724at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040898123" description="Ricin B lectin" evidence="1">
        <location>
            <begin position="19"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="19376" checksum="294DD90EF6577C7D" modified="2023-11-08" version="1">MHFYSLFVVAAYICTASSTLTWSLQKASNPTADQQDAYTKIEAAMTKAVARYHKYSDANKTIRVYYAPGVPTAEASYNGDLRFGSNRSYMTERTAMHEISHTLGVGQTAAFDSKCASGDWKTALPLLRSWDGASAKINCGGGHFWPYGLNYETEWSETNANRHVQMVEAMLTDGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4X7</accession>
    <name>A0A9W8Y4X7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Serine/threonine protein kinase</fullName>
            <ecNumber evidence="9">2.7.11.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">IME2</name>
        <name evidence="9" type="ORF">N0V83_006877</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4X7"/>
    <dbReference type="OrthoDB" id="2158884at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07830">
        <property type="entry name" value="STKc_MAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000233">
        <property type="entry name" value="Meiosis induction protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000314">
        <property type="entry name" value="Serine threonine-protein kinase mak"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="19"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="356"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="602"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="366"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="378"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="424"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="607"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="695"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4367296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="778" mass="85332" checksum="4D584B9235B73F45" modified="2023-11-08" version="1">MHPESQSQPHGWASLEERYEVMKDIGDGSFGSVALARVRTAGSHIARRGTLVAIKTMKKTFDSFSSCLELREVIFLRSLPPHPHLVPALDIFLDPYSRRLHIAMEYMDGNLYQLMKARDHKPMDAHSVKSILFQILCGLEHIHDREFFHRDIKPENILVSTSQHNDASHPFRRYSALMTPPSTPPVYTIKIADFGLARETHSKLPYTTYVSTRWYRAPEVLLRAGHYSAPVDIWAVGAMAVEIATLKPLFPGGNEVDQVWRVCEIMGSPGGWVNKHGQRVGGGEWKDGVRLAQKLGFSFPKMAPHSLDTILPAPQWPSSLANFVTWCLLWDPRARPTSAQALAHEYFQDAVDPLRPKSSSRLLGRKQSEISAKDSPEASPSLTTKTSSWLRRSLVARESAPAVPQHTTTQPLSPRPSPAHANSGDVSGSTKQRPTATKRATWTNGVPSNGAPIPILPSIRPISPLSAEVTAQASVRPADGEEQAAKKIGRQLSVASHGNHYADIHRQEAERALNGQSGLVSPASGQKEGFFSHLRKRARRLSGRYQTPMSPNSEDIEANAGCAPWGATSARQSMIIDQPGLAGASSTTSDFSELDKALQHVRDSVEAHSQQTNSTKSSRVATNPMLKRHHSVPHGPEPRASENGVNGPISSRTRRALQGKSNPANRYETPNEEEELLSEVLASTHTAAKKLDNPKTVQRSQTDLGPSASYLTPSPSANRNSVGFGQSAYTTPSKPMDISKGHNKPQETVSKWPTPPYDEGDWASSVAASLMATQKLYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y502</accession>
    <name>A0A9W8Y502_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 14">Phosphatidylethanolamine N-methyltransferase</fullName>
            <shortName evidence="13">PE methyltransferase</shortName>
            <shortName evidence="13 14">PEAMT</shortName>
            <shortName evidence="13">PEMT</shortName>
            <ecNumber evidence="13 14">2.1.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">CHO2</name>
        <name evidence="16" type="ORF">N0V83_006416</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 14">Catalyzes the first step of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylethanolamine (PE) to phosphatidylmonomethylethanolamine (PMME).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + S-adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11164"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64573"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="EC" id="2.1.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="13 14">Phospholipid metabolism; phosphatidylcholine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13 14">Endoplasmic reticulum membrane</location>
            <topology evidence="13 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 14">Belongs to the class VI-like SAM-binding methyltransferase superfamily. CHO2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13 14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.17" evidence="13 14"/>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y502"/>
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    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004608">
        <property type="term" value="F:phosphatidylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Phopholipid_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016219">
        <property type="entry name" value="Phosphatid-EA_MeTrfase_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32138">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32138:SF0">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PEMT"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PEAMT"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51598">
        <property type="entry name" value="SAM_CHO2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13 14" id="KW-0256">Endoplasmic reticulum</keyword>
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    <keyword evidence="9 13 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 13 14" id="KW-0472">Membrane</keyword>
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    <keyword evidence="11 13 14" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="12 13 14" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 13 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13 14" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13 14">
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            <begin position="111"/>
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            <begin position="226"/>
            <end position="245"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13 14">
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            <begin position="294"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13 14">
        <location>
            <begin position="393"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13 14">
        <location>
            <begin position="416"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13 14">
        <location>
            <begin position="469"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13 14">
        <location>
            <begin position="496"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13 14">
        <location>
            <begin position="560"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="333"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="27"/>
            <end position="55"/>
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        <location>
            <begin position="354"/>
            <end position="366"/>
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            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4368061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="973" mass="111261" checksum="87A3D05F2AC375EA" modified="2023-11-08" version="1">MTDTGNGSNGDSSQLRERPSAKPLHLNKPDDAKQKVLELNDQEERERKDDKDKRTYGRTPDGTVFIVPHTHDMVSQLLSPSQPKNLSDLAVLAVLAGLIFTLYVLPKSARIPVFAVIFLSWRAAYNAGIGWLLDGQSKHNRLVLWAKNSCIFEKPETGKNPYPTVYKLLKREMETKIPIDYKFEEAPLEYNTWLVFRRVVDLILMCDFVSYCLFAIACFNRPPESWLLWALRWTTGIILFLFNLWVKLDAHRVVKDYAWYWGDFFYLIDQRLTFDGVFEMAPHPMYSVGYAGYYGIALMMASYKVLFISIVAHAAQFAFLILVEEPHIQRIYNPPPPRRTRHNSENLSQEDRPSTSQSDSPFADNRSSYDSIVQPAPMHHIVGPQNTDLHRSIDVTVVLLSVYTFLLATLTPNTWAIRAFLFINAFVWRLWYSLGLGYILDRQSKKRNWTRHFIKHGDSKEEAWRQWKYLYHLSMTMCHTSFLAAAWKMYTLPPDWFYGLTLLRHVLGVGMIALQLWIAASVYDSLGEFGWFCGDFFFDPPSRNLTYSGIYRFLNNPERVLGLAGLWGMALITWNAPIFYLVATAHILNLLFLQFVEKPHMQALYGQNLREMSGVSKTLRQALPSPVRNWQSAADDYLNSAVEFIEELLDNARPKLAAGVDTFVKDTTALFKTHPARISITRLPQDLAGLDTKQYKLSIEGTVSPPIVEYQKSGGREGELARTPATRTSEFKTLILEYGAPIKVRWQAPLNHSKKDWIGLYMIADNQSRKATNISSSGRWVATNPGVYDSTRAEEGILVSDKLMPADRNNDENSDCYTGEVEFRGDKLWWTTGVFEFRYHHGGRHYVMALSQAFEIRIPRFDEDDVEIDANGTIHRAVEQTLLPIIQNCFDRDPEIAPSSPEESFGSLVERDGKFAKRVVFAVHQMFGIEFAPEVVQADGNVRNLAWRICNAKKVLAPYSMSASRGRNTPTMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y589</accession>
    <name>A0A9W8Y589_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Folliculin-interacting protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006537</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="5428015at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042030">
        <property type="term" value="F:ATPase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028084">
        <property type="entry name" value="FNIP_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21634">
        <property type="entry name" value="RE13835P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21634:SF9">
        <property type="entry name" value="RE13835P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14636">
        <property type="entry name" value="FNIP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Folliculin-interacting protein N-terminal" evidence="2">
        <location>
            <begin position="78"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="334"/>
        </location>
    </feature>
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            <end position="390"/>
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    </feature>
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            <end position="489"/>
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        </location>
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            <end position="646"/>
        </location>
    </feature>
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        <location>
            <begin position="655"/>
            <end position="667"/>
        </location>
    </feature>
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        <location>
            <begin position="669"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="821"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1129"/>
            <end position="1139"/>
        </location>
    </feature>
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        <location>
            <begin position="1186"/>
            <end position="1201"/>
        </location>
    </feature>
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        <location>
            <begin position="1203"/>
            <end position="1215"/>
        </location>
    </feature>
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        <location>
            <begin position="1216"/>
            <end position="1229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1229" mass="133857" checksum="38D073486E9578DA" modified="2023-11-08" version="1">MIGHLFSTRKSASAAHAQPATPLDSATEESHTRHLLYPETSTLYHPDGQPYPLHAAPLTPGSGHDGPLPEIDLDYPRDCRIIIAQDETPAMPKTILFDSKPAPPRAASPTMSNARARTFGGNNAATAATAGPAFGGLHARRSSLVTEPTAPPSPTVGGFTRIRARGSSISSMPNIDEHAQVRAKQARAKESTDLANVCLDCMFGNLAMNYRGNSNKIHIVPLDTKPADASLLSTSVQDAASSLGRAEGLRRRSHLAKSFTPANLPVDLPRCGSNDSVSKEPRRRTIFITRMFSVTIPEDDQDARTPTPQSSLPKGSGFPFPPTNNNSGASKPPYLSHIRKSPMYAITIILHLPISQAPPTLQPSSRGANGRNPAHLSTSAPGQDSLGSSLDSDRRAGWAFIDSNLGVESLLSSSLSSDVDDRVDVVGQHWDVIMRALTSLQHVVQERILAHFKSPDVASLPVLHQHRHQPSRTSLRDTPSHPSRKPLKLPSNALMMDKEIQTAAELTGNRVVSGMRIPRVMTGQGKWGVWREEARWLGRWAGRREQNFFFFNLLTAFLGNHTEWLNVLGPKWHRKRHREQQKATAGENLTIPNRTVIVSPDKMAARRLIFLLAAFLPPNAPTNSENASFLRPSTSASLRAYSQSPPTNMPPSRKQSLRRQINRRPKGKQSMTNMRLQPQYPQNGQQPSNDATVERSEPGMVETPDVARPVGHSRRSSAHSIRTGLVVASMSDGPVAAKSSIPTTSTASSQSAVPVALFTLPRTKSSGPMHDQRPESSDSLASANLINTLQKSGTWQTSTASCESSNTSRWSGFMNFWSGRRSSSTDQSDYLQTTDDGVGYRGQERPRNQLEQMVQELSMDRVFEGDGYEFPKADQDTDVGALPDVYPISGSSGVPASAARPIPERQRTFDTPLKLSVNEKDGVIDVDIPLPDFGSPLQSPLLGGCGSASSQHASSFGESSILSMPYCEPEQPVNAAGWLSQFHPDFAVQAIKPYQELERDIKRAMSAEPTPLNSAATPSLETGPLERWIDVCSALVADTSAFTIKRISLRRLVKLIPTPTYQPSAMTPGIAGMPPGRSQYGNPYTTGTVVPMMTEVHLAEKFIEEQIMDFDATLIDGVDRVLAQSGEVTRVNSTNSSRSSSRRGRRDTRADSDVNAIPHMEVSQTDCKSVIFDALESIVKNVTAERSGKGGELRDVDEKKSFRTTGQPPKTATDSSLKEGVRRWLTEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5H0</accession>
    <name>A0A9W8Y5H0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006657</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5H0"/>
    <dbReference type="OrthoDB" id="4486239at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="113" mass="12348" checksum="7646DCF79773E8DB" modified="2023-11-08" version="1">MACAIWAYEQLADAHARKRERSVVCFSGPQTPVPSKRPPRLPVNSPRLLLADPQVALNTPGRVQHASRPHITAGSTDSDPQLKTLVLKLTTQVEELTAMVSQLREQREASSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5U7</accession>
    <name>A0A9W8Y5U7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_007976</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5U7"/>
    <dbReference type="OrthoDB" id="1517790at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1660.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018714">
        <property type="entry name" value="DUF2237"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37466">
        <property type="entry name" value="SLR1628 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37466:SF1">
        <property type="entry name" value="SLR1628 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09996">
        <property type="entry name" value="DUF2237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4366340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="140" mass="15814" checksum="52FD314929664CC3" modified="2023-11-08" version="1">MSFLRNILKRSFLPTKTNTSTATREYAHVHDIQIKYTRAPAPITEEKPGDEDISASMERNGIVSNEFLDFSASRGNDLRQAGLTDGCKWCLCVSRWKEAFDARSGDNDKKVPQIVLKATNERALESVSMDDLRKFAVDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y669</accession>
    <name>A0A9W8Y669_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_007321</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y669"/>
    <dbReference type="OrthoDB" id="2096480at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050641">
        <property type="entry name" value="RIFMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43004:SF8">
        <property type="entry name" value="FAD-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43004">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21274">
        <property type="entry name" value="Rng_hyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="5">
        <location>
            <begin position="10"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4366796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="591" mass="66528" checksum="08CF597A16AE7D0F" modified="2023-11-08" version="1">MTSPKSDLYPVAIIGGGPVGLCLSILLSLRGIEHVLFERHPDTSIHPKSCGINQRTLEIFRILGIEGEVYAHCCPPDIAGRTAWYTSLGPRGKEITSRDAWGLGEYEEEYKKHSPSHYAILPQIRLEPILKKRAVQLNPSGVYYGCEVINHNQAKDYVTLIIRRHDSTEHHVEARFVLAADGGRSMTDRLGVPWLGERNILDMVSVHFKAPLRSLHPDPRNFMTWFISPEMGGSMKTGFLYQIGPWPHEPPKEEWVFCCAMTANDPENFDQENIIKRLRNTLKIEDLPVEVRSFTHWFVDARYAERYRVNRVILVGDAAHKIPPWGALGMNTGIQDAQNLVWKLDLTLKDEKRYDALLNTYEVERQPIGRRVAISSLHNMRSHAGCMDVALGLSPNKSVEDNMQSIAPAFDASHPDHAARRAEIERAQQILDLEFKAPGAEVGWFYPSVDTKNEGGKLHDGQLLPNGELNTAVYVPTTIPGHHVPHVWLQKDGRTCSVLDLIPLSKMLLLSRQPGWDALGDERVQYELIGHGGWQDMNDEWAKVSRVSESAAVLLRPDGIVAWRGQRQGLTSEAWKEIIDSVLHVRHAGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6Q4</accession>
    <name>A0A9W8Y6Q4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cyclopropane-fatty-acyl-phospholipid synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_006372</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFA/CMAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6Q4"/>
    <dbReference type="OrthoDB" id="8300214at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050723">
        <property type="entry name" value="CFA/CMAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003333">
        <property type="entry name" value="CMAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43667">
        <property type="entry name" value="CYCLOPROPANE-FATTY-ACYL-PHOSPHOLIPID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43667:SF2">
        <property type="entry name" value="FATTY ACID C-METHYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02353">
        <property type="entry name" value="CMAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003085">
        <property type="entry name" value="CMAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4368017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="50682" checksum="EC9DD1EA03793588" modified="2023-11-08" version="1">MAILSPITGAASTALSTLTSQAWAPLVSVCRNGTLSQLHQGIKYGKLVLFEGGSSKTSTVFGTDKEGLPIAFLDVHDEKFWVRLALFADMGFAESYMLGEFTTPDLTKFFELFILNRNYLSNGSTLTSAVSTGLAGLFRGTNNLKNARLNIAAHYDISNDMFAAFLSPDMTYSSPIWLPKSNPQSAHESLYDAQMRKLDRFIDNTHIKGSDHVLEIGTGWGSFAMRAVQRTGCRVTSVTLSIEQKELAEERIRAAGLSHAITVLLCDYRNLEIPESGPYDKIVSIEMLEAVGKQYLNTYFACIDKLLKKEGGIACFQCITMPDARYEAYAKSDDFIRRYIFPGGHLPAVSELVASIHSASSGALVVDSIENIGPHYAKALRLWREAFLDKWASKIKPLLIEEKRGQGMDDEGAEVFRRKWDYYFRYSEAGFLTKTLGDVIVTVGREGATQMMEDVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6Y5</accession>
    <name>A0A9W8Y6Y5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7514 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005954</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6Y5"/>
    <dbReference type="OrthoDB" id="5413703at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055936">
        <property type="entry name" value="DUF7514"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39611:SF1">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN DZ-HRGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39611">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN DZ-HRGP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24355">
        <property type="entry name" value="DUF7514"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7514" evidence="2">
        <location>
            <begin position="212"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="365"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="554"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="593" mass="66730" checksum="FF102DB7994A0CE4" modified="2023-11-08" version="1">MAYRDQPEPHQASYPTSYDGSYDSRAYYDGDSQYQNPTNGRSRASSSATSDGPQQPVQHPLKNAIGNAFEKSDAARVVDPDLIAQITAEVKKSVLDEIKMGVMAGATQPQPGPMSPQQYVPPSPASTSASFPPRDVYTPPSPKRTDFHSQSPHTADPLLRDPLLDSNDNTPTPRHERSAPINVPRERSSARPNPAVRMATDDYTPIEKMWQRLFESNGQPLPRLGEFLRGLALHLIEDYEPKKSLVISPSKMLKFYEEVKIQDEIYPWQTIFGKLSYTSLSKIYRDMRCQHHLIQEHPAEQPNIPALTPDGFQEWMTAMIQAYPDAEYERVAKAVLDMPISNADDRKERFPKELPRRMFPTAENLQAQQRCAAALSSEGVGPLRKAPTFPPPPPKSQSVPIPNLERERSPYVSQPESRPFESDDEMESTTVPIERQRKPYAATPGAGKTHDNDLNQSTYPEAAMNEQRRRAQSTASHGPWAPPPDPYQSRAGATANSRRPRSPSFSNYGTQSDPAFRDIPSSYYASNLHDDHDDSRRFSRDSDGKRQDRHRRSTAGTDGSFDSQPRSVYDDDEYRGRGGSNGYENRGYDSRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7R3</accession>
    <name>A0A9W8Y7R3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005364</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7R3"/>
    <dbReference type="OrthoDB" id="2119228at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01833">
        <property type="entry name" value="XynB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051532">
        <property type="entry name" value="Ester_Hydrolysis_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30383:SF2">
        <property type="entry name" value="CELLULOSE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30383">
        <property type="entry name" value="THIOESTERASE 1/PROTEASE 1/LYSOPHOSPHOLIPASE L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040883191" description="SGNH hydrolase-type esterase domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="SGNH hydrolase-type esterase" evidence="2">
        <location>
            <begin position="37"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="235" mass="24931" checksum="0A41EE64EFEF0396" modified="2023-11-08" version="1">MRFNFGSATLAALALLAVVTAAPAEIAERQSTVKIMALGDSITGSPGCWRAYLWQKLQQAGIKNTDFVGTLPAQGCGFQYDGENEGHGGFLAINIADQNQLPGWLSSTKPDIVMMHLGTNDVWNNRSPAEITTAFSKLVDQMRASKSSMKILVAKIIPMNPPNCGECGARVVALNSVIDSWAAGKNSSSSPITVVDTWTGFDTATMTGDGVHPNDVGNKKLAETWFAPLASAIQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7S0</accession>
    <name>A0A9W8Y7S0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytosolic endo-beta-N-acetylglucosaminidase TIM barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005641</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7S0"/>
    <dbReference type="OrthoDB" id="284473at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033925">
        <property type="term" value="F:mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032979">
        <property type="entry name" value="ENGase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005201">
        <property type="entry name" value="TIM_ENGase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13246:SF1">
        <property type="entry name" value="CYTOSOLIC ENDO-BETA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13246">
        <property type="entry name" value="ENDO BETA N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03644">
        <property type="entry name" value="Glyco_hydro_85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytosolic endo-beta-N-acetylglucosaminidase TIM barrel" evidence="1">
        <location>
            <begin position="103"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="704" mass="78765" checksum="667AA072684FE439" modified="2023-11-08" version="1">MANLLGWKDILRPIRDGYRHLFPTPDTGPTPEERCKQREKDRMNGFTYFDTFEQLEAWTEVESDPLQRANTPLLRRQPANKIASLDKSNIILIHDYAGNYHNYESVQGIAMDEEMYACEYLQYIDTFIYFSHKLVCIPPPTWTNTLHRNGVSTLGTVLIEPQTAGSEKLLQYTKNGNGMVFPMAKTLGDIARHYGFDGWLLNIEKPFPKADWRSDILESFLGQLRNYSDMEMRLVWYDALTASNKVSYQNALNSSNIAFASASGSLLTNYCWTETDAATSRQIASDNKIPHRDIFFGIDVWAQNASKLTHPRVTYPEKGGGGTNTGIAVTKVAELGLSAGIFAPAWSFEHFPHHGRAVEQAMWEAKSFSEDTECSCGNGSTRHQPNIDFPILRSAKEFPAGSESFFYADFSRAWCEHRVEGNNVVLGCHTRHSQLGAQSILPCPPDLSNNDEGIDLSRRLVDSDGRAMLIVEAQQKISLTADNHLEQLLPLYKFDMPANGFLHFMASCRKLLLSTTGASIGFYLKTTRGLLLLPIPENNSLQSIQTTVGMASHLTADDRLQELGIHLRGCTNDVGEVVQLLEIFELGILPESAVQKSQLFTISNIRVEHRGQGETTHARLCWSFNGTSDAEARVEGLPYSKVTGPVSYFNIQVAGLRLGRAYAMEHPLVQSLVEHFMGREMKVDITGIGFDGRQLVHSKTVLHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8C6</accession>
    <name>A0A9W8Y8C6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_004983</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8C6"/>
    <dbReference type="OrthoDB" id="5373744at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045138">
        <property type="entry name" value="MeCP2/MBD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15074:SF5">
        <property type="entry name" value="5-METHYLCYTOSINE G_T MISMATCH-SPECIFIC DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15074">
        <property type="entry name" value="METHYL-CPG-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="324"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="445"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="333"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="462"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="480"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4370465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="498" mass="55807" checksum="99796CE281AE842F" modified="2023-11-08" version="1">MVAIPRSLLQAIDGVPASAYSQYQTPRLSGAQAHDVRSFFKPLQNVLNSYNTVMDTSGSLAVATGYRSVARRLLDRLENVFARNISAEQCQCMMCFEQPLPEESAGVNWGEILELVSGRCELPAWPPYEDGPGAGLGISSSTLEAPCQRIDVDVPDQYREHYEKQSKKTKVAVQSWLSDQQENVPEDADDETLTFIMLTRLEQPQRPLFYALLWGLDKLPNPRTQKPDTGTPPYLAKASVALQRLYRLFAAPRDQLVVMYLIRHPDMHNMLATLAAVSKHEWEILVSGRFDGFLWSGADDMPELNSTPSRTASRNMSGHIPPTDGPQRFASPFSPNRFGTFSPSPYSRPGSARPGSVRPPGGAPVQIDEETEIAVLAEIEREIYLGMEAMEDAFEQLHNQAEQVRRRLRERAAALSMVAQQRRGSGIEVRTDTPANLRDADEGIDDLRSEIGPDDSASNVSHNRRRKAHRVRERRTPAPVEEESEEEIALAERIRRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8H9</accession>
    <name>A0A9W8Y8H9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase</fullName>
            <ecNumber evidence="6">2.1.3.2</ecNumber>
            <ecNumber evidence="5">3.5.1.2</ecNumber>
            <ecNumber evidence="17">6.3.4.16</ecNumber>
            <ecNumber evidence="4">6.3.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">URA2</name>
        <name evidence="27" type="ORF">N0V83_006692</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="27">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="22">Multifunctional protein that encodes the first 2 enzymatic activities of the de novo pyrimidine pathway: carbamoylphosphate synthetase (CPSase; EC 6.3.5.5) and aspartate transcarbamylase (ATCase; EC 2.1.3.2). The CPSase-function is accomplished in 2 steps, by a glutamine-dependent amidotransferase activity (GATase) that binds and cleaves glutamine to produce ammonia, followed by an ammonium-dependent carbamoyl phosphate synthetase, which reacts with the ammonia, hydrogencarbonate and ATP to form carbamoyl phosphate. The endogenously produced carbamoyl phosphate is sequestered and channeled to the ATCase active site. ATCase then catalyzes the formation of carbamoyl-L-aspartate from L-aspartate and carbamoyl phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">In the C-terminal section; belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">In the N-terminal section; belongs to the CarA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="27">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="6"/>
    <dbReference type="EC" id="3.5.1.2" evidence="5"/>
    <dbReference type="EC" id="6.3.4.16" evidence="17"/>
    <dbReference type="EC" id="6.3.5.5" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1924069at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005951">
        <property type="term" value="C:carbamoyl-phosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004087">
        <property type="term" value="F:carbamoyl-phosphate synthase (ammonia) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01423">
        <property type="entry name" value="MGS_CPS_I_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000002">
        <property type="entry name" value="Aspartate carbamoyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000036">
        <property type="entry name" value="Aspartate carbamoyltransferase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000025">
        <property type="entry name" value="Bifunctional pyrimidine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000005">
        <property type="entry name" value="CAD protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000004">
        <property type="entry name" value="Carbamoyl-phosphate synthase (glutamine-hydrolyzing)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.20:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1030.10:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1380:FF:000009">
        <property type="entry name" value="Carbamoyl-phosphate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="entry name" value="Carbamoyl-phosphate synthase small subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="entry name" value="Carbamoyl-phosphate synthetase, large subunit oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CPSase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CPSase_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058047">
        <property type="entry name" value="CPSase_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002032">
        <property type="entry name" value="PRK00856.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003671">
        <property type="entry name" value="PRK05294.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009455">
        <property type="entry name" value="PRK12815.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009475">
        <property type="entry name" value="PRK12838.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF5">
        <property type="entry name" value="CAD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25596">
        <property type="entry name" value="CPSase_L_D1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00101">
        <property type="entry name" value="ATCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="Carbamoyl phosphate synthetase, large subunit connection domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="Carbamoyl phosphate synthetase, small subunit N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 24" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10 24" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-grasp" evidence="25">
        <location>
            <begin position="567"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="25">
        <location>
            <begin position="1102"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="26">
        <location>
            <begin position="1359"/>
            <end position="1518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00043979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00043984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00058513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00081752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="KAJ4368336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1841" mass="203625" checksum="2DFAD0BAFF470F60" modified="2023-11-08" version="1">MAQGPDDSQNLALELQDGYVCFGRSFGAEKSIAGELVFQTGMVGYPESITDPSYRGQILVITFPLVGNYGVPSREEMDSLLKDIPAHFEAKEIHIAGLVVATYAEEEYSHHLATSSLGEWLKEQGVPAITGVDTRALTKRIREEGSMLGRLLQRTGPSPSSTALTNGAVDTAELTDGAVDDWRESFEQIEWRDPNQTNLVAEVSIKEPKLYSPDPANALQHPSGRPVRVVTVDVGLKYNQLRCLVRRGVEVLQVPWDYDFPQLAGKDYDGLFISNGPGDPAMMEPTVKHIKAAMDEARTPIFGICLGHQLLARAAGADTLKMKFGNRGHNIPCTSLLTGKCHITSQNHGYAVDSKTLPHGWEELFVNANDGSNEGIRHVRRPYFSVQFHPEHTPGPRDTEYLFDVFISTIQKAINSSEVMSQAVEFPGGTFEENKKASLRVDVKKVLVLGSGGLSIGQAGEFDYSGSQAIKALKEEGIYTILINPNIATIQTSKGLADKVYFLPVNADFVRKVIQKERPDGIYVTFGGQTALQVGIQLKDEFEGLGVKVLGTPIDTIITTEDRELFARSMESIGEKCARSASANNVEEAMRAVKDIGFPVIVRAAYALGGLGSGFADDEGQLLDLCNKAFAASPQVLIERSMKGWKEIEYEVVRDARDNCITVCNMENFDPLGIHTGDSIVVAPSQTLSDEDYNMLRTTAVNVIRHLGVVGECNIQYALNPFSREYCIIEVNARLSRSSALASKATGYPLAFVAAKLGLNIPLNEISNSVTRSTCACFEPSLDYVVVKIPRWDLKKFTRVSTLLGSSMKSVGEVMSIGRTFEEAIQKAIREIDPSNLGFNETPALMEIDQELQTPSDQRLFAIANAMKAGYSVDKIWEMTNIDKWFLSRLKGLSNFENSMSSYNTASITLPLLKKAKALGYSDRQLAKFWASNELAVRRVRVDAGIFPVVKQIDTVAAEFPAVTNYLYLTYNGTEHDVSFHDKGVMVLGSGVYRIGSSVEFDWCSVRAIRTLREQGFKTVMLNYNPETVSTDYDEADRLYFENISLETVLDIYTLESSSGVIISMGGQTPNNIALPLHRMNVNILGTSPEMIDTAENRYKFSRMLDRISVDQPAWKELTSIEEATEFCDRVSYPVLVRPSYVLSGAAMNTVYSKHDLANYLNQAADVSKEHPVVITKYIENAKEIEMDAVARNGTMIGHFISEHVENAGVHSGDATLILPPQDLDPETVRQIEDATRKIGNALNITGPYNIQFIAKDNEIKVIECNVRASRSFPFVSKVMGVDLIEMATKAMAGLPITEYPPVNIPAEYVGVKVPQFSFSRLSGADPVLGVEMASTGEVACFGRTKYEAYIKGLIATGFRLPKKNILLSIGSFKDKLEMLPSIERLHKMGYNLFATAGTADYIQEHGIPVKFLEVLPQGEHDEQKSEYSLTQHLSNNLIDLYINLPSSNRFRRPANYMSRGYRTRRMAVDYQTPLVTNVKNAKLLIEAIARHYDLEISKVDFQEFAAAPGSHEQSSLEMSRLNGSLSLNQLLAKSPFKRQHVTSVKQFSRADLHLLFTIAQEMRLGAQRQGCLDILKGRVLCTMFFEPSTRTSASFDAAMKRLGGEVVVINESVSSTQKGESIADTIRTLGCYGDAIILRHPAEESVDIATKFSDVPIINAGNGSREHPTQALLDIFTIREELGTVNGLNITFVGDLKYGRTVHSLCELLSHYDVRVNLVSPKSLSMPSGVRDALKSRGQLNQVSNELTADIIASSDVLYCTRVQKERFPDVATYEAVKDAFVIDNKVLKGAKANMIVMHPLPRNNEIHEEVDFDPRAAYFRQMRYGLYTRMALLAMVMAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y913</accession>
    <name>A0A9W8Y913_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_005038</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y913"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000023">
        <property type="term" value="P:maltose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000149">
        <property type="entry name" value="MFS transporter, SP family, general alpha glucoside:H+ symporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF5">
        <property type="entry name" value="ALPHA-GLUCOSIDES PERMEASE MPH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0462">Maltose metabolism</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="349"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="378"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="406"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="446"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="58"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="536"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="536"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4370517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="559" mass="61483" checksum="2248F38405F30B90" modified="2023-11-08" version="1">MSRRNSSVVAVDPEQARRISVAVPDIQQVTHDANKATQSEQNMSLREGLRLYPKAVAWSVLLSAAIIMEGFDKVLIANLMAVPAFKRKFGAPLPNGTYEVTAAWQAGLTNGAFVGELSGLLINGIIADRIGYKKTMIGALFAVNLFIFVVFFAQNTVMLLCGLILCGIPWGVFQTLTCTYAAEVCPVPLRPILTTYVNLCWVIGQFLASGVLKGVAERSDQWAYRIPYALQWLWPVPLMIGISFAPESPWWLVRKDRDDDAKKMLMRLTSPEKHPNFNADETIAMMRHTNELEKAVSEGTSYLDCFKGTDLRRTEIAAMTWFTQAFCGAALIGYSTYFFQQAGLNDSDAFSMSLGQYAIGGVGVFVAWALIPRVGRRTLYVCGDIVMVILLVIIGGLGVIPEGHIGAQWGIGAMLLLFAAAYNCTVGPVCYAIVAEIPSTRLRQKTVVLARSFYNLASIVGNILTPRMLNPGAWHWGAKAGFFYGGTCFGCLVWAIFRLPEPKGRTFAELDILFEKRVSAWKFKQTVVESFHVSDSESDFGEKKGASAHLEQVSSKQVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y988</accession>
    <name>A0A9W8Y988_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NodB homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006032</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y988"/>
    <dbReference type="OrthoDB" id="3162524at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10938">
        <property type="entry name" value="CE4_HpPgdA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037950">
        <property type="entry name" value="PgdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47561">
        <property type="entry name" value="POLYSACCHARIDE DEACETYLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G05030)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47561:SF1">
        <property type="entry name" value="POLYSACCHARIDE DEACETYLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G05030)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NodB homology" evidence="2">
        <location>
            <begin position="29"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="308" mass="35350" checksum="4D304BC788678F24" modified="2023-11-08" version="1">MGKKRVLISYGVDVDAVSGWLGSYGGEDSTSDISRGLFAGTVGTQRLLRLFDKYKIKTTWFIPGHSLETFPEDMAAVRDAGHEIGLHGYSHENPKDMTLEQQRDVLDKTYRMLTEFAGKPPRGSVAPWWETSQEGAELLLSYGIEYDHSMSHHDCQPYYLRTGDTWTKIDYSKKAVDWMKPLVPGRQTGLVEIPANWYIDDLPPMMFIKASANSHGFVNARDVEDLWRDHFDYFYREYDEFIFPMTIHPDVSGRPHVLLMHERLIKHFLEHEGVEFVTMEQMADEFKKKNPPPEGALMPAEAGLKLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9C6</accession>
    <name>A0A9W8Y9C6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004524</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9C6"/>
    <dbReference type="OrthoDB" id="74545at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37015">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37015:SF2">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="378"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="139"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="394"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4371307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="954" mass="108414" checksum="1C5BDDA344F744A4" modified="2023-11-08" version="1">MDPMPPFTVTPVLRAAAELKIEELNRAKESFKRRYHLDDCRSAESNVIKRISELLEDIRKFDRYLDDDDDLAIMARFMEQARDDKSISELKLSKFEEQLRDKLTKHLNRMEVSSLHVELMKEVMEAQPASDSAAAKLEKVTLDDDFEMVENELEEALEEFEEATFTAKDVHIDAIEAYLSSLIVGDTADKQLERLRRDMRSFGEGLVDNGREVEQDDLMWIIMELLKNDLISPEKKATLESYLQSPIALRELVACLNMQSFHHWRYKDAHKGLPITARQNAEGQYCITIEEDIVDMLFLNCIAIGWASKLKECLQNFCNRSGSFATKSLSAEEKHKREYYLSTFRTTVKPKASTCSICHPSYPPMSCPPPPPIIPYPIAPFPRKKDKHRHSLDGPPPPPPPPPPADSLAEERRQNYYRTFLMSRIPLLDGCTPKVTCPQEVQAKLIKTLAVEAKVREAIDGKAHAFAPRFDASSLPHKTILTVLKFLGVPQTFLDFFTRFLEARLNIGPAVRGTPDRILQRACGVPIGHGLEMLFSEAILFFLELSVFQKTESYMYRLYDKCYFVGTEEQQTKALQETTKFASTMGLSFRMGGGKMLIGCLTMDTLGSFSSPAMASFEIDHPTVLAYANRVKVQLDACTTVLDWVRIWNRTIGTYASHLFGPLSATVFGKAHLEDVKGVYNTVFSIIFNGGNLTAHVKTLLNTHIKRGLSDPPFALEALIYLPQAYGGLGVKNPFVTFNLAFNMRDGNPWDGNFLGLDDYFKREEAYYKRAAETYALCTPEQRLAKIEDMSSNNTFSTSNDKDQIHAALGLPDRDLATFMTRPELFAHRERISYPMLDSPPFPHPFKTGANPMLLDVYRDLLYEPSDTPPISSRVLDDVRRLAGTGDMKPWRKLSEEDKWMLQLYGDECFERYGMLEIWWREGVPGEVYKAVRGHVWSDGDDEDSDASSDYSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9M6</accession>
    <name>A0A9W8Y9M6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_004689</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9M6"/>
    <dbReference type="OrthoDB" id="272778at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066:SF21">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4371472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="279" mass="30934" checksum="7BAC3ACA4AE7C917" modified="2023-11-08" version="1">MLSSGFTNAPVSQFLVFSTVIGALLATITDTRYYLHIQVVPHVWNYGQFWRFATWQTCFTNSTEVLFAVLTFYQLRVIERLWGSRKFASFLLATLPYTTLLPPLLLTFVLRPLTFGRINYLPAGPTSILFALLANYYAAIPYTYRYKISPWAQSSSQSNTQAISTLWSRSLTLTSKSLSYLPPLQLALSQFPGSLLAAAVGWVVGTAYRRDLLPGAARWRVPGWMVGEEKQKGFEGLRARLDAEREREGGASGLATGILAGETGKGGVWQGGGRWGNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9R1</accession>
    <name>A0A9W8Y9R1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9R1"/>
    <dbReference type="OrthoDB" id="3793928at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="291"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="311"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="332" mass="37676" checksum="E8A1FB7C128F65FD" modified="2023-11-08" version="1">MTAESPTTESPTNSELPFGWNDHTFDDIHKVLLEKTYTHFVVDQDGFWYCPKCRWKAQYGHRWAFKVRGYAKLPEDALSLAVHASYGGDHPNGLYFKNPAKVVQCQNHWNTGLSGSRCTFNREKVEGHRDEAMEKEHDQGGFLGFCPQCSVTPDVLERNPAFNAYVQSEQVNKKGVELLKQHVHIFALHNEDPWFGKPRWMRQFWYDYTEESLKCPTKHFGEGGGEGKEDTPCPGRYGGPVVFYNRELEEELGQNTEMVETGQSPDESEMNTDNKTKVIYSVEWTPDETSWEGSESSSGSEKAPPMDFDDSTTSSSEWSSYGQQSPGGYASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9W3</accession>
    <name>A0A9W8Y9W3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA cytidine acetyltransferase</fullName>
            <ecNumber evidence="9">2.3.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">18S rRNA cytosine acetyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 15" type="primary">NAT10</name>
        <name evidence="15" type="ORF">N0V83_003965</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">RNA cytidine acetyltransferase with specificity toward both 18S rRNA and tRNAs. Catalyzes the formation of N(4)-acetylcytidine (ac4C) in 18S rRNA. Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis. Catalyzes the formation of ac4C in serine and leucine tRNAs. Requires the tRNA-binding adapter protein TAN1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a cytidine in 18S rRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in 18S rRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51424"/>
            <dbReference type="Rhea" id="RHEA-COMP:13575"/>
            <dbReference type="Rhea" id="RHEA-COMP:13576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a cytidine in tRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in tRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53876"/>
            <dbReference type="Rhea" id="RHEA-COMP:13670"/>
            <dbReference type="Rhea" id="RHEA-COMP:13671"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with TAN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the RNA cytidine acetyltransferase family. NAT10 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9W3"/>
    <dbReference type="OrthoDB" id="10067491at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990883">
        <property type="term" value="F:18S rRNA cytidine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904812">
        <property type="term" value="P:rRNA acetylation involved in maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051391">
        <property type="term" value="P:tRNA acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11040:FF:000002">
        <property type="entry name" value="RNA cytidine acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03211">
        <property type="entry name" value="RNA_acetyltr_Nat10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033688">
        <property type="entry name" value="NAT10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007807">
        <property type="entry name" value="TcmA/NAT10_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032672">
        <property type="entry name" value="TmcA/NAT10/Kre33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013562">
        <property type="entry name" value="TmcA/NAT10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027992">
        <property type="entry name" value="tRNA_bind_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925">
        <property type="entry name" value="N-ACETYLTRANSFERASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925:SF5">
        <property type="entry name" value="RNA CYTIDINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13718">
        <property type="entry name" value="GNAT_acetyltr_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05127">
        <property type="entry name" value="NAT10_TcmA_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08351">
        <property type="entry name" value="TmcA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13725">
        <property type="entry name" value="tRNA_bind_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TmcA/NAT10 N-terminal" evidence="12">
        <location>
            <begin position="9"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="TcmA/NAT10 helicase" evidence="11">
        <location>
            <begin position="283"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="13">
        <location>
            <begin position="539"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="Possible tRNA binding" evidence="14">
        <location>
            <begin position="775"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="435"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1016"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="447"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="288"/>
            <end position="297"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="479"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="638"/>
            <end position="640"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="645"/>
            <end position="651"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="739"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4372191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1067" mass="119019" checksum="1663C2A93FA1293B" modified="2023-11-08" version="1">MPKKAIDSRIPALIRNGAQEKKRSFFVVVGDRQKDVIVNLYHILLNVDVKLNKSVLWAYKNKLLGFSSHRKKREKKIKNELKRGIRDIDTEDPFELFVSTQNIRYVYYKETDKILGNTYGMCILQDFEALTPNLLARTIETVEGGGLVILLLKGMSSLKQLYTLSMDIHSRYRTEAHSDVVARFNERFILSLGKCDSCLVVDDELNVLPISGGKHVKQLPPPDPEMEGKTPKAKELVEIKDSLADSPPVGDLIKLAKTVDQAKALLTFVDAIVEKTLQSTVTLTAARGRGKSAALGVAVAAAVAHGYSNIYITSPSPENLKTLFEFVLKGFDSLGYVDHQDYSIMQSTQPELNKAIVRINLHRQHRQVIQYIKPEDSHQLGQAELLVIDEAAAIPLPLVRKLMGSYLVFMASTINGYEGTGRSLSLKLIQQLREQSRGRGSNGSTHVVDRSTGKETRDGTETSMAGRSLREITLSEPIRYAQGDSVERWLNDLLCLDATLPRSKMNTQACPHPSECQLLHVNRDTLFSYNPAAEKFLQKMMALYVASHYKNTPNDLQLMSDAPAHQLFVLTAPAEENKLPEPLCVIQVALEGQISRESVLNSLSRGQRAGGDLIPWIVSQQFQDENFASLSGARVVRIATNPDYVNMGYGSRALELLVEFYDGKLASLSESEPKEVEQMRRLTEADLENASLLQDNVRVREANEMPPLFARLPELKAPQLDYVGVSYGLTSQLHKFWKRASFVPVYLRQTPNDLTGEHTCIMLRSLETTSSDGSWVAAFAKDFQKRFLSLLSYQFRTFTWETSLSIEESASAGSKLDAELAPRGISKVELDALFTPFDLKRLDSYANSMLDYHVILDMLPSIAQLYFTGRLKGQVKLSGIQTAILLAIGLQRKEFADIEKEMGLHSSQLMAMFVKIIRKAATAFRSIVEGAVEETMPEAMQTEENGVDGEADESTQQRFKPLEKDLEEELKEGGDEILAEERERARSLIDALPLHKYEIGAGAADWEDAERAISKAATKGGSSSMTVAVKTGEKKRKVGEAVEEAYKEAEKFKEGGKKKKHKSSKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9Y9</accession>
    <name>A0A9W8Y9Y9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Asl1-like glycosyl hydrolase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003995</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9Y9"/>
    <dbReference type="OrthoDB" id="5985073at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071966">
        <property type="term" value="P:fungal-type cell wall polysaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053183">
        <property type="entry name" value="ASL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024655">
        <property type="entry name" value="Asl1_glyco_hydro_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34154">
        <property type="entry name" value="ALKALI-SENSITIVE LINKAGE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34154:SF10">
        <property type="entry name" value="ASL1-LIKE GLYCOSYL HYDROLASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11790">
        <property type="entry name" value="Glyco_hydro_cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040728953" description="Asl1-like glycosyl hydrolase catalytic domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Asl1-like glycosyl hydrolase catalytic" evidence="2">
        <location>
            <begin position="48"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="272" mass="29242" checksum="69CC572430F02646" modified="2023-11-08" version="1">MFKSAFVLTLAGLASLMEASPLKARGGGKRGLAFPKEYSGTAGSTYTHNFASASKVTWLYDWEAVIDGTPLGLEFVPLLHSNEAWCADGWAANVAAAQKQYSVSHVLGFNEPDQVGGGGTNMAVSDAVAAYKQYIQPLAKQGLRLGSPAVTNGAGANVGINWLKQFISGCGSDCTIDFVVAHYYAWDHADDFKNYLTTFHQTFNKPVWVTEFGVTSGDADAFLKEVLPWLDAQDWIERYAYHMVAPATSGSQWLINEAGNGLSSTGQVYAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAF2</accession>
    <name>A0A9W8YAF2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003955</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAF2"/>
    <dbReference type="OrthoDB" id="298012at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43333">
        <property type="entry name" value="2-HACID_DH_C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43333:SF1">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE NAD-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="20584" checksum="0E78AE35AB8488EF" modified="2023-11-08" version="1">MGGGEDLPSRNASKEKEIVVCVLPWPEEAAKRGIDDLKKEYKDAEVHYYNSKFENGKMHPIDIPEDLLKRASYLATLFWLPSSASAIPNVKAIQFFSAGTNHVAKHPIYTDSKIPLCSANGVHGPQIAEWVVMMDLVQSHNYVNLWEQQKKKEWKQSMGMSVSDRVGKRVGILGYGSIGRQGKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAH3</accession>
    <name>A0A9W8YAH3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAH3"/>
    <dbReference type="OrthoDB" id="2537432at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053203">
        <property type="entry name" value="Cisplatin_resist-associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022024">
        <property type="entry name" value="DUF3602"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34693:SF3">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34693">
        <property type="entry name" value="PROTEIN PAR32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12223">
        <property type="entry name" value="DUF3602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="145" mass="15279" checksum="1A7BC103D02AB6A6" modified="2023-11-08" version="1">MSGQPIHSTGRGGAGNIGPDANLYTDGGLVREGVQGESVDGEFSTGRGGAGNIGKSPRIGPQTTEGRRSVDLVPEINAREPQDEFHTGRGGAGNVYKEKYGGHSHSRSPDGRKPGIGEKIKEKLHLDKKDKEHSPSPLAQDETVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAI0</accession>
    <name>A0A9W8YAI0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_004352</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAI0"/>
    <dbReference type="OrthoDB" id="5422579at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4371136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="460" mass="53317" checksum="6703F92F1E04AF4A" modified="2023-11-08" version="1">MPLSIPYLPPEVHHVIAQYIDRFDLPNYRLANKAFAEIGTPELFQNITFHYSSASLARLTAIKQSEHLQKFVKVLTWDTNSWFLCGVKFFDEWQRYFNEKARLFTKTYQPKCEHDHGIKLSKIAEDRQQWKNYVVNRPDENETWCYCISHDFLADFSNLSKVFMVNGALVPDHRGLKKTGIRVLLSEDEPAIFARGVCLDTVGSNGSFDQEPARLAFRNMYEHFGPYLKKLRVNALDWQSFFQYPRIPPMGAFDTLTSLHLQLTARSDCEYMSERFGVQYVRSQIREGNVAEFLARLLRLESLRLDLEDHYAHDGSDGRRVYKAPSTVADIFPLDHTWPNLRKLSLHHLDTTPHALLKLLGTHSATLKDMRLQHISLEVEESNNRSWHQVLQKIRETLHLERASLSGLLISENKDDFWDLDDDEALGLATAKYLVEGGECPVPAVRLDLKWNDCVDTFIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAX0</accession>
    <name>A0A9W8YAX0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF866-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_003136</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0587 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAX0"/>
    <dbReference type="OrthoDB" id="10248838at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008584">
        <property type="entry name" value="CXXC_Zn-binding_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12857">
        <property type="entry name" value="CXXC MOTIF CONTAINING ZINC BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12857:SF0">
        <property type="entry name" value="CXXC MOTIF CONTAINING ZINC BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05907">
        <property type="entry name" value="CXXC_Zn-b_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141678">
        <property type="entry name" value="MAL13P1.257-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4372845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="18155" checksum="1A6D58FF023458FC" modified="2023-11-08" version="1">MVLALALKAELNGVTDLRPQDTEDAPFYYTFKVQCTSCRETHPNLVSVSRFEQNEMSGSRGEANFVWKCKNCKREHSANIKAAPATYEQSDPPKTVNILEFDCRGLEFTEFKAEGLFNATGAESGTKFSGIELHEGEWFDYDEKAGEEVSITGIQWEIRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB16</accession>
    <name>A0A9W8YB16_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CAP-Gly domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_003966</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB16"/>
    <dbReference type="OrthoDB" id="2130750at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032108">
        <property type="entry name" value="CLIP1_ZNF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF83">
        <property type="entry name" value="TIP ELONGATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16641">
        <property type="entry name" value="CLIP1_ZNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CAP-Gly" evidence="9">
        <location>
            <begin position="80"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="129"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="537"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="615"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="729"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="418"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="149"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="241"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="560"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="623"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="740"/>
            <end position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4372192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="798" mass="86549" checksum="A54C280E3173D035" modified="2023-11-08" version="1">MSFQTPRRLNPLRPSLNPTASSPNLSLTAQQQFARKASLNALVGGASPPANRMGADGRDIGVGDAVDVPGGMHGVVKFIGSVNGKKGVFAGVELSRQYAARGKNDGEVEGTQYFKTSIPGSGIFLPVHRASKRSSADSSDFPPTPTTPSYHNNFSLSTTEKPNFSPDTPSVSMPKFSQSVGPGARAPSPQFKPKGRPSLPRPESPYRQKPNLTATPARNGGITPSGFSKSAIGAPPRFASPAPPRFNSPAPPRAAPLQKPRTPGPSRPYSRNNSRLGQHDTIDEDSETTPVGAVRTSNDSMSSFKNRRPGSRPGSNHTEEVQRLTKLLEERDKQLKDQAASLSEMEASLSEIQNLIPEDGMDFGSSQSHGEPNDVGQLRVLLREKNDKIAMLTSEFDAHRSDFRSTIDTLELASTETERVYEKKVEELQEELREYQSRSEDVETVAMQLKQLEELVQELEEGLEDARRGEAEARGEVEFLRGEVERGRSELRREREKAAAALKGANTMVDAPHTPGQREVEQRDDEIRGLKAIIHSLSSGGAEDHGRSPLSRQGSSVNPEELRSAKANADRLEREKEELRGLVERKAYREEELERELEKLKSQLGLAEQRESVISNGLSEHTATQEKRSSGRDSKGTVVTWRDSPRREVPPVLTPMAESDGRSSANSSLLWCEICETGGHDILNCSAMGGNDVPTVSSHPNKENGTTTTTRNGKDVVIEGLRNLSVSTADYPRPSVLSPSKPPGSAPTAPLPIPAAFNHSSTSLVPPKGAAVADPDKWCALCESEGHDVTACPNEDAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB27</accession>
    <name>A0A9W8YB27_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-nitropropane dioxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_004998</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB27"/>
    <dbReference type="OrthoDB" id="10265891at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018580">
        <property type="term" value="F:nitronate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04730">
        <property type="entry name" value="NPD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004136">
        <property type="entry name" value="NMO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32332">
        <property type="entry name" value="2-NITROPROPANE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32332:SF36">
        <property type="entry name" value="2-NITROPROPANE DIOXYGENASE FAMILY, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G07940)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03060">
        <property type="entry name" value="NMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4370478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="36697" checksum="2A7DC208284695A1" modified="2023-11-08" version="1">MSTPSLQTPLSKLLGIQYPIILAGMARTSGGPLAAAVSNAGGLGVIGGLGYTPAQLQSIIDDLKSNLRDKSLPFGVDLALPQVGGSARKTNHDYTHGQLDDLIEVTIQNGAKLFVSAVGVPPARTIKRLHEAGILIMNMVGHPKHAKKALDAGVDIVCAQGGEGGGHTGDIANSILIPSVVDVARKYKSPLTGETAIVVAAGGIYNGRSLASSLMQGAQGVWVGTRFVASTEAGCSQMHKENVVSADFTDTGRTLVVSGRPLRVRYNDYIKGWHEREDEIKKLTNAGIVPLAKDMDDGKDVDIPFLMGQVSGVITEIKPAKEIVDEMVQEAVDMLRLGQTYIGGPSSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB28</accession>
    <name>A0A9W8YB28_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transcriptional activator haa1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">HAA1</name>
        <name evidence="10" type="ORF">N0V83_004653</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB28"/>
    <dbReference type="OrthoDB" id="5600085at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.430.10:FF:000001">
        <property type="entry name" value="Copper fist DNA-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.430.10">
        <property type="entry name" value="Copper fist DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051763">
        <property type="entry name" value="Copper_Homeo_Regul"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001083">
        <property type="entry name" value="Cu_fist_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036395">
        <property type="entry name" value="Cu_fist_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28088">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28088:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00649">
        <property type="entry name" value="Copper-fist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00617">
        <property type="entry name" value="COPPERFIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM01090">
        <property type="entry name" value="Copper-fist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00412">
        <property type="entry name" value="Cu_FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57879">
        <property type="entry name" value="Zinc domain conserved in yeast copper-regulated transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50073">
        <property type="entry name" value="COPPER_FIST_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Copper-fist" evidence="9">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="54"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="330"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="252"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="331"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4371436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="489" mass="52661" checksum="501DB1E62768EA0C" modified="2023-11-08" version="1">MPVIDGAKWACSSCIKGHRVSGCTHNDRELHHINPKGRPVKQCEHCRGARKSKSHHAKCDCGDKKDKDKHKDKGDAKGMTTTGTADLDESNDALAHDGGCQCHSGAKCICGTIKEELKVDTSRQTLHDARAKPKLMTTHSESHLTVFANGHHKPCHRNNNTAHVSGAPYKIPRPHTLHGHSAFAALSQSNNSYSKPEVPATRSMDTLSLSNNDFYAMFGSTPRSVDNMPITNMANSVDTLPFQDALFTSQSSVFGQDGQSPDSNSDTFSSQQWPWTTTTATPINGTFGFGSLSTSPSQDCLPNLDNDWSIPSAGFNPLWSAGDLPLDPSKFSDSLTQPISHSGESKQSGPGLTAASSAHSEIGDPNIFGDVEYKPPQSAASESLFWEDSPVYRFNTSAPSENIAAPVTLPASTSMPNIPELEPTFAKSLSAAPTTMTSTASNDYTETAAIAMPTNYEDVVPIDPWPLDQNNGAFGALNNFELSYSNNWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBB1</accession>
    <name>A0A9W8YBB1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBB1"/>
    <dbReference type="OrthoDB" id="3497702at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040829134" evidence="1">
        <location>
            <begin position="19"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="132" mass="13742" checksum="68EEDD921A9D37D7" modified="2023-11-08" version="1">MKTSIIASTFALFSGALAIPTLSARQAPQFFTFSLSNDITGANAARSVVVNGGPVTLGGLFSWSPLVKDGRVVATSAQNINPVGGAVLCIFTDPATGNVFKLNDKVTFADLDGNNHAAVQTDVTDFTFQCEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBG7</accession>
    <name>A0A9W8YBG7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Importin-95</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Karyopherin-95</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">kap95</name>
        <name evidence="11" type="ORF">N0V83_003467</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the importin beta family. Importin beta-1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBG7"/>
    <dbReference type="OrthoDB" id="10263328at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000027">
        <property type="entry name" value="Importin subunit beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="10">
        <location>
            <begin position="21"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="9">
        <location>
            <begin position="411"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00079884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4373175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="872" mass="96073" checksum="947FED157BC33C42" modified="2023-11-08" version="1">MDINQVLEGTYSADASIRNSAEQQLTQAADSNFPQYLTVLGGELANEQAQPQIRQAAALALKNAFTAREYARLRQVQERWLSLDPQIKQEVKAMALRTLSTQNKGVGSAAAQFIASVAAIEIPRNQWPELMGTLVENVGNGSDSQKQASLTTIGFVCDTDDGELREALAHHSNAILTAVVQGARKEETNNDVRVAAINALSDSIEFVRSNFDNEGERNYIMQVICEATQADDSRIQQGSYGCLNRIMGLYYEKMRFYMEKALFGLTIQGMKSDEEDVAKLAVEFWCTVCEEEIAIEDDNSQSQAEGSTELREYFNFARVATQEVVPVLLELLAKQDEDADDNEYNTSRAAYQCLQLWAQCVGSGVMPPVLAFIEKFIRSEDWHYRDASVSAFGAIMEGPEESVLDPIVKQALPTLIGMMDDQNIHVKDSAAYALGRICEAVPSALDAQQHLPPLIGSLFNGLASSPKMAASCCWSLMNLADRFAGEPGCQTNPLSPHFAQSVQHLLTVTERGDADNQLRTAAYEVLNSFVNNAAGDSVPLVNELSNVILERLEKSMALQQQIVNVEDKLTLEEMQTSLASVVMAIIQRLEGDIRPQADRIMQILLSLLSSLPPKSSVPDTVFAAIGSIATALEDDFQKYMEAFSPFLYNALNNQEEPALCSMAIGLVADITRSLGENVQVYCDAFMNSLLNNLRSPALGNQLKPAILQCFGDIAHAIHGAFENYLPVVAQVLQQAGQVTLTTEGNYEMIDYITSLREGIMDAWDGCIVAMKTSNKTQLIVPYLDSIFDLLRTIQGDSNRTEALLRSSCGVIGDLADAFPTGEFREYFRHDFLTQMAREARSNADFSSRTRDTARWAREQIKRQIGGNTGVMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBK1</accession>
    <name>A0A9W8YBK1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">fumarate reductase (NADH)</fullName>
            <ecNumber evidence="7">1.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">NADH-dependent fumarate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">OSM2</name>
        <name evidence="11" type="ORF">N0V83_004273</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>succinate + NAD(+) = fumarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18281"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.3.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.6" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBK1"/>
    <dbReference type="OrthoDB" id="10252157at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016156">
        <property type="term" value="F:fumarate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000007">
        <property type="entry name" value="NADH-dependent fumarate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050315">
        <property type="entry name" value="FAD-oxidoreductase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010960">
        <property type="entry name" value="Flavocytochrome_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01813">
        <property type="entry name" value="flavo_cyto_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43400">
        <property type="entry name" value="FUMARATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43400:SF1">
        <property type="entry name" value="FUMARATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="10">
        <location>
            <begin position="554"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="492"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="505"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="531"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00077246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4372499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="638" mass="69591" checksum="29959F95ADE8C936" modified="2023-11-08" version="1">MSQPQRVIVVGAGLSGLSAAHTIYLAGGNVVLLDKNNFMGGNSTKATSGINGALTRTQVDHKIGDSVKQFYDDTLKSARDKARPDLIKVLTYKSAAAVEWLMDVFNLDLTLVSRLGGHSQPRTHRGHDAKFPGMAITYALMQRFEELAEQEPERVQLVKKAKVTKVNTEANRATGVTYLFNGEETTVEGPVILATGGYAADFTDDSLLKKWRPDTFDLSTTNGAHATGDGHKMLMKIGAQGIDMDKVQVHPTGLVDPKDPTAKTKFLAAEALRGEGGILLNSKGKRFCDDLGHRDYVSGMMWEQKEKSNWPIRLVLNSKASNVLDFHTRHYSGRGLMKKMTGKELAKEIGVSDKELQEEFKSYNAIAKGEKKDTWGKKYFHNLPFDIDDTFHVAHMEPVLHFTMGGIEINDQAQCLNADGKPFDGLYVCGELAGGVHGANRLGGSSLLGCVVYGRVAGESASKYLFQQALNSAGGSAVSRLGQISLHIDPSQPGKVSVEWGNGASGSSQSAQSQDKVQQQKQESAGPVMKSDGDSKDPGKVSKPNEPKKFTIPDKEFSLEEVAKHNKKDDLWIAVKGIVMDVTNWTDEHPGGPQALFSHMGKDASEEFEMLHDDEVIPKYAPEIVIGRVKGQEVTLEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC84</accession>
    <name>A0A9W8YC84_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fungal N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003667</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC84"/>
    <dbReference type="OrthoDB" id="3045089at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38886:SF1">
        <property type="entry name" value="NACHT-NTPASE AND P-LOOP NTPASES N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38886">
        <property type="entry name" value="SESA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4373372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="263" mass="29959" checksum="318AB1A27E33CB4F" modified="2023-11-08" version="1">MVIPFGVSAGDFIAGLKLFKDAIDSLSDVRGARADYIELTQTLSALTTALDAASQFDGAVHQAAITVVLQGCRECVKDFLTDIAKFELLKNKSVTVRRLKTGLKKVQWALCKKEDVLNFKGHLETHLGLLPDEQRTMFRMLEEALAERTSFERQMEQLTVQNRILETHLLEIKAAVQQQREIPPQVLLRRPVILIDAFEENRPPFHLVFINSFEALFAVFMIRFKDKGGGAVDRIRRQLFDMFEQSRQKRIDINGPWANAFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCH6</accession>
    <name>A0A9W8YCH6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PAN2-PAN3 deadenylation complex subunit PAN3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">PAB1P-dependent poly(A)-specific ribonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Poly(A)-nuclease deadenylation complex subunit 3</fullName>
            <shortName evidence="10">PAN deadenylation complex subunit 3</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 14" type="primary">PAN3</name>
        <name evidence="14" type="ORF">N0V83_003528</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein PAB1. PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. May also be involved in post-transcriptional maturation of mRNA poly(A) tails. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and with PAB1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer. Forms a heterotrimer with a catalytic subunit PAN2 to form the poly(A)-nuclease (PAN) deadenylation complex. Interacts (via PAM-2 motif) with poly(A)-binding protein PAB1 (via PABC domain), conferring substrate specificity of the enzyme complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">Contains a pseudokinase domain. The protein kinase domain is predicted to be catalytically inactive because some of the residues important for catalytic activity are substituted and it lacks the equivalent of the binding site for a peptide substrate. However, it has retained an ATP-binding site and ATP-binding is required for mRNA degradation, stimulating the activity of the PAN2 nuclease in vitro. The nucleotide-binding site is juxtaposed to the RNase active site of PAN2 in the complex and may actually bind nucleosides of a poly(A) RNA rather than ATP, feeding the poly(A)-tail to the active site of the deadenylase and thus increasing the efficiency with which this distributive enzyme degrades oligo(A) RNAs.</text>
    </comment>
    <comment type="domain">
        <text evidence="10">The N-terminal zinc finger binds to poly(A) RNA.</text>
    </comment>
    <comment type="domain">
        <text evidence="10">The pseudokinase domain, the coiled-coil (CC), and C-terminal knob domain (CK) form a structural unit (PKC) that forms an extensive high-affinity interaction surface for PAN2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily. PAN3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCH6"/>
    <dbReference type="OrthoDB" id="204958at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031251">
        <property type="term" value="C:PAN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.3700:FF:000001">
        <property type="entry name" value="PAN2-PAN3 deadenylation complex subunit PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000520">
        <property type="entry name" value="PAN2-PAN3 deadenylation complex subunit PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.5160:FF:000007">
        <property type="entry name" value="PAN2-PAN3 deadenylation complex subunit PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03181">
        <property type="entry name" value="PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030844">
        <property type="entry name" value="PAN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041332">
        <property type="entry name" value="Pan3_CK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12272">
        <property type="entry name" value="DEADENYLATION COMPLEX SUBUNIT PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12272:SF11">
        <property type="entry name" value="PAN2-PAN3 DEADENYLATION COMPLEX SUBUNIT PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18101">
        <property type="entry name" value="Pan3_CK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25586">
        <property type="entry name" value="zf-CCCH_PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 10" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="213"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="213"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Knob domain" evidence="10">
        <location>
            <begin position="737"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="698"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="119"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="174"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="476"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="525"/>
            <end position="532"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="586"/>
            <end position="587"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4373234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="847" mass="92731" checksum="4D1FC9C82C9CCD1E" modified="2023-11-08" version="1">MSLGGPGRSQEPSAPYDFSPLMGSLSNAFAGTSHDIFGARLPTIQPASPNKGAPHASTPSSRPAFFPLPKGHPHAIAIAHPGSSTTDSASELTSSSSSRDSLPVAPARNGETTPQKQARNSRRNKTNKSNAKWHRGGSSDDTGSDRKPPTSQPSKPTQSASQSPRPASKQRQPSTTSTNSSAASQSRLMATTFGASSGDSRRGVVSPRPKGREAKNTFCRNVTIYGHCRYENTCPYIHDQTKLSQNENARKRFNVDSPAFTPLQASTNGSVTPSSRSAAISPKAANAAVFTPKSQRSTVSTPQLHTKEPALDWHAQDFQEFVPQAFESQMVDPNIGYDPFNTSTNIAAITGASHQATQINPYSQDHAATYYPNANAFQTTPAYHLYWPVGPQPTGLLAYQRTAHDFFIPDVIREDLQKKAEVARQTLPNTSLPAIEQFHSLFCLDTTPQKNTAPFGYASWIYKAISSKDGKTYALRRLENFRLTSETAIRSAQPWKRILNGSVVTTHEAFTTRAFGDSSLILVTDYHPNSKSLADEHFKPMQRFHGRQPTSSHVPEQVLWGYLVQLASALKAIHGSGLAARLITPSKILLSSKNRIRLNACSIMDIVQFENARSVAELQADDFVQLGRLILCIANSNATAHLQMQKSMDYITRSYTGRLKECIQWLLNPQNPSGTPSSPTTPGAMHKDIDTFLAGIADQFASVFDSELHAQDTLTNTLGRELESSRIVRLLVKLNMINERPELDASQTIPGNTTSNPSSAWAETGERYYLKLFRDYVFHQVDANGHPVTDLAHVLDCLNKLDAGTDEKIALISRDEQNILIVSYREVKRALESAFQDLVRAGRSSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCY3</accession>
    <name>A0A9W8YCY3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein PNG1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Protein png1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">png1_1</name>
        <name evidence="8" type="ORF">N0V83_002882</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transglutaminase-like superfamily. PNGase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCY3"/>
    <dbReference type="OrthoDB" id="409136at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000224">
        <property type="term" value="F:peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006516">
        <property type="term" value="P:glycoprotein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.620.30:FF:000004">
        <property type="entry name" value="Peptidase (PNG1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000011">
        <property type="entry name" value="peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.620.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050883">
        <property type="entry name" value="PNGase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12143">
        <property type="entry name" value="PEPTIDE N-GLYCANASE PNGASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12143:SF19">
        <property type="entry name" value="PEPTIDE-N(4)-(N-ACETYL-BETA-GLUCOSAMINYL)ASPARAGINE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Transglutaminase-like" evidence="7">
        <location>
            <begin position="229"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="398"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="400"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00071430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00071953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4374141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="443" mass="50413" checksum="4ECD47442BC21825" modified="2023-11-08" version="1">MDYSNGASRAPAANMDTDKLAAEMTEQFRRVLSTKRMSELTSRSSQSRSASPAPRDFGTPQPPSQNAPPTYESIKNIPIVPEAPHDARSLRFKNMLISLSNMPVRWENPGLLDEALRVVPLEQIYNEAEEESQILQAEAESLGAGKKAAWGYQDCVVRALLRWFKRSFFQWVNNPPCSRCYSPTVAVGITAPLPDEQARGAAQVEAYQCSHDGCKNYERFPRYNDAFVLLQTRKGRCGEWANCFSMLCRAVGSRVRWVWNAEDHVWTEVYSVHRKRWVHVDACEEAWDKPRLYTDGWGKKISYCIAFSADGAMDVTRRYVRDQTKHAAERNRAPEAVLLYIMNEIRAMRRANMTKQDKFRLQGEDLREGKELRGYVISSIAQEVSKLSAEDIIRAQVAPRRQDSEAPKALEGRTSGNSQWIRARGEGGRGQPNQQDPRNQHPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD18</accession>
    <name>A0A9W8YD18_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Chitin-binding type-4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_001877</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the polysaccharide monooxygenase AA13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD18"/>
    <dbReference type="OrthoDB" id="120613at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004302">
        <property type="entry name" value="Cellulose/chitin-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052282">
        <property type="entry name" value="Starch-active_LPMO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36575">
        <property type="entry name" value="BINDING PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G14430)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36575:SF2">
        <property type="entry name" value="CHITIN-BINDING TYPE-4 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03067">
        <property type="entry name" value="LPMO_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040974340" description="Chitin-binding type-4 domain-containing protein" evidence="7">
        <location>
            <begin position="18"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-4" evidence="8">
        <location>
            <begin position="18"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4374800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="209" mass="22517" checksum="1072EDC9EE6563B1" modified="2023-11-08" version="1">MLFTTAALVALIPLAASHGLITTPPSRPTGPAIIANCGAKVEQDIRLDNTSHVEGLPELAAKDSAYNAAKCNLWLCKGLQFADNPTANTQTWQAGQVVPIKIWLRIPHEGSANVSIVDTKSNKIVGDMLKVWAKGYAPGKKESDVPLDQTQFNVTVPEGLEDKCATAGDCVLQWWWYGTAAKQTYESCVDFKIAKKGMAEGSTARVFKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE24</accession>
    <name>A0A9W8YE24_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">methylcrotonoyl-CoA carboxylase</fullName>
            <ecNumber evidence="3">6.4.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">3-methylcrotonyl-CoA carboxylase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_001824</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3-methylbut-2-enoyl-CoA + hydrogencarbonate + ATP = 3-methyl-(2E)-glutaconyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13589"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57344"/>
            <dbReference type="ChEBI" id="CHEBI:57346"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AccD/PCCB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.4.1.4" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE24"/>
    <dbReference type="OrthoDB" id="439921at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905202">
        <property type="term" value="C:methylcrotonoyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004485">
        <property type="term" value="F:methylcrotonoyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000004">
        <property type="entry name" value="Methylcrotonoyl-CoA carboxylase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000007">
        <property type="entry name" value="Methylcrotonoyl-CoA carboxylase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045190">
        <property type="entry name" value="MCCB/AccD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22855">
        <property type="entry name" value="ACETYL, PROPIONYL, PYRUVATE, AND GLUTACONYL CARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22855:SF13">
        <property type="entry name" value="METHYLCROTONOYL-COA CARBOXYLASE BETA CHAIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="8">
        <location>
            <begin position="90"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="9">
        <location>
            <begin position="348"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00026116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4374748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="593" mass="63577" checksum="95B536B0E78D20E3" modified="2023-11-08" version="1">MVPKAPSREALTMLKHTIRPSIAIRSQVPRRLLSTASRASVLPQAQSNPSRQSSRKAATFTHSHHAEAVSIIPTTVETSSVDFKENKRQLDEATENLVNLHTKIAQGGPQKARDKHLQRGKMLVRDRITALIDPGTPFLELSQMAGYDMYPGEDVPAGGIVTGIGTVNGVQCMVVANDATVKGGTYYPVTVKKHLRAQTIAQENRLPCIYLVDSGGANLPNQADVFPDVNHFGRIFYNQARMSSMGIPQISVVMGPCTAGGAYVPSMSDENIIVENQGHIFLAGPPLVKAATGEVVSAEDLGGGKLHSEISGVTDYLAVDDAHALVLARRSVGNLNWHRNQSAPAQPTYQEPLYDPKELSGIAGTNLRRQIPIHEVIARIVDGSSFAEFKPLYGSTLVTGFAKIYGQPVGIVANNGILFSESSLKGAHFVQLCGKRHIPLIFLQNISGFMVGQDAEKGGIAKNGAKLVTAVSCVDVPKFTVVVGSSAGAGNYGMCGRAYSPRLLFTWPNAKTSVMGAEQLSSVMEAVGKQADPALKARIEHESEATFGSARLWDDGIIPPEHTRRVLGIGLQMACGGQNKGVEKESTWGVFRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE38</accession>
    <name>A0A9W8YE38_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acetyl-CoA synthetase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_002308</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE38"/>
    <dbReference type="OrthoDB" id="1706066at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050218">
        <property type="term" value="F:propionate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43347">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43347:SF3">
        <property type="entry name" value="ACYL-COA SYNTHETASE SHORT-CHAIN FAMILY MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="5">
        <location>
            <begin position="8"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="78"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="4">
        <location>
            <begin position="554"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4375222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="699" mass="77080" checksum="97298194EAA7FECA" modified="2023-11-08" version="1">MSKEHIQDQVYAASIADPEAFWIRQADNLYWHKRPSKALQKSTKHLKKSKVSHDHWSWFEDGEISTTYNCVDRHVKTGNGDNVAIYWDSPVTGQKERYTYKQLLQEVETLAGVLREEGVKKGDVVLIYMPMIPAALFGMLAIARLGAIHAVVFGGFSPAALAQRIEASRPVAIMTASCGIEGAKKPMEYKTMIEDAIQKSNLKPSKTIIWQRDQLRWDPVLRENGQRNWQRLVKSAKNRGLKADAVPVKSGDTLYIIYTSGTTGLPKGVVRSAGGHAVGLNFSMKYLFGIHGPGDVQFTASDIGWVVGHSYIVYAPLLVGATTVLFEGKPVGTPDASTFWRMIEDYKVTTMFTAPTALRAIRREDGDNEFFETRGKKGALKTLRALFLAGERSEPSIVQMYQELLSKHCAPGAMVIDNWWSSESGSPISGIALSASAGFDFSTSDRPKPLPIKPGSAGKAMPGFDVRIVDDQGNEVGRGEMGNIVLGIPLAPTAFTTLWWEDEERFYKGYLKRFNGKWVDTGDAGMIEDGYISIMSRADDVINVAAHRFSTGAIEQAITTHPSIAEAAVVGCPDALKGHLPFAFITLATHGHADPAVADDELFAEVQKLVREQIGAIASLGGMIQGKGMIPKTRSGKTLRRVLRELIENATHDEFDKEVNVPSTIEDREAVRVAREKVREYFQLKGRDLHKATEARAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEI9</accession>
    <name>A0A9W8YEI9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEI9"/>
    <dbReference type="OrthoDB" id="2107885at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005619">
        <property type="term" value="C:ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052786">
        <property type="entry name" value="Spore_wall_assembly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292:SF1">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN RRT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="243" mass="28139" checksum="C4094D59661CAE74" modified="2023-11-08" version="1">MTYLVKGIVFFIAHPFLYPLLKARLIPAILLSTFILVNLFVWTFLPQVAFLALFHKTGSAWVNGTFLVLGESAAVVAILFESFLVDESQVDIFDAVMIYRGCEDLVRQHRPVTEDSLNDPVRRLGKPTRSSVYAPFSFRQIAEFIILLPINFRGPFQHWRYFQLLGYDRKQRSAVIKRRRWQYTWYGTAYLFLQLVPPFSMFFLLTAGVSSALWAADLEKSRRDQEARLIQAPRYTDEPDATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEL3</accession>
    <name>A0A9W8YEL3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nitronate monooxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_002519</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEL3"/>
    <dbReference type="OrthoDB" id="10265891at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018580">
        <property type="term" value="F:nitronate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04730">
        <property type="entry name" value="NPD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004136">
        <property type="entry name" value="NMO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32332">
        <property type="entry name" value="2-NITROPROPANE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32332:SF31">
        <property type="entry name" value="2-NITROPROPANE DIOXYGENASE FAMILY, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G09850)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03060">
        <property type="entry name" value="NMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4373780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="340" mass="35922" checksum="97D23BD2867E9321" modified="2023-11-08" version="1">MLAGMDQVAGPALAAAVTNAGGFGTLGGARYTPQMLREMIAEMKGLLREKDAPFGVDLLIPQVGGGARRTNYDYTKGQLGELLDIIIESGAKLFVSAVGVPPQWAIDKLHKGGVLYGNMIGHPKHVHKACRAGADIIIAQGGEGGGHTGDIATSVLIPACADVCKQYTSPLTGKPVTLVAAGGINDGRSVAAALMLGASGVWIGTRFIVSKESKAPKEFKQQVIKADYDSWVKLLIWSGRPLRALSNPYLKDWEQNRQAEIKELTDKGIVPLEYELDRLHKEGKLTDEIEDAVALRPVGVVGGSVNKAGQSAAEIVEEIVQETLVALNSASTLVNPAAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEM8</accession>
    <name>A0A9W8YEM8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003503</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEM8"/>
    <dbReference type="OrthoDB" id="2129362at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="585"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="185"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="802" mass="90421" checksum="ED64C76DD966BC60" modified="2023-11-08" version="1">MSRVAYGFCTRDFMPAPAMEPPESALQPPIDTPTKPAKEAMTWQQQQDFGNATVSEENTPDQPHPYLRTLAIGLMQDKVLSPASNLVTFHPRFRGPCATFTFTSRSSLHSSSTPILAIDRLQAPKKRNSRRVKWQPYDQEAAEAPTTIATETASVLSAQRDNRPTSGPRRKARNKAATSGSILHNTQPAPNTPTTPEQTPDNDAQKTAKPTHRKPRPRRNRNKKDGNMDSLPKSAYIPPHLRKRGTATIAKVETEQDHHTPVVSPTASTKSKTEHQPPVQSSQTSAEEPQKSLPTPATTPKEPEKRGDNENNDWAGWDTPKAEPPPPARKAENPRWPRGPKPAPKKHVWPKASEMRYISGSDSDGGVSFKSDSNGDPHYDVKQLMDWNGDWLPPPEQWSARKGYTNRHFGQLMEKWMNGHGDKCTEMMDISSDTFRGRAEDNGDEDEYVLVDGVCKELVPRYWLSAQVEGKPLREFWKELPYRAPKALSDVDITEHPPWWDLYEDTSSCFISGVMVPEAKIDLGDVENHIDPYGMASAADKIQNKHNRSVNKHMRLMAKLNRVLPEPKVLLPPKEDRRIRPTANIYLRPVQPADVKGITDIYNYYVESTIAANEFDARTEAQMRVRINDITQEGLPYLVAVAKGNHSRGTVGYVSEKIVGYINLDDYCDQSSMYRYTFEMELFVHPGYTNKGIAKCLLDRLLEMANTGYNARGGYEYVNEFDYLKTGPSRVIKTILLNFHHEHGADAQWATDYLSAFKFVRAGRLSHVGFKKDQIVDLSIYQHHTTENINPSGIPTVPLERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF17</accession>
    <name>A0A9W8YF17_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleolar protein 16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NOP16</name>
        <name evidence="9" type="ORF">N0V83_001315</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the pre-66S ribosomal particle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NOP16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF17"/>
    <dbReference type="OrthoDB" id="285729at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019002">
        <property type="entry name" value="Ribosome_biogenesis_Nop16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13243">
        <property type="entry name" value="HSPC111 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13243:SF1">
        <property type="entry name" value="NUCLEOLAR PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09420">
        <property type="entry name" value="Nop16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="209"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="8"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4376035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="236" mass="26883" checksum="496BEFEC99CF0CDA" modified="2023-11-08" version="1">MGRELQKKKNKSSIPKKRQNGPSKKKLLQNPIIAKHWNQKETLSQNYRRLGLTSRLNHATGGTEKTIKLLGLNDGKSSRADTAANSTADALNIVSKAPERVQAEEVEVERDPETGAILRITGQQVKKDNPLNDPLNEIEDSDDEVEWNGFAMVPERREGENENPVIRQLEEAALNGARKAPRNQSQREQEWVERLVGKYGEDYDRMARDKKLNPMQQTPADIRKRVKKWRASQEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF84</accession>
    <name>A0A9W8YF84_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">rhamnogalacturonan endolyase</fullName>
            <ecNumber evidence="4">4.2.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ASD1</name>
        <name evidence="13" type="ORF">N0V83_002849</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-(1-&gt;4)-alpha-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end.</text>
            <dbReference type="EC" id="4.2.2.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the polysaccharide lyase 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.23" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF84"/>
    <dbReference type="OrthoDB" id="114708at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102210">
        <property type="term" value="F:rhamnogalacturonan endolyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10317">
        <property type="entry name" value="RGL4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10316">
        <property type="entry name" value="RGL4_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10320">
        <property type="entry name" value="RGL4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000102">
        <property type="entry name" value="Rhamnogalacturonate lyase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1120:FF:000017">
        <property type="entry name" value="Rhamnogalacturonate lyase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.10:FF:000020">
        <property type="entry name" value="Rhamnogalacturonate lyase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029413">
        <property type="entry name" value="RG-lyase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029411">
        <property type="entry name" value="RG-lyase_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016590">
        <property type="entry name" value="Rhamnogalacturonase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015364">
        <property type="entry name" value="RhgB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36574">
        <property type="entry name" value="RHAMNOGALACTURONATE LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36574:SF1">
        <property type="entry name" value="RHAMNOGALACTURONATE LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14683">
        <property type="entry name" value="CBM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14686">
        <property type="entry name" value="fn3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09284">
        <property type="entry name" value="RhgB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="12">
        <location>
            <begin position="19"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4374110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="834" mass="89264" checksum="EAEB5736CB713901" modified="2023-11-08" version="1">MSAGYFPKREKVGLEDLFKGLVTASHILHHNGIVDAYGHISVRSPDNAATFYMPCNMPPALVSSPDDLVEYHVESAEAVEKNAKPGYIERYIHSEIYKRFPAVNSVVHSHASDVLPYCVSGVPLKNSIHMAGFLGTNVPVWSASSSWSSSSKHDLLVRDETLGASLAASFKPATSAGFIYSKVRSALPSQLGGASSDPKLEPDHAVVLLQGHGFTTVAHGIEEAVYQAIYTKEAAKVQTTALTIQNAHTGYALEGKVDVEAGGKIKSGKAKTDGGELKYLSDRESHDAWESMSGTIARPWALWCREVEVNPLYKNECPTEVAFGVTKSGDNIVVDTGAANTLVFTVNAKSCDITSIKYRGEELQSSEKGSHISSGLGTATVTYTTVNSQYVKVTCTTSTLTHYLVAKSGDSNIYMATYTTAEPDIGELRFIARLLSSALPLEYPFGVVSTTVGSSSTVEGSDVFVVNGQTRSKFYSSERFIDDTTHCVYRDSDAIHACFLMNSYSYEASSGGPFHRDINTNNGGTYTSLTFYMNSGHVQTESFRQGLHGPYALAFSRSGVPTGGSSLDTTFFADLSITGYVAPSGRGTVTGTATGVDSSFQRVLHWYNSAAQYWAYADSSGKFTSPAMKPGTYTQVLYQGEFKVKAASVTVSAGKATTANIAADTESKTTIWKIGEWDGQPKGFRNADKQLRMHPTDSRMSSWGPLTYTVGSSSLDSVPMAIVKDNNPLTIKFSGTYTAAATLRVGTTLSFAGCRPSVVVNNYSPATPAAPTKIDSRGLTRGAYRGYGEVYNYAIPSGTLVSGSNTITISCASGSSGDGFLAPNVILDAIELYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG19</accession>
    <name>A0A9W8YG19_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG19"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="84"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="343" mass="39234" checksum="9DD1BF877E16DC61" modified="2023-11-08" version="1">MSTPTFSEEDLKKQIEGLKKLVYTHKKAVDLLLMPIVRDAIETSEQGYKAIISADTLPSIETIEKAGLRMGTCIKAGERFLTRHREHSDKLKNFQEEKLEAQQALDTKTALKRVRDLQTEAIKLCQEFSAEIWKLLEEVDKEKAEALKESNVVAKAYFESKFESEKGYMCFVETWYLPPVGFMHNIVRAHRYLMKVHMMRVAVEKVALLTGMIGDLRKDLAIRQIDALKKLSGDYLVKKARWQKVIDILKFHEQGIVDFERDTLKNLPSFTGDAEKHWVGEKEAELLIRCRVGFGFILGAFHSVEPNAAIKVKNMPDIKAHMESDNIGAFNDHVRKIIAELDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGI7</accession>
    <name>A0A9W8YGI7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peptide transporter PTR2-A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_000359</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGI7"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071916">
        <property type="term" value="F:dipeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000085">
        <property type="entry name" value="MFS peptide transporter Ptr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01022">
        <property type="entry name" value="PTR2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01023">
        <property type="entry name" value="PTR2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="425"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="454"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="496"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="532"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="564"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4377534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="629" mass="70103" checksum="C0E97E0901F65767" modified="2023-11-08" version="1">MPVGDDNVLQVTGLDHALVDRSGDPERKHPELHTTGPPTYLHSEGKAGLQDHYLHDADDFPTVEEIATLRRVPAQIPWKIYTIAFVELVERLSYYGTIAVYSNYIAKPLVTPTGAALDPDSDNAQPGALNMGKQVAFSLTTFNAFWVYCCPLLGAWIADTYLGRFKTIVYSVIVAEIGHIILVASSAPSVLAKPNSALAAFIIGLIVMGLGTGTFKPNISPLIAEQVPQERMRVETTAKGERVIVDPAVTTTRIYNWFYFFINIGALAGQLGMVYAERYVGFWLAYLIPTVMFIICLPVLWICKKFYVLKPPSGNVMGPALKLLFKALGNGFTLNPMKTWKNWNDGTMWEKVKPSHLGANKPKWYNFDDAWVDEVARGFGACSVFFWVPIFWLAYRQMDSNLTQMCSTMELHGVPNDVLSNLDPIAIIIIVPIMDSLVYPALRKRGIRFTPIKKITAGFILGSFAMVWAAVLQYYIYKTSGYYANPSDEYRSPINVWAVTGVYVLIATSEIFASVTTLEYAFTKAPKNMRSLVQSVQLFTTAFSAALAQAFTPLTNDPHLVWNYGAVAIISFVTGVAFHFAYRRMDQEEDKLNLLPTGHLGTEAQHNDIERRVSVATERRNSLPPTEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGJ5</accession>
    <name>A0A9W8YGJ5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">glucan 1,3-beta-glucosidase</fullName>
            <ecNumber evidence="14">3.2.1.58</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Exo-1,3-beta-glucanase D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">N0V83_000374</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Glucosidase involved in the degradation of cellulosic biomass. Active on lichenan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1-&gt;3)-beta-D-glucans, releasing alpha-glucose.</text>
            <dbReference type="EC" id="3.2.1.58"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.58" evidence="14"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGJ5"/>
    <dbReference type="OrthoDB" id="62120at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000033">
        <property type="entry name" value="Glucan 1,3-beta-glucosidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF34">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="18">
        <location>
            <begin position="498"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="149"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="354"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="149"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="198"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="259"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ4377549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="865" mass="99135" checksum="90816D32E61A7109" modified="2023-11-08" version="1">MPNDSDRDRDPSRRRPHGEERRRRRESHGELDTPERRRRRESRGENTVYDQEGRRRKGHRATDSQGDLLPKPPRRSRHQSDTEQESPRPRKARQSRRNSTTDGSGSRSRESGAPLSLDALAKLDKTNAKKAGGWGSYDYDEDYLKEVRKKEKNLEKDRVKHEREERKKEKREEEERRRAVESDAAALEEKARKRREERRKAAESKRAHTEDERERTRTVIESKTVVTEKEREKRRSAYTDDEREERRRRHEAKYTPSEAEERRERRRQEKQHRTEMKKRRVISGPLAEEGGIDDDEEYQYMMEKRGGGGSAPTVYTEEELAARKKKRKRIIIGIVSALILLAIIIPVAVIVSNKSKNSTPQTSSASSTSEPKTSNLDGLDRNSVPTADQGGILDPWSWWDTEDFNVTYTNETVGGLPIMGLNSTWDDSVQANPSVPKLSDSFDYGKTPIRGVNVGGWLNLEPFITPSFFENFGSKDGVVDEWTLLTKLGPTKAKDTMEEHYSSFITKQSFADIRAAGIDHVRFPFGYWIVQTYDGDPYISQISWRYLLRGIEYCRQNGLRVNLDLHGAPGSQNGWNHSGRQGVIGWLNGTDGDTNAQRTLDIHHKLSVFFAQKRYQNVVTMYGLVNEPRNVELDTAKVVAWSQKAIDQIRSDGIKGIIVFGDGFMGLDNWQGKLQGNDNLLLDVHQYVIFNVDQLSLKHRDKLNFACQAWTQQSERSMDTATGFGPTMCGEWSQADTDCTEYINNVATGTRWEGTFDTGNTSTSVLKPQCPEEGKCSCDPANADPSSYASDYKKWLYQFAIAQMDAFEAGWGWFYWTWETEKATQWSYRKGLAAGILPAKAYDRDWKCPDSPDDLDTFGGLEEYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH27</accession>
    <name>A0A9W8YH27_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Altered inheritance of mitochondria protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000213</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIM6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH27"/>
    <dbReference type="OrthoDB" id="4153866at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051236">
        <property type="entry name" value="HAT_RTT109-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571:SF5">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040999723" description="Altered inheritance of mitochondria protein 6" evidence="2">
        <location>
            <begin position="18"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="313" mass="34760" checksum="4F5598C071ACA249" modified="2023-11-08" version="1">MRPLAFIALATAPSAFALPDVSDALQNILKNTDKSDKYTYPTDFTREIVPKPLHSHNDYWRDVPFYSGLSYGAISTEADVWLINGTLHVGHELAALNKKRTLDSLYIQPILDTLHRQNPSSPFVPSGSTPNGVFDTSSGQTLYLFIDLKTPANVTWPAVVSALEPLRTGGWLSTYNGTAFNAKPITVIGTGNTQLADVLALEPRDMFYDAPLEKLNTTQYSNLTGNESPIASTNFVNVFGDVRKREFNSTQLDILQEQVAVAHEKGIMTRYWNQPAYPIGTRNAIWRILYDENVDLINVDDLKSAAEFWEASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YI33</accession>
    <name>A0A9W8YI33_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YI33"/>
    <dbReference type="OrthoDB" id="4664297at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2970">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="200" mass="21920" checksum="9E119E5F8B24049B" modified="2023-11-08" version="1">MANFWTSRTAIITGVVITLLGALYIYESDTPISFTMPKSSSSADNGVPGLEMKLFQLSRDPPSLRVTLKNTNPDTPYTILKWGTPLDSAALNTGVFSITDDNSGNEVEQVVLMINRKMPPPKEELITVAPGTEEAIEVVFDKPWMPEAKPAKYRVKAEGEVKGAWGKYGDDVTEEELNAYLDSPFSGKSFATGDIIMEVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CGU7</accession>
    <name>A0A9W9CGU7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_010082</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CGU7"/>
    <dbReference type="OrthoDB" id="271448at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="5">
        <location>
            <begin position="347"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="280"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="379"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="384"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="419"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="445"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4362965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="525" mass="58729" checksum="410D79C66CBF24C2" modified="2023-11-08" version="1">MDIPRGQAAMPLQEADFLDFIYNYFKAEYAPIRSFWSTATADPRQPSRWFNMGHELSPTTVRPANAQAQKDLESLDYFTRKWNLDAGFVRGLVIRFAKYEVHERLEKKLFECARLDLMVRKSLKEKHLIDALWPSPAPKNLPHELLAQGVQVRKWYGDMFNKYFRVLHSVDSFELRPEVDLRIKNSGTLMLIPFVDHLIFGAVDPIMPIVAKGAGYSNAKGWERRDKLGFGEPGRFEIRFEAPAEVTNTYTKSVVRQCQCMKCGTRRDVQVVVSVDPNPNAGAIASTSIPSGSRNANSSAPKRQSSKAAQRYAPDYVLPSEQEVAPPQPPRPQAQELEAPRALSPERKKQCPRCTFLNHPELTACEMCQADLPDATIAKPPSPAAVKKPSHSHSASVPPANTRTEALKEEARPAAPNRHSLSQSLFSIFPFSQQHQQEHHAKLPATQQADPSSTPVQSNQQRPTTPTANQTSRPSSKAGKQPKPEPPVPSHYDDTPRQVTPPPHSYLDVEPSTPLSRAPEMTMMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH83</accession>
    <name>A0A9W9CH83_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CoA-transferase family III</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009771</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CoA-transferase III family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH83"/>
    <dbReference type="OrthoDB" id="5863171at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="entry name" value="Crotonobetainyl-coa:carnitine coa-transferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050509">
        <property type="entry name" value="CoA-transferase_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48228:SF4">
        <property type="entry name" value="BLR3030 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48228">
        <property type="entry name" value="SUCCINYL-COA--D-CITRAMALATE COA-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="CoA-transferase family III (CaiB/BaiF)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="476" mass="51488" checksum="31BC33E4370E3987" modified="2023-11-08" version="1">MDRSSFTPFDSVAYIWKGLGLPDKALKSVYLPLDAECYPSSFKVGHIAQSSIALSALAAALLWSVRYQSPVANVTVPAEHAAIEFISERVYLLNSKSAPSSWGTIGGLHKTADGYVRMHDSFPNHRENALKILGLGNEASKGDVASKMLGRRSEDTESEAFKSGAVITALRSFDQWDALPQAKAITDFPILLKRIGGESPLPPRTPKSMTDNKCLRGIRVVEMSRVIAAPVAGKTLAAHGADVIWITSPTLPDLPGLDIDLSRGKRTVQLDIKKPDDKAKLQELLRTADVFLQSYRPGSLDAQGLSAEKLIEANPNLIVASLSAYGPEGPWSQYRGFDSLVQACSGINVADAERFGANEAARVLPCQAFDHGAGYLLATGIIAALYKRATEGGAYEVKVSLAGVMKYLRSLGQYTGQTGFERKDPKDLNDHSKYMETRESGFGELCSVRHAAHIAEVEVGWESMPKPLGSDAPVWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHT9</accession>
    <name>A0A9W9CHT9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHT9"/>
    <dbReference type="OrthoDB" id="18530at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40368">
        <property type="entry name" value="YALI0F14399P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40368:SF1">
        <property type="entry name" value="YALI0F14399P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040931027" evidence="1">
        <location>
            <begin position="19"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="227" mass="24771" checksum="1AF417893FCD19BA" modified="2023-11-08" version="1">MWLVVFAVQAFWLSLGTALSGQEPLKSLEEARGYNPGEPIPVSCLNRTIDTGEHITDAAGQLQYIPFPTCNETGRPLELFFGVEKEVNCTIDFVTDEFFHLLEFYIHSDAPLSCRIPSKPLPPSVLENEYRVSDQLTQEGALGTQSTLYTPLIIALAGTLQLSHLHVGNYLNLLVHAAPKSQSPGTVDAATAYSIASHTRNTKITIGDPLPLSFSCGGQRGGLRRVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI03</accession>
    <name>A0A9W9CI03_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010327</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI03"/>
    <dbReference type="OrthoDB" id="199574at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.990:FF:000006">
        <property type="entry name" value="Putative SAC3/GANP domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045107">
        <property type="entry name" value="SAC3/GANP/THP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005062">
        <property type="entry name" value="SAC3/GANP/THP3_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436">
        <property type="entry name" value="80 KDA MCM3-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436:SF4">
        <property type="entry name" value="LEUKOCYTE RECEPTOR CLUSTER MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03399">
        <property type="entry name" value="SAC3_GANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="2">
        <location>
            <begin position="348"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="529" mass="60315" checksum="6670B55B587BAC76" modified="2023-11-08" version="1">MSLPSTTLAWTRPQPPAYNAVTPRTTLQVLPANVSSRYRWDLPLPFDIYTALQPPTGTTSSKPKKVEWPPAVREYVRRAFDPANAIPGISSAEMQTKLKETISYYAERNAQDEVNWPTYPLPQELLQEERRKAALGMSAAPSNGFTNGFQHAYVNNLNDQTNPTIPQSKKRKSQDAEEMQAQAEASLPPWHKTNLASRMTFGDGHNDKRHKKSAASEAASKFEQLELEKRRQRFQLGQNGTKTTPPWGSPRKDEDDMPTGPLVGTNQTLEKSYFRLTAPPKPETVRPLHVLEKTLAMLTKKWKAEKNYNYICNQFKSLRQDLTVQHIKNAFTVKVYEIHARISLEKGDLGEYNQCQTQLKALYAQNLGGNPAEFKAYRILYFVYTCNKTDMNDMLAELTLADKNHEWIKHALDVRSSLALGNYHKFFRLYLEAQNMGGYLMDMFIERERLNALANISRGYPNVTLRFLTDELGFDNDETCREFLESHGAQNLIEAKDGNHFRVKIREGASLFENLRAAAFSKVDIKGQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI81</accession>
    <name>A0A9W9CI81_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Integral membrane protein-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_009836</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI81"/>
    <dbReference type="OrthoDB" id="10267969at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF80">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4363540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="23906" checksum="88295317F752032D" modified="2023-11-08" version="1">MKLPPIAVACLSALVIDSCSNVIAQRLKAWSESIPFVFDRVLFFQFVTMVVLTAPINYHWQNWLERAFPGWKTVKQTRFADAGEEEKGIMLKEDGEDKRVVEEEIRVRNWWNIFRKWFTDCITMGALLNQSLFLILMGIMKGKTVALIVQDFRNELFGLIFDSYKVWPIANFFSTTYCPVERRIIFLSFCGLLWNIYLSLVAARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIF4</accession>
    <name>A0A9W9CIF4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Iqgap- protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">IQG1</name>
        <name evidence="4" type="ORF">N0V83_009538</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="775356at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21206">
        <property type="entry name" value="CH_IQGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05127">
        <property type="entry name" value="RasGAP_IQGAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000593">
        <property type="entry name" value="RasGAP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14149:SF14">
        <property type="entry name" value="CALPONIN-HOMOLOGY (CH) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14149">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN WITH IQ MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03836">
        <property type="entry name" value="RasGAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143885">
        <property type="entry name" value="RGC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="3">
        <location>
            <begin position="265"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="2">
        <location>
            <begin position="1030"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1439"/>
            <end position="1459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1449"/>
            <end position="1459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1690" mass="193931" checksum="721315096532EC48" modified="2023-11-08" version="1">MSYSSYSPRTRLGHLATSPSPLRQTSTASNGSSNYSNGTNQYPPSRSSTRSSDASNGLGSVGHRRGVSEATALSQPRPEDYFGSEETDPSTTYTSMRRALRPLQQVPQSTPVPDKPVRPIIESPTHSHVRSQSVENIRYSPYDGSVSPPAKPRPLSLAISRADSVRAPTRDHSNRPLSTHLEQPELQSFQKSSTGHLRTLSKFAESASDEGFAIKSPEHQVVGLQGRRRLQRTDDSQPGKKTASSWASRNWMDQQRQFLQAYEYLCHIGEAKEWMEDIIETVLPPVVELEEALRNGVTLAEIVQSIKGKPLRIFRDPKLQYRHSNNYAHFFAFLSEVELPDLFRFELIDLYEKKNIPKVIYCIHALSWLLFRKGIVDFRIGNLVGKLQFEDHELEATQKGLDKSGVSMPNFSGMQANFAVEPEPEPVESEEDRIDRELGENEDTILDLQAQMRGALVRIRLGNLMQDLWDHEHLVVDLQARIRGDWARQIAYYRMDMRRFAVTLQSAARGYLVRSRLRGEEQYWQDKEVQVLKVQNLFRGRKARAEVQYTKSKIHRHDHGIRQFQAAIRGALERLRVGDRYEQTRDTEPAVLHVQSMIRGALLRQQVDRQWGELKDSEPQIMQLQALSRAVLLRKSLRADQESLRQNESIVTMLQAAARGLIARSKNADVQKKLVSSKVNWSDLQSQVRGNSIRSSIRQQQEALQAEVPNITDLQAVARGGKVRSGVADTLAQLEEQQSVVLSLQSYIRGFLSRQRHFSDLKALQAQTSTILDLQSACRGFVQRQRTYEILCELNAQEPEIIELQSVARGVLLRMQIGVQLGQVEEHEDTIIELQALARGMLIRQKFVEKKQFYKENMEKVIKVQSFIRGRQQGEAYKSLTSGTNPPVSTVKNFVHLLNDNDIDFDEEIEFERLRKTVVQHVRQNELAEQYVDQLDIKIALLVKNKITLDEVVKHQKHFGGNVGTLLSSKDISSKDPFDLKALNKNSRRKLEQYQELFFTLQTQPQYMARLFRRVREQTLAEKDTKRIEQLTMSLFGLAQKRREEYWLLKLLARSLKEEVEGCATLQEFLRGNFFWAKLFSNYVRSPRDRKFLKEVLGPVIRENIIENEHLDLESDPMQIYRSAINNEELRTGQRSHRDPNVSRDVALKDPETRGTFIHNLQDLRDVADQYFSLFEEVLHRMPYGVRYLAQQMFEELRQRFPYEAQEQLLQLAGHWVWKAYIQPALIQPQHWGVVDRGLSPVMNRNLGAVGKVLGQIASGKLFGGEDLYLQPLNSYITEAIDRLQDIWSNLINVRDAEAQFEIDEFNDLFARTKPTLYIKLADVFAIHNLVIDDLPTLCPAHDDPLREVIRELGSAKNNENELLHVSSTEITLTLNPKYHEQEDPDAQIKSLFMETKRYVLYIIRVQTGANLTEIMTKPITPDDEDRWDALVREELAAQQKDRSRKQSAVRSSAASTYTTDMGSNSVLDLDYPSLKQYCLENMVQLQRAGRISHHNNYQDLLNAIAVDIRTKHRRRIQRAKELEGVRTTLGALDEKAHFLESQLKSYNDYIEQAMVTLQSKKGKKKFILPFTRQYNHQRELQRAGREYRFGSFKYSARNLAEKGILISWSGYPERQWDRLDITISSNSTGTFEIQGSQGSMMIPGAFAEVPLDGLLQAQFDNHQFMNLFGEGRDGSGGGRLGLMLICFCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIK3</accession>
    <name>A0A9W9CIK3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycolipid transfer protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009047</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIK3"/>
    <dbReference type="OrthoDB" id="205255at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902387">
        <property type="term" value="F:ceramide 1-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902388">
        <property type="term" value="F:ceramide 1-phosphate transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3520.10:FF:000001">
        <property type="entry name" value="Pleckstrin domain-containing family A member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3520.10">
        <property type="entry name" value="Glycolipid transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036497">
        <property type="entry name" value="GLTP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014830">
        <property type="entry name" value="Glycolipid_transfer_prot_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10219">
        <property type="entry name" value="GLYCOLIPID TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10219:SF25">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY A MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08718">
        <property type="entry name" value="GLTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110004">
        <property type="entry name" value="Glycolipid transfer protein, GLTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Glycolipid transfer protein" evidence="2">
        <location>
            <begin position="32"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="205" mass="22674" checksum="3D80921B65763FA1" modified="2023-11-08" version="1">MATTYPPGGTFFDTVKKSFVDVPVDASKDSAISTTEFLEAAESLTTLFDVLGSAAFKPVKNDMLGNVKKIRDRQLAAPALSETLQDLVLNELKEKKHTATEGLVWLNRGLDFTAQALRHNTSNSSKELSDSFREAYGKTLKPHHSFVVKPLFSAAMSATPYRKDFYKKLGDDDSKVQAELEKWLAALEKAVGVLNGFLARKEAKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIM6</accession>
    <name>A0A9W9CIM6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_009615</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIM6"/>
    <dbReference type="OrthoDB" id="10262656at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17330">
        <property type="entry name" value="MFS_SLC46_TetA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF8">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G03730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="437"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="468"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="520"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="468"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="531"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="554"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="590"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4364160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="602" mass="65551" checksum="03DBC21088174A34" modified="2023-11-08" version="1">MPELMESFGVPENDIARWAGIASSTFSICQAATGIIWGSLSDRYGRKPIILLGLFNTMWSMLLWGFSVNLPMALAARALQGLTNGNVGILRTTVAELCPWKELQPRAFSIMPLVYTVGAIFGPTLGGALSNPLRVDPRKPRGSGFLEHFPYILPNLVAAIFFTVGIVVGWLFLRETLESKRHEQDLGLRTGAKITAFVRRTLNMPKKHKGLPREREPLLGQRKAFDGEATARLGVASEIVKEDSPKIRDVLTYQTTLNLAVYTLLALYTLAFDQLLPVFMHHPTQSLDDPNVRLPLKFAGGFGIDSRRIGAIFTFFAVSSTLCQFLLFPPLARHLGVLRCLRIAFCIFPFVFFVTPFISLIPDPTTRQIVMVALLMVRGVGGTFAFPTSTIMLTNSASSLRVLGTLNGLATSLSAVGRATGPTIGGGLFTWGVKRGYVIIPFWTMSAISLLAVIPTFWLVEGKGFGDDEDSDEESLTLAEGEEEEQATRPRPIGVEEEASLSESEFGDIGAPPNLLSYTSRRSSAAMTSDDGGDDSDDEAYSGRRRSSNALGRTRSHSQGRRARVVRRRTSVPIGMGQGFRRYSSNLGSTGVGAPGTSWGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJJ9</accession>
    <name>A0A9W9CJJ9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008447</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJJ9"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112:SF10">
        <property type="entry name" value="TOL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="84"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1142" mass="129371" checksum="70A4C968706E0805" modified="2023-11-08" version="1">MSLFRKANFTDAKLPLDWSAEEVNTDIEKHDFRMMERRWKRKAGSIWGPSLINDFHVAQWKFHAPVISAAVEHHDFGRRTLPFIHKNTALSKGAYGIVYKYEIHRAHFKDPLSMAESPSYVFAVKELNKIDGKVARHWGAEVRALARMNKLKQKHIVRFITAYRRGKPDDLEHYVMFEWADGGNLSTLWERDRQPELSVARVKWVIEQLRGLAQALSAAHYLREDDGQYHGGSYRHGDLKPANILWFREGDEFGTLKIGDWGEAKEHDAVTSLRHSTTAQFATRRYEPPEVVTGLQLTLSDEAKDVRSRLYDIWSIGCITLEFIIWILYGEDGRKKFDKNNRGHYGVSDMFYEVNSEGIASVHGAVKYWIDQMANDPRCRPQKTALGDLLEVVRDGLLVVKVPRDGGPIRSYVPTPQVFSNPDLNLSGKVSTITTTYGQTILQTDAPSDVPSIKIDQAGPMISLPTPEHNSQAAERFPATELAEQLSRIINANPAESYWCNGSPARPAPVYPTKSFSSSLSIPRKSNSEGPMIPVLDKVDYGNPKLDPDDWNFILDNTFAANLLSNLLGSSIVPPHPSIPDSICSRCQEFHDKMWSPIVRVSYDTQQLMHSALGQTCNLCCLLWQTCKEYSRTTFSTVEFLRSDSTLTLRGVRQPVLTLFRRNKFKGRASDIQIGLTELPNAGDDLHLGVLRGWLDDCDNDGDNIIRLKETKNQDCGDWVALSYRWGPLPHFSTTQQNLKEHIAGMEWAKLPLTFKDAIRVTRALGRQYLWIDSICIIQGEDGDFSEESKRMEDVYSGAYCVLAASCATGQKSGFLLPRPKKRKYVALGSPNEDDDDGIYVCERIENFKDHVSKGALSQRGWVLQEHALARRTIFFTEHQTYWECGDGVRCETMAKMTNDYAALLGDPEFPKILNPAKHGEKITRFQELYQQYSRLDLSKDYDRPTAINGLQQRLLRTMGVRGGYGMFDDCENKGLLRRSLLWHRGSDTESLKAIVFPPDRETVPSWSWMGVSGGIDYFPLAFFRFDWQDIVSPWAEADSAKASVGIALSAEAHEFDKLEDELSEQEIIFDNPADCEQYETMAVVLGIEKGQRALEDKKHYILLVTPRDPSDSLRPTVCKRIGAGYLPGKCFTGESRICSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK02</accession>
    <name>A0A9W9CK02_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK02"/>
    <dbReference type="OrthoDB" id="3693282at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="344" mass="38214" checksum="0427A112741DD23C" modified="2023-11-08" version="1">MAPKRAAPVPVRGNKSILSFFSALSTTSVNESQPSPGKKLEQEELSMDSLGRTGSKIDTSVSSDEDNAVADVFDASEDSQVDGIRTTRVRRFNGSFRVVVNEYGQEPASVVVLARSSSHAPKSWHQGREFLSEGMPIFAEKLFRTFEEDNKGIENLAGVFLAMYDIHTRYVPTGEHCEAHKVQDPSMKSYLSRAFKFTDAIDKELNSIAIASPDRARYLLSVGLDGFACVADLFLAWLKRTPPFILVIRETELCYGMKEEFFPMSSQGLYYGFFDSQDLEKSIESGLSTDKAIENLLEAWAKCQAWKDDISTKNNGRTIGRNGDHKAIPTRAKRGPSRGPTMPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKD9</accession>
    <name>A0A9W9CKD9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006561</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKD9"/>
    <dbReference type="OrthoDB" id="5416172at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="149" mass="15895" checksum="5F851EFD51C2E1F7" modified="2023-11-08" version="1">MAPDPHQGGRLEDMAAEGTSIPNDAGKQNLIPSVPRPDQINPDPSLAHANPAHGADNAFDIPRSVNDRGQTGEIFTGTGDSMSSNLEKKNLDDANFDPRAKGQDRYAKHARQDNPLDSLKHGAGSHDVEAAPGEEELGQDELLDKRGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKQ4</accession>
    <name>A0A9W9CKQ4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006977</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKQ4"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4367395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="66" mass="7531" checksum="C8CC62B5D3CE1005" modified="2023-11-08" version="1">MFYARPEPPSPWDLVMMNLGSLRRKAAGGYVDARTRDLGKEPETSIEEKNVKTADEVVEKDFVKEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKR2</accession>
    <name>A0A9W9CKR2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_006424</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKR2"/>
    <dbReference type="OrthoDB" id="415706at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001425">
        <property type="entry name" value="Dynamin GTPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF215">
        <property type="entry name" value="DYNAMIN GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="5">
        <location>
            <begin position="33"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="4">
        <location>
            <begin position="705"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="471"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="473"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="490"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4368069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="793" mass="88527" checksum="E1B62BC43C38992E" modified="2023-11-08" version="1">MVNGALTPESDLLANPTLLRKIDQLREKNVGQHVPLPQLVVVGDQSTGKSSLLESLTGIPFPRNLELCTRYATQITSRRDPESRVDVSIIAGPNASDEHKKHVAKFHPTISSTENLRAQLQEIMKEANVQMGIRTDLVSHEGTVFSEDILRIEMCGPNEDYLTVIDVPGIFRNHVKGLTTPADIQLVQKMVKDYIKHSRTIILAVLPSNVDIATQEVLTIAENYDTTGERTLGVLTKPDLVTEQSAKVTVCNLVLGKRRPLKLGYYIVRSRGADDDDTFNRNQAENMFLEAPWNTLPKERLGATALKARLGELLGQITRKEFPKLRKEVTKKLSECQTTLQRLGPARQTEREQRVFLSSVARRFQELVDAALNAQYSSHALFKEVEPRLITYVTNITEDFSNEFERKAHLRNFETTNRKLGEKSANIVPEAANIFNGFRVSNNAGGGIFGKLASPARGQVVFGQVSGIPPTGLSATSGQPAATIVSNDAPADDGKDDSDDDSDDDSDDDSNESAEYTVLENIISKHCVIEEPKGDIMGWAETIYKESRGSDLGTFNGAILASAFKEQSSKWPAMAKKYVSQVIVAIHRFMVTAIDSICADKQVREEIWVAILDDVLKRYKAAIDQAEFLVSLERDKKPYTMNHYFNDNLQIARGKRKADMLEGRARKELKMGDGDKAPQFSRNLIVDLEDVRSATSNMSNVQQVKEEIHDILSSYYKVARKRFVDNVYQQAVDHCLLTGPTGPLNVFTQEWVIELDAERLETIVGETLATKQQRLSLQKKEQDLLAALHILKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL87</accession>
    <name>A0A9W9CL87_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Arginine N-methyltransferase 2</fullName>
            <ecNumber evidence="8">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RMT2_2</name>
        <name evidence="11" type="ORF">N0V83_005990</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">S-adenosyl-L-methionine-dependent protein-arginine N-methyltransferase that methylates the delta-nitrogen atom of arginine residues to form N5-methylarginine (type IV) in target proteins. Monomethylates ribosomal protein L12.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class I-like SAM-binding methyltransferase superfamily. RMT2 methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL87"/>
    <dbReference type="OrthoDB" id="19014at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019702">
        <property type="term" value="F:protein arginine N5-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000135">
        <property type="entry name" value="Arginine N-methyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017408">
        <property type="entry name" value="Arginine_N-MeTrfase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051038">
        <property type="entry name" value="RMT2/GAMT_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026480">
        <property type="entry name" value="RMT2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32379">
        <property type="entry name" value="GUANIDINOACETATE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32379:SF1">
        <property type="entry name" value="GUANIDINOACETATE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038148">
        <property type="entry name" value="Arginine_N-mtfrase-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51559">
        <property type="entry name" value="SAM_RMT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RMT2" evidence="10">
        <location>
            <begin position="176"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="132"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="133"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR038148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4368908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="398" mass="44461" checksum="AAC81FC558345983" modified="2023-11-08" version="1">MAEQATEEDILLAKRIISAAERHDVPALKVLLKEGSANVQDPTASVATSPLHAAIIACGKAEEGKEAAVDAVQTVELLLQNGAIWNDLNKEDETPGCIALRLGQKKIYDMMVEAGVRAEILFAKMAALGLGGDADDEEEEEDEISEEQPAAKKQKVSEEDAKAIQEAVEEQVLDDVSLDNRAYLKSQLRYKPGILLDESDNAVMMDWETQIMQRHAETLIPKSGLRTMNVGHGMGIVDTAILTHDPAEHHIIEAHPQVHARLREQGWYDKPNVTIHEGRWQDILPKLVEQGVVLDAIYYDTFAEDYAALKEFFAEYVIQLLAKDGKFGWYNGLGADRQICYDVYTKVAEIDLYEAGFDTAWENIDVPSGLHGSKQWEGTRRPYWTIDVYRLPVNTFVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLG2</accession>
    <name>A0A9W9CLG2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005902</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLG2"/>
    <dbReference type="OrthoDB" id="5305306at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="342" mass="38515" checksum="73D71384808A2BE8" modified="2023-11-08" version="1">MADIVETDGGVSNGGKSIHHPRDMRENQATSSTSDGSGDTLHEETAHDASSNGEGATSLSSPLSPVLGLDNLTIDEDAARFYKGGTQRYTSPFHRGAKTSHDSTPYGFSRQDIARKGTNSRSVAPHNPRNGAAGYRNSRTWMSRDQQAQQEFMVVRNAMRRLFKYSEVAQWKLPDYIAHREAMVASQAARLAEQVKVKEEARTTTPISQEVQHNLRKWGLQGNFDECGNFGRVLGERTIWCTDWMNGKDEIAPWPSMAEMKWEGDDRAKTGVGRFLPFPREEGPPSLLWSQLPCVDQYLMDQVARIPTMEDIYLPVDDQIEPDHEYLWSKDLEKEMYALLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLG7</accession>
    <name>A0A9W9CLG7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ferroxidase</fullName>
            <ecNumber evidence="3">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">YFH1</name>
        <name evidence="14" type="ORF">N0V83_005917</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>4 Fe(2+) + O2 + 4 H(+) = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the frataxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLG7"/>
    <dbReference type="OrthoDB" id="1897642at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034986">
        <property type="term" value="F:iron chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.920.10:FF:000004">
        <property type="entry name" value="Mitochondrial chaperone Frataxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.920.10">
        <property type="entry name" value="Frataxin/CyaY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017789">
        <property type="entry name" value="Frataxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002908">
        <property type="entry name" value="Frataxin/CyaY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036524">
        <property type="entry name" value="Frataxin/CyaY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020895">
        <property type="entry name" value="Frataxin_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03421">
        <property type="entry name" value="FeS_CyaY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03422">
        <property type="entry name" value="mito_frataxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16821">
        <property type="entry name" value="FRATAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16821:SF2">
        <property type="entry name" value="FRATAXIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01491">
        <property type="entry name" value="Frataxin_Cyay"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01219">
        <property type="entry name" value="Frataxin_Cyay"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55387">
        <property type="entry name" value="Frataxin/Nqo15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01344">
        <property type="entry name" value="FRATAXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50810">
        <property type="entry name" value="FRATAXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0409">Iron storage</keyword>
    <keyword evidence="6" id="KW-0410">Iron transport</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4368835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="198" mass="22138" checksum="5E4FA9D78A20AC67" modified="2023-11-08" version="1">MRPASRIGSALLRRTIRTPLRAPLPANCIIARASVISNASAVRAYHPSMRLRSIMPDSENPPPREAESTDEPTVPTDIPTSEFHERADQYLNELVQRLEEAQENDPNIEVDYSAGVLEVTSKENTFVLNKQPPNKQIWLSSPISGPKRFDWVVSQEGMDFKEGGGQGDWVYLRDGTSLTEILRKELGVDVSVDDQVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLU3</accession>
    <name>A0A9W9CLU3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLU3"/>
    <dbReference type="OrthoDB" id="8033832at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="303"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="429" mass="46114" checksum="998029D79869643A" modified="2023-11-08" version="1">MSDSSSDQNQNQRRVPEEKGPFGKARKRGTSRAKTPTTKSAADEAKLENLRAIDDIFGRFKVDEDKKRSVSARSVAALPPSASAPNLIDAGGLATSFQASGAPPNGAIKEPKEVVLYGYGSDAQWAAISHYEKVSGGLIYEEYDREPYNPKFGYSFTSQRSIHYKTLTKSALRKVNEFVGGDHWIKVTFDSAEAAERACHYSPKSIQGWTVYAEPYRGTGPTVGDKAIRAEAGALSQTASPNTVSSGTVGARGASQSSTTVSSATATASIPASQSFLPRSATESSLTRAASGAFPSTNDDLYAAPAPQTQSQTQLQHQDSANTTATALNPRSRRATLRLKGANVKPAVFLPQEKAFLPSPPRWQQTFGSLPIIGWVIGSGQGIIGDQVPRKEDGTFDEQSASLYWRVWYMVDSCLGSDFCGVKDAEYDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMH0</accession>
    <name>A0A9W9CMH0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_005676</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMH0"/>
    <dbReference type="OrthoDB" id="5296287at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF163">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="275"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="52"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4369912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="336" mass="36620" checksum="3170871FC4FBA24D" modified="2023-11-08" version="1">MVLTDEEQPLLRILSIPGDSADGVKHDEVILDFEPLDPENPRNWTSSFKRFIVLLLALMAFTVTFTCVGVVPVASKIVEDLDGKHAPSSTSALLVTIWELGEAAGPLFIAPLSEVFGRYPVMNICNLGFITATILAVSSQNSKLFIVARMLTGVTVASNVLNPAIIGDMFESEERGSAMSLVMLAPLIGGAIGPAISGTIAQTMGWREILIIAIGLAVVCEVLFLTCFRETYKLAILRRRIARMQQESGESRHPSKTKTKHEHVLKLWHSMTRPFAVLFGSSVLMLVSLFASVAFSFFYVISISLPNILKDVYGFTPAQIGSAFMSFSKQLSCDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMI9</accession>
    <name>A0A9W9CMI9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_005003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMI9"/>
    <dbReference type="OrthoDB" id="10256055at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41677:SF1">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41677">
        <property type="entry name" value="YALI0B19030P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4370483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="385" mass="43103" checksum="825CCBDA2E1B5FE7" modified="2023-11-08" version="1">MATIANQPNSAPLRQTPMSTLLDSAATHEGRHANIPAFDPEKHLTFNEAPEFLTMKDIGLAEDIGISPVAVTQPFQLFSPEAIQIMRSEISKSEVTKNHTFSSNIAAAQLRGYARDHAPFTYAAWTHPKTCAIVSKLAGVDLIPWGNYEIAHINLSLKSEEQVKAELNAIQQRKRIHADEGIDMGGSENDKPIVGWHTDSYPFVCVLMMSDCTDMVGGETALRTANGEVIRVRGPTEGCAVVLQGRYITHQALRALGAKERITAVTSWRPRSPFVKDDTELRTVRPVSDLNELYYDFADYRLEIMEQRCRRAREMMRARRQDGGKFDMTGHKFFLAESSAFMQRTNHELVEEEKVRKGSIETVDIPDVVVDNGQATPTAKRFRVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP14</accession>
    <name>A0A9W9CP14_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Nitrogen permease regulator 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4 5">Required for meiotic nuclear division protein 11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">npr3</name>
        <name evidence="8" type="ORF">N0V83_003162</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 5">Mediates inactivation of the TORC1 complex in response to amino acid starvation. Required for meiotic nuclear division.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Vacuole membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the NPR3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP14"/>
    <dbReference type="OrthoDB" id="18648at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056603">
        <property type="entry name" value="HTH_NPRL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005365">
        <property type="entry name" value="Npr3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13153">
        <property type="entry name" value="CGTHBA PROTEIN -14 GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13153:SF5">
        <property type="entry name" value="GATOR COMPLEX PROTEIN NPRL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24064">
        <property type="entry name" value="HTH_NPRL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03666">
        <property type="entry name" value="NPR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0469">Meiosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GATOR1 complex protein NPRL3 C-terminal HTH" evidence="7">
        <location>
            <begin position="815"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="54"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="217"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="253"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="650"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="66"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="255"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="271"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="696"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="737"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU368069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4372871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="867" mass="96333" checksum="51E9CCBE41F66610" modified="2023-11-08" version="1">MAVPLPPASNLHAILLVIESRSLGPRLVFHYPPLSPSAAALAAAKDPAWFRNQTSTASVDSRSSDSDWDSSTDSDDENDIEIGSRTSGGRGSGRTVTGSNYRERDRSKLGTGVWGKQETIDEEDADDGNGDGELGGRRQKGGDHDWETVLGFKTDALEKMLSPKREYNKRRFELGVESIVFVGAPMFIRDDGLWKKLKRKKKKRSEKEKLEDGDLVKNLTIADEDEDANSPDDPVKPVPEPFIYPEGFEPGYGHGSMSSAPSGAPSEAGSDTRSNSTSQDNNPDMTMFNVVFVLNPPVLEYQQRVKEMYDNVTRKYAKALKYEEARFQYVWKESKRIIDIKQRAKDNNESLTVTWRKIVSMSPLAKSIAIMFDAISHDKIAHIHFDATFNTSFQIPQADSTPYLPNALEPQMPGLWLTTSNVVLEDDESPMTQHAALLLLEDTETLIKDLGGESTGNAAAIAFYIRNIIPTKSLLKISKRHNVLAHDMEYIASHLVYWRRARLIAPLSPRDTYIVSPNADMTVLPAAITVYAQRFPTLPTLPKVLSMLSGTPRPYRTFIPTAEHREAYMEILAWLMRGGWVTQLRTFAWVRVTPEIKAQVAADIEREDRIKKAEEARKELMDENESLADTLLSDKRNSLLSLSSARSLTPLRRTRRDRDASADGEEDMESSRILSPRLTAATTSNHRASPARAGSDTGSTSSQLTTIALNPSQRAESPSHLGVKDAKPHRPSPLHLNPTSPSRGSGVSDTSPAEAVPTPNPKDFTHSTVLSPQKASTLETLWLDKIAETFEDTNIISTNPSPPKNVDGGMGITGKELRDSWSLLLRHLDGKHAIEDVSAREGIKRKRVALLFNAVRERGWLVVTRHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPH2</accession>
    <name>A0A9W9CPH2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">40S ribosomal protein S22</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RPS22</name>
        <name evidence="5" type="ORF">N0V83_003645</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPH2"/>
    <dbReference type="OrthoDB" id="10250260at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000001">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047863">
        <property type="entry name" value="Ribosomal_uS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003115">
        <property type="entry name" value="PRK04034.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4373350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="130" mass="14854" checksum="EA0A2937942B9330" modified="2023-11-08" version="1">MVKTSVLNDCLNAINNAEKAGKRQVLIRPSSKVIVKFLTVMQRHGYIGEFEEVDDHRNGKIVIQLNGRINKTGVISPRYNVQLRDMEKWVVKLLPSRQFGYIVLTTSAGIMDHEEARRKHVAGKILGFFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPM1</accession>
    <name>A0A9W9CPM1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003647</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the choline/ethanolamine kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPM1"/>
    <dbReference type="OrthoDB" id="10267235at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004103">
        <property type="term" value="F:choline kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004305">
        <property type="term" value="F:ethanolamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603">
        <property type="entry name" value="CHOLINE/ETHANOALAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603:SF93">
        <property type="entry name" value="RE24176P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01633">
        <property type="entry name" value="Choline_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="177"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="255" mass="28790" checksum="C63477001C5D7263" modified="2023-11-08" version="1">MQTEWTYNYHDAEASWRCKTKYYPTVEEQHRFIRAYLLHNPTYKAQGGYTSNPPTPHLGPLPSSASTTALAATAAPSSISAFMLDSRAPPGEKHTSYQDQETQAERQTEEECRRLMAETKLWRLANSAMWVAWGIVQAHIPGMPDFEAEKKKADAGKTAEAEELESATAELRAVAEAEEKKGSVSQEKADKKDDAKVPSDEETDLFKSQDEEEFDYLAYANDRAMFLWGDAIRMGVVKAEELPEELQKKVRVVEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPS7</accession>
    <name>A0A9W9CPS7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="17">DNA repair protein rad2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">RAD2</name>
        <name evidence="17" type="ORF">N0V83_002722</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Single-stranded DNA endonuclease involved in excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Essential for the incision step of excision-repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the XPG/RAD2 endonuclease family. GEN subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the XPG/RAD2 endonuclease family. XPG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="31113at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048256">
        <property type="term" value="F:flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09904">
        <property type="entry name" value="H3TH_XPG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09868">
        <property type="entry name" value="PIN_XPG_RAD2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000025">
        <property type="entry name" value="DNA repair protein RAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000030">
        <property type="entry name" value="Flap endonuclease GEN-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000061">
        <property type="entry name" value="Single-stranded DNA endonuclease (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001044">
        <property type="entry name" value="XPG/Rad2_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-G CELLS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171:SF7">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00066">
        <property type="entry name" value="XRODRMPGMNTG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00842">
        <property type="entry name" value="XPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="16">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="15">
        <location>
            <begin position="944"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="514"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="663"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1174"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="667"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="694"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="716"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="751"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="777"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="867"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1194"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1225"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1256"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="1277"/>
            <end position="1291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4373983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1291" mass="144750" checksum="115FA8EE54F75F89" modified="2023-11-08" version="1">MGVTGLWTVLAPCARPIKIETLNKKRLAVDASIWIYQFLKAVRDKEGNALRNSHIVGFFRRVCKLLFIGIMPVFVFDGGAPALKRQTISNRKSRREGRREDAVRTAGKLLAIQMQRAAEEEEKKRKEAARHPRAQQQEEEIPENLVYAEEILQTQQERTQNRTFKRKDQYHLPDLGMSMNEMGGASDPRVMSLEELEEYASLFDRGEDINVYDFSKIDFDGHFFQSLPAADRYNILNAARLRSRLRMGHSKEQLDAMFPDRMAFSKFQIERVRERNDLTQRLMNLNGMNDDAFGAGSSSRVSGERDREYVLVKNDGVEGGWALGVVTNKDEGKLHKPIDVDLPQRVAEEDEEWEDEDDFEDVPIEGLNRLPKSKPGLSAQEKEGREFIANELATRRRRLYNSRKDKPVQRLPQRASPVNQDPDSLFLAEDNEDAEWENVPATNVDALFDEAGDDVEDQQLQQAIALSMQQDGQDPVEESEDDPVREIFQQKRVEESNPFCGTKASGLAIARLANQRSSKLAPKGPFEGSDSEDDMDLQAALAESRKSKRPRQASPKRPAQASAPAVKPAPRSNNAPGFDGPLPFEKLNLGKSLLGQKKMQKLEEDTAGGFEKDYGQDKQAAIPLPPWFKGQRDINEDLSVQRQDEKKQWEEAKAEDKARFQFQELPRLQKQETKDVIDLDAPASQSQKQVIALDSDDESDMYMEDVVEDNQLRMGDLADKLRAEPPKPLSPRQKSPPRQDPALVKKTPFADDSEDEPVEWSESEPEEESRLKAQENLPLQVTSPQNQPSRSPSEDFEDVPMQTEPAPAVEPPKSPSPEFEDVPMHTKKPHRPRRDSTPARLEGPEDLELPIVPHSGVADDNAPLELPENDSDQYSDPEDAELFASLAAEAEEHARFAQELNNNTTARINFDEELKQLRAQQKKDRRDADEVTQTMISECQHLLTLFGLPYVTAPMEAEAQCAELVHLGLVDGIVTDDSDTFLFGGTRVYKNMFNAAKFVECYLSSDLASEFSLTREKMIAIAQLLGSDYTTGIPGIGPVTALEILSEFKDLEAFKDWWIGVQNGSVPKNEDAKSAFRRRFRRGQATKLFLPTAFPDPRVADAYLHPEVDADPEPFQWGVPDLAALRTFLSTQIGWSWERTDEVLVPVIRDMNRREKEGTQANITRFFEGSVGAGAFAPRGSRGPSGKRKGTGAAGKRLGAALSRLAEREGARGGGVDDEDDDNAEGGTEVAAANAQVDGAARKKRKVGSKAASVSAEDEFDEAEDEDGDELYEEPKKKKPRKARKSKKTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPU3</accession>
    <name>A0A9W9CPU3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002648</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPU3"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018853">
        <property type="entry name" value="DUF2457"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10446">
        <property type="entry name" value="DUF2457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="446"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="707"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="751"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="256"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="514"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="539"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="551"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="622"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="708"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="727"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="766"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="775" mass="85968" checksum="E2B64E65C5AA6C58" modified="2023-11-08" version="1">MANEQDYINGPVALEAGSQRNISNTSLPATTTEEEVVADVDEPPEPARTPRPAPLKLHSSTTSFTKSTPILDRKESLLTSALKSNSNASSPVDEKSEQSPLRGMSCVSTWSNTSGTTAELTSDGGFTSPGTRTSTPSPPLPSAMFNRMMPTFNQKPLELPISIVSHDDENIDPLQKPTTSTNDEQKVEAVLGRKRCIMFACGKKEEKNPAVAAAKPTPPQAEAPPKRACTIKFACPTKTSTDTVPRSTKIRSRLVSPAPSARRIPSSPKPSSKVHRDSDSTICNNSPISVRKVAVVDRTRRLSANSDLARCEKYRFHEFASSEEEVEEWTQEVTCHRNRLTVQDTLKVENNLRQLGEEVEEEEALDEEEEEEDAADMDDDDDLLDEDDNDSDAYSDATDEGFQTDDEEGFAVSDDESDAGSDYNWWAPGASTAATSVEHIEQLRLKPHRTVSESSIGSLESREGLKISDKPPKRVKTRPVNIRIPSPELPDSTDFVCGTLDEDRPAEEEYMSTLKRRRAAKQKKTPQDIDPTFPTSDPELPDDEEDEVSEDEHVASESDFMMHGRMDLVDGNLRGRRTDGQKKRSPAPSPKRLRSPPPAKRAVHRSPPPRKLFGNSPKRLHSPPPPMRLRSPPPTRRGSVNPLAARQRSEMSIRFGGIGERPALMVSASVPRTPIAMMNPFDLEEEEVDPDEMPVRRAIDIQVGLERKRQRRKEQLYRKQHRKGVKEKRPAPGKGAERMREMGLAAHAHKGKTTAFGPFQIPANTDQKDMHVLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQC2</accession>
    <name>A0A9W9CQC2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001337</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfo/Idh/MocA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQC2"/>
    <dbReference type="OrthoDB" id="2129491at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43054">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43054:SF1">
        <property type="entry name" value="SCYLLO-INOSITOL 2-DEHYDROGENASE (NADP(+)) IOLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="3">
        <location>
            <begin position="142"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4376056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="332" mass="36359" checksum="56439AE0274855D9" modified="2023-11-08" version="1">MINFGIVGTGWITHSYVEAAHATGRWNLAAAYSRKAETAQEFASKYSDKKVATYTSLPDLAADSNISTVYIASPNIFHYDQAKLILNAGKNVILEKPSCSTLAELDDLFATAKAKGLYLIEAFRHIQEANFKLLKASLPLLGPLHGASITFAQYSSRYEKVLAGEVPGIFDLNMGGGALVDLGVYCVAAAIDLFGAPQASTYYPVMLPTGADGAGRLILTYPTFTVHLCHSKIYNSEAPSEVYGEKGTLIIPTITDVERVTFWDPRSKQKRVLEGVRKPEELNLKEEAAEHARIIEERDEAALKRLEQHSRDVLSVLEKARHNNGLLFPGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQG5</accession>
    <name>A0A9W9CQG5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001488</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQG5"/>
    <dbReference type="OrthoDB" id="10251048at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000069">
        <property type="entry name" value="HAD-superfamily subfamily IIA hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050324">
        <property type="entry name" value="CDP-alcohol_PTase-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006353">
        <property type="entry name" value="HAD-SF_hydro_IIA_CECR5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01456">
        <property type="entry name" value="CECR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269:SF4">
        <property type="entry name" value="CAT EYE SYNDROME CRITICAL REGION PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4376206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="430" mass="47753" checksum="9C7DD6D465B1C537" modified="2023-11-08" version="1">MSAPSFAIPQAGSRYVRRLSSIEATRENITMGPTVVVPPQHLMAPSSASEIASDFEKARHHLESAATPGSPGTSPIETSVTDKYAFAFDIDGVLIRGGRPIPEAIEAMKMLNGQNEYGIQVPYIFVTNGGGKTEAERCVQLSKQLEMEVSPGQFICGHTPMREMAEKYNTVLVVGGEGEKCRIVAEGYGFKDVVTPGDIIKDNQDTTPFRKLTEDEYTNSRARNFAEVEIEAIFVFADSRDWASDQQIILDLLMSKNGRLGTRSANYDEGPPVFFSHNDVVWSASHDLTRIGMGALRVSLEAMYKAVTGKELQTTAFGKPQIGTFEFATRLLQEWRKETHKIDSPPSTVYFVGDTPESDIRGTNEYNEHTDEANWYSILVRTGVFQEGTKPRFQPKATVDTVLDAVKHGMEREYKKQLKESMLKTGVIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQG8</accession>
    <name>A0A9W9CQG8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_002072</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQG8"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4374993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="326" mass="33821" checksum="5338D42A052B5834" modified="2023-11-08" version="1">MLDADGEVKGIVEAMGDPVANVTGELVEPDDPSVKGKLLEADNMLDGRLEKKGGETVVVTVDIRELNESAPEFNPLVIDGSRTVVVGALMVELGLDVAPVVLSIDRGLVSLGLDVGVLALDVAVLKRPVNLDVSRPLTDEGSVEGPDKVGEIPGVASDSLGLVNVGISEEPAICVVDLDDRLEPIGKKDEEELALFDDPDNPGLVTDNDRGIVVRPPVLGADNEDASVDRIVRPEVMPVIVVVSAMEMLVIAEVPDPADGFGKEIVNPPLPSVVIAVDVTDMLGAGVVSGVRPLSRVVGVPMSVVVPPPGIVLVKVISVVDVIGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQT2</accession>
    <name>A0A9W9CQT2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7025 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002453</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQT2"/>
    <dbReference type="OrthoDB" id="10042665at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054289">
        <property type="entry name" value="DUF7025"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46411:SF2">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46411">
        <property type="entry name" value="FAMILY ATPASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22942">
        <property type="entry name" value="DUF7025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7025" evidence="1">
        <location>
            <begin position="158"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="426" mass="49247" checksum="A66F4D0ABC0EFB41" modified="2023-11-08" version="1">MSTGAYDCLYQTLGSGPPIVKPKFEDVTVTQDIYGNVESTRRTAESLKYKVEKDRYNDIAILIRRRLSSTREALWIQMEIQSPDIQETVTKVCGHSDLLNTKVSPIVIRKPYLILFHYRAELREYDLHSDRTDQEKVHLKVLVDFMAKAFTRDEAEYSRLIDKGQVSFPIVWTLFKPEEEVFIHESHFVRCGRVQSLHGINKTQGWSLGTRSWDYDGTFFGPVDSVSPLQPFEGICDITSLPVYPVRFHKNQNKDDLRQRLIERGRKWKSMVDVTHLSYEGSDDNSVQLQVSSRIITDYATHQQANPHHATAFSWYNASLTKQTKEPPADDGNDDGDDVDDAERQTQALEKEDKEAQKEHDRFFLQRHDSSYIMSDDQALLCPAKIRGFSLMDKKWAFFLVENATDIQWLENPMERLEIDGAPKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR82</accession>
    <name>A0A9W9CR82_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Transcriptional regulator of nonfermentable carbon utilization</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ERT1</name>
        <name evidence="12" type="ORF">N0V83_000549</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERT1/acuK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR82"/>
    <dbReference type="OrthoDB" id="2538135at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050335">
        <property type="entry name" value="ERT1_acuK_gluconeogen_tf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056751">
        <property type="entry name" value="PAS_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47659:SF1">
        <property type="entry name" value="TRANSCRIPTION ACTIVATOR OF GLUCONEOGENESIS ERT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47659">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24990">
        <property type="entry name" value="PAS_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="11">
        <location>
            <begin position="65"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="169"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="392"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="547"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="178"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="197"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="392"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="550"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4377720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="694" mass="76477" checksum="A922F4D16B026FED" modified="2023-11-08" version="1">MSTPGAEDASPSPEYSGDLDDDMAAEQTSDRQSGDASPDQKTTNGKIASNAKDPLRPRRKKARRACFACQRAHLTCGDERPCNRCIKRGLQDHCMDGVRKKAKYLHDAPDGALMPGVGGHYPYMNGNRPTPLPSQDTQTASVTPQGNLYTQAPPTTYYTPNSVPGSLPVNQDGRTFNSQQSPISPPFSQAHHGSVHTAPSTMPQGQSGQMPQFGPLFDPSDPALFNFDISSLNFGNHYGALEFGMLGHMSSGAAEQAHDNSLMNPMSQAANLYNQQMPASYADQSNPAVAMSFGPNGLPTGEWQESPTRQGNMQVQTPNTPATAHLDHGNHRNDSINGPHAFAIGQGPASHSTASPASTDASAFENDNPLASAAFFANTNRPHVQRSPVINRSQQENRPPNTALQPIHSNGIRKRQRDTKSIYQGITKPYDYVKGYHRLYQLINKKYSRPWVAKAQEYLQNYRPVLLQVREELNTDDLIHQEMGLQRNLMTLQEHFAEVGTPFLICRRSGEIVGINKEFTILTGWKREVLLGHEPNLNVNLGANRDASDSELSTQNTTPILPPQDGETGTSNVNIIELMDAKSALEFLQHFSELCYQDPRGYASQRVNMLRYQTKADFDRIQDMKANAATDVKLDPLVKMEGGAVHQGESAMQRLGAKNGMVDCMIWWHIKRDIFDMPVLVCMSVMPVLDKGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRL0</accession>
    <name>A0A9W9CRL0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRL0"/>
    <dbReference type="OrthoDB" id="429813at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051414">
        <property type="entry name" value="Adenylate-forming_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43439:SF2">
        <property type="entry name" value="ENZYME, PUTATIVE (JCVI)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43439">
        <property type="entry name" value="PHENYLACETATE-COENZYME A LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23562">
        <property type="entry name" value="AMP-binding_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="320" mass="36255" checksum="BF2AFDA00937ADE6" modified="2023-11-08" version="1">MSGILLWPVLNDSPTILPPSDSIGLPPDVFKKIMALTPAEGIKCPPHTIHQLWEDAESKKLLKSLEFITYLGAALDQAIGDELCQHVRLSPLIGSTETGDQMSICPLDRKLWYTHSFVPENGSEMVAVEGTNGLHELILNLRKDASTKQFQSSCWNPAFKGLKRVETKELYTPITDRDGETRWIFTARKDEMTKLNWLAKFHAHDIESRILRHPDLQSVLVGGEGRPVPFIIVEAKQSALNGRSEEQLLDSVYESVVVDTNKTDTAEIRIPRETLILAKTEKPFKRNQKEVVLRREVEKDYAHEIEAAYRKFVERKSSTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRS1</accession>
    <name>A0A9W9CRS1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_000203</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRS1"/>
    <dbReference type="OrthoDB" id="5426798at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050815">
        <property type="entry name" value="TF_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47338:SF11">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47338">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="75"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="113"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="143"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="722" mass="81220" checksum="1A91B2FBE2535D71" modified="2023-11-08" version="1">MIYLAKVIGQHQQHLTQQQPSQTIQSKMVSEMLVDRLEDALMDGTYCRAIIDGEPVNPSWGITKAGKPRKRLAQACLTCREKKIKCEPGYPKCHQCAKSQRVCRGGLNQAGMSNASGDTSPSSSAPLFKNPSTDVLSPGAGSDKTKAPGELREPSKKFDSWNPVAQFRSRSFRPNSVATSRDMSVHSVDSDWSGSANDQGIEDARRGTYQDLLALQWEQDPYETDPRLTMHLLDLYFLHAGRATYGMFPRRPFLAWAETDREKSQDHLMLLYSVLAMGSLFSSDPDKIALGKRFAAVASYAAEKRFGRFSLQLCQCRLMLALYYFARGKSQEAWDFCGAGLRAISALKLNTEEGVKELVESIPDLDYGFDRWTYEECCRRTFWSGLLMDVSHVLSEARSSIDVVQQQRYNGFFGGSLFVISIEDAYVRLPCPDNMYEASTPCETPFFDEDLLSGQLSTGPVLGDMAYLCIISALWGDVLTFTARAVRRPEATYEQHYESFYSRTYEKLEAWHSMLPANLRYSPQNLDNSIVEGYAGTFLSIHALYNAVIIRLNRHVRVRAMSTERLRRNIEQAFRYSTNFLSMMHSLAAVNRQQHLQPNIASGFLFSTPFPGYALMLSIDVLTSAGIYPTLPNLIETISATMSCIDELAGFWASAKAQQKVISSRLKQLTDVAVQENQGVSNGSYGRYWKLNDSLETVFGNEDAIYKAEDRFLFDVVGQRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXB4</accession>
    <name>A0A9W8XXB4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent DNA helicase II subunit 2</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">ATP-dependent DNA helicase II subunit Ku80</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">YKU80</name>
        <name evidence="22" type="ORF">N0V83_010986</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Single-stranded DNA-dependent ATP-dependent helicase. Involved in non-homologous end joining (NHEJ) DNA double strand break repair. DNA-binding is sequence-independent but has a high affinity to nicks in double-stranded DNA and to the ends of duplex DNA. Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection. Required also for telomere recombination to repair telomeric ends in the absence of telomerase. KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance. Interacts with telomeric repeats and subtelomeric sequences thereby controlling telomere length and protecting against subtelomeric rearrangement. Maintains telomeric chromatin, which is involved in silencing the expression of genes located at the telomere. Required for mating-type switching.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ku80 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXB4"/>
    <dbReference type="OrthoDB" id="30826at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043564">
        <property type="term" value="C:Ku70:Ku80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00873">
        <property type="entry name" value="KU80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.290.10:FF:000008">
        <property type="entry name" value="ATP-dependent DNA helicase II subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000073">
        <property type="entry name" value="ATP-dependent DNA helicase II subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1600.10:FF:000002">
        <property type="entry name" value="X-ray repair cross-complementing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.240">
        <property type="entry name" value="Ku, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006164">
        <property type="entry name" value="DNA_bd_Ku70/Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024193">
        <property type="entry name" value="Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036494">
        <property type="entry name" value="Ku_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005161">
        <property type="entry name" value="Ku_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014893">
        <property type="entry name" value="Ku_PK_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604">
        <property type="entry name" value="KU AUTOANTIGEN DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604:SF4">
        <property type="entry name" value="X-RAY REPAIR CROSS-COMPLEMENTING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02735">
        <property type="entry name" value="Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03731">
        <property type="entry name" value="Ku_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08785">
        <property type="entry name" value="Ku_PK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016570">
        <property type="entry name" value="Ku80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00559">
        <property type="entry name" value="Ku78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101420">
        <property type="entry name" value="C-terminal domain of Ku80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SPOC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 22" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="VWFA" evidence="21">
        <location>
            <begin position="6"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="569"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="572"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ4362044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="733" mass="81977" checksum="09390D819C037C71" modified="2023-11-08" version="1">MAGKEATVYIVDLGRSMGEKRHGRDQTDLDWALEYVWDKITTTVATGRKSALMSVIGCRTDESDLAGVMELADGYGNIRVFSPVKQYLLGDIRNLQEHLKPSKTNDGDLVSALAVAVQMIDGATQGAKGNPLKYDRRVIIVTDGRGNMDTDDLEMIASKIKDPSAPIEVVLLGVDFDDADSGFKEENKERPKAENEETLKTFVEDCNGNFGTLAVAIDQLHTPRLKETRPVPSYKGQLTLGDPEKYDATITIDVERYPCTMLAKPPTASSFVVRSDLGGVPGPSEQSSATIVGEDHPMSDLSAVRNQRVYQVDNQEEPGTKKNVEMDELERGYEYGRTAVHISESDMNIVKLETQPSLSLVGFVKAEEFERYLPLSRSNFIVPQRTNQQAQLALSSFVHALYEAECYAVARMVTKENKPPVLLLLVPRIELDWEALVDVELPFEEDMRRYKFPPLDRKLTISGKTITEHKDLPTKDLMNAMSDYVDAMDLSTFGRDEDGNEEEYAKIEDAFSPLVHRINHIIRWRATHPDPTLQIPDPPEILTKYSAPPAELLAQAEKQLSALKKAADVKKVPPKVKGRGKRQRTDRDKPLSGLDVDELLGNPKRVKIETNNLVPSFKQALAASDDLEAIQEAANGIAKEVRSLISSSVGDSAYNRALEAIRVMREEMTELEEPEIYNAFIRDLKLDILAGKLNGNRRDMWWKIKGSKYGLVDQKRSLVSDVTEEEARKFYES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYJ0</accession>
    <name>A0A9W8XYJ0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Mitochondrial dynamin GTPase Msp1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MSP1</name>
        <name evidence="10" type="ORF">N0V83_010123</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYJ0"/>
    <dbReference type="OrthoDB" id="10254455at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140570">
        <property type="term" value="P:extraction of mislocalized protein from mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051701">
        <property type="entry name" value="Mito_OM_Translocase_MSP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644">
        <property type="entry name" value="AAA ATPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G12920)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644:SF3">
        <property type="entry name" value="FI08533P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase AAA-type core" evidence="8">
        <location>
            <begin position="146"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="AAA ATPase AAA+ lid" evidence="9">
        <location>
            <begin position="294"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4363005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="371" mass="40963" checksum="23D29A57EC06D412" modified="2023-11-08" version="1">MSSNKKSSLTGYLPDILMAAAAPLIAYFVIRNLLSRLDPEAQQKEEARAKASAANRKLDAILTSKRRKSYGEYDSEDEETERGSRRPRIQDLNLSTYEQTIAMEVVAPEEIPVSFDDIGGLDSIIEELKESVIYPPYHAPPLLTLTMLAKALAHESGACFINLHISTLTEKWYGDSNKLVNAVFSLARKLQPSIVFIDEIDAVLGQRRSGEHEASGMVKAEFMTHWDGLASSTSSGSSTPQRICILGATNRIQDIDEAILRRMPKKFPVALPNASQRHNIFSLILRGTKIDSQNFNLDYLVRVSAGMSGSDIKEACRDAAMGPVREYIRRKKADGTLRHSSRRGVEQGDVRGLRTEDFFGRGEGVEGAGCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZS1</accession>
    <name>A0A9W8XZS1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Scaffold-type E3 ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">DCN1_2</name>
        <name evidence="2" type="ORF">N0V83_010619</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZS1"/>
    <dbReference type="OrthoDB" id="27198at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="158" mass="17577" checksum="E8FB5CADED40FBF9" modified="2023-11-08" version="1">MIHLRALARCILCSSTRTEIDNNPHELPSRPKAKDPREGPCTPEQDPHSRLTSQPGATLEALQRVVRDTDGAEELNELMPPKRKYHDAAIADMARPQDKKAKTTPKKGKKATAAAEDVYSSHQKAAIAQFISFTNIDRNTAIRVLKNNGWDTQNAVNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y009</accession>
    <name>A0A9W8Y009_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_009838</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. MetX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y009"/>
    <dbReference type="OrthoDB" id="191364at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004414">
        <property type="term" value="F:homoserine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009092">
        <property type="term" value="P:homoserine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00296">
        <property type="entry name" value="MetX_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008220">
        <property type="entry name" value="HAT_MetX-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01392">
        <property type="entry name" value="homoserO_Ac_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268">
        <property type="entry name" value="HOMOSERINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268:SF11">
        <property type="entry name" value="HOMOSERINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="292"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="475"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="544"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="707"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="486"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="547"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4363542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="739" mass="82001" checksum="3620BEC5AAF16695" modified="2023-11-08" version="1">MEFIEGIQLDNSKWLDLGEKAQKIICSKVSKQIQQLRSIPSEGYYGRVHNQGWKPNHPFLRTRRESMCGPYKTYEEFVLAMHTSAEVEAAMGLGFLKPQDLLLLDKFKSALATWEPHEPKLTWLDCRLENLVVRPNEGVGGDIDDWDVVLIDWAECGWFPAWMQAVGFERHWEIVDKNFDPDYPKTKMCMQIFLEDLEKEPSREEMNLSRGVRKSTSLASVLIPLFAKENSFGTNNKIKTVSSQPENPFAELIPDQTFAIIPEVTLESGGVLHNVPVAYKTYGTLAPAGDNAMVICHALTGSADVGDWWGPLLGGPGKAFDISRFFVVCINSLGSPYGSASPVTARDGDASLGLYGPEFPLTTVRDDVNIFKLILDDLGVKQIAAVIGGSMGGMLVLEFAYFGKDYVRSIIPIATSARYSAWGISWGEAQRQSIYSDPKYDDGYYLYEDPPSTGLGAARMSALLTYRSRDSFESRFGRNTPDLSKKQSINTVPRPMTPSNPSHENWAIHNHGHKNAGSPRLVSRTNSSAILPVSAAAQVDLEFHDASNPSTPPTQGTRTPTSDGSFSKSTKLAPHYFSAQSYLRYQAEKFIKRFDANCYIAITRKLDTHDISRYRYPEGQSPETLDPVSALHHALSLIEQPTLVLGIQSDGLFTFAEQKELAAHIPNATLKTIDSPDGHDAFLLEFEQVNKHLLGFLKSVLPDIMSRTPNQETDTTDSGMAATRTSTFGEAEVDDITAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0H1</accession>
    <name>A0A9W8Y0H1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_010302</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0H1"/>
    <dbReference type="OrthoDB" id="72053at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052250">
        <property type="entry name" value="PDI_TMX3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46426">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE TMX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46426:SF1">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE TMX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040945724" description="Thioredoxin domain-containing protein" evidence="7">
        <location>
            <begin position="20"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="639"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="27"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="226"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="177"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="205"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4363182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="707" mass="79620" checksum="079C494E264A8CE4" modified="2023-11-08" version="1">MRAIFYSLLSLATVATTLAVDTKSANKAAADDDWDEKEPDTIFNGQTVPPMIELGQHNIDKEISKGNWMVEFFSPKCPHCMHFKPTYQTAYEFYYTSKPILTKEDSEGSSLNDFTRFYDFKFAKVDCIAFADVCEAHTVQNYPSIIYFEDGKEVQREKGAKDMKALSKWVEELLEKIRPGSRKEGGPKLPKPGANSVETGPDTEEAIKELEKEEADDKKAPKSTPTQAAKPTPAKAKPTSKANPSGLVETLTAEKFQDVVTNTLDPWFIKFYAPWCHHCQALAPTWSNMARQMQGKLNIGEVNCDVEKKLCKDAQVRGYPTMLFFRGAERIEYEGLRGLGDLLDYAEKATAVGAGVLDIDAEDFKKLEETEEVIFVYFYDHATTSEDFQAVERLTLSLVGHAKLVKTNDKALVDRYKISTWPRLIVSRDGKPSYYPYTTPKDMRNTSKIVTWMKSVWLPIVPELTSSNAREIMDGKLVVLAILSRDRSDELILSRRELKSTALEWIDKQETAFQLQRQELRDAKQLRIEEAEDKDDQRALRNAKSIHINMDELERAQVGFAWVDGVFWERWIRTTYGIDVKDGERVVINDEDSRLYWDTTANGEPIRLSRTAIVETLGKVTSKPPQITPKSTTGSFMSLYLSLTSFAGSHPFLALGMVVGFFTAAVLFGRSRSRRRTFGNTGAYFQLGEKDGLLGGLGSGNGGAKHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0Q1</accession>
    <name>A0A9W8Y0Q1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008586</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4364970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0Q1"/>
    <dbReference type="OrthoDB" id="3353407at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021822">
        <property type="entry name" value="DUF3405"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36205">
        <property type="entry name" value="CHROMOSOME 19, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36205:SF1">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11885">
        <property type="entry name" value="DUF3405"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="573"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="587"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="833" mass="95047" checksum="6F7B73BEF1C7E0B0" modified="2023-11-08" version="1">MLGFRPLSVGKRTPQAYHQAPIYDPDIEDGADNEKGYSSDDDNYVSSPGPSPYSSRQSSSSYDVNPNNIESRPLNPNSSHRRAPSRPRFSAYSYRNPRACTRYFGLAIASTLLFFIWFLFSSSWESIRTAELGIHKPPPPPRVWESFPFLKRYHGGIRTLVTRDKNVPEYPTKQDIDPEMPKPEATGAPVEKRSQGAHIPDSVPFDPYPEYSELTYVEEYGPVETCYLDANDTIKIPGVRAYKGVTDGMPENVMGSYSLLGLRNDVCFERFGRLGPYGMGYSKRSGGTGAGMEGDREGIDKVWKANPEVDFRELKWTDVLDRCLSRNKGRFQTQPKTSEPSFQTMAMHRRDTVHNTTSTRNTTTVHIDQTVREQPKAAYNKLIPRTAVLIRTWSDYSYDDEDIMYLRALISELSIASGGEYHVHFLIHVKDDNKQIWADEKVYQEVLEAALPSEFAGMGTLWSERQMGLIYGGIEDSNYRSLPVHGVYRSTYMPVTYFAHQHPEFEYFWHWEMDVRYTGHYYHLFQQISKWADAQPRKGLWERNARFYVPSVHGPWEDFKHMVRVQTEHGTNNQANRWSSHLPPNPHVKETQVQKPEKPIWGPEPPQDYDGIELDPSVQPNTTMLEDNYVWGVGEPADLITFNPLFDPENTDWILSDDITGYSKEKGLPPRRVTINTSGRLSRRLLLTMHREQSHFRHTMSSEMWAASVSLHHGLKAVFAPHPVLIDRRWPTQYLAAIFNNGRNGASGGARMSVFGQGREHNLLGTTWYYNAGFAGNLWKRWLGYKVDGDGGEVEELGGEGRMCLPGVLLHPVKQVDLVQEKVDVGLDPDVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0S6</accession>
    <name>A0A9W8Y0S6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0S6"/>
    <dbReference type="OrthoDB" id="2159786at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070860">
        <property type="term" value="C:RNA polymerase I core factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001164">
        <property type="term" value="F:RNA polymerase I core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001181">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053029">
        <property type="entry name" value="RNA_pol_I-specific_init_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007224">
        <property type="entry name" value="TIF_Rrn11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28244">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28244:SF1">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04090">
        <property type="entry name" value="Rrn11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="454" mass="51124" checksum="203F73DBE93D4184" modified="2023-11-08" version="1">MQYFASQLRGPQEPKQSLRAAQYRSKRKAKHDDAEEDLSTSPDSEVKTRPSSRAYSHASFVSSEIAQLRVAGLLPEDEYQVPPAPFPHAPARVSKGHYGSIGAEKEMAKPPSRLYAINATSKGDSDDRQTASNALKRTHLNVLSTVLHRCLLEGDYMRAGRAWGMILRTQVAGGHPVDPRNQGRWGIGAEILLRRKQPNVAGQNEQAQAASREVEVEVFSEQGFELAREYYERLIVQHPHRRTHPNTIDERSFYPAMFSLWILEICEKSRRARKNVEDEAARSRSLRSASIGSVLDDPSNGLQAKEKAFHVEELARAREIAQRLDQLIISPPFDKEASLFQLRGNISLWISDLILGNDTAIDEDWSMDSVVRKEEGNSEGATESLMRLTNCHREVQQAQGFLQRAEANGAHRQSSTLSSIEIKLKELSWRIEKLRTQQEAGFSSLNTLDDRMSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1K8</accession>
    <name>A0A9W8Y1K8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">amidase</fullName>
            <ecNumber evidence="3">3.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_009549</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a monocarboxylic acid amide + H2O = a monocarboxylate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:12020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35757"/>
            <dbReference type="ChEBI" id="CHEBI:83628"/>
            <dbReference type="EC" id="3.5.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.4" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1K8"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1300.10:FF:000003">
        <property type="entry name" value="Amidase signature enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072:SF10">
        <property type="entry name" value="ACETAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072">
        <property type="entry name" value="AMIDASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="7">
        <location>
            <begin position="63"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="5">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="208"/>
            <end position="211"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4364094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="564" mass="63020" checksum="D91874CB55DC2B0F" modified="2023-11-08" version="1">MGLLEYRQHKLDCQRKQSERASKIATLPTQYLYPLSPQERTILGRPIQELVQDVQKQITSPVDILRAYSKVAIKAQEKTNCLTEILFPEAEEWAEKEINLKGPLAGIPVSLKDSIQVKGFDSSVGYSRNVGKPYAEDGAMVKLLKDAGAIPFVKTNLPTTLLSFESTNDVWGQCKNPHNDKYSPGGSTGGESALLAFGGSRIGIGSDVAGSVRAPAHFSGCYSLRCSTGRWPKVGMNTSMSGQEAIPSVFSPMARTLNDLTYFTRSFVQMKPWKYDYTVHPLAWRDDMETEYKEKKKLRIGIMRTDHVVDPAPACARALDETAKALAAEGHEVFDVEPPSPYEALYLASQLLLSDGGNTFMSHFRTGEWNDLGAAQMMKYMRLPRFIKYCHYLWVKYVKGDSIWAGLLRDWHPKTAHEYWQLAGKREAYKARFFDWWSDVAQMDVMLTPPNATPAVPHGGMHDAVSSCGYTFLFNLLDYTAGIIPVTHVDPLKDALPSTFNFKKLNGVAQGAYKHYDAVKMAGLPVAVQVVGRRLEEEKVLAIMERVEDALDKHGGKYQLLEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1N2</accession>
    <name>A0A9W8Y1N2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008941</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1N2"/>
    <dbReference type="OrthoDB" id="194443at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4365321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="176" mass="20514" checksum="8CC7463F1CFE834C" modified="2023-11-08" version="1">MFEGKDITAPERVRELTRNLGNLCAGKDQLFRLKLCILADRLVADVFAHAANHCFVDHVLREQHVDYAVVIHAWKPWLPPNHPIMDFLVDAQCATGRRDKDTAANGRLALREQLPNGFLLKVMDRGPIINTDQRSLLYRCDYHDHVSEQERKDCEARRRGRETKLEAQYWTYEPDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1T9</accession>
    <name>A0A9W8Y1T9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycoside hydrolase family 32 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_008286</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1T9"/>
    <dbReference type="OrthoDB" id="202537at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004575">
        <property type="term" value="F:sucrose alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005987">
        <property type="term" value="P:sucrose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18621">
        <property type="entry name" value="GH32_XdINV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.560">
        <property type="entry name" value="Exo-inulinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001362">
        <property type="entry name" value="Glyco_hydro_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013189">
        <property type="entry name" value="Glyco_hydro_32_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013148">
        <property type="entry name" value="Glyco_hydro_32_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42800">
        <property type="entry name" value="EXOINULINASE INUD (AFU_ORTHOLOGUE AFUA_5G00480)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42800:SF3">
        <property type="entry name" value="GLYCOSYL HYDROLASE FAMILY 32 N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08244">
        <property type="entry name" value="Glyco_hydro_32C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00251">
        <property type="entry name" value="Glyco_hydro_32N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00640">
        <property type="entry name" value="Glyco_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040865087" description="Glycoside hydrolase family 32 protein" evidence="5">
        <location>
            <begin position="21"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 32 N-terminal" evidence="6">
        <location>
            <begin position="54"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 32 C-terminal" evidence="7">
        <location>
            <begin position="467"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4365666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="643" mass="71477" checksum="ABACC7DD7D1280A2" modified="2023-11-08" version="1">MLWSIPLLSLALGYSPFARAQNDTESELPAILTANIIEALGNNSLFTRWRPTYHFISPAGWMNDPCGMMYDPTADTYHLHYQWHPNHVNWGNISWGHATSKDLITWTDVGGWENDQAQSIGTGPTPESRNTSYYGLGIFSGSGQPYNLEGEQNGTLIVFYTSVQYLPTSWSIPYTKGTEKQSIAISNDGGQTWEQYEGNPVLSSPPEGWNITGWRDPFVEPWPEMDALLGQKEPHWYMVLGSGIKGEGGGGRIPFYSAPSSNLTDWTFLGALWEPAQNETLGSLLETGTYGFNFEVSNFFSLTDEDDDVHYYVLMGTEGGNLTWHPRAQWGLWNEGLVSRRENGSVDFTPVAGGAIDSGLLYAVTSFNDTKHNRRVQWGWANEDNNNFAINQQGFQGSMAIPREMYVIKTRDLVNTDGGLTTKGNTRVVEHGNGTFTGYTLGARPLPDVVEGLHAGAEPQNIDTVDTWTTSLPAGNGSSHMHLQVTLSNFTGPAGVTIAMSPGREEQTAIWYSPENYTINVDRSQSSIVGTEVSNYTMLGYFYPYTFSNGTQESLHMDIFVDGSLVEVIVNDRFWLTTRIYPARIDSTGFGIWVGDNSTVQVTNMTMWDISTNVFPDRPVNSSSELVFDTPEETNNYVWWTGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y265</accession>
    <name>A0A9W8Y265_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y265"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="275" mass="30648" checksum="EED88276CA1DE7C8" modified="2023-11-08" version="1">MATVPLGRDAHSRMIGLPSEEVARVHLEDDINAESDVLNRSQELRERASEADRETRLLFEELPASLPDVEEPTITAPDGDTGASLSVDNSTGSTPPLKDAPEDLTGLQSNAQFDLESQYSDEPSAQEAINEVTRVSRGTQTSYLGRWERWGVLQLAKEDAYERGYLAGREEQRLQNGVEQSTQTENPKSLTTGCTQTSPQPLEKTKGPETQRDVKKNRYIGQKNDITSGMERLEREMAEISDNEDMHYVPKKENREPTEDPFKALLGLVRTSKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y299</accession>
    <name>A0A9W8Y299_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y299"/>
    <dbReference type="OrthoDB" id="10564608at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="325" mass="37558" checksum="475EC9F26E244781" modified="2023-11-08" version="1">MATYIPAYLWLDDSDDSASDGEVVPRVRAPLGRAARDTLAKLKERTPAHIREVRDYFYDLEECKHKGIPGVHDKNDFFRLVGILITMPDNFVQEILARVKGKGDTTKKLKSPTAKMLVAKLSDRNDKFVDEVYRLWFILVQKRNGDDEERKRMFKDIGLLLQIPELEVHEMIWTLLELIETGENAEDGAQRLQGPGPSITTRAGKYRKRPRHRHPKSLGTLSLEDAPSRPAPSSPAPSRPAPSRPVGKKRKRRAEVSERRVEVGDWDWDGRLTEPHHFQRLSVAIWQCRVCDLWNRRDQARCERENRDTKVVCRGKRENALRTRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2F7</accession>
    <name>A0A9W8Y2F7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AA9 family lytic polysaccharide monooxygenase</fullName>
            <ecNumber evidence="1">1.14.99.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Endo-beta-1,4-glucanase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glycosyl hydrolase 61 family protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008955</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Lytic polysaccharide monooxygenase (LMPO) that depolymerizes crystalline and amorphous polysaccharides via the oxidation of scissile alpha- or beta-(1-4)-glycosidic bonds, yielding C1 and/or C4 oxidation products. Catalysis by LPMOs requires the reduction of the active-site copper from Cu(II) to Cu(I) by a reducing agent and H(2)O(2) or O(2) as a cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[(1-&gt;4)-beta-D-glucosyl]n+m + reduced acceptor + O2 = 4-dehydro-beta-D-glucosyl-[(1-&gt;4)-beta-D-glucosyl]n-1 + [(1-&gt;4)-beta-D-glucosyl]m + acceptor + H2O.</text>
            <dbReference type="EC" id="1.14.99.56"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Has a modular structure: an endo-beta-1,4-glucanase catalytic module at the N-terminus, a linker rich in serines and threonines, and a C-terminal carbohydrate-binding module (CBM).</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.56" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2F7"/>
    <dbReference type="OrthoDB" id="2525337at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030248">
        <property type="term" value="F:cellulose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005103">
        <property type="entry name" value="AA9_LPMO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03443">
        <property type="entry name" value="AA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Auxiliary Activity family 9 catalytic" evidence="2">
        <location>
            <begin position="21"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU368122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="72" mass="7801" checksum="10A20EFA6F135FCC" modified="2023-11-08" version="1">MYQGTFKGVRIPAYNGAAGKGGYNNSPVKDLDSIDLRCNVMGDVQAHDTIKVAPGDNLTFDWYEIMASSLTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y330</accession>
    <name>A0A9W8Y330_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lethal(2) giant larvae sro7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SRO7</name>
        <name evidence="5" type="ORF">N0V83_008454</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat L(2)GL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y330"/>
    <dbReference type="OrthoDB" id="19944at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045159">
        <property type="term" value="F:myosin II binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000848">
        <property type="entry name" value="SNARE-dependent exocytosis protein (Sro7), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013905">
        <property type="entry name" value="Lgl_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241">
        <property type="entry name" value="LETHAL 2 GIANT LARVAE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241:SF25">
        <property type="entry name" value="TOMOSYN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08596">
        <property type="entry name" value="Lgl_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="230"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="459"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Lethal giant larvae (Lgl)-like C-terminal" evidence="4">
        <location>
            <begin position="517"/>
            <end position="902"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4365833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="944" mass="102383" checksum="2EC3FE8CB10AB785" modified="2023-11-08" version="1">MAHLLRGKQAGVNHDLSIGIGADLFVLDHIRNFGINSKVTQVAYDPVQSLIAIGTSESQFGNGQIYVFGQKRVEVVLPLPHRASVKILQFCTEKLLCVDSKNDLSVYSLESKKLVNAHSPPAKITALHSDPTIDYALLGTQHGEVFAYDLDREILTPFKIPNLWREKFPRSRLTSVVTLALHPRDIGSLLIGYNAGAVLYSFKQNKALNFFNYVLPKGAPGGDSDPGSIFKEREPPLTQAVWHPTGTFVLTGHEDGSIVTWDPKDGRVVQARTLQDTNVDKPGPGSFSPGAVEGSFALKSPIFKIAWCANQDPDDTAILIAGGQQSNISAKSLTLFELGRTPVYATSSWQILSGHFENPKRQRILPCPPGAEVVDLCLIPRSSPHYAGCHDPIAIIALLASGELITMSFPSGMPISPTNQLHLSMTLVHPYIHYTNLAPVERTKWLGMTEKRQQGPPFLNGGAGAIAPLKRFEHRNIVQTAHADGTVRLWDAGHADEIENEALLQIDVARAVGRHDNVEVARTSFAGAASELSVGLKSGEVAVFRWNVNKHPGQEFPPGENQPQALTNITERTEAALKEGLLPFTLLDEGNGPVTALKHSDVGFVAAGFEGGSLAIIDLRGPAIIYKGSIQDFVKSDKRGSFRKSASQTTTKPEWPTCIEFSVMTLEDEDYSSISCHVGTNMGHLATFKLLPEAGGRYSAKFAGVCLLDDKVIKICPMYAETGRPAYASQSAVAGLRNGSKIKGVLLAVTASGARLFHPPTNKGAHKTWDQFLCDSAAVVRYEELGYALLGLYGDGCARAYSIPALKEIGSVKVSDVLDIRRFSEAVITSTGDILGWKGPAELALINVFGTGLQFDRAKDILMNPDLLIPPRPTISNMQWISGTQYVTPADMDLLIGGPDRPLKTHARPNALRSRTTTPRHCTLLQPISGLLRVPFLSHSTCKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3A1</accession>
    <name>A0A9W8Y3A1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008954</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3A1"/>
    <dbReference type="OrthoDB" id="417877at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051104">
        <property type="entry name" value="FAD_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46720:SF3">
        <property type="entry name" value="FAD-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46720">
        <property type="entry name" value="HYDROXYLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01460)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17164" checksum="B72BB8BE27C89D02" modified="2023-11-08" version="1">MAGEYLPQIREALSRVPPGGWREFAAFAGPELDTLTAWNNKIVLVGDSSHALSGAFGSGAGFTMEDGWVLAQSLSYSKNDLSKALPVFNAIRLTYYSRMYAHLASEAKRRAAKLKTLGNPSFDERVASKIITDGGVDMSWIYEKDISATWRQSVGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3I6</accession>
    <name>A0A9W8Y3I6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007819</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3I6"/>
    <dbReference type="OrthoDB" id="3021074at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="422"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="489" mass="53826" checksum="587DFC60DA159B1F" modified="2023-11-08" version="1">MCDFPESRMVAECLLGCSVGAARHSGVTRPRAGFRAYEWSVSSNTQDFYEQPYNSHILGVVDMDLYGAFQICSIGILAAPLTVRLSRTYFYDPGRNIIFLWTILILSGLVALCVEFYRVTPTDCKTDNFNHTISSAATFPYGGNATCGLLCSETDGPFSVLRGGAAANVNVIPVPRILTFNALMLLSAGFCIPAILSLVFTWDKILEINWKRQREDERLNERIEGATITVGEMRGINKVVRLFLSVVEIPLFGGVILTIIGIGEANFFSPQVNYDTEPMGSIGGEDITLQSRTQHRYSESSHRNRSNERDVSPGRPSMQQRSPSPMEIRSTSPHDIGLIPTITEPDLAHDQDDDAQSHQSEPTAGRNKVRKWLTSAANSLGNAAHDKLDVSGYDDEKAHRFPEVPGEVLRNPALERISTQYSRLRQENSRAGSTYATSVASTSGVEGSPTPEVSQGSPRPESTPIRTPARRATLEVPTPVHTHRRSESH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3I7</accession>
    <name>A0A9W8Y3I7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GDP-mannose transporter</fullName>
            <shortName evidence="7">GMT</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">VRG4</name>
        <name evidence="9" type="ORF">N0V83_007589</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Involved in the import of GDP-mannose from the cytoplasm into the Golgi lumen.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Homooligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Golgi apparatus membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cytoplasmic vesicle membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TPT transporter family. SLC35D subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3I7"/>
    <dbReference type="OrthoDB" id="417037at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00803">
        <property type="entry name" value="nst"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="207"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="361"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4367059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="385" mass="41860" checksum="31D8888F693D286A" modified="2023-11-08" version="1">MADDKKSDDYRIDMPAGKEFRAPSPLSRAPSVHRNGSNPITENAIAAILAYCGSSILMTVTNKYVLSGVDFNLNFFLLCVQSVVCVAAISILKAAGIITYRDFNTDEAKKWFPISLLLIGMIYTSTWALKFLSIPVYTIFKNLTTILIAYGEVLWFGGAVTPMALLSFGLMVLSSVIAAWADVQHALTSMGTATDAGADAMSTLHAGYLWMMFNCLCTATYLLCMRKRIKMTNFKDFDTMYYNNLLTIPILLIASILVEDWSSANIQKNFPPEQATTVVVVMIISGLSTVFISYTSAWAVRVTSSTTYSMVGALNKLPVALSGLIFFDAPVTFGSVTAIFVGFVSGLVYALAKVRQNASKPKNVLPTSSIPVSASSQSMRDSLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y440</accession>
    <name>A0A9W8Y440_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y440"/>
    <dbReference type="OrthoDB" id="5337308at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040981301" evidence="1">
        <location>
            <begin position="18"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="436" mass="48649" checksum="990A942762ACAE89" modified="2023-11-08" version="1">MLVWTFVTATSLGTLVASGILRSPSSSSIKFSQRLAREADSHDGVSNVVDELKSLALVPEVAGLDFDPKNYADAAMWELYADVKGEHLVCLMQATDKGAGFLIEDKRTPPSAASPWTGDLKNELATWFWHETDWDKSWECDWERIGLGKAFAALGLNAKPAYEDDGSPADGHNDCVSITHYDKEDYEDPEALWPMMKDVMDQKYYVGGKEYMATGGYYQFSVNKIDGAIIAININSPGSMANEFWVRRNWGRKAAPEELPGLRFCSDIYWAYWARDNPNVKSLRIYGASMIVNEQTVPLVARAFKNVGVKSLAPWPGTSFSWDTEEGIALIGSPIGATIAHMLIRHKAELGIKHISKVNVVTNDYGGGRPRFGEEPRKDLHLFFEIEDVPADKVTTDPAEQQKRDSVKHGIEVFESKVVHMNKNGSNIVREHIMRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y449</accession>
    <name>A0A9W8Y449_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y449"/>
    <dbReference type="OrthoDB" id="1022638at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843:SF2">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="38"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="241" mass="27649" checksum="CF09DF620411415E" modified="2023-11-08" version="1">MSSGHSSTDVAEQTDVTPVEFKSDYGRVLQTEAINMIDMLTVTVGTGAETVKFPVHEALLRKSSLFFDNAMKPEWAASKLDPRDVDLTDEDPEVFKVYLHWLYMRTLPTVHRDLNLRGQKHEFDQLSNCYILGEKLMDVRFKNAILDGVVDAMNNTPFCTGKMPGEASINIIYSGTSEGSPARRLLVDIWVMYAGENWIKHLTDKLPHEFVLEFSRALLKDKCNGKKDTRRWRTRMEDYHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4I6</accession>
    <name>A0A9W8Y4I6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ankyrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_007942</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4I6"/>
    <dbReference type="OrthoDB" id="195446at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="92"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="126"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="160"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="293"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="366"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="467"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="824"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="857"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="890"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="761"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="940"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="770"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="940"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="973"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1037"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1057"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1078"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1095"/>
            <end position="1104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4366306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1194" mass="130503" checksum="6E48D1616E29A5D5" modified="2023-11-08" version="1">MESPVPFRHVSLESDQHTTKPTGLKRKGSTLQTSVEVFRCRFGKPTPIPEGEGSFEPSKEWGPIYYAVYHQREAALAHFLRNGGSPDDVNGTGQPPLCIAVANGYFEIAKILLEAGADVNATTKTGGETALHIAIKNGRSDFIDTILPYGPNLEAQTETTGETPLHYAASKSGSLATVVALLKLGAKYDTLNAKGQTPAEAALQANNLNGAVAIINAAHGKRNKLLKEKEMLLKHVQKTKGRFSIGNDLIADIFSAACDPDSTVLVEAIKRDDASLVGMFLEKGSDPDRQTAKGLRPIFVALECAGAAVVQALVKHNADVSIRDARGLTVLQAAFESPMAQDKEAMSAIFDAFLAKDANAMVTYPDGKTLLHRAVSARFGHTRVAHLLISSGVKINAQDNDGNTALHLATHSRACIELLLKNGANPRQMNLDGLTPLLYATTYSDKTKEPDLSSLIKASDLRKTNPEEQTVLHLAAANGLEKTIRLLLAARAETTIVDSNKHTPLLLAVKNHQWAVVPLLTIPPNINSWDEDGMSALHHIARSIPKAPSTWQDVAAAAVPFCDRGVSRSMRDRSGATPLIQAVRTLPEDGLPVIEVLLMQKADRRASWNCVSHEDHQRHDAFFYAITLGKTAFVEALLKHGAMFTFAEWTQSKGKLDSSIAKDRQIMKLLAQYEWARRAGSLRRQPSDSDAETPVFSTMFPTKDIKQMISMGLEVNTLPKSPLGTSMLWAVLRHIPLQPPMSPKYLFDTVKLILDAGADANAGTARGSRRSPSPSSPQASRESLPLSLHPLTFLLEESPTVDIDLVTLLLTKGTKLSVASPFYGGRYPLHSAVKANRMDIVDEFLLQRADVNCEDQNGATPLFTAAEKALWEIVDALLRRGAKVEIKDNEKNTPLHAAAIGGSKRVVATLLRGGAKANIKNQKGLTPLACVPEDLDEKEKDKIVPMLKDSEEKERREEDRRRKQDEQNAAYEARMKQRKDEEMKMKLRREQEEKERLRQEQDAQTKLRKEREERQNVEALTPPTLVATKAPKKSLFKKPSLFSSQLKSSPPPVASMPKQTPMVSMRITTPISAQEQQKATLTPKPTATPRSVSTPLPPATPQPPATARSATTDFYNIPVGRSSVPAVPALPSDKPLPAPRIDSGFGQKRPSDTEKPLPPLDRNKSMLGANETAKRASGHELNDWLALSKMIDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4U4</accession>
    <name>A0A9W8Y4U4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_007079</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4U4"/>
    <dbReference type="OrthoDB" id="294730at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000039">
        <property type="entry name" value="Neutral amino acid transporter (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF20">
        <property type="entry name" value="AMINO ACID TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="383"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="413"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="455"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="75"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4367496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="503" mass="55490" checksum="558359E28A96FDE3" modified="2023-11-08" version="1">MALGIFGGNKAAEAEADKHSSSSDEKVSHPEKSYDAEAGLGRIEDPSRRDVDEADSITIGKQIEMEAENAIKYRTCSWPKTAALLFAEYICLAIMSFPWSYSVLGLVPGLILTVVIAALVLYTSMITWEFCLRHPEIRDVCDLGQMIFWNKKWAFYFTAVMFILNNTFIMGLHCLVGAKWWNTITGHGACTIAFSAITALISFCASLPRTFSTLAKLATFSALFTFISVMLAAIFSGIEKKPALYPTLGEPTVLAVPASGTTFVLGMSAFMNISYTFIGQITLPSFIAEMKNPKDFPKALWVVTICEVLVFSLVGGVVYGYTGTQYMTAPAFGSLGNEVYKKVAFTFMVPTIIFLGVLYASVSARFVFFRIFAGTHHMGNHTVVGWAAWAGILAVLWVFAFIIAEVIPFFSDLLSLMSSLFDSFFGFIFWGVAYFRMRKAKYGPDYWKTRGLRGYLGFFFNILLCMIGVLFLTAGTYATVQSIIDGYKLASFGGPFTCDNNGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y529</accession>
    <name>A0A9W8Y529_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aldose 1-epimerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008427</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldose epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y529"/>
    <dbReference type="OrthoDB" id="274691at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004034">
        <property type="term" value="F:aldose 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09019">
        <property type="entry name" value="galactose_mutarotase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.10:FF:000015">
        <property type="entry name" value="Aldose 1-epimerase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018052">
        <property type="entry name" value="Ald1_epimerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047215">
        <property type="entry name" value="Galactose_mutarotase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091">
        <property type="entry name" value="ALDOSE-1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091:SF0">
        <property type="entry name" value="GALACTOSE MUTAROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00545">
        <property type="entry name" value="ALDOSE_1_EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="36793" checksum="9EB492A4E231D3EB" modified="2023-11-08" version="1">MSSAKAFTFLPLGAIIQKFTVNGKSIVQGFPTAELYKQYNSPFFGETIGRIANRVSNAKINDLNGKSYQLPVNNGPNSLHGGNLGWGKREFEGPSTVDRDGKEATFFKYLSTNGEEGYPGTVEVRVWYVQEKEQVDGAQQEVLHIEYEAELVGDEVDETAINMTNHSYFNLTGGPTIAGTEVSLITNKYQVVDDGGIPTGPIEEYPGVNPQKTFTLGEKEPDIDDCFVVNTDPKSIPIDTRSSRLNKVASFYHPESKIHLEISTTEPAFQFYTGKYIDVPAAGDVPARGARSGFCIEPSRYVNAINVPEYRSTMVLKKGEKYGSKTVYRGWHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y583</accession>
    <name>A0A9W8Y583_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enolase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007472</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y583"/>
    <dbReference type="OrthoDB" id="2579025at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase C-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="88" mass="9746" checksum="4613F749D7D077AD" modified="2023-11-08" version="1">MAEVDAVSANFAIQEMSLGIHYHTGAEDLRSYVKNPEVWDVKDGTIDLPVGPGLGIEVDEEWVRRLSLDTEAWVSPGFFGPGGEVREW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6E4</accession>
    <name>A0A9W8Y6E4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycoside hydrolase family 5 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005944</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6E4"/>
    <dbReference type="OrthoDB" id="428177at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031511">
        <property type="term" value="C:Mis6-Sim4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018565">
        <property type="entry name" value="Nkp2/Cnl2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28064">
        <property type="entry name" value="INNER KINETOCHORE SUBUNIT NKP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28064:SF1">
        <property type="entry name" value="INNER KINETOCHORE SUBUNIT NKP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09447">
        <property type="entry name" value="Cnl2_NKP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="460"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="57"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="119"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="493"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="526" mass="58966" checksum="7EA1C39E1B6951CD" modified="2023-11-08" version="1">MPSQEAKILGDFLLAPAALRDFVTLRQFTDIFPKSHRANPAVQDLYRELQRLREKDIEVVRRNIAEEVKRSKQLRREYAKERRQLDGATVAGLDIIALQMEEEIFGEGHTRPHSLHTVHASIEEACQSLEAQIEEIEDANRTALVEVQEVVVRPDEQHDDPEGELGGFDKSLLDQFDDTMAAIHHLSKGQMKVIIAPHDAHALRGTNDVPCDAYCKKLDGAFLDFYSSDEMRELYKTRLDVFFKHYPSKNFDGRPWGDLSEIILGVDLQNQPFSGIWPIPSGESWLCDIANHLKFTVGLDKANIAVISGGISGPQKVDGIQNFPDSAFECTAIDVIGIHGYFSKEKDATAGTPWANMFVPGNTLTARAKGKEGKGKLLLVEEWAYVHSDFGLFYKKEAIFDQANALNYRGIPWLYSHLTTLNEGRTARVNPLRSDYTSWAALKEVLKRSYTTRSNFNWNKYLPPPSSGPTTQQPGAGEWNGGKKNQRIPGVPKGHERGPAKVRTEAMKINVPKEKIAETGRPMATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6Q1</accession>
    <name>A0A9W8Y6Q1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007596</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6Q1"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="190" mass="20691" checksum="55FD9ED057902F07" modified="2023-11-08" version="1">MAIKQKPDDATLSAQRAQIHKALQPYSKSLHSKYDDVKTAAGHIKTVINRLAFAKKPSFTWASAEGTLWTTTKLKHELCRRVAITLWNDGVIQLAKPPSNGDHHTAIQQQPVVESEKGIQTAPADPDSDYGGFPSLDDVIALTAHEQSSSAPNGRVLKRKDAETDDGAAPALLAERKKQKQTTLCGCRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6T0</accession>
    <name>A0A9W8Y6T0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial ATP synthase epsilon chain domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ATPase epsilon family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6T0"/>
    <dbReference type="OrthoDB" id="269124at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12153">
        <property type="entry name" value="F1-ATPase_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1620.20:FF:000003">
        <property type="entry name" value="Mitochondrial ATP synthase epsilon chain domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.20">
        <property type="entry name" value="ATP synthase, F1 complex, epsilon subunit superfamily, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006721">
        <property type="entry name" value="ATP_synth_F1_esu_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036742">
        <property type="entry name" value="ATP_synth_F1_esu_sf_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12448">
        <property type="entry name" value="ATP SYNTHASE EPSILON CHAIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12448:SF0">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT EPSILON, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04627">
        <property type="entry name" value="ATP-synt_Eps"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48690">
        <property type="entry name" value="Epsilon subunit of mitochondrial F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="8396" checksum="C224F0FAA8D09901" modified="2023-11-08" version="1">MAFAWKAAGLTYNRYIAVASRVVRRSLKEDKRIAAERRGESELRFAKWENGKQGEVKDLNAAALQEQANASKPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7M4</accession>
    <name>A0A9W8Y7M4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribosomal RNA assembly protein krr1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">KRR1_2</name>
        <name evidence="2" type="ORF">N0V83_007184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7M4"/>
    <dbReference type="OrthoDB" id="265717at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053185">
        <property type="entry name" value="SET_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332:SF6">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="132" mass="14833" checksum="913F0DA4C4595613" modified="2023-11-08" version="1">MDEDPSALAAQHINTDPGTWQATPIPGKGIGMLASKPLNFKDRVTAYTPAFLAYLETELSTLDREAWWKLAIEQLPEKTKADFMNLTYVFGDMRIRIQDIVKANTFQVDVEGVNHLAIFPETSRLNHACNPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y896</accession>
    <name>A0A9W8Y896_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein csh3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CSH3</name>
        <name evidence="3" type="ORF">N0V83_005069</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y896"/>
    <dbReference type="OrthoDB" id="5229808at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013248">
        <property type="entry name" value="Psh3/Shr3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28228">
        <property type="entry name" value="SECRETORY COMPONENT PROTEIN SHR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28228:SF1">
        <property type="entry name" value="SECRETORY COMPONENT PROTEIN SHR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08229">
        <property type="entry name" value="SHR3_chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029187">
        <property type="entry name" value="Shr3_AAP_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00786">
        <property type="entry name" value="SHR3_chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="201" mass="22334" checksum="6350240BF204AD01" modified="2023-11-08" version="1">MSGNNFAQFMVVGPTCFFLGVLFAQLPYDYNVLWTMPEDRTAYFNILEDHIRFLYASPPLISRVLNLAIGTGLLGFLIKLFRPNQANMLFDGASLVLYMAGITVYLTNIVKGFRVITAGIYGDAAKAALEGEVLEVDEGDFIGREDTLRVFAASNTILALVLVGVLVLQAGMWYAKRAEEKEMAEFKRKDDEKKGAGKKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8G0</accession>
    <name>A0A9W8Y8G0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">A-kinase anchor protein 7-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005865</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8G0"/>
    <dbReference type="OrthoDB" id="277832at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019510">
        <property type="entry name" value="AKAP7-like_phosphoesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009210">
        <property type="entry name" value="ASCC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13360">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13360:SF1">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10469">
        <property type="entry name" value="AKAP7_NLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="A-kinase anchor protein 7-like phosphoesterase" evidence="2">
        <location>
            <begin position="69"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="246"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="324" mass="35563" checksum="171CBFFE9CADED2C" modified="2023-11-08" version="1">MGKKKANGEYNDFLDGEKLRDNAGVRTSLQTTSSATKEISPPPVRQSQDQRGGKSKGKGKGGPKKPPLTHFLCLPLVTDTSRPQLQSGLDKLKDELTKENVIPPKAVRPVGTLHLTLGVMSLDQTKLEEAKQYLQDLNLHSLLRDISMQKLAEQAAEDGTIAENFCSGVQMPDSDALTINLESLVPMQAPHKTSILYAEPRDKTQRLGVFAEEVRSSADLEPTQHNGDAEAGAEDTHSQDTNEEQASSAAGSSSSHGPEAKSWLRFDARHLIDSYKHFVWADEVRVDRVQICKMGAQKIYSEDSDGVVVDERYEVVAAKGIDDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-06-18" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8J2</accession>
    <name>A0A9W8Y8J2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005133</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10256089at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031991">
        <property type="term" value="P:regulation of actomyosin contractile ring contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032955">
        <property type="term" value="P:regulation of division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07589">
        <property type="entry name" value="BAR_DNMBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000053">
        <property type="entry name" value="Rho guanyl nucleotide exchange factor, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051492">
        <property type="entry name" value="Dynamin-Rho_GEF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22834">
        <property type="entry name" value="NUCLEAR FUSION PROTEIN FUS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22834:SF20">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="2">
        <location>
            <begin position="954"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="470"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="523"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="729"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="839"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="917"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1026"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1453"/>
            <end position="1567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1596"/>
            <end position="1645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="202"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="317"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="419"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="470"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="537"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="739"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="853"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1041"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1599"/>
            <end position="1630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1730" mass="190916" checksum="FABF4E653DCEF1C7" modified="2023-11-08" version="1">MAYHPNIANWIAQLDSEQKHADDSLETSPEDYYTSAYPSTEHDAPPPLEMAATRQRHPTSNGVVRPPPKSTPRSASGPASSAAATRSSPQVPNKVKALAERFERSPGSSPATTSPAVRSRSREPSRFGAHDPAKRSNASVSSTASAARPSKEAAYGSYKFNHLKPRERPQPAPAAPRTARRTNGSRSSIDQQISPTRKKVTSPSRRPPSSGRQPFFGEVVSGQDVTSPGFGIPTFENTLGDEATPDSVHSVARTHDPLTDGPRLPATSFSSPQHQRSASGATSSYAGDGQQSQPVDHTTTQSPQWRTPRSKIPVATRRLSATSDSNSSTRSVKYTPTRASNHSNRASPTRLNRKAAGPTGTPKPNGSDQATLPAMAYRGYRERGKSPMGSGGGPSVPAVVTAPLAPTSPRLRNSRERQSLQPSPTSRSRSTGPHGEQGYFPTGGTFDPQQNSLSHDEVPVAVDHVPKVELSSDTHEPHQQPEHLPQEENHYSSSHHHQRLSLQTSSLSLPPIAQPLSATTDFEESPVLGIPGSFVLTPPTTHQEPEQPLLQPTVFHQPTRDQAPASPDVEQSELGVRESIPIMLGEERSANSWEPAFDELSEGLQALSFGPPSSNHAYDHRETQGVSRYDFQDSPIDHFPNRETLRPDDSASIAPYRTFESLAFNNETYSVVNSVLNMYHQSQVISPELATVSRERVQQVSPVIAQHKDWASKEATETYLARLLSDANASDNEVGREQPGQTTSSSRPSLDVQPISQDSPDVEVGGTAIIYPSESRRYSRGSGGSTTTTIQDDASRSGSSSGDPMHDSLLRGSTMDHAPASASNGLELPQLAWTNGGLGLSLQNASHHSPPSQQAPPRPAYSPPPPPTKPSIDHHVNKSSTAALHIQPERPSLSDEQFAMHPARSRTPLIQTHEKSLEDRYGTPQPVEISNKVPAGAEKSETDQPDPAKQALIRRYRILEELLTTEHQFCIDMMIAHNIFEATAQNLMSEKDKKLLFSNCKELEKFSLSLFRSFKKAAKPIVNYEIPPPTGPWNRDPSDTRPFNPSDTSLNLRRPSEDSENPFENCTLENDRLTTVGRVFLENREKMERLYTTYFLNQDNASAFIKRNQGNPELATWVIACFEQVENMTQAWDLDSLLLKPCQRLLKYPLLLQGLEEACDPEHPDLPNIRQARKELLAINARINEAKARSDTFRAAASEGKKEKKKGTGLGKTFVKAFIPKVDKSKAYDEADKTFKDQEYKSREQKFGGHFFQLQIVIRDFEQYLESITEHYLQLNIVFLGFITVCENKALEDHKSAVRARVLKPVGDLWEMWVSYQKTIEQRKKFLLYYVKHKQAVDKGEKIDPDLEESAKKFTTINDTLKHELPLLYERTKGLMRTLMTLFMCLQKDWYKTCSKKILPLLESEPQHTTSLRYDLESYVERFHSDYKPMQDLANRLGIINRNLLIDISNMTSPVPTLSSDGGGSSRNSSSRRTESMGSEASILDARNRNSGGYGSRPHVRTFDNPPRSSPAGPAYPSLFQGGAAGRNGPGPTSLREARALSPVSDNSEATVIHSERRNTGPSRNHNYLGLDGAVEYDDRSPLGSSNFDFPPQLGPSFLSPTSQPMQFSISQPTSQSTSLPGSSRTSGIFSSALPMSDSPIRGSVEELPTTPADTDEPEVLFLAASLFEFNIAHDRREGGIPYLVYVPGEIFDVIGMKGELWLARNQDDPTKTVGWIWEKHFARILPEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9S0</accession>
    <name>A0A9W8Y9S0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003904</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9S0"/>
    <dbReference type="OrthoDB" id="332863at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070290">
        <property type="term" value="F:N-acylphosphatidylethanolamine-specific phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070291">
        <property type="term" value="P:N-acylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070292">
        <property type="term" value="P:N-acylphosphatidylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024884">
        <property type="entry name" value="NAPE-PLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15032">
        <property type="entry name" value="N-ACYL-PHOSPHATIDYLETHANOLAMINE-HYDROLYZING PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15032:SF4">
        <property type="entry name" value="N-ACYL-PHOSPHATIDYLETHANOLAMINE-HYDROLYZING PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038896">
        <property type="entry name" value="NAPE-PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="2">
        <location>
            <begin position="93"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>an N-acyl-1,2-diacyl-sn-glycero-3-phosphoethanolamine</name>
            <dbReference type="ChEBI" id="CHEBI:62537"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>an N-acyl-1,2-diacyl-sn-glycero-3-phosphoethanolamine</name>
            <dbReference type="ChEBI" id="CHEBI:62537"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR038896-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="347" mass="37816" checksum="E3F9DBA353B696F7" modified="2023-11-08" version="1">MRTRQPTISAAPPTGFKNPWPSYTSSGLFSAFRTRINTPKNFVPVPADRLGLVTVHEPDFARAGQNGLKATWIGHASFLVETTAVEGSPRGMRMLLDPVWSNRVGPYGMVGPVRFTPPPCTVDELPHIDAVCISHDHYDHLDSGTLQKLMSKQNGDLRFFCGLGVKAVLTGLGAGITAEQITELDWWDGVTISDKHAGSVDLIFGSSKSHCLPARNSFFAGDTGYCHVTYDTQFSHHDASHPPCPAFKQIGHMYGPFDLALLPIGCFKPRSLLSGQHASPEDSLSIHNDVRSKKSIGMHYGTFRGGFSASYEPVTEPPKRWKEGVVAANLEWGIDVLLCDIGETVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA06</accession>
    <name>A0A9W8YA06_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_004628</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DAMOX/DASOX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA06"/>
    <dbReference type="OrthoDB" id="409956at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003884">
        <property type="term" value="F:D-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006181">
        <property type="entry name" value="D-amino_acid_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023209">
        <property type="entry name" value="DAO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530">
        <property type="entry name" value="D-AMINO ACID OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530:SF30">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000189">
        <property type="entry name" value="D-aa_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00677">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="7">
        <location>
            <begin position="46"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000189-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4371411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="272" mass="30792" checksum="05F286EA173B8D78" modified="2023-11-08" version="1">MQLDSFAVLWQLAVSNPESSIRRIEMNDFHDDKTIDQIWYRDALPEFRVMKPEELPKGTKLGMSYKTVVLTPKIFLPWLRRRLEESGVIFKRMNLSSLSEAKSQGQDVLVNATGYGSKFLEDIQDQDVQLVRGQTLHVKSNYDKIYMRHGKDYTYAISRLDGTCILGGIKQYGQTEKEVDMDIQQDIIRRVHTDLPDVFPAALKDYDVIGHNVGIRPARATGLRVGKEVMAGEKVVHAYGVAGGGYVFSFGVARAARDLVDEYVYQSPLAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA46</accession>
    <name>A0A9W8YA46_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Erv26 super protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">SVP26_1</name>
        <name evidence="1" type="ORF">N0V83_005130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA46"/>
    <dbReference type="OrthoDB" id="3796796at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4370609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="192" mass="22590" checksum="1B03AEBBA3E6CD8E" modified="2023-11-08" version="1">MITASGVMWHALVPTKFKSLDLCFHPQVVDQFVYYCYLADYQFDQDNEAPEIHFATSFPPDKDINSFSYRKSAVDFHLEMYELAEHVESEFLKETVHQKLSVVILARQKYTALELGMLVEIFFAAVDAARLQDDDRRIENIFVTAVLINERVRWNRDDSKVFWNMIGGYRREFVEAYREAEKANRDLLGSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA55</accession>
    <name>A0A9W8YA55_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial</fullName>
            <ecNumber evidence="4">2.1.1.201</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ubiquinone biosynthesis methyltransferase COQ5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">COQ5</name>
        <name evidence="6" type="ORF">N0V83_004837</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Methyltransferase required for the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol + S-adenosyl-L-methionine = a 5-methoxy-2-methyl-3-(all-trans-polyprenyl)benzene-1,4-diol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28286"/>
            <dbReference type="Rhea" id="RHEA-COMP:10858"/>
            <dbReference type="Rhea" id="RHEA-COMP:10859"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:84166"/>
            <dbReference type="ChEBI" id="CHEBI:84167"/>
            <dbReference type="EC" id="2.1.1.201"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion inner membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. MenG/UbiE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.201" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA55"/>
    <dbReference type="OrthoDB" id="6329284at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008425">
        <property type="term" value="F:2-methoxy-6-polyprenyl-1,4-benzoquinol methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000133">
        <property type="entry name" value="2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01813">
        <property type="entry name" value="MenG_UbiE_methyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004033">
        <property type="entry name" value="UbiE/COQ5_MeTrFase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023576">
        <property type="entry name" value="UbiE/COQ5_MeTrFase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01934">
        <property type="entry name" value="MenG_MenH_UbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01209">
        <property type="entry name" value="Ubie_methyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51608">
        <property type="entry name" value="SAM_MT_UBIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01183">
        <property type="entry name" value="UBIE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01184">
        <property type="entry name" value="UBIE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 4 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="188"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4371617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="320" mass="35431" checksum="992739210014F19E" modified="2023-11-08" version="1">MQSSRTRIGRQLLEGSKRRVVREPYRCFSCSVRQAQQQTPPPKSPQDGRPSTTHFGFETIAEALKEQRVGAVFSSVASSYDSMNDFMSLGIHRLWKDHFVRNLNPGRNPLTASSSSPASEQGWNILDIAGGTGDIAFRMLDHASTINNDAHTKVTVADINPDMLAEGKKRSLQTPYARSPRLKFVEANAENLDMIPDNSIDLYTVAFGIRNFTHKEVALKEAFRVLKPGGVFACLEFSKVNNPLFDAVYQRWSFAAIPLIGQLVAGDRDSYQYLVESIAKFPSQTEFSNMIKDAGFLVPGKGYEDLTNGIAAIHKGVKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA60</accession>
    <name>A0A9W8YA60_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005667</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA60"/>
    <dbReference type="OrthoDB" id="3790458at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.150">
        <property type="entry name" value="Haem-degrading domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052517">
        <property type="entry name" value="GlcG_carb_metab_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005624">
        <property type="entry name" value="PduO/GlcC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038084">
        <property type="entry name" value="PduO/GlcC-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34309:SF1">
        <property type="entry name" value="PROTEIN GLCG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34309">
        <property type="entry name" value="SLR1406 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03928">
        <property type="entry name" value="HbpS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143744">
        <property type="entry name" value="GlcG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="196" mass="20478" checksum="81E61F4C9662EE9A" modified="2023-11-08" version="1">MDGAKITSINTAMDMAFTAAGNCIPTSAYQEHSWPGSATSGIGNNISGRFTTLGGGVPIISSTGEILGAIGCSTGTSMQDEEAAKAGRDAVLALTRKEREDEDRRINEERAAVLKLWKEKEEEVGNLREELVGLERGSKRIRLDGGRSGSVSVSESVLGRRGSAVGSAIGGAAPDTPPEEGQLHPSFVGEVTVGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAB9</accession>
    <name>A0A9W8YAB9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tail specific protease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_005564</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAB9"/>
    <dbReference type="OrthoDB" id="27214at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056186">
        <property type="entry name" value="PDZ_CPAF-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052766">
        <property type="entry name" value="S41A_metabolite_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005151">
        <property type="entry name" value="Tail-specific_protease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37049">
        <property type="entry name" value="PEPTIDASE S41 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37049:SF4">
        <property type="entry name" value="RHODANESE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23658">
        <property type="entry name" value="PDZ_CPAF_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03572">
        <property type="entry name" value="Peptidase_S41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040852081" description="Tail specific protease domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="CPAF-like PDZ" evidence="3">
        <location>
            <begin position="167"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Tail specific protease" evidence="2">
        <location>
            <begin position="367"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4369800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="756" mass="82269" checksum="0A4F0DA004A09DD1" modified="2023-11-08" version="1">MKTYTFLALAGLTTLATATPISPSRTQPLQARQGNGTATAPCAKVSQYMYGDGTTVVKQQIPADIAWACLSDVPFNASSAKALIKSIRPYMDWQSTIDVLKNPPAEYVEKVQPAIDIIGGLDKIDADIDAGSFKNEYDFGWNLYTLIQSAHDGHFAYIPDSVGAIFTWGRPVPLVSVSEDGSKLPAVFTFDDVLGMQFKNISYTPSAVVKIDGTEITEYMENLSQYGSLQDRDALYNNLFYELAQVSLGISGSGTGMFSGGGRGRYVYPGVSTTLKFANGSEYTMQNYARTTISFRNMATGEDLAAKWFSWGTGEAQAQAESETPETISIADTDAAGAAAPGYPAPVVAGPQNLINGFYVDAPGYEDVAVLQVPNFVGSTAAEVPFQRLTRDFIPKALADGKTKLIIDLQANGGGTILQGYDMFKQLFPELDPYGANRYRATEAADLIGQSYSAYASQFERVSSVNSTVLQAQASYFDYHNDMTVDEKPFSSWEEKFGPQEANGDKYTTLNRWNLSDVHISYSSGGINITGYGPLANVTGPAKFKPENIVIVTDGYCASTCTIFSELMTQQAGVKTIAMGGRSNKNKIQAIGGVKGVNNYQFGYIQQLAQYAIRYTPSLNTSILKTDYYSDLPYNRATISPGVNVRDGLRQNDTSGVALQFKYEEANCRLYYTPEMTVDITSLWKAAADAQWGNSGKCVSGGNYGQKREAHEVTANLRPRRIQVSQATALKKVQAFENSFSLETECKMTGDGFMHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAU3</accession>
    <name>A0A9W8YAU3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_004154</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAU3"/>
    <dbReference type="OrthoDB" id="269227at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF115">
        <property type="entry name" value="DEHYDROGENASE XPTC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040929921" description="Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein" evidence="4">
        <location>
            <begin position="20"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="5">
        <location>
            <begin position="283"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="537"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="580"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4372380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="601" mass="65271" checksum="307EF7732F0FD490" modified="2023-11-08" version="1">MRLHVLAFVIPSLLRLGTAATVERKSTNPAASDAYDFIVVGGGTAGLAVASRISLGLPHRSVLVIEAGPDGRQEPGISVPGRKGSTLGGRYDWNLTTVPQPHANNRTLPQNRGKVLGGSSALNLMTWDRTSIAELDAWESLGNPGWNWRSLYTSMLNVETFLPSPEYGTEGVGRSGPIKTLINRIFPRHQATWIPTFNNLGLATNRESLNGRPIGVSTQPTNVSPNYTRSYAPEYLGLAKKNLILMIDTRVVKVNFKGKTAVGVTLENGTVVRARREVILSAGSFQTPGLLELSGIGNASILKKFNIPLVKNLPSVGENLQDHIRIQSTYQLKPEYPSFDVLRNTTRAAAELALYNADQVSLYDYTASGYAYFPWNIVSNDTAAQLHALIEGDKSLTSAVDKIKKSYWSPALSKTVPQLEVIFSDGYTGLKGYPAANSSLFGIGTFALIGVVQHPLSKGNVHISSRNISDKPVINPNYLSHPYDIQAATSLAKFLRKIATSKPMNDVWTMEYEPGAAVQTDEDWRRFTLANTLSIYHPIGTAALLPEKDGGVVDPCLRVYGTKGLRVVDASIIPLLPSAHLQTLVYGIAEKAAKIIIEGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB88</accession>
    <name>A0A9W8YB88_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pre-rRNA-processing protein PNO1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Pre-rRNA-processing protein pno1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PNO1</name>
        <name evidence="11" type="ORF">N0V83_005089</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for small ribosomal subunit (SSU) synthesis. Has a role in the processing of early nucleolar and late cytoplasmic pre-RNA species.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the small ribosomal subunit, ribosomal RNA processing complex (SSU RRP complex).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PNO1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB88"/>
    <dbReference type="OrthoDB" id="1932641at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22391">
        <property type="entry name" value="KH-I_PNO1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22392">
        <property type="entry name" value="KH-I_PNO1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000009">
        <property type="entry name" value="RNA-binding protein PNO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055212">
        <property type="entry name" value="KH-I_PNO1_first"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055211">
        <property type="entry name" value="KH_PNO1_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826">
        <property type="entry name" value="RIBONUCLEASE Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF13">
        <property type="entry name" value="RNA-BINDING PROTEIN PNO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22891">
        <property type="entry name" value="KH_PNO1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="10">
        <location>
            <begin position="164"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="42"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4370568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="259" mass="28606" checksum="323BD2CC374C073B" modified="2023-11-08" version="1">MPAPTALMRKPEELAEGVALEATDVPLPIEDDAELIDMNFEPTGAPDETLAASTEDTPMTDETGRPKFGPGKAIPLAFRREQRKISIPPHRLSPLKSAWPKIYPPLVEHLKLQVRMNTKTRSVELRTSSTTTDTGALQKGEDFVKAFTLGFDVDDAIALLRLDDLYIETFEIKDVKTLNGEHLGRAIGRIAGKDGKTKFAIENASRTRVVLADQKIHILGGFKNIHIAREAIVSLILGQNPSKVYGNLRTVAGRMKERF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBF1</accession>
    <name>A0A9W8YBF1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003278</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBF1"/>
    <dbReference type="OrthoDB" id="3882589at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="315" mass="36116" checksum="865454D0656CB47D" modified="2023-11-08" version="1">MPVLAQGKTMGNGTSSQRVTHAAFVKREDGEVRQFAYCYDAQLLAYLAERSISPTFLHEIDSFFLDHPELASYEKGRKFLSDMRMLSPLSAYITFIIGYTMPARYIYDINTKLPLLLQLPPGFSHTPLSEKYEYDPELMDIFEDWGPSWGHAARLRILDQIDETVSMGIRKLALGRPVNYDLRKLICYAIWGNPVDAYKWFRYSYQLDNLKDHYLPEHIILVQAPNFRGYAEPQPLVPGIDVLDVLVKDYRIALVRSGHSRVEIEKRCATAKAKLAQAPTGIDIVDLIMDDAVGRRGTNRRGARKPRNTTRTKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBK6</accession>
    <name>A0A9W8YBK6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003772</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4371999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBK6"/>
    <dbReference type="OrthoDB" id="3800826at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="549"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="602"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="762"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="556"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="621"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="660"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="681"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="769"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="782"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="798" mass="90624" checksum="17EB6905B9E79B91" modified="2023-11-08" version="1">MFAHADQENVAAYLLYLYGYHSTYRKGNTARAAPAKTKLFDAAESTPRGVSRSDAFPSLPPEILQSIFSYKKVDVQKLQAYITISSNPQPLVEAVRTREIDLTSKLFHLKKKARLFLRNTSRHLDMVGRIQTVSKLIDTIRRARQILDPGFSRSDSVFSNSSTHSPQSVDAQRRPPNRFTGVSYTTLQEEIKAVQQWRAAQTRITNLFQQQYIVALRQKGRESSLSLKRKLETALLKQQISMSYLKALYDEASSNLISGNQAKEESLLDDATSFANKLIPLEPLYAPYNVHHYVLGNIVDQFKEAMYKFGRREFPVIMESRGWTSAESVPVAEFMLVLRRRADTHIRDRYNFFEKEFTSLRLVRNAHAHSSDSMTIQDTRTSLADMRIAAEALRFPEMVPKINQYLKLLESFEHDYNKKKEESQERALPILRGLHENRESAIKKLEPRIYGTQEQERILESQKNAIREFFYHKISSFLRQYKTIDRHTAAESAQELAAFALEEHIRRSLRAMSGPAAKSLVQRLFAEHTASETVTADASVIDPVQISEASSAMPGASSSETSNNNPAIATPPSGNDEDQGIGQELSTHMSSTQLDELELDRNEVTSIWYPPSRATRTQGKPTSSRASSTRPPKGYKKVDPAPFVPRTTSKPPWTSERHTAHSAKIKARKEKLRRRERHDTSAPPSSTSETHPPTSSTKLTAQKKERSRHRERHDDASSPPRPFKAPRGPRPSEEQLEGFIKHWNDTEAARLDREMTNWPAKRVVFPQENSKEERVGKREWVGDGGGVGRGIQSGSFFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBU6</accession>
    <name>A0A9W8YBU6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002604</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBU6"/>
    <dbReference type="OrthoDB" id="10251048at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="685" mass="75056" checksum="B7D2CAA8FDD97403" modified="2023-11-08" version="1">MAGRFHEKEDDLEGQQPTPGPVSSTGIIKLQPNRKNIDRTWRPVPASDLEKYSTAARDARCDSSTAGRLLLSNTTITSLMDSRYDLSTEAFPILESKELSENHTDRSGDPIETSRASNNEYCPNFSRSFAGDHYGQLFGKLPDPICLHEEMGEFDGQVIFIGHPNRDVSAHQWSSASFQWVNIGRYAHSRSKVEGSLASDRLKENCVSHDPLAFFKSAAENREKLIVENGRTKEVPSGALQSAPANGARTASLSTASDPIVEKFPGLIHNNVPSNPIHLEDDDPLTSMTLQASVGMECLEDPFVAPTESSTLSVGPSLKIGNDRNDPKGSLDLGYAFPLKPGISAVSDHSIRSTSQNLILEGKRNHHEIFTGLAHRQLETALRDINLGEDAAGGHALSSGRSFLSRRTPLPASSENTWDNRTTRDRLAELSDLTLQPSGPGQEGIALPTYTYVQPEARSLHHPPGLTVANPHRIVSSLNASAPTYANMRRLTPGPKSSAFEAMNFNATAATLRFSDPDGLRQSQEYEIANGLGHQAPTVQCFKGPFFTDSKPTTSDPTVSLSVPIGEEEKLYNWFRDGHRPSRQQEYAKTLISAAAPSNRCRSLGAVGEAFGGPRDRYYENTSPFIRLYESLSEYLEEYSNGSGRSYFTRAWKPAAPRLRDLDQDGNNSYFHGPTDILADPKAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBX0</accession>
    <name>A0A9W8YBX0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">U3 small nucleolar RNA-associated protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">UTP10_1</name>
        <name evidence="3" type="ORF">N0V83_003710</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the ribosomal small subunit (SSU) processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HEATR1/UTP10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBX0"/>
    <dbReference type="OrthoDB" id="31183at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034455">
        <property type="term" value="C:t-UTP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040191">
        <property type="entry name" value="UTP10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13457">
        <property type="entry name" value="BAP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13457:SF1">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="1" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="165" mass="18607" checksum="0B993CA4E27872C6" modified="2023-11-08" version="1">MNRRPWLWRPPGPPAEAHAPASPASINMATALQKQLAAIAASSTHQLDLKAQKSAHGKSLLFESKVAASQSFEALYLICYDGYRHLCALDPRFLHFQKSLFSEQSKSEDRTQMTKAENEKLDHALEAFITLVGPRLLLKPAEKALEWLVRRFRYETASHGYGIYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC25</accession>
    <name>A0A9W8YC25_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004731</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC25"/>
    <dbReference type="OrthoDB" id="5413829at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2790">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="326"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="357" mass="38376" checksum="87715973C27E0B29" modified="2023-11-08" version="1">MAVSDEKTTPKSAHLSDPALAPFLQTRFDSAEYLNATLPSLSLSSVSSRPLKSGNSASLADLSSQTQDLLSQLNAHTTRLSAILTQLTDDILRSGGRLAYEVEVLRGETIGLTETLTDGAKGDIEKFLPGGISLTAKLADEEATPQAAQDEDAQLEAAPLDIDETTPEYISDLRTLTTVRTRLESVIKVFGEAMHWTIPPSDLSLSASLISVSAPEPGSDNASREQKGKEFASSLRSEIADLITGDPAGNGKEKAEVRIAALRDLAQVWKGTAEEKARGKFVEGLVKLAEDKQREIDREKERSRAGGQQSRGGRSGGVPQPPQQSQPPQLQQQRSSGGGGFLENLQRIRGFVEQEMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCE4</accession>
    <name>A0A9W8YCE4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Endoplasmic reticulum-Golgi intermediate compartment protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ERV46</name>
        <name evidence="9" type="ORF">N0V83_004208</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in transport between endoplasmic reticulum and Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ERGIC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCE4"/>
    <dbReference type="OrthoDB" id="270930at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045888">
        <property type="entry name" value="Erv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012936">
        <property type="entry name" value="Erv_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039542">
        <property type="entry name" value="Erv_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984:SF25">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07970">
        <property type="entry name" value="COPIIcoated_ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13850">
        <property type="entry name" value="ERGIC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter N-terminal" evidence="8">
        <location>
            <begin position="8"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter C-terminal" evidence="7">
        <location>
            <begin position="141"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4372434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="437" mass="48680" checksum="D24A29425E8B10E5" modified="2023-11-08" version="1">MPAKSRFTRLDAFTKTVEDARVRTTSGGIVTIVSLVVIFWLTWGEWADYRRVTVRPELVVDKGRGERMEISLNVSFPRVPCELLTLDVMDVSGELQMGVTHGINKVRLSPEAEGSRVIEVKALDLHKDEATHLAPDYCGDCFGAPAPTNAQKPGCCNTCNEVRDAYASISWSFGRGEGVEQCEREHYAEHLDAQRQEGCRLEGSIKVNKVVGNFHIAPGKSFSNGNLHVHDLENYFKDEYSHTFTHRIHHLRFGPQLSDVVVAEMQRKHLATGPGGWTSHHINPLDDTDQHTDEKAFNFMYFVKVVSTAYLPLGWEKEGSHPAKYDELIGATIDASYKGSIETHQYSVTSHKRSLQGGTDEQDGHKERIHARGGIPGVFFSYDISPMKVINREVRDKSFSGFLVGLCAVIGGTLTVAAAVDRALYEGVNRIKKIHSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCF1</accession>
    <name>A0A9W8YCF1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003759</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4371986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCF1"/>
    <dbReference type="OrthoDB" id="3796057at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="527" mass="57851" checksum="43EE2B7DA2F49F3C" modified="2023-11-08" version="1">MSPQTDSATPSPPLQPIPPLEESRHAHGNGTIRPFPFPSSSLPRHIKATASFSLADVLSEIRGERRGYPLPDPPETCAAVNETSDEPAVVDTSSNQTGVEDKSYEGEVHAHSDELDERYEDEGESTEISDTDVEDDVEDVQEQEIEISGDDGEVDIEDYEGDTDAVEDVINLVHGKIASVAKKLDLTAYYIGEDVDEKQEIEFSGDDGEADIEDYEGDTVEDVMNVPPGKIDPPAKKQDLSAYYVGDNGTDGEDVDEKKKAREMARDKIHMKLRRDLEKEASGRPTSAGDSNEYDYDALLDKVVQDGMDTLPREEDLRLIYLAHGFPAKGSEKVSLILTATGRYIINRIPLLKRDRQALEDSSEDQPAPVSAQDFIPRQYNASMTSPGSSTVDTKTTVAHTPAGKQINFAAHATVPDPFTTTLDDLKQYKGKENSPVKKRRKASSPEEIAELARKAEAIVAAEEDADVVSDQDQEQKQMILYSPEPTFITVIGMLPATVMWTTAAVVAKYSNKAYDAVIEKLTGLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCU9</accession>
    <name>A0A9W8YCU9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PhoD-like phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003985</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="9999821at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07389">
        <property type="entry name" value="MPP_PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.70">
        <property type="entry name" value="PhoD-like phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018946">
        <property type="entry name" value="PhoD-like_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038607">
        <property type="entry name" value="PhoD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043904">
        <property type="entry name" value="PhoD_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46689">
        <property type="entry name" value="MEMBRANE PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46689:SF1">
        <property type="entry name" value="PHOD-LIKE PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19050">
        <property type="entry name" value="PhoD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoD-like phosphatase" evidence="2">
        <location>
            <begin position="742"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="domain" description="PhoD-like phosphatase" evidence="2">
        <location>
            <begin position="1005"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="415"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="514"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1159"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1197"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1369"/>
            <end position="1767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="119"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1165"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1274"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1386"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1513"/>
            <end position="1525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1526"/>
            <end position="1546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1574"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1651"/>
            <end position="1665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1719"/>
            <end position="1733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1780" mass="197804" checksum="E7B666373DF39893" modified="2023-11-08" version="1">MENTRASGSSRAVNPALLKVPDAYPQKRTSYRRPVDAQPAAAPQPAPTMSNTIPSNTARPVAAAPPSNGIPRASSHRRRQSQQIPPPTTGAMSESVPAAPDVPRAPPMSYKDPYAQKASSRGPPRSSSGRSRDPAIQLNPSRQQATIQTAADRLYDLRSPPYEPIEPLAGTVERRNSQRRPSVPDRSPLQKLEGKLDDISKEERRARILEAELAAQEKAEAEMRARRARDAAERQQRVVSQPIPREKPITASNRTINTKRHVSAPMSHQSPEMSDDGFVYDISPPWDPATGQGAEAPAPHRVPSQKQPRGSSGYQQAPYDESGIPRSTSLKGKEPVGVSRGAGSFRDRSAGPTSQNVNRKPAAGPTGLGLVGVDDTSAGAEVTRSDSKRVGQGRRDSRGIMAAQMEMQQQLIDQKGMARDTDARQLQPHDPPVAPTARKSVGFSEPDANVQPGASQESHHHHHFRHHDEPERTYIAPPMLEEWKGAPVGTLTADDLDLEAPVRSNSGNKAWWEESKASRRRRSSGYAEPTYDGYTDDTPVPTTFTPQLYLKCGPLLRYTGLRRDRSRGKEREVWRGSVMIVTVDAQSSYSMPPTLRLFKQPMDILPPPPLEVDDDQLDPSYVDPIEGQIKVSRTGKTLYVKPIDELAENEDLSRIEDDTGLFATSRSSPNGSATKSTRIHKKDGEKLGKLRDIPGIRLHTERGVTFWRFNLEIELGSSQARVAYKINKGPAIGFWVPAKGESMNIMFHSCNGFSFSVESKEFCGPDPMWRDVLNTHQTRPFHVMLGGGDQIYNDAAMRQTTLFRQWTESRNPLQKHNAPFSEEMQNELEQFYLDRYSMWFSQGLFGMANSQIPMVNIWDDHDIIDGYGSYPHHFMQTPVFTGIGAVAFKYYMLFQHQSVVAETEKTEPSWVLGKSPGPYIKERSRSVFMHLGRQVAFLGLDCRTERQRDEILTEDSYDIIFDRLEDEIIKGETKHLIVLLGVPIAYPRLNFLENILTSRLMDPIKYLGRAGLLGNFVNKFDGGVEILDDLDDHWTAKHHKEERNWFVRELQHIASTKSVRITILGGDVHLAAVGQFYTNKKLNVPKDKDHRYMPNVVSSAIVNTPPPDMMADIINKRNKVHHLDPYTDEDMIPIFTHDVDGKKRNNNHLLPHRNWCSIREYKPGTTPPGTPSPPTTPEDGPQVARTLSDYAPAQMVRRLSGNQTRPSARSRGPPLSYYNNPAYAAADEEQNGPHNEPQSSFSPDRSEPERPRSRRNSLTSLFRRRTSVDDTRQDSSQSLSPPRNRGSVSQDRPTNFHRRPSVISKKVIQQHGEYINLEGGLDICLNLEVSQHDPAGITTPYRLLVPALHYTEPEPGALEKQRRKSRGVFGVFGGGGAKRKTKIGDDGYSVSGSESGSELGEISDEDEEERTRQRYKVGPRILIPGFGSRNKRNSGSAQAVPPEKRLSSGYHTNSYEGPQPDPEQTDGSRGLAPAETQERDRRQSMDVAPNGQKGPAWESVGARRHVSAPQYPSTTTTTHTTTSTTVIPSRSASTRKQPTYNSSHKSQLPAAPAPEPEPEQNVQRRSMGGILNQHSHDKKWEHEAHKRSKRESYPPHPAIVGAGPGSPYSRDGPAGMQARNDVAGDYFASGGRDAQSYPTSAPTSRGGGGPTITTTTTVTPATTKRTPVEKEYAVKPVQRAEYAARSGYDQRTSSYPAYPQRNGNAGVGARYLGGGRYEDSDEYDSLDDSEEDDRDGRDGRRVYGAKGGGEGGRRYSGEGDVSQDSFVVPKKKKRKWQIWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCW7</accession>
    <name>A0A9W8YCW7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_002808</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCW7"/>
    <dbReference type="OrthoDB" id="4748970at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001102">
        <property type="entry name" value="Transcription factor IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051061">
        <property type="entry name" value="Zinc_finger_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179:SF13">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="62"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="92"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="126"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="154"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="186"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="305"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="20"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4374069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="565" mass="63924" checksum="886B5A0114D34411" modified="2023-11-08" version="1">MTVKRKALGNAGIVPQKKSCIGAVEEPTDEDSASDGSGDYQINYTDTGERRAESEARRQWNYVCPVDGCAQRFNRPCRLETHMRSHNKERPFACTEPGCDKTFPRKDHLQRHLKNAHNDPVVERTFTCDWRGCGKSFTSNGRLQRHREVHETKFYCTGYPPCYEAFRKEKALAAHIKSQHEEVKPYPCTFVDAETGEKCTSGFQTEAAMHRHMRRAHLGGEDEGHFCMLCAPPGVDMEAIQTESGETIMMPTQPLSFTTKEELLAHTEECHPAICSECGQKCKNATALKTHFDTVHADPAEQPRFPCPKSDCDSVFNRKHNLTVHIQAVHEKQFNFFCSADALQTSKHEDLKNWNGENACGAAFKAKSSLDQHVRTHHLGLGNRKQLRKAAKSKKKPDPSMLTLLTGVGYEEGREVPCLVKTCEYRFYMDRDLRRHLRAAHNIPEDDVEEMIQERDAMTGGQFWIGGVEDPMNMFESTEPSMPQTPAPYFAEAMMPDVEGQSKSMNPQFGYFDQQFDDLSMLNEEAEMDMAMGLTDVAPASNVQEGLQWEMLAPVEQYNMGNAQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD38</accession>
    <name>A0A9W8YD38_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Acyl-CoA dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_004120</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD38"/>
    <dbReference type="OrthoDB" id="10016597at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046359">
        <property type="term" value="P:butyrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00567">
        <property type="entry name" value="ACAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="6">
        <location>
            <begin position="7"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="5">
        <location>
            <begin position="261"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4372346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="431" mass="46674" checksum="D04D640512206173" modified="2023-11-08" version="1">MVNFNLTPEQKALRTNARAFAQNVLSTAPTLYSHLPTQTQRFQSTRPIYQAAVSAGLIKGQVPLPLGGSSTGLIDAAIVVEEFHAVEPSTAITILGTGLGLTPLILAGSKEQHEKFLTPFLELEGDRMASFVHSEPGGTANWLEMGGKGLGTTAWREGDEWVLDGEKLWATNSGGWDNRGADLQCVVARESKPHTPQDPDSDPSSHILILLVTREDIARNPPAAYQILSDPDLGGHRSVNGPHSRFTAFRVPSSNLLCAPGQGAAIVERTFGMSAAIVGAMCVGVMRHAFEAALAFCKSDDRGGVKKIVEHQSVADRLISAKCKIEAARALTWKAMCVLEGGDESVGWEQRLEVALETKIWCGDQARMVVLECMDVVGMKSYAKDMPFSTLLEDAACLPLFDGGNVGVRRRQLEKIFQRDDYEPWGATYPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDI4</accession>
    <name>A0A9W8YDI4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hsp90 chaperone protein kinase-targeting subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CDC37</name>
        <name evidence="11" type="ORF">N0V83_002000</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC37 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDI4"/>
    <dbReference type="OrthoDB" id="440202at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.610:FF:000002">
        <property type="entry name" value="Hsp90 co-chaperone Cdc37, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.610">
        <property type="entry name" value="Cdc37, Hsp90 binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004918">
        <property type="entry name" value="Cdc37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013873">
        <property type="entry name" value="Cdc37_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013874">
        <property type="entry name" value="Cdc37_Hsp90-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038189">
        <property type="entry name" value="Cdc37_Hsp90-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013855">
        <property type="entry name" value="Cdc37_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12800">
        <property type="entry name" value="CDC37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12800:SF4">
        <property type="entry name" value="HSP90 CO-CHAPERONE CDC37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08564">
        <property type="entry name" value="CDC37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08565">
        <property type="entry name" value="CDC37_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03234">
        <property type="entry name" value="CDC37_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01069">
        <property type="entry name" value="CDC37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01070">
        <property type="entry name" value="CDC37_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01071">
        <property type="entry name" value="CDC37_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101391">
        <property type="entry name" value="Hsp90 co-chaperone CDC37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cdc37 N-terminal" evidence="10">
        <location>
            <begin position="2"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc37 Hsp90 binding" evidence="9">
        <location>
            <begin position="205"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc37 C-terminal" evidence="8">
        <location>
            <begin position="418"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="105"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="201"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="415"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="156"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="240"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="415"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4374922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="524" mass="58495" checksum="4B250CB742D95B97" modified="2023-11-08" version="1">MVINYSKWDALELSDDSDIEVHPNVDKKSFIRAKQAQIHQERDHRRHQIKTLKYERIINDGLTERVDRLLTALKSHRAKQAEGNGANDDQLVFQAMMESMMDMKIDKDGGSDRPPPPPEGVHEHIKDKPTYPQMMASLVDAVKKDIDASKSDEPRYDQFIKGLEKEKARIEDLQQQLYAKLAEVEKEEKKHITSEDIHEGFSYSNVRKGEEPKPVASSSSSSAPKQSAPSSTVELLNAPKRPEASRTDTGDSGAEADVERGTAIHDEDEDNEDDITASPLAQSFAKIRTGDWYACLQFLMQHPEIMAEKETDGLLVEAFNSELDGKPKHARQCVHQGLLLQYCRQLGGRQGVELFFKRIQMKDHQAGKMFNDDVDSTYGRIKTRAAEILKERAENPQSAEGVEQIQLHAVDPGTQINISVPPAQSTSSDPEERQAEEQARQIFDSFPPGLQRALESGKLDEVNKVLAKMSVEEAEEIVEKLGAGGMLSLEQGVIDATTEEGQRAMEEIEKTRKFPGGGGGQTQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDS4</accession>
    <name>A0A9W8YDS4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CDC54</name>
        <name evidence="14" type="ORF">N0V83_002048</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the MCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDS4"/>
    <dbReference type="OrthoDB" id="10251574at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097373">
        <property type="term" value="C:MCM core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17755">
        <property type="entry name" value="MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000217">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008047">
        <property type="entry name" value="MCM_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF66">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21128">
        <property type="entry name" value="WHD_MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01660">
        <property type="entry name" value="MCMPROTEIN4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="13">
        <location>
            <begin position="594"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="325"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="52"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="125"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="327"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4374969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1014" mass="111277" checksum="830747CC249DE392" modified="2023-11-08" version="1">MSSPAKRRSQRSSASATPRRSARSGNSQVPSSPAPVGPDEQLQSEASQASQRGSQATPRNSRLQTASTQSPLFFRSSPVNPSGSGAANGAPESDGGATPRASGMTIGDSSPIHYASSSSPGRARNAQNTDIRSSSSNLFVHGSESAARNRRSDIHSDVFGTSSTNRRRRLFVDENGMSVHDASDAPTFSNLNPDTSDADVLGGNSSRVIWGTNVSLVDARHAMRDFLMNFQRKYRMIQDGELEEGMSLPADHPAMAREYVDIMKMMLELGVTPLNLDARNLKAYPPTRKLWHQLQAYPNEIIPIMDVAIKDTMLELAEKRMAEMRTQFSQAQRAAQPRARDSSSLPPMLSSDAPTPGAPSPAPVADIPNLVSEVDQKTYNVRPFGLDKTINLRELNPGDMDKLVSVKGLVIRTTPIIPDMKDAFFRCSVCNHTVRVDIDRGKITEPTKCPRAVCESPNSMQIVHNRSGFANKQVIKLQETPDDMPDGQTPHSVSLCAYDELVDVCKAGDRVEITGIFKCNQVRINPRQRSVKNLFKTYVDALHIQKVDKKRMGIDVSTIEEELAEHAAGDIEETRKVSEEEEAKIKATGARPDVYELLSRSLAPSIYEQDDVKKGILLQLFGGTNKQFEKGGSPKYRGDINVLLCGDPSTAKSQILQYVHRIAPRGVYTSGKGSSAVGLTAYVTRDPETRQLVLESGALVLSDGGVCCIDEFDKMSEATRSVLHEVMEQQTVSIAKAGIITTLNARTSILASANPIGSKYNVNLPVPQNIDLPPTLLSRFDLVYLVLDRIDEQNDRRLARHLVGMYLEDAPENASKHEIMPIEFLTSYISYARTHCHPKITEPASKALVDAYVAMRALGADIRSQERRITATTRQLESMIRLAEAHAKMRLSEEVTADDVHEAVRLIKSALKQAATDARTGLIDMSLLTEGTSTGDRRRKEDLKRAVLAALDELASAGQTVRLSELNKRVREGASEQIENQEFLEVLRSAELEGAVQLSGEGPRRMVKRVVGNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEA6</accession>
    <name>A0A9W8YEA6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1688-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_002340</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEA6"/>
    <dbReference type="OrthoDB" id="2153176at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012469">
        <property type="entry name" value="DUF1688"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31687">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31687:SF3">
        <property type="entry name" value="PROTEIN URG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07958">
        <property type="entry name" value="DUF1688"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4375254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="47643" checksum="34266BA706FCBA2E" modified="2023-11-08" version="1">MDTKVKYLLSLDAVRERAKLVGNAAQDGKLSHFDVHEERLSDVADFVTSLIKRDYGPDKFDTIPPHGRWQHFEVGNVPRIADVIDEWKTNGADDKEITRRLIDLFFVSVLLDAGAGDHWRYVEPGTDRKYERSEGIAVASFYMFKDLAFTANKNAKAPTVDGKGLEQLKTDVMAEGFQISDSNPILGLETRADLLRSLGKSLLAQPEVFGKEGRPGNVVDYLLKTTDDPSALNVVVFWDILQALLIPIWPKDRTVVNSHSIGDAWPLSTLKTQPTSTASDDSSYIQPFHKLTQWLAYSLMVPFQRILGIQWTGTDSLTALPEYRNGGLFVDMGVLSLKKDTLEAGLQASGGDLPMFKAGDDVIVEWRAMTLVLVDKLYGMVLKKMDGVHLTMAQLLEAGTWKSGREVAAKKRPQTKSSPIIIESDGTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YET7</accession>
    <name>A0A9W8YET7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Delta(24)-sterol reductase</fullName>
            <ecNumber evidence="2">1.3.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_001387</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.72" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YET7"/>
    <dbReference type="OrthoDB" id="415825at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000246">
        <property type="term" value="F:Delta24(24-1) sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050614">
        <property type="term" value="F:Delta24-sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000031">
        <property type="entry name" value="FAD binding domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040165">
        <property type="entry name" value="Diminuto-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10801">
        <property type="entry name" value="24-DEHYDROCHOLESTEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10801:SF10">
        <property type="entry name" value="FAD BINDING DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G14300)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4376106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="497" mass="57644" checksum="F9BD83A74F80A1F4" modified="2023-11-08" version="1">MDRHQRIVAQVASKVKEYFTRKEPFRIQHGSTNSTRPNLKKSVVDISSLRNVVSIDSKSKTARVEPNVPMDRLVEATLPHGLVPPVVMEFPGITVGGGYAGTAGESSSFKFGFFDRTINEVEMILADGEVVRASEKQNKDLFRGAAGAVGTLGVTTMVELRLVEAKKYVKTTYYPTRSVAQAVKEVREHTQGEKGDRHDYVDGILFSRDHGAIVTGEMTDELPPNTKPQTFSNPWDPWFYLHVEAATRTSEKPIIEYIPLAEYMFRYDRGGFWVGRSAFSYMRFPFNKYTRWFLDDFLHTRMLYRALHASGIATRYIVQDMALPYPNAERFIDYTDNTFNIWPIWLCPLKQSEQPTMHPHTKGELKDDQMLNIGLWGFGPNDPDEYLRKNRALEKTLGEMGGMKWLYAQTYYNKDEFWAQFDREWHEELREKYNAQALPGVYDKVHVDIKKYTMMANKDWGLRMRNIWPLGGFWGIWKAIQSKDYMIHRNSTWRSRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEY3</accession>
    <name>A0A9W8YEY3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein N-terminal and lysine N-methyltransferase EFM7</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Elongation factor methyltransferase 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">EFM7</name>
        <name evidence="6" type="ORF">N0V83_001462</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">S-adenosyl-L-methionine-dependent protein methyltransferase that trimethylates the N-terminal glycine 'Gly-2' of elongation factor 1-alpha, before also catalyzing the mono- and dimethylation of 'Lys-3'.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. EFM7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEY3"/>
    <dbReference type="OrthoDB" id="46564at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071885">
        <property type="term" value="F:N-terminal protein N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03223">
        <property type="entry name" value="Methyltr_EFM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025784">
        <property type="entry name" value="EFM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF10">
        <property type="entry name" value="PROTEIN N-TERMINAL AND LYSINE N-METHYLTRANSFERASE EFM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51560">
        <property type="entry name" value="SAM_MT_NNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5 6" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="82"/>
            <end position="84"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4376181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="252" mass="28506" checksum="AA9B3F22C8042A63" modified="2023-11-08" version="1">MASEADEESLDLFQEPADFYEPEKQATFASHQLLSGKELKVRLVGHNPLWGHFLWNAGRTISAYLEERSGELVKGKTVLELGAGAGLPSLVCALSGASQTVVTDYPDAELIENLRYNIEHCELLPKPPRIAAEGYLWGAPVEHLMKQLSNEKGFDVLILADLLFNHSEHGKLIKTVELTLKKSSASRAYVFFTPYRPWLFEKDMAFFDLARESGFVVTKTFEKVMDKVMFEEDPGDELLRRTVFGYELQWVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF22</accession>
    <name>A0A9W8YF22_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acriflavine sensitivity control protein acr-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001329</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF22"/>
    <dbReference type="OrthoDB" id="5380854at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF51">
        <property type="entry name" value="ACRIFLAVINE SENSITIVITY CONTROL PROTEIN ACR-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4376048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="55617" checksum="B3E8486B661AFE63" modified="2023-11-08" version="1">MQAVHSENGTKGEAKTHEGPTLIWDNESTSRQQSTLDIRRSFENAEIDTVQSAGQEEEEEVTMTVDRPLVDPLVNDLDQHSRYYLYHFATQLCGVMVVYDVPGRNPLRDIIPATSAYPLLLQIMIANSAFHVFNISCNPMGQSAYQGERSPTVVAYHQAVSRFGGSLKSSYKDALVAKQQALSLLAQNVASVNEFNIDIILATILMFVNYALVESGRDKWKVHMDGALKLIQRLGEPPYLQRPMSRLRQTILSDFLVFYILGSTFSFTTLPAMIPDTIELEPILRYAETNNYLSCPGPLLRIMIESFALPDSHYAPCNTGAHNNIQEQVGKLLQRALDFDPVAWSRDFEPASPFEDIQQRIRIAAAHRSAVCIYLARVLPSTNPLVDPTSETALVSLTGLADDVVHHISHLKSGDALFKSICWPLFLAGAESEDAAQREWIMNTLDELYGIMFWGYLHTSKRVLEEIWKFKDRAVAGADNCWVEEVKHLGNEILIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF81</accession>
    <name>A0A9W8YF81_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_001614</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF81"/>
    <dbReference type="OrthoDB" id="167398at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000293">
        <property type="term" value="F:ferric-chelate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF3">
        <property type="entry name" value="REDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G13750)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="12">
        <location>
            <begin position="347"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="556"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="565"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4376331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="677" mass="75194" checksum="ED2CC3C9582C4E53" modified="2023-11-08" version="1">MMDTKLLLPRVHIQNHSDGGTSVPHWGYADRVVPCTNDAGSCAYLDVVYNAHDVGMYYTGIIWATIGGILIVWAVGRHFARSQPSGVQLPVDAGEGVKESRSAFERLHRAITAYARQYLLPDAARPIFGRVSRTQVLTLVVLTGYLTIFSFAGIVYAKWVTPVKKMPGVYNTRTSLGPWADRVGVIAYALTPLSVMLSSRESILSLVTGLPYQSFNFLHRWLGYIIFAQSGLHTIGWCVVELRLYQPQPSVGKEFIVQPYIIWGAVAMILLTLLLVLSTPWGIRATGYEFFRKAHYVLAMIYIGACWGHWHQLEAFLLPGLLVWFIDRGIRLVRTALLHYNFLPSGHMGFRAASTNITFFTDEINGDVVRLDFDHPHDAWDVGQHFYLMFPESSIWQSHPFTPLSSPVYGADSQRHSYIFRAKKGETRKIAALSARRTAHMASHTESHEGLMPDAKLSVVLTGPYGERTLQSLVPNSNILCIAGGTGITYVLPVLLSLASQPQSPNRHMSLVWVVRRRSDIDWVAPELAILKRKCPNLRTRIHIYVTRASEQVQHGHDSSVPAISEKRTANECEKEIEPSSNSTSSSASISSALSLHGVSKADLGLNHYQARPDLSKIVGEFVEGSVSGGTDVFASGPGEMISDLRRIVASVNKGGEVWKGVERWDVGLTCDDRLEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFE1</accession>
    <name>A0A9W8YFE1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxidase FUB9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Fusaric acid biosynthesis protein 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_001333</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFE1"/>
    <dbReference type="OrthoDB" id="1925334at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02809">
        <property type="entry name" value="alpha_hydroxyacid_oxid_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000056">
        <property type="entry name" value="hydroxyacid oxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF149">
        <property type="entry name" value="2-HYDROXYACID OXIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000138">
        <property type="entry name" value="Al-hdrx_acd_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00557">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0288">FMN</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="9">
        <location>
            <begin position="6"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="195"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="200"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="85"/>
            <end position="87"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="330"/>
            <end position="334"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="353"/>
            <end position="354"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00073420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00083297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4376052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="411" mass="45251" checksum="B57D59FD03B2307E" modified="2023-11-08" version="1">MANSKAHDLNCLTISELEALAAQRMDKQTRDYYNEGADSGSTLAENISAYQKYRIRPRVLRDISSIDTSVTVFGHKNSIPLGVAPTAMQCLAHPDGETATARACKEMGVVMGLSSFSTTSLEDVKRSLGDEHPGALQLYLFEERQKSRQLIQRAKKAGYKAVLLTVDTPVLGRRNLEIRNQFKLPKHLKIANFMQDEERDQGVDNTEKETGANESRKRSAASEAGYQEGSKRVLPSGPITFHSHAPNPKLCWDRDIEWLKQECYPEMEVWVKGIATAEDALLACHHGVDGIIVSNHGGRQLNGALATIDALPEVVDAVRSQSSKIPIHVDGGIRHGTDVFKALALGADFVWVGRPVLWGLAYKGEEGVKLCLKLLSDEIRLCMGLAGVTKVEDIGKEYLVKIDRSGFVSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG50</accession>
    <name>A0A9W8YG50_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001668</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG50"/>
    <dbReference type="OrthoDB" id="4436466at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="260" mass="29010" checksum="9D90A6A960D97857" modified="2023-11-08" version="1">MNTISPSPQITKRLSGPKRVLGVVVGVTAGLGANVLLGYSVASFYTRNTKFVPFDTSSSDFTTSTFKSHNPSGNAPVCIDHAVKEIPYGKLPEKYWAKSSGGSGKISVDQGRLATDFCRGVWSGVAYRVQRKYLERKYRALPGREAHLWDVKELEDSEYKVGTVITDHFEVVEHTNDKVIVRCGDSPLNADHRPSDGLFAMEVSKDDDKQIATFHLKSVFVNTTPEGKGLEPLPWKFQLAHRWYTKLWMESATRKVLKDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG62</accession>
    <name>A0A9W8YG62_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001486</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG62"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="261" mass="29222" checksum="772F22CCE3AA9ED8" modified="2023-11-08" version="1">MSTSASRTTEQKIREAQNRAFEKLLTYISAIHTRMTSNLSHTEPTAALVAEALLAHARLYTLDSALSPSIVVEELSDREKVLKELSARCHAQDLVAEEFEEKLRNEWKTLNKEKGEGSAERRWEWLRGKVAEVNRMVVEWKKDIDAVFERVGRQGLSKVKFLVLEEVGVEAEVGERGMIGAMTGEEAVEEAAEDTAEDTAEDTAEDTAEDTAEDAPEEAPEEAPEEAPEEAPEEAPEEAPKETEGWSIQLRKQWGKQQKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGE1</accession>
    <name>A0A9W8YGE1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA polymerase eta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">RAD30</name>
        <name evidence="12" type="ORF">N0V83_001853</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGE1"/>
    <dbReference type="OrthoDB" id="5723at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009314">
        <property type="term" value="P:response to radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.100:FF:000009">
        <property type="entry name" value="DNA polymerase eta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.60:FF:000008">
        <property type="entry name" value="DNA polymerase eta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000014">
        <property type="entry name" value="Polymerase (DNA directed), eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="entry name" value="DNA polymerase, Y-family, little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017961">
        <property type="entry name" value="DNA_pol_Y-fam_little_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052230">
        <property type="entry name" value="DNA_polymerase_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873:SF1">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11799">
        <property type="entry name" value="IMS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21704">
        <property type="entry name" value="POLH-Rev1_HhH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036603">
        <property type="entry name" value="DPol_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="Lesion bypass DNA polymerase (Y-family), little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UmuC" evidence="11">
        <location>
            <begin position="70"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="519"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="547"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="581"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="608"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4374777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="646" mass="72447" checksum="F10F1FA8E86C95A0" modified="2023-11-08" version="1">MGLSKRAYTFKKIRVSGPLWPNCSMSSPAPNFASSPFSITSPRRIPKSKFTYKQLHQLKSYSPQTPLRVIAHVDLDAFYAQCETVRLGIDPSKPLAVQQWQGLIAINYPARAFGLSRHVTSTEALKQCPDLIMQHVATWKEGDEKWAYHENSFKEMATHKVSLDPYRMESRKILKCIKETLPEKEQRVEKAGIDEVFLDLSAQVHTVLLERYPELRGPTPYEDPTERLPKVPTTVLDWAADALVETGEEDGEDVDPDWDDVCMVIASEIVRDVRKDVKEKLGYTCSGGVARNKMLAKLGSGYKKPNQQTVIRNRAVKHFLSEMKFTKIRMLGGKLGDEAVAMFGTDKVKDLLEQPMDQLKKLGDDTGSWLYSTIRGEDNSEVNPRTQIKSMLSAKSFRPAINSFEQGVRWLRIFAADIFSRCVEEGVLENKRRPKTVNLHHRQGAQTRSRQAPVPQGKPLSEAMLFDLAKNLLAQVVVDGRAWPCANLSLSVGGFEDGITNNKGIGGFLVRGAEAKAMMSTGRSASGGEPPAKRRRTKGNIANFFGPRDDKNKGFDAARAVLMKAHSESTREEEAEDDFDEHEHEHDEDDHFHDPNEGDVERPHTPTPDRNASNNNDNLYRSDTPPSAQPQHNTHSLPVPPTTQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGQ2</accession>
    <name>A0A9W8YGQ2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Replication termination factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rtf2</name>
        <name evidence="3" type="ORF">N0V83_000241</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the rtf2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGQ2"/>
    <dbReference type="OrthoDB" id="247013at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006274">
        <property type="term" value="P:DNA replication termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16653">
        <property type="entry name" value="RING-like_Rtf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006735">
        <property type="entry name" value="Rtf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027799">
        <property type="entry name" value="Rtf2_RING-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12775">
        <property type="entry name" value="PROTEIN C20ORF43 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12775:SF0">
        <property type="entry name" value="REPLICATION TERMINATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04641">
        <property type="entry name" value="Rtf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="211"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="215"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="235"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="248"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="262"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="333" mass="35960" checksum="34CFD483E2C7C987" modified="2023-11-08" version="1">MGNDGGSIPKRRELVKEAAKALTSAQIKEAQTEKQEYSWSTDPLTRKPLARPVVSDAAGVLYNKDSIIEFLLKDDEDAEKAEMRKVGGVKGTIEGGFAELGTFGDRVKGLKDVVEVKFEIASEEQQQGEKARGEKWVCPITGTVLGPSAKAVYIVPCGHAFAGSVVKEVKGSVCLTCNEPYAENDVIPLLPTAPTDIARLNLRVKTLKDKGLTHALKKAPGSKKRKKGAEKETNGETTENGVTSKQPSSDEDKKKDKKDRSTSAAAGAASNGIKNASTASLTRKVLAEQEERNKRRKLAQNDNVNSLFNKSDHKPKSGNSADYMTRGFSIGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHF7</accession>
    <name>A0A9W8YHF7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Centromere protein H C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_000251</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the CENP-H/MCM16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHF7"/>
    <dbReference type="OrthoDB" id="2274804at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043515">
        <property type="term" value="F:kinetochore binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040034">
        <property type="entry name" value="CENP-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008426">
        <property type="entry name" value="CENP-H_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48122">
        <property type="entry name" value="CENTROMERE PROTEIN H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48122:SF1">
        <property type="entry name" value="CENTROMERE PROTEIN H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05837">
        <property type="entry name" value="CENP-H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Centromere protein H C-terminal" evidence="9">
        <location>
            <begin position="42"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Centromere protein H C-terminal" evidence="9">
        <location>
            <begin position="128"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="36"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4377426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="220" mass="24497" checksum="141B106E1CD4930C" modified="2023-11-08" version="1">MAAKADVEMADALDRVQDPQDYTDLLQTNHCDAFAFSESEKLALELYDQLQELELQKSLLQAQQSVTHDVSALSDDDLQEQLIIAQREAMEAKAEYEIRNSISHNVLVMDPVLKAVHGGEHTGFAENTAEQARIVADKKNRELSQTMLALAEEMKAQSAKDIEDPKLRDQVDAVDKELKESRRRMKTLKGVLSAMIVGSGINWAADEGLTELVLDDEEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHP6</accession>
    <name>A0A9W8YHP6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000654</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHP6"/>
    <dbReference type="OrthoDB" id="5153416at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="65" mass="6766" checksum="E31CDC3A56F029BC" modified="2023-11-08" version="1">MPPVPRAAAFPANSTVVASDLSSLAFAADGYWLNDLSGNGKAAFNSNPAAYKVFRNVKEYGAKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIQ5</accession>
    <name>A0A9W8YIQ5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001621</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIQ5"/>
    <dbReference type="OrthoDB" id="3798353at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4376338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="651" mass="74517" checksum="D327AB8829FDBB02" modified="2023-11-08" version="1">MPFRTLSTSLRARANQTLDRFRMRVQTRFEDERINIKSQYNVVKDTVKDTVLFVIATGLRKVVPSFKRTRFRDPACPLAAVRTLVSTSIRRPANCRQTTSRGVDRGRIDKEKRYPAVSPYYCSYPNVGRYHEPADSDELIEAFKLASEIRAQNEQRERLNRFRIAQQEADRRSAAVEARERAIAEQEAAELCPPPPYSSVERQRFYHERLKATEGTMVPVTGWPSGHSGIDPVMAEHNQRKFVQEFHDQVWACQVEEHRQLAQQEEERQRILGLLEVGRARYMKHVEMLANYGPQLHMGINMMKVTLRHNNMSDLALLTREMITGILEPVHAIFQEMNENIPLEEEGAHILKNKWPIKHIDEVYIFLKNEDSLPPELVYFLQGIEAICKPISNPTPFINPLNPQPYPVEVPPTPAQNITTVPKYDGPNLNLWNGLADALNDVLPQVSNVATAKYTQQQYQQQQYQQQQYQQQYQYQQQQEQQLPTIIISAPTGLPEALNDALPQVSSVAATQDTQKHQQHQQQHLPTIIVSTPSAPSSAPTPDSQEKQNLMQHMQSSLFDLQEDVQKLLEGETLPPTPRNGVRKTLQAFLAIANGKMELLGDDEGRLYKNEVQRVVTRIANLKNKAAKKAPQTKELDTLAYDVLNVWFSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJB2</accession>
    <name>A0A9W8YJB2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000798</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJB2"/>
    <dbReference type="OrthoDB" id="3924760at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="228" mass="25597" checksum="17D08AC704147800" modified="2023-11-08" version="1">MQATTDRPMINIAKVSEWLTKNANEQLYPRLFLMSPNGTLLAYSKPVDIKELRDQAALISMVWKDNCIGRQKITARSDSNQDSTLKPALKILTIETEDCNIIVRLLQPELLLILVGKLPPRKKQPFKITPEGSGDSRYPETEGPLSRPNSSSNLHQSDGTAEISRPTTGEEDIKRKAPSLVSTLSQREKDIRVGTLHVQRKRLDALVDYILKDFEDTGFVMPADSDLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YKJ8</accession>
    <name>A0A9W8YKJ8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000817</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YKJ8"/>
    <dbReference type="OrthoDB" id="3801219at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="254" mass="28627" checksum="E0BB1E186C32B1CB" modified="2023-11-08" version="1">MSQPTSPPTAMRGQINTDDALLDKPSQARPSNSLEGTARVDGRMSTSRHADLSSPSKAQRTLLSLPAEIRLEIYSYLLPPLATPSEGEALHATCRQTKAEIESESAKRVENHLRVRIGPLPKNDPLHPIVYPIPATIEVGRKPSSMVFNLPIPSLEERKFTDSLASPGPDMPPLSDFHSVSLTYRLLDALPAYINEVTLSYGYHERHINIIWCVQVVTRFVDYFLSNRIIVERKDLNLRKFDVILHRDFRTCVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH28</accession>
    <name>A0A9W9CH28_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BAG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010845</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH28"/>
    <dbReference type="OrthoDB" id="417450at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00264">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAG" evidence="2">
        <location>
            <begin position="447"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="394"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="368"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4361904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="508" mass="55177" checksum="87F144C2F81E8595" modified="2023-11-08" version="1">MTKPRTLTSLVSDLARIDVRPLQPLADHQQSDATVKTPPHTSSLLQLLTTSSEARADTIQPCYFGRAQLHEAVTVASSNKLVDDLKKSFNFFKNHPYLGTTSSPPVSDNRDLPTDLLSRIIQTLQQSGLNDITEFITNPRFDDPTYLTALAIALAAIFVTMSWFSRSGGSGGWGGRFSPFGRPGNSPNSGVVNDSDFSYITSEDLRRNGAAQAPEIVDWDDKNPERETDVLIFKEKRTHYPTHFPAHSIRDGDLKIGTVRQAAAKKLGIDNPARIRMFYKGRNLKYDERTAREEGLRGDGSGSEILVTIGEAPVGGLAPGSADAAPRQWSDGEDEEDDDEDEVDSGANTTGKKKTRKRGGRKSKKKGSSSANASGTSTPGYVGAGAGAEYLPIPSHINAAPRPTSAPPQNPPRAATPVTAMGKLDAIASKFHTEFVPLAIQYMTNPPAEKAKREFEYKKLSETILTQIIFKLDSVETEGDPDARLKRKALVKEVQGMLNKLDEVGKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH46</accession>
    <name>A0A9W9CH46_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tripeptidyl-peptidase II</fullName>
            <ecNumber evidence="4">3.4.14.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">SED2</name>
        <name evidence="18" type="ORF">N0V83_010905</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Secreted tripeptidyl-peptidase which degrades proteins at acidic pHs and is involved in virulence.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal tripeptide from a polypeptide.</text>
            <dbReference type="EC" id="3.4.14.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="15">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
            <location evidence="3">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.10" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH46"/>
    <dbReference type="OrthoDB" id="409122at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04056">
        <property type="entry name" value="Peptidases_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11377">
        <property type="entry name" value="Pro-peptidase_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000015">
        <property type="entry name" value="Tripeptidyl peptidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015366">
        <property type="entry name" value="S53_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030400">
        <property type="entry name" value="Sedolisin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050819">
        <property type="entry name" value="Tripeptidyl-peptidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218:SF35">
        <property type="entry name" value="PEPTIDASE S53 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218">
        <property type="entry name" value="PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09286">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00944">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51695">
        <property type="entry name" value="SEDOLISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 15" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8 16" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0843">Virulence</keyword>
    <keyword evidence="13" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040759926" description="tripeptidyl-peptidase II" evidence="16">
        <location>
            <begin position="18"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S53" evidence="17">
        <location>
            <begin position="210"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="15">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="15">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="15">
        <location>
            <position position="502"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="544"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="545"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="577"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="579"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4361964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="611" mass="66280" checksum="B70CD390D330B4E3" modified="2023-11-08" version="1">MRYALFAGLLAIAGVTCTTTYHIEHGSETVEHLRRVPEGWNEVGAPSQDHKLHFRIAVRSENRDLFERTLLEVSTPSHPRYGQHLKRHELKDLIKPRAESTSAVLSWLESAGVESRDVHNDGEWINFYAPVKRAEEMMGTSFKTYQSEVRPAIRKIRSLSYSVPKEVRSHIDMIQPTTRFGQIRAERSNVLTQEEAPFSVLAVNATCNTAITPACLADLYNFASYKIDPTADVTIGVNGFLEEYGRYADFAQFAKTFAPNAVGSNFSFTSVNGGLLDQNASSDSVEANLDVQYTAGLVAPNIKTNFYSTPGRGELVPDLDQPTADDNANEPYLDFFTYLVGLEDEKLPQVLSTSYGEDEQSVPAEYAKKVCDLIGQLGTRGVSVIFSSGDTGVGSACQTNDGKNTTRFLPIFPASCPYVTSVGGTTKVSPEQAVSFSSGGFSDLWTRPSWQDDAVAGYLKQLGSKWDGLYNPNGRGFPDVSAQGQSFRVVDKGRQISVGGTSASAPVFASVVALLNNARVAAGKPALGFLNPWIYSQGYKGLNDIVNGGSTGCVGRSIYSGLKAPYVPYASWNATTGWDPVTGYGTPDFGKLLRISQAGVYGGRRGLDGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH90</accession>
    <name>A0A9W9CH90_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_010862</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH90"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4361921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="242" mass="27305" checksum="ED2B4B846E56138E" modified="2023-11-08" version="1">MSQDPHLSATKGERLLTVQGLLRLEPAVGDPTWRPVTIELDSQDRRMDRRHLGRPAVTDDIMRLIATRGSRFSKEAVDAAVATLQEEDLEKRKEQLNLRATITMLLPPGEREENNLGMTYADVYSLFPDSAWSDKVWKCAFDTLPFSLGRTDIVVRNCNAVSCANHDITRAHVLISRLEGNHYFATIRKQFCQTIVAITTSDFEATDVPDIRKNHLSHLQGSFGHASIVRKLQSLNLKPTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHA4</accession>
    <name>A0A9W9CHA4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_010907</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHA4"/>
    <dbReference type="OrthoDB" id="3932667at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4361966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="617" mass="68556" checksum="67EE7A83FCD4F188" modified="2023-11-08" version="1">MAQSGIFSASIAVQPCDYGQIITERIGCHYLTQELKKREDATALSKKCQYRLNILNILEAACAQWTGPGQAGARKEDVDMLKDDNKSAEELFSKFISLAETLDFSKAVAMHFGGQASEERTSLTQAWDDAVEDAEGTACTSLAGKLEFLDCPSVRDCLLHPLLVALLAFRLGGPINAANTAYAHEWKLPELDMSEEQSFHMEGDSGDFFEDHRITLVWETQHGEAKSASGKHHIFLTGDATPQPLQILSPVGDIDNAPMTIIYDSKSAALSYDCPGSGAVRKSITLDIHLNTVADDDIQLLSTQYEQMDLKRLTLAKVLTSFPGVNYAALFHTLLFEPKSLNAIVAKLSTLEISKPEPPPDTAGHSLNQAFEAYRRENLARIPPTIKRMENDILITGMYGAPAVFLERLCVKACRSIHLPIGRNLFPQTPLEENIECARKFIRDLPRSIIEDRLAEYAPTLFGQYSRLDLMSTMTLHRTGTLIGQRCLELTSQGFVDAECLLPSIAALAPTFGKALNGPKEIDIVQEPWVDDHDLDVYGTRCLYLFWCADWLSCYLKNPEEGPFMLVDSEKALEDTRRMRRAIEHAAKSLLINWVAWGLFVEALPRGGFTVRQPRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHP1</accession>
    <name>A0A9W9CHP1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ADP-ribosylation factor GTPase activating protein, ER-Golgi transport</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">GLO3_1</name>
        <name evidence="8" type="ORF">N0V83_010083</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHP1"/>
    <dbReference type="OrthoDB" id="983479at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048205">
        <property type="term" value="P:COPI coating of Golgi vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08831">
        <property type="entry name" value="ArfGap_ArfGap2_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000013">
        <property type="entry name" value="Putative Arf GTPase-activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686:SF4">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEIN 3, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEIN 3, ISOFORM H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="7">
        <location>
            <begin position="10"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="140"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="257"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="343"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="260"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="283"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4362966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="477" mass="50603" checksum="87701A5FF2FE5C43" modified="2023-11-08" version="1">MALATKTQSQIIFTKLKAKSANKICFDCGAKNPTWSSVPFGIYLCLDCSSNHRNMGVHISFVRSTNLDIWQWDQLRIMKVGGNESATKYFQSHGGSAALASKDHKAKYTSNAATKYKEELTRRCAADARLYTNEVVITDVPDATGSDGNNTPAGEDDDFFSSWDKPTIKRPSNPPSRTGTPRAGSPFLKPGANGNGTDRPKSPLVGGSEASTPAAVPSAKPAVRKTTAGAAPKKNILGAKKKGLGAKKVVADGGLDFEEAERKAREEAERIEKLGYDPDAEAAEAAAATKSKAPEATTIASPTPVSPPRGGFGSTTKPERSTQDMERLGMGVARLGFGQVGAAKKPATQQKKMGGFGSVSKPVEDDSEKYAREKFGTQKGISSDEFFGRNAFDPSATAEAKSRLSGFEGATAISSNAYFGRPEDDIPEDDYGDLESAAKDFVRKFGITAGDDIENISAIVGEGATRLQGAIRSYLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIF0</accession>
    <name>A0A9W9CIF0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Component of the polarisome</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">SPA2</name>
        <name evidence="4" type="ORF">N0V83_009373</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIF0"/>
    <dbReference type="OrthoDB" id="5588096at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005826">
        <property type="term" value="C:actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902716">
        <property type="term" value="C:cell cortex of growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005078">
        <property type="term" value="F:MAP-kinase scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013724">
        <property type="entry name" value="GIT_SHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039892">
        <property type="entry name" value="Spa2/Sph1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056439">
        <property type="entry name" value="VBS_C3G9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21601:SF0">
        <property type="entry name" value="PROTEIN SPA2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21601">
        <property type="entry name" value="SPA2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08518">
        <property type="entry name" value="GIT_SHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23742">
        <property type="entry name" value="VBS_C3G9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00555">
        <property type="entry name" value="GIT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIT Spa2 homology (SHD)" evidence="3">
        <location>
            <begin position="127"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="GIT Spa2 homology (SHD)" evidence="3">
        <location>
            <begin position="186"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="153"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="222"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="614"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="693"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="347"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="223"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="307"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="614"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="696"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="715"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="756"/>
            <end position="766"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4363920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="921" mass="101208" checksum="80AC3C577472138A" modified="2023-11-08" version="1">MNTRNVALSPVSTDSQDWSPITRYQNIAGDNPYSPTLPPNTYGPSNTDGGMLNGMRPAANGNPSPPSSVGRSSDGTGLYASSLSDASMNNRKMQALEETLSEHFRVLKAYLGPYLNDEKGNPRPSRAKDKLTRLSAVQFQELSTDVYDESIRRELDRKRGGTGAPGNDTPQYLLPKNNFHPKRNQARQKLSTLPLERFRQLATDVFYELERRFPRFTGGEMVRSASPAASIASRISNRGPPSRTGTPNSMNGRPPPGPGYRGPPPGGPMGPPGALRPGSANTENSFGRPLPKTFQSNTIVPNKGTMVEDDDDSAGDDDDAFNLEGAAARRQTNKSTKSMSMAQDKMVADLQSQISELQGKLSSLEGTLRDRDGELERLQDNDRMRDGNTASERAEWDELRYSLEEKVEKAENLNSSLRSEIDKLRSENEGVERNLRAQIADLESRPPQQAYENGDGEWRQRCEELERELSEQQQVTEEVRRDASVFLQEMRELSSRSDAAVEKEERLTTQVSSLEGELKDWKSRYARAKTQLRNLKASSIGLSSLASPDITNYARDPSFNTPDGLVKDVHVTKFQLSIDELLQTARRGNSEQTLESMRHVVKCVRAITGDIDSTPLSQVQSPGSSISGDIMASPEKKQAKLKSRVSATANNLITATKNHASSSGLSPVSLLDAAASHLSTAIVELVKHVKVRPTPEDELDRDEEDEDRPMPLKPTTYSAYSAFSPNNANGNTNGASPVYGHQRGRSSKGGSSDTTGYSAYSSPYSGYDRQSAGMSGMNGQSDSGMMEFKNYLEDQTALLVQSIQPLVNLIRSNPVSTPADEQQIYDYIQDISQAVEDTGNRTYNAVNQLSNPALKKHAIPVVEALDECRQSMLSVDVRGGGRERIPPLAFKTARALKELVLRVDRIESGELTADQTLKTDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIL4</accession>
    <name>A0A9W9CIL4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial</fullName>
            <ecNumber evidence="6">1.1.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Isocitric dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">NAD(+)-specific ICDH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">IDH2</name>
        <name evidence="16" type="ORF">N0V83_009049</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-threo-isocitrate + NAD(+) = 2-oxoglutarate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:23632"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Octamer of two non-identical subunits IDH1 and IDH2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.41" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIL4"/>
    <dbReference type="OrthoDB" id="10261637at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000003">
        <property type="entry name" value="Isocitrate dehydrogenase [NAD] subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004434">
        <property type="entry name" value="Isocitrate_DH_NAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00175">
        <property type="entry name" value="mito_nad_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF34">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="10 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="15">
        <location>
            <begin position="54"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4364455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="385" mass="41790" checksum="E1E4963530C82CF0" modified="2023-11-08" version="1">MLAARSLAAPARQCLRQANRSSRWAPALSQVSARTYASEVAKFKGTKGNDGKYNVTLIEGDGIGPEIAQSVKDIYSAANVPIKWESVDVTPRLNEDGKTVIPDEAINSVQRNLVALKGPLATPIGKGHVSLNLTLRRTFNLFANVRPCRSIAGYKTPYDDVDTVLIRENTEGEYSGIEHIVVDGVVQSIKLITRDASERVLRYAFQHARDIGRKKVRAVHKATIMKMSDGLFLSTAREISKEFPDIEFDAELLDNTCLKMVTDPVPYNDKVLVMPNLYGDILSDMCAGLIGGLGLTPSGNIGDKCSIFEAVHGSAPDIAGKQLANPTALLLSSIMMLRHMGLNQEASNIEQAIFKVLAEGKTITGDLGGKSKTYEYADAVIKALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIR6</accession>
    <name>A0A9W9CIR6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009051</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIR6"/>
    <dbReference type="OrthoDB" id="630895at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051531">
        <property type="entry name" value="N-acetyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43792">
        <property type="entry name" value="GNAT FAMILY, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G00765)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43792:SF16">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="96"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="229" mass="25098" checksum="3BEF6FF838453A66" modified="2023-11-08" version="1">MVATEFHINTPRLVISHLDSSQDSHCDFLINLYNTEQHRALNTALPDREAARKKIDSYDLVRTLGYGRYLVSLKRQTPPLTSSSSGSALADADAEAEAEAEAEADNNIPFSERVKAYTEIGVVSLNSRQFEGAPRAPDVGYNILPAFQRKGFATEAAAALVRWFEAEKGQREFFGFCDPGNEGSKGVLRRIGFEERGVREIRGLYSNGGVIVGMVFSKGLTGSLEAYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ48</accession>
    <name>A0A9W9CJ48_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Ubiquitin conjugation factor E4</fullName>
            <ecNumber evidence="13">2.3.2.27</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">UFD2</name>
        <name evidence="13" type="ORF">N0V83_008675</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ubiquitin conjugation factor E4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="13"/>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ48"/>
    <dbReference type="OrthoDB" id="20295at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034450">
        <property type="term" value="F:ubiquitin-ubiquitin ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000055">
        <property type="entry name" value="Ubiquitin conjugation factor e4 a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019474">
        <property type="entry name" value="Ub_conjug_fac_E4_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045132">
        <property type="entry name" value="UBE4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931:SF2">
        <property type="entry name" value="UBIQUITIN CONJUGATION FACTOR E4 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931">
        <property type="entry name" value="UBIQUITINATION FACTOR E4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10408">
        <property type="entry name" value="Ufd2P_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0697">Rotamase</keyword>
    <keyword evidence="7 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="12">
        <location>
            <begin position="1010"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="95"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4365059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1101" mass="124494" checksum="4B1B1E3C12592BFC" modified="2023-11-08" version="1">MADETPPPPPPPPEQDSAMATSDADKVSSTHPPKRPADENLAQIRAKRLAKLGGPTPPSGASRPPSVPPEASTPSSSSPKPAEDQSRAPQPPAEATQSPNPFSQLGVNTDAPPKTRINIKPAPDQPSSTSTPSRPPPKAQDVSLEAWEDRNISAIFRVTLDQSRTHDTHGHKLYFAAGAKSDLEDEARPLRLTTDMLDSVILESASSHVHGTALEYLLGCWKRVSKLSKSIANKNDPKFEIAKEARRLCFSYCIFAATMPDMFGEETPEMNALGDALILGPDHDQGICYDFLTEASQRMGEDDTVKDALVGAMEDMSRRLSSSTMNGDYKPYMLALRVFMRFPPLVAALGQSETFLPAEIEAQDIETKTFLGPFFSLSPMQSSVALNFFSGSAATDKGLIANAQRAVRMTLQTHQEELLDITNSFIKNKETREKMLDWFALTVNKNHKRRAIQVDSKTVSSDGFMVNVTVILDRLCEPFMDATFSKIDRIDIDYLRRSPRVDIKDETKINADQQTSDEFYSHTVEGTNNFISEVFFLTVAAHHYGFEAANSKLSNLQKDVKWLEKELAKMEPERHKYMSNPGQLMVFDAHVRKVKDQIERGKSSILAIQGVLLDETTQARSMQLMRYVIVWLLRIVSPSGTFPKKELQLPLPTEQPVEFKCLPEYFVEDIVSNFKFITRWMPHIITTTQCEELVKICIALLRSSEYIKNPYLKSGLVTILFFGVWEIPGRSKGVLGDTLYGHAFAMQHLLHALMKFYIECESTGTHTQFYDKFNIRYEIFQVIKCIWPNTVYREHLATEARVNLEFFVRFVNLLLNDVTFVLDESFTAFKEIHEISRALKNPPADMDQNTRQEQEEKLSAAQGKAKSYMQLTNETVAMLKLFTEALADSFTKKEIVVRLAHMLDYNLEALVGPKKSNLRVENSEDYGWNPRNMLAEVTDVYLNLQNKESFLESIATDGRSYRPEYWDEAYKILQRFKLKTPEQMQEWQNMAERIKTAKDAADMEEADLGDIPEEYTDPLMASLMEDPVILPMSKQVIDRSTIQSYLLSDPRDPFNRTPLKIEDVIDNTELKEEIQTWKANLLKKKLAERNAATAGEPMDTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ62</accession>
    <name>A0A9W9CJ62_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mannosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">GPI10</name>
        <name evidence="14" type="ORF">N0V83_008855</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the third mannose to Man2-GlcN-acyl-PI during GPI precursor assembly.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 22 family. PIGB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ62"/>
    <dbReference type="OrthoDB" id="416834at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF4">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="268"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4365237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="549" mass="61555" checksum="B4D3258B9A333D21" modified="2023-11-08" version="1">MPPSSDSARDTTPSGTGAPPPSRLTSYRDSRLGVFSLLLALRIVNALTLRTFFQPDEFFQSLEPAWQLAFGQTSNAWITWEWRTQLRSSLHPALFATVYRVAADLGAFCGLNLPARAELLLAAPKLIQAVFAALLDCYTWKLAEKAYGRGSRTALTTPLRSFGYKHAAASTTEFEWSSDSEVTDNARSAVLACSIASDRLYYEVWTLPPLRFLYFNIAQSLAVFYGSNRADYYITEGLPLLLTTALPFAAIGIWQSLHGSEPERSPMTGESSTSSPVTTPTETAIQRSILKRLAWASLVVTLTLSLISHKEVRFLYPILPFLHLIAAKPLASFLPTHAPISRKTVIFLLFATNLLLAGYASQIHQRGVIDVLSYIRHKHETRNGLSASSSSTSQTRPISNTTVGFLMPCHSTPWRSHLIYPQISAWALTCEPPIDIPLPERSTYLDEADEFYLKPGPAAWLRSNMESTDTIQSGGSRSGQHWMKNDPRFKRKYKREWPQNLVFFEQLEGTLKEHLDGTRYTECWRGFNSHFHDDHRRVGDVIVWCLDGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ66</accession>
    <name>A0A9W9CJ66_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flavoprotein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_008871</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HFCD (homooligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ66"/>
    <dbReference type="OrthoDB" id="1532798at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF6">
        <property type="entry name" value="PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="4">
        <location>
            <begin position="41"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4365252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="274" mass="30587" checksum="B5F98C748C73CA8B" modified="2023-11-08" version="1">MSAEIHDLKPLNIKPVQHQHHHEPQTQDPPAPFNLHDDKIHILLCASGSVATIKIPNIITALSKHENIRIRLIFTAAAANFLQGQSAEQPSIAEIAAYPNVDGVYFDEDEWKEPWVRGNKILHIELRRWADMMVIAPLSANELAKITQGWSDNLLLSVVRAWDTTGLIDPVRKIPGVQWTPQVATGEILPPKKRILVAPSMNTAMWFQPVTKKQVRVLEEEWGVRNGGWFEVLQPMEKELACGDIGGGAMKDWREIVGVVEERLGFSSGGGDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ81</accession>
    <name>A0A9W9CJ81_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">GET complex subunit get1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 13" type="primary">GET1</name>
        <name evidence="13" type="ORF">N0V83_008767</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the WRB/GET1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ81"/>
    <dbReference type="OrthoDB" id="69461at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.660:FF:000006">
        <property type="entry name" value="Protein GET1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03113">
        <property type="entry name" value="Get1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028945">
        <property type="entry name" value="Get1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027538">
        <property type="entry name" value="Get1_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42650:SF1">
        <property type="entry name" value="GUIDED ENTRY OF TAIL-ANCHORED PROTEINS FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42650">
        <property type="entry name" value="TAIL-ANCHORED PROTEIN INSERTION RECEPTOR WRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04420">
        <property type="entry name" value="CHD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040979244" evidence="12">
        <location>
            <begin position="24"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="4"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="9">
        <location>
            <begin position="173"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4365149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="216" mass="24259" checksum="85E7BA1932D9AA6A" modified="2023-11-08" version="1">MPSLLLVMFTLQVLLHIINTVGASTLNELLWIVYNKLPTPTSGSAQKAQVLKKEVVRLKRELGNTSPQDNFSKWAKLDRQHNKAMAEYQKLDGSLRSHQATFTSAVSTLRWVGTQGLRFVLQFWFAKSPMFWLPAGWVPYYVEWILSFPRAPLGSVSINVWGIACASMIALVAEALAAAWVLVTKQPTPATTEKQREKEEAMAFKADQRPTGKKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJA8</accession>
    <name>A0A9W9CJA8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJA8"/>
    <dbReference type="OrthoDB" id="3794485at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="379" mass="42151" checksum="E269880C8EE6F872" modified="2023-11-08" version="1">MPLIKRVQPTSDETPPWSGMSFVQPVDSDALHEHLKIAYPQCTTLRERKHMAAIDFLVAELHQMQSKGPTFVAVEHSANDPAAPNPGALSRKGSISASLSPTSSAQFSTSVELAFQDRHSSTTPGRLLPKGQLTESPGQHLVFSAVDGRMMQPKTKRRMTAEEKDAYKETRKRGACRKCKRQKGKCTHVIDMNEGLGHTKDVKKATKRRTSQNSGVESQNPSKLAKVEHQFKTNQLKSNLPSQQDSSGLQHGPDNEVDGPSNQPTLQSDNMQWDQKLREMDQSELPRYVNHQVFMPPYGVSLPGNAGAEWTGYATPASVPTFNQAYIDYVENPGQCEEVYPMPSSHASLQPDLEYPSEYPGHEPWNENQEPHTRNADPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJN0</accession>
    <name>A0A9W9CJN0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small nuclear ribonucleoprotein Sm D1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">snRNP core protein D1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">smd1</name>
        <name evidence="10" type="ORF">N0V83_008433</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in splicing regulation. Facilitates post-transcriptional gene silencing (PTGS) by limiting the degradation of transgene aberrant RNAs by the RNA quality control (RQC) machinery, thus favoring their entry into cytoplasmic siRNA bodies where they can trigger PTGS. Does not participate in the production of small RNAs.</text>
    </comment>
    <comment type="function">
        <text evidence="7">Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the snRNP core protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJN0"/>
    <dbReference type="OrthoDB" id="9626941at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031981">
        <property type="term" value="C:nuclear lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097525">
        <property type="term" value="C:spliceosomal snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01724">
        <property type="entry name" value="Sm_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000008">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034102">
        <property type="entry name" value="Sm_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="9">
        <location>
            <begin position="2"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="79"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4365812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="129" mass="13745" checksum="3F1CDAD48EA83F68" modified="2023-11-08" version="1">MKLVRFLMKCQNESVTVELKDGTLANGTIASVSPVMNVSLKTVKWTVKNRNTITLDQMTVRGNQVRYIILPDSLPLDTLLIDDAPKPKNKARKEAGGDRGGRGGGRGGPRGRGGRGGGRGGGRGRGRGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJX5</accession>
    <name>A0A9W9CJX5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">3-oxoacyl-reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_007787</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJX5"/>
    <dbReference type="OrthoDB" id="1669814at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24321">
        <property type="entry name" value="DEHYDROGENASES, SHORT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24321:SF8">
        <property type="entry name" value="ESTRADIOL 17-BETA-DEHYDROGENASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4366152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="253" mass="27421" checksum="38388F51FB633E08" modified="2023-11-08" version="1">MPVDYDLKGKVIALTGAASGIGYETSVLLARQGAYLSLADLNEDALKEKVLEIEKVSTGKVFSTAIDVRKDDQVNAWIEKTVEKFGKLDGAVNLAGVIPKGINKDRVQDLDNEDWHFVLDVNLHGVMYCMRAQLQNMNQKGSVVNAASICGVIGFPKNAAYTASKHAVIGLSRAAAKEVGEREIRVNCIAPGLIEGPMQQKSVAARGGEQVWRQQITRRGTPHEVGALIAWLLCDDTQYITGTVQIIDGGWVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKB8</accession>
    <name>A0A9W9CKB8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_007482</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKB8"/>
    <dbReference type="OrthoDB" id="3649348at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4366952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="126" mass="13373" checksum="848A38C514CEF761" modified="2023-11-08" version="1">MSPMPIIVCGRNPNIAKNVRKGLLPEYDVIHVAIGLDAAISDIPLLFSGKTVPSNNNSDNLGSQNYAKKPVAVAVGGGFDDEMFEQMKAATEGKEVVWVRPDLSKMDGMPELGDVEAYAAASAVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKV4</accession>
    <name>A0A9W9CKV4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_007129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKV4"/>
    <dbReference type="OrthoDB" id="39175at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4367545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="221" mass="25065" checksum="9FF4E300377B8F54" modified="2023-11-08" version="1">MLSLNEALDDLGNLRVRADVIKVDFNLSPKEAKDCIDAFVKLMSSMVVPDIFTKSLDYNLLYALPTIVDSPYINIDPGLRVMYYNALYYGLQQIRGPGSPLMQAAYMKVLEAVPAWLEASTNTSMDGHTAALTSWTAINNQDYQLSWKFHCKSCHFIKLAGIDQLDVIPAKTLEEESERDALRFLYWHILSTDTLFRMFYGKPTVVRLHSVIRAEASLFVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL24</accession>
    <name>A0A9W9CL24_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DASH complex subunit DAD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Outer kinetochore protein DAD1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">DAD1</name>
        <name evidence="18" type="ORF">N0V83_006502</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DAD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL24"/>
    <dbReference type="OrthoDB" id="5566853at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013958">
        <property type="entry name" value="DASH_Dad1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28025">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28025:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08649">
        <property type="entry name" value="DASH_Dad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="15" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="77"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4368146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="105" mass="11783" checksum="EA33C2DDAF292098" modified="2023-11-08" version="1">MATTNRASTANGTHAAPEKSYYEQQREILVTEIAQSLELVLQNINKLNRSLEEVTQVGNEFAPVESLWSHFENVMAKDPNEQQGDAQEGATEHEGEETEVTEHQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLF2</accession>
    <name>A0A9W9CLF2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLF2"/>
    <dbReference type="OrthoDB" id="2537769at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012808">
        <property type="entry name" value="CHP02453"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36452">
        <property type="entry name" value="CHROMOSOME 12, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36452:SF1">
        <property type="entry name" value="DUF2461 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09365">
        <property type="entry name" value="DUF2461"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="123" mass="13519" checksum="48767CF527E03F86" modified="2023-11-08" version="1">MAGYRRVSGISMCGPPMLYPLDLQALFDSTSIEDFLGYDLDHKDIDLLRLRNFTIGRRLLDYEVVGPKGLDRVAELILDMVPFITYLNSVCMPDEDTSSSSEDESDAGSDDDNDSADADGDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLV3</accession>
    <name>A0A9W9CLV3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 6</fullName>
            <shortName evidence="5">eIF-6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">TIF6</name>
        <name evidence="6" type="ORF">N0V83_005657</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer. Associates with the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleolus</location>
        </subcellularLocation>
        <text evidence="5">Shuttles between cytoplasm and nucleus/nucleolus.</text>
    </comment>
    <comment type="PTM">
        <text evidence="5">Phosphorylation at Ser-225 and Ser-226 promotes nuclear export.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eIF-6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLV3"/>
    <dbReference type="OrthoDB" id="4155914at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00527">
        <property type="entry name" value="IF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.75.10.10:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00032">
        <property type="entry name" value="eIF_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002769">
        <property type="entry name" value="eIF6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00323">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10784">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01912">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00654">
        <property type="entry name" value="eIF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="modified residue" description="Phosphoserine; by CK1" evidence="5">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine; by CK1" evidence="5">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4369893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="297" mass="31952" checksum="BD8F450598BF4FDF" modified="2023-11-08" version="1">MPPLTVFQPHNSRQPTQTFSTRLCTLIPTSLLASDPSDTVTRSTNTARSKVMAVRAQFENSNEVGVFSTLTNAYAIVAVGASENFYSVFEAELQDVIPICHATIAGTRIVGRLTAGNKKGLLVPTTTTDQELQHLRNSIPDSVKIQRIEERLSALGNVICCNDHVALVHPDIERETEEIIADVLGVEVFRQTIADNVLTGSYMALSNQGGIVHPKTSIQDQDELSSLLQVPLVAGSVNRGSAVVGAGMVVNDWMAVTGLDTTATELSVVESVFRLGEGNGPSNINTTHKDAMVESFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM03</accession>
    <name>A0A9W9CM03_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_004879</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM03"/>
    <dbReference type="OrthoDB" id="648861at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF49">
        <property type="entry name" value="LYSINE BIOSYNTHESIS REGULATORY PROTEIN LYS14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="104"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4370361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="580" mass="64007" checksum="2F71CEC46EC0D5B2" modified="2023-11-08" version="1">MTTFPNWRLGGERCDAMNDGRDVLTSLQSLVPTSPHGPHPQSFNSNLLPLDGFGMGGNGLFDFSGPLEAMFLSPNSWPSDPSLAVATSDLDPVPQPIYQLPFGHQSSGPLMETAPAATHRGAGSIQSLPDRGPHQPDPASTSPFSAENAPIGHVDEELLLNTFLQMLMPPILTPVEIGPKWASTRAFFGSMAAESPVVRSAIMAFAAMQMQRSGLGGDVMKTDWRPLYDNAARQLTGALAKKRKNEENENSQSGLKYSLASFFLLTYTDVRRSQSPRGFKLLTETLPRAHANLREAYTLIQNATKTTFSVPERRLISWLRLLDARAVSTAGGEGLFLADTDESLFDASPAANSGSEPETLDTEIEEILFDVLYHPGIVFYQKVQSFVGRITRIDPWHRSRGTVSDETEVMALAAQISKDLHALYGQRPALMDHAVAGNLTEKHLAKNLAAALTRSFRTYLANYYASFLHLQRVAHVIYPKTKDVTTAIVNIKRLAHLMADETLPVNLLWPLLMWGCEEDDLEERAWIVNAIRGLESIATNAKATADLLEEVQRRQDEGKRRVDVRSVSQEYFASHHFAIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM44</accession>
    <name>A0A9W9CM44_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Nucleoporin nup57</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">NUP57</name>
        <name evidence="6" type="ORF">N0V83_005539</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM44"/>
    <dbReference type="OrthoDB" id="6162375at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036228">
        <property type="term" value="P:protein localization to nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.3600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.490">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024864">
        <property type="entry name" value="Nup54/Nup57/Nup44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025712">
        <property type="entry name" value="Nup54_alpha-helical_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13000">
        <property type="entry name" value="NUCLEOPORIN P54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13000:SF0">
        <property type="entry name" value="NUCLEOPORIN P54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13874">
        <property type="entry name" value="Nup54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup54 alpha-helical" evidence="5">
        <location>
            <begin position="217"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="65"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="106"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="140"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4369775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="430" mass="48162" checksum="3F9FA348CD4B0741" modified="2023-11-08" version="1">MSFSFGNLGSGAGGAEKRTGTLFGSTTAPAGGLFGQSQQQQPQQQQANAGQSSLFGSTAAPQNNSLFGSTQQQQTGGSSLFGQPQQQQQQQQQQQQQNAGASLFGQPQQQQQQQQQAQQQQPNLSNSLFGASLQPAPPLNSVQAQSVQQQRTGLEQLRQSSAQGFAGSSTSGHREKSVVEQIRILNNKWDPANPECVFQHYFYNAVKPEEAPFYGPSQGEDERKWEEALSKKPSPGAIPVLARGFEQVAERIQQQAQAVVHLRTRLHEINNSLTMLKDAHELNVASRITEAKRKHVVFTQRTLALATKVQILRNRGYVMDQQEEDLKKKLFELEKKTFDPVLGGRQEEIWARMSGVRERARILQEETEKLGKSIENQQNGELLSGDDQKALEKLLKDYDRQLEHLKKWVDDTKDEYDEWETLKQARPAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CND1</accession>
    <name>A0A9W9CND1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_004831</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CND1"/>
    <dbReference type="OrthoDB" id="4524805at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040894486" evidence="3">
        <location>
            <begin position="24"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4371611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="415" mass="45669" checksum="5870BB4125729EBD" modified="2023-11-08" version="1">MLDRALPPALLSLILVISRSASAAPSPYNFLDNYRDPAPSPEDGPPASYHADRDPSRLGYDVGGVVGGYILTVLIWGVLLLTVGRRMRRKALDPPKQLELELQDKPTRLAIKTGPLSPPLSARSWLKKFKKTDSAVGSPQSPVVQSPGSFDQKVLDAHKDQAQADMERLYAAVMDHDERKHFSKVSVEEADMIGQERRRPSAISIARSHDDSPISPVKAIYPPGYHNGPVTAPLPRDNRLHEQQPPASPRSVLSKRSQVSQMSAATPTSSSSKNARFNLKNLRISGPVQKYPGADSDDEARTPLSPLLQRPRRPSSPPTQQNSPTTPYDPSEELDQCSTARTRMDKLSLTTPKTGVPFTPYSPYMPFTPITPVTPSHLVGRKERKMLSKIEGRRRRAAAGEDLVQSPKEIFGDAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNR8</accession>
    <name>A0A9W9CNR8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Alpha-1,6-mannosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MNN10</name>
        <name evidence="6" type="ORF">N0V83_004068</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNR8"/>
    <dbReference type="OrthoDB" id="407658at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000117">
        <property type="entry name" value="Glycosyltransferase family 34 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008630">
        <property type="entry name" value="Glyco_trans_34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306:SF5">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05637">
        <property type="entry name" value="Glyco_transf_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4372294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="447" mass="52518" checksum="25977213848A59D8" modified="2023-11-08" version="1">MSRSPSPRPGGWSSPGLNTPYDTGGQSSPFANGSSSHNVTWASAQARSAEVKGYNGFTPRNQGFFGKHFRRISTSLPFSYGEKEKLGRGRPQGNKVMQLLNRIGWTIWRLRRSLGLVLLIVLSIILFYVTPLHRMYRRSPLFGGGRKYVVILAANQGGGVMEWKGPREWAIERDSVKNKKKYTKTWGYDLEIVDMSTKKRYAHEWRESWEKVDTIRNAMRKYPHAEWFWWLDTNTFIMEPTYSLQKHIFKRLQEVTYRDINVYNPLNITHPLMDEYLDPETRSAVGDGKVDSINMLVPQDCGGFNLGSFMVRRSVWTDRLLDIWWDPVGYEQKHMEWEHKEQDAFEYMYQNQPWIRPHVAFIQQRQIMSFPPGACGDKGDDPNIHYQEKDRDFLVNMAGCEWGRDCWAEMYNYRQLSNRLNRNPWEKFKDGISDFFKSKKKDEKKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNZ8</accession>
    <name>A0A9W9CNZ8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNZ8"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="272"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="316" mass="34506" checksum="87FD0650486462A1" modified="2023-11-08" version="1">MAYHSTQYLNGGKDEEDALAEAISRSLTDNAAPFSTTRPVAPSSLRRYKGDKIKAKPISMIGHNELTRKDSSHHKPKPKPASASSSTAPHVSLEALHKNLGGAATFLEDSSHEELKVNLDTMAAKVRHWQRKVAQAMHALAVAETKRIEEIQDSLDIDRYIEAQHQAQLHHLNEKWEAELAKSMEEQMKEIDRLRHENAALRSPVMAREGESAERYSSGEDDAGVEMLQRERDGLPGQKTQGFWDATGGTRGKRPRQISGREGTEGRVQTVSLSPSPSPSNSGGVGGAGKRSLVHGGGEERVGDVGRSPKKAKNGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP08</accession>
    <name>A0A9W9CP08_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pre-mRNA-splicing factor 38B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002644</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP08"/>
    <dbReference type="OrthoDB" id="2431475at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40132">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 38B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40132:SF1">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 38B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="415" mass="48285" checksum="16388C2742F1D234" modified="2023-11-08" version="1">MASRIHDDEYVANLLKQDAKNATKKYELVGIDAFNPKRAKSGAPKPNTGFLRNLIRQTDSHNAALLAKEAEESNARLKSMNREKDKERRKDEDRKQKKAEGRLTPVLSDEDAYHRHSQGRSDYDRDGKRRGERRRERHRDDSRDARSERYKDRDEGSYRHKRRHEASDEDRSARKRGRHRSYHDQKHRHGHSSEEDEYSSRARERGERKHRRHRSYSRTTSRSRSRSPRAEKHARNHKHRDRSHDPVPDQRTFKPRRRPSDPTNAARKPRRRSASPVSDSDPLEAIVGPLPPSALPPVRARGRGAHKANAMGIESRFSSTYDPSVDVRPNSDAEDDWGDSVEAFRDRQKWKQQGAERLKAAGFTDAQVKKWEKGDEQNEEDVVWASRGQAREWDRGKIIDEDGDVALKADFGRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP74</accession>
    <name>A0A9W9CP74_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003341</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP74"/>
    <dbReference type="OrthoDB" id="64477at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43415:SF3">
        <property type="entry name" value="GNAT-FAMILY ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43415">
        <property type="entry name" value="SPERMIDINE N(1)-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="43"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="230" mass="26232" checksum="ECE435E3F059B948" modified="2023-11-08" version="1">MSQQESSFASKRLTFRAIRHPEDLAVFNAINADEKGYQNSNYSNIKLPTQANAEKFMKETADESLLGAIIWLKPEFQETADSPQKPAAPVQNSEEEILHPTWGTAIGEIHLSALPPAHAHHRWTDIGIDILPAYQGRGYGGEAIEWALRYAFVQAGLHRVRIRAFAWNEGAVRLYKRLGFKDEGREREAVWFMGRWWDAVTLGILEDEWRARERERSGYNNLVDVLVIDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP79</accession>
    <name>A0A9W9CP79_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003356</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP79"/>
    <dbReference type="OrthoDB" id="5407894at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="610" mass="66057" checksum="1880BE2A57928973" modified="2023-11-08" version="1">MTEPLAFHTGHFAGIQHPHAEARSSSIPATPEGAPCTDTAVPRGPADAPINDFSSLLDASAPFPLPLASPQPLQTPILEDRPHLDLSFAGIGLPSTNVSLPTPTVVKTAHNLRLPSFDVLGIAAPHPDHFSSRPHYPFSPLGAGPLSKPEDPLHALSPPLDFYHQADGAHDLPTASPKLARAQVGHIVPTWTPPQEPGTIVWGTFVNVRTAGLGSPPSSDPGVSPNLTLTASATAPGQAPIIVPTYAELSDAMRMAAWIEDVKNIISKRLLYSVASTWLIVLANESECLDLDSVKILSHALPCPSLTGHLFGRIIANIHDSTTSHTLWINVFHAVPGRYTLSDLPKSPPSTPGPAVGGDSYFTSKVFDSAVAIPDYQLDSKLLPPSPRPVVPPGSINVSVVERYIPPTNTNEFAEMFTFRGRSLLYDRLIELSSDNGILLFIYPTRTGGRTFMQQYLGPILDPLLRTLTVVHDLHSELGRSLGQMSAVNYLDEYEHLEAQLTKFIDALGTRQGNADKKDIYEIIHASQEEMVLGRAEWADDMWVKQEKPRVREAISKYFRKSKKLPTGELTGANLIQEVLEGVSKREYADGGPKKGIEVGVFIVKKTCSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPS1</accession>
    <name>A0A9W9CPS1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_002706</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPS1"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050364">
        <property type="entry name" value="Cytochrome_P450_fung"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46300:SF6">
        <property type="entry name" value="CYTOCHROME P450 2C30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46300">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="252"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4373967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="54513" checksum="099EBF7144CD6D43" modified="2023-11-08" version="1">MEGFTNNKQIPDVPTTNGTFSALESITSPMGHLPSSFIRTAIFIIFLLLTHRIIRYLTSELPPRGSNLKPLPGPLSTLPYIGRIHDVDRNHAWSALHKFSRQYNGLFSCTLGGETHIWVAREDVAQDLLCKNAAICSARADLGSYPGVTKGFKYLPLLGYTDAFLGQLGPSGPVMNLVPPLMHLPEWLVPGKREVRLRQEREAKLWEGLFDRSKDKYKKGGYPKTYVGASLESKDGSTEGKLLFENETEAKYAVGMLCTVGIFTIAGPAILFIMAMILHPEWQEKVRAQIDEVVGDEMIDLKHSPRLPILRAVIKECVRWKSTVPLGVPRLLSSDYTYDNYHFPAGAVVHVLDIALSQDPKRYQNPSTYDPDRWLNPTSRNFKAPLTEFPRLKGHHIFGRGKRACPGQDLAEAELFVFCGNLLKFFTLSPALDEKDEPIWPDPDKWTTNVIGGPLPFDCRIEVRDKKKLDMVEKMYSEAFEKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ03</accession>
    <name>A0A9W9CQ03_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MFS general substrate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_002391</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ03"/>
    <dbReference type="OrthoDB" id="4139357at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010573">
        <property type="entry name" value="MFS_Str1/Tri12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF109">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06609">
        <property type="entry name" value="TRI12"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4375305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="500" mass="53365" checksum="C5429749309CC828" modified="2023-11-08" version="1">MAAISMAMCYNAYLFTLLIPPAILAYINADLGPDPRYTWITISWNLGGAMFVTVGGRLSDLFGRRWFFIVGACIIILGSIVSATGQSINQMIAGGALFGCGSGFLEMAFGAVQLTMRRAIIKHTGNWRICYYIMIGFQVINLTLLVIFYNPPTFKEKKIEHGKSAGQLLREFDWLGMFLFIAGGTLFIFGVSWGGSLAPWISAVVLAPIIIGLLTLIALGFYEAYVPLAMPLFPPRLFRALRHFTVPILVMSIGSMQYYSNATLWPRLSQLIYATDEISKGLYSEALPLGTIIGGIIVAFSRSACVGAMSTASMDNPVKSIILTVIISTCTSVNLLNGMVLVGFGIVYQEDIGTAAGLAGTSRLLAGAIATAIFSNVTNGKYATSLPTAVRANLAPFNLSPAVVTKLIAAAKANTAAGYAAIPGITPAIKAAASLGNKQAYLEGAHLSYLVALAFGLVGVIAAFFIPSVDKRKYTEKTVALQKLDRRALEEKKLANVSGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR11</accession>
    <name>A0A9W9CR11_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="4">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">UBP15</name>
        <name evidence="12" type="ORF">N0V83_000320</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR11"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140492">
        <property type="term" value="F:metal-dependent deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02659">
        <property type="entry name" value="peptidase_C19C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000011">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000005">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024729">
        <property type="entry name" value="USP7_ICP0-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029346">
        <property type="entry name" value="USP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF644">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14533">
        <property type="entry name" value="USP7_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12436">
        <property type="entry name" value="USP7_ICP0_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 12" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="MATH" evidence="10">
        <location>
            <begin position="64"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="11">
        <location>
            <begin position="220"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4377495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1132" mass="130358" checksum="A4F82DEDDB2AA543" modified="2023-11-08" version="1">MDQIGSDMLVDDEYDEKQHVAIITPDTDDQMDEAEIEPEPRADEYDAFLKKHMPENPEYETEAEVHHTWEIKDWRTLTRREHGPVFECAGHPWRILFFPYGNNVDFASFYLEQGYEEKQMPEDWYACVQFMLVLWNPKDPSMYLTHTAHHRFTAEEGDWGFTRFAELRRLFSNSWEDRGRPMVEDNEANVTAYIRVLKDPTGVLWHNFINYDSKKETGMVGLKNQGATCYLNSLLQSLFFTNAFRQAVYQIPTAEESDRSNSAYALQRLFYLLQTQSSAVGTTDLTQSFGWDSKQIFEQQDVQELSRVLMDKLDEKMKGTEAEGALTNMFVGKMKTYISCINVDYESSRIEDFWDIQLNVSGNKNLDDSFKDYIQVETMDGENKYFAEGFGLQDAKKGVIFESFPPVLHLQLKRFEYDFQRDAMMKVNDRYEFPEIWNAAPYLSEGADRSESWIYHLHGVLVHSGDLNAGHYYAFLKPTKDGHYYKFDDDRVTRATLREALEENFGGDYAQANGNAGQRNPYTRAWSAKRSMSAYMLVYIRETKLNDVLMDGKSVEPPKHLAERLAEERAAFERRKKEREEAHLYMDVAVASDKQFKVYQGFDIVPWKDTSDPATAPNIYKVLRATTMADFAKTVAEDLGVEADMLRPWSMVNRQNGTVRPDTALEFPDMTVEEAANKHGTKQAQFRLWIEKAEKRDANGAPVFGETLVDLKGAQNNRPLMIFLKHFDTTTQSLYGIGTFYAAIQDKVSDLSPTINNMLGWPAGTQIKLSEEIKQNMIEGMKPKVTLATSEIQDGDILTVQRTLSDKETNQITTAGGYVEAKDFYDYLLNKISVEFVPRLAEATELPSFFLTLSKKMAYDQFASKVAEHLKVDPSHIRFTTVSTAGKPKAPVKYNPNSTLNNILFPGPYNYSTTATQRPDAMFYEVLDMSLKELEQRKPIKVTWLPEGLSKEEEYTLMVPKNAQVSELLEALQKKANIDDETMQKVRAYEAHMHKFHKSLPPDHAIMSLYDYTQVYVAPFPEDESPKRIMVFHFDKEPSKAHGVPFQFSLKEGEPFSDTKQRLSDFTKIKGKQLDKIKFALVSRPQYSRPEYIDDDEVLWDIVGTRDDIALGLDHTNKSRSFWGKSDSIFIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRH2</accession>
    <name>A0A9W9CRH2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">F-actin-capping protein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CAP1</name>
        <name evidence="7" type="ORF">N0V83_000835</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the F-actin-capping protein alpha subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRH2"/>
    <dbReference type="OrthoDB" id="340550at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008290">
        <property type="term" value="C:F-actin capping protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.210:FF:000003">
        <property type="entry name" value="F-actin-capping protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.60">
        <property type="entry name" value="F-actin capping protein, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.210">
        <property type="entry name" value="F-actin capping protein, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002189">
        <property type="entry name" value="CapZ_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037282">
        <property type="entry name" value="CapZ_alpha/beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042276">
        <property type="entry name" value="CapZ_alpha/beta_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042489">
        <property type="entry name" value="CapZ_alpha_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017865">
        <property type="entry name" value="F-actin_cap_asu_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10653">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10653:SF0">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01267">
        <property type="entry name" value="F-actin_cap_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00191">
        <property type="entry name" value="FACTINCAPA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90096">
        <property type="entry name" value="Subunits of heterodimeric actin filament capping protein Capz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00748">
        <property type="entry name" value="F_ACTIN_CAPPING_A_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00749">
        <property type="entry name" value="F_ACTIN_CAPPING_A_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0117">Actin capping</keyword>
    <keyword evidence="4 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4378005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="270" mass="29552" checksum="DAB073F44EDAF356" modified="2023-11-08" version="1">MSRTAALSAFIESAPPGELADVTKAVKSILGDDAVQNELSPAFEKYNEEQFTTTKLPGGSTEVLVSAHNSLGDGRYYDVETQSSFDFDHATGKASAVQSYVLESQHEDLVKSLVKSLSTHVSEHYPKASYGVFPTSDDTALAILTVANKYSPTNYWNGRWRSSYIYSPSSGSLTGTIKVDVHYYEDGNVRLLTNKDVSLSVGGSATASEIIRKVAAEEKKYQEDLNKAFGSLSEGAFKALRRQLPITRQKIEWEKISGYRLGQDIGGGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRT2</accession>
    <name>A0A9W9CRT2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7137 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_000233</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRT2"/>
    <dbReference type="OrthoDB" id="2435509at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055561">
        <property type="entry name" value="DUF7137"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42028">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42028:SF1">
        <property type="entry name" value="YALI0E30657P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23585">
        <property type="entry name" value="DUF7137"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040870242" description="DUF7137 domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7137" evidence="3">
        <location>
            <begin position="109"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4377408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="278" mass="29470" checksum="E7EFED3DF137BEED" modified="2023-11-08" version="1">MRPSQVLAAVLAMSSVSVAISDAFDNIHGLAEVKDVLFGRQDDNSDNASSDAPKSTQAPKSSDAPSTTKNDDKASSTGKAASGSAKASGSQGTKVTTSKSHETSYDPRLPAGGLSMVTPNALAGSQFYKVGDWVTFAWNYTSLSQTPSAIDVLASCTANQATYTIAVNISAEQTQVLWDTKNTPDGQAPFLTEKYTLMIYDADLSVSATARAGYLGAYTGFSFGMYTPQPYTPWDNFTCANCNGALSQFEKMTIKAILMTTGTTLGSLLYFTYAFGLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CS70</accession>
    <name>A0A9W9CS70_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Holocytochrome c-type synthase</fullName>
            <ecNumber evidence="10">4.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CYC3</name>
        <name evidence="12" type="ORF">N0V83_000658</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Lyase that catalyzes the covalent linking of the heme group to the cytochrome C apoprotein to produce the mature functional cytochrome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>holo-[cytochrome c] = apo-[cytochrome c] + heme b</text>
            <dbReference type="Rhea" id="RHEA:22648"/>
            <dbReference type="Rhea" id="RHEA-COMP:10725"/>
            <dbReference type="Rhea" id="RHEA-COMP:10726"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:83739"/>
            <dbReference type="EC" id="4.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cytochrome c-type heme lyase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.17" evidence="10"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CS70"/>
    <dbReference type="OrthoDB" id="1158011at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004408">
        <property type="term" value="F:holocytochrome-c synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000511">
        <property type="entry name" value="Holocyt_c/c1_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12743">
        <property type="entry name" value="CYTOCHROME C1 HEME LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12743:SF3">
        <property type="entry name" value="HOLOCYTOCHROME-C SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01265">
        <property type="entry name" value="Cyto_heme_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00822">
        <property type="entry name" value="CYTO_HEME_LYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0349">Heme</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="303"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="37"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4377828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="328" mass="36971" checksum="A8AAA99B528213ED" modified="2023-11-08" version="1">MPRGNAEPPPSCPMHKKATPPVVAEKVPQGACPYVPPNASETPSSTTTPPKTGLLSRLNPLNNMPLELSNKRAEAQKQDLPLDRERSTIPKGDGSLWEYPSPQQMYNAMLRKGYTDTPIDAVESMVGVHNFLNEGAWAEIMGWERRFSRGIAEGYRICAKGEDLANLALGTAEDPFDTTTWNQADIPPPKLLRFTGRPDEPTPKSQIMQWLGRVMPETYGTAPPFDRHDWYVQRCNEKGCKEVRYVIDYYEGDPEATGEPVFFLDVRPAIDGPTGAAERAVRWGTDVWWRASGGVARELRKLEEAKKRKEEEKKRKEEELNAKSRQYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSN5</accession>
    <name>A0A9W9CSN5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000992</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSN5"/>
    <dbReference type="OrthoDB" id="5555409at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4378159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="94" mass="10105" checksum="B9422A2EEF615CD4" modified="2023-11-08" version="1">MAPMGSKAKAPTDAIYGSVSTADLVATIKGALAHNDEAARVMLNESDVRFVSGHEEGDASRVKQLGVFKVEIRLPGTEEPLIRNIRIRAKETEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XX68</accession>
    <name>A0A9W8XX68_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010938</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XX68"/>
    <dbReference type="OrthoDB" id="3686314at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="198"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="293" mass="33649" checksum="2080CE61574511A7" modified="2023-11-08" version="1">MARRPIGVRGLPFKQREALSKGYPVHFRKADGTFFEVTMPLPLFKATTSDKDLLVTTRNGVHQVTVPDHTNPAALKFFITHMNNLAAEQYVGEVPFTKHMALNLHLCSVAEDLGMAKYTQYIFNGYYHRLKTEVPSRDDVESIGQVDTPLGNRLFNTVGHRLAVLAWDGEHPNHAAFEEYLTKNTRLATIVHDLFAKREGAIERQARLEERQARFEQRRLEQEEADQLAAEREARMMAVEKERLAKDKKKWNAQKQKDADIRARVVEKKRAGEKLTREEAAMHYAMYGKHVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXK2</accession>
    <name>A0A9W8XXK2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD40 repeat-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_010422</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXK2"/>
    <dbReference type="OrthoDB" id="1932312at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040324">
        <property type="entry name" value="WDR44/Dgr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14221">
        <property type="entry name" value="WD REPEAT DOMAIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14221:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="221"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="294"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="336"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="199"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="649"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="847"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="696"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="748"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="784"/>
            <end position="797"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4362329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="911" mass="99369" checksum="B820FDA73B0CDA58" modified="2023-11-08" version="1">MVDTIPKIKVTDPQVKSANPFEKQNGYSATIEAAGPLTNKKLGAKLRNPASPSAQSNETHNRSSTDGRRPSEAAIDPLSQNTDPVYTQSPTSPSEANADKKQSGETQREIGGASKADKKKGSSFLSRFIGANKKKGILDGASDNGSEADDLRREGMDAQLYVENYSFSPKVPQPPSYIKVRTKFKTKKEFDRVFLAQELRSGSDKKSPPAAGSNPAPQSGSAATHNPVWSVEFSKDGKYLAAGGQDRVVRVWAVIGSPEERQSHEKDEDDPQAADNGAKHLSAPVFQQKPFREYHGHTSTILDLSWSKIKNNFLLSSSMDKTVRLWHVSRDENLCTFKHSDFVPSIQFHPTDDRFFLAGSLDAKLRLWSITDKSVAYSITVPDMITAVCFTPDGKTCIAGTLGGLCMFYDTEGLKWQSQVHVKSTRGQNAKGSKITGIQATHWPSGSGNGEVKLLISSNDSRVRVYNMKDKALEMKFRGHENNCSQIRATFADSSGHIICGSEDRKAYIWSTTAAEGEKRNQRPMEMFEAHNSITTCTIIAPVQTKQLLSASEDPIYDLCNPPPVTLVSRAESVISSRAPTEAGSALPTPAPNDTTFKRAAESPAYIARSTHLGGNIIVTADYTGSLKVFRQDCAFAKRLRMSETWETASLKRGGSKIGRPSSILSRTSRSRRDSVSTQPPNDRIMTWRQGISHGSFDSTNSSLPRRSASPRKSLAGLSFHSARTPDSPAMGPTQLQDTVNARPIGTPKTSVSMDRTTSGRSVSQSENSASPHKQPQPQPTPPAAAAAADPKPYDTYNRQPSASNPLAVYNGQSWLFWSTRDKSSLTASNTHNENNMNLSPRISNASQISRLTSEASDRGHSAEEEEDADGDGDGRDDGVKCRHCGGEEFKVRFQEGEKVLVCRDCARVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY75</accession>
    <name>A0A9W8XY75_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11 12">Endo-1,4-beta-xylanase</fullName>
            <ecNumber evidence="4 11 12">3.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">N0V83_010354</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11 12">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-xylosidic linkages in xylans.</text>
            <dbReference type="EC" id="3.2.1.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11 12">Glycan degradation; xylan degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11 12">Belongs to the glycosyl hydrolase 11 (cellulase G) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.8" evidence="4 11 12"/>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY75"/>
    <dbReference type="OrthoDB" id="2115822at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031176">
        <property type="term" value="F:endo-1,4-beta-xylanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013319">
        <property type="entry name" value="GH11/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033123">
        <property type="entry name" value="GH11_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001137">
        <property type="entry name" value="Glyco_hydro_11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46828:SF4">
        <property type="entry name" value="ENDO-1,4-BETA-XYLANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46828">
        <property type="entry name" value="ENDO-1,4-BETA-XYLANASE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00457">
        <property type="entry name" value="Glyco_hydro_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00911">
        <property type="entry name" value="GLHYDRLASE11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51761">
        <property type="entry name" value="GH11_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9 11 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 11 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 11 12" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11 12" id="KW-0858">Xylan degradation</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040896161" description="Endo-1,4-beta-xylanase" evidence="13">
        <location>
            <begin position="20"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="GH11" evidence="14">
        <location>
            <begin position="33"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4362261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="224" mass="23880" checksum="5688E948FDDF1B48" modified="2023-11-08" version="1">MVAFSHLAVALSAVAGSLAAPTANPVEDLPDFELTNVTRLMRRQDYNQNYKTSGNVNFSPSSTGYSVSFSNAGDFVVGKGWNTGTTRNINFDGATTASGGTVLVSVYGWTKSPLVEYYIQEYTSNGAGSAQGTKMGTVTCDGSVYDIWKHTQTNQPSIVGTTTFTQYISNRQDKRPGSGTVTTKCHFDAWAKLGMNLGAHNYQTLSTEGWGNAAGNSKYTVSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYS9</accession>
    <name>A0A9W8XYS9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009699</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYS9"/>
    <dbReference type="OrthoDB" id="202203at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735:SF24">
        <property type="entry name" value="NUCLEOTIDE-DISULPHIDE OXIDOREDUCTASE AMID-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17180)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="60"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="451" mass="48919" checksum="07DDA3DC2A8AEE91" modified="2023-11-08" version="1">MRLQGITDTLGNLTMSNPHRVLVVGCAYGGISTIVNLLECSRGKPRESYYPGPDFKGARSRRGVEITVIDERDGYFHSVGAPLAHVAPKYTNYMWKRYSHLNELKHPNLHFKHGSVRNINPEAKVAEWCDRNGKTQQQAYDYLVMATGLKRHWPAVPKSGSYEEYLRDGKAFIEKITGGDPSKHAGRRVVIIGAGAVGIEFAGEIKNYYPQIGVTLVHSRAEVLSSEPLPSDVKARAKLLLEEEGVDLILGSRASITELPNGQFTVTLANGETITADFVIDSTKKGTPTTHILPAECLNADKEVLVTQSLMFKPDTIPNASSHFAVGDVIAWTGIKRAGSATVMGQTAAQNLYASILNSELSPEAEPYSTAELPHWDAVIGLAVGKQCLTYDPKSGIKYGVEVMKGYFGDDLGWAASLKYMGLTDVVEKVEGGPEKATMVEVGVKPVSAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-06-18" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYU2</accession>
    <name>A0A9W8XYU2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 20">Serine/threonine-protein kinase Tel1</fullName>
            <ecNumber evidence="5 20">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">TEL1</name>
        <name evidence="25" type="ORF">N0V83_010575</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="25">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17 20">Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates histone H2A to form H2AS128ph (gamma-H2A) at sites of DNA damage, involved in the regulation of DNA damage response mechanism. Required for the control of telomere length and genome stability.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Associates with DNA double-strand breaks.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Chromosome</location>
            <location evidence="2 20">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 20">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the PI3/PI4-kinase family. ATM subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5 20"/>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="381190at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05171">
        <property type="entry name" value="PIKKc_ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000034">
        <property type="entry name" value="Serine/threonine-protein kinase Tel1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000019">
        <property type="entry name" value="Serine/threonine-protein kinase Tel1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038980">
        <property type="entry name" value="ATM_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044107">
        <property type="entry name" value="PIKKc_ATM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021668">
        <property type="entry name" value="TAN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11640">
        <property type="entry name" value="TAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01342">
        <property type="entry name" value="TAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14 20" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7 20" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11 20" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 20 25" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16 20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="15 20" id="KW-0779">Telomere</keyword>
    <keyword evidence="9 20 25" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="23">
        <location>
            <begin position="1875"/>
            <end position="2481"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="22">
        <location>
            <begin position="2595"/>
            <end position="2904"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="24">
        <location>
            <begin position="2948"/>
            <end position="2980"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="2872"/>
            <end position="2936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="2878"/>
            <end position="2895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="2917"/>
            <end position="2931"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU365027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAJ4362481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="2980" mass="335172" checksum="ED1B9D6D557FA992" modified="2023-11-08" version="1">MGATNIQDAKLLIQSSRVVDRTTGLKDLVHILKHNRGKPSLEALGNKAYLALCETLFQCLRDERSSFLRSKPKPKSKSKSTSTKTGALLPLSASALRHVIASGVRTIKSSTVEIIIDTIIEVLPGQDGLIKPLLEDLPKTLRSLLEYQPHVERLSKDCWDAAVDFCIESLSGTFTEPEVEPQSSWSTNVSSRARTPLEVTDITSSRASPRMPVTRTKSAPDEYAHSIEDFVHCLHLLVKASNAPVLDKAEAILGALLQFLQQRTGRGSVAAAALAAVNSVLVHTTLQSLELTKSTVQELLPLMKSMWSELILRDEIMITLMHTEAHILSLLADPDDEATHSDLEALVETIYNDYRRRQETTAHQYLEEDHLCFRHLGRADKNTHPLNTCAFSMETEYQRFESLWATVSTIARFSFMLDDRRQTITHDRSDTEESISKRLRVTQLFPEYLRHVFEPRSNAKRAALQVVAFMVQEGRYDEDDLQSMLEKLISCISDENPVHSVWAMIGLAGAALQQSATHAALQPYWISAWQSASRAVTSISNSRAACHLMDVLLRLNIVPFSSVSGTVQSMLLSIELSGPALLTETSSSLITTLIRERIKENPTHFNSTAERILNWLLSKWTPSQWSERTYSSLNAHHCNARDVLRVLYACLDRPFSTTPPAPFLMLGPIAQARTRNVRYKDLAQYLLLLDIKEEYLAQAEVSQVSPTEGPSEHHPTQMENRVLDFCLSEVDKTMQRWKDVSSANSTNITSDMMRVVTNLCIVAPAVAALGAPDDRRVSALESSATSLSESFVDNLAKPQTEQYKIDAVLETCTKSLPDISYLPHLNSQVFKQAGVCVLSQHLSKVLWNRRDVKQSFYAENDDFMDVDEDVDSQMTVGQVGFEVDVPRHDVQAENDASALRASCSAYLHLVSSIANNSETEHFDLPSKFVDHLIALHESELLRSRQFLRALWDSELKISRNDCLNLLERLSEALIDPRAREYNTSEVANGMLVEVLIGTALVWAPDATDREGQDLYDNIEALYAYYVREMEKGGVRRSANLQKIIAAFLQGLLKYHPDFGQNRKVPSVRTSLFELLSRGEMTVKYHIAERLPQMFEDFVLSEHDKILQDVDSSLPGDDEGVEGIAVRLLVLSKLAAQWHTLLRHSIYRIFATAGAVPGAAQHAKRCIFNVAQARGVGDSQCLFRLFAPQIIFTWLDRGRAVADIPFLTFGYTALLDLLRDIEAEAVGQAMMFGRKGEVEYLASQLGTTISGILSKNIGKAAAYTIAWDTCKGFARNKAEPSFANLLKEMIGADKYYDLVQKHFPQVLAHIFQTIDLEERISKSLDKKPPFNAAAKLIMEMTNISHSAQGLNIGIEPSFSAFYLPDQLERLCRRTADNPASFWTPSNYTFVMRSLLDRIHPALGSLFARSMIRKIRIVVALAGPVAYEGYPLQMTLQSLRPFLTDVQCAEDTVGIMQYLFEHGSYYLRQHLSFVTGIGLSILISVRVFLGSSQDSTTQQSQHVATMNAANRFHSWLTEYLKNHAQAITTTERSSAVKAFKLITTAASQVNAEGNSIRGSEESKLLLEILDDLKSGRKLLNKTSREVALDLLCQNFQVAPSARDDVLGQDDDASEYAPLVWESCRRSNVGDGYLLWTARVLGRAFNACGEVKKSTMHSLPWSFSGHVSKDSLGRISREAIVREVIDLFYSDDRNEVSLAEDVVRRLVFRLSSADQRYIDEMHRAIPEAIGKALGLYMPQDTDLALPLFTEGLEQAATPTESKPVAIWIRDLAIALCRRSADDPILGALPRFLVGIDHMARRLLPYILHLVLLEEFEGNRDVRNIMSEATMAWFRDCDVSTAPHVRVIIQCILYLRSQPVPKEVTRVDRDRWLEVDFLRASQAANACGMYRSALLFAETSSGQPIVKSSTRRSSVLVDPPRIPLELQLSIYKNLDEPDSFYGVDRGSTLLSVLDRLDYEGDGVKSLLFRGARLDSQMRRLNEFEAPDSRGTVKSLIMLNMNSVTHSLLSNDQFRDIGDDAVESTLHTARKLGQWDIKAPEMNHTEPSTLFKAFQGLYYAKSAAEAQGNFDRQLLATMSFLSGKNSSSVPTKVRLRTLGALTEADEVIRAERSEHLLDTWDRMKAREKWMRAGEFNDVRQLLSCRETLFNVLSSNTVLVDSLHTRTATVRGMEVEALVSSSAVCRKHGALQESLATVTYLSDIVPECKSVGLDIDATAQHEVANVLWEQGETEISIRMRQHLIDHADFDSQNIDLSLPVLLARLGHHLAEARLAKPDTIMRDYLEPAIRELKGQKQGSGPGQVFHEFALFCDKQLQSPEAAEDMERIKTVMDRKLQEYHEFTKLSKTDKSKGMRDTYYRSARRAKTWYDLDHAEYERMRKGREQFLRQCLENYLLSLFASDEYNNDALRVFSLWLEYSDTDLANAAVQTYLKDVPSGKFALLMNQLSSRLQAEDNDFQQLLMALVFRICVEHPYHGMHQIFAIQMKVGAITREDAVRAKDESARSRQKAAIGLANALSSDKRARSYWSSIFQSNEIYHHLAMFKGEKESTQQGRELPLDRYKESKDLVSKIPKLNLPPATLQIEVRPNMNYTDLPRIHSFKPTMSIANGLSAPKVITAKGTDGRPYKQLFKSGNDDLRQDAIMEQVFDQVSRLLKNHTATRIRNLGIRTYKVLPLSTRSGLMEFVQNTIPLHLWVMPAHEKYYPNDYKPDRCRKEIGTCQQDSLTTRVKVWQKIADNFHPVLRYFLLERFEDPDEWFERRLAYTRSTAAISILGHVLGLGDRHCHNILLDEKSGEIVHIDLGVSFEAGRVLPVPEVVPFRLTRDMVDAMGYTKTEGVFRRCCEFTMDTLREERESIMTLLNVLRYDPLVNWSVTPTKAKRMQEGNQDTAAQNGTARSTTVGPGGTPAPSGVPVGSGQVAGEEVVHESNKKREKEEQAGEAGRALSVVEKKLSKTLSTKATVNELIQQATDERNLAVLYMGWASYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZR0</accession>
    <name>A0A9W8XZR0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009657</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZR0"/>
    <dbReference type="OrthoDB" id="3509362at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF7">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="18"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4363365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="468" mass="52668" checksum="DFA6F60147E34A41" modified="2023-11-08" version="1">MAEPKPRQRKFAPRSRTGCLTCRTRRKRCDSRQPQCANCTRLNLTCEWQAPRKIVGEQSREPSTSSSPELQLAVPLRAALDPWDALPSDEASDRKLLLQYYIEAFVPSVSVASTPTSFYTSLYMPWAFQVDGMLDAILAITSGQLARRATSEDRAQHLRELSSRHEMKCYSFIKERVSPTGGLPRDTYQVAAIILILVGLEALNGVKTTRWISQLKSVQRILNQLSLEQSIVDCVEVDSLHRHFTYHFASASLMAKVSNASSTYSIEQDLLPLSSTLMSVTIDPLMGISYQLCDLISRIQYVTSSNPAFPHVTEASFNSIESGIQSWTYDNPFALGVDLPIALDLIALAEAYRLAALIQLYRSSPMHTSLIPACASRAMEFISRIPPGSSAESSLLYPIFLAGAELDEEAAIERCFKRLEEIQKRNRYENVSSVQKVLKEVWRPKLEGGQRRDWEDVLKEWDWSFTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZT5</accession>
    <name>A0A9W8XZT5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing]</fullName>
            <ecNumber evidence="3">1.3.1.124</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SPS19</name>
        <name evidence="6" type="ORF">N0V83_009830</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a (2E,4E)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:45912"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85101"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a (2E,4Z)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:61892"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85099"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.124" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZT5"/>
    <dbReference type="OrthoDB" id="2136131at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008670">
        <property type="term" value="F:2,4-dienoyl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045017">
        <property type="entry name" value="DECR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43296">
        <property type="entry name" value="PEROXISOMAL 2,4-DIENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43296:SF2">
        <property type="entry name" value="PEROXISOMAL 2,4-DIENOYL-COA REDUCTASE [(3E)-ENOYL-COA-PRODUCING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00026117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4363535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="313" mass="32674" checksum="6139FB8686AA5CD0" modified="2023-11-08" version="1">MPMDRYEYVSDVWRDGIFDNKVVFCTGGAGSICSAQVRAMVALGANACIIGRNVEKTETMAKDIATARSGAKVIGIGAVDVRRPELLQAAADRCAKELGSIDFVIAGAAGNFLATMDQISPNALKSVIDIDVLGSYNTVKATLPYLVESAAKHRTDGKTRPANGTGGRIIFVSATLHYSGTALQSHVSVAKAGVDAMSVSVAIEQGPKGITSNVIAPGPIADTEGVARLSKKETRVKAYKAVPTGRYGSVKEIADATIYLFSDSGNYVNGETLVVDGGHWHTAGTSSHGFEYPDFLLSGETVTGVAGGKKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZU4</accession>
    <name>A0A9W8XZU4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009703</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZU4"/>
    <dbReference type="OrthoDB" id="3557394at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF73">
        <property type="entry name" value="HET DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G01020)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26639">
        <property type="entry name" value="Het-6_barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="2">
        <location>
            <begin position="44"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="577"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="626" mass="71054" checksum="57D12149604B3083" modified="2023-11-08" version="1">MMASPEVQRPTNRDIRVLDLQPGSRNDDLKCDIRTVSLDAKPDFETLSYVWGQAPARDSIEVSHQRMLITPNLYSALRRLRYEDKRRTVWIDQLCINQWDAEDKANQVSLMRDIYRVCSHCVMWLGEIPDSTHGFDHEDAEAVFEFIRYVAEMDLEQPSDDNTTTTTTAPAPPSSDHHHHLDDEDLPTLFQNTPSGTTARKAFAAFAMYGNPWWRRIWTIQEAILPHTASFHWGPLSIARSSIILAAQRLKNGSRGFLAAFSPLFQARRRQHDELLRWLLYPVHGILHSWNGEVPLDLFMRWRHRQATDARDKVYALLGLLPAKVLPSAQACSYALSVPELFAQVTTDLMRYEGGLRPLVAASEMVHVTEELPSWAIDFACSNRVGKRQLKWWGHSHRYRQFSACGKHKLRFKVADEGRALYLTGVLVDRVLKVNSVYTVADDEPLIRGRVWDIISSAEGLLELFIASRPDLPSYPGGGSWDSALWRTMLGDLIMDEFPIDSARDEHEKHFKRARDRLAQGKPEVPNIVLESLRGMIPNHAFFITEKGYIGIGPPQTSAGDQVWIFYGGQVPFVMRKGEEDGDRGGGSGNDDQDRTLTLVGDTYVHGIMDGEAVKHGYAVQTVRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0G9</accession>
    <name>A0A9W8Y0G9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">precorrin-2 dehydrogenase</fullName>
            <ecNumber evidence="2">1.3.1.76</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MET8</name>
        <name evidence="9" type="ORF">N0V83_009219</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>precorrin-2 + NAD(+) = sirohydrochlorin + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15613"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:58827"/>
            <dbReference type="EC" id="1.3.1.76"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.76" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0G9"/>
    <dbReference type="OrthoDB" id="1721126at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043115">
        <property type="term" value="F:precorrin-2 dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3280.10">
        <property type="entry name" value="Siroheme synthase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028161">
        <property type="entry name" value="Met8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028162">
        <property type="entry name" value="Met8_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028281">
        <property type="entry name" value="Sirohaem_synthase_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006367">
        <property type="entry name" value="Sirohaem_synthase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01470">
        <property type="entry name" value="cysG_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330">
        <property type="entry name" value="SIROHEME BIOSYNTHESIS PROTEIN MET8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330:SF1">
        <property type="entry name" value="SIROHEME BIOSYNTHESIS PROTEIN MET8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13241">
        <property type="entry name" value="NAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14823">
        <property type="entry name" value="Sirohm_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14824">
        <property type="entry name" value="Sirohm_synth_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75615">
        <property type="entry name" value="Siroheme synthase middle domains-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Siroheme synthase central" evidence="8">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme biosynthesis protein Met8 C-terminal" evidence="7">
        <location>
            <begin position="160"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4364623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="247" mass="27275" checksum="DAC856606114A962" modified="2023-11-08" version="1">MKGPYPEIQGGGSLILAWQIRNKRVLVVGGGEVAAGRIVNVLNADAKITVVSPREGLNDEVAYRIEQKQVDYVDRKFEPSDLDGVDMVLTAVDDPEASSQIYKLCKEKRIAANIADVPPECDFYFGSVHRDGPLQIMVSTNGNGPKLANIVRRQIAAGLPHNIGAAVQRVGMLRKKLRKVAPNIDEGPKRMQWMSRVCEQYSLDDLCSMTEEDMEMLLGFYKPGTVPSLEEVRLQEADGAYDGPFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1T8</accession>
    <name>A0A9W8Y1T8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009932</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1T8"/>
    <dbReference type="OrthoDB" id="3799742at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="286" mass="29890" checksum="F937AEF5B7FD11D0" modified="2023-11-08" version="1">MFLSVKERARQIASGQFQPSQAAVEPPPPVVASKVVPAKVVTAKFSPSKGPGGSSKTAPSPSTPKKESPKASISKAPKTPSTPTSPKPSSVPSWKATAPKTPSSKTPSSKETKEKSSKSSSARASSPKASSSKSSSSKSSSSKRSSSPKTSPKIAAPAPPKPKLTRSKTSPPDLGIGIGSFADDPPEDHTTISGLKAACLKQASGVTGLGGRRLAVNLKQTSSGEWYAALKDLSSDKYLKMWMDRSKTYETQIEALEAYLVFAKKMKQGCRLFPPMSPKAASTKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1W6</accession>
    <name>A0A9W8Y1W6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_009978</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1W6"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000064">
        <property type="entry name" value="MFS allantoate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000295">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.7, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF59">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G06550)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="330"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="360"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="390"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="453"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="64"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4363681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="538" mass="59206" checksum="FA7FE32CB396B34D" modified="2023-11-08" version="1">MSKSESAPPATDVSKANIETSETIKPTQETRGKDKAAELLASNERIVVTSAENKAVLRKIDLVILPILLSVYFLQSLDKTTLSYASVFGLIQDAGLDPNSQQYSWLGSIVYIAQLVMQPIVAVLLVKLRIGKFMGVMVFTWGCILCGMAGAKNFAGLMATRFLLGAFEAAVAPAFIAVVQMWYKRGEQTNRNAAWYAMLGIVNILGSLLSYGLGHINSDRLHSYQIIFLFCGLLTVIVSVFVYIFMPDSPMEAKFLNEHEKLIAVERLRMNQMGVASRVWKWDHVFEAFMDPKTWLWFSMLTAVSIPSGGITTFGPLIIQSFGFGKFATILFNMPFGAVQIVATIGGAWLATWLKKKSPVLILLCIPPIIGIIILMVVGRGKEHRGVLLFGYYMTSFYPGISPLIYSWSGQNTGGDTKRKVTTSILFVGASAGNIIGPHLFKPSEKPHYYKGLRANLALFIAIVLLVVLAVCWIKILNRKHAATRESMGKSAIVVDTSMGSSKHTDAEEIEDALASGVGDKAFDDVTDMKNEDFIYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y221</accession>
    <name>A0A9W8Y221_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">chitinase</fullName>
            <ecNumber evidence="4">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CHT4_2</name>
        <name evidence="13" type="ORF">N0V83_008450</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 18 family. Chitinase class V subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y221"/>
    <dbReference type="OrthoDB" id="76388at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06548">
        <property type="entry name" value="GH18_chitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000005">
        <property type="entry name" value="Endochitinase B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000075">
        <property type="entry name" value="Sporulation-specific chitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF317">
        <property type="entry name" value="CHITINASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="9 11 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 11 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="GH18" evidence="12">
        <location>
            <begin position="66"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4365829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="468" mass="51093" checksum="269C926EE5EA5A61" modified="2023-11-08" version="1">MVDSSVVWLSRVGNGVSTASFQDQAYTVATPTTSNAIVTVTPTDSTPIATSAPSEAAADVETRSGYRAVAYYGNWDIYARKYFPQQVPASKLTHLLYSFADNKPDGTVYLTDTYADTDIHYAGDSWSDSGNNVYGAMKQLQLLKQQNRNLKVLLSIGGWTYTNTKKDMDTPAFTAEGRQTFAASCVDMVKNYGFDGIDIDWEYPQDTTQGSQFLALLTEIRKELDAYAETLVYGDDSGNEEKPHFLMSIAAPAGEINYRNLPLQDISDVLDFVNLMAYDYSGSWNNVTGHAANLYASISNPDSTPYNTYSVVNAYLAAGVPSDKLNLGMPLYGRAFTKTQGLGKPYDGIGAGTWEAGVYDFKDLPLAGATEYYNKEAGATYSYDSSIGMLVSYDTVSMAMRKVDYIKQNNLGGAMWWEVSGDRTDDKSIISNVVKGLGGSDGSGIESVSNWLEYPDSDFDNIKNMSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2P8</accession>
    <name>A0A9W8Y2P8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">HAUS augmin-like complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_008240</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAUS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2P8"/>
    <dbReference type="OrthoDB" id="5372507at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070652">
        <property type="term" value="C:HAUS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026243">
        <property type="entry name" value="HAUS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31570">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31570:SF1">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25762">
        <property type="entry name" value="HAUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="47"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4365620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="33975" checksum="B30865E2D244A0A9" modified="2023-11-08" version="1">MDNLTPSDLFSPSKARQQRAQAQDWAHIDSWLSYKYSGRTVPTFERNEETLKVLRELSMANERADEERNVLERVEREALKELDEVEKTEDDERILSQLKSSLTQEGEEALHAIASTAVILNTPSVDPETLAHALIQHTTTSQNLTNQLAHIQTLQTYLTKQISLLRTQLNELQTNSAFSTPPLLQRQTTEQTRQTKHLRTKIREYEDKLSSLQSSQNRSMTPGSRNIGSAEAIGEMLEQQKVLDELRERVVGLEAQVKEFAGLPADREMARKEVGKLEIRLDEVRRRRDELFEGLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2R4</accession>
    <name>A0A9W8Y2R4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_009458</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2R4"/>
    <dbReference type="OrthoDB" id="3266505at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF6">
        <property type="entry name" value="PROLINE UTILIZATION TRANS-ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="5">
        <location>
            <begin position="364"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="663"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="663"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="680"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4364003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="725" mass="81227" checksum="5002A2164E1B23A9" modified="2023-11-08" version="1">MPTTPFPNNPQIRPWDVFLPPSQIYSLYLGFLPKDLADEWFIYSEGPDNAGKLKVHFHRTWTGRKMAELFVVIDVKGEGAGKIVGLKWNGSSETNRMNEKEAKYMIRMSYKTKLERLQKRYAALEKLLYATLPDAEDLIRESDQGEPSDGDDDDDVEATTGGLLYDEEGIARYLGETSGATFLNVLKQFMRSLVLVTYELDVMNGSLFTETIGSYQTFDSRPLPNPTVDPLWLPAQEDMASMLLELRFYIQDGNSIFPSGGISYWGDMTRLPVVMVDWGAPLSLMADDDANRHLAFYHVCFALASSVGHRSSRRFEQHAGEAYFMRARQLLGNPLSSVKFTLGDVPALALMGFYLIELNRRDAAYVDVCLAIHIAMMHGAFRYATDEGTKRILWNLYIMDRWLSVLMGRPPTLLDEAITLPPPSDVASMPPAAGLRARIELARISGFIVCETYKIAPRLCSPGYASSRHIDKVLKRLDDWKSQLPPTLTIPHNPLNPDPACCLLHMERNQLIVLTIRPMFLAAVKQAVGNHFISGHWSVEQHPHRSHLQACSAAAHENLVLAQQTRPSRKLLQAGLHFVFNAAIILLLDRLVHGVTGYGTGHSNGHLMSTPPGEHLHALGIDFAIDVFEQESKIGTNYPRDCCRVLRDLRALVGHQLSQTQLDIQQDDTTIPTRTRNGLPMQSGNGQSPGAPASQPTFSEESELYQEIITWLQEDGLQLRDDLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y364</accession>
    <name>A0A9W8Y364_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y364"/>
    <dbReference type="OrthoDB" id="20729at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21099">
        <property type="entry name" value="RAD201"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21099:SF2">
        <property type="entry name" value="SI:CH211-113E8.11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="331"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="397"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="505" mass="51843" checksum="887EDF5A02826581" modified="2023-11-08" version="1">MNNDLTGQRPIYPLSCYGPGRDAPRQLIEGPVEISPEELRLRYYTLRAAGNEPTAQQEESALNEKMQQQVKAILDDIDGAIRYVEEGANVHPNRIDIAKGNAATNAPANALSAPSSAGSNSNPFLKAPSNALSTGASAQTSAFGQASTPGFGRPAFGQPSNPAQTTSAFGQPSNPGQAASPFGATSSLGPKPSPFGQPSALGAAGGFAKPAFGASGFGQTSTPGAGGAFGQPSSLGQGSAFGQPSAPGTTSAFGQANPLGQKPNPFSKPGFGQSAFGQASQPGSNASPFGQQAQSNASPFSQQAQSTTSPFGQQAQSTTSPFGQQAQSTASPFGQQPQQGTQTSAFGQQTQPNQQANPFGQAQSPKPASPFGGATSTQTSAFGQSSLAAPNVSPFGTQAQNTSAQQTSNPFLQKTQGAAPSTTTAPPTQAQNPSPFAQPQTQAQQPVSTPADRFKEGKPEDYEGEKGRILEEIYKGVNQLGRFNDDEDIPLVPPKCEWIKPFTFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y399</accession>
    <name>A0A9W8Y399_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">tRNA A64-2'-O-ribosylphosphate transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RIT1</name>
        <name evidence="4" type="ORF">N0V83_007394</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y399"/>
    <dbReference type="OrthoDB" id="45256at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043399">
        <property type="term" value="F:tRNA adenosine(64)-2'-O-ribosylphosphate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019988">
        <property type="term" value="P:charged-tRNA amino acid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007306">
        <property type="entry name" value="Rit1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033421">
        <property type="entry name" value="Rit1_DUSP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033449">
        <property type="entry name" value="Rit1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31811">
        <property type="entry name" value="TRNA A64-2'-O-RIBOSYLPHOSPHATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31811:SF0">
        <property type="entry name" value="TRNA A64-2'-O-RIBOSYLPHOSPHATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04179">
        <property type="entry name" value="Init_tRNA_PT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17184">
        <property type="entry name" value="Rit1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rit1 N-terminal" evidence="3">
        <location>
            <begin position="29"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Rit1 DUSP-like" evidence="2">
        <location>
            <begin position="338"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="460"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="486"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4366866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="661" mass="73091" checksum="7B76420317012E1E" modified="2023-11-08" version="1">MMSQPLSEADIIFPHLSNTPNLSSTLSSLRRSTLSIHNRLSSIVFDSAFVTSVAEAYAFPLVANERCGSWYIPLSLKSESVYFKSTDGHMGEWKFSLRRLNLQLLDVVEKWGGAVIVDSTRRGKSVPDALSKTVPIWCCVINRAVFGEKHDLFTWPQAVGESEHAQIERRIGGFVRQFLDICKPNIPHLRAKLQKPLRPIWVTQQSSLPNSPPLFSDFHPIVLCTASRRVRGAEGSENGYIQGAADDHEAWAQGLTPTLFWQNKNILTNASEEEAPGIIERLIAEKDGDHATATLIKPTNRLYISSSHSVDPRGFDLVISCTAESFPQSLLKDANVQHYLHLKCQTGKLGSRDLRSRLPHLLAIFTSFPLSEHSRVLFCCPTGKDLSVGTLLAILCLYVDEDGTLNIGKRRESKDIGKVFIKQRLSWITTSNPALNPSRATLQSVNAVLLESENPKAHLQTNKLSNDKPTFPTSIAVRDTKPISHEPTSTNNDRSPSLPTSIFHNLATPSSSDRRWLFTRTLRSSLPTHPSGSVIGTATFTPCSFPSPSPSLSDTATSPSTMQQALLYAEEGKFVTHTGLQFTARRKYMYLLKRDVNSAAEAEAKPKRHIAVHFFDDDKMPGSSILEGVGKDSAGVGGLFVEMDTLEESREDVLFLSGNDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3N6</accession>
    <name>A0A9W8Y3N6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007943</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3N6"/>
    <dbReference type="OrthoDB" id="10265837at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1050.10:FF:000001">
        <property type="entry name" value="Putative Non-specific lipid-transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="entry name" value="SCP2 sterol-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094:SF25">
        <property type="entry name" value="SCP2 STEROL-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094">
        <property type="entry name" value="STEROL CARRIER PROTEIN 2 SCP-2 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP2" evidence="1">
        <location>
            <begin position="20"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="129" mass="13559" checksum="BC90B5379EF26B45" modified="2023-11-08" version="1">MSLKSADFPSSAAFDEIARSLSSSDADRTDAIKKGGAIFAFTLKNTAGAQESWYIDLKESGTVGKGLAPEGKKQAVTLILSDKDFGSLVAGKANAQKLFMSGKLKVKGDVMKATKMEPILKKAQSGAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3R3</accession>
    <name>A0A9W8Y3R3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_008362</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3R3"/>
    <dbReference type="OrthoDB" id="44467at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071913">
        <property type="term" value="F:citrate secondary active transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006843">
        <property type="term" value="P:mitochondrial citrate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000092">
        <property type="entry name" value="Mitochondrial tricarboxylate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000064">
        <property type="entry name" value="Mitochondrial tricarboxylate transporter (Ctp)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049563">
        <property type="entry name" value="TXTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788">
        <property type="entry name" value="SUCCINATE/FUMARATE MITOCHONDRIAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788:SF4">
        <property type="entry name" value="TRICARBOXYLATE TRANSPORT PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="11"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="98"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="198"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4365742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="295" mass="31941" checksum="466054252388C45E" modified="2023-11-08" version="1">MAAVTTEKKKPSAMRSILAGASAGAVEISITYPAEFAKTRSQLNRRLPDGKKLPWPPFGAQWYAGCTTLIIGNSLKAGIRFVAFDIYKDLLSDAEGKISGPATVIAGFGAGATESLLAVTPFESIKTQLIDDRKAANPRMRGFLHGSRIIAQEKGIRGFFQGFLPTTARQAANSAVRFSSYTSLKQLAQSYVAPGEKLGAISTFGLGGVAGIITVYTTMPIDTVKTRMQSIEARSLYKNSFDCVAKIFKHEGLLTFWSGAVPRLGRLILSGGIVFTMYEKSMEFMDKLDPQGRYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3Y2</accession>
    <name>A0A9W8Y3Y2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008093</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3Y2"/>
    <dbReference type="OrthoDB" id="3231000at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="257"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="321"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="356"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="161"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="387" mass="44153" checksum="F8B7BBEE41B388D1" modified="2023-11-08" version="1">MSIIIVNDVWSTQTFRTNKSRDGHITFHNPRSISSMLSGDSWTSLSDGTIDTFAKLLVLDFFSASIYLQPVDYYGPLIYPKLPEKEVVKFSLTKNAHLQVPLGLFEPRLSAKSREAFKEIEDAEGILRSVDNLVDSILFVVAALKQHSIANSQQQQQLVRKDDHERRMQKLEGLCSERRNNARRALEALNRQLDYLSKRHAIREAKAIKILTVLASLYLPLSLSASLLGMQTPFRQVAHTQTPAEQDLVGTNLLFDFFGVFVGLATATIFIVHAIRLILWLRSNGLGILWRTFSGPFSIFHYGRRWRYGGREGRVFDLIRVLTAWWIGAGFFITLLVIFMVGMLRTAQNAWDTAKGMFTAYCVVGGILMGLYVGVYWYLYYKRIRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y464</accession>
    <name>A0A9W8Y464_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">Multifunctional fusion protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="10">Delta-1-pyrroline-5-carboxylate dehydrogenase</fullName>
                <shortName evidence="10">P5C dehydrogenase</shortName>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">L-glutamate gamma-semialdehyde dehydrogenase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="9">L-glutamate gamma-semialdehyde dehydrogenase</fullName>
                <ecNumber evidence="9">1.2.1.88</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="12" type="primary">PUT2</name>
        <name evidence="12" type="ORF">N0V83_006950</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 9">
            <text>L-glutamate 5-semialdehyde + NAD(+) + H2O = L-glutamate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:30235"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="EC" id="1.2.1.88"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.88" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y464"/>
    <dbReference type="OrthoDB" id="5322683at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003842">
        <property type="term" value="F:L-glutamate gamma-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07123">
        <property type="entry name" value="ALDH_F4-17_P5CDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000006">
        <property type="entry name" value="1-pyrroline-5-carboxylate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000005">
        <property type="entry name" value="1-pyrroline-5-carboxylate dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005931">
        <property type="entry name" value="P5CDH/ALDH4A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050485">
        <property type="entry name" value="Proline_metab_enzyme"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01236">
        <property type="entry name" value="D1pyr5carbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42862">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42862:SF1">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0520">NAD</keyword>
    <keyword evidence="3 8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 9" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="11">
        <location>
            <begin position="94"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4367369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="584" mass="63544" checksum="444AD0C32CE6252A" modified="2023-11-08" version="1">MGSSKGLQPLRSVSKSFNALPAAYAGAQIASRRYAGTIANFKVPTIDNEPNHHYTKGSVDRQKLQDAVAALQKKGPVQVPLAVGGEYINNSSILTQHNPSSHADVVAKYSNASRADVKKAIDAALEAKPAWESLPFAERAAVFLKAADLVATKYRYEIMAATMVGQGKNAWQAEIDAAAELCDFLRFNVKYAEETYGHQPVHNSPGVWNRVEYRPLEGFVYAVSPFNFTAIGGNLPAAPALMGNVVVWKPSPSAIASNWLIYQILIEAGLPPNVIQWIPGDAVEVTQEVLSHRQFAALHYTGSTAVFRQLYGKIAGGVAEGKFQGYPRIVGETGGKNFHLVHKDADISNAAIQTVRGAFEYQGQKCSACSRLYVPESAWPEFKQKLVTEVEALKIGEPTDFSNFIGPVIHEASFKKLSGVIDEAKNDSELELVVGGKYDSSKGYYIHPTIYRTTNPNHPLLSRELFGPILVTYVYDDSAPDAFANIIKTIDDTSEYALTGAVFAKDRETIKFASDKLRDAAGNFYINCKCTGAVVGQQPFGGSRASGTNDKAGSANLLARFVNMRAVKEEFLPTEKVTYPSNEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y476</accession>
    <name>A0A9W8Y476_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2 type master regulator of conidiophore development brlA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">CRZ1</name>
        <name evidence="15" type="ORF">N0V83_008805</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y476"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000146">
        <property type="entry name" value="C2H2 type zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000181">
        <property type="entry name" value="C2H2 type zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000239">
        <property type="entry name" value="C2H2 type zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF19">
        <property type="entry name" value="YY2 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="496"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="524"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="170"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="343"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="669"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="43"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="185"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="422"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="437"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4365187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="726" mass="79026" checksum="F34B9BD52E713476" modified="2023-11-08" version="1">MDIQQRGRSPSAGQRNNIRHSPSPSPHAPFHQNVSGLGFDPSLSVNDPSTSFQVTSAGFDTSQYLTTTQGQQYQQQPALADQSFLQSTQNLSQHNTPHFGPQGTNISPDPLDIANSNNPDFNNFFAANDINPSQGINPSYLGNTLDPQLLDAQSQHNQSISASDLMNQMATAQQNAPTPPHLLPTMNNRQTPSPQASPNMNQGQFTSPGHSRNASLDPNAAYGQGTEWGNMANFRGHRRAPSETYSDVSSAHASPYLVQQDSFGETDQPSPLLNAQNDPQLFHDPVMQFGQFNLNDSNAHISPGHSPHISPRLIPQPQQALPQFQPGSFGLDQSMNNQFAQQGMGTYQNQGPEPFPSLNQPVPEFGQADAMSPPEINIDFAPPSRQASFEPPKPEHQADALSPPDRSRSRNRIRAKSDPFSGASSRASTPGSEVHRSLSPSAAKSRSPSPSSKSSRRSSTSSVPNRDYILDLADPSRPAPADGSNPKRTQKHPATFQCTLCPKRFTRAYNLRSHLRTHTDERPFVCSVCGKAFARQHDRKRHEGLHSGEKKFVCRGNLKDGNHWGCGRRFARADALGRHFRSEAGRVCIRPLLEEEAQEKGGQWDPQNQQMNNGNGMFAPMPAQDFGGGMMPQPGFEPFPQAGGMQPPPQFLPAALLAQYPALAGIQWDQIPAGGPDEVEGDISGRSSFDASSGGEFFDEESDYQNQQPIYGGNGGGWASDSYDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y548</accession>
    <name>A0A9W8Y548_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y548"/>
    <dbReference type="OrthoDB" id="7464126at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="256"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="341"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="788" mass="87937" checksum="C6A3ED51824E139C" modified="2023-11-08" version="1">MSFGVSIGDILRLCELAGRVYKNCRDCTGEYKVLTTESRTLSNLLEDIQDKYDKIPEHKRQQLVDAYEPCIDVLQELDKLLLHYNSLDTKSKRAWDRLKWDPDRSRILRERLTSSISMLNTFYTSLIHDSQVLILEALERLEKDYKGGHREESIASIEQIASHGDEADDDAAWTQILRDLQDVGISQQEALSYRNIIVDWLVTAVNDGRLLEQRPQHHGLASLSHDLGTALPPLDRMEERESYTPEPSGQRNTSAPLPTLPLLVSPPLLEDQRDQGNPSAGSLPISGQSNTSIHTTSPSSDVDTSSERVSVPQPTASNVQPGSTTLPEAPPSPLEAPSTPPTTSSFPAHTASDLPPTYYEKGTSITLDLDWTAQQIVAAWSRRDFHTAERLLGEQLAAVERGQTVSSGTQPDRRILRHLIGICASFTGNFTKAKRFFDSVFNGIYLNRASLDDGDIAAARWLGDVCLHLREHNNAVLAYSVAFEGSIGRFGIARDRTRRLSEELRLLDHWLYAFRRVEQSFSLNTDPTDIFASAHAVEKSNLTNSVKAQLYSTSGSGDGYPAPPLYQFLRPSFLITTRPRTELMLSENFLLAPLISLSAWPFPWDPTFSPADAVQLNRYMNTVPSAKGVVPLIERSLSTTKLGESKKLHYVTKRSSQWLIQSVKTGLQDMGIEHAEHGYETSIVCCLNQHRDGFAFSEGIQICFSKLQFRNVYGLKVKDVHWATRRIAATSIKAAQLQKDTTDFRDIVKGILERAEADVGNPAPESTTHNVYPVLLQTPGSEKRPIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y574</accession>
    <name>A0A9W8Y574_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_008457</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y574"/>
    <dbReference type="OrthoDB" id="19923at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000034">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000068">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF52">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G11820)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="315"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="339"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="401"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="433"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="43"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4365836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="489" mass="54306" checksum="49D8E7ED6D7975F9" modified="2023-11-08" version="1">MAHEDVKASLPVDEKIETINDTHGYHIDPVAEKKLLRKCDLRVLPPLFILFLLAFLDRTNIGNAKIQGLVEDLDMVGKNASRYNIALFIFFIPYILFEVPSNLILKKLAPSTWLSLIMVLWGIATMGQGLVNTFGGLVAMRVLVGIFEAGLFPGCVYLISMYYKRYELQWRLTLFFTASIIAGAFGGLLAFAIAKMDGVAGYGGWRWIFIIEGLVTVFFGCLTKFWVADWPETAKFLNEDERALLIARLSHDTGDAVMNRLDKRAARRIFSDPKIYLGTAMYFGIVNTGYAGSFFVPTIVKELGYTSSAAQVRSIPIFVVATVTAIITAYLTDRMRHRYWFCISGLVVASIGYIMLLAQNHLSAGVKYFALFLIVPGGYITQPVVLVWMSNLVSGHYKRSVSSAMQVGFGNLGGIVASNVFFASEAPKYPTGYGVSLGMLWICGAACTAMFFLVKVENGKRERGERDARLQELDADNLGDDHPHFRLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y577</accession>
    <name>A0A9W8Y577_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Urea carboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_007732</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y577"/>
    <dbReference type="OrthoDB" id="196847at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050856">
        <property type="entry name" value="Biotin_carboxylase_complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003778">
        <property type="entry name" value="CT_A_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003833">
        <property type="entry name" value="CT_C_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00724">
        <property type="entry name" value="urea_amlyse_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866">
        <property type="entry name" value="CARBOXYLASE:PYRUVATE/ACETYL-COA/PROPIONYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866:SF128">
        <property type="entry name" value="UREA AMIDOLYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02626">
        <property type="entry name" value="CT_A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02682">
        <property type="entry name" value="CT_C_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00796">
        <property type="entry name" value="AHS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00797">
        <property type="entry name" value="AHS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160467">
        <property type="entry name" value="PH0987 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0092">Biotin</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="9">
        <location>
            <begin position="1"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="8">
        <location>
            <begin position="107"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1085"/>
            <end position="1119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4367202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1187" mass="130828" checksum="B23B8C6E9F8082FC" modified="2023-11-08" version="1">MHVRLCRTAKKLNIRTVAIYSEADAASQHVRDADEAVLLPGSNATAYTDEEAILQIAKEKGANAIIPGYGFLSENARFARLVTDAGIAWVGPSPESIEAFGIKHTARELAEEASVPIVPGTKGLVEDEEAAAKEAERIGFPVMLKATGGGGGMGLITCNVVEEVRDGFRMVQSRGKTLFKNPGVFIEAFYPASHHIEVQVFGNGQGQAIHFGERECSIQRRHQKVIEECPSPFVEKHPELRKKLGDAAVRLAESIRYGSAGTIEYLVDDSSGDFFFLEMNTRLQVEHGITELCYGIDLVELMLEQADAQLAGKGGLDGDKLKRMQPGTPCGSAIEARIYAENPLKDYAPSPGLLQKVEWKDVPGSRVDTWVFTGSRITPNYDPLIAKAMVHSQTRAETIDSMRNLLKGSSICGPPTNLEFLASILDDSRFKAGTTMTSFLKDLKYAPQAIDVLSAGAYTLIQDLPGRPTVGKGIPHSGPMDPMAFQIANILVGNPRTTEGLEITLGGPELRFVGPAIVALCGAPMEATLDGERFPMWTSVKIDAGQKLKIGKTTGGGCRSYLAVHGGFPAIAEYFGSKSTSPLVAIGGYQGRALAPGDLLQIAKQLPSDFKAVSLPEHLRLTYKADWEIAAMVGPHDEGYFDPDFIQTIYDTEWKVSHNASRSGIRLVGPVPKWARKDGGEGGAHPSNLIEYGYPLGTLNWTGDDPCIFPVDCPNFGGFTSSTTVIKADWWKLGQIKAGNTLRYVRVGLEDALKKRKRNDAFLDSVEQATKSNNGFKKVEKLNKVSFHEGQIGKAVLWEREGDGHVPQVRYRQGGDDHLLVEYGNESFDLNHRCRVTALENALNSNNSPDVIKSNLYNTVGCCTTLLLYYNGVRLPRSDLVAHLQKIESQLGDLRTTKVPTRIFKLPISFESKLQDEATQRYMTNQRPHAPYLPDNLAFVAQNNAFTPQQLKNIYLTGQFMAVVVGFFCGNTVSLPVDPRQRLSAPKMNPSRVFTPEGTVGWAGSCMSIYPVDSPGGYQMTGRTVPCWDYYSYKPGFAQDARPWIFRDFDILTFYQVSEKELDSLLGKFRAGKYDWEYQEVEFDMAEHNDLLAKTRDEVTQIRKKQEVAQEEMTKAENESLERWRREKAENQVDEGTVENLLGDTNIVSVEAPVDANVWKVEVKEGDNVAEGTVVSVILSVNLRKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y591</accession>
    <name>A0A9W8Y591_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006655</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y591"/>
    <dbReference type="OrthoDB" id="3794585at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="265" mass="29795" checksum="486FC926E91DBA89" modified="2023-11-08" version="1">MTSLRLVSPQRLQDHVTSKKKAHSSPRKVCKPRDSHFTIVQPSRAPKSVHRSLPTLKNGKQQLPPHFKAVSDGFVGSELKTEFKKRSHRKSKAAPIQRKIASARGLALFSGLLPLPAYDRIKAPMECSTPSYSNPPPGFVVSAEQFRDITSPEQLMASTQNTDHHRQTPRPHPQQWDKAIYNQLSSVTAPVRSVSENSQSELSAEERSENGEEDSGADDDFMNSQRLVPWTPMDVHSIADEPTYFEHAIYHLNVSDHALYVDDHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y595</accession>
    <name>A0A9W8Y595_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inositol monophosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y595"/>
    <dbReference type="OrthoDB" id="10254945at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854:SF39">
        <property type="entry name" value="PROTEIN QUTG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="80" mass="9052" checksum="636DCA73DA2B90BB" modified="2023-11-08" version="1">MAEISRDELDEIYEFALELGKQAGKMLTDAAQVRISGGNGTQEEKEHVQKENAVDLVTETDENVEAFIKREITKKYPSHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5R8</accession>
    <name>A0A9W8Y5R8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_007154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5R8"/>
    <dbReference type="OrthoDB" id="3797124at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4367570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="155" mass="17777" checksum="F6532BF7FB92636B" modified="2023-11-08" version="1">MQDTIPSSDTSTRGDVLFQDLGPIKLLKDLRILHLYRHPMQRVAAVDAHQFAIRFFQTIHLHCPFLNVLILGSCDTSSDDEIHTDLDMLNDAGVAIKGTSQYRYVKREETLIDGQVQMTAVAVSLSQLRDEFPHLEMLNYDPGHQLLERVFHQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5V3</accession>
    <name>A0A9W8Y5V3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_007428</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5V3"/>
    <dbReference type="OrthoDB" id="3357846at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015244">
        <property type="term" value="F:fluconazole transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF23">
        <property type="entry name" value="FLUCONAZOLE RESISTANCE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="250"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="305"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="453"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="493"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="518"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="552"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="588"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="179"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4366899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="621" mass="69298" checksum="E3D0DCE0228A1B87" modified="2023-11-08" version="1">MVAALIRDAPIGQVIRYITKNRVLQYPEERPDFQVPAAYTRHARPVPKRLGTNATDESQLEPEGPATEEVVYPDPETILQLEELDTISDEKETELEKLQTARTQHTAKTQISRVGTRTALQKSISRADLEQQFSLASVERGPSRPIEPETLDDGTILVDWYDTDDADNPQNWSFHKKNVVLAQILMYTMGVYMGSAIYSPSIPGVMQRFGVEIGAASLGLSMYVLAYGIGPLLFSPLSEIPIIGRNPPYMISYAIFMVLLVPTALVDNFAGLVVLRFLQGFFGSPCLATGGATLQDVYSLIKLPYVLSLWAFAATCGPALGPIISGFSVAAKNWRWSLWEMLWMNGPIFLSLFFFLPETSSANILLRRAQRLRKLTGDNRLMSQSEIDQANLTARDIAVEALWRPFQLMLLDPSIAFTAVYTAIIYGIFYSFFEAFPLVYNEMYHFNLGELGLTFLSVTVGVILSITWYWWYIYYRVEPSIRANGLGSPERRLIPALFVTFFVPVGLFIFGWSSNPSVHWIVSCIGIVITTIGIFLIIQCIFLYLPLSYPQYAASLFAGNDFTRSALAAGAIHFSYPMFHNMGVDRGVSLLAGLTVGCSIGVYVLFFFGAKLRAKSRFAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y601</accession>
    <name>A0A9W8Y601_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Signal peptidase complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_007504</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Dispensable for SPC enzymatic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPCS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y601"/>
    <dbReference type="OrthoDB" id="263893at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009542">
        <property type="entry name" value="Spc1/SPCS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13202">
        <property type="entry name" value="MICROSOMAL SIGNAL PEPTIDASE 12 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13202:SF0">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06645">
        <property type="entry name" value="SPC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4366974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="102" mass="11039" checksum="D1068D68523932D8" modified="2023-11-08" version="1">MSEQLLEQVRDAVEGQIDFEGQRLAELLTTVLLGAAGIIAFLVGFMTQDIRLSLYIGLAGTALTFLVVVPPWPFFNKNPEGWLPPHKATSGISIDVDGQKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6C5</accession>
    <name>A0A9W8Y6C5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Acyl-CoA dehydrogenase NM domain-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_007684</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6C5"/>
    <dbReference type="OrthoDB" id="434771at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase fadE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF32">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE NM DOMAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="9">
        <location>
            <begin position="41"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="166"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="280"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4367154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="477" mass="53021" checksum="EDC1127AAB3867A7" modified="2023-11-08" version="1">MSVPYDRAYDLLSNSQNGHISASQRIPTIARPFVSERAAKTLDIVEKFVEEECIPADAVYTRQIGQTTQERFSSHPSIIEDLKKRAQELGLWNMFLPKAHFKEGAGFSNLEYGLMAEYLGKSRTASEAVNCAAPDTGNMEVIAKYGTEQQKRKWLDPLLKGEIRSAFLMTEPQVASSDATNIEMTIKRDGDSYVLNGQKWWSSGIGDPRCKIFIVLGKTDPNNSDKYKQQSVILVSNDTPGVTVHRMLSVFGYDDAPHGHGHVTFENVRVPASNMVLGEGRGFEIIQGRLGPGRIHHAMRSIGSAEKALEWFLARINDERKKPFGELLNKHGIMLERVARSRIEIDAARLAVLNAAIKIDETNAKGALKEIAEVKVLVPEVNLKVIDWAIQAYGGGGVSQDTPLAQMYASGRTMRIVDGPDEVHLLQLGRNENKRGVAHKKRIEAQKIKGEELCRKYGIEPKDPLYLNRTSGEASKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6N8</accession>
    <name>A0A9W8Y6N8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosomal protein L33B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RPL33B</name>
        <name evidence="4" type="ORF">N0V83_007581</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6N8"/>
    <dbReference type="OrthoDB" id="1166329at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.190:FF:000001">
        <property type="entry name" value="60S ribosomal protein L35a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.190">
        <property type="entry name" value="translation elongation factor selb, chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00573">
        <property type="entry name" value="Ribosomal_eL33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001780">
        <property type="entry name" value="Ribosomal_eL33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018266">
        <property type="entry name" value="Ribosomal_eL33_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038661">
        <property type="entry name" value="Ribosomal_eL33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10902">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01247">
        <property type="entry name" value="Ribosomal_L35Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01105">
        <property type="entry name" value="RIBOSOMAL_L35AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4367051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="104" mass="11628" checksum="427F21EE76061B36" modified="2023-11-08" version="1">MSDKSHRLYVKGKHISKRNTNPGTSLLKLEGVDDTKAAQWYAGKRVAYVYRAQRAIQGSKIRVIWGKVTRPHGNSGVVRAKFRSNLPPKSFGASVRVMLYPSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y702</accession>
    <name>A0A9W8Y702_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peroxisome assembly protein 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Peroxin-12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">PEX12</name>
        <name evidence="18" type="ORF">N0V83_006492</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the PEX2-PEX10-PEX12 retrotranslocation channel, composed of PEX2, PEX10 and PEX12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y702"/>
    <dbReference type="OrthoDB" id="107372at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016562">
        <property type="term" value="P:protein import into peroxisome matrix, receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017375">
        <property type="entry name" value="PEX12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888:SF0">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12 PEROXIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Pex N-terminal" evidence="17">
        <location>
            <begin position="25"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="309"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="419"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="318"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4368137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="468" mass="52409" checksum="E36266A90A100CD7" modified="2023-11-08" version="1">MEFLPTLQHGVDDLKPSLFELLSEQQLASLLPPSLRYLLAVSTHRYPRYLLPVLNSFDDVYAVLMLLVERHFLRTYGGSFTENFYGLKRARVLKVKGGEIPRAQLGASDSVREAVELGEGDVWKNLAVLVGLPWLKRKLDEGYDVHAAHANLLGPGYNRERDGLRAGASIKEKLMYYYKWFLRNVYPSVNAAYYFSLLVFNMAYLFDDTKYHSPFMWLIGSRMRRLGEADYKAIELATAPRKVGPARPGEGGSIFSPRNLARSIQPRLLSSLKILLPTSIFALKFLEWWHASDFARQLSRKAAENIDLPPPILPSLPHSTKPTTASKQTHSSEKLQPSSIVSNNPQRIDPPISSTTLLPILTVSRPPSSHLCPICVSPIMTPTASPTGFVYCYTCIHRWVEGEHDRQVAFMEGAAGLRNGEEGEDVEDGGWSREEGSREGRWESGKGRDAVTGRRVLGGTEGLRRVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y721</accession>
    <name>A0A9W8Y721_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acyclic terpene utilisation N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005321</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y721"/>
    <dbReference type="OrthoDB" id="10265871at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010839">
        <property type="entry name" value="AtuA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47585">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47585:SF1">
        <property type="entry name" value="DUF1446 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07287">
        <property type="entry name" value="AtuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyclic terpene utilisation N-terminal" evidence="1">
        <location>
            <begin position="7"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="547" mass="59947" checksum="A0939537BD4FA747" modified="2023-11-08" version="1">MGSKRAIRIGNASGAIGDGLDQVYRLARDGDVDAITADYLAEFNLAWKAIEMIDRPDLGYEPHFLSQLGWKDGEAARIVAQKKIKIVHDGGALNPEGLAEKVHEYLVSQSIHSLKVAWVSGDNLTKHVRSNKDASYQHLDIKGQSMEGNKDMILAANAYTGQSGIVAALKAGADIVICGRCCDASPVMGLASWWHDWDTKDYDKLAGSLMAGHIIECGAYATGGNYCGAREIPKQYRVGYPIAEIDGEGESVITKPANSNGAVTVDTVKAQFLYEIQGSRYLNTDVIARIDQANLEETGPNRIRMTGITGFPPPPTAKLAIFTRGGYQAELSGYCAGLEIDAKVNMMKTQILRELNPADFTTLDIRSYGTAATDPKTQAEATVLIRMFVQANTTEAIERFRQAIFYNGMQGYCGLHLAMDWRTIEPRLFVKYFPALIDQRIIKLSVDFVGDQSAKIASVPMLERFGRPFPGQESYETRNPVDIGSFGPTTKRPLGDLVFGRSGDKGGNANVGLWVRDADAYPWLQAFLTIPRLIVLLGDDWSSKKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7K7</accession>
    <name>A0A9W8Y7K7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006809</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7K7"/>
    <dbReference type="OrthoDB" id="3683755at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="528" mass="58854" checksum="40FC949B8FABEA47" modified="2023-11-08" version="1">MATATLSIRPSPTFNWADDDEDDFDFETWKARADTSAPTVEDLGPLQLAPTAEDNSPLFTVTATAGSPKLSTNPSFNWADDEEDDFDFETWKAAADTSAPTVEDLGPLQLAPTKEDASLFTVTATTTSPKLTTNKQCTAPCASAEQKESPASEIEMSSCAHDQPSSLEDRGDTDAPVDGWYRAEAQACLVYRGLLESSDAPAYRWMSYYDNGFPSPFKRVNYSQNWSRMKADSGLDARFPVQYKKSPLQQVMFVDEKEEEVAVVAGEDVEIVVDVGEVKEDVVETSSEEIKKIALDEEYENEDEQFSEIDLKDLPQLQHHGDSSSETDEEEDDIRKVAISIKKPDLPAVAVAKSAEGDFDFSDVFKDEDEDSANIQDEGYHSSSPPISPTRSTCDENLDPILSLTSLEMQQSSLSSIRRKRHERHDSMDALLSFRKSAEDLVAVDNALDDDNDEPHTSTTTTPHHHHYYYNLYQPPSPLNHLSPHDQQRHGAITRPQVHHPRPNLHRLRIRHSIHGLVLPHACTLDDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8C7</accession>
    <name>A0A9W8Y8C7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PSP proline-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005700</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8C7"/>
    <dbReference type="OrthoDB" id="10260794at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007180">
        <property type="entry name" value="DUF382"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006568">
        <property type="entry name" value="PSP_pro-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052584">
        <property type="entry name" value="U2_snRNP_Complex_Component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785">
        <property type="entry name" value="SPLICING FACTOR 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785:SF6">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04037">
        <property type="entry name" value="DUF382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04046">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00581">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSP proline-rich" evidence="2">
        <location>
            <begin position="275"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="567" mass="63318" checksum="0B76355B721E3B86" modified="2023-11-08" version="1">MSGATAKAKKPPAKNAWRRAQKKQKRSETPSAAGDSARESESPAPADTDSDAHAVRPADLLSVDDIANQYNLDDPVFEQYKSVFEKFREPTEEENATKDVEKPAVFYEDDDIPDEDEEDQLKVSKKQRKAMNKLSVAQLKALVQRPELVEWTDVSSSDPTLLIAIKGAKNVIPVPIHWSLKREYLSSKRGIEKPPFALPKFIQETGIAEMRDAVLEKQAEMTMRQKQRERVQGKLGKLDIDYAKLYDAFFRRQTKPELTRYGEVYYEGKEFETNLVNLKPGELSEELREALGMAPGHPPPWLINMQRCGPPTSYPNMRIPGVNAPIPPGASWGFQPGQWGKPPTDDAGRPLFGGDLYGTSILEEQQQPTHIGEPVERGTWGALRAEGESEDEESDEEEEEDEDEEEGEGDLSGVRTSMTTASAMPSEFGGTESIGGEFTLRKQRKGIETEEPGAPRSAFTVLPEKNISATGFFGGEHAYDLDAARRDTLGQSQRKRKAGDVDISVDVDQLADSDRLDKDALKKEYESRQKAEAQGQWQSIDQDDLADMIAAESRKRVKRETEGKSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8H7</accession>
    <name>A0A9W8Y8H7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_005895</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8H7"/>
    <dbReference type="OrthoDB" id="5296at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05325">
        <property type="entry name" value="carb_red_sniffer_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052184">
        <property type="entry name" value="SDR_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45458:SF1">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45458">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE SDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4368813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="241" mass="25870" checksum="4FD4F2B03567054B" modified="2023-11-08" version="1">MPTAVITGANSGIGNALAQILVKESQALRHMQGYKVIAADIELGDPIKSLGCENIKLDITSPDDIQNFKEQVGDQPIDMLLNIAGVMSPHSSDTLQTVSLPTFQATFAVNTFGPLLLTQALLPNILLSPHPRIAVMSSRVGSMGDNSSGGSYSYRASKAAVNSLFKSMAVDLREKNVPVILLHPGIVKTNLDKRWGEGGALDFKGAVEPEVAAGELWRVLMGKGMESTGKFYHRNGEELPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8V3</accession>
    <name>A0A9W8Y8V3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA-directed RNA polymerase II subunit RPB3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rpb3</name>
        <name evidence="10" type="ORF">N0V83_006075</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8V3"/>
    <dbReference type="OrthoDB" id="270173at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07031">
        <property type="entry name" value="RNAP_II_RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.120.12:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001988">
        <property type="entry name" value="PRK00783.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF2">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="9">
        <location>
            <begin position="21"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="321"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4368993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="335" mass="35924" checksum="0E8E4D63E15DECB9" modified="2023-11-08" version="1">MDYDPEGGAINVRFLQADNQRANFIVQNFNLETANSLRRVMLAEIPTLAIDVVEVLDNTSVLADEFVCHRLGLIPLSSRGVEDLLYSRDCDCEGYCDNCSVVLTLNAKCTGSEIMKVYARDLVIESNRPNDEVGRPVITDEAGTGSCIVKLRRGQAIHMRCIAKKGIAKEHAKWAPSAAIGFEYDPANKLHHLDYWYEEDPEKEWPKDEERINLDGGPANPDEPFNYDAVPTRFFYDVETVGGLDPDQIVTKGINVLQQKLAGIIHELSGGAGGPDGVDFGGAQSPTLNGGGYGGADGGYTPAGGYGGASSWGGAAGAAQGGTTPYGATPYGQSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9G7</accession>
    <name>A0A9W8Y9G7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">nicotinamidase</fullName>
            <ecNumber evidence="6">3.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Nicotinamide deamidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_006153</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; nicotinate biosynthesis; nicotinate from nicotinamide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.19" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9G7"/>
    <dbReference type="OrthoDB" id="167809at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008936">
        <property type="term" value="F:nicotinamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019363">
        <property type="term" value="P:pyridine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052347">
        <property type="entry name" value="Isochorismatase_Nicotinamidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11080:SF2">
        <property type="entry name" value="LD05707P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11080">
        <property type="entry name" value="PYRAZINAMIDASE/NICOTINAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040730162" description="nicotinamidase" evidence="8">
        <location>
            <begin position="18"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Isochorismatase-like" evidence="9">
        <location>
            <begin position="168"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4369070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="256" mass="28156" checksum="4FFD0FB650174886" modified="2023-11-08" version="1">MLASLLTISAALLSVHAVPFSSKLHKHTALVLIDVQNDFINGSLANPRAPAILPKLYELLDDHEWPYIAASQDWHPEGHVSFASAHPGAKKGDTVNITFLDIATKTETQSLTADHCVPGTWGAEIEEGVKARLQFLEGFRTPVNYIKKAQNHSVDSYSAFADNQYHQFTTLDSELGLHSIETIVVTGLITNACVRGTCIDGIKLGYEVVLIEDATETTSSERKAASIEELEGWVMNLTDWESANPTGLRRRSMREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9S7</accession>
    <name>A0A9W8Y9S7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Carrier domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_003726</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4371953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901336">
        <property type="term" value="P:lactone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05195">
        <property type="entry name" value="enoyl_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000209">
        <property type="entry name" value="Polyketide synthase Pks12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056501">
        <property type="entry name" value="NAD-bd_HRPKS_sdrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032821">
        <property type="entry name" value="PKS_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020807">
        <property type="entry name" value="PKS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049551">
        <property type="entry name" value="PKS_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049552">
        <property type="entry name" value="PKS_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013968">
        <property type="entry name" value="PKS_KR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF29">
        <property type="entry name" value="ASPERFURANONE POLYKETIDE SYNTHASE AFOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23297">
        <property type="entry name" value="ACP_SdgA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16197">
        <property type="entry name" value="KAsynt_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08659">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23114">
        <property type="entry name" value="NAD-bd_HRPKS_sdrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21089">
        <property type="entry name" value="PKS_DH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14765">
        <property type="entry name" value="PS-DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00826">
        <property type="entry name" value="PKS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="10">
        <location>
            <begin position="9"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="11">
        <location>
            <begin position="936"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="9">
        <location>
            <begin position="2259"/>
            <end position="2336"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="8">
        <location>
            <begin position="936"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="8">
        <location>
            <begin position="1098"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="8">
        <location>
            <position position="968"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="8">
        <location>
            <position position="1163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4371953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2346" mass="254198" checksum="1D6638EFB9F4B25C" modified="2023-11-08" version="1">MGAAKLTDSHSVAVTGLSCRFPGEGDTLENFWKSICAGESAWSEIPKERFNIDAFWSQTKNRNISKPRGGHFLKQDISKFDANFFTLPKQDVEATDPQQRIMMEVAYEALERAGLPLEKIAGTKTGVFMGSFTSDYREGLTRDPDNMPGNTATASPTTALSARMSWLWDLRGPCFTLDTACSSSLVALHLACQSLRTGESDIAIVGGTSLLLNPEWFMSLSNQGFISPDGKCKSFDESGNGYGRGEGFGCVVLKRVDDAVLAGDPIRAVIRGSGSNQDGHTKGFTVPSAEAQATLIQDVYRGAGLDFKDTRYVEAHGTGTQAGDFQETTALARTIATTNTSQNKLLIGSVKSNIGHLEAAAGIAGVIKSVLILETGLIPPSINFKKGNPKIKFEEWKLQVTTQLTPFPSKGVRRISINSFGYGGTNAHAILDDATSYLQGRGISPIPHVVSNDAPKPRLFVLSSQDREGLKRVKEPLAKYAKDKSASLGKKQTDLSLSQLAFTLGDRRSRLQWKTYAVASSLDQFAERVIDDENPALVAQSSRQPRIGFVFTGQGAQWPKMGMELMAYPVFRDRVAEADRYLQEVCGCSWSATEELQKGKPSSRLHLAEFSQALCTVLQVALVDLLQTWGIRPTAVAGHSSGEIGAAYAMGALTREDAWRVAYYRGILSSEMKKNNPDLDGSMMAAGLSQEKAEEWIAKVTKGELVVACINSPTSVTISGDTSGIDELLAMLQEAGVFARKLLVDQAYHSHHMQRVAQDYYELLSDLVPLETDGSCTMHSSVIGSIIESDELGAVNWVRNLISPVLFSTAVYDMLRPVIDGKRATENAVDLLVEIGPHAALQGPATQSLKARNIANIPYQSVVSRNQNAVETALSLAGALWAQGYKVNIQEVNGDGDKQFDAPLVDLPTYPWKHSQRYYHDDRVEQEYLHRAHPKRSLIGAPAPTMGEREHLWRGYIRLAEEPWIADHKIQGAVLYPAAGYLAMALEAASQMADPSKQISTYRMRDIQLTAAAILSKETDVECVVQLRPHNASTRDSSSTWTEFTVTSSPDGKSLVKNCSGLLLVEYEPASGSEASQERTLELEALKSQYVQAKASCNKKIDTTEFYSDLSTIGLQYGPAFANVREARNRDGQSYGLVEIPDVPAWMPQGCERPHVIHPGTLDAIFHLAFAAVMGNNTLTAMVPKSIDEVIISAQVPWSAGVQLPGYATSAKHGFRELKSDILMLDDKEHLPTVDVRGFLCAEVAGGSASNTQTAAKSITSKLVWRPAVDLLTAEELERVLAPHSEGGKVAEYVRLLHHSNPAISVLEISPSAGLLAHGDVSNVAKTREVTVTCKEYGLDFTEALSSEALNDRSYDVVVLSDVGSFDSHAEHTLSNIAQALKPGGKVCFLVDKQAVAKVQAASYAKEIRTTVFPSADHSFVIGQKSLHSNGANGTNGHAEPNRITLIQPAKSTDQARTVASDINKFLQGQGYDVETFSWGSDVSSLEGKTCISILELQDSILQDLAEGDFESIRKLILNSASVFWVTAFDDPSSKMIDGLARVVRNETPGLSLRSLHAGRASLSSSARLAKLISTAFESTTEDDEYSVKDDVLHTSRIVEDVVLNEQINDLLPGAVPTVTNMALKDAKYGLKMCIQTPGMLDSICMEVDELAGTELETDSVEIQVKASAVNFRDVMAAMGQLPDSKLGLDAAGVVHRVGSAVTKFKVGDRVAVYGHGAHRTIHRTREELCQLIPEGMTFEQAATVPAIHGTAWNALVRLARVQKGQSILIHAAAGGVGQAAIQIAKHFELEIFATVSSETKRSLLRDTYGVPDDHIFNSRGLSFVKGIKRMTGGRGVDVVLNSLSGETLRQTWHCIAPFGYFIEIGLRDILANTGLDMAPFKQDATFTFFNLNHLEEERPDILATIVEGAFDFFRRGISKPVEPLVSYPVSEVETAFRLMQAGRYLGKLVFTWGDDDVVPVVQPQKSGLKLDPLGVYVLVGGLGGLGRSLSMKLVQLGARKLCFFSRSGAKSAAAKELVSDLEQLDVQVQALVCDVADDNAVAASIAKCSQELGIIRGVFQCAMVLRDTLFSNMTHQQWLEATRPKVQGSWNLHKRLPHVDFFISLSSFAAVYGNRGQANYSAAGAYEDALAFHRRAQGQRATTVDLGIMRDIGVLAETGITESLKAWEKPYGIREPEFHALMERAIEGDINGTNGAQVMTGLATGGSAIAAGIDVPFYLDSKRFSIMARTGTQNLNASAADASAPTHTIIAAAESLEEAATAVISALVKQVAKMLQMPVVEIDVERFLHSYGIDSLVAIELMNWALKEFKSSVSVFDVLAGVPISTFASKLAAKSTVLPKALVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAG7</accession>
    <name>A0A9W8YAG7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Translation machinery-associated protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TMA22</name>
        <name evidence="8" type="ORF">N0V83_003974</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3 6">Interacts with the 40S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The SUI1 domain may be involved in RNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the DENR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAG7"/>
    <dbReference type="OrthoDB" id="277199at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11607">
        <property type="entry name" value="DENR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000014">
        <property type="entry name" value="Translation machinery-associated protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050318">
        <property type="entry name" value="DENR/SUI1_TIF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046447">
        <property type="entry name" value="DENR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005873">
        <property type="entry name" value="DENR_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048517">
        <property type="entry name" value="DENR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01159">
        <property type="entry name" value="DRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789:SF0">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21023">
        <property type="entry name" value="DENR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="SUI1" evidence="7">
        <location>
            <begin position="95"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4372200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="188" mass="20643" checksum="D6D931CE9797CED9" modified="2023-11-08" version="1">MSAETSPKQVIYCGVCSLPPEYCEFGGTTKKCEEWLEEAHPDLHSKLYSAEVLQQNMSALSVDAQKRAEKDAQKKAAKAATAEARAAETRASSKILIKRIERNKRKYVTAVQGLEAFGLDIKKVAKDFGKKFATGSSVTKIPGGGEEITVQGDLSEDILEFLVDKYDEVPEDNVELIEDKKKKKAEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAZ1</accession>
    <name>A0A9W8YAZ1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_003344</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAZ1"/>
    <dbReference type="OrthoDB" id="441444at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005290">
        <property type="entry name" value="Ribosomal_uS15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23321">
        <property type="entry name" value="RIBOSOMAL PROTEIN S15, BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23321:SF26">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US15M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4373053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="37906" checksum="E3EA2008330F82AF" modified="2023-11-08" version="1">MPPRIPVLSCLRASASSSAPSQFTLPIRTFTSTSLLRGKTVEKAQKKRISHDPYLVAQRNRKKAANLSRRAELEEGRVASLGNPVRGVPTPFVQSFESGKPITYEEGKKIPKDTLHLNYAFDPNNVERQLKRSEKLAVPLSEIGKQTTAGPYQWSDSLQYGRPKEAQKSVEDIEEQNKKDHARAAEAIARITALENGSSKDRMRVNITRCIETFGRHKTDKVFPPKAPVMQKVQGQEVHPVRPETNIAETSKRIGPDTGSSEVQIAILTAKIKTVADFLASRGKGDKHNKRNLRLLVHRRQKLLQYLRRKERGGPRWQHCIETLGLTEGTWRGEISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAZ6</accession>
    <name>A0A9W8YAZ6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAZ6"/>
    <dbReference type="OrthoDB" id="3945036at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="212" mass="23536" checksum="2C54911224FDFC5B" modified="2023-11-08" version="1">MADDILANLPATDELPSYDSLLRNLNNEELPGVEEKVEDSIEDLMRPVWKDPAQQLRNDTAADLSDGNENFLNRSTGIDRTPDGLIQRRPWKGIAHQDVPVLAPAIKRTRITYRTLKSTAEVWLFNRAINYAVFIALILGSAATAGLAVWKATIESSSNPSEPAIVYGDDFYGSVSQGLGSVLGVFCMVIPLIERNRQLQPEIPVRCPRTFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBH1</accession>
    <name>A0A9W8YBH1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="6">PPIase</shortName>
            <ecNumber evidence="6">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CYP3</name>
        <name evidence="8" type="ORF">N0V83_004228</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 6">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cyclophilin-type PPIase family. PPIase H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBH1"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000035">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="7">
        <location>
            <begin position="12"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4372454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="181" mass="19832" checksum="89D1EE2C8C3201EC" modified="2023-11-08" version="1">MAETTEDNPIVFFDVTLGGEKLGRIKMELFKNVVPKTAENFRQFCTGETKNSRGQPQGYKGCKFHRVIKGFMIQGGDFINGNGTGSRTIFGTEKFADENFTLKHDKPGLLCMANSGPHTNGCQFFVITAKNGTPHLNGKHVVFGNVVEGMDVVTKIENTRTVANPEGKPVQDIVISQCGEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBH5</accession>
    <name>A0A9W8YBH5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003213</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBH5"/>
    <dbReference type="OrthoDB" id="270970at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08681">
        <property type="entry name" value="C2_fungal_Inn1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037791">
        <property type="entry name" value="C2_fungal_Inn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052981">
        <property type="entry name" value="Ingression_C2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47052">
        <property type="entry name" value="CONSERVED SERINE PROLINE-RICH PROTEIN (AFU_ORTHOLOGUE AFUA_2G01790)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47052:SF3">
        <property type="entry name" value="INGRESSION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="14"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="599"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="752"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="948"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="410"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="623"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="723"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="783"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="812"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="829"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="860"/>
            <end position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="970" mass="107522" checksum="E6870D61456C9474" modified="2023-11-08" version="1">MATKAVNLAAFGGAHTAGIFSDMTVDGPVMGTLVVIMDRARNLPNKRTMGKQDPYCAARLGKEAKKTKTDKRGGQTPKWDQELRFTVHDSPDYHRLKVSVFNDDKKTELIGETWANLEAVITPGGGQSDDWHGLTCRGKYAGEIRIELTFYDTRPKAEKPAPEQRRESARPDGQGAAVGGPRESTPVKRRPLPSDPTGASPSPVNTPEHRGLRGVRAGPRELGSPRHHQHAEQPPQPKAQSETPSRRPVPDVSPLSSTPVSNHNTPQRPQLSQGSYSTPPNPRSSNTRINLAREESFDMSYAAPKPYEMQPLDPIPQQVRDQGHRTMSQDEMRPPRGLIQQQPAEPIHSHSAPMVPTQHSYAQDAQQERYDYQDNGYTNARSPSYPHDPYQDPAYQVAPLRTSRSRDYSQEQVSTSDTYYQNRYIEAPPQHDLGSARRRSAMQPMVEDEDDLPPPPPVHRRNASTLPHPHQQESPSYVNEAPAPLNFARYREEPSQIGYDGQPQSYSPNDYNRPPPTERRMTHPRGAEHSSSRPVSRDTMVPSPLRQETSALPSSLVPGLNPSRQEREPVGYQAPPAYETPPRLRHYSEPAEYRAITEYETPVQSHELVHHQSFPSHGSPYDYPHDPPEQPRRTSPVYDPVPVVKPRATSPGVSPRHSPNPASDRTSRGPARSMPTRKSVSPRPPPSADDFGERRLSGVAFNPDSFDVYNPRVSKSPAPGTSDDERPGSRMEYNDKGQIVTFSGRVVDASDHLPIDNWAPEPTPKGTVKEKPLRSRAQLNGSRDLEAARQREERYQRERAERERIRQAASATFDSPSNALVTTRHDFSAHNSPANSGSLVLADYDATPPGSVGRNRLQKRTQQQRPMSSYDSPPQHNSPSLPMSDSHVLRERQVPNSYGGSPGYDRSGGAAGGGYGGARAPPIPAKIPLEAGRPVQNEDMALSLELQSIDIGPGSGGRRPRAQARRQYGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBT1</accession>
    <name>A0A9W8YBT1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_003892</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBT1"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000843">
        <property type="entry name" value="Nucleic acid-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051106">
        <property type="entry name" value="RNA-bind/splicing_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48028">
        <property type="entry name" value="GLYCINE-RICH RNA-BINDING PROTEIN RZ1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48028:SF4">
        <property type="entry name" value="SC35-LIKE SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="140"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="237"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="313"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="328"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4372119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="337" mass="36800" checksum="9C31905B07674841" modified="2023-11-08" version="1">MSKFLLRRTAARALSATPSKALLAKPRSITTLTCTAFRSRQQQWSIAAFQRRFASDEAAKTEAQAAVADAPENFAQTAAEAPVEDNLTPAQQEAQAEPTDASATVGTDALAAAAQAAEAPAKPTEQKRALRDGTPPQPNTTLYIGNLYYEVTADQLKRVFSRFGEVDSVKVIYDNRGLSRGFAYVEFKNIPDAQAAVDNLDMQVFEGRNLVVQFHRAKPNSRTRAPSGSYETNAPSRTLFIGNMSFEMSDKDLNDLFRDIRNVMDVRVAIDRRTGQPRGFAHADFIDVASATKAREALKAKVIYGRQLRVDFSKSGTQSQREKRADDAGNAGNAGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCB5</accession>
    <name>A0A9W8YCB5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Nucleolar complex protein 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NOP14</name>
        <name evidence="8" type="ORF">N0V83_004163</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCB5"/>
    <dbReference type="OrthoDB" id="441771at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007276">
        <property type="entry name" value="Nop14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183">
        <property type="entry name" value="NOP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04147">
        <property type="entry name" value="Nop14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="68"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="267"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="420"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="748"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="204"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="218"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="305"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="372"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="420"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="750"/>
            <end position="771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4372389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="842" mass="95062" checksum="74E0373938E3F06C" modified="2023-11-08" version="1">MPPSQLKRLKASLREQGITGPQKSKKQKKAQNKNADHRAQKASALASIRESFNPFEFKHLARPKKFDYVSNQPENATKKILGRPGVTKSQGEETRRKTLLPEMNRKNKVGGILDRRIGENDPTMTLEDRMMARFEREQQRKRGGNVFDLEDGDDEVMLTHGGQSLRFDDEIGEEDYDAASVSGSSDGEDGEDGFLKKKRRREEVDGEEDGDAPEEEQPEKKKSKAEVMKEVIAKSKLHKYERQAQKEDDDELREELDKDLNDVLAALRGHINKKPEPEAPKEATNNDFGINADRAALLAGSSQQAKDKEYDVRVRQMLQDERAKPTERTKTEEEKAREDAKRLKELEEKRLKRMRGEPTSDDEEPRKKGVENDDEDDDIESEHDDAAEFGLGAAPGQFSRPEGVEDEDDFIMDDDLIASDSEADMSEDESEAGSAPDDTMAMDDDDADFLKNVLPEQKEQPKAVNGVLTLGATTTSSKLAFTYACPRSHEELLTVFKNVAPSDTSTVIQRIRALYHAGLRADNKNKLADFACALIDHVSYLSKQTPAVPLAVIEAVIRHIHSMSRSYPVQIATQFREHLKQLQASNEPTTGDLAILTAIGSIFPTSDHFHQVVTPAITLMARWMGLTTPSSAKDIATGAYIGGMCLQYQKLAKRYVPELIRYTALCLKSPHATPALLTAHTKNILTAAELWSASPAFIEIFEPLLAPLKSAKQASTLQNLRARLALARSRRRPQLLHNHRPLPIKSSIPKFEESFDPNKHYDPDKERSEAARLQKEYKRERKGALRELRKDANFIARETLREKKEKDAAYEAKYKRLVAEIQSEEGREKNVYDREKKKRQRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCH7</accession>
    <name>A0A9W8YCH7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002598</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCH7"/>
    <dbReference type="OrthoDB" id="3786709at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="360" mass="40498" checksum="45A1BFB32B0927BE" modified="2023-11-08" version="1">MSSHQDRNYSGTSEGIDEQHEPLALEAVPSLINSTSLNDAQKEAILIKCGHHTLLRFYDQAMASLAGLLPLKSGDLNETNYRPLVSESEVPPALDAPLPLYPPYTGYFQSSQDAKTYRKRIRVAPKANAPDVERVKRSGRQYWVPRIYEAMIDITAVMDRDTSIHRRRFTASADFDPTDLEAAAHHVFDAALAVHERGFNRPQIYHKKVVRGKLMDVSEKSLEKRLARICLCLKHVKATVDDAVRGGVTLALLCDNPEARGFTKWSNNVGNKKRGERLRLAKEAAKKVAAKKVAAKKAATKKAKQQAEQQRVVHDDEKEDEDEEDEDEDEDDDDDVSGDDEEDVDEEDDDDEEDENEDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCY6</accession>
    <name>A0A9W8YCY6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ZN622/Rei1/Reh1 zinc finger C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003302</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCY6"/>
    <dbReference type="OrthoDB" id="19329at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041661">
        <property type="entry name" value="ZN622/Rei1/Reh1_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040025">
        <property type="entry name" value="Znf622/Rei1/Reh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13182:SF8">
        <property type="entry name" value="CYTOPLASMIC 60S SUBUNIT BIOGENESIS FACTOR ZNF622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13182">
        <property type="entry name" value="ZINC FINGER PROTEIN 622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12756">
        <property type="entry name" value="zf-C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZN622/Rei1/Reh1 zinc finger C2H2-type" evidence="2">
        <location>
            <begin position="78"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="342" mass="38877" checksum="DCE9C2AD84DE6461" modified="2023-11-08" version="1">MASKPHTFPRRVAGLPALSEEQYDTQAETRDSVYRQKDSDEQKKKRMPSPEAEVEPDFPLEDITTVKHKISEKIASTQCLFCNLDSPTLDANVDHMSSLHGLFIPSPDQLSDMESFLGYLAVIIFEYTKCLYCGSEKGTVDGVQTHMRDKGHCMIKMNTDPELLDFWELSDNGDEGQNEDEERIECAAIKLSDTEWRLPSGAIINARSDTTHFRSRPGIAQLRTKSSQYRTKRDELRAITAGESQETDEKQTKPASSNDRRVAVRGEMGLAGVSESQRRALQITAKKMKQREAIAKTANRHAMEQQPVRTMYYKVRVYDEKIWSNECSLMHEQTENPVYQAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDI6</accession>
    <name>A0A9W8YDI6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002884</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDI6"/>
    <dbReference type="OrthoDB" id="408373at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="28"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="166"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="335" mass="36307" checksum="B36741209AA99E88" modified="2023-11-08" version="1">MSKIPNSLMATSRLKVPAPYLYVAIGAASLDLFGRGYSAAPCPETHDYDSSLYMSQILLCLQSSPISWSPFTIVGYSLGGALGADFTSYFPALIKGLVLVAPGGLIRDKHISFSSKLLYQTPSLLPETLVEKLVAKRLWTGSKPTSNSNNNNAVEPQPGSVENAETTTTTTTTTARPSSSSSSKDKSTNAAVYLSSTHLLLPHNPASTVSAVVDWQLTHHAGFVPAFISTIRHAPIHNQHARWRAIREHIEKKNTELKRVWMVLGETDPIIIADELLEDAVGVLGEEIARWRVLEGVGHEVGVENADDIVDVVVRKVLGKKVKSTRAGRARSHHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDK7</accession>
    <name>A0A9W8YDK7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001927</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDK7"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="364" mass="38485" checksum="A0923E914B89D32B" modified="2023-11-08" version="1">MSSSPERIQALRAQIRPLVRAKPLYPKLTAFRNDIFQQLDSLVSRKRYNWPHINGYSRLNVKHALAFRLAMDLYNEGGIQMDVQPGTSGQMLVVAPTTPATMPVVMPAMSSTMPIVTPATMPTVTPATILATTPATTPATMTPMTLRSHVLPSESTRDIPGAGSTGDIQGDEGDYGPPITPSDLDAMHGLGDDDLSGVYVPASTQQSSMHTSSPSKRPTTEKVFIQETSPSILKDGLGSIISMDLMAAAPANARRADPSIPYRAAAPVLEAAAALPVAVPEPEAWLVAVAVAVVFMPELAGAAVLIDDPVALALPPESRFAPSTTVAVANVTSLLDKTAVTGPLVSSFPPDWFTVQVISTAWEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDN0</accession>
    <name>A0A9W8YDN0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribophorin II C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_002944</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDN0"/>
    <dbReference type="OrthoDB" id="432292at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056790">
        <property type="entry name" value="Ribophorin_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008814">
        <property type="entry name" value="Swp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640">
        <property type="entry name" value="RIBOPHORIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25147">
        <property type="entry name" value="Ribophorin_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044247588" description="Ribophorin II C-terminal domain-containing protein" evidence="8">
        <location>
            <begin position="22"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II C-terminal" evidence="9">
        <location>
            <begin position="181"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4374203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="289" mass="30518" checksum="68C74AE2F8C7B444" modified="2023-11-08" version="1">MRVLYSVVSSLLLSGATIVSAASSWSFEDATVSIASKGAGVGGGSKDKLNPSSPLSKSVSLGATDTLKLLLTTVDGKTAKRPHQAFLTLTDPTTGLEESFVLSVKESGKGKVDLSHKDLPHQFLTSTKPITASIVIGAFGSSTPYKSKVFDLQVARDPNVPLTMPEAPLRYAADSEIHHIFRTDPRSPAKIITIVFAAAVAAALPVLFGVWATLGANASHLNKALGNAPVSHALFYGSILAMEGIFFLYYTSWNLFQTLPAAAIVGFVAFLSGSRALSEVQERRLAGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDP6</accession>
    <name>A0A9W8YDP6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fumarylacetoacetase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_004182</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDP6"/>
    <dbReference type="OrthoDB" id="74910at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018773">
        <property type="term" value="F:acetylpyruvate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.850.10:FF:000003">
        <property type="entry name" value="Fumarylacetoacetate hydrolase domain-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820">
        <property type="entry name" value="ACYLPYRUVASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820:SF7">
        <property type="entry name" value="ACYLPYRUVASE FAHD1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="3">
        <location>
            <begin position="13"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4372408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="234" mass="26099" checksum="ED987EBEC5BA96C3" modified="2023-11-08" version="1">MASIQSIRANCRKIMCIGRNYADHIKELSSATPKQPFFFLKPPSSILLPGAGPVLRPRGVNMHYEVELGLIMGKTLRDLHPEDTKGAMDAIEGYVLAIDMTARNVQDEAKKKGLPWSIAKGFDTFLPISNFIAKHRIPDPQKADLWLSVNNEVKQSDNTELMLFRIPRQLSDISRVMTLEKGDIVLTGTPKGVGPVKTGDIMRAGLRVDGKDIEEANLEVPVEDREGLYEFRET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDR8</accession>
    <name>A0A9W8YDR8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001901</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDR8"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4374824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="121" mass="13614" checksum="FC61D78F2CF9A4D2" modified="2023-11-08" version="1">MQTLKIKDSTEARARNEIAELHQQNLKTIKEGRFSVRLEMMQAEKASTQPQPIKIEEEDADVDVQDHGFDETTLGNADQEPEAWNNLADDDVVVLAVDEDEDAGGTGEDRKAFIKQFVQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEL6</accession>
    <name>A0A9W8YEL6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEL6"/>
    <dbReference type="OrthoDB" id="3560543at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4375780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="215" mass="25434" checksum="04DB546806C77824" modified="2023-11-08" version="1">MPGSKPPTFAISDRYGPSIDPKLMPLARDLFSFDLELLRRVERKHASSSDEIRKNRAVPRVEEIFEIRQQWQDIELCRSQFSQSYVQDKLFIPVWVEGLRIVKEALRNNHVSGNHMKQRCDYMNILARYEKLACRLRDRLVDTARQGLLTNEVLMSTQERWIRLDGLLDEYHDTYNDHRDEDTSFAPRIAPKWSVQDCKMRMETLIYVIGKKFDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YET1</accession>
    <name>A0A9W8YET1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="9">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">UBR1</name>
        <name evidence="12" type="ORF">N0V83_002184</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 9">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="26387at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16482">
        <property type="entry name" value="RING-H2_UBR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19673">
        <property type="entry name" value="UBR-box_UBR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.30:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase UBR2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044046">
        <property type="entry name" value="E3_ligase_UBR-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055194">
        <property type="entry name" value="UBR1-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF24">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18995">
        <property type="entry name" value="PRT6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22960">
        <property type="entry name" value="WHD_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="11">
        <location>
            <begin position="85"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="8">
        <location>
            <begin position="85"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="404"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="468"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="518"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="411"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="432"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="472"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="521"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4375101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="2161" mass="244005" checksum="D48372F7E83A8105" modified="2023-11-08" version="1">MYISLQEQELCRHLRDLPRRHNNRFELAADRELREILFRSLAGRSEYLPLLFPGGPPTDTDKPWSLRDAQGAVEGAEYTAAARGKPCGHIFKNGEATYRCKTCTADDTCVLCARCFDASDHEGHQVFVSVSPGNSGCCDCGDDEAWVRPVHCNIHSADPEPESKAAGKAKAGAILPDDLLESIRITISRTLDYLIDVFSCSPEQLRLLKTAESITQDERNSRLTSRWYDPGDQPEECQEYCLVLWNDEKHTVNDVRDQVARACKQKLAFGLAKAYEVNDVGRSAVVYRTELKELLRMAKIIEEIKLTVTIRSARDTFREYMAGAIVEWVSDIAGCSVGDDNQVLRRTVCEELLKPWRVGSEASNQIIGKSGLDDHEMEEREAEMDGFYHGLLPIQRQTRIVRIQRRRAADPNDDTTASDDDDEEGGQGAADAGDDMDIDEVGAQDNDGDVEMEAYESPDEALEVSEATMAGYPPPPPPPPPGPPQRRHTEQSVTPAESDSEPMVGAPSLAHVEPLMPVPETPLTRSLPLRPSRPPKHWLEKPDSYGRKPGTLAHEDLWQRLRLDHLILYDLRMWKQLRIGVRDVFISTVVTIPQFKRLLGLRFAGVYTALAQLYLIADREPDHSIINLSLQMLTTPSITSEVVERGNFLTNLMAILYTFLTTRQVGYPSTVDPKATLAFEQGAVTNRRMYHFFLDMKYLLGSDFVQERIREEERYLLQFLDLVKLHQGICPNVRAVGEHLEFESEAWISASLITREINKLCRHFAESFAWNRDDDPTNIRRAIRAAAKVVIVNSLGAERKRFDQAELKTETRFKSLDVLEFDAEPSTFFGPSYKIVDYVVAKEPMSFHHALHYTLSWLLDRARSMPREVLLQLLQFSHGELQVDFNSTGVSIPIHQPEEYLLAMFDFPLRVCAWLAQMRAGIWVRNGITLRHQMTQYRSVSQRDVSYQRDLFLLQTALVLCNPSVFLATMIDRFGLTGWMTGKYEASQHGFEDSQAIDMVEDFVHLLIIILSERTSAVPAEDNKESHLTSMRRDIAHVLCFKPLSFSDMTARLSDRIQNMDEFPVVLSEMTRFRAPEGLSDSGTFELKEQYLDLVDPYLYQYNRNQREEAENIYKTYIAKKTGKQASDIVYEPKLRPIPSGLFQNLSAFTRTSLFTQIIYYLLGYGLRAAAVTPNVPATRVETYIQFVLQLVLVAVLEDKTEEHEWSQDAPDSFVTSVLTKHANMGIHDHPTVLSLLKALQEKEAFKACEPKINLILHRLKQRQQNTFIIAAAALNIPTDRVDNASPASFALQDKELKKKQALERQAKVMAAFKGQQEKFMANQDFDWGEDDFSDLEDETGGLVEQEKTLKYPAGTCILCQEDCNEQRLYGSFAYVSESRILRQTDLQDDDWVDEIVQTPVSLDRSADELRPFGVAGKNRRMIEKVASSGERLASERQDLGKGFPRLHVKPGPVTTGCGHIMHYACFEVYLQATQRRHVSQIARNHPERPEFKEFMCPLCKALGNMFLPIIWKPKKVSHPGVLDTDSSFDNWLESQLVAVYKSAERGTGKAPADLSLDQRVLYDYAERSFIQPVASHLPDLLKPPLPALSMPLPQPSTQVAPRFQIPAFLGIQSPDTGETVLMSSETSPALTQNFPMAELVKIYQRLNATLNANSIHSTFEYPHTPATAVEDLTHSDVLARGLGYSIAAAEIAQRGVQSEFVRGTLVDRISPQTMTHLRVFSETVSSYIAIGGLRHQSSRRPVDEFSQSQRRQFRQLFVGHPGIFDAEVLSFDLKGIAPILCEDPFIFLTECAVGVVPAMNIDVHHILRLCYLAEIVRVVFTFALAVDEQAPEGTALHPQLRFQHPEHFINALKVNNGFFPPGQLNDILFFVCVVFNVANMGDSPASLDIDNMPIPARFKDPNFLYFIHNMVSTYALPFVRKAAILMHVSYGIELPFVPLEHVDEPELARLSSLLKLPSLDEIFSSFRAHSTSGHTTRSVVGGWIRHLLWVQAGNVPLKPTISLSHPAIFELVGLPKNYDTLTDEAIRRRCPTTGKELTDPALCLFCGEIMCSQAVCCMTDRHRGGCNQHLAKCGGNIGLFIHIRKCMVLFLNLDHGSWTVAPYLDKHGEVDPALRRHHQLFLNQRRYDALLRKVWLEGGIQSLIARRLEGEINNGGWETF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFY6</accession>
    <name>A0A9W8YFY6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6594 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000057</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFY6"/>
    <dbReference type="OrthoDB" id="5342093at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046529">
        <property type="entry name" value="DUF6594"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34502:SF5">
        <property type="entry name" value="DUF6594 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34502">
        <property type="entry name" value="DUF6594 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20237">
        <property type="entry name" value="DUF6594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6594" evidence="2">
        <location>
            <begin position="14"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="296" mass="33021" checksum="B097DB424AF06FD5" modified="2023-11-08" version="1">MASKDPFPGIVAGYPKLAAKIEIQPEVAIYRRFGGLSALNLLYFQAELTDLEKKLWAQQVQDHNNTKGSKSEYAKNWFFLSDSAYDGDTEQLDLVMRMRETLKEYSESKLQAERALTLLTRCVDHAIIQQAVILNYPEPGKWDLHHLQEYLQTPEMGSLLALTGADARVWGSMAKPKSHAPDLITLRPRAKKDPFSVWAAENTIFNLLKCGCARFMKPSRVHGVVGYEDGTIYRITYGITNVLASLIPIVSIVVLYRVHSMPAKLGIIAAFNVLVSICLMAFANAKRAEVFAVTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFZ1</accession>
    <name>A0A9W8YFZ1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Small nuclear ribonucleoprotein Sm D2</fullName>
            <shortName evidence="12">Sm-D2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 12">snRNP core protein D2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SMD2</name>
        <name evidence="14" type="ORF">N0V83_001578</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the snRNP core protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFZ1"/>
    <dbReference type="OrthoDB" id="437526at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01720">
        <property type="entry name" value="Sm_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000018">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027248">
        <property type="entry name" value="Sm_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12777">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="13">
        <location>
            <begin position="35"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4376295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="117" mass="13423" checksum="6771E6EC7EA3B514" modified="2023-11-08" version="1">MVDAKIQELLAKPRAELTEYEVAQVEEHELTTGPLSLLQTAVRTRTQVLISCRNNRKVLARVKAFDRHCNMVLENAKEMWTETPRLANGQRGRKVNKDRFISKMFMRGDGVILVLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGF1</accession>
    <name>A0A9W8YGF1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carboxy-cis,cis-muconate cyclase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001868</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cycloisomerase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGF1"/>
    <dbReference type="OrthoDB" id="1715191at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000244">
        <property type="entry name" value="Carboxy-cis,cis-muconate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050282">
        <property type="entry name" value="Cycloisomerase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019405">
        <property type="entry name" value="Lactonase_7-beta_prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30344">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30344:SF4">
        <property type="entry name" value="CYCLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G11580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10282">
        <property type="entry name" value="Lactonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75011">
        <property type="entry name" value="3-carboxy-cis,cis-mucoante lactonizing enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="40921" checksum="B51957C954D6CE1D" modified="2023-11-08" version="1">MVKHHLMIGTWTPPGVIITVAFDDETLKLELVKKTEIPEDEPISWMAFDHQRKTIYGASMKKWSSHEVKSPSEIVHTGSFPIGGHPKASDADTKTRAIFLLPAKKPPYAVYCNPFYDYAGYGNVFSVNESGHIKQNIQNYEYCPQTAIHGMVFDPSETYLYSADMWANRIWCHKKIDDEGRLETVGYTEAPAPKDHPRWVEMHPSGNYLYALMEAGNRLCEYVIDPQTKLPVYTHKTFPLIPPGIPNAKTMYRSDVCFLTQSSQYLFATSRSNSFSLTGYIAAFKIAPSGAIERQICLNPTPTSGGHSNAVSPCPWSDEWLALTDDEKGGVEIYRWHDEFLARVARLEMGEKGFGMNAIWYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGR8</accession>
    <name>A0A9W8YGR8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Altered inheritance of mitochondria protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">AIM6_1</name>
        <name evidence="4" type="ORF">N0V83_000630</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIM6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGR8"/>
    <dbReference type="OrthoDB" id="4153866at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08577">
        <property type="entry name" value="PI-PLCc_GDPD_SF_unchar3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039559">
        <property type="entry name" value="AIM6_PI-PLC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051236">
        <property type="entry name" value="HAT_RTT109-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4377800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="394" mass="44530" checksum="CE86B8FDC2B24304" modified="2023-11-08" version="1">MEQKDENSKSKSQIVVAVRPVYPRMLSGDGRPRKFRDDEDETSSQASSAISLEEGNINRRKPKCVYISFVTTLIRRLAPPPGHSGLQHIVETWKEPDSNGAFKFDWRDDISRDIVPKNCHSHNDYWRRVPLYEALAAGCVSIEADVWLTEDKELLVSHSWQSTTKERTLRSLYLDPLTNIFENRNVSAASTDDKQTGMFDSDPNTSTILLIDFKTDGHELWPVVLSQLQPFRDKNWLTYYDGEKLIQGPLTIVGTGNTPFDLVQQNSTDRFIFFDAPLLSLSSSSDGDKYNTTNSYYASTNMKAAIGRIWPHTTHKLSSKQVDTIQSQIKAAEDKGLKSRYWDTPPWPIALRDNVWSTLMDAGVGMLNVDDLVSASRWNWGWCVVAGIALCGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGV4</accession>
    <name>A0A9W8YGV4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGV4"/>
    <dbReference type="OrthoDB" id="5151921at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="299" mass="33405" checksum="2E751FA245B5404B" modified="2023-11-08" version="1">MAKSKLRAISGPMDAKHVGGVSVTGSNSSSLDSYFTNTTIEPDELPSHTFVAKGTTELPRRSDTIASVIRRPSISLKRSLSRLRTKSISHLPDLHRKHESEQQADKPVTRSDSTKASRPLRMQSSMSRLRQKVGLDREIYEPPVPKSTTPEPDIAPEPVPKDYPPLRPRRSLARLTTASSIYPTVPDSDLARTSTIIQRQSSTVQRRPSPPIQKESSITQPRKQPPVRPKRADSGTAIEFNDVPMDERPLGFKEILAVQTFAERMALYKKTREYWASADHGLMEWTGRVGAPRLVPTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGX9</accession>
    <name>A0A9W8YGX9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_000138</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGX9"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF189">
        <property type="entry name" value="DRUG TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G03920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="40"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4377313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="174" mass="18418" checksum="DD4CC86B11C03ED3" modified="2023-11-08" version="1">MADSKTEITPNGMIIEPTSNETLSSEAQNGPIFNEQTDYIFLACASVDLLALMDQTTLAASLTIVSKSLAASSSSGWIAGAYFLTSTSFQLLYGRLSDIWSRKVILMVGIAIFFVGSLAASLAQTAVQLIAFRALTGVGGGGIMTVAQFIVSDIVPLRERGKYQGILVRGKEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGZ4</accession>
    <name>A0A9W8YGZ4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Metalloprotease</fullName>
            <ecNumber evidence="11">3.4.24.56</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">STE23</name>
        <name evidence="11" type="ORF">N0V83_000024</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.56" evidence="11"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGZ4"/>
    <dbReference type="OrthoDB" id="952271at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000003">
        <property type="entry name" value="Insulin-degrading enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000005">
        <property type="entry name" value="nardilysin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000004">
        <property type="entry name" value="Putative insulin-degrading enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032632">
        <property type="entry name" value="Peptidase_M16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054734">
        <property type="entry name" value="PqqF-like_C_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16187">
        <property type="entry name" value="Peptidase_M16_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22456">
        <property type="entry name" value="PqqF-like_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="7">
        <location>
            <begin position="39"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="8">
        <location>
            <begin position="228"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="9">
        <location>
            <begin position="412"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="domain" description="Coenzyme PQQ synthesis protein F-like C-terminal lobe" evidence="10">
        <location>
            <begin position="805"/>
            <end position="903"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4377201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1122" mass="127616" checksum="5E626614696177EE" modified="2023-11-08" version="1">MGETLGRSVGSRQRIEDLERPQLDDRSYRIITLPNQLEVLLIHEAGTDKASAALDVNVGSFSDAPDMPGIAHAVEHLLFMGTEKYPEENAYNKFLTGHGGYSNAFTAATSTNYYFELSYPSSTPTNSTAATPNASQIDLPQSKDESPLWGGLDRFGQFFISPLFLEDTVDRELKAVDSENKKNLQNDTWRLHQLNKALANPEHPYNHFSTGSYKTLHDDPIARGVKIRDEFIKFHSTHYSANRMKLVVLGRESLDTLEEWVEEIFSKVPNKDLKHNRWDMPVYTKNELLTQTFAKPVLESRSLELAFAFRDEEEFYESHPSRYLSHLIGHEGPGSILAHIKAKGWANGLGAGGSTLCPGSGLFSISLKLTEEGLKNYKEVAKIVFQYIGLVRDQPPQEWVVEEQMRISEVEFRFKQKSPPSRTASSLAGIMQKPYRRELLLSGPATIKKFDARLISEAMEYLRPDNFRLTIVSQEFPGGWDEKEKWYGTEYKIEKIPEDFLAELKEAFESKNRPAELHFPHKNEFIPSRLEVEKKEVQQPSKWPKLIRNDENVRIWWKKDDQFWVPKANVHIYFRTPITHVSARVTLLCTIYRELVNDALVEYSYDADIAGLIYEFTNHISGLSLAVSGYNDKLHVLLEKVLLQARDLEVQQDRFDIIYDRMRRSLRNWDFGQPFHQVGTYSRQFKSEKSFMNEELAKELESVTAQDVQQFFPLILAQCQIEVLAHGNLYKEEALKITDLVERTIKPKKLPATQLPIRRNLIPPTGSNFIYEKQLKDPANVNHCIEYSLYTGSRYDTDMRAKLLLLGQMTDEPCFNQLRTIEQLGYVVFSGSTFADIWAGYRILIQSEKDCRYLEGRIEHFLQTFEETLQEMSEEDFESHKRAIINKRLAKLKNLSQEDNRFWNHIYSDSYDFLQGTSPPSQAPIPGDEKIANYVTADTDAATIEKLSKKNMTDFYAHYVSTSSSQRSKLSVHLQAQAKAKEATLDETKAAAVVALKIILAEHKITPPDDADATLQSRIDNATSTEAIPAAVAVFLNEDLTLAEDVSAKVLDEAKAALGVADSGLPAEPQPLDEKADIESVVDASHPVLITDVHAWKASMQVSSGVRSVRNLEEFIEVAEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH32</accession>
    <name>A0A9W8YH32_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein fmp52, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">FMP52</name>
        <name evidence="7" type="ORF">N0V83_000795</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FMP52 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH32"/>
    <dbReference type="OrthoDB" id="430436at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051170">
        <property type="term" value="P:import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000366">
        <property type="entry name" value="Protein FMP52, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014843">
        <property type="entry name" value="Him1/Fmp52"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14097">
        <property type="entry name" value="OXIDOREDUCTASE HTATIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14097:SF7">
        <property type="entry name" value="OXIDOREDUCTASE HTATIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08732">
        <property type="entry name" value="HIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4377965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="227" mass="23954" checksum="DAE5E1E753B4B0BD" modified="2023-11-08" version="1">MATATLAGSTGLVGSNILTQVLASQSFSAVYAYTRRELPNPTAATKLNPIISTDNAQWPSLFPREMKPNILFSALGTTRATAGGIEGQRKIDYELNLELAKAAKDAGVETYVLISSGGANSKSSMAYPRLKGELEEAVKALEFKHTVILRPGLIVGDRSESRPAEAVVRGIAKGLRSLTPALTNFWAQDASVIARAAVNAAVQCVEGKKEAGVWELGQADIIALGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YI97</accession>
    <name>A0A9W8YI97_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001394</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YI97"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4376113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="63" mass="7237" checksum="57D3FAACF0D72B0E" modified="2023-11-08" version="1">MGSGTYTYQNTITDEYDAEKREGIKWTRKTSIICEGSGNADDKDGEDRDWQIQELCRGDIRES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJT1</accession>
    <name>A0A9W8YJT1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Inositol-1-monophosphatase</fullName>
            <ecNumber evidence="7">3.1.3.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_000650</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>a myo-inositol phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24056"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84139"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 6 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJT1"/>
    <dbReference type="OrthoDB" id="10254945at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01639">
        <property type="entry name" value="IMPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000004">
        <property type="entry name" value="Inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000012">
        <property type="entry name" value="Inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033942">
        <property type="entry name" value="IMPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854:SF4">
        <property type="entry name" value="INOSITOL-1-MONOPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="289" mass="31450" checksum="96FD05C5EB3D46CD" modified="2023-11-08" version="1">MADINLEEIHDFMISIAKQAGERIVSAKPTTENAGSKKNSVDLVTETDRAVETLISTSLREKYPSFSFMGEETYKPGDKITSAPTFICDPIDGTTNFVHRYPYVSISLGLAIDLEPTVGIVYNPFTQTLYSAIKGRGAYLNQTTKLPLSPPTPLETLNSCLVAVEWGSDRSGNDFKVKSETFKRLAATKEDGGGMVHGLRSFGSAALNLCGVASGGLDIYWEAGCWAWDVCAGWVILKEAGGVMVDANPGKWEPRVDERRYFAVRGGEGQKEIIEEFWGLVDGACEVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CGZ8</accession>
    <name>A0A9W9CGZ8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tyrosinase copper-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_010707</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CGZ8"/>
    <dbReference type="OrthoDB" id="6132182at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1280.10">
        <property type="entry name" value="Di-copper center containing domain from catechol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008922">
        <property type="entry name" value="Di-copper_centre_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050316">
        <property type="entry name" value="Tyrosinase/Hemocyanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002227">
        <property type="entry name" value="Tyrosinase_Cu-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474">
        <property type="entry name" value="TYROSINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474:SF126">
        <property type="entry name" value="TYROSINASE-LIKE PROTEIN TYR-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00264">
        <property type="entry name" value="Tyrosinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00092">
        <property type="entry name" value="TYROSINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48056">
        <property type="entry name" value="Di-copper centre-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00498">
        <property type="entry name" value="TYROSINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosinase copper-binding" evidence="3">
        <location>
            <begin position="157"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4361767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="237" mass="26521" checksum="DFD37E385247135D" modified="2023-11-08" version="1">MLRDECGYHGAQPYWDWTLDTPASKFIDSPIFDSVYGFGGNGPLIPVNASNNEVPGRTGGGCVKHGPFRNMTVHLGPFASLERNDQCLKRDLAPEYAAKFLSHDKLAEVLLHSDYGWFARTLEGGTSWDDSQIHAGGHFGVGGTYGQMGDPFASPADPIFWMHHANLDRVWWSWQKKDLAERLRDMSGPIVIQDWSNAQAGNVTLEFPLSLGYNNWDKTIKDVMDIGPLCYSYDELY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH16</accession>
    <name>A0A9W9CH16_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_010726</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH16"/>
    <dbReference type="OrthoDB" id="10259236at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02883">
        <property type="entry name" value="NUDIX_Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="44"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="80"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4361785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="260" mass="28893" checksum="B198B0A4D733036E" modified="2023-11-08" version="1">MRNPFRSKPAVPALPQSTSDDDMSTTSSTPRTFAPVIAQYAGENFVVGGGVAIFHIATERVVICSAHDRRGTKYYFLPKGRRDAGEESGRGAEREGYEESGYRNRLLPLPTKHRQPQAHPRVHALPLTAEAVWMQLMPLGSRQYVLYWYIAETLPPDLEKELETEAGAAYKPPPPYPRNLPLRERIRMDPDGYEPLHHEGTGVDEEEQTYESHLVSVEEAISKLGRGGVMADVVLKGWNGIQDRLAIEDAATTESPEATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH32</accession>
    <name>A0A9W9CH32_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Succinate dehydrogenase assembly factor 2, mitochondrial</fullName>
            <shortName evidence="4">SDH assembly factor 2</shortName>
            <shortName evidence="4">SDHAF2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">emi5</name>
        <name evidence="6" type="ORF">N0V83_010860</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Required for flavinylation (covalent attachment of FAD) of the flavoprotein subunit of the SDH catalytic dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with the flavoprotein subunit within the SDH catalytic dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SDHAF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH32"/>
    <dbReference type="OrthoDB" id="284292at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.250:FF:000002">
        <property type="entry name" value="Succinate dehydrogenase assembly factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.250">
        <property type="entry name" value="Flavinator of succinate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03057">
        <property type="entry name" value="SDHAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005631">
        <property type="entry name" value="SDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036714">
        <property type="entry name" value="SDH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028882">
        <property type="entry name" value="SDHAF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12469">
        <property type="entry name" value="PROTEIN EMI5 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12469:SF2">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03937">
        <property type="entry name" value="Sdh5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109910">
        <property type="entry name" value="YgfY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="89"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="212"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="89"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="231"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4361919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="314" mass="34834" checksum="1226E561C742E14E" modified="2023-11-08" version="1">MASSRLVARSSRALLTVQRTQCRAFSISTTRSNEFEALNKRTNDTSETYRKFQIEKPLNPHLTNTTSTIANEMPSVGKDAAPPELIKHADKEFTPKDSVPKNTERMTGGTQKSEGADVSGVNKELGVGEMEGAKFKVEPLRRTGEDPNTMRARLLYQSRKRGTLESDLLLSTFADAKLATMSPELMQQYDIFLDENDWDIYYWATQEPSQTSLETAEGSGSELATSNAQGKDAKVQEKDEVKRQPGTGEWAQTVGTFKPAYRPVPSRWKGSEILSLIRRHVQERKAGGVVEGEGGVKQEGKGLGMMPPIKNFDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH43</accession>
    <name>A0A9W9CH43_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">40S ribosomal protein S3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RPS3</name>
        <name evidence="10" type="ORF">N0V83_010858</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH43"/>
    <dbReference type="OrthoDB" id="10248446at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02413">
        <property type="entry name" value="KH-II_40S_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1140.32:FF:000004">
        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000006">
        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="entry name" value="Ribosomal protein S3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057258">
        <property type="entry name" value="Ribosomal_uS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_uS3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_uS3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_uS3_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003219">
        <property type="entry name" value="PRK04191.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760:SF32">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="Ribosomal protein S3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="KH type-2" evidence="9">
        <location>
            <begin position="24"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="217"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="231"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4361917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="266" mass="29193" checksum="CAD0B0CAE05508B2" modified="2023-11-08" version="1">MATPQSAISKRRKFVADGVFYAELNEFFQRELAEEGYSGVEVRVTPTVTDIIIRATHTQEVLGEQGRRIRELTSLIQKRFKFPENSVSLYAAKVQNRGLSAVAQCESLRYKLLNGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRAARAKSMKFTDGFMIHSGQPAKDFIDEATRHVLLRQGVLGIKVKIMRGSDPEGKAGPSKTLPDSVTIIEPKEEQPVVQPMSQDYGAKAIAAQQAAEQARQAEQGEGEAQPASGEGYSEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHX4</accession>
    <name>A0A9W9CHX4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondrial K+-H+ exchange-related-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHX4"/>
    <dbReference type="OrthoDB" id="5562676at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018786">
        <property type="entry name" value="Mit_KHE1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28062">
        <property type="entry name" value="K+-H+ EXCHANGE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28062:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10173">
        <property type="entry name" value="Mit_KHE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="37996" checksum="69F71F860A4236F9" modified="2023-11-08" version="1">MRLFLLPISTRRSLIYCEKLHEKAPADRSLYDKVTIKANDTWLAWEKDDKAIGNWKNKVTFYGNQALKRIPYEEWGLKTIPALTAKRKQDIIDGKAKYEVLFPGHYLPKERVPGLLEKLAKDRQNMHRSKLIWSVVIMPFTAPFMLIPVIPNLPFFYVLYRAWSHWKALTGSKHLEFLLKHNLPTPAPSAILDEAYTAGLMYPTRAISRAAPRPTLDQVQSVSQVVHQQTNNETEDVMVLQRWNGKLIAEQFKLPEMEVEIERAVEQVEKSIKTKEELMEEKLELEKAAGNGGERAKDDLPDHILGRIHQQAEHVAEEKEAADKAKNKASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIA1</accession>
    <name>A0A9W9CIA1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Chorismate synthase</fullName>
            <ecNumber evidence="4 8">4.2.3.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ARO2</name>
        <name evidence="10" type="ORF">N0V83_008794</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21020"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="EC" id="4.2.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>FMNH2</name>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
        </cofactor>
        <text evidence="8">Reduced FMN (FMNH(2)).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the chorismate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.5" evidence="4 8"/>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIA1"/>
    <dbReference type="OrthoDB" id="1721239at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004107">
        <property type="term" value="F:chorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07304">
        <property type="entry name" value="Chorismate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.150.10:FF:000004">
        <property type="entry name" value="Chorismate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.150.10">
        <property type="entry name" value="Chorismate synthase AroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00300">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000453">
        <property type="entry name" value="Chorismate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035904">
        <property type="entry name" value="Chorismate_synth_AroC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020541">
        <property type="entry name" value="Chorismate_synthase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00033">
        <property type="entry name" value="aroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003793">
        <property type="entry name" value="PRK05382.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085">
        <property type="entry name" value="CHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085:SF0">
        <property type="entry name" value="CHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01264">
        <property type="entry name" value="Chorismate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001456">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103263">
        <property type="entry name" value="Chorismate synthase, AroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00787">
        <property type="entry name" value="CHORISMATE_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00788">
        <property type="entry name" value="CHORISMATE_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00789">
        <property type="entry name" value="CHORISMATE_SYNTHASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7 8 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="293"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4365176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="409" mass="43893" checksum="D6F2086E1F4043E7" modified="2023-11-08" version="1">MSTFGTHFKVTTYGESHCLSVGAIVDGCPPGMALTDDDIQPQMTRRRPGQSAITTPRNEKDRVEIQSGTEFGVTLGTPIGLRVMNENQRKQDYGNQTMDLYPRPSHADWTYLEKYGVKASSGGGRSSARETIGRVAAGAIAEKYLREAYGIEIVAFTNSIGNEFLFPPTPEHPTASTNPEYLKLVDTITRKRVDEFLPVRCPNEDVTRRMEDLVAKYRDAQDSIGGTVTCVIRNCPTGLGEPCFDKLEAKLAHAMLSIPATKGFEIGSGFGGTQIPGSIHNDAFIKAPAVPAGENPSANVPRPRLTTKTNNSGGIQGGITNGAPIYFNVAFKPAATIGQSQNTVTYDQVDGVLEAKGRHDPCVVPRAVPIVETMAALVIIDAVLAQQARQGARSLLPPLKGVVPVNGTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIB6</accession>
    <name>A0A9W9CIB6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">TIM50</name>
        <name evidence="17" type="ORF">N0V83_009461</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to direct preproteins in transit and direct them to the channel protein TIM23, and possibly facilitates transfer of the translocating proteins from the TOM complex to the TIM23 complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Mitochondrion inner membrane</location>
            <topology evidence="1 14">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the TIM50 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIB6"/>
    <dbReference type="OrthoDB" id="287041at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000019">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit TIM50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10 14" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 14" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FCP1 homology" evidence="16">
        <location>
            <begin position="281"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="16"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="156"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="571"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="120"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="571"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4364006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="599" mass="66567" checksum="E02A02E1FC2BB12F" modified="2023-11-08" version="1">MLPRAAFRALRIRPTLAAPRISPIASTPQWPRAYASNNRRKDNTWKQSDPIKFDEKNKARPNPVNPSEQPEFDTAASPESNTTPSAPSRPVASATQASRDAGKHSAASGQEDFSGPKSPAENTDPTTESPESQQPQKPLPDLRQGIPSTFAAEFAEAEAKAKRDPNEPNEFPEDPTPGPSGGREGGGELPKSAYETSTDRRRNRVANWSYLAILLFGTTGAVFMGRNWESEEEELAHADAPNGWTLGQMYARAAARINGQKAYYTEPTFQKLLPDKDKIPGGAPELTLVLSLEDLLLHSEWSTKHGYRLAKRPGLDYFLRYLSSQYELVIFTSVKSMDADPIIRKLDPFRLVMWPLFREATRFEKGEYIKDLSYLNRDLSKVIMIDTDPSHVKLQPENAIVLPKWKGEPGDKGLVAMIPFLEYLAMMSQTGTPIDVRQVLKSMEGKDLPAEYARREAKLREAFQRDIAEQKRKSKGSAGGMFMKSLGLDAPKQGMVLGDQNISQGMSEGKMMIDMYREFNRQNYQHLDKQIRENGEQWLKEMAEEEKKMQEAQLKDMKAGAFGWLGGNAAPASATVPSAASAPEAVTSHNAGVPPEQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIL0</accession>
    <name>A0A9W9CIL0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">High-affinity methionine permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_009599</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIL0"/>
    <dbReference type="OrthoDB" id="5982228at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000025">
        <property type="entry name" value="High-affinity methionine permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF382">
        <property type="entry name" value="LOW-AFFINITY METHIONINE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="376"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="442"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="476"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4364144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="533" mass="58613" checksum="D5D5A4AAD25ECA27" modified="2023-11-08" version="1">MATKADEKDAKGIRHADDETPSPPEYENASVQEDNIYVYADEQKLGYTGTVFVILNKMIGTGIFSTPSGIFAVTGSVGVSLFLWIIGGILTFCGLSVFLEFGLAIPRSGGEKNYLERVYRHPKYVATCVLAAQMLLLGFSSGNSLAFGRYVLFASGSDAPDGWAARGIAIACVTFSVGLHATAPKWGIRLFNVLGVFKVFILLFIVFSGFAAIAGHLKVEQPSNFDNAFHLEVGDGYGGGGAYGYCQALLRIIYSYKGWENANYVLSEIKNPKKTLAWSAPLAIGGTTILYVLANVAYFAAIPKSDLAKSEVIVAGLFFRNVFGNSAGARSLPAFVALSNLGNVLAVSFAHARLNQEFAKEGLLPWSRFWASNKPFNAPATALFLHWLVTVIVLVAPPAGPAYNFITDLYTYPGSWINGFVTAGLIYLHYNKRERWESPWHTYLPIAVVYLLANIFLALVPFIPPDGDWNVEGYPYYVFPVVGVAVLLLGVFYWVCWTRIWPKFGGYKIVSQRYEDAQGNEHIRYVKVYTKHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIW7</accession>
    <name>A0A9W9CIW7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Aquaporin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">AQY1_2</name>
        <name evidence="12" type="ORF">N0V83_008656</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>H2O(in) = H2O(out)</text>
            <dbReference type="Rhea" id="RHEA:29667"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIW7"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000024">
        <property type="entry name" value="MIP aquaporin (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF199">
        <property type="entry name" value="MIP17260P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="131"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="325"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="327"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="346"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4365040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="376" mass="40499" checksum="FD96B3B349F42467" modified="2023-11-08" version="1">MAIKNLLPLHKKDAHAPAASGEPKAPHLPRHQQTSFITNRWRNELVAAAAEFAGTFMFLFFAFGGTSVANTAATYSDAATAGQESGSITQAPNTSVLLYISLAFGFSLMVNVWVFFRVSGGLFNPAVTLGLYLIGAITAVRAALCFLAQMLAGMCAAAVIQALLPGPLNVSTTLGYGVSAARGVFLEMFLTSLLVFTIFMLAAEKHKATYLAPIGIGLSLFVAEMVGVFFTGGSLNPARSFGPCVVTRNFPGYHYIYWFGPLMGTLLAFGMYRIVKLVEYQTVNPGQDFDDHEAALFKPPSDPESAEQVERPNVAAAAVEEAIRMASRSSGSNDGPQNVYGIEQTISRDEEREREQRERDLLARTGHGPQANGVRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJH2</accession>
    <name>A0A9W9CJH2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosomal eL28/Mak16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_007495</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJH2"/>
    <dbReference type="OrthoDB" id="338850at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.110:FF:000002">
        <property type="entry name" value="60S ribosomal protein L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002672">
        <property type="entry name" value="Ribosomal_eL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029004">
        <property type="entry name" value="Ribosomal_eL28/Mak16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10544">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01778">
        <property type="entry name" value="Ribosomal_L28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal eL28/Mak16" evidence="5">
        <location>
            <begin position="9"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="71"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="77"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4366965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="150" mass="16317" checksum="1C3FFB1007355F0D" modified="2023-11-08" version="1">MAATIPHDLAWEVTKGHSATLVKRPNGVQFSRDPLNLRSVYSRKNEGQIANKAIGVIPGKDGGVTLLVKKSDKHHQPASSTQTTTFGSQRSTRKTYASIVNSTTKRNYRPDLRKDAVARASAIRKSQRPVKADKPSKTRGAKARAAAEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJH7</accession>
    <name>A0A9W9CJH7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008386</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJH7"/>
    <dbReference type="OrthoDB" id="3788853at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4365766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="266" mass="30464" checksum="0226635A6C95AE24" modified="2023-11-08" version="1">MNVERCIELHNEILRYGWVNSGHDVSQFETAAKPWMSFFGDDGLAILPDLAPELDLDDIDRILFKRMKRYPLVKRYPLDVVLDYWLNTIRIGRIVAVPKNRPDGPFEDRLGIHPWRHRRFYDSMLNETLDAFNKLVEAIEARLPQGSSSLNDDSIEYGLADADVLQIHEIAPGFAREFLACARTPRFKHIAPGLEVITTSTFSKQPFIPNPDEKANKHDVPAILLFYSKLNYDNSPDRTEYGAGPFGYPCHQITTLPTGLYLDRTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJI2</accession>
    <name>A0A9W9CJI2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Carboxypeptidase</fullName>
            <ecNumber evidence="14">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">N0V83_007525</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Extracellular serine carboxypeptidase that contributes to pathogenicity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential release of a C-terminal arginine or lysine residue.</text>
            <dbReference type="EC" id="3.4.16.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="14"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJI2"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF189">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0843">Virulence</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041015926" description="Carboxypeptidase" evidence="14">
        <location>
            <begin position="22"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4366995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="675" mass="74028" checksum="A3CB6C60DEFBE877" modified="2023-11-08" version="1">MSSSKFIFLAIFGALAALVDAQFPPTPDGVTILESHIEDGIRISYKENDLCETTEGVRSYSGYVHLPANALADLGVQNQTYEINTFFWFFESRKDPANAPLAIWMNGGPGSSSLLGLLVENGPCYVGADSNSTYLNGWSWNNEVNMLYLDQPVQVGLSYDTLTNITTNIDTGDVEVADFSSSSIPAQNNSFYVGTYASQNYNLTTRGTENSARALWNFAQVWFQEFPQHKPNDGRISIATESYGGRYGPAFAAYFQEQNEKIENGTWDEQGQTHVLNLDTLLIINGCVDRYVQWPAYPQMAYNNTYGLKAYNESRYEGVINDLYRSGGCLDQIEECRNVSLAYDPSNQGFNESVNAICEAAETFCTENIRDPYFDTDRNYYDMSVPGAASFPPPWYEGWLNQPHVQQGLGVPMNWSQSNSAVAKAFRDIGDYPRPGWKEDLAYLLEEGIKVTLVYGDRDYACNWYGGELLSLAIPYPNASSFAQAGYADVVVNDTYVGGQVRQFGNLSFTRVYESGHEVPAYQPETAYRIFQRTLFNMDIATGNISTLTTPDYATEGPSDVYGIKNEPVTDPGSQCYVLDRAQCTDEQWETVMNGTALVKNWIVVDANTSSLFPGLIGNGTAPGNGTALPSTLVPSATGSGGGLPEATGGASGSLHASMYGSMTLTVILVLAGMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJR4</accession>
    <name>A0A9W9CJR4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008449</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJR4"/>
    <dbReference type="OrthoDB" id="5371583at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42069">
        <property type="entry name" value="HYPHAL ANASTAMOSIS-8 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42069:SF1">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="222" mass="24743" checksum="2E20F7C99C3BE283" modified="2023-11-08" version="1">MKPMHFVGHNELAEYHNAPTFQDFEHLKVAQKDDAQLKARIRHLRLLSRILAFAISLAVLVPITMTLVKFLTTKNTYRDVITTSTGETASRTAWAHNSKTWPTFVYFGVAVVSALLDFGTVISYMFGGVEQANAASHVASVFSWIIMVGNFVVWTVAAAMYRVEKNRNGKSNDLWGWTCSAGARAIQKEFAGEVDFSRYCTVQHLDVCEGEVEEESEAAVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJS8</accession>
    <name>A0A9W9CJS8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA-binding protein, CCR4-NOT complex subunit Rcd1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rcd1</name>
        <name evidence="2" type="ORF">N0V83_006914</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJS8"/>
    <dbReference type="OrthoDB" id="1183224at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000014">
        <property type="entry name" value="Cell differentiation protein RCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007216">
        <property type="entry name" value="CNOT9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12262">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04078">
        <property type="entry name" value="Rcd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="373" mass="41420" checksum="FF38C3E81A81BC3B" modified="2023-11-08" version="1">MQHIFAHQHQYPGEWSQGHQQQTHHHIAQHGQLQAQAQAQAQAQAAAAAAAAAQQQHYNRIAGNNGTTIPGNNVVPRGPANDVQATDIGMTDENRRVLEWIAQLMKPQSREAALLELSKKREQVPELALILWHSFGVMASLLQEIISVYPLLNPSQLTAAASNRVCNALALLQCVASHTETRGLFLSAHIPLFLYPFLNTTSKSRPFEYLRLTSLGVIGALVKNDSSEVINFLLTTEIIPLCLRIMETGSELSKTVAIFIVQKILLDDIGLAYICQTYERFYAVGTVLSNMVNQLVEQQTVRLLKHVVRCFLRLSDNARAREALRQCLPEPLRDATFSSVLRDDAATKRCLAQLLINLSDNVVDAANNQQLMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKM9</accession>
    <name>A0A9W9CKM9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aspartate/glutamate racemase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_005826</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKM9"/>
    <dbReference type="OrthoDB" id="412093at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016855">
        <property type="term" value="F:racemase and epimerase activity, acting on amino acids and derivatives"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001920">
        <property type="entry name" value="Asp/Glu_race"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4368744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="254" mass="28169" checksum="934B773920405124" modified="2023-11-08" version="1">MGSMASDSELAPIGFLAVECFFERPPGDPFNERTWPFPLIRELVPGSKESQLVIKDQYDDAFYDRFVEAGKRLAERGAIGLMTSCGFLAMAQPRLAERLPIPIATSALIQIPSILAWLPKNQLVGIITYNGEQLGDLHMKQLSVDPTRVKIRGATPGGHLQNLVREKVPGYPKDELTQELVEATEALIKDCSAKGETIGAVVLECTQMPPFGPAIQEKLGVPVYDVYTMGMWFYSGLQRRSPSWWSEKEKKVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKP5</accession>
    <name>A0A9W9CKP5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichol alpha-1,3-mannosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ALG3_1</name>
        <name evidence="1" type="ORF">N0V83_006379</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKP5"/>
    <dbReference type="OrthoDB" id="5392202at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052999">
        <property type="entry name" value="PTS1_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28180">
        <property type="entry name" value="CONSERVED MITOCHONDRIAL PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28180:SF2">
        <property type="entry name" value="PEROXISOMAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4368024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="283" mass="30573" checksum="B9784DEB5C66C1E6" modified="2023-11-08" version="1">MSKLSPALKSLINAAHSRPGPVPAPRQIQSIYSKIEEEATARKLGRPSWLGISTAATMTMNSPESMVALYNSSTKSQPDGEKVAIAEFMREIGLKCIGFNGIPRTINMLNAFRADLPSSIASSLNTKPTRFPDPSNIESINTRGRALWNAIYRPLETKLEHKLGDAHPDLPVFIINNEYGGLFADPPREAGATVGRITTSLIAVTCLRAQQGVGPQVLSHVFGLRKAWEDGTWKQEPAAGEEEGIRWLVSDEGCAWVLEKVDELVAALGGGQGSTFAPVKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKW6</accession>
    <name>A0A9W9CKW6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKW6"/>
    <dbReference type="OrthoDB" id="4708870at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="464"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="509" mass="58855" checksum="7E24EB63CE60236E" modified="2023-11-08" version="1">MPGKVDYGDVDFLVSSPLHSPTSSTIDNFDWSGTVHTIKAAFETPHGRRGFLTPDCMYFAISAPNPQDDYFIQVDVKVCFNPELFAWVTFELNYASNSKMLGSMVKPLGLTIDPTGFHIRVEEMEETNFPGSMVWIANHAEDLLRILRLDRRILNAGFNTQEEIYEYFASFWLFNPAHFAARLADQKYSDRFEERSPHWTHFVKEWIPKHFPGYDAVDRTTISSEDLGKQKTELQVWYEQSRHAVRVRVFIMFPHIAKEYYTKRATYLKELDEQKLRDMISKAIPIGREGWIDDFPQPKVITKRTTPQLAPMKDSDITPPLPQGSPICKDLDSDLALPKPFLTTSQYCDPVIHPSEPWNVPIHLDPLLRTPPLTCIPHPPPANMSLEAKLNCVARWTRFDACTGLPYLLCALRDKDFEMHWTDATYAGATEEVLTTWAKEMWWHIWVRQSHVNYVGMWKKRFEKADKQAEKTRLEEEARAEAEKLVEEKREQVLCRLRALNASLGLVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL30</accession>
    <name>A0A9W9CL30_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">18S rRNA factor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ESF2</name>
        <name evidence="8" type="ORF">N0V83_007236</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the small subunit (SSU) processome assembly and function, and in the 18S rRNA synthesis. Required for the early cleavages at sites A0, A1 and A2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESF2/ABP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL30"/>
    <dbReference type="OrthoDB" id="287393at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034462">
        <property type="term" value="P:small-subunit processome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12263">
        <property type="entry name" value="RRM_ABT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039119">
        <property type="entry name" value="ABT1/Esf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034353">
        <property type="entry name" value="ABT1/ESF2_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311:SF7">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="282"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="56"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="334"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4367651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="350" mass="39549" checksum="8F8936E13AA62E34" modified="2023-11-08" version="1">MAANHIIRPKMATRKRNEYLDVEDSEDDERGYDSEEESRARMLPASKRQKTLHNSEDEEEDQDQDQESDGDEEEDAEDLADENEDIPSFSSKAAELAHLEKLAASLPSDLKLQSTKLGKSLPPPSALKKDKSGVIYLSRVPPFMKPTVLRSLLTPYGAIGRIFLTPEPSTSRTQRLRSGGTRRKLYLDGWVEFLHKRDAKFVAENLNAQTMGGKKRGRWHDEVWNIKYLSGVKWSHLVEQIQNENAERAARLRFEIQQGKKENKAFLENVERGKVQTGIEATRKRRGEDVDGGDANADATVGAISGEKEGDEKPKRRKGRLEFTQHTATSKALKKPEPGQDVHRVLSSMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLC1</accession>
    <name>A0A9W9CLC1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pre-mRNA polyadenylation factor Fip1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_006534</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLC1"/>
    <dbReference type="OrthoDB" id="1917198at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007854">
        <property type="entry name" value="Fip1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051187">
        <property type="entry name" value="Pre-mRNA_3'-end_processing_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484">
        <property type="entry name" value="FIP1-LIKE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484:SF0">
        <property type="entry name" value="PRE-MRNA 3'-END-PROCESSING FACTOR FIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05182">
        <property type="entry name" value="Fip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-mRNA polyadenylation factor Fip1" evidence="6">
        <location>
            <begin position="145"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4368178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="326" mass="35743" checksum="3B7E93AEC8FB9E8B" modified="2023-11-08" version="1">MADDSDDDLYGEATLDQQKQPKQEYDGSSGDEPMDEGQDSGDEEEDDDSESDLEIIIDKPAPVPKPAAPQPQENKAIKIEAPPQTSTTTPSSQAPKATQSTTTSGGTVPQLLPGTSYPAQRTSTIDVNGNPVYPPLGKQVLSIDMEADMAEEQKIWRRPGEDQSDYFNYGFDEFSWEMYRQRQVTMQQTLQQQKLDVANMQQMIGGPMGGMASMGSGAGQAGGGGGVPTGAPTGPGVVRVLGYGHERAGADGDDVSADGGSGYGSESDGLCVFHADGRARHGRVWWSCGTTGWWWWELSTRWWSYWWRQRWPRGKRERVVRLTHLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLM3</accession>
    <name>A0A9W9CLM3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_005963</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLM3"/>
    <dbReference type="OrthoDB" id="9983560at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF39">
        <property type="entry name" value="FAD-BINDING PCMH-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040721516" description="FAD-binding PCMH-type domain-containing protein" evidence="6">
        <location>
            <begin position="24"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="7">
        <location>
            <begin position="158"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="599" mass="65911" checksum="2EDA7D5E2A1F548F" modified="2023-11-08" version="1">MALRHWFWVVFALLSCLCPTASAQPSTSAISPARTTVPEAVAANGPLLESERIQLTDAIVDRIKNDSVASSIADLVAFEHNGTTVKRNFTNCKTFPGDVLWPSQSEWDIFNALLDDGLILTVPVTSPCYDSKWGPKDVAKCNHVVSDFTKYAIHYLGKSTGAGSVAIWTHNIKDIIFLPEYEFEGYSGKAFKVGAGVTLTEIYHAASAHGVTVLGGICPSVGYTGGYLQGGGHTPLSGLHGMGSDAVLAYQVVTADGRYVTASKTFHPDLFWALRGGGPGTYGIVTSAIVKVYPETDVTLSTYVLGNSTDGTQLVSRSDFFKALRVLWESFPTWTDANTYSFFFVFNTNGQLTLDMRNFFAVGHTPESYTALTKRFFDAVKEYGLPFQKPLNNTYYPTFLPAYMDAWGKNNFPLGLATSFPGNRLIPKSMWNNSDNLEVLWKTVVTHVEAGRHFGIYHQAPGNPQNVDNAVSSAWRNAQSFFITKSTNFAENASAEVMAEANREVNKDILQPWRDITPASEGGGSYLNEANVDEPNWKEDLYGDKYARLVEIKRKYDPWSVFYATTAIGSDEWEIRGAPIKGVTTQNGRLCRIGGHERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLM6</accession>
    <name>A0A9W9CLM6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mannosyl-oligosaccharide glucosidase</fullName>
            <ecNumber evidence="1">3.2.1.106</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glucosidase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005978</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cleaves the distal alpha 1,2-linked glucose residue from the Glc(3)Man(9)GlcNAc(2) oligosaccharide precursor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N(4)-(alpha-D-Glc-(1-&gt;2)-alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + H2O = N(4)-(alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:55988"/>
            <dbReference type="Rhea" id="RHEA-COMP:12806"/>
            <dbReference type="Rhea" id="RHEA-COMP:14355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:59082"/>
            <dbReference type="ChEBI" id="CHEBI:132537"/>
            <dbReference type="EC" id="3.2.1.106"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Glycan metabolism; N-glycan degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 63 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.106" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLM6"/>
    <dbReference type="OrthoDB" id="14419at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004573">
        <property type="term" value="F:Glc3Man9GlcNAc2 oligosaccharide glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004888">
        <property type="entry name" value="Glycoside_hydrolase_63"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412:SF10">
        <property type="entry name" value="GLYCOSYL HYDROLASE FAMILY 63 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU369107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="343" mass="39987" checksum="2619467A23314018" modified="2023-11-08" version="1">MVLIENQEYLCEEEKRLKQDRERTAYWKRWGPYVAERQWATVREDYSADGDAWSHFTHDMARSRAFRWGEDGIAGVSDSHGYQNIAFAFWNEKDDFLKERLFGLSNPQGNHGESLKEAHFHLDNTPTRKFPYEDLIKTNAKRTRLEKEYNIVDTGIFDDDRYWDIFIETAKEADDAEELLFRVTAYNRGPEPAPLHIVPHVWFRNTWAWGREEAKKKPSIAQVSDTTAQSKHHKLGDRYFQLSPSPGTGSSGDDVQPQLLFTDNDTNYKGLWNVKNKTPYVKDGIHRRIVDDEKDAVNPQNVGTKCAAWYAFDEDEGVQPGECAVVRFRFSRRNDGYLDEELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM42</accession>
    <name>A0A9W9CM42_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PAK6</name>
        <name evidence="13" type="ORF">N0V83_004984</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM42"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06609">
        <property type="entry name" value="STKc_MST3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000488">
        <property type="entry name" value="Related to severin kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000499">
        <property type="entry name" value="Serine/threonine-protein kinase KIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF10">
        <property type="entry name" value="FI20177P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="17"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="331"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="474"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="342"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="438"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="475"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="507"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="533"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="565"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="632"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4370466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="757" mass="82754" checksum="6975863DE02782E9" modified="2023-11-08" version="1">MAAELGANESVDPTTLYTKQECIGGGSFGKVYKGLDRRTGHTVAIKVIDVENAEDEVDDIMGEIMILSGMNSPYVTKYYGSYLAGSDLWIVMEFCSGGSCADLMKPGTIAEAEIAVILKELLMGLSYLHDDHKLHRDIKAANILVGANGQVKLADFGVSGQLSATMTKKNTFVGTPFWMAPEVIRQSGYDGKADIWSLGITALELANGEPPYADIHPMKVLFLIPKNPPPQLQGNFSPAFKEFVELCLRKDPRDRPNAKQLLQTNFIRKAGKPARLQELISRYQDWKARYPKQAADSEDEATPVKRNEPVNEDLWDFGTIRPVGGGRGLALAPMNDAGANARNASPQRKPVSQQRGDYGDENDDTIRASPPPSPTKRLAPLQTQGAQGDTLGTAARIPLPPSPEKKSVPFFATPAQEPPRKSPVPGFFSPPQNRGLVTPTKQQTPQQPRRQTPLAHAYDEYIQRSIAADMESMEITPQQRNPTPSRASVLPQMTIPEIPSFRRPAPSTVSESASIVQHVQPLQSPIRTQNQVPLPSQSQKPLPPLLGQQPLPPLTGQKPLPPTQQPFAPFSPSSSTRPDSASSNDPFGGPLISDWNPKPKQQPTAQASPPMPPPHSTKPSPMSRNSFGRPKPTQNHPSILQAQTPGSPTAPIEITALTGVVVPALEAAMSRRTYHLGVKNKQESAQSLGDPQGFIERKRQRQECHDRVKRLVGEIKERFEDLDRWDERGEVGMGGEVAGFLEGFLEEVLVRVEPADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMQ2</accession>
    <name>A0A9W9CMQ2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome assembly protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Notchless protein homolog 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Ribosome biogenesis factor RSA4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RSA4</name>
        <name evidence="11" type="ORF">N0V83_005214</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NLE1/RSA4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMQ2"/>
    <dbReference type="OrthoDB" id="10267436at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000092">
        <property type="entry name" value="notchless protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF0">
        <property type="entry name" value="NOTCHLESS PROTEIN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="137"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="180"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="223"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="271"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="394"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="436"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="478"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00068030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00080836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4370693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="511" mass="55607" checksum="81DF0EC53F078650" modified="2023-11-08" version="1">MATQYPPPSKRQKREQAEIARQQQDIDTTIPDGTVRVRFVDQATGESGSLPVLTVQLSQATTKNLEILLNELKDQGDDQIPYRFFADGATEALADKDDLYNALVKPGKASAESEIVLQYAPQAVFRVKAVSRCSAAISGHGDNILAVNFSPVSSSRMASGSGDKTVRIWDCDTGTPVHTMKGHSRWVLAVSYSPDGSLLASGGYDNEVRIWDPNTGKQIGGPLKGHTNFITSLSWEPYHLQEAGRPRVASSSKDGTVRVWDAIGGKIDFALSGHKGSVTCVKWGGTGRIYTASHDKTIKVWDASTGTLISTLSGHAHWVNHLALSTDFVLRTSYHDHTAKPPPTPELKLQKAKSRFEKAATLNGEIVERLATASEDCTLILWTPLSSPKPVTRMTGHQKQINHVTFSPDGALVASAAWDNHVKLWSARDGKFLNTLRAHVGPVYMACFSADSRLLASCSKDTTLKVWDMRTGKLKEDLPGHKDQVFALDWSPDGGRVGSGGADKQVRIWAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMS1</accession>
    <name>A0A9W9CMS1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMS1"/>
    <dbReference type="OrthoDB" id="3694901at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="413" mass="46246" checksum="C5AF0CAA05C3570C" modified="2023-11-08" version="1">MDTPNPFVIPFAIAQDLRDRRYWVSQLQAEMDLKSAVRAIVLRALLNNPQMDLAESRFVEIKVLLVRHEVAWLEEYVDRYPRSNIAPRLETVKQVLAAIEAAREPIDQNIMANQQPHPRQIQAQLQALGDEILEIRERLGPAMQESNRLRMEGSNLNARAIGNDDYISSLAQRLRRAEFTQQQLLDQAARTSPQMTVPVGRYDSSHGAIGRPMPPPPPAYGTSPGRSSPFLQPPPIPAPASYGSSFSRGPPPLADSGSPTTPRASSANPKPLQQKKKTPGSKDNADYLPMVIESIMANRKRFEGQGTLGVCNHTETHGNRVEKCSRPGFDVQCFQEDHIHFCRKHMRVIPGGLLACSHADKANCIVTSWEEREDWEIIVAAAFQAGNLGRKEIDKDDLMQGLFPPQLFPHRYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMT5</accession>
    <name>A0A9W9CMT5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004316</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMT5"/>
    <dbReference type="OrthoDB" id="3795350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="558"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="581" mass="65402" checksum="1556064A2A098AFC" modified="2023-11-08" version="1">MDPPRQTTTFKPTVSRVRAVGVRRLRPPVARSEPRVQGLGLAPPVRRNVPSAERPRETNRPTLMQSRTPVHPAVQSPESRKKKQKVQNDEAETMKTTFPNQSPLSVTTFPTTLPSSSAKSLAEYREDIDELLSDDVAKDFAHAVDYIIRLGGRKPKNLKARRAMLQEIVMRNKQIVSLYNAYATLHMESERRLTYRLPLFLDSELRKYGEVEVEDGDPHVTTNSVSAPVMKKLFGTLYTNLSALPERSFEETLQAIDKASMRKQGSTVQEGRENTALGGVQVDLAMDGLDSPGNATRHLTNDAGVERPEPDQPNEDATTPHDPSHHVDRTATSGSAIEQLEISHSMDVDQELPEIRQDLNFLQSSSSNHKDAQEDLKNASIIAIQNPEEFPVHRIQEVQSQYYPSTRIIAMWVHCDANGTVTQVTADVDSGSVDHLVINDSRLMSALGLLTSMQDTISLEETEFHLDETFTIKIPAGQQPPTDPQAWIVGELVSARHAYLHWLDYRRSANPRAPPYISEARTLAHELGRRAPEVWREIEKVWNLEQGDDGHRFRNDREKYLGENPSYPTEEEKAPTLNMWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNC4</accession>
    <name>A0A9W9CNC4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Origin recognition complex subunit 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ORC3</name>
        <name evidence="2" type="ORF">N0V83_004275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNC4"/>
    <dbReference type="OrthoDB" id="10265211at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20704">
        <property type="entry name" value="Orc3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020795">
        <property type="entry name" value="ORC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040855">
        <property type="entry name" value="ORC_WH_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12748">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12748:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18137">
        <property type="entry name" value="WHD_ORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Origin recognition complex subunit 3 winged helix C-terminal" evidence="1">
        <location>
            <begin position="327"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="644" mass="72613" checksum="664C26EDC98079E8" modified="2023-11-08" version="1">MWNSLRYLEGEKFDVTQSDDIIEKLFSATVASPDTHLRVGPNLCRRMLDRQKDHVQNTQDFCDGLKYAYMSHFYASYPALFLKDGIPFEDLPEDAFEAVRNLPSFRRWVEEKLEQGQAQEVRQLLNSNQYLFDQIESGIESGQQALSTLSHASQVLTSIRESLQVSPKVRLSSVWTRGASGELASSPLLRETMLCIKKIPSDRLVQLLASMEELQGDYFSVDITTFQQQINDLLENADTNAPLRTQHDVRNDSLRTTVVAQKVLLSKHKAALSEQDKAYSDLVGRFHDELEQYFAWAFIDPRTLFLSEILIYDLKSPHTEVFQPKPRLAIERALASPHDYLGCDCCSGVEGNEAALSATQPATAIVYQMYLESGTLINVSDLWSAFNAIAGDGEEESESKTMSVVCGLVHDSHMLIDIGLYSSGRWPSSNTLASSNLAGRKPTISRRSCGKGSDHLCLSTTIYYTQYQFTGFPPSPRTSRLWLRCGIAQRPTVLTRPRLLIRFKTIHRILNLRAQIRAVKRGLMHLLPTALTVPPQPIQTPRRPLLLQHHTHRILEAYGVMRCIRREQEHVSLVDVDVAELLVGGLRLVYHLEEHGAFVLVEPFCRLVDVVVGALVGAAYYHYGYVVVVYAVVVDGRFEHVGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNK8</accession>
    <name>A0A9W9CNK8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7730 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003946</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNK8"/>
    <dbReference type="OrthoDB" id="3791869at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056632">
        <property type="entry name" value="DUF7730"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38790:SF4">
        <property type="entry name" value="2EXR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38790">
        <property type="entry name" value="2EXR DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24864">
        <property type="entry name" value="DUF7730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7730" evidence="1">
        <location>
            <begin position="10"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="210" mass="24216" checksum="FD9190F74A55FBE4" modified="2023-11-08" version="1">MPIAERENALSPLLRLPAELRNQIYEYVLGGKSICLTCCVEVRKRDLKEEGFCTAEIATCVTPPGSGYDGADPEERSTLNIVKVCRQIHAETRLLIFSLNIFRYSRPVPNLDVWRQRRPECLSLITSVRLYSDARSDEAVWLDGLKYFLGLKRLEVAVHYPYVSNVPRGQQQVETKGLAARDAWEFGVVQRARQNSNWQVQVIFYHRWRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNX0</accession>
    <name>A0A9W9CNX0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N-terminal acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.254</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">NAT3</name>
        <name evidence="3" type="ORF">N0V83_002660</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.254" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNX0"/>
    <dbReference type="OrthoDB" id="5412288at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120518">
        <property type="term" value="F:protein N-terminal-methionine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019622">
        <property type="entry name" value="Rrn9_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10680">
        <property type="entry name" value="RRN9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rrn9" evidence="2">
        <location>
            <begin position="282"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="531"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="794"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="538"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="576"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="606"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="621"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="631"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="802"/>
            <end position="813"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="837" mass="93230" checksum="E3A47D61DBBABDCB" modified="2023-11-08" version="1">MSSVRVPISDAFLAALQEAFWAASLLVPASLRPQFILVGSGAMLYHGFRRRAEDLDIVGTAAAHWAFLDGAREDERFSVMTDGDLLELGDEAVPGLKYEPMAFGEVFVASLPDLWRMKARAFVVTRPGEKRADLEDYNWLVGQMVEKAVEYDPVELLEMILDELKDRLHVLSQPAGRRRDNARLRSTSTLIKMSLFGGSLPPTESPYQSDDSQHSTASSLSPPGRPTQPQTAQAPSIDSASHSGSDIEESDEDLRTRSNRFTGHPQTWNRYTAADRQIALSLEQIEQANLAAHLYNAHALKRRVRRPREDLTGIEKWQSRDRWLKTGEALQYTDLWGEIQKELVPSKDWTAWPLPPTRVPISREKATDRATEGLHDDWMIGGESTHDAGQEVREEMLAMFLRLAKETWEERASEDDSQRGRDHTTRSRSRSRSKSVISTRSRRSTSRIDVDMKDGDSIEKRNEDLQQDREEQSGPEKGKRSGRKPQDEVFAKPTLTTDDARAQRLLQPTINSMLSKLDDLALAVRRTRLNHFGRGGSSDRSSMSEFTSGVESGEFSSASSTRSTSRDAQSRKASGRPSSRATSTQTGRNAKKGKSSTANQEELLDSDVVSSDASNSDASKNKLHTRKRSRSLSSTSETGASSNRDWSGRVGLLDWSEILGLAAVRGWDERAIARTAQRCATLFGESMSFTPFEESLAVKSLEEPVHYIPSTIPAPDILPVSGPSVSKRPFYQIGTLRCPHTDCYGHIKDFALPYRVVEHCMRVHGYDPRTNDSDNEERTVGGVHIDGFLKPVTSKPGWLGHGRSKAGKASKKQKRDEEMLESFEAGMAVAEDSKPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPC2</accession>
    <name>A0A9W9CPC2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_003493</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPC2"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000117">
        <property type="entry name" value="MFS hexose transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF64">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="271"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="337"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="372"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="408"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="19"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4373200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="499" mass="54648" checksum="DA71ABDCCA6FB433" modified="2023-11-08" version="1">MEDARPWYKQPELRKLYLMMPFLFMGSTTLGYDGSLLNGLQTMPSWQAFFDYPTGSRLGLLGAFPGFGGLFVLLFTPYIADLMGRRLGTAIGCVLVILGAVLQALPSRSNPIPMYLAGRWLMGMGSNISNATCPLLITEISHPRHRGKVTTIYNTLWYVGAILAAWTTYGTLNNISSDLSWRLPTGLQCAMPGIQLLSIYLLPESPRWLISKNKHSDAKKMLTKYHGNGADNDFVNWEYNEISLTLEAENAASAESGWYELVRTPGNRKRCLLIILTAIFSQCSGNGLVSYYLAAVLKTIGITDSSEQALINGGLTIWCWLVSLGCAFLVDRVGRRILFLSAGIGMLISFTIWTACSAVYTQTGNSNAGSAVLAMIFLFYGAAGIAWPGLTVSYTVEILPFNIRAKGLTLCFVFTSLSGIFNQYVNPIGIDALGWKFYIVYVVVLVLECLAIYFLYVETKGPTLEEIAILFDGEDANVAGADMGLDHKGNVKVVKTGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPN9</accession>
    <name>A0A9W9CPN9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-(apurinic or apyrimidinic site) endonuclease 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">APN2</name>
        <name evidence="16" type="ORF">N0V83_002616</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="11">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPN9"/>
    <dbReference type="OrthoDB" id="391817at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09088">
        <property type="entry name" value="Ape2-like_AP-endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000079">
        <property type="entry name" value="DNA-(apurinic or apyrimidinic site) lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020847">
        <property type="entry name" value="AP_endonuclease_F1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020848">
        <property type="entry name" value="AP_endonuclease_F1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF4">
        <property type="entry name" value="DNA-(APURINIC OR APYRIMIDINIC SITE) ENDONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
        <property type="entry name" value="Zn_ribbon_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00726">
        <property type="entry name" value="AP_NUCLEASE_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00728">
        <property type="entry name" value="AP_NUCLEASE_F1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="15">
        <location>
            <begin position="579"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="389"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="357"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="403"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="438"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="483"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="524"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="554"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="313"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="12">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="12">
        <location>
            <position position="287"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA substrate" evidence="12">
        <location>
            <position position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4373877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="634" mass="70118" checksum="621066F2603E3DF8" modified="2023-11-08" version="1">MVRITTWNVNGIRNPFGYKPWSANRTFGAMFDILEADIVIMQELKIQRKDLTDDMVLVPGWDCYFSLPKHKKGYSGVGIYTRQSVCAPIRAEEGLLGVLCPPNSSTPYRELPESASIGGYPTPSQVADLGVDPAVLDAEGRCLVLEFPAFVLFGVYSPANSNGLRDDFRLGFLTSLDTRIRNLSKMGKKIILTGDLNVSRDLIDTAKAEDNMRSEGITHEEYLNMPNRRIFNQLVSNSKVSGQRDEGREESILYDLCREFHPTREGMYTHWEQKINARPGNFGSRIDFILCSTSIKDWFKEANIQEGLMGSDHCPVYVVTKEKVPTSGDWDAETDQAEMDQETHILDLMNPPGMFKDGARQREYHPTKDVPALSGKLLPEFTKRRNIRDMFSKKPSLPKSLPETLSLSSENDLQPQKESAGTQAFPAIATDKEPATLEETNSSTMGSATNGNTAPTLSPLKSPEKRRAPASASPTKPIKRSKSSLSGPTTSATKGQQSLKGFFQSRTKPADASPTKDNAHPYSPLSTTPTTHATGNSPPSLSTQLSIPTEPFDSDPRASQEASREGWTKLFSKKPAPRCEHGEPCITLTTKKPGLNCGRQFWICPRPIGPSGQKEVGTQWRCGTFIWSSDWKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPS0</accession>
    <name>A0A9W9CPS0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Threalose-6-phosphate phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TPS2</name>
        <name evidence="4" type="ORF">N0V83_002114</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPS0"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005946">
        <property type="term" value="C:alpha,alpha-trehalose-phosphate synthase complex (UDP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000617">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1020:FF:000003">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase subunit Tps2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000036">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase subunit Tps2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000131">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1020">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase related protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF123">
        <property type="entry name" value="TREHALOSE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4375035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="967" mass="107596" checksum="BC0B3B7E3568AFFC" modified="2023-11-08" version="1">MAPTKANSGEPESSESFEPRESEHPALHPSITNVPVTPGVHLTGKSAYFEESDAEKNNQLPFDPETATPGPSWSANSYFADQQRAESASSPSQAAAGAKTGEELLRRLSVGTDSAVKKDLADVDPRAAHPGLNLSGRIISATFAVPYSISHSPGNEWELSPRRGTSALFDSFSYLASSSSPWNHTLVGWTGEIASAKTTAAPAVQAPTNKAAAPIPVDPKQPVQVPQQPTGLRIGHEDRERLEKQLERDHGGKIVPVWLVDEVDDGKDEYILKNQSRWRTYAENELYTLFHYKQNEPADGRAARKSWADYYRMNKLFADKILEVYKPGDIIIVHDFYLLLLPSLLRQRLPNIYIGFFLHIPFPSSEFYRCLSRRKEILEGVLGANMIGFQSYSYSRHFSSCCTRILGFDSSSAGVDAYGAHVAVDVFPLGINAASTQRQAFGDPVILERINQIREMYADKKIIVGRDRLDAVRGVVQKLQAFEMFLEKYPEWQGKVLLIQVTSPSGVHTDRGDGAQEKVVNKISDLAAKINGAYGSLDFSPVRHFPQYLSREEYFALLRIADIGLITSVRDGMNTTSMEYIICQKDNHGPLILSEFSGTSGSLSGALHINPWDLGGVADAINDALKLSPQERAEQHSKLYKHVVANNVQSWTNNYLKRLMTNLSSFDQSFATPALDRAKILFQYRQAKKRLFMFDYDGTLTPIVADPQSAIPSDRVIRTLKTLAADPNNSVWIISGRDQAFLDTWMGHITELGLSAEHGSFMRHPRSEDWENLTEKTDMSWQSEVLDVFQHYTDKTQGSFIERKKIALTWHYRRADPEYGAFQARDCQKQLERTVAKKFDVEVMTGKANLEVRPRFVNKGEIAKRLILEYGDGPGEPPEFVLCLGDDFTDEDMFRSLRQSKLNPNHVFSVTVGASSKQTLASWHLVEPSDVISVISLLNGSADAGNVGAVAVVEGSIPESRAESSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQP3</accession>
    <name>A0A9W9CQP3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQP3"/>
    <dbReference type="OrthoDB" id="5372935at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038883">
        <property type="entry name" value="AN11006-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42085:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="413" mass="48197" checksum="4035CAFD4DFA91B0" modified="2023-11-08" version="1">MVRKPRTSPTQPPQTSQRGASPELGNPVKLTQYNKQTGRPIRKSAGKVRQAAGYVDSSMLEEEGFLPSTDNESEEDDDDDMDQRGRLRRLKRKRKRSPSPPSPRLEPIMYDQDMEQWTDDEQGGAFHRHTPKKQPVTLQFNVPLGFHGPLFVKLDSSLLHQNEEGRLHEMRPSKTKKVREDSPTNTQSAVQVHRAGFTSLPPELRNTIYRHLFVRKDHSKDHDLQVPPSKIGPALSRSAQFLRTCKLVHSEGCSILYGENTFHFARHFSTRGPFWEERPKEIGYQDVLHFLKMIGPENLQYLRDVKFKFDDACPRDTPYLDSNEKRRYLNDEYLINCLRILRGAKLRTFHLRFLGRRQLFKSDVKFLGYLEQIKADEVDQMGDCWHYPPKISSYVWDDLKETMTRKKKLYAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR97</accession>
    <name>A0A9W9CR97_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sphingoid long-chain base transporter RSB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_000535</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR97"/>
    <dbReference type="OrthoDB" id="4521223at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007568">
        <property type="entry name" value="RTA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465:SF8">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G14140)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04479">
        <property type="entry name" value="RTA1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4377706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="25528" checksum="CF284E2F01A997B7" modified="2023-11-08" version="1">MGAPPRPDVPEWCKSVTTDCPVAGTVYGYAPSLSANTFFASFFGLALLLQLYLGIRYKTWTYMIAVSLGCLAECIGYVGRIMMSNNPYDDLGFKIQIVLLIFAPAFLAAGIYLTLRRNRLTHNALVLWDNRRFKFFCGAVVVAYVTILIRCIYRIPELLGGWGGELMRVEVDFIILESAMIVLTVVAQTVFHPGFCFSAMANTIGKKHQHSKLASMSETELEMLGRGESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRK4</accession>
    <name>A0A9W9CRK4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nitronate monooxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_000958</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRK4"/>
    <dbReference type="OrthoDB" id="2349068at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018580">
        <property type="term" value="F:nitronate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04730">
        <property type="entry name" value="NPD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004136">
        <property type="entry name" value="NMO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32332">
        <property type="entry name" value="2-NITROPROPANE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32332:SF34">
        <property type="entry name" value="2-NITROPROPANE DIOXYGENASE FAMILY, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03060">
        <property type="entry name" value="NMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4378125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="37824" checksum="BEDE29BB85E1FDAF" modified="2023-11-08" version="1">MASSRAAPRLVDTFPWVTSPFIVGAPMRVMSGPHLALAVSKAGGLGFIGPGAKPEDTATDLTTARDLLSNLPTTTTLPPSFKSEKRLPVGVGFQLWNGDLNIAAKAVQQHSPCAAWLFAPRRGQEELDEWASRLREASPGIHIWIQIGTVQEGIEAAKSSNPPDVLVVQGAEAGGHGRATDGSGIMTLFPEVADQVRGSGISLIAAGGIADGRGIAAALALRAKGVAMGTRFLASTEARISKGYQQEVVRASDGAHSTTKTMLYNHLRGTFGWPEQYSPRGIINKSWHDQQAGVGFEKLKQRHDEAAQSGDSGWGPEGRLATYAGAAVGLVHSVEDAGVLVERLRSEATGIIRELNGQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CS65</accession>
    <name>A0A9W9CS65_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">N-acetylated-alpha-linked acidic dipeptidase 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_000643</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CS65"/>
    <dbReference type="OrthoDB" id="5841748at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08022">
        <property type="entry name" value="M28_PSMA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02121">
        <property type="entry name" value="PA_GCPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000101">
        <property type="entry name" value="N-acetylated alpha-linked acidic dipeptidase like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF71">
        <property type="entry name" value="CARBOXYPEPTIDASE TRE2, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G10650)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="4">
        <location>
            <begin position="316"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="6">
        <location>
            <begin position="506"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="5">
        <location>
            <begin position="763"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="415"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4377813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="895" mass="100333" checksum="0A159E16405E46F1" modified="2023-11-08" version="1">MSDEKHGYERAPSPPPIPTYEEATSSRNTSARLGPNEISDDAERQGLLTPDTHIQSDSRRRNGYYQAPSVQSVEDEDSQLGSPVRESQDEDLRHTMEEMEILDPGSVEEGRSRRNRSQSRFSKRFYSITHSLSSFHLPRIPWPSWRPDFTFIRQRLPTIPDEYKPGWSVIARLLGLILIVTLVYLLVVSEVVPMSGGFGQPFNPEWVRQTAMQSVESWRIEKNLEYITSYDHIGGTEGSYVLGQWIEGKFKDSHMDTYTHDEYYVYMNYPKKGGRKVAIIHPPEKAWEAKLEEPSVFSPPRAQTSAYHGLSASGNVTGPLIYVNYCDKKDFKRLWDSHVNVQGAIALCRYYGTQPDLSMKIKAAQEAGVAGVLVYSDPADDGFKKGIPWPDGGWRPGDSVQRGSVAQTNMIMGDVLTPGRPSIKKQERMSKDNNPALPKIPSLPLSWKDAQNLLRSLKGSGEQLPDDWVGGVPDVGDKWFSGHPDTSPKVNLQNGQDEVEQQRVTNVFGSLKGSEDSARKIIVGNHRDSWCFGAADPGSGTAIMLEVMRILGELRAQGWRPLRTIEFASWDAGEDNRIGSTEHVEANADELRENAIAYLNVDVGITGDKLWASGSPIFQHAWIRVLDRLTDPHENKTLKELWDTKDGKIDSPNAGSDDVAFLSIAGTSSINFGFSSDTTNSHASNPMAGSCYETLNWMAKYIDPDFRYHTLLAQIWVLLILELAQEPILPMKVEDYARALQEEGQKLLDWTESTSSGYDIEIFQPLVDSLSMFKKKADEFHKWETYWYNQVYATGGFETQAVTVQRVAHNVKIAKFESDLLDLPRDKDDHSEHGLPGRDQYKHVIFAPDANNGLEGAVFPFAKDAIEKKDWDAAAKMIKKTADVLSKAGENLHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XWX5</accession>
    <name>A0A9W8XWX5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fungal STAND N-terminal Goodbye domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_010803</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XWX5"/>
    <dbReference type="OrthoDB" id="5389929at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056125">
        <property type="entry name" value="DUF7708"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24809">
        <property type="entry name" value="DUF7708"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DUF7708" evidence="4">
        <location>
            <begin position="108"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="5">
        <location>
            <begin position="327"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="243"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4361862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="553" mass="63500" checksum="B16A98311E76AB77" modified="2023-11-08" version="1">MDDQAKTPRWYRGNHIKLLRRPQDLLKDREVWAEKVAKPDSDDQPTPDDLPANAEDIYKIRKDDLDAQLDRLRHQVDTELGNAGKSRMVLKDRRGRGMHKVSANVQGFLSDFSTFLDNYSGIVEIVKSADSQFGGLAWGTLSIFLSVAVVKQEREDEINRAILEFTRHCPRLKILKDIYPDEDLQRHIAEMYTTIIQFARSATVFYQRSSIRRIVSSSNPKDKVNETIHDIQTGLVNIRLDCEALMQQRMYQLSKQAEDLKEQLGKVNKALERSDRNRNVKQMARLRDRLQVPFGFSSTSITSYKAMLDSAIFQQRSRGGQYATFLTGNKLKEEAIFSQWLNDVHAGILFLSGKNAAWVNQTTLNWLSQAPVLLVEQFKEEENNFAYVFCQTQSILGKAQKPLIKSVFAHLALQLFEQRPKDHIDSDIEQIVSDDSWNGDNEYDALQAGTTLVEQALRTFSCDETVYLVIDRIDQCQWTHITEMMRLGVADVLNSLLRLVLTVTCKLKVLVVSASTSHNRDLRHSIARRHEPASMERFLEKVDWDQELECSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XX40</accession>
    <name>A0A9W8XX40_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_010865</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the pacC/RIM101 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XX40"/>
    <dbReference type="OrthoDB" id="6155966at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000458">
        <property type="entry name" value="pH-response transcription factor pacC/RIM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001875">
        <property type="entry name" value="pH-response transcription factor pacC/RIM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050806">
        <property type="entry name" value="pacC/RIM101"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47257">
        <property type="entry name" value="PH-RESPONSE TRANSCRIPTION FACTOR PACC/RIM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47257:SF1">
        <property type="entry name" value="PH-RESPONSE TRANSCRIPTION FACTOR PACC_RIM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="42"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="78"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="339"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="390"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="458"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="548"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="355"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="478"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="513"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="561"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4361924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="588" mass="63468" checksum="2D66E80BDC4945BD" modified="2023-11-08" version="1">MADAQSTVPATATTPQQQPLGTPTQQNAPTPASVAQSSGDQLVCQWQSCGERLPSAEQLYDHVCERHVGRKSTNNLNLTCQWGNCRTTTVKRDHITSHIRVHVPLKPHKCDFCGKAFKRPQDLKKHVKTHADDSVLLRSPEPNRGGPPQGYPGPGGKLVADLQALAATASGYQYPDSTLGNNGGYAQQHPGGAPSGFYGGPPQNSAYGPVYYAVNQSQQLNNDYEVRKRAAYDALNEFFGDAKRRNIDPNTYYDVGQRLMGLQGVQLPVLSGGYQGASMSEYGGQGGTMVAQAQHPPMHPYSLPLPNLRTKNDLLNIDQFLEQLQSTVYENPTQAAAAGVPHPGHYIHTGANYRSSNSPPGLSSSHSQSSHATAIGTNTETPALTPASSVLSYNSQHSPGAAHSASNVSPTGRTSIGSMYPTLPSVSAMSDMSAAAPSSGLAPAFDADGRRRFSSNLLQKAAPDRRGSDSMDIEDDGASPKDRRDSEQDSLHHNVHQLAIHSPKVDPALRSPSVVSSAPTETGESSQQSWVENIRVIERLRAYLKDRLEAHDYSSDDEENDAKHERRMSDDDAHNLYPVLRAVQEGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXK0</accession>
    <name>A0A9W8XXK0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_010975</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXK0"/>
    <dbReference type="OrthoDB" id="2285533at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF9">
        <property type="entry name" value="MEAB PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="5">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="94"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="267"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="193"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="267"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="298"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4362033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="362" mass="39713" checksum="E8BD248910225E8B" modified="2023-11-08" version="1">MSKIGLTKRRKIYATSEERKQRNRQAQAAFRERRTEYIKQLESTIKHHEETLQNLQNSHRNAADECLMLRYKNSLLERILLEKGIDVQAELRAKTGSPNLGPTRGPASAHASPNQAPLPQRAMLNRQQQRRSLQGPPKADGVNGLAMIQPDGPIQRSPLTQPTPGSHLSSPAQSATRSPNFVPHGQSVSPNFGPMPPQQQPLRPQPPRSNFTPMMGPQRPSLVTNQPPSNGISSASTVGSSGSGITQSSGPSSFYQTQFTDHMKQEYEEQADSFDHDDPSDQSAGPGPFPQQNFPPSNSMPPPMPPTQNPPFTSPAALAPNDGGHVFNGMNNQLFDPYDPMLDADPFGLSASMHFPTMYESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYF2</accession>
    <name>A0A9W8XYF2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYF2"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="236" mass="26807" checksum="FE5B9793AC6D299A" modified="2023-11-08" version="1">MSSSTEEHQGNYKDTEPKPHLHDFDVEFMDLHNQCLEAEASNQPPETITKIKQEAKHLKERFLLDITNLCHQNGTAWPNFTHPAFQAEDLNKASIILSQLREHDRQADPNLPAALRKANAERTGVPGCEKTNMFVTTDGSGEHQFTEYGNNWYVLQCGPLRCGELATDGSNRCSHGLKAISGNAPPGWIDRVGWKDGKNVFQLENQGLWDNPRYVWQNGVRTGPRYYGPHWDFCFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYM5</accession>
    <name>A0A9W8XYM5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Iron-sulfur cluster assembly factor IBA57 homolog, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CAF17</name>
        <name evidence="7" type="ORF">N0V83_010469</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GcvT family. CAF17/IBA57 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYM5"/>
    <dbReference type="OrthoDB" id="191995at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057460">
        <property type="entry name" value="CAF17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017703">
        <property type="entry name" value="YgfZ/GCV_T_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045179">
        <property type="entry name" value="YgfZ/GcvT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03317">
        <property type="entry name" value="ygfZ_signature"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22602">
        <property type="entry name" value="TRANSFERASE CAF17, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22602:SF0">
        <property type="entry name" value="TRANSFERASE CAF17, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25455">
        <property type="entry name" value="Beta-barrel_CAF17_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="CAF17 C-terminal" evidence="6">
        <location>
            <begin position="313"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00093447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4362376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="424" mass="47059" checksum="AC30A16FBD56A3DA" modified="2023-11-08" version="1">MPSSLLPASIRSSSYICNTCRHGLRPLQNARLVQGRAFGTASSADYNIRTRHAPLHDQRSRAVVRTPTNLYTQRFAKSSSYSTTTLTSQPSDSGIAALPHRRLVSLSGPDAAKFLQGLITNNVDPSRYRPFYSAFLDARGRVLWDVFIWTWPELSATIGKGHWACYIEVDQGEVEALKKHLKRHKLRSKIQIEDVDSEGEGGVNVWAAWGSTIETLSNEDGVTGFSDPRAPDMYRVLADTNCTMTQGIEPVATEAYHIQRFKYGIPEGPKEITRESALPMECNIDLSQGIDFKKGCYVGQELTIRTKHTGVVRKRILPIQLYNTDASNALPESGTDIKQLDESGGIKKGRAAGKFIAGIGNVGLALCRLENMTSMKVSAEGGSWKPGMEFGMETEGGVVKVKPVLHDWFVLRERDLWDKKRTRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYU9</accession>
    <name>A0A9W8XYU9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Squalene monooxygenase</fullName>
            <ecNumber evidence="16">1.14.14.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ERG1</name>
        <name evidence="20" type="ORF">N0V83_009729</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, and is considered to be a rate-limiting enzyme in steroid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>squalene + reduced [NADPH--hemoprotein reductase] + O2 = (S)-2,3-epoxysqualene + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25282"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15440"/>
            <dbReference type="ChEBI" id="CHEBI:15441"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="15">Steroid metabolism; ergosterol biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 16">Endoplasmic reticulum membrane</location>
            <topology evidence="3 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the squalene monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.17" evidence="16"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYU9"/>
    <dbReference type="OrthoDB" id="1678617at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004506">
        <property type="term" value="F:squalene monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000166">
        <property type="entry name" value="Squalene monooxygenase Erg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013698">
        <property type="entry name" value="Squalene_epoxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040125">
        <property type="entry name" value="Squalene_monox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10835">
        <property type="entry name" value="SQUALENE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10835:SF0">
        <property type="entry name" value="SQUALENE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08491">
        <property type="entry name" value="SE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 16" id="KW-0274">FAD</keyword>
    <keyword evidence="5 16" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12 16 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="402"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="458"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="488"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="18">
        <location>
            <begin position="56"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Squalene epoxidase" evidence="19">
        <location>
            <begin position="212"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF08491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ4363436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="513" mass="55905" checksum="60904A2F4DFCC7A5" modified="2023-11-08" version="1">MFSGTKITALPPGVSASSPSSQLRARGTSSSSVPKAESTYAEDYRGERRRVHHEADVVIVGAGIVGCAAAVAFGNQGRSVILLEKSLKEPDRIVGELLQPGGVNALEKLGMRDCLDDIDAIPCYGYQVSYYQEPVHIPYPENLVTSSPSKPPEGRSFHHGRFITKLRAKARATPNVTIVETTVTDVVKSDYTSQVVGVACQTGGDKDYYFGAVTLVADGYASKFRKEYLPHQPQVRSKFYGLEMIDAELPMPNHGHVILTSAPPILIYQIGTHETRILVDVPENLPSASVKAGGVKNHMLNVALPNLPACVQPAFRRAIDDGKLRSMPNSFLPPATQKTRGLIMLGDAMNMRHPLTGGGMTVAFNDVVTLSTLLSPERVPNLEDTDAVLDAMRAFHWSRKGGSSVINILAMALYSLFAAANNPYLTALKNGCFRYFQRGGACVSEPCGMLSGLIENPWTLVYHFFSVAFYAVWCRLGEEPAWKAPYVLLVESAMIVWTAASVIGPYLVWEVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ08</accession>
    <name>A0A9W8XZ08_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">glycogenin glucosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.186</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">GLG2</name>
        <name evidence="15" type="ORF">N0V83_010751</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Self-glucosylating initiator of glycogen synthesis. It catalyzes the formation of a short alpha (1,4)-glucosyl chain covalently attached via a glucose 1-O-tyrosyl linkage to internal tyrosine residues and these chains act as primers for the elongation reaction catalyzed by glycogen synthase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-tyrosyl-[glycogenin] + UDP-alpha-D-glucose = alpha-D-glucosyl-L-tyrosyl-[glycogenin] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23360"/>
            <dbReference type="Rhea" id="RHEA-COMP:14604"/>
            <dbReference type="Rhea" id="RHEA-COMP:14605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:140573"/>
            <dbReference type="EC" id="2.4.1.186"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[1,4-alpha-D-glucosyl](n)-L-tyrosyl-[glycogenin] + UDP-alpha-D-glucose = [1,4-alpha-D-glucosyl](n+1)-L-tyrosyl-[glycogenin] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56560"/>
            <dbReference type="Rhea" id="RHEA-COMP:14606"/>
            <dbReference type="Rhea" id="RHEA-COMP:14607"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:140574"/>
            <dbReference type="EC" id="2.4.1.186"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyltransferase 8 family. Glycogenin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.186" evidence="10"/>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ08"/>
    <dbReference type="OrthoDB" id="2014201at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008466">
        <property type="term" value="F:glycogenin glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02537">
        <property type="entry name" value="GT8_Glycogenin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000092">
        <property type="entry name" value="Glycogenin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="281"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="578"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="623"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="687"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="705"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4361810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="778" mass="86063" checksum="CE0E9DB3D4987329" modified="2023-11-08" version="1">MSDSYLPGAAVLAHSLRDAGTTKKLAVLVTPETLSADTITQLKSLYDYLIPVERVRNSNPANLYLMGRPDLAFAFTKIALWRQTQFRKLVYLDADVVALRALDELFNIEAPFAAAPDIGWPDAFNSGVMVITPDMGEYWALQTMAATGDSFDGADQGLLNQYFEHRPWQRLKFTYNCTPNAEYQWEPAYRHYKRDISAVHFIGKDKPWLGYRKSASGVYGELLARWWAVHDRHLREPTAAEQAKGETQSTQSGTSLSQLSAQTPAILIEPEAPATFTELSMTEPGEEAENIDQGLTEPTPTVQQRKFSAPEMQWDATRSEPPAQSQPEAANFPSQTYTFSDSHSLFKAPQAYPEPPRDMWYRVPETKPKPAEKPKPIFPWERQADRPAATRVFAEDLPVQPSPPAVSSPIHSFSTVHYEDELEATPGGVDEEVASRAHVSSPKSADVQWQAFQQSNVNAWDSVPGIDNYVRAIMDSHGRHGKTETLQQSTGTEDIGSPLLERRNRRESLILTDFPSAVERPSLPVTPAPLRRPTFWGEERNQAGELPAAEGVPDQTEWNPNEKLNELRRSSLIEFEHLKKDPHPDPPLRALPEHSVLTENDAEASHVPLSGGASPHGINHFDGASEKKNMTAEHHSLGHVRNGSATEDQLKGEQTGEEPAEQPPAPSLDKTQAPEARGARFFAERGFTSQPNSSTEHSGGHSHNSSSSTTSQQSYSTQQSQQQQSSSTSQQIQAGKTTFTLPDFGDATSGETKVGREEVLSPTQTKDNEGERTGQGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ43</accession>
    <name>A0A9W8XZ43_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alcohol dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_009839</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ43"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08297">
        <property type="entry name" value="CAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="9">
        <location>
            <begin position="16"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4363543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="356" mass="37560" checksum="D9552B6A68DA85CE" modified="2023-11-08" version="1">MDIPSTQKAAVKQGTGDDSTIAIQNVPVPQPGPDQILVKINYSGLCASDKSLLHDEWEFRMTPQASGIAGHEGVGIVVAVGSNVQDLWQVGNRAGIKWIASVCRKCEFCTNGSDECHCPKQLNSGFTIAGTFQEYALTDAHYATRLPDGVKDEEAGPIMCGGVTAYVACKRSQVKPGQWIVIPGAGGGLGHFGVQYARAMGMRVIAIDGGDAKRDLCLKLGAEKFIDFTTTSDIAAEIMKVTTYGAHGVIVFAATKAGYEQAPHLLRPAGTMVCVGLPKDPTIIAGAHPVMMCLKKLNVVGSVVGTLKDVEEALDFTARGLVHPILTHGGLEDIDRFCADMNAGKLAGRAVIKIAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZC1</accession>
    <name>A0A9W8XZC1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_009873</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZC1"/>
    <dbReference type="OrthoDB" id="410920at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000640">
        <property type="entry name" value="Serine/threonine-protein kinase PRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="339"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="159"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="290"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="666"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="479"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="381"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="525"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4363577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="686" mass="74541" checksum="A8954D68E4ADFEC2" modified="2023-11-08" version="1">MEDPESHLASSSLSTEHRLAPKADEAHVDPSPGTTLHSSAHSNATATSTLANSHLSFEPGITDPSLPRYHVRAPSTALKLETDLATFAKAPPSQALLNPSTVEGHGGEPLRQSATAPIAIQEGYLRSAWSAASLGSLSPASALSSPALNALGDITPLPSPLVMSDSPGPWQRALPRPRSRGASGSSRDDSFAMFSKGTVSPSPSLKKKGYSQLKTAALEAAAAASSQTQHKNDANHGRNRSLSEYMPESIHNARPRHVTIGNKATEADTSMQQHLHREEYLATQRGLVPARLPAGLPTPPASNASNRSVTDTEEEDVAEEDKTQYIVVRQGPQKTKKYWRPVRQLGQGTFSKVYLATCEKTTAKDPLDENVLDPRKLVAIKVCEHGPAGGADEERVELSLKREVEMLRSVSHPSLVHLQAFDHDDNQALIVLTYCPGGDLFDVASGHRDKLTVGIVQRIFAEMVSAVRYLHSQLIVHRDIKLESIAHRSLTSQICGYILLNIPVSTVPLLKSPQTHPHAIATLTDLGLSRRIPAPPESPLLTTRCGSEDYAAPEILLGQPYDGRSTDAWALGVLLYALMEGRLPFDPPPGKAASRSRAAHRIARCDWVWVSYGDEYGEWDAEKGQEWAGARECVEGLLKKVSRGRKSLEDVEKMEWVQSGIQVEGGLRKRPEDEVVQDDEARMDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZL4</accession>
    <name>A0A9W8XZL4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009723</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZL4"/>
    <dbReference type="OrthoDB" id="7777654at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001716">
        <property type="term" value="F:L-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF382">
        <property type="entry name" value="AMINE OXIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040790619" description="Amine oxidase domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="Amine oxidase" evidence="3">
        <location>
            <begin position="213"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4363430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="711" mass="80701" checksum="01CE58359022A50E" modified="2023-11-08" version="1">MPSFLRLASVVLAGVLFAQHAASEPLPRQHVSFTGPLEVEANGIQNVNVRYLSDLHGELTLAYGACDMDNIHEAHHRIGSTHIGEHPNAKRHVDWADRRPTKFVWVAPEHLQSGCLHAFIDEELVGRSDEYQVRPRKMKKRATFAEVSDPMGPWFDGVEYLKQKNANETFVAATKEKKFGIIGGGISGLMTAVSDIDFFVPLDSRANHTKLILESVGIHNWKILESSDRLGGRMHTAYLNNSKPEDYQYHELGPMRFPYSITDAETNETFAIEDQKMIFQLADVLNELNQDEDPAYQVNFIPWIQTSANAPVATSKRRPDGTIPGRMETKLDPSLLDNANSSYSNVTAVYEAMAALDDFKGLTPERIRFYAKDIFRAHKQAVELGMLDFSEVEYLRHVMGYDANITDQVTSTAVSWPMWEFETVYFMATEWRTIDKGLSRMPEAFRPLVENRTSFGTKVFGVKHNEDSNSLTVSSRPTGSDPWTVETKTEEFDYIFNSVPFNLLRFWELPPHSSLMRRAIDRTVFDGAVKIAMQYKERYWEHLEHPIIGGCGRVDIYGIGQICYPSYKINSTGPGVMLTSYISSSDAVVACAMPEAEHIAYIQRAMVELHGPIADEMFTGNYARHCWEQDEHHAGSWAVPIVPQQQLYLPAFWQTEFNTVFIGEHTSYTHSWVFSALESAVRGSVQMLLDIGLVEEAKEVNRFWMARWINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZS3</accession>
    <name>A0A9W8XZS3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009673</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZS3"/>
    <dbReference type="OrthoDB" id="265717at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053185">
        <property type="entry name" value="SET_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332:SF2">
        <property type="entry name" value="SET-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="127"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="508" mass="55728" checksum="DAB8F41E9E2B1AEB" modified="2023-11-08" version="1">MGPNHSVEIPNWVDLTEGKESEVNSDSDSSHTAVADSEQASGSVKKKKKNRKGRGKRGGKRKGKQAKQQTSEETDETDAMLTPTSTPRSDATVSTVSLSESVKKKLMDKKSAGINYVYVPVVPHYERRIVPSNSDNNTPGKSTIEVEKHGLFATEDIEHGTRIICEPALITLPTQGHGIKELMSAFAKLSKDDQEAMWKMDPSDFSASPLLTALANIADPLVDRACQLLDIPEASRTQEQSAELQDVGRVLEHALPTLRIAARWHAHCRPVTTVPQTNWSTLPGNTPVLGVFALTAQLRHSCVPNCYAHYNATSARITVHATRPIPRGDELTLSAIPSIYYQSATDRAAELLNKYALKCTCEACDPAHPKFKAHEEARMRATARAILLDNFLTVLEVVDSNKALRALHLPISYRPEREPAKDELADMILSLQELLSDLAATGAEAGVETMRWSNVLVDRLLPRFAEACRDCGEKEKGEAAWKAVVGRARLVVVLGEVLWGIVRSLLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y063</accession>
    <name>A0A9W8Y063_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009368</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y063"/>
    <dbReference type="OrthoDB" id="9991913at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12168">
        <property type="entry name" value="Mand_dh_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000526">
        <property type="entry name" value="D-mandelate dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF281">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE NAD-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="3">
        <location>
            <begin position="189"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4363915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="384" mass="42079" checksum="85280742E72CBF26" modified="2023-11-08" version="1">MSSATPTPQDSGLPPPAAPNKTSPLVASSSSSSINTSESRPTILHLGDDIRWNHELYVELGKKFKIERTYSMGREEFKQALKEKRWGDFVGMYRPFWNTGGEMGNWDKELISLLPPSCKIYASAGAGFDWVDTRTLAAHGTIYCNSASACTESVADAAIVLILSCYRAIPWSFLAARSCSPAQFQDANQNIAAVTHNPNGSVLGIVGLGRIGKRIAEKASRAFEMRVVYHDVVRMEERERELELGGEAWCASLDELLGKSDCVVLATPFAGETLLSTREFGQFKRGARLVNIARGKLVDEEALNRALDEGVVSAAGLDVHADEPRVDARIAERRNVMVLCHTAGASVESHIGFERLGMENLLGWLEKGEKGVISPVNLQWLKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y097</accession>
    <name>A0A9W8Y097_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Saccharopine dehydrogenase NADP binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009931</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y097"/>
    <dbReference type="OrthoDB" id="10268090at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051276">
        <property type="entry name" value="Saccharopine_DH-like_oxidrdct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286:SF5">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="3">
        <location>
            <begin position="11"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4363635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="421" mass="45912" checksum="9B41450F627AE513" modified="2023-11-08" version="1">MATESRQYELILLGATGYTGKLVAEWISTHLPEDLKWAIAGRNTKKLQGVVDELTKLSPNRKQPDIETCELEMEQLDNLVQRATLVITTVGPFMHYGEPVLAACVRNGTHYLDSTGEVPWIYDMVAKYDALAKKSHSIIIPECGLDSVPADVVAYVLAREVRKRYNSACERAIMTLYDFKSGISGGTALTMLELLNTFPLSHLAKSMHPYSLSPVKADNVVGTPKGSLLYRLFGLLSVPELGGVQTTGLMAGVDECIVHRSWGLYQSSSNPYGPRFRFNEYMRSRNILTAFAFKFALGLSGLFLALPPTRWLLAPLIKKFVIPSPGQGPTRESMKNDFMSYRGIGITEGGKQKVTAKLDVAHGGYAATAITLSAAAQVILRGRIGETEAGKLGGGILTPATLGDQYVETLNAFGMKISIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1E7</accession>
    <name>A0A9W8Y1E7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008922</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1E7"/>
    <dbReference type="OrthoDB" id="3946700at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="295"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="514" mass="56989" checksum="3A6D6FF8D40333CC" modified="2023-11-08" version="1">MGIPIGWNIRRDEPKEKDVLRADVTGQRSPIRRRVRPNRSPTSQGAFDPLFMVPPSRFAPQLEPVSRPSHARTRLAPPPVPEVSRTDSRSSDASRNPPSLQRDESSLRRSRNRLDGYMRAYEARAYTPVDHDRPLPALTPGFAPAAASRSAEAESLRDARDLSHIGERSPYQVIVRAGVPRLTRSRSPRSDRHAGRDTDAGEHGHETSDNNAVGFPPLRRMGRRTIADGPLPSSSLRESWSPASTLDGLGDRDRSVSPFDEHLPWDTFLSTVVPDPVGPTTESSFASAAAAASFSNSHPSSRAGSSNSAASSRTHLTVPSRRASPPPNEQFMRACDTSEDDTASDTEEEDIDVRGSIRRLNGAEAFRNRMNLSDPVPSRRRMRPSYFSNEPPLRDSERYSRRVIERSRDASAYVRSFYNPSTSRGEGDAPADRLPSLTALEAQVDGPLTAGDDVVLPPLSSFDAQPLDQELRDARSLLERLSRREDISDDFWASVGLTRSFADPVERFQERERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1K7</accession>
    <name>A0A9W8Y1K7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mitochondrial GTPase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mtg1_2</name>
        <name evidence="5" type="ORF">N0V83_009826</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1K7"/>
    <dbReference type="OrthoDB" id="269151at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01856">
        <property type="entry name" value="YlqF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1580.10:FF:000009">
        <property type="entry name" value="Mitochondrial GTPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45782">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45782:SF4">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="4">
        <location>
            <begin position="138"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="327"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="343"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4363531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="360" mass="39782" checksum="E38E3FC0C8B6B553" modified="2023-11-08" version="1">MSFIPRHAFPPLPSLPRSYFLGHHKAGLQKMKSLLSSIDLVIECRDYRIPLTSRNPLFEKALEGKERVIVYTKRDLGGGSRDNRNEDVIRMWHRPTTTMFVKNGKEAEGESSGRASVVKLLETLRAQAQARWKLVGHRVMVVGMPNVGKSTLLNALRAQGIGKGKVAATGAQPGVTRKVGSGVKIIPSEDDMESMDEGMKRKLKGVGGGVYLLDTPGVFIPYVPDAEAMMKLALCGSVKDTIIPPVMLADYLLYHLNLQSPSLYADYAPPTNDIVELLSEMARQTGRLGKGGTVDTEATALWMIQKWRSGNMGRFLLDEVDEGSLEKARLGEEGATPSLNQARKAERERRRERNKARGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1R2</accession>
    <name>A0A9W8Y1R2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">U3 small nucleolar RNA-associated protein 25</fullName>
            <shortName evidence="10">U3 snoRNA-associated protein 25</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">UTP25</name>
        <name evidence="14" type="ORF">N0V83_008590</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">DEAD-box RNA helicase-like protein required for pre-18S rRNA processing, specifically at sites A0, A1, and A2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
            <location evidence="2 10">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the UTP25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4364974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1R2"/>
    <dbReference type="OrthoDB" id="10264378at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002356">
        <property type="entry name" value="U3 small nucleolar RNA-associated protein 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010678">
        <property type="entry name" value="UTP25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053939">
        <property type="entry name" value="UTP25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053940">
        <property type="entry name" value="UTP25_NTPase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12933">
        <property type="entry name" value="ORF PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12933:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 25 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06862">
        <property type="entry name" value="Utp25_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22916">
        <property type="entry name" value="UTP25_NTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 10" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7 10" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="UTP25 NTP hydrolase-like" evidence="13">
        <location>
            <begin position="227"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="UTP25 C-terminal" evidence="12">
        <location>
            <begin position="496"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4364974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="688" mass="78003" checksum="DC8411CCD72124F2" modified="2023-11-08" version="1">MRDAPESEQSQSEASDASASEDDDDEAEPTSNPYAVLLRSLSSKPVHPHSDEPRSKKRKLRHDVANQTGLQRDGAEEVGNDSDDQVAWDENSSVEGLPESPRGVRDLARGQTEQEEALHDGSAGEQQDLEESPDNAYHTHFANPDENELAIRLKRIADNQWATSKLSAGGGRGILQTPGTEEQAPSRRKLTGTRDVGLKARLVDNAKKEIGNFDPLEQAVAPSIFGYQDLLFGARTVKNASRLRDMTCLHALNHILMTRDRVLKNNAKLAAATENDDAEYRDQGFTRPKILFLLETKQACVRVLDSITKLYDFEQQENKKRFLDSFSQPEDKFSDDKPADFRELFEGNDENEFRVGVKLTRKTLKLYSTFYNSDIIFASTLGLRRAIESGDPKKRDYDFLSSIEMIIMEQADATLMQNWEHAEYVFEHLNLQPKDAHGCDFSRVRSWYLDGHAPYMRQTIVLSAYLTPKINTLYNKHMRNVAGRLKYTPDYTSGLIESLSHGVKQTFLRFDSPSHLTDPDARFKFLNSSVLPSIMRLPRPLEGGLGVLVFIPSYLDFVRIRNSLVDSDLSYASISEYTDQTDVRKARSHFMNGKHSLLLYTGRAHHFHRYNIRGVKRVVFYGVPENPGFYEEVVGFIGKTIERGEISRAEAGVRVCFSKWERLELERIVGSKRVGKMVVDKGDVFDFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1V5</accession>
    <name>A0A9W8Y1V5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADP-specific glutamate dehydrogenase</fullName>
            <ecNumber evidence="3">1.4.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">NADP-dependent glutamate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">GDH3</name>
        <name evidence="11" type="ORF">N0V83_008662</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-glutamate + NADP(+) + H2O = 2-oxoglutarate + NH4(+) + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11612"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.4.1.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.4" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1V5"/>
    <dbReference type="OrthoDB" id="6718861at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004354">
        <property type="term" value="F:glutamate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19071">
        <property type="entry name" value="AKR_AKR1-5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05313">
        <property type="entry name" value="NAD_bind_2_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.285.10:FF:000001">
        <property type="entry name" value="Glutamate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000002">
        <property type="entry name" value="Glutamate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000030">
        <property type="entry name" value="Glutamate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.285.10">
        <property type="entry name" value="Glutamate Dehydrogenase, chain A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050724">
        <property type="entry name" value="Glu_Leu_Phe_Val_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006929">
        <property type="entry name" value="PRK09414.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43571">
        <property type="entry name" value="NADP-SPECIFIC GLUTAMATE DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43571:SF1">
        <property type="entry name" value="NADP-SPECIFIC GLUTAMATE DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6 9 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="10">
        <location>
            <begin position="427"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4365046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="689" mass="75432" checksum="63E87C0F34419B37" modified="2023-11-08" version="1">MYHNEKESGKAVLDFLSSAANTHSLTREHVHFTSKLASNSTYDAARKAIKESIKQCGLGYIDLFLLHSPYGGKKARLDSWRAVEDAIQDGEVKSGGVSNYGVKHASRPFPFLGSPGAAGVYFWKALLTTEQLQELLDSKPRIAPAVNQIEVHPFNTRKDITSFCQQHDIVVEAYAPLVRALRMKHPKIVSLSKKYSCTPGQLLVRWSLQHGYVPLPKSVKEERIVENSEIGGFEIEEADMETMDGLDEYLVTELVSTLESSSLFQKNPEYKKALEVVSVPERIIQFRVVWEDDKGECQVNKGYRVQFNSALGPYKGGLRLHPTVNLSILKFLGFEQIFKNALTGLNMGGGKGGCDFDPKGKTNNEIRRFCVAFMRELSKHIGADTDVPAGDIGVGGREIGYLFGAYRAHRNRWEGVLTGKGGSWGGSLIRPEATGFGLVYYVEHMINYASGGKESFAGKKVAISGSGNVAQYAALKVIELGGTVVSLSDSKGALIATDDKGFNPDIINQIAALKLERKSLTALENHNFKYIEGARPWKEVGKVDVVLPSATQNEVSEDEAKALIDAGAKFIAEGSNMGCTQEAIEVFEAHRKEKKGEAIWYAPGKAANAGGVAVSGLEMAQNSARLSWTSEEVDEKLKKIMKDCFENCLSTAKEYFTPAEGEFPSLVGGANVAGFRKVAAAMQDQGDWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y341</accession>
    <name>A0A9W8Y341_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Exosome nuclease subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RRP6</name>
        <name evidence="11" type="ORF">N0V83_008469</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the exosome component 10/RRP6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y341"/>
    <dbReference type="OrthoDB" id="2250022at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071044">
        <property type="term" value="P:histone mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071040">
        <property type="term" value="P:nuclear polyadenylation-dependent antisense transcript catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071036">
        <property type="term" value="P:nuclear polyadenylation-dependent snoRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071037">
        <property type="term" value="P:nuclear polyadenylation-dependent snRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06147">
        <property type="entry name" value="Rrp6p_like_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000059">
        <property type="entry name" value="Exosome complex exonuclease Rrp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.80:FF:000001">
        <property type="entry name" value="Putative exosome component 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012588">
        <property type="entry name" value="Exosome-assoc_fac_Rrp6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002121">
        <property type="entry name" value="HRDC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045092">
        <property type="entry name" value="Rrp6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049559">
        <property type="entry name" value="Rrp6p-like_exo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12124:SF47">
        <property type="entry name" value="EXOSOME COMPONENT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12124">
        <property type="entry name" value="POLYMYOSITIS/SCLERODERMA AUTOANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00570">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08066">
        <property type="entry name" value="PMC2NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50967">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0271">Exosome</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="HRDC" evidence="10">
        <location>
            <begin position="440"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="619"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="625"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="683"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="693"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="703"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="745"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4365848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="787" mass="88067" checksum="857E521B81BC2A42" modified="2023-11-08" version="1">MDSLKTLQDEISKALVSTTRSASRISGADVTFQRSLNPDVGTQLDAQNARLLHLAQRLLENAAASSDAVGPKLPDVESVDANWRGVVDVIDSLLEKADTSLDEYTGVVKRLSPAGEQAAVKARPNIAEKKIIPKPQLHFEHLPNNYETGGFRPLMTSKPHASVLLEDALKKFKDSKGREQYPHPYQAEIEAYDFPSAVYERSEPQQYQPFESTTATFVDTPEALAMMLGELKTAKEIAIDIEHHDNRSYIGIVSLMQISTRDKDWIVDTLKPWRRKLECLNEVFADPNVLKVLHGAYMDIIWLQRDLGLYVVGLFDTFHAARSLGYPGASLAYLLNRHVNFQAQKQYQIADWRVRPLSTELFEYARADTHFLLYIYDNMRNELIEKSDFSNPERNKVQDVLLKSKEVALQRYEHPVYDSHLGLGPVGWHKLLYKMPAQFTPQQFSVFRAVHEWRDNLSRREDESPLFIMPNHAVFSLARTMPADKPALYNAVQHISHLVRANADGLVKVITEAKQEGVNGPELNHVLKTIADMKEAELATKFDAPKSVAPVLVAPTPEVRPLMSTVAVPAVRALASEFWGKLSHRDVVHEQKNIPTVNVDLALPLPPLTAEIFADSNGITGATPKSEKRQHTFVAKEDRPAEDQRTDMFIVKQLGGRKRKRGDAPEVQSPSTPALDPMTNDEIMLDAQEDSADADRLREKAAKKAAKKQKKKERAAQSAGLDYDEPAFDYANAPSVLHAEDGDGKKKKKDKKKKSAGFTAFSGLTDAPKGLPRAQKEVPGRSRTFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3B4</accession>
    <name>A0A9W8Y3B4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_007978</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3B4"/>
    <dbReference type="OrthoDB" id="5401170at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4366342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="250" mass="29119" checksum="11ADA348FD93E041" modified="2023-11-08" version="1">MELQQMSSPCEARQVFTAVCVEDPLGCYRDMNEAVVKIKFQIRASQATFQYYEDNLATYQENLIHYPESRIRAQQLIDKTKAALYLCIHPTGDPNEDTVQEIVALQRSLDLQCQNAPQFLDFAESAVQPGMHREGIFRGYCVVTLMTKVPGFRVPYTTFCGWSPAQRDEMREAFKKALLEVWKCGIRPNDCALRNIVWDERERKCYIVDFEDSQLFDPERTPLPKFDEFEFRLWGIDEERAALLYKDKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3E4</accession>
    <name>A0A9W8Y3E4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3E4"/>
    <dbReference type="OrthoDB" id="3789648at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4366387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="416" mass="46287" checksum="22D783FDC883A378" modified="2023-11-08" version="1">MDSTTMSFEGKGAGAQEVHYDQVLRQITSHSLLPGVNYVNTLKVRSALTVYGNDIPELTRATEEFLDSYQDGECNCLGGQVAATMLPGEQFILSKVVELFSYSPTSPTTQSPFDLIDELIDAELLFENDTTEDEMADMAYLNVDDDAQVEADTADDNMGSNMNDYVSDTADDDFINLATTQPSELTYFDADSQQYSGYTAFNQLLHVEEDGMHLGIHRDCDDAFDFDFDVDVLEIVGQDPRDDMAESELRVTNETALGSFHFEFASSPLGMMPQPALAWSDLARRATLHSSKRKKRRPELTLTTRLEPILEEKESVSSAYSSASSGNSLDFADRREYVKPLAKRRMDFLDDEDDQDNGVESKRWSFAPNVPDIISSGHVSVVDEGIDGFFSGDIWHVEPSLALNPLAFKLPITKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3G7</accession>
    <name>A0A9W8Y3G7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">tRNA-His guanylyltransferase</fullName>
            <ecNumber evidence="2">2.7.7.79</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">THG1_2</name>
        <name evidence="2" type="ORF">N0V83_007837</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.79" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3G7"/>
    <dbReference type="OrthoDB" id="62560at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008193">
        <property type="term" value="F:tRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038469">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="309" mass="33832" checksum="46F64677B4FE50DF" modified="2023-11-08" version="1">MSTLKAARQDFEGKENAISPAQAAGTATPRKVSPPLQTNKALPSPPMNTEEEMRTREKVMGSTPQQEWSMYSAPNTQESPRSVSDADRREGRVWLDQEMSQSDLAKPASTSTPRTKSHSRLHSAPVSSATPYYPYQGFDTQALSSVDDASNSRTANTLHHTSTAEQDIQPTPPLKDSPRSKASAKAKPPTSSSSLTSSSPRESKSRDPTTTATSPQDNNANKQPKRNKGKAAAAQGGGGGWAPPPNTHQQQKQQQQPGDEGRGPVQMSRTQRDKDRKKRSRAKVLIEHVDIIRDEFWERRPWILSGKTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3Z9</accession>
    <name>A0A9W8Y3Z9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Allantoate permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">DAL5</name>
        <name evidence="7" type="ORF">N0V83_008684</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3Z9"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF1">
        <property type="entry name" value="ALLANTOATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="316"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="353"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4365067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="411" mass="45845" checksum="8D1397B04E18A72E" modified="2023-11-08" version="1">MPLICIVYFLQYIDKTSISYASVTGIIQDTNLHGNQFNWVASIFFFGQLAFEFPTIRLIQLFPLAKYVAVNVTIWGAILASLAACKSYSSLLACRFLLGAAEAAIVPAWVVFTSQWYHETNNPFASIIFLFLGLLTALIGISFWFIMPDSPEFAGFLTAEEKTLHIERIRGKEQGIGNRTFKWDQFREALTDPMTWLYAFWIFAANIPNSIATSFGNILVKGMGYTSKESLLLVTPLGAYEIVALVGGTWIAMKTEQRLYACIAVHIPAIIGAILMATTNKVPALVGYYLSGGIPIGWTTILGLRASNVAGSTKKVTVSVIGTIAYTIGNIISPQTFQARDVPRYLPAKISIVILYFLITCDLVLMRLLFVRRNRLRVDEKNARGAAWKVEENHEFLDLTDLENRESRYAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y452</accession>
    <name>A0A9W8Y452_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CENP-V/GFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_007349</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfa family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y452"/>
    <dbReference type="OrthoDB" id="6329284at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1590.10">
        <property type="entry name" value="glutathione-dependent formaldehyde- activating enzyme (gfa)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006913">
        <property type="entry name" value="CENP-V/GFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33337:SF40">
        <property type="entry name" value="CENP-V_GFA DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33337">
        <property type="entry name" value="GFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04828">
        <property type="entry name" value="GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51891">
        <property type="entry name" value="CENP_V_GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CENP-V/GFA" evidence="5">
        <location>
            <begin position="316"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4366823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="461" mass="51276" checksum="CB7FC7C18F0D111D" modified="2023-11-08" version="1">MGDGRQSNNAIKFYESRSYNYDDSWHSDFTKRFISFLDIQQGHHVLDLACGTGLLSFLEADAVGPEGQVIGIDVTPGMLAVAAHKKKLSGDKYSNLNFIRGDILHLEETEEIKEKTFDVITVASALVLLPNPKAAIEHWSAYLKPGGMIAMDATHPRNLVPGMVLERVARRLDLPIPYNRSWSKSESSLKEVLESAGVEVERIVTIDNQAGYGRRFYDIEQWDDFFVENVIVKDVARTFANNDIRRKAQGVYKEEWEKLAIDGKVEEVDSVFLGIARKRKCSEPRHARCFPAGLIKCAATDGSRYISKTVADDIFFKGGCRCGGVQYTSSAQPSDITLCHCRACQQISGSGYLPFVDVSIAGIKFPKSSTRKTLKLSNIAERTFCSDCGTPISMTYLHDPDHVSLTVSSIDFASFTSGSTPKLSKHIFLREKAPWVLLPDDGTERWGTSEFAHLLDPTRAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4P9</accession>
    <name>A0A9W8Y4P9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4P9"/>
    <dbReference type="OrthoDB" id="3565018at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35186">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35186:SF4">
        <property type="entry name" value="PRION-INHIBITION AND PROPAGATION HELO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040754058" evidence="1">
        <location>
            <begin position="24"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="298" mass="33955" checksum="E4C0E2F3C653B127" modified="2023-11-08" version="1">MSGVEVAGLVLGALPLIIQGIESYNEGLDPIKSFMRWERELPQYIRKLRNQHVHYAQTIRILFEPITAEEELAHMMTAPGSQELWKSKEMALQNKLQESYQAYQGTIGNIERITKKIASKLDLDRATELSRNDLEALIAANPKKNDKFELRKRIRFGMNKKTIKALLEELDDCNKELERFTDKSEKIETYRKTVKPSYATRLQRLQGYAKTLHDSLTVCWSCSCKSSHKTSLQLEPRHSVFATGAKKATTPSKTSFNVSFSTVSADKDVAHWLWQAAEISVEDDEEENLAPVPSPKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4U1</accession>
    <name>A0A9W8Y4U1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008291</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4U1"/>
    <dbReference type="OrthoDB" id="419598at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051609">
        <property type="entry name" value="NmrA/Isoflavone_reductase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47706:SF11">
        <property type="entry name" value="ISOFLAVONE REDUCTASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_1G12510)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47706">
        <property type="entry name" value="NMRA-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="3">
        <location>
            <begin position="9"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="318" mass="35055" checksum="DD8950B03D6F404F" modified="2023-11-08" version="1">MASGTPLPKNVLVFGATGVIGKYIIQELYNARSSFGKIGFFTSKSTAETKAGDVNGWKAKGVEVIVGDVNSEDDVNKAYKGYDVVISALGRNAIAAQIPLIKLAESAPSIKYFYPSEYGTDIEYGPASATEKPHQFKLQVRKYIRENTKKLKFTYLVTGPYSDMFFSPAGKDERIGSFNITEKKATLLGTGNEKISFTTMRDVGRLLVAALETPTTSHERTLKVNSFTTTGKEALAEFEKQTGAKWEVSYTPLDALKKVEKEAWEKDSPHKTSATLRRIWLEGGTLYNVRSNGEIGFEGKEETLEGHVREIIAKHDGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4X1</accession>
    <name>A0A9W8Y4X1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mannosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SMP3</name>
        <name evidence="13" type="ORF">N0V83_007567</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase 22 family. PIGZ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4X1"/>
    <dbReference type="OrthoDB" id="10066429at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF3">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 11 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040813593" description="Mannosyltransferase" evidence="12">
        <location>
            <begin position="18"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="208"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4367037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="517" mass="59195" checksum="95C81FFC47F90956" modified="2023-11-08" version="1">MWRRVYFLLFLVRVYFALSPSYLHPDENFQGPEVIAGRVFSYPVHETWEFTSANPIRSTFPLWLAYGWPMYILRWLWEGFGYEVSPSVVYWTLRVLMLALSVVMEDWAIHELVASPRARRVAVPLVASSYVTWTFQTHTFSNSLETLLVCWSLVLIQRIVEDKKRSGILASSLLGSMIVVGVFNRITFPAFLLLPSFSLLPHFQRKPFSLVFLSFFALFTAFIAICVDTAFYTPGEFTFSKLFSGAVITPLNNFLYNYKPANLAQHGLHPRYQHFLVNLPQLLGPAFPLLFFLRRSHISPILVSAFSGIALLSIFPHQEARFLLPAVPLILSSIRLPSPRFRKAWIWTWVIFNVVLGVLMGVYHQGGIVPVQMDIAKTNETVTHAFWWKTYSPPTWLLNGKNEQLTTIDLMGIPGEQMLEKVKEALPPCRTRKPPHAKGRGAVYLVAPRSAYFLKPYSDPSKGEDVALEEVWSYRRHLNLDDMDFADDGFINTISRVVGDRGLVVWRTTRNCWATSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4X4</accession>
    <name>A0A9W8Y4X4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007287</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4X4"/>
    <dbReference type="OrthoDB" id="408373at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046464">
        <property type="term" value="P:acylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF33">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G14860)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="162"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="444" mass="48704" checksum="2ED58510DF11E1D5" modified="2023-11-08" version="1">MPPKDHFARPIFALHIPRTPLAYPLNTMPLFDSLFSKSPPPPTSSVPSVGAVGASVGAAVGAVTQRVKLPNLGKNLFRDVQAHQLYIIIGAASLSSLILLRSVAGSTSPRVPKPIPSPREVLPPEVLRASPYPPNALEGARDIDTPYGSIRVYEWGPKEGKRVLLIHGMSTPSVALSDLAHKLVKKGCRVMVFDLFGRGYSSAPDPRVYRYDSALYISQISMALQSSSISWSPFVVVGYSLGGAIAADFASYFPNLVRGLVLVAPGGLIRTNRIGWRSKFLYSPGSILPEFFVHGLVSRRLHYKPDLASTIEPDAQIVEPGVRFNGVNLSESHGSVTEVLDWQLQHHKGFVPAFISSIRYAPIHEQHHRWGVIKDNIDKKQGRLREVWFVLGELDSVVVADEIMEDAKNVLGDENARFRLVKGVGHEVATERADEIVRVVTRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y581</accession>
    <name>A0A9W8Y581_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ASTRA-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">asa1</name>
        <name evidence="8" type="ORF">N0V83_006640</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the ASTRA complex involved in chromatin remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the ASTRA chromatin remodeling machinery complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat ASA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y581"/>
    <dbReference type="OrthoDB" id="7668193at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854:SF1">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT BETA-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="169"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="169"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="194"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="405" mass="44101" checksum="01CB442E5025E1C8" modified="2023-11-08" version="1">MCYKQSLNQHRQDNATLAGHSVPSILSDESILIAVPGRDDKKIEVYQFPDERLKFIVPRAQSTDTGMVMAVKLVHHPTSNNVLVLAGYEGGLTAVHKLPQNEGGSALELAQLVYLSKPHTQPVLSLDVSPDGTKYFTSSADAIIAAHRIPELLSYIEEKEDLSAFEQINSTQSSSSFDTGKQKVTASKAKPTESLLQVQSDQTLPSSGQDAQPSVELSESSDFSKKFVNSTASSTSISFAKQPVLSSQAQTSKPAGLSSLLSSVPAETKHKPLPPMDLTVTIEQPHKANDTKHAGQQSLTVRSDGRLLVTGGWDSRIRIYSTKTLKEVAVLKWHKEGVYAVGFGEILDAQDLSGEKEVIDDGEAIVTKRETGLGKLQRQREEQMQLKHWVVAGAKDGKVSLWEVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5S2</accession>
    <name>A0A9W8Y5S2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Anthranilate phosphoribosyltransferase</fullName>
            <ecNumber evidence="5">2.4.2.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TRP4</name>
        <name evidence="5" type="ORF">N0V83_007253</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.18" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5S2"/>
    <dbReference type="OrthoDB" id="427800at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004048">
        <property type="term" value="F:anthranilate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1030.10:FF:000008">
        <property type="entry name" value="Anthranilate phosphoribosyltransferase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.970.10">
        <property type="entry name" value="Transferase, Pyrimidine Nucleoside Phosphorylase, Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005940">
        <property type="entry name" value="Anthranilate_Pribosyl_Tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01245">
        <property type="entry name" value="trpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285:SF2">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 3 N-terminal" evidence="4">
        <location>
            <begin position="17"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 3" evidence="3">
        <location>
            <begin position="108"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4367668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="409" mass="44250" checksum="1CF48554ED3745AB" modified="2023-11-08" version="1">MATTSNEEDVKTSITPLLKRLWHESPATKPNSDEIAAALALIFTNSLSEVQTGALLTCLHFTDRDRQADVLAKCSKAMRDFATRIDVQGLEELLKKRGRKEGNYQGGLCDIVGTGGDSHNTFNISTTSSILASALLMIAKHGNKASTSRSGSADLLSCAPPKPPVITAIAPNTIYDIYSETNYAFLFAPIFHPGARHAASIRRQLGWRTIFNLLGPLANPLHDLIEARVLGVARKEIGPDFAESLRQSGCRKGMVICGDEELDELSCAGSTHCWRLVENASTGEVDINYFTVTPADFGLPEHPLSDVSPGQSPEKNAEILMKILSGEVPPDDPILHFVYINTAALFVVSGICDADSSDMGHGDDGDVITEVGPGGGRWKEGVRRAKWAITSGEAYKQWQQFVEVTNRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6I2</accession>
    <name>A0A9W8Y6I2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006735</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6I2"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4368378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="189" mass="22012" checksum="2BDFD464C19CD051" modified="2023-11-08" version="1">MYLLSQQQHSKPRRVLCSTSTTHQDHIRPVPFPPFLTHHGLRNLDRLRLLLHPKHIAKYRFLTILPQPPSPILTPQQERHILDAYQSQILTAYKALDDEVAKLMRKVVELQVHISQHRDSDPKIEEVEDKLLKKQGKLDETRGSRAAKEQQIEAEKKRLREVGTMEAATQLRELGERLGEDRKTIVLLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6L2</accession>
    <name>A0A9W8Y6L2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006049</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6L2"/>
    <dbReference type="OrthoDB" id="10249920at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047631">
        <property type="term" value="F:ADP-ribose diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006753">
        <property type="term" value="P:nucleoside phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019693">
        <property type="term" value="P:ribose phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18888">
        <property type="entry name" value="NUDIX_ADPRase_Nudt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000016">
        <property type="entry name" value="ADP-sugar pyrophosphatase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839:SF26">
        <property type="entry name" value="ADP-RIBOSE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839">
        <property type="entry name" value="UDP/ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="94"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="244" mass="27259" checksum="8117DE948E6348B5" modified="2023-11-08" version="1">MLLSLTKRPINPTKFSDRLLARLRYPASFHAFHTYPRLNAAYNMPQSPKIVKVEGLPADEAKWVEFQKISWQDQTGKNRVWEAAARKTRGKAGVDAVAISTIIRHPSRPPSTIIILQYRPPVDAVCVELPAGLVDEGESPSDASVRELHEETGYKGKLQFISPTIVSDPGLSTANMQLATVEVNLKEGDADPEQALDDGEFIERVVVPLDELYDRLVQYDKEGKTVDARLWHWAAGYHAAKTML</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6T6</accession>
    <name>A0A9W8Y6T6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007656</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6T6"/>
    <dbReference type="OrthoDB" id="3798738at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040910795" evidence="1">
        <location>
            <begin position="19"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="137" mass="14639" checksum="33EEAFECE8E70158" modified="2023-11-08" version="1">MQFTTLTLLSLAATGFSAVLPRSQYGAWDVKYSKSAYANGYQSLTLTANFTSESYPAGILSTCTTVRNPAVSLNETSSCDNDNFSYELNNEAISLTQSIELPTPQTVFGNGTLVYTISSSGKTYTAETVVDVTEAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y759</accession>
    <name>A0A9W8Y759_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GrpE protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mge1</name>
        <name evidence="7" type="ORF">N0V83_006074</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the GrpE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y759"/>
    <dbReference type="OrthoDB" id="201635at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00446">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.22.10:FF:000002">
        <property type="entry name" value="GrpE protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.22.10">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01151">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000740">
        <property type="entry name" value="GrpE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013805">
        <property type="entry name" value="GrpE_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009012">
        <property type="entry name" value="GrpE_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237">
        <property type="entry name" value="GRPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237:SF23">
        <property type="entry name" value="GRPE PROTEIN HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01025">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00773">
        <property type="entry name" value="GRPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58014">
        <property type="entry name" value="Coiled-coil domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51064">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01071">
        <property type="entry name" value="GRPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4368992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="227" mass="25673" checksum="950EDF7EC5EEA512" modified="2023-11-08" version="1">MLQRTILRASRQAARRTPAFAPLRQQPLVTRTAATPAIRWYSDAPAPKEGEAAEKKEDASSDEATKLKEQVEKKDKEIVELKDKYLRSVADFRNLQERTKREIQGAKDFAIQRFARDLVESVDNLDRAIGTVPADKLKGDENQDLVALHDGIRMTENILMNTLKKHGLERFDPSMEGERFDPNIHEAVFQAPQPDKEDGSCFHTQQKGFKLNGRVLRPAKVGVVKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7L6</accession>
    <name>A0A9W8Y7L6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutamyl-tRNA synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006824</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7L6"/>
    <dbReference type="OrthoDB" id="417697at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025255">
        <property type="entry name" value="DUF4202"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41729">
        <property type="entry name" value="GLUTAMYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41729:SF1">
        <property type="entry name" value="GLUTAMYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13875">
        <property type="entry name" value="DUF4202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4368467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="22294" checksum="C45230D42805ADE4" modified="2023-11-08" version="1">MTDTPFSKALAAIDAAHALDPNKITVNNTEAPYELHYAQKCTSYLDQRSPSASEPLRLAIRAQHFRRWEVPRSSYPMTRVGYHAWRTFLKKRQASLVEAICLEAGYPQEAAERVGALIRKEDLKEDEETQVLEDVACLVFLDDQFEAFEKEHDEEKIIKILQKTWGKMTERGHELALQIPMGERPLELVKKALAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7T8</accession>
    <name>A0A9W8Y7T8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_005799</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4370035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7T8"/>
    <dbReference type="OrthoDB" id="3789140at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="416"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="689"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="190"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="536"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="170"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="699"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="752"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="766"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4370035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="809" mass="90796" checksum="1962F39074747653" modified="2023-11-08" version="1">MLHSPAATFAHQVVDSARDRIDFVAEKFEESVGNSCSWILEYSFYRVISVAITVALPALFVLGVRQFPNTWASRHQIAVANVLHSIALANFLNYRFEDPDLLVAAIVMVAFDVFQRALGREATDAISGGESEPRPQAPSAVETLVAQTHSVLAKSDFAHDPKKGLGATLASRSGVSDTEPTPTDSKDADIVRLQRNIAELKTASQAKEVQLHSAKAELHNTRETLNATFEEYSSLREELRTVKHTLGSDHQAIIYRKDIELFALRKGNDQKETYIKERDAKLHEMRRQQKATIEIKDAQLKLLSDRLTSMDRQVHPKLKRDTKPGEGADKALEVRLLRVKKAKDSHGTGEEDKDVVIAKLREDLAVVTKAAEAVVNQQAELQRAWDIAKKVQGALKEEKEGHAQTREQLQEATVKLSEKESQSYNTPNFPSRLPTIDEDEHDTKELEAMFDTAQEDNLRLYAEVEALEKRLREANARMFTAEQSVETLKEQVRLEKAINDDLESARPSVVHHVHFQRMEGQLKENQDAIVAKDEEIKVLKNTIAEQDHHIKDLQGEARAALSSHIQNQNEIERLKHTVSELQATKEQLMLDHERLASQRTRQRVATVDRTSARSSGATLIQDLSPQLTRRSDEPAPIDALPPIPSLMESNSNDSIQLTPKRHLRSISTPNRWSLMSNDVPPPELRELKTARRKSIGLKDLMKRMIGKDERSSGDKNSVDPKERSHTSKGISAILRPKTAASTAVAVQYDEVQPQTPTNTSDPQGDELSKQKRDTPRYYATHDAKVDERPKTAAADEASRPVSRRSWAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7U5</accession>
    <name>A0A9W8Y7U5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_005409</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7U5"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF47">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="307"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="416"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="450"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="499"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="524"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="557"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="591"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="184"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4369647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="635" mass="69869" checksum="2F413ADED16AD412" modified="2023-11-08" version="1">MQSVLQYRKLRTQVERQYNRHDQQQEKPPNHPVSLNDTEAQNSQPRSSSTSTASSSEESDADKRTDPEKDAAFPDTGSRSVGPSNLRHGGKDGHDAQQMRNDVNASLENGHRHTSLRDAHTLSRTITARSFGARLGAALTGIAVHDRATYLDGVGEGQAFIVGFEGDNDSMKPQNWSRTKRMCTTLMVAAIALVVGAASSIDSPAASKAAQEFRVSEVVEILATGLYLVGFGIGALFAGPFSETLGRNPVYIITLTIYMIFIMASGLAKNIQQQLVFRFFAGFFGATPLTCAGGTISDLWRPMERTFAFPVFANAGFLGPVIGPVMGGFIAQSDAVSWRWVEWTSLIMSGLVLVLILVFQSETYAPILLRWKAQHLRKITGDPRYTTDMEIHGKPFHERLSTALSRPFLLTFREPIIVLIMLYLTVIYIVLFTFLDGYNYIFGEIHGTSVGITGLCFVSIAIGLFGASLWVPLLYKWSKRDMRAIQERGGSRLPPEFRLWHAMLGAPAIPISLFWMGWTSNSSISIWSPIAAGVLFGYGVLCVFISSYQYIIDSYEIYAASALTSVTLVRYVAAGGMTVVGIPFYENMGVHWTLTILGGISALLVPVPYLLYAYGEKIRRLSKYAAFHDEGPAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7U6</accession>
    <name>A0A9W8Y7U6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006346</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4367991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7U6"/>
    <dbReference type="OrthoDB" id="192832at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02181">
        <property type="entry name" value="GH16_fungal_Lam16A_glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF24">
        <property type="entry name" value="GLYCOSIDASE C21B10.07-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26113">
        <property type="entry name" value="GH16_XgeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040721498" description="GH16 domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="2">
        <location>
            <begin position="30"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="285" mass="30143" checksum="BD082E10A69457BB" modified="2023-11-08" version="1">MPSSSIFFGVGLALSTLSSTAFATTYKLGDTYSGSSFLDGFNFFTDADPTNGFVKYVDQTTASNSQLTKMVGTDNYIGVDYSTALTVAGGVGRSSVRIESKKTYTKGLFIVDLKHMPGGICGTWPAFWSLGSGTWPQTGEIDIIEGVNLNTQNKFNLHTDTNCKTSGVNQTGTQGLSDCALDSASGTSGCDVNAAEANTYGTGFNSATGGVYAMEWTSSAIKVWFFPRGSVPASITADAPDTSTFGTPDSNFAGACDMDTRFKDHRFIFDTTFCGDWAGNVYGMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7W6</accession>
    <name>A0A9W8Y7W6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_006376</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7W6"/>
    <dbReference type="OrthoDB" id="310217at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF98">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="7"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4368021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="322" mass="36610" checksum="2FC9AD82B21F6F9B" modified="2023-11-08" version="1">MSKVSDYMVIKRLSSSKLGGNNQGVFLVKSNITNKSYIQKLVGKTAIDAGDAQREVRAMQQSIAHPNIVHLEVWNLDYQKLGYGSIIMQHCELGSLDMLIDQFNHHHKYLKDEGFLWKVLWDVSLALCFLYTGTDIVTTRTCANDRVAVPIQRGWNRIFHRDVKPGNVFLTWKHAGGQDKCPYPTAVLGDFGSSISDADIRVGRVRANTNSRGTPAFDPPESPMYDERSDVYAMGLILHCLARMQQVSVESPEFREYHPVPQTFRDRALLTYVLKKCLAVHPIGRPMPNELPFLVWNAYKGWRYCRQDDGQVLSSWVFSNNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-06-18" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8H3</accession>
    <name>A0A9W8Y8H3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cation chloride cotransporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_005103</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC12A transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2020542at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015379">
        <property type="term" value="F:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006884">
        <property type="term" value="P:cell volume homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055064">
        <property type="term" value="P:chloride ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055075">
        <property type="term" value="P:potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034486">
        <property type="term" value="P:vacuolar transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000013">
        <property type="entry name" value="Solute carrier family 12 member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018491">
        <property type="entry name" value="SLC12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004842">
        <property type="entry name" value="SLC12A_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827:SF72">
        <property type="entry name" value="GH08340P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03522">
        <property type="entry name" value="SLC12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="252"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="284"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="381"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="422"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="497"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="530"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="554"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="132"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="10">
        <location>
            <begin position="621"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="10">
        <location>
            <begin position="1320"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="717"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1020"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1060"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1071"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1084"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1112"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1123"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1175"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1257"/>
            <end position="1300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4370582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1387" mass="152005" checksum="040D0A2FC9AF3118" modified="2023-11-08" version="1">MTDANNGQQTTRRMPPRTRSNFATRTARDEDERLHRRSFVQHSPPANEETPLLSDTGQQESQDGLAYDGSQERSQGVGVLRQWLSRRRDRSVPKKPAQHDGKRHSITETSKPRPGAFPRPVGGTGKLGTFAGVFMPVTLNVLSILMFLRFGFILGQAGLVGMMGMLVAAYTINLLTTMSISAIATNGTVRGGGAYYLISRTLGPEFGGAIGIVFYLGSVFNTSLNAVGLTDCFIDNFGTHAGDMAHWLPQSYWWQFLWATVVLAVCTLICLAGSGLFARCSNGLLIILLVAIISIPLSAVIKDPFINREEHIIFTGLSLDTFRQNLLPNFTKGAVGSVGKHKESFQDLFGILFPATGGILAGASMSGDLKHPSKAIPKGTLYGLGLTFLLYALVIFAMAASIGRETFYNNTNVIQLTNISGIVILAGEFATSLFSVLMGVIGSAKLLQALSRDHLIPGLSLFGQGTLKSDEPVYAIFITYLIAQITMFADINQIASFITMTYLMTFLVTNLACFLLKIGSAPNFRPSFHFFNYQTAAVGTVACGVTMFFVDGFYASGCVALLMVIFLLIHYTTPPKPWGDVSQGLIYHQVRKYLLRLRQEHVKFWRPQILLLVNDPRRQYKLIQFCNSLKKGGLFILGHVIVTKDFGSAVPEARRQQRSWTKYIDFSRIKAFVNIAISPAVEWGARNLVLGAGLGGMRPNIVIMGFYNLPELRQNQPSIDIPSPQPSRPSSKAANHPITRKVLQTAAKRRETSNMQSTLPTDAMRHETAIEIRNYVTIIEDLVIHLQANVAIGKGFQELDVPSAKPTMKQRTLSMLGVGNLEIDDSRKTYIDLWPIQMSAEVATAGDEPNRKNVLTTNFDTYTLILQLGCILHTVPSWKRMYKLRVCVFVEYETDIEEERGRVTTLLRNLRIEAEVLVFCLANGELGMYEVIINGKDDGDFDQIARDIDYSLEDERWWQDIKRLRRPEELSASQELAQTVDLLEAVTSWPIASFQHGRRESKPKRFAALQRMLRRAKNRTSISDLRGSGAKIGMRSQRLPADLLDDSESDPVRSASSDDGRDDDADDEAAVSESEAAVSGSNFDEYDLDASTDESDSNDSPRRPISSRRAKTTPSPSSFSKSFDLRKAIWKGSPKDTPAKEKRPTSSAFTPLRPHPGATASDTAVLRRGARQKSHSIASSSSQLSSSSRAKPTFTPPIVGPQNLPKFVSDPVPRTAVASEEAPGPSIMFVDTPKPITGGTERKDPIATAPVSPAQLAATATTNSSRISTTTNSPLSSSATALPTSSTPTHATPSSPSPASGYPAQQAIPLSFNDLPCRAQHLILNELIRRQSDDTAVVFTTLPSPVEGTCESEAESVKYISDLEVLCQGLPPVLLVHSNSMTVTMNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8N0</accession>
    <name>A0A9W8Y8N0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peroxidase</fullName>
            <ecNumber evidence="5">1.11.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_005318</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the peroxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8N0"/>
    <dbReference type="OrthoDB" id="5985073at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000302">
        <property type="term" value="P:response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044831">
        <property type="entry name" value="Ccp1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31356:SF53">
        <property type="entry name" value="HEME PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31356">
        <property type="entry name" value="THYLAKOID LUMENAL 29 KDA PROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1 5" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="6">
        <location>
            <begin position="30"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4369556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="452" mass="48627" checksum="42E5BE3EEE4CBA12" modified="2023-11-08" version="1">MFELERGENLGAALNNTLSDISSDVSVKSSASDLLALALVMSVARCGDLRVPLRLGRIDATEAGPLGVPEAHTDLNATRKAFEKAGFNQEDMITLVACGHTLGSVHSVDHPEIVSGEVAAENIAQFDTTADKFDPIVVTEYLNNTTTNPLIINANDTLNSDKRVFGADGNATMIKLKDPAVYKTQCEDVFERMLDLVPAAVTLSDPLQPVDIKPYIDTHQLKGNDSIELIGRIRIRTTTTPPIDPSDLIVTLSYLDRNGRRSNASIPTTIGTFQGGSSFGYLGENFNWFEFKTTMPASSGISAFDVVLTSHSSGAVTTFDNSGTGGYAINPYILFQETNSCLEAENNFNGTLTVVAAVHKSKLQSNSAPQLKLVRRVPLPRSFIPKLETESIPLESASAETGEFAYYKMMVPLGVASWRTSFDIEIGEGAAAGKLEFQDTSELLGATCATLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y923</accession>
    <name>A0A9W8Y923_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y923"/>
    <dbReference type="OrthoDB" id="2148716at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00531">
        <property type="entry name" value="NTF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13577">
        <property type="entry name" value="SnoaL_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="8"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="159" mass="17885" checksum="E1ACCB7E0043F0BE" modified="2023-11-08" version="1">MPGINPLDYIAVKNVLARYCEALDLKDFRLLEKVFLPDVIADYPFNSDLKGVDAVSQAIQKRLGPIRTHHNLTTQTITFDPDGKEARAVTYFNAIHFGQGPHEGKMLCAYGTYLDDLVLREADDGDHEGVQGASGIWRIKGRSVLFTRRIGDEMIMKEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9C1</accession>
    <name>A0A9W8Y9C1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Serine/threonine protein kinase</fullName>
            <ecNumber evidence="8">2.7.11.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">DUN1</name>
        <name evidence="8" type="ORF">N0V83_005218</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHEK2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9C1"/>
    <dbReference type="OrthoDB" id="407410at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001380">
        <property type="entry name" value="Checkpoint kinase 2-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FHA" evidence="6">
        <location>
            <begin position="206"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="297"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="21"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="626"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="94"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="640"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="673"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4370697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="689" mass="77222" checksum="BFC8D61231A1C3E4" modified="2023-11-08" version="1">MPAQTEKSQLKRGRVCQELEETGLRAEPSMLTGQLMQPSQGEQVEPKKARTSRVSPIPTSRLKQGPLPSPVTHKESTASDDYKEGTVTPPPSARPNQTASQAKRNPNGLSSPPSDTQPFTQFSCPPNTRTYAVDDEEGQEVWGYLVPMDDHSGDVLVLQHRAACPVPTTMVGKTTGKDKVPKGEYKKQEEEYEQEKHENGIVAGGYLIGRHRECDRILNSPVVSNRHCLFFTEKKGGDMIAVLEDLSGNGTFVNDAFVGRNKRRELNDGDEIAILDEARYVFRYPLKRNTHRFRQQYTIQEQLGKGHFASVYLCIEKASGIRYAVKKFEKRTGPGEKSKVEGLQQEIAVLMGVSHPTLLCLKDTFNEEDGTYLVLELATEGELFNWIVMKQKLTEAEARKVFVQLFQGVKYLHERNIVHRDIKPENILLTDKNLSVKLADFGLAKIIGEESFTTTLCGTPSYVAPEILENSNHRRYTRAVDVWSLGVVLYICLCGFPPFSDELYSPENPYTLSQQIKMGRFDYPSPYWDSVGDPALDLIDRMLTVDVESRITIDECLEHPWTTLDTINVNDSTDGLTGAISKLDFTKRKMQRERTMLSSINDVRVSRVIPGADGQDTVKVYEKNGVTNGTKMTKSGVKQPHPDEKRHPGEFIEMGGKGDQTLYSEGDDGSRYATDQSLASLQSRGVEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9N4</accession>
    <name>A0A9W8Y9N4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_004902</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9N4"/>
    <dbReference type="OrthoDB" id="3231000at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494:SF1">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="517"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="550"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4370384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="598" mass="68835" checksum="82DEF5628D2A170F" modified="2023-11-08" version="1">MSSEDSTTRKAEPAKTATIDMRPALWSAKAIPHNIGDPYALIGDCYERGSVAEEWEIPDPKSERLPYLAHIKSLSTSWRTLRHLADWMQVGTTPLRWNELKKRPREREQRANKTNITFIEYQPTMAPTTTPIKTPIALKEVLHALEHDKLKEPPLRLFIVEDLSQQVIELLGSRFDIDPLFFREQIDDYVWHNTRDPWAQPPSLISNMKHRQWFRMRNMRLRYYNSKESFEESRLEANTWNVLRRPDNDENHWHYQDRDGAVVSIMRTRTTVWIGKDKKCGNGTVGIVLLDPTVSQGQPLWYDRTNWLPTPKMDTEVIPSIKSSVSWYEDIVQMTAAFPWFQITEGHEVNAQVLAKPTIYTICAEWLIVCDYVKARLSQIEWELEMPRLFRSKGDAIDTSLRRLHTWRRQIPVFREMVTETLNHALPAAARLTVPASHPSFAPGSPLSTFSARSMFSDASAINFENFDGFEDIVPDFRRVLAAVNVLQERVDRLTAIVTSEISIEDSRRGLEENHNMARITWLATVFIPLTFVSGLYSMNESIAELKSTYGWYFLTAIPFTLIVMAVGWVAGGGSLAPWRAKNTKQRGMIGGKDNKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAH4</accession>
    <name>A0A9W8YAH4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fungal specific transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">DAL81_2</name>
        <name evidence="5" type="ORF">N0V83_003986</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAH4"/>
    <dbReference type="OrthoDB" id="2264294at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001080">
        <property type="term" value="P:nitrogen catabolite activation of transcription from RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050797">
        <property type="entry name" value="Carb_Metab_Trans_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN DAL81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="50"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="87"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="642"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="32"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="699"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4372212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="717" mass="80498" checksum="46AF28C77E5500E1" modified="2023-11-08" version="1">MLPRPTPVPIAAARPRKISKPSPGKENVVASKPTSASGPVSNIRSRRERPCDACRRRKSRCVIHEGAVLCVLCEFHKQECTFVQSPLPRKRKVVDGDKKDSSNNTKKRSVDSEPPPLALSTPTITATAPSPPPPQPQLNSLHLPQLGETLGLQRRQHSRYIGLSSPFDSLLIGLSQFDTRNESPFDLGTLRRVNDHECFIMLPDENTQDFAGEAEALSQVEQLVHPHGPALLDLYFQVVHPSFPIIQKHLFVERYRNGDRSFSPALLAGMYILALNWWSFNPKLANYSKPDAVRLEAIAMKALTVAMERPKLSTVQAGLLLLQRPEADSWSLTTQLVAIGQELGLHLDCSSWSVPLWERGLRKRIAWALYMQDKWSSLIHGRPSHIFNANWAVKPIAEEDFNEEGDGYDTRQEETEDEQEENHQGRILFAQMIGLTQIMAEVMDTFYTQTAIHDFANAGRKSTELILARAKPVQIKLRQWHEKLPAEVKMNAPSRGKLTSTGYLHLAYFATEITLHRRIVQSLDSTNPDSYGLFMCRNAAKTRLISAMDLVNRLKPEHLQAFWYFASKVNFTLIGTFGSLLWATAPAKEEAEFYKLRLREYRWTLSVSSKRADFLDYAVSMLDASRAMLNNLAEKPSLAEQISNAGVPPPAPPRQYSTFSNMGPPQPPQDRYSPDDMDMDDGGDLQRMSSGESFEGFNDGSQYGSRPHTAANSPRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAJ3</accession>
    <name>A0A9W8YAJ3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial</fullName>
            <ecNumber evidence="11">1.14.15.45</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">2-methoxy-6-polyprenolphenol 4-hydroxylase</fullName>
            <ecNumber evidence="11">1.14.15.46</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">COQ6</name>
        <name evidence="13" type="ORF">N0V83_004296</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">FAD-dependent monooxygenase required for two non-consecutive steps during ubiquinone biosynthesis. Required for the C5-ring hydroxylation during ubiquinone biosynthesis by catalyzing the hydroxylation of 4-hydroxy-3-(all-trans-polyprenyl)benzoic acid to 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoic acid. Also acts downstream of coq4, for the C1-hydroxylation during ubiquinone biosynthesis by catalyzing the hydroxylation of 2-methoxy-6-(all-trans-polyprenyl)phenol to 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol. The electrons required for the hydroxylation reaction are funneled indirectly to coq6 from NADPH via a ferredoxin/ferredoxin reductase system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 2-methoxy-6-(all-trans-polyprenyl)phenol + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = a 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:81183"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10858"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="ChEBI" id="CHEBI:84166"/>
            <dbReference type="EC" id="1.14.15.46"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 4-hydroxy-3-(all-trans-polyprenyl)benzoate + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = a 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoate + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:81195"/>
            <dbReference type="Rhea" id="RHEA-COMP:9514"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10930"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:64694"/>
            <dbReference type="ChEBI" id="CHEBI:78396"/>
            <dbReference type="EC" id="1.14.15.45"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UbiH/COQ6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.15.45" evidence="11"/>
    <dbReference type="EC" id="1.14.15.46" evidence="11"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAJ3"/>
    <dbReference type="OrthoDB" id="683240at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120538">
        <property type="term" value="F:2-methoxy-6-polyprenolphenol 4-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106364">
        <property type="term" value="F:4-hydroxy-3-all-trans-polyprenylbenzoate oxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000021">
        <property type="entry name" value="Ubiquinone biosynthesis monooxygenase COQ6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000245">
        <property type="entry name" value="Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03193">
        <property type="entry name" value="COQ6_monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018168">
        <property type="entry name" value="Ubi_Hdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010971">
        <property type="entry name" value="UbiH/COQ6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051205">
        <property type="entry name" value="UbiH/COQ6_monooxygenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000689">
        <property type="entry name" value="UbQ_mOase_COQ6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01988">
        <property type="entry name" value="Ubi-OHases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43876">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS MONOOXYGENASE COQ6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43876:SF7">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS MONOOXYGENASE COQ6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01304">
        <property type="entry name" value="UBIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0274">FAD</keyword>
    <keyword evidence="3 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8 11 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0830">Ubiquinone</keyword>
    <keyword evidence="4 11" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="FAD-binding" evidence="12">
        <location>
            <begin position="42"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="12">
        <location>
            <begin position="339"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4372521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="496" mass="53218" checksum="F50D89D586D46680" modified="2023-11-08" version="1">MPPRISLGALRPLSSRPATATWVRISSRRRFASSSAEAPEVYDVVCVGGGPAGLSLLAGLKASPMTSGLKVALIEGQDLQKNRLQKDASLESFSNRCSSLTPASVKNLQEIGAWPHVNLPRVQPYQEMQVWDGITGARISFDWDTAKPLLSPRNPSRPTTIAYMIENVNTTSMLLSRLEELGGVDFYTSTKVDSIDLGTDTDKVDFSSWPILTLSSGKTLAARLLVGADGANSPVRTFAGIESRGFDYNRHGVVASLKIEGAGWGGPDHKIAYQRFLPTGPIAMLPLPGNMSTLVWSTTPERAAKLKSLSPNDFLATVNAGFRLSPTDLEYLHTLPSGQEDEVAWREKHTPVDSRAIPMRVTSVQPGTVASFPLRMRHADTYTGERIALVGDAAHTIHPLAGQGLNQGQGDAAALVRTISHAVQTGQDIGSTLALESYNSERYAENNAMLGVCDKLHRLYSVESGPLVGLRSLGLRAVDAMGPLKGFFMSKAAGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBB7</accession>
    <name>A0A9W8YBB7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_004116</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBB7"/>
    <dbReference type="OrthoDB" id="2420608at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018465">
        <property type="entry name" value="Scm3/HJURP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10384">
        <property type="entry name" value="Scm3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="606"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="88"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="272"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="422"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="557"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="665"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="125"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="290"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="308"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="675"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="687"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="709"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4372342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="751" mass="83505" checksum="80A4909CBD09BA08" modified="2023-11-08" version="1">MEPPAKRLRILQSVEVDEENPDYINAKQKQQRKFKGRLESIFAKYGSMHESMSDEIDMRENRVVVDRGHLRRLVRQVNRKETILLDNLGMAAGQEPEAGSEKEEEESEYSEDELAPTQRPKSNKRRFEDTTGEQHQRSTEAVAQTSPGENNQLDLRQIAAPASTTLQAGTQEIPGTPNPAANLLQLVHFPQTPAGQQAQNTFYATLARTINHAVQQAVIPLFSNIPANTLNVQLPHMNILPAPTTPAPKDDKIAPATDPKWFFPPLSVESHKEHVAQSSPMPPPTSISVLKDKPSPAKEKGTSVGKQGHQDSRTREESLRLQNEDTPSVARSPAIKQVYSKPLSRQSSPRVEIAQKPSVKRRNYNFTEEDDIYISQKRVLDKHTWSQIRKSKRKWDGWPDMTFSYHWTKHLKKQRLHLKGVPAPSLDLREPSISRGSPAETHHLPTPSSLEHDDHPTEVGEYGSGRSDQFISSSAHFDDDERELLSLAGADTDEEQLPIAEEEAFEVTEADTILPSVELTEFINEDTLQQDLLEGLPSDNALPAGNAAVLAIVKTEPLPSSPSSKRKRETLPMSFQAMPDSEDENGEDVELSDMDIDSAIVASGTFLCDVCQKTFKTANNLDRHQANHCGAHDNIRPNSGSIDLIGDDEVQAPTTPYIKPEVSTPPPAGFLLSTPAFQTPTSLPQRSGIHSSGSKSTSKLDRKTFLKQVKQSWTKKSTPNSKTIAKSRSFQSLPKKRHWAGDAESEDELAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCI8</accession>
    <name>A0A9W8YCI8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RAD53</name>
        <name evidence="13" type="ORF">N0V83_003819</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHEK2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCI8"/>
    <dbReference type="OrthoDB" id="504170at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051598">
        <property type="term" value="P:meiotic recombination checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000470">
        <property type="entry name" value="Serine/threonine-protein kinase RAD53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FHA" evidence="11">
        <location>
            <begin position="121"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="257"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="612"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="671"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="728"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="834"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="756"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="841"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4372046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1034" mass="114630" checksum="8FB1B7AAD9F4323E" modified="2023-11-08" version="1">MEADPSQQTQPATQQVLDPRRLGRNNSGLNGGDIADVLVILHPATPTAINIVKQAAEFRPEHVLFRNSLDSMSGYLTDIEEQETIIINASGDRVGHSSHEGADLALRMSSARKLRFKELGFIFGRNPNSADIVFGQDSGKRISNQHFRIYLNTDGILMIEDMSTNGTIVDDALLKCRDDRFNKIRMLGSGSIICIQNSNDAEIIKFVVRVPSRVSYLERFRDNLRNFITECSPDHAVQRITKQYGGPTMKWDGGEHYNILGQIGKGAFATVYQLATKMAGKLLAAKELEKRRFMKNGMLDKKIDNEMKIMQSLRHPNIVEFVEYYDQGDYLYIIMEYVPQGDLQGFLNQGGSMKEELARQMAQQILGALNYLHRSKITHRDIKPDNILIADLNPFTVKLSDFGLSKVVKHEETFLKTFCGTLLYCAPEVFPDYGNGTSKGTKRRRGAKTYHSYSSSVDIWSFGGVLWYALCGEPPFKGIADTTGEAMYNNIMATPLDPTPLKKAGVSTQCIDLLMRMLRTDPSQRPTDRECLNHPWLKEGAAIPADPTLQSIVEEDESEDAEQQLSQLSIGEEIPESDEDDDILSDDELMGIVANRDPKRVRADPLYPRNQLRDHESLNAEPSFQSSHLVGEGESFQIKPMPRQPRLFGEIGASALQSSGVLSAHANDALSHEEPVRATQPGRQRDSDYTVGGAEQQVPFTAPAQLDHAFSSPSLLGAESLVRDLNMASPHSPISRTQSPFMPATPKTPDVLPHSSLDQSSKDPSQISEPTPKARPSGINRQISLPLTPSYYYNPLDPSTHNLEYASRVSGFDFVSAKNEVEKGASAYEDTLRASDTSGHSSISNPAAAPSPSNAVPSVPAELDIKPPPRRLGRLLATPDSFAPTLMLNIDKSKTSWGRLANNTIVYENSRDTRIPKTAFIIFWYSSGGQSQENVEELSQQGKDWTSLDNLHVGIWTCATHGISINGKHLKQKDDRGRALFGHLHTGDIVQVYHDARGTECLKFRCEFYLGSGKIPRPASDSFSTMLGNKLSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCQ0</accession>
    <name>A0A9W8YCQ0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_003909</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCQ0"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="14"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4372136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="359" mass="38936" checksum="75CBACF3B62E49F1" modified="2023-11-08" version="1">MNGTLLFIPPPATVIVTGSNGYIASHVVFLLLSKGYKVIGTVRNQSKREHVLSTHASHPNSSHLLVVVVEDITHSRCYLDALSSLDVEVPSAILHLAAPFSYSVSSYESDLLIPAVKGISAILEAAQILGVKRVGYEVHTNSFACIYDAALGPRPGYIYTEKDWSPLTYEDGKNATNAPTAYRAAKTVAETSAWEFIEVKKRKGKGPDFDLVSLCPAMVFGPYVNAEAVPKQSAELGESVRIIWDIITAGINAAVPPTKAPVWVDVVDVAIAHIRALEIEDAGGERFLLAAGTYCNREIVDVVRELGLGEGVPVGTPGGQERDTHFGVSATKAERILHIQWTRMKESLRRLVPQLTNIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCS3</accession>
    <name>A0A9W8YCS3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_002790</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-beta-lactamase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCS3"/>
    <dbReference type="OrthoDB" id="10250730at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07730">
        <property type="entry name" value="metallo-hydrolase-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051013">
        <property type="entry name" value="MBL_superfamily_lactonases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978:SF5">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978">
        <property type="entry name" value="QUORUM-QUENCHING LACTONASE YTNP-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="5">
        <location>
            <begin position="49"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4374051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="361" mass="40461" checksum="E566F7CE87C14C97" modified="2023-11-08" version="1">MSATPALALPASDKTVKVSLINPVNFGPAQIQRFMAPPVPGLEHFKTSPSFSFLVEHPSGRKLVFDLGIRKDHQHYSKKIAEYLPTTKYDIQVSKNVIDILREEGVNSDDIEAIVWSHWHWDHIGDPSSFPSHVDLVVGQGFKNAMLPGYPANPDSPIQESDYANRALREITFDSDLRIGQFPAYDYFGDGSFYLLDSPGHAIGHLCGLARTTTGTGRDTFVLMGGDICHYAGIFRPSKHLPVPESITPHPCSHSHPDIPFCPGSAFNDLQRSRGREPTDTVYDMTFGHDIPLAKHTMSWLQELDCDEDIFVIIAHDATVRDGVEHFPKSLNDWKAKGWGRSLKWAFFRDMETYWKDQGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCY1</accession>
    <name>A0A9W8YCY1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rpc25</name>
        <name evidence="9" type="ORF">N0V83_003791</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCY1"/>
    <dbReference type="OrthoDB" id="10256606at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04330">
        <property type="entry name" value="RNAP_III_Rpc25_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000221">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013238">
        <property type="entry name" value="RNA_pol_III_Rbc25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08292">
        <property type="entry name" value="RNA_pol_Rbc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb7-like N-terminal" evidence="7">
        <location>
            <begin position="9"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase III subunit Rpc25" evidence="8">
        <location>
            <begin position="83"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4372018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="213" mass="23927" checksum="4C2B13E474FDCB1C" modified="2023-11-08" version="1">MFILTTIQDLIQIKPQDFSRPSAQAIKDVVNEKYSNKIVPGIGLCICMWDLLSATEGLIGHGTGLVNVNIEFRMSVFRPFRGEILAGRIKTSTPDGITLDLDFTSEVFIPAHCLFDNSSFSHAENVWVWNSDGTELFLDKGEPVLFRVENEEWFDQRPTIVQKDENGEVVEERGTAWRVVGSMNQAGLGPTLWWEEQEGEGEEGDVEMEGAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCZ5</accession>
    <name>A0A9W8YCZ5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_003317</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCZ5"/>
    <dbReference type="OrthoDB" id="4074350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040804278" description="Peptidase A1 domain-containing protein" evidence="4">
        <location>
            <begin position="29"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="466"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="5">
        <location>
            <begin position="71"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="516"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="611"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="645"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="516"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="645"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="705"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4373026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="714" mass="78420" checksum="CF572CE90FAE42AB" modified="2023-11-08" version="1">MIPSRGRCAFLVSICALAASTLLLPCLADDYAQQNITVTPGYKLLPGIASVPAPVRVAPDQDWMGIDGSWNTFSLLIGDPQENVRVFVSTASQQIWAVNRLACVSNVTDPSTGQVTQMNVLDSDCETSRGNLYNNTASDTWHTKGYYQLWLEKNLGLVGNGLYGFDTVGLGMAGEKGPSVQNTTIGTLVTPNFWLGHVGLHPKPTNFSAFEDPIPSYMTDLFTQKSIPSLSFGYTAGAQYHGQTVLASLTLGGYDTSRIIPNDLTFIFAPDNERDLVVGLVGLSATTASAQNVDLLKRDDVTMLIDSTVAELWLPVEICKAFEDAFGLKYDADTDLYLVNDTLHKTLVASNPTVTFTLGQKYSTDTTLQITLPYAAFDLQASPPYRGLQEKTSYFPIRRGNESSQWVLGRTFLQEAYLTVDWERQNFSVSAIDWTFGREANLTPIVSPAYAVQEYDPPKKPGLSTAAIIGIAVGGGFVFALTMCAIGWWFWRRRHKRNLEAIKAKYEAEAAAAAAAKKDATEKSDEPPTSPIHDSEEGTRVFPKAELPGDSAVHHEMGTDLKDKEPMVISEADNAERQIYEMPGDMPERQEAGGRQLSEKETMVVRERIYNGFDPNGTPDVSPMVEEPPRRLAPISPSEVTMVNSRLPSNTNVSPVTPRTPRDGAFLETSDTFFQLPPYRPPRDGRPAEDTLLSPISPLEGSTDTSRRRFSYES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD58</accession>
    <name>A0A9W8YD58_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD58"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF8">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="102"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="371" mass="40306" checksum="306844ABFF443A21" modified="2023-11-08" version="1">MASSSEVSQSKRMTRDDLDALSIMTEEFFTHNGAPLSLGDANSIITGRNAVSPKALRDALGPVPPSIEESEIQIPIPNSSWTSRALLTRPPPSSSTTPGPLIVLYHGGGFVLGQPEGVLVYARAFTLLFNATVICASYRLAPEFPIGANDAYDILTWCATNATTTLRADPSKGFIVGGNSAGANFAAVLARRSVEEGLTPRLTGQWLSYPLMAHHDATREGVEGLVEQVGKYKELWGVSWEMNRDAPLIDAEAAKTLFSCYEPDLKSPRYNPLVRGEPREFDVARFPKALVQVAGLDIMRDDGIVYAYALEDAGVEVRLLAYKGVPHAFPAFFPTLQASKQALVDIAMGFGWLMGIEIEEEMARNVMLKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDT9</accession>
    <name>A0A9W8YDT9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">CHK1</name>
        <name evidence="18" type="ORF">N0V83_002152</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000003">
        <property type="entry name" value="Histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.40:FF:000044">
        <property type="entry name" value="Putative sensor histidine kinase/response regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000285">
        <property type="entry name" value="Putative sensor histidine kinase/response regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000010">
        <property type="entry name" value="Sensor histidine kinase RcsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000579">
        <property type="entry name" value="Sensor histidine kinase/response regulator, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047:SF46">
        <property type="entry name" value="HISTIDINE KINASE_RESPONSE REGULATOR, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12550)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="82"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="16">
        <location>
            <begin position="1887"/>
            <end position="2109"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="17">
        <location>
            <begin position="2157"/>
            <end position="2280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="51"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="464"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="491"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="2309"/>
            <end position="2361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="66"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="464"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="516"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="2316"/>
            <end position="2332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="2347"/>
            <end position="2361"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="13">
        <location>
            <position position="2211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4375072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="2361" mass="263789" checksum="339FF30C4A3661EE" modified="2023-11-08" version="1">MAPTEAESSRTARFSHILARLQELQNYVWDSEIEPFHSSYGNWHFFGYQKAPRQERPSSRGRSAVISPTTSHSPDSSRPTLARSHRSSGSDASGATSHTHTTVTDLHREGRPVVCRVSHQTLRLEREFQLSKLVVNKSDPECKHFVRSIEFVRLPTKTGEEPLVASIFEAPGPNYLKDLVSFGPNWYKFKEHENNWQPQTVEPSRGVPLLTFLDFAVGATECLEILHHGHEIVHGELRGDAFHFAETGTVKMINFGSGARSFENGLTSAGWSSLSREVGIELKLAFIAPEQTGRMPAEPDSRTDIYSLGILFYTMLCGDTPFGGPSALDVMQNVVSKRIAPASSKRIDIPEALSEIIQRMTQRNIEDRYHSTSGLKFDLIRIRELLSEGDGEGLKAFKVGSKDISCFFNLPLKQIGREKERQTIIDVIERVSKHRRSNVKSLNSLSSNSSYSDQRLDLIDDLVSDSTSSRGSESRLNSVSTTNSPVFMEAARSVHQRSQDSVVQSEGSLAEEYSETRPQLQSTSRGQSNHSLEGSYRSHHSNDSSLFRTMSNTRRLRRKARCEVVAIAGTTGLGKSRLVQSIQSTARSTGYFAMAKFDPAKKAPYEPILKLMSSLFRQIFSEADVSTDFHNSLRNFLKNTGVWTILRTYLDLPEWLLNTGGAPKVPQQKNVDFTKEMYRRGSPAVHCGGVGHTAEAWLRSGGATKSSRFMSVFIDVLRLLAVQKLCIWSLEDVQNTDTESAELVHSIVQAKIPIVLIITYSDELALPKELGSLLRSATKIQLLPFTEAQTADYVAETLYRDQEYILPLVAVVQEKSRGNLFYIREILDTCYRKKCVYYSWRENNWLFDLDKVFDVFESPEYGSSVTTDFIVKRLFELPPATKKLLAWASLLGGSFSFELVKKLLSPETRPAADARLPLLDEAESAIQALNSALNAYVLMPAEQDARFRFSHDRYLMAAASSLEHDWDTQLMHFMIAKAITSSPDYHDDSMGSKALYIRSRHICLAAELIKAKETRRAPFRDVLYQAGETACESGARSTGTYYFAHSLMLLQDDPWDDKQPDVSYQETLQLFVRTAECYWHQGMLDEALSIIRETFKHARDPCDMASSFILQSRVFAVRGDSFGAFQALKDCLSLLGSPLPPTTWEECDADFQKVYRTLQDTNKDELLSRRPSTDDRVLMTMGPIFIELLSAAFWSNSLVFYQATLKLINLHLDRGTMSQVALAYVHLGSIAGGRFNMINFATDMGGIAKRIFQLFPEDHYTLGRGQTLQPLFLGHFEAPIGDLISSLEIALQASLTAGDRILTLLNLGVQAHFRIMASCDVAELEAWIDETPLDTTWHQDLRGGIFLMACRQYSRALQGKTSTGNASSVFSDPEHDEANYVEFIEKTTSNPKRPKSFYLATKLPVLVLFGYIPEAIALGELLLPMLSSLWSERFNYSVRYYLSLAYMATLRDEPKHARREAMIQHVRDTLQLLETCCTITDINYRGWVHLLTAVLGDVNVDPPSALQNYEAAMDHSEMNGFVLDEAFAHELYAEWLLRKKALRAARHSLKDCISTYRRISAFGKANHVASKYEWLLRGGASLTTMDAAVQTTVIDTGNTTLRLEQNEDQEQYLGAESAVDRTQNWIVPETAKRHESAQDLHNGFSAVGLDMLDLSSILESSQVLSSELVVDKLLAKMALMILESTGATLCGIVIEDAQVEWSIACVATNEPNNDSGFSPGVTSFPTGQPLDTVDNVVARQVTLYTLRFRETVFVQNLLEDDRFSNVSEAYLGRNPDGKAVICIPIVHSDKLLGSIYVEGPPNSFTERNTQVLRLLVAQISISLANALLFKEIERFSASNEAMLEMQKRALAQARAAEIKAKEAEAIAIRNMKLKEEAAKAKSLFLANVSHELRTPLNGVIGMSELLKASILNSEQAGYADSIRVCADTLLSIINDLLDYSKLEAGKMNVMVMPLSLNETITEVVRALAYTNAERGLKTIEQLELDPEMLVMGDPVRLHQILMNLLSNSYKFTPKGSVTVRAVVDMEADDWVDVTCSVIDTGIGIPDEQKKKLFLPFSQIESSSSRSYGGTGLGLSICKALIENVMHGKIGLESEPNSGTTVWFSLRFQKVPRAQALNGPRTRDPDPMAKFSSQENNGHEQSSGTIIDLKKVPRNELRICIAEDNLINQRIAISFVQKLGFKCDAYLDGFKTIDALERGSQNGRPFHLVLMDVQMPHCDGYEATRLIRQHSNPEIRNVLIIAMTASAIQGDREKCLESGMNNYLAKPVRAKTLKALLESYLNKEDDGEEIPDIQAEAKKLVRLALSEAEQGVVEPNAARKDLKVDEEGGEKMKSRPPSIRMNTTQRIPQHELNGEKESPPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDW0</accession>
    <name>A0A9W8YDW0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sulfate adenylyltransferase</fullName>
            <ecNumber evidence="9">2.7.7.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP-sulfurylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Sulfate adenylate transferase</fullName>
            <shortName evidence="9">SAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 13" type="primary">MET3</name>
        <name evidence="13" type="ORF">N0V83_002547</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the first intracellular reaction of sulfate assimilation, forming adenosine-5'-phosphosulfate (APS) from inorganic sulfate and ATP. Plays an important role in sulfate activation as a component of the biosynthesis pathway of sulfur-containing amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24152"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>sulfate + ATP + H(+) = adenosine 5'-phosphosulfate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18133"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="EC" id="2.7.7.4"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Allosterically inhibited by 3'-phosphoadenosine 5'-phosphosulfate (PAPS).</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8 9">Homohexamer. Dimer of trimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">The adenylyl-sulfate kinase (APS kinase) is non-functional. It is involved in allosteric regulation by PAPS. PAPS binding induces a large rotational rearrangement of domains lowering the substrate affinity of the enzyme.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the C-terminal section; belongs to the APS kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the N-terminal section; belongs to the sulfate adenylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.4" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDW0"/>
    <dbReference type="OrthoDB" id="468at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004020">
        <property type="term" value="F:adenylylsulfate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019344">
        <property type="term" value="P:cysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070814">
        <property type="term" value="P:hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010134">
        <property type="term" value="P:sulfate assimilation via adenylyl sulfate reduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019379">
        <property type="term" value="P:sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02027">
        <property type="entry name" value="APSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00517">
        <property type="entry name" value="ATPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.400.10:FF:000003">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000802">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000052">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.10">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03106">
        <property type="entry name" value="Sulf_adenylyltr_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002891">
        <property type="entry name" value="APS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059117">
        <property type="entry name" value="APS_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025980">
        <property type="entry name" value="ATP-Sase_PUA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027535">
        <property type="entry name" value="Sulf_adenylyltr_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050512">
        <property type="entry name" value="Sulf_AdTrans/APS_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024951">
        <property type="entry name" value="Sulfurylase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002650">
        <property type="entry name" value="Sulphate_adenylyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00455">
        <property type="entry name" value="apsK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004040">
        <property type="entry name" value="PRK05537.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00339">
        <property type="entry name" value="sopT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42700">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42700:SF1">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01583">
        <property type="entry name" value="APS_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01747">
        <property type="entry name" value="ATP-sulfurylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14306">
        <property type="entry name" value="PUA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 9 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-sulfurylase PUA-like" evidence="12">
        <location>
            <begin position="4"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Sulphate adenylyltransferase catalytic" evidence="11">
        <location>
            <begin position="174"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="APS kinase" evidence="10">
        <location>
            <begin position="395"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Allosteric regulation domain; adenylyl-sulfate kinase-like" evidence="9">
        <location>
            <begin position="395"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="197"/>
            <end position="200"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="291"/>
            <end position="294"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="434"/>
            <end position="437"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="515"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="9">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="9">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="site" description="Induces change in substrate recognition on ATP binding" evidence="9">
        <location>
            <position position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4373808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="573" mass="63980" checksum="CDAFEA6B989A2256" modified="2023-11-08" version="1">MANPPHGGILKDLIARDAPRRKELFDEAETLPAIVLSERQLCDLELILNGGFSPLEGFLNQKDYDGVVANNRLADGNLFSIPITLDVSKETIDEVGVKAGARIALRDFRDDRNLAIITVEDVYQPDKQKEAKEVFGGDAEHPAIKYLFNTAKEFYVGGKVDAIDRLEHYDYVGLRYTPAELRLHFDKLGWSKVVAFQTRNPMHRAHRELTVRAARARQANVLIHPVVGLTKPGDIDHFTRVRVYQALMPRYPNGMAVLALLPLAMRMAGPREAIWHAIIRKNHGATHFIVGRDHAGPGKNSKGVDFYGPYDAQDAVEKYRDELGIEVVPFLQMTYLPDSDEYKPKNEVEQGIKTLDISGTELRKRLRTGQEIPEWFSYPEVVRVLRESHPPRSQQGFTVFLTGYQNSGKDAIARALNVTLNQQGGRSVSLLLGDTVRSELSSELGFSRSDRDKNIARIAFVASELTKAGAAAIVAPIAPFEESRQHAREAVERYGSFYLVHVATPLEHAEKTDKRGIYKKARAGEIKGFTGVDDPYETPKKADLTVDLSTSNVRTAVHQIVLLLESQGLLTQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEG8</accession>
    <name>A0A9W8YEG8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002268</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEG8"/>
    <dbReference type="OrthoDB" id="2530523at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="350" mass="37684" checksum="5020C06A534B1034" modified="2023-11-08" version="1">MDYQTFNNHNPSFGAQFSASTAPQHSQFYTEPQARLQQSNPSFPYGQFPNGQGASFPGMGGAMGGSAMQPGAMPASLNQARAALQQQQQQQQLSSPSPYSAAPYSQTLPSPAHQQFAQNRQTASPASASGHPYATPQPQQSPSTLPSNGQQQPMNPQAPVKSEPAQAQTPVKAVPPSPMSPVAHARNQDRMATLLEINSILIKEVCDLQTQGKAGQVGAAPDGKTEGDKPQPSKEYVDYMRRLQANLAYLAQNAEKNPKPGQQIQPGPAIMSAPASPVELVKLYQKLQELFPGWKGQMAQMKQSPGPQRLNSNTSQPPNSAGLQQNWSQNAMQNMQQAQQQAQQPKPEQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF02</accession>
    <name>A0A9W8YF02_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001493</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF02"/>
    <dbReference type="OrthoDB" id="3673077at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="644" mass="69948" checksum="AC16593C6EE66801" modified="2023-11-08" version="1">MEGQKPELGPRNLSAFNPEAANFRPTQAAGITPVTESVQSTTAAPSTMPQGHLRMPMAPTHGAPVTSASNAGSAYERGAAMAHVPSGLGDPRARDVPFDDLQRMLQEHHIDHEHGTTGVPLRGYAMNRPATRHPPSRFQPSFQTNRIPASLDELSASFRLRNVENDLMIDESGPYEGGRDPLFAQYVDTSRRGSSDRSAASRGHQQPLMGLGAPGVMAADTTRVVQPPPGFLGLDARVPAEDHMSVPSTVMEHGYVPSGPSVITEPRHVPTGPAAFRSPPRLRPRRPSRPMRPKRTDQGPEPSAADIYPEDAIWDPSQQPYVGKGKEPMRDPVYQHPLLFVPESSPQQELVVTDTASWPTPAEMYAGPGPAAVRAQNPDPRPQALGDVVYGQSQQSYVGKGKEPMKEPMHSPPSSVPHSSPEQELLVTDTASWPTPAEMYLGSAPAAAHPSRSNMPLPAPGDVVWGLSQEPYVGKGKEPLKEPMHSPPTLVPHSSPQEEELVVTDTASWPTPAEVYLGPGRGPAAVRPRAPYPPPREPEFNIFENHVAPTAEDMNDADAEVEALMNQIPHLTVNRLLTYGAPILLCDPRPLTPGQLDGSRYSLRIGRSWDPIYPGEGTPFRIRPRNHEGWGGWEWALKNGWGQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFP4</accession>
    <name>A0A9W8YFP4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SRR1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFP4"/>
    <dbReference type="OrthoDB" id="5318346at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012942">
        <property type="entry name" value="SRR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42080">
        <property type="entry name" value="SRR1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42080:SF1">
        <property type="entry name" value="SRR1-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07985">
        <property type="entry name" value="SRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRR1-like" evidence="1">
        <location>
            <begin position="90"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="286" mass="32455" checksum="3DF29B691A04F990" modified="2023-11-08" version="1">MGGQGRTRGRGRVKRKEVETEDGWTVVTHGLSNLSIGKDGGKSGCGSEKVAGALPTDVVQGLTAEKLMGDFKKLQERWEDTSMAKQIEEILNMRHCNIDSAVCIGIGSLSRDWAHRWRSLWQLVLFVDVVTRCKSKTRREAGVKTYAQDPAFTPLDVTFLKELDIEVTETGIENHMVETTFVYSPFVDWYLLLPLFLKGKDPLLYVGNEVLDDYTPYGQSQEKKAKLEECNKLGKDFLVVRDRVKLKEFDLHANALNGMVAYWRREEDLEEMKEDMRAKNGEEEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFR5</accession>
    <name>A0A9W8YFR5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001471</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFR5"/>
    <dbReference type="OrthoDB" id="3945172at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="122" mass="13004" checksum="0F6CF2F3C3195F03" modified="2023-11-08" version="1">MYRNLASASIRAAAPARFIRPFTVSASRPVKQYPNDLSTDDSVKTDAYPDDKHATEKAKEGDTHNVQESNAKAGMDSAHKEGTGGHATEGRDAAGGKETQKKEFPEAPDSIGMQDERGGRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFX2</accession>
    <name>A0A9W8YFX2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001782</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFX2"/>
    <dbReference type="OrthoDB" id="5304354at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="5"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4376498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="362" mass="40755" checksum="0D0FAACEABFD35FE" modified="2023-11-08" version="1">MSIATMSLNELPPELKLAIAEILDPQSCLHLTLTCRDLYNLCQTMLEEHERLFSELRIIRSDQTNRHVLWTTLRAVLKDPRKGWYVRELDLPASRGYHWDRDNLGQHGNQPPNPLGDLPEKDQELFQQAARALERLYCVADEDFPDDGVDIMELIRTIELRIGAGYDDGIIAVLIHHLPFLQTIRITDLETIALEDMLVKIAAAYKNQSKVKGLPLQRLTSASVAHWDSEYSCSSSWAADLLSLPSMHFFAANAMSGHHDFYGNPDTAFAGIMPYSNLTELYLISCAFDVENLEAILAGIRGLRKFTYTDGGATVSDSEFEPKKIIAAVEKRAGHSLEELRLDRDMADASVCHPILLEFRGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG49</accession>
    <name>A0A9W8YG49_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG49"/>
    <dbReference type="OrthoDB" id="3795090at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="405" mass="46827" checksum="E452295E2B62B427" modified="2023-11-08" version="1">MQFCDKSPPAPKSSAYVTEIMDTISTNLDRDDLPQAAEVCREWRDRVPLWYETSTPCVEELDPHFDEPCEAYKEQEKEFDKLRTSLSAKHDIPFPREVTRDLSIIVRRDDFTYDGLPVLRAMRGVDAEVFQNVEDEQRTGFEEDPPEYRTLCVRQGFQRVRIPNGWPVGLREIYCIICDGFHTRFRFENLHPMLRFLESLTVCFRGHGSILRLTIGTIAEYRAPKSCYEHYREEMLKFAHYLRYATNLATADTFGNHLATRPLITKLVTTARPKPNGEPNEDCEYYSVIQDTEGLKLKVVVLFLLKAFRSHLAMRIEKEFKDMGGKINREPHLLAAFPGDEWETYKTNFEKEIPGMYREGVEAVNEIMNGTKGWEPIVWDNLGDEPLREVAEPSIPQSPKQSGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG63</accession>
    <name>A0A9W8YG63_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001741</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG63"/>
    <dbReference type="OrthoDB" id="332863at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070290">
        <property type="term" value="F:N-acylphosphatidylethanolamine-specific phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070291">
        <property type="term" value="P:N-acylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070292">
        <property type="term" value="P:N-acylphosphatidylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000048">
        <property type="entry name" value="Zn-dependent hydrolase/oxidoreductase family protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024884">
        <property type="entry name" value="NAPE-PLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15032">
        <property type="entry name" value="N-ACYL-PHOSPHATIDYLETHANOLAMINE-HYDROLYZING PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15032:SF4">
        <property type="entry name" value="N-ACYL-PHOSPHATIDYLETHANOLAMINE-HYDROLYZING PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038896">
        <property type="entry name" value="NAPE-PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="3">
        <location>
            <begin position="55"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>an N-acyl-1,2-diacyl-sn-glycero-3-phosphoethanolamine</name>
            <dbReference type="ChEBI" id="CHEBI:62537"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>an N-acyl-1,2-diacyl-sn-glycero-3-phosphoethanolamine</name>
            <dbReference type="ChEBI" id="CHEBI:62537"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR038896-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4376457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="387" mass="42776" checksum="0D71BBF4DEAEF85C" modified="2023-11-08" version="1">MPWRKITGQTNQPDTTPPTVTVQKPTFLPTRSTPKLRSTWLGHACYYIEYPSGLRVLFDPVFEDCCAPIQLKGLKRYTPPPCEIEDLPFVDVVVISHNHYDHLSYPTTQRIQKKFPEAHFFAPLGNKKWFESSGVPSDKCTELDWWESRDITLSVIKEAEKEGRVASMSVSSSFAEEEGGEKNSRSNITATITALPAQHLSARGPFDKCKTLWASWAVRSTPSSTTSPAPPAAASNVYFAGDTGYRSVPASIATAPDVDDYAPEHLHLPVCPAFSQIGTHHGPFDLGLIPIGAYAPREFFSNVHASPKDAVNILVDTKCRRALGMHWGTWVLTEEEVMEPPVKLREALRWKGVEEVGVFDVCDIGESREFDSGGIEEEGGGGLEVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG87</accession>
    <name>A0A9W8YG87_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">[4Fe-4S] proteins maturation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ISA2</name>
        <name evidence="4" type="ORF">N0V83_000177</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HesB/IscA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG87"/>
    <dbReference type="OrthoDB" id="1938621at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="ATAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="ATAP_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00049">
        <property type="entry name" value="iron-sulfur cluster assembly accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43011">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43011:SF1">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core" evidence="3">
        <location>
            <begin position="147"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="103"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4377352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="262" mass="28189" checksum="3BC0F630E3E8EB01" modified="2023-11-08" version="1">MGRGARPATQLPVELRPVNVYTPNTLSNLKQPIMLSSSTICASCLRAARRGVNNSVAETARTVRPTTRAYSRATSAATWRPIHPEFLAPSAIKADSRAISAFNPRAPLRTKSQHASRRTLATTSDPSSAKPAHAIQNPRTDDSGNPMLISISPRASNRLYSISTKDNNPNLALRVTIESGGCHGFQYLMDLTDLSKKPATEEDTIFEGDKGEKIIIDEPSLELLNGSTVDYTMELIGSQFKVTGIPGATSSCGCGTSFDIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGD0</accession>
    <name>A0A9W8YGD0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGD0"/>
    <dbReference type="OrthoDB" id="3594103at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37538">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37538:SF4">
        <property type="entry name" value="PITSLRE SERINE_THREONINE-PROTEIN KINASE CDC2L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="41428" checksum="E6394DE7690FC86A" modified="2023-11-08" version="1">MKDVGPNATRFDIHGTILRQSAELAAKFDPWSQKKEPVPLPELDEAAAHTLVHYLYSGKYQALNTHVQPEKAVALSYKLGTCVYCAAVRYKLPGLVELAKDKITSLGEDVSIFDTLAMAKDHAFPLLPDEETWYPAYLEGAIISAMAEDPEPFRKPDFITQVEGNSRLLQVVWKTVMNNYAKIPVTPLAAEDDVATPTAESAPEEPAPVIVHDIEPVLEAEDASSGEAELVQNVASASTSIEAEASPQVAVSESDNTQGDALELDDIEPTVETRQAPEPFTDELGFETSKMYKQMGKKSDFALATETTLHEEPKKTAHTRSDSVMQSEEAEPRPELERKDSEAVEVEDNGSQVVNGANDGATPSKKNKKKKSKKSSIVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH88</accession>
    <name>A0A9W8YH88_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_000303</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH88"/>
    <dbReference type="OrthoDB" id="419598at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018744">
        <property type="entry name" value="DUF2293"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051609">
        <property type="entry name" value="NmrA/Isoflavone_reductase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47706:SF7">
        <property type="entry name" value="CIPA-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G01630)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47706">
        <property type="entry name" value="NMRA-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10056">
        <property type="entry name" value="DUF2293"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2293" evidence="4">
        <location>
            <begin position="82"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="NmrA-like" evidence="3">
        <location>
            <begin position="206"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4377478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="56239" checksum="36DBA6E048793479" modified="2023-11-08" version="1">MPKGYGFLAKGIRYKTLHCRKLTHEAKKILYIVVDKKKQVGLRVPLSILHQVHAQAKQTLPTRRAATEKRDAADIGKAAAELHSQFPKIPEKDKEKVLKHGFKKHSGRVGRTSSIPLSRKVLLAVIAHVRHQHTTYDTLLSGGEDRDMARKKTRKQIETIMREWGFKEALNKPIHENITFTETALACPPTPSLHYKRLAMSNNQIKNIAIVGASGNIGSQIATALLAKGQFNITAISRAESKGTFPQGIKIAHIDYNNPDTIVKALKGQEVLIITMGVLAAPDTQTKLIRAAAEAGVPWVLPNEYGMYTSEEVQNELIGPGKAKDRQLIEELGVSSWIGVVCGFWYAHSLSGPGLYGFDIAKREAVFFDEGTEKLNTSTFPQVGRTVAALLSLPVSSQGSTEGGPTLSDYRNSRVYTSSFALNQLQFRRKNAMLKPGEQLKGGNRMAFGKVLYTRYFFPGDYAGLYEKIHGLDNEKLGLPQEDLDEATAEVEARGERLLETIRKTLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH97</accession>
    <name>A0A9W8YH97_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Niemann-Pick type C- protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">NCR1</name>
        <name evidence="18" type="ORF">N0V83_000814</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the patched family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH97"/>
    <dbReference type="OrthoDB" id="6510177at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015918">
        <property type="term" value="P:sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1640.10:FF:000008">
        <property type="entry name" value="NPC intracellular cholesterol transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1640.10:FF:000029">
        <property type="entry name" value="Putative Patched sphingolipid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004765">
        <property type="entry name" value="NPC1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053956">
        <property type="entry name" value="NPC1_MLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032190">
        <property type="entry name" value="NPC1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00917">
        <property type="entry name" value="2A060601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727:SF2">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22314">
        <property type="entry name" value="NPC1_MLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16414">
        <property type="entry name" value="NPC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="12" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040752548" evidence="16">
        <location>
            <begin position="27"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="271"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="356"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="600"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="637"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="667"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="719"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="744"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="828"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1075"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1102"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1131"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1175"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1216"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="17">
        <location>
            <begin position="595"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4377984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1276" mass="140768" checksum="6873BC9C0ED4676A" modified="2023-11-08" version="1">MVQPCSRGWRAITVGLFSILAGSALAGPDLTSKHEKGRCAIRGHCGKQGFFGSELPCPDNGPAKTPDDAVRTKLVDICGEQWSDTDVCCAEEQLDALKTNLDRATPIINACPACKENFYNMFCTFTCSPDQSTFINVTQTEPKGDNFLVTELDNLVSDKYASTFYDSCKDVKFGATNGKAMDFIGGGAKNYTQFLKFLGDKKFLGSPFQINFPRPDEDKFPDMEGMNKHAYPCNTTDEKYRCACLDCGGSCTELPEVQEEKQCYVGLLPCLSFAVIIIYSFFLALLCAAVAGHVAYQNHSKHKNERMRLLQDLEPSDDEDEGDIVHNIGMLDRPTKHYFVNTWCDRMFNRLGYVCARFPAITIATSIVVVALMSLGWMRFEVETDPVRLWVSPDSAAAQEKAFFDEKFGPFFRAEQAFLVNDTLEGSGPVLSYETLDWWFGVESQIQRLKSYGSGVTLDDVCFKPIGDACVVQSLSGYFQGDFANVSPNSWREDLQDCVDNPTQCLPTFQQPLDPHLLFGGVNGSVLDAKALVVTWVVKNHPKGTPEEQRAMDFENELKNYLMFVSEDARSRGLRLSFNTEVSLEQELNKSTNTDAKIVVVSYIIMFLYASLALGSTTLTVRSVLRNPANAFVQSKFMLGIVGILIVLMSVSASVGLFSAAGIKVTLIIAEVIPFLVLAVGVDNIFLLVHEFERINISHPEQTIPERVARALGRMGPSILLSALTETTAFALGCAVGMPAVRNFAAYAAGAVFINAILQVTMFIAVLALNQQRVENLRADCFPCVRVWRADPGYLNGGMGHGAGEEGGLQRFIRKTYAPAILGKKTKVGIIAIFFAIFTAGIALFPSVELGLDQRIAIPSDSYLIPYFNDLYDYLDVGPPVYFVTKELNITQQKPQKELCGRFSACDRGSLANIIEAERKRPEVSHLAASAANWLDDYFLWLNPQNENCCVDDNGKPCFQDRQPPWNMTLSGMPEGEEFIHYLQRWVEAPTTEECPLGGKAAYSDALVIDSKHLTIPASHFRTSHTPLRSQKDFISAYTAARRISREISQDVETEVFPYSKFYIFFDQYTTIVRLAGALIGSALAAVLVITFILLGSLATALVVTLVVGMTVSTIIGSMAILGVSLNAVSLVNLIICVGISVEFTAHIARAFTFPSRPTMEKAPRHRFRGRDARAWTAMVNVASSVVSGITITKILGVGVLAFTRSKIFEIYYFRVWVALVLWASTHSLILLPVLLSLVGGKGYVDSESEGGLEQDLRSRSYPALLAEDEEYDSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YI64</accession>
    <name>A0A9W8YI64_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Putative glutathione-dependent formaldehyde-activating enzyme</fullName>
            <ecNumber evidence="5">4.4.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">S-(hydroxymethyl)glutathione synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_000803</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the condensation of formaldehyde and glutathione to S-hydroxymethylglutathione.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>S-(hydroxymethyl)glutathione = glutathione + formaldehyde</text>
            <dbReference type="Rhea" id="RHEA:22488"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58758"/>
            <dbReference type="EC" id="4.4.1.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">One-carbon metabolism; formaldehyde degradation; formate from formaldehyde (glutathione route): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the Gfa family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.22" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YI64"/>
    <dbReference type="OrthoDB" id="3446116at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051907">
        <property type="term" value="F:S-(hydroxymethyl)glutathione synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1590.10">
        <property type="entry name" value="glutathione-dependent formaldehyde- activating enzyme (gfa)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00723">
        <property type="entry name" value="Formald_GSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006913">
        <property type="entry name" value="CENP-V/GFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014185">
        <property type="entry name" value="Formald_GSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02820">
        <property type="entry name" value="formald_GSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003829">
        <property type="entry name" value="PRK05417.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33337:SF40">
        <property type="entry name" value="CENP-V_GFA DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33337">
        <property type="entry name" value="GFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04828">
        <property type="entry name" value="GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033318">
        <property type="entry name" value="Formald_GSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51891">
        <property type="entry name" value="CENP_V_GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CENP-V/GFA" evidence="6">
        <location>
            <begin position="19"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>structural</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4377973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="190" mass="20530" checksum="5C788E7661A1C43E" modified="2023-11-08" version="1">MLSIHPSIDNGITKGDSNFSGGKLYCRCPSNKVEVTLGSNVLHNHACGCSKCWKPEGALFSVVGVIPVDKVSVTANADKLHIIDSSAAIQRNACKECGVHLFGRIVNDHPFKGLDFVHTELSDSKGWQEPQFAAFVSSIIEQGFRPEGIDGVREKFKSVGLETYDVLSPPLMDLIATYTGQKSGKLPAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YII1</accession>
    <name>A0A9W8YII1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001516</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YII1"/>
    <dbReference type="OrthoDB" id="3796480at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="206" mass="23465" checksum="AB5F02E553128AFE" modified="2023-11-08" version="1">MSGLSLEDFDKHIEALRTQLQFLNESLEATDHHAPDWLATDLISLRNKSGRLQDDMKRFRDQLDSEGLAVKKNQAVSPPLAQSPTPRGKPVPAEEDTYVVQHIDVTEEVSRRLRESRLRRLMDTPSTAQKRKYNAYEDQGTESAVDAEDDGSRAGCSGSEYERTPTKRLKSSGTFEQVGKRKENGNERPSEEERFGSRADFKRRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHM4</accession>
    <name>A0A9W9CHM4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010066</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the paxM FAD-dependent monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHM4"/>
    <dbReference type="OrthoDB" id="16820at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000115">
        <property type="entry name" value="Salicylate hydroxylase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050493">
        <property type="entry name" value="FAD-dep_Monooxygenase_BioMet"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13789:SF306">
        <property type="entry name" value="HYDROXYLASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13789">
        <property type="entry name" value="MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="7">
        <location>
            <begin position="80"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4362949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="505" mass="55938" checksum="73DD8A32972C438F" modified="2023-11-08" version="1">MPTATEQKDDTNATIGNGYAYNISSKKKSSHAVIPHWEPSLIASYPSQSLRFLKKARKETGSSQAPHLPSASTQARLKLNIVVVGAGLGGLALSIALARRGHSIRVLEQATKLGEVGAGIQIPPNSGRLLHSWGVFEHLEEWVVKPEAINFRRWENGQEIGRTALGEAFQDNFEVPYYVVHRAHFHNALHQRALQLGVSIQLGARIVRYNEKNASVELSDGTVVQGDLVIAADGIKSIARETLCPEISHELQFNGFAAYRATVDVKKIREDPEIAWIVEKPSLNIWIGENRHVMTYTIAAGETFNMVLSHIDQSDPATWTKKFAKEDIQQEFSGWDPHLTKIISLIDDCLKWPLKVGHQLKTWTCPSSKLIIIGDAAHAMLPYMSQGAAMAVEDGAALAVALNHISSLEELPFALRTFEKERIKRSSDMQNASTINGTIWHFADGPEQRARDESMAAEVAGLPVLSSANQWTDPVTQWWAYGYDAEEAMEKAWSRAVSDVITQLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHT7</accession>
    <name>A0A9W9CHT7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Folic acid synthesis protein FOL1</fullName>
            <ecNumber evidence="10">2.5.1.15</ecNumber>
            <ecNumber evidence="12">2.7.6.3</ecNumber>
            <ecNumber evidence="11">4.1.2.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Folic acid synthesis protein fol1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">FOL1</name>
        <name evidence="26" type="ORF">N0V83_010100</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="26">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Catalyzes three sequential steps of tetrahydrofolate biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate = 7,8-dihydropteroate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19949"/>
            <dbReference type="ChEBI" id="CHEBI:17836"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="EC" id="2.5.1.15"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde</text>
            <dbReference type="Rhea" id="RHEA:10540"/>
            <dbReference type="ChEBI" id="CHEBI:17001"/>
            <dbReference type="ChEBI" id="CHEBI:17071"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="EC" id="4.1.2.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the C-terminal section; belongs to the DHPS family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">In the N-terminal section; belongs to the DHNA family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="22">In the central section; belongs to the HPPK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.15" evidence="10"/>
    <dbReference type="EC" id="2.7.6.3" evidence="12"/>
    <dbReference type="EC" id="4.1.2.25" evidence="11"/>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHT7"/>
    <dbReference type="OrthoDB" id="615426at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
        <property type="term" value="F:dihydroneopterin aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004156">
        <property type="term" value="F:dihydropteroate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00739">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00483">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.20:FF:000006">
        <property type="entry name" value="Dihydropteroate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.560">
        <property type="entry name" value="7,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="entry name" value="Dihydropteroate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045031">
        <property type="entry name" value="DHP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006390">
        <property type="entry name" value="DHP_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000550">
        <property type="entry name" value="Hppk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035907">
        <property type="entry name" value="Hppk_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01496">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01498">
        <property type="entry name" value="folK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941">
        <property type="entry name" value="FOLATE SYNTHESIS PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941:SF1">
        <property type="entry name" value="FOLIC ACID SYNTHESIS PROTEIN FOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01288">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55083">
        <property type="entry name" value="6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="Dihydropteroate synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00793">
        <property type="entry name" value="DHPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="19" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="16" id="KW-0418">Kinase</keyword>
    <keyword evidence="18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pterin-binding" evidence="25">
        <location>
            <begin position="171"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00005051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00009640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00009951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00012458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00013043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00013253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00058009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00061548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00067568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00068111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAJ4362983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="443" mass="48619" checksum="A2B2B0342576AF4A" modified="2023-11-08" version="1">MGDRVAMIEQACNEMEASGRIRIKRTSSLWETKAMYVLDQDKFVNGACEVETSLSPIELLDELQAIENKMGRVKVIEKGPRNIDLDILLYDDLTFSNERLQIPHLLMLEREFVLRPLCEIVSDEVRQCLPITGSLQQHLSRLPLSEEPLSPMTPLSPGLSHISTYQPHRDTRIMSILNVTPDSFSDGGKNYDIDESSLADTIKSHIAAGATIIDVGGQSTRPGSVQVSSEEELSRVLPAVKLIRALPEAKNVAISIDTYRGDVAEASIKAGADIINDVSAGLMDDTMLPTVAKLGCTICLMHMRGTPETMNSLASYPDGVVETVGKELLERVRAAEEAGIRRWRIILDPGIGFAKKQGHNLELLRRQGDLRKYPGLEGFPWLVGTSRKAFIGRITGVKEAKDRVWGSAAAVTAAIQGGADIVRVHDVQEMGQAAKMADAIWRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHZ8</accession>
    <name>A0A9W9CHZ8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">GPI ethanolamine phosphate transferase 1</fullName>
            <ecNumber evidence="14">2.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">MCD4</name>
        <name evidence="17" type="ORF">N0V83_010312</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 14">Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the first alpha-1,4-linked mannose of the glycosylphosphatidylinositol precursor of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the PIGG/PIGN/PIGO family. PIGN subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.-.-.-" evidence="14"/>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHZ8"/>
    <dbReference type="OrthoDB" id="2748310at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16020">
        <property type="entry name" value="GPI_EPT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000015">
        <property type="entry name" value="GPI ethanolamine phosphate transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007070">
        <property type="entry name" value="GPI_EtnP_transferase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017852">
        <property type="entry name" value="GPI_EtnP_transferase_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037671">
        <property type="entry name" value="PIGN_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250:SF0">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN, CLASS N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04987">
        <property type="entry name" value="PigN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 14" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="472"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="501"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="523"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="562"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="587"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="616"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="640"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="685"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="714"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="744"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="835"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="874"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="909"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="940"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="domain" description="GPI ethanolamine phosphate transferase 1 C-terminal" evidence="16">
        <location>
            <begin position="452"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4363192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="993" mass="110980" checksum="508E0B8D8EBFDD5F" modified="2023-11-08" version="1">MAQFGRYRFLVVAVIFHLVYIYSIFSCYFVSPIVHGMREHRVETQEALAKRLILFVGDGLRADKAFQSFPNPDPKTPTTLADDPTEPRPLAPFLRSRVLESGTFGVSHTRVPTESRPGHVALIAGLYEDVSAVTTGWKLNPVHFDSVFNRSRHTWSWGSPDILPMFEQGAVPGRVDAYCYEAEFEDSSKDAWVLDDWVFARVKKLFEDAKTNATLNAELRQDKNVFFLHLLGLDTTGHAHRPYSWQYLHNIQIVDRGVQEITRLIEDFYDDEKTAFVFTADHGMSDWGSHGDGHPDNTRTPLIAWGAGVATPVKNTSGIAPGHDEYSSDWNLDEIERHDVDQADVAALMAYLVGLAFPTNSVGVLPLSYLNMGAKAKADALLANAKEILEIYHVKEELKRSTELRYKPFPRLGDEAHSVEHRVSQIQKDIDDGKEEEAIRQTYELIDLGLAGLRYLQTYDWLFLRTLVTAGYVGWIVFAITTVIDLHVLTQDVPALRTTESIAIFSGLLVALYAVLLKQQSPVIYYVYAFFPILFWEEVYARRVSLVKGSKILFGHVSSNSDVAKLVLSTLGFFGLLEALVQSYFVREIYTICYLFATCWPAVYGQDFIRKNLGTVATWVLSCIIMSAFTMLPANKAEDTRLILLGGCLMFAVGALYLLFEKNILADAGSDDELAEPKLDYKSRIILSVQLGLVLLSMIVTKSSIGYIQARQGIPLGVLTVARATLLVSLLLPQLHSFYPNSHYIHRLVVIFLQFAPTFIILTISFEGLFYFAFCLCIFSWMRLEHKIYLHTDKTGKTGSASQNPIKPAVEAAANRLDSLQKGDYRSLTLADARIALFFFFFIQSAFFSASNIASVSSFSLDAVYRLIPIFDPFSQGALLMLKLMVPFAALSANFGLLNRRLGVAPSALFMVVMAVSDVMTLSFFFMVKDEGSWLDIGTTISHFVIASLLGIFVAGLEIYSEWTIKGVEFRDGSKAANKVHGSTEGKANGQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIR1</accession>
    <name>A0A9W9CIR1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIR1"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="145" mass="16629" checksum="07C1C2C44FC21BD1" modified="2023-11-08" version="1">MNALRPLLVRRVSAVTSPVVTPPIVCPIRFFSRSRHTREFDPLLRKLLNQSEQHSEILREIIQNSKVLEAKWDLKFQALEKDGKMHMQTLDEDGKMHKQTLDERLNVFEVGRDLNRSATRTLFAITSVVFSGSSVLVAWWGLTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIS0</accession>
    <name>A0A9W9CIS0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">S-adenosylmethionine-dependent methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.297</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MTQ2</name>
        <name evidence="4" type="ORF">N0V83_008431</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.297" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIS0"/>
    <dbReference type="OrthoDB" id="406152at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035657">
        <property type="term" value="C:eRF1 methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102559">
        <property type="term" value="F:peptide chain release factor N(5)-glutamine methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000274">
        <property type="entry name" value="ERF1 methyltransferase catalytic subunit MTQ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052190">
        <property type="entry name" value="Euk-Arch_PrmC-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875:SF1">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="254" mass="27897" checksum="598A5DD7C17936F1" modified="2023-11-08" version="1">MLPTPSTSHICFDRVYEPAEDSYLLLDTLSSPSETAFLRGRFKHAASTPPLVLEVGVGSGVVLAFIAANTQTIFGRKDVMTIGTDINSFACCAASQTVRNAIKEMEKEPSVFLDVVNGDLATAIRPYSIDVFVFNPPYVPAELPDLSRHDDYNKITEGKEATSFEQDSYLLELSYAGGEDGMVVTNRMLEQIPDILSEERGVAYVLLCAQNKPEVVKAQIRGWGPNWNAETVGSSGKRAGWEKLVIVRIWKTAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIT5</accession>
    <name>A0A9W9CIT5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CBM20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_009142</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the polysaccharide monooxygenase AA13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIT5"/>
    <dbReference type="OrthoDB" id="550577at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001070">
        <property type="term" value="F:starch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05811">
        <property type="entry name" value="CBM20_glucoamylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000552">
        <property type="entry name" value="Related to glucoamylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034836">
        <property type="entry name" value="CBM20_glucoamylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004302">
        <property type="entry name" value="Cellulose/chitin-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052282">
        <property type="entry name" value="Starch-active_LPMO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36575">
        <property type="entry name" value="BINDING PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G14430)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36575:SF2">
        <property type="entry name" value="CHITIN-BINDING TYPE-4 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03067">
        <property type="entry name" value="LPMO_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51166">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040787619" description="CBM20 domain-containing protein" evidence="11">
        <location>
            <begin position="18"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="CBM20" evidence="12">
        <location>
            <begin position="290"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="252"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="253"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4364546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="396" mass="41844" checksum="A052A7EF72336B80" modified="2023-11-08" version="1">MRSSLVQLLSFAVGIHAHGYLSSPMSRTGLNAQAGADTCPECTILEPVTAWPDLDSAQVGRSGPCGYNARVSVDYNQPGPNWGSQPVITYTGGSTVDVQWCLDNNGDHGGMFTYRICQDQALVDKLLTPGYLPTDAEKQAAEDCFRAGELKCTDVVGQTCDYNPDCQEGQACWRNDWFTCKGFNDGAKCRGVDNAPLNSCYTSIAGGYTVSSKIKIPNYVSNHTLLSFKWNSYQTPQIYLTCADIAITGSGGSVPPTSSKAVTSSTKAATSSTRTTSSTVPSSAASSACATPVPTVAVTFNSKTTTSFGQTIKIAGSISQLGSWDTANAPALSASQYTASNPLWTTTINLPAGTSFEYKFIKVESSGAVTYESGANRAYTVPKSCSSAATVDTQWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIX5</accession>
    <name>A0A9W9CIX5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009263</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIX5"/>
    <dbReference type="OrthoDB" id="20872at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="105"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="204"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="271"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="408" mass="43498" checksum="8E87EF4FAE882E80" modified="2023-11-08" version="1">MMSAIFHAAMNGKTIEVSMLLSRNEIHLDQQDTSGRSPLSLAAGNGHEAIVKLLLDTGKVKIEGKDNNGLTPLSWAAAKGQESTVQLLLDTGKVYVNSNNSMGGTGETPLFRAVRYGHEKVVKLLLNTGSINIDGAWHCQTLLLCAALNDHEAIVKLLLSTGQIDVDATKNGKTPLLAAVLNGNERVVSLLLDTNQVDVNAEWHGSTPMIAAASAGNEALVTLLLSTGKVQVDKESKKNTPLLSAALNGHVAIVELLLNTGQVDIEATDLDGQTPLSLAAMRGHERVVRSLIRFGGRVDSKDLSGRSPLLCAAMNGHEPVVKTLLSASVINVDAPNHRGQSPLFLAAAGGHEAIVKLLLATDEVDVRRTDKYGQAASSGNMAVVELLQDAMNGRTHIQRQVKWPWKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ25</accession>
    <name>A0A9W9CJ25_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GRIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_008615</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4364999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ25"/>
    <dbReference type="OrthoDB" id="425925at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019459">
        <property type="entry name" value="GRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000237">
        <property type="entry name" value="GRIP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18921">
        <property type="entry name" value="MYOSIN HEAVY CHAIN - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18921:SF2">
        <property type="entry name" value="THYROID RECEPTOR-INTERACTING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10375">
        <property type="entry name" value="GRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50913">
        <property type="entry name" value="GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GRIP" evidence="6">
        <location>
            <begin position="449"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="497"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="557"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="315"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="106"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="521"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="557"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4364999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="590" mass="66108" checksum="BBF2F4F88434822A" modified="2023-11-08" version="1">MSAPATSDPITQSGATSKSKKKKNKKKGGGGANKAEEPEKANGNLSKPEAEDHEEDAEEDDEQIKPAAQPADPEADDVDDERPLSPTLIQTNGTHHHSIASAVALGLSRSRSQQGLSPSPPPPSDTSARLDAIAKERDALRQEVTELRKSLEIIQSKHEAAPAEDVSSKHEEEIRNLREELDEANEGREHFETQYKNLLGRVNTIKTSLGDRLNADKARIEEYETQVAELEEQTRELQENNNTLSEDLAKLRQERDAQASEVENLRSRSNLSQQNWIKERDELISREAYAREEFENAKQAMQDWEVLAMNERSLRESLTEKDTELRDQLESFKEEYEKAARDRDTNSQAVEGLQKALQEVQTLRKAELKKSVETYESQINDLRKQVQTAEEASKSAKATVQSIQKELERALPFEKEVKEKNLLIGKLRHEAVTLNEHLTKALRILKKGRPEDNVDRQIITNYFLHFLSIDRSDPKKFEALQLISALLGWTDEQREQAGLARPGTSSTSSGLRIPLSPFRRTPSTPSLNSISDPMLMASSSSNKESLAELWQDFLEREAAEGKDTSKSRRGSAAVSPPPRTGSGLGISEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJM5</accession>
    <name>A0A9W9CJM5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">vesicle-fusing ATPase</fullName>
            <ecNumber evidence="3">3.6.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">VPS4</name>
        <name evidence="16" type="ORF">N0V83_008343</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.6" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJM5"/>
    <dbReference type="OrthoDB" id="29072at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19521">
        <property type="entry name" value="RecA-like_VPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000004">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000043">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000015">
        <property type="entry name" value="vacuolar protein sorting-associated protein 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF83">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04212">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MIT" evidence="15">
        <location>
            <begin position="3"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="14">
        <location>
            <begin position="163"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="69"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="416"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4365723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="433" mass="47639" checksum="0E8470515DFD18AC" modified="2023-11-08" version="1">MSNADFLGRAIEQVKKAIETDTAGEYEKAYQLYYSARDRTGEKNQKSKEMIRGKVAEYMERAEKLKQHLNQNDASNRKKPAAMGSNGKAAGGSGKGGKDDDEEGEIDADSKKLRGALAGAILSEKPNIRWEDVAGLEQAKEALKEAVILPIKFPHLFTGKRQPWKGILLYGPPGTGKSYLAKAVATEANSTFFSVSSSDLVSKWMGESERLVKQLFGMARENKPSIIFIDEIDALCGPRGEGESEASRRIKTELLVQMDGVGKDSKGVLILGATNIPWQLDSAIRRRFQRRVHISLPDTPARMRMFELAVGNTPCELNQADYKKLAELSEGYSGSDISIAVQDALMQPVRLIQTATHYKPVEVDGTTKWTPCSPGDPQAQEKSWTDLDGDQLLEPPLKVKDFIKAIKASRPTVSGEDLKRSADWTKEFGSEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJV3</accession>
    <name>A0A9W9CJV3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SGT1-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJV3"/>
    <dbReference type="OrthoDB" id="27237at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010770">
        <property type="entry name" value="Ecd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13060:SF0">
        <property type="entry name" value="PROTEIN ECDYSONELESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13060">
        <property type="entry name" value="SGT1 PROTEIN HSGT1 SUPPRESSOR OF GCR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07093">
        <property type="entry name" value="SGT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="558"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="635"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="475"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="565"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="580"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="648"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="657" mass="73861" checksum="B5506057F35DD540" modified="2023-11-08" version="1">MDYPQDDLKWFGEGFDGFPKKLPDDVVEYIIFIIDTKLSDIQIRERLQAFQRALNTLEKKFLKEYIWQRDSISLELVREEQRWLLRGRTNYGDSVADEWLIVYFLRELSKEFKDAWIRIYDSDGEFLLIEAANTLPKWLNPEVAENRVWINTHRLLIIPLAKEEEPGPLKQVQALRIIEDTPARPQHYPKVEREAFNRLNGYPAAIAENQHHATLPLPRKVAHILHISPSYIAPVVEAFYLRDPVSTRLLQAEQSKTPLAFPPEDFVDVSVRFTKVLYAQLLGQQWSPPAPWDAALESLIKSGKPSEKAEVGIKVAAGMQMLVSHSLYASRRPTREITLLLEDLETGDDALPTDVEISRWPKRDDDEGWLNIDFSEFEKELEGKGGKEEAFGDKNAQENLRKMVERFNSFLADNEAGIEGADGKPDPMDEDNDSDAAERAWDDPEDSESEQGEIDEGKTESKRTTHSSAKTGGEDAGEDSDDAEADAEFAEYEAAYAKFMTLSAAEKALLTDEARELALAQDAEKEEDEEIQKLTEMMEAELFSHGALNLNPKPARKFGQGLLGGDEKAKGKGKAKLEEIDAEGYGEGDDDDDEDDDDLLDEDYNLADNMLKAFKGQAGMAGPAGNMMKLMGVQFPQDKDDEVKGSGSSTGKGKGKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK04</accession>
    <name>A0A9W9CK04_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK04"/>
    <dbReference type="OrthoDB" id="6079484at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="147" mass="16771" checksum="34BFF989D4F90CA3" modified="2023-11-08" version="1">MTFGVRWEFGPGDAGTEHNLDFHILKGAPADVILSDEFIFGTNEFIEYDCYLVDEDEDDEETDAYFFAIQIDKSHKTSVPVPEQLREDVYRSTQDDEIDKLPLHEQAAARQKEETRRAEWDVQRNLTFDEGESLPGGTSLGPLTLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK07</accession>
    <name>A0A9W9CK07_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007893</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK07"/>
    <dbReference type="OrthoDB" id="5215637at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="161"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="368" mass="37632" checksum="D57698237C8B1F15" modified="2023-11-08" version="1">MVRPDMTASGGESITQCSGHDNDWCCNADAQHVNCCKESPSPRPFFQLQDGKAYATVGSSTASSVPTISSITGLASGTGTAGSASQTSAAASSGGSSAGSRAVTADATSASASPYTSLSASVTSDAGGVRTVYNTLVITPTSDPTAAAGSSSTGSKSKSNLGLIIGCAVGIPLVLAFLGIAIWLLRKRRQQKAANPYKETSEIDDNSPNSPGFIGGAAGKLGKKQTFRSSRPGTAEIDGNPVGPGRPVSTVKGHAELESGSGFRPGQNAPYAPDTVGLGGGNGAERSTWGSVPPQYSPAHNQTGFTHNHNPEATELDGTTAMPVINEKAENEPQYIAYRPPQSQQPPPAELPTVKTPPEDLEKQIHHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK20</accession>
    <name>A0A9W9CK20_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Alpha-actinin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ain1</name>
        <name evidence="7" type="ORF">N0V83_007494</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alpha-actinin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK20"/>
    <dbReference type="OrthoDB" id="10017054at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21215">
        <property type="entry name" value="CH_SpAIN1-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21291">
        <property type="entry name" value="CH_SpAIN1-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000138">
        <property type="entry name" value="Alpha-actinin, sarcomeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000097">
        <property type="entry name" value="Alpha-actinin, sarcomeric (F-actin cross linking protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000279">
        <property type="entry name" value="Calponin domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000223">
        <property type="entry name" value="Related to alpha-actinin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000030">
        <property type="entry name" value="Related to alpha-actinin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000077">
        <property type="entry name" value="Related to alpha-actinin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014837">
        <property type="entry name" value="EF-hand_Ca_insen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08726">
        <property type="entry name" value="EFhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01184">
        <property type="entry name" value="efhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="10"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="124"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="484"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4366964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="638" mass="73352" checksum="22EEB88DCF60C6B5" modified="2023-11-08" version="1">MAPLEQQWVKVQQKTFTKWLNSKIKTRQVEIQDLITDLADGIVLIHLLEILSNESLGRYASRPKLRVQKFENVNKSLDYIRSRGIQMTNIGAEDVVDGNSKIILGLIWTLILRFTISDINEEGLSAKEGLLLWCQRKTACYDEVEVRDFSTSWNDGLAFCALLDIHRPDLIDYDSLDKNDHRGNMQLAFDIASKEIGIPDLLDVEDVCDVAKPDERSLMTYIAYWFHAFSQMERVENAGRRVEKFVNNMQGAWQMQNDFERRMRELLGQIAKQRTQWVEAKFDGSYADARNQSTEFTAWKRKSKRTWVAEKSDLVGLLGNIRTKLATYRLRPYDPPEELSLEALDAAWTGLMKAERQRSQVINETIRDIKNALRKSFADKANDFALALNTLSTSISALEGDVEDQLEHTKEISKSLAPLDEYLNLIASIDEQCAEANIEENDFTTYTYDELEYELSLVRSSVSKKLAFLENQMVARNMTNLTPIQLEEFESVFRHFDRDLSNSLAELEFSAALASLGLVYDEAEMHQRFRETSGGKDYVTFEQFIRFMVDETEDQNTAEQVFESFKEVADGKPYVTELDLRHSLVPDEVIEDLLATMPTHKGPDMQEDRDIPKFDYITYMQRYMGADRGTGNGQVNGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKA6</accession>
    <name>A0A9W9CKA6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">General transcription and DNA repair factor IIH subunit TFB4</fullName>
            <shortName evidence="14">TFIIH subunit TFB4</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 14">RNA polymerase II transcription factor B subunit 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TFB4</name>
        <name evidence="16" type="ORF">N0V83_006883</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 14">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the 7-subunit TFIIH core complex composed of XPB/SSL2, XPD/RAD3, SSL1, TFB1, TFB2, TFB4 and TFB5, which is active in NER. The core complex associates with the 3-subunit CTD-kinase module TFIIK composed of CCL1, KIN28 and TFB3 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the TFB4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKA6"/>
    <dbReference type="OrthoDB" id="17307at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000084">
        <property type="entry name" value="Transcription factor TFIIH subunit Tfb4, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004600">
        <property type="entry name" value="TFIIH_Tfb4/GTF2H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12831:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12831">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH TFIIH , POLYPEPTIDE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03850">
        <property type="entry name" value="Tfb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="346"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="359"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU368090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4367302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="377" mass="40023" checksum="74925F079ED96F25" modified="2023-11-08" version="1">MNTIDGSNRHIKTDEAPPPSLLAIVLDTNPHAWAHLSSTLPLSAALANILVFINAHLASGNANEVAVIASHSHKTAFLYPTPDPPQPKTRHGTANGHVETNGVGADREQHLIESANKYRPFAVVEQAILRNFSKLLDETTESHLQATPTTLIGGALSLALTYINKSTILHAPTAASAESLSLAALADSENSHVDHVPLTSRILIVSVSGDLANQYIPVMNSIFAAQRKKIAIDILKLAGDTVLLQQASDATGGVYMKPERPEGLLQYLMMAFLPDATARTSLVVPSAGGVDFRAACFCHRKVVDIGFVCSVCLSIFCEPNLPDNLCLTCGSYLSLRSAVMNPPALIPRKKKKKKKAGTDSATPGANTPAGSSTPMHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKU6</accession>
    <name>A0A9W9CKU6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006515</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKU6"/>
    <dbReference type="OrthoDB" id="3045089at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054464">
        <property type="entry name" value="ULD_fung"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38886:SF1">
        <property type="entry name" value="NACHT-NTPASE AND P-LOOP NTPASES N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38886">
        <property type="entry name" value="SESA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22893">
        <property type="entry name" value="ULD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="748"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="537"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="537"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="557"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="825" mass="93572" checksum="1DC9F5A1306FD40B" modified="2023-11-08" version="1">MPITFGAVGDIISVCLLVKDLVETLDKARGSRPEYQSAIRELWILDRALLEVDLLTRLHGNGATPELRGLCETAKQAATRCKDLISRFHDRIRKYKSSFDEGKSPSIIKNAALQIRWRLEEKDALDQLRVEVAGTSSSLQMLLATASVTLMGISKKQLNDRLDEAKEQTDTKNNQQSAALLTIQSRLEDANRQIESGNSLLGKVSDALRLDWLRQLGSELKALMRRAIATNIATYHAVLSIQAVLPSRLERGMIEEPFILEDPIGRIAPVHLQFVTSWDAFNAILEVRFRDIQGYRKIKQRKYGLQDKSTGRDILQTRRWGGAFLPGQRVEMSLLFETKGSTVNDDAQVSCPGCQATSVGGNEADIQCERCGMWFRRITVIREIEPPPPVPLPKPWTAKPEFGKSTLAFQLVGPLKPGRKRTSPDDLEGEEEAREFKRVRIIQAKEQIKVRKFGQNGQGNEYTTFSKLQIPEPAKKTLWRDSKTLDAALSATSHLSELEKFRQQMSVLKPRSDNKNVGEALEQVSLSRAIFADPADLEPKPKFQETSTRESDEIAQSEESSPFPSEFSRRNAIDMGGLPRYQRPLFSTGPLFEWKYASTITQELQDVYRPRAPPVDLRLYDLLELPYWGVPTSIIVRAYKHRVRECLSCGSLNTACAAAAEQLNGVLDLVLETLTNARKREEYHRTGRVTSNLLEHLFPFIQDAVSSGRLVEAVEKLCRCGTPCRKVWMIWEIEDVQQVDIRSIKPWYYYSVLGLPPDTTQEQIRREYRLLAIRNHPDKNRDDEAASKRFQEISEAYEILGDELNRSLYDVTGAIPRHLRKKISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL70</accession>
    <name>A0A9W9CL70_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006398</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL70"/>
    <dbReference type="OrthoDB" id="5392263at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="287"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="493"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="538"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="582"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="611"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="622" mass="70252" checksum="C832B80969C41EE2" modified="2023-11-08" version="1">MVDMAVRYDETPDRRTDFGSMRDSMYLLLVMNQYTLKQTAVLREKEAEGLLRIVLFSKDLKLTDTDKTLRQMRRILAREVREMRRRGAVPAAFGDLGENATAHDLALGCLLAWFPILIMGSIVDRNPIAAEAIRKKLNALVDHVRHALRDEQHRKAFIDTFLDQPECDQLKIWVKNVAEKGEHMYDFFVDFAGQARIRWHYGAAHPILSDIENCYIADKGRNWLANEGEARANLVIGPVNDEGLVWFDIREFWQVASAIIIVAGSCGGAFVLSYFTPTVGLGCRSGGYVIFFSVALGLLIVEMTVYIVLAQTCGWYKTCECVTSTWGGAGGYLDFSVQDTSNSEGVPYYWASGTVLTALIMGLAMFYITVEWCQQSFLSTEDYADAMDGLRKTRTYRHWTFFLRWLSRQLCRWSLCPMERLAVIVGLIKKQQKTLLWTKHHTWDPIVPPPQRQITTTEPIYPNASPSIELTDYSTNAPHTEMFVHDTPAMAHSLFPPAIPTRRPRNESDASLLPPNRTSDDSSAPLIQRPSETYHQGAEPECRRSGEGRVSFEEAWSPASPPDAGPAYDRWRVPDGMLHSRQGYQRANSDPGSPPVEIRNMAQSGLGIHVRNTDSDLERGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLC6</accession>
    <name>A0A9W9CLC6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006549</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLC6"/>
    <dbReference type="OrthoDB" id="47007at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43639">
        <property type="entry name" value="OXIDOREDUCTASE, SHORT-CHAIN DEHYDROGENASE/REDUCTASE FAMILY (AFU_ORTHOLOGUE AFUA_5G02870)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43639:SF1">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="35552" checksum="C8D45EA39D422F91" modified="2023-11-08" version="1">MADYRPAAPKIQEQDLEGKLAIVTYDFSFISRLGFYMLSYEMSWSLKKESASPDYRLIHLIVSLSLSLTPKAVIFLKPTNIPPSSESFTFSSLQSQGATKGIGRAIALDLATRGCSLLGTYTSPSSAHNFDTLRTTVHGLYNTNTPSTPSSPLLKGLAADITNPASIAPLLSAVRSADSSFPQNKIDILVLNAAYNTRPKVGAATTADIQLSLTANLHWPILLVEELVRAHLFNPNARIVVLSSDRVRSPTPGSSIFNATKAGLESLARSWAVELPLSFPGTTVNAVSVGLTDTPGLRAFPAEAVEALKKERLGKVRVGGGGEGQGRRENGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM50</accession>
    <name>A0A9W9CM50_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mso1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005554</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM50"/>
    <dbReference type="OrthoDB" id="2683368at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028095">
        <property type="entry name" value="Mso1_N_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14475">
        <property type="entry name" value="Mso1_Sec1_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mso1 N-terminal" evidence="2">
        <location>
            <begin position="19"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="291" mass="30352" checksum="BE60FD0CB1225A5D" modified="2023-11-08" version="1">MSSYLNNLLTNTTSRYNNLRRQLLSDDADGDTEDDSHISRVLRAYYTEKGRPFPQWLPPDPKAPQPAPAQFASSSGRQQGQAAPIGRGGGLSDLWDSPQPQPLPQEPLSLRRAAGGRGGGARPLQGRMGNSLTPEPQIQGRPLPSQRAGSYQSTLGGRPQAEPSPPPSAGSGTSAQERLKARLWGSGRSSSPAQSAPSADSGSGMRNPYERPAPQQGGRAPYPDDRSNSYGSTGSGGGRQQSSASTSANSPWMGNDDPYAPQGGGGYGGGAPPQRGPQGGRVGLPSGPRMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM94</accession>
    <name>A0A9W9CM94_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">N-alpha-acetyltransferase, non-catalitic subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MAK10</name>
        <name evidence="7" type="ORF">N0V83_005449</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAK10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM94"/>
    <dbReference type="OrthoDB" id="269405at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057983">
        <property type="entry name" value="NAA35-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007244">
        <property type="entry name" value="Naa35_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057982">
        <property type="entry name" value="TPR_NAA35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373">
        <property type="entry name" value="GLUCOSE REPRESSIBLE PROTEIN MAK10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373:SF0">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 35, NATC AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04112">
        <property type="entry name" value="Mak10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25789">
        <property type="entry name" value="TPR_NAA35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAA35-like N-terminal" evidence="5">
        <location>
            <begin position="81"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="NAA35-like TPR repeats" evidence="6">
        <location>
            <begin position="374"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4369687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="803" mass="90424" checksum="D36E639E27DE4892" modified="2023-11-08" version="1">MADAPPTRHSTASAQDQDAIVTSNAPASSEAQESATTSAQAPTSANLPLRQSLRQTPGSVAPVIHDITEKFTKACSALEVGQLVKDDFFTLFESIGALEIMDPKMDSGFLQPGETLEDDYDCLTPLLPEELIGILDQLLCYEMAWHTGYPLSQTLFTSTYIDKLLWPETKILEQVQFYRGEIPDHKRPGPLLQVLRAYCLALIKGCDFVIAKVTGRDYFEEEDFCTQTYNRVLFVSIPMDVFLRELDAAMEVMEDRDLEINEALRTAILSRLEFRKYFLRAVDLDLPLDQMRYSWPPILDNLHTLKNTHQLGQVVPGAFSTKIQRRLASTVPPRPIVELEFKDAWDKLQQLCADCEEATRFIHLPQDPLEYQSFLWFFAARSPAPLAYARSYLGTLLFHPEVLESSVSFPLVDVKTLVFPASPVMDPTNWTLSPPRNVMLPKPPRLQFAMLMDEFVDRSGQPYLDLWVALGQNRCRLRRMLTHVITGWDMLQADASLVDTDISQAASELDIGDQILDFSLSTWVYHKKLWMIEKILLLGFEQDIYLPDEFSGMYLFLSLIATRRKELLTRIEAHHATRSAQLLASSRKTHTHLHDVYEVEAAAPYLAALLAEAEATASLSLALARFYILLLYCHLLPLPRRALLANEKLQYELRMKPFLALQPPEAPPFEDFKAHTQPYGDFDDPDPGFALDLRNPESDLWGEVERHLRGAKEAFAELKKMGAGACKAQGVEAAWGGMYREVGELRCVGDCGCGVRDAVGKREESGGGDIEGKKLGVRVEVPESGKGKRYSEGWIVVKILKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMR5</accession>
    <name>A0A9W9CMR5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein mS41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">FYV4</name>
        <name evidence="7" type="ORF">N0V83_005185</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMR5"/>
    <dbReference type="OrthoDB" id="18595at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039603">
        <property type="entry name" value="Ribosomal_mS41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019083">
        <property type="entry name" value="SAM_Ribosomal_mS41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28235">
        <property type="entry name" value="PROTEIN FYV4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28235:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09597">
        <property type="entry name" value="SAM_Ribosomal_mS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01238">
        <property type="entry name" value="IGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small ribosomal subunit protein mS41 SAM" evidence="6">
        <location>
            <begin position="4"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4370664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="196" mass="21422" checksum="02FAE1CD2E2FB133" modified="2023-11-08" version="1">MILRRPLLTTASSCTHIPSWDALFTLTSPQLKELGVEPARSRRYLLQWREKYRNGEYGIGGDCAHVNPEGVAELKVVEAPVMPNPQLGGTISPRSAAATATHDPGTRKFVVNVPVGDEAGSVEALPKVQGVIVKGAKTIKGSYVEPVKGSEGLRARIRLQEGIWEVKRGHKVDGGERRKAEVRAKRRAAENKEKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNA5</accession>
    <name>A0A9W9CNA5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNA5"/>
    <dbReference type="OrthoDB" id="73875at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="197" mass="21912" checksum="8C4DCFEEE58938AC" modified="2023-11-08" version="1">MRLGNTHIPGSILSPIDENEEEEEEEEEEEDEEICALPQKTTKTVGVTVTATSEKTVIVEPSTTSKAPAPAKTPDFSKDQTKCYDSGQWTRRARMVNAADTMCDSYLKGKTLMEKWNPGEVKSTFDRVDSEVTGVQIVAFVEANPGSGCEWKVDVNQCKTEFRKIIDECDTNGENRKQGGRLVGDCLTWRLDPNSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNH3</accession>
    <name>A0A9W9CNH3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PEBP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003249</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNH3"/>
    <dbReference type="OrthoDB" id="10251855at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00457">
        <property type="entry name" value="PEBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049556">
        <property type="entry name" value="PhiB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30289:SF1">
        <property type="entry name" value="PEBP (PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30289">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YBCL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4372958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="23436" checksum="FE57C44B9BBDAE19" modified="2023-11-08" version="1">MSLLKLLEYCIGCLLYRLRGYDHLCLFKTRAFAQQPKPSIPVTSPDCGPSGALLNKHYSKFGVGRFPTLTWENPNLNIKEYLILTEDPDAPLRKPNVHGIYLFIPPSVTSITNDDLELVSEGEDGVKSIKAGYRVGKNRRDVVYILPRPPLGHGPHRYFFEVVGLSEKLDQASLSKVPTKKEVEDAIVGKVVAWGMWEARFEHRWND</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNL9</accession>
    <name>A0A9W9CNL9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRELI/MSF1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNL9"/>
    <dbReference type="OrthoDB" id="341300at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037365">
        <property type="entry name" value="Slowmo/Ups"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11158">
        <property type="entry name" value="MSF1/PX19 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="1">
        <location>
            <begin position="2"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="284" mass="31578" checksum="152874AA4DDBDDAC" modified="2023-11-08" version="1">MVKFYSSNETYSYPFPAVTLAYFLRYPNPYSTHVLSTDTIARHYDPESQRLTTIRLHLKRSKLPSAVLKLLPRSILGASASGDTQSYILEKSVVDVKEGWMDTESRNLEWTGVLSVVERQMFKRPAPPAVPADEAKLWQGMEGAARRHGFINFGGMGAGAKVEDTGETTDVTSSVTLHSHIGETWKRKREAAREGTAALVDDEQPQKVGFLRSWGTAALQRNIEKIGLTRAQRSQPNAREGMKVVLERMREGGLVAVLDGMRQDREAILAGRPLALPPRGDNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP33</accession>
    <name>A0A9W9CP33_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pyridoxine biosynthesis protein</fullName>
            <ecNumber evidence="7">2.3.1.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PDX1</name>
        <name evidence="7" type="ORF">N0V83_002872</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.12" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP33"/>
    <dbReference type="OrthoDB" id="202158at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004742">
        <property type="term" value="F:dihydrolipoyllysine-residue acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000010">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="entry name" value="E3-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045257">
        <property type="entry name" value="E2/Pdx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151">
        <property type="entry name" value="DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151:SF82">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE COMPLEX PROTEIN X COMPONENT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="5">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="6">
        <location>
            <begin position="141"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="55"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="89"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4374131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="398" mass="42307" checksum="57D19EFFC6FCDD56" modified="2023-11-08" version="1">MPALSPTMTEGNIATWKLKEGDSFSAGDVLLEIETDKAQMDVEAQDDGILAKIIQGDGSKAVQVGTRIGVTAEPGDDLSSLEIPEEDSPAPKKAEASKEQPKEESKKESEPVPKEERTASPAQSDAPKKSSSGKATKQTYPLYPSVQHLLEINGLPKEEADKIPASGPNGRLLKGDVLAYVGKIQDSYPSELAQRFEKLAHLDLSNIKPAAKKESPKKPAAVAEAALAVEDLDIEVALPISLTAVVECQKRVKDSIGVFLPLSTFVARAAELANEDLPKSKTAKPSADELFNAVLGLDKVAGKQYSRGRFVPQMTVLPPTTLSSYRAPASKKSDVLDILSGKRSAHGSKASSVGGADSVVGPLNVFSVSVPKGDEKRGRVFLERVKSVLEVEPGRLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ13</accession>
    <name>A0A9W9CQ13_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-dependent RNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_002978</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RdRP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.48" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031380">
        <property type="term" value="C:nuclear RNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007855">
        <property type="entry name" value="RDRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057596">
        <property type="entry name" value="RDRP_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058751">
        <property type="entry name" value="RDRP_helical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079">
        <property type="entry name" value="RNA-DEPENDENT RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079:SF55">
        <property type="entry name" value="RNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05183">
        <property type="entry name" value="RdRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26252">
        <property type="entry name" value="RdRP_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0696">RNA-directed RNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RDRP helical" evidence="4">
        <location>
            <begin position="475"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="RDRP core" evidence="3">
        <location>
            <begin position="559"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="100"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF26252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4374237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1319" mass="149096" checksum="E336BAD5D58A2EFC" modified="2023-11-08" version="1">MDRGYNGYRRGRGSQTGRGDFRGGRGGDRTYSNSSGSSNSFNAQRPTLRASSPIQGLTTPPEQYARTPNRIDAPGSPVTRQSSFTLNGHNINETYTARLNTLNSPSPHSTPPLSARGRNQQRYQSGGQRLTYRPVMKDGDLHWAHRQEMKIKLSGLPKCCWTKDVYSSLSPYGTVVRIDLLPGSLDNSAYVTFQPPPPNALLNQRIRFGADPVKIDQVNSHVNTIASPVTPTKRYHEYNILSANSLDFGVRTADTTMIAMRTLESHGRIQLTLNLKRKELDIQFPLQVGNQTRKCRFRLPISLLSNIYKGSDPNSSQFTLIIPCNSPPQFFTQKNEGEKLVDGRNHTSFSDNERIWNDWDTWYRETSVVDNVVRRKLEISPVMNHSDEAIIDIGRWTDYRLSFDEATLRSAKFQEFSNALADHGVALKGLDYSVKDSTASPLWSLLQEEISGTHPHLESSSTASKFDDLLAGQVHLSFPIRYQLEACLSNGYLKEYNITKEFLEKLASMEPRRAIHILEKVCDKQNTYYDPMEIFDIRSKGNLEKKVPSYCILQRSAIITPTMIHVVSPIMETSNRITRKYAADADRFLRVKFSDEKTEGVLRNMPNDRAEAVFDRVRRAMTHGIVVAGRFYEFLAFGNSQFREHGAYFYAPTSSKSADDIRLSLGQFNHIKTVAKFGARLGQCFSTTRAMRVNVKVQEIPDVERNGYNFTDGVGKLSSFLAKMAAQELGLANPFDDPPSLFQFRLGGCKGVLALDPNIPGHEVHIRPSQHKFEAEFAGLEIIRSSALATPFFNRQIIMVLSNLGVPDHVFIRRQQDMVNDYEQAMTKEDIALRKLRKHIDMNQTTLTMAGMVLDGFMKARDPFIMSLLKLWRACTIKNLKEKARIAIEDGAFVLGCVDETSTLKGHLDDPQSRLDATRNEKLATLPEIFLQVDDTSRKGYYTIVEGVCILARNPSLHPGDLRVVRAVDVPALRHLKNVVVLPQTGDRDLANMCSGGDLDGDDYMVLWDEDLLPEIINVPPMDFTPDKPIETDKPITIADISAFFVTYMKNDSLGQIAHAHLAQADLNEDGVSDHICLNLANLHSQAVDYPKSGIPAVMDRDLRPHRWPHFMEKKHIAESRIYKSRKILGMLYDQVQLVDFKPQWENAFDERILNAFDFELDAQLLEKAAEIKISYDESLRRLMAKHGIRTEFEAWSVFVLAHNHESRDYKFAEEFGRTIGALKAQYKELCRTATEADGISVAEWSRLGPFVAAMYTVTAREMETALKECRATRIVGGQEVPARDMTPEYMPLISFPWLFPSELGKIALEKGKAIPKRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ65</accession>
    <name>A0A9W9CQ65_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sodium bile acid symporter family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003129</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ65"/>
    <dbReference type="OrthoDB" id="188035at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016833">
        <property type="entry name" value="Put_Na-Bile_cotransptr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18640:SF5">
        <property type="entry name" value="SODIUM_BILE ACID COTRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18640">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 10 MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13593">
        <property type="entry name" value="SBF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="265"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="337"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="370"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="404"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="447" mass="50156" checksum="94334024429B0E69" modified="2023-11-08" version="1">MKHPQSGERSSPAASSKENVEHMGIEQATMEAKRMESIPIGDPGKQLDAPLQAKGTLPSDEQEQEQEHEQDIGQPHKPDKTSKRAKTLKVLKWFIQDQWFLVAMAAIILISSQVQVPASQQHIKRTVITYLSVSVIFFINGCTLETNVLLQNYMRWKLHAFVQLQCYLVCSAATFAIVSLCATNPSFMDPWLLIGFLFVGSAPTTMSSNVVMTRQAHGNAALTVVQSVIGQFLCPFLTPIILQMYLGAGAWYSKVLVHGDGYGEIYRRVFMQLGLSLFIPMLAGQIVQKLFPKQTKKVFFDWKLMRLSSISLLTLVWQTFDQAFQSGAFKQVKSSNLIFIVFISIALYFIWLAICFAASTFWLPKKDVIACCYCCPAKALAMVVPLSSVMYINISPVEQSKMQIPAIIFQAFQVGIGGILTICFRRWIRPEEQKEEAEKNVEAGQDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQZ4</accession>
    <name>A0A9W9CQZ4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Short chain dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001171</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQZ4"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43490">
        <property type="entry name" value="(+)-NEOMENTHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43490:SF99">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4375893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="247" mass="26609" checksum="1A04C2F6FAE7D297" modified="2023-11-08" version="1">MSSTSKPVALITGANRGIGFELARTLARDHNFQVIIGSRSDSSGLEAFSKLRAEGLFSVTGVTLDVSSDDSIVHAAKSVEQNFGRLDLLINNAGIMLPDSQDSPIATKRKAFQSTFNTNVTGAAVVTETFIPLLSKSTLPRILFLGSTLGSITGRLDENYIWDSMDSISYRCSKAALSMLAAQYARRYGEKGWKVNIVCPGYVKTGMVNFMGTITTEEAMPHLVKMCTLGENGESGTFSDAEGTVLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR05</accession>
    <name>A0A9W9CR05_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001187</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR05"/>
    <dbReference type="OrthoDB" id="5047692at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4375909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="319" mass="35845" checksum="6EBF4AB8F94F4F17" modified="2023-11-08" version="1">MVATTSERLQSPFLKAYVNALRSHLAPESDLSAAGKAIFVSTNLQRGIPIKTFAEYINVRLYSIADSVQSGGPVFSEAEASYVDFLKEYVESVQESSKPDYAKVKIAEEQYRRAKQEASMQLELATYRFRTVADLDKTAVFEDFLQKHGIDYRDACEERDVARAEMKALKTTSTADVKKARGMLDIALNSVNVVPGISMACATEDVDDRALRTNIEKPSSVNFRPHHTLEGFDFTGHIWRDSGLVDQLKQSRLDVDLRHGFDGVTWAELGFPDISDRVSGFASPMPDEQKTAIFNVLEKWDMHIQYTGLHAFDITRGDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR60</accession>
    <name>A0A9W9CR60_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001219</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR60"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4375940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="177" mass="20234" checksum="53CD853BE816E4B6" modified="2023-11-08" version="1">MLVPVAINLLGSRPYPFEEHRVHEGTREAGKLYWQWDAYTEPELQELFLNMPLLNISDAVLIIPPIVYDRPSAAKDSNANAFLPRAYTRTWLINPALQPDDLVAPYYNGPSTGLKDSIRWPVKGRAGYAIPTLEWYFGGEEEDPVKPHYTNEKFVISLDLKLFEIYLIREEVAAELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRC6</accession>
    <name>A0A9W9CRC6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000685</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRC6"/>
    <dbReference type="OrthoDB" id="7289984at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052184">
        <property type="entry name" value="SDR_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45458:SF3">
        <property type="entry name" value="CHAIN DEHYDROGENASE (ATSC), PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45458">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE SDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4377855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="274" mass="29055" checksum="75346F74898339BF" modified="2023-11-08" version="1">MASYLITGASRGIGFAFLENISSDPANTVVGLVRNKTDTETKVAALKRTNIHIVQGDLNNYTSLKSAVEATARITGGSLDYLIANAGLLSTPRSKPFATLGELGKDPATLETELTEMFQTNVIGNVHVLNLFMPLILKGNVKKVVALSTGLADLEITRAHNLHENAPYAISKAALVRKNMAMGKFSAQYAKDGVLFISICPGVVDTGNLADMPPEDGPGLMAMMEKFQGYAPHFTGPAIPNDAAKDVLNVAQKASVPNGDGGSYVSHLGNKQWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRH5</accession>
    <name>A0A9W9CRH5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Small ribosomal subunit biogenesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ENP2</name>
        <name evidence="10" type="ORF">N0V83_000850</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat NOL10/ENP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRH5"/>
    <dbReference type="OrthoDB" id="273340at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056551">
        <property type="entry name" value="Beta-prop_NOL10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040382">
        <property type="entry name" value="NOL10/Enp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056550">
        <property type="entry name" value="NOL10_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012580">
        <property type="entry name" value="NUC153"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14927">
        <property type="entry name" value="NUCLEOLAR PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14927:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23098">
        <property type="entry name" value="Beta-prop_NOL10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23097">
        <property type="entry name" value="NOL10_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08159">
        <property type="entry name" value="NUC153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Nucleolar protein 10-like N-terminal" evidence="9">
        <location>
            <begin position="15"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleolar protein 10-like second" evidence="8">
        <location>
            <begin position="409"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="NUC153" evidence="7">
        <location>
            <begin position="550"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="567"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="651"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="693"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4378020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="726" mass="80667" checksum="9674C8776B99B2BE" modified="2023-11-08" version="1">MKISNSAVPVYTISGSEARPLPDWLVRRRKRSLKNDPEFANRVELLQDFGFDEASQCVRVSEDGDWVMSTGTYKPQIHVHYLPHLSLSFARHTNSLNQTFRLLSSDYTKSVHLQSDRVIEFHTNGGLHYSSRIPRYGRDLIYDRRSAECLVPSVGVNAEGLGEVYRLNLEVGRFMRSYEVDVGGDDLTTVGSGSLQGGIDAGAVNTGAIAEDSHNLLAFGTSLGTVEFWDSRSRNRVGILSSPQDAFEGRSEITALTFPRSGLELAVGDSNGLVHLYDLRSPKPLLKKDQGYGFPIKNIIYLNSSTTSRAQTAEPKILTADKRIIKIWDPRDGASWTSVEPAVDLNHVEWVKDTGMLLTANEGKQQHSFFIPQLGPAPKWCAFLDNIVEEMAEDPNDPNAFGRGSAGEVYDNFKFLTMPQLKQLNLDHLVGTTSLLRPYMHGYFVAQKLYEEARLISNPDLWQEQRAKSIAEKINKERESRIRGNKKVAVKVNRRLAEKMLNKEEEKERRRAKRVLAKGGDDDMLVDTDAAPAVGAIEDPSKASSGVLADPRFARLFEDEDFEVDERSHEFQALNPSTKIPKGLTAAEQEDLESHKGSSDSDDSDDSEREQTKPSARKQPRQKEDTSRISSSNYRKAGHRDQRGGPQMVVSSSNARKSSSGQAKRDATFGSRVSKLKDKRPSASGANTTTVVGEREITFAPERKQRKRSGGQGGGEGPRWTLGLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRP6</accession>
    <name>A0A9W9CRP6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial genome maintenance protein MGM101</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_000974</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
            <location evidence="1">Mitochondrion nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MGM101 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRP6"/>
    <dbReference type="OrthoDB" id="17164at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000262">
        <property type="term" value="C:mitochondrial chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009446">
        <property type="entry name" value="Mgm101"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31404">
        <property type="entry name" value="MITOCHONDRIAL GENOME MAINTENANCE PROTEIN MGM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31404:SF0">
        <property type="entry name" value="MITOCHONDRIAL GENOME MAINTENANCE PROTEIN MGM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06420">
        <property type="entry name" value="Mgm101p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1135">Mitochondrion nucleoid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="48"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="75"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4378141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="299" mass="32582" checksum="EE68464FB21A6DCB" modified="2023-11-08" version="1">MATVSARKGFSAFLSASTSRAAPIAHRPLHRTFSASTLRAAYATAPKAAATSTAAKPTSTTSTASKPSTSTTTTQQRTVTPKSPQRSPIPTNRTAHLDTLTSAARSQRSLTEGLSDAPPPLEGVPAIDWTRSYHGLGSISFTGEQAEKLLAPIEAADVEVKPDGILYLPEIKYRRILNNAFGPGGGDWRRGGRVSLRASWLRGSMGWLFRGVSIARGEQQYFDPDGIPTATEGCKSNALMRCCKDLGIASELWDPRFIREFMGKMTKEVWVEHVSTKKKRKVVIRKDDVVKYPFKETKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRY5</accession>
    <name>A0A9W9CRY5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Glycerophosphoinositol permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">GIT2</name>
        <name evidence="10" type="ORF">N0V83_000382</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRY5"/>
    <dbReference type="OrthoDB" id="2261376at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000140">
        <property type="entry name" value="Putative MFS phospholipid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="388"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="426"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="464"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="37"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="511"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="514"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4377557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="535" mass="57517" checksum="36A812417BDDCE2F" modified="2023-11-08" version="1">MASAVQSQPHDLASKDDIERRGSVARTEKGRWERLWPVIACGAGLFSDGYLNNVRFHEAPNQTNATSPPPKAPPLAIAILSGIIGPVNTMLKTIYKDEYTNSSAQANVSSITFAGTVVGMLFFGYTSDHFSRKWSLFTSTIIIILFATLGTASYGAGGNPNGLLVALVVYRFFLGIGIGGEYPAGSVGCAESTGELREGTRNKWFILFTNVQIDLAFFISAIVATVVVLITGENHLRAAWRICLGIGILPPLSLLYLRFKLQEPEAFKRESLAKAQTPWLLIIKFYWFRLSIVSIIWFIYDFSAYSFGIYATSILANLLGPTAPLWKSLAWNILINFFYMPGCLAGAFVADMPSMGPKKTLCIGVALQGIIGFIMAGCYPYLNKPENVAGFVVVYGIFLALGEFGPGDNIGLVASKTCATGIRGQYYGIAAAVGKIGAFAGSYALDSLQKAAGDDEVAAGRNPFFVASTLAFVAAGLVLLLPHIAQDTIDQEDVNFRAYLTANGYDTSKMGLDSASSTTTETEVNVPAETEKSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXB1</accession>
    <name>A0A9W8XXB1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Pre-mRNA-processing protein 45</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PRP45</name>
        <name evidence="6" type="ORF">N0V83_011014</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SNW family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXB1"/>
    <dbReference type="OrthoDB" id="666364at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017862">
        <property type="entry name" value="SKI-int_prot_SKIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004015">
        <property type="entry name" value="SKI-int_prot_SKIP_SNW-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12096">
        <property type="entry name" value="NUCLEAR PROTEIN SKIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02731">
        <property type="entry name" value="SKIP_SNW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="SKI-interacting protein SKIP SNW" evidence="5">
        <location>
            <begin position="184"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="163"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="318"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="521"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="225"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="318"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="359"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="523"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4362072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="556" mass="63015" checksum="30962BC91570C7A7" modified="2023-11-08" version="1">MSVAALHKSLPKPQYTGEEEELTRSTRVLTSEQFETQVARRQNGPPAYGSRHSWRPRTAEDFGDGGAFPEVHVAQYPLDMGRKGTPSSSNALVRRVDGEGKTKYDEIARRGHNDSRIVQASFKDLIPLRQQANAGELDLARPSQEVVQATKEKTEQALMALVSGQTAAQKPKNVQGRKNDEPTFVRYTPTAQMGEAQGKTRIFKIQQRQIDPMEPPKFKHKRIPRGPPSPPPPVLHSPPRKLTAEDQEMWKIPPPISNWKNPKGYTVPLDKRLAADGRGLQDITINDKFAQFGEALQAADRHAREEVKQRAIMQQRLAEKEKLQKEEHLRNLAKQAREERSNTSRRRSQSDSETDSEEEAVRKRMEARKERREDFQREFRQSKMGTERKIQMLAREQNRDISEKVALGLAKPTQSGESMYDSRLFNQSSGFNAGFNEDNHYDKPLFAAQDAISSIYRPTVGQDDGEDAEETYDRLTKSSKFEVLGKAKAGFKGADIQEQRDGPVQFEKDTDDPFNINQMISEVRGESGEKTGEKRYGLQESEGRAAKRARVEDDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYH1</accession>
    <name>A0A9W8XYH1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010394</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYH1"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="56"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="246" mass="26260" checksum="A6624B638144128C" modified="2023-11-08" version="1">MAYNPLANAPKQALALLEGQGMVAVYEATGGKVTSDFAAHNLTLIPPIPSGSIIHDNACGAGTVSRLILSHSSPAAPDVKIHATDIDPVFLSTLESDVAKNSWPVEVSNQKAEALSFPDNTFTHSITNIAIFMTASAGLDAAKEIHRTLQPGGVAIVNCWEALVWLFPIKLVAESTRGGKPMPAPPISWNDGVQIQKIMAEAGFKKENMRVDRAEVWAKTSDLRGGPRRVGRFWAGWAGWAGGRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYY0</accession>
    <name>A0A9W8XYY0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cupin type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_010065</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYY0"/>
    <dbReference type="OrthoDB" id="2205143at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02216">
        <property type="entry name" value="cupin_GDO-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047183">
        <property type="entry name" value="GDO-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41517">
        <property type="entry name" value="1,2-DIOXYGENASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41517:SF1">
        <property type="entry name" value="CUPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="4">
        <location>
            <begin position="105"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4362948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="239" mass="27083" checksum="FF3BED040E9D885D" modified="2023-11-08" version="1">MSSTTTTVTRNGDSSHIDPQQATDQLLRSAEQQHTKPLWTQMARLNPPLPNPKCVPYVWEYEKIRPSLLRAGELITEKQAERRVLMLVNPKREAPYTTDTIYAGLQLVMPNETAPAHRHTAFACRFIIEGNGGFTAVHGRRIQMQRGDVILTPTWNWHDHGKDGSGPMIWLDGLDLPNFVHYPVHFVEHYADPRYPAKDVDTSASPIVFPWSRMKAQLDESGDENISKPYLRADGSESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ42</accession>
    <name>A0A9W8XZ42_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cerato-platanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_009852</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cerato-platanin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ42"/>
    <dbReference type="OrthoDB" id="4898945at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22778">
        <property type="entry name" value="DPBB_CEPL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010829">
        <property type="entry name" value="Cerato-platanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07249">
        <property type="entry name" value="Cerato-platanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040901166" description="Cerato-platanin" evidence="4">
        <location>
            <begin position="21"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4363556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="14522" checksum="81EC0C6BC398827B" modified="2023-11-08" version="1">MKFSSAISAAALGFASLTSAISVSYDTGYDDASRSMNAVSCSDGPNGLETKHGWQTQGAVAGFPHVGGYQGIAGWGSDQCGTCYGITYNGKTVYVLAIDHTATGFNLAKQAMDELTNGQAEFLGRIEANYAQVDVSNCGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZK6</accession>
    <name>A0A9W8XZK6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dihydroneopterin aldolase</fullName>
            <ecNumber evidence="4">4.1.2.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">7,8-dihydroneopterin aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_009406</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde</text>
            <dbReference type="Rhea" id="RHEA:10540"/>
            <dbReference type="ChEBI" id="CHEBI:17001"/>
            <dbReference type="ChEBI" id="CHEBI:17071"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="EC" id="4.1.2.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DHNA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.25" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZK6"/>
    <dbReference type="OrthoDB" id="5425486at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
        <property type="term" value="F:dihydroneopterin aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006156">
        <property type="entry name" value="Dihydroneopterin_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006157">
        <property type="entry name" value="FolB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42844">
        <property type="entry name" value="DIHYDRONEOPTERIN ALDOLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42844:SF1">
        <property type="entry name" value="DIHYDRONEOPTERIN ALDOLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02152">
        <property type="entry name" value="FolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00905">
        <property type="entry name" value="FolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroneopterin aldolase/epimerase" evidence="8">
        <location>
            <begin position="152"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4363953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="283" mass="31689" checksum="3B611466C0791C9B" modified="2023-11-08" version="1">MAKLVQQFQWDAQVAQSGHYDKINVQNLEVVVNAGKDVWGRPKKQRALISVTLTLGKQFTSASSTDSVDDSTIHYGTLSKAIQAQFRDEDRAWLSTVALSATIGECVQDMAGATEIYAIETDVCYLKGSMFGDGAGYVNSTIGNSNLHSKVLYLRNLRIPCLIGVNSNERLQKQPVVANVWIDQVPDSRVDDYAELETLLFKVGMLRILDQALTDVRQLISESSYQTIESLLAWLIEELRQKFFTREEDHDAWIRLRIAKPLAVPFADAPAVEITRPVRANQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZT6</accession>
    <name>A0A9W8XZT6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRISE-like Rossmann-fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZT6"/>
    <dbReference type="OrthoDB" id="1731983at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08948">
        <property type="entry name" value="5beta-POR_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055222">
        <property type="entry name" value="PRISE-like_Rossmann-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32487">
        <property type="entry name" value="3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32487:SF8">
        <property type="entry name" value="NAD-DEPENDENT EPIMERASE_DEHYDRATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22917">
        <property type="entry name" value="PRISE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRISE-like Rossmann-fold" evidence="1">
        <location>
            <begin position="5"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="391" mass="44299" checksum="C0077EE9870E84B9" modified="2023-11-08" version="1">MSKSALVFGASGVTGWSFINEILSDYPAKNTWKRAHALTNRPLSQEQSQWPNDPRLNIVSGIDLLANSQESLEKELKEKIPDIDEVTHVYYLAYKAGTDVQKELEEAVDMWKKTVIAVDRLCPKLEFVVLQTGAKMYGCHLLATVPEYSAPYISPPHKENQPRIKGQFGDMLFYHPQLDFIADYAKDKKWSWCDTRPDIIIGFVPNQNFYSLGTSIGIYLSLWKEVHGAGSECPFPGTSKSWNALSNDSSSDMIARQTLHLSLNPSTEKGGGYNVADEQNPSTWSAKWPVLCDYFGLKSTGPSENPPEIRSFIKENIGKWKEVEKRDGLQEGHAHNDKVFAGFEYFLMTQFDFDRQYDMSKMYGAGFTEERSTKEAWGGVFDRMRKAKVIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y072</accession>
    <name>A0A9W8Y072_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Small nuclear ribonucleoprotein E</fullName>
            <shortName evidence="11">snRNP-E</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Sm protein E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_010079</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in pre-mRNA splicing. Binds and is required for the stability of snRNA U1, U2, U4 and U5 which contain a highly conserved structural motif called the Sm binding site. Involved in cap modification.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y072"/>
    <dbReference type="OrthoDB" id="25620at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01718">
        <property type="entry name" value="Sm_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000031">
        <property type="entry name" value="Small nuclear ribonucleoprotein E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027078">
        <property type="entry name" value="snRNP-E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11193">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8 11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="12">
        <location>
            <begin position="17"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4362962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="94" mass="10535" checksum="E337998602126B9D" modified="2023-11-08" version="1">MTGRGGRGGGKVLLPPINFIFKLLQSRATICVWLYENLGLRIEGKLRGFDEFMNLVIDDAVEITLAHGEVPEQRRKIGQMLLKGDNISLIQQVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0N2</accession>
    <name>A0A9W8Y0N2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Coronin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CRN1</name>
        <name evidence="12" type="ORF">N0V83_009702</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Binds to F-actin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the WD repeat coronin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0N2"/>
    <dbReference type="OrthoDB" id="1850764at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000197">
        <property type="entry name" value="Coronin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015505">
        <property type="entry name" value="Coronin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015048">
        <property type="entry name" value="DUF1899"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856:SF0">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08953">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16300">
        <property type="entry name" value="WD40_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM01166">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01167">
        <property type="entry name" value="DUF1900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="DUF1899" evidence="11">
        <location>
            <begin position="4"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="77"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="133"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="175"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="406"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="585"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="413"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="430"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="463"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="507"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="587"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU280818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4363409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="618" mass="67099" checksum="8DF19EF8AB490BDE" modified="2023-11-08" version="1">MAGRFVRASKYRHVFGKGTKKEQCYDNLRISKNAWDTNLIKANPEYVSVNWEASGGGAFAVIPINERGRAPDQLPLFRGHTAAVLDTDWSPFNDSLISSASDDGKVFIWKVPEGFALHTDAEEPADVAPVAKLSGHMRKVGHVLFNTAAENVLASSSGDYTVKLWDVEAGAAKLTLKHNDIVQSLSWSPDGSLLVTTSRDKKLRVWDVRQEKPAQEVPGHPGAKNSRAVWMGETDRIATTGFSRMSDRQLGLWDPRNPKEPIGGFQILDSISGVCMPFWDDGTQCLYLAGKGDGNIRYYEYENDKFEYLSEYKSPDPQRGIAFLPKRGINLHENEVLRCFKTVNDSYIEPISFIVPRRSEMFQSDIYPPTTGLKPGTTASEWFGGKTAVPPKISLESVYDGEAPKEVASDYKAPQPAAAQPSPVKAEPPKAAPEPAPAPIASRGPPPSMKDNKDSLAAGASKFADKDGDSDNEKRHAYTAARQEEKTKGPVISKEPEPAKPIPAPTAAPTQAAKPTPAPAAAEPTPTPSAATHATSSAPAPASSGPAAVLRDTLGDIKSQLEHQNKVMSDQSDQIALLLREVGSLKTKLSGTSQQGPSDREKDERIRQLELELEEARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0S4</accession>
    <name>A0A9W8Y0S4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009031</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0S4"/>
    <dbReference type="OrthoDB" id="2240312at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000500">
        <property type="term" value="C:RNA polymerase I upstream activating factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000182">
        <property type="term" value="F:rDNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001181">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039601">
        <property type="entry name" value="Rrn5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28079">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28079:SF1">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="258"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="479"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="587"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="667"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="483"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="519"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="707"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="717"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="734"/>
            <end position="745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="759" mass="85369" checksum="B28E77EA1C334496" modified="2023-11-08" version="1">MSSESDYQTDHAPQESSEESSKPPTRPSSRRQSRTPNTPRKRPRVESVDPSRIRKYYLEGKYNDAYRVLFNEDVSLAAVRFNADSGSNQHYITQFGSSVWSSKEQSTLFAALERLGRDDIPGISSAIGTKSIPETQEFMLLLQDAATRQGYAKVTLRDIPAAIEVGNECCEQLDIAGEALAWYQETFEASQEQERFGDYWLITPAIADKIEDTVAGRSRAVSSTPASEPEPPRRGGKVVVGYVAMPAMETTLTFLRRSCTSCKQFKQKCDRGTPCGNCVRRKIGACVYKEKPTRSEKENLQGSTTLASEAEEIVSARVPARPNRNLLEAIPEAHLLQPAVMLSLSKNLFMNRSPTIPSPWPHWFEYTSELAEEPSIYRSAFNDFHTLAVSVTKRLAQTALIQATSRLRSQRRRFKKGVLPLVKKRDVLAAIDILGMKRNGKERWRTVARRCSVRVHEGRKDRRKEVSWDEVERIMKYAEASTEPLTTDAETSGNDTEAFNSRAARSGTPLPMEQLAISDSDEDIGTDDEYNESMAESDGSVLDEGRPPFTYSATQPRDPFRQYTGAPPASALHERKFDLGTLEQFDQEASRQEEQTLWEMLELEPPIQQEEMKNDDSDDEESLEDSGNLITDPDGWRTWSEYRAEWEEFRKAVPAAKFYVSEKPDSSITFARVKPSQGDSRRDTQPTSEVIELQARSTRAYAALQERTSEPNHQPEDSEEAWSEDAESDASADVPTQSIEDSTQAVDMAPSGSEMDWSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0X7</accession>
    <name>A0A9W8Y0X7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009620</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0X7"/>
    <dbReference type="OrthoDB" id="1933717at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF4">
        <property type="entry name" value="CHAIN DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G08710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="25514" checksum="02A39BAB942846D7" modified="2023-11-08" version="1">MSTASLPTHYGMTLTPTIHSTIPSSLDPTLTSLPRPFIAVVTGAGKGLGYHISLAFARAGCSGISISSRTMGDLEKLEEEIRKVRGKEVEVLKSVCDVTSDDSVRKLEEEVRAKWGRVDVVVANAGVISNYVTRSTGQGDGEGQEESNLPIGILEDSDWSRVLDINLHGTWRLSRAFMPLLLDNSGGKGGPKTLICSTSLASHSVNSQMTPIAYNVSKIACNRLIEHVANDHGGKGVQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1D8</accession>
    <name>A0A9W8Y1D8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_008951</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1D8"/>
    <dbReference type="OrthoDB" id="3790317at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF8">
        <property type="entry name" value="DNA-BINDING PROTEIN SMUBP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="626"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="93"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="608"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1186"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="93"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="616"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1201"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1214"/>
            <end position="1230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4365330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1257" mass="141391" checksum="F336ED54633F020F" modified="2023-11-08" version="1">MVATVEQVSVEWNALDAQMCDAITIQHPDMYPDMIPMDDRNGHSYAPIPTSSLFDLRASDIKRGILPETLNKGAKAMTVMIAGDYHAGKHMETSVELATERDADKVQKDQNQAQADPKDKGKGKAPASAPADVSQKMHVDQPTIPSNEVVKAYFHLDTQTPHIELLALDATTGRTISCRLFAHDINWDPEVAGRVGFRVEKYQPDPTVDRTAPKDEPENESKQLIEKMSQSFQQTCLIKVTIFLNSARIFRHHWEGSVGTFRHQWEGITDSDIAAVRADPDFTLLRDNFITALEKQPVIELYFTSKSGEGYTAKWEKKFGNYMRDLLTLAKYYGNFWFYRWQCQDAGLNANAILQPEFPNVDWIVPRWLVTDWNLTKTTTPDGTITYSEPRPYLWTPLEFPEYGYNDPNEAAFLLKMGVHEGRSRQKRDLSEVVQSDGSKWFKGRFRSLDKKNRSYAVEVFLGLDPQLMQDGLRMPSAGTRIHMEVDHINAEQPSKQNTIALDGVVVYDALQTTASFIVVVNVYGPPLDIADDDNAYHLFISYIMDELPHQRMMNGIAQLQLSGKFSGPDSRRTVLGCKVPAQGTNVLKTKTDEAACRLFEAAMAEVQPPSDERQTSGSLQTMNSDSGNVVIVGPPGTGKTATIMKIGHAHATLGRRVMYVAPLNANVHTLVDKFIQINTALPFARQYQEHEWVYFTGGYVGIEKAEKLRQDQLAGEDALNHANEKLFGYLQDAKERSHVPRYEQTLGYKLRHRIDAWASDECIDTEQADLHTQARSFLEAKEELPSLKDKDQRTQIKKRIRELEYNLSLAFLKGVKFCFCTLSTSGHQLILESGDWDLLIIDEAAHETRAGISVALGALAGRVKLIVWAGDHHQGEGVIVGAHSNVGYKLLSRNVFRSMAETDKKDSATPCEVTLLNICYRMDNRLIRWSSAWCYNNQIISHRRVGSTDMGLRNMLQAYWRQRFPDNFGRHWSDLGLDVTDIGVATEFMKGTTTKLNREEAKMIARTVLDMLTMAHPEVSPNQQLRRIEAKDIGIIANYSGQIMEIRRAIRERARELSVEQEDLATLSFATTAQAQGREFSIMFYSMVIATGEMRLPKESGLPIGFVSKINNLNVSITRCKIARYTVGALQLFAQANRDHHSVTRNRRNLGFFDFVNRLVESRSIVTLDESNRWFESGEKPVTRGGFAQRLLRPNSSDQKPAEKKGEPAEKPKRTRRGGLGGKRKKAKRAAAAAEAAEAEAEFSNRPPSPSGDAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1J8</accession>
    <name>A0A9W8Y1J8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 12">Ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="2 12">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">RNR1</name>
        <name evidence="15" type="ORF">N0V83_008686</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 12">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the ribonucleoside diphosphate reductase large chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="2 12"/>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1J8"/>
    <dbReference type="OrthoDB" id="3000483at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005971">
        <property type="term" value="C:ribonucleoside-diphosphate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01679">
        <property type="entry name" value="RNR_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.70.20:FF:000001">
        <property type="entry name" value="Ribonucleoside-diphosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013346">
        <property type="entry name" value="NrdE_NrdA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039718">
        <property type="entry name" value="Rrm1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02506">
        <property type="entry name" value="NrdE_NrdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573:SF6">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="R1 subunit of ribonucleotide reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00089">
        <property type="entry name" value="RIBORED_LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 12 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="14">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="771"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="857"/>
            <end position="882"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4365069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="904" mass="100887" checksum="B1ADDB1BF9410FFD" modified="2023-11-08" version="1">MFVFKRDGRKERVQFDKITARVSRLCYGLDPDHVDPAAITMKVISGVYQGVTTIQLDDLAAETAAYLTTTHPDYAILAARIAVSNLHKQTKKQFSAVVADLYNYVNPKNGKSQPMVSDSFYKTVQEHAEELNSAIVYDRDFNYQFFGFKTLERSCLLRINGKVAERPQHLLMRVAVGIHGEDIEKAIETYDYMSQKYFTHASPTLFNAGTPSPQMSSCFLIDMKEDSIDGIYDTLKTCALISKTAGGIGLNVHRIRATGSYIGGTNGTSNGLIPMLRVYNNTARYVDQGGNKRPGAFAIYLEPWHADVFGFLDLRKNHGKEEVRARDLFYALWIPDLFMKRVQENGEWTLMCPNECPGLADVYGDEFEELYHRYEKEGRGRETVRAQKLWYAILESQTETGNPFMLYKDACNRKSNQKNLGTIRNSNLCTEIVEYTAPDEVAVCNLASLALPSFVDYKRGVFDFQKLHEVTQVVTRNLNKIIEVNHYPVEEARRSNFRHRPIGMGVQGLADAFLALRLPFESPEAKKLNIQIFETIYHAALTSSCDIAKELGPYQTYPGSPVSQGILQYDMWNVTPTDLWDWASLKEKIAQHGVRNSLLVAPMPTASTSQILGNNECFEPYTSNIYSRRVLAGEFQVVNPWLLKDLVDMGLWSENMKNRIIADGGSIARIPNIPEETKTLYKTVWEISQRTIIQMAADRGAFIDQSQSLNIHMKEPTMGKITSMHFAGWKLGLKTGMYYLRTMAASAPIQFTVDQEALKVADTNIARALPKARKQPGFRESPVTASQIPPSAPQPMYATKSPNGALESNGVPTPVATPPPASEIKNTPTENALATKPFKADVEEGDSPKVLASDPASKPDEEVLAKDKQGEDGEESEARDGDIYADAVLACSIENPESCVMCSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1Q0</accession>
    <name>A0A9W8Y1Q0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009589</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NmrA-type oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1Q0"/>
    <dbReference type="OrthoDB" id="3358371at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05251">
        <property type="entry name" value="NmrA_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051164">
        <property type="entry name" value="NmrA-like_oxidored"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42748">
        <property type="entry name" value="NITROGEN METABOLITE REPRESSION PROTEIN NMRA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42748:SF31">
        <property type="entry name" value="NMRA-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="3">
        <location>
            <begin position="1"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="302" mass="33364" checksum="21D7A2DC6E4F1603" modified="2023-11-08" version="1">MSKLITVFGATGNQGGSVVKHILADAVLSKEFKIRGITRDTSKPAAQALTSKGVEMVSADLNSKDSLTSALDGSHTVFLVTNYWETANRDIEVTQGKNVADIAKQLNVQHLIFSTLLNVTKESGGRLSHVPHFDGKADVEEYIREIGVPATFYLPAYFMSNFEQFIKPGEDGVLTVALPVSEGAKFPLVDIKEDTGKFVKAIIKNRSTLLGAHILGAENYYTSTQVLDQITETTGKKTKFVQVSEEVYKSFLPEFMAQEMLENHLFIEKPGYYAGETLDKSHGILEDKLVSWKEFVEKSKAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1Q7</accession>
    <name>A0A9W8Y1Q7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MAPK-activated protein kinase Srk1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">srk1_2</name>
        <name evidence="3" type="ORF">N0V83_009640</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1Q7"/>
    <dbReference type="OrthoDB" id="1738954at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="269"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="339" mass="37629" checksum="A221EB27AF894A70" modified="2023-11-08" version="1">MKGKGAGGIGLIKVADFGLSKVIWDSQTMTPCGTVGYTAPEIVKDERYSKSVDMWALGCVLYTLLCGFPPFYDESIQTLTEKVARGQYTFLSPWWDDISKPAQDLVSHLLTVDPEKRYDINQFLNHPWIREADEPTYSAFDAPPLATPAARKNDRLQPDFSYLESPGARRQDFRSPGAVNLREVFDVGYAVHRQEEEGKRKKQFKQGYRGANAISSLNALDENEDDDEYSAESVPYESSQQHPPAKLPKSQGADVSSMEQKMRNTTLSAAAQARQQQTPRQQERGYGQHSPAVAAAAKRQVKNKGAFELSLDSATLLGRRAKKQPEPSGLREHITAGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1S7</accession>
    <name>A0A9W8Y1S7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Eukaryotic translation initiation factor eIF-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SUI1_2</name>
        <name evidence="7" type="ORF">N0V83_008329</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Additional factor that functions in concert with eIF-2 and the initiator tRNA in directing the ribosome to the proper start site of translation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SUI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1S7"/>
    <dbReference type="OrthoDB" id="10248435at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11566">
        <property type="entry name" value="eIF1_SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000005">
        <property type="entry name" value="Sui1 translation initiation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005874">
        <property type="entry name" value="SUI1_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01160">
        <property type="entry name" value="SUI1_MOF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10388">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004499">
        <property type="entry name" value="SUI1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="SUI1" evidence="6">
        <location>
            <begin position="32"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4365709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="117" mass="13140" checksum="A5C6D8C8F8FE9567" modified="2023-11-08" version="1">MTTDIQNLKSFDPFAEADDTGGQTQTSQQNYIHIRIQQRNGRKTLTTVQGLPKKFDQKKILKVIKKKFACNGTVVADSEMGEVIQLQGDQRKDVQDFLTDKKEGLGLDGKTIKVHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y261</accession>
    <name>A0A9W8Y261_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ABC-type transporter tr06</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TR06</name>
        <name evidence="14" type="ORF">N0V83_009227</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000057">
        <property type="entry name" value="ABC multidrug transporter SitT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000913">
        <property type="entry name" value="ABC multidrug transporter SitT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF11">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="310"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="786"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="830"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="911"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="935"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1017"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1048"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="81"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="407"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="791"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1111"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="469"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="740"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="477"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="505"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="750"/>
            <end position="763"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4364631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1353" mass="148000" checksum="885740D9F49213A7" modified="2023-11-08" version="1">MTSSDKVEPPPPPPAATAGAETAALNAQVLTAGNTENDRVADLTEQMTDDAGKTKKPSQAGLGNYFRVFTYGTKFDFVLIALCCLTSIGSGIAMPLMNIVFGQLVGNFTNYFILPTTVTKHEFMAEINRLALFIVYLFIGKLVMSYISMLTVRISGLRISAALRLAYLRALFAQPISVIDTISPGKVSTRITTSSNTVQLAISQHFAMLFQSLAFTIGLYVVAFIKGWLLTLVASASLPFILIVYGALIPPFIRIHKTTEKTLEDASAIAFEMFSSVRIVVAFGAEAKLARQHKQLLDKATLNERKAAPLMGLMMSPSMLAMYGTFAITFWFGIKQYTEGKIPNVGAITVVLFSVMMAVMNIGRLASPIIAIAKASSAAHELFATIDAPAPDMSGVKEPEISADANITFENVAFSYPSRPNVEILKGLDLSIGAGKTTAIVGPSGSGKSTVVGLVQRWYELHGTTVLETLTDETEDSEKPESSAKEEEKPKKSKWFGKGDTPKANVEKDATKKLNDPKKGEEEVDLGPNTCTGTIRIGDTDLRKVDLKWWRSQIGLVQQEPFLFNDTLYNNVAYGLCGTRYEDFSKEEKLKMVEEACREAYAEEFISRLPKGLETLVGESGIKLSGGQRQRIAIARSIIKNPLILILDEATSAIDVRTEQIVQKALDQVSKNRTTLVIAHRLSTIKRADKIVVLRQGKPIEQGTHEELLKIEAGVYHGLVHAQELAMEVEEQTIEEPSLKKIQSTDIEETEAHERGSHTSELDPEYKNKGLIGSFGRLIFEQRHHWILYTIVIIGVLGGGVVYPLQAYLFASIINVFTLTGPELVRQGNFWAGMFGVLAAGVGLAYFILGSASHLISIQVTRDYRHEYLGNMIRKRIAFFDDQGHSPGSLTARLSSDCEQLQQLMSTEMSLALIAAVNLVGCIIISFIYGWKFSLVGLFAALPLILSAGYMRIRLEMQFEADTAKVFENSSQFATEAVGAFRTVLSLIMEDMIGDRYETLLKGHVDKAFAGAKYGTLIFAASDSIELACMALAFWYGGTLLASYEYGIVEFFVVYMAIVQGSIAAGMWFSFAPNIAQATGASNRILSMRPPQNAPPPNYTVLRNSSAGVGITFQNVYFTYKSREVPVLSNLNIQVQPGQFAALVGASGCGKSTTISLLERFYDADGGQILYDGQDITSLDPSEYRKQLALVSQEPTLYEGTISENVGLSVENANDDDVEQACRDAQIHEFIASLPEGYATRLGPKGMSLSGGQKQRLSLARALLRKPKVLLLDEATSSLDSESEKLVQEAIERAAGEGARTVIAVAHRLATIQKADVIFVLGSGKVLERGDHQTLLRKRGVYWQMCQAQALDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y289</accession>
    <name>A0A9W8Y289_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pig-Q</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gpi1_1</name>
        <name evidence="2" type="ORF">N0V83_008233</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y289"/>
    <dbReference type="OrthoDB" id="70250at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007720">
        <property type="entry name" value="PigQ/GPI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21329:SF3">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21329">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT Q-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05024">
        <property type="entry name" value="Gpi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="498" mass="56183" checksum="6220220DE4B32A1F" modified="2023-11-08" version="1">MHAFSIVTIGIFVAGYSTARWDLVTRLYELAIFAWDHGVVTRAAKGFAILSVFFFLLILPITRIAVQETDQHPRLVGGGISAREQLKHPLPGVLVGFKNSVSDVLVVAVLQEVELRHVENALVVGTLLRHSTYGVQELLQRCGHSSLRILGHVNPKTPSDHLDAGLITVYTEPSARLPHLYVAADTLTTAQIIIYDRPHPTRMQYLSLKPISLALGDGTADMERAVTSEELEETEGTERKRKEKLVEKLMLHKVISRPATQEELALPMLVEQVNCSHEINTLLQKNIGIIGRRMKRALSVSERVVESANGLWDQIYLVVNHVFGVWIWPTVAQVFIFGLITHRIMAEVVLRFLHWRPGPPGSPALKDISATAQQIDIRMQQSCYWPDQYLTLRKRKANWESITNSHPDYIRFYNSLWLVANDVIMGIALGTYIIENALFVAAQVDTIFNAWSVDGLRRMISWLMGWPGGLKLNTELADFLGDLFLWVIDYWAGEWTAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2T6</accession>
    <name>A0A9W8Y2T6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2T6"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4364538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="283" mass="32019" checksum="70A33426823CFD26" modified="2023-11-08" version="1">MANSQDDANHRCRVLIDNIFESLSTIVKSIPDSKDEEASIVKPLCALVEKAYHKVFQAYAATYLMIKDKDEVKKLEVKKLEESLQAAFDSLETYAESMKQYMLTPFRVWTSRLHNNRKSFELHFMACASRILPSKEYETLALFNASLHEEQPVRDSVLQPQLHADWYAQTFAVKTKKPELRSSVTSSSSNGSTSSAKSIDDMGYIDFKRTLSPEIMPIAPILQEGLGLTPAEKHINTAVKAFLLSQHRSLVKAIALEEKGSQVEKIVELDEPITPVRTEKVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y320</accession>
    <name>A0A9W8Y320_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">RRN7-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_009255</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRN7/TAF1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y320"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070860">
        <property type="term" value="C:RNA polymerase I core factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001164">
        <property type="term" value="F:RNA polymerase I core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048538">
        <property type="entry name" value="Rrn7_cyclin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048540">
        <property type="entry name" value="Rrn7_cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033599">
        <property type="entry name" value="TAF1B/Rrn7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021752">
        <property type="entry name" value="TF_Rrn7_Zf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31576">
        <property type="entry name" value="TATA BOX-BINDING PROTEIN-ASSOCIATED FACTOR RNA POLYMERASE I SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31576:SF2">
        <property type="entry name" value="TATA BOX-BINDING PROTEIN-ASSOCIATED FACTOR RNA POLYMERASE I SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20645">
        <property type="entry name" value="Rrn7_cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20644">
        <property type="entry name" value="Rrn7_cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11781">
        <property type="entry name" value="Zn_ribbon_RRN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRN7-type" evidence="11">
        <location>
            <begin position="6"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Rrn7/TAF1B N-terminal cyclin" evidence="12">
        <location>
            <begin position="88"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Rrn7/TAF1B C-terminal cyclin" evidence="13">
        <location>
            <begin position="240"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="130"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="156"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF11781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4364658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="542" mass="62643" checksum="A66209AA39D12632" modified="2023-11-08" version="1">MEKRSTKGPICGVENCRSRRYDEGEDGFLYCQNGHRQHGLVRGADDEDNFMNASRSITRKKKDADDSEKRVAKNFSGRQAFDLYLKCLQLILRHQIWFLVQEKGLPAELEIVVFDLWALRIAQFGDKIASNSQESDSQSQSQVFSTLDTEDSETTDNERDLHKRPQGRDRKLAGAPNLRDCLALCYLGILTLRLPITPGDIHAWVTDGKMPYMRAIKYLPLGMRDRLPPTYHAVLDSQKLLKYNRFYDVIINLQLSFNKDHGISWPALNVPVLLFRYLKELALPLELYDATIRLGELLDYDFATHHDGSKRLGLRHLPEAQLIGCLIVCVKLLYPFDNERRYPISAWEPTATVVNWKEWCKQMELAKTRQRGSADRFTVEELLKLTEQDVFSMSSDQLDQYLGYYAETFLDDAEVQRTKNNDDFRNALYEMFPVNDKGEPSPDSLANGLPLQDRLDTVKAVQNSMRSMTIISDDTAEPVILRPGQMYPWWKTAEDLPEEAKLFFEQAANLVGLSMKMLVTTVFFTEARIEQWRRQQTRQQRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y407</accession>
    <name>A0A9W8Y407_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein transport protein SEC23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">SEC23</name>
        <name evidence="19" type="ORF">N0V83_008956</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Cytoplasmic vesicle</location>
            <location evidence="1 14">COPII-coated vesicle membrane</location>
            <topology evidence="1 14">Peripheral membrane protein</topology>
            <orientation evidence="1 14">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 14">Endoplasmic reticulum membrane</location>
            <topology evidence="2 14">Peripheral membrane protein</topology>
            <orientation evidence="2 14">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Golgi apparatus membrane</location>
            <topology evidence="14">Peripheral membrane protein</topology>
            <orientation evidence="14">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the SEC23/SEC24 family. SEC23 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y407"/>
    <dbReference type="OrthoDB" id="10256289at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01478">
        <property type="entry name" value="Sec23-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11287">
        <property type="entry name" value="Sec23_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.730:FF:000001">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000006">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000008">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037364">
        <property type="entry name" value="Sec23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037550">
        <property type="entry name" value="Sec23_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141:SF0">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 14" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="11 14" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0813">Transport</keyword>
    <keyword evidence="8 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="16">
        <location>
            <begin position="69"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="18">
        <location>
            <begin position="348"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="17">
        <location>
            <begin position="466"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="15">
        <location>
            <begin position="582"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ4365335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="715" mass="79595" checksum="08821264C1B3E66A" modified="2023-11-08" version="1">MADFEALKDQWSEIEDRDGIRLSWNTFPSSRMRNSLPAHYKDISAEQIPPELHQGSTTIEYRLPQPVQTPPIFIFVVDTCQEEDSLKALKDSIIMSLSLIPQYAYVGLITYGTMTQVHELGYTECAKSYVFRGNKDYSSKQVQEMLGLSQMAPRANVPQQPGRPPVVPPGGAGTRFILPVSQCEFQLTNALEQLQRDPWTVANDKRPLRCTGVALSVAAGLLETNFLNTGARIMLFSGGPATEGPGMVVGPELREPIRSHHDIDRDNIKYYKKALKFYEGLAKRVSHNGHIVDIFAGCLDQVGLLEMRGLANNTGGHMILTDSFTSSMYKQSFARVFNKDAEDNLLMGFNASLEVITTKELKVTGLIGHAVSLNKKSVAVGETECGIGNTCAWKMCGIDPESSYGIYFEIASQGGPNQMQAGPQQGMMQFITQYQHAAGQYHLRVTTVARNLSGPSGDPAIAQSFDQEAAAVLMSRIAVFKAEVDDGPDVLRWVDRMLIRLCSRFAEYRKDDPSSFRLEKNFTLYPQFMFHLRRSQFLQVFNNSPDETAFYRHILNHEDVSNSLIMIQPTLDSYGFDHEGGQPVLLDSTSIQSETVLLLDTFFHILIFHGETMAEWRKAGYQEQEGYENFAALLEAPKEDAKDLIQDRFPLPRFIVCDAGGSQARFLLSKLNPSTTHTSGSYGGVAQTAQTIFTDDVSLQTFMDHLMKLAVTGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4K9</accession>
    <name>A0A9W8Y4K9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA-binding ribosome biosynthesis protein mak21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MAK21</name>
        <name evidence="4" type="ORF">N0V83_007252</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4K9"/>
    <dbReference type="OrthoDB" id="28947at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040155">
        <property type="entry name" value="CEBPZ/Mak21-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12048">
        <property type="entry name" value="CCAAT-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12048:SF0">
        <property type="entry name" value="CCAAT_ENHANCER-BINDING PROTEIN ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCAAT-binding factor" evidence="3">
        <location>
            <begin position="449"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="550"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="724"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="552"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="573"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="740"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="763"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="799"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="851"/>
            <end position="861"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4367667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="891" mass="100844" checksum="047C5D69A4D4C7A9" modified="2023-11-08" version="1">MGKKRTHAETKDGFTKPPPDKNRLSVKNDKKDKRKNGEVQKPRSQLLFKAQPEWHSVELPELPTVDNPTIPPRHVIDAIREYADQLLEAEATEYASSHMAKDSSHKFMSTVMSSGTMEDKVSALTLLVQESPLHTQKAFGQLLGLSQKKSRSAAMMALAALKDLLGQGVLLPPDRKLKAFARQPGLISSLQGKGAHWKAGEKLPGHLQKIHLIVWAYEDWLKKQYFEMLKILETWSNDEVEYSRNRAVTFVWELLKEKPEQEENLLRLLINKLGDKEKKVASRASYLLLQLQITHPLMKNVIISSIESDLLFRPNQSGVAKYYAVITLNQTVLSVKEPEVANKLLEIYFSLFLGLLKKQKEHEREEKRINKHGHIQGGGGQPGKMAKKKAEKAATMAFKVEDESREKLISAILTGVNRAFPFAKTDDAKFEEQLNMIFEVTHSSNFNTSIQAMTLIQQISATKHYSADRFYRTLYESLLDPRLISTSKHIMFLNLLYRSLKADTSIKRVKAFVKRLLQIIHMHEPPFICGVLYLVNELITTFPTIKSMLSTPEDHADDSGDEHYEDVAEDGEDSKGKEEKKSTHPTYDARKRDPEHAQADLSCLWELLPLQAHYHPSVHVLASKIVNQEPIKEKPDPTIYTLMNFLDKFSFRNAKTKVQGVHGSSIMQPMSGTSKAASYLITARDGDRTHDPLNSEQFWRQKVDDVRVDEVFFHTYFEKAGKAKQADKKSKKRDKRKGDDSEEESGDEDEIWQALVKSRPDVEGDAGDDEDLSDFDMDDMMSDEEAGGMDGGVELNLGSDDDAVEVDGQPADDDDDEDDFENFDLDDEDAFVDSDADVPVDIDMNDDTDVDEAKKGTDKDGRKAKKRKLKNLPTFASVEDYAKLIGDDDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4Y0</accession>
    <name>A0A9W8Y4Y0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_006474</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4Y0"/>
    <dbReference type="OrthoDB" id="3945418at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF157">
        <property type="entry name" value="N-ACETYLTRYPTOPHAN 6-HYDROXYLASE IVOC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="20287" checksum="AB726E57A7AD8644" modified="2023-11-08" version="1">MKQDNDRRRIKTDDRGSVFLHLANSDMPASERAPERLLKEAQVLLAGGTATTSHTLGEDLREELRKPMADWPSHIPTLVELEKLPLLTAIIKESLRLAYGVMHRLPRVSPDTPIQYGKYTIPSRVPVGMSTYFMHTDPDVFSQPFEFKPERWLGSITPKMLRNYVPFARGSRNCLGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4Y5</accession>
    <name>A0A9W8Y4Y5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_006386</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4Y5"/>
    <dbReference type="OrthoDB" id="9992527at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08249">
        <property type="entry name" value="enoyl_reductase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047122">
        <property type="entry name" value="Trans-enoyl_RdTase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348:SF3">
        <property type="entry name" value="ENOYL REDUCTASE (ER) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348">
        <property type="entry name" value="HYPOTHETICAL OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="5">
        <location>
            <begin position="12"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="376"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4368031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="410" mass="44781" checksum="C2873D5D72D6F7B3" modified="2023-11-08" version="1">MSTTHAAVATVALKAPLAIIQVPTIKPTGEQVRVRVEWTASTPLDVHQNDGGLLVTHPQVLGDGTAGTVVEVGPSVKNLKVGDKVFGFTWRSQAEKAHQEFCTAEEWLFAKLPEPYTLPEAVTLPNNFVTVFHTLATDLNIPTPWPKSSNYIPEDANQPILIWGGSSSVGQFAIQILRYYGYTNILATASSKNHDSLRALGATHLVDYNAPDVVDSLLPLLHQVTSTSSPAAEGIAPLVFDCIGSKHGSILPISKVVGKGAKVAVLLPVIVRDSSEEVDPEYEMDVAASAEWAEGLEARGVRTHSYLDVSNSSPHPTSPSNLPSWVTPIEKRRIEASLAAGADMLMQNEFFKYHLQPDIMHSLLRDGVVKPQRQRIVEGESLLERAQRALDMLRRKEASMERLVWRVAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y509</accession>
    <name>A0A9W8Y509_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Asparaginyl-tRNA synthetase</fullName>
            <ecNumber evidence="7">6.1.1.22</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SLM5</name>
        <name evidence="7" type="ORF">N0V83_008397</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y509"/>
    <dbReference type="OrthoDB" id="43906at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF34">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="6">
        <location>
            <begin position="16"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4365776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="289" mass="32152" checksum="E13D299B3E508470" modified="2023-11-08" version="1">MLPQLHLEALALSVNKVWALSPTFRAEKSDTARHLSEFYMLEAELAFVDDVKDVMDVVEDMLRAVATELQTSSVGQELLEARAREQEHEGASISHVALAQRWQGLVDGPWPRITYDLAIQHLKDAVAQGKVKFEFAPGHDEGLQTEHERFLAESVGRGGPIFVTDYPRNIKPFYMAPSTDASTEGQAAPTVACFDLLVPEICELVGGSMREDRSPQLLRSMSEHGLQATNDTPEAPGGSLQWYVDLRRYGSVPHGGFGLGFDRLLCYLSGVSNIRDVVSFPRWYKRCDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y571</accession>
    <name>A0A9W8Y571_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_006965</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y571"/>
    <dbReference type="OrthoDB" id="303107at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031213">
        <property type="term" value="C:RSF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028938">
        <property type="entry name" value="Rsf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14296:SF3">
        <property type="entry name" value="DIKAR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14296">
        <property type="entry name" value="REMODELING AND SPACING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="430"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="181"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="312"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="489"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="351"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="502"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="619"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="637"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="693"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="706"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="716"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4367383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="820" mass="91624" checksum="EA25FEDF2FE175AC" modified="2023-11-08" version="1">MPARKRAASEMEAEEPVEEPSTLQKLRNMWQFANLAQYFSLFGDAVKIDKDMDIEALETECLKPQPSEKLAQIGLALLKHVSSHKGLTPDIFDEYTRRQFVVKAPARNPFGTDEEPNKFDAFDIFTKIRVLQQLSTWTLNNPNTIRERIAATDSEQTLWRMEPTGWDSQERMLFVLDDNRMYRLTDPPPPPAPQKAKSKAKSKKSKGRRASKRQKQSTPEPEEPSEDEEAPGDQQVEEIDDGLGGKKWECVCVTLEDYHEYMTSIRKSRDPNEKILYKRLQEDVIPVMENLAEEQARKQARKMRELENMQKLAMAKRSSRISSRLEKQKEVEEAESAERKRQAELAMAQAEQERQTKLEEAHDSRRMTREQRLRERETAKILKEEELRRLQENEQKLASNNARLSERHLKAMMKKHESDLKRLNEEEDWFFDCEKCGTYGSNLNDNTPQISCEKCNVWQHMKCHGITEEQAEDSKFVFVCTSCKRKEEEANQPKLPPLKLRLTSTSPSSRNETQANGAGSLTRQLEAVKLPAPRPSTSQAHEQPAQPVQPWVDGPSLSPRGQALGPPGIHRSEAAYGSPTKGANGSSSPILARPYSSGQPAGGFKPFSLPTSSPPPYNPQQIPSSPNKSFHMPHPQHNGNSFGSSNPFVSSQPIPYTQSFNRPTSAAGPGGSPVKHSPAASPRPANGLPTAYNFNSPHSSFPPSSAQRTSFSPTKHSSPAPLPAHMSSPAPAPMRIAPSPSQMPAQMLPDPIPAPSKHDGMRPVSSHAMSETPIFPPAKALSPQRNPQILEPPTKKPSPTPERFQVAPVGQNGFANGSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5D6</accession>
    <name>A0A9W8Y5D6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_008034</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5D6"/>
    <dbReference type="OrthoDB" id="187139at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31736">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31736:SF9">
        <property type="entry name" value="ENDO-XYLOGALACTURONAN HYDROLASE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4366398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="305" mass="33348" checksum="8CE88940E758E30C" modified="2023-11-08" version="1">MYCRLTQRPLVDDVPSILSALQRCGDTGTIAFPANQTFNIRSPLDLSSCRRCGNQINGNLNLSPDWDYWVEAPAVFLISNTSNMVMSSSEGNTGMIDAMDFGWTRVPDVPVPDRMPNLFSIIDGSYQVYIRNLKIRYAPGTVFHVGSESSAIRFEDVEIQSEATTGYLIENAQHVYIWNSIVRATGSCVKIAPNATNIQVEDVRCYTVALNGPPPSEIELTTSVTGAASWIRNIFVNSFVGTGWMNVVAFAAAQADLAPQSLEIKNATFTDISFGDEARQAVYVEQSSAPLTATVITFRAFTGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5Q0</accession>
    <name>A0A9W8Y5Q0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_007123</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5Q0"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4367540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="394" mass="43443" checksum="8D23F4D3BA36856D" modified="2023-11-08" version="1">MNEYDGRARMCPNGDWGWISSHTAAGEVGDDITIEEENTEGIDDVDDGNRTSVFQARQTDAEADFNSAFGAFDFADDRSTSITAPQSTQCPPFRQRLQYDDYDFQCQQRFTVYATSHKAPSWGICQGTPSRPSENCVNEFAVSQQRERVAANQRPVQINDHFTIFQGSGPHTSRTPHRSHGGEEVLSAYSAENAQLDFAPSMELGSQQYGDRVAGYENGPNTRSSSSFDLPSHVYTNLDAEDQVVDFTESDISMIQQQGPWSVVNSRQDERQVCLYNETNQLVNLISVSGSSNLSLYGQDFSSLVPSDDPETSATTLSSEIDEEMEASWATIRPGAEPVLNQSSSTFQFGNQHAEAPTNTLAPWVDRSKTGICVQVREGTPDVSNVPEVVSYSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5T1</accession>
    <name>A0A9W8Y5T1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005950</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5T1"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054471">
        <property type="entry name" value="GPIID_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF16">
        <property type="entry name" value="GPI INOSITOL-DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22939">
        <property type="entry name" value="WHD_GPIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GPI inositol-deacylase winged helix" evidence="2">
        <location>
            <begin position="100"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="1">
        <location>
            <begin position="516"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="927" mass="103519" checksum="24191E52BB5FD4D6" modified="2023-11-08" version="1">MIRQLDGCATTVVHIPERDTRKDIEQYVRSRIVDVAIDEEDEREELARRILAKSGTSFLWVRLVMDELEGLYGYESIMQVLQGIPEGMFSYYGRAVAEIEQNRKERHVAMAILRWIMLAARPLTTSELSQALQLDIKVHLSSAKSAIEGLCGQFVSVDLHTDLVHPIHATAREFLLSEEAGRLRIAKAKGHESIALICLRLLASPAMQPPRHRRLLDQKRVKPPSSVLLDYAITQFSEHIFSASSESDQLLVALDKFLITSVLTWIEHVGLKKETHRLIRTAKNFKAYLDRRAKYHSPLNRHVNNIELWATDLTRIASNFGRALSSNASSIYFLIPPLCPSQSAVYRQFGQSPDGLKLLGYHLSDWDDCISHVQFEEKTAAAVSCGSGVIAIGTESGKVDIYNHRNFQKDTTINHGFPIDLIWFDPLGSFIATCSRKVVALWDLDGNLRWKMRIKSRCILLTSSTTSLIGVTQQGRSFQWEIDTGQLLEEQSYTFQLPDMESAYQGSTAKAPSSAALSPEMDLLALVYRNNPVCIFEFGSGSMIGWAVDDNSRAAEQVIFNPNPEVNLLLVAYNESHLALYDPWSGALVDSLEADKSAIVTSITCSPDGRTFAAMDILGHLRIWDFESLTLLYHVLTPNHSFSLLSFTSDGLGLVSVFEHEMRVWAPSALVRKTIEEEASTSDQATVLPVTEGQFERFHTSKLRTLVAHAHLPAYFTSNHSGEIVVYRSQDNYQPSVLYSHDGVLVKCVAISSANLIASADVHAKIQVWRLSSNSLQVEQLIFQAQFAAPIFQLLFDTSGQHLLVSTTEQDHVYNVETGALTGTLRFGLEKRSAWKWFVASKSGYSKSFLLVTDCTLASYSVEDFPARAQIAPMPLEYPAHDASIITEIDSISFSVETSTIAIEVRQQQENARISQANSKAEPKSVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5X4</accession>
    <name>A0A9W8Y5X4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006767</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5X4"/>
    <dbReference type="OrthoDB" id="2157530at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF73">
        <property type="entry name" value="HET DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G01020)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26639">
        <property type="entry name" value="Het-6_barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="2">
        <location>
            <begin position="44"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="490"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="606" mass="68196" checksum="DA3A743D259D5A3D" modified="2023-11-08" version="1">MTQIYKPLDDSKREIRLLHLQPGDDEAELTCFFSLVSLDDKPDYEAVSYVWGDSLDRRRINVEDAKEPVPITRNLHSALHGLRLQDRERILWADALCINQQDLEERACQVAIMAAVFGQASRVLAYLGDDWDGCDLAMKAIRQLGEDPTMHLFSYMDHGLSIDGIGFESLALLDVVSRFLDTPWITRVWTVQEYSVAKEVTFLYGNSAISAAAVLGMCLGTAQHTWSCCSFPSQPFTRIAAHLDAMQRWSSLKMKCSTLQTKTYVDPVSCLMDLELFRDRLATDPRDKVYSLLGITRPSWAALIQPDYSIEPGETFMRAAIADLQITGHLHFLNFCYMSSRSSRPSWVPDWTISSSNWRLYQQRLVLATATKACDGRTAQVEFTPYNALIASGILISTISTCSSPEIEIGRTLPKQDFHDILREMHEIVGVKEHSTDIYGATSQSILEACRLALVGGWIDNAISGYEPIYAVEDVEQFEEWEAFNGCDTTSFPTHGRPLRKKQQDGPKANDDEERRKGLWRLKNTILINTFHRRFVMLAKSYFGFAAENCQEGDVVVVLNGGPTPYILRPVPGSTEYTYIGDACIHGMMNGEAFGANRPPDKFTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6E6</accession>
    <name>A0A9W8Y6E6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_006641</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GPAT/DAPAT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6E6"/>
    <dbReference type="OrthoDB" id="10255570at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07993">
        <property type="entry name" value="LPLAT_DHAPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022284">
        <property type="entry name" value="GPAT/DHAPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045520">
        <property type="entry name" value="GPAT/DHAPAT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041728">
        <property type="entry name" value="GPAT/DHAPAT_LPLAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563:SF17">
        <property type="entry name" value="DIHYDROXYACETONE PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19277">
        <property type="entry name" value="GPAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="7">
        <location>
            <begin position="217"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="810" mass="90849" checksum="D8F7E8273AB9E4ED" modified="2023-11-08" version="1">MGSSNKKRKPRASSSSTPEQAPDLSITADDQVYLHAAGYNESPDRGLINSMARFRSSPLDFIREVSLHFSGTGWRSFDDHIGQPEFYSGFSEDMKTRVLSSPMLVAKVRELAAKRVEMEAKEGLLGEEAHNGSGGVGEKDRKVKRRVQIEESLMEVSETWTDNMICKMESKNFIRGAYYLATQLLTRAYHQGVHVSSEEVLRLRAVADKAAKDKHSIIFLPCHRSHVDYVSLQIICYRLGLALPTVVAGDNLNFPVVGSFLQHAGAMWIRRSFGDDQLYITLVQAYIDTLLQSGYNLECFVEGGRSRTGKLLPPKFGILSYMLDSVASGRVDDAIICPVSTQYDKVIEVDSYISELLGQPKPKENLMDFLSASSVLSLKLGRVDVRFHEPWSLRTFINQQRERFSQLPQQLDTKEDRLRLLRTLGYKVLSEINDVSVVMPTALVGTVLLTLRGRGVGKSELIRRVEWLSDRVRAQGGRVAHFGNLPTSVVVDRALEVLGPGLVGLVPGLPEDTYYAVDRFQLSFYRNMTIHLFILQSLVSAALYTHVKRGGGPEYQRISYTDLHAQVHFLSQLFRGEFIFPTEGLDHNLAKTLEGLEADNVIKIQRGKDLNGVEKVEYVELSDAERETGRENFDFYCFLIWPFIEAAWLGAISLTMLTPPLTHSSSSPFPSSTSLELKKVQDRAQLFGKTLYHQGDLSYFEAVNKETLKNAYTRFEEEGMIVVTKARDKNTPATIKLADEWMPQRGMKGIIAEGKLWRFAERISASRREGKNRRDGATVQTRVLELADKVGGGCGRMIVGCFWRRGGGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7J2</accession>
    <name>A0A9W8Y7J2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006287</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7J2"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="202" mass="21384" checksum="6495256E2741940B" modified="2023-11-08" version="1">MAANDYYTTHPQNPTTFTHQSTFHGNASNGPANLTATNTSYEPYRRRALSQLPHYQQQQQQYPLAVPTGSSSPPYLSTSLPSDTSSSTSSTDRSLFSSSFGSSGGGEKKSKSERAIKFVEKQVKKQIEKKLNGGKHGGQEQQEQGCEDYGGIGQQDQSGGGAYGFTGNHGGGDGAGKFDWVQDAGGWFGDLDSGGLESVLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7R7</accession>
    <name>A0A9W8Y7R7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nuclear pore complex protein Nup98-Nup96</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">NUP98</name>
        <name evidence="2" type="ORF">N0V83_005878</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7R7"/>
    <dbReference type="OrthoDB" id="3797628at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034398">
        <property type="term" value="P:telomere tethering at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037665">
        <property type="entry name" value="Nucleoporin_S59-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198:SF6">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP98-NUP96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198">
        <property type="entry name" value="NUCLEOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="465"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="579"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="294"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="465"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="488"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="596"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="665"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="683"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="763"/>
            <end position="789"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="789" mass="82854" checksum="A8244E2C44C1E5EF" modified="2023-11-08" version="1">MRADHNILTWASNQWPSYRATSNNYEAYHRQVQPAMGSALGKDDALPSVDTLQITPSSTREGTPTDETEDGVAAGKPDLPGLTASCSASADKADIPTSAVSPQASQDPTSTEQKDKILSIASETIATSRADAGKAPLNFTCGPADHLEAYILRPHIDTVDDRIIWSAKVDQLYLDKDALQAHMSKLGPDYSIIDAMSELSPDQIQLIQARTKSRNGFLVAIQYSASKDMVTHMGTFKIQPIMFIVKITVLPKKKELPWPPLGTPQPVAPGAPRIPPVPLPSWALPPPAPVTSFDAADASSDSSDTSSDSTDTPRAQLGKHYFFRVEKNPLYEGGYMTITAQDKYTGHGNERSLEELRLEDYEAGRTNATKASLLGYGSSFGFQSATPVPRRAGGGLFGAGASGGFASPVQQPQQTRDVFGQTSGDGLFSVKPAPGGGLFGGNIGAHQQRQHTGGPFGRAPGSGLFGGAPSGGGLFRGQPTPTGGMFGSTAPTSQQPQQSRGLFGESLSAANPSSGFSPRNNAGGSLFEKLTSPAPGQAMFGQATSIPFTRFPATSGQRAYTKGWVPTGIPPQATLSSFFTSTQTRPERDLGPSPFAALGTKPPTTATAAAQPTPAAPSTSSNPFAAPPAPVAAPNDDSRSINPFPRAPTPHPSCWHCEEKLQNDAPPGAAPTSATAATSSETAVKKDEAEKEKAVTKEGDGKDTEAKKKPRHVRFVVNEQGVVSSPPGIRPALPGYSPPAQTQPLPTPQVNPFRGFGNVVRPGDLRLYRNGGDKGGDAEKEGKGKDGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7S1</accession>
    <name>A0A9W8Y7S1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_006540</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2095648at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="389"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="980"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="547"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="623"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="739"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="850"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1086"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1488"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1839"/>
            <end position="1877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1900"/>
            <end position="1995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="747"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="851"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1494"/>
            <end position="1512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1529"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1844"/>
            <end position="1853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1861"/>
            <end position="1877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1927"/>
            <end position="1936"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4368184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1995" mass="219449" checksum="95B2E1F16098FD90" modified="2023-11-08" version="1">MDAPSALTNVTSYLPSPADLLMVFPRLFSKASYLGDMIRSGGSVIAEPTAVNVTNSTITSTAGQFVQESVAAAASASGSQDEISMFQAVKNIASFFSYMTSKWAIATFAIAILLNRTHFYASSRVALSFDRIHLRLALYITPLLLFLFQIHSVLQAIRCQTSPGWSEMQYGAPDRQLDTDFGGEGGYLWRAASMLLFWETTEQSCRAVNMLPADPESTRAAGSLALLWPLFLSIGYGQFVETLSCALQGRHPIQEAGMTIFEHSLAFAEAEAVVTKPLAVDSIRFFKPKSVFTPDGTSFLIPRSRLSKIANVPPEVLLISLISSISHFTSNTLAIAGVRSRYRLITTATWGLAYMSTFAWSFSRLTAMITDPDQHVGILRFPTVCIVGFIPHLLILLGIAACGSIYLLALLITVLSPPPGQPESISLRERFAVAYGNLHANIHLSAITPLTINWHEDFYTAILKVGFTVLTAASEAVFLNEGTKVNVHSMTWLERQRLQDILARRRQFRQSLTNIPHELKNDTLATGVEIVDNVDGVAAEEAITSGYANERKARSPNSASDATRPMGPNDPAGLQQRRSRFYLTYHFFKSISKLLGLIHARLTIALLRKLRITYRPRWLRRLAGPPPTDKNRASGSGSGRSQYRAAAADEPWLVLDGRIVVRDESNFDIESFARERLRQTGFYANLDAQGIEDRINDYLYSWWRNDGRWGDIDTSSDYNPSYDDDDTTSVVSYATTDNEAWSDIDDDGQRTPTRDTYQRSREETPLVDDAMDFSRLSQLLDPKTKDDREEAKLLARHLQSSKIMTRSQYRKMLERDEAKVLTSSRYKMGNTIKMSPEEEEELLEDLILSKRSATAPQGSSTTGSWDTGAEGMGSDGPQCVHGQQRDDDLQASLQPHSGGPQGESEPPTGGNARDATRSDPVSDDDDTSEAVRLKGTTNAISPPRNRIAEYENALASSPPKPSEGRLFEVTKSNRRPDDKSSPIAKLPNEVLIHAIAHLSPNDLAAVSLVSRRFNDIITTPHAWQVAFARYFPGPQSLEDAAFRSALENNGTIVRSEKRRFTRLTALASWRSEYILRTRLLRSLARGKPVQNSSTPSRAGHAQPGLSAPTIMYDVRTYTIINHLHGSFGSGLNKRIPRFIHGADDVGMVTSSDPLTGKVDPWGQSDPNFYGQFSERFPGVAEWGLGSGEVVGRPNVMDVSQPYGMIYGQGCPEGSCYYRSLEEMRGRFIAEPTEFTMPETGIPKLGIDGGAICSVWIAKSNTIPSLSEGLIGMMMGSAAGIITACSIGTDGLRTSRVQRGELTARWVLSPGVPIIAIAVDEQYSLKRHAQNRIWAVALNALGELFYLTKFPTRSQATKGNIEEYTEYLAWLTGRSVYWNLVEPSRRTARPDPYGDAEFDGSYSPRTSWDGMCLSKEQVTAETREIQEFLRKPPKQFRKSCLGWDMRRRLEVDFAGDDGNYAGEAMVVFQCGLDEGDVADVTRYTRWRTNDKDKSTSTSTRISTPQPSQTTSLFGGSGTGTPDVVRIPQRARSSSYISTGSSPERVSLAEEWRCSKLTFGGFKNTQLATTTLDISTFATLTLSEDPALGFSTASTASSPYASPMSVASQPASPSDIPGQRARFIAAGTKKGVVILWDVRSPVSRSSEYTNNIEPVRIIYTESPEISCLAMTSLHLVVGGNDGLVQAWDPLASSMSPIRTLNSKHSSRARRRLVQAQASVQGVGINMFAAGAVCLDPDPTVLRGAVSLGNQLLYWSYSSSAADQYKSSKRRLRRSERGSNNAVERFSGTGRANLKDYIANEKYEMEREKVERRKQAERLAGRFGTELLSEEEALAYAAMLSQESMEAEERRRRESESSALSSETVTPEPSVFGQSSPTLNNHQELDADIAEAIRLSLNEDATSGLYEPSSTPPSTFDIPIKYAKGKKSSPSRANDSPTNKGKVAAGSSNQNELSDLEFAMQLSLAEEASRKETEDAFPPLSPASGGGGGKWKGKGKMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y885</accession>
    <name>A0A9W8Y885_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PRE10</name>
        <name evidence="6" type="ORF">N0V83_004965</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y885"/>
    <dbReference type="OrthoDB" id="40134at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="1 2 3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="5">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="228"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="256"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4370447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="268" mass="28836" checksum="EEB702BA8DEB71E6" modified="2023-11-08" version="1">MTSIGTGYDLSNSVFSPDGRNFQVEYAVKAVENGGTAVGIKCKDGVVLALEKLVTSKLLKPGANKRIATVDRNIGIVFYPTVVTSYHGPAMRQHSGGSFTRPQSPSSSLADRMGSYAQAYTLYSSVRPFGITAIIAGWDSEAELPVDAQVGSGPKVGSGGKTEGAKHGGPSLYMIEPSGLYWGYYGAATGKGRQIAKSELEKLNLAEGELSLEEGVKEAARIIYVAHDDNKDKEHQEVPKEIKEEAERLAKKSLEGDDDEEEEEKMQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8R1</accession>
    <name>A0A9W8Y8R1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ecp2 effector protein-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005363</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8R1"/>
    <dbReference type="OrthoDB" id="73875at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029226">
        <property type="entry name" value="Ecp2-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14856">
        <property type="entry name" value="Hce2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040923723" description="Ecp2 effector protein-like domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Ecp2 effector protein-like" evidence="2">
        <location>
            <begin position="33"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="145" mass="15113" checksum="9729C51251FA72ED" modified="2023-11-08" version="1">MQFQLLPVIAALLAATSASPVASKLDTRGSINDCGDSTFENQSSDGSPNISDCQQIATNIAGGGTWTVAVGQHQLVQYGTCAFGVTPEDSLNYAYIGNQDIIDVINASIQKFSYNGKVGAKGVMGCQSGQGEVSGDEVTWGIYHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y965</accession>
    <name>A0A9W8Y965_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pre-mRNA-splicing factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">spp2</name>
        <name evidence="7" type="ORF">N0V83_005180</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in spliceosome maturation and the first step of pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the SPP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y965"/>
    <dbReference type="OrthoDB" id="5577072at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045166">
        <property type="entry name" value="Spp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026822">
        <property type="entry name" value="Spp2/MOS2_G-patch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15818">
        <property type="entry name" value="G PATCH AND KOW-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15818:SF2">
        <property type="entry name" value="G-PATCH DOMAIN AND KOW MOTIFS-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12656">
        <property type="entry name" value="G-patch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="G-patch" evidence="6">
        <location>
            <begin position="207"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="24"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="298"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="333"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="412"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="438"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="463"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4370659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="477" mass="55504" checksum="C0BDA9C1F949ACC9" modified="2023-11-08" version="1">MAAPKEGGFKMSLGGLKAKPGLKAALAQKPAKRPRLALDDDEPDDSNKQQEILGWDAAEGGALEANGKKEKEGPRIIPVPSNHDWRSEVLRKRLANAPHQQAQNGVDVDKIVEPKVQYGLTISKKDEPQENGVAEPAVPVPEPMEDVKDNLTEEQRLEKKALDALLTGKSSDQDLVIPAMTEEEVFRHDLEDAPDAPTLETYQAQPIEGFGAALLRGMGWKDGEGFGKNGPLPVAKEVKRRPALLGIGAKPEAALGIELGEWGSKSKGKGKKVAQVYNPVILRNKRTGEMLTEEELKAKLESQDIVNDEPAKSRSKYDDEEEEDERRRAKRREKGDDKDRDRDRRREKDYDSDRRDKDRREKDRRRERDDYYDSDRRRDKRKDREDDYDSERRKDKPRERDRDVDYDSERRRDKRRDRHRSRSRSPNERKEKRRDRYRSRSRDSDDRRKRRKDTEYAYDDGDRDDRKKRYKDEKYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9L1</accession>
    <name>A0A9W8Y9L1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">2-oxoglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="5">1.2.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">2-oxoglutarate dehydrogenase complex component E1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">KGD1</name>
        <name evidence="18" type="ORF">N0V83_004402</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 2-oxoglutarate + H(+) = N(6)-[(R)-S(8)-succinyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:12188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:20092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="1.2.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9L1"/>
    <dbReference type="OrthoDB" id="413077at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02016">
        <property type="entry name" value="TPP_E1_OGDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1150:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11610:FF:000009">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12470:FF:000003">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11610">
        <property type="entry name" value="Multifunctional 2-oxoglutarate metabolism enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1150">
        <property type="entry name" value="TPP helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042179">
        <property type="entry name" value="KGD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="ODO-1/KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006914">
        <property type="entry name" value="PRK09404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008907">
        <property type="entry name" value="PRK12270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF4">
        <property type="entry name" value="2-OXOADIPATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="17">
        <location>
            <begin position="670"/>
            <end position="880"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4371186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1047" mass="118669" checksum="3AC2512CE527F9A7" modified="2023-11-08" version="1">MLRIPLRQCSRKLQSCPQRAGLSTLTASPSRTAISTCRRPLALSQRRKYAIAAEDTNKGVDPNDSFLQGNTANYVDEMYMQWKRDPTSVHYSWQVYFRNMESGDMPVSQAFQPPPTIVPTPSGGIGPDFKPGMGMAAAEGSDIMNHLKVQLLVRAYQARGHHKAKIDPLGIRTEAEQFGYSKPRELELSHYNFSEKDLDQDIELGPGILPRFKTETRKKMTLREIIEACERLYCGSYGVEYIHIPDREQCDWLRERIEVPQPFKYSVDEKRRILDRLIWGTNFEAFLATKYPNDKRFGLEGGESLIPGMKALIDRSVDYGVKDIVIGMPHRGRLNVLSNVVRKPNESIFSEFAGTAEPNDEGSGDVKYHLGMNFERPTPSGKRVQLSLVANPSHLEAEDPVVLGKTRAILHYNNDEKDARTAMGILLHGDAAFAGQGIVYETMGFHSLPSYHTGGTIHIIVNNQIGFTTDPRFSRSTPYCSDIAKAIDAPVFHVNGDDVEALNFVCQLAADFRAEFKKDVVIDMVCYRKQGHNETDQPFFTQPLMYKKITEQPQTLDIYTKKLLDEKTFTKEDVDEHKAWVWGMLDESFNRSKDYQPTAKEWLTSAWNGFKSPKELATEVLPHLPTAVEESQLKHIAEKIGNAPEDFNVHKNLKRILAGRTKTVVEGKNIDMATAEALALGSLCMEGHHVRVSGQDVERGTFSQRHAVLHDQENEKTYTPLQDLSEDQATFTISNSSLSEYGVLGFEYGYSLSSPNALVMWEAQFGDFANTAQVIIDQFVASGEVKWLQRSGLVMSLPHGYDGQGPEHSSGRMERYLQLVNEDPRIFPSPDKLERQHQDCNIQIAYTTKPSNYFHLLRRQMNRQFRKPLILFFSKSLLRHPLARSSIDEFTGDSHFQWIIEDPAHSSGDIESHEGINRVILCTGQVYAALVKEREARGLKDVAITRIEQLNPFPWQQLKENLDSYPNAQNIIWCQEEPLNAGAWSFTQPRIETLLNQTEHHNRRHVMYAGRNPSASVATGLKNSHKKEEKDLLEMAFSVKQDKLKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9U1</accession>
    <name>A0A9W8Y9U1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4371968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9U1"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="258" mass="30367" checksum="B74D8B4D488976F4" modified="2023-11-08" version="1">MAREEGDQTRKAERTRAEEEEQRQRAAAEAEKAAKKREEEEEEVEKRRVAREKAVRAREAQRRAAEQERIATEQKRNEAEERLRQLKEARAEQKSRQAAQRARQEQEQAAAERLRRDREREAEQRSSHRVLRQARWERQAAARERLLRLNILLGEQRQAAPRQKRRPEAERRRQHAEAKSESTVPVRPEFPPQTQTQRSGEECDHRQLLWLWKKGRTDCDVCGKMCAKSSFCCPKCGMAVCQTCKDRLGVHVVKSPMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAJ8</accession>
    <name>A0A9W8YAJ8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003762</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4371989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAJ8"/>
    <dbReference type="OrthoDB" id="3915128at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="486" mass="53392" checksum="B8E13B18FF8FA60D" modified="2023-11-08" version="1">MVRLFVPEGEEQHALTSRRPGALTAEPSVTAVPLSSALAATGLTGVLALVGRARPGTFLKCAALAGTLGLSYTTCKLYGQRELNRGVFKLDKIEAQPGKLWESTEHWTVEDATLTGGGLGVLAALNERAFPGAHGWQRFLGSALVGAALGSVLGQRIFVQIPPRLISKIDQTQAMTRHMQYERLQQNEAAKANLSRFGKLALRFYTWPPHQLLRELTGAEAGRENASSGAQGASPHSASSFLGLQSQNTISLEVEFEQGELNGPDMTAGVRTYQDSLRTRNARALEAYLAQLSTMRTTMVQEARYVWHVLAEKEKAYYAMVGESDEKDMCRRECDLLGNAASQCVMREAVISFHIADARKQLEQIPGRINKTTSASNDTTLLLDETPDTHYNPEFTTKHLRAHWTKQKAALAQVEQILSQYEVAVKQNGDRFLRAPGEIHGRVKAARVAVENLRGYLEATSRLLSEFEERGRKAEKMKGKGGSWGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAW3</accession>
    <name>A0A9W8YAW3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAW3"/>
    <dbReference type="OrthoDB" id="3695683at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="172" mass="17394" checksum="F1B320C2C73D212E" modified="2023-11-08" version="1">MFATAGTVNPAPPAALEPEAHPQPPAASEPPPEPAPEPAPEPASEPVPPAPEPTPGPEPAAAPDAPPAAPPEPAPEPAPAPATEEPAPAQPPEATPAPDAIPKDADTPLAPGVPSRTATTATTKQRKRDKIKAKAAAVRCPKEAAPIVLHLRQKQKAGEDIKPIVETMMKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBM4</accession>
    <name>A0A9W8YBM4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RTA1 like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_003559</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBM4"/>
    <dbReference type="OrthoDB" id="3358017at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007568">
        <property type="entry name" value="RTA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465:SF35">
        <property type="entry name" value="RTA1 DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04479">
        <property type="entry name" value="RTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="199"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4373265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="273" mass="30284" checksum="1D768DCE88884A16" modified="2023-11-08" version="1">MSDASAPSKYVLWPYTPSIAGGVIGAIVFFSLSGIHIFRLVKNRTWFCIPFVIGALFEAIGYSGRAAAHSDTESTTPYIIQSTLILLAPILFAASIYMILSRIIQRTDSAEYAIVRASWTTKIFVGGDLLCFCVQGGGAGMLIKAKDDAGFKMGENIILGGLILQILIFGFFVAVAAVWHRRLLARPTAAAAELPWARYIWFLYASSGCITVRNLCRVIEYALGKGGYLLSHEWPLYVYDFLPMMLTLVVCIAWYDPNIKPRRKSDIEFATRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBQ8</accession>
    <name>A0A9W8YBQ8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sm protein F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_003135</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the snRNP Sm proteins family. SmF/LSm6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBQ8"/>
    <dbReference type="OrthoDB" id="409625at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034715">
        <property type="term" value="C:pICln-Sm protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01722">
        <property type="entry name" value="Sm_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016487">
        <property type="entry name" value="Lsm6/sSmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034100">
        <property type="entry name" value="Sm_F"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006609">
        <property type="entry name" value="snRNP_SmF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 10" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 10" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="8"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4372844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="93" mass="10337" checksum="F684D1621CC2B758" modified="2023-11-08" version="1">MATVAPFNPRPLLQSLVDNDIVVRLKWAETEYKGRLVSVDLYMNIQLSNAEEFIAGVSSGTLGQVLIRCNNVLWIGKADEVEPKGWKDTNMTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC05</accession>
    <name>A0A9W8YC05_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">H/ACA snoRNP pseudouridylase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">GAR1_1</name>
        <name evidence="2" type="ORF">N0V83_003608</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC05"/>
    <dbReference type="OrthoDB" id="3504677at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058334">
        <property type="entry name" value="DUF8021"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26061">
        <property type="entry name" value="DUF8021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8021" evidence="1">
        <location>
            <begin position="148"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="256" mass="27734" checksum="A10DE13C07AAD54F" modified="2023-11-08" version="1">MVALLALIAPTLAVDCTRDGLLAAADSYLAAVTSGKSSDLKLSSTNFTYQQNNKVSDIAKGILSTPWKIDLNRSTADTVACASYTMFISSSGTKPYVVGTQIRHANNDTSSISMIDTVAATTGDLFFDAKKTLGYIQKEDWTIIPADQRPSRDLLKKVGDAYLDMWTDAKAADTIPWGTECERVEGSSYTSPCGKTLPHGGSAKNNGMRRYVIDEEMGSVDVLCQFDSLGPWPDSHEVRVIAGKVKYVHTITVMKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCH5</accession>
    <name>A0A9W8YCH5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Late sexual development protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003565</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCH5"/>
    <dbReference type="OrthoDB" id="5293813at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13668">
        <property type="entry name" value="Ferritin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040721500" description="Late sexual development protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="38558" checksum="8B3148BCE1411056" modified="2023-11-08" version="1">MRSALLYSTALVASITSAVPTGKRDFSPDNVYFPLPNGFPNPNAGQVLEIQKQAHGTLPNGPPPPTLSPEGITNLKLIALNELFEVAFFTELVYNLTNKVQGYDLGYGHDYVLDALSAIVAQEELHALNANGALSHFNQEPIQPCKYSFPVTDFQSAIALAGTFTDLVLGTLQDVNQLFAQGGDAGLVRAVSSVIGNEAEQEGFFRLVQKKRPSSQPFLTTATRDFAFTAIQSFTIPGSCPNINTIPLKTFKPLTVETTNIAAQTQALKFSFAKADAGLYQVDQLKLVYINGQNLPIVEPLISVNTAGDKVVFEAEFPYDEYLMNGLTIAAVTTAADSFADADAVAKATIFGPGIIEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCI6</accession>
    <name>A0A9W8YCI6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_002670</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCI6"/>
    <dbReference type="OrthoDB" id="303614at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047:SF72">
        <property type="entry name" value="OSMOSENSING HISTIDINE PROTEIN KINASE SLN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="148"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="8">
        <location>
            <begin position="503"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="418"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="457"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="625"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="423"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="457"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="635"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4373931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="646" mass="71246" checksum="0FA70579FB117C76" modified="2023-11-08" version="1">MRSTSLDSFQSSMVNSITVCGRTLLNTVEHLLDHAERKEPSETDTKKTFPGENTICITSGLPALDLSRGMSSSAPFCNIALVTEEVIETMFLGQGRFDVSLTGDDVPGALISPSSNKEIARRRTRFLIVDIADFANLDFSIPASSYARLVMNLLGNALKFTESGYILVWLRSHDGDSSHASVTLKINDSGVGMSREFLFNDAFEPFRKYNQYTAGTGVGLNVVQRIIEDVGGSIEITSEPDKGTDVTLKLPMERHIRVDDPESPHSSILNGLSQLRNRRICILHSKSPDEDGPSDNLRHWGMLKRYIDALLTTLRDELKMDVTVTSEYDGDDTTDIVICPEVSFESLQRIRDTARRKPPATVFITMDTMEADTLRCDARVMSKASVVEVMTQPCGPYKLGMVLGQCLKQYDGTNVTVLKDSDQTSGSGTDGNDSGPTSQFGPRLGLYSSKTLDLKSDFTIRGPPHTEENHWPAPSATEGEDHDPHLPENVEALGAEDVPKAQQVLIVDDNAINRRLLSIFMKKRKLLYKEANDGLQALERYKDADGKFDVILMDISMPVMDGMTSTRLIREHENNHSLKATHIIALTGLTSASARLEAWTSGVDDFLTKPVDFKQLERLMKAGRGSAGTGFLSNEESKENVDAKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCJ0</accession>
    <name>A0A9W8YCJ0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mediator of RNA polymerase II transcription subunit 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Mediator complex subunit 12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SRB8</name>
        <name evidence="14" type="ORF">N0V83_002623</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the SRB8-11 complex, which itself associates with the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="20828at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057344">
        <property type="entry name" value="ARM_SRB8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019035">
        <property type="entry name" value="Mediator_Med12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46567">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46567:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25326">
        <property type="entry name" value="ARM_SRB8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09497">
        <property type="entry name" value="Med12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01281">
        <property type="entry name" value="Med12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med12" evidence="13">
        <location>
            <begin position="223"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1222"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1302"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1236"/>
            <end position="1283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1302"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1315"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1330"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1343"/>
            <end position="1362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4373884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1420" mass="156881" checksum="D2431753A79D6365" modified="2023-11-08" version="1">MTSRPGPGIHESLQSRGSGVPPRTQVRRRPSKPANTLIQPDCIDPTLEDDRPPIHNGANDATRPPPRGRPQLFFSAVPSSGIELPIHSFPYQPTANLPAPPRPGSVHLRDALQQQRIIPGGIGVKDPPKLNAPEVPPPPVHFPGGQAADLFPWTGNSSEDTLSEALVKVGISNKSQIMNETNTARPSLWSNLKNKSGISTLSTLFVAVLEKRQQSGRLQTPNTFKPPPRLTLRDSTREQWLHDLANPTISLRRLSRTIPHGLTGKVLLEQCLNKNIPLPRALWLAKCVGINELRAHKRKGQAGTVTWGRGWTSSVEQFLDGVIGTIGQGDWKPRITYALQLATCLYKEHLLDEDHFLDWIINGLNTCPCEHLFVWLYIDLKIRTPTRLQLGADIYEGVISYLRNMSWIGNGDPNIIFKIVGELVRSKTFAAGRYLQWLIATGSLVHDTDLSSPTSWPLRLITEIPLSGLSDQIRTLRSTLLRGTPHATELEQQALEHAEDMISQSLPILFGLYNISATLPKVDVEKLNSTVRLELGIWLRKQVAQFAEVNEHVPTKDPSIEETAAVSLITASEFHIVRSWLERFGDLAILADVVNITCSSLDPSVLASAADTITFHMKTFRAIGALDPLFGRIATRYASIRTIRFPERELLLSLSNLARVAQTDGHLQQLLSYDLSRLDQRNSIAACSPASDNMGEVMQHTGSYSDDEIERILSSGNSMDQQMMARVLRKIISNQEVHVAKGQLDSDNYPTWLHRLRSFDEPTFELVINEWLDACFKGHQLDVLKIALPTLVGCGCMALSSVLDTWRVHVTQLKANPSEGMFQSALEGLHVLLPVVTLTGGCSPQDAYRYRLEQHRLCQETERRVAHCICELFELGSTTSSSIIREQLSSLASNESVLDIMRHYVVFDPGCLSKPSREASGPSLEQLLDTLLDPVDRLHLVEQTPEQQVLTVFETANELSLPICQAIIEHIFAPGTAMSGDSADALSATLLSAIKRAVDEGQSVGLELLATLDSNLTDKIRQHAEREIINASAFFASPPGIKTEEPEHTSAALVQKYLTVIDLTSSNRTETNEQAAMLIALTERFKGIAQALTDPSSLFTHPGQANNLSVPELYAWLNALLRLVVTHCETLLRTATHTHQTAFMVTMIALISHPALEFYTSISEHIFDVAVTLSDYISDDVRSHVSRLDSVKSTDDPRCLFILGVTAPVDGWLVLTKPLNPTLNQSSSQPPTPGASQTQPSPYQSPQMSTTGPATPQQRYFNQQQAQRHQQQQQMQAQQMRNYSSHNQYSVQQNKMLPAQLQRTPSNQASPSPLQQMQHMQQMQGLAQQRATQPSPVYSQQRGPTPAASQASSGGSQVSSSSGTGGGPPGKLQLQQSRQEKEMRQYPFVQPRWEILAESSGNPNANETAISLSLFGARKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCZ7</accession>
    <name>A0A9W8YCZ7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_002854</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCZ7"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08297">
        <property type="entry name" value="CAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF1">
        <property type="entry name" value="ENOYL REDUCTASE (ER) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="8">
        <location>
            <begin position="22"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4374114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="362" mass="38229" checksum="29A74ECEA350DAC9" modified="2023-11-08" version="1">MANNSNNDIPKTCKAGVVVNEGPDFKLAVEDVPVPEPGPDDVLIKLNATGICYSDIHYMLNDLPIPPMSTFGVKSPGHEGAGVVVKIGSNVKNWKVGDRAGIKPAYDACFNCELCWTGKETYCDDSPQIGLQYPGSYQQYLLSPARYTQRIPEGVDDFSAGPIMCSGSTIYCSLKESGLKPGDWAVFPGAGGGVGHMGVQLAKAMGMRVVGVDGGDDKKDLCMKLGCEAFVDFTKVKDVAEEVVKICDGKGAHGVFVTATSAAAYSSAPMMCRIGGRIMCVGLPPAGTAFAGADPLLMVGKNLHVIGTKVGSLYDTTKALEFAARGQLKPIYEVFPLAKMPEAVDKLRSGKVAGRLVVDFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDR5</accession>
    <name>A0A9W8YDR5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytochrome P450-dit2</fullName>
            <ecNumber evidence="8">1.14.14.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">DIT2</name>
        <name evidence="8" type="ORF">N0V83_002259</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.1" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDR5"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd11070">
        <property type="entry name" value="CYP56-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF223">
        <property type="entry name" value="CYTOCHROME P450-DIT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4375175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="513" mass="58559" checksum="49AC177106102791" modified="2023-11-08" version="1">MVSYLSLVFVAVFATFFAVVSFFIALFTPPRNFPTNIPTIPFYYGLLPLFKDVDQSELYRQYLKEPLAKYGAVKLFFGGQWNILVTKPSYIAEVFKHEETYAKSGNQVKIPHSVLAEYTGENIISAHGEKWKLYQGVIKPALQADQDPEQIWKNSRILRNMFLGESKKAGGNGIPIYTPLHRYALANLSEVLYSSSFETLQHMEAPLHAIQMQIKPIIFQPIFMNFPFLDYLPFKIRKLGRMLNKKFRSTLRTAITKGHTQHVCNENDKRNVACRLIGSHREGLLTDKDLDDNLVSTWYEVLAGHENPQLVLMSLMYLLGAHTEVQEAVRKEVETLFPADIPAGYEPDYADLHDLPLLTSVLYETIRMFPPISQLINRRTTAPVMLGNNIPVPRGVYVGYNAYSTNRDIEFWGADAEAFKPSRWGTTNEEINHLYRRANSKGSFISFHGGRRACLGQKFAMQQLRITLVELLRGCKWRVDESWDGRMTPAGPLYPRGLKLRFDSVNSTSEKAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDU8</accession>
    <name>A0A9W8YDU8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 21">Folylpolyglutamate synthase</fullName>
            <ecNumber evidence="6 21">6.3.2.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19 21">Folylpoly-gamma-glutamate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18 21">Tetrahydrofolylpolyglutamate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">N0V83_003027</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20 21">
            <text>(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + L-glutamate + ATP = (6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10580"/>
            <dbReference type="Rhea" id="RHEA-COMP:14738"/>
            <dbReference type="Rhea" id="RHEA-COMP:14740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:141005"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>a monovalent cation</name>
            <dbReference type="ChEBI" id="CHEBI:60242"/>
        </cofactor>
        <text evidence="21">A monovalent cation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 21">Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 21">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.17" evidence="6 21"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDU8"/>
    <dbReference type="OrthoDB" id="5212574at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.10:FF:000009">
        <property type="entry name" value="Folylpolyglutamate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.20:FF:000009">
        <property type="entry name" value="Folylpolyglutamate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023600">
        <property type="entry name" value="Folylpolyglutamate_synth_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF5">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038895">
        <property type="entry name" value="FPGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01012">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 21" id="KW-0436">Ligase</keyword>
    <keyword evidence="15 23" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 21" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="23">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00030592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00030876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRNR" id="PIRNR038895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR038895-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR038895-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ4374286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="514" mass="55669" checksum="ECE10030AA8A2C39" modified="2023-11-08" version="1">MEKRDYAAAVAALNTLQSNFSIVDAIRKSGRGMNKLAIPEMIEWCRKTGYEPSEFDRLNPIHIAGTKGKGSTSAFISSILAQYTSFADDAHRKPTKIGLYTSPHLRFVRERIQINNEPISEEQFAKYFFEVWDRLEAAAKTESAPADVPTKPVYFRYLTLMALHAYLKEGVDSVVVECGIGGEYDSTNILTKPTVTAVTSLGIDHTAMLGSTLPEIAWHKAGIFKEGSVAFTAPQKPEAIAVLQERATEKGTTLHVVDVHPALANNEIKLGLGAAFQKINASVAIAAAAAHLRVLGHSSVPDPTTTPHIELPPEFVRGLEQVRWAGRCEVRREKNVAWHIDGGHTLESIEVTGQWFAEQIASATPANTSQSKVPRILIFNQQTRDANALAKALYAALQSGVTSGAASPFTHVIFTTNQTFSEGFSPDLVSVNTNQQDVDTLAVQNALADTWSHIDSSAEVHVLRTIEEAIGTAREVARSHAKNAGADSEVMVLVTGSLHLVGGAIEVLETQKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE20</accession>
    <name>A0A9W8YE20_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Replication protein A subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RFA1</name>
        <name evidence="14" type="ORF">N0V83_002276</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">As part of the replication protein A (RPA/RP-A), a single-stranded DNA-binding heterotrimeric complex, may play an essential role in DNA replication, recombination and repair. Binds and stabilizes single-stranded DNA intermediates, preventing complementary DNA reannealing and recruiting different proteins involved in DNA metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the heterotrimeric canonical replication protein A complex (RPA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE20"/>
    <dbReference type="OrthoDB" id="1751331at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05276">
        <property type="entry name" value="p53_inducible_oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04474">
        <property type="entry name" value="RPA1_DBD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04475">
        <property type="entry name" value="RPA1_DBD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04476">
        <property type="entry name" value="RPA1_DBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04477">
        <property type="entry name" value="RPA1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000041">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000064">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000090">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000117">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047192">
        <property type="entry name" value="Euk_RPA1_DBD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014189">
        <property type="entry name" value="Quinone_OxRdtase_PIG3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007199">
        <property type="entry name" value="Rep_factor-A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031657">
        <property type="entry name" value="REPA_OB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004591">
        <property type="entry name" value="Rfa1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02824">
        <property type="entry name" value="quinone_pig3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00617">
        <property type="entry name" value="rpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106:SF18">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04057">
        <property type="entry name" value="Rep-A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16900">
        <property type="entry name" value="REPA_OB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="13">
        <location>
            <begin position="622"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="132"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4375192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="945" mass="104651" checksum="FA35D53A7EAB3CC0" modified="2023-11-08" version="1">MAEQAITQGAIKSIFEPSGCFVEHPVMQCVQIKTMEPKAGDANPVQRFRVVLSDIRNFVQTMIATTANDIVTSGQLKKGSIVRLFKYNPQRVKDKNILIIMELEVLPQYGELEKIGQPEALLDATAEPQPAAITGNNFYGSKPAPAPQQQQRSLPVHQSNPGTSSHPHLYPIESLSPYAHKWTIRARCTSKSDMREWHNQKGTGKLFSVNLLDDTGEIKATAFTEVADKLFPMFEEGVVYYISAPCRVTLAKKQFTNLPNDYELQFERDTEVEKAADQDNKPQIRFNFTKIGDLNSVEKDTTIDTVGVLKEVAEVTTITSKNTNKDFSKRELTLADDSQTSVRLTIWGKTAESFEAPLESIIAFKGVKVSDFGGRSLSLLSSGSMMVDPDIDEAHKLRGWFNAIGQNVTFNTHQNMSTSSGGAKNDAKIISQVIEEESYLQDTPSYLSIRASVVYVKNTVVAYPACSTPGCNKKVIEENPGSWWCEKCQATFPEPNYRYVLSVNVADHTGTLWLSCFDEAGQAIVGMSANEAMKIKMDDDENQTTNFMTVMQDATCKTFNFRVRAKMETYQDQPKPRYQVMNMYPLNYAQEANKLAQLIKQYDMNDDSFILKQNVDIKNGKGAIDALHIADVPKPKLGKSQALVKIKAFGLNRMDLLQREGQYPVPPGASKILGVEFSGTIAELSEEGGGKEGFKKGDEVFGLAYGGAYAEYIAVSTHMLVRKPKELSWEQCAGIPETWITATQAMYLVGHFSAGKSILWHAAASSVSISGIQLALADSASAVYATARQDSKCDFAVKELGATAAFNTTSQNWVEEVMKATDNKGVDIIVDFIGASYFQQNLDAVAKDGVIVNLGFMGGTKVPEGTDISAFIRKRCTFVGSSLRSRDEEYQGKLRDQLVEHVLPKFKEGSFKLFVEKVLPWEQIQDAHRLLEENKTKGKVICTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEG3</accession>
    <name>A0A9W8YEG3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UPF0045 protein M15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ECM15</name>
        <name evidence="3" type="ORF">N0V83_001204</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0045 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEG3"/>
    <dbReference type="OrthoDB" id="5587367at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029756">
        <property type="entry name" value="MTH1187/YkoF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002767">
        <property type="entry name" value="Thiamine_BP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051614">
        <property type="entry name" value="UPF0045_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00106">
        <property type="entry name" value="MTH1187 family thiamine-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33777">
        <property type="entry name" value="UPF0045 PROTEIN ECM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33777:SF1">
        <property type="entry name" value="UPF0045 PROTEIN ECM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01910">
        <property type="entry name" value="Thiamine_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89957">
        <property type="entry name" value="MTH1187/YkoF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine-binding protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="117" mass="12718" checksum="F81D12A35522CA67" modified="2023-11-08" version="1">MASANDTTDLKTPPSCVADFCLIPLGTPTASVSKEVAEVQRLLKKSGLSYSMHSAGTTVEGSWDDVMRVIGQCHAMLHQKGIVRIQSDIRVGSRTDKKQGFKDKVEAVERLLKEDGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEP2</accession>
    <name>A0A9W8YEP2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sphingolipid long chain base-responsive protein LSP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002127</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEP2"/>
    <dbReference type="OrthoDB" id="5599269at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036286">
        <property type="term" value="C:eisosome filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000005">
        <property type="entry name" value="Sphingolipid long chain base-responsive pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028245">
        <property type="entry name" value="PIL1/LSP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962:SF4">
        <property type="entry name" value="PRIMARY COMPONENT OF EISOSOMES (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13805">
        <property type="entry name" value="Pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="305"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="305"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="321"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="366" mass="40055" checksum="B41CD47A0C863C4F" modified="2023-11-08" version="1">MTDWMSGLATALATPVLHTSPNSPDKSRSSASLTYRVGFRNRSLSVRSKKSGSSEKTAPKHRFTMASLRGTQQPDLSKKLFKIIKSENHAIGAYEAAARERVGIAQQLSEWGEATEDETISEISDKLGVLLAEIAEQEDLYAQALEDYRGILKQIRNTESSVQPSRDHKAKVSDEIAKLKYKEPQSTKIVVLEQELVRAEAQSLVAEAQLTNITRQKFKEAYDHHFAATIERAEKQIILAKHARRLLNLVDDTPIVPGDVHPTFGEVEAARQVLTDAEDDLRNYELRVEPIQSYSGNLGVGAMPGSTAGATGTTAPESTAYEGTTRSESPSGERQYDNLGAYGGGDQVQPSYPVTEAERPNAANVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFI9</accession>
    <name>A0A9W8YFI9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase C1A papain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_001351</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFI9"/>
    <dbReference type="OrthoDB" id="2445485at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89372">
        <property type="entry name" value="Fucose-specific lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040852916" description="Peptidase C1A papain C-terminal domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="4">
        <location>
            <begin position="105"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="34"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="78"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4376070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="713" mass="79572" checksum="B7453051FFB3DE16" modified="2023-11-08" version="1">MRVSTLLVTFLQLSLSTCAIIKIRQDVPTAALNDPSATWHHHHDDDDLEHGLGLNDPSPTSDNKLDLIDRTKWMKSYDYPPVPRPGDAPWTPGPHDSKRSDGSNLPRSVDWRNRYGVNWITTVQNQGLCGSCWAFSVTALIESMIRIQHGVWSKRSEADVFEASSATCTTGWAPGWALELIKGNRFGLTDTKCNIYYDSDRPFLGCGDRQGRTLRIGDYATIESRDDARWWLANIGPVTCTITVYPDFQDWTPDQGVYRHTDTSQDPEGGHALLIVGYNDDEQTYIVKNSWGTDVGDNGYWLIGYGEVDIDDVETPKYGVTNIDPDQFARKPFRNGNIYQSAKGATHRDFEVSRTDESANILVLERQGEGQKKWAAFDNFTVYPDSGWTEQYYTPSGPVSGQPIILETSFGRQPEILYWSEETNTFNHWVYTSDSGQISAEEWVGWNQVQPGALFVNPQNDSDVYGVATGYPGFVELDTSDFSVVIRTEAGALTEYIRSTNTGTWTLTTPPIAPQGTILASGPSLVQSNINLSVYNSSSAGNLYVAAVLNSGQLALYWRTAFATSWQAGEVFGQENNIPNTPPVMIQDFWDTADENAQNGGFQLLVANYDGDVEHWQRRNDDIAGGEVPEVGVQGRWERVGVFGEGEVANVWGLTQGSFNFALEAVVEDFYGDLWHWQYTGEWERTKRLPSHYRGKSSVRTSGEGFPLFAEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFZ3</accession>
    <name>A0A9W8YFZ3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002973</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFZ3"/>
    <dbReference type="OrthoDB" id="2744543at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053144">
        <property type="entry name" value="Acetyltransferase_Butenolide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43233">
        <property type="entry name" value="FAMILY N-ACETYLTRANSFERASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G03350)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43233:SF1">
        <property type="entry name" value="FAMILY N-ACETYLTRANSFERASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G03350)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13508">
        <property type="entry name" value="Acetyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="23"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="165" mass="18460" checksum="4D44A968E290A5A6" modified="2023-11-08" version="1">MSDSEQYVILEGVPSPQVYHDLRKLAGMTPPPLEAVPKSLANSYVSLVAYERKHMLNDTTPGPEQDAVGMGRLVGDGSLFLIMVDIAVHPEHQRKGIGKRIVQRLVDYVDEHAPWAYVSLVADVPAQKLYPQYGFKDVKPSVGMFRMKRGRRLQDEVQEGAEKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGW7</accession>
    <name>A0A9W8YGW7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_001299</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGW7"/>
    <dbReference type="OrthoDB" id="265717at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052178">
        <property type="entry name" value="Sec_Metab_Biosynth_SDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618">
        <property type="entry name" value="7-ALPHA-HYDROXYSTEROID DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618:SF18">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE_REDUCTASE FAMILY (AFU_ORTHOLOGUE AFUA_5G12480)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="4">
        <location>
            <begin position="269"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4376019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="583" mass="64549" checksum="03D03101BB10C8B5" modified="2023-11-08" version="1">MADLDLSTLFHVKDLVAVVTGGGTGIGLMIAQALEANGAIVYILGRRQEKQGNIHPIKADVTSKSDLSAAVDHIAQKSGYVNLVVANSGITGPSLKGLDKGASLADYRAHLYNWDTNDFTNTYNVNSTAVFYTIVAFLELLDKGNKAGNIEQKSQVIAVSSIGAFNRVPLAGFAYGSSKAAVVHMMKQFATSLVQYDIRSNIIAPGLFPSEMTQETIKQRGEGKEAWPKDFVPEQRVGDVKDMAGAVLFLTSRAGAYINGNVLVIDENEYYEVRAIPGKGYGCFALRAIKRGTRILAEDPLLVVPMAAYMQSDIEEAFEKLSTEQKALYLSLHSGHGQDPKAWPSQIHPTVHGKERQRIQEQHDARVAKEPSLISIFQTNCMEWQKGAAIFPNAARFNHSCNPNACFTWNSGIGKETIHIMNNVSKDEEITLSYCDMTHDKSLRSWELKHYGFTCDCRACTEDEDDETTLAHQSALRRFRLQELERETRFLRGSRLDEGAKQPTLVGKLLELAALHRQEGDFTARLASIFLDLALICEFNGDLKMAEVAASKAVQVKRDCQGTDFPEYKKYEDVLVRIKVKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH37</accession>
    <name>A0A9W8YH37_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_000084</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH37"/>
    <dbReference type="OrthoDB" id="7464126at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056125">
        <property type="entry name" value="DUF7708"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF14">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24809">
        <property type="entry name" value="DUF7708"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DUF7708" evidence="2">
        <location>
            <begin position="62"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="3">
        <location>
            <begin position="264"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4377261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="509" mass="57450" checksum="DF2C57EAFF62E1DC" modified="2023-11-08" version="1">MTSVSAGARLTLQDAFERFAATVNSDDKRLFNSTNFGDVRDEAIKIERQLRARRTQRNMARLEPFLKGVNHYSSVVEALCDEATYLSWLWGPVKLMLMITVDSLGAFEKLIDAYGKIAENLPCLIRSAAALSNNHSVQNVVVLVYSDLLEFHSCVYKLVCRRAWATFFDPMWADFEPRFHNILRRLACHRDLLDRKATAGEISTAVEQNVQEVENWKHQEVEWRARKAGAVLTWLKADSASPQDTLAKHNEGTLAKHNEDCLPGTCDWFIKHTDTQSWLKDRAQKSLFWVYGKPGAGKSVICAALVHHAKANAANVFYFFCSFLDRETKNSSHVLRSLASQIIQEHHDLAIYVHDVYYQSPQVPSKKSQLALLQDLMRSLGSVRFIIDGLDEWNPSDQKELLRDLTQLLSTDPSTCICKVLVASRETLETVRGPRKGNKGVVRMSISDGSEGLAVNASIEKFIDNKLLELPEHIEDLDPDSTIMSHVKQTLIDKHHGTMALSFTSLAVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH62</accession>
    <name>A0A9W8YH62_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Protoporphyrinogen oxidase</fullName>
            <ecNumber evidence="4 11">1.3.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">HEM14</name>
        <name evidence="13" type="ORF">N0V83_001058</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>protoporphyrinogen IX + 3 O2 = protoporphyrin IX + 3 H2O2</text>
            <dbReference type="Rhea" id="RHEA:25576"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:57307"/>
            <dbReference type="EC" id="1.3.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="11">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the protoporphyrinogen/coproporphyrinogen oxidase family. Protoporphyrinogen oxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.3.4" evidence="4 11"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH62"/>
    <dbReference type="OrthoDB" id="438553at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004729">
        <property type="term" value="F:oxygen-dependent protoporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004572">
        <property type="entry name" value="Protoporphyrinogen_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050464">
        <property type="entry name" value="Zeta_carotene_desat/Oxidored"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00562">
        <property type="entry name" value="proto_IX_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923:SF3">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 11" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 11" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="12">
        <location>
            <begin position="88"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4375781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="568" mass="62439" checksum="F3C32B7CA7EEDB44" modified="2023-11-08" version="1">MRLERCAASQLLESTLRAGAFRPRVQAQCLFHLRRSSSSLLTRQRRIPPLLSATSSLVPSSQCTHQHRYTSTSTTPYPPKIAILGGGIAGLSSAYFISREFPKSKVTIFEAGKETGGWIRSKRVAVDGGDVLFELGPRTLRNATVTASLIQDLGLIDDVIFTKRSEPGAKNRFIYFPDRLNRLPAEPPSLSDLFALWRSGILGGVLGMIKEPLMPKRPDSMSDETVGSFLARRVDKRIANNIVSAVFHGIYAGDIWQLSAKTLLNTPWQLEGRYGSALGGFFRMQSEDQRPQSVSLVHPYDLDMVKATNEEIDLDPEFVKNLKEAAMFTFKDGLQHLVRALQNAVETKGNVVVTTESPIQSFTPIESEAGPGVEVVSGNEGSTQTETFDLVVSTLRNNDLTPYVTVMTVNLYFPDPKLLPVEGFGYLIPQSIPFEQNPERALGVIFDSSAIRGQDNLPAPGGGTKLTVMLGGHWWDNWESYPSEEEGLEMARAVLKRHLNITAEPLAHCVNLARDAIPQYTLGYEDRARDFADGIESEFKGRLRVVGSQVNGVGVNDCVAGAWAVARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH94</accession>
    <name>A0A9W8YH94_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001496</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH94"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058525">
        <property type="entry name" value="DUF8212"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10622">
        <property type="entry name" value="HET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10622:SF12">
        <property type="entry name" value="HET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26640">
        <property type="entry name" value="DUF8212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="22"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="DUF8212" evidence="2">
        <location>
            <begin position="281"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4376214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="588" mass="66905" checksum="5060907EDED5A033" modified="2023-11-08" version="1">MRLIDARTIELIWFNDNDVPKYAILSHTWGSDEITYQELVWINRAKALSDSSMSQVSSSTTSFGSQDEHATFMLAAMEMMIRGNSGTTMGGITEEDLMSRKGYSKIIRAAEQARGLGCDYIWVDTCCIDKSSSAELQEAINSMFRWYRDAEICIVYLEDIEKPHCNAYTSASDVARAAFEKCRWAKRGWTLQELVAPAICRFYFEDWTLMGEKTEFQDELSDATGIPIHVLEDWETVSEMSVAERMSWAAYRETTRIEDMAYSLLGLFDIHMPLLYGEGEKAFIRLQEEILKTTDDYSLFAWSAVTSNKSTYRGLLARTPLEFRHCRSIERDNIISTFPIVSTPIGLHVQLEVLPDPQDKRNVLAMIRSSNSMNQRLAISLTCLDGDMQYARVDAGSLTPIDDWPNGQLRTIYVRQKLSIPPDFATPDFRSFHISGRRANQKTPPVRIISVHPRELWDEATHELRIANDTTNNSFSVLLLRVQSPGYAGEPTFPVAFGFNRSTCHYWIRAIPDDDSFVLDKIHTRPWPETVKVRIPDEVYDARRGSDLRHHIFLVGNSGLGMSVSIRAGLVGDSIALQVHIDGLVRWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHG8</accession>
    <name>A0A9W8YHG8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Slx4p interacting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SLX1</name>
        <name evidence="11" type="ORF">N0V83_000425</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalytic subunit of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Forms a heterodimer with SLX4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the SLX1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHG8"/>
    <dbReference type="OrthoDB" id="24645at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033557">
        <property type="term" value="C:Slx1-Slx4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10455">
        <property type="entry name" value="GIY-YIG_SLX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1440.10:FF:000006">
        <property type="entry name" value="Structure-specific endonuclease subunit SLX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03100">
        <property type="entry name" value="Endonuc_su_Slx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027520">
        <property type="entry name" value="Slx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048749">
        <property type="entry name" value="SLX1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050381">
        <property type="entry name" value="SLX1_endonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20208">
        <property type="entry name" value="STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20208:SF10">
        <property type="entry name" value="STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21202">
        <property type="entry name" value="SLX1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIY-YIG" evidence="10">
        <location>
            <begin position="14"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="31"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="315"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4377598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="352" mass="40291" checksum="72E0F337B9103455" modified="2023-11-08" version="1">MADIVKLESRPIPAFYCCYLLRSKNRKSYYIGSTPNPARRLGQHNGSSKGGAKRTSMQGKRPWEMTCIVTGFPSKFAALQFEWAWQNTHATRHIERDVRDARLDELQKGKKGSSPSRRRRPSMSLEARLKNLHHLLGVGSFSRWPLHLRFFATDVFAQWERHTAKLATKLRKSIAIQLTPAELPKLAADVRVEMQTHFIPGVIRAIPVAYEDCKTYVEKSRAVLEEGRRRSCVVCKKEAVASKSLVLICPIESCQTVSHMTCLSKKFLVEEGNQDALVPIEGTCPSCRSPIRWSALMKELSLRIHGEEELKVMFKPKRRRKGEDPQDLDEQDEDLEETWMDDLNNDEERLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHS1</accession>
    <name>A0A9W8YHS1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">triacylglycerol lipase</fullName>
            <ecNumber evidence="6">3.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Autophagy-related protein 15</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">ATG15_1</name>
        <name evidence="22" type="ORF">N0V83_001753</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Lipase which is essential for lysis of subvacuolar cytoplasm to vacuole targeted bodies and intravacuolar autophagic bodies. Involved in the lysis of intravacuolar multivesicular body (MVB) vesicles. The intravacuolar membrane disintegration by ATG15 is critical to life span extension.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Binds to both phosphatidylinositol (PI) and phosphatidylinositol 3,5-bisphosphate (PIP2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome</location>
            <location evidence="3">Multivesicular body membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Prevacuolar compartment membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.3" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHS1"/>
    <dbReference type="OrthoDB" id="58570at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032585">
        <property type="term" value="C:multivesicular body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005775">
        <property type="term" value="C:vacuolar lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034496">
        <property type="term" value="P:multivesicular body membrane disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046461">
        <property type="term" value="P:neutral lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006660">
        <property type="term" value="P:phosphatidylserine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000129">
        <property type="entry name" value="Autophagy related lipase Atg15, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050805">
        <property type="entry name" value="ATG15_Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47175">
        <property type="entry name" value="LIPASE ATG15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47175:SF2">
        <property type="entry name" value="LIPASE ATG15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0072">Autophagy</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 22" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040881700" description="triacylglycerol lipase" evidence="20">
        <location>
            <begin position="30"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="21">
        <location>
            <begin position="278"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="449"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="489"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="449"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="489"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="522"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="22">
        <location>
            <position position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ4376469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="542" mass="59555" checksum="09CD0B14D5D8983C" modified="2023-11-08" version="1" fragment="single">MRRRPLCTSASRVTASLLLSFLAVSGSSALELPILPPPPASPTPSSTEKDFSLRHIFHHGTYKYPELHRRLDVPEKAAVWMAEDQHSVDREPVPRLRVKSQTMSIQRLTDRSKDTIDGILEWGRSKGRPVQLAAEDWAIDEIAGPNVTDRETVLSFARMASNAYILEPNTGEWEEVGGGFNYTEDFGWESDGLRGHIFADTENSTVVIGLKGTSPAMFDGSETTTNDKINDNLFFSCCCGQGGQYLWRQVCDCQTSAYTCNSTCLVTALREKNRYYYAAQDLYHNVTALYPDAEVWMAGHSLGGAVSSFLSLTFGHPAVTFEAVPEAMPASRLGLPVPPGHQIGSLQKRDFTGGFHFGHTADPVYMGTCNQATSVCTFGGYAMQSVCHTGQKCVYDTVKDFGWRVGIGTHKIVEVIRDVILKYDQPAKCEPYYNCTDCYTWEYFESNGTETTTTSSSKPTSTSMSRSTRTRTETCKTPGWWGCLDETTTSGKHTKTTSTSTSSSTSTCKTPGWFGCKDDTETSTTTTAKTSQHTTSPAPAPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIF2</accession>
    <name>A0A9W8YIF2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001469</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIF2"/>
    <dbReference type="OrthoDB" id="1744869at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4376188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="592" mass="64608" checksum="A7B945C3C940A062" modified="2023-11-08" version="1">MLLLVARQWQVLRGAPAVPSALCRSQRLSRPAFAATTRSYAQHSKPDEKKPWQPVPLNGAIIIPTSIPNTQPADLIHFIKPIVRERITKETFCVLFVTPAFAPWLLKDEIFLQQALARAYGELWKDSTNENPIDVHIQALCAVVDKLPAGRRFKSRATVEDEIMCRTKLPPVAEAGFEGIAYVTLPDTASVSSSGTAPSDNGAVDFIVNEGTTNDSVSNDTLRLPLANTVFQTGTRTTMMLSSWKPHHTTGRLELISKMNISHHGIRITGRDRSVDRIMSSLSIPLVPLTVPRRVEGCMGNIIRRVVGAEGKSVTASSELEEIVPQFFKSRGEPAQTTTAWALVIPRSLTDTTNSRTMRLLAKLLKKSEKGQNKRDELWGRLWRSEPPLWNNLASKAVAEGARLHRVLSGGGGWGKKAGLLSLDPVPASDAADQSSVDGFPGMLGDPEDFASTLTPVVHDGDLIQFFISPKPRIDAAGKEVNSQEKLSVLSKDNTWWGWELGTIPSTLDSMPGGSWQHTASPSEWVSVFRNSFGALAEGGLMLTRRLPGKKGDPSPAIGITMVDVPCSRFWAVELAEKVGSVEDVGEPKNVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIF7</accession>
    <name>A0A9W8YIF7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_000313</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIF7"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="28"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="348"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="414"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="421"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4377488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="601" mass="67619" checksum="960340576B9DB8CB" modified="2023-11-08" version="1">MVLTKAMDLEGLSAKRHTHFAKGEEFPLTIGDVLGHGGYGSVRKVYSPLSGRTYALKTFRRTKQNRREVESFMTELQILQRIDHHHCVELIASYTDPKTFGLIMSPVADCNLSMFYNRVTGNEDNQRILRTFFGCLAHALWFLHDSKIRHRDIKPENILVKGSEVYLADFGVSLDWANLVRSTTTEDSAKSWIYCAPEVAKLQKRNSSADIWSLGCVFIEMLTVLKGFTAHDLREKFRAHSERPRYYENLDVAMEWVADISGCGIDPEKEVIKWIHEMVVIEPSSRTTALVLSSSIAKFGKKSGDEEGVNLFCCERCYQYHESDAPSDEDEDEDEASDDDVWAEALAADTTTPATSPPATNPNRRHDASSLKLNKISRATPSTPIMPAGVESSQLSDFEEDERLDDHNRADAGLRGITDLQKPEHDDSDQSKANPPMGDSCCEVPKEADTKNALSSLTPEEPTPVAIDECINCDDSNGNPKAHSVIKGDAPLKSRYKIRLELIEKITKDRSWPFTDTRGRLPALSARSWHTPTLFLKSVQSDIPFMEYLRSADASQFERFKAAGTEDLFPIIHLLLVNGLDINHDVVKVLEQAIRERKQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIK0</accession>
    <name>A0A9W8YIK0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pyridoxamine 5'-phosphate oxidase putative domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000358</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIK0"/>
    <dbReference type="OrthoDB" id="539398at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39336:SF3">
        <property type="entry name" value="PYRIDOXAMINE PHOSPHATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39336">
        <property type="entry name" value="PYRIDOXAMINE PHOSPHATE OXIDASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G11440)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="30087" checksum="801CDD24BC7B5086" modified="2023-11-08" version="1">MGVFYETIPKSLVPWILEQKMLWVGTAPLSGSGHINISPKGGQDFGVIDERTFWYMDLTGSGVETHAHLYEPGNGRICVMFMAFDGPPRIIRIWGDARPIENGTPEFDAFVQERKVKVIPGSRSIIMINVHQCASSCGFSVPYYDFKGHRTILDDHFAKKEAKYRAGKEEESMEHYWALKSQLSIDGLPGMKTGYEFAQKKGIKPLKKMVGPYAPAAPRTAPSQVAPVYLLLVLLLGIVIGGAMTMAVVGHEGQLSASNVGVLRGLIQAGSYRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJ95</accession>
    <name>A0A9W8YJ95_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_000137</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJ95"/>
    <dbReference type="OrthoDB" id="6770063at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF189">
        <property type="entry name" value="DRUG TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G03920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4377312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="345" mass="37722" checksum="C4025902B2E454ED" modified="2023-11-08" version="1">MPLRKVYGDWKKKVAAIDFFGAFLALAGSALLVLALTWAGGEYAWSSPHVIASLITGIVVSSCFLLWQWKGTTVPLVPMEIFRSKIVLGATLTMFVNGWNFLVQVYYIPSSYQLIYGYSAVKSGALLLPVTLTQTLFSTLSGIVIHKTGRYRECLLIGWAVWAVGLGLFSTLNHHHHPSLGRQIGYALLAGFGLGNTLQPSLIAVQAGVERKHMAIVTSFRNFIRNLGGTLGLAISGTIINNAVRAKLFPMELSRLEINLLLDSPDLFRGEFGEDRTTAALSALGVAYKDGFRIVFIIGAVLNSLAFVAAWFLIPHVELKRDDDAALKEKGLRRETNQDGSIPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-06-18" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH40</accession>
    <name>A0A9W9CH40_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_010890</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3045089at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19481">
        <property type="entry name" value="RecA-like_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056599">
        <property type="entry name" value="AAA_lid_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054289">
        <property type="entry name" value="DUF7025"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054464">
        <property type="entry name" value="ULD_fung"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46411:SF2">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46411">
        <property type="entry name" value="FAMILY ATPASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23232">
        <property type="entry name" value="AAA_lid_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22942">
        <property type="entry name" value="DUF7025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22893">
        <property type="entry name" value="ULD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="987"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="312"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1191"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1262"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="456"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="120"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="225"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="357"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="377"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="402"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1197"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1279"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1294"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1309"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1341"/>
            <end position="1366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4361949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1383" mass="155367" checksum="5B672119647DBD8E" modified="2023-11-08" version="1">MSASVVPEFRAHFHPYDQLKDATTGPHLKDPAEKQREGNDKAQPPTLSAIPGMNDLLEDNTQLEVQPEQSKGEEMAGEGESKVEDDNDKSHTGSAKSGAEAPVDPDVSDHLLDATVSGREATESKEKVEDSNDHSVAGSIGALPDLTHTRKPQKTNKNKNEDKAHEDNKAFLDLRSITYLHLAQWSTRKPQYESVIEVLLDDNQDDLIPAPPPSELVVKSSTEQKVGDEKAPKTPKVDKNYGFLSPSWSKVKALQLMIHSPDLIANLKDIDLKVNWDNPTLRGRVVFIRPFKFLVLREAEIRERFKELETFRSNSVEPPPPSPLLSPDNKEVGDGSRLHEVKDRSYDSKDVDEDLGSSERSKAEEEKQSTILGSPKTRLESPSDLDHHHISDSKRLEDPTGPRQTVAGSTKTNLPEVLGTIVDDGDDDDDAEGKPAQLERLILTQKNEPLKQEAAAEATAKSVEAAQKAREDAEAKTIMEAEETKTAHEKALSEAMQAAAELDKARQIAEEELAKLRPSAAPKPPIKFKDAVGRKFSFPWHLCKTWKGMEELIKQAFLHVDLVGSHVQEGHYDLVGPDGEIILPQVWETMVQPDWAVTMHMWPMPEMPPPGTELTAMQNFRNSHIALEDFRLLIRFLDEDMKELFEMRQQIEDGTLETIAFDDLWHLFHYGEEVILEDKSSGENLRAARVFKFTGGRNTNADSTANSRKTKKIGVEALGPGSWGQAFVVQYWSLTYDGSLYWPQEGVMGIRPYEGEKEISSLPIYPSKFDRSGRIKQLVEVGNIFKQFTTELYSHRKYLGKNYETNSRGEEIDSQVIIDFREALSTEGNAYAIPDSSLPEENQINHDNRETWVEYPEHGLCGQKGWCIHEVAGPDFSLDDLELGVFLRNNKHMVHAKESIDDSEITLLPDQVPGYVLNRKYWAILSTNKNNLMEFQGTSDPWNDLVLEKKQKIIVEALVAKHLHHPHENNEIDMPYQLGDFIAGKGRSIVLLLHGPPGVGKTSTAECIAAHMSKPLYPLTSGNLPVRADDLEEQLQVHFTLAARWGCILLLDEADVFLQRRRLNELEINAMVSVFLRQLEYYRGILFLTTNRVGDIDPAFKSRIHVSLEYEPLDRRRTKKIYKLHLRRIKEALGVRRNAHGSNFKVNSEEILVWSDQHFKNASKRHRQWNGRQIHNAFQTAVALAEWEARTNPPKNPASTSNPTNAHSSSSAHDNTLPELVLGKKHFKQVAQVSEGFDQYMTDLMGGRNEAEVAAVSAQRYDYRPGKTGEPMKSGLSDSDLDDDSDSDSGGDLIGKRNETDSDSDVKQKKSKGKGKKKVFSSESSLDGGAKKTASKRKGKRKDDTSEDKSSDEVPQKKSSRKDKNKDKKKGRSKRRDSDSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH59</accession>
    <name>A0A9W9CH59_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010772</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH59"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="79" mass="8773" checksum="B17AEE09389DD9B0" modified="2023-11-08" version="1">MVSVDNLSGTATDVLYDYMEELRSIPAIKAVLDHERRRTGRSSAWSAAGMHAAMAFVEGWDEDEDEEEKEDAVEGEPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHC1</accession>
    <name>A0A9W9CHC1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">rRNA-processing protein EBP2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ebp2</name>
        <name evidence="7" type="ORF">N0V83_010415</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EBP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHC1"/>
    <dbReference type="OrthoDB" id="443772at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008610">
        <property type="entry name" value="Ebp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028">
        <property type="entry name" value="RRNA PROCESSING PROTEIN EBNA1-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028:SF0">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EBP2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05890">
        <property type="entry name" value="Ebp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="335"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="121"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="337"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="373"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4362322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="565" mass="62400" checksum="AEC1872885C52CAA" modified="2023-11-08" version="1">MARGKLKPALHQQQGKNIKLEKQRKYEKEAQKRKEKRATVTSQTDGDDKEEEGGVPVPLDEVEVKVNGKTKKSKKKGEIKPAPAVDPEWETDGSEAESGSDDDDDDMAERPALDLSRLEDSESDVSGDDDEDEDAEDGEDDEDDIPLSDLESVASEDKGDIIPHQRLTINNTAALTAALNRIQLPYSKLAFSEYQSVTSQEPVEIEDVEDDLNRELAFYKQCLSAVKDARGRLKKEGVSFSRPADYFAEMVKSDEHMGKIKQKLIDAAAGKKAAAEARKQRDLKKFGKQVQVAKMQERAKEKRDTIDKINTLKRKRQGADITATNEEDLFDVAINNDDSKSDRRGGSDGKAFNSKRQKKDQKYGFGGKKRHAKSNDAKSSSDGRDFSSRKMKGKPSGGASKRPDQAAKWGARFSSPRPKNLPQAPDGLTTPPGPLPQGTTFYLLVTKPHADTCTIHGPVRLFSHLLPTIESIVSNSPPQSTIGSFVELSDAQAAAKSKMKELLEDISEDDVQCSETWEKGGLGGGVLMAMGRMTMWEVRVLYENQALRRAKEGLDLEGGTVGWRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI56</accession>
    <name>A0A9W9CI56_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA-directed RNA polymerase II subunit RPB9-like zinc ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_009952</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI56"/>
    <dbReference type="OrthoDB" id="282270at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000008">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase II subunit RPB9-like zinc ribbon" evidence="10">
        <location>
            <begin position="22"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="119"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4363656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="160" mass="17379" checksum="6D2742E36DB4238B" modified="2023-11-08" version="1">MSSPAAQMGGTRQGGKEPILFRFCSECSNLLFPREDKSENKLLFACRTCAFTEEAPSSCVMRHEIASTVGATAGVTAEVAQDPTVGPSHSDSQASLSEEMPCCTMCGMPIMCEECGEESAPGFALEAEDEPSQPPTDPQEEEYEDYDDDYDEDMDDEDMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CII2</accession>
    <name>A0A9W9CII2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009629</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CII2"/>
    <dbReference type="OrthoDB" id="3778423at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="335"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="451" mass="49445" checksum="19A7EA63CD09CDD8" modified="2023-11-08" version="1">MSDHDWHDLRETATGAMNIVRGGIPSPVLASLIDEKVPTGFARPCRPKPATSALLHKELAETNGLYFQNIAEAASATAQALWRPLTNDASIPVSDEEDRAVVKTLVDAFKDMTIARDTPGNAYRKRLTKGEAVFYNEWSIEACAWNILGMLKSIHTKGFNAPIYDHGMVDSIGQTQLWTFQERLDWVTLVLKKHEKLWTAIGAPHKLYNSTLVNFASNANRNNWVKAGREADEAHTNRPKRQRTVRPFDTNMSTFSSTTTTKDRASTRAGRPRKATTKEQVTSKDKKETKEAKELDGMVSSQLSPVPSLCTLTCSFQAAPADANTAEHEPADIVSTSSATATTTSSTSTSAPLPQQITADVEMTDIDAALLAEKKLTEKNFEDSMVATERKVSEQEHIVTGTSFTSLQANVPTATAPQTHVTAHRAVDDVQLHDAQTMEAAYLLGLLHTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CII7</accession>
    <name>A0A9W9CII7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009644</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CII7"/>
    <dbReference type="OrthoDB" id="550558at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00838">
        <property type="entry name" value="MPP_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052963">
        <property type="entry name" value="Pantetheine_PDE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36492">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36492:SF2">
        <property type="entry name" value="[ACYL-CARRIER-PROTEIN] PHOSPHODIESTERASE PPTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="25"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="319" mass="36815" checksum="85136FC49CDD9540" modified="2023-11-08" version="1">MHNASSATTETTPTPPYTPPTRPGRLWAISDIHLSFKANREALEKLLPHPNDDLILCGDVGESAEHCRIAFAKATECFRQVFWVPGNHELYTLPSQKEQGCRGEDKYMECVEVAREFGILTPEDEYTLWEGEGGPCLIAPIFTLYDYSFRPDHVKLEDALEWAREEQIEATDEHLLHPDPYTSRIEWCNALVEKTERKLSAAVTAHPNIELIIVGHWPLREDLVKLFRVPRFSLWCGTKKTEDWHNKYNAKIVISGHLHIRRTDWIDGTRFEEVSLGYPRQWQECQDSGLDINDLLREILPGPETPPEGERNTVWRRYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ11</accession>
    <name>A0A9W9CJ11_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphoribulokinase/uridine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_008599</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the GLYK kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4364983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ11"/>
    <dbReference type="OrthoDB" id="347435at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001691">
        <property type="entry name" value="Probable ATP-dependent kinase TDA10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="10">
        <location>
            <begin position="31"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4364983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="297" mass="34027" checksum="8AF21622F16F7F05" modified="2023-11-08" version="1">MTTLESNIRRALDILLPKIKERHQQSSAPIILGITGLQGSGKSTWASRIVDILSSEHQLYTITISLDDLYKTHDDLIAQRDKDPENKLYRTRGQPGTHDEQLAGTFFKDLKAYTGEGELKIPSFDKSKFKGEGDRAPEPDWPTITKKPDVVVFEGWCVGFQPLPAASIEEKHNLALSGKLPVNTPAQHELAHVLEVNENLKRYCDVFMGPQHFDFFIHIDTDNLRNVYTWRLQQEHKMIEVKGTGMTDDQVRAFVDGYMPSYEMYLDKLRDGLFEDKGRLVKVVLTKEREIERVEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJY9</accession>
    <name>A0A9W9CJY9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007937</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJY9"/>
    <dbReference type="OrthoDB" id="5420410at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031349">
        <property type="entry name" value="Tfb6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37781:SF1">
        <property type="entry name" value="ADR380WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37781">
        <property type="entry name" value="TFIIH COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17110">
        <property type="entry name" value="TFB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="302" mass="32497" checksum="AED87B5BF3509BDD" modified="2023-11-08" version="1">MASITSPTSAAFAETLPTPPASSAAPSQHASPLPQPRRHPLKPGGLKESELIRYLDHSVNHIQKRVDNRITNRKVKPAAGEEDGYSAFWEVAKDLDGLVDVVWVSGSPNLEIPYLLRLAVLAVEYLPMFPTSTRSAQVTFSLISKLDYAFSSLLTGHDTASGEPLPGFESGRKVSTTDKVRLKGIVDRTRLTVVRVMSGASEVGDEGDVGGPMDTESDGEGEAGGRNRTVTFEGFEDNLEDEEDEDWEERNVASVYEKTIGELGDVLGGPPIGIITDDWGINGTAQQRSGQGFVESGGEIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK57</accession>
    <name>A0A9W9CK57_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_007614</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK57"/>
    <dbReference type="OrthoDB" id="10020554at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043337">
        <property type="term" value="F:cardiolipin synthase (CMP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000017">
        <property type="entry name" value="Phosphatidyl synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050324">
        <property type="entry name" value="CDP-alcohol_PTase-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269:SF60">
        <property type="entry name" value="CARDIOLIPIN SYNTHASE (CMP-FORMING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4367084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="296" mass="32424" checksum="FF86E94C51C0EB76" modified="2023-11-08" version="1">MITQQPPISTTAKLATTLKSTLKLDTVRHENIYNLPNILTLSRLIATPAIGYLIIHNHHLYAFSLFAYAAISDLLDGWIARRWNLQTVVGSVVDPMADKLLMTTLVTCLAVNGSLPMPLFALILGRDASLAISALYYRYASLPAPKTMARYWDFSLPSAEVHPTTISKFNTFLQLSLLGTLLCTNLLHDPSATSSAAGGLLMSIQELLGGEEGVKTMVQGMSAIVASTTIYSGLEYTWSKKAVVILGGDEALKRKQGFRGRMIMASGFGSFIALAVYLWYKGQTEEEVEKEVKQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKA5</accession>
    <name>A0A9W9CKA5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKA5"/>
    <dbReference type="OrthoDB" id="3781461at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="86" mass="9189" checksum="1B3EFC0E6BEAE184" modified="2023-11-08" version="1">MPRGAYDLTDPAAYKSSAAAEAITMHQRQVKQEQAIAAAEQAATKDQKLIKSKKSRGIVDARKATKEAAKTDGQASEAHNEAPLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKR4</accession>
    <name>A0A9W9CKR4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007007</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKR4"/>
    <dbReference type="OrthoDB" id="3783802at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="169"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="212" mass="23024" checksum="81323E9BE48021DF" modified="2023-11-08" version="1">MAPTPTTEGLVADPTSSATPDSGYWTKTNIAVTTVFALLALGIFLALLLFFLHRRAQKKKKHATHADKAGLLANEDKTSMFNRNRESSVTLYVDSEADAQSKRVSQDTISLIPLQITPVEERRDPMNNTTESTGSGISSMSRSTNANTLSTILLSPVSPTGDGGDLGIRPSGRARSTSTSSQRARYYETTPQNIEMPPIPKIIRTPSDDEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKR6</accession>
    <name>A0A9W9CKR6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Multidrug resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CDR1_5</name>
        <name evidence="12" type="ORF">N0V83_007128</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003632">
        <property type="entry name" value="ABC multidrug transporter (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="497"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="529"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="606"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="638"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="743"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1163"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1194"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1238"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1282"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1315"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1437"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="134"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="824"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="16"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="788"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="803"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4367544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1466" mass="164570" checksum="C113C0226D151583" modified="2023-11-08" version="1">MSAAVGFTEVAVHTEKPLANHAKTESSSDSGLEMKDQIRRDTESEREVVDLVRKFTTQAQQQHLGSPFAAEQNSRLDPKSERFRARDWAKAFYDIRYSSEESSSRVAGVAFRGLNVWGKGSPTDFQSTVGNKISKLPYLFGRGVQKIEILRDLEGLILPGEQLCVLGPPGSGCSTLLKTIAGETHGFKVSPDAYLNYQGIPATDMKKSFRGEAIYTAEVDAHFPQLSVGDTLYFAARARAPHIIPGGVSKERYAEHLRDVVMAMFGISHTIDTKVGNDFIRGVSGGERKRVTIAEAALSFAPLQCWDNSTRGLDSANAVEFCRTLRTQCDVFGASTCVAIYQAPQAAHEVFNKVIVLYEGRQIYFGLAHEAREYFERLGFECPDSQTTPDFLTSMTSASERRVRHGFENITPRTSDEFARCWRESPERQQLLLAIDEYHQAHPLQGEDHKLFALSRKAEKSRRQRQRSPYTLSYWKQISLCMWRDVQRLKNDPSVTLSMLIINFFEALIVASIFYNLPSTTESFFKRGGVLFMMVLLNAFGSILEVMSLYSKRTIVEKHNRYALYHPSAEALASMIVDIPYKIANCIIVNSTLYFMANLRREPGPFFFFLLVTFTMGLSMSMLFRLFASMTKTIAQALAPSSIVLMILVLYTGFVIPVQYMRGWASWLRWINPVSYGFGNVMVNEFNGRTFDCAFFIPSGPSYENVSADQRACAMQGSVPGSTSVSGTAYVETAFSYEYSDRWRNYAIILAITIILFVAHLVTSELVASERSKGEVLVFRRSKMQVNSKRHGGDEEAGSETAHQGERFDKSDSEHLVQKQVSNFHWEGVSYEIQIKGECRTILDSVDGWIKPGTLTALMGVSGAGKTTLLDVLASRTTMGLISGNMLVDGRERNESFQRKTGYVMQQDIHLETSTVREALEFSALLRQPPEYKRKERLAYVDHVIRLLDMEQYADAVVGVPGSGLNVEQRKRLTIGVELAARPKLLLFLDEPTSGLDSQTSWSICDLMEKLTRNGQAVLCTVHQPSSLLFQRFDRLLLLAKGGKTVYFGDIGHGSRVLLDYFARNGAPECPAGTNPAEYMLEAIGAAPGAETNIDWPSAWKNSKEYTDVQAELASLRELATQPSAVMDSSDASHQEFAAHFMAQLKTVAQRCAQQYWRTPSYIYSKVILTVGCSLLIGFSFFNGENTMQGLQNQMFGVFIFLFVIIQLLYQILPMFISQRTLYEARERQSKTYAWQAFVLSNLVIEMAWNAFMAILCFLVWYYPAAVYRNAEATNSTHIRAFHALLIVITSFLFASTLAHLLIAGAPSEEIAGAYATLVGIMLYGFCGILAGPSQLPRFWIFMYRVNPVTYMVSSFMSTTLGQAPAYCAETEFQTFAAPANQTCGEYMQNYIEMAGGYLRDAQATGQCQFCQMDNTNQFLQRIHANWDTRWRDFGLLWVYVVFNAAGAVFLYWLCRVPKGKKTKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL12</accession>
    <name>A0A9W9CL12_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_006756</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL12"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000042">
        <property type="entry name" value="Similar to amino acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF402">
        <property type="entry name" value="AMINO ACID TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="418"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="443"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="473"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="499"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4368399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="527" mass="56439" checksum="BD991A371BCC5F88" modified="2023-11-08" version="1">MAPSETTPLLQHQPDQTPHAPDEDEELPNNPPRGTFARNLSARDGFALLISIVIGSGVFSSPGPIDANVPSPGSSLLIWLLGGILAWTGALTMAELGTAFPGEGGIQPYLSHMYGPFWGYMAGWSWIVATMPATLAILSIVFVESVYSSLGVSEPSPPVTHKLLSLLVLVCVSTANSISTKTSTRLSGFFVAVKLLTLLLLVVAGLVVVFVFIGSSRGKDLGGGDWHQCNWFAPRSTALPDGRGGRLNWRDVGLWESLGFYSTALYGALWAYSGWDKANYVAAELKEPSRQLPLSINTAIPTIVACYVGANAVYYVLLPWDKVATTDAAAVTAVTHFLGKGAGYFATALICLVIAGSALGNSFVAGRMTVAAANKKWFPRVFGTVGQIGTFKVSKAAQEETEGADDAMDKGESPINALILNTALSAIYILLGNMRILLTLNGLAEYAFFFLTVLGAIILRYREPNLHRPVKPIILIPLLFAVISGFVLVRGAVFAPVQAGILAGIWVLGAIFFYVRMRFLRRRVESP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL83</accession>
    <name>A0A9W9CL83_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF1753-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006548</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL83"/>
    <dbReference type="OrthoDB" id="3338076at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070916">
        <property type="term" value="C:inositol phosphoceramide synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070917">
        <property type="term" value="F:inositol phosphoceramide synthase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006673">
        <property type="term" value="P:inositol phosphoceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013862">
        <property type="entry name" value="Kei1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28077">
        <property type="entry name" value="INOSITOL PHOSPHORYLCERAMIDE SYNTHASE REGULATORY SUBUNIT KEI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28077:SF1">
        <property type="entry name" value="INOSITOL PHOSPHORYLCERAMIDE SYNTHASE REGULATORY SUBUNIT KEI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08552">
        <property type="entry name" value="Kei1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="325" mass="35588" checksum="FF33148FE4C98368" modified="2023-11-08" version="1">MITPSHILEIPRPKTFLRTMSLRTGAEIITFLQVINKASGVYGLLALLTGAYIDGWQLSMYLYSTVALLATVYLYKHIRLQSPFESVLLAHLYALDSVINALYTAFFGIAWFYTLASHPDEDSTLSPGLSDNAGFTSPQHNVSHVDIVAEATEGMQAGQNAVTIGQGTGNAGAGLSNAVFQSGSIMSISLISGFWALRVYFVFIMLAFARQCLRQHIAANASSAAWYNSNNMQTTANDLAENPFLEGKEEGNGWKGKLGRVMLSGAPKYWLGADEGEDWMRGVGGKFRKTTQLEQPLGFSERERRRRSGTGPPVPQLNLNPELPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLF5</accession>
    <name>A0A9W9CLF5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Prolyl 4-hydroxylase alpha subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_006774</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLF5"/>
    <dbReference type="OrthoDB" id="420380at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF242">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Prolyl 4-hydroxylase alpha subunit" evidence="7">
        <location>
            <begin position="74"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="304" mass="33879" checksum="E0DBADD3A993A83D" modified="2023-11-08" version="1">MAKTDNNSATQLKKATSWTTLHLTLFTLLGALTAIVLTPYALSSPGHPAASYAPGNSTLDTLANPKIKIISFDPFIAHISDFVSKDEREYLLKLGNPLLKKSIIKNDDGSEVISEARTSSTAFLPEEDPVVLSIAQRAADFQGYLSPGDIDVQITSYNPGQQYKHHYDWYTPPRTRNRVSTFFAILKSTCENCGTEFPFINRTERAQFDGRWCDVIDCEKELLTTKNVEGSALFWLNLDGNGEGRIDMLHAGLPAVNGDKIGLNVWTDIDLQEMKRRGLFGGQSRHYFKPTKEFLESHGLMKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLW7</accession>
    <name>A0A9W9CLW7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005702</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLW7"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="134" mass="15311" checksum="2F62992E3CBDFF11" modified="2023-11-08" version="1">MGYQPRANGSDEDDDDDNEGDDSGHDLRADPASSISTTVQHTWSYSSACSAITALEQRFRFRWNNFELSYQQRQRATGAIDALLKAGYITKSERIPLQQTAARCQRSCDVATKASFRYVDLRLRRCCRRHPHYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMN8</accession>
    <name>A0A9W9CMN8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003780</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMN8"/>
    <dbReference type="OrthoDB" id="5342184at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021986">
        <property type="entry name" value="Spherulin4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35040:SF7">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12138">
        <property type="entry name" value="Spherulin4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4372007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="221" mass="25342" checksum="AE81FB71429B3709" modified="2023-11-08" version="1">MGSLPDQVYLDEIPKLRAYPNIRTLGYVATHYAEKDITSVLAEIDVYARWPKTTNNTKMRVDGIFFDETPSTYDADDYAYLKKAGQAVKNGTTFKDHFIAHNPGLVSPQILNSKTVYQQSYLNLTDITVVFEETFDKWLDKDNFDPLQKQKIRRSKLAVILHSLPNVSKPVLDFVIEQVGEAADWLFLTDVKDTDMYYHSFSPMFADMVKGVDGGRRRKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMW5</accession>
    <name>A0A9W9CMW5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Calcium uniporter protein, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">N0V83_004406</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Highly selective calcium channel localized to the inner mitochondrial membrane, which mediates calcium uptake into the mitochondrial matrix. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates ATP production, cytoplasmic calcium signals and activation of cell death pathways. Sufficient to operate as a pore-forming channel without the need of calcium-sensor or auxiliary subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homotetramer, assembles in a dimer or dimers configuration with two interfaces.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCU (TC 1.A.77) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMW5"/>
    <dbReference type="OrthoDB" id="278338at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990246">
        <property type="term" value="C:uniplex complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015292">
        <property type="term" value="F:uniporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036444">
        <property type="term" value="P:calcium import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006769">
        <property type="entry name" value="MCU_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039055">
        <property type="entry name" value="MCU_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13462">
        <property type="entry name" value="CALCIUM UNIPORTER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13462:SF10">
        <property type="entry name" value="CALCIUM UNIPORTER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04678">
        <property type="entry name" value="MCU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="406"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Calcium uniporter protein C-terminal" evidence="20">
        <location>
            <begin position="340"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="195"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="497"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="203"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="236"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="521"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ4371190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="530" mass="60345" checksum="A6B1A14093D15EA2" modified="2023-11-08" version="1">MKHRLSALASLSRGATLRTAPASQLLGTYSRIAYPTRPAAAARPLQLRCFTITSHPRSESFRSNGYNNSNARPTLELNENVTQEEKDHFAKKLDEDKGKQIRTPWHREGSDKPPVARQRSAGAMTKGKLLTTPSRMLKIILPLTTRDTNTDRKDIEPLALLVHPQQPISYLERLIQAELPTIKDKDGKERIPNVHFRAEDSMQGDSQPSEAAETTVSRDSQSVARQDGQQDFENVDELRIDGKTEKTGKLSGSSKDRKTPEEAEALRGAPGQGGVESYSGQGQEAPSDMKAKRKFVRWSSSTEIGDFIRDAARGQEFAIEIEGAPEEIRVGVPSFNERTYYLRMRLRKTSRKISEMADVKKECDELAHKAAKNVARAGFAGIVTWWGVVYYLTFQTELGWDVMEPVTYLVGLSTLICGYVWFLYHNREVSYRSAMNFTISRRQQKLYQQHNFDLRKWETLIDDGNSLRKEIKAVANEYDVEWDELREEKDEKVVEALKDERKKRKEGNKNKDKEDDEQEESKDRAETKGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNV9</accession>
    <name>A0A9W9CNV9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_003688</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNV9"/>
    <dbReference type="OrthoDB" id="5329176at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF123">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="4"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="142"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4373393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="193" mass="21732" checksum="697670F2CA21BEE9" modified="2023-11-08" version="1">MKTTSAFHIAMDLWILVLPLKLILRIPRPPREKLALIIIFSLGIISTIASIIRLQSLRIFTLSNDPFYDSLPINTWSMVEVNIGILCASIPTLKPLVSKAQRHRTKNALLKYSSKKDINGEWPGDSKVAILRTGKDMMISLHPSTMKGGDTKSMDDEEWELDGRPPPVPPKDDVFDIIQDPPPRYPDMAYGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNY2</accession>
    <name>A0A9W9CNY2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_002705</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNY2"/>
    <dbReference type="OrthoDB" id="167718at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12400">
        <property type="entry name" value="RRM_Nop6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000376">
        <property type="entry name" value="Putative RNA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034228">
        <property type="entry name" value="Nop6_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF51">
        <property type="entry name" value="NUCLEOLAR PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="265"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="344"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="118"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="355"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="376"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4373966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="418" mass="46616" checksum="88356F3E8FDFB60C" modified="2023-11-08" version="1">MSKTKEEKKRKRDVEATEDVPKKSKRSKKTEDVLDAPATEAPVVEAKKEKKDKKDKKAKKVKDAEAEADIEEAPKEKKEKKSKSKKSKKADDVTESAPADEVELIHDMKATENFIGVTDDDEPMPDAPEETESKASKKDKKSKKDKDSKKDKKSKKSKTSTESETVSTGEAEEQNGVSEEAVADTNGADTEAKVDKKEKKKSKKDKEAKKAKKEKKSAKSETNGDVPTEEQPVVETNGEAEAEEQNAEEDAEEGETEGEGKKGRFIVFVGNLPYSATKEQIEEHFAKIKPTEIRLRTYKGTNKFMGTCFVEFDRFDRMETCLKKYHHSLFPDGKKKSEGRKINVELSAGGGGNSEARKTKIMKQAERKDKKASKQKKGEAEPAAGEPEKAAEPDNGYINPARLAMMSRPAPPTHRQRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNZ3</accession>
    <name>A0A9W9CNZ3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription elongation factor spt5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SPT5_2</name>
        <name evidence="2" type="ORF">N0V83_002735</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNZ3"/>
    <dbReference type="OrthoDB" id="10257049at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051397">
        <property type="entry name" value="Zn-ADH-like_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677:SF4">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="1">
        <location>
            <begin position="28"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="286" mass="31109" checksum="DE3CE79291E0167D" modified="2023-11-08" version="1">MKAIQITKFVTVSFSTPLLRTKLTRTIQNLKDLTPRDVPDPKHKGPHDVHIKITHAAVTHVDLLYAQGLHQNNKRHVKPPFILGNEFSGIVVSAPPHSSFKPGDKVWGGGLGSYAEQICVPEESLHHVPAQWTNAEACAVGSSGAVSYGALISVARLKAGETALILGASGGLGVMAVQIAKAVGAKVIAVVGDDEKAQMIREIGADAVRFGEDGRKDPQRTKDVWDGFMKLVETGKIRPVVYKEEYWGLEAVPRALEDARRHKSWGRAILRINEDAERETGTKPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP85</accession>
    <name>A0A9W9CP85_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8004 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002901</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP85"/>
    <dbReference type="OrthoDB" id="5302380at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058317">
        <property type="entry name" value="DUF8004"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39601">
        <property type="entry name" value="CHORIOGENIN HMINOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39601:SF2">
        <property type="entry name" value="CHORIOGENIN HMINOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26013">
        <property type="entry name" value="DUF8004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8004" evidence="2">
        <location>
            <begin position="411"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="820"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="882"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="255"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="826"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="882"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="905"/>
            <end position="918"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1081" mass="121559" checksum="397D6DB631DD5F70" modified="2023-11-08" version="1">MSARGARSRKVQQKDIQKKEKKTTGKGKAPDAIYELPHDAFADWRPTPSLNDPRNGVSRSITVSSNTSRATSRSGGTNDSSGRPRLNTHHTNGSKELRASRAPQSSGGSASVSDLSSKGKDKTTFQAHGKETKAPRGRGGGVPIKRSVTPAPTLPVFRTESAASYPSTNQKTWMNFRVWEGGKDGMRPYGGFEHDEDMQNGSVLIYFMEEHADEDRPNPSIRAELDVLENSGSTWLNQLLLYGRVDDDVDEWDLPNSPSSPIPSQNFSPNFPQSEQRRMLGPTSPGGMSPPPFDIDRTYSRIDSRAVSRLQHFSEITYSDGSDSPPPGRQTSRRHVTHEIRFNAPEDVKTAQGRRLHYVAIRNSLAMLHSKPIVGADVFDMLSTLQPQIQVMYDLDSDDYSRMTPRERSVQMVTHYLGQHGLDDVRKSIRQALGLLAWSEQDSVRWRQGYLESFVHLAGVMTPQVEDLAEFKRLSVVTRRNLGLTAKTLQLRVMEAEERLAGFDFSDLWEDNVRAASTAVYQSYQSFRQFLVNHYTRIYGNWPPTSNNTWLNRKVVLAMQEDFGSLYDYLVNRDVVWNPREERASRKWEMAHRKNDGFRADLPELGITDMLVIFDNKYGYAHIPHPYPLLPREVPQASREKEKKSLFDRMKKDKGKGATKDAKAHLQLSIVFSDATNIEKLDVNFNGSTLIDKFEQFELTTDLKTATPREARLGRWVLLYSILQVLSTLSVDVQGLKHTDGVRYFLCTDLKRCPEWVTNGQMEYLEASQHRSWCWQRSWDPVPTQTAPIELEASTTMNSRNGAANPYERELSAEMVGHTHVAHGLPSPPPERSLPAPPPSFDGATLLQNDIRRLGEKIDNYSLSHNATNIFRQEFERRRENEKVMQGEFHDRKPRLEGETFGPRAPTNTPNNNNNAANMTSFRPRESSLPQQQHQQQQQFSNERSQSRLLLDDFRDSRQGALLPPSASALRSHVTSSVNTDLGGYPFSSPSNNDMQWPVPPGYDDDAHASNRESTVLGSEYGGGGGFSDSESRYVDGRARAMGLGGGEMQRGRGGCGRGVGGISGDEESKGLFILVFILWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPC0</accession>
    <name>A0A9W9CPC0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7 11">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_003478</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 10 11">Belongs to the peptidase C12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7 11"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPC0"/>
    <dbReference type="OrthoDB" id="1924260at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09617">
        <property type="entry name" value="Peptidase_C12_UCH37_BAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.532.10:FF:000009">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.532.10">
        <property type="entry name" value="Peptidase C12, ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001578">
        <property type="entry name" value="Peptidase_C12_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036959">
        <property type="entry name" value="Peptidase_C12_UCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017390">
        <property type="entry name" value="Ubiquitinyl_hydrolase_UCH37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041507">
        <property type="entry name" value="UCH_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589:SF16">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE ISOZYME L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01088">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18031">
        <property type="entry name" value="UCH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038120">
        <property type="entry name" value="Ubiquitinyl_hydrolase_UCH37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00707">
        <property type="entry name" value="UBCTHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52048">
        <property type="entry name" value="UCH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7 10" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 10" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UCH catalytic" evidence="12">
        <location>
            <begin position="4"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8 10">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8 10">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="site" description="Important for enzyme activity" evidence="9 10">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR038120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR038120-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038120-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4373186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="311" mass="35106" checksum="E730077D4EEB9412" modified="2023-11-08" version="1">MSGGWNTIESDAGVFTYLIEKLGVKDVQFEELTALDSDEIQSLGTTYGVIFLFKYPTGEAPSDKPKDGTYDHDAANNLFFAAQTIQNACGTQAIISLLLNREGDVNIGKDLKEFKEFAGEFPPELRGETLSNSDLIRETHNSFARSSPFIDETQRTATEDDDVYHFIAYTSINSTLYELDGLQPAPISHGPCTPSDFHTKIIPVLQRRIARYPESEIRFNLMACVKDLRIRAREIGDEDGLEQQEDKRMQWLWENSLRRHNFVGFVGELLKGVVKAKLEAGGGAYDKWVEEAKGRTKKRREEARKQGIVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPC9</accession>
    <name>A0A9W9CPC9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003358</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPC9"/>
    <dbReference type="OrthoDB" id="20872at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42345:SF2">
        <property type="entry name" value="HELICASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42345">
        <property type="entry name" value="TPR_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4373067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="482" mass="53859" checksum="5BA1561B147E5A10" modified="2023-11-08" version="1">MHFGFGPSGTRQRRIDSFCLMLTRAFYDEPTRFTDFTKARTFTNNFLTAVNQETKTRDFLYQVLLGYELLIRLKLQPALTSYAGIMTDYISALIVTADLFMQNVQLTTPTAITATTSLTTTNPPRYAFFAINHQRNAEGLIRIAEALSWPLMDETRRTLETAYFDLTSGVSGASYDMYDWLFGLVMPGRYSRHRVMCTLVDATPSIRNWQGAPYYDNAVVVKNKSYWPKRTVLGRVLGGLRNPKSVCGWIGPLPAPTGTDKNGGAIQGWVSLNARRLDVPVPIIRLAKPLEALGFTDTDQTTNEQIITEIVDANEYIISSGPVVPPGHQKCVFKGIHLELIPQARLNIGQTLGLPTEEYRASLDFEISSQSVRYALFTLPIFVTAPPCVGTHVMFRRQAQMRLRDAFLVKDLKDTYPVPDKMLVINAMGEGDEIVARAWCAERGKHAVIRRDVPGKECCFACACDLAAGDTGLNCNVLIWAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPN6</accession>
    <name>A0A9W9CPN6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hsp70 protein that interacts with Zuo1p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">SSZ1</name>
        <name evidence="4" type="ORF">N0V83_002602</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPN6"/>
    <dbReference type="OrthoDB" id="29851at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10232">
        <property type="entry name" value="ASKHA_NBD_HSP70_ScSsz1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000009">
        <property type="entry name" value="Heat shock protein Hsp70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000023">
        <property type="entry name" value="Hsp70 chaperone (BiP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF32">
        <property type="entry name" value="HEAT SHOCK PROTEIN PDR13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="473"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="473"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="489"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4373863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="573" mass="61125" checksum="BFD7BC0F5312605A" modified="2023-11-08" version="1">MSAQADPAQRVAIGISFGNSYSSIAFTSGDGKAEVIANEDGDRQIPSALSYVEGEELHGGQAKSQFVRNPKNTTAYFRDFLGQDFKSIDPSPCHQSAHPIQQGDGVAFSIRDTDGEQENTVTVGDITTRHLKRLRSSASDYLGKDVNAAVITVPTNFSDAQKEALKKASTDAGIEVLQFISEPTAAVLAYDARPGAQLADKIVVVADLGGTRSDVAVVASRGGIYTILATLHDYEVSGFKLDQVLIDHFAKEFIKKNKSAGDPREDDRALAKLKLECEAVKKALSIGSSANFSVESLLGGIDFTATINRTRYDLLGNKLFAAFTRLVTHAVEKAHLDPLDISEIILAGGNSHTPKVASAVRSAFAESTTILAPSTSPTAINPSELAVRGAAIQASLIQEFDLEDINQSTHPMVTVTPHLSTAVGVLCISGDDASGIFHAIVDAETPLPVRRTATIATPREGGDVLIKLAEGSRHIKITKPEPKPKPEKEEDTADEDEESDDDDSEEEEELREKTWKVGKTLSEAAIRGVKKGGKIEVQINIGADLSINAIFRELGAKGGIRGIIQGQKTNGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPW6</accession>
    <name>A0A9W9CPW6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002827</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PHAF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPW6"/>
    <dbReference type="OrthoDB" id="411211at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005373">
        <property type="entry name" value="PHAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039156">
        <property type="entry name" value="PHAF1/BROMI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13465:SF2">
        <property type="entry name" value="PHAGOSOME ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13465">
        <property type="entry name" value="UPF0183 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03676">
        <property type="entry name" value="PHAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="275"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="309"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="438" mass="48426" checksum="7DD842406D167328" modified="2023-11-08" version="1">MEDGNMLLQPGRSVGFMTLGCSLHEVLTTVKAEVKAYPRFQLYHDDLHPISTPIQIVLPDNGLRLQFDGPDQRLRLIEVLDFVKAKLAYKDIEVFKPGDGGTLGGPSGPRFRHIYDKLLGPTYGGEYVPPKSDDPNARGTYNLSYPGIAFNFPVQHSAYSSKKDFISLLSSSATGPATSMAVFSGESWQKARGTLFTAIPPNPRSLALVGGKGREGGPDEIDLVKVHGEGRIELTRRTSPPFWIILSETTPQDLIMELGAPDSIYRKNDHRLSIHKDRRTSDASDLSPGGLTPDDLDSDHGSAIRSDNDDAADDEDWEDSSSAALEAQEREVAAAEHFYNYYHHGFDILISQPTQISPPSPTAERKESDLYPTPTAAQPLNHLTATKIIFHGNVPGSYQFNRHRRSRWTLEHVPSTTYRDPSTPRCNSGIFREVKGCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ87</accession>
    <name>A0A9W9CQ87_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_001843</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ87"/>
    <dbReference type="OrthoDB" id="409395at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031380">
        <property type="term" value="C:nuclear RNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031048">
        <property type="term" value="P:regulatory ncRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001366">
        <property type="entry name" value="ATP binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002468">
        <property type="entry name" value="Suppressor of ascus dominance 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057373">
        <property type="entry name" value="ZNFX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF341">
        <property type="entry name" value="NFX1-TYPE ZINC FINGER-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25396">
        <property type="entry name" value="ZNFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZNFX1" evidence="5">
        <location>
            <begin position="149"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="3">
        <location>
            <begin position="335"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="4">
        <location>
            <begin position="725"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1029"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1035"/>
            <end position="1048"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4374767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1110" mass="125483" checksum="EC7C525E4B02322E" modified="2023-11-08" version="1">MNLAHHTTDGEVNSHLSERFSGASFFSSGLIPDDNVPNSGPSRANNDVREYFELSKKPVKGGAWLDQPEIPSPSEILREKTGSSSTEQADALIELDEKIRPHKVDGAYESNEDYLRTKYELLREDAVRPLREAIDEIRADPFKDEAEYKDQSIGIYDPVYLTSLVFSPRGIATRVAFSLARVKKRIRWEQSKRLITGTLVALSPTDDAFQTQCVLATVAARPLSALEQSPPEIDLFFARPEEQEIDPMRKWIMVECRSGFYEASRHTLLALQHLMREPFPMSKYLVHAQKKVDLPAYIEHNPNINLSSLVSMEESADFENVNILEEWPSASSHSLDKSQSKALKRMLTSELAIIQGPPGTGKTFVSVVGIQIALENLRKDDAPIIVTAQTNHAVDQILRHIARFEPNYIRLGGRSKDKEIKQRTLFEVRSSIPQQKQPGSQKVQATIAMRKLINACQMLLAPLEANKPPLDHKILLSLKLITKEQAESLEMDSQCTLGISSTDSPGVLMEQWLGRCLAPCTRPILPDDYEWGYEEEDFEIEQLKELEAEAVAQDDDDKEALRGPVTLLSDNYTGKGGSLLTAIEIRNLLQETDDLTMIPVPDRGAIYNYFKREAKKLIVAQVRKIAQEYQKTVPQRKVGLWEEDVRVLRDARIIGCTTTGLSKYRPLLSALRPRVCLVEEAAETLEAPVTAACFPSLEHLILVGDHQQLRPHTQVRAFENEPYYLNVSLFERLVWNDVEFDTLTRQRRMIPEIRRLLYPIYDDTLRDHDSVKDLSNRPPVEGMGGNNSFFFCHEWPESRDANMSSVNVREAEMIVGFFDYLVLNGVDPVSITVLTFYNGQRKTILKKLREHPNLRAYPGIQVVTVDSYQGEENDIVLLSLVRSNRYHSIGFLGSDNRACVALSRAKRGFYIFGNAELMACESGTWGAVVEIMHTDKKVKVKTGQKRRLGYLFPLECSNHARKIWMEEPDDWDCIHGGCDMKCDGSLPCGHRCPYRCHPTTEQWEAYANGGVQADDAEVLQKRRQQEAQFLRSTRTVTPSTAGPSSTSARLVEISPDKVARSMHTDLLLDLGETTDASHTQGRHHRQDFSYANAASLNRKAKDKSQFNLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQC1</accession>
    <name>A0A9W9CQC1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_001950</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQC1"/>
    <dbReference type="OrthoDB" id="194139at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507:SF1">
        <property type="entry name" value="FI18259P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507">
        <property type="entry name" value="ZGC:174356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="339"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="384"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="470"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="40"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="514"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="520"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4374872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="535" mass="58836" checksum="BFDE54A7336152DC" modified="2023-11-08" version="1">MVAAQASPRGSREGAPLLSPTDLATPLPDVLAPPKKKPWVLLVLLVFALVAIVDVGAFLAEPPKTRVFEANLCLTYYREHDASVIGSDGTIPEKLCKVDEVQQKMAMIFGWQDMFDAVPGILLAVPLGTLADRVGRKWIFTASLMGLQLSSAWVLLICYFKTLPLHFTWFSSAFLFIGGGPMVAMAIGITMIADIVPPDKRTSVFLYLTASVLVAEMVAPILASRLMEKGDWTPLLLALAIQQVGICIAFFFPETLHLRDLPEPRDGEDPDVELQPKGSYFTIKAQLRHFQTAIDFIKSDWTLCLVVFTFLANRLGRQAITLLVRYASKRYDWEIKKAAYLLSFRAATNLVSMTIFVPLLNFLLLKKFHLPPHWADLWIARGSIALTTISFFVMGIAAYPALLIIGLLIYNLGTGYNAAMRSISIHVVGGQSSPDVGKLMSTIAIAESIGAMVAGPLLNKMFEWGMDLGSAWLGLPFLGAVVIFAGMTVVTMCISVKDRDVAYVEVRSDDEDLLEDEEEARTSALEREIAPRHAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQR4</accession>
    <name>A0A9W9CQR4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBF1-interacting co-repressor CIR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_002364</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQR4"/>
    <dbReference type="OrthoDB" id="17560at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019339">
        <property type="entry name" value="CIR_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039875">
        <property type="entry name" value="LENG1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22093">
        <property type="entry name" value="LEUKOCYTE RECEPTOR CLUSTER LRC MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22093:SF0">
        <property type="entry name" value="LEUKOCYTE RECEPTOR CLUSTER MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01083">
        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBF1-interacting co-repressor CIR N-terminal" evidence="3">
        <location>
            <begin position="27"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="68"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="249"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="161"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="268"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4375278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="530" mass="60531" checksum="95231037D851DD92" modified="2023-11-08" version="1">MPLHLLGKKVLQAHEQYQRAFTDNKKSWNVYNADNIARVKADEAAAAAREAADEQRMQELDTERRLALLRGRTPPPLPEEDAAKDAGRRSDKDRDARGHDRKRRKLAGEDDTAMEIRIAASMTASKGDGGEPTNILKLRNTTSDAPLTDHAGNIDLFPVDMKEAIKRERNAEAEKEKKKKERALEDQYTMRFSNAAGFKNGLSQPWYATGQKPSQEAEKGKDIVAYEGIENKNVWGNEDPRRKEREIARITSNDPFAAMQKAQVQLKKSKEDKKKWAEERDRELRELRATQEREERRSRHRKRKTRDERDDDANETVRTYFAYPKDGSTQNAILIMTDVIGMDFPNIQLIADQFAANGYFTVIPDVFNGNEIPFPPPATFSLQEYIKTKMPRPPTVDPIYATVIKHLRSELGVQKLGGVGYCFGGKYVCRWLKEGGFGEGGLTRGSLRIRVLWMRRRLGGLGGRFLLLLLRRLTEDILRELSVPYQMCLYSDTEHGFGVKGDLSNAKQKFAKEQAFLQAVQWFDYFIKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQT1</accession>
    <name>A0A9W9CQT1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ADP-ribosylation factor-like protein 8B-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ARL8BA</name>
        <name evidence="7" type="ORF">N0V83_002409</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQT1"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098852">
        <property type="term" value="C:lytic vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04159">
        <property type="entry name" value="Arl10_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001015">
        <property type="entry name" value="ADP-ribosylation factor-like protein 8B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044154">
        <property type="entry name" value="Arl8a/8b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45732">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45732:SF7">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="130"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4375323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="183" mass="20616" checksum="84AA3160A084ACCD" modified="2023-11-08" version="1">MANLFRRIYDWLLRLFWATEMDITMIGLQNAGKTSLLRVLAGGEFTVDSIPTVGFNMKRVQKGHVTLKCWDLGGQPRFRSMWERYCRGVNAIVFIVDSADRDALPVAREELNLLLEKPALEGIPLLVLGNKSDLPNKLSVDELIESLNLKAVSHREVSCYGISAKEETNLDAVLQWLIARASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQW5</accession>
    <name>A0A9W9CQW5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000156</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQW5"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053083">
        <property type="entry name" value="TF_kinase-domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305">
        <property type="entry name" value="SI:DKEY-192D15.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44305:SF24">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE C03B1.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="30"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="391" mass="44751" checksum="E66D01CA6B4EC3CE" modified="2023-11-08" version="1">MPTSEAAHAAWAREYVTRRVTLKRGDPEPFKTGQWLGKGGWSEVYETGIGGTVVALKRVYFRNTPRERILCLNELDILEKMSGRRHRHVVELIGSYELPLRGNNSQLGLLVWPVAQCDLSRFLGCLDLLGRLFERITADKDQAWTPKPEEEDALDEISRLNLFPWDKKIRKTQIQEGRYSRFVLMDLHRDCVYRLFTMVGCLAQAIAYLHDDQKIRHKDLKPSQVLLSSDGLWLTDFGWSMDVSELSNSATNNGGATSVKYHAPERATRGQRFCGRPEDIFALGCIFLEMTYRMWWMVAEDYLNPGAESGWSYQANLDLKTYWLEPLQIRARASRPPNPEIEHLTALIDQMLDPEPTTRPRIRDIVDTLAVDLGDSNPFFGNCCLPVKESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR43</accession>
    <name>A0A9W9CR43_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Methanethiol oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001324</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the selenium-binding protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR43"/>
    <dbReference type="OrthoDB" id="10033702at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008430">
        <property type="term" value="F:selenium binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011048">
        <property type="entry name" value="Haem_d1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008826">
        <property type="entry name" value="Se-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05694">
        <property type="entry name" value="SBP56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51004">
        <property type="entry name" value="C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0711">Selenium</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040935791" description="Methanethiol oxidase" evidence="3">
        <location>
            <begin position="18"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4376043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="465" mass="50670" checksum="5D391DA72F3BBE4A" modified="2023-11-08" version="1">MHSTTLLSALLASTASALSLPPLIPSIPGVTEPLSKNAPPLPILQIPTPALDSPAHEVNTQIKPKKIGYFWTSAGDNQHKDFLATYSLDDDTFGTLIAITDVPTSGNSPHHLGPSLDGKTLVGGGLLSLLKTQDTAFYFDTTNPYQPKFKKSNRALLSSITDEIRAKPDGGFFITYMGSAVGTSPGRLVETDADFNIIHEWPEDVEGTLNVLGEQFSPHGLSIDFRNNIILTSDFVVPLTILKPASINGIQRANTLRLWELSSRKIISTITIPDGQGIQDVKFIPGNKESAALATAVGPGQVWIIYPFRKNAQGQQGVAELLFDLGPRARDNLAIYSDITDDGKFAYFTLTLGNHVAALDISDLDNIKRLDNPDEDQGIVGPHYIKVSPDKKNLLVTDYFVQGGDISIVNTPADFKAQWIDILEDGSLSFNRSINFLSQFPERGGGWPHSAVIFDLSDPEHPYYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR67</accession>
    <name>A0A9W9CR67_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Micro-fibrillar-associated protein 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001370</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR67"/>
    <dbReference type="OrthoDB" id="1111734at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033194">
        <property type="entry name" value="MFAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009730">
        <property type="entry name" value="MFAP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15327">
        <property type="entry name" value="MICROFIBRIL-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06991">
        <property type="entry name" value="MFAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Micro-fibrillar-associated protein 1 C-terminal" evidence="2">
        <location>
            <begin position="122"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4376089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="412" mass="46794" checksum="D6B547F706A7EB82" modified="2023-11-08" version="1">MPPPKSKKLTAQPVRTRQFAGKPGAVEAEESSSSSEESESEDEQPKQKPFTKPKPAPVASSFPKSALKSKLASRQKSEPENKALEEEFETESEDEEGEDGSASGSGSESGSGSDESSEEESSSEEEAPRKLLRPVFLKKNERNQGAASVKSAEEIAAEEEARRQEQNRALIQDQIEQRIAEKAAGKRDWDDDVEEADINAIDDTDGLDPAAEYAAWKLRELRRIKRERLAIEEAEAERAELERRRNLSAAEREAEDRAFIHQQEDERGERGQMQYMQKYFHKGAFFTDELKELGVDRRNLMNARFEDQTNRDVLPEYMQIRDMTKLGKKGRTRYKDMKSEDTGQWGDLGQDRSRRNKDDYGVDDRFRPDSFGGRDRDREGATGANAKPLGERKRLGGDNDRDSKRPRIEERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRE8</accession>
    <name>A0A9W9CRE8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRE8"/>
    <dbReference type="OrthoDB" id="3783520at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="293" mass="34237" checksum="6459DD853339339A" modified="2023-11-08" version="1">MKRRLETVRKQDQLRWEREQAWDETHQQYDPQSAQAAAGVQSAAECPICASTVAPYPTNVPKMEVFKDVPWWERPGALAQEHILVPRAVPRMRRQPRPYAQGTKGSEALREIVGKLRVSMHAADAHRRMWEIQCKTESAVRRKHSLDESFQFQPDFWNSPISGLVSRRNFQQIQDNQRMAERRARGNTPRPKPPRSSLSHSELSEELQVDKDWTETTKKRNKQEELEKEARKVAQEIGYLYFVGAIDGMEEWKEDYLRSDRQLVIRKPVTISDSSESWDSDDDDVFDEMDLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRU3</accession>
    <name>A0A9W9CRU3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">t-SNARE affecting a late Golgi compartment protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_000263</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRU3"/>
    <dbReference type="OrthoDB" id="546861at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21442">
        <property type="entry name" value="SNARE_NTD_STX6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15851">
        <property type="entry name" value="SNARE_Syntaxin6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.90:FF:000012">
        <property type="entry name" value="SNARE domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000006">
        <property type="entry name" value="Syntaxin 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015260">
        <property type="entry name" value="Syntaxin-6/10/61_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09177">
        <property type="entry name" value="STX6_10_61_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="13">
        <location>
            <begin position="151"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4377438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="243" mass="27599" checksum="72B89A182D7BC57C" modified="2023-11-08" version="1">MMSSQGDEDPFLQVQADVLAALNTSRPLFKSYLRIRSSASSANSPELREARSELEQTLQELSQDLEDLVESVKAVEHDPYRFGLEIDEVERRRRLVKEVGDEIENMREELQQTVDNARNKGKGTANGDVLPDPDSFEEEDNYAAFEQERQVEMMHEQDEALDGVFRTVGNLRQQADDMGRELEEQGEMLTDVDNVADRVGGKLQTGIKKVGWVIQKNEDRWSSCCIGLLIFVLILLLVLLLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CS25</accession>
    <name>A0A9W9CS25_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Chitin deacetylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_000522</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CS25"/>
    <dbReference type="OrthoDB" id="407355at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10951">
        <property type="entry name" value="CE4_ClCDA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00035">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.10">
        <property type="entry name" value="Endochitinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001002">
        <property type="entry name" value="Chitin-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018371">
        <property type="entry name" value="Chitin-binding_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036861">
        <property type="entry name" value="Endochitinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46471">
        <property type="entry name" value="CHITIN DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46471:SF4">
        <property type="entry name" value="CHITIN DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00270">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57016">
        <property type="entry name" value="Plant lectins/antimicrobial peptides"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00026">
        <property type="entry name" value="CHIT_BIND_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50941">
        <property type="entry name" value="CHIT_BIND_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2 8" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="7" id="KW-0170">Cobalt</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040781024" description="Chitin deacetylase" evidence="9">
        <location>
            <begin position="20"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="10">
        <location>
            <begin position="68"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="11">
        <location>
            <begin position="146"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="83"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4377693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="378" mass="40313" checksum="6E7B6F2DDCE4D54E" modified="2023-11-08" version="1">MHYSTALAAVAAVAPMVSAHGGPVPKIVGLNPRDLKARDLLSGLGVRFEGANNFARAPAGKMKPRQDDLECGANIGTCGAGLCCSPSGYCGTGDDYCYSPGCQYQYGPGCPDNAIPAGTNTSTVTRTKLGSVAYGGDGIYGCTNPGDIAITYDDGPQITLTAYILDVLASHGAKATFFITGNNLNKGQIDETAEFIADIKRMDADGHQIASHTWTHLDLSAISQLDRKNQMWKNEMAFRNILGKFPTYMRPPYSSCTGQCETDMADLGYHVTYFNVDTDDYEQDDPTLIQNSKNWFYGNITAGGATASGSSWLEIGHDIHTQTAYNLTDYMLTTLTSLGYTAVTVGECLGDPSTNWYRTASGAGTYTSTDSDGAEPNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XX30</accession>
    <name>A0A9W8XX30_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">AKL1</name>
        <name evidence="14" type="ORF">N0V83_010878</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XX30"/>
    <dbReference type="OrthoDB" id="2018507at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14037">
        <property type="entry name" value="STKc_NAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000441">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967:SF57">
        <property type="entry name" value="AUXILIN, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="49"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="378"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="484"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="674"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="716"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="348"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="391"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="488"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="522"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="605"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="722"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="747"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="816"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="836"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="957"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="1016"/>
            <end position="1027"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4361937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1061" mass="115781" checksum="110A09716E7CDC78" modified="2023-11-08" version="1">MPSAPYAPSSALHMAQQAAYRAPAAIAVSNAPPGTFASGTKVQVGNHRVTIEKYLSEGGFAHVYLVRVPKSDGRSAETAVLKRVAVPDKDALANMRTEVETMKKLKGHSKIVTYMDSHASQLKGGGYEVFLLMEYCSGGGLIDFMNTRLQHRLTEPEILHIFSDVAEGVATMHYLKPPLLHRDLKVENVLITTVSGNKIYKLCDFGSTAPPRPAATTAAEGRLIEDDVQRHTTLQYRSPEMIDVYRKQPIDEKSDIWALGVLLYKLCYYTTPFEEVGQMAILNATFKYPSYPPFSDRLKKLIGWTLRENPQHRPNIYQVVAEVCSMRHRACPIKDIYTGRTQSEARRNQQLPPTEPQVSSPPMIGLQKVAPVKQVQQIPDITPMRRGRPTAPAQASPAAKPSPSPMRGTTSDPFAALDSQDVQVRRAAADELAARFPSLDEFSLLHDRGQKFEFGQTTPSSDPALTKRVTDALADDAFALSPATKVESAPMPKPSSSMSSANVSRSTSVKKPRPYETKDSARISTPTIQQPIPQHVNMVSTGVQTSPRSSPAPPQQKYPDVNKTPVWKVPVSSHHHRAYSQPRTFEKAQASPSLKPEFALPTRPSLDSRSKSQMSTLSIPKSPASSRPSLESQRPSALDLGYTIDRSKSANSNARPVSMHVASNLDYLRDREMAPGRNFDAPSLQRRLTSTAIDDDPDQEEKNIRSSVDFLRQIEKEDPSHKHNHRRSSSQSKHAKRGSITNIVKGKFGDAFRRFETNHSSKTPEHERDQPPLTPTDQLIDPRGTNALTPIAGSEATGGLSDDDRSAIDETQELSPEIRRELEKRRLEEEERRVEAAAAEYRQRVAQQGPGKPKAGPSKASTIQNRVKNLLDESTKPAATNRTAEGYGKYTDTSGKPLPTRPQDQSGAARQAPPAIARKPINVTTSSSSTSLNQQPQDLMYTKPSSVRPNVPPPIPSSSAPPTINPRNPSGPPPRAPKPKALQTGGSGVSIVSGSSPVKASPAVVGVGKDKPLPPHGGGGGDAAGGGDDFDVETFSKRYPSLSGLEMVETEIPRGRSVRDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXZ1</accession>
    <name>A0A9W8XXZ1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7962 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010819</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXZ1"/>
    <dbReference type="OrthoDB" id="202840at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.110">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058268">
        <property type="entry name" value="DUF7962"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25907">
        <property type="entry name" value="DUF7962"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7962" evidence="1">
        <location>
            <begin position="93"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="321" mass="35753" checksum="20976E75468558DB" modified="2023-11-08" version="1">MLPRPLLTHTFALSYRKIPVLAIGREIYCDTSLIIEALEHFFPLTQGYGSVYPKFEGVDEWTYRGLVRGFASFWMDKPFFRTTTGLIPPSVWSSSFGTDRAQLIGHTLSPAKLGAKIPQNLSSLDLHLSLLEPTFQAGTWAIPTKTPSLADISLYYQLRWGIDIASGKGIYNLSGGGTEDTSTGVAEEVFNKERFPGLWRWFHAFEAYIASLPDLQVTSDESWKQALRQTPLLPEDQVLVPAAVEQHPSLDAQRGLVPGVSVSIVPDDTGRRNPTVGRLVRIGVEEVVVTPIEKAELDVRIHFPRLGFVVKVVELYFTNPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYN0</accession>
    <name>A0A9W8XYN0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYN0"/>
    <dbReference type="OrthoDB" id="5140048at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="378" mass="44302" checksum="1E212ADC6E1495F1" modified="2023-11-08" version="1">MAKGELLTIGAAATAIGGAYKLGEFMYKAKRIRDVGPSNAVYVRIIGRVRSDLDEVRRLLSVREVHDALESNPEKSKWVYGAMRDVRGALENITPHTERVAGDVEDGRRVGIRHRVYWLLSEKEKLENREKELNIAHASLSEVLGYLTALEPVEEPQRPKSSSKKETHVDIDIHREAPQHTTERVDREVWVERDAAPRRVEEHRDVWVDDRQGPPAHYEEHRDIYVDERERGPQHHEERDIHIERSRDPRYEAQYDERYEQQDYYERRPQYPEERRHDDRPFPEPLGRRPESFEDRLPERDTWMQSDARNRSRGGQYGPYGQYAEYGAPQPVGAAHYQRRFQGDAGFGDEEILNPQPVPLDRKYAEREVSGYSRFSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYV1</accession>
    <name>A0A9W8XYV1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosomal protein L32</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RPL32</name>
        <name evidence="4" type="ORF">N0V83_010617</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYV1"/>
    <dbReference type="OrthoDB" id="268693at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_eL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036351">
        <property type="entry name" value="Ribosomal_eL32_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L32 AND DNA-DIRECTED RNA POLYMERASE II, SUBUNIT N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52042">
        <property type="entry name" value="Ribosomal protein L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4362523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="64" mass="6912" checksum="05ABBA11E01AE749" modified="2023-11-08" version="1">MPSGHKAFLVANVADVDLLLMHNTTFAAEIAHSVSARKRIEIIARAKQIGVKVTNGKARVKTES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYX8</accession>
    <name>A0A9W8XYX8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009659</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYX8"/>
    <dbReference type="OrthoDB" id="3791583at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="77" mass="8650" checksum="103EA5BBF75A3A6C" modified="2023-11-08" version="1">MVKSEKSDIVTSSSAVSVTSSTSSQNTVVASKAKGVWEAIKRHHKDMNQAFEIYYGQGQLRDGRKQEVWEYKRGGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ92</accession>
    <name>A0A9W8XZ92_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_010901</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ92"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053181">
        <property type="entry name" value="EcdB-like_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47785:SF6">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47785">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="51"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="119"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4361960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="690" mass="77872" checksum="CDF333D75D3ED6C1" modified="2023-11-08" version="1">MTKRSRDSITSSSYAEPSDRRASHDAEGSGSHEDGEGSSLKKPRSFMATLACDICRARKTRCDEDRPRCGYCRTMNLECTYQTPRATKKDQSMAVAINAIRRLESKIEDLTAAINAPQAPAAMPLPPPGPLPDHRSPQSTYSQPSPGSHGPIAHSHRSRNDRISIHELAMSPQVMGTPAKSPYSVPETHSKVALSFSQHRVALWPSVKQILPTEFVSARTELSEDYIMEIENQRAPLPMHIDRPPNTLRDSWLMDLQQSVVKGLSDAYFAVFHRNTPVLDKFHFHATTLGLAVENEFGCDIESCLVLTVLALGCLAVKAHEEGNFALPSRRSSTVHGFIYPDWYELTLDAPPGLKFFNEARKRFGFLMCQNDLQAGQFYMLQTLYYAQILRPLDSWTTVNRAALCCTSILSRTNTTDFDKWEGDMLSRLFWNTLMYESVITQELDLLPHSGLLNYEIETPLPKFMPCPRPKTSISGLITDEEDSLFNFHFLAQAAHRILLTRVKQSLYFIAERENFPSASLTAEMHHQLEQWRASLPQTLQFSDNESVDESPSPAHVIAKAMLRSRYLVAKFHIGRPFLYKALHAPTYTNDAEYNEVREGLRGGMYWPTTMGLCTQMLSALPLKFGWCCQCFGQVLLFHAIARSPDANLQETLPAGWQEWVQIMMKLIESCARDSPGIAKDFELLRLLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZA6</accession>
    <name>A0A9W8XZA6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_010148</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat SEC13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZA6"/>
    <dbReference type="OrthoDB" id="5566198at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035859">
        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037363">
        <property type="entry name" value="Sec13/Seh1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN SEC13 / SEH1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024:SF3">
        <property type="entry name" value="NUCLEOPORIN SEH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="4 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="137"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="460"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="38"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="390"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="38"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="390"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4363030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="511" mass="56850" checksum="5C0A38D210BFE08E" modified="2023-11-08" version="1">MDPNPPAQHSQLDAIQLNLDAVTRRLVRPTFLSNLTPSRLPSSAVSDSSSLSPTRDEARLHSGPLSHHRHSHLNFAVTFEDVLEHADLVSPLIQEPCADGSPSSVDRRVVVHLESHFLAARLAYKKMAAERNFQTFSHGHQDLVLAVDFNYFGTRMVTASSDHRLKVWDKKDDSWTLVESWKAHDAEIVDVKWNGPFMGEVVGSIGEDGRCKLWQEDVTEVPMSGNRFKLITNLASQTHAPFMSLDFKNIMQETWLALITRDGYLTVYEPQDQSSLNEWTILAERWVSGDNPPERQDEVGFKVVFHKEKLPCWTAIMAGLDRKSLSLAVAAMKDVLVFRTDKAKRFFQVAKLEGARQIIRDLAWANGSMRGHDILATASKDGAIRIYELSTPTSDKTSPGQTSMASADTDAISPRHGPRKNAPSGIGAGLAGASKATDTTIENEQYPGRIRQVAKLTDELTNHHGAVWRVTFSQMGDLLVSTGDDASIRTWKKAVNGKWTEYAEIETARDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZD9</accession>
    <name>A0A9W8XZD9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_010778</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZD9"/>
    <dbReference type="OrthoDB" id="406156at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Non-haem dioxygenase N-terminal" evidence="3">
        <location>
            <begin position="69"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Isopenicillin N synthase-like Fe(2+) 2OG dioxygenase" evidence="2">
        <location>
            <begin position="248"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4361837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="414" mass="47084" checksum="2B73F0B207E0E2EE" modified="2023-11-08" version="1">MELLRCRLSYFTAPGSHSHLITHSSKTLVSDLQIYEMTSTAMNAVPIPPKYQDVRPTSADLDYADLITLDLSLFDTPTGKQRLASQLKHAISTIGFFYVTNFGLTPQQVDSQFNIARSLFSMPDTEKENYRVDPKKQGFFGWKPRGSRKAQFGLKDNLELYDDPKYNATHGDIERPAALAENRRECEEFGRHMHFHVLYRLLYICGMLMELDDPEELWHKHNYDEASSCHLRYMLYHPRTQAELEIMKNNELEENVYGHTDFGSLTLLMRQPVAGLQARTVNSKGEIKWKWVRPLDNSITVNVADTLSFLTGGYLKSTVHRVVLPPEDQRHIPRYGVIYFAGPDDGTKLRPVASPVLEGEKKEAEAGLQKRDLPPDTTTAGEWFSVRFGSIGKNYETNKDNAVSVGAKEVQAYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZP7</accession>
    <name>A0A9W8XZP7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009769</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZP7"/>
    <dbReference type="OrthoDB" id="106784at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="604"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="203"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="621"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="659"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="680"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="731"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="771"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="825"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="899"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="963"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="983"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1001"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1021"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1080"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1135"/>
            <end position="1155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1155" mass="128459" checksum="2FBB751EDF71E6D7" modified="2023-11-08" version="1">MANPAADPKAAPLADDLKGFALSLPTTVFPKNFFCARCTQLAVDSWKLLCCNKVICSSCQVALQFPTACPLCDHSPLEAESCTPNKALRNTMRVWLDRRKKKEETKAAAQAATPLGDGAPAAPEVQPTDDAADRPVESVDQVSMAEVVPMEQTSAAADTVGHVTERLASASVQPNEDNTSPRVDSERRGSSALQHVDRSTEPTAADGATDGNAANASIGGVMANQPMMNGMQDQMGFGFPNQAGFNNGMGWNSMNQMNGMPNMMTNGNWNDFNTMNGMSSGMYGNFGGNMGLGMNDMSAMNMMNFGGGYGNGWNGMGGGYGNFNGHNQMGGYNQSGAYPEMMNQFPKNSFPNQNQTRFHANQGGAYPQRNNRNGSYGGGPGFQNANSRPGSRSGPAQNVRRFHHHLPPRPSPMAGKSSDSTTDEYLLKQRDGQSPDGTATATPDVKTEEEQRKVSAESTEEGNDIMDGADPSVDPSAAQKASTGETSENANETTNQAEDDSDETAQGGGELSRIQTVDSGDADMESYNQSTMGSGMHASMPYPQGMMDQFASHSMNASFDSSLSMNMGYDHNHNIGPRGNFNNGAYGAARVLTGQPTEPIGVGVVGAPTGPRAMREGRPNTGFSSRMNSGRYNPPAKSITPAQDPVPGSPQRRVRSRSPQRDENLRVKERSSTRSRSRSLSREREGDRDERRERSRSADRESRREGRGRSPTPQSDDDYERRKERRHHRSSRYDDRDEDYDGRHRDEKGSRDDRTRSASADSKYRSSRRDKDKHRSSRSHRDRSKEHRRRHRSRSPAEDKYEDEEAYTNGEKESDGSSRRKHRSGDKDKYRERDRERSRDRDRERDRKDRKERDREYEYERSREKDKDRKRRRDRELEDDARDYEDNGYRSSRRSRKDRGRERDRDCDYDDRERKEKPRQQENEEDVVGQTLKKRPISPPLNAPTGPSANGFSIKGRSKNAIMPPPVQPPTGPRAFQPPKGPAADRNKERSDSRDHRRKSSASSVPSGPSTPATSATQDHYAAERERNARERDSRDKVDKSNRDVLTKSLHTRVHSSSRPSLSSKRSRDDIEDAEGSEATARDIPRGPRADVKAPTGPASHRDKRRKSGATGDDNIANLFTAGLRKNAKARRGGVRTEGDVEREMERVERERERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZW3</accession>
    <name>A0A9W8XZW3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Non-homologous end-joining factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_009573</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the XRCC4-XLF family. XLF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZW3"/>
    <dbReference type="OrthoDB" id="2155935at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032807">
        <property type="term" value="C:DNA ligase IV complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045027">
        <property type="term" value="F:DNA end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22285">
        <property type="entry name" value="HD_XLF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.210.10">
        <property type="entry name" value="DNA double-strand break repair and VJ recombination XRCC4, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052287">
        <property type="entry name" value="NHEJ_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053829">
        <property type="entry name" value="XLF-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015381">
        <property type="entry name" value="XLF-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038051">
        <property type="entry name" value="XRCC4-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32235">
        <property type="entry name" value="NON-HOMOLOGOUS END-JOINING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32235:SF1">
        <property type="entry name" value="NON-HOMOLOGOUS END-JOINING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09302">
        <property type="entry name" value="XLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21928">
        <property type="entry name" value="XLF_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XLF-like N-terminal" evidence="9">
        <location>
            <begin position="4"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="XLF-like coiled-coil region" evidence="10">
        <location>
            <begin position="128"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="259"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="282"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="293"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="336"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="388"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="408"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="428"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="473"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="490"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4364118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="536" mass="59381" checksum="B9E3AFE25D39110A" modified="2023-11-08" version="1">MSCWRVLQLAEQPDSEQIPQLLVKPAFSPDSYTVHLTDLSHIWSEELDLEGVVRRASQEQSPIEVSKQDTAQLAILLDNVKKSLDSSDDANCRITRNEEEGVVLHTSIALPEPLESLTWRFCFQKRTSTTLKNELILPLLVSSHVQHERITGLISTITSKDKAITRLVDQYESCNLDLAAAFPSVGSLKAGRKGIKREQAAKHIPALQPFREAVWRKETGQLVESDVTTLGLFQEALAQSTPKVPPQLKSEEHETSWWAAAPKNLGPSKPDSILKAKKPKKPVIQPKKIVTESSEDETEDEFETHENFKTRNLPSKSTDAREPASSPPHTNQATSDGDDTEEDDDLDAPAKSQSQKQTRTPRKPQPRAVSPTPEVSPPTKVSTPPIAKSKKAFRIGGKAKKATADSPPLSQTDQNVTPDAEDTAEKTIPSSPQAGSQTDATPKKVRRPFKIGGKGKDDSQRAVLASPKTNRVTTTRSPTSELPSSPPPDRVVKEETPDEVHEETPEERAERKRAELKRKNEEAAKKQAQQRKKKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y020</accession>
    <name>A0A9W8Y020_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MFS general substrate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_010440</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y020"/>
    <dbReference type="OrthoDB" id="196103at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010291">
        <property type="entry name" value="Ion_channel_UNC-93"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051617">
        <property type="entry name" value="UNC-93-like_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294">
        <property type="entry name" value="ET TRANSLATION PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294:SF59">
        <property type="entry name" value="UNC93-LIKE PROTEIN C922.05C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05978">
        <property type="entry name" value="UNC-93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="338"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="467"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="467"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4362347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="486" mass="53099" checksum="66576614DFCAD62B" modified="2023-11-08" version="1">MGFLNLPQLRYHSPWVQNFISGIGVGASAGIYVALNLLGAGGGRPDSAQVVQVVNATLCAVWFFSSSFGGSILNKLGPGITMCIGVQTYAVYVGSLWYYDETGKKGYPYAAGPIIGIGAGMVFITAGYVATGYPEEKEKGSYATVLMNMQALGSVISGIIPVIINRNSETVAGVPRSVYISFITIMIIGGLITLLLLRPQKLTRDDGSVVAVHPPRGAWEELRSNLLVFTDPILLMMVPAFLPAEGFLVYSGSVNAYNNNLRTRSLLSFIAVVLQIPAGWALQAVLDYPGWGRRKRGLIGLTGVAVPLVIAWVWEMIRTRNYDRNSPPTNPTDWDEPKFGWIFVLFMLTWVSSQLWHNVVYYWIGAITNSPQKLTHYVGVFRGVLGAGEAICFGLDSIKIPFTAEAGGILLFYVIGIAVFYYLGIYHIKDTNYDHTEEGVVVPNHVLEGEGLTVGQRREEILRNRQVDGSGEDKLEQSVQVNSDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0G1</accession>
    <name>A0A9W8Y0G1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_009375</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0G1"/>
    <dbReference type="OrthoDB" id="4161376at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06179">
        <property type="entry name" value="MFS_TRI12_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010573">
        <property type="entry name" value="MFS_Str1/Tri12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053791">
        <property type="entry name" value="MFS_Tri12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF109">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06609">
        <property type="entry name" value="TRI12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="363"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="393"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="423"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="543"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="59"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4363922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="595" mass="63483" checksum="68D72FC6B983E9D6" modified="2023-11-08" version="1">MASEKKYTEEVTPATASPLTHQHTASSHGLHHDHIAEEAYGGHTADLGKSYFRSVNFIGTVVATCLAQISGYLGWVLPANTLSLINAAIGPSPDIIWVGISWTAGFAIGFTLVGRLSDIFGRRWFFITASLLGLVGNIIGASAQSINMLIATNSINGLAAAGQLSFHIILGELVPNKLRGPVNAFVLSTSVPFAVFGPPVARSLYQTTALQWRWCYILGCIVNVLAIVLYFFFYHPPTYEMLHVGGKSKLKQLRTLDWLGIFLFSVGLVVFLIGLNWGGSAYPWKSGHVLGALFAGFGTLVIFCFWEAYAGLEYPLIPMRLFKNIKYDANVACASLAAIVYYANSIIWPTMVSSLFTTDITEIGWLSCAVGGGLLLGQIFGGAGVRFLPKMKIQMTVAAVFTTAFVGAVAASTPNSRNQTTGLLLIGTIAAGYIENLTLSSTAYLWDPADIGLVTGVMGAIRTGLSAIATSMYSSILSTEAAKYIPQKVMPAALAAGLPSSSLPALFSGITAADFSSVPGISAEIIAATSDAVKEAYALSFRTVYLCTIPFGVLLIIAAVLSPNVEEFLTDEVARKMHGHTDKTEVKQKEIVEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0H3</accession>
    <name>A0A9W8Y0H3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0H3"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="279" mass="31565" checksum="395268EDB8264CFE" modified="2023-11-08" version="1">MPSSKGKGVAGKNAQQARVESPSAESEWTCVSGYDDAHDYEDQNQNLSWEWSTSKVTYEPRIATQQPEQPAEVQAQPVEQHHDNVYRGPLPPEPAPGQDLYIKIPPQTSKIIFQRPQHPGIMEPLEEDYRRLGFHDDHHERQTHLDSAMPTGFAPLHAPSYGGIQGCQRQPRLAIPTTRHLYHDRSNLLLPRRFHDGTNELPGRGKNRGRRAANAFAQIPAAIGPIHSLELTSVMENYMEAVEKVYNELSAVNCALFRLSAMVKRLEQKDAENECIYMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y181</accession>
    <name>A0A9W8Y181_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="6">2.3.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LAT1</name>
        <name evidence="10" type="ORF">N0V83_009102</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + acetyl-CoA = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:17017"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="2.3.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="6">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.12" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y181"/>
    <dbReference type="OrthoDB" id="537444at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004742">
        <property type="term" value="F:dihydrolipoyllysine-residue acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000010">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000003">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="entry name" value="E3-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045257">
        <property type="entry name" value="E2/Pdx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006257">
        <property type="entry name" value="LAT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01349">
        <property type="entry name" value="PDHac_trf_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151">
        <property type="entry name" value="DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151:SF90">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3 6" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="8">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="9">
        <location>
            <begin position="148"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="85"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="90"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4364507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="437" mass="45765" checksum="D24B2D211196DBDC" modified="2023-11-08" version="1">MPALSPTMTAGNIGAWQKKVGDSIAPGDVLVEIETDKAQMDFEFQEEGTIAAILRDAGEKDVAVGSPIAVLVEEGEDVSAFEAFSIEDAGGDKKPDTPSKEGDASEASEPADTGSKTAPPAKEESAPAAIESESTGDRLETALQRQPAVSPAAKKLALEKGVPLGAIKGTGKGGQVTKEDIEKYKPAGGASTAAGVPAATYEDVEASSMRKVIANRLTQSMQQNPHYFVASNISVSKLLKLREALNASADGQYKLSVNDLLVKALAVAARKVPAANSSWREDSGKVVIRQHNVVDVSVAVSTPVGLMTPIVKNVNGLGLQSISSQIKDLGKRARDGKLKPEEYQGGTITISNMGMNPAVERFTAVINPPQACIVAIGTTNKVAVPGELTEDGTPSIEWDDQIVITGSFDHKVVDGAVGGEFMKELKKAIENPLELLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1G9</accession>
    <name>A0A9W8Y1G9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009474</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1G9"/>
    <dbReference type="OrthoDB" id="4115400at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="436"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="694"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="470"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="505"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="560"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="630"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="694"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="749"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="804"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="842"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="896"/>
            <end position="909"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="941" mass="101756" checksum="15D9060092D5B0EE" modified="2023-11-08" version="1">MVFGISHEFFDGLKKHHGKLPKERKGVISSLPLPSSTRTADTNPEELALWSNALDSLSRDGLPKNHEIESESGDDSDSQPSRPATPSSIETESEDDSVEESEPAAPATPQAAPRGGRGRGGRGGRGGRGRGGRGRGRGGRPRGGAARAISPVRARPSRNAAPMVPLTEEDDDQPSNQTTPNENAKPPLRLLSIEPAPDSNDAEVDTEQRIDDEESEDMRGPLGSRQHQSTTPDGSPPPNLLESILDGTYTTKPTMAATSGLQPDRKMAAGNKLSIPRKALKTKSTSQTPREGTSTPAAPQSLDPADDRLTESDLPEPWIENAPLPIEAECEDRADYLLQKRFKPMTDVQAIIAAINKCPPSQRSTESLYALAKNSQEILKAWQDEYLMLDARTAPHMHPAKKACNGGRIPLATEIYEDMKEADLYGYVYDPKKLPGHQDPWAQRPGAEKSGGRELRTRRTRDMLDSAAPSEDDEEEDEEGRPAKRQRRATRKFDGTDSGNGANTPKKHNGWGGARKKGVSRFAKPASETPEPEGRLTKRARTAASNLLHQRIQEMREESLLGSSGDEGSSAMDVDEYSDTNPKRGRPAGSKNVARRSDYGVKKGPRKKNSEVGTPTPSAHGPNAPPLALNSMSEGQGQFTIDAQPHPDMPLQVPNSAETVFQATPQVPASQETPLIHPSESTTPDAYMITAPLSQYTNNYVEESTPTSGSRRKPRVKSEKRSHSMTIWWAERKARKREQDEKNGTPAKAPTSRSNSSSGRRGGRLSAGSATATTPQATSSEAQQQQSSPTYHPQHPPPEAYAQYSGQAPYSTPSQHYMYVSAGPPHPMMMQYSPLAALPSGSFPSYSTAPPSTSQYGSRTLAPAPMPLQHVPTYPSPYGPQSPAGSRPKSSGQPPRSGPPALAPAPPQHFSPYAPVSADRQMPFKVMIPGPAPTEARRESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1J9</accession>
    <name>A0A9W8Y1J9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_009534</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1J9"/>
    <dbReference type="OrthoDB" id="2154091at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051615">
        <property type="entry name" value="Transcr_Regulatory_Elem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31313">
        <property type="entry name" value="TY1 ENHANCER ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31313:SF81">
        <property type="entry name" value="TY1 ENHANCER ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="49"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="115"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="697"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="736"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="768"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="792"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="812"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4364079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="908" mass="100740" checksum="D06C0BCA79D3969F" modified="2023-11-08" version="1">MTTESTNMNDQTDTIDVKSASDSKQEQNTSEKTSAKDKSSKPRHRASVACASCRDRRIRCVVPPGDKECTQCKRSGVDCVIKNDDERRRPISRAYMCSLTERVALLETMLKERGEELPPANHPPKTRHGTQQNDGPSPNRKATDSQSNQNDNSSPGSQPSPQDDYPEFDQHHEYNGSPHNDADSTGSPSMLPPPKKDGMVNRLLSTRGHLSFDQLSGRLRYFGPTTNCHVHSENLNPFDATQEMSEQARRADKIIRSLPLETYDYLMELFWQCYNPVIHVLHQDAFNEDREMGRSQFYSGFLHVCVLAMGFRFADKERLDIRRISLPGKESTLHREAKYMLDHELERPGGIPSVVALLLLGDLECGVGRDNLGWLYGGMAVRLAFDIGLHLDARLSGLPEREIEIRQMTLWACVIYDKYWALFLGRPTSMKSSDLEIYSLTTQFARLGTCLPSGPSKSTETLIYEALLDLMELAGKITENMDPHRSQNPESHTTVDRNQYLRMAALDREFSSWYARLPEQLRWTPNNIATAPFSFFLLHQQYHASLILLHRPFAMYEDQSARDGLEGHGPDDHFSALSRTVCTKHAIRVARIYWQHRQRFDTKQIFVTGIQHAGTAATALVAALAFIKDPNDRNNNMQYLECLSAALSDMAATYQPAERMSHVLRAVMVELRGGPEPSMNHFRLYKPSSAVVPARRGSTIDMDESESTSQVFKKRQTSRPRAGTGASGRSIGKVRTSSMSTTMSIGSTTGGSSGLTIKPPSPPRFDDTNGNNMDRNNNSNSDGGFILVTPRSEISSTTWPPSLSSQHEPPELSLSSATSSTTTASSNNPNRHSGGTWMGAEFDPQDSINQLANAHFPELNAFGDDGAGGGGGMDDGGMTNLDFMSLGEGVGGEWGLGKEWGVVLVVGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1P1</accession>
    <name>A0A9W8Y1P1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tyrosine specific protein phosphatases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009609</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1P1"/>
    <dbReference type="OrthoDB" id="428974at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004484">
        <property type="term" value="F:mRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14502">
        <property type="entry name" value="RNA_5'-triphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000090">
        <property type="entry name" value="Dual specificity phosphatase catalytic domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051029">
        <property type="entry name" value="mRNA_Capping_Enz/RNA_Phosphat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367:SF25">
        <property type="entry name" value="DUAL SPECIFICITY PHOSPHATASE CATALYTIC DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G03540)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="3">
        <location>
            <begin position="453"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="536" mass="58794" checksum="FAE4A02143AF67A1" modified="2023-11-08" version="1">MSRCGISSFEPKDWNAYTTEALVQLLAVVIEAHRAVDENQTIILVGHSMGCSLVALLASPSSPYAHVLSQHVSGLIAICPQADPPTEKQVKALRSLTSVPSTIFDLFRKWDRRGGINSKSVLRMAGTDTDEETKKLQLRFNKQSRSPVWQRMARGMCPDYSSGSPRGGLPGREVWTGLNLPIFLTAGEDDHVTPATNVKRILEFLGRDVTSIEPPSEKASLPIAAAPFDPVAVLPPGSARKHQDSGIDAEDLPKLEDDVKTMNSTATSTNDGSFVNTEGSMTTADAPLISGAPVSGSSIPSPFPRRLVVKTAIMPKPAAHSLVFAPSTSRIIAGLIGTFFADHIDPRLSLAWQLQYLSTEGKWDVKNLEKWKAVPPVSKPIAGVFRAMKTLREVDEQHTPKVLAQKWAGKLSAVMDISHDNPVYDPKGLEDGGIPYYKFPTVSKQPPQADEVKVFIDLVDKIRAEKRDGLIGVHCHYGFNRTGFFLVSYLIERLGYRVEDAIDTFQQARPPGIRHSHFIDTLHVRYCHGLQKAPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2N8</accession>
    <name>A0A9W8Y2N8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminoglycoside phosphotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007926</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2N8"/>
    <dbReference type="OrthoDB" id="10003767at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051678">
        <property type="entry name" value="AGP_Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310:SF51">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="2">
        <location>
            <begin position="319"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="736" mass="81647" checksum="E5C2BFBB6CF4ADA1" modified="2023-11-08" version="1">MLIRKARSPSSSTPQPPGNSTGQQHTVVSPPASRASPMKSASKVITTSKNLYSKCASKLSSKASQVFGRKSQASSKPPSVSSTTSRASSTSTAATTHTLPILDCGDPFKVPGFETSKFESHPNHESPSIGQMLDTPTSEVTELNSVPTGLKASDFSLEAEEVVQDKQEQLLETGTACQLLKHLKEIPTMIQVFSEGAVPEHIAGDNDHTAHFETAKELNNEESVQAEIALIESNTNNNGDGDDKDEDDNNDDGNGDDDEGEEEKEEDEIDEEDDPPRCDFKTIGAIPDAAYKNLAQICVPSTDEDQYVRISGRTRGSYNAVTFIEIEAGEEVHQLVVRVPAHGTPSQWKGQDDYTMECEVRLVEYIRKHTTAPVPEIISYSTNHDNVLGFPYILMAKLPGKPATSIWFDQDYNPADMRFAYRNADVPSVETEKKRITFLRSLAQVMTSIQQLTFDRIGTPLFTEEGEHYGVGPSYHWDENENKFIERKACSTTQDYLQAALNAFFNPQAMLGHGEKSTSHISGTRSFLRTIFAQSVFNHADPETEETFTIKHNDLDLQNILVDDQGNVTGIIDWDHARAAPRCIGAAAVPLFLRNDWFPRYANNLLNAPHMAWNYHYYRQVYAAAMLEAAGDVDSDAKYTVKSAMYQAAVAAITNDGNERDLIEKLLHVIPKCRIDVDEFKIALGRGWPDAMAMLQKELAIVFEPELPPARFLEQVNAGLELGDWWKAFDDLLLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2U7</accession>
    <name>A0A9W8Y2U7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2U7"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4366408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="117" mass="12996" checksum="2F707F75A7F0B17B" modified="2023-11-08" version="1">MDEPQSRSAAAGLSLGATGTLAIKGGTADWPRCKTNPLNLFLMFMTSFHGQDGGERHLRMTKSLFKLVDSAFHGLKGKRTYMKGHVKTGGPIEEAWYVDDRWRLVRIQRMGEKGVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2Z8</accession>
    <name>A0A9W8Y2Z8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008801</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2Z8"/>
    <dbReference type="OrthoDB" id="3365267at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="182" mass="20298" checksum="D8D1E5D95656A3C7" modified="2023-11-08" version="1">MSDSLAAFRNHRSKDLRKLAEEHIQHDLDQKDRDLLNKAGRKVSTHAGLASGIGLGLGIYLAYRLRSMRLAYFNAFRAMEKPVEVRFADGRTEPIPDLSDKLSPSRWGDAATYFLFSVGGLFLGGELGLITGSASAARTISKDPQTKERIEKAFKNYRIDVLKKELSAMEGKSKLSDMFRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y356</accession>
    <name>A0A9W8Y356_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Peroxisomal copper amine oxidase</fullName>
            <ecNumber evidence="11">1.4.3.21</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">AMO1_1</name>
        <name evidence="11" type="ORF">N0V83_007392</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.21" evidence="11"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y356"/>
    <dbReference type="OrthoDB" id="428293at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006862">
        <property type="term" value="P:nucleotide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044712">
        <property type="entry name" value="SLC25A32-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45683">
        <property type="entry name" value="MITOCHONDRIAL NICOTINAMIDE ADENINE DINUCLEOTIDE TRANSPORTER 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="59"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4366864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="157" mass="17862" checksum="D75295CAB0E06CE9" modified="2023-11-08" version="1">MSFGLRHVYETRGLKGLWAGFIPSSLGVLHGAVQFSIYENMKKRRGTQVGGQEKLSNWEYVYMSGGSKLLAGAITYPYQPIRARMQQYNAAQQYSGLWDVLSKTYKNEGFLAFYKGVIPNTLRVIPTTIVTFVVYENTKLYLPKAFSNDEEQSHNED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3E9</accession>
    <name>A0A9W8Y3E9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_008666</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3E9"/>
    <dbReference type="OrthoDB" id="47007at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48107">
        <property type="entry name" value="NADPH-DEPENDENT ALDEHYDE REDUCTASE-LIKE PROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48107:SF26">
        <property type="entry name" value="OXIDOREDUCTASE, SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY (AFU_ORTHOLOGUE AFUA_4G05870)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4365050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="297" mass="32232" checksum="BC1A0F4F885FA896" modified="2023-11-08" version="1">MSDKGQFEQGYQPEVQHQQVPGLQSKLDPKPEIDHLPTADGGRELYKAAGKLKGKKALITGGDSGIGRSIAVLYAMEGADSFIAYLPQEEEDAKETVKLVEAKGQKCYTYATDLTDRANCKKVVEEAVKQLGGIDILVNNHAYQMMVEDIKDLDEDQWVHTFNTNIHPFFYLSKYALPHMKKGSTIINNASINAYIGRPDLLDYTSTKGAIVAFTRGLSNQYVGKGIRVNAVAPGPVWTPLIPATMSDEAIKQFTAPIGRPSQPSEIATVFVFLASNDSSSISGQTIHPNGGTVVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y477</accession>
    <name>A0A9W8Y477_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">NADH kinase pos5</fullName>
            <ecNumber evidence="9">2.7.1.86</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">POS5</name>
        <name evidence="9" type="ORF">N0V83_007396</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.86" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y477"/>
    <dbReference type="OrthoDB" id="24581at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042736">
        <property type="term" value="F:NADH kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000033">
        <property type="entry name" value="NADH kinase, variant 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.30:FF:000009">
        <property type="entry name" value="Poly(P)/ATP NAD kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF26">
        <property type="entry name" value="NADH KINASE POS5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4366868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="439" mass="48179" checksum="F931173BB2BEFD73" modified="2023-11-08" version="1">MKLAHHRVLPWRLRAHAPQARHFSSTRLRLQIKRIAELPARTKPSYIQSPTNTLLSLQWPSPPRNVLVTKKKRTPNITQSVLEFTTHIHSTYPSINVILEPESAEELHGQLPFPVYTFDKHVGVPHHFPDKTDLVCTLGGDGTLLRASSLFSQADSVPPVLSFAMGTIGFLGEFKFKEYRRAFREVYMSGAPDTYATLSDSLGGNPPRPPTSPEDPLDRPLSYADIRGKAMGSNRTARILLRNRLKVGIFGPDGTRIGGAEGDGDTYALNEVTLHRGSSPHLKIIDVYINNRFLTEAVADGMIISSPTGSTAYSLSSGGSIVHPLVPSLLLTPICPRSLSFRPLVLPAETPITLRLGKKNRGREVEVSIDGKTVTEKLGTGMEVRIAGEEVRRDTGGWEGGVPSIVRGTMGKEDMAEDHWVGGLNALLKFNYPFGDQEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4M5</accession>
    <name>A0A9W8Y4M5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glyoxalase/fosfomycin resistance/dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008186</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4M5"/>
    <dbReference type="OrthoDB" id="16820at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10374">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE GLYOXALASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10374:SF19">
        <property type="entry name" value="LYASE (GLO1), PUTATIVE (AFU_ORTHOLOGUE AFUA_2G13550)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyoxalase/fosfomycin resistance/dioxygenase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="91" mass="10037" checksum="A9276CA7C2EC7987" modified="2023-11-08" version="1">MLRIKNPEKSLNFYCDVMGMHVVFIFNAGPWTIYYLGPRDVGFTVPDVSTTLSRIRAAGYEVIKPLGEAEEGTMGLPDDVVQGGMGALRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4N8</accession>
    <name>A0A9W8Y4N8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_007282</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4N8"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004032">
        <property type="term" value="F:aldose reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006066">
        <property type="term" value="P:alcohol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019568">
        <property type="term" value="P:arabinose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042843">
        <property type="term" value="P:D-xylose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042180">
        <property type="term" value="P:ketone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000018">
        <property type="entry name" value="Glycerol dehydrogenase Gcy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="8">
        <location>
            <begin position="22"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="7">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4367697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="298" mass="33331" checksum="66F4F8D5CD1555B4" modified="2023-11-08" version="1">MAPKTWTKESFTLNTGAKIPAIGLGTWQSKPGEVRDAVKAALQAGYRHIDTALAYGNEKEVGDGIKDSGVPREEIWITTKLDNPWHKRVEEGINSSLKSLGVDYVDLYLMHWPSSTDPDDLKKHLPDWDFLDTWAELQKLPASGRVKNIGVSNFAIKNLEKLFADSRFKIAPAVNQVELHPNNPSPKLLAYCKEKGIHCTAYSCLGSTDSPLYKNEKLKKLAESKGKSVQQVLLMWGLQRGSSVIPKSVTASRIQGNFELDGWELTDEEVKEIDSLPERFKVCGDAWLPIKVFFCDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4R9</accession>
    <name>A0A9W8Y4R9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_006384</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4R9"/>
    <dbReference type="OrthoDB" id="3648309at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015123">
        <property type="term" value="F:acetate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051633">
        <property type="entry name" value="AceTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123:SF4">
        <property type="entry name" value="PROTEIN ALCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01184">
        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4368029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="279" mass="29918" checksum="247C3E1710828787" modified="2023-11-08" version="1">MATHHDHQSSQDTHIDSEEKFRYELAKTVTLTPELYERLFITPKTPVAGDLRSRFGNPTPIGVLGFSVAVIPLAISFMGWQGAGGLSVATTTINIWFGGVLLIIAAIGEFLLGNTFPMIVFFAYGAHFLSYATTFIPFYNAVGFFNPDGSGFGGPGTANQTQVFLASYAFYPIVMSVLSFIFLLGSLRTNGVFVLIFILATIGFGLGGGAFLHLSRGDTVVGSRLATGLGACFFAAGVLGFYFLVALVIAIMELPIPDLPVFDLSTVIKARSRSAIKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5Q5</accession>
    <name>A0A9W8Y5Q5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_006661</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5Q5"/>
    <dbReference type="OrthoDB" id="10255969at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050334">
        <property type="entry name" value="Molybdenum_import_ModC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43514">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43514:SF4">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="63"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="373"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="6">
        <location>
            <begin position="713"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="332"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="343"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4368305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1160" mass="128667" checksum="A07151110D36B859" modified="2023-11-08" version="1">MRRTSARLFAASRCAQFHSTRSLYSEPIVKIANGTFYRQHPASKPAPGQDAPPNPPLFPNLTLSLPSFSAPNQHWAILSPSSNIRTAFLQILRGQLLCFPPTARTFPYLLSPEITEKKPKLRFSERAIEYVGFDVERKAFGGAYLSARYESLKEESDFTVERYLTGITTLNPGEEELKAKAIDEGLMRKVMKDLELERFLDKPVSMLSNGQSRRARIGKALLSGPEALCLDAPFIGLDPIVTKHISKVLHRLAEANAPRIVLSLRPQDAIPEWITHIVYAGDDGKVESLGPKEDVFQYLKQRYEEVERTGSKGLELDPKLMELREVGRHLSGRGDFEAGKAGTPQSSSNTSILSRDGYEKVDRSEVPVTEPVVEMQGVRIAYGTNSVLGDWQQEVDGSEKHGLYWNVHRGQRWGIFGANGSGKTTVLSLVLSDHPQTYSAPVKIFQRSRLPELGVPGITIFEIQARMGHASPEVHALFPKRLTLRTTLETAWSDTPITKPRLDENARQRVEACLRWFEGELNPLLKDGQTSSQNLDWATQTVFGEVSFSAQRVLLFLRSVIRNPDIVILDEAFSGMDDLVRDKCLLFLTRGETMELQYTDAGLKPVESKAAKSGKTVVPGLQEHQALLCVSHSRDEVPGCIREWVRLPEPGTGPPRFGKFDGPVELDGRRWDEIWNDGIETTLKKLKLSSEAQVFFRNQEGYVERTKRWQSWKSPDVAAVVEVATPEDVAETIRFANTHNITFFAFTGGHGAISSLSALKNSIQIHMRKLNTISLSKDGTYATIGGGAKGKEVRDALWEAEKWTTHGVCECVGFTTVALGGGLGLLEGRYGLMSDQIISLDVVLANGSSVTVSESSHAELFWAMQGAGHNFGVVTSLDYRIYDIPDAEVGGKVWSYEVLVWEATPENVKSVYGIAKEMLEGGKQPDELFLYGLLGMDPAGSGKVVIMHNAEGTFLDVPSWAEIAESSLPCRIADMLPGTGVLRFPTDIRTYNLSALAAVTQKFVALVTDEPQDFRNGFLMIEQYAQRAVRAADPGRKSSVVPWRDHGLLIAPALLYEAVNLSATPPQLNERLGKLAWRKGEELRQVVVDGAEGMGGHFAYVNYAYGGESVEEMYGEENVGKLRELKRVYDPEDRFGFYAPVGVGKENEGMGERERERDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y600</accession>
    <name>A0A9W8Y600_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_006951</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. DHA1 family. Polyamines/proton antiporter (TC 2.A.1.2.16) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y600"/>
    <dbReference type="OrthoDB" id="446368at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000266">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF186">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="171"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="203"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="303"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="350"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="417"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="450"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="485"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="80"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="557"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="564"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4367370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="635" mass="69480" checksum="4843AA26CAE79210" modified="2023-11-08" version="1">MEKEAEAGVPSNGPSSMDLESGKEGISHWKMIMDQGVVTKDIINWDYEGEGTEEDPYVVEWIENDPRNPMTWSKTRKWIMALTVANSVLVVSFCSSAFSGGIQQIMVEFSVSQEVVTLGVSLFVLGFALGPLLWAPFSELYGRQVVFFGTYLAFTAFNAGAAGAPNIYGLLILRFFAAAFGSSPLTNAGGVIADLFSANERGLAMSIFSAAPFMGPVLGPIIGGFLGMTEGWRWVNGLMAIWAGVVLICATMLVPETYPPVLLRRRAERLSKLSGKVYRSRSDIDQGKVSLGEAFSTGLKRPWILLFCEPIVLLLSLYHAIIYGILYMLFGAFPIVYRQGRGWNEGIGGLPFVAVAIGVFMAIIYVITVDHKMYMKKVVASGVGYTTPEARLPMCIVGGIALPVGLFWFAWTNSPSVPWAASVVASIPFGFGMVLIFLGIMNYLIDSYTIFAASVLAGNGIIRSVFGAAFPLFTKQMYDSLGIHWASSLPGFLALACIPLPFIFYKYGGAIRKKCKYAAQADAFMQRIRARAQQQEAVGEDSADSSRAATLQVEEEAEVEAHEEEGAPKFAELKAEKETDGEGLRKIQTGRSMRSKRSVRVGDDFYDNPYEIDRVNTRESFVGRARANSKASTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6G5</accession>
    <name>A0A9W8Y6G5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pru domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_006671</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6G5"/>
    <dbReference type="OrthoDB" id="340431at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2020.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.70">
        <property type="entry name" value="Proteasomal ubiquitin receptor Rpn13/ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006773">
        <property type="entry name" value="Rpn13/ADRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044868">
        <property type="entry name" value="Rpn13/ADRM1_Pru"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038633">
        <property type="entry name" value="Rpn13/ADRM1_Pru_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038108">
        <property type="entry name" value="RPN13_DEUBAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225">
        <property type="entry name" value="ADHESION REGULATING MOLECULE 1 110 KDA CELL MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225:SF0">
        <property type="entry name" value="PROTEASOMAL UBIQUITIN RECEPTOR ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04683">
        <property type="entry name" value="Rpn13_ADRM1_Pru"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51917">
        <property type="entry name" value="PRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pru" evidence="7">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="152"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="401" mass="43175" checksum="D5DF1B33E9D7DD6D" modified="2023-11-08" version="1">MSVQPLITFKAGQCDLTRGDGTKQTVKPVQTPGYVYLYQGEDEFVHFCWRPRNKSLDESELDLIMIPGDGSFLPYTGKESVEDSENVTSPTDGRIFVLKFNSSSQRYLFWLQSKSQHPRGDASWFSERDLKIGQIVDLLLNGEEIDLQGELAGVQNPPAGDGEDETMEDVEGNDHSASRDRHGSTGGAGAGATGGDVREEGEESREGGADGGRAAASGNDVSAIVADFMNSLKSGNLGSGSQQQSSDSFTTLLDLLWPSHTIPTVEKGSEELIDALCAQLPTTPFLIEAEIEDVDQIDPNSETAQMAIQTLDQEQKIDVLKRILRAPQLRAALGSLTEALRTGALPTVSQALEIDVEDGGFMRGGAMPLGGGDAVKAFLEGVKKTVEKEQKGDDEDEMDTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6Z9</accession>
    <name>A0A9W8Y6Z9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_006319</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6Z9"/>
    <dbReference type="OrthoDB" id="2011998at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034431">
        <property type="term" value="F:bis(5'-adenosyl)-hexaphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034432">
        <property type="term" value="F:bis(5'-adenosyl)-pentaphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008486">
        <property type="term" value="F:diphosphoinositol-polyphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000298">
        <property type="term" value="F:endopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901911">
        <property type="term" value="P:adenosine 5'-(hexahydrogen pentaphosphate) catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901909">
        <property type="term" value="P:diadenosine hexaphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901907">
        <property type="term" value="P:diadenosine pentaphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071543">
        <property type="term" value="P:diphosphoinositol polyphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04666">
        <property type="entry name" value="NUDIX_DIPP2_like_Nudt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000046">
        <property type="entry name" value="Nudix/MutT family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047198">
        <property type="entry name" value="DDP-like_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12629">
        <property type="entry name" value="DIPHOSPHOINOSITOL POLYPHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12629:SF0">
        <property type="entry name" value="DIPHOSPHOINOSITOL-POLYPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="5">
        <location>
            <begin position="24"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4369234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="161" mass="18424" checksum="C11CC13D7B536507" modified="2023-11-08" version="1">MAATQQLPMQSRTGRVNQRYGAQGERLVAGVVPLSADKYYVLLIQSTRRGGWVLPKGGWETDEATAQDAAKREAWEEAGIICKINYDLGLIPEKRKPDQLTTQAPKASYHFFEATVEKQETQWPEMHKRSRNWFSYTQARQALAERPELLDALDRCTMHRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y713</accession>
    <name>A0A9W8Y713_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y713"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="360" mass="39583" checksum="926580656FA7CF49" modified="2023-11-08" version="1">MSQPLSQERMWKQRAERGLRYQKQRGSRDLRADFRAAEDENVPLSVPQDRISKGRAERGSLGLRDRGSLELLAAFRAGGNMNEKAVPVLSGKGLTHEEHVIETSSEQVQEDGMVGDAGGYEEFNRPHFAGCLGVDTDPSQKLVFVEKNVFDEAFGSAALNPAGRLEVASRPRPESVTTAPALLKREQCNRDSCTSDLYRNAQIYQDGEKNWFMALPTSLLSKQQNHNRQPIPSAIPATLRMSIEEDDAPKPTTADAPILLLDHDDKVSVGRQLAQQYVQYTEEFPGDRGKIQSSIDQVEWTAGYPRLAESDSGSDDSGGLTRMVSGIRGRLHSVGRRGSVVKAGMKASVEAVSGVMCLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y791</accession>
    <name>A0A9W8Y791_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005369</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y791"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="53"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="55"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="251" mass="27305" checksum="8A9743DC4130DA70" modified="2023-11-08" version="1">MSQPWDYIAKIVSLGDSGCGKSSVLRLCEGRFSPHHDVTIGVEFGSRIVPVGPPASQSLSINNPTKAPSETSPSSKQQPSKQQQQQQPQPQKHMKLSLWDTAGQETYKSITRSYFRGASGALLVFDISRKNTFLSATSWLHDLRQIAEEGIIVVLVGNKSDLASSSTISDSEAANKRQVTVEEAQEWCKANGVMQYVETSAKSGDGVERAFLEVAERIYQNIEAGKYDLNDRRSGVKGPGVEGEGGRGESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7J7</accession>
    <name>A0A9W8Y7J7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Wbp11/ELF5/Saf1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006238</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7J7"/>
    <dbReference type="OrthoDB" id="5597581at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019007">
        <property type="entry name" value="Wbp11/ELF5/Saf1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09429">
        <property type="entry name" value="Wbp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Wbp11/ELF5/Saf1 N-terminal" evidence="2">
        <location>
            <begin position="4"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="323" mass="36249" checksum="1486EB0B6A8AEB21" modified="2023-11-08" version="1">MAKEKNYNPVQEAKKAEKQKQIKKQKANLQAQRNEKLARRNPNRIQRDIDSLKELEQSGSIRPHEKQRLQELEKDLAGVNKARAALGDKAPVFKPERRFDDDGSRGGRGGRGGFRGDRGGRGGILGKRTRDGQRKEDESSDTDADVRDIPMPRDTPPPIPRRYQQTQPQPEDEAPAELKKPTVVYEAAPERRDFLKEATKFMPASVAQKIKLAKGEGRLLEPEEFDKLKDEGYMDTSTTKLESETVAGQTAETDEELERFIKEQGTSAKDAAGQAVEAAIQEAEYDMMAAEVRGEIVTAGEEAEVAEKKLRHVEIEEVEDEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7S2</accession>
    <name>A0A9W8Y7S2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_005331</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7S2"/>
    <dbReference type="OrthoDB" id="5215911at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF149">
        <property type="entry name" value="TRANSPORTER, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="420"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="457"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="487"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="45"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="238"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="250"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4369569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="532" mass="58935" checksum="8964952FE6C72B98" modified="2023-11-08" version="1">MPHNDNVELIPGTEIMNDDGTAKKTLIPRPSSSPQDPLNWSTTWKLTVMASQWLFTWISVTGALSIAPMFPLLGQEFHLNNNQLSMLTGITVITLGFANFIIVPLSNIFGRRAVSLVFSILIMLTCIWQALASSHRSLLAARAVNGLVCATSESIPVQMIADVFFLHERGLWTGVYFTGYFMGAFLGPVMAGSIAAHHGWQSFFWLETALSGVAIILVAVAFPETRYHRNHAARKSRAPIEVTSSDDEANMEKQVTHAAAHESESSSLEASPLKNGRPSRAQYMPFRKPDLRWKMFVVRDTWTPIKVFFNPIILWAGLMLAGPADLVLFFNITESPVLAAPPYLFTPDKVGYTNFAFVVGALIGLATAGPYSDWIAARATRKNGGVREAEMRLPALWPYMAITMLSLILGGVAYQRQWAWGHIVVWGYGLAGLSVTTVPTICIAYAIDCYKPISGEIMVVATVCKNFIAFSYSYWVFDLAHESKDGWITPAMVIFACTVGPALFAFPLYYGLGKRIRRWTKGSDVHRMEEMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7W4</accession>
    <name>A0A9W8Y7W4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Beta-xylanase</fullName>
            <ecNumber evidence="10">3.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_005439</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-xylosidic linkages in xylans.</text>
            <dbReference type="EC" id="3.2.1.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan degradation; xylan degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the glycosyl hydrolase 10 (cellulase F) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.8" evidence="10"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7W4"/>
    <dbReference type="OrthoDB" id="3055998at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031176">
        <property type="term" value="F:endo-1,4-beta-xylanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044846">
        <property type="entry name" value="GH10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001000">
        <property type="entry name" value="GH10_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31490:SF35">
        <property type="entry name" value="ENDO-1,4-BETA-XYLANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31490">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00331">
        <property type="entry name" value="Glyco_hydro_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00134">
        <property type="entry name" value="GLHYDRLASE10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00633">
        <property type="entry name" value="Glyco_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51760">
        <property type="entry name" value="GH10_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0858">Xylan degradation</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040907398" description="Beta-xylanase" evidence="11">
        <location>
            <begin position="19"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="GH10" evidence="12">
        <location>
            <begin position="29"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4369677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="356" mass="39745" checksum="FB580D999DB192F1" modified="2023-11-08" version="1">MHLSNIIATLALAGSVIAAPARKDDCKDDSLYKAMKKAGRSFIGTALTIRNETREGEIIKQEFNSFTPENSMKWESTEPSRNNFTFEDADRYAAYAKKNNLQIHCHNLVWHSQLPEWVSNGGFDNKTLISIMENHIKTLVTRYKSVCTRWDVVNEALNDNGTYRSSVWYDTIGEAFLPIAFRLANKYDNKAKLFYNDYNLEYNDNKTVAAKNIVKLIKSYGVKINGVGYQAHLASEVTPTAPGPAPDQKTLEAALHATADEGVDVVYTEIDVRMNTPATAEKLAVQAATYERIAASCLAVKRCIGMTVWGISDKYSWIPGVFTGEGAALIWDENYNKKPAYNGFLEGINSGKKHWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y853</accession>
    <name>A0A9W8Y853_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PinX1-related protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PXR1</name>
        <name evidence="11" type="ORF">N0V83_005009</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in rRNA-processing at A0, A1 and A2 sites and negatively regulates telomerase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PINX1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y853"/>
    <dbReference type="OrthoDB" id="29523at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050656">
        <property type="entry name" value="PINX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149:SF31">
        <property type="entry name" value="PROTEIN PXR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="G-patch" evidence="10">
        <location>
            <begin position="25"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="155"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="155"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="178"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="272"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="304"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4370488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="362" mass="39626" checksum="73422509A0BB448D" modified="2023-11-08" version="1">MGLAAPKNRSKISNDPQNTTWANNTTRFGHRILTSQGWQPGDSLGATDASHAAHYTAASQSHIRVFLKDDNLGLGAKRGSERAENFGLAGLESILGRLNGKEAEVKKEEERREEIEKRAFVYRKYGMMNFVSGGFLVGDKITKSADIKKELEVKVEIKSEPESDDGKVDKKSKKRKREERAGRDAVEERTAEEPVLKRKKKSMDLREEARKEGSESSIAKKEKKSKKAKASPSSDPEPETSGAPTPVSDPEPLTDKARRKAEKKARKEEKKLKKALKKAAKKAAKSNGDDKDTSSESEGEESTPGASAPSSVSATGTSTPVLTSAGLTFNATNARGMHAVRQRWIRQKKAATMDAQAMKEVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y868</accession>
    <name>A0A9W8Y868_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005543</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y868"/>
    <dbReference type="OrthoDB" id="6493944at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42345">
        <property type="entry name" value="TPR_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42345:SF1">
        <property type="entry name" value="VTC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="810" mass="91507" checksum="58F6874CFE94C63F" modified="2023-11-08" version="1">MPTRVSRSILSEHCYAEAMNSEFARWDVTKHQHQYNSKWGVHFPEAPPAPCPLPYAFPEQPFPQLTDGFAQLQVGKIPKTRTTPVTTRTRITRHGSQPNPVRPSSSGGEAKLTSYRPFAPARRSESYSHPTPLEDFRNSRRWPPNGYRQDDALNPQEAEDLICAGASTFKGYWDVVPNAPAGYDRFYAVMESEHQSRTDRAPFQQQAYRDISLNMTGASDSQFPWLSQEQPCMAYAFGKSAGTTTLNYWVSKSGSSNPPTKFAGDAKPRKIKLLQILDRLQRLEDGLNEDDPEEMYRYLYSTLIEDPERFGDQRHIRIDQQIADLITVLSSPKWIDFSLPKNQVVAKFFDAPDQHKKQEFYHQLLLSVELYLRIHSGDHTDKSKQKIIMQLPSIITWDLAVAQRWLENMEITKSRISSSQSTFSFDLKSKHRQKEALRFFAATLKWPNMDEIEYVLEEQDVKEKALEDRSADAMSWFSGIILPGRTLPWLIMNSLIDCDRDTGDTLKYLTHMQPASGFQYRANTYWSTQCIVGKVLGAARGVKQIAGWIGPCIYTPDLKRTECVRVKQHEALDPKLASTDVESMKERTNPLGPIETSYPVDDYEVPMPDTEDVTDLIRMEKLSFAPAKEQPASTRHLPGAPLLFDAAIFFACGGESWPMKLRYDVSFINAFPCHQGPHVLFYDFAYKAIKIDDGLVDIHDWGRQSGRISRPTSSRSSPGKKALALTNGHIKTAHEQVTNVLAIEALGVSDNEVFARAWCAHHGHSAIVANIKETCMACAIREAYAACVSVVILTEGGIVKENDTGIYGYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8F9</accession>
    <name>A0A9W8Y8F9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribonuclease P protein subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_006662</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8F9"/>
    <dbReference type="OrthoDB" id="124041at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="entry name" value="Ribonuclease P/MRP, subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016848">
        <property type="entry name" value="RNase_P/MRP_Rpp29-subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027081">
        <property type="entry name" value="RNase_P/MRP_p29_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="Rof/RNase P subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="34"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR027081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4368306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="232" mass="26535" checksum="18A74725D046FEF1" modified="2023-11-08" version="1">MTDLKGTPFAQALLERAFSPDTAASHYTERVIKRPLNIRPTSPTPSARAIRRQSLNERKQQQQKRSKNKPRPLSAAQKRKLGLNEIPKEQQKYAIYEPLHNLWLGYMREVLGFNDTGRAVYVTPNASGQMLASADMHGALLSVVRSRCVSRVGLQGIVVRDTRHTFEIITKQNVVKSIPKEHTVFRFMVPLPAKEGEEAPKPLEFEIFGEQFQTRAPDRANKKFRMHYQPDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8I1</accession>
    <name>A0A9W8Y8I1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_005790</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4370026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8I1"/>
    <dbReference type="OrthoDB" id="128867at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052254">
        <property type="entry name" value="CUL4-DDB1_E3_ligase_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44472:SF1">
        <property type="entry name" value="DDB1 AND CUL4 ASSOCIATED FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44472">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101908">
        <property type="entry name" value="Putative isomerase YbhE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="370"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="383"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4370026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="510" mass="57057" checksum="02C9C9F360D02BF0" modified="2023-11-08" version="1">MSSYKGELPGLYWDADKEKYFGIQSRAAARGTDLKYSVENIRKEDKKDRLQKADSARIDKIRKQRVVRRHASALLRTGVERELGSKRTSFYLRNLLPQACMSQVSAKPSQVIARPDASIIRYFDRDPGSKTLYVVQGDNTVKARRIHTNIGPPLPTQNTTEANDFDSSTLNTHSFLPWDDVARTTSTVSSLNCLPVTGALATTTYGSDRPPVVYFNDPERDGPFIGQQFTPPNCTTIWGAAARPTSFSPSPRLANSVAATQTEHLAVAASTRVLLFTHAPTGTWDFRVVVHTVKSDILALDWISRNTIIFGSRDGKIRLHDTRSGGSSHIFTHPYPISKLKRADDDTRLICSGLQDSLFLYDIRSPRLSHHPSASRDYRASNHDYSNPNYFTSQGTNKRKRLDRDTSKSWSQPVLSFTHQNEADLELDIDVHPRLGLLAAAQDTDSGSAIRVSNIWTGKTVKTIKSNWRVLSARKRVGNIRSLKFMEKDGEEDGVDLWACWAGGVSKFAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8N4</accession>
    <name>A0A9W8Y8N4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004933</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8N4"/>
    <dbReference type="OrthoDB" id="3781687at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="117"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="270"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="160"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="311" mass="35286" checksum="8792A0661CA9EC0B" modified="2023-11-08" version="1">MGRFASLNAETKDLVNCLLPALAASFNLSDPVEIIPEDIRMRAWDCDRRDHIKDKTENDSRNWGVQFLKDLMAISRMKGGNLAEFQADLRSKVALHEAKHPWCRLADIKELKEEYEHPDRKAEVNSEEQDSSEDSYDSYFEELVEPDEPKGQKRGRKPRNHEVYEARVQESTKRRKYANRGRLRRSEEGGFHRTDKSNNKRSSLERVTPSSRRASLRRASRTVHSDRECSIDSAGGTPLLLHPMPAFSVSPGPPGAPVYAVNASHEPLAVQKLQAELEVAEAELKAARLKYQYIQAREEAEKNAKYGGTGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8S6</accession>
    <name>A0A9W8Y8S6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8S6"/>
    <dbReference type="OrthoDB" id="10633325at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="116" mass="12728" checksum="4C4A2E71198DEB9F" modified="2023-11-08" version="1">MRGYTKAADEDVKTTKVLDISKELYGLLAQPREGLSYDRPRKERNGARQRQEFGGAEIARQEGAALVARRLLQSHPDVTLCLPIRFNLPHCFISAGGATGSGNSPFPTVLRALGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8T8</accession>
    <name>A0A9W8Y8T8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_005408</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing (1-&gt;4)-linked alpha-D-glucose residues with release of alpha-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.20"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.20" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5839090at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004558">
        <property type="term" value="F:alpha-1,4-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06602">
        <property type="entry name" value="GH31_MGAM_SI_GAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05251">
        <property type="entry name" value="NmrA_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030459">
        <property type="entry name" value="Glyco_hydro_31_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF133">
        <property type="entry name" value="P-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00707">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="8">
        <location>
            <begin position="625"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="10">
        <location>
            <begin position="1065"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="domain" description="NmrA-like" evidence="9">
        <location>
            <begin position="1280"/>
            <end position="1573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4369646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1613" mass="178003" checksum="96A7AAA998384469" modified="2023-11-08" version="1">MQAAKNTIAENLGKIVPGAHELAPEDSRFSLEEVPDLSGKVAVVTGGSEGIGYGCTHTLLSHNIAKLFIISKSEDVVDDAINAIKEEMGEEKANRVVWLQCDLSDWEQTGKTAFEISEKTDRIDILINNAARGIMTYQLAKNGVDLHMATNHMGHVVLTSHLLPLLKSTAEKGNTVRLVQLGSNLHENAPKETKFESLDELNTDYGPQPQYGRTKLASILYAKYLDRHLHSKYPNILSNAIHPGIVDTRQSAVHIHEAYPLGGYAMSVGLAPFKKTQFEGCVSAMYAATKTEKSGQYICPPAIVEPGSDKANDAQLGEALMDLTWKTVKEKTKSTTQSPTERVIPTLSPQINALPSLTPTIYDESAPNAQNCPGYKALNVAQTESGFTADLSIAGANCQAFGNDIADLVLEVNYQARERLNVRIYPKYIAPANSSWFILPPSIVDQPQWDGNTAANSSDLTFTWSNDPSFQFKITRTRTGEELFSTYGHVIVYEDQFLELVTNMVADYNVYGLAENIHDFRLGNNHTQTFYAVDAGNSVDGNVYGTIPFYQETRYGQNGSDTTAHGVYARNAHGQEWLLRENTITYRTLGGSFDLYLLSGQDDGGSSSALKTISQFHLGCIGTTAMQMYWTFGFHQARWGYENISVVQNVVDSYRAANVPLECLWNDLDIYELYRDFTNNNATYPLPAFTNFIESLHANGQHYVPIIDSNIYVSNLDNASDAYGPYERGDTLQTFIRDPTTGDYYFGDNWPGFSVWGDWLIPSTQAWWTNEILNWHKGTPFDGIWIDLSEASSFCAGSCGNGRLTENPVHPPFLLPGDPLTFDFNYPEGFNVSNATDAASAASASSSQASALSASPILPAVTTTTQGRTEPTPGVRNLNYPPYVINNVQSGHALGKSSIAPNATHNDQYNTTEYEMHNLFGLQISNATYNALLSVFPGRRPFTVGRSVFAGSGKTTSHWGGDNTSTWGSMFLSISQALGMMMSGIQMFGADTCGFAHNTDYELCSRWMELSAFFPFYRNHNVKATIGQEAYHWASVSEAARRAIAVRYSMLTYMYTLMWYAHTTGETVMRALAWEFPNDETLRDTYSQFLLGPSILVTPVLVPNVETVNGVFPGIGEGTRWYDWYTLQEVKASPRENVTLDAQLEHVNVHLRGGSIIPLQQPKYTTTETRNTPYSILVALDDNNFAEGSLYLDDGVSLIPNATRLVVFAYVDDCLSYSSDGDYVAAQPLANITITGLEASPNGHQRKRGNYATGKPQNISCTYGGEQKSTDGLETSYENVIIGITGNQGSSVAATFLRDPSWRIRGLTRDTSSPTSQSLAAQGVEMIQADLHDPSTLQSVFKDASLIFSVTDFWTPYFTPANQARAKELRKSIGRYAYELEYEQGKNIVDAVAKEVDGLDDVGFIASTLCSAKESSGGQYQELYHFDAKADIFPTYVEEKYPDLAAKTSYLHTGYFYTSSKILPGRWFAKLPDGSVQMQFPTDPNVVIPHLNPRTDTGPFVAALLKLPPKSTLMAASEWCTWPEWIALWGDVTGVKTSYKQVSVEDFDEHMPGGAGKEIGEMFDFSSKFGYNASQKDTLKSWDLEKLGIDVPTMGLRAYFESEDWAAAGIKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9L8</accession>
    <name>A0A9W8Y9L8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ML-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_006229</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transient receptor potential (TRP) ion channel family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9L8"/>
    <dbReference type="OrthoDB" id="5377623at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010308">
        <property type="entry name" value="TRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040241">
        <property type="entry name" value="TRP_Flc/Pkd2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032800">
        <property type="entry name" value="TRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_7G01610)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145:SF7">
        <property type="entry name" value="TRP-LIKE ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06011">
        <property type="entry name" value="TRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14558">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01320">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040973656" description="ML-like domain-containing protein" evidence="9">
        <location>
            <begin position="27"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="372"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="419"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="455"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="563"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="589"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="620"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="650"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="ML-like" evidence="10">
        <location>
            <begin position="49"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="898"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="898"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="924"/>
            <end position="934"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4369145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="934" mass="103057" checksum="3CEE41675C0F3B2B" modified="2023-11-08" version="1">MLPRMPFKLLLALWLIALSLAGFALADDPEYVVAIIDGQKMRVRDDRQPALFTADYGDCLGDSAINVTRFDAAYYKDNMTIIFHLAGETALANDAIMMYIGVYAYGETRFELTFNPCNANIDRAGTPIEAAGIIPINQNDVAGIPEIALSIPDFEGQAILRLFSNSTQNEIGCFAAQITNGNTFQQKVAVSSVLGIFTSVAVLSSFATAVYGDEITVIRKHYAHSLSVQVVFAVWHHIYYSGALSMNWPSVLVAFWSNFAWAGGIIYSENMQNTINEFIGANKGNTSHVGAAGAGVQNPTLGGGYDIHQIYKRGALPVALAVQETVAKRHLINATSGFRYYGQPVKPGLPLPGNYSGFSGTLAQDRIPASNAFMTGLLGLLILLSGVAVAITVLKVTMEGLSMTRLVNNDRLAFFRAHYLGYVAVALLRTLFIGFFMMAFLTMFQFSYLDSSGPVAVACVVFIGMVLGLGVLGGVACYYRVTFGEYVSEPDRLNMEKKKVLHFLPWFSVSRESQEPRSEDKVYVTSLPWWTVRSRIGGKSTHTDERDIKYFGWLVSRYRRTRWWFFIVWIIYDFTRACFLAGASDRPTIQVFGLLGVEVAAFIGMICLRPFEGQRLNIVVVYLLGFSKVSTVALSATLDTRFNLPRIPATAIGIIIIVIQGLLTIAVMIAIVAGAITTYISVMRNRQEIRPKKWNLLRDKYFQSMDLRAQDIPRPRASPSPVESMPDVPIGSYFNVKQVKRMAKVEDEDIEFMMEVHNDASVSQRSLVGRERPGLVGLQRDTEASIRSQTSLSSLPKRARLHRANWSLQSYGRFQGPGRLGAIDNTMIIPARSSSLSSQSYPRLNEQLGSFGSLDRHPSSLNARACRSEDSAIPLISHSSSVGDMRCTGKPAVSRLMSRSQSSFKSTLDSRISEEIPVLPEQSGAKRDAVEEAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9Q2</accession>
    <name>A0A9W8Y9Q2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Vesicle trafficking between the ER and Golgi</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SLY1_2</name>
        <name evidence="2" type="ORF">N0V83_004463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9Q2"/>
    <dbReference type="OrthoDB" id="10251230at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="388" mass="42406" checksum="C17A4FA69D07406D" modified="2023-11-08" version="1">MSIPLDKDHPDKGSRKQAYDLTANDFFWKAHAADPFPNVAEAVTNEWTKYQADADSVTKMTGTSSIDDLDGESNQFAAHLKGAMSLLPELRERKATIEMHMNLLEGIMEKIKTRKLDVFFQLEEELSKQTKASMLEIIKDSDKGNVAIDKLRLFLQWYLTTEQELSRGDLESFTRALEEIGADTSVIQYVKTVRQITRMSMISTPAQPAQTSQLFGGFSSISSRVTDKFKEAGLGANFEGVLSGITGFLPKNSDGTLTKVTESLMEPSNASSSAISLTESFLYFDPRSANARGTVPPASQSRNQQSTGVRGIDASFGQRRAGFSEAIVFTVGGGSMDEYGNLQEWAKRTNTGGAAGTGKKRVVYGSTALYSPTTFVTEDLAQLGKEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAB3</accession>
    <name>A0A9W8YAB3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_004743</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAB3"/>
    <dbReference type="OrthoDB" id="6770063at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000297">
        <property type="term" value="F:spermine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140115">
        <property type="term" value="P:export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042908">
        <property type="term" value="P:xenobiotic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF38">
        <property type="entry name" value="POLYAMINE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="38"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4371524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="137" mass="14769" checksum="E6F05991265C52A5" modified="2023-11-08" version="1">MPPPRDPSTTLSSAHLSSQWDRDPRNARNWSTIRKFSTCLIISSIGFTSTLATSIYSPGTSSVASEFHISHTIALLPLSTYALGMGFGPTIASPLSEMLGRRFVYLATTPVFAAFILGSGFCTSILSAGTFDPRLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAC2</accession>
    <name>A0A9W8YAC2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_003910</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAC2"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000064">
        <property type="entry name" value="MFS allantoate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF40">
        <property type="entry name" value="THIAMINE PATHWAY TRANSPORTER THI73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="274"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="338"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="398"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="45"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4372137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="814" mass="89639" checksum="7C3FBDA2F32DD33C" modified="2023-11-08" version="1">MENTSVKPIETASSDHEIKQVDSNSVGQMGYIDQSALYRKIDWRIVPLMFLCYFLQFLDKVVINYANILGLQKDLGMRGQDFSWVATAFFIGYAVAEFPQGFLLQKFPASKVLGFNVLCWGVTLCCTAAVKNFAGLTAIRTLLGCFEAIITPSLILITASWYTKKQSTPRYGIWYCGLGVGQIVGGLISFCAQSGPKNISFAGWRIMMISVGVFNLIVATVVILYLPDSVASAKFLTPDEKTFIAYRISADQSGNGKRIFKMAGLWEALRDLQVWLLFVNTILIGIPSGVITTFSATLIAGFGYTPKQGALLNMPSGVVSIFATLLCTFAVQRGIPRWIGIVALMIPTMSGAGLMSFLPKTNKAGVLAGIYLINFDVAPLALIYALVGSNTQGYTKKIVSTAMVAIAFSLANIIGPQTFRSKEAPGYISAKTEKSNIPFKIYERDSSISSRGQGWAITIHWALPFLKELLSSETLANIDRVQVDPEVGRNDTGNFLFINLQTLEPKFKIPPNERRRVNREKLRKVLLDGVENHVFWSKKLLTIEPATNTKDSVTAVFEDGTRVSGMLIVGAEGSNSRTRKYLRPDAYKNIRLPVRFIGSAVDMTPAQAKPLRDLDPLLFQGCLPAIGTFLWVSMLESPAVNGTLGTDQERYRVQINVSWPLNGAEDEVQESDIKRMAQMKNRAVAFAPCLRKAVEMIPDGSEVLEIVLADWPCLNWNGKGTCTLVGDAAHAMTMYRGEAANHGMLDAYHLTKALVKLHKGELSQQAAITLYEEEMRDRTTTAVLLSRQACLDAHDWDGLNENSAVLKRRAISSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAE4</accession>
    <name>A0A9W8YAE4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">26S proteasome subunit rpt4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RPT4</name>
        <name evidence="11" type="ORF">N0V83_005296</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAE4"/>
    <dbReference type="OrthoDB" id="1664597at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000008">
        <property type="entry name" value="26S protease regulatory subunit 10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000027">
        <property type="entry name" value="26S protease regulatory subunit 10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000034">
        <property type="entry name" value="26S protease regulatory subunit 10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 11" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="170"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4370774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="393" mass="43914" checksum="BC6791874E23E195" modified="2023-11-08" version="1">MSGSSDPEREHALEGYKSKLLESREWEAKLKSLRLDIKGLQHDFDVSEDNIKALQSVGQIIGEVLKQLDEERFIVKASSGPRYVVGCRSKVDKVKLKQGTRVALDMTTLTIMRMLPREVDPLVYNMSLEDPGQVSFGGIGGLNEQIRELREVIELPLKNPELFLRVGIKPPKGVLLYGPPGTGKTLLARAVASSLETNFLKVVSSAIVDKYIGESARLIREMFGYAKEHEPCIIFMDEIDAIGGRRFSEGTSADREIQRTLMELLNQLDGFDYLGQTKIIMATNRPDTLDPALLRAGRLDRKLEIPLPNEVGRLEIMKIHSAGVATDGEIDFESIVKMSDGFNGADLRNVVTEAGLFAIKDYRDSINQDDFNKAVRKMAESKKLEGKLEYQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAL4</accession>
    <name>A0A9W8YAL4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">GABA/polyamine transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">GPT1</name>
        <name evidence="8" type="ORF">N0V83_004034</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAL4"/>
    <dbReference type="OrthoDB" id="4476201at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF29">
        <property type="entry name" value="AMINO ACID TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="317"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="367"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="392"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="429"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="465"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="514"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="519"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4372260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="542" mass="58628" checksum="9771572B737057F1" modified="2023-11-08" version="1">MATTTTTGRTSLTQRNRSIADEPVTSVLPDGDVPYDPAHSFNPDEEVLAALGYKSEFKREFSLFTTFCVSFAVLGLLPSFASTLFYGMGYADAAAVLAPPGWGPFAAWLTGWSNWLSQVTAAPSVNYGISAMILAAASINDPNYVPTDYQTFLLTVFIMILHAILSSMPTKWLAQFNSAGSTFNFVALIIVIIMIPAGTNRASQGLPRFSPSSEVWGTIYEGTSFPAGVSVLMSFIGVIWTMSGYDSPFHLSEECSNANLASPRAIVLTSATGGIFGWFLQLVVAYTVVDIESALDSDLGQPFAAYLMQCMTQKMTLAILSLTIIAGFSMGQGCMLAASRVTFAYARDDCFPLSKYWKRVNKHTQTPANAVWFNCAIGILCLLLIFGGELAIGALFSIGAIAAFVAFTIPIFIRVFFVGNRFRPGPWNLGKFSIPIGTVASAFVLLMVPILCLPSVTGADLTVDAMNWTCVVYGGPMFLVTIWWFVSAHKWFKGPKVNIQHMMLGRDDNVVMGQGPSVSHEEEDRDSSSGEAKIVGEKVSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAU8</accession>
    <name>A0A9W8YAU8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribose-phosphate pyrophosphokinase</fullName>
            <ecNumber evidence="5">2.7.6.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PRS5</name>
        <name evidence="5" type="ORF">N0V83_003269</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAU8"/>
    <dbReference type="OrthoDB" id="413572at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000063">
        <property type="entry name" value="Phosphoribosylpyrophosphate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000030">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000047">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF36">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="4">
        <location>
            <begin position="6"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="106"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="157"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="117"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="163"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4372978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="448" mass="48542" checksum="BA73CB46E873F0D5" modified="2023-11-08" version="1">MVRNIVVLGGNSHPQLTETICNHLGIPPAKVLLDKFKGGETRVEIEESVRGKDVFIIQSGGGKVNDHLMELLIAISACRTASAKRITAVLPLFPYSRQSDIPYNKTGAPLARVPGTRSRTGTYTFDSRPQTPHPGQAESAGLTNGTAALHNQLSRMKMDERNGGSSNPSSPVQQNGIKRTETIDSSKSDKSDKSAHLNSEHGSNSASGTNGLQRQSTTKPPAFEPQVGYRQWVAQAGTLIADLLTCAGADHVITMDLHDPQYQGFFDIPVDNLYGRHLLRKYIQFNIPNYQQAVVVSPDAGGAKRATAIADALGMPFALIHKERRPTQINDRQNATMMLVGDVTDRTTILVDDLADTSNTITRAAKLLKKEGATEVFALITHGILSGDAIDRINASALDKVVVTNTVDQADHKARCPKLEVLEVGNVFAEAIRRVHHGESISVLFDYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB19</accession>
    <name>A0A9W8YB19_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Malate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003389</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB19"/>
    <dbReference type="OrthoDB" id="1859733at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021851">
        <property type="entry name" value="DUF3455"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35567:SF1">
        <property type="entry name" value="CONSERVED FUNGAL PROTEIN (AFU_ORTHOLOGUE AFUA_1G14230)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35567">
        <property type="entry name" value="MALATE DEHYDROGENASE (AFU_ORTHOLOGUE AFUA_2G13800)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11937">
        <property type="entry name" value="DUF3455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040853426" description="Malate dehydrogenase" evidence="1">
        <location>
            <begin position="20"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="25454" checksum="88345EF31A49B859" modified="2023-11-08" version="1">MLFSTLSTALVFFAGSVTALPWPGQPTVYAPATDLSNLAKLFPQSALPAPTEQLKYVLLGIGTQNYTCATSDDSSVPGTTGALATLYDIGSNLNKDPMAKWKLGSIAPLALALSNAPKTLEFSLTSQGYQNVAGHHFFQMISGANTPVFSLDKLQIQPQPLAPVSKLNATDAPAAACPGNNGLPAVQWLYLKDTKGAAQGGIDSVYRVETAGGNKPATCKGMKPTWEVKYAAQYWIFGPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB43</accession>
    <name>A0A9W8YB43_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">TFIIH complex serine/threonine-protein kinase subunit kin28</fullName>
            <ecNumber evidence="16">2.7.11.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">KIN28</name>
        <name evidence="16" type="ORF">N0V83_005029</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.2" evidence="16"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB43"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070985">
        <property type="term" value="C:transcription factor TFIIK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07841">
        <property type="entry name" value="STKc_CDK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037770">
        <property type="entry name" value="CDK7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF0">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="68"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="372"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="376"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="407"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="74"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR637770-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR637770-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4370508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="421" mass="46237" checksum="1571F097CA46F337" modified="2023-11-08" version="1">MASSVVLSPRPNPPGARLAPNANKARGLALLNNNNANKATDAPAAVTAPGDGGLDLAEQMNDEVRRQFVTGAKLGEGTYAIVTAGHYRHDPSKLVAIKKIKFNAEFKDGIAPDAIREIKFLSELSHPNIIKLHAVFSTKEQNLSLVLEHLPLGDLDGLWKNKAVTYGGADIKAWANMMCQGIWFCHENFVLHRDIKGSNVLIAADGTLKLADFGLARSFADPGRKMTTLVITRYYRPPELFYGCSHYGGTADMWSIGCVIAELTQRQFFLPANTDMEELALICDTFGTPTEETWPGVSTLPYYVSPGEHFGAELKGKGRSAAKMAKPMSWWRAKFPLLGEDGLDLMRGMLTMDPQRRLSARQALEHKYWTNLPRPTKKENLPKEGGGEQKLGEDLKRKGGETPGTGRADKVARKLDFGSMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBP1</accession>
    <name>A0A9W8YBP1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_002514</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBP1"/>
    <dbReference type="OrthoDB" id="2309723at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03046">
        <property type="entry name" value="GST_N_GTT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000069">
        <property type="entry name" value="Putative elongation factor 1-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF9">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="4">
        <location>
            <begin position="81"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4373775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="23592" checksum="F7590FFE5FD50C2F" modified="2023-11-08" version="1">MPPITLYFLQASRSIRTAWLLEELNLDYTVQFSDRVNQKAPEEFKAASGNPLGKSPMIRDGDLTVYESGVISESGKVLPLDRAQRVKVRQWVHAAEATFMLHSLAILYARWNIPSDAPSGTVEAAEKGMSTNVQNDLAWLENELSSSSSSSGKFLCGESVTAADIMMQFSADFILARELGTKGKEYPAIRKWLEVCKGTEGYKRAVEKTGHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC83</accession>
    <name>A0A9W8YC83_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004807</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC83"/>
    <dbReference type="OrthoDB" id="3650630at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="270" mass="30600" checksum="D7997639ED4B34A6" modified="2023-11-08" version="1">MRKLFLVAERLTWEEGLVSRQEKELNESLNWKCNYELGILDPFVEDQEHVLGGKNSQANIFHPYQNEHELKLALNYMFMTAEITEASWALWIGIQLDIERMLYEPDGPTNASAASGSSSERKEPHRLNRLDLTHQKTAKVEMIDLTMSADRASHPAPGPLGLPTPSSSSSVPLPESEETVSSPGHFTDHGNNQFMSNSTIVGPTLEPSNHPSISKRITSYKTLQDFYSLLDRIERTSYVGALFLEAYAEKLRDDTCQKCWFNHNIKLDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCP5</accession>
    <name>A0A9W8YCP5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_002703</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCP5"/>
    <dbReference type="OrthoDB" id="1103324at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11065">
        <property type="entry name" value="CYP64-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050364">
        <property type="entry name" value="Cytochrome_P450_fung"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46300:SF8">
        <property type="entry name" value="CYTOCHROME P450 2E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46300">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="518"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="602"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4373964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="624" mass="70808" checksum="C3B165E344249F3A" modified="2023-11-08" version="1">MALQTFLVAAVLLGAYAVYKYLDTSRRHRVPKGLKPLPGPKGYPIIGSATEVPEKNGFIKFAEWGKEYGPIYQVDLAGHNHVWISSDQISKDLLAKKAAIYSDRPHIPALIDDNRTSAQYLPLLSRNDGHTRQRKFANIIMRESEKALFHRYPELEAKRMLVELLDEPEKYNHALESFISRVTCRLAWGHSEGSDELKQRARELLIGVSPTGALPNKLPFLMALPDWLSPAKAWERRRAKTERTWFQTMQQQVVNDIQSGQAAPSWMKIFLDGRSKWGFKSDLEGAYAVGMHGIAGALTIAAPMQTFCLAMTYYPQYLPMLHEELDRVCGDRLPRSEDRPNLPFLRAIIRECIRWRPPVPTGIPHYLIQDDEYNGYHIPKGSTIHPLEWAIARDPEMFPDPESFNPLRWVEPGWPSYQEPLSQFPTIINSTQFGYGRRTCQGQTVTDEDLLIGIGSIAWLFNIERNAEDVSIPAATSPEKAQHSIGLNEKASISNEELNAGVTTTKEPTMEEKILSKYSYPGSTPADKPQPQPKKSQKAAEPAYKPLEWGDITQKPADPTLDYSILLIAKPIPFKFQLKPRDTERANKMRVLFREGVEKGDYSDTREYWGPNQGRDKPVGWSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCZ3</accession>
    <name>A0A9W8YCZ3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fumarylacetoacetase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_003058</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCZ3"/>
    <dbReference type="OrthoDB" id="411064at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050163">
        <property type="term" value="F:oxaloacetate tautomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.850.10:FF:000002">
        <property type="entry name" value="2-hydroxyhepta-2,4-diene-1,7-dioate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820">
        <property type="entry name" value="ACYLPYRUVASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820:SF100">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G01490)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="3">
        <location>
            <begin position="82"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4374317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="297" mass="32236" checksum="792A82D8226AF58B" modified="2023-11-08" version="1">MAPNWDRLIRFIATDGRELRGEPILPSADFDVGTTTEKTGLKAKVIDVANGDVFDSATKVTDETVTVKELLGPVTTEEVPIIRCIGLNFIKHIQEGGRTLPPFPSTFIKANTCLNDHNGTIVIPKLAQDNQADYEGELCFIISKDAKDVSEADAYDYIGGYMSGNDVSSRKLQRDPQLAGTVPQWNFSKGFDTYAPLGPQIVSTKVIPDPSKLHLKTVVNGELRQSEGIDDLCFKVPYLVSYCSQGTTLKKGTVVMTGTCAGVGYAMKNPQFLKPGDVVEVTLSPGIGTLRNVVEYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD31</accession>
    <name>A0A9W8YD31_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003362</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD31"/>
    <dbReference type="OrthoDB" id="5086500at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040730905" evidence="1">
        <location>
            <begin position="26"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="226" mass="23790" checksum="074C7EB29D98127C" modified="2023-11-08" version="1">MPSPRSILLSTLATALTLTSSTVHAAFGPAFSTGPVGSNSWIREATSTLVLPKAPSGSSGDASLWVGMGTSNGDLIQSIADNWNQNDWSIFAYTLLSTSATTQMPVQADGENAQAADQVTMHYKFDDATGNYTQYVLVNGRQVSTLSTSDGHAMGWGSAVECAEENCGTVAAHKWINTTITLNTADPNYDQTMGKAEGVTGEMSTSDGGITWEVTDIDIPQFTFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD37</accession>
    <name>A0A9W8YD37_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">L-tryptophan decarboxylase PsiD-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_002915</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD37"/>
    <dbReference type="OrthoDB" id="5973539at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004609">
        <property type="term" value="F:phosphatidylserine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003817">
        <property type="entry name" value="PS_Dcarbxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022237">
        <property type="entry name" value="PsiD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067:SF9">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_7G01730)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02666">
        <property type="entry name" value="PS_Dcarbxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12588">
        <property type="entry name" value="PSDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="L-tryptophan decarboxylase PsiD-like" evidence="4">
        <location>
            <begin position="49"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="426"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4374174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="447" mass="50648" checksum="14C1BCC67100F481" modified="2023-11-08" version="1">MKHQERVDNVPEEHRMHRTGNWLPADHRVHKEWLSKIIEKVEDSPKDLHPVLQEFKDLIEKNSRIYLLVNSMFEEIPSKKPYSNDPVGHKQVRDYQHMLALFNHLLTTAPEWSDHKFSVGLVGTPLNAVLDWPMGTPSGFAFFLDPDVNKMLKKVLNAWGEYLSSPASAYVLGNDSIGWFSDHATDDLATVANIGKTSYKFEEMFKCDPSKEHHGFKSWDDFFTRHFHEDKRPIAGPDDDYVIANCCESTPYKVGRNIAKRDRFWMKGQPYSLMDMLDMDPLHEKFVGGTVYQAFLSALSYHRWHAPVSGKVVKAYVKDGTYFSEPLFEGLGDPSAKDISAEGEKTGQGFLTATATRAIIFIEADNKDLGLVCFLGIGMTEVSTCDITVKEGQHVKKGDEIGMFHYGGSTHCVLFEKKVELEGFPNTEDPKNNVPVRSQLAVVKKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDD9</accession>
    <name>A0A9W8YDD9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDD9"/>
    <dbReference type="OrthoDB" id="3693611at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="513"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="585"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="614"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="693"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="906"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="616"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="703"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="713"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="728"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="738"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="763"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="908"/>
            <end position="931"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="931" mass="101099" checksum="F19E4F3C1E0DB966" modified="2023-11-08" version="1">MSRSSTRSALGVTTGNIIKNKEASNDSLHKVAIPNIKFNAGSTDYLHKVAISNIKNGSAKALTKTQKAKLKKQEKVAKNERAKLIAEQQKQEGPRSASTSKSPKPSIPPDGTSSSNVMKKSDDNANSSSLSTISEPGRPVKTTTAFAMLPDEEELSPRAKKILAEKREKELFESGVGVVVKAKTPNIRKHDPIKKGRQALTADDKEKYPVQKAAADLVYKRQNNSIPMKRVPQKQTTRNPIHRRTETTKDASPRGDAAVLGVAGLTEVVLDINWSQDGSHYTSSDRAMTATSSESGNEEAPPLAVSVFLQADDDKPVIPHDTTDTADNLHEHNVPDTSSESEDLPDDKVDEAKIDLPMTPIKLPQITVIAPTPVYNDDVSMEGSNNGFAVGDYGDSPPLRNFPSGFAKATILSPQSKESNGKLLEVPADDTNTFEWAFDQDGMLNKVFKQELDLASDPKVCDQGVVTEVLLPFSTIQNDEQLEKHPNPQQIEMASAETEVWDVAFTLEDNTDDVATTESGPELAKMTSEKPVMEATEPPSWVSSFLSLPNATCYPPALTRDQEHELVMAHVTPLSIPVSKSKLSKAEKKATKASNAATTSPEPPVVEPIVPADPTISETPLDNGNEQVDTETSPLKDDKIALSVESAADNYVAHILALPNGDGQTAAERELHERLVAAHVAPLRLPVSKIRKPKAKKLPPASPVEEPAAPETPIDEVSELTLNDEPEPEQKVAEEHTTATTNCSSSNHDANESRSRGRRSHTSRSSSTTSSQSLERKTHTARTTYLGTASLEDFINTLSFDTTSGTTIKASICEAFATLSADETEAIQGKRPVHLDNMKFNSKYTQRRVKLGAMSLYEFLRGGMAFEGDEGRTNVVDVMRAFRDAARKEDARASEKLVWALEMEMASESRQSSRSRLSSGIGWDSGSGSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEH4</accession>
    <name>A0A9W8YEH4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003683</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEH4"/>
    <dbReference type="OrthoDB" id="3789383at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="426" mass="48520" checksum="69257AF6F81E6E8C" modified="2023-11-08" version="1">MPKLLRDTLNNDAATNTILFGDDIDQTTIQHFIACISPTLRSSLPTHDLVEVTVGGRTSGRTTVRATEIQWTMQKLQDLYMLSQYMGADHVCDMIIDRWHEELHRPTAHLLKTEWGPMEPFDILGFDPTFLNFLAQHDDKGIDFLVGVLIVKGRNGRDLLVDYGLNNWSENMKEKLKTLTAKLECEGVLPLSLEDPDAVCQAYHHHHEHDGQCYRAASKMNRATELPTPLSRSAHDGTPMYQYASSTDPEKGRHLEIQRASTEKEGQKRKRGRDEDDFEPAGNAHMQKKLKAFHEENDNDSDMSNSSKEDLEDSDEEDPRAVRICLPKEYTDARYHSVSMQEGEEEEEDNVVPYNQDVVPPNIDRIRYPGKGMNGTKDSQEAAQKKLELVEKQLELFRAAGYDVDNMHVALPEEDEYSDSDDSQDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEJ8</accession>
    <name>A0A9W8YEJ8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YycE-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002357</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEJ8"/>
    <dbReference type="OrthoDB" id="2338662at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058997">
        <property type="entry name" value="YycE-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22659">
        <property type="entry name" value="YycE-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YycE-like C-terminal" evidence="1">
        <location>
            <begin position="28"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="89" mass="10310" checksum="1825C27AFA680F42" modified="2023-11-08" version="1">MPVLTVLPYHLEFTTQEGHEPGRAPTKDNLLVFYLPEKEEWQEAVGRMQEAGFEAVESWNPYWDAEGKGKTFEDADGWRVVLWNGAWRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEV0</accession>
    <name>A0A9W8YEV0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_003044</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEV0"/>
    <dbReference type="OrthoDB" id="3923077at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF96">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="4"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="154"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="210"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="225"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4374303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="280" mass="30930" checksum="D7E39D3B50406368" modified="2023-11-08" version="1">MLVSIVAGGVFFFVCLFQCVPVSYMWDRSSQSGTCVGNTLIGILAYTYSFFSIISDLTFAIIPAFLIWHLQLKRRAKVALIPLITMGCLASAAVFARLPFLKYFNSPDFLWSTTDIAIWSTVEQGLAITAGSLATLRPLFFLAMQGLGLNTRPTGQRPSNYGLSGSKLAIPASGRSHRQHEKLPPDTYPMGSTVQTQHSDIEAAVQIDDSAKSSSWFTGSFPRPPPKDGKKFEKRQIDNESERSLTERNSEDGMQIMVSKSFYMTDEERASRSSGDGPQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFB5</accession>
    <name>A0A9W8YFB5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001479</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFB5"/>
    <dbReference type="OrthoDB" id="5357734at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37576">
        <property type="entry name" value="DEFECT AT LOW TEMPERATURE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37576:SF2">
        <property type="entry name" value="DEFECT AT LOW TEMPERATURE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4376198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="302" mass="32952" checksum="D86FD115846415B9" modified="2023-11-08" version="1">MEYGNSYTRPSYSRDDSNHQRVESTDDDTVGMLSQAVPSSQYDAYASLNQQMKPADAPGPRRMSATISPLSAQESTPPQTDLANLPPHRFQQSPPASPGFRPRLFGARDSGVVPYQENVAWANSTQFRATNASDAPKPDFGAQIAEANTSTTPSKQYFREARHFREPWRPGSWARLPLKGFGALALVVLLTLASMSTALSLSRGPFFQRAILLTNGHYQANPTFVVLGIIASLTSVIAILPLYYGYWELGRKVSLNPLEIARAFGAPLFNGLDGNVTSTDIEMERGGVVVKYGACERGEVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG20</accession>
    <name>A0A9W8YG20_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Siderophore iron transporter mirB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_001623</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG20"/>
    <dbReference type="OrthoDB" id="4078873at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000381">
        <property type="entry name" value="Siderophore iron transporter mirB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF3">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="373"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="442"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="465"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="504"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="579"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4376340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="614" mass="67867" checksum="E9A8FF35BF7F284A" modified="2023-11-08" version="1">MGFTFRGLATNAMLSRNTDAVDANNKEVADVPAAVQGHTSQHDTPDEKLGSPRTGTLSMDSDEELNKVDTNAEVGVQAVQAATMVWSKRDLILAYVFMWLINFFMAYASGTSSTLSYYVTSSFQEHSLTALTGVISSIVAGIWKLPFAKIMNIWGRPVALCIGVTCYTLGLILMASCNNVQAYCAATTFFYTGYNSIDFFMTVFIADTSKLKNRGFFIGYAASPWLITTWVYGYGVDSVIADGGIGWRWCFGIMAIIAPLICAPLIVMFFMNESKARKQGLIPARPSHGSAVQTVMYYLREFDAPGLLILATGLSLFLLAFNLYSRQAEQWKSPMIICFIIFGALLIIAFGLWEKYFAQVTFIPWPLLKNRTVIFTYTMAASLYTGWYIWDSYFYSLLIVNFNQSVPHATYISNIYTMGSCFICLVYGVALRYYGKLKMYSLFWGAPLTILGVGLMIKFRQPNENIGYIVMCMLFIAFGGGVLVTSEQTTLMAVSKQRDFPALLAVESMVIAIGSAIGSTIAGAIWTGVFPARLLANLPADALENIASIYGDIDVQSSYPIGSPTRDAINLSYGQTQRYMLIAATCIYTLMLVSVALWQNVDVRTMKQRTIGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGA3</accession>
    <name>A0A9W8YGA3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_001808</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGA3"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000117">
        <property type="entry name" value="MFS hexose transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF66">
        <property type="entry name" value="MFS HEXOSE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="393"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="427"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="456"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="39"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4374732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="531" mass="59955" checksum="568DB40CB2DEAD13" modified="2023-11-08" version="1">MVAIAMGAPKGDAAAREDAPDFERVSWRKEPNLRKLYFWACVLCVASATTGYDGMMMNSSQQMTRWENYFQKPDDNRLGLMNNAYNIGSIVSFFIVPFFTQWVGRKIPIATGCVIMIAGALVSTFSTDWKIYLAGRLVLGFGNSFAQMCSPILLTEICHPQHRATFTAVYNCLWNLGALFVAWVAWGTSQADNEWSWRSITLLQGLPSAIQLCFIFWVPESPRWLISKERYEEALNMLAYYHANGDRTNPTVQFEYQEMRDTIRMESDANKNSSYMDFLRTRGNRWRLAILISLGIISQYSGNALFSNYINLVYTGAGITDQNQKMALSGGERLLYLCVSVYAATLIDKVGRRPLFLTATVGMVLSFICWTITCAVYENSGETNSAAGYAQIPFVWIFGVCYAMAWSGLLVAYALEILPYSLRAKGLTIMNITVQAILAVGGQTNPVAWKRLPKHWNLALFYTLWITVELIWVYFVYPETKGPTLEEISKIFDGSDAVAQPDLHQIEKEAHAERLDNVGDEKNPHIEQTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGK4</accession>
    <name>A0A9W8YGK4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_000357</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGK4"/>
    <dbReference type="OrthoDB" id="4161332at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050815">
        <property type="entry name" value="TF_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47338:SF5">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47338">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="393"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="8">
        <location>
            <begin position="137"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="497"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="510"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4377532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="610" mass="69214" checksum="8CFB9F377D6394AF" modified="2023-11-08" version="1">MPTGFSPTNAAAQPLSPSAASSLLETLDPFDLITDEVKNSYLRCSYKWSFHHTPTLLLQIRDRTLEPWLAWAMLALAIRFVKDPPPPFTTQTEASNAFAAHARQMLQSDVENPNISRVQALLMLTGHDWGAGNGRRAWIYLGMAIRLVEVMDLTQELKVPRNRMPTREEFIDAEVRRRTAWTCFLMDSLLSGGKGRKRSLAAADMAIQLPCERESFVFGEPTCTEQLDRSMRMPLVTLAVGEVGIIGSSLRCANIWGKVARWACSEDMKNELPWSPTSQFRQLIYELEDWKRLLPQRLQYDLFALHSHNAVEQGQAYCYMHCIYFMSYMFLHRAYLPVLGPQQNGVGADEAPASYNEHKDSWKNWQKTSRKELVRNSTMVLEMLEEMRTFGVYFLRGLVPWIGFTIYTAVGVMLYSFHFPSGDDDPQWREKARERVIQGCLFLKDMQTQWPMAETWFETMKRMQAYYRSVTGQAPTPASPEERQALRRAMIDYGALQPSPVQKPIDSSKPPDSTIVQTNTSPPQQPTSQEALPAQLPNQNIISPPSAMTTTTLNDLSSSFSWSTLNGIPPLQADDTFSLEAFDSAEIEEMMNNATEEFWASFPGEVGVGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGU9</accession>
    <name>A0A9W8YGU9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HypA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000316</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGU9"/>
    <dbReference type="OrthoDB" id="10004862at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025337">
        <property type="entry name" value="Questin_oxidase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35870">
        <property type="entry name" value="PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G03330)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35870:SF1">
        <property type="entry name" value="PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G03330)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14027">
        <property type="entry name" value="Questin_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="51926" checksum="52EEE18AC9DE0B6B" modified="2023-11-08" version="1">MASGSKVHLEASQQPQFYVKGLTAESAEKTSKLLQVNHEKHHIFFNRSGFHNHIAHHLLTIFALNASPAEIQKGYDVNVSYQRPPEPLKQSIVNDMHEPDRFKTYLGDEKYYHDFLVFFQSEFESKGWQNVVNEYLFAGDERANDMLVRLFAGFLHPIIHLGFGIEFQQPAIIAEGLAQAAVHDNWMAPLFLGCEKAAEANRGENGPKKTIVQLLEECRNDEKLSKAPHWDDGNKIRDGILKRAPEEMIKYASQYTVDEEELEEKTAEMINASVYFTAAAQHPPHQIKFDFFYMHCVNSSIFFSNFLSATNSFLTPATKRRLLEWKVWNDITMYVSRGCPQLLPEEITHYQPKKHDNSGTGGWDDIIKRVNALEDDGHASKLIRALAHGEQACEKHAEDDKKGGFQIKGKDAWLKLGHMAIDSVEAEGPNWVRSCGFDEAWEQIPLRDGEEGAGARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH02</accession>
    <name>A0A9W8YH02_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxymethylenebutenolidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_002431</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH02"/>
    <dbReference type="OrthoDB" id="5440at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030638">
        <property type="term" value="P:polyketide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009959">
        <property type="entry name" value="Cyclase_SnoaL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38436:SF3">
        <property type="entry name" value="CARBOXYMETHYLENEBUTENOLIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38436">
        <property type="entry name" value="POLYKETIDE CYCLASE SNOAL-LIKE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4375345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="282" mass="31613" checksum="49C498686CDF5DB1" modified="2023-11-08" version="1">MLLLVADSETDTPLTIDNGVPGSLIKWAEEGFTVVEIKASALSKGSNVIRTAIEALKQCAECDYDAKVGVVVIYADEKDKAIFDQHTVPTLVHLVGTSSSPTHRTADLITYYYANVKSSKFATPFHHHFHYGTEAVSHTRNLAFLKPRIGGPYFDLEAIWEEHTYYEFADRSVEHTMSTMVQEPYVNHVPTVEVPFTAVVNIRGDRLYHEHIAWDQCTVLRQLGLLPEYLPFPHPLPEGHAVPLGATVQYRVPASGIDIARKMRDKNSVDSNGMFNFKVRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH71</accession>
    <name>A0A9W8YH71_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA polymerase epsilon subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">DNA polymerase II subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">DPB2</name>
        <name evidence="10" type="ORF">N0V83_001073</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase epsilon subunit B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH71"/>
    <dbReference type="OrthoDB" id="10254730at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016266">
        <property type="entry name" value="POLE2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12708:SF0">
        <property type="entry name" value="DNA POLYMERASE EPSILON SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12708">
        <property type="entry name" value="DNA POLYMERASE EPSILON SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="9">
        <location>
            <begin position="447"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="611"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4375796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="656" mass="70454" checksum="8064831B0F538727" modified="2023-11-08" version="1">MATARRPEKPSAVPANPIPSSSPAFGTPVHPINPRRTAPIRAPPALKPAPKTTVLPILLPPATLRPLAFRTFTKKHNLTLTSSALQALAAFVGKHCGSGWREEGLAEKVLEEIAKSWKKCGGQVIVEGENETLRSILKTLEGSMNGGRVVQGSSLSRQSSFNFGPEISARPARPTLDSNTSFGMSSLQVEDKEEDEDLLTDPREWINVIGAFEQPKLIYNVTQKHFEKINSKPSLFPDPSRKTELFRQRYHIVHQRILRNETFQAPTFSTARSATLSRTGSIAISQMNTITPIANLLGRTGSTHLLLGMLTVSATGALSLSDLTGTITLDLQHARPIPENGAYFAPGMIVLVEGSYEEDAGATSSLGGSGGIGGTIGGKFLGFSVGHPPCERRTATLGGAEEIDKNSLAGPAFGWTDFLGLGSQRATGSRMNKIASKLLDEEKAHNVVIASELHLDVPSTLSALRTLLRTYTPDPTDAHPIYPLAIVLMGNFSSKASLAGVPGAGSIEYKEHFDALASVLADFQQLIAHTTLVFVPGDNDAWPSAFSAGAATPLPPQTSNREVWGTGKAKGKEGEVIWTSNPSRLNWFGVKGEMAILRDDVAGRLQRTSIRFNKPEPDVEDEMPYAPSAQQNELEAQDAMDLDLPAAPPTTKTTTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHB9</accession>
    <name>A0A9W8YHB9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000859</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHB9"/>
    <dbReference type="OrthoDB" id="265717at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040765199" evidence="1">
        <location>
            <begin position="18"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4378029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="334" mass="37429" checksum="CE2822E3E546034F" modified="2023-11-08" version="1">MINTCLDLALLCHPVLAFLSIQRPKMQHAQQSKWQYFLFSFLSILAIRYAGTSYFTVTDEVLFDVCHTKDQPNPIASLYPMNATGTLNGTVAVIPISIKLARKLIPPQYGILEHAYRDLLPSFPKGMYPAIIQALHDHEVQAFGYQIPDFTRTGIEFPFVDLLGDNTTSFKWAPSLLMTADHEIALKGASDYGTKTFPSSFEPSCDAYRVVPDAKQPGTTYFSAKSANSGAASVTTLFSPVPEEPFPLSFFKNFTNQPTFADGKTCDNMIRLFNTSVTTAPNRIETVKGTVRANIFPFENEQEWRNVYGLRMDTAFIENNYLPCENFRGYGARD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHL1</accession>
    <name>A0A9W8YHL1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_002494</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHL1"/>
    <dbReference type="OrthoDB" id="3725125at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021833">
        <property type="entry name" value="DUF3425"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11905">
        <property type="entry name" value="DUF3425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4375407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="240" mass="27147" checksum="2365B3307EDAA3B9" modified="2023-11-08" version="1">MTMVVEGMLGAFLQHGWPSMKTWFDTTQSHLLGARVLWWQTSSSHEAATQVPPGNSPTIIQLSQPKYPSILDWVPHPGLRDLLILNYQSYDIDQVVCDMTNAFVVEIEESIPILDTSDPAVNLPSISAGYSYNLMELVERASAFGPMENPNPQELAVMSLLQSTESTKYSSMQHPIHRFKLDPRFFDKYPALYDTNAESKYRPRHPPFVQKSQLPLPFTTDSVKEYMNAVLHAKRSMVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIN0</accession>
    <name>A0A9W8YIN0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MFS transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_000405</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIN0"/>
    <dbReference type="OrthoDB" id="5215911at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000319">
        <property type="entry name" value="MFS transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF30">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04702)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="352"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="421"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="459"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="491"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4377578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="534" mass="58412" checksum="4491A2AF518F934B" modified="2023-11-08" version="1">MAAGLEASTTAVPGTVHLVDLDHSMHARHAGNGDIILDPAPSNDPDDPLNWTPRRKLMATICSNLYTCMTGIAVSTVYSVLVPLSEASGVSVSTLNEGTGYMFLFLGWGLLFWQPFALKYGKRLAYLISVLGAIGTSIWSAYVTSNGEWIARCIVMGFFIAPIEALPEISVTDIYFTHQRGTYMGIYALSLAGSNYFAPIICGFIAEGQGWQWVFYWPAIFNAVTFVFLFFFMEESNYNRKSTPVVPGSDAPSTGLTTEDDKEKVHAVASQSSPSVELGWSTKTFAQKLSLWQPNPGQNMLERAKRSLKYLSWPVIFYAGFSYGSYLIWFNVLNATASIILSGPGYGFKSSMVGLSYVSCCIGVIVGAIVSGRLSDWLTIKLARRNNGIMEAEHRLWPFVLCLVMVPGSLILWGVGAAHQVHWFGLVFAMGCLAFTSCVGVTLSVNYMIDSYHDISGDAIVAVILVRNTMSFAISYGITPWLTNLGYQNCFVSAAFVGMAASSVFLIMVKFGKGLRIKSASKYWKIVNADEAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIQ6</accession>
    <name>A0A9W8YIQ6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTP cyclohydrolase 1</fullName>
            <ecNumber evidence="3">3.5.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GTP cyclohydrolase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">GCH1</name>
        <name evidence="14" type="ORF">N0V83_000089</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">GTP cyclohydrolase 1 is the first enzyme in the biosynthetic pathway leading to folic acid.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP cyclohydrolase I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIQ6"/>
    <dbReference type="OrthoDB" id="4966at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00642">
        <property type="entry name" value="GTP_cyclohydro1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.286.10:FF:000003">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1130.10:FF:000012">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00223">
        <property type="entry name" value="FolE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043134">
        <property type="entry name" value="GTP-CH-I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018234">
        <property type="entry name" value="GTP_CycHdrlase_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00063">
        <property type="entry name" value="folE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006825">
        <property type="entry name" value="PRK09347.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006826">
        <property type="entry name" value="PRK09347.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109:SF7">
        <property type="entry name" value="GTP CYCLOHYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="GTP CYCLOHYDROLASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00859">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00860">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="8" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GTP cyclohydrolase I" evidence="13">
        <location>
            <begin position="148"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="104"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4377265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="326" mass="36392" checksum="D5935865745FEA58" modified="2023-11-08" version="1">MDADTPRPKSALPTHMVNGRSPLSVSTEARDKKERESLFNAIETSNRGRQIVNFDGPNGYFPPRSSAANMGGGSVHDRHEIINGEIDVEGYNEGAVAQTPLATSRAHSPYTQHPTIDFDGLSWPSKGTRGRKEATEEEKAENLKKLSGAVRTMLECIGEDPDREGLRDTPERYAKALLFFTKGYEENLRDIVNGAVFHEDHDELVIVKDIEIFSLCEHHLVPFTGKMHIGYIPNRRVIGLSKLARIAEMFARRLQVQERLTKQVALALSEMLQPQGVAVVVESSHFCMVMRGVQKTGATTTTSCMLGRMRSTAKTREEFLSLINRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH07</accession>
    <name>A0A9W9CH07_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="3">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_010265</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH07"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142:SF3">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_3G09070)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041018901" description="Carboxylic ester hydrolase" evidence="3">
        <location>
            <begin position="22"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="4">
        <location>
            <begin position="147"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4363145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="691" mass="74282" checksum="8DC56E1E126B4519" modified="2023-11-08" version="1">MASINGALSIVSFLFASTVASIPSPQSIGSDLSIIAHNDLYGNTTTRRAASIVLSTPYDYLTATSKCAALGTILWNPDGYAQDFAYLRYLDLDKATDDVGLYWVNGTNKVRCRAMTTQGELKEHPCDNKLPALCSNTATDETRQVAVMSTNNATFIGRRRTQDSAFRFLGIKYASIPARFSHSIYLSPVPGANISALEYAPSCIQSACGTSGTPSCSEDCLYLNIWTPYLPNNKATAGKNKAVLVWIHGGGFVGGTGSDTTFDGSALASRGDVVVVTINYRLSTLGFLALENTPLTGNYGLEDQNRALDWLHGHIGDFGGDKDRITVFGQSAGGASVRALLASPQARGKFSGAIMMSTPQGVGYASTAAKYLTIAEATNLTQAIIQETGCTHSSDELVACLRAVDPRILIGFRNASQSYSGTIASYPVVDGTFLTSNGLPLGPSAPKLNVTVISGIMHDDGSPFSSYAPPNSNISQVLTSQSFPASSILSSPNLFPFPNVGTNETLRIFNLTSRVATDAMFRCLHQSTASTASTNHIFRAIYAYEFDRAYQISEWSPNPPACEAPITPAHPFGDTSQPYYKCHSGELYAVFGTTVSQGRHPRDEDDIPFSQFIVDTWSAFARTGDPNPDKAFLEARGFANTSRVVEEAGRWEPVGTGEKPVRVLSTVVSDEAFREVEQCKVLGQPLEYYDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH26</accession>
    <name>A0A9W9CH26_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peroxidase</fullName>
            <ecNumber evidence="14">1.11.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">N0V83_010798</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 14">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="11 14">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="11">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the peroxidase family. Ligninase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.-" evidence="14"/>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH26"/>
    <dbReference type="OrthoDB" id="2113341at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001621">
        <property type="entry name" value="Ligninase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517:SF48">
        <property type="entry name" value="PEROXIDASE 16-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00462">
        <property type="entry name" value="LIGNINASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 11" id="KW-0349">Heme</keyword>
    <keyword evidence="7 11" id="KW-0408">Iron</keyword>
    <keyword evidence="5 11 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 14" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041016840" description="Peroxidase" evidence="14">
        <location>
            <begin position="19"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="15">
        <location>
            <begin position="14"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="12">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="47"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601621-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601621-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601621-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601621-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4361857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="294" mass="31343" checksum="67F0012E581D73D9" modified="2023-11-08" version="1">MRFHTLTTLLLVPTLSSALHIWPRQSSGCPSVWTDVAKELQSDFAGCNADAHGAIRAPFHDCINNGCDGSLILTDECSHSENAGLSGICSKLKDWTTKYNVGAADMIQFAAATAISACPLGPKVQALVGRKDSSVAAPVGSVPSSKDPVDTILAAFSAKGFSAGDLVALLGTHSVALQFFDDPSQAGKSLDTTPDVYDMTFYKETKAGTAPYSLQSDSLMSNDTQTSHSWDQFANDANAWTRVFTDAWNRFSVVGNDVGSLRDCSSLIPTSSVSRRRQAANARFVAKMSAKFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHB2</accession>
    <name>A0A9W9CHB2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_010937</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6/SMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHB2"/>
    <dbReference type="OrthoDB" id="540004at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003838">
        <property type="term" value="F:sterol 24-C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050447">
        <property type="entry name" value="Erg6_SMT_methyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44068:SF1">
        <property type="entry name" value="HYPOTHETICAL LOC100005854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44068">
        <property type="entry name" value="ZGC:194242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="102"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4361996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="304" mass="33924" checksum="9B01C0EE4911C3B8" modified="2023-11-08" version="1">MPPNSTPKSRTFRLRYIGGFLVLLASILVGFIASTEDPEPSVEAYYGSLESRLGYWLVLGNTRHCGLYAKGQLSPFPISTAQRAMEDKLYTRLGLKPGDRVLDAGAGSGYVAMTMAKHGLNVQAIDITPHHVADARKNVQKYGLQDKISVDYANYHDLSAFPDASFDGVYTMETFVHADDPIKVLNNFKRLLKPGGVLVLHEADFGRNSDKLQDVLRLSHCQNTLVEGGYEELLQKAGFEDFSLEDLTDEVLPMWRLFGILGYVPYQIFKLLGIQNRFINVMAGVEAWLNWGDGRYISVRAVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHE1</accession>
    <name>A0A9W9CHE1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_009923</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the THADA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="73997at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056843">
        <property type="entry name" value="THADA-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056842">
        <property type="entry name" value="THADA-like_TPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019442">
        <property type="entry name" value="THADA/TRM732_DUF2428"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051954">
        <property type="entry name" value="tRNA_methyltransferase_THADA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387:SF0">
        <property type="entry name" value="DUF2428 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387">
        <property type="entry name" value="THADA/DEATH RECEPTOR INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10350">
        <property type="entry name" value="DUF2428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25150">
        <property type="entry name" value="TPR_Trm732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25151">
        <property type="entry name" value="TPR_Trm732_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26523">
        <property type="entry name" value="Trm732_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like TPR repeats region" evidence="4">
        <location>
            <begin position="317"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2428" evidence="3">
        <location>
            <begin position="702"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like C-terminal TPR repeats region" evidence="5">
        <location>
            <begin position="940"/>
            <end position="1094"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4363627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1680" mass="186075" checksum="ECE7604B770AC9F6" modified="2023-11-08" version="1">MAEVALTMNLPVPDKVLRDLARNVTKALPNFAEREPAQGVKELQDVIFPVLETADLPELDSGHRAAASNALCAIIEKCQATGVDYVHDAILDDSVWVRLLNIYLQRSDDAKGKSMRQMLLVLTAVVAKDPSKRAFDLRKHASTTFLDIICQRQDRVKVKPALQGLAHFLLRDAVAIAQLTEIYGDLQQRASVKGSDSTSQQNIFKAFLAWIVHHDTALSAGHLVKNYLLQARRQPGSSPQAHDTASSPLWIEPVVQTLRDWPDRILEFKTHVFPHCFLPNIEEYTRFLSYLHFSTHVVPQGTLPDQLRASGHVDNELSGPEEFKILLAAIEAGKELTIIRDVDHRICKDIKVYDGAIHIPDVVFGGWMSLPEPEVRLAGMFLSVYSASVTRPITGGVFKSLQRNLVHLHTETDANFRREVHGYTQKLFDRLRASTATLTKAKFKRGAFPLSRLPFPDGFSNYGALLRKRRKQDLLTEALDFVVWYLRFLEWEMRPTASYQRRITALRSLTIVLRSGIDSGVPQSHLSKSAQGQLPWAHGLEIGNAKLIRVLLDLILDPFDDIRDSAISVLQLCLVALPEARKDAAVATIPRFLSRAETAMLRTGRADQADGVARAYGLIFSLARKSRAGLPDRRFSSKLGVFEHLKEQLDSTLDVAQNNLSKAINGRPVHGIYAALRYIVDQNDFYTELSAAPSETYGKWIQLHQDVVTSIEKLWSYVYHVLCADAPEGHIPDEMEEEASLDTKEILSYSWRGLKEASTLLRTVISKAPIGDSDGNVITPALFERLGSLCFTQLLELRHRGAFSTVSQTFAAFCRRCVSSDIPALRSLPEMWYQETLRSIQDKAGAITRRSAGIPALMSGIMAAEPQPGGKLFPRAMRELIAEALVEAKSENIEESRLPQVHALNCIKEIFTTSRLSVASEVYIGQGLELAARTLNSNILMLFKALIERLLGSDEAQDWKEHQRAKTSRFSFDDYPSLLSILSDLLDPNGPLKQSIESAPDSSSPLDLHGAEGVFPALQILRQARPPEASLAPIVESVERLLASPHWHLRDMAARTIVSLRSVPELYSAALSLLEDVPKSHNTQHGKLLAVKYMIRKLLQDPAHLTLQDFDALMEELGNFARRCYVASKCPFVRSAFLDVVGTCGMALLRRPETLSILHAWENLTASVSIGPRYALGFSAKNGEALLRASISQVFFIDRIILRDGILDFMLSEKHQGIGEALMLLAIEDPDTCCTALDTLDAIIRIQPSSDLTIPLSLVLAHIHRIILEATDAEVISKAQSVLTTSLSSSASLKNDFFSLTTEPQSLLTLSKLETQCVHGGPPSNAQSALHLLAFFLDYAFHTYPVHRKLTLEAIARYIRLLRMTIVDTNPFDARVAAVQSMLALEQIWTASTTSKATSPLLLGLICVLYDLLNDDDDEIRDGAARAATNLLRAQQGGDDVGMEQTVPILTAHRLAAFMTSSRAFSSSSSASSQSYLVKHALHRLACSASSFSLSHYTQESLFSTPFSTLFAQERHQDTSLFATEKQNLYKDDATDAVFWSRVLLSLSLTHLPRDILPHIELLGKWATDALTTLTTTATTEPDGALGWTSKAEVFTLGVKVICAAEVVFLLASHASSSGGTYSGGREGKRRADVLIALRKFADVGAQSDVHGLWLERVERVLEREVLGALGRVIRGQFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIN4</accession>
    <name>A0A9W9CIN4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Vacuolar protein sorting-associated protein 51 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008325</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the Golgi-associated retrograde protein (GARP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the VPS51 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIN4"/>
    <dbReference type="OrthoDB" id="203678at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990745">
        <property type="term" value="C:EARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014812">
        <property type="entry name" value="Vps51"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15954">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 51 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15954:SF4">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 51 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="2" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU368010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="261" mass="28896" checksum="1248320A8517CA90" modified="2023-11-08" version="1">MSTIASPRASISVRSPSSTRTSLDSTARPPNARRNRTALREFYGIKNAPKDADAKISEESARTELGPDEDETLTELDAADFNAEAYVDSLLAKEGLKGVLKVEADLISRTDRKSLVYDNYSKLLSATSTIRRMRGNMDPLAPTTHTLGPAISHIAETAASLSSSMQGIQSKPSGLGIDIRIEHDDAAKEDAATKRKQTETVRWVLDTPRRLRDMLEQGLDEDAEHEWEQVTQILDQWKGVAGVQELREECENILNEEEESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ03</accession>
    <name>A0A9W9CJ03_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1761-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008689</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ03"/>
    <dbReference type="OrthoDB" id="2344991at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013879">
        <property type="entry name" value="DUF1761"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40638">
        <property type="entry name" value="UPF0591 MEMBRANE PROTEIN C15E1.02C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40638:SF1">
        <property type="entry name" value="UPF0591 MEMBRANE PROTEIN C15E1.02C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08570">
        <property type="entry name" value="DUF1761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="16013" checksum="859659670775552B" modified="2023-11-08" version="1">MLILHLLDLPPVKPSAIALGTAFNHAVSLAVLGPVFGDAYRRAQQANSKEEYFHTKEAATAAASWGSSILGSGIQAYGVGALINATGTLTYKGAAYLGSLIFFASAAPSIVSQVVTEKRPLDTIAVGAVARVLETVGLSLFLTYWGTRTNPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJK7</accession>
    <name>A0A9W9CJK7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ca(2+)-dependent cysteine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MCA1</name>
        <name evidence="7" type="ORF">N0V83_008462</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C14B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJK7"/>
    <dbReference type="OrthoDB" id="3223806at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050452">
        <property type="entry name" value="Metacaspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48104:SF30">
        <property type="entry name" value="METACASPASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48104">
        <property type="entry name" value="METACASPASE-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase C14 caspase" evidence="6">
        <location>
            <begin position="139"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="26"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="66"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4365841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="433" mass="47483" checksum="3B0B09516660DAA6" modified="2023-11-08" version="1">MSYYPGQQYHQNSYGAPPPQNAYGAPPQQGYGYSAPPPQQGYGYQPSQSPQPGYQNYGQPTPPPQQYGYNQGPPPQQYGNYAPPQGQQQGRPGMMPTGTSNQWTHGNHNAPPPPPQGAQNFGQGAPSGYSFQYSACNGRRKALLIGINYFGQRGQLRGCINDVKNMSTYLNEFFGYKREDMVTLTDDQQNPMSQPTKANILRAMHWLVKDARPNDSLFFHYSGHGGQTKDLDGDEDDGYDEVVYPVDFRTAGHLVDDEMHRIMVAPLQPGVRLTAIFDSCHSGSALDLPYIYSTQGVLKEPNLAKEAGQGLLGIVSSYARGDIGSMIGGATSLFKKAINGDDVRQKNLRTKTSPADVIMWSGSKDTQTSADASIGGEATGAMSWAFITALRKNPNQSYVQLLNSIRDELEGKYQQKPQLSCSHPLNTDLLYVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK13</accession>
    <name>A0A9W9CK13_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Maltase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">N0V83_008086</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing (1-&gt;4)-linked alpha-D-glucose residues with release of alpha-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.20"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.20" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK13"/>
    <dbReference type="OrthoDB" id="5839090at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004558">
        <property type="term" value="F:alpha-1,4-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06602">
        <property type="entry name" value="GH31_MGAM_SI_GAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000001">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000138">
        <property type="entry name" value="Putative alpha-glucosidase AgdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030459">
        <property type="entry name" value="Glyco_hydro_31_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF133">
        <property type="entry name" value="P-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00707">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040723047" description="alpha-glucosidase" evidence="11">
        <location>
            <begin position="24"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 N-terminal" evidence="13">
        <location>
            <begin position="177"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="12">
        <location>
            <begin position="351"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="14">
        <location>
            <begin position="787"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="273"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4366450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1010" mass="111196" checksum="43EE2938448097F9" modified="2023-11-08" version="1">MPRSSIIQASAVVLSSFSALTSAQTPTSSSAGEWSTTLNGTPTSFRSIFTIPASADEGAVLIPNIQDPEAINAQDVCPGYKASGVQQDDKGLSATLSLAGEPCNAYGIDIEELSLSVEYQAKGRLAVSVVPKYLSASNESQWIVPEDLVPRPKAEDSNGDSDLVFDWGDDPSFWFTVTRQSTGDVIFTTKGTRLVYENQFVEFVNALPEDYNLYGLGERIHGLRLNNNFTATIYAADVGDPIDRNLYGSHPFYLETRYFEKSGNNTKRALKQSELHQRSFSNEDKEGAQGSPYESYSHGVFYRNTHGMDIVMKPENMTWRTLGGAIDLFFFDGPTQPEVTKQYQTSAVGLPAMQQYWTFGYHQCRWGYRNWTEMREIVETLRAFDIPMETIWLDIDYMDQYRDFTLDPVTFPPSEVADFFGWLHGNNQHFVPIVDGAIYIPNPQNASDAYDTYNRGNESGVFLKNPDGSQYIGAVWPGYTVFPDWMSTNGVSWWVKEMVEWYKEVPFSGFWIDMTEVSSFCVGSCGSGNVTLNPVHPPFKLPGEVGNVVFDYPEGFNITNATEAASASAGASSQNAAQSTAEVSSTSTTYLRTTPTAGVRDVNHPPYVIDHVQDGADLAVHAVSPNATHANGVEEYDVHNVWGHQIINATYQGLLSVFPGKRPFIIGRSTFAGSGKWAGHWGGDNASKWYYMYFSIPQALSFSLFGIPMFGVDTCGFNGNSDMELCSRWMQLSAFFPFYRNHNVLSAISQEPFRWDAVASASRTAMHIRYSLLPYMYTLFHEAHSTGSTVMRALAWEFPNEPQLAGVDNQFLLGSNILVTPVLEPQVDTVKGVFPGIVDGEVWYDWYSGERVQAEAGVNTTISAPLGHIPVFIRGGSVLPTQEPGYTTTESRKNPWGLIVSLSSNGAASGSLYVDDGESLEPSETLEVSFSASEGQLKVDVQGEFEDTNALGNVTVLGVSACAGEIKLNGETIDGSKVNYNQTSSVLKLSGLNDLTTDGAWKGSWTLSWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CK86</accession>
    <name>A0A9W9CK86_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UBZ3-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_007466</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CK86"/>
    <dbReference type="OrthoDB" id="288987at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041298">
        <property type="entry name" value="UBZ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012462">
        <property type="entry name" value="UFSP1/2_DUB_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07910">
        <property type="entry name" value="Peptidase_C78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51907">
        <property type="entry name" value="ZF_UBZ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UBZ3-type" evidence="9">
        <location>
            <begin position="71"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="39"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="151"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4366936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="527" mass="59009" checksum="E710188728DA0695" modified="2023-11-08" version="1">MTSDMERLECPMCDFTVPMTDEYFLQLHFEQAHTTDSPFVIQDDPESQPPLLPQSPSSKRKHVADTPSSDEEENTVACPEPQCGEVVLLSDYNDHLDYHAAESLSFDEATGKYHSHHSSATMKDPAIARRPDTGLSKDAFLEHNFDTDLPEALKRNESHGRRIKKHSHRKRSNTNSSEKSTLSRSITTFNPFAKLDKTVKPPSKSARLGVSVARTTVRPASANISQKSELGPHAWEDRMPRWLHDQLDAGPKITVVNRIGRDGRLIKQEQVQNETPGIIPILAQLSALDHSVKNAYYCHPSTLHIGKTPKEGGFCGYRNIQMLLSYIQGAKAQGHEEFPGRTPGILKLQDLIERAWDKGINTIGRVQTGGIRDTRKYIGTPEAQAFCLSSEINCAVETFCDSKDHSIEAHESLLAAIERYFLQAAVSDGSNVYKTLLPPIYLQQPGHSLTIVGFERRRDGQCNLVVFDPMYNTSPAMLKLIGRKNIKTARPEVLHAYRRGARQLKKHASFEILMLTATPPLFPAWDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKH4</accession>
    <name>A0A9W9CKH4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Polynucleotide 5'-hydroxyl-kinase GRC3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Polynucleotide 5'-hydroxyl-kinase grc3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 13" type="primary">CLP1</name>
        <name evidence="13" type="ORF">N0V83_006666</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Polynucleotide 5'-kinase involved in rRNA processing.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Required for endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of a pre-mRNA cleavage factor complex. Interacts directly with PCF11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Clp1 family. Clp1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKH4"/>
    <dbReference type="OrthoDB" id="258143at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005849">
        <property type="term" value="C:mRNA cleavage factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051731">
        <property type="term" value="F:polynucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1030:FF:000001">
        <property type="entry name" value="Protein CLP1 homolog 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1030">
        <property type="entry name" value="Clp1, DNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.330">
        <property type="entry name" value="Pre-mRNA cleavage complex subunit Clp1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03035">
        <property type="entry name" value="Clp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028606">
        <property type="entry name" value="Clp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045116">
        <property type="entry name" value="Clp1/Grc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010655">
        <property type="entry name" value="Clp1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038238">
        <property type="entry name" value="Clp1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032324">
        <property type="entry name" value="Clp1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038239">
        <property type="entry name" value="Clp1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032319">
        <property type="entry name" value="CLP1_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755">
        <property type="entry name" value="CLEAVAGE/POLYADENYLATION FACTOR IA SUBUNIT CLP1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755:SF6">
        <property type="entry name" value="POLYRIBONUCLEOTIDE 5'-HYDROXYL-KINASE CLP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06807">
        <property type="entry name" value="Clp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16573">
        <property type="entry name" value="CLP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16575">
        <property type="entry name" value="CLP1_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Clp1 N-terminal" evidence="11">
        <location>
            <begin position="30"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Clp1 P-loop" evidence="12">
        <location>
            <begin position="135"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Clp1 C-terminal" evidence="10">
        <location>
            <begin position="347"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="138"/>
            <end position="143"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4368310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="467" mass="50698" checksum="40783AB1291E4EFB" modified="2023-11-08" version="1">MISLPGLNLASQPVEPSTVPATTVQRTQDLAANTEYRFEVSFSRTLTVKLQSGTAEFFGTELAPSTTYTFQGTKGAIFTWHGCKLEIGGEVESDYVAEETPMMSCANLHFALESLRDRSVSSGSVEMGPRVLVVGPENSGKTSLVKTLTSYAVKSGRQPMVINLDPRQGMLSVPGSFSAAAYSSIVDIEEGWGSSPISGPSPIPVKMPLVYHYGLKDPEEGKVFKPLVTRMALAVTSRLEEDHASKQAGFIIDSSGAISQGKNGAYENIEHIVSEFSVNVLITLGSERLYSDLTRKFSSQNRDPSESVSVIRLDKSGGCVDRSEEYMRSLRHAQIREYFFGKGDETLAPSSQTADFADLNLFNILPGNDNHNAEYRPGDYDVSMAPIYEKVTPSVVAIQNKLLAVTMASPSDRQEVIRDASVRGYIYVADVDEVKKKVKLLSPQPGQTPGNAMVLGSWPEDVPGLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKM7</accession>
    <name>A0A9W9CKM7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007678</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKM7"/>
    <dbReference type="OrthoDB" id="68483at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="332" mass="37387" checksum="45A55F3559F9A55A" modified="2023-11-08" version="1">MRKRRPERIESILGSASRIVQPPLSMRPSVMRKSKSQDTHDRRPIESALTTEGVHHEIQIDDKNRKMPQTMDEMAYIKSGFRTDSLGVRPSPKPAAKSSPDTRHSPSFDGGKVVHDNMSHRPEKSPPSTSSNTLAGSVPSTPIGKGHAHNPLEDLLYLSIGTGEDSAAPEDTYIVSESPSNADLNVYETAYEEEVQRIINQRKDQYNNNRRPTLYLTRRVENLRSIRDNDCIFDEGQDLRGNLKGSFKSFVTKTKEDIEARGELQKLQEGKEGKLSRTVRNVREAKRLVEEARERVKVEERERERERSRSATPIRKDSSGSGLSRSGTPVGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKT8</accession>
    <name>A0A9W9CKT8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007204</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKT8"/>
    <dbReference type="OrthoDB" id="2118965at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38703:SF1">
        <property type="entry name" value="ALLERGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38703">
        <property type="entry name" value="CHROMOSOME 8, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="335" mass="35171" checksum="5CD5739356183A6C" modified="2023-11-08" version="1">MTHIHETIQPVVQKEVIQPNVVHTTVPIHEVHHNKATHHDTTSLPAMTMDQFKSKGGALTGREERYDEFQGVPKNIGSGTAGGFGAVRGLEHKEHHNSKEHARHGDYDEAPNHQHTGAGIQRGGLTNNSTTAGVGGAAAGLAEGHHHDKHHHNDRDLTGSSGGLTGRDNLDRDGRGLTGSSTTGGLTDRDQHQYADRGMGGQGNLTGSSTQGGVLNSSDRHHDGMSSDRSDLNDPARERSIGATGTTESSGGRKGTDGVISGTGKNDLDHRANEDGGLPKALTEDRSKAVPHSASHDNQHDTGTDNTSGSSKGGLMNKLNPKTDANGDGKAGFMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL32</accession>
    <name>A0A9W9CL32_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Vacuolar protein sorting-associated protein 20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">VPS20</name>
        <name evidence="9" type="ORF">N0V83_006831</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL32"/>
    <dbReference type="OrthoDB" id="441172at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1060">
        <property type="entry name" value="ESAT-6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF5">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="165"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="13"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4368474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="211" mass="23615" checksum="772B27DC9BDBE9D4" modified="2023-11-08" version="1">MGNSSSANRISAQDKAILDMKNQRDKLRQYQKRITVLTDREKEIAKECLAKGNTDKAKLALRRKKYQESLLSKTDAQLAQLEQLTSDVEFALVQKDVLFGLQEGTRVLKEIHREMGGIENVEKLLGENEEARAYQEEISELLSGKMTNQDEDEVEDELKALEAEVSGVTLPDPPVAQPQLTSEEKAQMAKDKAAKTARERAAEQASQPMLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL40</accession>
    <name>A0A9W9CL40_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL40"/>
    <dbReference type="OrthoDB" id="3766406at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="37153" checksum="C62FE24E70E24CD4" modified="2023-11-08" version="1">MNIFRRSMSSSSSRSRTSTTASTSDSPTPRSSFESTQSNNDISTSLATIKMKKAPPAPLLNLPIELIQQITSYLDNASAASFCLSSRFICYAVGTNHLSSYIGASKSRFEKRRTIEAVVERAFPGHWFCAWCDKFHAWSASDGPKEEASVALTTAGVGTKGANSTGTKKGRRRDCAEFNSYLHDGSDYVLYYHHVRLAINRALWGPAHGIPLAALAHSSSGMTKIFKTPCPTKLQITPVITTITGNPGEHAHQPHLLLHTTYALILPTSFTSNKRLLPSQIWPSLPTSSRATATFPRRQRPHRPHGGHRQRRTQGVDVPAHPDVRAVSHGLERQLCRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL95</accession>
    <name>A0A9W9CL95_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006458</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL95"/>
    <dbReference type="OrthoDB" id="3886346at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="360" mass="38979" checksum="8029399057849BBA" modified="2023-11-08" version="1">MLVSADRALPAMSDIDKSFAFLQDTIPKWLQDIARIEDKATAMQDQVTKLSASHSPFATRKSDSIESIQPAKLDAILERAAPSHGAQTDPLGTRKRKTLSVLSGRASSPLRYRPKQMVVVSYDGDMQKSFEVLVRAIGTGRNMLRKAKMEARMNELAALAGGASDDEDEVVNEEEEAIVSKITYRPRMSSMRARATSRRGGPLGAGGSITPVALFDTTDKTLEHAQGLCEKAAHLTLRDGDCRKELGGVRTDFNAVLETANTEVAKCKAKKLQGPLGAHLQHNTDASISSMASSHRKHLPPVSTPSFGPPSKGILPDAPLASALTAPKVIEIEVDDDEEDEEEDFVMPPIRLTSRLTARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLF4</accession>
    <name>A0A9W9CLF4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SUN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_006624</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLF4"/>
    <dbReference type="OrthoDB" id="342281at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034993">
        <property type="term" value="C:meiotic nuclear membrane microtubule tethering complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045119">
        <property type="entry name" value="SUN1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911:SF8">
        <property type="entry name" value="KLAROID PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911">
        <property type="entry name" value="SAD1/UNC-84-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51469">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="SUN" evidence="6">
        <location>
            <begin position="544"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="80"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="36"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="87"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4368268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="739" mass="82371" checksum="57C2BBF129704B22" modified="2023-11-08" version="1">MGAPTPRRSTRLSTKVASSVAAESAVTQVTSGGTNLRRKGPLTKVKARKSNAYGASGRVPAAEELSVSATGFAQAFQNQRGDAFARDDEEDEDEGDDIDELGDDAPLMSGALDGNNHRLSPSPFPDAAARAAPGLSFLESEGVTPSDDDLATSVANTSKSFGMNHEGGMLFQPGRQDMSFSSRLASRRTVQAQTNARARVPAQTQAQAASKQPVTPVMLQNRADIERSVDDLIAEEHARVQKEGLAQHQPRQQSEGSRKHLNTIKEWLRNIELPHINEPQWPWKKYLTWALWFLVASTVIGMISSPLSSIELPKSTSQSPGLATALASRISYQYTKLVNFIEPPAPPAEPTDEEQWRKYTGGDDDVMWSRMNKMNKRYTTAFKEVQSTIDELKNELPLYMILRKYDDGHQEITDSFWHALVDKARSNEATPEWNDFLKEIDQKVRNMSGVPRDNDNAPSWDQIVSRDEFAATMEKHYNTISQRVDEKISEAIRGQFDQIKSIVQAEARKTMLDNIRLQSLAQTNLVANYELHLRKPNYFSPGLGAIVEPTMTSATFSDISNQPSRFLRWLVLPQRNPPVAALSDWRTPGDCWCSTPNPELGQAQLTVMLGQRIKPKQVTIEHVPMSMVPRGDISEAPRDVELWVETEEPVTWQYGHQQADCQEGLPGWACLGSFKYNVHAANHVQTFDLGGDSSVPITKVMVRVVSNWGADHTCLYQVRLHGDDATEAYEYGVRLNDPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLL2</accession>
    <name>A0A9W9CLL2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alpha/beta-hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005778</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4370014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLL2"/>
    <dbReference type="OrthoDB" id="3504488at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12810">
        <property type="entry name" value="Esterase_713_like-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040774366" description="Alpha/beta-hydrolase" evidence="1">
        <location>
            <begin position="19"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="350" mass="38873" checksum="7916B3FFC2935D78" modified="2023-11-08" version="1">MKFAISISLLALVSSALSDDSSQQRPITLSNSGGFQIGGKIISNPRNPNQTLSCDHGYMEYFIPWRPRKTSLVMWHSSSTQVWQNRWDGGEGYKDMFLRRNYPVYLWDGPRVGRANWACEPMMYIPTYMDQANFGAWNFGPRWPVWWDNVQFPTKDAAAWQQATSARYVEFDTKANVELHSDAAAIAADSGKVGKDIVYLTNSAGGLRAMMTATKANGTNIKGIVTYESIGYVFPDNVNITEGKGGFGPFVVPLERFKKLAKLTAIQFVWGDHRAENFTSVIESRLAAKLINQYGGNAQVLKLGDDAGLKGSTHIPFADLDNVKVAGLLDDVLEKNGLDEYVDEEEDDGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMS2</accession>
    <name>A0A9W9CMS2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005021</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMS2"/>
    <dbReference type="OrthoDB" id="3365399at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17080">
        <property type="entry name" value="Ubl_SLD2_Esc2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022617">
        <property type="entry name" value="Rad60/SUMO-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11976">
        <property type="entry name" value="Rad60-SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="232"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="439" mass="49178" checksum="73F0031839AC8990" modified="2023-11-08" version="1">MPDAQQDAPAPKKRSFFKRAAWQDAPKKDSDDIFSHADEFSDIVAEQSRRREEEERRAQSQRQRNKKRRKVSLENEEPMSPGSTSGGVLKQNLDNDVPSRIRTPEPPGSVPDSLSARYDSLTRIASSKDSLDRKQSIVIDLDDIDDQETSTHNSNSLAPNATLAPASRNEHRERDMPILSSRRAPTDDHEIEEVLDPTLAALAARARKRAALKAQSAVGTHGEASKAPIAQLFISPEIPDANPLMVKVRIDSTIEKPRQAWCAKQGFPADMTRNVFFTWKGTRLYDSTTIKRLGMQVDKNGNVSVEGDPNIYDDVNVPKVMVQAWTEALFQQRTKEDAAAKKLAAEVPPVVEEREPTPESVLQVTKIRLILKARGRDDFRLSVHPDTDFAHIAQAYKTRLKIDDNQPLTLTFDGDRLSPLDTVADTEIEDMDAIEVLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN11</accession>
    <name>A0A9W9CN11_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">WD40 repeat-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_003884</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN11"/>
    <dbReference type="OrthoDB" id="366230at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010970">
        <property type="term" value="P:transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000425">
        <property type="entry name" value="Cytoplasmic dynein 1 intermediate chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000414">
        <property type="entry name" value="Cytoplasmic dynein intermediate chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF22">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 INTERMEDIATE CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="520"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="24"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="162"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4372111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="715" mass="78049" checksum="5B7BF9B2EABBE7EE" modified="2023-11-08" version="1">METTMQQRRDEILAKKAKLAELKRQRELRKQESASRQSLSGSPLGEVLSPTPRRSDDLDRRTDVNNLINSILGESRPGSTQPGSPAGAGRATRPTSVVSAGQLSSDNEGPQSYQAPSGMVDREMQTLSMGPLATVYEFDGQVTNEVRTEKEVITYSKGVQASIDWSPPKSRGAGGSDTERDESPARSPKSKRLSRRQKERDEEIRTQLRKEIEEEIKAVQQAPQDGSAPQGSDENFPFKTLTNEELTAVENSEDFIGFVERSTKVLERALDQQLGYDILADYALGGVHVDDEDEGYGSSGGKKGRRIKEIAQFWDERWSKKRMISDLGFSTKFPELVLASYTKNPSAPHDPDGLIQVWNLHMHDRPEYIFHAQSDILTAKFSPFHPNLIIGGAYSGQVLLWDTRAKSAPVQKTPLTGSGHTHPVYSLDIVGTQNANNIISCSTDGVVCGWTVDMLSQPQEFLELTSPSPAKTDDMAVTCIAFPQSDPTYFLAGTEEGSVYPCHRYDRAGAKAGVDGRVKYAGHAAPVMSLDFHPAKGQIDLGDLVLSSSVDWSVKLWKVRPPAATGATNPTTLAGGTAQQVHPVLDIAREDLVYDVRWSPTKPSVFALVDGAGSLEVWDLNVDVEVPVQSVKPSDGKRGAFAGLPSGLGYMNRSLNKMAWERNEGRRVAVGGMDGVVTVFEVGSELGGVETRADEWSGVKRLVNRLEGHGEVNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN29</accession>
    <name>A0A9W9CN29_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DHH phosphoesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_003944</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN29"/>
    <dbReference type="OrthoDB" id="374045at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004309">
        <property type="term" value="F:exopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1640.10:FF:000012">
        <property type="entry name" value="DHH phosphoesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.310.20:FF:000008">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.20">
        <property type="entry name" value="DHHA2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.10">
        <property type="entry name" value="inorganic pyrophosphatase (n-terminal core)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004097">
        <property type="entry name" value="DHHA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038222">
        <property type="entry name" value="DHHA2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112">
        <property type="entry name" value="BNIP - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112:SF20">
        <property type="entry name" value="EXOPOLYPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02833">
        <property type="entry name" value="DHHA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040850684" description="DHH phosphoesterase" evidence="6">
        <location>
            <begin position="20"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="DDH" evidence="7">
        <location>
            <begin position="71"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA2" evidence="8">
        <location>
            <begin position="279"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="260"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4372171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="453" mass="51244" checksum="00B3AE43FE027642" modified="2023-11-08" version="1">MKALRTLLPALLLRSRATALPQARPAGQVILDASESPTKSLGVGHFSEWSRATKKQFLVDWQAGKASEWTIVQGNEGGDMDSMTAALTWAYHLEHSSENTSHPIKAIALLQTPSHALDLRPENKLALDNSQMTPGHEDLLTMDELPEDPETLSRKLKGIVLVDHAEPLRKWSRAKILSIFDHHKDRGTAPDAEPRVFETTASCTTVVARQMLDELEQLDQEYHMPHELLELILGAIAIDSGGLTSGKTTKVDIETAERVLKRSNWREDNLEDVMEELDDELSKAQKDIDHLDLRDLLRRDWKGDLIDTPSPRTPTVSLGFASIPYSMDEQILKTDFAELFDWFAVHAAWTAETGVDISICLNKYKKHYKDGEKEKIREVVLYVRDDVRIDQDQADSLFKVVKDALENNDQGIDLVPWHRADELAPRQMVWTHKSNAGRKVFRPIVEEAVMAWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNP2</accession>
    <name>A0A9W9CNP2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF3844 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_003993</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNP2"/>
    <dbReference type="OrthoDB" id="5583277at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053065">
        <property type="entry name" value="Archenteron_Induction-Rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024382">
        <property type="entry name" value="Vps3844_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049205">
        <property type="entry name" value="Vps3844_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36853:SF1">
        <property type="entry name" value="DUF3844 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36853">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21656">
        <property type="entry name" value="DUF6859"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12955">
        <property type="entry name" value="Vps3844_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040817137" description="DUF3844 domain-containing protein" evidence="3">
        <location>
            <begin position="19"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="351"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar sorting protein Vps3844 N-terminal" evidence="5">
        <location>
            <begin position="21"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar sorting protein Vps3844 C-terminal" evidence="4">
        <location>
            <begin position="274"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF21656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4372219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="394" mass="42810" checksum="8359F6897AEA4A86" modified="2023-11-08" version="1">MKLSWGLIASSLCCSASAARPTVSPETARLILAQRLGLSRFHSIEHADTETIRHINAYGGRQQKLFGAEDSDRTRAHVLMWIEDTVQDEATVIINDGSDRSQDFAISHPPPASDNDRLIQDFISQAQSLPEYPDPKKHTYTSSIEIEAVLPSDNKPELYNEYLSVFRASKSDKFDSEEIAKVLGKVINKAAELGFSTTVVLMPASKSHSKRAASPWGTYDLPSKDARRENPEAVLSSNPVPSSSPISKVSDLEDFPVITFADDQGPVRGILPSCFDTLSACQRQTHNCSSHGECKLLHKGRGSGSNQESVDCYGCACVPTVVRHDTGMETKKKVTYWGGPACQKKDISVQFWLFVGSGVVLSFLVASGIGMLYSMGSEELPSVIGAGVSGPVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPV5</accession>
    <name>A0A9W9CPV5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sister chromatid cohesion protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MCD1</name>
        <name evidence="7" type="ORF">N0V83_002224</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the rad21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPV5"/>
    <dbReference type="OrthoDB" id="10071381at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030892">
        <property type="term" value="C:mitotic cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21788">
        <property type="entry name" value="Rad21_Rec8_M_SpRad21p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.580:FF:000004">
        <property type="entry name" value="Double-strand-break repair protein rad21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.580">
        <property type="entry name" value="Structural maintenance of chromosome 1. Chain E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039781">
        <property type="entry name" value="Rad21/Rec8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006909">
        <property type="entry name" value="Rad21/Rec8_C_eu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006910">
        <property type="entry name" value="Rad21_Rec8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023093">
        <property type="entry name" value="ScpA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585:SF69">
        <property type="entry name" value="FI11703P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585">
        <property type="entry name" value="SCC1 / RAD21 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04824">
        <property type="entry name" value="Rad21_Rec8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04825">
        <property type="entry name" value="Rad21_Rec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Rad21/Rec8-like protein C-terminal eukaryotic" evidence="5">
        <location>
            <begin position="536"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="260"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="403"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="446"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4375140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="632" mass="68818" checksum="016EF5BD3AD69D47" modified="2023-11-08" version="1">MFLPEDLLFKSGQLARVWLAANQHKKLTKAQVLQDKIDEDIQVIIRPEGAAGGPLALRLNGQLLLGVVRIYHRKAHYLHDDCNDALWKIKMAFRPGNIDLPAQTHVANPTSLTLPDMITDLDLLAPMPDPALLLSQALDDNLNFGNTTVPDWDNSQFLSGSIEQPRMEAMELGDDDLNLDIGEDLEAPPAFDDGTSIEIGRNAPVERRASEEFHSSVKDLLEDDLNLDIGEEPTEIAHAPEFNLGDDLDISMGGTEMDLPVAPSVAGDAPAQRAPRGESPLSELDEGEASRLAQEVAEQNTTMFEPGQEPEEEESIHQARAKRRRVIGQDAETMISSRQLRDQQDNRDKILKPASFLPRDPLLMALINMQKSGGFVSSILGDGRSQGWAPELRGILSLEVVSRPSQKRKRDSGVADLGTTEDEAAAEGEKTPQLEFEQDEPTLGGADFGLGGGNTTLGGSDDIIQLPDEPAIQLGEEEEDIYSPVPDNFDDTTAPLIHPAEAGPISLGTKHAVHLLRERFGPEAEESESERQKNSIVFQEMLPEARTSRADATKMFFEMLVLATKDAIKVEQPANELGGPLRIRAKRGLWGEWAETAASGELASQAQAAPQIDVDAPAEAIFQGPPEVSVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPX2</accession>
    <name>A0A9W9CPX2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002286</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPX2"/>
    <dbReference type="OrthoDB" id="407617at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="310" mass="33309" checksum="E2C1F360084D6039" modified="2023-11-08" version="1">MTTTMTKVTSLGSPYSGSGAPSVPNPTPATETLDSLENEPTPPMDFAAVAKILGPIQESLNESAEEAMYPSPPINTPEVLSPAPSVHRYQTRVRGFSLIGDRLAQLKIDSRKPPRRDLDVASSNRSAEPSVTGGEEGDVASVGEVLNECGKDKDGLGHSSMSPAPLGSPVGEGSVPIALGSPTVNPRIEEYLSFTSKDLPTKSPAPFFASTLIEKAPSSTKTAHDNESLDSHNTIQSEDGVTEKSNWGAELEESVDRVCMRLEQLARDEYVIDPSKSLEEYYIARLQRLLGSDKRVVPVKIQKIVHSLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQB8</accession>
    <name>A0A9W9CQB8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Prenylated Rab acceptor protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PRA1</name>
        <name evidence="6" type="ORF">N0V83_001936</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the ZPS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQB8"/>
    <dbReference type="OrthoDB" id="4689212at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11307">
        <property type="entry name" value="M35_Asp_f2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000043">
        <property type="entry name" value="Major allergen Asp F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029482">
        <property type="entry name" value="HRXXH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039124">
        <property type="entry name" value="PRA1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39399">
        <property type="entry name" value="PROTEIN ZPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39399:SF1">
        <property type="entry name" value="PROTEIN ZPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13933">
        <property type="entry name" value="HRXXH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040949540" evidence="4">
        <location>
            <begin position="19"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Putative peptidase" evidence="5">
        <location>
            <begin position="10"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00060890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4374858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="299" mass="31761" checksum="89D0049BE35B2519" modified="2023-11-08" version="1">MAGRISSLLLLGASAIVASPLVPRQSGAATVTVSAAEPTSTAWDAGATREYPIHASCNATQQAYIKNGLEETITICRQARDHILRWGNSSEIYQKYFGDAATGEPLGWYEKVVSGDRADILFRCDNIDGNCAQEGWAGHWRGSNATGETVICDLSYESRLPLEGMCMFGYNVANSPANTFWASDLLHRLFHLPKVGEGAVGHYGAEAKYSGVLDLAKEKSTYAGRNSDTLQYFALEVYAHDIAVPGIGCPGTYIATPTASAEATSEATVAASSAVVMPSEAIASTTAAAEACTPHEDHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQD4</accession>
    <name>A0A9W9CQD4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQD4"/>
    <dbReference type="OrthoDB" id="3684683at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="476" mass="54436" checksum="564B0BAE2B7163EB" modified="2023-11-08" version="1">MAPLLDCPDELLLQITQHLDSQSLLKLMRTCRRVRPVAQEALYCSIVVPPSSNDFDPPLACLLRTLIQRPSLANLVREVWFAPRKQYVPVDLPGQTTLERFHPHVDVTMWEPGVVAAIISLAPKLVNLSVFPCGQFPTHSPMVLPKTNVPPVSIIREMFRSRQNRDGLIDIATLQPYQNLKHVRLCTRILEHEWIKLPNLESLLLCHHTQTFWSGNDPPPANSRMSRLELELNFDILLPVSPGTLLQSLQPTRNLQLHGFLLLFPNLKDLLITLYTPPGLSQHNIVPLSFDNLIAKIPATGRLETLNIRILTSVEDVRTFEHIFPLSNVAKLSALRVLEIPQAALGSQEGPINLEDILPVTLEVLTVHRVDDSMLRWIKRIIAERHRLPNLRRIDLYLIGVRSAEYVGLICTYMRSEDMWKLLEVGIEVDIWWRLEEFQTNWQNIGMGRIHREVEEDPGQSRVFIAVERFLAQSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQI0</accession>
    <name>A0A9W9CQI0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Polyamine transporter tpo5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TPO5</name>
        <name evidence="8" type="ORF">N0V83_002116</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQI0"/>
    <dbReference type="OrthoDB" id="10054429at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF10">
        <property type="entry name" value="AMINO ACID TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="308"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="442"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="481"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="510"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4375037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="562" mass="61217" checksum="83A6F0F9F83ED1A4" modified="2023-11-08" version="1">MGINADSKDSRPGTLPVDGRTLQPTLSNQSKAAEAAGTTQDRSIMATIADDDDRLLVRIGYTPVLQRHFSRWSTVSYAISILGVLGSVPATFGAPMGAGGPATAVWAWFIGSIMAYCIASSVAELVSAYPTAGGMYYVTKHVVPPEHVAAWAWVIGWCNFLGQAAGVASLAYTISQMILATVVMHTLDNGVAAYEPEAYHTVLLAIFVLCMFGTICSFPTHWLHRIILWFAPINIIASICICIALLILTPNKQSPQWVFSTVTDGSGWGSQGFSFLLGFLSVAWTMTDYDGTTHMSEETHDAAIRGPVAIRAAILVSGVVGWMLTVTFCFCMSDYDEIMATPTGLPVAQIFFNAGGKTGGTIMWFFVMLVQFFTGCSAMLANARMAWAFARDAAFPFSDFWSKVNQYTHTPVNAVWLVVVFCTCLDLIGIGSTLTITAIFNITAPALDISYIAVIIAHRWYEGRVVFHPGPYSMGRWSKPVNAIAVTWVIFISVVLFFPTIKPVRPDNMNYAICVAAFIGLFSTVWWFGGARKSYIGPRTSDTIDMLPPEDPEDILSDYDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRZ9</accession>
    <name>A0A9W9CRZ9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000430</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRZ9"/>
    <dbReference type="OrthoDB" id="3353407at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021822">
        <property type="entry name" value="DUF3405"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36205">
        <property type="entry name" value="CHROMOSOME 19, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36205:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11885">
        <property type="entry name" value="DUF3405"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040799092" evidence="2">
        <location>
            <begin position="26"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="549"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="558"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="810" mass="94524" checksum="5AB0545055187022" modified="2023-11-08" version="1">MLVRSPRRFAILSFVAFAFIFAVLQQSPWAHEKVYERLPHFDLGSRPPDQSPIEDGSRTHPEDAWQHAVPVAKPSAIDVELVAGGAGVHPPIAPGQDVEETKSNPPWVTAPSRSKPLAPPTPRPSNMKEYMKKMLKWSRPTWDGHWPPFQEYTYKGYDPNRWEEFDMDDRVYTSGHHKLNGTIASDPIAYKPFPKYNTDSWKKSWKSEYVPCLGPRGIALNESTDDEVQVYRGVPADFPPVYAGGYDVVGLDDNVCFDRYSRYGPYGYGDDEFPEEVKHWKAPELIPDWPKVRWGELQDDCLERNKERYRPEARAPIVKVPSTVLPEPAAASSAFAIPVDTSRSNLEQREHQEQKYHHRTAVLIRAWTGYHYEENDITAIRAMISELSLQSGGEYQVFLYVHVKDRNAPIFRSQWEYNRILKENVPEELHDIAVLWSESIFPDWYPKVKDWQVYWQQFMCLQWFSLTHPEFDYIWNWETDARYTGHHYHFFEKISEFADKQPRKLLWERNKRYYLPSVHGDYQSFVEQTHQDVLNASAHSLIPPPAWGPRPWARADPPQQPLGPSPPTSFEDDDFNWGVNEPTDFITLLPMWDPRNTSWSYKDKIWNYLPGVRPIFTPKDGQADWFTHPDFVDIDRRVFINTVVRFSKRMLQAMHEENLVGRSMQAEMWPSTVALQHGLKAVYAPHPIFNDRHWPAAYSEAIFDTASTVNAITGEPLETRQGAWTEEADSPYNHDREYNFQGWSWYYASRFPRNLYRRWLNWPIMKQSWGEGEKVDGGPGFDGKLQEKRMCIPGMLLHPVKRMEKDKAMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSP3</accession>
    <name>A0A9W9CSP3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSP3"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF226">
        <property type="entry name" value="CYTOCHROME P450 MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4378189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="36220" checksum="46AD18EF41D19E00" modified="2023-11-08" version="1">MNLSARSPLRNLILWLKPRGLNQVMGACAPKYVKEFYSFVQQSVEQRYALFAKTQNIEKERPLNETAPREDMFHFLCAARDPSTNQPAFTKTDLLAEARLLLIAGTDTTSTALSASLFYINNDPRVYELLVSEIRSTFSSRDQITHGPLLNSCAYLRAVIEETMRMTPAGPSEPERLVRPGGMEVDGHFFPAGTLLGVPHWPYYHNETIFTDAWKFLPERWIASPENPISDIHALRRGYSPFIKGVNSCVGQNMALMILSITLARTLWALDCRIAPGTNLGEGRKELGWGRRNKKVYQVDDAFVSLRKGPVLQFRAREVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XX11</accession>
    <name>A0A9W8XX11_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_010820</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XX11"/>
    <dbReference type="OrthoDB" id="2123952at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF25">
        <property type="entry name" value="ABC-TRANSPORTER-REGULATING TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="641"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="62"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="143"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="747"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="166"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="747"/>
            <end position="766"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4361879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="905" mass="101111" checksum="0DD199AFEDE62ECC" modified="2023-11-08" version="1">MNTSPNGFYGAGPPMMNPPPPRIFGSGGFDSSQMPSGSMFAHDDMDDGNDGGDPKRRRIARACDMCRKKKIKCDGKMPSCTHCQNYKTECIFTQVEKKRQPPKGAKYIEGLENRLARMESLMRLSGVLPEDDDGSTDLATLERRLADRTGRADTPRTSLDERRTSALPQTTGEQSTTNGTPVQPALPSPRSGTASPEPEKRFEGEKEEDALAEMMCSLVTNNCGETRYIGSSSGFSIFSPKGIQWVNEKTGDNSFRDMISTAAVEDNKWIQWRPDVFQDIFKRRVYKQLPPKHEALSLLKDYFENFNCMFPLFHEPTFMHLVDKHYSDDPYEGSGWWASLNVALAFAHRLRVMSNLVPAEEDEKAWQYLKNAMSVMVELTMRNTDLLSVQALLGVGLFLLGTPNPQPTYFFVATAMRLAHSIGLHKKGANFSLNAAEVEQRRRVFWIAYMLDKDICLRSGRPPVQDDDDMNVELPSDTPPDNIGNIPLADGKGTMNLFRLMCTFSVIQSKVYKGLYSVKASKQTDGELLNTIGELDKELEAWKDDIPLEFRPEHDIKASHTPLILQVAVLHLGYYNCLTTIHRMSVHHGYWTSRLSNFAIQGLNARPLNPRVFMSAQLCVQAARASIHLLKYIPKGDLSCVWLVIYFPVTALVTLFANILQNPQDTRSRSDLKLMKLVVNFLNMLNDDQGSGSVKRMCTVCAEFERIATIVLEKADREHASRRKRKQGDSEQDAQIEATAAELLSTGRNAHSSPQQVTTPQTNPNASEGMIFNPDFQGFNEPFPFSPNFTHASLQANSQMGQFGSPSMTAQMLQQQQNLPLTTGGDVNGISSNINAMAMGQFDQTFNNVPQDLWKMPMTLEWDWADMTGYAGYEDGISMNGVLPDFSNSGHGTSEFNQSAMNGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXQ8</accession>
    <name>A0A9W8XXQ8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA topoisomerase</fullName>
            <ecNumber evidence="6">5.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TOP3</name>
        <name evidence="10" type="ORF">N0V83_010767</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXQ8"/>
    <dbReference type="OrthoDB" id="430051at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031422">
        <property type="term" value="C:RecQ family helicase-topoisomerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035825">
        <property type="term" value="P:homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03362">
        <property type="entry name" value="TOPRIM_TopoIA_TopoIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.290.10:FF:000001">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.140:FF:000003">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="entry name" value="Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="entry name" value="Topoisomerase I, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="entry name" value="Topoisomerase I, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023406">
        <property type="entry name" value="Topo_IA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034144">
        <property type="entry name" value="TOPRIM_TopoIII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390:SF21">
        <property type="entry name" value="DNA TOPOISOMERASE 3-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390">
        <property type="entry name" value="PROKARYOTIC DNA TOPOISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00396">
        <property type="entry name" value="TOPO_IA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="8">
        <location>
            <begin position="23"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="9">
        <location>
            <begin position="186"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="555"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4361826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="679" mass="75913" checksum="0EACAD6E11448FB4" modified="2023-11-08" version="1">MTANNPCLQSSPWTQRHVKVMARVLCVAEKPSIAKAVANHLGGQTRAESARGVAWVKNYKFDFRFQQWGQCSVTFTCVAGHIVAQDFHERFKKWHSCQPAALFEAPIESSIAQDKKAVASNIESQARHASILYIWTDCDREGEHIGTEIRDIALKANPNMQVWRARFSNIERAHVIQASQNPIRLDEAQAQAVSARIELDLRLGAAFTRMQTLALQNMIPQQGEERGKLISYGSCQFPTLGFVVDRYLRVRNFVPEPFWYINVTHTKDGIDVKFNWRRGHLFDRMAVVIIYERCLLSQVAKVITTAKKPTKKWKPLPLTTVELQKMGSRFLRMTSQDVMKVAEDLYTKGWISYPRTETDQFDRGMDLRTIVSRQTQDGRWGPFAQSLMNGGFSQPRSGRNNDKAHPPIHPVNYVASTHLNENERKVYGFVVRRFLACCSEDAVGEATDVEIEYGTEIFHVHGLTVIERNYLDVYPYDKWESSRQLPHYTVGETFEPTEANMLDGQTTAPGYLTEPELIALMDANGIGTDATMAEHIAKIKDREYVVARSSTGGIAAGGNGASRGRGRGRGGGGRGGRGGAAIAQNGGGTGGVQEFIPTTLGVALIEGYDNVGFETSLSKPFLRKEMEAQMKAICEGRTTRNDVVQQNLEQYRAVFNRTVQQINVLKSAVTKYVVNANHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYC4</accession>
    <name>A0A9W8XYC4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010017</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYC4"/>
    <dbReference type="OrthoDB" id="3509362at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08276">
        <property type="entry name" value="MDR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052711">
        <property type="entry name" value="Zinc_ADH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45033">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45033:SF3">
        <property type="entry name" value="DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G13270)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="1">
        <location>
            <begin position="15"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="356" mass="37663" checksum="638E29F8546B7936" modified="2023-11-08" version="1">MAASTTNLAWTIPSDASATNHLIKRSVPLPSPGPHQALIRLTAASLNYRDILIATRSPSYPGNHKPDLVPGADGAGIIYAVGPSSKWAGREGTEVLLHPNSWLSGDVRGLDLSKVLGGFDGDGTLQSWIVEDDDRIIPAPSHLTSLESASLVTAGVTAWSAIREGLDGRFDGGLEEWKGGRRLEGKTVLTMGTGGIAAALGATVIATSSSDEKLEHARALGAKHLVNYVSTPNWDDEVLRLTDGKGVDQVIEVGGARTLMRSINSTRPGGLISLIGILSAAEDIPKEFVPSVLFTGKIVKGCVAFSRDATAEFAKFVEEHRIKPVIAKEFDFDGAVDAFNALERQDAVGKIVVRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYV0</accession>
    <name>A0A9W8XYV0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Clr5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_009701</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYV0"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025676">
        <property type="entry name" value="Clr5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050745">
        <property type="entry name" value="Multifunctional_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24189">
        <property type="entry name" value="MYOTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14420">
        <property type="entry name" value="Clr5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Clr5" evidence="4">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="54"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="715"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="715"/>
            <end position="739"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4363408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="784" mass="87105" checksum="32BC9DEADFEB42FD" modified="2023-11-08" version="1">MTKDWDAVQEEIKELSFNQKKPLEEVKELMERKHNFRASTRAYRMKLKDWGFMRHKPRRTTKDRNKTSDTNTTAREDDEQRERDSSGTIEPMSATACEKQGGWQVVANADVEAEPTFMGLLSRSRDLQPSFDPLAMARTRASNIVLDMLGAMLDRDSQKLESLIAEDLNHINDPIGMPFDGPNSRFSGHPALSQMVILQHPDQTLLDIACGMPCGPVIWILLAHGAKSSKHPLGTDLALHNAIKNGRPYTVQALLVPGRSDVNGLAESTWRPLRQAVFCNVPDVVRILLSRGANVDDSGPSPFSMGSYTALQLALDYRVTKYMDPPAKERCHQTLKLLLEAGADVHRAPAEGSTQTSFEVFIRPWQGVPYWAAGLTETELDCFRLLVSKGADVQTQLDGYPCQSPWSRTFEHQVLWHSTPSVARLLIDSFVVNPQNCSGLLHDLLGSCPDAKRHPADTLRDIEVLLGKGMNPNFTDINGLTPLEKCINLCPAVDLVARLRMLLENGADPELDGYSGVPLYVIAARTFEAPLLVEVMQALVSKIRGRYTRLVDGISHTWPEGIFPISETQSYEQVMSCTRNTGDFVLNMRQMVPEDVQSSFQRGYFSVVSKNYLDTMTKVAKRKMLDHNEKNEIVWIISMRKHADYPEYKFDQELIVALLDPQPFPSMFLEPVAETTTTSATLLDSTSSAISSPTSSLTITSTAASPPVHVPFQFNPNSSSTTPNLTIPTSTSPSQNEVPSSAECDFVPMITQIRWKDPCATPRPDDLRRTVASVLEAGKASCGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ23</accession>
    <name>A0A9W8XZ23_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_010027</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ23"/>
    <dbReference type="OrthoDB" id="5378633at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF160">
        <property type="entry name" value="SAT4 FAMILY MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040967576" description="Rhodopsin domain-containing protein" evidence="7">
        <location>
            <begin position="21"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="28"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4362910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="161" mass="18191" checksum="6824FBEB2F5411F0" modified="2023-11-08" version="1">MRLLKATIAPLVMLIAFTSAADTSNAALLALKEYFFLGEIAYLEGMGILKIAILWFFLSIFPDKQFRKSVYIVMGLCVAYCITFLFATVFQCTPVTYTWTQWDGLHPGKCNDIHVQGWISAIINIILDLVVMALPLRQLSRLNMNWKKKLGVMSMFLVGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZS9</accession>
    <name>A0A9W8XZS9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Glycosylphosphatidylinositol (GPI) anchor assembly protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">GPI11</name>
        <name evidence="10" type="ORF">N0V83_010533</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZS9"/>
    <dbReference type="OrthoDB" id="17366at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009580">
        <property type="entry name" value="GPI_biosynthesis_protein_Pig-F"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06699">
        <property type="entry name" value="PIG-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4362440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="248" mass="26226" checksum="CED543E0FB60B1F7" modified="2023-11-08" version="1">MASTTVVNANATSKTPPSSAAPIEPLHNDTARLYTHVHPILVLSLYAYKFTSLVADPVPTLLSTLAPLAVLQIAYVAACLPPTGGTPAIKKQKPGEKRGRVPGKLESGLNAKLVPAFLSLLLTTLAATPLLTATLVLFGAPVTTHHLHTLLCGAHIALLSTLPLVYVHGVTGETWRQAVALLLPIDEVYGALLGTVLGAWLGAVPIPLDWDREWQKWPVTIVTGAYIGYATGKLLGGTLLKGKKIMFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZU1</accession>
    <name>A0A9W8XZU1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Auxiliary Activity family 9 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_009601</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZU1"/>
    <dbReference type="OrthoDB" id="4849160at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd21175">
        <property type="entry name" value="LPMO_AA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049892">
        <property type="entry name" value="AA9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005103">
        <property type="entry name" value="AA9_LPMO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33353:SF34">
        <property type="entry name" value="ENDO-BETA-1,4-GLUCANASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33353">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12560)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03443">
        <property type="entry name" value="AA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040884432" description="Auxiliary Activity family 9 catalytic domain-containing protein" evidence="6">
        <location>
            <begin position="21"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Auxiliary Activity family 9 catalytic" evidence="7">
        <location>
            <begin position="21"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="248"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="299"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4364146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="350" mass="36097" checksum="69BDEB04B249B466" modified="2023-11-08" version="1">MYKTVASLGAIAALAATATAHGTTQGVTIDGQYIGGFKLDYYYAKQNNAPIPDHIGWYAEDLDNGFVEPNSYGTADIICHKNASPDGSSDTMAKVAAGGTVEFHWTVWPDSHMGPVITYVAPYTGDIASVAKEDLKWTKIDAAGYENGEWAAIKMIGQNNTWPVTVPAALKAGKYVFRHEIIALHGAGSANGAQNYPQCLNIEVTGDGTDAPEGVVGTSLYTVDEVIFNPYAATINYTVPGPALWTGGSSSGTPTTPTTPTTSAKASSAPTATASSAPSSAPAASASAVVTPVEEPTATASAVASATPSSGAGGSDELPTEFTIETFISWLEGKAAGSSSKARRHARSFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0F9</accession>
    <name>A0A9W8Y0F9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_009362</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0F9"/>
    <dbReference type="OrthoDB" id="1306014at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20908">
        <property type="entry name" value="SUF4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000354">
        <property type="entry name" value="C2H2 finger domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003656">
        <property type="entry name" value="Znf_BED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23215:SF0">
        <property type="entry name" value="BUB3-INTERACTING AND GLEBS MOTIF-CONTAINING PROTEIN ZNF207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23215">
        <property type="entry name" value="ZINC FINGER PROTEIN 207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50808">
        <property type="entry name" value="ZF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BED-type" evidence="9">
        <location>
            <begin position="13"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="42"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="201"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="238"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="287"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4363910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="328" mass="36333" checksum="6638BF6AFF208E0D" modified="2023-11-08" version="1">MTKKRKRYPDLHQKLERPWCYYCERDFDDLKILISHQKAKHFKCDRCGRRLNTAGGLSVHMNQVHKENLTHVENANPGRQGLDIEIFGMEGVPAEIIDQHNQQVTQAHFAEEQERARITGNPIRGLYPNGQAPPVKRERVDEKIEEIENRADQFRTDVQNGVLPQSKTEAVTNLVCVVLAMEMDTASRPITLRSTTICLPTRRPSPGYPSPFPPSANGAAMPPRPGSIPGQPIGGALPPRPGFGAPPPLAAGYAHAQNGADPNAISDSIDHLIGEVTKEAAAAPPAAEKKEEKKSKKDKNMKLIFFDDTASPEEKMTSLPRFAEFVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1C1</accession>
    <name>A0A9W8Y1C1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DASH complex subunit DAD2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Outer kinetochore protein DAD2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">DAD2</name>
        <name evidence="19" type="ORF">N0V83_008774</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DAD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1C1"/>
    <dbReference type="OrthoDB" id="3230169at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013963">
        <property type="entry name" value="DASH_Dad2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28036">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28036:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08654">
        <property type="entry name" value="DASH_Dad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00030568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ4365156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="138" mass="14817" checksum="4B273714ECF76C5C" modified="2023-11-08" version="1">MSNYARPLPSHLRNQSLGGSQQQSPVLLARIAEKKAELANLKDLQALSGGLADQMQMLADKLQTLSDGTEAVAAVLSNWHNVLRAIHMASTKLPRPKEEENVEKEDEAGPPLPQTLVRIPTQHAPAVIEQANAGASAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1Q5</accession>
    <name>A0A9W8Y1Q5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="9">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SHM2</name>
        <name evidence="11" type="ORF">N0V83_008299</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the SHMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1Q5"/>
    <dbReference type="OrthoDB" id="10265628at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000097">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF28">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="10">
        <location>
            <begin position="19"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4365679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="471" mass="52149" checksum="4007B7D493C68D24" modified="2023-11-08" version="1">MSTYALPKSHQDLMEKSLVETDKDVAEIMEKEIQRQRESILLIASENVTSRAVFDALGSPMSNKYSEGYPGARYYGGNEHIDSIELLCQKRALETFGLDSEKWGVNVQCLSGSPANLQAYQAIMRPHDRLMGLDLPHGGHLSHGYQTPQRKISAVSTYFETFPYRVNLDTGLIDYDQLEQNALMYRPKVLVAGTSAYCREIDYARMREIADKVGCYLLMDMAHISGLIAAGVNKSPFPYCDIVTTTTHKSLRGPRGAMIFFRKGVRKTDAKTGKETLYDLEGPINFSVFPGHQGGPHNHTITALAVALKQAQTEDFKLYQQQVIKNAKALEVAFKELGYTLVTGGTDNHMVLLDLKPFALDGARVEAVLEQVNIACNKNTTPGDKSALTPMGIRIGAPAMTSRGLGEEDFKKIATYIDTCIKLCKKVQAELPKENNKLKDFKAKVASGQVDEINSLKKEIAAWAVTFPLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1V8</accession>
    <name>A0A9W8Y1V8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008852</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1V8"/>
    <dbReference type="OrthoDB" id="416217at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4365234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="247" mass="27992" checksum="4468CC9B307C564D" modified="2023-11-08" version="1">MAAALALAFQSSHLQDGMAEFLTMIRGCNLIAGDAAAMTSDSAFHAFREDGHLQTMRSRIGTSQLNCVNREDLDLAAMSLREIEQLNLSDWERTYWDILVRTVDNAYERPVESYTTFVSLYNIPSRWTHDEFQAFIDPNNSVAQILLAHFIAIQAILTPILYLERVGFQGVDAPTAILSWIDGVYRNVPPHLRHYVEWPRQVSRYPFMRFLGQKQVDYFDEDLLLAVYNSGDVGIDGEGGSPDQHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y235</accession>
    <name>A0A9W8Y235_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008465</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y235"/>
    <dbReference type="OrthoDB" id="66095at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4365844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="259" mass="28855" checksum="F74882082091A714" modified="2023-11-08" version="1">MSFLPSIMNFMPSLDWLRGPSQPSGPFTPTYEDVCRARALLKALQLPTELVLDILEHARYWPINEFNTVPNRHIVAASRGGRPSAATLCLDAGIFNNPTVNAIRAGGERPRIKSVEFDIVSHDQGHTSEGTEGTFTTSSWLEVSILRNETNSIYRLPGPRLVNTWISSPLDFHTNMVGRGWFLVSRPKSAQQGPQDGEGDLSWYLQGNRVTAGREKYRVIWTDDGSEGNEGAGRGDDFVQELTDGDRLIIWARAKVRTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y244</accession>
    <name>A0A9W8Y244_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008157</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y244"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051948">
        <property type="entry name" value="Hsp70_co-chaperone_J-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="9"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="80"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="228" mass="25818" checksum="5E297138EC0418C5" modified="2023-11-08" version="1">MATTNPKPDYYAILEVRPGATHDEIKASYRRLARVHHPDKNDGARSSTIKTQQEKVSHLDSIYVEVQQLNGAWEVLGDSGRRAAYDRSRPAQTTARPPPPPPQQQQQAPPASQPAQEEEESEESRAERASRERREWLTWEKAQEEAIAQCQAGMVASEAEIAALAAANDPAIRLKRVLLGCQKSRLDWLLKTLAQRRVQEDVRLRVEREEEVSRERAARERSQRGAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y281</accession>
    <name>A0A9W8Y281_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Class E vacuolar protein-sorting machinery protein HSE1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Class E vacuolar protein-sorting machinery protein hse1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">N0V83_009273</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the ESCRT-0 complex composed of HSE1 and VPS27.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the STAM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y281"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11883">
        <property type="entry name" value="SH3_Sdc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053235">
        <property type="entry name" value="Ser_Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24361">
        <property type="entry name" value="MITOGEN-ACTIVATED KINASE KINASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24361:SF433">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 17" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SH3" evidence="19">
        <location>
            <begin position="169"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="325"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="229"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ4364676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="691" mass="78426" checksum="082DD25CEBB31AEE" modified="2023-11-08" version="1">MDRLRQEEANEKRRQEERVRNIKHNARIQAAQEEQKRNDKRGFSFSRLFRSPKPNPKAALVKPHAEEDEKVAEAARKAAEDSKFARLENLLNSQQEARIDRDKAKKKGDEDKLAILEKLPESQGKQLAGYLGSLERRPTPEPGRPAKDYLSQVYPQHTPLSVPTQGQQQGGVYVTALYDYEADEDTGLSFHRGDIIQVISVLESGWWDGVLNGIRGWFPRNFCTSEPGHLSDEDSLSDSSQNTSGDEGSHSGKGATGVSVMNKDGTVTYQPLLDGMTVAHGVLAWRPNQWTKVLDESKFLPERDPRTYGWEMEHRMFGGEDEEPGVVCNVLGHGSLGIVDEVRRRDTTLPTFVRKRVQLPARKAQAKAILNIIQGECENLKSLVHQHIVTLLGTYEEMRHKNRHLYFLLMSPVGDNDLKNFLDIAGEHLEQDRTEPRVDLWRSWIYNWFTCLASALAYMHGKGIRHQDIKPSNIIHKGERIYFTDFSLSCAFDLGNTTSTDNPSRSSPMYAAPEIVDKYNGEIKRHGRASDIFALGCVFCDMLTILTGRSVSSFHEMLASDKNTANEDTSQIRGPLYYSRKLSSIHTWFASSHIFAEHISEMLGSERTSRPSAFAVVQGLVRDHIFDDDCMCWIDALWYKNKEGVLVRRNHGTIPLLFGDGTTEVMDCPDWEMDTIEQHGIKITPWNERKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2G3</accession>
    <name>A0A9W8Y2G3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008502</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2G3"/>
    <dbReference type="OrthoDB" id="5342507at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="26353" checksum="F0660CC25D533CD6" modified="2023-11-08" version="1">MARHHQQPIDGPVDYPVVDKREQTGLLTRFLPQSLKNGAFYRTLLRLTRLLQFLSSVISLGIFSQRMYKVYRLVNSIKTRRGVNGAYGAVEGILAAAVLYTLITTILGCIKKSANPGGRGLRWAWVLLDLLFVGAFIAVSVLTRSKGGPAGPRHCYNPSRLSSDSSNVTGQTANAKDDSCNLPWGTFILAIISTILHAITAAFHDIKDHHRRNKLAHEKAAHEQHASGTVPPVRDDSRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2L8</accession>
    <name>A0A9W8Y2L8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2L8"/>
    <dbReference type="OrthoDB" id="3787285at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4364433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="279" mass="31363" checksum="3FCCFB9F77FF916A" modified="2023-11-08" version="1">MDQSALIDFGAGGEIEVERRALSNWFPLLAYQFGDINGSSMVNKLASRLHRYNISKAIIRDTLQTLFENLSIVTLPVNGETRMRVTATLKQLLKDDLSRHRFNASGFYLSRRSFVILAYLADMYRSELLAGALWEFWLACGHGIVQGDQMYLDTDWLPAVGKLQRIGTDGMLHEPARGGFGALATIPYGGWNRGRMMGALPWHGRAISAPATRRHYSPDMSMVLANLPGPAWTPPVMSPVGFPKTRYFDDITALHYQQQGMNRKLEDIDQKLDLLIEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2N4</accession>
    <name>A0A9W8Y2N4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein-L-isoaspartate O-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.77</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_008243</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[protein]-L-isoaspartate + S-adenosyl-L-methionine = [protein]-L-isoaspartate alpha-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:12705"/>
            <dbReference type="Rhea" id="RHEA-COMP:12143"/>
            <dbReference type="Rhea" id="RHEA-COMP:12144"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90596"/>
            <dbReference type="ChEBI" id="CHEBI:90598"/>
            <dbReference type="EC" id="2.1.1.77"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.77" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2N4"/>
    <dbReference type="OrthoDB" id="73890at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000027">
        <property type="entry name" value="Protein-L-isoaspartate O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00080">
        <property type="entry name" value="pimt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF0">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE(D-ASPARTATE) O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01279">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4365623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="222" mass="23802" checksum="DDB1EB1C6165156F" modified="2023-11-08" version="1">MAWRSSGASNEALIANLAKNGLIESDRVKEAMLKVDRAHYAPSSAYEDSPQPIGHRATISAPHMHASACESLLPNLSPGSRVLDIGSGSGYLTAVLANLVGPSGTVIGIDHIQPLVDMATTNMRKSEEGRRMLESGQVKFIAGDGRKGWKEGAPYDAIHVGAAAAEHHQELTEQLKAPGRLFVPVAEGWVQHIYVVDKKEDGSVEKKKLYGVQYVPLTDAPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y331</accession>
    <name>A0A9W8Y331_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF3431 domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007344</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y331"/>
    <dbReference type="OrthoDB" id="426718at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021838">
        <property type="entry name" value="DUF3431"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37490:SF3">
        <property type="entry name" value="DUF3431 DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37490">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11913">
        <property type="entry name" value="DUF3431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="35124" checksum="2990517011A83B77" modified="2023-11-08" version="1">MCEAMIAPLRRKLVALAIVTTLFFLAVFRFTYQRLSVDDIRAFVGASPFEGHIASKLLGSSPTTTRDDVQPTPGTNGSSKMTSSATFTPHPGTQHKILVVARTKEENTDWVYERLHDWPKAVYTVDDPSAALHTGKNKGHEASVYLTYIVGNYDSLPDVVVFIHSHQKHKHGTKRDRIFEGIDYDNLESIKALNLDFVKRNGFTNLRCLNNPGCPAEIQPFRPDAERDPLRPQENAMAGAWTDLFQDDNVPRVLAAPCCAQFAVSGLQIRQRPKAEYERFLIWVFNTPLDDFTSGRVFEYLWHVIFGKDPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y334</accession>
    <name>A0A9W8Y334_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y334"/>
    <dbReference type="OrthoDB" id="5404940at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="479"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="662"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="508"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="524"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="550"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="568"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="681" mass="76389" checksum="34F3D1B669B28C0A" modified="2023-11-08" version="1">MDRWQPYLYEPTRDSMDVKSTFNPKAVTMASRTSVPPRKKQDGPYINFNKHPDSFLILPYGNTDAKPMHAKTKVFINVTRWIQLAFRVCAMLGAVGVLLCGIFIKGAADTEGYIMRIPPGVDIVVCLYAVYHLVRNAKSRPPASSASYHFFALVTDAGFIPFYVFTVLMSRRNSDMEAGTVGRWRSMFPTDGETNTVLMTTWLTATVVAGLHLVSLFLDLYLVIVFRKISKLPPDMNPLEDNLTRRKSKHKHKNSSISAITPLTGDKEKRFSAQSSGSGDRNSQADPLLRDIPSPNKSQVAFLHTRMNSETTYSPHTPNSARQSKERFSMYSQPPSARQSRKDLNHRDDLHRREDNADTETLAQRKSFLAQQANIKRHSRNDSYVTLSSAKQEFYTPPSTAQTVKQEAAGDLALQRNSRETLQNDNWFVHADEDDEKENEEHYHPEPPRHSVFVSKQGYNTLSPYDDVSDLEDEIEKPMMPQPLRMNPPTPPPVMNFDEKKSTPPPTTLKRTQTTTSISTEATFNRSHSRSGTPKSRYYGDLRAATQGIKNGNSPSNSPKTSPTKGSFRNQPNHLPSATKQYAVNTPPAAKNDFVTNSPFTLNKKSYTSVRRTGEANYTPVKGQSPRVVSRSGVDYMNPYEFDDSDLGTPGRRRDVSGKIAEEGRGGMWGGMTQRKVSGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3L3</accession>
    <name>A0A9W8Y3L3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">HRD1</name>
        <name evidence="19" type="ORF">N0V83_008287</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HRD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3L3"/>
    <dbReference type="OrthoDB" id="7759664at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16479">
        <property type="entry name" value="RING-H2_synoviolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057992">
        <property type="entry name" value="TPR_SYVN1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058051">
        <property type="entry name" value="Znf_RING_synoviolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763:SF184">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SYNOVIOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25563">
        <property type="entry name" value="TPR_SYVN1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="322"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="384"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="634"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="393"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="661"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="687"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="703"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ4365667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="744" mass="82271" checksum="AF4B8F00C0F76B97" modified="2023-11-08" version="1">MANVVLLRAYYERPNFYSAAVYISQSTGSLMFLVNLMLIVAASFGYGLQRLFYGPLRPIETEQLYDKAWFAVSETLLAMTIFRDDIGMWFFAMFLCLLAGKVWQWIGEGRVEFLEQQPPANPKLFHARLISSLLLSVGFDIFMMQYCIDSILSDARPGVMVMFGFEYVLLAIASISTALRYTLSLVELYITRRQENAQEEAQRAAREQARLRAAADGVEAPTGLEDEEDEDGDVPGWEEKGRWVFYLDLATDFIKSVVYLGFFMILMTFYGIPIHIMRDLFMTIRSLIKRINDFVQYRNATRDMNTRYPDATAEELERENTCIVCREEMRPWTHLGAEGAQAGRRMDERQRPKKLPCGHILHFGCLRSWLERQQVCPTCRRPVLSQPTSQDSQQNNQANQGQQNPQNQPGQGVLQGFFRGNPQRGAAGQQGQGQQAFPQGAPPNPPAGNMRVFNFGPFRIALGNLRIPQGQQDNAAVRNAMDQLRQQAIANHAGPNPAQQHQNQPLVQPFAPNITSNFVTGPVPGAVALHPADIQSDILRIQQNILESLRQLNAQHEQLEVVHRLLGELNRLQQASGITAATGQELPSIAPLNPPSMNSFTPQAYFSRGPVLSQGDAGVPDGLTLPEGWTLRPMALAPRPGQESATSPPHQAPQPTVGLPPSTSTEENSRSQPDSSSGAPTGATAGSSPKPAESSSLESSWSFGNVQGRNDMESSTSAVAGSSAEGQNMTRRTASVEDAEDNEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y451</accession>
    <name>A0A9W8Y451_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008559</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y451"/>
    <dbReference type="OrthoDB" id="3557318at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="10"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="371" mass="44251" checksum="B95BABFF33BBB206" modified="2023-11-08" version="1">MYPPSWLHEKGELEDKVRKCNHRCDELKRKVKELTESFEKKKHELTETIEKKKHELHETKETLSKVKYEKEELHQKVKELRHELEEEHCRWEDAEDRCGKWKLKWEHSDREVTSLTEEVRIIEIEKTELQETITKKTEEYRRLVIEKKRLEKDHHGRCEESEQRHRDIIVLQESIRRLETTLMEKTETIHTLTERIERVERDRDDLRVKLSDAEIELSTSQSTLISLNLDIETITKERESLREKYHECQTRYEEVCESVTEYHEGNSSFEYEISSLRGMLREVREEREKAIGMRLSADRERDEAVSRYEAKCREMDKLEESFALRSVKERSSGSRTVSRRFFHGSSSVGGVAEGGSGEDDSGSVAWRCYLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4B9</accession>
    <name>A0A9W8Y4B9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Clavaminate synthase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008052</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4B9"/>
    <dbReference type="OrthoDB" id="438224at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000033">
        <property type="entry name" value="Clavaminate synthase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48420">
        <property type="entry name" value="NON-HAEM DIOXYGENASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48420:SF1">
        <property type="entry name" value="NON-HAEM DIOXYGENASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4366416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="358" mass="39327" checksum="3263316D8382DABB" modified="2023-11-08" version="1">MAAANSAVNQNAVAVGLEDLKSNNVDFALLEEAFGPSSLGIIVVKDLPSKFHELRHKLLSYSSALGNLSPEELEKLESPASKWLVGWSCGKETLKDGRYDTLKGSYYVNCASSFEEQQKAVAQKYPSFPEYTAPNVWPSEELLPGFEETFRELCTLIIDIAALVARACDKYAEANIEGYQKGYLEHVVKTSVFTKARLLHYFPSPESSSNSSRETSDDEDDWCATHLDHGCLTGLTSAMFVDEAANVPQTGSAFAPLKELERSPDPKAGLYIHSRTGAVTKVSIPRDCLAFQTGEALEIITRGKFKAVPHFVRGAGPGIGGKVARNTLAVFTQPNLWEKLDERRDFAAFAKEIVEKNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4E5</accession>
    <name>A0A9W8Y4E5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA polymerase II holoenzyme cyclin-like subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SSN8</name>
        <name evidence="5" type="ORF">N0V83_007161</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin C subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4E5"/>
    <dbReference type="OrthoDB" id="10266018at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20513">
        <property type="entry name" value="CYCLIN_CCNC_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028758">
        <property type="entry name" value="Cyclin, C/H/G types"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0195">Cyclin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="4">
        <location>
            <begin position="53"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="4">
        <location>
            <begin position="154"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4367577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="293" mass="33711" checksum="B9CF7390EC7F065E" modified="2023-11-08" version="1">MASSYWESTQRKFWTFTKQQLALERKKMEESERNLVNMYPLPDRRHLSIYFYHQLSKMARPLGIRQQALATAQVYVRRFYAKIEIRRTNPALVLATALYLACKMEECPQHIRMVLAEARHCWDTSFNDISKIGECEFTLISEMNSQLIIHHPYRSLAELQTQFQLTQEENALAWSIINDHYLTDLPLLHAPHVIAITAMFLAVVLKPTQGGLQVNAAGMTNALQTLGNARNAGGTQNRVQKLVDWLSESNVDIEAVVECTQELISLYEVWESYTDKTCKDQIAKFVKARGLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4S0</accession>
    <name>A0A9W8Y4S0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C-CAP/cofactor C-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_008261</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBCC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4S0"/>
    <dbReference type="OrthoDB" id="194775at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1250:FF:000002">
        <property type="entry name" value="Tubulin-specific chaperone c, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1250">
        <property type="entry name" value="Tubulin Binding Cofactor C, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017901">
        <property type="entry name" value="C-CAP_CF_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027684">
        <property type="entry name" value="TBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031925">
        <property type="entry name" value="TBCC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038397">
        <property type="entry name" value="TBCC_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012945">
        <property type="entry name" value="Tubulin-bd_cofactor_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15139">
        <property type="entry name" value="TUBULIN FOLDING COFACTOR C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15139:SF0">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07986">
        <property type="entry name" value="TBCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16752">
        <property type="entry name" value="TBCC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51329">
        <property type="entry name" value="C_CAP_COFACTOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-CAP/cofactor C-like" evidence="7">
        <location>
            <begin position="174"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="116"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="121"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4365641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="372" mass="40942" checksum="1E48FA97616A8A0F" modified="2023-11-08" version="1">MATSPASTETGLKERFFRYFQHEVTALQEQMERLNQMSISGGERTDAVDHCLAGINRLSHEVKDASNYIPAYDQRTYSEAIKALSEKLQNTRNAFNPPKKFQFKSRKTVASAISVNNAPVPTSDTANTNSSSAPTPLANPPPKEEKQQQQQQQEAPADNVEEDGIDGIKADNMGIRRPSFSHATKITISNHSSLHIIVPPSASHATSSGTLSHLRGCVVDLSAATTTEAPSAAFAALYLKHIKDSLIICGQIAGAIHITDVSNSIIVTSCRQFRMHDSKNVEVYLHSASRPIFEDCEGLRFAPLPDVYMTAETRQAANQWDQIDDFKWLKAEPSPHFTLLPEAQRVQGEVWTEKVGSGRTSLDDTLKAVRVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y532</accession>
    <name>A0A9W8Y532_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ubiquitin-conjugating enzyme E2 1</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">E2 ubiquitin-conjugating enzyme 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">E2 ubiquitin-conjugating enzyme 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Ubiquitin carrier protein UBC2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Ubiquitin-conjugating enzyme E2 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Ubiquitin-protein ligase UBC2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ubc1</name>
        <name evidence="15" type="ORF">N0V83_007657</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="13">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y532"/>
    <dbReference type="OrthoDB" id="9993688at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23800">
        <property type="entry name" value="UBCc_UBE2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000037">
        <property type="entry name" value="ubiquitin-conjugating enzyme E2 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015368">
        <property type="entry name" value="UBA_C_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09288">
        <property type="entry name" value="UBA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="14">
        <location>
            <begin position="4"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="12">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4367127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="245" mass="27241" checksum="734EB66BBD777A35" modified="2023-11-08" version="1">MASNRARRIAKEIADIQNDQLSKIVVEPFKDDDLTHLRGQFFGPPDTPYEGATYLVDIKIPNEYPFRPPIMKFETKIWHPNVSSQTGAICLDTLSSQWSPVLTIKSALISLQSLLSTPEPKDPQDAEVAGMLIRNPAEFEHKAREWAVKFAGAPKKEIAEGSGGATAESIIKKKQQAKQNEEKVKLAAYHGYNKDLVDRFVAMGFDVEAVVASFEYVGIDKMGGEDYELEEAYMGDITARLFGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5A1</accession>
    <name>A0A9W8Y5A1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RPL6_2</name>
        <name evidence="6" type="ORF">N0V83_006670</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5A1"/>
    <dbReference type="OrthoDB" id="2436667at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13156">
        <property type="entry name" value="KOW_RPL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000014">
        <property type="entry name" value="60S ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000915">
        <property type="entry name" value="60S_ribosomal_eL6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049633">
        <property type="entry name" value="Ribosomal_eL6_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041997">
        <property type="entry name" value="Ribosomal_eL6_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10715">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10715:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01159">
        <property type="entry name" value="Ribosomal_L6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01170">
        <property type="entry name" value="RIBOSOMAL_L6E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4368314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="205" mass="22853" checksum="34F3A5E0FBA1F80D" modified="2023-11-08" version="1">MAATKDDNKPTTQKFSKGERSIPHHSQKASKYYPATDVAVPKKARKTVRDAKPRQSLQPGTVVILLAGRFRGKRVVVLKHLPQGVLLVTGPFKVNGVPLRRVNARYVIATSTKVDLKGVDEKVLEKVSEDGYFTKDKATRKPGEDSFFKQGEKPEKKETSKDRVADQKAVDKALLATIKKEAHLIDYLGASFSLRSSDRPHQMVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5I6</accession>
    <name>A0A9W8Y5I6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tachykinin family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5I6"/>
    <dbReference type="OrthoDB" id="4159781at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37540">
        <property type="entry name" value="TRANSCRIPTION FACTOR (ACR-2), PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37540:SF5">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="661" mass="73061" checksum="E11803D8141112FC" modified="2023-11-08" version="1">MEKAGNGGLEFITGNTPNELRSKKNMTTVRKKAMDHFLKDGKKGNGKSRDSADRSTRLNSEASDKSRSSIGSHEAGNDGQLVARPTSQQRTTRRKQKSRTTSSTNHGSSPDSQVARFQGPGGSVLPPAPIVLPMRTNVQLPYDNHVPPPLGSIGKSLDPFRTMFQATHPHVSVEELKFHCSRYFGTRGLGKYWIPTCLNYPHTFLSTLCLASAHHDIVHELPVESLATAALRQEIIHLVGANLLNPDKSVADHNIVAVTQLIIGEVIGREESGLLYHEAGIETMIKQRGGLNQLGMEGRLASAISWVTLASAILREEKPRTMYADYCTARSTKNYPPTATIPESPIYCPRGKFVTLERSKKCKQQTLELLSDIRMMIDVFLHETKQSRRNSQTLLNLYKKITNDYVPITELQKINILTPADWKYEAIRVAAEIQATAIMRRIPLSEALTHAAVPRKLATLYTSSTASRSSDSLVSPFDVRHDTPMTEYSTSPYSTYSTSPAMQPSSFPFATERPSFSSTHRSSDAPRPSFSSVNSISSEWLSFAPPRPLPTTPSRPTALLRSLKTALENSDLSDCWSDMAGVLLWIGLVVGAASRHSESKILKKYFSATAMRAGIMLCFEHPEAIHATMLRMGEVVDALSGENKGEIVRRESEGVGKRRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5W6</accession>
    <name>A0A9W8Y5W6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome maturation factor UMP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_007230</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the POMP/UMP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5W6"/>
    <dbReference type="OrthoDB" id="15001at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008012">
        <property type="entry name" value="Ump1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12828:SF3">
        <property type="entry name" value="PROTEASOME MATURATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12828">
        <property type="entry name" value="PROTEASOME MATURATION PROTEIN UMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05348">
        <property type="entry name" value="UMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00043974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4367645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="17363" checksum="6F0F8E13B988F5FF" modified="2023-11-08" version="1">MAKIQSLRFVPTKAHPTQTALETSAPSAPGVHDTLRSRLAQTSTAPSVSTSSSTAPIALQSAHPLEARLTQWRDTQDRLKMEMLRRQFGIAEPVRRGMELKIATAGEWRPAALGGSSGVHKDILEGRDCEIGWEDVFTGELLSSSGTVIPDSKQMLTIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y679</accession>
    <name>A0A9W8Y679_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006027</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y679"/>
    <dbReference type="OrthoDB" id="1731983at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08948">
        <property type="entry name" value="5beta-POR_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01833">
        <property type="entry name" value="XynB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055222">
        <property type="entry name" value="PRISE-like_Rossmann-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32487">
        <property type="entry name" value="3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32487:SF8">
        <property type="entry name" value="NAD-DEPENDENT EPIMERASE_DEHYDRATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22917">
        <property type="entry name" value="PRISE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRISE-like Rossmann-fold" evidence="2">
        <location>
            <begin position="5"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="SGNH hydrolase-type esterase" evidence="1">
        <location>
            <begin position="421"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="579" mass="64207" checksum="EBDD3D6B3A9ACEE8" modified="2023-11-08" version="1">MGNHALIYGASGISGWAIVNAILNGYPSKDAFSKVTAMVNRPLTREMALWPEDPRLQIISGVDLVKGTQEELEQQIKEKVKDVESVTQVYFYSYKQIDDPDQECKVNEAMLERAVTAIDHLSSKLSYVLLPTGTKIYGCQMLDKFPFGNDLPLKETLPPIPEPYLSQLFYYNQIDCLKRISKGKNWSWCEVRPDNIIGFVPNNNAYCLAQTLALYLSVYRAVEGEGAKCPFPGTEKSWVNKYNESPQDMVAHFSIHASLHPEQTASQSFNVGGQEDSWSGKWPVICDYFGLKGSGPEENSPQPGAYIAAHRKNWDELEAKHNLKKGSVDSDISHPGFQYFIMTMFDFDRQMSMEASHKVGYTEEIRTPETWKTAFDRMRKAKPAHQQDGTNGYRLRLLSDLLAAKFDVVDFVGTQHSGSMADSDNEGHPGFTISQIKNVMGPGLELKPNIVLLHAGTNDLNRAETAEESWADAPKRLGSVIDDVLKVNPDAVVIVAKIIQAANVQTASNLKTFNKVIPSIVEERVQKGFKVTVVDQSVVGADELVDGLHPTDAGYAHMGDVWFRGVNAASAQGLITPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6C9</accession>
    <name>A0A9W8Y6C9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rho GDP-dissociation inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RDI1</name>
        <name evidence="6" type="ORF">N0V83_006644</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Rho GDI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6C9"/>
    <dbReference type="OrthoDB" id="1683373at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005094">
        <property type="term" value="F:Rho GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.30:FF:000001">
        <property type="entry name" value="Rho GDP-dissociation inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.30">
        <property type="entry name" value="Coagulation Factor XIII, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000406">
        <property type="entry name" value="Rho_GDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024792">
        <property type="entry name" value="RhoGDI_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10980:SF3">
        <property type="entry name" value="LD16419P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10980">
        <property type="entry name" value="RHO GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02115">
        <property type="entry name" value="Rho_GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00071407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4368288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="197" mass="22582" checksum="BD1D9C6BA9EE6A04" modified="2023-11-08" version="1">MADQHDDLHAEQTEGFKVGEKKTIDEYQKLDQNDESLQKWKASLGLGQGNDISDKDDPRKCIILSLGLEVEGRPDIIIDLRDPGALEALESKPFIIKEGAQFRMKAQFKVQHEILAGLKYLQKVTRMGMSDKLQEMMGSYGPNTEEKPFYEKKFEPETAPSGITGRGHYKALSKFVDDDKQVHLQFMWSFDIKKDWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6K7</accession>
    <name>A0A9W8Y6K7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_006091</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6K7"/>
    <dbReference type="OrthoDB" id="684045at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18497">
        <property type="entry name" value="BACK_ABTB1_BPOZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000248">
        <property type="entry name" value="Ankyrin repeat and BTB/POZ domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044515">
        <property type="entry name" value="ABTB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46231">
        <property type="entry name" value="ANKYRIN REPEAT AND BTB/POZ DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46231:SF1">
        <property type="entry name" value="ANKYRIN REPEAT AND BTB_POZ DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="149"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="366"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="315"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="321"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="338"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4369009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="642" mass="72680" checksum="CA51E56B88F79B9C" modified="2023-11-08" version="1">MDVKKKYEIEAALATEAQAISAGKLKEENPLDVSDDFRRFCEACRRGDQKVCQEMISTGVNINARDKFDYTPLILASLCGHYEVIRLLLENGALCERDTFQGERCLYNALNDRIRNLLLSYDYAKDSSPLQPLAAHITSLLTRPTPKTTDITIAAYDQSFHLHKILLAARSPYFAKKLAEAPSTSSWKLPDNIPAQSLGVALQYLYFQEVSLRRALFGLSDEQELVVLNGIDKIGRQLEIERLFEDLTEVSDRRLLRQRRAEELERGRDQLESWFKDNVLKQKRVVDTDKVDSIQWDRDNSVFADILLRADDDEEELDQANRPSGASTSSTPPQRNTPGPLLGIPVGPVRSRSTSRQRAPRKSTIYPAHRAMLLRSEYFLTMFGSQFKEAQDTPHLQIVTIDCSPAVLETILIFMYTERADFGLDIAIDVLFAADQLFIDKLKQRAAIIISALGNGETSAVESDNPRGATDADEAVDIYEVIRAGWDTRVQRLEEFAARYIAYRLENYIDQPEFAELVRESASRVKARQETDTVEIIDDIRYYLSERFRLRFEDSGLDEMMDENAALNASTEGDLVDAMDDLALETKQQEESQLSVEQQIAAGAIRTLEGELVGDEFAQDAMNYQILLGKIDTLLERLNLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6X5</accession>
    <name>A0A9W8Y6X5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_005362</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6X5"/>
    <dbReference type="OrthoDB" id="5355161at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4369600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="342" mass="38457" checksum="0A853D411D005017" modified="2023-11-08" version="1">MFAGGQEPLEILIPRLLVPSISGVQLESGWFASPETWTIDKPPPDLLVNIDRFSSGDSDRILCHVHGWLVQWIEKGSNPFIHAHMYRYRFPQCIQDAYLSLSAYRQRTATNKHVICRIIEDRIQQLVRRGVSGLDATSGCGSDEASTFDALEYLARVQSLLVYQCISLFDGDIRLRHLAEQNLPVLEDWLYQLRALQARQSSCCGESLVYPSPQGFAASSKAIPPGNLLWYSWILAESTRRTWLVTSGVQGIYKLIRNNEAGCMGGLIFTCRNGFWNAPSAVAWEKQCSEVYAGMVRLTEVDKLLALVPPEEINDFAKFALEGTFGGEQMERWGVARAYGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y709</accession>
    <name>A0A9W8Y709_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005557</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y709"/>
    <dbReference type="OrthoDB" id="3797007at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="416" mass="46176" checksum="C25140B43251BEC4" modified="2023-11-08" version="1">MEPAANHVKEVPTLGDNGTSTKDTLMQGASQEADRIPSAIGENLGLAPTLSVSHDVQQGHKRKIGDTGSHSNDDTTPLKKRHVASENSSRPSHLDPEQAHVMSGNTLDVDEDIILEETASLIIPAEDSDLPQFQALYDAYHNSYTDSTITNANRMEHAKALALALLTHYYPASEDFIVEPSSLGPVAKHGMNFLHVADEAPAIQKNLLAKKLAKKGKKKAIPKSRKKEPAKVHEDHWDWIKPEDIAGFVVARKAKIVDKETGVVTYKHRPHTYLAIMIDTFDELPRFSKENITHRADALTDALCRIAKIQHGYGILIYGPRIEFYEYDAGKPWIYYESDDDDGEDEVQDIEPVMRLMSTEARIDEDLAMDMRTASLWMVEDAFELVAALEVTYADDVVVLGAGLEQHIEDADGMAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y739</accession>
    <name>A0A9W8Y739_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Replication factor C subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RFC5</name>
        <name evidence="7" type="ORF">N0V83_005996</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y739"/>
    <dbReference type="OrthoDB" id="761538at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000030">
        <property type="entry name" value="replication factor C subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000002">
        <property type="entry name" value="Replication factor C subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000136">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF1">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13177">
        <property type="entry name" value="DNA_pol3_delta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22534">
        <property type="entry name" value="RFC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="34"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00070185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4368914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="355" mass="40462" checksum="5F63572435157FEF" modified="2023-11-08" version="1">MALLVDKHRPRNLDALNYHPELTERLRALAQSGDFPHLLVYGPSGAGKKTRIVATLKELYGPGVEKIKIDARVFQTTTNRKLEFNIVASVYHLEITPSDVGNYDRVVVQDLLKEVAQTQQVDLGAKQRFKVVVINEADHLTRDAQAALRRTMEKYSPNLRLILLANSTSNIIAPIRSRTLLVRVAAPTETEICKVLSKVGKKEGWKEVETLNQRIARDSGRNLRKALLMFEAVHAQNEKITDQTHIPPPDWEALIEQIARQIVEERSPQRLLQVRASLYDLLSHCIDSTTIIKTLTWKLIPKTDDALKPEVIKWAAFYEHRCKMGSKQIFHLEAFVAKYMRLYESYSMGLDFDSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y784</accession>
    <name>A0A9W8Y784_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_005411</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y784"/>
    <dbReference type="OrthoDB" id="8061355at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF250">
        <property type="entry name" value="ABC TRANSPORTER DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="316"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="352"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="422"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="821"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1038"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="479"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4369649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1083" mass="119908" checksum="FB37CDFA61B556DD" modified="2023-11-08" version="1">MNLFLRQTWALIRKNLLLICIRRPIYTFIRALVIPLVIVLVVGYSKNFFASPQHWGVSSPHDVRSLKEGLGATPGHDIVGFVDNGMKDGEVAAVIESLSKEIRDAGKTPRTYNSSWSLAEECRTDTKGVSPCYGAVVFLSSPSEGSNESSVGYWNYTLRGTGSGNIDVSNDMNGPEKHLLPLQRALDMEILRQSRLNGTSQSLPDVDIILYTDQDQTALDDSRTSNYLMLAIYAFGPIFVFALVDLVYHMTSFVARERELGMSGLIDTMISGGSSVRGRIVRQIATYISFTVVYFLSWLAVGIVIAKVVLPVHSRALPVGFLILAGLSLTSFSLFGASFFKKAQLSGSIMTVIIVVGAILPVVIFEQTKVICAVLSIIFPSSNLSYFLTGLATFEAANKPVDMMNFTFESDSDNRNFRLPLYSHWVIVVVHILVFPALAFAVEHVLFSTASPHRRFAPPENSGDPTVVLSGFSKTYRPSILTRIFKRGKDVRAVQGLDLQAYRGQILCLLGPNGCGKSTTLNCISGDQKVSCGNIIIDPTGGLGYAPQKNVIWPELTVEEHIRIFSDLKCLSAVNEEVVHDLAKGVDLLKKLRAQAKTLSGGQKRKLQMAMMFAGGSAVCCVDEVSTGLDPISRRRIWEILLAERQRRTIIMTTHFLDEADYLADDIAIMYKGTLRAAGTAASLKHQYGDGYTIKLPYETDVNIQLPGSVQTEHSRHQTVYRVTSAAIAAGLTERLEEHQLRDYQISGPTMEELFLKVTGDKIHTSETVASKEETTNAKDESVTVDITDTEYELDAGRPISVFKQWSILFGKRLRILKRRYVPYFVAVAFAIVGAAIAPLLIKSFKEPIACPTLESLVDNSELRSDFGTSYYGSSPYGDSDTSSIYQKVYVLGPASKLNDSTISDRVEIMANVYSKNHTKSYSADYPRGYSNGSQIVEQMLLVNTYDEFSHAVQNNWKAQAERYALEDSFSPYSTLKGGIWLGDKESTPTIMANVRQIDYFAEITNFANLMFSGMPISTAFDNFASTTIPSLIDFTPLMFIVYYGLIMCWYVTFLILSNTEQVTQLFDGAYMILNGARADRFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7G3</accession>
    <name>A0A9W8Y7G3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tyrosinase copper-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_006185</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7G3"/>
    <dbReference type="OrthoDB" id="6132182at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1280.10">
        <property type="entry name" value="Di-copper center containing domain from catechol oxidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008922">
        <property type="entry name" value="Di-copper_centre_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050316">
        <property type="entry name" value="Tyrosinase/Hemocyanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002227">
        <property type="entry name" value="Tyrosinase_Cu-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474:SF127">
        <property type="entry name" value="TYROSINASE COPPER-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474">
        <property type="entry name" value="TYROSINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00264">
        <property type="entry name" value="Tyrosinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00092">
        <property type="entry name" value="TYROSINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48056">
        <property type="entry name" value="Di-copper centre-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00498">
        <property type="entry name" value="TYROSINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040995013" description="Tyrosinase copper-binding domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosinase copper-binding" evidence="3">
        <location>
            <begin position="249"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4369101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="332" mass="37655" checksum="A6F9FF631B7118CF" modified="2023-11-08" version="1">MAMGRTILFLCVLAVFPCAHLSYAQEGNEAQPQKCSKLLIRKEWRTLTHSEKTEWVGAVKCLASIRHERLSLTKDQTVLEGKRSLYLFDTSLRDTCGYNGPTPYWDWSRDHADLFASPVFGDSAEYGLGGTGDCDSSPEADCTVTTGAFAPSTGNFELAWPIPHALRRNLTLITGWFDHELPQNRTLGPEFVRNSTEQTTGDFFRFQYAMTLMHNHVHNFVGGDLAGDCPAGLREEDCKGMAISFTPNDPLFWLHHVQLDRLWSEWQRHHPFNFAAFSGMPLNPQNMTDPRYDLNAHADHHMSFDVQSVPVAPRSVFDTESWPLCYRYTDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7G8</accession>
    <name>A0A9W8Y7G8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_005533</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7G8"/>
    <dbReference type="OrthoDB" id="3942339at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0862">Zinc</keyword>
    <keyword evidence="1 2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="365"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4369769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="398" mass="44401" checksum="78EF268678E612A7" modified="2023-11-08" version="1">MDSDDEAWNELDDVQIAIGDCLNLDALSKLVDDMTHTLINIDAPDWYMNPDFGFLFGERSSYDSIAACSSLSFPPNLHDVLTSPVAPSIAFLKSLPLPAGDFWAIYVIVMEKDGCPTLVYIGSGTDAIQGVSARLPHYVPGCAAAPRFVRHAFRKGYHVAHRGLLCWTPLPNAGLVPRVRARFLALEALFTCLFFAAYAAVTDQYHEHLLRWEREAVEWGPLCSHISLKEDIKGDIYLSAEEVEIVAALRKKHRAQYIKDHRAARRGKDVDAYRAHERVTKNAWSARHRVALSETSAMNRQNAIAARRFHCDVCDMSLQSQHALDKHLGTEAHADAVDGVQKSEMSQYALNLKAQRAANKAACIHHCSVCNKSFENDWSLTRHKEGKRHIAKEAKASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7H1</accession>
    <name>A0A9W8Y7H1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cryptochrome DASH</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_006200</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May have a photoreceptor function.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </cofactor>
        <text evidence="7">Binds 1 5,10-methenyltetrahydrofolate (MTHF) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="5 7">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the DNA photolyase class-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7H1"/>
    <dbReference type="OrthoDB" id="435881at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003904">
        <property type="term" value="F:deoxyribodipyrimidine photo-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000719">
        <property type="term" value="P:photoreactive repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.579.10">
        <property type="entry name" value="DNA Cyclobutane Dipyrimidine Photolyase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014133">
        <property type="entry name" value="Cry_DASH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036134">
        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005101">
        <property type="entry name" value="Cryptochr/Photolyase_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002081">
        <property type="entry name" value="Cryptochrome/DNA_photolyase_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02765">
        <property type="entry name" value="crypto_DASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455">
        <property type="entry name" value="CRYPTOCHROME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455:SF22">
        <property type="entry name" value="CRYPTOCHROME DASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03441">
        <property type="entry name" value="FAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00147">
        <property type="entry name" value="DNAPHOTLYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="Cryptochrome/photolyase FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="Cryptochrome/photolyase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3 5 7" id="KW-0274">FAD</keyword>
    <keyword evidence="2 5 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cryptochrome/DNA photolyase FAD-binding" evidence="9">
        <location>
            <begin position="254"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="480"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="483"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="510"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="203"/>
            <end position="207"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="375"/>
            <end position="377"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="6">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="6">
        <location>
            <position position="362"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="6">
        <location>
            <position position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4369116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="540" mass="61126" checksum="7907E39161BE289F" modified="2023-11-08" version="1">MRAGRVGDVVSDILDWYSKGDVEKEENAKVTGIYMTKEDGTEELDDMNIVKTIAEEHNVDFEVWEDEKYFVDDRDLPYEKISELPNVYTTYRKSLEPLRDRPRNSLTAPTRLPPLPSVIPPQARPFTIPSSLKELKKALLVPLEKDSTYTLTHPPQWPVDVKSAHPFDGGETAGLNRISHLISSGAMSSYKATRNGMLGLDFSTKLSAFLAQGHITARQVHWAMVDFEAGRGAGQNLDGYGQGENEGTAAVRFELLWRDYMRLCMQKFGYRMFTLYGIQDHKGKGEQPQYSRRKRWRLLSRSNRAGDDPEKTLETFTRFRSGRTGIGLIDASNRELFVTGYTSNRARQNVASFLSSHLDIDWRIGAEWYECFLIDYDCASNWGNWQYVAGVGNDPRQGRVFNPVKQALDYDSHGDYIKAWVPELRGIKLTRPIGPGKEEVDQQRLMYLYQVWKLSDWEKSNLGLKGIDWVEHPLTKIQFSVGRGRGGPGDGKARRGGGGGGGGSWRGRGRGQDRNFRRMGNEEKAAKHEEGPIIVSSERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y882</accession>
    <name>A0A9W8Y882_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mitotic spindle checkpoint protein Bub3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">BUB3</name>
        <name evidence="4" type="ORF">N0V83_006542</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y882"/>
    <dbReference type="OrthoDB" id="10262475at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="305"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4368186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="396" mass="43416" checksum="F20C0ABED6A4164B" modified="2023-11-08" version="1">MSSKYEDLFADYGLLPLLLLLLLPCLLYLQSVQRITAPSLLLLQAQVQEGKLTATGQFELAQNPTEPISSVKFAADNPTRLLVSSWDRHVYLYDTHAEPGGKLLQKFEHRAPVLDVCFGRDENEAFSCGLDWEVRRIDLETGAQTIMSTHSKGVRNVLFSAAHNLLISSSWDSTLHLHDLSRPGDFASVRLPSKPFSLSASHTKLVVAMASRAVNIYELDQLAAAAKTGGGEDVGVAPWQQRESSMKYMTRAVACMPNDAGYSSSSIEGRVAVEWFDPSDESQSRKYAFKCHRQAVDGQDVVYPVNALAYHPIHGTFATGGGDGVVALWDAVAKRRIRQYQKFPASVQTIDFSSDGKYVAVGVSPGYEDAIDDVPDGVIKVFIRELSATEASGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y892</accession>
    <name>A0A9W8Y892_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycoside hydrolase family 2 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_006557</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y892"/>
    <dbReference type="OrthoDB" id="20872at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036156">
        <property type="entry name" value="Beta-gal/glucu_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051913">
        <property type="entry name" value="GH2_Domain-Containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006103">
        <property type="entry name" value="Glyco_hydro_2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006104">
        <property type="entry name" value="Glyco_hydro_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006102">
        <property type="entry name" value="Ig-like_GH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42732">
        <property type="entry name" value="BETA-GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42732:SF4">
        <property type="entry name" value="BETA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00703">
        <property type="entry name" value="Glyco_hydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02836">
        <property type="entry name" value="Glyco_hydro_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02837">
        <property type="entry name" value="Glyco_hydro_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49303">
        <property type="entry name" value="beta-Galactosidase/glucuronidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolases family 2 sugar binding" evidence="6">
        <location>
            <begin position="18"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 2 immunoglobulin-like beta-sandwich" evidence="4">
        <location>
            <begin position="320"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 2 catalytic" evidence="5">
        <location>
            <begin position="406"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4368201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="683" mass="76953" checksum="70857B7A8376EF99" modified="2023-11-08" version="1">MADYPRPDFIREDLTWTSLDGTWSFLFDDNDVGLTQGWHLNGLPDEVTVNPSNTNSAENTEAQSITAKIAAGTQELLQGNQFQSHEAVVNKTRAIQVPYVFQCPASTINEKGVHEVLWYERYITDIRKYKTTDSVDRVILRFGAVDYEAKVWVGGRFFGGHRGGHVPFDIDITDAFANGGNVQRLTVRVFDSAHDISQPRGKQYWKAQPESIFYTPSGGIWQSVWLEAVPSVRIGDASTGTVLRSNDIEGGQLYGSIKILGRPAGHKYSVELEASLGGILVSKTDQVELPRDSDTVSLTANMRLSNEQKSRLPNDIIDNAPLGDDSTWLNGVALWSPEHPTLYDLTIRLYDSTSSLLDTTKLTTGMRSLHWDNHTFRLNNQPYFQALFLDQGYWPDTFMTPPSSSALKTDIELSKRMGFNGCRKHQKVEDPRFHYWADRLGFLVWGEMANAYAFSAEYAERFDAEWRESVLRDRNHPCVVAWTPVNESWAYTDLAANTQQRDHIRSLYYATKVLDPTRPVNDNCGWEHVCTDLTTFHDYSDGDELTRTCSSLPGILAPKSNRPIFLPPIESLDPGSEHKDGAPVICTEFGGVNIARETADKAGDRDWGYTTATSSSDLLRRIEKMMMGIVNGGHICGFVYTQLTDIEQEVNGLYTPEREEKLDAREVKRIVEGVKTKYAEMHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8D9</accession>
    <name>A0A9W8Y8D9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">chitinase</fullName>
            <ecNumber evidence="3">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_005648</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 18 family. Chitinase class V subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8D9"/>
    <dbReference type="OrthoDB" id="73875at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF402">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="7 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="12">
        <location>
            <begin position="1"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="878"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="885"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="918"/>
            <end position="940"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4369884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="940" mass="102222" checksum="E684DBEE5584FF65" modified="2023-11-08" version="1">MNSYDTDLYPRVTALKKMNSGLKVFIAVGGWAAGGAGFSRMVSSPSSRSTFIQSALSFMRTYNFDGIDIDWEYPAAGDRDGTAADKENFVTFMQELKDACGATYGVTATLPSSYWYMKGFDIIQLEKHVDWFNFMSYDIHGTWDGSSPYTQAVVQPHTNLTEISQGLDLLWRNKINPAKVVLGLGFYGRSFTLSSSSCTTPGCAFSGGADAGECTKTSGILSNAEIQRLIKAKSLKPILDKEAGVKVSYDDEETLQLKKDFANKHCLGGTMVWALDLDDASTSTSLVSTQLQQLRDMGDDVDLNLKFAKAKLASTQKANNIGLATFWTDCQDDPQCPQGFTKLTLGHGKVFDSELNKYTADGCKGGPKGYMRALCVESNVVARDCDWYGKPKKCGSTCPAGYLLISQNTHPGGSSRYDALTPANPISCVLTTSGSGCKTGYFSSYCCSSIEASNLMDCSESAAGKALSGGLNLRAKNLGTFQNALGKGDADWGTLAECAAEAYNDGTVGELAALGVFSAGLYVLNHVAGHWVQNAANRWIYQPLRNLRYPSSASAVCTTTVTSYVEETRVSDVKTVKCDGNKWPNACYHYSSVSRENGLHTLVCPNKAIGGSNRPAVNAYRADRANTEWFKWVSNKPAVSAGVNKCNRDEYPPFRFLAAPNGNPGVVAPANYDQWIRFLPQSENGGAGQLWNGLCDKNGKKETKTQGGPINGQVCTEIASVTYKVKAMSMDFTNLPYRVDDGMPDNWCNLVATLRVDYGFNLLNADPWYNNRGSGFRGFRPSYLSYPPSLLTMGKTRPRNTLAKRLDSNQVDLDETIVDEGNSTRLATPEELLAENGLYKCQSENCEEEKELLNEFIIHLKGAATGEVVVEAVATLGPAEPSDGYTPSRSRKTGSASMPVNTAQPDALVEAEHDEAKHRRRHGHGHGHGHAHVHDRRSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8X6</accession>
    <name>A0A9W8Y8X6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8X6"/>
    <dbReference type="OrthoDB" id="3797798at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="330"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="404"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="414" mass="46845" checksum="2515523AF03AAA68" modified="2023-11-08" version="1">MSAYVPGTYGVFWTNARTADLAALPPYLQAEFLRIWKLGWLTGPKNFESPWDFYMGHYLLLNSVLWHHFGGGFNVPDSMVAALRTRLDFDDDGRFGIDTNVGDLVPSKERWPDPLLPYHGLEKVRLDFDAEQYFALFNVAVPPFNYQDNGVHGDNLYPDPFCHGAAALLLHTKDLTLHFGTAYKSAHPWYNVGEEKWCTSPTTTTAAPSSSLPGVIVEGEETVEPRLRPHVCVSGKVADWILEYAWHHRFLQHIPHIRITGDVQPWVREKWHAIFAAQAEHNKQVLAATSTATPATPATATPAPVGVLQRNTLLQPFAVHRPDIRAIEGIGKVRRNDDDDEDPADPADQPEWKPERYYPPKCECEIGCWRLVGGKVLEETREKSWEEMQEEQGEEGVEVVGNWDDGREEVRGVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y908</accession>
    <name>A0A9W8Y908_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peptide hydrolase</fullName>
            <ecNumber evidence="14">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">N0V83_005514</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M28 family. M28A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="14"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y908"/>
    <dbReference type="OrthoDB" id="10013407at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03876">
        <property type="entry name" value="M28_SGAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000054">
        <property type="entry name" value="Peptide hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041756">
        <property type="entry name" value="M28_SGAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF57">
        <property type="entry name" value="PEPTIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="7 14" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="11 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PA" evidence="16">
        <location>
            <begin position="111"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="17">
        <location>
            <begin position="226"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="466"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="466"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4369751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="485" mass="50994" checksum="F4A3B7CE28FFAB26" modified="2023-11-08" version="1">MLARRQYGSQKPLVTSEALQADITQENLLAGAQQLEDFAYAYEGRTRIIGSPGHNDTIYWLKDELEALDYYDVSLQNFFTVAMISGQINTFLVNGVNASGGLLEYSASGNVTAPLVVVANLGCNATDYPAAVSGNIALISRGSCEFGLKSALAGNAGAAAAIIYNNAPGGFNGTLGAPPRPEGDYVASISVSQEVGTGYVESITGGANITATIDVLTDIQNTSTYNVLATTKGGDQNNKLALGAHTDSVLAGPGINDDGSGTVGILEVAKALSKYEINNAVTFGFWAGEEEGLLGSTYYVANLPANESAQIRGYLNFDMIASPNYVHAIYDGDGSAFNISGPPGSAEIEAFFENYFTSAGQNFTATAFDGRSDYGPFLEVGIPSGGTFTGAEELKTEEEAVAFGGEAGIALDPCYHSACDNVTNLDLGTFELHGKAIAAAVATYSTSWEGFPARNTTVLRRSVVKARKTHQARHHRSRRSLPLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA47</accession>
    <name>A0A9W8YA47_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thiolase-like protein type 1 additional C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_004085</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA47"/>
    <dbReference type="OrthoDB" id="435240at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040771">
        <property type="entry name" value="TLP1_add_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919:SF139">
        <property type="entry name" value="THIOLASE-LIKE PROTEIN TYPE 1 ADDITIONAL C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18313">
        <property type="entry name" value="TLP1_add_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase-like protein type 1 additional C-terminal" evidence="4">
        <location>
            <begin position="420"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4372311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="519" mass="55818" checksum="20E70E9D7AA9C728" modified="2023-11-08" version="1">MAASETPIIVGVGDIKNRSTAVADAKEPATLMLEAIRAAIEDASSSSTSQLQSAIDSIDVVKTWTWPYPDLPGLLAQKLGVEEKTKWKRYSEHGGDKPGKLFDEAAKRVAAGECKVAVVTGGEALASLSACAAAKKLPPPGWTAPSEAVDSVFTPTGRDLGNNLGAIHKIGAPIHVYPLYENAFRAHRGQSLKENHEESAKLYADFSKVAAENKYAWNSGRADSEEVIGKVDKKNRMICYPYPLLMNAFNTVNLASAVILTSVSTAKALGIPSSKWIYPLGGAGTKDSDEFWKRPNFYSSPSISRSIDASLQISGVSKEDVDLIDIYSCFPIVPKIAAHHLGLPIVGGPKKLTLLGGLTSFGGAGNNYSMHALTEMTRQLRDGKGKNGLVLCNGGVLSYQYVVVLSKEPRKEGGYPRENPLPLQIIDVPAPDLVLDGEGEAVVETYTVEFNRDGSPLQGHIIGRLRSNNKRFLANHGDENTLRQMASGTGEVVGKSGWVWQDEKSRGRGLFAFAKPSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA89</accession>
    <name>A0A9W8YA89_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Restriction of telomere capping protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_003982</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in a process influencing telomere capping.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RTC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA89"/>
    <dbReference type="OrthoDB" id="128308at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039024">
        <property type="entry name" value="RTC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028094">
        <property type="entry name" value="RTC4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41391">
        <property type="entry name" value="RESTRICTION OF TELOMERE CAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41391:SF1">
        <property type="entry name" value="RESTRICTION OF TELOMERE CAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14474">
        <property type="entry name" value="RTC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01312">
        <property type="entry name" value="RTC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Restriction of telomere capping protein 4 C-terminal" evidence="9">
        <location>
            <begin position="389"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="176"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="226"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4372208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="548" mass="60812" checksum="C6D1CF31ABE0BAA8" modified="2023-11-08" version="1">MVIGLTKRGAPPLLRKVGGKRHATNEDHEEGAYEVRPNASNKSAREKEEEQSINAEPLSTDDELQQPPPRTSASSRPKIPLPAENRDGELKKPQRKASRKEPAIRAPTRGSYQSGKMDKAKRGIEDDKENATSTQSSVEASQDQGWGFDPDLSQPSSSKKQKTRYGTQPTAATRNIHVKKSLPKPRGKISYSRKGQPASQEDDGVSDVSIMSDAELDSALASVPKQVEDPELCTKVDKKKGSKDQNGGSKPTTLDDVELNNILDEPSKPARLLHQLGHWVDDQAPSSSQPKSSAPQEALENLHEYIEQLPQDEEEGTRCSLCKEPVELSDYWDFWKGKDRTVKNHTAFCNTHRKKSAHAAYLKAGYPSTIDWTALPHRIAAHKHALVQILNNTNTHNRPSTYRDRYAPIALTGKAASLCLPSHRKDLPAHLQDSLEDFHTLDDKSTYPGYYGPHGRRAITEHVMKLLKNDIKRCDDAVVQASGPAAFVQAVLVPEVAVLLIMEDCDEGMGMGMGREEEAERVRRTRMRWGCCLMRRLRIVLRGRGGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB00</accession>
    <name>A0A9W8YB00_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Small glutamine-rich tetratricopeptide repeat-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">sgt2_1</name>
        <name evidence="3" type="ORF">N0V83_003359</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB00"/>
    <dbReference type="OrthoDB" id="2335338at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044563">
        <property type="entry name" value="Sgt1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45862">
        <property type="entry name" value="PROTEIN SGT1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="77"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="365" mass="39896" checksum="072BB71B70123EF5" modified="2023-11-08" version="1">MGFTDKLKKKFARPNSVAGPSAGPSQYQSSFERPNQTYAYRPAPGQAGGPPAVPEVVQTPPASSNVPNPVSQQIVNAGLFRNAADEKTAAGDFVSAITLYEQAIKLTPDDPELLLCRAMAHRLSNPPQYQAALRDVNVAIQSNPTSWYAWHTKGELMGCLNDYDGAEEAFREAYASAAGMDKIKVQTSIGDIRRKKEMANTLPPQISTPPAPQPALMNQQPALNPQATQPSYYTNTQGNPFTLIFDPYHNSLSTVDSGADPKHDCLPKPVYSCTTSKNTISIRLYSSNSIYYIIDEPNTNFTTIHKLSFSTTAYTSYTEYPSACILARASKSSIRVSKFIHVSKLGHTSKGFVIWQHPSTQCACF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBM1</accession>
    <name>A0A9W8YBM1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_003708</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M20A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBM1"/>
    <dbReference type="OrthoDB" id="10059875at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF32">
        <property type="entry name" value="ARGE_DAPE-RELATED DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="6">
        <location>
            <begin position="193"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4373413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="424" mass="45802" checksum="1CA8779DA4FF93FF" modified="2023-11-08" version="1">MASKEDILNEIDANREEYIHLLQRFIQAASPNPPGDTTKASRVMIAFLSQHDISADVIAPQPHMPNIVAHFDGERGPGHRVVFNGHIDTFPANEPEKWERDPYSGHNDGSAIHGLGAVDMKAGTAASIIAFTLLKKRAQHLKGSVALTAVSDEETGGQFGTKYLLERSDNPALWRGDCVINGEPGGLQSVRFGEKGTLRLTFTVTTPGANGAYKNLSRGANIVAARLITKLQETVESISSDLPQDLKQHFANPHTRKVVDEIMGPGAASVLLEPTLNIGVLNGGVKVNVIPSQCIFEADIRLPTGLTRDTMLARIRDEVLKDFPEASYAVQEAASNPANYLAHDHHPLTKCIADAAEGVTGRRPVPLVGMGGTDCKFYRYLGIPAFVFGVSPKGMGARGEAVLIDEFMAVVKTHTLAVWDYLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC41</accession>
    <name>A0A9W8YC41_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aldehyde dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">HFD1</name>
        <name evidence="8" type="ORF">N0V83_003367</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC41"/>
    <dbReference type="OrthoDB" id="440325at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07135">
        <property type="entry name" value="ALDH_F14-YMR110C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000025">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000004">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF11">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0125">Carotenoid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="7">
        <location>
            <begin position="16"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR036492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR036492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4373076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="508" mass="55733" checksum="DD89559D8D563030" modified="2023-11-08" version="1">MATIELPSFKHTPLDDIASTTSQLRASFLSHKTRPLEFRIQQLRKLYWALKDNEALISEACKRDLGKPSFETYLVEISWCMNDCVFMANNLKRFAKDEKPQDIPMTNMLMGPRIRKDPLGTALIIGAYNFPFQLSVGPLIGAIAAGCTAVVKPSESAPYAAAVLEKVLAESLDPSCYTVIQGAIPETTALLDQKWDKIFYTGSEHVGKIIAKKAAETLTPITLELGGRNPAIITKHADPKLAAKRLLWAKTLNAGQVCVSQNCTFIDREMVPAFIREMQNALNEFYPNGARASPDYGRIVNERQFARLKKMLDSTNGRIVVGGTMDESDLFIEPTVVQVDSISDPLVADESFGPILPILPVTDLDEAIRIANSVHDTPLGTYAFGTKAEMEKILNQTRSGGASLNDGFFHASIPTMPFGGVGTSGQGAYRGKASFDVFTHRRSVTTTPSWIEGLLDVRYPPYSLKKQKKFAGMNEIKPNFDRSGRSIGWGGWLLGLLGVKGLVVLLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC44</accession>
    <name>A0A9W8YC44_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">TBP-associated factor 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_003591</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC44"/>
    <dbReference type="OrthoDB" id="21060at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045144">
        <property type="entry name" value="TAF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007900">
        <property type="entry name" value="TAF4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15138">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15138:SF14">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05236">
        <property type="entry name" value="TAF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID component TAF4 C-terminal" evidence="10">
        <location>
            <begin position="332"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="464"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="635"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="436"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
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            <begin position="464"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="635"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017306"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
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            <dbReference type="ARBA" id="ARBA00025346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031747"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4373297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="655" mass="70559" checksum="EA54E3D0EC59E2C6" modified="2023-11-08" version="1">MAYPNPNHNHNHNQYPPYGQQPPPVQTQNLNLNHNHHPQQRAFSPPSYHQSPTAMSPTMGSGIPPAKRQRLSPNPPSPSPYQSPFGAPTYPPSPYAPSPQASGYHSAPMSPATTQPPPFHQPQPYQHPNLAGGQHATQGSMPPPKVPYSKTGDSSELEKANARDLDVNNISDVLTGSGVNLRDEEDNLLHDYRSFNSQASAASASPHASFNNWSQLPGAGAFQGTGPLSQAMTAGKHEAEFLRKHEQAARILNESAQNPLTDPFLAANVLRHRIAKRAYEHGIQVNVDGLFDKIPDKTPRDATRTSQAGANGEQVVGLEAASLLNQNASLVEILSLLALAAEDRIRTIVEDSFALSQGRQNTSHGIIPPQLLDIAVVDDKAEQKMVAPVNILRTPWEAPDSAVSPTTTANKRRLLVDMHCSKTLTVTEPPNAARLPTPPSEAPPTPQRTFRTVNRVVAALEQRMKDDEKWEQERLRKRQKRLQGNSATPADTPAVPLPLPEPLTKKAQAAAKKQNQSDDVMFGKANETASMALGGKKKKYSWMTGGGGGGGGGGNASGPATPRAAAVNGGSGTNTPVVPQLDKALVSKKRSFGVMIEETDIGAKIQIRDLVHVLEDDGREKKSLSLILARLRNTDKDVNPRSAELDRRLPAAAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCJ4</accession>
    <name>A0A9W8YCJ4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_003558</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCJ4"/>
    <dbReference type="OrthoDB" id="412005at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029731">
        <property type="entry name" value="OSGIN1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15192:SF8">
        <property type="entry name" value="FAD_NAD(P)-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15192">
        <property type="entry name" value="PROTEIN CBG05349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="552"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="471"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="475"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00172"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ4373264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="928" mass="101772" checksum="97A88AE6206C80DC" modified="2023-11-08" version="1">MARSLPECVDTVIIGNGPSALILSYILHGHIPYYIGGHQDTILDAKLEKNPNLLHLTPDLYAHFLSSLRYSTQALPVNTLLDTLIRPNADTELNPKSCVEWRYEPERAVSHVAIGDTEQVGGQWADNPVSASADIGTLSYAEQLSLPGYSYADHLARGGKQDEPDFVRPSRTEFADYLRAYPEAVGIVDAIHTGLKVEDVYRAGGGFVVGSLGIRCRHLVLASGIFTINIPPPPLLAPVAQLDSIQEPLLVIGSGFSAADVIISAPPTRRIIHIFQWAPERRPSPLRGCHHTAYPEYATVYRQMKLAAIASAKSRPIKSPLLRRKSNPFFEKRNWDLYEGLPNAEVMNVSSPDNSSSATVTIRLISGEEITRDVGGLAYVVGRRGTLKFLSSDLQNEITSTTEPITTNGSSPYLISGRTLRAKAESSLEVAKNVFITGSLTGDSLIRHAVGGCVFAAGRILGEISTIYPSEDTHSPTALSPRSASPVSDTTFDRRRAKFDGSATSPCKPKTGRSTPGELTNGHADLHLDRRKLAKAVEVANAENRLWAESARHVKCDEQKPNCANCVKQDTKCEYRPSASRETSNGSPVAAFTPASSTEVTIARQGVPNGSTNAAPDITLNIPQLRLLHHYTTVTAKTLAVNLDGEAIFSTSLVHTAFEYPYLLHILLALTALHVNHIGGHSRAGNQDFLLQAERHHDEAISTFQATVRDIDDSNFRAVLMFAGSLFPYTCANAIAADGGLEHSFNSVLSNLILTRRVRPMVSGVYEAMRTSELGKLIPDDVKDIDWLKAEPPAHTELVQLRKFSEVVHHVYPPDIVDAYGQAIHLLELIFARAARSPSPASDGLLKIWIHFVTDRYVELLSEKQPGSLIVFAHYAVMLRRSEHYWFLEGVAEQILKIADALVPKEWNAWLDWPKEQIRNRSVISTPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCT5</accession>
    <name>A0A9W8YCT5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_003694</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5408934at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056416">
        <property type="entry name" value="DH_2_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056222">
        <property type="entry name" value="PH_23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056223">
        <property type="entry name" value="PH_24"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24340">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24344">
        <property type="entry name" value="PH_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24345">
        <property type="entry name" value="PH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DBL homology" evidence="2">
        <location>
            <begin position="708"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="934"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="1552"/>
            <end position="1691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="920"/>
            <end position="942"/>
        </location>
    </feature>
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        <location>
            <begin position="1085"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1141"/>
            <end position="1164"/>
        </location>
    </feature>
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        <location>
            <begin position="1194"/>
            <end position="1527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1532"/>
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        </location>
    </feature>
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        <location>
            <begin position="1708"/>
            <end position="1731"/>
        </location>
    </feature>
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            <begin position="1774"/>
            <end position="1820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1843"/>
            <end position="1870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
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        <location>
            <begin position="184"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="231"/>
        </location>
    </feature>
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        <location>
            <begin position="267"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
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            <begin position="370"/>
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        </location>
    </feature>
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            <begin position="440"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
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            <begin position="527"/>
            <end position="536"/>
        </location>
    </feature>
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        <location>
            <begin position="537"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="557"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="920"/>
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        </location>
    </feature>
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        <location>
            <begin position="1090"/>
            <end position="1112"/>
        </location>
    </feature>
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    </feature>
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            <begin position="1239"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1250"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1285"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1346"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1372"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1387"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1444"/>
            <end position="1477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1501"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1532"/>
            <end position="1546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1774"/>
            <end position="1787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1788"/>
            <end position="1816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4373399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1958" mass="213908" checksum="4D49DC2EB667DFE8" modified="2023-11-08" version="1">MPASPLPTDAEKLVGQKKNGTPTNKASATDKQNNMNGNDFAKAIGINKPANVRDKIKRWQQEVEPEVADAAKAGASAPAHVNTSTPSPRRQSTPNAKPLEAKPNWTPKHTPSRSVDVSPERPSSAKKPPVAHNVLDEDLMIATAPKKRVISDSHWRAKQSPPKDSARPAPKTIPNAWVRPSKIVKKDKPANDDEKPATPNTPPLNPLLPLVAYVGKSTGQKKHPQRPRRPSKPSSSENEQRPTSSGSGSGKDPKSEDNVDAPQSPSFDKEKTEIVKVRRRRRAQTSPRASLSADDATPYAQRPARRSDTSLSGDPMNIVTVEYDEESKITSPRDNEIRERRRRRRPRSQGDATADESPRDPTNANPTPKTARRRSRRSYPKSDEEAVVPPAVESASSTPPNKAFPNRLEAWLSGTPDPFVDSESRRRRKSKEPVSTLETPPKEDKSDVTKSDVTRSTDKTEDEPVEEPQLRRSGSGSRRRRRRRSREMRVDTQIVDEPPSTVVSDSTVETPAKDEESQVSLTPTPTLRRRGAKRSQHSPTKSRSMSSPLRETTSVDDANKENIKDADDASSAFSSSVDASAIDIENIPLRPRPLAMRHIFPSTGKRLSTIASVESFATKVQTAPPSEAPGSEAVTTISEETRTIPEVPASELGSQLTAETSTIVSRRSTRRKKLANHADLISVLSMPKAGTKSIVSARSIRTNRSRLATATIGDIMRELSSDETKYMRELRTIVDGVIPVLLSCVLSKSDSAVAAGLFSRSANTDSSDVTKPIVDMGVCLERLKTLHKRVPKEDSDAFVSWAQSAQRVYSDYIAVWRLGFQDVVISLAPADDDPFKPAKVVNGPEDGAPWDEGMPRNAEGYVINGDGERVDVAYMLKRPLVRLKYLAKTLKGINHVKPSERAEKVSSTFQDLMAAARKRSNDEQARLEDEAAAAVDPTRARDPRSLAPLAGVRVDPTRCVRARDHFDLHLYHSSGQEVSCRVEILLRDDAPGKGKGGDLLFCEVDPTGRWLLLPPIQLNRVSARNGDLKGEIIIMIRGFQNDGSEWSEVMSLTADDEQAGFEWVQMLGLHPIPLQIAEARKNLSTPVKAPRPASSHASSSLLSAATASTPPLKSRTPSPREIEIPIGEQHTEVSKVWHYDTPERRPRSHTASPITPPSVDDSQIGITRDVNDKLSPEAQQDLDRTPRSLNEALEMAGAGSPNVLKRTRAKRVSRNATSSPSTPRPSREITLDDPTELVEEPHTIEEEIKPRRKLTKKRPQSLPAAPSTVSQSSKGFSVWMPSEVGDSDESDDDERTVEEEPVSPPGSLPTSSQRPHMHRRVSSVPSMDLPSIPRLRKTSQPTTPVKDAEAKLEKTRDPSASAPSKLQKKRREPVLEEIKDSQKGVEKPPPVPPHRSPSPATPVTLKGSETPVFTPTLPGFKNKRRSSSPLKHEYEPSTCTESSSESEEDIASEEEDDDEGDESLTSESSEDELDDDVPMPLMPIDYTRSRGYAARPYSAGRDSRRRASTETPEDDAKKFPKVSPPASIYTLPNGTITPSQSASNTPYRAVPQGSGRATKAIASIFAWSDAGRWDTLHPDECSVVVTPGKIEVFEITANHSKPFMADGDEIIQPEGRAPLVAVELTPLVPLRKSTAIDISIRSPPTADSQIRTGNNIMLRSRNATECAQLYAMINQSRINNPTYIALQNARGPYGHTSWAEAMDRQNAARTNNNGSSSGWLGGTLGRRSSYRKTSTRAASISAATESSVGTMNTALRSALGRFSFGKSGVFSIRNSTLGSRSTGSSFDSGENSNPGSGSSTPNGAGEAGRAPGAPAGITNTKCRLYERETLKKWRDMGSARLTIMLPSPHPSTPSSPTNPLQRAPGKRDHTQERRIVVTGKTNNEVLLDVTLSESCFERVARSGIAVSVWEDTVGEDGEVGRVAKTGGVSGARARVFMVQMKSERECAYCFSLLGKLRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD06</accession>
    <name>A0A9W8YD06_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Centractin- actin- protein of the dynactin complex</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ARP1</name>
        <name evidence="7" type="ORF">N0V83_003332</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the actin family. ARP1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD06"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10216">
        <property type="entry name" value="ASKHA_NBD_Arp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000015">
        <property type="entry name" value="Actin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000502">
        <property type="entry name" value="Actin-Related Proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4373041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="379" mass="42203" checksum="DCFCBDCD0127CE29" modified="2023-11-08" version="1">MGSRLHNAPIVIDNGSGTIRAGFAGEDVPKCFFPSYVGRPKHVRTMAGALEGDLFIGPQAEQYKGLFKINYPLEHGIVTDWDDMERIWQYVYTEGLKTVSEDHPVLLTEPPLNPRENRDTAAQILFETFNVPALYSSVQAVLSLYASGKTTGLVLDSGDGVSHAVPVFQGFAIPNSIRRIDVAGRDVTEHLQQLLRKSGRIFHTSAEKETVKKIKETTGYVALDPAKEEKDWSGSSRSEGKSVDYTLPDGQKLKIGAERFRAPEILFNPEIIGLEYPGVHQIVVEAIHRTDMDLRKALYGNIVLSGGSTLTKGFGNRLLHEVQRLAVKDMRIKILAPPERIYTTWTGGSILAGLSTFKKMWVEKDEWQENPDIIHTKFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD34</accession>
    <name>A0A9W8YD34_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002657</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD34"/>
    <dbReference type="OrthoDB" id="1681166at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="288" mass="31501" checksum="277CF26D74A30BB2" modified="2023-11-08" version="1">MSTTSLNSKSPPLPRSSHSPRSPPLLHLEEPPDDEPYEQQQPDDAADEDEIPYPEPSSEQTLLPPPNFRPFFTLIEDTTLGEHYHPYVHYIFADDDPVIVTAASMRSLGLDDTKYLPQHTPEHEERQQAQETQDEDEDNSPVESPLPPPIPGVKEHYLIVDVGADGRTIVDAQSLSPEWQITDTGVRTAPSFDESSPDQGCMLRIEGVEIPKKSKGKGKGQVGQDKLSEAREQSQGDIFGALDGLVHSVEGGLDVARKISGTPAKETEENTVVGAPAEEEEGSRPGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD82</accession>
    <name>A0A9W8YD82_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_003941</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Part of the multisubunit transport protein particle (TRAPP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus</location>
            <location evidence="7">cis-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAPP small subunits family. TRAPPC4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD82"/>
    <dbReference type="OrthoDB" id="246406at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14856">
        <property type="entry name" value="TRAPPC4_synbindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.70:FF:000007">
        <property type="entry name" value="Putative sybindin-like family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007233">
        <property type="entry name" value="TRAPPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249:SF15">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04099">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01399">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5 7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="60"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4372168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="158" mass="17572" checksum="CBD6FA8D0C9C9C74" modified="2023-11-08" version="1">MPGVVFALFVISKAGGLIYNREFHTGMSKLTSNDYLMLAGSFHGMHAITANISPVPPASNPSSSTSTSSQTPTTFPVRATGLEVLESSHFRIQCFQTQTGTKFLLFTEPQQPNVDTMIKKIYELYADYVMKNPFYTVEMPIRCEKFDRGLDGFVKVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE31</accession>
    <name>A0A9W8YE31_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aspartyl/Glutamyl-tRNA(Gln) amidotransferase subunit B/E catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_002456</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE31"/>
    <dbReference type="OrthoDB" id="1722066at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="GLUTAMYL-TRNA GLN AMIDOTRANSFERASE SUBUNIT B MITOCHONDRIAL AND PROKARYOTIC PET112-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659:SF0">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aspartyl/Glutamyl-tRNA(Gln) amidotransferase subunit B/E catalytic" evidence="6">
        <location>
            <begin position="73"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="388"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="32"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="388"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4375370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="435" mass="47903" checksum="97ADF8725453F145" modified="2023-11-08" version="1">MRSRSQLEPTLLPARTATRSLQTESQPPQDDTPFRKALKDAAKQQKRQRGAASSSLKILNKGRDARLEKWELTVGIEVHAELNTARKLFSSAATSISEAPNTHVSLFDVAFPGSQPHFQEETLIPALRAAIAFNCQIQHKSSFDRKHYFYQDQPAGYQITQYYEPFATDGKLFLHPHDFPPGAAPQTCPIEIGIKQIQMEQDTAKTIQQPPSTHLLDFNRVSHPLIEIITLPQIHDPMVAAVVVRKIQTILKSINACTAGMELGGLRADVNVSVRERNGKQSDANKSYHGVTGLGQRTEIKNLASVKAVEDAIVAERDRQIDILQSGGSIEGETRGWTLGSKTTKRLRGKEGEIDYRYMPDPDIPPVIIGEVNNIPPYNWLGISANQNRTSSTTSRTPCPNSPTAASTSSSATTASQPKMPSPFYPLTTATAWTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEA9</accession>
    <name>A0A9W8YEA9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Conserved oligomeric Golgi complex subunit 6</fullName>
            <shortName evidence="10">COG complex subunit 6</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">Component of oligomeric Golgi complex 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">COG6</name>
        <name evidence="14" type="ORF">N0V83_002176</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as a component of the peripheral membrane COG complex that is involved in intra-Golgi protein trafficking. COG is located at the cis-Golgi, and regulates tethering of retrograde intra-Golgi vesicles and possibly a number of other membrane trafficking events.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Acts as component of the peripheral membrane COG complex that is involved in intra-Golgi protein trafficking. COG is located at the cis-Golgi, and regulates tethering of retrograde intra-Golgi vesicles and possibly a number of other membrane trafficking events.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the conserved oligomeric Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus membrane</location>
            <topology evidence="1 10">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the COG6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEA9"/>
    <dbReference type="OrthoDB" id="272987at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010490">
        <property type="entry name" value="COG6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048369">
        <property type="entry name" value="COG6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048368">
        <property type="entry name" value="COG6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21506">
        <property type="entry name" value="COMPONENT OF OLIGOMERIC GOLGI COMPLEX 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21506:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20653">
        <property type="entry name" value="COG6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06419">
        <property type="entry name" value="COG6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01087">
        <property type="entry name" value="COG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Conserved oligomeric complex COG6 N-terminal" evidence="12">
        <location>
            <begin position="59"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Conserved Oligomeric Golgi complex subunit 6 C-terminal" evidence="13">
        <location>
            <begin position="203"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="661"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="663"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4375094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="694" mass="77381" checksum="4207E00280315CF3" modified="2023-11-08" version="1">MSASYFQERVNSASQNGLSPASPSVGPASRGANALSSRITSVLSASYADLEIRDALETLDSRQVHNSAETRRQIRLDVQKEVIQCNGEIVKDFGEVAEQLKRIGTAIKSLNNCCADMRNHIAAANQETGPVLEEATSLITQRKQVEAKQQILHAFSSHFLISDDEANVLTSTVEPVNEDFFQGLTRVKKIHHDCQVLLGTEDQRLGLEVLEQSSKQLNAAFQKLYRWIQREFKTLDLENPQISASVRRSLRVLAERPTLFQSCLDSFAEARETILSDSFHAALTGTNSEEHIATKPIEFHAHDPLRYVGDMLAWAHSTTVSEREALENLFISDGDEIKRSIQAGLESEPWLKEDGDKEVFDGRKALNQLVSRDLMGVARLLRQRVEQVIQSHDDASLAYKIANLIGFYKGTFVRLLGSDSEVLDVFNTLNTSAMRQFRANMRDYVANVQSDLAVAPADLSPPDFLNEALQMLRVLAKSYDTSVAATDDQGQGFQTVLAEALDPFLSGCENLQKGMQQPDSAIFAINCLFAAKEVLASYRFASERVSEIDETIDEQVANLTEYQHQYFVHESGLITLLEALSTINDAEESLKTILDLDPFKPDALVAASQQLDEFLPSALIDASENLKKLRNRKMIQDITEEAAGAFCEDFERVESKMVASDDLTYDEDKEDEEQEPGLRDLFPRTSGEIRVLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEK0</accession>
    <name>A0A9W8YEK0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_002491</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEK0"/>
    <dbReference type="OrthoDB" id="408702at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF131">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="78"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="271"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="328"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="358"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="394"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4375404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="408" mass="45351" checksum="C8FE329EE89F3E61" modified="2023-11-08" version="1">MADTAGTARVQADLCHLSKESKRVEVFLYTGIVYGITSLFVFLRIAGKLVSKRLSLDDYIVVAALILAALPLGCVLAILVASSTYVLVLGLIKVSLILFYLEIFKTRRFVISAYVLLGYIIINSLIIFLMTLFMCRPINSFWNRDIKGKCMDQQSLGYANSASAIFQDIMLLTLPLVFIRNLQMKRYRKLAVGLMFAIGTFGCIATIVRLQALLAFKVSIDPTWDYVPVTIWTEVELAAGFVCACLPSIRILVVKILPKSFKKFFSQITHSSSKGNSRQKQSTTPQQASWKMPSDWINISHGDDEGEVATGPRKSYFGGLWTRRNSDAQSSVRRLGSRMSNHSESSSTAATRPAQEAVELREMRASEAVRGPVKNSPGGEERALPEIGCLPERNYSDLDLARHTSRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEQ0</accession>
    <name>A0A9W8YEQ0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LysM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_001342</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEQ0"/>
    <dbReference type="OrthoDB" id="2281372at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052210">
        <property type="entry name" value="LysM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34997">
        <property type="entry name" value="AM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34997:SF2">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0843">Virulence</keyword>
    <feature type="domain" description="LysM" evidence="5">
        <location>
            <begin position="111"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="5">
        <location>
            <begin position="197"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="5">
        <location>
            <begin position="280"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="45"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="259"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4376061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="328" mass="33895" checksum="D11D27A908C9D265" modified="2023-11-08" version="1">MLGDNYATLEDFRRWNPTIGANCAGLTSGRSYCVEALFEVIPTSSAVPTSKPTSKPQSSSTKAPTTPAPTPTAKPTPSSVKAPVTSSTTTPGNGIETPVPTQPNIVSNCDKFYLVKSGDQCASIASQFGITLAKFLAWNPSAGSSCGGLWADAYACVSIIGEDATPIPSATPTPTSPGNGISTPQPTQPNLVSNCDKFYLVKSGDTCANIASANGITVAQFTAWNPAAGSSCAGLWADAYACVSIVGHEPSKPTPTPTNPGNGVQTPTPTQPGMVTNCKKFDFVKQGDTCDAITKRNNISLANFTKWNTGVGSTCTSMWAESYVCVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEQ8</accession>
    <name>A0A9W8YEQ8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001146</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the avfA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEQ8"/>
    <dbReference type="OrthoDB" id="419598at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004074">
        <property type="term" value="F:biliverdin reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042602">
        <property type="term" value="F:riboflavin reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051606">
        <property type="entry name" value="Polyketide_Oxido-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43355">
        <property type="entry name" value="FLAVIN REDUCTASE (NADPH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43355:SF2">
        <property type="entry name" value="FLAVIN REDUCTASE (NADPH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="2">
        <location>
            <begin position="11"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="223" mass="24285" checksum="503A74A5C561A81A" modified="2023-11-08" version="1">MSTSKRVLIVGGSGRTGQLVIDELLARGHEVTALVRKPESLEAKAGLVTVTGTPMNKEDVRNAFAQSNPEVVVVTLSAPRAGDSPFAAVISPPRLMADSNANVVAVMKEFNTPKIVIMQAFGVGESWMNMHWVMRMLMKKSNMIYQYDDHNLVAKEVKASGENYVFVRPSRLEEGEAKEIKEYADDGKGVPMMGAITRGSVAKFLVEATESRTWDGTAPVITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFB8</accession>
    <name>A0A9W8YFB8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">O-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_001674</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFB8"/>
    <dbReference type="OrthoDB" id="2410195at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF12">
        <property type="entry name" value="STERIGMATOCYSTIN 8-O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="6">
        <location>
            <begin position="87"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="5">
        <location>
            <begin position="186"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="403"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4376391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="49799" checksum="7785FA6C59F7080B" modified="2023-11-08" version="1">MKAEQQSTLLELATEVQQLTTQIVNDLTEKKLKEPTFETDSETIPETPEQIGFRARLNDSARDLLRLVNGPRNDARTFVCYLYDLAAWQVACEFNFFEAIPETGTANIKEIAEKSGVDEDRVGRFLRMLATDRVFEEVEKDVFKHTSRSVLYLKDKQWRDVMHYQVDEFFKAASETSASIKESPSVTDGQRNAFVTRHGTDLFQFYKNDALRAARFASAMAGVSRLERHFESLKESFPWDQVIKGKLIDVGGGSGHMSVSLARAFPNLECIVQDSLTMLSSASQNDFSDLNGRVTFMPHDFFTTQPVSGAAAYLLRYITHNWSDEDCIRIFRALVPALEKSSPGTPLLINDVVDIMMMLVLGAKQRTEEQFRRLLSDADSRFKIRAIHGKGNMSLIEAYLDTDGAPRTEGPEDDGVLVDKSASGQDQTDSVIAGQQKRTGTRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFD3</accession>
    <name>A0A9W8YFD3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002926</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFD3"/>
    <dbReference type="OrthoDB" id="5427526at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="290"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="328" mass="35538" checksum="1729A8FB3959929A" modified="2023-11-08" version="1">MSSPPQTPTHRPVSSHRRNPSSLDMSYNTPSRRQSLNRRSSGYSPITPRSSHEFEHSSPAHHFDGGGDGNGLGNLADELGEIWDDDEEAMDGEFGEELDIPQDELANIGTAIEHDGSAWVDNTVNLNGVRDSGVAMESSSPARLSPEAATKTRKHTRQRSLYDGSDYGEDSDFENEGISPALESRMAAVESLARRGMEENGSASDQVVKRVTEQLRDLGSQIAIENGATRLKTAHDALTTHLTHQSRTLTSLAASFSGPRPIIPDSDTIDTLLPLIQSTLELLPQSSTEPPNLSLPSRSRPANFSSTSPMSATHCTCRGKPQQTPLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFP5</accession>
    <name>A0A9W8YFP5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_002836</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays an important role in cellulose degradation. Shows hydrolytic activity against several glycosidic compounds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFP5"/>
    <dbReference type="OrthoDB" id="65569at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080079">
        <property type="term" value="F:cellobiose glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000011">
        <property type="entry name" value="Cytosolic beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF134">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12600)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4374097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="492" mass="56356" checksum="A527B46DD05837AE" modified="2023-11-08" version="1">MTWTHTFNSSPDADLKGALRKDFHWGYATAAAQVEGAWNKDGKGESIWDKLCHTPGKVKDGSTNEDAARSYDLYKEDVALMKSYGVNAYRFSLAWSRIIPLGGADDPINERGVQYYNELIDELLANNITPFVTLFHWDTPQALEDRYGGMLNQEKYTPDFLRYARVCFERFGGRVKHWITYNEPGVYTLAGYAAGVHAPGRSSFRDRNEEGDSSTEPFIVAHTELVSHAKAVKIYREEFKPTQKGVIGITLHGNYSEPWDVNDPKDVAAAERARQFEIAWFADPIYGSGDYPQCMRDQLGDRLPTFTEEESTLVKGSSDFYGMNTYTTFFVKHLNEPADINDHKGNVEKLDTNSEGKSRGPESDTYWLRTAPEGFGKLLRWVWNRYHVPIYMTENGTTAKGETAPTEDVLNDTFRIDFYNGYVRELAQAVQDGVDIRSYFGWTFTDNWEWAAGYTDRFGVTYVDFDTPEKKRYPKRSAYFLRDYFKHLIAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGA2</accession>
    <name>A0A9W8YGA2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kti12, chromatin associated</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">KTI12</name>
        <name evidence="4" type="ORF">N0V83_001562</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the KTI12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGA2"/>
    <dbReference type="OrthoDB" id="9972657at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002734">
        <property type="entry name" value="Chromatin associated protein KTI12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013641">
        <property type="entry name" value="KTI12/PSTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12435">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08433">
        <property type="entry name" value="KTI12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4376279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="296" mass="33495" checksum="A692BFD0D1DAB06B" modified="2023-11-08" version="1">MPLVLISGFPSAGKTTRALQLKEYFETRIASSSADARVARLKVHLINDQTLGVARSVYHTAKAEKDARAEEYSAVKRVLSRDDIVIADGMNYIKGFRYQLYCEAKALQTPSCVLHIGTPAERCRENNKKLLADKDVDGGYEEDDFENLIFRYEEPNGMTRWDSPLFIVVEEDETPPNEQIWDALIGSDGKMKLVKPNLATVLKPATEQNYLYELDKTTSDILAQIMTYQKDHAGEGGGEIAVQEVAKAIELPATPMTLPQLQRIRRQFITMNRQHSFSKARIKEVFVDYLNAEFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGD5</accession>
    <name>A0A9W8YGD5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001607</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGD5"/>
    <dbReference type="OrthoDB" id="3796179at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="275" mass="30829" checksum="5BB80CBB92B2EA35" modified="2023-11-08" version="1">MRTNYRKTRKFPKTFTKAELLEVLEQKCGDALRSFPPPETFEFLHRGNRIALKVGNGIKWKRNVKKNLYLEDCETTSQATVRAAPPPLPGEVRLTSGKAGPSTSTFIHGKAAEDDEKLQTLYQNLSYTTFNDKPLNEKIKNSRGIKGDNVFGKLTANGILKSPFSNTIRVLYRFLLVYCDHKLNFEEFKIARSRVGGVNTLVRVLEEIAKRTSAVDGSEDSNATNETGFTGDFKNDSEEGSKNDAQLFAREGKGKKRALETVNDEGMALKKARKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGK8</accession>
    <name>A0A9W8YGK8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGK8"/>
    <dbReference type="OrthoDB" id="5428040at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="504"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="596" mass="64229" checksum="C3090C4F5EE194E7" modified="2023-11-08" version="1">MVDGWATRSVAVSTLILRTAVDLQAAIASAILAALLLESKAGVHLYQIASMSPMRAGTANPWTFASCMFKDLWRLTAQYRRNYQICIMAILLLITTSVLQFSSTILLSDLKSGPLVGHNIASEVRVGLSYVGETEKIPRDSAWTTNPPSFPAFGEYAESPASDNSGVVDTGVLLRAFLPYATSESRQRLSDYHGNALILDARVSCQAPTLTGFNGTGSTALNRQLTGVVAPSKNVTMLQNITATPFNCTVAWEGQVTICQLAQPKGAFTGSLASQFLGSTTYGTAFLIINASSQASAKDEWLEVTARGSQGTNTTAQISMSLCFAPWDAAVLDVSLTSKSNRTEAALRYWEGFQTLDVLSYLIPSAGKNSRPVLDMQKPRSFLGDRPPPYRRPVVQSDMGGSSAAVRGTIDPLPGNWSAFVAGSPLVSIVDGFEVQPTQAISADPALAAIFTSATKAGHSIEWALSSLLTVLSMTNYYGQQPAFDRLDNATVSFFEDVLYPRDYVGFTTLMWVLVTHFCLMAILIVLFVRNTRLTLIGNAWSAFAQVAESHDVKEHVTNANLKNDSDIFKDLKGLQKSNLRARIVARGGGAEVVVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGL9</accession>
    <name>A0A9W8YGL9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_000421</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGL9"/>
    <dbReference type="OrthoDB" id="269227at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.450.10">
        <property type="entry name" value="Glucose Oxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027424">
        <property type="entry name" value="Glucose_Oxidase_domain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF201">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE OXIDOREDUCTASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00623">
        <property type="entry name" value="GMC_OXRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0274">FAD</keyword>
    <keyword evidence="3 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040927789" description="Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein" evidence="9">
        <location>
            <begin position="17"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="10">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="11">
        <location>
            <begin position="300"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="534"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="577"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4377594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="599" mass="65178" checksum="254847D102943D9A" modified="2023-11-08" version="1">MFCSLPFLTLAQVVIATCTPTTFDYVIVGGGPAGLVIANRLSADSNVSVAVIEAGGSVYNNPNVTGLPRSIAEFSPGIGTSVDWSYVSAPQKYMFNRTLPYSAGKALGGTTTINGMTYLRAEKAQIDAWEELGNQGWNWDSIYKYYLAQEGFQLPNEEQRENGATYRDKAHGYKGELSTGFTPYLVGQGFFDILKTTSEELDYPLNIDANNGTMRGFNTWPMTLDASKAIREDAARAFYYPIAESRPNLHVFLNTTATKIIWDDTGYAVTEIVAAGVEVVTSGNSTEVIRATKEVIMSAGSIRSPAILEHSGVGNKAVLEPLGIETVISQPHLGSNLHEQPNIGIVYASPTNWTGYPTFIDYLTASDLFGDDLPALAKEIRANISDYADTILAEYASNTTTMEIQEQLLKHQVDLIFDPKSTVPLAEILWFPVGNNIVAQFWNLLPLSRGSIHITTADPLMPPSIDPKLFQLPIDTFVLAAISIHVRDFFTTAPLSGHVTSELSPGFEAVPRNASWRDSSWETWMKNTSSTNTHPLSTCSMVSKELGGVVDKEGKVYGTKNVRVVDASVFPTQISGHLSASVYALAGKFADMILGKIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGP9</accession>
    <name>A0A9W8YGP9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GST N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_002020</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGP9"/>
    <dbReference type="OrthoDB" id="2309723at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03190">
        <property type="entry name" value="GST_C_Omega_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047047">
        <property type="entry name" value="GST_Omega-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016639">
        <property type="entry name" value="GST_Omega/GSH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419:SF23">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419">
        <property type="entry name" value="GLUTATHIONYL-HYDROQUINONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015753">
        <property type="entry name" value="GST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="48"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="66"/>
            <end position="67"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="3">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="3">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4374942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="273" mass="31467" checksum="7E7A5E4FDD230999" modified="2023-11-08" version="1">MSIVRPYPKSETGWRFDETYPNATPDHLFNSRFMHQLYFRDDPAYKGKYSVPLLWDKKTNRIVNNESAEMLRWLPHAFNSILEEDPGDVEGLDFYPADLRKKIDEVTPWLTSLICSGVYKAGFVRNQDNYEKNVVPLFAALNNLEALVYANGGPYILGTKLTELDLLAYPTIVRFDTVYAQHFKTNLGMIRHDYPVLNNWLKNLYWNVEGFKESTDFKHIKENYTKSHGDINPLAITPLGPYPDVEEGYASDWSKLKPGKVAHPLVLEAQSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH98</accession>
    <name>A0A9W8YH98_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_000318</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH98"/>
    <dbReference type="OrthoDB" id="5393606at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF157">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="3"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4377493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="166" mass="18249" checksum="EF6097D444DDE60C" modified="2023-11-08" version="1">MVGYSYSISDFVSDGLILLIPVPFIWQLQLPLGRKLAVLGVFLLGMLSSGASLVRLAWMTYAQKVGFGDQTDEELMITTELYWMQLEITLGLLAACLPTLRGLCKTKSVDSVVRGVRSLFSVRSGSSSGSFKNSLENGRDSDEKSRQITVTVNNDIRYASRGSDRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHR8</accession>
    <name>A0A9W8YHR8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha,alpha-trehalase</fullName>
            <ecNumber evidence="3">3.2.1.28</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ATH1</name>
        <name evidence="10" type="ORF">N0V83_000995</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:32675"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="3.2.1.28"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 65 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.28" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHR8"/>
    <dbReference type="OrthoDB" id="200349at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004555">
        <property type="term" value="F:alpha,alpha-trehalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005993">
        <property type="term" value="P:trehalose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000032">
        <property type="entry name" value="Vacuolar acid trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.40">
        <property type="entry name" value="Glycoside hydrolase, family 65, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037018">
        <property type="entry name" value="GH65_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005195">
        <property type="entry name" value="Glyco_hydro_65_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005196">
        <property type="entry name" value="Glyco_hydro_65_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11051">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11051:SF8">
        <property type="entry name" value="PROTEIN-GLUCOSYLGALACTOSYLHYDROXYLYSINE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03632">
        <property type="entry name" value="Glyco_hydro_65m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03636">
        <property type="entry name" value="Glyco_hydro_65N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040808190" description="alpha,alpha-trehalase" evidence="7">
        <location>
            <begin position="25"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 65 N-terminal" evidence="9">
        <location>
            <begin position="49"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 65 central catalytic" evidence="8">
        <location>
            <begin position="401"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1008"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1008"/>
            <end position="1019"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4378162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1028" mass="112295" checksum="70C579551A19C483" modified="2023-11-08" version="1">MPWLNLRRAAFAALFATTTRLVCASTSSEHDQFENVTWDDDNWVISTHTLDQGHYQSRLSLANGYFGINVAAAGPFFEVDEPVDGDNINGWPLFSRRQTFSTIAGFYNSQPRTNGSNYPWLYQYGWESFVAGIPHWSGLQVEANGELLNASVNPDHISDFIASLDVKAGIANWRYNWKPGGSGDGTIDVDYQLHLHKLYVNQAAVRLRLTASRDVNVTVYDVLDGDCAVRSDFVDKKYEEDTPTIFTAVNPGNITNVTAYIYSTLSGDDWVDFDTRAEVQEGFFSTGNGSTIAQSVQVALTAFRPTEISKFVGIASTDAFTDPQDIAGNASVSGAQEGYYKLLHSHIEEWASILTPDSVDDYHLPDGSLPDDPDIQELQILARTNPFYLLSNMVGPNAIAAAGNNTRLDTHSVPVGGLGSDSYAGMIFWDADVWMAPGVQVSHPQHAQNVIKYRVAHFDQAKKNVDTAYQSSKNQTGRFTPGGAVYPWTSGRFGNCTGTGACFDYEYHLNGDISLAFNNHLIITGDGDYFKETLLPISNAIAHFFGEVLDYNKTAGTYELWNATDPDEYANQVNNVGFTTALIQRHFSETNEFNAWFGLGQNATWTEKSEQMRLPINENAGIIVEYTGMNGSVEVKQADVVLVDDLLHYPNPYSLANLDYYAAKQSLDGPAMTYSSFAVVANEVSPSGCSSFTYDVYSSSPYVRAPWYQYSEQLLDNVAENGGTHPAFPFLTGMGGTNRVAIFGYLGLGLYYDKLDIDPSLPPQIQYLNYRTFYWQGHGINATSNATHTTLVRLPRENFTLPSANATYFDSPIPVTVGSRSGRNDGSANVTLELRNGEPLVIPNRPIGQTLTVAGNILQCGTILPPPQGNKPGQFPIAAIDGAASTKWQPDFSNVTSYLTVDLGNTTFYPISALLMDWGAQPPSYYEVLFSNSSLPPFSPSQISSNSSFDEVHNVTSGTVQISSPWDPVTAYEIKTYVGNQTNVTLPGVVWSGRYAHLGIRGNQVNGSAGGDGSAGAGAAGARWRSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIK3</accession>
    <name>A0A9W8YIK3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000841</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIK3"/>
    <dbReference type="OrthoDB" id="2830640at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4378011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="372" mass="42533" checksum="0059FE43BE8510E8" modified="2023-11-08" version="1">MIHDPATRSTWAVVFNLPPWTIDTLLQYLGHAKEIAHMPFLVPILLANAASGGLWDREYEAYGHFNTIRIAMKCDQYDRSAIKVDPRDLADMPRKLTASADNVATVVSFAANLERIMKFLDLHFDEYHKHAPPAVYREVQDHLHWLQQLIEDVTVKNETVRSAGQAVVQMVYAVLQQKDNEINHQHGGDMRVIAIVTLMFLPSTFISTWFSASFWNFDPKAEGPVASKWALFHFGISIAMTLAIFIAWRNDSLARKKKHKHMRKSTKSKRKSHQSRWTGKVIRKLRRTPAQVEEGDADTEDDDDDDDYENGRREKDPSPWDSLRQRIVPLAQRRKHSTATQGGEIAKMVRKTAPKKEKAIAEENFAPATKTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIM6</accession>
    <name>A0A9W8YIM6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1760-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIM6"/>
    <dbReference type="OrthoDB" id="5396786at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013877">
        <property type="entry name" value="YAP-bd/ALF4/Glomulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040347">
        <property type="entry name" value="YBP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28020">
        <property type="entry name" value="YAP1-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28020:SF1">
        <property type="entry name" value="YAP1-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08568">
        <property type="entry name" value="Kinetochor_Ybp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="670" mass="74466" checksum="7D497E423BD14AE3" modified="2023-11-08" version="1">MAEPQHQGQARKDEHPLLASRPPVTDPITYLTIVEYNLSEDNLPLLHQVLQDKELTESIGWDLIHLLLPLLPASEECLQDIATKGNPRECVLKVTEALRLLEFEEPHEETDEEEDGAEGSGQRAKVSDVGLGESSTSPAFQQTILPPLPVLKFEILLDLLATLHRRVKTKYPSRFLSTSLQAVLVAYNRANTHIEELTLSAIKFVKTLSGTKRPHLPSRRSSGNLLRTQTNQSEPDPEAQKETPTSEEIELVNRLLQSFLTHILEDYVLSLTSDDDVPGLSWASRLMEKYDPQRIPNKPNYQKYADRFADDEDLTGRAAILGQVVALTQDLGLEYEELLQTVMDAEVEKRGVPGTEDEPPATAEDIPLSRTGSLLLIAARNAKRELYASVKDDESSSIPIFPGHATILSNFIGQLGPQTVGLEPEALLDVVLAIGLIALEKNNIGEPRDDESFAQYLQTMSLISANTPSPSLRGHAHYLTSTILRSHPHDLVRLTFIRDTLEHCPYENLKASAVSWLKGETLEANAPGRHHDDDESETNIFATPVAISTVAPYLWPDLIKQYASAEEADLTDTWIQFRQELGFYVAALNFYYLLLQAEFLHGPLGVKDLEIDEHYLAPLKSTAARFQADLGRGGDKLGIDGDEEALEQARADVGLLEKVVEWVEGGMKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJ01</accession>
    <name>A0A9W8YJ01_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">vps35</name>
        <name evidence="8" type="ORF">N0V83_000225</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in vesicular protein sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the VPS35 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJ01"/>
    <dbReference type="OrthoDB" id="10258141at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030906">
        <property type="term" value="C:retromer, cargo-selective complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.660:FF:000002">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.660">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35, helical subcomplex Vps35-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005378">
        <property type="entry name" value="Vps35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042491">
        <property type="entry name" value="Vps35_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03635">
        <property type="entry name" value="Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009375">
        <property type="entry name" value="Retromer_Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="333"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="362"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="877" mass="99317" checksum="CB3B69E384279FF3" modified="2023-11-08" version="1">MASPPPVEDQARLLEDALAVVRQQTMLMRRCLETPGKLMDALKCSSTLVSELRTSSLGPKQYYELYMAVFDALRHLAVYLRENHPVNHLADLYELVQYAGNIIPRLYLMVTVGTVYMAIEDAPVKEIMKDMMEMSRGVQHPIRGLFLRYYLAGQARDCLPSGDSEGPEGNLQDSISFILTNFVEMNKLWVRLQHQGHSREREQRTKERQELQLLVGSNLVRLSQLVDLENYKKILNPLLEQIVQCRDVLAQEYLLEVVTQVFPDEFHLHTLDQFLSAVARLNPHVNVKAIVVGLMDRLSSYAQREAETESPEQRKKTEEESISQLLEKMRIAKEKKAAEPEPAPAENGDTAETQPGAEEDSAASSTTAVSTADTAVATETEESETDGAIEKRRGIPNNVKLFEIFHEQVQTLVKMQRLPIQDTIGLLVSLANLALNIYPERLDYIDQVLTFANQKVAEYQNSADLHSQATQSQILSLLLSPIKTYISLFTAVALPNFIPLLHSQPYPTRRAVAGEVARSLMRNQTYISSVENLESVLEILKVLIREGIQQATGYPGGPIQRRAQETEETIEEQGWLARIVHLIRGKDNDTQFKLLQAARKSFAEGNERVKYTSPAIITASLKLARQYKKREHFDDNWQSQSSTLYKFMHSTLSTLYTRVTGSADLSLRLFIACGQVADQNGFEEVAYEYFAQAFTIYEEAISDSRAQFQAVCVIASALHTTRNFGKENYDTLITKCALHGSKLLKKPDQCRAVYLASHLWWATEIRALGEEDPKTLYRDGKRVLECLQRALRVADACMDAAVSVELFVEILNRYVYYFDQENDAVTTKYLNGLIELIHSNLQSNENASSLENPRKHFQRTLDYIASREYEGVVTAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJJ2</accession>
    <name>A0A9W8YJJ2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_000272</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJJ2"/>
    <dbReference type="OrthoDB" id="432645at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.790:FF:000007">
        <property type="entry name" value="Mitochondrial ribosomal protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001857">
        <property type="entry name" value="Ribosomal_bL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038657">
        <property type="entry name" value="Ribosomal_bL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680:SF9">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680">
        <property type="entry name" value="RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01245">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="261"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="277"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4377447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="31700" checksum="948533ABE260CBB8" modified="2023-11-08" version="1">MSAVVPCTRAAAPRIVSRTTRCRRLMSTEAAAVQPAHAAPILSRKSEKPARRTLSEQMHRAMYPQLYENEKSKILAPKRPAQAKQQKPIHSKWTQAMHENSVQKGLSEQFRVPVFKPSSVNIKKICPNPIATVTASQSTLLDPTGARTRLFAKDNPECARVGDILLVRQRTGDPFAGVCINIRRRGVDTAILLRGQLTRVGVEMWYKVYSPLVEGIEVVQRAAKRARRARLTYMRTVKHDRGSVENVVRMYLRQKAALGTADGKKKGGAAAAAMGGKKTKGRGKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YKB3</accession>
    <name>A0A9W8YKB3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000906</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YKB3"/>
    <dbReference type="OrthoDB" id="10257415at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021861">
        <property type="entry name" value="THO_THOC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13265:SF0">
        <property type="entry name" value="HPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13265">
        <property type="entry name" value="THO COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11957">
        <property type="entry name" value="efThoc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4378075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="207" mass="23688" checksum="F4C572AF15811CC6" modified="2023-11-08" version="1">MRNKDLVILRLCNELLRRLSRAEDPVFCGRVYVFMFQVFPLGDKSSVNLRGNFHVENVTTFEDFLKDSLKDSGTNADSMQIDDDGSMPTPKEDAKSDEKTPKVVAKDEIRGRKRTTVDTEALYPEFWSLQQSFSNPPRLFEADNFKQFQKTLEATLAKFKEVPKVLQAGDFERNSGGKDGPIDDHDAFANTYNPKYLTSRDLFKLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH57</accession>
    <name>A0A9W9CH57_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tryptophan synthase beta chain-like PALP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_010903</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH57"/>
    <dbReference type="OrthoDB" id="271064at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008721">
        <property type="term" value="F:D-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030378">
        <property type="term" value="F:serine racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018114">
        <property type="term" value="F:threonine racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01562">
        <property type="entry name" value="Thr-dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000005">
        <property type="entry name" value="Threonine dehydratase catabolic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43050">
        <property type="entry name" value="SERINE / THREONINE RACEMASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43050:SF1">
        <property type="entry name" value="SERINE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="6">
        <location>
            <begin position="67"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="378"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="386"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4361962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="410" mass="44091" checksum="F984ADD301B3F7E8" modified="2023-11-08" version="1">MPAETRTPKDTLPLTRESVEAAHKLIQPHIHRTPVLTNTTLSNLASTPQTAEALKGTQWEGQEPAKPKIKLFFKCENFQKIGAFKVRGAFHAVIRLIEEQGIDEVRKRGVVTHSSGNHAQALALAAKTFSIPAHIVMPKISTPSKIAGTQAQGAIVHFSGSTSQEREAVVADVISQTGATLIPPYDDPNIILGQGTLALELQKEASSLLAENAELGVHAAATTQRRLDAVITPCGGGGMLSGTAIALHNSGTRVFGAEPSYQGGDDARRGVASNQRITTVKTLTIADGLRTPLGEHTWKIISDKSYVQGLYAVTEQNIKDAMRLVLERMKCFVEPSAVVGLATVLYNEEFRRMVEREGGVEGWNWGNTTVEAIMKMFGGGGSDGEEERKKEREEGIVGSDGRRVAENVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CH81</accession>
    <name>A0A9W9CH81_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acyl-coenzyme A diphosphatase SCS3</fullName>
            <ecNumber evidence="8">3.6.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">FIT family protein SCS3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SCS3</name>
        <name evidence="8" type="synonym">FIT2B</name>
        <name evidence="11" type="ORF">N0V83_010921</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Fatty acyl-coenzyme A (CoA) diphosphatase that hydrolyzes fatty acyl-CoA to yield acyl-4'-phosphopantetheine and adenosine 3',5'-bisphosphate. Preferentially hydrolyzes unsaturated long-chain acyl-CoA substrates in the endoplasmic reticulum (ER) lumen. This catalytic activity is required for maintaining ER structure and for lipid droplets (LDs) biogenesis, which are lipid storage organelles involved in maintaining lipid and energy homeostasis. May directly bind to diacylglycerol (DAGs) and triacylglycerol, which is also important for LD biogenesis. May support directional budding of nacent LDs from the ER into the cytosol by reducing DAG levels at sites of LD formation. May play a role in the regulation of cell morphology and cytoskeletal organization. Involved in phospholipid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + H2O = S-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:65568"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:156554"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(9Z)-octadecenoyl-CoA + H2O = S-(9Z-octadecenoyl)-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:65564"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:156553"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>an acyl-CoA + H2O = an acyl-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:50044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:132023"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>hexadecanoyl-CoA + H2O = S-hexadecanoyl-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:50032"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:132018"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Endoplasmic reticulum membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the FIT family. Fungal FIT2B/SCS3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CH81"/>
    <dbReference type="OrthoDB" id="5579088at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140042">
        <property type="term" value="P:lipid droplet formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03231">
        <property type="entry name" value="SCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019388">
        <property type="entry name" value="FIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046400">
        <property type="entry name" value="SCS3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23129">
        <property type="entry name" value="ACYL-COENZYME A DIPHOSPHATASE FITM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23129:SF0">
        <property type="entry name" value="ACYL-COENZYME A DIPHOSPHATASE FITM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10261">
        <property type="entry name" value="FIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="294"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4361980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="326" mass="36150" checksum="3FC1170B3FAE6FC4" modified="2023-11-08" version="1">MTTRRKYDPSTEGVPLTAGMPSGTIRPPTSSARRSPFLPTRLEAQLLALYPTTLLLGSIFSTLSPASRSAPYSAVSQSHPPEFAPSYFAQKKNIFNVYFVKVGWFWTTLAFAVFLVFHPGLGKGVSGRRVRAVLRYAAVTTWWVFVTQWFFGPPLIDTMFRFTGGQCELLRDPEAREGMSDTREFITGATCKAVGGTWKGGHDISGHVFLLIMGSSLLWLEFLPALTRVEGLRDGRLITLADGKTASVAVEKERHQTEGDATARGVKFALGVAGLMWWMLLMTAAYFHTWFEKFTGLAVAFFGLWCVYFLPRGVPQIRAWLGMPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIR8</accession>
    <name>A0A9W9CIR8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIR8"/>
    <dbReference type="OrthoDB" id="3440338at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="580" mass="64239" checksum="63430185CBA6B4E4" modified="2023-11-08" version="1">MSIICERALNRSGSPTGAPNSLPEFLYIITNDIVFPSFGSLDGLFTPSQTSHRFTKRKFAWDDLSTKGIETHLENKNRKLEITNFISTYDSEATALKFPPPSVSSNVKGGTQRITKLIIDTQGLVPAWAITTTTTRIPIWIEQGALPKMPKGVLHMTPKGFAAFEATAWICLDEARAILNLKEGVGERGEWMACGYIPRERVCGQVVIDAATKDKTRAINDMVDDASDMKQKAKKKQKREVAVYHGLRAPSIPVRTSSLQSLRPEERRRGDTQGRAIKPPRSRDTKKEHAMKLAQSLLKTALILSGMELEAERSTQEAKETQIRSDDWLPERDPKQQAAITLPGTPMKLTNEHVDGNYDLTNESSSINDRTTNQLLPKNKRLRRIAAQDFASPRETRGSSSREIHPEEPQHDIESMTKDLAATSINTNTPPPSPILPRKQDQRHVTFAINDEDSSSHYSNDVSSLAAESSDDVVVPTDHNLTHRQPTYNDSPPVLATPPPVTQPTDAAPISSVSPLTVAWFERIGHSLDKGIALKTRLAEVTARQQEMQEWREGGNAGYGWASGVLHDRAGLWRGGVGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIS8</accession>
    <name>A0A9W9CIS8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein AF-9 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">YAF9</name>
        <name evidence="6" type="ORF">N0V83_009290</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for an H2A variant leading to transcriptional regulation of selected genes by chromatin remodeling. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histones H4 and H2A. The NuA4 complex is also involved in DNA repair. Yaf9 may also be required for viability in conditions in which the structural integrity of the spindle is compromised.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the SWR1 chromatin-remodeling complex and of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">The coiled-coil domain is required for assembly into the NuA4 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the YAF9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIS8"/>
    <dbReference type="OrthoDB" id="1630758at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1970">
        <property type="entry name" value="YEATS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038704">
        <property type="entry name" value="YEAST_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005033">
        <property type="entry name" value="YEATS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055129">
        <property type="entry name" value="YEATS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23195">
        <property type="entry name" value="YEATS DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03366">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51037">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1 2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="YEATS" evidence="5">
        <location>
            <begin position="81"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="210"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="223"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4364693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="265" mass="30219" checksum="34315D5CB5D71777" modified="2023-11-08" version="1">MAQWADVSSTNRIQHVFLDVNVNDFDPNYVPSFDLETNCPMCRTRTTASSDDALARQLEQKYPTTYAERRAEDEVDRGSRVGEGGVEGVQILIGNKHRLVRGSEDDNQHDWTFFVRTSRPDIVKEVRVDLHPTFRPPRLTLRNPPYEIRRLGWGYFTIEAEIILKEPYSWVLDNTGTRQPGLPLTWTLDFEDRGGQGRVRAKVKRLEANPTNVAAQSLANGPDLEDEDEEEDADYEAAEDDEESSSDDDDDGEDLSEYLATPPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIX7</accession>
    <name>A0A9W9CIX7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein bL17m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mrpl8</name>
        <name evidence="10" type="ORF">N0V83_008687</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the bacterial ribosomal protein bL17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIX7"/>
    <dbReference type="OrthoDB" id="275000at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1030.10:FF:000005">
        <property type="entry name" value="Probable 50S ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.10">
        <property type="entry name" value="Ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01368">
        <property type="entry name" value="Ribosomal_bL17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000456">
        <property type="entry name" value="Ribosomal_bL17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047859">
        <property type="entry name" value="Ribosomal_bL17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036373">
        <property type="entry name" value="Ribosomal_bL17_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00059">
        <property type="entry name" value="L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL17M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413">
        <property type="entry name" value="RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01196">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64263">
        <property type="entry name" value="Prokaryotic ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01167">
        <property type="entry name" value="RIBOSOMAL_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8 10" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="207"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4365070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="244" mass="28542" checksum="E35B57D8501A5174" modified="2023-11-08" version="1">MKYRHLSRSSSHRQALLRNLVTSLFKHESIATTWHKAKEAQRLAEKLVTLGKKNTEATRRRAHQIFYEPNEMVPKLFGPIRERYASRPGGYTRVLRIEPMKEDQAESAILELVDGPRDMRFAMTAKTLARLPPNKTFSPGVATHVKKVTQFREDGVEGLREMVERMRVEQKDGIDNRILPAPRKVYPEERMKKEMHYYEEVDDYKLPNALSKRHPRRKLGKQEVEQDVVIDEQIEGSKPRKQLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ49</accession>
    <name>A0A9W9CJ49_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Cytochrome c oxidase subunit 8, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cytochrome c oxidase polypeptide VIII</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">COX15_2</name>
        <name evidence="12" type="ORF">N0V83_008721</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of a catalytic core of 3 subunits and several supernumerary subunits. The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion inner membrane</location>
            <topology evidence="1 11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome c oxidase VIIc family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ49"/>
    <dbReference type="OrthoDB" id="9974841at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.49.10:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.49.10">
        <property type="entry name" value="Cytochrome c oxidase subunit VIIc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004202">
        <property type="entry name" value="COX7C/Cox8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036636">
        <property type="entry name" value="COX7C/Cox8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13313:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 7C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13313">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT VIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02935">
        <property type="entry name" value="COX7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81427">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIIc (aka VIIIa)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4365103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="79" mass="8938" checksum="DDC1E3F2EF33BB21" modified="2023-11-08" version="1">MLARATFRAASAPSLVARRGFQSTRSQLASPYHYPEGPRSNLPFDPLKRGFAFKYWGFMATGFSLPFLLAVWQTKKNKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJC6</accession>
    <name>A0A9W9CJC6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008339</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJC6"/>
    <dbReference type="OrthoDB" id="63581at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28008">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G10980)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28008:SF1">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G10980)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040976808" evidence="3">
        <location>
            <begin position="23"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="160" mass="17851" checksum="C5AACA57BF5EC303" modified="2023-11-08" version="1">MRIRKPFAAAFIGLILISAAAGFSPPDYTIPTYKQSDKVLHFVAFFLLTIHRDFDYYDIVANVSGSLLALAICNWYHKRMLERKRAARGYAAVAGEEDRDIELGESLGGQESGVVRPTVDEELERWDENAEDWDTTEPEPVNGKALDDGGDLGDGKKRND</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKP3</accession>
    <name>A0A9W9CKP3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Derlin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_007723</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the derlin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKP3"/>
    <dbReference type="OrthoDB" id="19102at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007599">
        <property type="entry name" value="DER1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11009">
        <property type="entry name" value="DER1-LIKE PROTEIN, DERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04511">
        <property type="entry name" value="DER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="179"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4367193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="224" mass="24539" checksum="50F52EC01DB93A76" modified="2023-11-08" version="1">MVFTTKMAPQLWRIFTAFLITKPKFAILLDPYFLYQYGSAIERESSRFSQPGDFFVYTLFVGTVIVTLAGALLNAYTFLPALSLAYAYTFAQDNPTRQVSFFIVNFDSKYLPFALLFMTFIIDGGDAALAQLTGLVAAHLYDFLTRIWPAFGGGKNYIFTPQVVKRWFGATPGSVQNRGYGHVVQGRGRTTGTGAGAEPESAGRSTGVSNAWGSMGPGRRLGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKP9</accession>
    <name>A0A9W9CKP9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">General control protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">GCN4</name>
        <name evidence="4" type="ORF">N0V83_006962</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKP9"/>
    <dbReference type="OrthoDB" id="5419235at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12193">
        <property type="entry name" value="bZIP_GCN4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="3">
        <location>
            <begin position="213"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="152"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="152"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4367380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="261" mass="28428" checksum="33E41844BB48EEA9" modified="2023-11-08" version="1">MTGMVRAYRKSDLELTMFADLNSNNSFDSGANLMPGFQSAGFNMDQVAAFTAINDHSVGTGSIRTVSPKDIFNNAFGSTPPSTAFTNLTSPDIDQSPFISDSFECSPMFQGEPMISNTSSDWFSLFPEEDTKTVETTFAPAPVSLPLERTISSQSMERSGSSSNGSPLVLDSSFRRKSSVTNSPATNGISKSRRRKGPLPAIAVDPSDKIALKRARNTLAARESRQRKFDHVSDLERRNSELEAEIEKWKSIAMAHGYSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKQ2</accession>
    <name>A0A9W9CKQ2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005901</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKQ2"/>
    <dbReference type="OrthoDB" id="41532at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13508">
        <property type="entry name" value="Acetyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="19"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="207" mass="22649" checksum="4F026405E593C854" modified="2023-11-08" version="1">MSDHDPIADAKAQEATHNFVFRNATPEDIPALKKMIEESLRALGKGYYTQAELDGSIGYLFGPDSVLIQDQTYYILHPLNSARTICACGGWSFRQTLYGGDSAPSPLRMPAKRDPAVDRASIRAIFTHPDYSRRGLGTMVMRYCEAAARAGKVGEVGGFARLEMGATLSGVALYERCGYVRSGREDVVRCPNGEGIRVVHMVKNLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLN9</accession>
    <name>A0A9W9CLN9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mannose-6-phosphate isomerase</fullName>
            <ecNumber evidence="5">5.3.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Phosphohexomutase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Phosphomannose isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">N0V83_005859</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-mannose 6-phosphate = D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:12356"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="5.3.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the mannose-6-phosphate isomerase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.8" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLN9"/>
    <dbReference type="OrthoDB" id="6605218at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004476">
        <property type="term" value="F:mannose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07011">
        <property type="entry name" value="cupin_PMI_type_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.441.10">
        <property type="entry name" value="Phosphomannose Isomerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001250">
        <property type="entry name" value="Man6P_Isoase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016305">
        <property type="entry name" value="Mannose-6-P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046456">
        <property type="entry name" value="PMI_typeI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046457">
        <property type="entry name" value="PMI_typeI_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00218">
        <property type="entry name" value="manA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10309">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10309:SF4">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01238">
        <property type="entry name" value="PMI_typeI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20511">
        <property type="entry name" value="PMI_typeI_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001480">
        <property type="entry name" value="Mannose-6-phosphate_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00714">
        <property type="entry name" value="MAN6PISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phosphomannose isomerase type I catalytic" evidence="15">
        <location>
            <begin position="6"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphomannose isomerase type I C-terminal" evidence="14">
        <location>
            <begin position="327"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001480-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF20511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4368777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="407" mass="45233" checksum="180F08875939265A" modified="2023-11-08" version="1">MVEKVLQLKCNCNEYPWGKQGRKSLAATLCEKTPGTDFKLDENTTYAEMWMGTYPDLPSYILATGEDLQDVLDKHPDELLGKRIADKFNHTKLPYLPKVLSIAKALPLQLHPNKDLASKLHAKNPDQFTDPNHKPEIALALGDFEAFCGFKPLKDIERLLQLEPLKHFLPQVKKPAFDDQTLKHVVCAMLKASAENVRKTNDVLTKLPKDAFGKDTYIPDMIPRLSEQYDEADNGTLVALITMNYLQLKEGDSIYIPADGIHAYLSGDIIECMARSNNVLNTGFCPRADRDSVDTFCSVLTFTPHDAKEAILSSQKFDRSKNGKTKLYAPPLSEFSMLATTIGDGESETIGKLGGPTIMIVTEGEGTLKADGKEYKLSAGYIFFVAPSVELDFKATKQIKAFTAFVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLV7</accession>
    <name>A0A9W9CLV7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Thioredoxin-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">txl1</name>
        <name evidence="6" type="ORF">N0V83_005093</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLV7"/>
    <dbReference type="OrthoDB" id="2121326at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.470">
        <property type="entry name" value="PITH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010400">
        <property type="entry name" value="PITH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037047">
        <property type="entry name" value="PITH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46115">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06201">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51532">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="PITH" evidence="5">
        <location>
            <begin position="133"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4370572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="333" mass="35991" checksum="94AD9003536604EC" modified="2023-11-08" version="1">MSGTVEISSPAQLSTLLSSSRVVVVNFYNEGNTSSKAIAPVYDQLASQLARPNRVTFAKVSTVQQAQIAQSYNVTNTPAFIIFKNNQKIRTYSGSNPQQLSEAIKDLAAEAENEGGGSGGFGEAGSSGSGGWRGAALPRGYGDVTDQVDVRGLDLLNSDSDFGGVRTLFESSQPASLKKGKAAATGSEGNKDWVESDVDNQLMLYVPFTSTVKVHTIQITSCVSGDEEDDEAPVRPKTIHIWTNRQHNLGFEEAEDIPAIQAIELKPSDWDEQTATAKLELRFVKFQSVYSLVLFVADGEGDSEKTRIDRIRFIGESGEKREIGKLEKIGADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMK4</accession>
    <name>A0A9W9CMK4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Glucose-6-phosphate 1-epimerase</fullName>
            <ecNumber evidence="3 5">5.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_004540</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the interconversion between the alpha and beta anomers from at least three hexose 6-phosphate sugars (Glc6P, Gal6P, and Man6P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 6-phosphate = beta-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:16249"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58247"/>
            <dbReference type="EC" id="5.1.3.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glucose-6-phosphate 1-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.15" evidence="3 5"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMK4"/>
    <dbReference type="OrthoDB" id="1659429at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047938">
        <property type="term" value="F:glucose-6-phosphate 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09020">
        <property type="entry name" value="D-hex-6-P-epi_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025532">
        <property type="entry name" value="G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122">
        <property type="entry name" value="APOSPORY-ASSOCIATED PROTEIN C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122:SF13">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016020">
        <property type="entry name" value="PHexose_mutarotase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR016020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR016020-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR016020-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4371323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="320" mass="34858" checksum="CCA017ECB591722F" modified="2023-11-08" version="1">MVDRANRPSALNAPSASAPGSQVDIVGQGGSQKVVATLPSGESVEVLLFGATVTSWKSNGGKTENLWVSEKADLTGTKAVRGGIPVVFPVFGPPPKSGHATSSLPQHGFARASRWEFLGKSTVEDALESDSVKLDFGLDRQGLSDEARKAWPLDFGLVYSVTLSKDKLQTVITVRNEGDESFEFQFLLHTYFRIQDISKVAITGLSGTEYIDKVLDATTHTQSDNTLKITGEVDRVYKDIKQDTTSITEDGKPRFDVVRDNVKDTVTWNPWIQKAKAMGDFAPDDGYKNMVCVEIGAVDGWQKLEKGEVWEGGMLVKSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMN9</accession>
    <name>A0A9W9CMN9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable methionine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="2">6.1.1.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MSM1</name>
        <name evidence="12" type="ORF">N0V83_005169</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMN9"/>
    <dbReference type="OrthoDB" id="24670at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.220.10:FF:000001">
        <property type="entry name" value="methionine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023457">
        <property type="entry name" value="Met-tRNA_synth_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43326:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43326">
        <property type="entry name" value="METHIONYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methionyl/Leucyl tRNA synthetase" evidence="11">
        <location>
            <begin position="2"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4370648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="513" mass="58130" checksum="3E71058690217013" modified="2023-11-08" version="1">MVLTDVIKRWHVLKGSKAILCTGTDEHGLKVQRAASKAGVEPKLFCDKGAEIFKDLARKAEIANDHFVRTTDTEHKDAVEYAWFLLQEKGLIYEQKHEGWYSVTDECFYPQSGVQPYLDPPTGRKMMTSIETGSEVEWSSEENYHFRLSAFRDNLLEFYKANPEWITPEHRMKEVVHAVESGLEDLSVSRPSDRLTWGIRVPGDDSQTIYVWLDALMNYATKAGYPWAPGREHAGGWPADCHVIGKDIVRFHCIYWPAFLMALGLPLPKKVLTHAHWTLGGSKMSKSTGRVVDPFHALDRFGSDVMRFYMAHDGGIQDDSSYDNHRIIKIYNNFLNQQLGNLVSRVTRGKKWSVRGAVQRIARKPREDWEEGPGSKFWNNSLSTIAPKIDASFDAYDPRKAALLIADFVRGANAFFQMSQPWSKILDFGPGEPGEEVDKIIYLAAEALRMTGILLQPYMPNKANNLLDQLGVQKDRRTFDYCHPGADLDYGEPMIELGVAHKGTLFPPLSSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMY5</accession>
    <name>A0A9W9CMY5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Piwi-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_004467</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMY5"/>
    <dbReference type="OrthoDB" id="10252740at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02846">
        <property type="entry name" value="PAZ_argonaute_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04657">
        <property type="entry name" value="Piwi_ago-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032472">
        <property type="entry name" value="ArgoL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032474">
        <property type="entry name" value="Argonaute_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045246">
        <property type="entry name" value="Piwi_ago-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16488">
        <property type="entry name" value="ArgoL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16486">
        <property type="entry name" value="ArgoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01163">
        <property type="entry name" value="DUF1785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAZ" evidence="2">
        <location>
            <begin position="344"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="3">
        <location>
            <begin position="667"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4371250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1005" mass="109631" checksum="4B4DC16F2DD43617" modified="2023-11-08" version="1">MADRGRGRGGGGRGEYRGRGGGDRGNFGGGRGRGGGYDGGGDRGRGGFRGGGGGGDFRGGGGGFRGGRGGSEAKVFGNGTFPQPDAEVTKLENAMVKPDLSTALAGSSLDIQLPGRPGYGTAGRAIVLYTNYFELKGIKPDTSIYRYSVAFAPENDLPKARKRRLVEILLTTAPFGGLPVASDWSQILVTPKEIPLGAKRAEYKLEWFPADGDPLPAQSADETDRVKQARRKNTHTALVELIGTVSLKDMLKDLAQPTSTYPLKLETIQALNVIMAHGPSSDRNIATAGGNKFYPFGTHPQLQVADLGSGLQALRGYFSSVRTSVNRILVNVNVATGAFYKSGPLLDVMREWTGGSSPTSDQQYRRMAAFVRKLKIETNYIPESGKAGKAGKAGKTKQTKRKVHVITDLSPFNKNSQNITFGEVDANGKVTKVSVEQYFRTKYNIILASAQAPVVNYGTTKDPKWIPAELCKVLPGQLAKRLLLGPQTSEMIRFAARRPHENAESIMGDGLKVTKINPVANGLNTSLVQFGIKVSPSLLTVPGRILAAPVLQYRAQTTCTPNNGAWNLDTRQLGQRPFRVARKLGSWNTLVINSGNRDTIFGGMQGVTHHLGLFRQTMNTYGLEADPVQAPVAINIQFGDLQNKNLSKIQTEISTALRTKFKAKPNFLFVLLPSDNAVLYDCIKFVCDCQLGVPNICSIGSKFSKEKGQIQYFANVVMKFNQKLGGVNHTVELKKMAPLDDKTILFGIDVTHPSPGSAETAPSIAGVVASVDSLFSQYPASMRTQKGRQEMVAQLDEMIVERLKLWQKRNSGRLPNKVIVYRDGVSEGQYRIVLEEEYPAFKKAFDKLYGAESKHPKISIVMVGKRHHTRFYPTKLEDTDGRTGNPKPGTIVDRGVTGEKLFDFFLLAHQGLQGTSKPAHYVVLRDDNKFGADQLQSLTHNLCYTFARATRSVSICPPAYYADLLCERGRSYLHGVLKGDGAVAFSDSEWHRDVNPALMESMYYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNC7</accession>
    <name>A0A9W9CNC7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_004619</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNC7"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04077">
        <property type="entry name" value="Peptidases_S8_PCSK9_ProteinaseK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034193">
        <property type="entry name" value="PCSK9_ProteinaseK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF58">
        <property type="entry name" value="ALKALINE PROTEASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Inhibitor I9" evidence="9">
        <location>
            <begin position="22"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="8">
        <location>
            <begin position="112"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4371402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="334" mass="34321" checksum="BB8D796CC6B0CB4C" modified="2023-11-08" version="1">MKNGNLEHLVNTALGIIKKDPRHVYNFGGFGGFAADMSDSTLALLRTMPGVEYIEQDAIVKANLGEVQHLEKKAYVTQSSSTWGLARISHVSRGSTSYTYDDSAGANTCSYVIDTGIYTAHTEFGGRATFLANFAGDGSNTDGNGHGTHVAGTIGSNTYGVAKKTKLYAIKVLDASGSGTNSGVIAGMNYSTAVNSAAANVVNAGVFLAVAAGNEAVDAANSSPASEPTVYTVGATDSTDKLASFSNYGTIVDILAPGVSILSTWLNGGTNTISGTSMATPHVTGLAAYILGFEGKRTPAALSSRLTTLSTKNKITGLPSSTKNYLAFNGNPSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNV5</accession>
    <name>A0A9W9CNV5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribonuclease Z</fullName>
            <ecNumber evidence="4">3.1.26.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_003673</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase Z family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNV5"/>
    <dbReference type="OrthoDB" id="527344at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990180">
        <property type="term" value="P:mitochondrial tRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07718">
        <property type="entry name" value="RNaseZ_ELAC1_ELAC2-C-term-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047151">
        <property type="entry name" value="RNZ2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553:SF49">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="853"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="923"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="950"/>
            <end position="961"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4373378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="961" mass="105285" checksum="33B479FABF3988E1" modified="2023-11-08" version="1">MSSQGIRLLKAQDFFLTGRAEWKNTGGLIGMMLTLADSSTSSNAQAWEAHRASLRRGRTSEPPPNPHFNIHGPPNLKHTLTACRRFIFRKGIPINATEYAKKPPQRDEHGAILPTWQDAQIQVWAMATTSAGQDQDSQADAELEAQRQHFDAHLNTFVEHQVPENESIEAREARYDRIRSATIKYMFDSNWSFDTLVERHISEVQMPAAMFVRNPETHKYEAYEGPRPGGALPLPDITVFTRTPWPGAMILALPPTKPAPESVSYIVRTTSSRGTFDVARAKALGVKPGPDFGKLAAGHSVQNQDGDWISPEQVLGADRPGQGVAILDVPSVEYLPSIIQRDELHCPQVMSGIGAIIWNLGPGVAGHTMLKECIGRMSTMQHIISSIDQTPDRIANDSVAGQATRLGQIDPARYSTPVYDNNTVPQRSLYSTSLATSSTLPEKAIAAERGMSFVLMPTFKTKTETATPLFSPDAVRQETAPEVLQLAEKARKAVQDDQAALHAWKQLLARPDTEVITLGTGSALPSKYRNVSATLVRVPGIGNYLFDCGENTLGQLARVFPLEELVDVLKNLRMIWISHLHADHHLGTASVIRAWYHIVHGGVPNSQILDPSAPTIDADAYGLSVISHSGMLQWLHEYSSIEDFGYSRILPLQISPNEHSIWSTLSILNAFGKGENNSSIIDSQELERLTGFSDIQAVRVAHCHGAMAVSITFPPSLADGNNVKPLKVSYSGDCRPSYYFGRIGRDSTVLIHEATFDDELQGDAKAKKHSTTSEALGIGARMNATAVVLTHFSQRYQKIPVLQTVTDGDEQEDPLISPTTIAEDTNEVDDGDVDPTTQNADNMDMHPTITTAKTTTSSTPFKPPTLQHTTSSLHENERVIKVRNKDMKVAIAFDYMRVKIGEIAELEKFNDALNELLTKEDEVGATGSDAVMINANGKKTSGDEAGGGGKKQKKAKSKRNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPB3</accession>
    <name>A0A9W9CPB3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPB3"/>
    <dbReference type="OrthoDB" id="3763456at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="209" mass="23355" checksum="528AFCE6D10A8DB2" modified="2023-11-08" version="1">MDDSQRSGTRSPRDEPPSHSYDLSNAVYGMLPSRATDISRLTSSASSPVRSRSAKRQSTVDAFSISQRSISLGRNSTPPSWLPQTNKRSRMQRTQQMELHQSTCLNLTTASDDSAEADLWILPDFDDWELDTDQAAPVKQQSEWAPLLRRAAKSNLERLRARLEGDGWDFVGGKYEEDKKALQEAASHGEESVDEEFDVVVLPVAQESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPN1</accession>
    <name>A0A9W9CPN1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4360 domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002008</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPN1"/>
    <dbReference type="OrthoDB" id="152248at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025649">
        <property type="entry name" value="DUF4360"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38847">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38847:SF1">
        <property type="entry name" value="PSEUDOURIDINE SYNTHASE RSUA_RLUA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14273">
        <property type="entry name" value="DUF4360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040748597" description="DUF4360 domain containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="24950" checksum="05FC3FE1C4690748" modified="2023-11-08" version="1">MLYKAAAFLLPVLAAATPVPQSGDAPDPNQITIVDTSYSGNGCPQGTVSTSTSPDHTVITYGFDAFQTYIGPGTVPADRSKNCQLHLNLKYPGGFQYAVVDATYHGWARMDDGVTGSFITTYYFSQDAGKTSTTRMTANGGGSLLTGQVYTKQDSVETTATIWSPCGTTGILNINNRISLTSKNTAAAGELSNDDATVAFTQQLHVAWRKCTTTGSGAGSNGDDTIGIGTPSTDVSFGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPX4</accession>
    <name>A0A9W9CPX4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_002858</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPX4"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF38">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="646"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="762"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="785"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="817"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="858"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="945"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="978"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1011"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1040"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1081"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1108"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="653"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="513"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1165"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1165"/>
            <end position="1178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4374117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1190" mass="131115" checksum="DFCB86760EF3F644" modified="2023-11-08" version="1">MRLAEALPHFPDLVSLDLSRTPAAKDDIVLGNLKYLRNLRVLSLKSLGLRDTDVAIVARSIGTRVRSLDMSNNHLTDSSARLLLEHCVKETAIEAHITRGPLPPVQHAHTSDNIDAFETENLVGHLRKKLTEGFVGSLAIEEARDVGITHLYLSRNAVTVEGISGLLRAKRLQVLDIGVLPAILKSPNKTSSGESESDLQLPGVAKLTSVLSEFASAKLKYLRVNYEVITEDAPFEAVPSPRAELSGDLGRYAPSNAHELEAIETPTPELDSMNTEIVELAGNPSYAVELPGSATSDLPLAGSSSDGQGARAPIKALARAPTIEITSEPQVVKRGAAYAPEPVFVDPPLSPVSPLRYSGGNGTPSSSRNSLDVHGCLSPTPPLSDVHVASQLTQFGRRSRNNSTHYVEDRRARLDLRQSQENRLHPGMLPKVHTLVLTDVPAATTDREIVNRLIQYIKDAAEEDSIARQRARHTYILPPGRSRVIAEREYAHSLFALRRIVFEMEPPQAAPKKISTSWREYPTKSSTEDADSEAFWEAATQDFSFFGDEECGLPTHEPGRTLPLAAMSGLELAPQGTAPPPKPRIPEPDFKPILDVVGEIGKFRKEKKAAFENLVRMGVVEPDVEGYWPGDITVVRKRKMFANLKVNAYNLWVVVFVALGTISTAYGLAIIGSTVGQPNFYTYFNLATAGEPGTYPSPPSHSPITHSVPIPNQSHSCNQPPGYDHTTNMIGALNGVNSAGAILGCLFNAWTCERYSRKRSMLLGSAILILGGALCSGAYDMAMFLVGRFVAGWGSGMLACAVPMYQAEVSTPQTRGAMVCTTGVAYALGYSLAGWLGFSCFFLPAESRYAQFAWRFPLAFQCVFPAAVLAGGRFIPFSPRWLLQQDRREEAWEVVRRLHRTSGDPEDGKAREEFKLMEQQYDLDKSLHIDRSFELFRTKPNRRRALVGFIMMWSHQFLGIFVMTNYGVLIYESLGLTGFVPLLLNACWTSFTIVGNVWTACYIDRLGRRTFLLIGSTGCTVSVICLCALTAKFLGTDNIAGLRAAVFFVFFYIFWWCFFVDATQYVYISEIFPNHLRPQGVALGISAFYLGSEVTLVGAPVALNQIGWKFYLVLICPSIIYIGLVWFLFPETKNRTLEEIGALFGDDRIASRWYGLTEEERSRMSHTHDIHGGEKNNEAETTEDISVKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ84</accession>
    <name>A0A9W9CQ84_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transmembrane osmosensor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SHO1</name>
        <name evidence="14" type="ORF">N0V83_001829</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Forms homooligomers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SHO1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ84"/>
    <dbReference type="OrthoDB" id="5983572at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007232">
        <property type="term" value="P:osmosensory signaling pathway via Sho1 osmosensor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11855">
        <property type="entry name" value="SH3_Sho1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000213">
        <property type="entry name" value="High osmolarity signaling protein SHO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035522">
        <property type="entry name" value="Sho1_SH3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="8" id="KW-0346">Stress response</keyword>
    <keyword evidence="6 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="23"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="239"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="192"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4374753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="298" mass="32243" checksum="0D52F7C1F2B6F21C" modified="2023-11-08" version="1">MENGHGYGQRSTFSISRIIGDPFALATLSIGVLAWVIAFISSIISAIHGGFPNFAWWTLVFMFFCIAGVFVTVASDAERTYHVAIVGFLAAGLVFTTSSVNSLVYSPVAPFEAAAAGYILLSMIAIVWIFYYGSQPQASHRAFVDSYALHKEHPGSRSSRPISNGYTNRPETQVNNQAPQMYTSAQLNGFETSSPVSGYPGGPTGANGRNSSAPQFGGMGMNNAATPTHDEPQQEASLEYPYRAKAIYSYEANPDDANEISFQKHDVLEVSDVSGRWWQAKKPNGETGIAPSNYLILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQC0</accession>
    <name>A0A9W9CQC0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Probable beta-glucosidase G</fullName>
            <ecNumber evidence="5">3.2.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Beta-D-glucoside glucohydrolase G</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Cellobiase G</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Gentiobiase G</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">N0V83_001368</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Beta-glucosidases are one of a number of cellulolytic enzymes involved in the degradation of cellulosic biomass. Catalyzes the last step releasing glucose from the inhibitory cellobiose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan metabolism; cellulose degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQC0"/>
    <dbReference type="OrthoDB" id="416222at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.300:FF:000002">
        <property type="entry name" value="Probable beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050288">
        <property type="entry name" value="Cellulose_deg_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715">
        <property type="entry name" value="BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715:SF12">
        <property type="entry name" value="BETA-GLUCOSIDASE G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Fibronectin type III-like" evidence="18">
        <location>
            <begin position="695"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ4376087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="775" mass="82755" checksum="6B789AD14F7356CE" modified="2023-11-08" version="1">MPYFTHSQYETSPPVYPSPNITGAGGWEEALVKARAFVAELTLEEKAGLVTGTPGPCVGNIGPIERLNFSGLCLHDGPLAIRQAVYASVFSAELSAAASWDRSLVRQRGLYMGSEFRGKGAHIALGPVVGPLGRSPFGGRNWEGLSPDAYLSGELVAETVQGIQSAGVQATTKHYILNEQETQRNPSTSADGTTIDAISSNVDDKTMHETYLWPFANAVHAGTSAVMCSYNRINGSYGCQNSKTLNGLLKEELGFQGYVMSDWLAVHTGVASVNAGLDMNMPGGIEFVSGSPSYFGGNITTAVTNGTLPVERVDDMALRIMTPYFFLNQDADFPPVDGYTPKLGFFPQANYVYNFTLGPVVDVREKEHAKLIRDLGAAGTVLLKNVNSTLPLKQPMNIGVFGNDAADFTQGQYSLTLVGTGLANGDYDIGTLAVGGGSGTGRFTYVVPPLDAIKSRAQSYGALVQYITNNDVIANGGFSSLAPLPLDVCVVFLKSWATEGFDRTTLIAEWNSTVVVEATAAICPNTIVVLHGSSPNVLPWADNPNVTAILAAHMPGQESGNSIVDILWGDVNPSGRLPYTIALNETDYAKNLVNSTELLETTDPNAWQADFVEGNLIDYKEFDAKNASVAYEFGFGLSYTTFEISDLKVDIAVTNASRTPDPEAQIIPGGNVELWATLATVSVAITNTGDVAGSAVPQLYLSYPDEAQMPVKSLRGFEKTSLAAGASCVVQFPLMRRDLSYWDTTAQAWRLPTGDISVSVGWSSRDLPLQGVLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR35</accession>
    <name>A0A9W9CR35_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_001308</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the paxM FAD-dependent monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR35"/>
    <dbReference type="OrthoDB" id="9993796at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000331">
        <property type="entry name" value="FAD/NAD(P)-binding domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050493">
        <property type="entry name" value="FAD-dep_Monooxygenase_BioMet"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13789">
        <property type="entry name" value="MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13789:SF187">
        <property type="entry name" value="MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="6">
        <location>
            <begin position="17"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="7">
        <location>
            <begin position="122"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4376028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="436" mass="48584" checksum="49DC641EB70F86B5" modified="2023-11-08" version="1">MTASNTNGTTPKTGIKVIIIGAGFGGLTAAIECIRQGHTPVIYEAFPSLKILGDIISFGPNGGRIFARWSNGSVAQKMRGVSIDLIQHGFNIHKYDTGEVVINQKTPPRDETAPNFNGHRGELHEIVFEYAKEIGVEINLGHRVETYWEDERAAGVVLEDGTKVEGDLVIASDGVRSKARTLVLGYEDKPKSSGYAVWRAWFSNKDMIADPETKHFCDNGDTFNGWIGPDVHFLFSTVKNGTDCCWVLTHRDEHDIAESWSFPGYLKDVKEVLEGWDPMCWKIISKTPEEKLVDWKLVYRDPLPNWVSGYGSAPGHGRICLLGDAAHPFLPTSAQGATQALEDGVTLAILLRKAGRDNIRGALRAFQDIRYDRVKAVQKTGETTRDMWHKTDWEKVKKDPTLIQFPREDWVFQFDAEKHTEEAGDEAIKKASATVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRB5</accession>
    <name>A0A9W9CRB5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Exo-1,3-beta-glucanase</fullName>
            <ecNumber evidence="2">3.2.1.58</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">EXG1</name>
        <name evidence="2" type="ORF">N0V83_000655</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.58" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRB5"/>
    <dbReference type="OrthoDB" id="1046782at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23668">
        <property type="entry name" value="GH55_beta13glucanase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039279">
        <property type="entry name" value="QRT3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024535">
        <property type="entry name" value="RHGA/B-epi-like_pectate_lyase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33928:SF2">
        <property type="entry name" value="PECTATE LYASE SUPERFAMILY PROTEIN DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33928">
        <property type="entry name" value="POLYGALACTURONASE QRT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12708">
        <property type="entry name" value="Pect-lyase_RHGA_epim"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhamnogalacturonase A/B/Epimerase-like pectate lyase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Rhamnogalacturonase A/B/Epimerase-like pectate lyase" evidence="1">
        <location>
            <begin position="289"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="567" mass="60291" checksum="4E0F8AA943817EEC" modified="2023-11-08" version="1">MTQLIGNPKSLPVLKAAPTLDALALIDASPYSNQNGAPGWISTNLFMRQIRNLVIDGTAVAPTKGFQGIHWPASQATTIQNVKIRMTQSSDSVHAGIFVENGSGGHMADLEIEGGEYGMNIGNQQFTMRNIKISKAVIGISQIWNWGWLYSGLSISDCGTAFSMINGYTSNKLEVGSVVIIDSEITNCPRFVDMAWKTNSVPIGAGQLTLENIKLTNVPIAVMGSGATILAGGTLTIQAWGQGNKYSPNGPVKFQGPITAASRPAGLLDGGKYYSKSKPQYETLTVSNFVSARGSGATGDGSTDDTKAVQNAINTAVSQNKVLFFEHGVYKVTQTIYVPPGARMVGETFSVIMGSGSTWSNMANPAPIVQIGKSGESGSIEWSDMIVQTQGATPGAVVIEYNLNSARGSGLWDVHTRIGGAKAQNVFAGFIQTETPYYMPSPDAKTQPYGRSDTYSDPNYATACTSGICDAYGLRVLNSKSILIYGGGLYSFFKNYDVSCSSPDAANGFRNCQTRIFSIEGASSVQAFALSEVGAEQMLTIDGVDKAKWSDNLSVYSNTIGYLAYNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRJ0</accession>
    <name>A0A9W9CRJ0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nuclear actin-protein involved in chromatin remodeling</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ARP5_1</name>
        <name evidence="2" type="ORF">N0V83_001748</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRJ0"/>
    <dbReference type="OrthoDB" id="7340501at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000139">
        <property type="entry name" value="Chromatin remodeling complex subunit (Arp5)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="166" mass="18306" checksum="63D2BA0783F22D26" modified="2023-11-08" version="1">MAPSAINDTPLASGRNSPKPPPAKLWSVKEPPFEGYRRADTEGWARSNHETAIVIDNGSSAVRAGWSFDSKPRLSVLPNMARYRDRKLNRTFTFIGSDVYSDGTARGQSKPVYEPGSNIVNNWDAMEGVLDYCFIKLGVDGKNGRIDRPIVMTEPIANLGYSRKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CS00</accession>
    <name>A0A9W9CS00_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein phosphatase regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SHP1</name>
        <name evidence="5" type="ORF">N0V83_000429</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CS00"/>
    <dbReference type="OrthoDB" id="25887at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061025">
        <property type="term" value="P:membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031468">
        <property type="term" value="P:nuclear membrane reassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14348">
        <property type="entry name" value="UBA_p47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01770">
        <property type="entry name" value="UBX_UBXN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000179">
        <property type="entry name" value="Plant UBX domain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.210:FF:000002">
        <property type="entry name" value="UBX domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.210">
        <property type="entry name" value="SEP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036241">
        <property type="entry name" value="NSFL1C_SEP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012989">
        <property type="entry name" value="SEP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23333:SF20">
        <property type="entry name" value="NSFL1 COFACTOR P47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23333">
        <property type="entry name" value="UBX DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08059">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00553">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102848">
        <property type="entry name" value="NSFL1 (p97 ATPase) cofactor p47, SEP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51399">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SEP" evidence="4">
        <location>
            <begin position="231"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="UBX" evidence="3">
        <location>
            <begin position="351"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="43"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="289"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="57"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="163"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="313"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4377602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="432" mass="45850" checksum="C59ACA61914B6099" modified="2023-11-08" version="1">MSEGAPENRDAQIAQLASLTGLDPTQAERYLSAANGDLTLAATLYFDESTEQPDPPEPLDEEMAGDDQTPDPNPPQQPVPGGGRTLGGAYVPPAEGSSSSASKPSGTSRQAPQRGGLRTLKDLQSSGGGHGHGHAHDDGDSDDDEQDGENQDFFAGGEKSGLAVQNPNQANPRDQINNILKRARQNAPRPGGDDERPSSHFRGAGTTLGGDDAPSRTIPDPTANIPAPPERAHRELHLWRDGFSVDDGPLFRYDDPANARTLEMINTGHAPLHILNVEHGQEVDVEVHAHKDEDYKQPKKKYVPFSGSGNRLGSPTPGASSAAAPPTAATSSTTTASTSTGSAQPTVNVDSSAPTVTLQIRLGDGTRLQSRFNTTHTIGDVYDFVTRASPASQEREYALMTTFPSKELTDKAQTLGDMAEFKRGGVVVQKWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XX94</accession>
    <name>A0A9W8XX94_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_010984</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XX94"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053210">
        <property type="entry name" value="ANKRD12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056485">
        <property type="entry name" value="ARM_KRIT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056015">
        <property type="entry name" value="DUF7593"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24149:SF14">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24149">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24521">
        <property type="entry name" value="Ank_KRIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24513">
        <property type="entry name" value="DUF7593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="346"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="379"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="KRIT1 ARM-repeats" evidence="5">
        <location>
            <begin position="457"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7593" evidence="4">
        <location>
            <begin position="916"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="593"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="421"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="171"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="193"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="597"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="623"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="658"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="676"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="698"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="714"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="797"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="818"/>
            <end position="914"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4362042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1134" mass="126245" checksum="C12AC072870F4A96" modified="2023-11-08" version="1">MTSNADSVLNASSLPAAQHDDDRPRSPPSPRQPAPKAASPSSKENHPLPPNGHDGHDEDAEGESEAETVVLDARGDSSPAKKPIKTERIDDDDGAEPLRSIKEKSHAHSPQSNGRRRSSNGKVVKINGVKNGSERDAKSPVPTSPSSLTTSRHTKDTSPSDSVKSPAPSALSPTQTTTRGRSASTVESRKRKLRDDSFAKSIEPPRQKAKTEGLKESRQPNSPATPGFGRSHKRSQSTQSVVTGAAGRKRRDVTALALSTENTRWSESSSEHSTSPQPTAAPHAQRDRVKRSSHRALTSPARTMPQRKTDRFGATRLAREAEKGDLDAVKEAFEDASDELDSVDYAGIAPLQKAALHGWGDVVEFLISKGCRTDCESNDRDTPLIDAVENSHLEVVKLLLNQGHVNPHHQNKKGQRAIDVLDYEDEDAEEIEKELKEAMKREVDTSATNEHEQSGRKQAKTASRLLYNEYNVETLIEKAGDGDISAVGELINSNIKPNITCGVAAARGGHYDILSILLASGLKADPDPSKHPETPMTVAIGRGHLRIIELILEQDNFDPTRRNKEGKTYYEISEDRGGPKWEQERDILKRAYDEHKGLHRSPRRVKKEAPNPSAVRMKRKSSPRRERSSSPRHEAKRAHLTKSTTVPAPPQKPRRLLSGKEKANREGQRRKRVVDDESSEEESEEDVRPPTRKAKPRSYSEGEEAKPVRKALKARSDDNETKRPARARSDESDEDRKTEQAKISAKPVKSQNAKPTPEVDSPDERKPLRTKIKYKTKDDKLRKRKFSDASADDVSVKKTPTASAKSTPAPPERTATPEVERARRQEELRQQAEAKRKAAEAAEAAAKAEIRKKEEEEAVRKAAEEEEARKKAEAEAEAQRQAEEAEAARKREEEETQRRAAEEAERQKELAARQDRISKLPRALRRACELGNNRPLHFSGEELGVSLVFLPLLYATAQDLRNSEALPGKTYICSFQLVGILGLPELDLAHLDSPYSNWPRIPVTRKQRDVILRHYDVALLAQDFRFPMEGTPDFDFGKIQESIKEAKNQFLAMDGLYWIEESHLRAEVENKESLRPLLDDLKPECRTKRIYLADLGSEEKTEKTHRPRKSFMDLVLEQNGVNGVAAHATVNGNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXA0</accession>
    <name>A0A9W8XXA0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Altered inheritance of mitochondria protein 18 mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">AIM18</name>
        <name evidence="3" type="ORF">N0V83_010824</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXA0"/>
    <dbReference type="OrthoDB" id="18193at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016872">
        <property type="term" value="F:intramolecular lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016087">
        <property type="entry name" value="Chalcone_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016088">
        <property type="entry name" value="Chalcone_isomerase_3-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036298">
        <property type="entry name" value="Chalcone_isomerase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47284">
        <property type="entry name" value="FATTY-ACID-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47284:SF3">
        <property type="entry name" value="FATTY-ACID-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16035">
        <property type="entry name" value="Chalcone_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54626">
        <property type="entry name" value="Chalcone isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Chalcone isomerase" evidence="2">
        <location>
            <begin position="207"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4361883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="461" mass="50722" checksum="9ED827D5928A107C" modified="2023-11-08" version="1">MPIRLAARRPIFPCLNAPTAPLRSNPQLRHKSRYARSDLEAVLRQPTKPKGPRLGEDFVSVHREEALRRAAATRRRNWLALGAVLSMAAPILIVKWMGLPLDGIDAKKDENKKEENQGRGVVEMITGPPTKTDAPRKAADEFQGKKIVIAAGDKVIAAPASSENPQASDADAIELVETGTSYVPYFPRTIRLPTDTSGPEQITSDAEYTLLGLGIRKVSFLRVQVYVVGLYVKTADLSTLQNHLINTVNPTASALIPGEKEDLRQSLLDSEKSSKIWEAILSKEGPVAVDMAFRVVPCRGTDFKHLQDGWMRGIASRTDEVRRKQAELLRQQAAESQSIALPKPVEENEFSDESFGLAMKEFKNLFQGKGKAPKGSVIILARNKSGSLGAMYQPVVQKDGKERMGKSNWLGEVKDERVGRLVWLLYLGGQNVSSEDARKNIVNGCISIVERPVGTVEGMVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYE2</accession>
    <name>A0A9W8XYE2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase</fullName>
            <ecNumber evidence="5">2.7.8.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">GlcNAc-1-P transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">N-acetylglucosamine-1-phosphate transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ALG7</name>
        <name evidence="20" type="ORF">N0V83_010149</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes the initial step of dolichol-linked oligosaccharide biosynthesis, transfering GlcNAc-1-P from cytosolic UDP-GlcNAc onto the carrier lipid dolichyl phosphate (P-dolichol), yielding GlcNAc-P-P-dolichol embedded in the cytoplasmic leaflet of the endoplasmic reticulum membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-N-acetyl-alpha-D-glucosamine = an N-acetyl-alpha-D-glucosaminyl-diphospho-di-trans,poly-cis-dolichol + UMP</text>
            <dbReference type="Rhea" id="RHEA:13289"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19507"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58427"/>
            <dbReference type="EC" id="2.7.8.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:13290"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.15" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYE2"/>
    <dbReference type="OrthoDB" id="10262326at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003975">
        <property type="term" value="F:UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06855">
        <property type="entry name" value="GT_GPT_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033895">
        <property type="entry name" value="GPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10571">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--DOLICHYL-PHOSPHATE N-ACETYLGLUCOSAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10571:SF0">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--DOLICHYL-PHOSPHATE N-ACETYLGLUCOSAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="351"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00045078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ4363031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="383" mass="43266" checksum="ACEA2613E272E870" modified="2023-11-08" version="1">MEIPETMGAVCAMVYLFTLIVFIPWPFYKDIVVATSGGGNRDVIKELEEIETGRLLHRFPHNKLASYLSAILSLQSIVLLGIGDDLFDIRWRHKVLIPAFAVIPMLVVYFVDFGVTQMVVPVPLRPYLGELFDLSWLYYIYMALMSIFSSNSINILAGINGIEVAQSVVIAVLIVGNDVLYLSPFTPYPHPATDSHLFSLYFLLPFIGVSTALLMHNWYPAKVFVGDTYCYFAGMVFAVVAILGHFSKTLILLLLPQAFNFVYSAPQLFHLVPCPRHRLPHFNARTGLLEPSRVEFRKPLHRPIAESLKLLHRLRLLDVEVDAKGQVVSSSNLTLINLWLVWFGPMREDRLAMGLLAFQFLIGALGLFIRHRMALLIFTADNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYZ9</accession>
    <name>A0A9W8XYZ9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYZ9"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF31">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="334" mass="36784" checksum="557D213EC556E29F" modified="2023-11-08" version="1">METIKKSIVGTFDPNNDIPNLSGKIYVVTGGSAGIGYGIVAHILQHNPAKIYLLSNKEDHATEAKDALKEWGDVNKVEWRQCNLQSFKQTDEVAKQLAQELTRLDALVCNAGLGVGVYNETEDGLDSHMQVNVFSQAHLTLTLLPILQKTQDSRIVHQSSDLHKGAKSDMKFESIAEMNQDIGAMLLYNRTKLAQILFLRELYFRIKAGHFGSVTGTQGLPWINATHPGGVVTDQQDQAVEAYGTLGKIGVAAVRPFMKDPVAKGCRPALFAATSPDIAKDEIQNSYIVPDRKVQEPSNQAKEGALALNLWKLTKEVLESKIRDLPYTMKTDVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZG6</accession>
    <name>A0A9W8XZG6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF5648 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZG6"/>
    <dbReference type="OrthoDB" id="9971254at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043708">
        <property type="entry name" value="DUF5648"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18885">
        <property type="entry name" value="DUF5648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5648" evidence="1">
        <location>
            <begin position="3"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="351" mass="39147" checksum="1758FD14AFB97D9D" modified="2023-11-08" version="1">MSHHFYTTDATGELAPGVGYQYDGRFVDGARLDGTSGYYVFTQAQEDTVPVYRFFNPSINDHFYTTERDGIAEKIGYDFEKIAWHMPSEQGPDNTAYFRFYDPDTKDHLLTTDYLGEPGYNNDGVLGYIYNYAKPGATELQGFVHTGYTLVGTPASQGTTYTLTTTMCDMASRTNKTSQLPLKYVVGRTGTNIEICVQKAPSDADIKKGSVSLAELDGDGRGYLLLHKPSTLHLRYIGIGFLDIDDPSDSECDVGPYIRRGINGLIEFVHPNPVNPSTQKESTPQWMTDVLPIIDRALQSGDDVMVTDLMWAITEYWKAMYFDIPGPSVTDFLKGTYLEDGKLIRPGIWVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZX8</accession>
    <name>A0A9W8XZX8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF1593-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009647</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZX8"/>
    <dbReference type="OrthoDB" id="3592035at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="entry name" value="Ribonucleoside hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048527">
        <property type="entry name" value="Sde182_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011483">
        <property type="entry name" value="Sde182_NH-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21027">
        <property type="entry name" value="Sde0182_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07632">
        <property type="entry name" value="Sde182_NH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cellulose-binding Sde182 nucleoside hydrolase-like" evidence="1">
        <location>
            <begin position="2"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Cellulose-binding Sde182 C-terminal" evidence="2">
        <location>
            <begin position="271"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="395" mass="43918" checksum="9C606293CA717DEF" modified="2023-11-08" version="1">MPTYGMSAVGTDIPLSTGGQLLYDRIVAPSSQPLWILCWGGTNTLAQALLKIDDKYPPEDSKRLLSRVRVYAISDQDDTGAWIRNNFPDIFYIASVHGWNQYGMAAWTGISGERYYNFDQGGPDFTKMEKSWIKENIQIGPLGSAYPDYLFIPEGDTPTFLYLIQNGLGVPECPEFGSWGGRYARTDVSGEGLNSNHYSDAVDHVKVGDRTYTSNHATIWRWRDAFQNDFAARIKWSMEPDFSKANHHPVVVVNDAKGLAPVRIDAEAGSTVTLDATGTYDPDGGDLVFKWWHYREPTATQWWVSAEVSELAVKNVDAEGRKVEVTLPPPEKCAVELMSRKPMEKGQLLHLILEVTDSGSPSLTSYRRVLIQVTNKELRGGGEGADAIGDLMKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZZ6</accession>
    <name>A0A9W8XZZ6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Vacuolar calcium ion transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_010044</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Has a role in promoting intracellular calcium ion sequestration via the exchange of calcium ions for hydrogen ions across the vacuolar membrane. Involved also in manganese ion homeostasis via its uptake into the vacuole.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Vacuole membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZZ6"/>
    <dbReference type="OrthoDB" id="1699231at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015369">
        <property type="term" value="F:calcium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004713">
        <property type="entry name" value="CaH_exchang"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004798">
        <property type="entry name" value="CAX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00378">
        <property type="entry name" value="cax"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503:SF14">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0050">Antiport</keyword>
    <keyword evidence="6 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 10" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="387"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="418"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="12">
        <location>
            <begin position="120"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="12">
        <location>
            <begin position="323"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="15"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="59"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4362927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="483" mass="53213" checksum="2A605646CBB0ED68" modified="2023-11-08" version="1">MARSRTSREYPNGRSSRDQTNGRRSREYTTEKTSRTDPSHVSTDRRDRRRSSLPQYNHNGHEVTKHIHPEGESGRRGFHPFQFLAICGRSSSTTSMLVNLLWPVVPVAIALHFARPEWHIPIFITNYIAMVPAANMLGFAGQELSRKMPQKAFAVVLETTFGSIVEIILFMTLLKTSVNDSNVSVIKAAILGSILANILLCLGTCFVAGGLKNDTQEFHSAVSENGSGLMLVAAMALVLPAVFYQYLYNNTDYITTSTMITDRTIKISRGVAIISIIGYLIYVAYQTISHDGLFHEIYEADDHKDKDRHEELAKPKLTLTEVVVALLISLACVSLIAIFLVLEIPHLVERGVSDSFIGLILIPLVEKLAEHLLAIDEAYDNQINMALAHVLGASIQTAMFNAPLVVLVGWGLGVGMDYNFAIFDAVALVLAVIAVGSFLRDRKSNYLEGVLCVMTYVIIAICAFYFPNPAEHGTGEAAEGSQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y002</accession>
    <name>A0A9W8Y002_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y002"/>
    <dbReference type="OrthoDB" id="3800937at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="361" mass="39741" checksum="7A6BEBDDF86B83D8" modified="2023-11-08" version="1">MVYVLNTDNLSQAEVQRLQAQWKPVFNPQSQEPEPRSDVPVRGRGQVTYRAPPGPAAGSDGRDTRGNQSSSLKKRKTRDDEDTQGPGSQVPLERSPALANVPLIKQSDEARRAARAIRFAPGSTATARSFNNVPSKVPDKHTTSDLGKDHDKSPLVNTATARSNVPEKVPEKHTPSSNLGKRSRVPVTPAKIFKVKPDPIVEPNYEYKAPFFDYGNDSLPILYSIWINHVDGKQPITEPSFALRQDALALYRRSWSGDDLGRTDTPKTGPTRTIDDVDLYFRHGKIHVAAERGLLLAADYLRMIGVPDTQPVRFDGKKPAWAKEAAARAAVGKRDYRGADKGLIDWATFSYAGLLAKQGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0D0</accession>
    <name>A0A9W8Y0D0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0D0"/>
    <dbReference type="OrthoDB" id="3790813at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="317" mass="35734" checksum="CC4B9EC9BB558D38" modified="2023-11-08" version="1">MSFFSRRPSHRPSTPPPDFATTSPPACEYDTDDTSLSWDLLTPPRTPLLTPLTNSNSSSSSSTFFDTETFPAQSYLFPSEQNGTRRTNALHALHHPVETATSLRRHASDLNWSTCRTTLLDAADSASFHLNECLNQVFEGSDVVEMHNRVASLLPLVSLPNMRQLSQTIDSVSDTVQQSIAVPLPPRHHHHRCLRESPAFIERELQGDQWAFARIKSAEARHVLVKRGICREWQVVHNQLTASWQNELNMALAATFTREKEQYGVVALFEMTPVWVLDGARKAWRLELDTGLADIVVEGPEMYGFREVEVRLGWALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0I7</accession>
    <name>A0A9W8Y0I7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_009134</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0I7"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047499">
        <property type="term" value="F:calcium-independent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185:SF1">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="5">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="4">
        <location>
            <begin position="26"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="28"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4364539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="515" mass="58720" checksum="0480EE866E8D1761" modified="2023-11-08" version="1">MKELGNKLNPKRPEIKPCEYFDLIGGTSTGGIIAIMLGRLRMSVQECIDEYTLLSRRIFQRKRGKGSSGPQFYAEDLEDEIRKVLRRRLGPHNEDAPLRDPLDEEACKTFVLSLQDQNARAAPQRLRTYPSADEISCTIWQAARATSAAKTFFNSITFGNPPIEWVDAGLGYNNPAREVLDEAQLLWQDESGVFNFKDQIGIFISLGTGIPDVFRPDKAKSMTKKVVDDLANRFGVPPDVIRQMKDIVLNTERVHDELFPSFRSQGCKEIYHRYNVARLGDIDLGDYEKEEIMTVDTNAYLHEQRFNIIDCANLMKRLPVYVLPMEGTIEEPVQDEQAENAQAQIVDTDGIDLEAQKTVVWKDLVRGSFREHRARQSLTDFHLSDMGRKAKETLKNVAVSKHVEVQLRANVDLWSAYKLYRKVRIFAKAQLAVLSHDSANASTAQDAGQSLASIHAWYYWITHRLAGVSVSLGMLQSALSLYQEAWRGRSDMHPRIERDIQKSYKGMEKMREFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0M2</accession>
    <name>A0A9W8Y0M2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="3">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_009687</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0M2"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="6">
        <location>
            <begin position="77"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="562"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4363394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="603" mass="66988" checksum="EE2155823C349615" modified="2023-11-08" version="1">MVDARSASPESDGDRIQKRTFSARWLKRRLTVIMIFFGTTLLLIWYFGAFGLGRPYPQPPYEPTQPNGSLVIDLPKYGSFRGTQVISNLRKTKKFSSPVDAWMGIEYSTQPADKERFKAVTWPEPFTGTRNATSNGPACFQNMYGSLGQSEACLTVSIFRPSGIPMDQKLPVLAYLHGGSFVVGSHRSFDGAMFVQKSAQPLMVVTAQYRLGALGSLPGDFMEREGLLNLGIRDQKMMLRFLQRYIENFGGDPQKITLGGQSAGGHSVGIHLFHDYGNDKGKPLFHQAILASGSPTARAFPGVDYALYKKQVDHFMDYVNCPTTPASQALECLRSAEADDIQFISSSLYNANNHNITWPWQPVSPGPLFQKRGSTSGEDGTFFKIPMLISSTTNEGKAFVPHDIDTNNDFLGFWKTLVPGLTPQDLTDLDEMYPDPDTVEDSPYKTEVGTFISAQYERISAAYGDYSYICPVQDTASRLSSADAPVYKARWNTPNHAPTYMGVPHASDASYFNGQVGTQYPEIADLYSAYWASFVVSGDPNTHAQEGAVTWEKYDVKDGKSGRELVVSPPTKGGAQMEDEDKGIRMDQCEWWRDEDRAKRLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0T9</accession>
    <name>A0A9W8Y0T9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein transport protein Sec61 subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_009752</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC61-beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0T9"/>
    <dbReference type="OrthoDB" id="5401193at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005784">
        <property type="term" value="C:Sec61 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030671">
        <property type="entry name" value="Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13509">
        <property type="entry name" value="SEC61 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="501"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="137"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="171"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="187"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="290"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="358"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="407"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="457"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4363459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="56043" checksum="BAF7E6403D8A466E" modified="2023-11-08" version="1">MSYYNDPSWPSPAPGRQSSWEQPQPPSRSGTGHSAYNGGGAPIHMDIGASSTVNRDEASAFSSQFDEVERATENLAKSGKSFGPGFPPTPVSGNRRESMPMMGGGGPPRQYPDYEQRMGGPGPQRHHSVSEYDGSRSHSASNVQGFYQNQRYAPRPNDADQMAQAKRRMAAQRERELRNYHQEQQYHRNVSGSKSDRSMSPSAMNEDERRELIARQHRALYGDGSTLYNNNPTSSQDVRVQTSGAGRGNSPLAFDPFGMQAQNPSGDAAVQMPPRGDKDSAGGAQEARANSNQSPSSNQNPAFSLFDAQQASRTSNSSPGGSPPISGPKSNGSGVAPIGTRPQNQNLNQPAGAPLGKRTTSPLPSPLGYNSYNASEQKQNNAATTSASLNPSSTVTDKGVGIWNNGPWGNTPNQGVQASSTPRPTSPVNTGSSGSTTARPASPKPPGGPATVMRRKAAADRAEKTANQRPSSTRAAGAGGSSSTMLRLYTDESPGLKVDPVVVMTLSVVFIFSVVALHVIAKVMRRFSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0Y6</accession>
    <name>A0A9W8Y0Y6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_009636</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0Y6"/>
    <dbReference type="OrthoDB" id="423462at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089701">
        <property type="term" value="C:U2AF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12538">
        <property type="entry name" value="RRM_U2AF35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000066">
        <property type="entry name" value="Splicing factor u2af 23 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009145">
        <property type="entry name" value="U2AF_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12620">
        <property type="entry name" value="U2 SNRNP AUXILIARY FACTOR, SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01848">
        <property type="entry name" value="U2AUXFACTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="9">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="44"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4364180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="24231" checksum="BD9D844B984159AA" modified="2023-11-08" version="1">MANYLASIFGTEQDKVNCSFYYKIGACRHGDRCSRKHVKPSYSQTILLPNLYQNPAYDPKNKMNAQQMQMHFDAFYEDIWCELCQFGLVEELVVCDNNNDHLIGNVYVRFKYEEDSQKACDALNSRWYAGRPIYCELSPVTDFREACCRLNSGEGCVRGGFCNFIHRKEPSAELDRELDMCTRKWLKERGRDARSMSKSPTPPPAAKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1A9</accession>
    <name>A0A9W8Y1A9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycoside hydrolase family 76 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009366</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1A9"/>
    <dbReference type="OrthoDB" id="4104179at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005198">
        <property type="entry name" value="Glyco_hydro_76"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053169">
        <property type="entry name" value="MUG_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47791:SF2">
        <property type="entry name" value="ENDO MANNANASE, GH76 FAMILY (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47791">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 191 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03663">
        <property type="entry name" value="Glyco_hydro_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="291"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="47901" checksum="2E640E2D719B87DC" modified="2023-11-08" version="1">MPGTFDKSRKLDVEKQMAENEINKYFAQITSYYFGEDHFAIRLQAFDDMLWVVLGWIEAANFVSVHSETHYSPAGTGEFEEEQPGWRGSKEWHARQFIPAFAHRARVFYELAETGWDWRLCGGGMTWNPRLLPYKNAITNQLFISASIAMYLHFPGDENCSPFLATKSYDDDGDGDDDCDSNFRGRYDPMYRANAIIGYEWLKNSGMTNEQGLYVDGFHISGYRQNHSKTTCDERNEMVYTYNQGVILSGLRGLWEATGNVTYLEDGHELIRNVIDATGWKEMDVHSTSRQFTAQQTQKSSSKSGEYITKDHEDASPSSSSNILGTSGILTELCDPSGTCSQNGQTFKGIFFHHLTSFCSPLPTRRPTAPGRTYAAPRELATLHHRSCKEYAAWVVHNAQAALRTRDEEGGLAVGGGAWERGEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-06-18" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1G4</accession>
    <name>A0A9W8Y1G4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Midasin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">MDN1</name>
        <name evidence="13" type="ORF">N0V83_009765</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Nuclear chaperone required for maturation and nuclear export of pre-60S ribosome subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the midasin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5186at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000142">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000582">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000712">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001053">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001368">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040848">
        <property type="entry name" value="AAA_lid_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048617">
        <property type="entry name" value="MDN1_AAA_lid_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012099">
        <property type="entry name" value="Midasin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041190">
        <property type="entry name" value="Midasin_AAA_lid_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103:SF2">
        <property type="entry name" value="MIDASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103">
        <property type="entry name" value="MIDASIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17865">
        <property type="entry name" value="AAA_lid_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17867">
        <property type="entry name" value="AAA_lid_7"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21108">
        <property type="entry name" value="MDN1_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010340">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="12">
        <location>
            <begin position="4662"/>
            <end position="4802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="712"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="4015"/>
            <end position="4404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="4420"/>
            <end position="4529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="4811"/>
            <end position="4840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="4020"/>
            <end position="4033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="4051"/>
            <end position="4060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="4061"/>
            <end position="4071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="4072"/>
            <end position="4095"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="4103"/>
            <end position="4112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="4126"/>
            <end position="4149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="4167"/>
            <end position="4195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="4201"/>
            <end position="4212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="4213"/>
            <end position="4254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="4261"/>
            <end position="4276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="4277"/>
            <end position="4291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="4292"/>
            <end position="4314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="4315"/>
            <end position="4324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="4340"/>
            <end position="4349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="4387"/>
            <end position="4404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="4420"/>
            <end position="4450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="4455"/>
            <end position="4465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="4466"/>
            <end position="4475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="4492"/>
            <end position="4510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="4511"/>
            <end position="4524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="4811"/>
            <end position="4833"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR010340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4363471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="4916" mass="552591" checksum="73F93B78A3146C8C" modified="2023-11-08" version="1">[encoded data, 4916 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3G3</accession>
    <name>A0A9W8Y3G3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3G3"/>
    <dbReference type="OrthoDB" id="2130169at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051783">
        <property type="entry name" value="NAD(P)-dependent_oxidoreduct"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48079:SF7">
        <property type="entry name" value="NAD(P)-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48079">
        <property type="entry name" value="PROTEIN YEEZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="1">
        <location>
            <begin position="8"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="353" mass="38101" checksum="39576DAC6F7326C8" modified="2023-11-08" version="1">MPKLFFTGATGYIGGDALYVIANTYPDLEITALVRNSDKGAKVAAQYPKIRLVYGNLDSADLLTTEACNADIVVHTADCDHLGAAQALVAGLAKSQKKTFLIHTSGTGILSFEDFESGTYGIKREKVYNDWDGIGEVTSIPDVAVHRNVDKLILAASETSAGSIKSAIVCPPCIYGPGRGPDNQRSVQVYDMTKAALNRHKGFVVQEGANIWTEVHVQDLSEVFLALVTAALSPDGGKATWNDQGYYFAESGEFVWGDIGRNIAKIGHEKKLFNTADVDVVSKDEADKLRAAGSYLWGTNSRCKAIRANKLLGWTPKQKTLSQLLPEVVEDEAKKLGLIQGHAEVAAEGRILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3S3</accession>
    <name>A0A9W8Y3S3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GPI-anchored wall transfer protein</fullName>
            <ecNumber evidence="9">2.3.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">GWT1</name>
        <name evidence="10" type="ORF">N0V83_007751</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">A acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI-anchor.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Probable acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Endoplasmic reticulum membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the PIGW family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.-.-" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3S3"/>
    <dbReference type="OrthoDB" id="15270at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032216">
        <property type="term" value="F:glucosaminyl-phosphatidylinositol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009447">
        <property type="entry name" value="PIGW/GWT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06423">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017321">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 9" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="79"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="346"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU280819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4366116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="491" mass="54171" checksum="A408E50EB7AAD7FA" modified="2023-11-08" version="1">MAKTYKELKEAWVSGHTGSSVADVNSVSLAMPLSILLWSIIQNRMRLFTPYTPAAVLVDFLLNCGATLFATTIYSSSPWVLNLLLLLPAVILYVLEAPAPAKREAPRPPRDDDSKKDSKRDALPVKPFITNYRGAMMMITCIAILAVDFRVFPRRFAKVENWGTSLMDMGVGSFVFTAGVVSVRSALEEGSGRRPALSKRLLTSFRHSIPLLVLGTIRLISVKGLDYAEHVTEYGVHWNFFYTLGFLPPFAALFQTAFDLVPSYAGLSFALAALYEIALDWTSLGSYVLIAPRTNLFSKNREGVLSFLGYLAIFLAGQSLGTIALPRQQLLPKNASISTLLRQSTLGKLAGISVMWTALFYFSTGYYGLRLSVSRRLANLPYVLWVSSFNSYQIAICCAIETFLFPNLYKATTKDDEMQRSRDATSTVLHAFNRNGLAVFLLANLLTGLVNMTMPTLHMSVLQSMATLIAYTITLSAVAIGLDHCNLSIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y446</accession>
    <name>A0A9W8Y446_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SUN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_007685</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y446"/>
    <dbReference type="OrthoDB" id="266334at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000082">
        <property type="entry name" value="Sad1/UNC domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045120">
        <property type="entry name" value="Suco/Slp1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12953">
        <property type="entry name" value="MEMBRANE PROTEIN CH1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12953:SF0">
        <property type="entry name" value="SUN DOMAIN-CONTAINING OSSIFICATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51469">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040817807" description="SUN domain-containing protein" evidence="6">
        <location>
            <begin position="28"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="domain" description="SUN" evidence="7">
        <location>
            <begin position="268"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="37"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="114"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="497"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="850"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="965"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="37"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="529"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="552"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="573"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="591"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="644"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="976"/>
            <end position="985"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4367155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1011" mass="109749" checksum="888FBACAEE3B9654" modified="2023-11-08" version="1">MPTIGAPIRKWTLLLLLFCSIPTSAAAQAANETHTVESGATATASESTTAKHTPTTYLPSTSTAISPSPPPSRKYTDTETTCPFRTINYITHTLPQQCAKTSWSAPGEAAFANKTATEAEATPSPETSIPGDREEVEGKVEKEAWGSSSTAIPRAEEAGHGESATASGTSNIEAEAELETDSPFDNANFLSFEEWKKKNLAQIGQSPENVGQGRAAASNQPARRRPVNVNALDSLGDEAEIEIDFSGFGTPEGENVAKNIQSETREPGATKASDGAGKVGPSSWALSKDAGKTCKERFNYASFDCAATVVKTNKQAKSSSSILVENKDSYMLNECSADNKFLIIELCDDILVDTVVLANYEFFSSMFRHFRVSVSDRYPVKMEKWRTLGTFEARNARDIQPFLITEPQIWARYMRIEFLTQFGNEFYCPISLVRVHGTTMMEQFRREEEEARGVDDDEELDETTQIDLVKPAADSGPLPPDQIPIQPVKEYNSISDTVPVENASPPVRFSDASTEYPQAEETQRASELPTAQTGSVRSSQATDAQRHDEPVATTSMHSSPATTSNAVDDKKNNTSANSTQRSETPSSLNRTEASPLTTSSASSSPIATPSPSASGNDNAVSSVANATASAPSSNSSIKGSSNSTVANAQVQPQSRGSPTQPNPAAPSTQESFFKSIHKRLQYLEANSTLSLQYIEEQSRILRDAFVKVEKRQLAKTEKFLDHLNSTVMQELKSYRTMYEQLWQSTIIELEGMKERQKVEVGEIGTRLSLMADELVWQKRMAVVQSTLLLLCLGLVLFVRSGTIGSTSDVPIVQQLGSKYTSFFESSPPRSPAVSGMARRRRTFRNMWRSDTSAGLSDHQSDGQHVLSDADTDGARSPDVQIDYSPPTPDTPGALGAQDALLDAQVNGIGTSPAQESDAEFPIASPDDQANRVQVLETQSGPATPNGTRDSKPSWEEVDRAMDQLKAEQQGRSTLLSKKKKKRSPLRRAQSSYDCTVDDSAADNSGDGLFVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y450</accession>
    <name>A0A9W8Y450_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_007300</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glycosyl hydrolase 43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y450"/>
    <dbReference type="OrthoDB" id="272289at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18820">
        <property type="entry name" value="GH43_LbAraf43-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006710">
        <property type="entry name" value="Glyco_hydro_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43817">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43817:SF1">
        <property type="entry name" value="HYDROLASE, FAMILY 43, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01660)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04616">
        <property type="entry name" value="Glyco_hydro_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity, responsible for pKa modulation of the active site Glu and correct orientation of both the proton donor and substrate" evidence="5">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606710-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4366775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="276" mass="29939" checksum="DA4DAAFA278F37E5" modified="2023-11-08" version="1">MVGLEDYQGEDAWRGLKTGETKTVWTDTNANRCCNMWAPELHYFDGTWYIYYTAGRSGSDNLDLQRPYVLKGGADPFGTYTYHAQLSTTWGIDGSIVRFSTWGNYFVWSCMSNSLQSLCIAPLTAPGKIGATKVLSQPTQSWEKNGSPVMEGPAAMYHGGKTYLTYSASFCWTPNYSLGLLTWNGSGDPALGSSWAKSGPYLTSANGNYGPGHNGFFNSPDGTEIWNVYHATSISTGSCDGNRYTMAQKVNWNSDGTPNFGSPAKLGTTIAGPSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4A3</accession>
    <name>A0A9W8Y4A3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_006864</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat WDR48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4A3"/>
    <dbReference type="OrthoDB" id="2421129at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051246">
        <property type="entry name" value="WDR48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021772">
        <property type="entry name" value="WDR48/Bun107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19862">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19862:SF14">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11816">
        <property type="entry name" value="DUF3337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="100"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="193"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="233"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="592"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="651"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1024"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="620"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="656"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="695"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="709"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1029"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1049"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1062"/>
            <end position="1077"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4367283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1089" mass="117507" checksum="E9FAE04A566998E9" modified="2023-11-08" version="1">MGNWTDKAPYSAATCKVGGRTQTRSQWLGCRPPELHPVRNPSYIAAIHANVPSYSGGRDGVICAWDLHLDLSKSADERDPFADSNASNYKPPPTQFRQQVQAHTHWINDIVLAQNSNALVSASSDITVKVWRPAAADPLPPQTIGLHTDYVKRVASPGGNENWVASGGLDRKISLWDLNGAGKKLEIAVGEDENTAKGSVYALAVTPNIIASGGPESIVRVWDSRSGKRITKFVGHTDNVRDILVAEDGDTILTGSSDQTVKVWSMTAGRCMYTLTMHNDSVWSLFSDCPQLSVFYSSDRSGLVAKSDVRNCEEMDEGLSVAVCQEHEGVNKIVAAGEYVWTATSSSSINRWNDVDTTADVELPESYKWHRSSISTMRSRLPSPPSNSPPTNGTTAKIPLKALLRMSNTAPFPQAFAIKDSDSTTLSSTRKTPEALVASDLGVGVPCRNLPDFSIEGQNGLIKHHLLNDRRRVLTLDTAGEVMLWDLLQCAPIKSFGKKHLEDVVPEVQTRESVAHWCGVDTRIGTLTCVLEENYCFDAEMYADELQLDEQIDFREDHRINLGKWILRYLFSNLIDEEIKRDEAFRADLLKQKEEQPKQSRPTNIQLPDVNINGWKDASSGPTSGSTIKANNGFHLPHTPGLAIGLATPMAAPQSAGRNSQHNPYLTPTTEEGSQLELTQTRRSTTQEGGDYFGQSASNNNTNGNGKSADVKEDSTDSKEEVVSSPTEPDTPAKKGKGMFGKKFNMSFNMKKFSTGTAIAEPVKPTTVDEKAEDSDSHSTKTDEKVFEDNFLGTLQRIRQGYEEQLGAGAAKLESQIAPSLPSDTPVLKPPASTTILIQEDRPEAGGVADLFEGKVGSLGLQADLIEKAAPMWLAEVLLRNQLPPKDIVKVSFILEPHQNALPSISADGYIPFSLPFETASQANATLSNNRLNANRMLRARKILGYVAERIEPAPSKEEIAARAAAEAGGAAGGGVLRPEEYLELYCNGQLISPTMTLASIRAHVWRGGGDVLLHYKANGRKEIKHAPYPGPQSGSPPGPIHGGFAAAIGGGGAGGLGSGPGSSSASEAGRSSTQSSRGVVEGKSVFGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4D8</accession>
    <name>A0A9W8Y4D8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4D8"/>
    <dbReference type="OrthoDB" id="3788615at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="318" mass="35072" checksum="7902B25EFAE900B3" modified="2023-11-08" version="1">MEAQEQGKKGPFGKKELKLLYATYSEADLYNSGTFKPLTRNLTTGAKLGLGHHCQQCNGKMTQSCYENLHYAYCATWVNRMLDGKPVRRRCGERFALRSDGCGRHPRVQGYNEPLYRAAEGYDVALSEFDDPDPDNSKGEPEDDEEDWEAEHIELARLTEEEIAKNGFLPADFHNSYWQALRQEQAFAKSKKRRAVETGSEDVGENPTTNGRGQANVVANGKAGAGKKTQNRAITFADQPHEKHGRGASTYAGTVTADKPKRSYAPKGSKPKAPMEVPRRPAAGSNDENANNEGKGKGKTRVGTWWSKKKNGNGGASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4H9</accession>
    <name>A0A9W8Y4H9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MFS general substrate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_007500</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4H9"/>
    <dbReference type="OrthoDB" id="5376138at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000088">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF3">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="326"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="373"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="401"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="506"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="509"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="526"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4366970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="546" mass="60816" checksum="E8C485976CC33BCC" modified="2023-11-08" version="1">MATVEKEQPTAVPETERDPDHGQGEDLEQEKTDRIVKGDKVELQDSDAWDKLGYSFPTWRKWQILIVVFFIQISINTNASMYSHAVTGVSEKYGVSEQVARIPQMAFLIAYAFGCELWAPWSEEFGRWPIQQLSLFLVNIWQIPCGLANNYATYVVCRTLGGLSTAGGSVTLGVLADMWEPDEQEFAVAFLVLSSVGGSVVGAIIGAFVEAYLSLPWIFWVQLIIGGFVQAVHFLCNPETRSTILLDREAKRRRKSGEDPNVYGPNEIRGGHKITFKEVMTIFWRPFYMFFTEPIVLWLSLLSGFSDALIFTFLESFKPVYSQWGFGTVGIGLAFVPILIGYFLAYFSYLPSIINFRRKRRQNPGSVTPEARLWWLLFLAPLETIGLFGFAWTSLGPSHGIPWIAPMIFSTLIAMANYAIYQSSIDYQTAAYGPYAASATGGNDLARDFLAGVAAMYSTPLYTNIPGRPVQYASTVLACLAFVVTIPIYIVYWKGPEIRENSKFAQSLDKSRAEREEKRGQRKGSLVGGGGGEDGGRIIGRGCEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4P3</accession>
    <name>A0A9W8Y4P3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Something about silencing protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SAS10</name>
        <name evidence="7" type="ORF">N0V83_007061</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAS10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4P3"/>
    <dbReference type="OrthoDB" id="1924577at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007146">
        <property type="entry name" value="Sas10/Utp3/C1D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018972">
        <property type="entry name" value="Sas10_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237:SF8">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09368">
        <property type="entry name" value="Sas10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04000">
        <property type="entry name" value="Sas10_Utp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sas10 C-terminal" evidence="6">
        <location>
            <begin position="578"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="462"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="591"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="60"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="465"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="594"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4367478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="655" mass="72474" checksum="E8E54FFE1497D6B1" modified="2023-11-08" version="1">MAKKRKAGGRAYGEPSKQKDSDSKLAISTWEDVADSEDEFHINRDKVLLDEGPTAKRLRKWQEEDKFLQHSDEEVLDAGIDDDNDDESDLDDAEEVAESDDGSAAERGPNAEEEDEDLGGWGASRKDYYNADAIETEQDALDEEAEARRIQKKQLQAMTEADFGFDEDEWLAQDGEKDAEGGAVVTEVLPQLQITESMSEEERLKIMRTRYPEFEHIAREYTRLQPLHDELAAAANAAERLLKAQAAKSAKANRKITSTPMAVTKYRACAAYLAAMAMYFAVLGSTAKEDGTPVIALEPAELHEHPVMESLLKCQQMWTRIETLPVGDPMVETDIRDDLSVIEEDSPVTNIELDAATTRTKMTKQKKSRAQRAAEIAQAEAAARRAAKLAKADQDLASLDSLTDKAALKKAARKKKTAEPKLNLLNAEDSDFGEETELTAHELAEKTKKKKSLRFYTSQIAQKANKRDAAGKDAGGDADVPHRERLKDRQARLQAEAEKRGQNKAGPGVTLGEDDGASSADEAPAARKDDFEDEEGYYDMVAARSNAKKQTKLDRAAAYALAAQQGAQVIEEEVVGDDGRRMISYQIAKNKGLTPHRKKSVRNPRVKKKEKYKEKQKKLKSTKPVFDRAAAAKGSANYGGELTGIKKGLVRSRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5S6</accession>
    <name>A0A9W8Y5S6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Synaptobrevin homolog YKT6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_006691</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5S6"/>
    <dbReference type="OrthoDB" id="190375at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.50:FF:000017">
        <property type="entry name" value="Synaptobrevin-like protein Sybl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000004">
        <property type="entry name" value="Vesicle-associated membrane protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051097">
        <property type="entry name" value="Synaptobrevin-like_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136">
        <property type="entry name" value="SNARE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136:SF168">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="214"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="11">
        <location>
            <begin position="10"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="150"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4368335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="245" mass="27335" checksum="4831FC1B1B5820C0" modified="2023-11-08" version="1">MASSSSSTPLLYACIAHNTTVLTEHTASASSNASSLVSLVLPRLTHTTSSHKTIDQSKYFIHCLVKSPSDFPATQASAGGLTFLVIAERDLGQRIPFGFLTNLEKKFFAEFETSETDFADLPPYGCASFNGSLKKLMLEQGSTQAGQQDALRTAQREIEGVREIMTENIERVLERGEHMSVLVDKTGRLGENARDFRVRSRNLKRRMWWKNIKLMVLLCLVVIFLIYLFVGFGCGLPAWGRCLRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y621</accession>
    <name>A0A9W8Y621_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LYC1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y621"/>
    <dbReference type="OrthoDB" id="2020070at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055100">
        <property type="entry name" value="GNAT_LYC1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053013">
        <property type="entry name" value="LAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34815">
        <property type="entry name" value="LYSINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34815:SF2">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22998">
        <property type="entry name" value="GNAT_LYC1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LYC1 C-terminal" evidence="1">
        <location>
            <begin position="190"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="430" mass="47699" checksum="8348B19C97BFB40E" modified="2023-11-08" version="1">MAAHKYNKTLPDPHSPDLILSHPTSNEHITIWTSTSALWADSLTTLPLYIKESQRLATAPLARDGGLTTWILVDGNRPADQREILSSCETFRKRALTSDVEGNVVENIVHGVASVFCPEEYRGHGYGSRMMRELAKVLSSWQTGGDGGGKRCIGSILYSDIGKSYYAKLGWHADKSNTHVEFSPCAHPRSALMIKDVRVTDLEALCKRDETLLRKLMARPASTTCTDARLRMTIIPDVEHMGWHLAKENFACEHLFSGVVPQAKGAIAGDVVGSQVWAIWTHRYYSRPPHHGGGDCVDSSSQQQTNNVLYILRLVMEADETASRLPSDATKTFLEPAGNGEKYAQQMGYLKAVLQAAAAEAREWKLDVVKLWDPTPLVRGMLMKMGDLGCVEVEREDEGIACGLWYDEQGEVLGGEEGMVWVNNEHYAWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y664</accession>
    <name>A0A9W8Y664_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005839</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y664"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11069">
        <property type="entry name" value="CYP_FUM15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF166">
        <property type="entry name" value="CYTOCHROME P450 12A4, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="2">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="40824" checksum="05130DE113B66239" modified="2023-11-08" version="1">MPAFHFRHIKELYPVFWSKSIELCNAVKATLDHNTTQVLEIGHYSTQVTLDIIGLAGLGRDICSLRNSDDELIKNYEEILEPTTEKGVFFILHLIFPPWLIAILPWKLNERVRITTGNLKRICTEFVAERKSKMRYEDQSSSESRDILSIMIRSNNFSDVNLVDQLLTFLAAGHETTSSALTWASYLLSQHPEVQTRLRSEVHTSISDAKTLSDPNFDIADLLESMPYLNGVCNEVLRLYPTIPVTARVAIRDTTVAGHFIPNGTMLFVVPWAINRNPALWGPDAGDFVPERWIDKETGRATMNGGSDSNYAFLTFLHGPRSCIGERFARAELRALLAAFVGSFEMELANPHEKVVVGGTVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y673</accession>
    <name>A0A9W8Y673_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">S-glutathionyl-(Chloro)hydroquinone reductase</fullName>
            <ecNumber evidence="5">1.8.5.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ECM4</name>
        <name evidence="5" type="ORF">N0V83_006536</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.5.7" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y673"/>
    <dbReference type="OrthoDB" id="2309723at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03190">
        <property type="entry name" value="GST_C_Omega_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000162">
        <property type="entry name" value="Glutathione S-transferase Gst3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047047">
        <property type="entry name" value="GST_Omega-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016639">
        <property type="entry name" value="GST_Omega/GSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419:SF6">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE OMEGA-LIKE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419">
        <property type="entry name" value="GLUTATHIONYL-HYDROQUINONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015753">
        <property type="entry name" value="GST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01206">
        <property type="entry name" value="Xi.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01148">
        <property type="entry name" value="Xi_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="4">
        <location>
            <begin position="49"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="122"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="140"/>
            <end position="141"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="3">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="3">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4368180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="334" mass="38983" checksum="C296D62256960592" modified="2023-11-08" version="1">MAITDWVKPGDSSGEFKRQQSVFRSWIENKPDAEFPPEKGRYHLYVSYACPWAHRTLIVRQLKGLQDVISYNSVHWHMGEKGWRFATPDEKLPGETTPDPLHEGFTHLRDVYFEQNPGYEGRFTVPTLYDKKTNRIVSNESSEIIRMMYREFDGLVEEKYRGVDLFPEKLRKDIEEMNDWVYNDVNNGVYKSGFATTQEAYESAVTTLFTSLTRLESHLSTSSTPYLLSAPAPTEADIRLYTTIIRFDPVYVQHFKCNIRSIRSGYPHLHKWLRHLYWDYPAFKDTTEFEHIKKHYTRSHSQINPHAITPVGPLPDILGKDDEVEAVKAVLEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6J4</accession>
    <name>A0A9W8Y6J4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Lipid droplet-associated hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_007521</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. LDAH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6J4"/>
    <dbReference type="OrthoDB" id="448051at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019363">
        <property type="entry name" value="LDAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13390">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13390:SF0">
        <property type="entry name" value="LIPID DROPLET-ASSOCIATED HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10230">
        <property type="entry name" value="LIDHydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4366991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="39913" checksum="8F74A10689A2E43C" modified="2023-11-08" version="1">MTTPPSSKIYLRTPPEAGKKSAARTYIIYFLTGNPGLVEYYRTFLTHLYGLLSRDTASNRDVEFQVYGRSLSGFEMSTSEIKTMKWRKQPPYGLQDQIRHAEEDVVDLVEEAKNQGGRDVRVVLVGHSVGSYISLEIIRRLRAHGMAGEDFDVRIVGAVGLFPTVVDIARSESGIKASPFLKNSNFATIASIIVSFLTFLLPVSLIASLVARFAGFPGDAAHTTASFIKSPFGIQQALHMARDEMFQIDTDIWDEDIWGATEPSKHPHPRPTLRFLFARKDHWVADATRDALISSRGRFSYGGVEETDGMGENWKPIMEIDEREGWPHGFCISHSVPVAEKVAGYVRDIVGKDMQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6V9</accession>
    <name>A0A9W8Y6V9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6V9"/>
    <dbReference type="OrthoDB" id="506431at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052061">
        <property type="entry name" value="PTE-AB_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260:SF7">
        <property type="entry name" value="THIOESTERASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_1G10800)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260">
        <property type="entry name" value="UPF0644 PROTEIN PB2B4.06"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="1">
        <location>
            <begin position="114"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="221" mass="23523" checksum="BF39D5B958CC29FA" modified="2023-11-08" version="1">MAGAPALESLRDIANPPTDEETLTLFQPSSAEIAAIEDSILTHPLTQSLLHDPKYIPSRPHLKIPESLRAQNLTGGTLLGADKIAVPPLQFSTADGTSFVSLQYLGSALCGHPGIVHGGLLATLLDEGLARCCFPALPNKVAVTASLKIEYKRPVMAGQVVVLRAETVKVEGRKAWVKGWLETLPQGVEGMGREREGGCADGGEALFIEPRQAASMRRVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y732</accession>
    <name>A0A9W8Y732_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_006322</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y732"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF2">
        <property type="entry name" value="MANNOSYLTRANSFERASE KTR5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4369237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="397" mass="46444" checksum="430D20426915090F" modified="2023-11-08" version="1">MSLTKLNRNHLRRVLLFLAQHWKASLGIILLMTIEVIFHIRSFTIPRPSTNLDPPFYTTCHDPSTMNSTARANATFMMLARNSDVSGAVQSVKSVQAQFNQHFGYPWVFLNDEEWSEEFKMEVGKAVGEGVSVTFDTIPWEVWGFPMWIDREKARRKMKKMEREGVMYAGEESYHHMCRFQSGFFYDHPALLPYKFYWRVEPDIRFTCAIPYDPFLSMSQHDKKYGYAIALWERGNTVPSLFRKISDYKARRHVRTTPLWTALMAPSYAPWLIRRFVLSLLRNRDGKGDLWNMCHLWSNFEIADMEFFRSSAYRELFRYLDEDGGFYYERWGDAAVHSLGVAMLLRPDEVHWFSDWGYVHGGLQVCPKEGLGCGCECEEGGREVETVCLDSIRRTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7C2</accession>
    <name>A0A9W8Y7C2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Actin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ACT1</name>
        <name evidence="10" type="ORF">N0V83_005472</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7C2"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000050">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000131">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000001">
        <property type="entry name" value="Actin, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00406">
        <property type="entry name" value="ACTINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4369709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="337" mass="37692" checksum="7E212CE905AC32A1" modified="2023-11-08" version="1">MAPTIMIGMGQKDSYVGDEAQSKRGILTLRYPIEHGVVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPINPKSNREKMTQIVFETFNAPAFYVSIQAVLSLYASGRTTGIVLDSGDGVTHVVPIYEGFALPHAISRVDMAGRDLTDYLMKILAERGYTFSTTAEREIVRDIKEKLCYVALDFEQEIQTASQSSSLEKSYELPDGQVITIGNERFRAPEALFQPSVLGLESGGIHVTTFNSIMKCDVDVRKDLYGNIVMSGGTTMYPGISDRMQKEITALAPSSMKVKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7L7</accession>
    <name>A0A9W8Y7L7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWIRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005608</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7L7"/>
    <dbReference type="OrthoDB" id="5598695at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000087">
        <property type="entry name" value="Transcriptional adapter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374:SF21">
        <property type="entry name" value="SWIRM DOMAIN-CONTAINING PROTEIN FUN19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTOR 2 ADA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SWIRM" evidence="2">
        <location>
            <begin position="283"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="242"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4369844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="380" mass="42737" checksum="1FCE903D47716D93" modified="2023-11-08" version="1">MANSPYKTPPSHRLTVSSLLSPPEMKRSESFGSSTAPAFAQSPFSSFNSVSSKPEADIMAYQAAVHATREAYASPPISPFDTQSQKENMDIAEDQGPRDPQLFVSNDTAIPVANVPLFPQEAAEPASQDLITEHMKSSDYAQLESKPSREDYELAISFRSTVFDQVVKNPRHWWEQERRFDQYYGRPSGVQKRPALKKLAPAPSSRPRQPKVALPRLPPRTPRAPKAKRTPLAQVYESFEYAPASASPKPARPATNRDDTDYNALPDYSPPLATLPKGNNKSLKAEWKGTVLDLSNDPDRHLLHEAELHLAATLRLNCATYLCSKRRVFQARIEALRIGKEFRKTDAQQACKIDVNKASKLWQSYDKVGWFNPDYFRQHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y865</accession>
    <name>A0A9W8Y865_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_006751</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y865"/>
    <dbReference type="OrthoDB" id="269227at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF134">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE OXIDOREDUCTASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00623">
        <property type="entry name" value="GMC_OXRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="5">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="524"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="568"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="94"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="523"/>
            <end position="524"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4368394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="592" mass="64687" checksum="FE2EBE3D18831933" modified="2023-11-08" version="1">MAADYDFIIVGGGTAGCLLAHRLSHSAAKPSILLLEAGTNPSGDYLRAPFHRYTPAMLRPDLDQGYTSEPEPGLDGRQIAYTRGKGLGGSSILNFGVYLYGSGEDYNRWSELVGDESWKWESVKDSFHEIEDYQFNTAFAHKHLADPSGNQHGRSGALKVGVPPLLERGVEEQMEAVLREGEKLNLDPNDGDPVGVSIFPSSYGKEGRTTSAIAHLVDSPANLEVWTDAKVHQFVWEGMRVVGVVTGDGRKGEFPCRVPPVSYDHFNVRVGAYISTASATKEVILCGGTMDTPKLLLLNGIGPRAELEALGVQVKQDLPGVGKNLQDHVMTFMSVEVDGKSNDRYSFESNAALVAEAEKAWAQDQSGAFALHQSAVWGGFLKLPALDTYPEYRALPREHQAFLSKAAVPAYEFCSNALLWPPGAQLEPGNTYVTFLAFLMNPQSEGSVTLKSSDPEDKPVIKLNYLTHPYDARVMREAIRETWTLIAENPSIKPTIRKTLCGPASLLDEDIDAFIRATAGTVWHANGTVKMGREGDEMACVDWRFRVRGVKGLRVADLSVCPLTTNNHTQATAYLVGKKAADRLIEEYGLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8E1</accession>
    <name>A0A9W8Y8E1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8E1"/>
    <dbReference type="OrthoDB" id="5431222at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="443"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="544"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="603"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="632"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="664" mass="72969" checksum="90415D44414386CD" modified="2023-11-08" version="1">MVMLNPRMANSNPINMDSSQRMDNIRQGRLIPKVAYPKVAHPNGHQIIGKQSINLPLHALRRTLTIVSPPKKKGNPIITRYPPPPGYRGPAQPQGPFGANQYANQYQPPQSGYPQAPPAPPTCPQQGYTVPPTRQGYSPQGYGQPQAPNYPPPSYPQPQNYQWPQQGYPPNQGYSQHQMHSHVQGYPPPQSQNNYPGYAPQSANVDPNQQHYTQPQGWSSQAEPSQYPPNAQYNSYNGPTGNPPPLSDLNAARTTATAPLAMSQPPQPVNQPTSAVSENGSSGKPHLDLGCDDWDFEFDGAIWPKTNELVDPNLSLGVIIWHPAKQMTRALPSTFAEAEEQTLKPTPEKLNNGESVSMYFMAENSHEAFLDVRQTDDWEQIRDDPVFVVFTDEEMQHNLVSLEDCIAQGDRDDEIPEGARLDEDQEMHDATGDIMDNLEQALSWSNGSSKPAGPKREAISSPKQSQEDILAMLGVTGAPKTPSDEPISLSFSSSQDVKPPISLPQKPPLVSPSLTRPSSPPPPPRAQSFGGPRNPGYSVPPRPFGSMSSSSNSRPPPPPPPPEEQRYDPWNAPQRNGPGLDGSRGSPALSEGSNRTMAGSDFESEKPSHGTDQEPTAIPPLQRSDSSFARKRSYEDTDHDSEMMQQQDDNSKRKRRAQVESAYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8T0</accession>
    <name>A0A9W8Y8T0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_005226</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8T0"/>
    <dbReference type="OrthoDB" id="16820at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051104">
        <property type="entry name" value="FAD_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46720">
        <property type="entry name" value="HYDROXYLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01460)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46720:SF1">
        <property type="entry name" value="HYDROXYLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G06050)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="4">
        <location>
            <begin position="9"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4370705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="421" mass="47307" checksum="9BCBF386F1FB330C" modified="2023-11-08" version="1">MTSSTTPKSVSIIGASVGGLTLGLALKKYGIQPRFFELRNPDQDLGGAMSLTPNSLRVLESIGAYSRLKPQGFDCTAFTFMTEPEHEITGELYFGQKDVYGYDCLRVTRKVIIRELTEMVKEAGLDIHYGKKFTKIVHEDANGVEFEFADGTREKDEMLVGADGIHSKVRSSVFPDIHPHYSGVLGLTYCFPRANWQHLEETFPLPCSLRGKQGSWIITPQTEGGQEIFVGRQLKYEQEDRSGWDALLKNKQAMIDMIQRDPDDWSPLMQAAQAQVSTPDAHFLNVWPFYTLKMERWHSSTGRVVVIGDAAHAIPPSAGQGANQAFESSYSLAMLLANLNGKVNLPDALMAWEAYRMERLDDVIRLTNRVMTLRMTEEERASMPEEMRWELDGTDAGKSQLGWLFLADIDRDVRNLVKTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y979</accession>
    <name>A0A9W8Y979_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Acetyl-coenzyme A synthetase</fullName>
            <ecNumber evidence="3 8">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ACS2</name>
        <name evidence="12" type="ORF">N0V83_006017</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23176"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="3 8"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y979"/>
    <dbReference type="OrthoDB" id="1706066at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05966">
        <property type="entry name" value="ACS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000004">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000001">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF14">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="11">
        <location>
            <begin position="46"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="9">
        <location>
            <begin position="104"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="10">
        <location>
            <begin position="551"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4368935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="671" mass="74714" checksum="CB90909E75AFA63D" modified="2023-11-08" version="1">MSEGDTKPPSAPVVAEAHEVDTFHVPKAFFDKHPHSKPHLANLEEYQTLYKESITEPKKFWGNLARELLTWERDFQTVHAGSFEHGDNAWFLEGRLNASFNCVDRHAFKNPDKPAIIYEADDTADGRIISYGELLRQVSKLAYTLKEMGVRKGDTVALYLPMIPEAVISFLACSRIGAVHSVVFAGFSSDSLRDRIIDAECKVVITTDEGKRGGKTISTKKIVDEALKQCPGVSHCLVYKRTGTEIPWTKGRDWWWHEEVEKYPNYIAPEPMNSEDPLFLLYTSGSTGKPKGVMHTTGGYLIGAAATGKYVFDIHDNDVFFCGGDVGWITGHTYVVYAPLLLGVATVVFEGTPAYPNFSRYWDIVDKYNVSQFYVAPTALRLLKRAGNEHVKHQMKNLRILGSVGEPIAAEVWKWYFEIVGKEEAHVVDTYWQTETGSHVITPLGGVTPTKPGSASLPFFGIEPAIIDPVSGEEIHGNDVEGVLAFKQPWPSMARTVWGAHKRYMDTYLNVYKGYYFTGDGAGRDHEGYYWIRGRVDDVVNVSGHRLSTAEIEAALIEHHQVAEAAVVGINDELTGQAVNAFVALKEGNESSDQVKKDLILQVRKSIGPFAAPKAIFVVPDLPKTRSGKIMRRIMRKILAGEEDQLGDITTLSDPSVVEKIIDIVHQSKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9B8</accession>
    <name>A0A9W8Y9B8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004338</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9B8"/>
    <dbReference type="OrthoDB" id="5411773at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="516"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="575"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="594" mass="65539" checksum="8704818B631A181C" modified="2023-11-08" version="1">MSAFATPESSRKISQRSLNCNSSNTPSAALEAVGSRPRRAQRRPTKQASYELANHAKAYLEGGQYASGYDFLSSLLAASTSISTPAQPYVGYLAPPAYIAFASSLVVYPHITTQAQSKDAIKGSDAALRYLQCVYSTIDGPAHPTICKAFTFPEERNRRRVPGYRSAAASLSPEPSGDIEQIAGDDAHAKSLWYRAEDFWHIVGWAFNCSVAHKKRWDRWKLWLDTSLDFLEMDWDTCIKQSSGNGGGRASILQESLLWHYIVGDAESTNRGMRRRIVKAILAMASPESQKEYPEVWEKETAEPKRKKDHNQPLGKVDFETGQVGDYDNDEEMQDASEQASDEPTSSPTVVSDGGIQSLDDAVEQLGGQDAIALRQRLIALLAQVALSLPAQFTTLSDYFDNILEDFIHLPTIIFKILLSTLKMPERLQIAFNINLLLPLVNRKVPEYFHHEPTQEHFEATLLPLKGTTQSYAANAKISLILEQMFMYMMTCNALTPTDALRQAMETGIAARHSVYGTGRGKRGHTEEEQQSQGLMEASSERLLGLLEVLEISAGKAPQPLKNKGKAGNIPVMLSFGSGSSLSPVPDSDIELDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9U4</accession>
    <name>A0A9W8Y9U4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004654</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9U4"/>
    <dbReference type="OrthoDB" id="73788at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="498"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="599"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="707"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="810"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="440"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="559"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="666"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="714"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="820"/>
            <end position="829"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="829" mass="91677" checksum="42B2FAA030021E52" modified="2023-11-08" version="1">MTPRTRKSNTDIDYKVYYSKKVPQQVHFPHKRKTVRRRSTPVPDGADKRQMVSVPEKMKAKNTKIIKDSDTESASEDEDLEEDGGVLLDVEILQEAEQSTTGVAKGKGRKRKSDTMNEYSEENEELVLPTPKRRRKVAAKKPQSRPRPIEADSEDEQDAKSLKVKSADNKSRRLRRQSTLTQLVDGRQASPHSEEPEFKPVKRSGRSTKKGKDKQQRTLTQMIPGRRSFEVVSDEDMTEALSDLETEEQGSADYGDVIAQRLAQQGLHHAEGDVPTFKREDGEEVHASAQSLLALGVQTVEPDLVKNEEESYQPTQYIDAPVTRTGPTRSRKSKQINKEKTVDTKLPVSRKSANSRFGLLSTPEKRRICEIPSSQSPADSPLSVQMSTHKTCRSPLKERSGNAISAPETPSKRKQVAFAEPSKEHHSPPTLRRFQSTVQDSEEEDEDIIEDSSSCAQWITASTKPGTGGIFKLGEAVGVNTQAILERIDQACARVDEDAACQDSDSSEELGVQATTRRDHEASPELGEQQPSLNRTRCRTASVEIKHELLDDSEVLPGNTVNSFSSPGEQAVASREPPNEHVELPTSVVEVHSTPPFSDAYLQDTFPSTPMVIQGESSDEDDAADSMPPSRAKQEPRRPPSSSRRQPSVELHDESVRVPLSPVAQRETQQSHSSKAEQQLQEEWLSYSQYLGTRPLESSSMHVAADAFSYNATPKPPRPAAAPPPPQHSGHGLSQATTVDEVTPKKNRRQRTISANVTPHKIASSQPFISPSKPPPLFIPSSFPSPAKAVMEGWSSPVYGRTQDFRSSQYGASLEDFSIPAPPPPLEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA19</accession>
    <name>A0A9W8YA19_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA19"/>
    <dbReference type="OrthoDB" id="3767399at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="432"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="186"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="509"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="526"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="550" mass="61948" checksum="9A4D3D2257658C25" modified="2023-11-08" version="1">MAAVAQSRIQDDVHPLLRHPKPIKRDSAKAQRMLGLIADDEEKLKALQREKSVTTRWLERPMYEYLEVSDVESEREETGHEQKVDSVHEERIGEDDPELIEQWTNPTRPTSYSSEASIRIEAKPELDTSFVKRRPDPINCPRPISYNAQHLLSPEWTVSPVAMSPNTQRPRPIPIQYNNSRTGRGSFSSNGSLESSPRIVHPQSWGAPSTQYKTNVRGERPVSYQPQSFASSPMATEPRPRPTSFATYPHQRNRSHTKIASSRGLRNNSYPNFSRPISEIAPKAVPGEQMENDSLYQRFGDDEVGPPTPHTPDRAPLSVFEPYEDKNTTEKKPKKRWSAIPQTFMKLTGRRASAAVQEPPKFEVKIDDLPQTNLTEQNLHSYEHEHEHEHKHEVNQAPPTPKTRLSGIDLIPTPTYSPFDVEHPLFEPELPLPFAPWADGPPSPASSSDKRRGSGTSSLSPTRKAAPSSRMSVDHAASGRPASLHSRRSSVVALSPTSTAPPQRPPMDVPVSPRTTPSRRNTPTLEQRDVEKWTQLEFKDIREGQKSNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA34</accession>
    <name>A0A9W8YA34_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zinc/iron permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_005622</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA34"/>
    <dbReference type="OrthoDB" id="448280at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF24">
        <property type="entry name" value="FE(2+) TRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="360"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="404"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4369858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="422" mass="46001" checksum="983CBDDB639A6A82" modified="2023-11-08" version="1">MSEMEKPVCGSEADGAAYDFPLHVAAVFIVFFASIGGCGFPVVAKKVKWMKIPPKIFFFCKHFGTGVLIATAFVHLLPTAFASLNDPCLPDLFTDKYPAMPGVIMMGSLFILFVIEMWLNAKTGGHSHGSATGQEFAGGQAPPGIQAAFNNPIRRTNSYDSQKTMLAMRDEKRGWVEESYPVDNFPFPKNGSDELEAKSEMPPWFIVFYEQYVRQRDEMLGMLNRAMPALPSYPQQTVQETTPNSYFEDDVEQQAVDPMALKKQSMQITLIEGGILFHSVFVGMTVSITAEGFIVLLIAIVFHQMFEGLGLGSRIAAVPYPSKSWKPWALVVAFGTTAPIGQAIGLLTRGSYDPNSAFGLIIVGVFNAISSGLLIYAALVDLLAEDFLSEEASHTLTGRDKTMAFVYVLMGAAGMSIVGAFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA61</accession>
    <name>A0A9W8YA61_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein atg6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">atg6</name>
        <name evidence="6" type="ORF">N0V83_004115</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA61"/>
    <dbReference type="OrthoDB" id="20368at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043548">
        <property type="term" value="F:phosphatidylinositol 3-kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.40:FF:000005">
        <property type="entry name" value="Autophagy protein Apg6, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.40">
        <property type="entry name" value="Autophagy protein 6/Beclin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007243">
        <property type="entry name" value="Atg6/Beclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038274">
        <property type="entry name" value="Atg6/Beclin_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041691">
        <property type="entry name" value="Atg6/beclin_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040455">
        <property type="entry name" value="Atg6_BARA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12768">
        <property type="entry name" value="BECLIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12768:SF4">
        <property type="entry name" value="BECLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04111">
        <property type="entry name" value="APG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17675">
        <property type="entry name" value="APG6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Atg6/beclin coiled-coil" evidence="5">
        <location>
            <begin position="180"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Atg6 BARA" evidence="4">
        <location>
            <begin position="311"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="37"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="107"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="231"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="42"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4372341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="531" mass="58595" checksum="A92F229D1BCE8AE8" modified="2023-11-08" version="1">MANDRKGEGLSCQKCRAPLDIDASIDQLNPAAFKLLTDSAVPGQQQASKSSTNRQSRPTYPSSRQETYETALQHARNPTFKRNVPSSRFQHGSQNPAMSFVNVHMSESMLDPPTPSPAPVRSPDRQAPAEDTQPSANEPTRRASVTGSTGAVGGASMADSLETTNRMFEILSARSDIDHPICAECTELLVDALQKRLGVATRERDAYVDYLRRANTDVPSTEEVKAAEAALKAAKKAEATAISNLEKLEAEKAELDAQLTALEVESRQLDQQENDFWKSRNDFNATLIEFQNERDALTTRHAHDAQILNQLQRRSVYNDTFNITHDNHFATINGLRLGRLPSPYVDWPEINAAWGQTCLLLATLAERLGYKFQGYELCPMGSTSTITRLEVKGGSSVAESRQSSATTPSVSKQRLELYSSGDFPINFGFLHRKFDTAMVAFLECLRQLGEFVENQGIQGVHGIGAGLGGPGGPGVKMPYEIRKDRIHDQSIKLALNKEESWTKACKYTLTCCKYLLAHANNVESMNARRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAJ4</accession>
    <name>A0A9W8YAJ4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Histone H2A.Z-specific chaperone CHZ1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">chz1</name>
        <name evidence="9" type="ORF">N0V83_005684</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms a chaperone-bound H2A.Z-H2B complex that acts as a source for SWR1 complex-dependent H2A to H2A.Z histone replacement in chromatin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Forms a heterotrimer with H2A.Z-H2B, stabilizing the association of the histone dimer. Also, with a lower affinity, forms a heterotrimer with H2A-H2B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CHZ1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAJ4"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019098">
        <property type="entry name" value="Histone_chaperone_domain_CHZ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09649">
        <property type="entry name" value="CHZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01082">
        <property type="entry name" value="CHZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone chaperone" evidence="8">
        <location>
            <begin position="46"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="21"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4369920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="100" mass="11019" checksum="1B0BE9EBB24ECF43" modified="2023-11-08" version="1">MEGVQDPATDNKGKGKAPEGDVMEESMDDDSSDESGAEDQVGEVEEPDEDNMEEIETSNIIGSRTRGKNIDFAKANQELGEDEDEDDDDDFVDPDDEMKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBC2</accession>
    <name>A0A9W8YBC2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003391</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBC2"/>
    <dbReference type="OrthoDB" id="5397701at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37331">
        <property type="entry name" value="YALI0F11671P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37331:SF1">
        <property type="entry name" value="YALI0F11671P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="229" mass="24885" checksum="27C74D8271E23AEC" modified="2023-11-08" version="1">MCPIVYQMFANNFVRSARSIRYPRKAPVLRLRGSRNVSTLAENPHIYVHKHPLDPHKSLLSLLPTNPPTPQLAIGTCDSLPVTPQNFTPNPSFLPILSSVFATHATSDPYVVQQASAYASPGGFNLSSQPTDGSGGANHQGGAGGAGHGGWIHVSDVRNPPDYGRIAWPEDILGSVEVDGNGRFSDGTWQDSGTYRIVTREGVLGLTEFMRGKMVERLQELEREIKLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC17</accession>
    <name>A0A9W8YC17_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pre-mRNA-splicing factor SPF27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_003322</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPF27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC17"/>
    <dbReference type="OrthoDB" id="205794at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008409">
        <property type="entry name" value="SPF27"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13296">
        <property type="entry name" value="BCAS2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13296:SF0">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SPF27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05700">
        <property type="entry name" value="BCAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="145"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4373031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="24732" checksum="564975FC3397C1E4" modified="2023-11-08" version="1">MPLITETFDSLPYVDTPLDEQSLAAAKAAINADIRSAGVDTSELHPALIPAAKYTPTFSDFIEREHARLDDDPTSKLSGIDLKRYEDLDAPENTTPTSDEDKPELLARWNRALKQAYTSDEYVQGRLTQLGLLEKFGKNAWLVGNSQLEDILKGIEAELADLRKQQEEVEMLRRSQQENVHGEITTLEETWKKGVGRVLETEVAAEGLKQQILEKRRAGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCM0</accession>
    <name>A0A9W8YCM0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003137</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCM0"/>
    <dbReference type="OrthoDB" id="3792622at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040986775" evidence="2">
        <location>
            <begin position="21"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="273" mass="29462" checksum="86ECE6CC4706BC30" modified="2023-11-08" version="1">MQYRLVSVLATGALIAVSNGLPIADPEAQGFSIGKPADTIGLGPPNTETTWGKRNADFGKPADTITLGEPSTVVTWGKRDEPQPNDIETGAPTTVVTWGKRDIDYGKPKDTIELGQPSTEITWGKRDDEPQPNDISTGEPTTVVTWGKKRGIVFGQPKDSITLGQPSTETTWGKREAEAGKPKDTFNLGKPTTVVTWGKEKRDIVFGSPPDTIEHGQPTTVVTWGKRDQPKDTITLGQPSTETTWSRRDAEAEASEPKDTITLGKPSTVVTWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCZ2</accession>
    <name>A0A9W8YCZ2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003806</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCZ2"/>
    <dbReference type="OrthoDB" id="3940373at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112:SF16">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4372033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="268" mass="31182" checksum="3F32210C541B5404" modified="2023-11-08" version="1">MVTDYNTRALSYETDVLSALAGLANALATTHGDTYFAGIWKEDLQTGLCWYVSGSDYSNSDVQVASNQSSPSWTWIAQRGKEIEFRGWENNHNVANHEGVTFFEHWTNGVFKSEDRFSTIRPKALLLTGRMRKLAVETISKHDYEWKNSLNKTNNDLVAARWVWRVRDIKTNDELGHIAFDFDPVHFETRDVYCLLCIAREKYGEWQLTCLGLVPTDDTLEEFRRIGLIMLRQKDWFGELHVFDPDEETAYIRRPSKDIRFVRTIRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDA7</accession>
    <name>A0A9W8YDA7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Isochorismatase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_003090</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDA7"/>
    <dbReference type="OrthoDB" id="167809at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053152">
        <property type="entry name" value="Hydrolase_YcaC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43559">
        <property type="entry name" value="HYDROLASE YCAC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43559:SF3">
        <property type="entry name" value="HYDROLASE YCAC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040983669" description="Isochorismatase-like domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Isochorismatase-like" evidence="3">
        <location>
            <begin position="34"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4374349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="249" mass="27022" checksum="1D11DAD06F1F5744" modified="2023-11-08" version="1">MKFGFGAALLAFGLSNAVQGDAVPWERIDKNNSLLLILDLQVGLFQTARDWDPSLYKQNIMAHAELGKLFDLPIIMTTSADHGPNGPLPKEMLEMYPDAPLIRREGEVGAWDNADFQAAVKATGKKQIIVAGITTDVCTTFLALALRAEGYSVFANIEGSGTYTELVRDTANARMQQAGVQLVSLFSIVCDLMRDWRNTPGAKEVLPYLDRYLPVYGMLARHHAAAVDNGTAIPGEIELITGANGTIVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDL6</accession>
    <name>A0A9W8YDL6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_004137</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDL6"/>
    <dbReference type="OrthoDB" id="6017at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04369">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000083">
        <property type="entry name" value="WGS project CABT00000000 data, contig 2.8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037382">
        <property type="entry name" value="Rsc/polybromo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054551">
        <property type="entry name" value="RSC4_Ig-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062:SF21">
        <property type="entry name" value="CHROMATIN STRUCTURE-REMODELING COMPLEX SUBUNIT RSC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062">
        <property type="entry name" value="SWI/SNF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22994">
        <property type="entry name" value="RSC4_Ig_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="59"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="258"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="161"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="349"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="481"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="200"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="383"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="418"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4372363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="715" mass="77474" checksum="3C3B327FE456FC28" modified="2023-11-08" version="1">MESAAKRKAANAAVGDSDGRPAKRQKVPAETNANSETVESTTTQGLKFLESLKQAKDKTGRPISSHFLTLPSKGELPDYYVHIKLPIAIDTIEDKLNNGEYTSLAQVESDCKRLVNNAKAYNDKKSIVYEDAERLRKTASNWMVKHNPAYRDGNYVAVATPIPGEDNSAPGKPIPRIASTPRAVHTPVATPDTSERPRRAAAAAQSATPAPSKLRHSASAAPDEDDDNPDYAGKSFQQAQEQIIREIIDYEDGGLQIFLPFQNLPSRSLKDYYQLIKDPMSLTGVQKKVRGVVGRDAPTGHTLFKSWDAFENAMSLIWTNARIYNEDGSDIYNLSLELEEIFHKKLREAKAKVDEPPQPKLKLNMSSGAAAPKQQLKLKLRQSPGSDRNTPARNSATPGVIVDGDALQRQQRHVLDSMNGNNRSSRPTSAGKAGTPSVSSNPFTGPSGGAATISALPSRTAASPALNGVKQDVQSPALSAIRPASTRSDSQDQRLSVPAQTPLPVMAPPHTIARPASGSPLPNGLSQQPSAYSAPYQQPNYYAPPAAPRVDNFRKVPLKNIDEALIPKITLNTHPALNLAKPWSVDVFANKQKTAHSATIFVSPTNSYLQITPKVPIALTSRLYRLFVTVNGNRTLEVNRVPVTAGINGSSPGPGYEGGKKKGEPVFEAKLVGGVNRIEVEIVAEKEHKGGNSEKDQIETEKCTIFLHLVRQSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDM4</accession>
    <name>A0A9W8YDM4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Complex III subunit 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">QCR9</name>
        <name evidence="13" type="ORF">N0V83_002183</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The complex plays an important role in the uptake of multiple carbon sources present in different host niches.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein, 2 core protein subunits, and additional low-molecular weight protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the UQCR10/QCR9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDM4"/>
    <dbReference type="OrthoDB" id="44067at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.260:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.260">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008027">
        <property type="entry name" value="QCR9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036656">
        <property type="entry name" value="QCR9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12980:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12980">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX, SUBUNIT X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05365">
        <property type="entry name" value="UCR_UQCRX_QCR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81514">
        <property type="entry name" value="Subunit X (non-heme 7 kDa protein) of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4375100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="66" mass="7583" checksum="5D1AAA96B9ED8DC0" modified="2023-11-08" version="1">MSSALSSVYNAVIRRNSTLLFTVFGAAFGMQLAFDTSSDKVWDAFNRGRQWKDIKQRYLEKAEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDM6</accession>
    <name>A0A9W8YDM6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Malic acid transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_001957</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDM6"/>
    <dbReference type="OrthoDB" id="2901184at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015140">
        <property type="term" value="F:malate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09317">
        <property type="entry name" value="TDT_Mae1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.150">
        <property type="entry name" value="Voltage-dependent anion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030185">
        <property type="entry name" value="Mae1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004695">
        <property type="entry name" value="SLAC1/Mae1/Ssu1/TehA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038665">
        <property type="entry name" value="Voltage-dep_anion_channel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31162:SF0">
        <property type="entry name" value="MALIC ACID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31162">
        <property type="entry name" value="MALIC ACID TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03595">
        <property type="entry name" value="SLAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="392"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4374879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="417" mass="46041" checksum="648DE9E4DAEE5F95" modified="2023-11-08" version="1">MSRPLSAVTADGTLPLQGDSAKHTKQLSLKQRLRHFTWSWFECTMSTGALATLLGQQPYAFHGLKTIGKIFFLLDIILFLVFCACITYRFASNRASLSSSLHHPHESFFFGSFFVSIALIIYCIEQYGVPSSGPWLIKTLEVLFWSYAGIVMLVAVFQYHVIFDQEELPVTDAMPAWILPVYPFLILGVLGSTLLKSQPVTSGLPIFIGSVAFQGLGWTIAFFMLTLYFTRLVNSELPEASKRPGMYVAVGPAAYTANTFVALGMQAPKHVPHNFLGITSFPVGDLFKAFGVIAGIFLWLVAFWFSALTTVSVVTSAKSSHFTLNYWAYIFPNAGLTIALIQIAKVLDSDGIKGVCSGATIILVILWFWVAVMNVIALRKKQVLWPGMDEDMEDIEGHSQDPSEDGEGSQEDNNSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDU1</accession>
    <name>A0A9W8YDU1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA mismatch repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MSH6</name>
        <name evidence="10" type="ORF">N0V83_003034</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Component of the post-replicative DNA mismatch repair system (MMR).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDU1"/>
    <dbReference type="OrthoDB" id="10252754at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032301">
        <property type="term" value="C:MutSalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000014">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.110:FF:000006">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.10:FF:000002">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000771">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003810">
        <property type="entry name" value="PRK05399.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF148">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="9">
        <location>
            <begin position="1060"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="130"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="203"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="259"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="214"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR037677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4374293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1225" mass="136076" checksum="6295EE9B30A279BD" modified="2023-11-08" version="1">MVRGLEGNEMSTPRPSQKKQPASGSQSGKNQKSILGFFQKKSTNSPSPAPSEPSTVKKTPTSTLTKRAFTKPPASITPVPSSDAPELSSPIKQEQDSTVGRNKENGLLSPITPVDAGTNRVLSEVAGVAFSSPSRKARKSVNYAESDDDDDDDVLGPIANNARGRATKRRRISVEDDSDDEFGLDAATQEALLDDELDDFIVADDSEDEDVVSKKRKRPASTKASRKASPASSPPAMVTKEETEEDVVMTYTSTAQQWTYDPENPMTPKPRPATLATSKDGHKKAKARPSASEPETRYTWLANMQDADRHPPEHPDYDPRTLYIPPAAWKEFSPFETQYWEIKSKFWDTIVFFKKGKFYELYEKDATVGHQLFDLKLTDRVNMRMVGVPEASLDMWATQFVAKNYKVARVDQMESALAKEMRERDGKTDKGKGQKKADKVIRRELAAVLTSGTLVDTGMLQDDMSTYCMAIKEIDRDNLPAFGVAFVDTATAQFQLCEFTDDIDMTKFETLIAQMRPGELLLEKSCISAKALRILKNNTAPTTLWNALKPGKEFWPADITIREVEANNYFESPTEDNIEAWPPVLREAREQDLVMSAFGALLQYLRTLKIERDLVTLGNFQWYDPIRKATSLVLDGQSLINLEIFANTFDGSGEGTLFAMLNRCITPFGKRLLRQWVCHPLADAKKINARLDAVDALNADSTIMDNFSSSLSKLPDLERLISRVHAGRCRAQDFLKVLEGFEQIEYTISLLEQFGGGEGVIGQLIASMPDLATSLSKWKSAFDRELAKKEGILVPEPGIEEDFDNSQEEIDNCKANLEVLLKKARKELGSNTICYRDNGKEIYQLEVPKKIKVPSSWDQMSATAKVTRYYSPELRKLVRALQEAQETHGQITREVASRFCKRFDEDYSIWLAAVKIIAQLDCLISLAKASASLGEPSCRPVFEEGNRTVIEFQELRHPCMLNTVDDFIPNDVRLGGDSANISLLTGANAAGKSTILRMTCIAVILAQVGCYLPCTSARITPVDRIMSRLGANDNIFAAQSTFFVELSETQKILSEATPRSLVILDELGRGTSSYDGVAVAQAVLHDISTRVGCVGFFATHYRSLAKEFECHPEVQNKRMRIHVDEDSKSITFLYKLEDGVAEGSFGMHCAAMCGIPDKVIKNAEKAAREWEHTSRLGERMEVKKEEGGVYVPLGVQSDVAWALREGLEGVGDRALDVLLEAIAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDV0</accession>
    <name>A0A9W8YDV0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_002004</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDV0"/>
    <dbReference type="OrthoDB" id="7722975at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009166">
        <property type="term" value="P:nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.780.10">
        <property type="entry name" value="5'-Nucleotidase, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036907">
        <property type="entry name" value="5'-Nucleotdase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006179">
        <property type="entry name" value="5_nucleotidase/apyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053828">
        <property type="entry name" value="Nucleosidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014485">
        <property type="entry name" value="Pesterase_C1039"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575">
        <property type="entry name" value="5'-NUCLEOTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575:SF22">
        <property type="entry name" value="ADL392WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21953">
        <property type="entry name" value="NadN_nucleosid_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017316">
        <property type="entry name" value="Pesterase_C1039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55816">
        <property type="entry name" value="5'-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040963512" description="Calcineurin-like phosphoesterase domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="63"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Putative 5'-nucleotidase C-terminal" evidence="3">
        <location>
            <begin position="357"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4374926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="619" mass="68312" checksum="9AB571621012652D" modified="2023-11-08" version="1">MRLLTFGAIAATIATGSACEGDHSCYGPLKDDVVLTRNVRRMQPDAQNATTSPKGPLEWGQINFLHTTDTHGWLEGHIKEQNYGADWGDYVSFTKHMKQKAKKLGVDLLLIDTGDLHDGAGLSDATTPNGNISNAIFENVDYDLLTIGNHELYVTEIAYETFSNFSKVYGNKYLTSNVQIKNPVSQQFEYIGAKYRYFTTEQGLRIMAFGVLFDFTGNSNVSKVIKASDMVQQSWFNEAVNFTKPVDLFLLIGHNPVRPTVSSSTMGTVFKAIRSVKPDVPIQVFGGHTHIRDFVVYDNKATGLESDWNRPTFSYHAEGSQPYTSFDTSKGVQITKDITTDRYKLNLTSLYGCAPETYCQFCKPFLAEGNIFKLLQTALATVVVNETRKDTARLIIINTGSVRFDLAKGPFTYDDSFIVSPFDDAFQFIPDVPYEQASKVLGILNAGAFQKKRRDLSTADFGFSNILADRDTCIDPPLTHDYAGVTRRSHQAGRLIRRQSTSATTPGYTTTDDFGTDGDDTIHSKIPNYNQPNDLQANASFPVDGSDPTTVDLVFLDFIASYIVNALNTPDVGGSYALSDVSYYMDKSFTTNSYLPAYAKIAWQDGVPNCPVGAGIGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDZ1</accession>
    <name>A0A9W8YDZ1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cupin type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002065</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDZ1"/>
    <dbReference type="OrthoDB" id="1161823at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02223">
        <property type="entry name" value="cupin_Bh2720-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000601">
        <property type="entry name" value="Mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052538">
        <property type="entry name" value="Flavonoid_dioxygenase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43346">
        <property type="entry name" value="LIGAND BINDING DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G14370)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43346:SF1">
        <property type="entry name" value="QUERCETIN 2,3-DIOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="2">
        <location>
            <begin position="50"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="168" mass="18453" checksum="1624F41B04820A9E" modified="2023-11-08" version="1">MENYKKAGNRKDEPPKHEMVHFPGLMSEKRSFGDFRTVLHTGLYSQIVAMEVPVGGEIGDEVHTVDQILLFTSGRGLATVAGKDQEVNAGDVVVVPAGTQHQFVTRGDQPLELVTVYSPAEHLPTSVHKTKEEGDKAEEDEIDEAPEWALKPKGENEKSGLVKESGKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE56</accession>
    <name>A0A9W8YE56_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DOMON domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_001847</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE56"/>
    <dbReference type="OrthoDB" id="19261at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09630">
        <property type="entry name" value="CDH_like_cytochrome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1210">
        <property type="entry name" value="Cellobiose dehydrogenase, cytochrome domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015920">
        <property type="entry name" value="Cellobiose_DH-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47797:SF1">
        <property type="entry name" value="CYTOCHROME B561 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47797">
        <property type="entry name" value="DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G05805)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16010">
        <property type="entry name" value="CDH-cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00664">
        <property type="entry name" value="DoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49344">
        <property type="entry name" value="CBD9-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040718444" description="DOMON domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="220"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="251"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="345"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="DOMON" evidence="4">
        <location>
            <begin position="68"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="375"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="406"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="436"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4374771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="476" mass="50715" checksum="0DD3E3FDCDFC096A" modified="2023-11-08" version="1">MVGLTKHFGAVVALGAFFPALISAQNTTAATTAASTFYLDETQTQFSVNIANDSSDVFIYFTSPAYSWVSVGFGDKMADSLMLVMYPNNNGDNVTISPRYGSGHTEPAFTPNVAIDILPGTTVNDSMFVLNARCRNCRVWPNGFLDPKDTAQPMIYAFGPGSSLYSDSQSAGLKRHVRYGFFTMDMVAATGTGRVPSQSSAISGVTMVGEMVKDHDRASFAHAVVGCLALFVVWPLNVLVAGFFKNIKIHVGVSVLVMALLIVAYALGGVASAQFIRSKSFNSPHQILAFLSLLPILLLSLLPIPSLSTLHAKIPRLHGPLTALAFLLLVLTGGLGLGLASQTRAIILIYTAIALIVCVFIVTVQSCIRRRGSAYARSQGGPRRGSGGDEDDDRNYMLGKMEESSRSTSSASLGGAPPPPPAYGMPYGGQFGADGGQYQYQQSQQQQRSPYGGGTMPGPQYLLNMHPGVPVQVSRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YED9</accession>
    <name>A0A9W8YED9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dihydroorotase</fullName>
            <ecNumber evidence="5">3.5.2.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">URA4</name>
        <name evidence="5" type="ORF">N0V83_002400</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YED9"/>
    <dbReference type="OrthoDB" id="1670005at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004721">
        <property type="entry name" value="DHOdimr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00856">
        <property type="entry name" value="pyrC_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43137">
        <property type="entry name" value="DIHYDROOROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43137:SF1">
        <property type="entry name" value="DIHYDROOROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001237">
        <property type="entry name" value="DHOdimr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00482">
        <property type="entry name" value="DIHYDROOROTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4375314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="346" mass="37497" checksum="CFF4FA5042527E2F" modified="2023-11-08" version="1">MEFTKLSEIELPAAFDAHVHLRDGEMSQLVTPTIRKGGVNQVYVMPNLVPPITTVQQCLKYRDRLRAIEPDVDYLMSLYLHESITPEVIREAKKAGITGVKSYPAGVTTNSSSGVLDYKSFYPVFAEMEKQNMILNLHGEVPSTPSHAHAHSSSSSSATNPAITILNAEPAFLPTFLSLHSTFPNLRIILEHCSTAAALTAVLSCGPSVAGTLTAHHLSLTIDDWAGDPFSFCKPVAKTPEDRDALIQAVVHSKGKFFLGTDSAPHERSKKRGEDKVAAGVFTQPHALGYVLDALERGVERGVIGGGGYAGGVGGVFRGWGGGFMGWGWEGERVVLKKGRRRLMMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEG0</accession>
    <name>A0A9W8YEG0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PMT1_2</name>
        <name evidence="7" type="ORF">N0V83_002847</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEG0"/>
    <dbReference type="OrthoDB" id="4161238at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF50">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 3" id="KW-0862">Zinc</keyword>
    <keyword evidence="1 3" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="394"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="456"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="304"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="517"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="626"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="539"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="669"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4374108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="695" mass="77863" checksum="603D9FD3D66CB485" modified="2023-11-08" version="1">MTSATALNKLMASLTWSNINLTPKCTKNAAYDLVLASQELLEELMNESGVVFAPSLLDAVQSAMHPALSDFKSLSVLPGKWWAVYLLVLEKPGSRPKIYIGSGTNRYGGVSERLRMYDSESVLPMYVKRALDNGYAITHRGFLCWAPLPKEGKIYLYRLLFLALESMFSLVFWAMVSRTKDYGLPHLCPWDIESLDYDGCCTHTALMEGIVGAEDGLTLEQIAAKETECRKWRVDQITRLAKNCKRRAIEDRTFACNICSILCNAQWQLDRHLATKKHMKRASGVTKPSNVVAWEHKNVVAKRFPCYICSRFFGSQQKLTTHLASQRHQDEAAASSDSKQVPANEAIDEILVIWWSSTALIGLYIAIKGLAILRWQRGYRDYSNVTFKRFDYEVGMSILGWAFHYFPFYLMARQLFLHHYLPALYFAILALCQMFDFVSYRFSVFGLKQYPAIGQAAAVGFLAVAITVFTVYAPLAYGNAWTKDACNTAKLFGSWDWDCNNFLTSYEAYNSQTTPVASISAPSSAPPPPVAPQIPRQQEQPKEAKPEEVVSEQAAVSPPPQDVPKDLPVLSKEERIEYRDENGRVLNDDEVQALAGKVSFKTRYETRTRIIDGQGNEIYEGLVDAHGAAEEQGVAPPHPDVEGRNPETQQLDQAAEASEAPPTVEVVEDQQKEKSVEQHKNEAKPASEAQAATQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEQ5</accession>
    <name>A0A9W8YEQ5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_002984</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEQ5"/>
    <dbReference type="OrthoDB" id="3357846at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015244">
        <property type="term" value="F:fluconazole transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF23">
        <property type="entry name" value="FLUCONAZOLE RESISTANCE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="261"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="413"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="440"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="472"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="507"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="100"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="27"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4374243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="541" mass="59134" checksum="5AB7FF3F81651806" modified="2023-11-08" version="1">MSYLIKHSTVGTIVHYAKHRNFDFSRTPNKSISSSSSDDDLPFPKEEVPGGNDNGNDNDNDNGGAIGAELKEYQTSLVDWSGPDDPSNPQNWPMSHKVLMSLVICTYTFAVYVGSSIYSSSQAAVIEIFGVSEVTGSLGLALYVLGYGLGCLLFSPLSEIPAIGRNPPYAISGFFFVILCIPTALADNFAGLMVLRFLLGFMCSPCLATAGASLADIWTMAQFPFAVALWAATATLGPALGPTLSSYAVKALGWRFSSWELMIIAGPFYLMMVTLMPESSGPTILYYKAKRLREETGNQNLMSDSERKQHNLHVGSLLFDALIKPWEINALDPAILFTTVYMGLCYGAYYSFFESLPLVYPVMYHFNAESTSLIFLAIAPACVLAFTTHVLYLKYRVFPRLTNGTFGELENHLLPGLVASPLIAIGLFLFAWTARPSVHWIAPTIGLGLTVFGIYFIVQSVFLYIPNIYPKYAASIFSANSLSRSLFAFAAILYARPMFQGIGIDGGVSLMAGCMVLCAGGMFLLFWFGKRLRARSRFAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEU9</accession>
    <name>A0A9W8YEU9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphorylcholine phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEU9"/>
    <dbReference type="OrthoDB" id="5182398at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050582">
        <property type="entry name" value="HAD-like_SerB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040749487" description="Phosphorylcholine phosphatase" evidence="1">
        <location>
            <begin position="22"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="358" mass="40144" checksum="477DE602EFFAF381" modified="2023-11-08" version="1">MRSSIITSALWLASAHHLTSATPSYGAPTVQFKHWPADAAAALSAMIIKNANQSNYAVFDMDQTSYRYDLEESLLPFLENQGILTRDNLDPSLKLIDFKDTANFTETLYSYYNRICEVDDLVCYPWIAQVWSGFTLRELKGYVDDLLAHESTVPTQYWDGENITQSNVNPPKVYPGQVELYNALMSNGIAVYVVSAAHEELVRFVASDPKYGYNVPPQNVIGVTTMLKNASSGALTNARKQIIEGTYDEAANLDLIVTTYLWTPATWFAGKWAAILTYIDQWKRPVLAAGDTPGSDSYMLFHGVDTRKGGVHLWVNRKEEYLDVIKAEIKTNVEGQKENGREITADKNWVFVRPADIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEZ0</accession>
    <name>A0A9W8YEZ0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_003685</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEZ0"/>
    <dbReference type="OrthoDB" id="4161332at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051615">
        <property type="entry name" value="Transcr_Regulatory_Elem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31313">
        <property type="entry name" value="TY1 ENHANCER ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31313:SF86">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="7">
        <location>
            <begin position="44"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4373390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="202" mass="23374" checksum="1C22590F5E273BC9" modified="2023-11-08" version="1">MSEEWVEKGLVAEAARQRQLENVNYHLGKLDFDGVEPKLGMHLLSLHWNRQHHSFLITHRPAFMRDMASNGPYFSKLLLNAIYFSASKFSHRHELRKEVNDVRTAGWQFRERVRELLGGALDRSDITTIQALLVMTNSLFALGDERSAAWLYAGLAFRMIVDLGMHVDTPHLADNRKFSDEDIEIRRRVFWAAFGKSNIHVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGA7</accession>
    <name>A0A9W8YGA7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_000385</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Sodium-phosphate symporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGA7"/>
    <dbReference type="OrthoDB" id="260807at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101:SF57">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="474"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="517"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="565"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="592" mass="63939" checksum="9F5DD1C4E690AAD7" modified="2023-11-08" version="1">MPRLEQYNYVFAIGTFFALLDAYNNGANDVANSWATSVSSRSVSYRQAMILGTIFEMVGAITVGARTAETIKNGIIPTTAFKGDAGVQMLAFTCALAGASTWVMWCTKHSAHVSSSYSLISSVAGVGVAVAGASNVQWGWNKGKGLGAIFAGLGMAPIASGCFGAIIFMLIKLTVHLRRNPIPWAIWTAPFFFLIAGTICTLSIVYKGSPKLGLNKKPAWYVASVTVSCGVGLALLAFLFFVPYLHAKVVKRDHTVRWYHVFQGPLLFRRPAAEGAEHAVVPNYAVVQHDEEMKPESISDSDNGVVSHDNEKAAVVAEHRQLSYQELVAQGQERFHAKLRTKRGPLGWAMRYLHANKIQTGEIYEKKNMLITLKRIPAMIVVGSLYGINYDIHAAQTGIHGTPEGKRMERVYSHAKKYSNEVEHTYSFVQVLTACTASFAHGANDIGNAVGPWAVIYSAWNTGNAAASKAPVPIWQLAVLSATLSLGLITYGYNIMKVMGNKITYHSPSRGCSMEMGAALTVLIFSQYSLPVSTSMCITGATVGVGLCNGTLKAVNWQRVGLLGFSWIMTIPIAGTIGGLAMGIILNAPHFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGD9</accession>
    <name>A0A9W8YGD9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_000267</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGD9"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044283">
        <property type="term" value="P:small molecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00682">
        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="3">
        <location>
            <begin position="214"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4377442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="391" mass="43665" checksum="D3118C520047D012" modified="2023-11-08" version="1">MATDTRDAPRLPDATTYEEARNGAAAYLQSRLAAAAKHDVTDNEFEVPVIDISPSFSPSLEDRQAVAARIQKACTTSGFFYITGHGVPQNVRDGILQQAERFMHELSMDKKEALHLKKNKFGLGWEPSNYTSIAGDEEQKEVFNFAYEAELDPTGGDGLYRNLDGTKYKGNMWPKDEDLPGFYDAVKEYYGAVLGLARHLFRLFALSLNLPETYFDSMTTHPGGIARLLYYPPPKNPQPVSPSSEELIGLGAHSDYECFTLLLASSTPGLEILKPSGRWHVASYIPGAFIINVADFLMRWTNGRYKSTVHRVVNRGTEARYSVPFFFSVNYDTVIETLPTCLKEGEESTWKPIRAGEYILERLNATTQYGMGFQEQDVDPKLGGQSGTHLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGI5</accession>
    <name>A0A9W8YGI5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF1750-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_002189</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGI5"/>
    <dbReference type="OrthoDB" id="5321006at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013859">
        <property type="entry name" value="Ssr4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046464">
        <property type="entry name" value="SWI-SNF_Ssr4_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20497">
        <property type="entry name" value="SWI-SNF_Ssr4_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08549">
        <property type="entry name" value="SWI-SNF_Ssr4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SWI/SNF and RSC complexes subunit Ssr4 N-terminal" evidence="2">
        <location>
            <begin position="12"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="SWI/SNF and RSC complexes subunit Ssr4 C-terminal" evidence="3">
        <location>
            <begin position="262"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="SWI/SNF and RSC complexes subunit Ssr4 C-terminal" evidence="3">
        <location>
            <begin position="416"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="458"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="493"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="506"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="580"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4375105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="675" mass="73493" checksum="0899AFD5B042C480" modified="2023-11-08" version="1">MYGMNQAYNVPDPSAQVPNHLLAHVHLISSYRFPTLPSLQPAQALEYLIKGPQIVRDTSPVAWTYFASPPQDGTVILTWQPPRMQTHFASDGMVWADAETAYDMNVRGYTLQVLVHNSGYTYPHEPFALHARFRYRIAAGPGTTDPNLWLVHYRPAEAQHRIPAGQIPIPREVHQQLQMRAQLQAAGPLMRKEFMLVDQANWPKVEFGQQAMRGQQPYYNPMQPGRPYTAQPPPAKRPRLPTQPQARQPVPGSATVPDTSLEEEENATQDAFDFLTPREISLSRYKQHHEWMEEVFSSPYDVGKIAPIDLGLGLMGELAPLTAGILDISGAENPEGAKDGYKIKNYYKLDPEQLKDFEKRVAEYTKKEQAEIDKMKAEHARKMAQLKRTRTYIKAERRLRDLSRSTDSDVDGVNPAEAVVQDLEKSLGVTFESQKSVVCVEKGGFIEAQQPSPPKAQVNGNGTQQSTNGASNGVNNDGTLEENSAASLLDQYGNGSLSGTPVGNISLPRLSQPPSQSQSAAGTPGAPTGNINQNSTFDSGDGNLDVDVGDDLLDLDVEMGGMPDEGGKPDDWVMVGDQAGDTSQSASHAQQPSTSNNPTSNAGAMPSSTVDAEAGSMFDNADFGSFDNLDSAGDALADYGNDDNLGLDLVDDSAFGDAFHGTEMHHDETGDGDNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGP0</accession>
    <name>A0A9W8YGP0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Chromatin structure-remodeling complex subunit RSC7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">NPL6</name>
        <name evidence="4" type="ORF">N0V83_000467</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGP0"/>
    <dbReference type="OrthoDB" id="5598844at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013933">
        <property type="entry name" value="CRC_Rsc7/Swp82"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22597:SF3">
        <property type="entry name" value="CHROMATIN STRUCTURE-REMODELING COMPLEX SUBUNIT RSC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22597">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08624">
        <property type="entry name" value="CRC_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="460"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4377639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="490" mass="54600" checksum="244FB97EA2CD505B" modified="2023-11-08" version="1">MARGRPSRAAARRSTPVRSATPQQDTDEDMIDAPESRAETPKDEEEEDAESAAQVSDEEQAPSERSPSPVVIQQFPRKKRLGRPPKNRPPDWNVIEPDNSEGGTPIKRKRGRPSGGGRGRPPKGGPSHVTRVPIDKEGNMMDVVNDEVDLPEDDEGEEKVDKLGNLQGGRDYRVRIFTIRGRGERQYMLSTEPARCCGFRDSYLFFTKHMKLYKIIIDDNEKRDLIDREIIPHSYKGRAIGVVTARSVFREFGARIIIGGKRVIDDYYVTAAKESGAVEGELADPNDRLPPPGEPYNKNQYVAWHGASSVYHTGVPSVPTANGKPVPGKRKVNITSANWQFEHGRAASRFNSNLSALRKANLTGVYDPHTNLLCYPKVTQPTHARWEEVSSAATDVPPLVRRRYLIVDTFFQQPPFAGLGIPGPDGDYIDVGSNGLPNLDDEDEQNMKPEELEAFTQLKREEQEWRSQWGGESTDGARAKPRIGFVGVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHX4</accession>
    <name>A0A9W8YHX4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000041</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHX4"/>
    <dbReference type="OrthoDB" id="48988at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="90" mass="9324" checksum="B33693CE92BB6CEA" modified="2023-11-08" version="1">MNASVATVRDAAAKHSISGHAAAVRWTAFHSVLDGKYGDAVIFGVSKIEQLHQTLDALEAGPLPAELATVISAVYASIEAVEGAAPPYHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIE1</accession>
    <name>A0A9W8YIE1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Nonsense-mediated mRNA decay protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NMD5</name>
        <name evidence="9" type="ORF">N0V83_000909</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIE1"/>
    <dbReference type="OrthoDB" id="760868at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000244">
        <property type="entry name" value="Nonsense-mediated mRNA decay protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013713">
        <property type="entry name" value="XPO2_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF18">
        <property type="entry name" value="D-IMPORTIN 7_RANBP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08506">
        <property type="entry name" value="Cse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="8">
        <location>
            <begin position="24"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="7">
        <location>
            <begin position="781"/>
            <end position="1031"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4378078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1046" mass="119247" checksum="9AFAFB6F68496881" modified="2023-11-08" version="1">MDVAGLRDRIQATLDPNAAIRQQAELDLKHAEEKPGFTDGLLNILEVEQDHAVRLSTVVYLKNRVSKGWSPAEEYSQAKPIPEEEKASFRNRFVPVLVAAQPQVRIQLIPTLQKILAYDFPSRWPNFLDITIQLLNAGDIASVFAGVQCLLAICKIYRFKSGENRADFDKIVAMTFPQLLNIGTSSASETSLEAGEILRTVLKVYKHAIYFDLPASLREQQTMVGWCTLFLTVVGKEPPESSLPEDLDERETNHWWKAKKWSYANLNRLYVRYGNPSALGKNNEVDYTEVAKNFIANFAPEILKIYLQQVEKWVGKQIWLSKASLYYTLNFLDECIKPKSMWTLLKPHVDNLISHLIFPVLCQSDEDIELFEEEPQEYLHRKLNFYEDVTAPDVAATNFLVTLTKSRRKQTFSVLNFVNEIVNRYEAAPDNEKNPREKEGALRMIGTLSGVILGKKSPIADQVEYFFVRHVFPEFRSPHGFLRARACDTLEKFEQLDFKDPNNLIIIYRNILESMADPTLPVRVSAALSLQPLIRHDIIRTNMKQNIPQVMQQLLKLANEVDVDALANVMEDFVEVFAPELTPFAVALSEQLRDTYLRIVRELVSKNQEKGDDSEYGDFLDEKSITALGVLQTIGTLILTLESTPDVLLHLETILMPVITITLENKLYDLYNEVFEIIDSCTFAAKSISATMWQAFELIHRTFKAGAELYLEDMLPALENFVNYGSQTLVQNRPYLDAIVDMVRTIFKDDKVGGVDRICGCKLAEIIMLNMREHVDDFVPEFISLTMQVLTNDEPKVKSLRIHLMEVVINSIYYNPALALHVLETNGWTNKFFSLWFSSIDNFTRVHDKKLCISAICALLTLRAQDVPISVQQGWPRLLQGIVRLFQTLPAALKNREEAKKEDSFDFAAEYGEDDEDEEWEQEADWNNEADEAEDVKDESAAYLEFLNEEAQKFSSVDDEDDDELEEESLLETPLDKVEPYSMFKHALLRLQQEQPALYDNLTKNLNPEEQQVVQGAVHQADVIAQSQAQAQAAAANGNQALAPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIH0</accession>
    <name>A0A9W8YIH0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Multifunctional tryptophan biosynthesis protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="16">Anthranilate synthase component 2</fullName>
                <shortName evidence="16">AS</shortName>
                <ecNumber evidence="16">4.1.3.27</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="16">Anthranilate synthase, glutamine amidotransferase component</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="16">Indole-3-glycerol phosphate synthase</fullName>
                <shortName evidence="16">IGPS</shortName>
                <ecNumber evidence="16">4.1.1.48</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="16">N-(5'-phosphoribosyl)anthranilate isomerase</fullName>
                <shortName evidence="16">PRAI</shortName>
                <ecNumber evidence="16">5.3.1.24</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="21" type="primary">TRP3_1</name>
        <name evidence="21" type="ORF">N0V83_001501</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 16">Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 16">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21540"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="EC" id="5.3.1.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6 16">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 16">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 16">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="16"/>
    <dbReference type="EC" id="4.1.1.48" evidence="16"/>
    <dbReference type="EC" id="5.3.1.24" evidence="16"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIH0"/>
    <dbReference type="OrthoDB" id="524799at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004425">
        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000136">
        <property type="entry name" value="Multifunctional tryptophan biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000031">
        <property type="entry name" value="Multifunctional tryptophan biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00134_B">
        <property type="entry name" value="IGPS_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016302">
        <property type="entry name" value="Anthranilate_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001377">
        <property type="entry name" value="PRK00278.2-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF4">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001382">
        <property type="entry name" value="TrpG-trpC-trpF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00614">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 16" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8 16" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="12 16" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13 16" id="KW-0456">Lyase</keyword>
    <keyword evidence="14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 16" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="18">
        <location>
            <begin position="27"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Indole-3-glycerol phosphate synthase" evidence="19">
        <location>
            <begin position="259"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="N-(5'phosphoribosyl) anthranilate isomerase (PRAI)" evidence="20">
        <location>
            <begin position="581"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR001382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ4376218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="777" mass="84450" checksum="992B7809569DC5A2" modified="2023-11-08" version="1">MPSLELIDHSPRHPDPSPQIPTASNVVLIDNYDSFTWNVYQYLVFEGANVTVYRNDEISLDDLVAKKPTQLVVSPGPGHPERDAGISNEAIKHFSGRIPILGVCMGEQCIFYTYGGTVDVTGQILHGKTSPLKHDAKGVFAGVSQNVPVTRYHSLAGTHGTLPDCLEVTATIPANEDADVKEVIMGVRHKQYVIEGVQFHPESILTEDGRIMMRNFLRMQGGTWAENERLQKEAHNDAIGAATNGTANGVKKNKQTSILEKIYDHRKAAVAEQKKIPSQRPSDLQASYDLNIAPPQINFPERLRQSPFRLSLMAEIKRASPSKGIISLSTCAPAQARKYAKAGASTISVLTEPEWFKGSIDDLKAVRQSLEGMPNRPAVLRKEFIFDEYQILEARLAGADTVLLIVKMLDQGLLKKLYEYSRSLGMEPLVEVQNVEETEIAVKLGAQVIGVNNRNLTNFEVDMETTNRLISLVPKETILCALSGIAGPKDVEPYVQSGVGAVLVGEALMRASDTAQFIAELLGGTSTRVTQGSSTPMVKICGTRSAEAAKAAVEAGADLIGMILALGTKRTVSAETALAISEVVHNTRKPQISRSGLLAESKYATDFFEHGSSRLVSSEARALLVGVFRNQSLEYVLEQQRLLNLDIVQFHGQEPLEWAKLVPVPVLKAFNPHDHGIGSRGYHALPLLDAGSGGSGQQLDLTDVKGVLGKDDGVKIILAGGLSPENVQNVLFGLGEYRDRVKAVDVSSGVEVDGQQNLDKIRAFVRAAKSQENMFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJ99</accession>
    <name>A0A9W8YJ99_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJ99"/>
    <dbReference type="OrthoDB" id="416217at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022698">
        <property type="entry name" value="OrsD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052400">
        <property type="entry name" value="Zn2-C6_fungal_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47657:SF3">
        <property type="entry name" value="ORSELLINIC ACID_F9775 BIOSYNTHESIS CLUSTER PROTEIN D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47657">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN ECM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12013">
        <property type="entry name" value="OrsD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="465" mass="52087" checksum="E25E7870A37A6CFF" modified="2023-11-08" version="1">MSPPIEGLPIIEGYRCTISGCNHLCASEKRMRRHWNENHEDIKHPFLEENLRKARLQTFFRGTKIRYFEVDSSATIDGANIASLVIATENDDGFKNESHMDVEYTETSHPQSSTMSHITTAPAAPCRVPPVDLNLDTLLYFHQFITQTSLTLPMLNRAESTVHYWQTEVVSLALRQRWLMCGVLALSAFHLAVFSDGMATEGAHRERAAAFCVEFTKEWEKATKRDMGLVDKETEIAGQHLQRLLRITSLTSKGVMQRISPESTGYSRLQVLIADIGSLVPSGSVPDPDGIRYAHDDTPTEGVNHSTTSKSDQDTLARHLNRLRALPARLTAALGPPEPDHLEEVLHTLTAIAVLIECSETSFASEDALENNTDNQARDWETVSRAAWEGMSMWLTKVSPSFYESIWRDNPASLVVLAHWALLVGRAERRGYWFLSGAAAMIVDEVKDRLEKEDEAMQSLIGDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJD4</accession>
    <name>A0A9W8YJD4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nephrocystin 3-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_000182</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJD4"/>
    <dbReference type="OrthoDB" id="195446at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF15">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="716"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="746"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="9">
        <location>
            <begin position="32"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="439"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="527"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="788"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="793"/>
            <end position="810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4377357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="810" mass="92420" checksum="4928DB52857EE088" modified="2023-11-08" version="1">MNKVEAAEAYRWLSPGDHFAKHTHIKSSRAPGTLQWLVDSPQFQRWLDHEQQVLLCTGKPGSGKTVATSLVVDTLFEKFGFNLGIGIAFLYHAYGQEDQNPERFLRSVLYQLGHRLRLIPTALIDLYRRYNNSASSPSVQELLGIIFETISKMSKVYLVIDALDELKSSHRRVLLSGLLKLQENLHVSLFATSRGFPDVVNRFQQFPSLEISGSLNLSYATLIFKHKFLHAKVLLDELATAQSPKSLSWKLSGLPVSPELHELYAEMMSRIERMVKEDRTLAKTALAWLLFARRPLKEAAFQHALGIQKGLSELDNDKVPDTIQIVALSAEYLWATQNDWWPEAKAMIAKYCLTYLAFDDIGQGYCKTDEEFEARLRENPLYEYAAQYWVDHLRDTPDLPERAITSFLIDQWKVASASQAMQWSEHESREQGYSQKIDHKQTGLHLAAQFGLESVIDLLLVAGQRPAVRDSKGLTPLWRATEESHEAVIRLLSRVDRTTFTVMLAEQEFSLAYSLLRHAGQTVKDTRLRTALHLGVIHNAPELIQNALKYGVDINAKDGDAYSPLQLAIQYQYDLSGKDQWMTLDYLSTLQSGSMPDSGMAFFNDLRPDVLNANGKQTTLINRLLDDANTWITLRHQLRKQAETARTFRKHYLKFADGDEAVGLLDRTIETFADRIALRIDKFDAASSALIQLEFNLTSIREAQKSTMLNRSIKRLTWITFVFLPLIFVSGLFGMNVNVLAGNPPWWWYVVFMLGTLCLTMAVWVAFKRFPELEDNLEDKFGWLVKPRRPKGQSKDVEKGWVADRKEKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJU2</accession>
    <name>A0A9W8YJU2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJU2"/>
    <dbReference type="OrthoDB" id="3595585at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="548"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="325"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="457"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="513"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="548"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="570" mass="64055" checksum="1526030A958EA91A" modified="2023-11-08" version="1">MPGAQASNPTEPQDENKTVRLHITPLRPELLKVYLAPSVLPSAQNISYHTVETFPEKGFGYVDLPEAEAQKLKKKLNGTTLRGSKVRIEMAKSEKRKAREEADAAKENAEADRPSKRVKKEKRKKDLGVLDGVELPDDRKVKRGWTEPPSKNKKERKEKKDKKDKDKAREQKHSKYTKEPELLFKAKLTPVAAAEVAHKEKSKEKKEKKEKNKSKSREMVIHEFEKNTKTPSFLKETKVTTTKKPAVDYVNGVGWVDEDGTVVEPETGRAKHRRALELVDKPTEAQKAWIDGLALPQSSTSETKKSKKEKKDTPPPSSEEDDESSVVSSSSEDASSSESEAESEAESAVSSPQPDANGTRTPEKQQALPSEEKDVHPLEALFKRPKSTPDATSTPAKKLTPLNTSFSFFDSNEENTEVNGEPVENVPTTPFTEKDLEWRGLRSAAPTPDTAAIGRRFSFDWRKGSQEAEDDEEMEDVDPDAEQQLSQNTKANAAAKHLPGVTEEDENEDAEAGAEQQATGADEQPESEFRKWFWENRGDLNRAWKKRRRDALKVKRHTENKRMTGGRRVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YK94</accession>
    <name>A0A9W8YK94_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YK94"/>
    <dbReference type="OrthoDB" id="5234213at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="153" mass="16246" checksum="FF4804045A350C2F" modified="2023-11-08" version="1">MSSKAHSKTVRGTLDNPKTQEAIKSDKPTETLGDATSLKPENKDKSLPSKDSAPPRGQGNDKDSKSAPTVSESYRKGKDAGGADKKAKGNGREEKEDLPHSKTVRGTLAHDEGKKVNKTQVGDPASLKAETSKSEMGKGTEREGEDKSGKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YKA5</accession>
    <name>A0A9W8YKA5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Topoisomerase 1-associated factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TOF1_2</name>
        <name evidence="2" type="ORF">N0V83_000890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YKA5"/>
    <dbReference type="OrthoDB" id="310853at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="11"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4378060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="268" mass="31250" checksum="6A72B4D3FC6067C8" modified="2023-11-08" version="1">MTLESIDLIRKFEFDPPTYEDGKAPEELLRSKAAAARRSTRRVDFDDDSDGIDHDSEQDRGEYGPDAATARKPDGERKKLKRRKRARTPVELDEDEKDRRAEARRKKEIEKQTKVMLSTMFIHDSDDEDWDADKDATFFAREQALRDEAMTAFQKAMTLGSTEPASSKKRKADEPAKKSKRRKSPPKRNAPFDDSDDEPDEEMEDAVSINSRASSRSSRRYLGRWKRRRGYHRHSTVISARRYRSCERRTKSTKITYYHYEGPGRYNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YKT9</accession>
    <name>A0A9W8YKT9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Zinc finger Mcm10/DnaG-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_000955</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCM10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YKT9"/>
    <dbReference type="OrthoDB" id="202825at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000174">
        <property type="entry name" value="DNA replication licensing factor mcm10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040184">
        <property type="entry name" value="Mcm10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055065">
        <property type="entry name" value="OB_MCM10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015408">
        <property type="entry name" value="Znf_Mcm10/DnaG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13454">
        <property type="entry name" value="PROTEIN MCM10 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13454:SF11">
        <property type="entry name" value="PROTEIN MCM10 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22379">
        <property type="entry name" value="OB_MCM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09329">
        <property type="entry name" value="zf-primase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MCM10 OB-fold" evidence="10">
        <location>
            <begin position="321"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger Mcm10/DnaG-type" evidence="9">
        <location>
            <begin position="472"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="80"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="685"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="173"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="227"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="249"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="270"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="703"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4378122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="762" mass="83863" checksum="D37690CBAA0A4589" modified="2023-11-08" version="1">MIPNWPPQSPVEALLSSPSGRRKYERQRERNSVSPSPSKRRPIPRGGIPAEEDEEDEDEETLQLKLQAIEAKLKLKALQNKAKKTAGSGENDSNASSSRPGTAAELRRVDLPRPKADIQVPHSPVRARRAPDEQKSPARVLLGIDKGLTARDVSLKRPGSGMNGGLSRTNSTRRAETPKIKSFSERIAESRNKEKEREEIETKLETRRSRGFGLHKIEGLKDRPAVSRSNSSISTATKSSVEMLPPTSKGLSRAQSRSTNDLHNPPTPRPGSNLSARSESTQPPGTTSRPSSGFGNTPTASKYAEISQRDNSSEASSFESFSGLHLKTREMQHNVVTRTLEGKTVFTIPQLLKIVKAPDYDPPDWENDYVVLGVICSKSTPLNSKNAARDQTKSTQERGVDQNGKFMVVRLTDLKWELDLFLFDTGFSQFWKLPIGTLVAILNPDIMPPRQRDTGKFSLKLTSSDDTILELGTARDLDFCHAQRKDGKECGQWVDSRKTEYCEFHLQLQVEKSKRGRMEVNTMTGFGNGPRGGKFGMFGGGRGGPKGDELKREGRYHDRELHETVYIAPRPGGAATLIDRDEQSWERGASREERFRKQLAEKEKEKDLAKKLGTMGDGTTGGDYMKIKGAGTPNLPARGYMSSQDSALKPDKPADIDFTGLLGRTSADVSLAPVKRKRIASGKSAASSEPVGWGFKYGRIPSPKKDTSSALRGTRETSPAKKKARLLLPEKGIREPGRDSLGGLDVGLIAAMDEDDDDLEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHF4</accession>
    <name>A0A9W9CHF4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_009968</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHF4"/>
    <dbReference type="OrthoDB" id="10257314at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000003">
        <property type="entry name" value="Alpha-ketoglutarate-dependent taurine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051323">
        <property type="entry name" value="AtsK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468:SF28">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT TAURINE DIOXYGENASE (AFU_ORTHOLOGUE AFUA_8G02210)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="8">
        <location>
            <begin position="90"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="17"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4363672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="376" mass="41902" checksum="2508427D6D696601" modified="2023-11-08" version="1">MQANGQAVSILAQALSERSVPESVPQVGDVKEDTKEQPSEPAKDKPKYPHYLPVWNYGEKYLPWEPFTHTEHNKDADASFKDLLVEGTKIQHLTPTTGSEVIGVQLSKLSDAGKDQLALLVAQRKVVAFRDQDLADLSIQDALDLGGYFGRHHIHPASGNPEGYPEIHLVHRNGSSSEFESFLANRNSSVSWHSDVTHEEQPPGTTFLIILDTPDVGGDTAFVNHVEAYNRLSPAIKERLHGLTAVHTGFEQAEFSRSRGGVVRREPAKHSHPLVRTHPATGEKALFVNGGFTPTIDGLKKEESEYLLKFLLDHISRGIDYQARVRWAPKTVVVWDNRVTSHSAIVDWVTGERRHLARITPQAERLYETPYVAEAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHM6</accession>
    <name>A0A9W9CHM6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHM6"/>
    <dbReference type="OrthoDB" id="5428890at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="363" mass="41646" checksum="1659C886DBE32CFF" modified="2023-11-08" version="1">MASPAASLLDALHPSTKALISNAFWGPHPTWALFHRDADQFEAYWKFYHRECERALHDGGRHVLARTHQDILDVVTALEQDKSRENIQLTLRAKLTKTHDNEDELVDRSIDLAASLWLMVDFGNTQYGFCGRRQLQWSGESFKNCVASGFDSTPWLGHKGVKLQRVFNALNLDRIAGVEILPTSNLLDHLRLTDDDTKLYIFHHASVLKCQTHKYVNKHPKFTRWLLTPTSTAFPDGLIAETIHTLALLFPQSDAGVSDWSRQLPNFDEFDPHLLLCGHLKTDDRQIENFTYWHDRLVVLKQVFDEATPRTFSQWWHDRRNGVQWYTFWVAIVVLCLTMAFGLIQSVEGALQVYGTFRSGTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHN2</accession>
    <name>A0A9W9CHN2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein DOM34 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">DOM34</name>
        <name evidence="13" type="ORF">N0V83_009552</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the Dom34-Hbs1 complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway (PubMed:20890290). In the Dom34-Hbs1 complex, dom34 recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following ribosome-binding, the Dom34-Hbs1 complex promotes the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHN2"/>
    <dbReference type="OrthoDB" id="10249111at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990533">
        <property type="term" value="C:Dom34-Hbs1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.870:FF:000004">
        <property type="entry name" value="Protein DOM34 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000004">
        <property type="entry name" value="Protein DOM34 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000008">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="entry name" value="Pelota, domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058547">
        <property type="entry name" value="Pelota_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="TF_pelota"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PELOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853:SF0">
        <property type="entry name" value="PROTEIN PELOTA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26356">
        <property type="entry name" value="Pelota_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="Dom34/Pelota N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0469">Meiosis</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="12">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4364097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="407" mass="44902" checksum="A1187A37C650E259" modified="2023-11-08" version="1">MRLLKSQIEKKTGAGFATLLPEEPEDMWHAYNLIQESDRLRAKAVRRVSKTSDAGSTSSQRVTLDLTIAVTSTEFDIGSGQLHVSGRVASENEHVKMGQHHTLDLELNRSFTLEKADGWDSIALEMLKEACDTGKRAELWAVVLGEGTANICIITEHQTILRQKVEVSVPRKRKGGVDGHSKGMDKFFSTTLSTLLRQMDLPNMSAQQDKTLPLLLASPGFVASAFLAYIKAEATRTTNRPLLMLIRSIIIAHSSSAHVHSLNEVLASPAVTTKLSDTRYARETAYMDKFRTLMREDDGRAWYGPREVERAVDKGAVGRGGGVLLINNALFRAQNVAQRRRWVKLVDRVRDTEGGEVRVLSSQHESGKRLEALGNVAAILTFPLEDLDEDDDDPVGGEDGEGGEMII</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHP8</accession>
    <name>A0A9W9CHP8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009597</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHP8"/>
    <dbReference type="OrthoDB" id="3778454at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="223" mass="24948" checksum="7CFB18F2F96A7BA8" modified="2023-11-08" version="1">MLDIQSRVSNLERSSSTIGEKIGDGAPYDKTKYASSESQTWWEACQNFARNCDTPFSANEFLRTPRRFSGFDFHFDAFKSRPRTPPLTPEEEADGMQNNTPSASFNPKPRGSNEHSNDNVLERVPEFDKTRILTPPNLQTPPGSVHNKSLSVLSVEADITALPELPTEPAPPLVAERSQRSYKGIKRMFTYKALLKSKGSEEEHPTFLHFNRKASGSKKSVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHS2</accession>
    <name>A0A9W9CHS2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Lon protease homolog, mitochondrial</fullName>
            <ecNumber evidence="11">3.4.21.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 18" type="primary">PIM1</name>
        <name evidence="18" type="ORF">N0V83_010055</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial DNA in a site-specific manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homohexamer or homoheptamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12 13">Belongs to the peptidase S16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="11"/>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHS2"/>
    <dbReference type="OrthoDB" id="2411602at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141164">
        <property type="term" value="P:mitochondrial protein quality control"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070407">
        <property type="term" value="P:oxidation-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19500">
        <property type="entry name" value="RecA-like_Lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000021">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000113">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.5270:FF:000001">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1480:FF:000002">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000015">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03120">
        <property type="entry name" value="lonm_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054594">
        <property type="entry name" value="Lon_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027503">
        <property type="entry name" value="Lonm_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008268">
        <property type="entry name" value="Peptidase_S16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718">
        <property type="entry name" value="LON PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718:SF2">
        <property type="entry name" value="LON PROTEASE HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22667">
        <property type="entry name" value="Lon_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01046">
        <property type="entry name" value="LON_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 11 12" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="17">
        <location>
            <begin position="159"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="16">
        <location>
            <begin position="865"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="65"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="264"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="793"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1067"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="389"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="91"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="276"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="798"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="814"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="1082"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="active site" evidence="11 12">
        <location>
            <position position="957"/>
        </location>
    </feature>
    <feature type="active site" evidence="11 12">
        <location>
            <position position="1000"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="576"/>
            <end position="583"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4362938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1092" mass="120497" checksum="CFFDB9B1BD0EE08A" modified="2023-11-08" version="1">MLHGQNLPWRAAVRHASRSTPRLAAFRASSRFLPAQCTASLVARPLRQCTSPPAALCAYRGFATSPSRRKEKEPKTPPEAVEESKEEETEDGKKPKEQDKVEELEGKSNAPEPIASKPSSARGSAAGGDGSGGDGGGKKRKSAERSLVKPSIPDVYPQVMAIPLVKRPLFPGFYKAITIKDREVGQAIADMVKRGQPYIGAFMFKDDAADKDVIHDPSEVYDVGTFCQVTSAFPVGADDNFAMTCVLYPHRRIKMTGLTSPKAEKDEASVVESTLDESSATDASATQSKGDVVASFEEASDEAKSSQEGQVPTILKGRKVSIADVDNMVEEPFDLKANGTIQALVNEIVNTFKGVALLNPLFRDHVSTFSVHTTMNVGEDPVKLADFAAAVAQAESHELQSALEEMNIEKRLSKALEVLKKELISAELQKKVADDVNARVTKKHREYMLMEQMKGIKRELGIESDGKDKLIEKFHEKASKLAMPEAVRKVFEEEMSKLQGLEPNGSEFNVTRNYLDWLTQLPWGLRSAENFGIKHAREVLDEDHHGLKDVKDRILEFIAVGKLRGTVEGKILCLVGPPGVGKTSIGKSIARALNRQYYRFSVGGMYDVAEIKGHRRTYVGALPGRIIQALKKCQTENPLVLIDEVDKIGRNSNHGDPASALLELLDPEQNNSFLDHYLDVPVDLSKVLFVCTANMDETIPQPLLDRMEVIRLSGYVSDEKIAIAEKYLSPAAKDMSGLKDADVILEGDAIVELINKYCRESGVRNLKKHIEKVYRKAALKIVQDVGEEALPEAEALTEEGKAAQQESEKDNSDLNETPQDISKQTTEKPRVALKVPDSVHVSVTKDNLKDYVGPPIFTSDRLYDFTPPGVAMGLAWTSMGGSALYIESIIQNSLSATSHPGLERTGSLRDVMKESTGVAYSFAKSILAREYPKNKFFEHARIHLHCPEGATPKDGPSAGITMATSMLSLALDTKIRDDIAMTGELTLTGKVLRIGGLREKTVAARRAGAKMVIFPHDNMNDWLELPENIKEGIEGRPVSWYKDVFDIVFHDLDKDYASKLWDKELKSKKGDRKKREQKRKEEEEEESGEDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIN8</accession>
    <name>A0A9W9CIN8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cullin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CDC53</name>
        <name evidence="14" type="ORF">N0V83_009154</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11 12">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIN8"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000014">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000007">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000011">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000026">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.130:FF:000003">
        <property type="entry name" value="Cullin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000029">
        <property type="entry name" value="Cullin homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016157">
        <property type="entry name" value="Cullin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01256">
        <property type="entry name" value="CULLIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="14" id="KW-0436">Ligase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="13">
        <location>
            <begin position="405"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4364558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="769" mass="88864" checksum="80836EDC734CAABC" modified="2023-11-08" version="1">MASTMPPAPAKDDVNATWRYLEAGVDKIMTNLRGGMDMKTYMGLYTAIHNFCTAQKAVAGSSFHAANNRGGAHLLGEDLYQHLIEYLKKHLSGVQAESKQHVDEALLTFYIKEWNRYTTAGQYNNHLFRYLNRHWVKREMDEGKKHIYDIYTLHLVRWKEDMFTGTQESVMRSVLKLVEKQRNGETIEQSHIKSVVDSFVSLGLDEADSSKSTLDVYKEHFEKPFLEATAQYYDNESKQFLAENSVVEYMKKAEARLEEEKERVPLYLLNEIMSPLMRTCEQSLITNHSQALREEFQILLNHDQQDDLGRMYKLLARIPEGLDPLRTQFETHVRKAGLTAVDKLSQEGDNLEPKVYVEALLEVHTQYQDLVNKAFNGESEFVRSLDNACREFVNRNKICKSGSNKSPELLAKYTDTLLKRSSAKMSEEDDMERLLTQIMTVFKYIEDKDVFQKFYSRMLAKRLVQTTSASDDAETSMISKLKEACGFEYTNKLQRMFQDMQISKDLNTAYKEWQSNNLDETDLKTNVDASYHILGTGFWPLNPPTTPFTPPQLIVQTYDRFARFYNHKHQGRKLTWLWQLCKGEVKANYCKVAQLKTSPTFQVSTYQMAIMLLFNDSETVSYDEIAEATKLNKETLDPSLGVFIKAKVLLAQPEGAKAESGTTYKLNTGFKTKKVKMNLNIGIKSEQKAEAEDTHKTIEEDRKLLMQSAIVRIMKSRKKMKHQQLVSETIQQIKNRFMPRVPDIKKCIDILLEKEYLERLEGDELGYLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJA9</accession>
    <name>A0A9W9CJA9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJA9"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4365556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="126" mass="14843" checksum="E94480B5216F4CC7" modified="2023-11-08" version="1">MDTTLAEDWHESASQLSHERMVTTQAEPMVTTHAEDEHEVSEERTHIVFRYSILWKAITSNAEDYPSLVDKEVIQLLCGPHFRSAVTEGDLRLNTKFPTYWQRKEILRKAFDSIFRLLSYRVRDPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKP0</accession>
    <name>A0A9W9CKP0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetylxylan esterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_005856</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKP0"/>
    <dbReference type="OrthoDB" id="2586582at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000675">
        <property type="entry name" value="Cutinase/axe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33630:SF10">
        <property type="entry name" value="ACETYLXYLAN ESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33630">
        <property type="entry name" value="CUTINASE RV1984C-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01083">
        <property type="entry name" value="Cutinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01110">
        <property type="entry name" value="Cutinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040777749" description="Acetylxylan esterase" evidence="3">
        <location>
            <begin position="17"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4368774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="22910" checksum="19F90DB054DAFFB0" modified="2023-11-08" version="1">MKVTAAAALFAGLAAAGPVELAERQSCPKVYIFGARETTLPQSNGYGTAGGLVNSVKSAYPGAGSEAIVYPACGGGSSCGGVSYENSASQGTAAVVKAVTAYNQKCPDTQIVLIGYSQGGQIMDNAICGGSGSTLTGAALAAVKAAIFMGDPHNVNGLSYNVGTCKAGGFAARPSGYKCAPANPSIIQSYCDSADPYCCNGNDANAHQQYVNKYGSQALTFIKSKLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKY2</accession>
    <name>A0A9W9CKY2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_006126</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKY2"/>
    <dbReference type="OrthoDB" id="3819888at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052178">
        <property type="entry name" value="Sec_Metab_Biosynth_SDR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618">
        <property type="entry name" value="7-ALPHA-HYDROXYSTEROID DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618:SF4">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE_REDUCTASE FAMILY (AFU_ORTHOLOGUE AFUA_7G04540)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="226"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="234"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4369044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="293" mass="31381" checksum="0A8525F68736ADD7" modified="2023-11-08" version="1">MSSKSNNSELTLGAVFDVKDKVALVTGGGSGIGLMITQTLAVNGAKVYIVGRTEEKLEKVVEVHGKDIAGQIIPIVGDVSTKDGVQKLVKEIESREKCLCVLVNNAGIAPGKAETKAESAEELTKNLFEPTSEKEWLDVYQTNVVGPFLMSTAFLPLLQNATELHKGYSGTIVNISSISGHIKISQGHFSYNSSKGATIHLNKLLASEIAKNGIKVRVNSIAPGVFPSEMTTNDSRDDNQKSEMPKEHKEHLPSQRPGKDQDMAAAILFVVGCQYLNGQTITVDGGYAIQVGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLP7</accession>
    <name>A0A9W9CLP7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="6">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">UBP6</name>
        <name evidence="10" type="ORF">N0V83_006038</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLP7"/>
    <dbReference type="OrthoDB" id="333239at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061136">
        <property type="term" value="P:regulation of proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02657">
        <property type="entry name" value="Peptidase_C19A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16104">
        <property type="entry name" value="Ubl_USP14_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044635">
        <property type="entry name" value="UBP14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982:SF1">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="107"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="391"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="508"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="423"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4368956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="584" mass="65645" checksum="55A49510D189F65C" modified="2023-11-08" version="1">MFVVIVKHQGKKHEVEVDTTSNGETFKYQLFSITGVEPERQKILVKGGQLKDDADMSKLGLKPNQTIMMMGTPSGDGVVIEKPKEKIRFLEDMDEAEAAQLEGATPAGLQNLGNTCYMNSTLQTLRSIPELQEELLRYSTSNDAGSSSSARQLSQFGLSGLGASTDLTGSLRDLFKQMSETQQGFPPLMFLNALRTAFPQFAQKSKDGHGYAQQDAEEAWSQIVSQLRQKLKIKENETPSDADKQKELSWIDKYMCGKFESVMECDEPAAKEGGEEPVNSEDLFFKLNCHINVETNHLRDGLAAGLKEQIEKRSEVLGRNALYTKTSKIARLPQHLPVHFVRFDWRKDTNKKAKIMRKVTFPEELDALEFCTDALRKQLVPIRDKIRDIRKEEQDDERARKRQKRMKAGEENDVETPASSKEPLQKKKEAEQKKEDAKKPAEDVKMEEIEYKTDAQLDAERAASILAAKKELLALVDQELAADEGANQTGLYELRGVITHQGASADSGHYTSFVKKQGPKDPVTGKRKEEDGKWWWFNDEKVSEVDSERIQTLSGGGQSHSALILLYRAVPLPTVDEDVKMNDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM39</accession>
    <name>A0A9W9CM39_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short chain dehydrogenase/reductase dmxR8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">DMXR8</name>
        <name evidence="2" type="ORF">N0V83_005524</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM39"/>
    <dbReference type="OrthoDB" id="542013at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF31">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="325" mass="35240" checksum="44CFD7EB079E25AA" modified="2023-11-08" version="1">MGFFYSQLFVRLPYPSGSYVGKTVIITGSNVGLGKEAARHFVRMGASKMILAVRSLDKGTAAKHDIESTTSCAKDLIQVWKLDMASYASVQKFSARVEAELDRVDIFIANAGIAPGKYATAEDNESSITVNVVSTLLLAALMLPKLKATAARFGTRPTFTIVSSDVHGHTTLPQKSAPEGKLLATINDKITAEKHWDEQYPISKLLEVFAVRSIAEKYGPSKYPVTINAVNPGLCHSELGRDFPTVGFWLIKLILARSTEVGSRTLVQAASEGVKSHGQYMSDCAIGTPAPFVTSKEGKQAQGRVWDELVKKLETIKPGVTSNFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CML6</accession>
    <name>A0A9W9CML6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005094</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CML6"/>
    <dbReference type="OrthoDB" id="440781at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16105">
        <property type="entry name" value="Ubl_ASPSCR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17075">
        <property type="entry name" value="UBX1_UBXN9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021569">
        <property type="entry name" value="TUG-UBL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059238">
        <property type="entry name" value="UBX1_UBXN9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46467">
        <property type="entry name" value="TETHER CONTAINING UBX DOMAIN FOR GLUT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46467:SF1">
        <property type="entry name" value="TETHER CONTAINING UBX DOMAIN FOR GLUT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11470">
        <property type="entry name" value="TUG-UBL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="388"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="490"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="208"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="254"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="284"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="491"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="530"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="541" mass="59025" checksum="D70DFB49F4FE298F" modified="2023-11-08" version="1">MSHVVVFNSYARTVKIPTTPVKYLTDVRDEACQKLGVNKDQYTLKYNNKPISLSQQVRLANLPQGARLELVQASRSPTVISVALQLPASEKNVRLTQKFASNTSLWEILRQFESSEGANYNFTQRGVPEMSGTSGAGRLHYEMPVITVMPGHKEQSSFVELQKTLSQLGFDSGSALLKLSYKNSGQPLEEAMAQISQYFKAEEPESTGAEGAHAAAAAQEASIPSLDQAAPEATETVAGETIQNGGQDPDPMEVDSPSVPESVPEPEVTMQNPASQNIENVSPAAALAAATSTASTELVDDSTSSSPPADKPRNVQIFSAPTSSTPQAARNAFNESDYLPTIEHAKSHQAALLNKTRNTRLLSDKELEEQEKARQEKIEAAAAKGGALRIRMPDGTLIQMDFTKADTASALYTFVRSFLEKKDEPFKLNYTSPTGRLVLIPQDDNKRLIQDLRFFNNELITFQWAENASTEARASRKTLASEWQAKATTLKVDEPVAREQPRASSSQGQTVAEGKRKAAMSSEEKESKLKSLLGKGLFKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMU4</accession>
    <name>A0A9W9CMU4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMU4"/>
    <dbReference type="OrthoDB" id="438641at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053209">
        <property type="entry name" value="Gramillin-biosynth_MTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47643">
        <property type="entry name" value="TPR DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G12710)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47643:SF2">
        <property type="entry name" value="TPR DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G12710)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="350"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="726" mass="81950" checksum="743384134430AE96" modified="2023-11-08" version="1">MARPDPIRNTYEDFPDQLKELLKEGKILEEHTLPRVPRDALLHDHAWHLKLLVEAALPPRNLVLPPPYPPSIVSTSEGKQKNISDLRVQARDPNEFVVLHTITDPYVFSSTVTIVEDESGNAARLTVCNLEDSILDPIISKGSVLVVKQPCWSRLPERGCHVRVDHPSDLVVLDQSDKAVPKIWRKDGALDTSKNATTWKKEGDMMFLKKRFRKALELYNEAHANISESPDTVGQIDVLRKRCGVNLVLLRFDAAARDLAQAVSLYAKHDTTLSSSELSDSSVVEAWLHDHSTKDPMKIAVTLPRPLKDLAARIKFDLEIYQTTVDYDLPRMSSYVGPLTLHVDAANYISDTEIKQTPHHGRGLFAARAFRSGDLISAEKAFALPGYFYNDRSTECSLYSLGDGTATDRAGALLFKELVQKLRHNPSLRKGFFDLDDGGYWKEHGWELKDGDEIPVDVFRIEHIRRRNCFSAPVRSLDLLTSPSVPIRNGFWNHTSYINHDCLPNSVRTFIGDILFLRATRDIAQGEEITTQYVAPELVFEDRQQKFMGSWGFTCECTLCKVDQTIGGKLEAERMTIFEELKGMAQKVGTKPTVTALKKFARRLRDLEALYNDETYAQLPRLCLVHPTLFLTEAWRSLKNVDRMIESANKLLRNFGIVTGVESDSFEVVRSSGMVNIETVRALKYMAEGNESKGKGTLAEQIRTVARVWFRIITGADVGVEEFLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMV9</accession>
    <name>A0A9W9CMV9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_005141</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMV9"/>
    <dbReference type="OrthoDB" id="655030at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051704">
        <property type="entry name" value="FAD_aromatic-hydroxylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46865:SF2">
        <property type="entry name" value="MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46865">
        <property type="entry name" value="OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="4">
        <location>
            <begin position="4"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4370620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="424" mass="46787" checksum="CB73B89D53B90D83" modified="2023-11-08" version="1">MRTMHVLISGAGIAGPALAWWLARNGIAQVTIIEKAQSILPHGHNVDIGGSARTVIKKMGLRDKVLELNTTEKGTQFIDPKGRPYAAFPVQQGSAASLTSEWEIMRGDLAKLLSEEAKKCPGVEFLFGTTISSVLSNDSTSVKVLLSNGETRSYDLLVAADGQWSKVRKQCFPPEAVTVVDKDMYVGYWTIPRLPSDNEWWNIYHGLGSKMISTRPDPHGTIRAMMSQMPLSPAQKRAWQEASKADRQSQQDLLRKEFGAAGWQADRLLEEMDAAPDFYFQAVQQIKMAKWSEGRVVCLGDTAYAPTPLTGMGTSLALLGAYVLAGELGKLPPNGESQHPAVALDAYEKVFRPFVEEVQVIPSWIPGIAHPETAWKRWVFGVVMGVISWLASWRWLVSRFGKDDDAEDFVLPVYLEPEVGAGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNE2</accession>
    <name>A0A9W9CNE2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003144</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNE2"/>
    <dbReference type="OrthoDB" id="5372859at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4372853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="283" mass="31879" checksum="17BB9281D485BBB2" modified="2023-11-08" version="1">MATPDLISHFQFLVSPRFPPELVLKTIQHLPFGNGKVIANLRSTHSRLRDILDNYEHSITRCFVGKELRHAPTDFPREEAFSLSWLAHTVDEHSAKLAYIKSLQCDPLTAIYLALHHATLSARYHGSGWIHQRTYGRFMDANQLSLRNELEFCFAEAALSDGPEFINGMLLHHNTSNAETTLLNCYHDHGTHDWDWPCWGTGPGEFEPPRTQGPHKEAGSTSRSLFTTLLEREAELIDCPFGEVRGRIEQFTDAKDHALAYLDLAGKARLMQGLDLDYGGGNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNJ4</accession>
    <name>A0A9W9CNJ4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003492</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNJ4"/>
    <dbReference type="OrthoDB" id="2534600at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4373199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="359" mass="40867" checksum="4D5559A266AD3401" modified="2023-11-08" version="1">MSENGAEQLHLELIAPVPTIHQIINDWFDWIHPVAPILHRNLADHLALWSSASKITLERTLTLYNLSSAVHHEYGIDSSLTHRFSGEASVGVKYLLHQELDEMPFVDQQILKRLYWLVFAGQCTCDMHGRHLLVLRHAHEPIRSLLPMEIPDEQLLLGREPSPTSSQGGNHSYVPGLNALSRLFLVWQTSQAVPYQTMENLQEHISRAQQVLNDLPPELSYTGQASQVGDFGFNVQKVNLKITQLHIRSNLLEQMNTLAKDQHLRITPNAIIEERHRVVDELLDLLYRMPTEVFDANGYSIVPKIRDIGSALLDELRTGSHGRTLQASINLDRLLVKLEELDLRPIVDCEVVNYHDLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNQ6</accession>
    <name>A0A9W9CNQ6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNQ6"/>
    <dbReference type="OrthoDB" id="2384430at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010972">
        <property type="term" value="P:negative regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052945">
        <property type="entry name" value="Mitotic_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43628">
        <property type="entry name" value="ACTIVATOR OF C KINASE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43628:SF11">
        <property type="entry name" value="PROTEIN DSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="696"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="225"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="284"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="490"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="711"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="726"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="741" mass="81818" checksum="444D41C6E3710F7C" modified="2023-11-08" version="1">MVNSPNRPGGLEFRSQSFEPPRNVGIGQLASASMSSLSLADLPSPRAGEVPPALSPLDALALQGRLLARKFEQQDTKTGRRLSRLAPLTIQNEFGNRSGFFSSLSSSALNSPNEEAGPILPRPESSPRQEVSEKHKSFYPQFGGDELNYGNPLHMRSHLAPIGEASPATPPLQGYFDIPRSHSPEPAEPHIGSLHEATPVSPPVSAPSSRLFLSPQRKQSFDSQPRSQPRSNLLPPRSSSSLHSQRSPRLSPSIRSVPEDSSDEVEDMSLSGSYDSLQHNRNLSPSSSFSRAHSPFTPVTGSSIPRSPSVASEYSTNGSNLPRPSYNFSRPMSKHSIRPSLDVKASMDGPSRQVSEESVYMRPSFDSQPRRKDSYDSPTTNYASETVHTPVSMVSEDFRQSGDFTSNPAPSYTYAKFSLPRGRKVDRRSMGIEDFFKSQITWDQPNHNQARPPSPDSPPRSFDQTRSFDQSRKHHDYCPARQASFDQGAPSLTSSNSTIRGRPTGSAEITAQEHCDMGIELHEAGELLKSTYHLRLAARAGLPEAMFWYGLACRHGWGMRENKAEALQWLRRAVDSGQLEVADDEDQSRHGQPTDLLKKKQHRAQYAVAIYELGKCYGNGWGAAQDKALALRCYEIAGNWGDADALVEAGYCYAEGVGCKKDMKKAAKLYRTAEAKGVSMVGNSWIYKEKYMDDNNPTTSDARSVRSGRSTKKDHSEKKARDKSRTRIIFGRKKSHTVTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNS9</accession>
    <name>A0A9W9CNS9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SNF-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_004098</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNS9"/>
    <dbReference type="OrthoDB" id="6581954at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11554">
        <property type="entry name" value="SLC6sbd_u2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF240">
        <property type="entry name" value="BLOATED TUBULES, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="294"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="351"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="391"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="422"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="457"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="484"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="556"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="586"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4372324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="663" mass="73069" checksum="285C27505CD0A547" modified="2023-11-08" version="1">MAAFIKKVGGWVAPPPQKSCDDGRDQWPSRAAFLLAAMGGCAGQGNLLRYPSVLYNNYGLQWFIPYLLAVFLIAIPALILEVSIGQAYRGGTVIAFNNINRRLKGVGMGPVIVSFIVVQYFTVNLAWIMNYFRWSFTSPLPWENRIEDFYWNDVLKVGNITEGALTPDGNSVAAYQGYPSRAVLGETVGWSIFIWFLIWVSIFRGVGLTGRVVYFTMGLPIVTTIIFVSRALSLPNASEGVALVWRTWRGDQLASGTVWQTAVGQVFFSTGIGFGYFTSYASYNQRHSNAVMDAVLICGSNVLFENFAAFAVFGVVGYLRRWPEDGVRLGAFVVGFLTLPEAVLQMPGANFWAVLLFFTLVVLGFSSAFVMLDVVVTLICDSGLVKASRPVVVTVLTVISFLMCIPYCTEFGYYLLDGIDRWVNNVALIFVVWSEVSSATTVYRWRDIADQTGLPAFILYNVGFFGGQLFGISLAHGITNPGAGAGAGIGFWAVCSIVGTIIARTPDAKAPSFWNGNPFLRKFWFLAFYSGNQLRRDLNVIVGQNGNWKIPSFLPILLRYLSGPVLAIIFSFAFPEFHTLRYDPMMIAGFILSILTIIAVLLGFVMPRYYDAFIPVGRRGEGTEPTIAMEPKHELAGRPSADMVYAEGGLVREGSSEHEKSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-06-18" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP02</accession>
    <name>A0A9W9CP02_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ATP-dependent permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">HST6</name>
        <name evidence="12" type="ORF">N0V83_002765</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000604">
        <property type="entry name" value="ABC transporter B family member 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001471">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF15">
        <property type="entry name" value="ALPHA-FACTOR-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="92"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="847"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="887"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="967"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="992"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1070"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="48"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="376"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="850"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="1168"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="613"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="709"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="764"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="716"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="736"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="764"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4374026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1480" mass="163360" checksum="2B641FC56D8F189C" modified="2023-11-08" version="1">MATEIVSNDIDDHEKTIADSIEERDNVSQVGWKALFSFTTRKHLPVLAGALLGAVIAAATLPAFAIIYGLIFRAYTDYGAGKIDSNALLSSVTKYCIILTGIATLNWVANSFYFFLFLTFGELQARSARDKVFDVFMKKDMIWYDTRETGIAAFLPAVQMHIRDLQLSVSAPFGEGVQCIVQGLAALVVAFYYSWNLTLVIISTVPLIYIVQAFISHRLSIPALAQAKKLQHALKYITSAIQNIETVKCLNGERHELQSFTKIASQAACFYRRVANLRSLQIGVMQFFTLSVFFQGFWYGSHLVASGNRNAGQVLTTFWAALMAIEGITGFLPQFIVLQKGKMAGSRLCVLMKKITTSDDQGQRGQLKPTRCIGDIEFRKVTFSYPTRTDEFAVRDISLFFPAGETTFVIGKSGSGKSTLGQLLVRFYQPSSGEIFLDGHALDDLDVQWLREHITLVEQHSVLFNDTIRENIAMGRPGKSVDLQEVQEAIKFAMLDTIIQDLPDGLETHLGMKGSSLSGGQKQRVALARARIRDAPILILDESTSALDYITRSAILQAIREWRKGKTTLVITHDISQIQSDDFVYLLDNAELVQEGYRKNLEAQTGAFQSFLASHPGNEESNDGDFSDAGNGTDEVITLYGEPWNFHSPMHRPLSAVLFGESMLQPFLAGRRESWVDNVTVGSAKRLSLQGYEGLVYNHEGVSLKVPPSALEAHPNRSSSTPGIFQPEKNGSSLNRQASQHSLSSLASRRNRPINTLMVFGSRPSSVYSSRPVSRQADYPRRLSVSSTSPVQLEPPKKRPRHQKLLSSVGLSRRQRLVELPTPHTDSLPLMEILKSVWPALNWRTRLILFAAIFSAALHSAATPLFAWVFAQLLATFYNPGDQTPRALVYALSILGIAVADGIAYYLLFFLSDSVAQSWTLALKTEAMRRILMQPREFFDKEQNSISRLAETLDHFAEEARNLPGRFACIFLVVILMITISIAWSLVTAWKLALVALATGPILYAITKCYNMISSRWEQLSNEADDEVGQVLHETFVNIRTVRCLILEDYFRGKFRSQTIAALKVGIKRALYSGAIFGLNYAAVLFVAILLFWYGAVLVSSDQYTVTDITETFLILMLSINHVAHVSNYMTQVNMSRAAGSRLLRLARLPITSHELSGTLHIQKAGDIVLNNVNFTYPTRKDYPVLQNISFRIPRGSCTAIVGSSGSGKSTIASLLLKLYQVDTNVFSTSNDREMTISKINIKNLDTTTLRSRMAIVSQNPVLFSGTIAENIAYALSPSSPLASIDSIRAAAEAAGISEFIDSLPQGYQTIIGEGGTGLSGGQAQRIAIARALVRDPDILILDEATSALDVASAAVVRATILKLVREAKPVRPPLSPIPSSPITPITPMSPRAGGFWDDKDWEAEAVQGRAIGEQKAKTVGKAPRGEMTVIIITHAREMMAIAEHIIMLDKGRVVEQGSFRELKRKRGGAFGRLLRGEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP37</accession>
    <name>A0A9W9CP37_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP37"/>
    <dbReference type="OrthoDB" id="4510061at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="82" mass="9137" checksum="6D07D453C75CA13E" modified="2023-11-08" version="1">MSDGLNDARAIRVAEIMTDFRNLQHYLVQLRATPTAEEYYLEGYSLLRQCASEAQTILQTPFSGSSGAATGEPEREKQQLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPD8</accession>
    <name>A0A9W9CPD8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_003539</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPD8"/>
    <dbReference type="OrthoDB" id="5982228at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001317">
        <property type="entry name" value="Putative ADP-ribosylation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
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        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF353">
        <property type="entry name" value="METHIONINE TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
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        <property type="entry name" value="Arf"/>
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        <property type="entry name" value="RASTRNSFRMNG"/>
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        <property type="entry name" value="ARF"/>
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    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="42"/>
            <end position="61"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="73"/>
            <end position="96"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="133"/>
            <end position="154"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="174"/>
            <end position="194"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="206"/>
            <end position="228"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="289"/>
            <end position="309"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="316"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="401"/>
            <end position="421"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="433"/>
            <end position="453"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="465"/>
            <end position="490"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="510"/>
            <end position="534"/>
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    <feature type="binding site" evidence="7">
        <location>
            <begin position="606"/>
            <end position="613"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
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        <location>
            <position position="613"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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    <feature type="binding site" evidence="8">
        <location>
            <position position="635"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
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        <location>
            <position position="661"/>
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            <name>GTP</name>
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        <location>
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        <ligand>
            <name>GTP</name>
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            <dbReference type="ARBA" id="ARBA00004141"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    <evidence type="ECO:0000256" key="7">
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            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
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            <dbReference type="EMBL" id="KAJ4373245.1"/>
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    </evidence>
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            <dbReference type="Proteomes" id="UP001140560"/>
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    </evidence>
    <sequence length="805" mass="88718" checksum="2D7699D27ED624DC" modified="2023-11-08" version="1">MNDASSVKSDTSSLSLDDADRRYLEALSDRDHVATKNTRQPLGLFSVVAFILQQVIGTGIFRTPWTVMQATDSVGITLLFWAFGALSAVAGTVVYIEFGLTTPRHLIDGRVEPVVRNGGDLNYVTYLIRRPRFFVLSFYAVNYVLLASTAANALSFGDDVIGGQGTNRGAGKDAAARGLAIIAVTIPCFLHAFTRRGGILLNNVLVVVKVAILCTFPIMAICVLSGVANTNHAKENLSIDKSFSQVHSNVDSYTQGVLAVFYAYSGYNQANYVLSEIDRPRKNFPRGSIIAVGTVCVLYMLVNLSYMIVVSKDQQLASSDISLAFLINIVGPYYAPKILVTFTTISSFGYVLGMTFTASRVKQEIAKEGVIPFAKFFGENRTLFRRHRTKNMVDQPEPTPIGALFLHWICAVLLILITWAMKPSSAYRILANLYVYLVDVVPSFIMAVGMLYLRCFTNWSAKSPLPSWLSVTAASVYTITNGFPLVAVWIPPFRSSDKAVHDIIPGLPWYMTGLLAWVLLGCCVLYWVSFRFLLPRFGARKGKEFVVEREPVFRTQNGERVQWHEIVLHSWVVKSEPEKRAGSLPTLRLPPSTNTITTEYSVLLLGLDNAGKTTLLEQIKGTYTPSHPNLNTVPTVGQNVATIALPPPNPPIYLKIWDVGGQHSLRGLWQSYYSSCHAIVFVIDSSDVGNATLADLSAHDAALNEDIGRLDECKLVLESVLANEDASGVPILILANKQDREDCVEVVRIKEGFVRRVFEGEKGGNVRDSRVLPCSALTGTGVQEAVEWLCSRMAFNKEARPPLMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPL3</accession>
    <name>A0A9W9CPL3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="12">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">N0V83_002055</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="12"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="270392at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02735">
        <property type="entry name" value="RNAP_I_Rpa1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01435">
        <property type="entry name" value="RNAP_I_RPA1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.100:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.180:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047107">
        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015699">
        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF11">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="14">
        <location>
            <begin position="366"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1369"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1705"/>
            <end position="1727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="141"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="154"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1379"/>
            <end position="1397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1414"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1457"/>
            <end position="1468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4374976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1727" mass="191219" checksum="1C4645F6498F12E5" modified="2023-11-08" version="1">MNTHQPVSSAIQGVEFGFLAAQDIRSLSVKRITNPTTFDTLLHPVPGGLHDAALGAFLDNPCSTCGLTTMGGCPGHCGHIELPVPVYHLTFLDQLLRLLRGKCVYCHQFRLARVQINEFVSRLRLIRCGLVQESNEMHEHIDINKGKTGKREADDSETDDDNEDIIGQRNNYVKRCMKRAGISKHDARSMREKNDTIAESRRVVLKEFYAAVAAGKKCKNCQGINPTFRKDRGIKVFRRNLSAKEKALMAQAEKRMQNPLDILARRDAKAKKQPVHADEGVADMSEEDEDIEMDDFQEADGSLVATESGTAQKSRTRVDAADSSQEYITAAEVRAAMILLFENESEMVRLLYSPYASKSLVNVSADMFFMEAVIVPPNKFRMEDKTGDSIAESPKNSLYKGILNACDAMRQISNEMKGQENEAGYRRRNFDDLQTQWVNLQGAVNALIDRDANPTQGRAAVTNADGIKQHLEKKEGLFRKNMMGKRVNFAARSVISPDPNIETNEIGVPPVFAMKLTYPEPVTNHNFYDLKEAVLNGPDKWPGAHAIENEFGQVIALRKKNYEERLALANQLLAPSSTYSNGSKNKKVHRHLNNGDVVIMNRQPTLHKPSMMAHRARVLPGEKTIRMHYANCNTYNADFDGDEMNMHFPQNELARAEAMTVADTDHQYLSATAGKPLRGLIQDHISMGVWLTNKDTFFTREEYHQLLYSCLRPEDGHTTSGRLETIPPVIWKPQPLWSGKQIITSVLKNIKPAEYQELTLTSKSATSGALWGANSEEQTVIIKDGQLLCGIMDKNQIGPAGGGLINGIYEAYGETIAGRALSIIGRLLTKLLHMRAFSCGVEDLILTEEGDKARLEELRKAEKLGFEVASKYVTLDSEKIDSTNPELRRRLESVLRDDSKQHGLDLLTNTANQKISGAVTKACLPGKLIKPFPKNQMQAMTGSGAKGSLVNANQISCNLGQQVLEGRRVPIMVSGKTLPCFKPFETSVRAGGYIVNRFLTGIRPQEYYFHMMAGREGLIDTAVKTSRSGYLQRCIVKGMEGLHVEYDTSVRDSDGTMVQFLYGEDGLDTTKQKYLNDFKFQAENFLSLAQALQTQEAFAQVYSPEASEHNKKAAKKARKGDVAAMDPVTAVYTPSRHSGSTSELFLQAKREYCEKNPDKLLKNKRKDPEGEILKKSFEQMLDLKYLRSLVEPGEAVGVVAGQSIGEPSTQMTLNTFHLAGHSAKNVTLGIPRLREIVMTASAKISTPSMQLYPHPELSKEDNEKFAKSITRLPLSAVLDKVTVTETTGPGAQFGQARSYKVRLDLYPAKEYTQEYAIKVRDVAESIEQKFCPRLQKVIRVDLKKKGENRSLSTANSAAIPTIGESLRTIDQQTSGEGDPAADNEGGLDDDESDDGVGDGDASNAKIRGRREDSVGYEEPEEEEQAINDNLDRAEASEDDETYGGSPKPSRATTPDSESSDDEDDEDEQILVPTDEASDIRRDRICTKCPDVYDFTFDDRKGAFCEITFEFPVGTPKLLMLHHVEAAAEFATIHVIPGITAAAYASKDVDEPVINTEGSNLIAMREYSHIIDVNRILTNDIASMLHLYGVEACRATIVKEMHAVFSGHGISVDQRHLNLIADTMTKGGGFTPFNRIGLKGNVSPFMKMSFETTVGFLKDAVLERDWDDLKNPSARIVVGRLGGIGTGAFDVLAPVTVVDPLNEGSEDDEVEVLEGVEGGEDEDVTMND</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ45</accession>
    <name>A0A9W9CQ45_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pzh1</name>
        <name evidence="10" type="ORF">N0V83_002950</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PPP phosphatase family. PP-Z subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ45"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000006">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF484">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE PP-Z1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="319"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="505"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="24"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="514"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="548"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4374209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="673" mass="74111" checksum="7A57879CF0C12A15" modified="2023-11-08" version="1">MGNQQSSGKKDKGDKGGTEGVPLGREHYRSFERSDTKDSTRSLRTSLKSKIPGTKSESPRNSVGNISDVNGKIDKSDAASTKSDKSRGTRGSRRNSTASAAPEAVDETINKVDTPSDEPQPPPSPVHGTTLGGGHEGVSKAQRTGEVDHVSDVPPTGVPPPLASAQPGESILQKKDQPIPRLPEPPAPTDGSGSPMEISALKTMDLDDMIQRLLDAAYAGKVTKTVSLKNAEIFAICSAAREVFLSQPALLELSPPVKIVGDIHGQYTDLIRMFEMCGFPPNSNYLFLGDYVDRGKQSLETILLLLCYKLKFPENFFLLRGNHECANVTRVYGFYDECKRRCNIKVWKAFVDTFNTLPIAAIVAQKIFCVHGGLSPSLSHMDDIRQIARPTDVPDYGLLNDLLWSDPADMENDWESNERGVSYCFGKKVIMEFLQRHDFDLVCRAHMVVEDGYEFFTDRVLVTVFSAPNYCGEFDNWGAVMSVSGELLCSFELLKPLDSSALKSHIKKSRNKRQSMLNSPGHESSISAESPILAKSTTSSREQDDSDLADKYKTTDEPEKMGLSQRLRRVRRRPGSGQLGAQEDGAATPPPKRSGHIRANTLSEPWGGPGNNSAASFAQRLQVYGWEREQQRQRELDEQRAMWETTLPRALTDHIGFFSSGHAFDDIHSDGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ98</accession>
    <name>A0A9W9CQ98_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Altered inheritance of mitochondria protein 9, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Found in mitochondrial proteome protein 29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_001874</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AIM9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ98"/>
    <dbReference type="OrthoDB" id="2831558at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051035">
        <property type="entry name" value="Mito_inheritance_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36091">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36091:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="7">
        <location>
            <begin position="121"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4374798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="366" mass="41771" checksum="0318D4C593479CB2" modified="2023-11-08" version="1">MDVRDQIRCVGAICQNLKELVDLNFPAYGSLYFANTPYVAARKLPFDQDFSIGPHCGAIYWDCNVGQARHYHSVDPNQGPWPDLAAYYNGLIDTGISRLPPADPSPQRPRYRGSIRTHRRLLEDGRAVLSKMAMDPRIQQAAVPVIFHPDLHKRNIFVSEEDPTVVSAIIDWQSSCIEPAFWYADEVPDFAQPIPDPLDEGRIEPKSEACAEAFDVSVRSLAPKLATARSMDENLFRPFRYCYRTWEDGAVAFREELIQTSLHWKELGLMEPCPFPLPCPDELARHQKDYKRFEAAHQLKHILSDLLNTASDGWVPSEQWEATELAHREVFAGILQEVIGNEQPDDDEPIKGEDDLREIWPFDLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQA7</accession>
    <name>A0A9W9CQA7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">5-formyltetrahydrofolate cyclo-ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQA7"/>
    <dbReference type="OrthoDB" id="433414at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13017">
        <property type="entry name" value="5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13017:SF0">
        <property type="entry name" value="METHENYLTETRAHYDROFOLATE SYNTHASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4374389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="33742" checksum="6189BF501F4B3DDA" modified="2023-11-08" version="1">MTNPVSSDERRKLIWARVYQELLHKAVPDSRFNHDFLSFTPDFRGSSSAVDRIVALPCYQNASTLLVTSDNSLEQLRCRALKDGKRLLVATYRLRRGFVLLSSARIREDKYELAACLDGMEKPDVGRTVSLAQIRDENICIDMCLLGGLAFNEQGVVIWEGHSLFEVQWALLQDIKSLASNVPVVAIAHTCQVVDEGRLGVERIIPDKAGEVQCDYVMTPDQSFHVEAPYRPTKGIDLEAIDQEALDNIPPLQELKGIRMMEQIMANGGFGQANEKETSKAPTAEEQMGIGMVEKLMKGYKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQS3</accession>
    <name>A0A9W9CQS3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_002423</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQS3"/>
    <dbReference type="OrthoDB" id="4064873at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000435">
        <property type="term" value="P:positive regulation of transcription from RNA polymerase II promoter by galactose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF9">
        <property type="entry name" value="TAH-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="5">
        <location>
            <begin position="110"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4375337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="231" mass="25673" checksum="2F35268626F110AF" modified="2023-11-08" version="1">MEKKVWHILGLMAMLILLTFLLVYIGDTASLSFLDFLRRHLRPYVGATSFTDGERQNPMLENDVAHVMSVETNLNVEEEEALFRSYSEATSGLLHLFSSADSDDFMELTGADTKREDLAAHDMVLAIGAQARANGPRDAQTAAMYFSRAQSIAFEDMLTHTSLSMVRLFILLAFFALGACQQSAAFMYLGVASKAAIVLGLHQPMSWKRLQQANGPNLRYSVFLSNSLNSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRE7</accession>
    <name>A0A9W9CRE7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zona occludens toxin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRE7"/>
    <dbReference type="OrthoDB" id="2316594at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957:SF1">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="491" mass="54033" checksum="E31DFE601EED7C50" modified="2023-11-08" version="1">MRLRQHPTPRRETVESTMASESDTSSSPDSSDSTGSASSELDFSLIDQLIAVKDRTTGTDSALKRMDRDIGHAPLLSDDIVTSQSKDLVPQCAFLGTFKEDSDASVTRIFQNTNEPFSTFICGVQGSGKSHTTAVMLENALVASDHLGRLREPVSALVFSYGDFSIGGLGFNISEAAFLAAEKEGFKDHEVKKLTVLRSASNRAIETYYRRFPKVEIIPFKINAQALDVGTLLTLMAVDDKVEVPLYMAKIESILRDIATKSKTGIFDYKLFKKKLEAANFDQHQKRMLDQRLSLLESFLDVAGQAPEPQFRQGEITIVDLSDPMVTASTACILFKLCLGRFMQSSAPGKMVVLDEAHKYMLDNPGSLVLTEYLKTLIRLQRHKGARVIVSTQEPTVSTDLIALCSTTIIHRFTSPAWYAALKKHINAMDDDKAIMEQIEGLETGEALIYSPTAVLGKNDDGTLEKATGRLLKLNIRMRVTSDGGESVMAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CSE1</accession>
    <name>A0A9W9CSE1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000837</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CSE1"/>
    <dbReference type="OrthoDB" id="5397183at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4378007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="494" mass="54695" checksum="EA65C526E478EB81" modified="2023-11-08" version="1">MDTGPSSSFTAVNHQWTQLPTPQSTASVSREQEQVRPRASAHSRRRHNPAVAQYLGLGNSHEPTHLEKYAPLPDVLECPPTSSTRKRRRLAERTLSNAGNTGRQDQRTVSQSFHLTKHAFNPALTPAKTSASALNPAQTKAKTSTHASQPTATKPTTSEVQTLRVASAYSPEHHTNLTVENPDTVLHHGHGTAPTRDIHIQSDNQDTVTVQPPEQFIDHQLFDLSNDEEDAYGSVQFSSDAIQPQVGGSDFDDSELDDALLMLTTGIDGPDNVHEESSSTVTSSTQSEDSDRHYILSSSADATTDVKEFSSPSRSQCVSRNFVSPVTPRTRLLAAACNASVAENWKPIVRSPFPEPVRDRSPIIGLSACTMLRTCFRIGEAVNQSCQATKSGKHITIELYARILNSDRTDTTQNFTFCDLFHAKPPYIKAVYEDIIWKPVQLFDYDSRRLLQEGRICRCIGTMKQEGKAWVMTVLNIWEATSEDIKWVEGIVNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXN9</accession>
    <name>A0A9W8XXN9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ankyrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_010351</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXN9"/>
    <dbReference type="OrthoDB" id="1577640at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054471">
        <property type="entry name" value="GPIID_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039">
        <property type="entry name" value="AMELOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10039:SF16">
        <property type="entry name" value="GPI INOSITOL-DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22939">
        <property type="entry name" value="WHD_GPIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="4">
        <location>
            <begin position="8"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase winged helix" evidence="3">
        <location>
            <begin position="182"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="372"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="406"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="439"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4362258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="486" mass="55234" checksum="490DCE0005EA7EC9" modified="2023-11-08" version="1">MAYKESLTSAKSGRPSWDVLKGAVNWFATNYKIKILIDALDECQGREDVLAFLDSIRNEKKAISILVTSREDLDVKDAIDGYRGLRMEDRRSEVDRDIESYIDHRLATNVNLNKFPEAIKVDIRCSLLNKSEGMFRWAQCQIDALSRLRTVKSIRAKLDRLPTTLNETYANILKSVQEEDAELLRRILLWVSFTIVPLTLDELQEAIAVEAGMRTLKEVKESRLNDPKDILSLGSSLLRVCETGHVKLAHLSVRDYLLSQDIKTHPDVAKFSLDHGEANRELATNCITYLSLDGLANGPTKTQDDWERRLDDQALLKHAAKGWTYYLRDADQTSELRKLVSDFFTVRSRPIFMSWIQILNSNWIFRWDEYPRHATSLYYAASFGLLDVVKELVKTGADIDEPGSRFGGTALHGATLREHTSVMELLFEAGADPSKADFNRVTPLHTAARIGNAGVIRLLLKFRASKTALDSLDFFMVERMMILEIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXP0</accession>
    <name>A0A9W8XXP0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ribosome assembly protein rrb1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rrb1</name>
        <name evidence="8" type="ORF">N0V83_010525</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXP0"/>
    <dbReference type="OrthoDB" id="2161379at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051972">
        <property type="entry name" value="Glutamate-rich_WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45903">
        <property type="entry name" value="GLUTAMATE-RICH WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45903:SF1">
        <property type="entry name" value="GLUTAMATE-RICH WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="7">
        <location>
            <begin position="86"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="298"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="343"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="398"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="151"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="51"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="160"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4362432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="492" mass="54007" checksum="F92E67D0BC50D58A" modified="2023-11-08" version="1">MAKRPADTEQEHEAVKNGDRPMELDQDEAGDFEDEFEDEFESEDEIFEAGVDGRPDEEREAEERENGMDIDQGTFIPGRHKLSPGETLNPDLSTYELLHTLDAPWPCLSCDIVPDNLGSDRKTYPATVYAVAGTQAARGRDKENHIMVMKMSSLSRMEKDDDDDSDDDSDDEASDPILETKSIPLTSCTNRIRAHQTPQSTSAKPPTTITAAMTESGQVLIHDVTPHLTAFDTPGTTISPTQNKPICTIRAHKANEGYALDWSPLVPEGKLLTGDVAGNIFATTRTQGGGFVTDTTPYTGHKGTVEELQWSPTEKHVFASASNDGTVKIWDARSKSRKAAVSVQVSKTDVNVLSWSHQTAHLLASGADDGEWAVWDLRQWKPSTSMASDAKPSPVASYTFHKEQITSVEWHPTDDSIVLVCAGDNTLTLWDLAVELDDEESRDTAGVQDVPPQLLFVHYMEQIKEAHWHPQIPGTIMATGGSGFGVFKTISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXP9</accession>
    <name>A0A9W8XXP9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_010366</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXP9"/>
    <dbReference type="OrthoDB" id="5427350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053143">
        <property type="entry name" value="Arylsulfate_ST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039535">
        <property type="entry name" value="ASST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35340:SF6">
        <property type="entry name" value="ASST-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35340">
        <property type="entry name" value="PQQ ENZYME REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14269">
        <property type="entry name" value="Arylsulfotran_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4362273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="507" mass="55963" checksum="0EDAEBF562EB3721" modified="2023-11-08" version="1">MFASLAYLLPVLAQNTTSLWPWQTFKSLPGLEPPVLSINKTGETSPGMIFFPQSGGEAHNYSVNIFREDGELVWVSGYGDYAAFRRTTLFGEPVLAFFKGVSFPEPWGFGYGIVYILNQQYENIYNVTLWNSTYPLQSISPLYDPTQYQPFSWIDMHENLITPEGTMFVSALNVTPWDLSPIGGPKEGWVVDSIVLELNVTNSEILWQWSHLNHVDEVPLASAIPTYPLGELGRNSSYPWGPFHVNSVERFEDGSLLISSRHYCSIFKVNRNGTVEWTLNGYSGGDFTLVNNLSFCYQHDARLHPSRSPNTTTVSLFNNDNSAVVSHVNQSTGIFLSIDERTMTATLLQEFADPDDAIYSVSQGNMEVTPSGNIVLGYGSVPKLKEFDQAGNVVLSVSWGEAGAVQSYRDYKAGWIGKPSAKPDVFACKADNGTEIYMSWNGATEHETWTVFGGAVNGSLSEVASVERTGFETRAYVAKTLEFARVEARGNTIKTGVSHIVGVSGVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY92</accession>
    <name>A0A9W8XY92_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Peroxisomal hydratase-dehydrogenase-epimerase</fullName>
            <ecNumber evidence="5">1.1.1.n12</ecNumber>
            <ecNumber evidence="6">4.2.1.119</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Multifunctional beta-oxidation protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">FOX2_3</name>
        <name evidence="23" type="ORF">N0V83_010073</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Second trifunctional enzyme acting on the beta-oxidation pathway for fatty acids, possessing hydratase-dehydrogenase-epimerase activities. Converts trans-2-enoyl-CoA via D-3-hydroxyacyl-CoA to 3-ketoacyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a (3R)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32711"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57319"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="1.1.1.n12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a (3R)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:26526"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57319"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="EC" id="4.2.1.119"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.n12" evidence="5"/>
    <dbReference type="EC" id="4.2.1.119" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY92"/>
    <dbReference type="OrthoDB" id="3592703at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018812">
        <property type="term" value="F:3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03448">
        <property type="entry name" value="HDE_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05353">
        <property type="entry name" value="hydroxyacyl-CoA-like_DH_SDR_c-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000410">
        <property type="entry name" value="Peroxisomal multifunctional beta-oxidation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000013">
        <property type="entry name" value="Peroxisomal multifunctional enzyme type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000185">
        <property type="entry name" value="peroxisomal multifunctional enzyme type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054357">
        <property type="entry name" value="MFE-2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051687">
        <property type="entry name" value="Peroxisomal_Beta-Oxidation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024">
        <property type="entry name" value="DEHYDROGENASES, SHORT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024:SF2">
        <property type="entry name" value="SCP2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22622">
        <property type="entry name" value="MFE-2_hydrat-2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0456">Lyase</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ketoreductase" evidence="22">
        <location>
            <begin position="315"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="761"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00055743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00073871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00081853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ4362956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1008" mass="109730" checksum="D451B3266DFE0620" modified="2023-11-08" version="1">MAAQELRYDGQTVVVTGAGGGLGRDYAVFFASRGANVVVNDLGGSFKGEGTGSAAADKVVEEIRSAGGKAVANYDSVENGESIIKTAIDAFGRVDVLINNAGILRDISFKNMKQSDWDLIYKVHVLGAYKCAKAAWPYFRKQKYGRLISTASAAGLFGSFGQTNYSAAKLALVGFTETLAKEGLKYNILCNVIAPIAASRMTETVMPPDVLAKLKPEWIVPLVAVLTHKSNTETGAIFEAGGGHIAKLRWERASGALLKTDDSLTPGAVAAKWKDVVDYSKPEHPQGPANALELLEQASQLPPNPKGEEVDFKGKVALVTGGGAGLGRIYCLQLAKRGAKVVVNDLVNPDNVVQEIQKLGGEAVGNKADVQDGEAVIKTAIEKYGRIDILINNAGILRDKAFANMTDDQWDMIHQVHLHGTYSCTKAAWPFMLKQKYGRIINTTSTSGIYGNFGQANYASAKCGILGFSKSLALEGKKNNIFVNTVAPNAGTQLTRTIMPEEVVQALKPDYNAPLVILLASDKAPEPTGGLYEMGSGWFAQTRWQRTGGHGFPIDVKLTPEAVLAQWERITNFDDGRADHPYDNASGLKSIMANMENTSKKSKKPKKDRKSNEEILKAQKEALAAKSEGTPFEYTERDAILYNLGIGAKRTDLPFVYEGDENFQVIPTFGVIPPFNAEAPFSFDDIVPNFDPRMLLHGEQFLEIRKFPIPTEAKLIAIPKLVEVVDKGAAGLVVYGSVTKDANTGEEIFYNESTVFIRGSGDFGGQKKGGDRGAATKTHKPPQRAPDVVVEEKTTEEQAAIYRLSGDLNPLHIDPQFSKAGGFPTPILHGLCSFGISGKHVLQKFGPFKNIKVRFAGVVLPGQTLVTEMWKTGNTVAFQTKVKETGSACAHSGATPAGLPIPKPELDFLKHGTFVIRELVYTRDEGTGIVEFEPCRKREQTGEHCKEMLEKPTYRKMTMKDGVGYMLIDPRKYDAQGQEVGRDERCPVCRKIDAKTYGRREYEQKVNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ97</accession>
    <name>A0A9W8XZ97_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ97"/>
    <dbReference type="OrthoDB" id="2555959at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="67" mass="7702" checksum="9D7A1B054DBE3ABC" modified="2023-11-08" version="1">MAVWQWFKSIPPRTRMIIGVGIMAYAGAGLYLSDRAEERFGLTPTEKDREELRKAMPKIATFEKRGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZT4</accession>
    <name>A0A9W8XZT4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009688</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZT4"/>
    <dbReference type="OrthoDB" id="3477286at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF64">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="2">
        <location>
            <begin position="90"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="482"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="889"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="325"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="482"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1121" mass="129360" checksum="0201CD52D0039038" modified="2023-11-08" version="1">MSSPANDSADLMERDRKTQGDNHFTHDAAPSTSHKKSRKPREYKTYNYDELPRGTGVIRLLKLFSSENSDAGVLCELFTPGEDEQNQYPYEALSWCWGVAEKTEYIRIQRNLKTYAKYVSSNLVAALKALRHPDRSRYLWVDMVCIDQENLNEKNHQVEMMDIIYGNAERVCIWLGAANDSSRTAFRFIKNEVSQLQSFDTLCNSTDSSKKWVALLDLMQRPWFSRRWVVQELALAQKAVIYCGHDRISWKKFAIAVELLVEVETATHRLSEVMKKDRQYQYIPSYFEFVSALGASLLVDANERLFRDYQEKRTSEPEPVIGSNPDPASESDSSPEASDSDESDTSTVPSPGAEGSTLTRSRSRIQPLLSLEHLVSSLTIFETSMPHDTIYALLAIAKDTTPRAVLPHATASSGRAHDGLEIHTQRKSYNVDYNLPYVDVCKEFIMFSIERSSHTDRSRALDVICRPWAVEERVLNKIREKKVQTKMKKERDEKRERDRAIRRASRSGRKVSIFTPNDKYVDIEDMPLPSWIPQLSGAPHAMAHRPGHAGLKLSRINADPLVGLPSLTQRNYSAAENKAVDLKALRFRKRATLGHFSMYVRGFMLDRIEVVEQVARNGAIPREWAELARWETAKGHPPDAFWRTLVANRGRDGKNPPVYYSRACEESFKKGGLESGSVDTTALIEYERNSVVAQFCRRVQAVTWNRALVKTEAGRLGLVRKDVEKDDLVCILYGCSVPVILRQSERKDDEVFTLELDQELLYLKNRMKKSFRRYFELKKRHEQQKGTEMKQLCGQWFKQTDWLRTNGFTPRKLGTDETKAELWRILENGLRDFNDWRFKNRQKVWPEQLRKMEQEKLDAQTVKGTPGKKQIDELLKKRKAREELRAQEYEAHKKRRKSRATIDHGLPPAFGSMVNGTVNGTTKDPPAIIKKKKPLMDWWAFGYALAAGRRWRKIVKERKVERAKFASRRTEVHWDIMRHDQYLAFEKGRRNEGNWVERPSTSISEIPSAIPEAERLAENANTQSNTSNTANGTHWSPEVLVSNVRPSWRARVNKDRLTETEKEEYNAKIRQNLRDRLGEEGYFSYQLLGECYIHGMMDGEAMLYQNEGDEEVIPSMVFEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y012</accession>
    <name>A0A9W8Y012_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">endo-polygalacturonase</fullName>
            <ecNumber evidence="3">3.2.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">N0V83_009649</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(1,4-alpha-D-galacturonosyl)n+m + H2O = (1,4-alpha-D-galacturonosyl)n + (1,4-alpha-D-galacturonosyl)m.</text>
            <dbReference type="EC" id="3.2.1.15"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.15" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y012"/>
    <dbReference type="OrthoDB" id="1546079at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000002">
        <property type="entry name" value="Endopolygalacturonase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050434">
        <property type="entry name" value="Glycosyl_hydrlase_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31884">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31884:SF1">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00502">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040954109" description="endo-polygalacturonase" evidence="14">
        <location>
            <begin position="20"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4364193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="385" mass="38923" checksum="F2F45F47883C7C5E" modified="2023-11-08" version="1">MVAFTLSLLLASLAVSTSATPTPEITAAPKAEVVKRATSCTFSGSTGAASASKSATSCATIVLNNVAVPSGTTLDLSGLADDTTVIFQGTTTWGYKEWAGPLLSIGGKGIKVSGASGHVLNGDGARWWDGEGSNGGKTKPKFFAAHSLTGGSTITNLNIKNTPVQAISIAGCDSLTITGMTIDDSDGDSGSLGHNTDGFDIGTSNNIVIDGAKVYNQDDCVAINSGTHKSITFQNGLCSGGHGLSIGSVGGRSDNTVDTVTFYNNQVTKSVNGIRIKASVGDTGTINKVTYNKITLSSISKYGILIEQNYDGGDLKGTAGTGIPITALTIENITGTGAVSSSGYDVVVTCGSSSSCTGWTWSNVSVTGGKTYGSCTNVPSVTKCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0P8</accession>
    <name>A0A9W8Y0P8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_009327</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0P8"/>
    <dbReference type="OrthoDB" id="9992527at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047122">
        <property type="entry name" value="Trans-enoyl_RdTase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348">
        <property type="entry name" value="HYPOTHETICAL OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348:SF2">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C2E1P3.01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="5">
        <location>
            <begin position="15"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="4">
        <location>
            <begin position="141"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4364730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="318" mass="34199" checksum="C5F4F7242F19CA17" modified="2023-11-08" version="1">MLLPHDTLARDTGLLIANNLPAILGNNIAGMITTVGEGVTSFKVDDHVFGMSSLPLHGSDPSDQSGLQEYAILNVNAIGRTPDNFSDEQVVTLPVNLVTAWTALFTESGFKIIPPFSPKVTEVDDHDISSILIIGGGTNAGQFAVQLARIAGIEKIVVIAGLKSKLELMGMGATSVIDRHAPLKEVAQQAQEIVGTDGATHVLDCANTELFTAIATLSATQRSQLRSLRHIKADELDRLKSQRPLCDAAFLGSVSMARLAPHTEVFWTNVPRWLEEGRLRPAKYLVVDGLEKVAEINEVLDGYRGLGRVGHQSVVRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0R0</accession>
    <name>A0A9W8Y0R0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_010487</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0R0"/>
    <dbReference type="OrthoDB" id="3690334at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="264"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4362394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="307" mass="35905" checksum="57C6C9D0A8C02826" modified="2023-11-08" version="1">MLSLGDFERALGKDFVKLIGPENYEEWFTDFFDIAYINGYRDFYLGTEPILEKPERPYFITERATHSEAPANWQYLLAVYKIQLRDWKDNNENARSACALLRAAVEPWIWNELSSDDTTSPQNAWKAIVATHCPPDDVRVARLLRKLDIIKLDDATALRPFLAQFEEVYNAIQRANGTFTRNMLFPMIHKALPRQYNTFIKLWNLTHSNVYIDDKLFKKYRSLLLEHADRNKAHWQAAEERGAPWGNDSANRSNDRITSDRPMCDYCGNFGHYMDDCRYLGRPDTPRCSFCNKLGHEEDICRKKQQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1X8</accession>
    <name>A0A9W8Y1X8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008693</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1X8"/>
    <dbReference type="OrthoDB" id="5135333at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33112:SF12">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="356"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="815" mass="92511" checksum="795DA5E9BEF2909B" modified="2023-11-08" version="1">MATVTYRPSPTQTKPQRVFALMTLTRPVGTDPFPPTEPWQGEIHGYRNTKAEMLNWMTELRIRHKSGSFWTFRNGEYTVASGGVPVQGFNAVVGRDVTDGGEKVWRLYWVEKREGLLHAIMNSLSEVSPEPRLCEVCADIEFRNIASAAQYCTKPPSEEEYKSALDKDLGTIEEVIERKERCDFCSLIATHYFKEETSLDGLFVGQCKLVCEIDFCISSSDHQNPVSRDRPPHTHTKTAWIQIHPDETVSSVPLPWELDSIDSRSQGVRQLFQLQAGYDRPDTEQSDTLSGIHGRRVRSLIDLNLPRKWLGTCEQEHIGRCSHGSSILAWETGFMPMFIIDVEDRCVTTTPPECRYVALSYVWGAARVLKHIGANTTFLRTPGSVSGPDVPMTIRDAICLVEGIGEKYLWVDSLCIIQDDFAQQQTQIAKMDQVYAKALFTIVAASGGDAGAGLPGVNLTHRSQAQEVLRLPNCHFYTLLYNGTEADNIVNKSTWAHRAWTMQELLFSSRCLIFTELQAYWKCPGAVWLEEVALEDTTSTNLEIFSGTANARFPDQALDPYDYSRLYGRLLGNYVQRQLTYKADLINAFSGITGRLSAIQNDKFFWGLPQSRFSWSLGWYLYAKQARNYATTRLATTTGAIQEVPFPSWSWAAWTSGDITPWISFLRSNQFSYKLQHQEGYEFLPVVDFWISEELGTLIPIEEPGRHADAVHTQHRFPWQGTECQLPEDIPSSRQRRPGLLHFWTSVAKVECDGTNGAEDAIVVCRTDDNRPMQDLVVLKVEWKEGIAYRVRSDLYPEADWLALETRHWRFVTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y223</accession>
    <name>A0A9W8Y223_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y223"/>
    <dbReference type="OrthoDB" id="10261348at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013169">
        <property type="entry name" value="mRNA_splic_Cwf18-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31551:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31551">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWF18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08315">
        <property type="entry name" value="cwf18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="242" mass="26487" checksum="A07A335E6AF95EEA" modified="2023-11-08" version="1">MSSHEKLTAAADARKARLAQLQSLKRKQAPSDDQDDDDTSPQFESATTSTSTALTRTEEEEPEHSITTKYLSGRNYDPTTRDVKLGFDTLPIADPTKTLEYKAQQLALETRAQQDADESASGSAQDGGGGADLYKLQPKKPNWDLKRDLEAKMKVLDAQTEKAIARLVRERVEAQKGAAVALQEQEQKKVGGGGKNNGVNGGEQGEGEEVGMEGAELVEAMHLKEQEEERDRERDAEDEVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2A5</accession>
    <name>A0A9W8Y2A5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Gig suppressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">GIS2</name>
        <name evidence="4" type="ORF">N0V83_007758</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2A5"/>
    <dbReference type="OrthoDB" id="3863715at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000012">
        <property type="entry name" value="Cellular nucleic acid-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051714">
        <property type="entry name" value="Znf_CCHC_NABP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23002">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="2"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="31"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4366123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="73" mass="7234" checksum="2021BE8DE64C117F" modified="2023-11-08" version="1">MKCYACGKLGHISRDCTAPNGGPLNTAGKTCYRCGETGHISRDCTQAEVNGDATAAPAAAPAQPAAAPTTAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2M3</accession>
    <name>A0A9W8Y2M3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lipin Ned1</fullName>
            <ecNumber evidence="5">3.1.3.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ned1</name>
        <name evidence="5" type="ORF">N0V83_007764</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the lipin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.4" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2M3"/>
    <dbReference type="OrthoDB" id="4567at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000063">
        <property type="entry name" value="Nuclear elongation and deformation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026058">
        <property type="entry name" value="LIPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007651">
        <property type="entry name" value="Lipin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013209">
        <property type="entry name" value="LNS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031315">
        <property type="entry name" value="LNS2/PITP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057124">
        <property type="entry name" value="Ned1-like_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181">
        <property type="entry name" value="LIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181:SF12">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04571">
        <property type="entry name" value="Lipin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08235">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24565">
        <property type="entry name" value="Ned1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00775">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LNS2/PITP" evidence="4">
        <location>
            <begin position="426"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="99"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="333"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="634"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="693"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="344"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="634"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="693"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4366129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="762" mass="83916" checksum="877C3B88061800B3" modified="2023-11-08" version="1">MNYVRSITGTVSKGWNSINPATLSGAIDTIVVEREDGSIACSPFHVRFGKYQILRPSDKKVEFRVNGQLQDYSMKLGEGGEAFFVFETSAHIPAEMQTSPLASPAASPEQKPIELPSDRAFDDPEPLDLDGTSVDRRRGRLSMSVPASENLHLEAAFGRPKSGDWSGFQMHRANTDDILPSAKASIAKSFDDSEDTKDHGPLQISKEDAIAWNRATRSASPPPVSASEALARAIKLSKKLFTSNIPNKVTDSGDLELDMTGYKSSEEDALRAEIIARKILSDELAGDYDIGALIGADENGNLWIYSSEEAKAAAMQKTSMNVLSEAALQSADAVSDPGYQSDSGRSDDTGDFPHVRRDSDSALGMSAPQSPEAHKSETRNYAKTLRLTSDQLRALDLKPGANTMSFTVNRSTCNAYMFYWKHDVPIVISDIDGTITKSDALGHVLNMIGRDWTHQGVAKLYTDIVNNGYNIFYLTSRSVGQADMTRAYLNGVVQENYRLPKGPVIMSPDRTIAALRREVYLRKPEVFKMACLRDIMQLFDKPVHQTPFYAGFGNRFTDALSYRSVNIPSTRIFTINSNAEVSLDVLSLNSYKTGYASMREIVDHFFPPVGLLVPAGGEAYTDFNYWRDRPLDIEDFTDSEDDDDDVDDTTEAASLRSEDEGSEVGEDLEASYLTQDSLDDLPNELQDSIIESVEADDYADADADAYVEGDEYGEEVDTDQDDEEAGITPTRELVDFDNDVGERVGLLSVKESTPTPRASPKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y301</accession>
    <name>A0A9W8Y301_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ysc84 actin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007960</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y301"/>
    <dbReference type="OrthoDB" id="443981at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11524">
        <property type="entry name" value="SYLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051702">
        <property type="entry name" value="SH3_domain_YSC84-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007461">
        <property type="entry name" value="Ysc84_actin-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629:SF8">
        <property type="entry name" value="DUF500 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G07310)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629">
        <property type="entry name" value="SH3YL1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04366">
        <property type="entry name" value="Ysc84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ysc84 actin-binding" evidence="2">
        <location>
            <begin position="137"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="321"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="405"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="503"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="531"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="582"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="631" mass="68935" checksum="3C78C694C6EC9858" modified="2023-11-08" version="1">MALWSKTKTQSKAGFDKVYNWVDKLGAPVNRLSNKVGSEAFWPTTLDIESDKAARILKSFCKDGFYQEEDRPTADAPKGKQRVLKKIPSKVIKNAKGLCIFTTMRTGLWVSGSGGAGVLVARKEDGTWSTPSGIMMHTVGVGFLVGVDIYDCVIVINSDKAMQAFQSIRCTLGGEISAVAGPAGVGGILDTEIHKRQSPLFTYIKSRGFYAGLQLDGTIVIERTDENERFYGERIGVKDILAGKVRHPPYEIKRLMETLRSAQGDTDVDESLIPDEAPPADFEITAPEDKAFGIPEAEDPDPFGVLALQEAGLDIREAGTLSRPTSDQFEFKPSPTSPIYTTFRNSTYRKSMDKSSRPGSQRSSWRASTISSILEPRTPSTMVDSSTQTADMGVQTDDLPPPPTVKKAPPALPPRQTQVRTPTPPPSDIPETRIQEATPSKEAIELKDNEVSPRVEKEKEADTTEPKSTETPADSMAFDDVDLDDDEHDDEHEEDVVIHEVHQAAAPQVVTRARVVQVAKPIPPKLPTRNPFRNRNSTNSEASLKTSHDASAGPSPTTEKHSPDSMPSLMRGDSTSSVSSVEGHRLEQIGDKLKPKYSDEAATEQEKKEEKEDAFHSLPASPVKPVPGGFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3C7</accession>
    <name>A0A9W8Y3C7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_007290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3C7"/>
    <dbReference type="OrthoDB" id="10524962at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4366765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="84" mass="9078" checksum="DBD95A5B8CA3C9EC" modified="2023-11-08" version="1">MLVPAYCPIDFGVHAIENSGVREEAKNVMAMESMPAMSVVDDDISIPVELGMDIPDIVLVGALALDIDMPDISMVVVKRFQSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3F2</accession>
    <name>A0A9W8Y3F2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_007471</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3F2"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044283">
        <property type="term" value="P:small molecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209:SF881">
        <property type="entry name" value="FI07970P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209">
        <property type="entry name" value="OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00682">
        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="216"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4366941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="369" mass="41995" checksum="2968DB4B76E458D7" modified="2023-11-08" version="1">MTLSTTNETLPAFTKLELRTAYGPVYRDVATTPPRECLPEEIPVIDVSRMFGDLKQRKSLATEIRAAAENTGFFYIKNHGIDEATIQTAHEQAKVFFHRPESEKQNVSRKKSKFFNGWLQRQTGRSSPSEALDNMEQFGWCYDPKYDPESKDLNAIPEDVKSGLQGEDFVWEGTEHLPNFKNDCIRYWQANLQLARRLLKIFALSLDLPEDYFDKVTTYPGSDSAFNFYPGVSSESIAKRSEDIGLGSHTDLQCFTLLWQDMNGGLQVLSKDGHWLKATTIPGTLVVNIGDYLMRLTNDRFKSTVHRVYNRSTADRYSMPFFFGFNFNETCGVLPSCVSAEEPAKYEPISCGESIRLRFKQIEESTTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3F9</accession>
    <name>A0A9W8Y3F9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009166</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3F9"/>
    <dbReference type="OrthoDB" id="16464at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05238">
        <property type="entry name" value="Gne_like_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050005">
        <property type="entry name" value="DenD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF043036">
        <property type="entry name" value="ErythonDh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43103:SF3">
        <property type="entry name" value="ADP-L-GLYCERO-D-MANNO-HEPTOSE-6-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43103">
        <property type="entry name" value="NUCLEOSIDE-DIPHOSPHATE-SUGAR EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="3"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="318" mass="34492" checksum="2539F45AFE5C9842" modified="2023-11-08" version="1">MKVLITGAGGFVGQILAKKLIETDSSTSLILADVKEPPNPTSSKNVQSVAADLTDISASENLIAQAPDAVYILHGIMSSGSEANLELGLKVNFESVRQLLDVIRTKRPGIKVVFTSSCAIFGRKAVENVATETDIVPMPESSYGTQKLMIEFLLNDYSRRGLIDGRIVRLPTVFVRAGAPTAAASSFVSGIVREPVHGKESELPCDPSIGIWLTSPRTLATNLVHAIMVPAEKFGHFRQVLLPGYTATSGEILDALEQVAGKEVRALVKEKRDETIQRIVLSWPAKYDTSRAKELGFSDDVGLEQTIRDFIEGEKQRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3X2</accession>
    <name>A0A9W8Y3X2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nucleolar protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">NOP2</name>
        <name evidence="13" type="ORF">N0V83_007463</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3X2"/>
    <dbReference type="OrthoDB" id="427002at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009383">
        <property type="term" value="F:rRNA (cytosine-C5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1170:FF:000001">
        <property type="entry name" value="Ribosomal RNA methyltransferase Nop2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011023">
        <property type="entry name" value="Nop2p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023273">
        <property type="entry name" value="RCMT_NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054728">
        <property type="entry name" value="RsmB-like_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="RsmB/NOL1/NOP2-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00446">
        <property type="entry name" value="nop2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF30">
        <property type="entry name" value="28S RRNA (CYTOSINE(4447)-C(5))-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22458">
        <property type="entry name" value="RsmF-B_ferredox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02012">
        <property type="entry name" value="RCMTNOP2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 10 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="12">
        <location>
            <begin position="296"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="592"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="53"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="596"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="622"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="639"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="674"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="720"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="513"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="388"/>
            <end position="394"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="456"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4366933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="734" mass="80627" checksum="9C2F2AD5D1991251" modified="2023-11-08" version="1">MGQGRRMKKQGMPEPLDESLVTRKRKDAPPAADEERKKRSRTAAREAKEAKTKAAPPKKVTPSKKAAAVNGAKTRVNGKPAKKFVPPPPSDDEDDISVSADGLDDDDVMEDGLQGVNVTGLDALGSASEDDEDEIDEDEFDSDIVDSDTEVRKAMWSDDEDDDEIEEKLTAANIAGLSRKLDMQKAIEEAEAQAELEEGNMQTNIANERPRILEDEEDEEGRPISNLVPQDIQLLRTRLNDTVRVLDDFKNLAEEGRSRTEYRAQMLKDICAYYGYSEFLADKLLNLFPAREAFAFFEANETPRPIVIRTNTLRTHRRELAQSLINRGVQLEPVGKWSKVGLQIFESQVPLGATPEYLAGHYILQAASSFLPVMALAPQENERVLDMAAAPGGKTTHIAALMKNTGCIFANDANKDRAKGLIGNIHRLGVRNSIVCHYSALEFPRVMGGFDRVLLDAPCSGTGVIAKDASVKTNKTEADFMKLPHLQKQLVLAAIDSVNHLSKTGGYIVYSTCSVTVEENEQVVQYALNKRPNVKLVETGLTFGKEGFTKIGGKIFHPSMKMTRRYYPHTYNVDGFFVAKFKKIGPTPANAVGVNESKSSGKAAANTTTQLEVIDNAPIREEGEEDESDFGGFDEEEDQKYIERAQAKDLRRKGKNPKAIPPPKSDKGANGTASKKDSKKDDAGAEEKASKKEKKAGGTEVAKPAKSNGVNGKESEVKSKKERRKSGEKKKGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y426</accession>
    <name>A0A9W8Y426_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Epoxide hydrolase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_006964</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y426"/>
    <dbReference type="OrthoDB" id="7130006at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097176">
        <property type="term" value="P:epoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010497">
        <property type="entry name" value="Epoxide_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016292">
        <property type="entry name" value="Epoxide_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661:SF35">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661">
        <property type="entry name" value="EPOXIDE HYDROLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06441">
        <property type="entry name" value="EHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001112">
        <property type="entry name" value="Epoxide_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epoxide hydrolase N-terminal" evidence="4">
        <location>
            <begin position="6"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4367382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="363" mass="40761" checksum="6C01A880B758C592" modified="2023-11-08" version="1">MASQPSPYTISVPDTALDKLKRKLSDADYPDELDTPDQWPYGSPLTDIKRLAKYWETSFDWRGAETKLNELPNYRKKVAVDGFGDIDVHFVWKRSSNPDAIPLLFCHGWPGSFIEVTKLLPLLEAGSAAGKPAFHVVAPSLPNFGFSQRITAPGFALSQYAETCHRLMLDLGYSKYATQGGDWGFYITRTMGILYPSHVIASHINMVRANPPSFTSHPALALQHAITPYTPAESAGLKRSDWFLNHGQAYRQLQATKPQTLSYAFADSPVALLAWIYEKLVDWTDGYAWTDDEVLTWISIYWFSTAGPNAHVRIYYEAGHNPTEKIPGRERASEWVDGVKLGLAHFPRELSVVPRIWGRTLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y463</accession>
    <name>A0A9W8Y463_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_007315</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y463"/>
    <dbReference type="OrthoDB" id="1658288at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047499">
        <property type="term" value="F:calcium-independent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185:SF1">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="4">
        <location>
            <begin position="19"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4366790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="259" mass="28923" checksum="9830F056A45A12A7" modified="2023-11-08" version="1">MKTVFEHNVNKHRVDWRGRLTARFDSDTLRGAIEEVVKSKGASRTDSFNDRNKRGCRTFVCTVAKEISDVTRLRSYSLPDEDDIPATICEAALATSAATTFFDPVSIGARMFVDGALGANNPVEEVEGEAANIWSSGTGEVKPLVKCFISIGTGNPGKKAIEDSLMKFLSKSLGGIATETEATERKFIARWAKHYDDNRYFRFNVDQGLQEVGLAEYREQGMIEAATDGYLRHQAQKSRMRDCVENLRQKQSVYIDDFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4I3</accession>
    <name>A0A9W8Y4I3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007307</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4I3"/>
    <dbReference type="OrthoDB" id="3794025at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="119"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="197"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="551"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="609"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="280"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="132"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="157"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="573"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="619"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="748" mass="81883" checksum="6AB0DBCFC2852A6A" modified="2023-11-08" version="1">MDTAPVGPPADIELLSDGQGLVESDLSETKSTKTDIETVEHVDPAPMVETSDCVQQIKLDSPFHSPKSDAGTNPSDTSLEEGEISENILEVTPASESEAVGTQNDIDDEIDFQEMTDIKEEPTVTDSSTEFKSVEADSRGMAKIEEASQVEEIPAPATSSTSASTETAAKSQESLLHAAVEQCQDLKVDQHIAAIASEEATDKDAKETDVETPADPVPTRSVPPHLRPHLQPPVPRQYGIADSKHATSTAEVSRIPDAPRAFRSQYAPYQPRYDNDSEPLARTRAQLMKTQNELNAARKSLAQIRATVESEQQQRIEAAFSSMLSTLLQKQGEAISSKASFEAKERDLSHRERMIQQFEIYLAEGQKQVKYQLEEQGIRAMDTVDRALIEREADLVAQKGISDFQGKLAIQAERLHLREAAQTMREQQYKALVRDAITSEVLQTAVSREKAAVLGREEYARGFDAGKEEGKKEIIDEVQQGAFIRGYAACRRVQTALHDLRAGRIARDSEKLDFLFDAGHVENLFNRGVQIGRLEGGNAAAMVNGGELKGLQKATKGAEEEEVKVDEQMVRNQPPPPQAPPPPSRPTFASQLRATAPTFHNGHFVLANNGASAPPTPTSAPTANTAPTSMTKMNGINGINTTGITNETNSINGTKSINLATNGTNGINSMNENAQVNSIKPPTPPFLARATQGIYAGRRILRYEDSDEDEDEHDNHNLHQNLNQNLELKQDQNLTQDHNRNQLLIDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4J7</accession>
    <name>A0A9W8Y4J7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_007000</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4J7"/>
    <dbReference type="OrthoDB" id="10621883at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4367418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="121" mass="14126" checksum="FD3949DBDF0AA638" modified="2023-11-08" version="1">MARHNDEVSKTLARYQLNTLYHAEALRLLGMIYWPKYSFPEFYGLDVLTAPKTIALDNGESRIVYVTRAIVFSHGFGGSWKVLRSGTEEWHTPWRSMEEFVKVLKREFGEMAGKFVEMFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4T7</accession>
    <name>A0A9W8Y4T7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GIY-YIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4T7"/>
    <dbReference type="OrthoDB" id="5412936at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="269" mass="30753" checksum="515099B0BEDC6B27" modified="2023-11-08" version="1">MGVWTFLSGRWSLDALKMIGGDLKFEGRSVYLDGVFGLDRRVRIYVGQATSLRQRVAQHLNFRYRRDNPSLHYHAMQYSIYNTIGCLATLPPSSMGNHALPGMDCPDLLLNLLEMWMCLVFRTLPSPISDIWLHEVEEASKGRKKGKEGEFGGLNIASPLDHGGKQREWVDLSDSDDPLFVEYMNLTQRPFTHQKKEEELKVKEEQDTPAERKKKYAEAARRQRNKDQDSIHVPQWVVLGSLAVAFGFIILNSRGGPQPQSQSRVHFRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4X9</accession>
    <name>A0A9W8Y4X9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_008351</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Mycotoxin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4X9"/>
    <dbReference type="OrthoDB" id="448450at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004165">
        <property type="term" value="F:delta(3)-delta(2)-enoyl-CoA isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051053">
        <property type="entry name" value="ECH/Chromodomain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43684">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43684:SF1">
        <property type="entry name" value="ENOYL-COA DELTA ISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0843">Virulence</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4365731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="219" mass="22914" checksum="8BC81BCE9501EB92" modified="2023-11-08" version="1">MISGKALSGHEASLITGKGKHYCAGRAMGGAEDTISAEVEAGGNLSAVLTAYPKVLVAAVHGASIGWGCTQLSAFDLVYAHQSAFFQTPFASLGLVPEGGSSYNFPKQIGKAQANALLLAGDRLSAYEAWVGGLITGVVDAETSDAFLRIVLEKVKRIGTFSGTALQMAKKLTADAADDVEARRKAGERERSDILAMFSQEDTKIRLAAFGGSKQKSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y545</accession>
    <name>A0A9W8Y545_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006919</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y545"/>
    <dbReference type="OrthoDB" id="410198at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052523">
        <property type="entry name" value="Trichothecene_AcTrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791">
        <property type="entry name" value="GNAT FAMILY ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791:SF14">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13508">
        <property type="entry name" value="Acetyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="93"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="235" mass="26310" checksum="E0C4D3377738503D" modified="2023-11-08" version="1">MPLELHKITPADTLAWTRIRAIAYYGPTHDLLHSGPISESSIQGVAEDRKHDLAKPNTWHWKIVDTDLDPSDDDPPDNCGRTIAIAVWSMHNVKAEGSNESTAIPAEKADNNDAPVFLPPELRLDALTSLLNPIRAAQKEVMGTEKQYFMLNQFATHPDHQSRGAGSLLLNWGLQKADEEGLVMYLDATETGRPVYEKRGFKLVRATEWDRVPWGGQGRDWHGQMVRQPQDVLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5J5</accession>
    <name>A0A9W8Y5J5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">uracil phosphoribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">FUR1</name>
        <name evidence="12" type="ORF">N0V83_007622</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPRTase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5J5"/>
    <dbReference type="OrthoDB" id="106623at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008655">
        <property type="term" value="P:pyrimidine-containing compound salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000023">
        <property type="entry name" value="Probable uracil phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001097">
        <property type="entry name" value="PRK00129.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="6 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="11">
        <location>
            <begin position="85"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4367092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="288" mass="31725" checksum="A834D04D2CE9CC17" modified="2023-11-08" version="1">MHNTKRNDEAGQVAVVRQGPAIRSPNGRHASPSQPLISVPALNMSSEAQTQPPSQCVGPEYRPTTQKPTATVSTPLKQANVHILPQTPQLIALLTMIRDRNTGRADFIFYSNRIIRLLVEEGLNHLPVEGHEITTPVGRTYSGVKFEGKICGVSIMRAGESMEQGLRDCCRSVRIGKILIQRDEETAKPKLFYDKLPEDIADRWVLLLDPMLATGGSALMAIDVLMSRGVPEDHILFLNLIASPEGAQNLADRFPKVRVVTAFVDAGLDEKNYIVPGLGDFGDRFYTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5Q8</accession>
    <name>A0A9W8Y5Q8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006793</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5Q8"/>
    <dbReference type="OrthoDB" id="10618037at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="445"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="489" mass="55155" checksum="65996E021A993EB1" modified="2023-11-08" version="1">MASPDYETLRMSLKRTAADFAVHAEYPEENHTPSELAKFSRIREVLHLGVMLCNMEMGTAGDIKTLIGDRVREIETETVPFIPNPFEINEAGKRPNTSFAKLLFGNNTESLADEEMLDVDPVDDCDWETGSNASSMHADDSGNAESQDSNNNKAVTIPNGDQPLKFESLQVELNDSLADFEEDFLDVANNYYEIQKVFRDGIKKSITPRKGQTPDTIWDEVEQKVNLASVRRQLRTPTRATESFQTSRQYGSNIIGAEDEEEDEEVRRMISEVAAEESDDDVYPLIPVIRHPRTPLKATPIPVRARNQEPTHDVPTMAMMFGTEDEQLRSNISTPTVFEHSPWGPPSQRRETLASTGRTSLAGQNPQASSSVQNHFSPPPRTNEPRTYTFARIQHAGAPLISAPPIMQRFYRSTAQNTLEELLNSTESEELKSLIQAYWEHVEKEIPASPSPEHQPEPQPLYSGSRPAWLRTTFGTTPTRWRDNQNGWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5U2</accession>
    <name>A0A9W8Y5U2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pre-rRNA-processing protein esf1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ESF1</name>
        <name evidence="8" type="ORF">N0V83_006373</name>
    </gene>
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        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5U2"/>
    <dbReference type="OrthoDB" id="431825at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039754">
        <property type="entry name" value="Esf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012580">
        <property type="entry name" value="NUC153"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056750">
        <property type="entry name" value="RRM_ESF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202:SF0">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08159">
        <property type="entry name" value="NUC153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25121">
        <property type="entry name" value="RRM_ESF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ESF1 RRM" evidence="7">
        <location>
            <begin position="171"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="NUC153" evidence="6">
        <location>
            <begin position="628"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="85"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="221"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="389"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
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            <begin position="663"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
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            <begin position="44"/>
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        </location>
    </feature>
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        </location>
    </feature>
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            <end position="159"/>
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            <end position="506"/>
        </location>
    </feature>
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        <location>
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            <end position="559"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009087"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF08159"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF25121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="718" mass="80808" checksum="34206F971045BAC7" modified="2023-11-08" version="1">MPLPKRQTIPAKAAKSSAPPDDSRFANFETDPKFRLPSKKHTKTKLDPRFSRLRSDPDFYNKATVDKYGRKISKEVGKKAIERLYEVEDEDEDEDEAEEQESKHPAEKKRDQAVLRELQRVQKEGFDPIRDGGLESSSDESSSDEEEDYEVEEQTELAGEDNAVPTGDISSRLAAVNMDWDNIRATDIMAVANSFVPANGRILNVVIYPSEFGMERLQREELEGPPREIFASTKNKDNITTLDDSPDASEDEGENEDRIKSNLQGDDTGEEFDSTALRSYQLDRLRYYYAVITCSSESVAKAIYDNLDGREYLTSANFFDLRFVPDGTTFDQETHDECKKLPDGYKPNEFTTSALTHSRVKLTWDEDDATRKEVQKRAFSRKEIDENELQAYLGTDESSSEDEEEAAKADKAASLRRALGLESSGKSTKPKQKASAKRDRDFPKPEGEMEITFTGGLLNDAGKGSVFENEIPLQETTMERYIRKEKERKARRKERAQARKEGRDPDAEVEDPAVDAAPEPADDDPFNDPFFADDPKEAAKASKLDAKTKRAKKREEKDQQAQATAAERANLELLMADEDGDAKMRHFDMNEIVKAEKAKKKGKGKKKGKKDEAPIVAVEDTFKINTADPRFAKLYESHEFAIDPTNPRFRETAAMKALLEEGRKKRKLGLGNEEDEVERGSGAKKVRKEREGGAGDAGGDDGDVKKLAARIKAKSKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5V2</accession>
    <name>A0A9W8Y5V2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006388</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5V2"/>
    <dbReference type="OrthoDB" id="3791184at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="333" mass="37578" checksum="FF53C83AB01BC426" modified="2023-11-08" version="1">MPSMVMDHAADIAQIVHKLKVERDTWEAVASKYKEAFEAQTKRLQELQDICFATQAELENERAQQRRLHVTSDPSENRRPGTVDGTGESSRAQSFGTAAILSTRKISSHRPQQSVGDCNNPLYKRVQEAMDQRNYGTALVEVERLLRGPLSSKARAEGLLLKSDILRASGPDEIYDALAACSEALELCDRLSELEAFLPRIQYQRGILYYELRMLHQARQAFSTVSDDELLSNTASDYDDIDYLRGGKRRSGFDENRTEELLVHLREHGFEVSSTSVGDFGKFSRLTYDHLEQASTYEHAAPTVCYCEGKTHVAAPSMEGFEERYALIQSVAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5X5</accession>
    <name>A0A9W8Y5X5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Serine/threonine kinase</fullName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">PKC1_2</name>
        <name evidence="4" type="ORF">N0V83_007245</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="4"/>
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        <property type="protein sequence ID" value="KAJ4367660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
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    <dbReference type="Pfam" id="PF02185">
        <property type="entry name" value="HR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00742">
        <property type="entry name" value="Hr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="REM-1" evidence="3">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="60"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="101"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4367660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="131" mass="14449" checksum="7A8E09EC8B8A3FAA" modified="2023-11-08" version="1">MASRKIDREKALINAANAMRQSTNNPAVLSRLDGQIKDGRRNIEYFESKLRDLDLQRTTSGMDNMSLQPGARKANNPLTPPPKDGWNGYVQEGQGGYGQPMMPPRAPYAPPPPGAPIKRQNFSKLGMLVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5X7</accession>
    <name>A0A9W8Y5X7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_006025</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5X7"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF70">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="334"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="381"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="448"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="61"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4368943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="508" mass="56915" checksum="6094D74520973B4D" modified="2023-11-08" version="1">MTDIEKPSAVVADDMEEKRGPALNERQQENAVGYHEYIEARDLEISDKENNRLRWKLDLTILPMFLITQALQFMDKTSLNYANLFGYQVALKLKGQDFQYLSAMVYAGYFFGQYPCGWLIGRYPAQKVIAISIFLWGVMVLIMTQCRSFSSALAVRFFMGIFEAAVTPGLTLMTGFWYTRKEIPLRQCIWYSSLGWGGIIGSYISMGISKLPASTKPERWELIFFILGGATCLWAIIVWLFLPDSPSNARFLSHRERLVAVRRVAGNDTGIKNKSFDKNQIKLGFLDPKTILLFISVFAAAIPNGVVNSFSTIIIRDMGFSTTRTTQLKSVGDAVQIVALLISGSIILNVRNSRLITATVANMLCTIAAALMAYLPRSNTWGRLVCFWLVNAQSVGFTVSLTTISSNMAGYTHRSLASALIFTAYCWGNFAGPFVVKANQAPRYEGATIGLLVGYAIKFACHLGLLLYMYFSNRRRDSTYGPPNKEASTEAGMRDLTEFENKDFRYVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y640</accession>
    <name>A0A9W8Y640_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y640"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="124" mass="13757" checksum="C4F1BB170CD23A88" modified="2023-11-08" version="1">MAKSYKTFTKAKLLSILSQKCANALAMLPPPDTFEFLLKRRDGEDTFALMVGNYATKENHTEKNKLYLEGYDTSSQATGGEASSPRPGEVRLVNGTRTTSPSFIEGPFTYTSHYGKATITVYRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6C8</accession>
    <name>A0A9W8Y6C8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6C8"/>
    <dbReference type="OrthoDB" id="3942467at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="221"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="253" mass="27728" checksum="A9206B7E8A98F361" modified="2023-11-08" version="1">MHLPYEPQIEEEEKDDSEMPEITINAATQIRGNGNIISIAQMDSARIANLIASMLNGGKGVEETSPSQQPQEHSPPATPTAEETTKKQVGSVHITVNCGATIIGDRNIVGPGLGDIARQMQLAQRNQALHAQQLAQQQAREQQQQQAQQEPPRHHHSQQQQQQSPQQQPQLRHSPPMLKMPLPAQRRETLYQAQQAIAQHHGTMYAAQGLLSLQAPTPPLSRSGSFGSEGSGSAKRKAEHEHDAEEVACKKQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6P3</accession>
    <name>A0A9W8Y6P3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006724</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6P3"/>
    <dbReference type="OrthoDB" id="10250990at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052174">
        <property type="entry name" value="Flavoredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43567:SF1">
        <property type="entry name" value="FLAVOREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43567">
        <property type="entry name" value="FLAVOREDOXIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="158" mass="17718" checksum="0B38035598997DE6" modified="2023-11-08" version="1">MQDAVNAIARTTGSDPVPESKLARGYTHCKDKFGRAKLTKLPSTKIKTPGIAECPVVMEAELVNEHALFTDQPIEGAILIFELRIARVSIHQELKLDGYRNRVDPDKWKPMIMMFCELYGLRNGKLAESKLAEIDEEIYRPYTGTTVAVDPLTGLEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6S1</accession>
    <name>A0A9W8Y6S1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial</fullName>
            <ecNumber evidence="20">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">SDH1_2</name>
        <name evidence="23" type="ORF">N0V83_006315</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15 20">Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 20">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="18">Flavinylated by SdhE, about 5% flavinylation occurs in the absence of SdhE.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 20">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 20">Mitochondrion inner membrane</location>
            <topology evidence="1 20">Peripheral membrane protein</topology>
            <orientation evidence="1 20">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="20"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6S1"/>
    <dbReference type="OrthoDB" id="71672at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000001">
        <property type="entry name" value="Mitochondrial succinate dehydrogenase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.80.40:FF:000002">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000482">
        <property type="entry name" value="Succinate dehydrogenase complex, subunit A, flavoprotein (Fp)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.100:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase flavoprotein subunit (SdhA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.100">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.80.40">
        <property type="entry name" value="succinate dehydrogenase protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011281">
        <property type="entry name" value="Succ_DH_flav_su_fwd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01816">
        <property type="entry name" value="sdhA_forward"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE 2 FLAVOPROTEIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632:SF51">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000171">
        <property type="entry name" value="SDHA_APRA_LASPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 20" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 18 20" id="KW-0274">FAD</keyword>
    <keyword evidence="6 18 20" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 20 23" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 20" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 20" id="KW-0813">Transport</keyword>
    <keyword evidence="5 20" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="21">
        <location>
            <begin position="50"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="22">
        <location>
            <begin position="499"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="55"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="443"/>
            <end position="444"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Tele-8alpha-FAD histidine" evidence="19">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000171-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ4369230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="635" mass="68912" checksum="3635EDFFCF16F30E" modified="2023-11-08" version="1">MMRAFARAVQRRQFGTRSSFLRQTNSRHRGFASVNSSEVKGLTVIDHHYDAIVVGAGGAGLRAAVGLTEAGLKTACVSKLFPTRSHTVAAQGGINAALGNMTEDDWRWHMYDTIKGSDWLGDQDAIHYMTREAVPAVVELENYGMPFSRTSEGTIYQRALGGQSLKYGKGGQAYRTACAADRTGHAMLHTLYGQSLKEGVQFFIEWFALDLMMSEGKCVGITALNMEDGTCHRMFARNTILATGGYGRAYFSATSAHTSTGDGSAMVSRAGLPLQDMEFVQFHPSGIYGAGVLITEGARGEGGYLLNAQGERFMERYAPTAKDLASRDVVSRSMNLEILEGRGCGPNKDHIHLQLSHLPKEIIMERLPGIAETAGIFAGIDITKEPIPVLPTVHYCMGGIPTNYHGQVLDVRDGKDVVVDGLYAAGEAACVSVHGANRLGANSLLDIVVFGRASALHISSNHAPSAPHHPSPPEIGLPSITELTTFLTASGSTPTASLRDKMQSTMQSTTAVFRTHDSLLSGNTSLASLESTFASDLKVTDKSLIWNSDLVETLELRNLLTNARQTSTAALTRTESRGAHARDDYQERDDERWMRHSLTWQAGVGDEVRYGMRDVTMRTLDEGEAKSVPPMKRAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y792</accession>
    <name>A0A9W8Y792_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ER membrane protein complex subunit 7 beta-sandwich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_005677</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y792"/>
    <dbReference type="OrthoDB" id="27095at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019008">
        <property type="entry name" value="Beta_sandwich_EMC7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039163">
        <property type="entry name" value="EMC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13605">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13605:SF4">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09430">
        <property type="entry name" value="EMC7_beta-sandw"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040805055" description="ER membrane protein complex subunit 7 beta-sandwich domain-containing protein" evidence="8">
        <location>
            <begin position="18"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="ER membrane protein complex subunit 7 beta-sandwich" evidence="9">
        <location>
            <begin position="31"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="216"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4369913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="241" mass="26081" checksum="A3DE187F964C5C09" modified="2023-11-08" version="1">MKASITALASLASLTSAARLLLQIPNTALVNPSTLPSTTHATLQSSGPPLDAYLTRSNTFNFNNVSSGSYLATVHCRDYAFEPLRIDVGIEEAVEGSGDKKEVIRVWQTFPGNEWDNKGESRGEGGNGLVVEVRPVTMKYFYQERQGFSPLSFLKSPMILMAIVSMALIFGMPKLMENMDPEMKQEFEDMQKNGVLGSGSTNTAQQIQNFDLASWMAGKTDSGSGSASARAQSPAGQAKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8H2</accession>
    <name>A0A9W8Y8H2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_005061</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8H2"/>
    <dbReference type="OrthoDB" id="5296287at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF68">
        <property type="entry name" value="MULTIDRUG TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01120)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="328"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="411"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="469"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="510"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="103"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4370540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="542" mass="58980" checksum="929A229944F71499" modified="2023-11-08" version="1">MATPASIPHAPQDAQHLQNEKVGSSYVQSHEADGTAEEAFTKDVEKGVESHAHSTHTDERTLSGDVPVEAEVQEQIDPNIVDWDGPDDPHNPQNWPAKKKWRIIAALGAVTLITPLASSFFAPGVPQVLKAFHETSNLMAAFVVSVYILGFAVGPLIIAPMSELYGRMPLYNISNMLFVIFNIACALSKSMGMLVAFRFFAGCAGAAPLTLGGGTIADMFPPEQRAGAMAIWSMGPLLGPVIGPVCGGFLVQAMSWRWVFWIMAIFGGAFGVVLFFVGRETFPITLLEKKAARLRKETGNQNLRSKLAKDIPPREIFIRAIVRPMKMLFLSPIVSLMSLYIAINYGILYLFFTTVTFVFEGKYHFSSGAVGLSYIGVGVGMISGMAVLGVMSDKFIKKAQAQGNAKPEHRLPLALTMPGAIALPIGLFVYGWTTYYGVHWIVPIIATSFLGLGNLCAMMTIQTYLVDAFTIHAASAIAANTVLRSVFGAVIPLSGLSMYDKLGLGWGNTLLGFISFAFIPVPIFFRFYGEKIRTNPRFQVQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8P1</accession>
    <name>A0A9W8Y8P1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DJ-1/PfpI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005970</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8P1"/>
    <dbReference type="OrthoDB" id="543156at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03139">
        <property type="entry name" value="GATase1_PfpI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052158">
        <property type="entry name" value="INH-QAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43130">
        <property type="entry name" value="ARAC-FAMILY TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43130:SF15">
        <property type="entry name" value="THIJ_PFPI FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14240)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040822417" description="DJ-1/PfpI domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="DJ-1/PfpI" evidence="2">
        <location>
            <begin position="141"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="298" mass="32558" checksum="4AFE84248941A78C" modified="2023-11-08" version="1">MRISNLSRLFLAIPATLAAPQQPDFSNTTTLPTHFGLLVFPHFQALDVFGPMDVLNTLSMLYSNTTMYLSIISKTLDPVSTAMMGMHGSVDFGESIMPTITFHKYLARSRKDGEDDNQEDNNKCEDGAGGYVVAKNKCPSHGHGSMANTTTGKNLGDIEVLIVPGGGGTRQNMTEEIAFVKELYPKLKYIISVCTGATILARAGILDGHKATTNKRSWAWATSTGLNVTWVPTARWVEDGNMFTSSGISAGIDVMYAWVSRVYGDEVAQYLSLSAEYDRETDSHYDLYGKIWDVPGAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8W1</accession>
    <name>A0A9W8Y8W1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_005867</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8W1"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11063">
        <property type="entry name" value="CYP52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047146">
        <property type="entry name" value="Cyt_P450_E_CYP52_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287:SF17">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="341"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="499"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4368785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="547" mass="61384" checksum="E0F1F704C1823638" modified="2023-11-08" version="1">MAIFSFTNIAVSLLSLYFARRIYWESTTGTRRRALAKQHGCLPAKQRLNRVLPNFLPDMLGIDMIISQYRAYKAHRMLEQEANDLRSAGAHTIQTSALGLNVFITDDPENVKALLATNFEVWSLGQSRIKRMSSYLGHGIFTNEGKAWKCSREMLRPCFERSAVADVSIFKKHTERLIGLLPLDGSEVDLQPLFHELTLDVATEFLFGRSTNSLGEGGGEDEGVKAENIKKWGFLGALLPDKKKKRSIKIIQDFIDKVIDEELCLHEEEKKNKKDTQRYIFLHELVTATQDRTVIRNELLNILLAGRDTTASLLSNLVWELSRQPTVLARLREEVDSVVGTSTTNENNNSNSDEDAGIHDIKNDHEHEHEHESDMDPPTPTYAQLKELKYLRAIINESQRLYPVVPANGREALHDTILPHGGGGGGGSGGDGGGNTPLFIPKASYVQYNTYSMHRRASLFGPDPETFRPERWLNNDSNDHQPLRPGWAYIPFSGGPRSCLGQNFALTEAMFVVVRLVQKCEIERRDEGVWREKVGLTCMGLGVVGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9Z4</accession>
    <name>A0A9W8Y9Z4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_004561</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9Z4"/>
    <dbReference type="OrthoDB" id="3943809at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4371344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="207" mass="23387" checksum="0ED2DED1C9213BB6" modified="2023-11-08" version="1">MSSFERKISLVGRAWEDKQVQEVYRECAPTEWWDRYTNSKRVVPTTFNPFFPSVEAIDPFTCRLAPGGTLQTWVHAVPNLGMVPNSLNLAKGIHLLSSLAEIGSNCALSIGASSDEKQRLDEQLMDRLAEQRVIAMKYGVAKKQHINASFTPAEYQAARQEFRSGRLGSGDSADRDTILRQFQKIFYSKYECAWNDEQKNNLSSICA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA27</accession>
    <name>A0A9W8YA27_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA27"/>
    <dbReference type="OrthoDB" id="5373857at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="112" mass="12546" checksum="6F3EE4A36CCF29FF" modified="2023-11-08" version="1">MSPLSVPKIFMAGGAVGFGYYLYMRQHWFPNPYKTPAVQSIEDRYSSGGGAPTHLPPMASKRGSATENDGRQHGPSKGPDSEWYKENMTNQPEEPVYPDKFYKQQYGNPKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAE8</accession>
    <name>A0A9W8YAE8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF3638 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004841</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAE8"/>
    <dbReference type="OrthoDB" id="3182339at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022099">
        <property type="entry name" value="DUF3638"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12340">
        <property type="entry name" value="DUF3638"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3638" evidence="1">
        <location>
            <begin position="5"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="181" mass="20632" checksum="370DC479289EC1AC" modified="2023-11-08" version="1">MYQPGLAQSMLATQAYFDNVTRDIIDEVDDNLSVKFELIYTMGSQESVDFAPERWLIIQQVLELLPQFAIQIQKHLPEAIDIQTFGEGKFPRVRLLRKNAADQLLKSLAEYIVDRGLPGLPTRSQPDAMRVAILRYITLPELDIEDINAVEKSNFWSNLTKFPLLLVRGLIAGGVLRFTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAN8</accession>
    <name>A0A9W8YAN8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004979</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAN8"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="219" mass="26465" checksum="E0CE3DC5F8F4DDC8" modified="2023-11-08" version="1">MFEAYCSLMLLPGEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEKEEEKEEEEETPPFRLKSSRQHRSSENELKQKLIVILEMATGDLRKLEEKEEGEEKTLTFRATFARQHLQIKDELVKMAEQSLRVHEESLRVREEDVRRREEDVERREENVRRRERTAPGGVVTVGEEEGSDGGEGEWVDVERQFLELFSREFLLRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB42</accession>
    <name>A0A9W8YB42_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF3752 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004305</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB42"/>
    <dbReference type="OrthoDB" id="73491at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022226">
        <property type="entry name" value="DUF3752"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046331">
        <property type="entry name" value="GPAM1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46370">
        <property type="entry name" value="GPALPP MOTIFS-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46370:SF1">
        <property type="entry name" value="GPALPP MOTIFS-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12572">
        <property type="entry name" value="DUF3752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3752" evidence="2">
        <location>
            <begin position="164"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="316" mass="34025" checksum="CF416E1844CAF2D2" modified="2023-11-08" version="1">MPSIGPTQPPQPAKRSRDDDDDAKRSSSPDSSDKRRRVIGPAAPPRSNSPARTSGPSLPPDARSRSSSSASDVGLAPPQPSKPARILGPAPSPAPLDERPPNPPEDDTSDSSDDDFGPAPPPAGVIYGDDDDGAHRPAKSAFDTDAQFTEAPKKAQRDDWMTMPPTQDDLAARMDPTKMRARKFNTGKGAGATGGMSSAWTETPEQKLKRLQNEALGIAPPSNASPSTATESRKTKEEERRARKMREKIDAARGKSLVEQHQDKGAGKEKEDDPSKRAFDYEKDMAVSGTVNHKQRREMLSKSKGFSDRFSGGSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB75</accession>
    <name>A0A9W8YB75_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_004093</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the sal C2H2-type zinc-finger protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB75"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000793">
        <property type="entry name" value="C2H2 finger domain protein FlbC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001102">
        <property type="entry name" value="Transcription factor IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051565">
        <property type="entry name" value="Sal_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23233:SF84">
        <property type="entry name" value="FI23031P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23233">
        <property type="entry name" value="SAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="310"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="338"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="206"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="263"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="206"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="263"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="292"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4372319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="376" mass="40523" checksum="4D0141BA0E0743C0" modified="2023-11-08" version="1">MAMAVDNRQQSQLNGLGYDHMRYPAPPHFTNPWVSAGTSQQQLYSTSVAPNVSAGLEHASQRPTSIALPYHGLAVTAPPMGQGITLPDGNFSTGNLLDTSQDLMSRGYTGGYATSAASNTQTYAPTSAPYSQVEYGNTERSPYTYQQDASRRSSHPSVPSIAFYEPVEAQRQRQSSLVDFSRMNTQPSRSSFSDALDASRGMVSLSQSDITPRNIYGSQGTSRSSTDSYGFPSTHSAHSSISSASYGANGYYGGEGSVTDYSSASESVDLTNSRTLPRPSALLGATMPPAPQSMMSQFSSKVSSSSQKKHKCKICDKRFTRPSSLQTHMYSHTGEKPFACEVEGCGRHFSVVSNLRRHRKVHKNDGAMDAPSPDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBB0</accession>
    <name>A0A9W8YBB0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Enoyl-CoA hydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_004324</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Mycotoxin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBB0"/>
    <dbReference type="OrthoDB" id="14970at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051750">
        <property type="term" value="F:delta(3,5)-delta(2,4)-dienoyl-CoA isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000004">
        <property type="entry name" value="Delta3,5-delta2,4-dienoyl-CoA isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045002">
        <property type="entry name" value="Ech1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43149:SF1">
        <property type="entry name" value="DELTA(3,5)-DELTA(2,4)-DIENOYL-COA ISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43149">
        <property type="entry name" value="ENOYL-COA HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0843">Virulence</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4371108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="25935" checksum="8901A9DA485BBF13" modified="2023-11-08" version="1">MWLNLSSIFRTLSHDPSVRAVVLTGSGDRAFTAGLDVQAASEGGTLTGAGPSDTARKANGIRRHILEFQSCITDLEKCEKPVIAVLHGVSFGLALDMSLACDIRVATSDARISVKEVDIGIAADIGTLSRLPHSVGNLSWVKDVALSARIFGAEEALRVGLVSSVYKDKKEAVDGALKLAALIASKSPVATLGTKEIINYSRDKPIVDGLNYTAVWNAAMLQTQDVKDAMLSGLQKRTPKFSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBC1</accession>
    <name>A0A9W8YBC1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pentatricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBC1"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="627"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="674" mass="75532" checksum="1B61185801BDD64F" modified="2023-11-08" version="1">MLAIWSRARQKPGTCRYISSVSNATAIARRRGQSGLRGAWAFGTPTSTFCYTAIFAAGLTIDANAKAARNRQWEEAFKDLRDALDRPLPKDNAIVEDVVGSRSDQVNLPHGYHGVPLEELPDALDWSIIQKVVGMELVDDPVLRLQETQSQLHDLAQGHWDDLRFDSRLPGAQSLEWPANTGRDLVQYHLPPQSLWAPDTLRLTAMRRRHSWKKLALQELSTGALIYTLISRINLSRYFQTASSDLERISSQLLEIASLTEEQASKARQEILDSIEQVHETDVKSAVDDVARASLQRGQPGIPSYFQDADGDFYDICQQMNNGIKKLLDQVPRGNDREEALAIAKICHNLLVSTASPDVQTFNILLAGLKRLRRPKLVDDVIAAFYVHKIRPNEITCREILGHYISELRADDFSRFVARMRGVGDALMLANPNITLNEASHDRLVRTSEGKVYQKVHPTPMVFGSLIGGVMRFAGFDRALDIYFEMKADGWGLDMPGLTRLLGDCIRRADWEGGTYTWEEINAIKAKAKPKDMAKAYYHMLGLCSVTGNTVAFNQVLNEVAKRGFDRKSILNAAKRTTDWVRKNKKDLAPAWAADNVLIAVSSYVHDAKSSTSNPKVEEVHNDDMFAQHTPSSTGIGEDEATVQIERQPPAHPNELWASWVEHEFGERPKDPET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBN1</accession>
    <name>A0A9W8YBN1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carboxylesterase type B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBN1"/>
    <dbReference type="OrthoDB" id="6846267at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142:SF4">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="1">
        <location>
            <begin position="23"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="424" mass="48043" checksum="D696094A2F33156B" modified="2023-11-08" version="1">MEVGTANLKPEALVPLISDSAFQAIMVLPSYRLNALGFLTGSELAAEASAYGEATGNMGFWDQRAALQWTHENISCFSGNPGNITVAGYSAGAYSAFQQLAHELWRLPTEKAIIRRVAMFSNSPGVKPKALEDQQEQFDEFLTRLGIAKDLEDKVKLTKLRALPYERLIEVQSEMEISEFRVFADDLFYPKDLIDRINDGQFARRMKSRGITLINGECKDEHTMYRRWRTPEDSYGAVYERLYAEFAPTVVRALMHHYCGPAKKLPTGYTSWQEFFGRLYAGIQVHNVQRGFHNALFEGGLQPGKDVLRYRFERRLGCVEERIPSEWGVTHLTDIPIWLWGSDFAGGLTNQEKEWLKGWNECFAAFINGTQVSWGSTKPKEVRRWGSDGETDIWEDSMWEEGIAFWKIVNSGSAKANSTGLSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBN3</accession>
    <name>A0A9W8YBN3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Class II myosin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MYO1_4</name>
        <name evidence="11" type="ORF">N0V83_004281</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBN3"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000015">
        <property type="entry name" value="Myosin I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000004">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000007">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF837">
        <property type="entry name" value="MYOSIN-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 8" id="KW-0518">Myosin</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Myosin motor" evidence="9">
        <location>
            <begin position="1"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="10">
        <location>
            <begin position="495"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="8">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4372507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="645" mass="73329" checksum="B8901AE59FBAF6EF" modified="2023-11-08" version="1">MCAVIGLQQAEQDNIFRMLAAILWLGNVSFSEDDQGNAAIVDQSVVDFVAYLLEVDSAHVNKALTIRIVETSRGGRRGSVYDVPLNCAQATAVRDALSKAIYFNLFDWIVERVNVSLKARGATAHSIGILDIYGFEIFERNSFEQLCINYVNEKLQQIFIQLTLKAEQEEYEREQIKWTPIKYFDNKVVCELIEEKRPPGVFAALNDACATAHADPTAADQTFVQRLNALSSNPNFQPRQGQFVIKHYAGDVSYAIDGMTDKNKDQLLKDLLNLLGQSGNTFVHTLFPNEVDQDNRRRPPTAGDKIKASANDLVTTLMQARPSYIRTIKPNENKSPKEYNEPNVLHQIKYLGLQENVRIRRAGFASRQTFDKFVERFFLLSPKCSYAGEYTWSGDYESGAKQILKDTNIPAEEFQMGVTKVFIKTPETLFALETMRDRYWHNMAIRIQRAWRNYLRYRIECATRIQRFWRKLNGGKEFIQLRDQGHKVLQGRKERRRYSLVGYRRFMGDYLGIGNKGGPGEVIAQAIGLSANEEVPFSCRAEVLVSKLGRSSKHEPRSLVLTKRNVYLVKQVLVNRQVQIQADRTIPVGAIKFVSCSTLKDDWFSIGAGSPQEPDPLVSCVFKTEFFTHLTNVLRGSLNLKIGDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCE8</accession>
    <name>A0A9W8YCE8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCE8"/>
    <dbReference type="OrthoDB" id="5360255at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004354">
        <property type="entry name" value="Meiotic_Rec114"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03525">
        <property type="entry name" value="Meiotic_rec114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="527" mass="57742" checksum="AF3B346701A61B77" modified="2023-11-08" version="1">MLSLPLAKLSVAEDADPAKQKFTWKHEQHDLTFILDSYGARGSSQLLKVVQGTQVRQVIELELMIKEGEEMARSMRDRGVELKADQLPVSAIVRCPLLAIRWQLPNKKVRRAQLKFRSSEDFDAAYSQLYQLGLRMSSSIESQAQTQAPITSSERAISSSKANPVATATPYHSASGSLTCPPSKLLEFSNRPYTATNAPTALESQLQEVAHRRPMSAYTSRSNETDNGVSLSTLLTPPVYFARPTSATSDVLGRTMNASVFPSSEWPNPTMEEIPRGSEERPETAMLYRPDTAESLLPPRRELPFHRPSISPPSGRDSVRSSSRPLTGLMGPPPVPARVASLRPSSSPENIRQSELPPQSQPTFVGGVRPWMQAAQQSPSTPTEAYSAPMPAATSLDNETKTRQSQSSSPVSSPLSLNRPSSNMHTSLRPLSSLGSAAQNPFRTTSQNRPATPPTSDVTYPEAGTSQAAHSSALKPDNNLAAYAMQSEEGRRVALNEFIFRHLESDEFLTLVEDMETCWARVTLGRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDU3</accession>
    <name>A0A9W8YDU3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Histone H4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">HHF1_2</name>
        <name evidence="13" type="ORF">N0V83_002306</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the histone H4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDU3"/>
    <dbReference type="OrthoDB" id="3919494at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22912">
        <property type="entry name" value="HFD_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000007">
        <property type="entry name" value="Histone H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035425">
        <property type="entry name" value="CENP-T/H4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001951">
        <property type="entry name" value="Histone_H4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019809">
        <property type="entry name" value="Histone_H4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004823">
        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10484">
        <property type="entry name" value="HISTONE H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15511">
        <property type="entry name" value="CENP-T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00623">
        <property type="entry name" value="HISTONEH4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00417">
        <property type="entry name" value="H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00803">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00047">
        <property type="entry name" value="HISTONE_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="10 11" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA box binding protein associated factor (TAF) histone-like fold" evidence="12">
        <location>
            <begin position="28"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4375220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="103" mass="11297" checksum="3F70002E709BBFCD" modified="2023-11-08" version="1">MSPGGKGGKGLGKGGAKRHRKILRDNIQGITKPAIRRLARRGGVKRISAMIYEETRGVLKTFLEGVIRDAVTYTEHAKRKTVTSLDVVYALKRQGRTLYGFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE61</accession>
    <name>A0A9W8YE61_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DJ-1/PfpI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002697</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE61"/>
    <dbReference type="OrthoDB" id="543156at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052158">
        <property type="entry name" value="INH-QAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43130">
        <property type="entry name" value="ARAC-FAMILY TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43130:SF3">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR RV1931C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1/PfpI" evidence="2">
        <location>
            <begin position="16"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="304" mass="33442" checksum="BBF9F26532C1249D" modified="2023-11-08" version="1">MSAIADSTEGRVEQVKVLFALFPGYNTLDVAGPLEVLSRSLQDPKDKSSRAFECQFVGTAAAMTSVQGLTIKADMDYEDANDELEDFDVIIIPGGEGVFDVLKNKSEPLGLLTKYVELQEKNPAKERTILAIDVGALLLAQQGMLEGLAATTHPDYYVRLETLCQDAARRDMSMRTDVMEERYVVNNARFDLGENPDDNPYIIKKSRQPPAEEEGPNTQHARRKSIARKGSNAWRDSRRRESIARRAQMPLGGMRVITTGGVTAGLDASLYLVGSLASHDSALEVARVMQYSWVKGVTVDAIDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEB0</accession>
    <name>A0A9W8YEB0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Hexose transporter hxt5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">HXT5_2</name>
        <name evidence="11" type="ORF">N0V83_001946</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEB0"/>
    <dbReference type="OrthoDB" id="5141738at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000044">
        <property type="entry name" value="Hexose transporter Hxt3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF92">
        <property type="entry name" value="LOW AFFINITY GLUCOSE TRANSPORTER MSTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="219"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="339"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="403"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="441"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="469"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="52"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="538"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4374868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="565" mass="62236" checksum="A87D64BC16E8772D" modified="2023-11-08" version="1">MRFFNRKQEVVHEKGTPETNTPIDTPARGNSHVGAAHDVEPKGRVPAIAVILGAVASIGGFMFGYESGQISGFLAMSDFIERFGENGEFSAVRQGAIVGLLAVGTLFGCLCSAPLADTYGRRLTISGSAFFYIIGVIIEITSERVWVQFAMGRFAAGLGIGALSTVVPMYQSESIPKRIRGATVSSYQLFITLGIWTAYMVNYGTSKDYFNSAQWRIPNGLSALWAIILGSTILLLPESPRYAYRKGKIEDARKNMARLNGVDPHSAFIDKEIAEIQEKLEAESTGGDHPWYEIFTGPRMLYRTLLGMVLQAGQQLTGANYFFYYGTTIFAATGLQNSYVTSIILGTVNVAATIVGLWIVENVGRRKAMMGGAAWMAACLFIYSFIGQYQLNHDDPTSTPQAGNIMIVFTCLFIAAFATTWGPLVWAIVGELYPARYRATCMGLATASNWLFNFLISFFSTMITNQIDYFYGLVFAVSLIFLFFIVYFFMIETKDRSLEEIDTMYMLHVNPITSAKWDASSLQKDGLVDTDRLQLGPGGRNFSKAEQSGQSGGILEPAERQEIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YED4</accession>
    <name>A0A9W8YED4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TMN2</name>
        <name evidence="8" type="ORF">N0V83_002265</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YED4"/>
    <dbReference type="OrthoDB" id="1666796at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF111">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041021511" description="Transmembrane 9 superfamily member" evidence="7">
        <location>
            <begin position="27"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="345"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="452"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="507"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="535"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="575"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="614"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4375181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="645" mass="71788" checksum="B4A56877D1097B5B" modified="2023-11-08" version="1">MRRLDISTCGAALASLLALAPSPASAFYFPGVAPTSYKTGDAVPLYVNHLTPADSQNDPKLRSVFSFDYYHPPFHFCQPKDGPKYIRESLGSILFGDRIQTSPFELNMGVNETCKPLCEAPYEGKDAAFVNARIFQGYDLNWLIDGLPAAQLLRDPSSDQPFYSPGFALGLMDADTPTLNNHYDIVIDYHEAGPDNLRVVGVLVDPYSMRDSKILGDGKATCGDGQTPLTLNENVNNQVAFTYSVYWRPSPTPFATRWDKYLHVYDPKIHWFSLINSAVIVVFLVGMVSTILVRTLRKDIARYNRLDQFALEDFGENGDVEDGVADDSGWKLVHGDVFRPPKNPLLLSVLVGNGAQLFAMTALTIVFALLGFLSPSNRGALGTVIIIFYTLFGFFGGYVASRTYKFFHGESWKLCFFYTPFALPGLVFATFFLLNLFVWGRGASGAVPFTTMLVVVIIWFIISVPLSFAGSWLGFKQAVIEAPVRTNQIPRQIPPAGGYLRPLPSMALAGVLPFGAIFVELYFIMNSIWFSKVYYMFGFLFICFGLMIITSAAVTVLMIYFLLCAENYHWQWRSFFTAGASAAYVFASCLLYWIKDVSFLSWTSGVVYLGYSALLSSLVFVLTGTIGFFASWLFVLKIYKSIKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEN7</accession>
    <name>A0A9W8YEN7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Laccase, multicopper oxidase, benzenediol:oxygen oxidorectuctase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">lcc1_2</name>
        <name evidence="8" type="ORF">N0V83_003518</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEN7"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13880">
        <property type="entry name" value="CuRO_2_MaLCC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13901">
        <property type="entry name" value="CuRO_3_MaLCC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000045">
        <property type="entry name" value="Laccase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF71">
        <property type="entry name" value="OXIDOREDUCTASE TPCJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="7">
        <location>
            <begin position="2"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="5">
        <location>
            <begin position="103"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="6">
        <location>
            <begin position="321"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4373225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="483" mass="52303" checksum="C84E660C91D2261F" modified="2023-11-08" version="1">MLVVNGQYPGPTIVADWGDDLEITVTNSLQNNGSGIHWHGFRQLGTNEADGVNGLTECPVAPGDTTVYRFKATQYGTTYGDGLVGTMIINGPATANYDIDLGVLPFTDWFYSPIFTVNAASLHARGPPTADNILINGTMTSAYGGQYAVTTLTPGKTHLLRLVNTGINNYIHVGLDGHPFTVVSSDFTPIEPYEATSLVIAVGQRYDVIINATEAVDNYWLRVGTGGGQCDGPNNNAANTRSIFRYAGAKAENPSNSSGVALPTGCYDETNIVPHQKTTVPQQMPEELKVGFTDTAGNGNLVQWLVNGTAMTVDLDHPTLQGVVDGNSSFAASRHVLSVGAKNEWQYWVIQMDNATFSPPLPHPIHLHGHDFYVLDHQENAVWNGDISRLKMDNPIRRDTATLPAAGYLILAFESDNPGAWLMHCHIPFHIAAGLGVQFLERESEIVSSNGDLGPMEQNCASWTKWREEYYPNGILILGDSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEQ6</accession>
    <name>A0A9W8YEQ6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_002136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEQ6"/>
    <dbReference type="OrthoDB" id="9970095at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1240">
        <property type="entry name" value="DOPA-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023389">
        <property type="entry name" value="DOPA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014980">
        <property type="entry name" value="DOPA_dioxygen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36423">
        <property type="entry name" value="AFR070WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36423:SF2">
        <property type="entry name" value="AFR070WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08883">
        <property type="entry name" value="DOPA_dioxygen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143410">
        <property type="entry name" value="DOPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4375056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="171" mass="19771" checksum="2770C9FABFF1D8C9" modified="2023-11-08" version="1">MADPSLYTYPSPLEGYQGLEPLPNEKALDGKSFVNPPAEKQSEAYNTFVAPITNGIRGGFDVHIYFLQTDTEEVKFATELWERIRREFPELRIYRVWDRPIGPHPVAMFEVNLFTPAQFGAFIPWLVIHRGPLSALVHPNTDEEERDHTQRATWMGQPLPLHLKLFKQRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF34</accession>
    <name>A0A9W8YF34_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YF34"/>
    <dbReference type="OrthoDB" id="3945550at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="442" mass="49703" checksum="E838C9DC58152880" modified="2023-11-08" version="1">MELATSPSLYRLKATCAYQDSDGDFDFNLDAVMELAAGLAPNLKEVTILDLFPNLSSRQRSVRKPLWQGLPGSTGKGIGSLTSLSLNGYSGFTSPAKLQNWAKHTDFDNLQHLALGGSYLTKSCGLSGEIMEWIARNHSFPHLKTLGVYLTRDDHYNERPHYSGNAISFFQAIDSLEELSINGPMEPQIIDAVLIHHGQTLKKLILNPFEDMQVFGNGRDRREIPMEFTKDRILQIQAQCPVLEELTITVMRDKSSASETEIYKCFGKMKNLRSLFLTLDCANWRIGRDPTYDPQFDGEDQELAWSYTNNVKRGNVREALINSAVDEALACSIWEMIIQNKTGRRLERLKLWTNDGGIFASTTPVHAVKTATRNVDAAESKVRRALRTESLGNDVKKRKWSITPPEEIWSSDEAKYTKQEEKATRAKCAKQEGSLHALSNHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YF80</accession>
    <name>A0A9W8YF80_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="4">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ACF2_1</name>
        <name evidence="13" type="ORF">N0V83_002434</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 81 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4473401at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052861">
        <property type="term" value="F:endo-1,3(4)-beta-glucanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.420:FF:000008">
        <property type="entry name" value="Endo-1,3-beta-glucanase Engl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1170">
        <property type="entry name" value="glycoside hydrolase family 81 endo-[beta] glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005200">
        <property type="entry name" value="Endo-beta-glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040720">
        <property type="entry name" value="GH81_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040451">
        <property type="entry name" value="GH81_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983">
        <property type="entry name" value="ENDO-1,3(4)-BETA-GLUCANASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983:SF0">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17652">
        <property type="entry name" value="Glyco_hydro81C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03639">
        <property type="entry name" value="Glyco_hydro_81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52008">
        <property type="entry name" value="GH81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="303"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="332"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="355"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="418"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="12">
        <location>
            <begin position="33"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="510"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1286"/>
            <end position="1587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1292"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1334"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1393"/>
            <end position="1402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1516"/>
            <end position="1537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1538"/>
            <end position="1563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4375348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1587" mass="174662" checksum="C669C80D8C8D8E2D" modified="2023-11-08" version="1">MMTPEDKVGGGGGGGGGGAGSEQEGHVLELAASYTPDEERRVLRKIDRTILPLMCLVFFMQVFGLLTDLNLTSSQYSWCSSIFYVGQLVSEYPAIYLMSKLSLTKFVGVTVVVWGVVCMCLSAPKSFAGFAAVRFLLGFTEGAVSPAFVTITAIWYQRSEHATRTALWVTMNGIAQVFGCLLMYGIGKNSSLALAPWRTMFLVCGALTAASGALFFWLMPNGPRDAWFLNAREKEVLSLRMAKDREGGDKTSFSVRQLREALMDAKTWCVFAFGLLVTMQSPVLTFASLVIKNIGYDEFQTMLYTAPSGCVQVLLLWIGVVGCWLLPRNRTLVVLALIIPPLIGSVLLLELSLSAGWGMIAASWLASCITAVMSPLLSLIASNVKGNTKRAFVNAVFFIGYCTGCIAAPQLWTHKPRYFNGVVTGIVTWCLLFVVVVAYWMICKRENVKRDKLTMRDGENGRGGEEGVSVDEFVLDKNGAPITDLTDKEDIRFRCLSLCYRSILTELPPGGPTQNPVAGPSETEANAPLRPAETPLAAGPTESRGQPSANIFQPIETGTPAPSLPFQGGHIVPKNFIADGDVPIETNKFYANWFLGSQGFPVWTHPYSLTWAKGAGNTWGMGISHIERDKFAFGGEPSGSTTPRYFIAPLGIYSIVLSAAELGRSTYLSVESLKAFSVYANLAASQTSRVLMSIPVVQGMGMVTAIYDRARPVLNTGVFFRTLDYMRPVNDASVVTHKFRIGLNDGTSWILYATPSTGQGVPPFILVNSTTIAGPADFVGMVQVTKNPSGADGESVFDSSAGAYAVNAAIAGSVNGGSGSYSLTWTKGGVKRQTLLMYALPHHVESFDQETKAALRNITLVTTTKGYARAVQADRITMVENDLPDTIGFAPWAPYTNGAAGGSENIGLNSGALALVNQAGIAELSENMDSQTRLNSMYYSGKGLAKFAAIIYTMNTMTGNRQYAAAGLNKLKDAFNVFVNNTQPEPLLYDTVWKGVVSSATYQPPYDTGLDFGNTLYNDHHFHYGYFVWTAAVIGHLDPTWLDEGINKAWVNSLARDYASPVTDDYFPFSRSFDWFHGHSWAKGLFESGDGKDQESTSEDAFATYALKMWGRIIRDANMEARGNLQLAIQARSFRNYFLLTSDNQNQPPSFIPNKVTGILFENKVDHTTYFGANTEYIEGIHMLPLNPSSAYTRSKEFVQEEWDTYFSKGRANLAQKGWKSILYANLALTDPQTSYNFFADPNFDTNDLDGGASRTWYLAYSAAMLNAGTASAEADGSMYYDSSAYVQSQDTSDEDLQDQSEDQYEDQPEDQTEDEYDAQYYDQPEDQSEDQSQDPYQDPYQDQSQDQSQNESQNQYQSQSEDQYQDQDQSQDQSQDQSQDTSQDPYQEESQDQTQDQTQDQTQDEQSEQVTGGVDGEGWPRGFNWPKKVGLETQSADTQDEDAQSEDTQNQDVQDQDDENSDQQPSQYFSFTYSDSTPQTQDTEDQNTTDGTDSSDMDWAMQDKVGDDAADTDSPDGNWSSYDDSSTQPSDTTVQSQEDEQPAADDDNDDDWEYDIDCDDGNENNTESQGEVVAPDTWWSANWQTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFM0</accession>
    <name>A0A9W8YFM0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA primase</fullName>
            <ecNumber evidence="9">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PRI1</name>
        <name evidence="11" type="ORF">N0V83_001130</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFM0"/>
    <dbReference type="OrthoDB" id="19606at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04860">
        <property type="entry name" value="AE_Prim_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.920.10:FF:000002">
        <property type="entry name" value="DNA primase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.920.10">
        <property type="entry name" value="DNA primase, PRIM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002755">
        <property type="entry name" value="DNA_primase_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014052">
        <property type="entry name" value="DNA_primase_ssu_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00335">
        <property type="entry name" value="primase_sml"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10536">
        <property type="entry name" value="DNA PRIMASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01896">
        <property type="entry name" value="DNA_primase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56747">
        <property type="entry name" value="Prim-pol domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3 9" id="KW-0639">Primosome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4375852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="499" mass="56825" checksum="0265403999EE7E0C" modified="2023-11-08" version="1">MPHSVTPESSPGDDSSRAISQADIIKTEPDTQDVAMEDAPTPPTAEKPKVNLEELFDDEDSDEEFPSSAPAIKEEELSQSAPIKITSKSTFSDPEIMRAFYQRLFPFRQLFQWLNHSATPSPHFAHREFAFTLPNDAYLRYQSFPTADLLRKQCIQMLPSRFEIGPMYSTNPRDRKTLRKASAFRPVMKELVFDIDMTDYDDIRTCCTGASICLKCWAFITMAIKTIDVALREDFGFKHILWVYSGRRGAHAWVCDKRAREMDDQKRRSIAGYLELLKGGDQGGKKVNARRPLHPHIERSLKILKEHFQTSILAEQDPWASEEKAAHLLNLLPDPTLKAALQKKWDSSPSRPSASKWADIDALAKSGALSKTSRDLLEAKQDIVLEYTYPRLDAEVSKKLNHLLKSPFVVHPGTGRVCVPIDTRRVEEFDPLSVPTVTQLLTEIDEWEGEAGTKKMQDWEKTTLKPYVDYFKRFIAGLMEEEKPVLKREREGGADAMEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFS6</accession>
    <name>A0A9W8YFS6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nucleotide-diphospho-sugar transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_001221</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFS6"/>
    <dbReference type="OrthoDB" id="3763672at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008630">
        <property type="entry name" value="Glyco_trans_34"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306:SF3">
        <property type="entry name" value="NUCLEOTIDE-DIPHOSPHO-SUGAR TRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="368"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="383"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4375942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="418" mass="47814" checksum="326B3F25A293C8CA" modified="2023-11-08" version="1">MLAAQRYYIAVVAVFLVVASCSFYLFRSDEPIAYEPVVRQPYRSSKPTIRDTITTLFESIKHDPTSPGYIDAAGNYFKGGEETVWTEPLGKRVLIVDIDTRIPTGENQILNPDKLDWEHLDMHGGGLVSSAILNHYFYALVHGYDYKFYQARHLTDLHDTWIMPHVFRELVPDYQFVIAMDADVTVSNLDVPLEWMFNRWGIKNHTSMALPWDTEEFRNNKSISTDDKGLLVLNTGFVVAQNSNTTLELLEAWRDCPKEERYEGCSRWAYEWSHEQRAFSEYIRYDFNSTPETIVSIPCDDAVGWPGFKEDVEGGNPGITDCNGNFVRHHTLGKSQVKHAGAASVMQALSEVLQKSILSQQETMWFKEPEKKVEEPPQNAELRDEEEDEEAETQGQEKKDDSDVSPVILENLITDIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFW4</accession>
    <name>A0A9W8YFW4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_000012</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFW4"/>
    <dbReference type="OrthoDB" id="2988756at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF146">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="159"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="352" mass="40192" checksum="9766DEBC78D80623" modified="2023-11-08" version="1">MSSRQNYITEDHLLNTTYGMLTLATIFVIARIVTTQLVRPKRWILQDAIIYLAFILYIVMAVLYILVTPILFRLTAVGEKRLKPYPTLQDEHKFMVRIFFVNSMMLWFILWTIKLSFLVLYKKLMEGLRDVYLKLWWAVTWFTPGACQTPRDVRFQIASLYYTYAVDVLTDIMIMALPMRLIWNLQMPRTQKFGVIALFAIGTILITIATLRVVQIGSKAQSSSQPSASWLALWGIIECSIAIVIGCCPSFVSLIRNRVSPGVSYNTQGFVRQREQGAQTDSNSPDSVKLKSVHTSVTKSNASNSDLGFIEADGSQFQLVNGGRDLVEDIVVERPEGRKKIRIRRGSCRMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGR0</accession>
    <name>A0A9W8YGR0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histone acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_000256</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-lysyl-[histone] + acetyl-CoA = N(6)-acetyl-L-lysyl-[histone] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21992"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:21993"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="2"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGR0"/>
    <dbReference type="OrthoDB" id="3361892at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032931">
        <property type="term" value="F:histone H3K56 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051236">
        <property type="entry name" value="HAT_RTT109-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013178">
        <property type="entry name" value="Histone_AcTrfase_Rtt109/CBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016849">
        <property type="entry name" value="Rtt109"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571:SF2">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE RTT109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08214">
        <property type="entry name" value="HAT_KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01250">
        <property type="entry name" value="KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51728">
        <property type="entry name" value="RTT109_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="349"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="502"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="358"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="378"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="502"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4377431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="583" mass="64706" checksum="FE2C8A4C3017FF6D" modified="2023-11-08" version="1">MADIDTSKPLKELLAEQLPKDVPFTFYHYSTPPSKSSALFSAPPHGKSERTYCESHTLAASIIPKDAASSELPEELLVLAIEVLIYTTRNLTTMFVSKADSTGYLSELQLPRSQSGSPLKAICGTFVAWLARERQREGKKLVVSLFARAQDQYLFPASIENKNKHVLDDRGLVKWWCRVLDPMIWQYQAEEERRPFAERLVEGTAAGTNGAPSQSKTTAKGYLVIPGFEPYDTLRYTPPPPKPNVPRRWATTHPLLQIAPHPAAPPRCLVPHFPDDPKTRFLEELDEELPDRGTDAMVAEGGTPSRSNGQWKSVKTLDQFWEFMAFRQECSSGRIVGFIWVVLSPPKPSIPEEDEDTPSQQSFSQPLSQIGSQEHVPSPKRRRRKRQRRDDKIRYGPIPLVLPKIKASSSNLSTTSNMSSNSNAKLPSETSPYYVWPASSRGTVCFSTKNYNRAHEVLLQQSFANRRAAAQSTKKWKEEVGVLGGLEKWDWTVTGKKENLAASSTATNGSSPNIVMGVRKKRKPDTPAEASSTLDTPTEGVQVLSEGLVRKKVKIEPTPVAEATVSNGVNTLSAGLVRKKSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGX8</accession>
    <name>A0A9W8YGX8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">GCR1-dependent translation factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GDT1</name>
        <name evidence="9" type="ORF">N0V83_000604</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GDT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGX8"/>
    <dbReference type="OrthoDB" id="442680at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015085">
        <property type="term" value="F:calcium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032468">
        <property type="term" value="P:Golgi calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032472">
        <property type="term" value="P:Golgi calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001727">
        <property type="entry name" value="GDT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049555">
        <property type="entry name" value="GDT1-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12608:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12608">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN HTP-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01169">
        <property type="entry name" value="GDT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01214">
        <property type="entry name" value="UPF0016"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040958063" evidence="8">
        <location>
            <begin position="23"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="321"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="427"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="468"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="502"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="46"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="348"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="85"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="123"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="185"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="352"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="395"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4377774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="525" mass="55739" checksum="56E282326589E50D" modified="2023-11-08" version="1">MRLRPSQTHTLLLLLPALRVLATSIAVDTADKPVARAAVPAYTLASSLPDASPTPASPGSKGTKDAPVDGLDGKPHAGPYVDDTPTSHDKKPNVVEDLRPGKTPASSLEDSTTLVEADDSVLDGDKSVMQDPDRKLATGSTGTEGGVSAKDKERLAHEEKTGEKMEKVPESPKEAPPQPHADQKQLGNSPMTTSTTRVLGAVGLEKPTGLPDTPHDVPHPVPGLHDDTDPLDIIPKTKTPASLPDEDVGGGVIQPFHSFVLSFTMIIFSEIGDKTFLVAALMAMRHPRLLVFSAAFSALVVMTVLSAVLGHAVPALLNERFTHFAAAALFLVFGIKLIREGLAMSPNDGVGEEMREVEQELEEKEQLARHQKGRRTSSISPYALEAGRARRSRSNSRLPAPARSPSTSPDRSPSPNRTSVASAMGGLNNLFSLLLSPAWVQTFIMTFLGEWGDRSQIATVAMAAGSDYWWVTAGAVVGHGLCTAGAVIGGRAIAGRISMRNVTLGGAFAFLVFGVIYLLEAFYHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH23</accession>
    <name>A0A9W8YH23_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TFIIH/NER complex subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TFB3</name>
        <name evidence="3" type="ORF">N0V83_000780</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH23"/>
    <dbReference type="OrthoDB" id="5963at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015877">
        <property type="entry name" value="MAT1_centre"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12683">
        <property type="entry name" value="CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12683:SF13">
        <property type="entry name" value="CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06391">
        <property type="entry name" value="MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MAT1 centre" evidence="2">
        <location>
            <begin position="81"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="317" mass="36232" checksum="EDE39EBB60EAAD4D" modified="2023-11-08" version="1">MSKQPARENGASTKRLAEDGGASPLSKSLHSLSLQQSARSRSLADPSSDICPVCKSSRYLNPNMKFRVNPECYHKMCESTLRRNKFREQTFEDLKIEREVDIRKRVMSVMNKSEADFETLRDYNDYLEQVEEITWNLILKIDVEATENRLRRWEEHQKAELNQVPSRREPEDHAIKKGAVQRKAPNASSGNTPDPFASKDDQDTGFSFRGLKKRVAPPKEAPFDPFDGWEISPQYYVLQDNYEVDWYKTYKQDTSHLVAMWDVRDYASRTLREAFGGFGIFIEDEINARSVPSGNASIGTQQAAIAAGSDVNMDDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHT8</accession>
    <name>A0A9W8YHT8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable glucan endo-1,3-beta-glucosidase eglC</fullName>
            <ecNumber evidence="5">3.2.1.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Endo-1,3-beta-glucanase eglC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Laminarinase eglC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">N0V83_001026</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Glucanases play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. This enzyme may be involved in beta-glucan degradation and also function biosynthetically as a transglycosylase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <topology evidence="3">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="5"/>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHT8"/>
    <dbReference type="OrthoDB" id="77201at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000233">
        <property type="entry name" value="Probable glucan endo-1,3-beta-glucosidase eglC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050732">
        <property type="entry name" value="Beta-glucan_modifiers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631:SF13">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE EGLC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0134">Cell wall</keyword>
    <keyword evidence="16" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 21" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040912362" description="Probable glucan endo-1,3-beta-glucosidase eglC" evidence="21">
        <location>
            <begin position="18"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00025152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00032134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00032906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ4378192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="310" mass="33509" checksum="A3EB6E0913CE65EA" modified="2023-11-08" version="1">MWTKAASLLALVSTAIAQSKGFNYGSTNADGSCRGYNDFVRFFNEAKSLPGTSGFTAARLYTSIQCGTTADPIEAYRAAIDTDTSILVGLWASAGRDVYQNELSALIRGANELGTAFTDRIIGISVGSEDLYRSSPQGVANNAGVGATGAEIEGYIGWMRDWIKGTLLESKPIGHVDTWTAWVLPENRGVINTVNWLGHNSFPYFETTKPNAIEQAAENFWSAVGQTESVSGGKQVWVTETGWPHVGPVSGSAVASLDNAKRYWDEVGCSLFGTRNTFWYTLKDANTAQTDLSFAVTPLENNTPYFDLTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHV3</accession>
    <name>A0A9W8YHV3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_000011</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHV3"/>
    <dbReference type="OrthoDB" id="2151789at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF34">
        <property type="entry name" value="FAD BINDING DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_3G02770)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040725316" description="FAD-binding PCMH-type domain-containing protein" evidence="5">
        <location>
            <begin position="19"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="6">
        <location>
            <begin position="95"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4377188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="536" mass="58481" checksum="20F332D4C6A97299" modified="2023-11-08" version="1">MRISSVFALGFMPAGIWAQDIFEPADFNITQALLKNGVDVSGIPELTGLSERSLSSGCSIACTSLKVIFGDAKVESQDETAYDDVINYFWSTQQREPKPYCIFKPEKSIDVSTLVLLSRLTQCPFAVRSGGHAAFAGASSADGGITVTFEKMKQIKLSGDKKIVSIEPGNLWYDIYTTLAADDLAVVGGRVSGIGIGGLTTGGGISFFTNEYGWACDNVASFEVVTASGIIVNASPTSYPDLYWALRGGGNNFGIVTNFNLNTFAHGPQMFGGQRRFFNTSFPAAIDAFVNLGVNAAQDPKAAQFLAITMGASTGTTVAIAELEYADPVLDAPVFEEYRNIPAFSDTTKLDTLAYFTQQLNNSNPNGYRETFWTASSKLDSQMVQFVFDVSFEEFSKILDVQGIIPANTLQIISVPTLEQMQKNGGNVLGLKPGDGPVFLINISMRWDKTADDARVLKASANVVQRMLDEAKRRNMVNDFLYMNYASQFQDVIGSYGESTQRLKEFAKKYDPTEVFQKLQPGYFKLDGAPVQSWPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YI99</accession>
    <name>A0A9W8YI99_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flavoprotein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000848</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HFCD (homooligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YI99"/>
    <dbReference type="OrthoDB" id="70387at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF21">
        <property type="entry name" value="FLAVOPROTEIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140860">
        <property type="entry name" value="Pseudo ankyrin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="2">
        <location>
            <begin position="12"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4378018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="580" mass="66320" checksum="8BCD1EEF9B12899D" modified="2023-11-08" version="1">MPPQSPERPLNVLVALTDPSAFRFGCSVVARLHRLHHVSLKVIACNDFPKSTHSAVPSQLEPVWHTYDAENNTTPGWQHADFLETKVAEYCSWADLLVLAPLDAENVARMLHGFTSNLHLQIMRSWHVSKKVLLIPAMSTLMWENPMSRKQISKIRRKWNWIRVLPPFLWSFDPAGRKYQPWEGHKAFDESIQTQIDLMAVGHRTVALPIAHLPTLTPTRSKGARLPPELWSIIMEFTEDWELSTTLNVHTNLPVPPEWRQSASEEGPKTQMQKLEWTLLTCPYSEIKKLLDLESPTRISRLCIELIMRFAMVSVLAHLENYHKDLFWGTFGHAFLPHKASVFGKTEILEYWRTSPTFLTKEYSAEAIDNASRTNSVQALGWWHDSGLPLKYTEAALEQASSQGHIQVLKWWKEHSRYRTAPTSPPSPSEDKDTSKAHRELLKLKVGKSITYATQSGCLPAIHFWVESGIPFSHEDTIAKIASHHGHTHILDYWRKLRGEKMLFDNQVLVGPTKMGHADVLAWWKRSGLRVEYKTCDIEEALEDGVEGERGESVRRWWALNGLNLGVGTSEWMRVKVLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJG5</accession>
    <name>A0A9W8YJG5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">3-hydroxybutyryl-CoA dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_000888</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJG5"/>
    <dbReference type="OrthoDB" id="5958943at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000009">
        <property type="entry name" value="Fatty oxidation complex, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022694">
        <property type="entry name" value="3-OHacyl-CoA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006180">
        <property type="entry name" value="3-OHacyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075">
        <property type="entry name" value="3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075:SF5">
        <property type="entry name" value="3-HYDROXYBUTYRYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000105">
        <property type="entry name" value="HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00067">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase NAD binding" evidence="7">
        <location>
            <begin position="35"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="6">
        <location>
            <begin position="224"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="40"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="4">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000105-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000105-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4378058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="34186" checksum="963A4A04562E1369" modified="2023-11-08" version="1">MFSSASRQIARCAARQQCRTFSSTPSVGAAAEVKKLGVIGAGQMGLGIALVAAQKAGVPVQLIDNSQASIDKGLKFADKLLEKDVGKGRISKEDAAATRERLTSSTKLEDLSSVDFVIEAVPEILDLKHRIFSQLAQICPSHAILATNTSSISITKIAASTTKDPTDLSASSRVISTHFMNPVPIQKGVEIITGLQTSQDTVDTAIAFCERMGKIASKSTDSPGFLANRILMPYINEAVICLETGVGQKEDIDSIMKNGTNVPMGPLQLADFIGIDTCLAIMQVLYHDTGDSKYRPAVLLKKMVDAGWLGKKSGKGFYDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJP1</accession>
    <name>A0A9W8YJP1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJP1"/>
    <dbReference type="OrthoDB" id="203796at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37848">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37848:SF1">
        <property type="entry name" value="SUN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4378178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="411" mass="46694" checksum="8A6DE8709A0C4ECA" modified="2023-11-08" version="1">MGRASRTGVAEGYRDDPDAVSLHTTPDDYTYDDAPEITGLPPSYADSEADSSAAAVVPIRHVPPPTTRTDHNNPGFRNGKPVVCETTTYMDPRYDTDPVFLEETIRSCAQSAPVPLVYIMGTHKETIKKGDKKETREITDFRIVVNLQRYLRRNFDDRDFSSMELVTVENGEKTHRGTITKVRAPGAKQDIEVGTQKATLTEWCHRYCASPRMLRTFRLRRIITGFDEVQLKNRIEGVIRATNYRGHINITFPIEDKNVDIYTSNRINQWRLKNWICWIFYLTFLWIFTWPYLFFATKRYAIVRAEWPFSFTDGHGNKTYTTVSEEQWFEKWHVAIRRLALDRYQGEASEEMMNGVIARPADPPMPGTVSTGHAGVDSAMGLLTQGFQVARAIQGGGNLGRGLQGGLGYDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YK22</accession>
    <name>A0A9W8YK22_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Succinate dehydrogenase subunit C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_000770</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YK22"/>
    <dbReference type="OrthoDB" id="588261at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03499">
        <property type="entry name" value="SQR_TypeC_SdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018495">
        <property type="entry name" value="Succ_DH_cyt_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014314">
        <property type="entry name" value="Succ_DH_cytb556"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02970">
        <property type="entry name" value="succ_dehyd_cytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10978">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE CYTOCHROME B560 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10978:SF5">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE CYTOCHROME B560 SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01001">
        <property type="entry name" value="SDH_CYT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4377940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="19060" checksum="F883FA2A87D0D73D" modified="2023-11-08" version="1">MASQRIFQLGLRRAAAPGLRVQPAGRIGQRRLAATQAEASEILAKQRLQRPVSPHLTIYKPQITWYGSALNRLSGIMLSAPLYLFGFAYLAAPTLGWHLETQSMAAAVAGWSATSKILLKSYFALPFFYHSLNGLRHLSWDLGTGFKNQTVIRTGWSVAALTVASSVYYIFMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CGC9</accession>
    <name>A0A9W9CGC9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA binding protein, heterogenous nuclear RNP-K like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">HEK2</name>
        <name evidence="5" type="ORF">N0V83_010919</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CGC9"/>
    <dbReference type="OrthoDB" id="1937934at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22455">
        <property type="entry name" value="KH-I_Rnc1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22456">
        <property type="entry name" value="KH-I_Rnc1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22457">
        <property type="entry name" value="KH-I_Rnc1_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049786">
        <property type="entry name" value="Rnc1_KH-I_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="43"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="129"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="280"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="222"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4361978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="362" mass="38224" checksum="F0720152E915AF25" modified="2023-11-08" version="1">MSSEESQAGPGQDANRVAEQLDRLAIDGEGDVAPRTEQEYAESQLTLRAIVSSKEAGVIIGKAGKNVADLRDETGVRAGVSKVVQGVHDRVLSVTGSLSGISKAYGLVAKGLLEGAPAMGMGGLIRSDGTHPIRLLISHNQMGTIIGRQGLKIKQIQDASGVRMVAQKEMLPQSTERIVEVQGSPSGIEKAIWEIGKCLIDDHERGYGTVLYNPAVRVQPGVGPGPLSNGGATGTPGGRSYNRTGHGADFSDSPPTYSRRSGSDAASRPPPPTHTEDGEEMQTQNISIPSDMVGCIIGRGGSKISEIRKTSGARISIAKAPHDDTGERMFTITGSASANEKALYLLYENLEAEKMRRSQAQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CGN4</accession>
    <name>A0A9W9CGN4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protection of telomeres protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_010566</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the telombin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CGN4"/>
    <dbReference type="OrthoDB" id="2186770at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000783">
        <property type="term" value="C:nuclear telomere cap complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098505">
        <property type="term" value="F:G-rich strand telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010521">
        <property type="term" value="F:telomerase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032210">
        <property type="term" value="P:regulation of telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016233">
        <property type="term" value="P:telomere capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000303">
        <property type="entry name" value="Protection of telomeres protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028389">
        <property type="entry name" value="POT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032042">
        <property type="entry name" value="POT1PC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011564">
        <property type="entry name" value="Telomer_end-bd_POT1/Cdc13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14513">
        <property type="entry name" value="PROTECTION OF TELOMERES 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14513:SF0">
        <property type="entry name" value="PROTECTION OF TELOMERES PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02765">
        <property type="entry name" value="POT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16686">
        <property type="entry name" value="POT1PC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00976">
        <property type="entry name" value="Telo_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Telomeric single stranded DNA binding POT1/Cdc13" evidence="10">
        <location>
            <begin position="6"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="337"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="361"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4362473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="600" mass="68496" checksum="1F43B85E5B824C09" modified="2023-11-08" version="1">MPPHGFTAIKDATGHGNAVSLIGVVVSIKEPRKSRGSDWVLEFAIQDEFDNTLVGGSSSINCRIFRSQDRLPKISGTGDIVILRDFKLSSWGMRCDCISDSRLYSGVLVFPANKIPIPELSQAYQAGSQRLLYTATHGTRDPTPEEQMTVIHLKHAASGSVQQVRQHATTVSYNASARDRSSLIQGLCFDKFYDVCVQVVNTYYTLHSTVELKVTDYTSNENLFYYADPNEEDAFMVTNKNWTGPYGQLTLNVTLYESNATWARENVSIGDYIFLRNMRTKLSQRDKLEGIVHQDPQKPSQVDVRRLTNQKEIAEITKRREAYEKQRGTKSALEVLHNAPNEPFTESTLSKKDRKKLKLKQRQEKKEAELKELEQKEEKWEAERSGINTNIRAAFPEVKLSTISEILYSPRLNTRTPKYNNYKLPFVNAKHRARVRVVDVFPPELERFAHALNDPTWNRKANKHDPNNNSTKVRWEWGFVLLVEDANVPPNTVSEKLRVVVGNDVGQGLLDLNAVDLKNNPEIFKKLTERLFILWGNLYELKTELRDRGSDLPLPPGDNRLQNKAFDCCIEEYGHEVPVSETHPEGYQRMHRLAQTFITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI50</accession>
    <name>A0A9W9CI50_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">MFS general substrate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_008627</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI50"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000934">
        <property type="entry name" value="Allantoate permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000064">
        <property type="entry name" value="MFS allantoate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF70">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="327"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="381"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="448"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4365011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="514" mass="56591" checksum="CCE50F71BA56D922" modified="2023-11-08" version="1">MATSTSTSLNNKDIGVIHPSPPIPSSETSSRYDDTYEAYKNNQHLQTDPVEAKKTLRKIDFRIVPILFFTYLLQYLDKNGINYASAFGFIEGTKLGKNGYQWLGSIFYFGYLVGQWPAGYLLQRLPMAKFLGCCTLGWGIILMTTPACHNFAGTAANRFLLGFLESTVNPGFVLIMSMWYTSAEQPLRLEAYYCTNGVATMFGGLIGYAVGHITTGLPRWMYVFLIFGSASTVMGIFTILFLPDLPTTASFLTERERIIAVERVAGNRQGVKNSHFKKYQAWQTLRDPKTWILFVMATGAQIPNSALTSFSSIIIKSFGVDTLGTQYLQIPGGAVQFLSLLVGGFICTHYPNSRCVTMCVANLICIVGAALLVGLPSSNKLGRLVALWMCYFQGLGFSMSLTIVSSNVAGTTKKQLTGAVLFTGYCVGNIIGPQTFRDSEKPGYRSAYVAMLVGYTIKLIAVLVLFGYMWSVNKKRDAESASGIRLTDEEEKAAVEAGMLDVTEVDNKGFRYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI59</accession>
    <name>A0A9W9CI59_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Platinum sensitivity protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PSY2_2</name>
        <name evidence="6" type="ORF">N0V83_009967</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI59"/>
    <dbReference type="OrthoDB" id="27483at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030289">
        <property type="term" value="C:protein phosphatase 4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072542">
        <property type="term" value="F:protein phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055236">
        <property type="entry name" value="EVH1_PP4R3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006887">
        <property type="entry name" value="P4R3-like_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051137">
        <property type="entry name" value="PP4R3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23318">
        <property type="entry name" value="ATP SYNTHASE GAMMA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23318:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22972">
        <property type="entry name" value="EVH1_PP4R3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04802">
        <property type="entry name" value="PP4R3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PP4R3 EVH1-like" evidence="5">
        <location>
            <begin position="11"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Serine/threonine-protein phosphatase 4 regulatory subunit 3-like central" evidence="4">
        <location>
            <begin position="140"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Serine/threonine-protein phosphatase 4 regulatory subunit 3-like central" evidence="4">
        <location>
            <begin position="376"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="648"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="717"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="660"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="720"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="730"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="775"/>
            <end position="785"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4363671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="823" mass="92473" checksum="D42EFE034EE4EA48" modified="2023-11-08" version="1">MALAPPPASDRKRVKVYELKNNDWFDRGTGFCRGVLANQEEARIVVLSEDDQTRQLLETRISKDDGYQKQQDTLIVWTEQNGTDMALSFQEPEGCASIWDFVNDVQSRLQALAQDDGLSDDIEHISPILLPNPDLGNLHEIENHMRAANSTPGGREALAKFILAQNYIPKLIPLVDMAEDLESVSDLHRLCSIMKLLILLNDTSIIEYVVTDSVVLGVVGALEYDPDFPLHKANHRQYLADESKFKEVVRIEDDSIKRKIHYTYRLQYLKDVVLARILDDPTFSVLNSLIFFHQVDIVQHLQGNGPFLKELFGIFGPKEQSLPRKKDAVLFIQQCCAIAKSLQANGRAQLYQNFISNGLLEVIQFALKHQDASAIQEKSKPLTDTLIELLLIEVDLGVKSQMADAIKILLDPNANSASIEALGRTNSDFLAKMRGQLPSIPQTDSFIQNFYDESARKLFQPLKDLEGRESMLDLSMQEVSLYAHLVEILCFFIRQHSFRSKYFILSEGLAARVAQLMDCPEKHLKLTALKYFRTVIGFHDETHNRQIIQHHLFEPILKILFDTMPRDNLLNSACLELFEFIKRENIKLLVQHLVETYREKLQGITYVDTFQNLILRYDQMHEPVTTQELDHSFTSVDSDTPARHAVVNGGRWGQGLKDMDPDEEAYFNTSDDEDEGVPSVGKPITNGASPVRPLVNYPDDDADEDDMDILAADVPASAHKMSTATQTPDEATSPESKPAASPPERISEKRRREEDEEDELLASIATSAGPKRRASTASNASTGSLRSLRRKSPNINAGKDGGTPKKISLHIPVKSSGETGEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIP9</accession>
    <name>A0A9W9CIP9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cysteine protease</fullName>
            <ecNumber evidence="11">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ATG4</name>
        <name evidence="14" type="ORF">N0V83_008370</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:67548"/>
            <dbReference type="Rhea" id="RHEA-COMP:17323"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67549"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase C54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="11"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIP9"/>
    <dbReference type="OrthoDB" id="2960936at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019786">
        <property type="term" value="F:protein-phosphatidylethanolamide deconjugating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005078">
        <property type="entry name" value="Peptidase_C54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624:SF49">
        <property type="entry name" value="CYSTEINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03416">
        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 11 14" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="13">
        <location>
            <begin position="167"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="15"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="114"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="405"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="518"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="411"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="518"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="532"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4365750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="544" mass="60094" checksum="01924ACEB059D018" modified="2023-11-08" version="1">MLTFVAARPTIRQAADDPDLAASQHRAPDDTAVSASSSSRQKNACSSLQRPSSRTAASASIAAFDPHGFSQAAMNDFERVGRTIVRTFYDPLPANDSNDPIWLLGQRYEPRPLLSKHASSDAPSTDATTPQTERTEDESWIRTSVDEQDRKESPNGQDPAQYGNWPSAFLDDFESRIWMTYRSGFTAIQKSQDPKATSAMSFRVRMQNLASPGFTSDTGFGCMIRSGQCILANALQVLRLGREWRYQQNPTAREHCDILSLFADDPNAPFSIHRFVEHGAAVCGKYPGEWFGPSAAARCIQDLVNKYKEAGLRVHVSGDGADVYEDKLTQIAVDDDGQWQPTLILVGTRLGIDKITPVYWEALKASLQMKQSIGIAGGRPSASHYFVGTQGNNFYYLDPHSTRPLLPYRPSPSTSQTGTTTDPSTLTSSTSSVTSTTSSTTIIPSASEAKTLTDRHSTTSYSVEELSTCHTRRIRRLQIREMDPSMLLAFLVTSKQDYEDWKKAVQSVQGKCVVHVQDREPAPRGQERAEAVDEVESWDEDGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIQ3</accession>
    <name>A0A9W9CIQ3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009035</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIQ3"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055530">
        <property type="entry name" value="DUF7104"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF78">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23397">
        <property type="entry name" value="DUF7104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="2">
        <location>
            <begin position="46"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="808" mass="92193" checksum="75DBB7142FDEE46B" modified="2023-11-08" version="1">MEKFEYDPIDLEESSFRLLRLLKGDDLHVECELFEAWLHGDSAFEFEALSYTWGSPYTCETITVNGKELAVTENLYLALRNLRYQNMDRILWVDAVCIDQRNNHERGHQVQQMGSIYSQADRVIFWLGLSTEPIDILMRSLKQQGRENYDRQNPTNRNMLANGFFAQPSTSEANSESVEKRYGGSQKHIEQQREGLRDLLTRPWFQRVWILQEVANARRAIVCCGSNEINARIFAHASQRFDFTPSSHTQAVLDIMPGPSGESSWWSQKRNLYTLLLKFKESRASDPRDKIYALLGISSDGKRAHGLRADYTKTTEEVIHDITSYLFNIDQSPYQVMSEFLNNFHALNTSSLVKGVQSTEVREIASFLQERGDLTSITEEVLQAAAANTRYGVEMVEFFIDRQDFTLKDSILQSAARNEGCGLQVLEFLCESHRPGVSSKRKQELDVVSKGTIEAAIANKRQGNKMLDFFLSHLEDKVSTELVLELASSNMDTALDIVQRLIEYRGDRVIMAAAGGAMNMMALVVEEYQRRKWQIPTEAFKVAAGNTMYGPEVTQLLLEQEHRDKITTNVLEAALKNRKCGDKILALHLQQKEVRSIPITQSLFLAALGAFCEHLILPLAIEQRRRRVTTVETPQEEECAVYRELYGECRFATVNKMEALARTYQEQGRLEEAAKLWRQVNDQKKVHFGMHQAHTRANMTELASISVEQGLLEAAEDLLVQVIAHEEEIVRFNGNLISGYTADTPVGSVKETLLRGREMLSLIREQKAARDLTAAAARDLTAARLSSWRRVVLRFRRFRHQSHPEVHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIZ9</accession>
    <name>A0A9W9CIZ9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009309</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIZ9"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="94" mass="11031" checksum="EDB6B6D849E7FB5B" modified="2023-11-08" version="1">MSRERERKAEAEAEAKAARKATIERGKMEAELEAARKAAREWKKIKAELEAARKPDKEREEAEKVAKKGLEMRKQMLRKAIAKQEKNLKRFRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJ16</accession>
    <name>A0A9W9CJ16_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Galactose oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008585</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4364969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJ16"/>
    <dbReference type="OrthoDB" id="205993at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040778675" description="Galactose oxidase" evidence="3">
        <location>
            <begin position="25"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="449"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="503"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="732"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="906"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1057"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="556"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="596"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="632"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="694"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="745"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="812"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="910"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1073"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1105"/>
            <end position="1115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1161" mass="125041" checksum="43A8B6C6E586D5BE" modified="2023-11-08" version="1">MRPFSMSLAVRVPWLALLLPITMAQMPYNPTRILQHDNLLYVFQPSSQSSSQFQLGSIDVSSHVVAANLPYTTLYPTLPFLDDDVLRAFTPILDNGGNLTVYTGDCTQGASGGQIWSFTPDSSEENGKGTWDKESVSVEDSKHVATMGANFLSGGIAFSSIVGGDVADTGAYVFGGMCPTLNETTDDWQSAANYSNLMVTLQPSSTETETTNYQLDLSSSRGPPIPEAGFSITGLTPTFSNRSDGTQTQQQNFVLVGGHTSTAFINMSQVALFSLPEQGWTFIGVQQPDSAQTKLATRAQVTSIDPRSGHTAVLSPDGRKIIVLGGWVGDINTPADPQYAVLNVADGYGGDGDWQWTVPSTSGVGLPDNSGIYGHGATMLPGGVMMITGGYSISSSPSGRKRATPTASTKAYFLNITSNTWTTDYSPPPQVSDSEPIKTGPLATPGQKAGLGIGLGVGMAAVCGLFGFYMWYTRRLKRQREMREKQIQDLAFGAHRYNIEDYSPGFDGRDGRSDPMDSLVGPNGSYYFPPGTQGGQGWRSSNGGDAERTGLLVEIPSPTRGLRRSLSGRPGYAMGRVRGSGHIHPIDELEEEPDEEHTHDKTPLAKQPEMTERRSDRGKSILDNAPVLDPFTDTRGRSEEQKSMYHSAPTTPNAEESGESDYVAANWLSGAPGTSGRHSPSPSGRISPDKSTSERTSSNLSERSARSNISWTSSSGGVIRTASMRASAILNAAHANPFKTPDASPTHDKQRRTSERGRQSPDDPRTQSFTSIRSSGWPDPDTFHTAHSSFAHLQAEGEVLLGGNPERVRPGTASSSSNTYRDTEGSNSRAATATPATSYALDSTTLSRPATRERRKSWLGSVRRVLTRSASSAERTRSMTTTTMSYHERYTDNPLSPIDTSVADNRKTFPAASSPPRRAASDASFWRNRRGKQDWLDDEQDPKWRRNAGDDWGAPEDIAMAERERLRQEWRDRGNLLVNIDHIDDQLPTPRTPIRSDQLGVPPPATFMEERPCTPASEADWDVEAAVERRVVQVMFTVPKSKLRVVNADVDRSSILSLPREDSREGDELLVGASGGGGEASGGGGGGTGSPSRVKDLAGRFEQLSSPSRMTSPRTSPRPSPSPSIKSMKIRAKASSASLGGKQKMQGGGGRRGSASSPLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJF8</accession>
    <name>A0A9W9CJF8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">tRNA(m1A58)-methyltransferase subunit TRM6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TRM6</name>
        <name evidence="8" type="ORF">N0V83_007450</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRM6/GCD10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJF8"/>
    <dbReference type="OrthoDB" id="10254665at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017423">
        <property type="entry name" value="TRM6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12945">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12945:SF0">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT TRM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04189">
        <property type="entry name" value="Gcd10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="271"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="495"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="601"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="308"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="337"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4366920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="623" mass="68487" checksum="4AFFBF9E3ADAEBE2" modified="2023-11-08" version="1">MHSYIRPNTHVALRLPSGIVKILEITPNQTINLGKFGTFNSNLILGRPYYLTFELLDKDHGKSKTELRVVPASELHAEALGNEHAPATEASDEAADSGAGFDIVSEDGQVLMRNNRLTVDDASRQALSMAEIEELKKAGAGSGKDIIAKIMASHNAIDEKTTFSLAKYTLRKSRKYLKRFTALPLDVGLLTEYILEKEAYKIMELREDLLGLICSWANIHCGAASQISTAEDGVSQIGGGRWLVVDDTGGLVTAALAERMGILYPEDENDALEEEEEADRSVQQDEPPQAPEQNGSDTVMADADKANTTESTSTTAAPSDPPPRPRKPPRPHIPQMSAPTNSLTVLHPNNQPNLSHLKYFGYDYGNPTPSHPLYKHLKTLSWLSMLHPEDDPQYDEPEVISEEVLATMKSSKRGTYYKKRRRWERVKSVVDETRRGGFDGLVVATAMDAKTVLKELIPLVKGGGKVVVYNPNVEPLVELCDLYSKERRAAYIAREFGPPPTQRQPEANGSSRSKKPHRIDEDDDAETSNESTASILGNEDAEDFPLNPTLLLAPMLQTARARQWQVLPGRTHPMMTSKGGAEGYLFTGTRVLPIEGRVEARGQHGGKKKRKVDEGGKGVVVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJH1</accession>
    <name>A0A9W9CJH1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">HTB1</name>
        <name evidence="16" type="ORF">N0V83_008371</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the histone H2B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJH1"/>
    <dbReference type="OrthoDB" id="10254238at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000014">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 13" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12 13" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="15">
        <location>
            <begin position="30"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4365751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="138" mass="14898" checksum="E7509626966DCF0A" modified="2023-11-08" version="1">MPPKAQKTPTTGGKAPAGKAPAEKKEAGKKTAAPSGDKKKRTKTRKETYSSYIYKVLKQVHPDTGISNRAMSILNSFVNDIFERVATEASKLAAYNKKSTISSREIQTSVRLILPGELAKHAVSEGTKAVTKYSSSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJH5</accession>
    <name>A0A9W9CJH5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-dependent RNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008267</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RdRP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.48" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJH5"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031380">
        <property type="term" value="C:nuclear RNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057503">
        <property type="entry name" value="PH_RdRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007855">
        <property type="entry name" value="RDRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057596">
        <property type="entry name" value="RDRP_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079">
        <property type="entry name" value="RNA-DEPENDENT RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079:SF17">
        <property type="entry name" value="RNA-DEPENDENT RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25358">
        <property type="entry name" value="PH_fung_RdRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05183">
        <property type="entry name" value="RdRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0696">RNA-directed RNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RdRP-like PH" evidence="3">
        <location>
            <begin position="159"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="RDRP core" evidence="2">
        <location>
            <begin position="485"/>
            <end position="1092"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1275" mass="145392" checksum="367D406C9C792F82" modified="2023-11-08" version="1">MDIFIQNVSHCHLGEKVEALSATKLRDDLNSMLTIPTQVPQNANHVELQLFLKDKLNQLDILAYDVYKFRAQNGRHFAILTVASVIQGNSFLQYYGSRGRQAPLKPLFFRGERLRFQRSNRPGQPEPLKIRTLQEKEETMRAKMKGQTPSAQIPRAHHSLPFQTLMTGVWDYNNQSKLFFDQKFKDMRQGYITFGSAALVIYLQEFAHRDFDWHCRIDIPYAILEHVLPSFGNGQRQGTITFTLKSPPKFYHIQSTEDLHLYAGKEAPPNDLPNDMLSALASLNLGPSKKAHRLERLCALSQSDTKNSALCMVYRIAFFDTRSAKYAWNYVKDFAIPEAHDWKTTESAAHLTNPIEVECYDVERILSDYRPSIPFEFDFQVRFQLMALMLEGTITPRKMIRMIPFVQKIARKHGSELTANGIRQLGRQIPTPAPNIDADQFSLDTLQASLFRGVQESQERRMIGQDLNSKHKKHQHLVLTYRSTVTPTGILLRGPDWGVSNRVLRKYSNHSEYFMRVFFADEDGLSVFHDPRASHEQVYARFRHVLQNGITVAGRTFDFLGFSHASLRNHQAWFLAPFEQEDGVIVCASDIIRDLGDFSHIHCSAKCAARIGQAFSDTIFSVTIPSTAYIIEVKNDVMRNGRCFSDGCGTISYSLLRQVWRALPPERRQQRPTVLQIRYRGAKGVLSLDSSLGGEQLHIRKSMTKYVAKESWKDLELCGAAYKPLSVYLNHQFIRILEDLGVPVSSFKSVQDEAVRTLKLITEHPLSAASFLQFSGSAVFAKIPTLFELMNQIGLSFHADQFLTDVVEVAAMAWLRSIKYRARIPIADGHLLYGIMDETNTLQEGEVYIPTQAFSDGKLDRSILVGTRIMVTRAPALHPGDVQLVTAVDVAEDSPLRSLYNCIVFSQQGARDLPSQLSGGDLDGDLFHVIYDRRLIPNITVPPADYASTPAKDLGRPVQVNDIVDFFVEYMNMDRLGQISNKHKIRADKHPSGTQHPECIVLAGLASDAVDFSKSGVPADMKKIPPGHDQIRPDFMAPGSNFVINKLGAAELEELEQDDADEPDVISILDADKFKPRYYRSDNVLGILYRDIDEKKFFDRMKHDFEMFRRTWGGESLVQKLSRYVDRETSGILWDHHQDFAEELREYYEDNMLEIMDTLRPHRGQPLTELEVFSGNILGKKERAPSRYIREANLEVQERFNRDVSGIIKRIIHGDGEWESARDAEALPRAIACFKVALEGKGWENYRTLKSWRYVTAAVCLEQLWQYQGRHLRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CJI5</accession>
    <name>A0A9W9CJI5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008223</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CJI5"/>
    <dbReference type="OrthoDB" id="3539644at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4365603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="432" mass="48704" checksum="F58D38F331BEEDDA" modified="2023-11-08" version="1">MVAAMLSCYSPTTTVFTPINSTPSTFAHRKLSEAKVEYDRCSLSIASVTCEFGYNITAEDTAAQQWHFLDSSMAKTRVRFRGEISVNRGSLFQVSDIEVRIVVTSNKENGLMNVHLQSSNSSLEINYFITDEGDIYTDAQILIFLRPWPKRLLDVLDIRSEVLDITFNDMLNWHVSNLFVHTSYGQSWYNSTYGADPLIVQNTTVSSYNGYIFGYYAAHGNLTFQNVHGQIGVILIPVNGRPSKLDSISASTLSATIHVEVEYGFTNWPTQPHTHVTNIHTRHGELYAFIPHGSYTNLSSTCSWLFARLQPFGNPNPEDLNEIYTYSELGWKTRVRIDDAILDGSEDLNNPLLNTISKHYLGEGSLWLRYPYSWFGSVEAQIDHGRLEFDATELEDFEKSEGYVKARRGKKGDSRLDIQVGTGAMDVHLGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKA8</accession>
    <name>A0A9W9CKA8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Replication factor C subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RFC3</name>
        <name evidence="10" type="ORF">N0V83_007452</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKA8"/>
    <dbReference type="OrthoDB" id="4199794at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000028">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000004">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000129">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF9">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="75"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="382"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="463"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="523"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="384"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="533"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4366922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="711" mass="79140" checksum="976774BDEDB63416" modified="2023-11-08" version="1">MSDIEDEMDIDAPVADSSITFGGDAKKGKRSAANLPVEAEDSLPWVEKYRPNSLDDVEGHKDIIATINKFVDSNRLPHLLLYGPPGTGKTSTVLALARRIYGNKNMRQMVLELNASDDRGIDVVREQIKTFSSTKQIFSAAPKAGDASLATFKLIILDEADAMTATAQMALRRIMEKYTANTRFCIIANYTHKLSPALLSRCTRFRFSPLKEQDIRNLVDKVIEEEKVNITPDATDSLVTLSKGDMRRALNVLQACHASSTPLQPPGKPTPDPSTIERDQITQTTIYDCIAAPHPSDIKYIIETLLSKNDMTECLRTINNLKIMKGLALADILTAVSEELVTNDVPPQVMVTWMSGLADIEYRLSGGGNEAIQTGAAIGVKRAPSNQQSLHDAANTEQPKQHARPIRNEPATDSNQPPVSGKVELRFSFTTASDPKTGGYSRDDPLNKSFSELLVATNTDERQLNHTRHTEPISYPTLPGTSSAPELTKPKKKREGYGFSYSETFWCDSDEYPDTECDWEAEDHPAQPCAHPPTSNKQGTDWNGQEQAAQASFGGSTVVNSGCSFDDAVVLRDVGSFHIARPQRTLPCTKATREDTIRLKNQAKALLAMYHESSNIQSQLLARLERMIYAEQRKDDASGRTRLHSVPYCAQHLQNFLLKIKEERVETGEHRRFVEHEIEWVKWIVEASRTGVMHLRATGCKCRPDWEEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKU0</accession>
    <name>A0A9W9CKU0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">VPS15_1</name>
        <name evidence="13" type="ORF">N0V83_006500</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="242910at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13980">
        <property type="entry name" value="STKc_Vps15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000497">
        <property type="entry name" value="Phosphoinositide 3-kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000342">
        <property type="entry name" value="Serine/threonine-protein kinase VPS15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045162">
        <property type="entry name" value="Vps15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583:SF0">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="25"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="9">
        <location>
            <begin position="438"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="1131"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="909"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1489"/>
            <end position="1521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="943"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="976"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1495"/>
            <end position="1509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1510"/>
            <end position="1521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4368144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1563" mass="175173" checksum="D2AB34DFCA790ECB" modified="2023-11-08" version="1">MGQGFSLTTLSAGSANIDVPELADLQYEKSLGAARFMKAVRARHKDGLVVARVVMKPYAQFDFQAYVKRLLHERKVLADIPNAVAYHRVIETAACGYLVRQYVHSSLYDRLSTRPFLEEIEKKWLSFQLLCAVRDCHARHIFHGDIKTENVLVTSWNWLYLADFSSSYKPAHLPEDNPADFSFYFDLSGRRTCYLAPERFLAAGQQPEGEGDVNWAMDIFSVGCVIAELFLEAPIFSLSQLFKYRQGEYNPEHSHLNKIQDGNIRDLIMHMIQVDPNSRMSADDYLTHWKDKVFPSYFYGFLQQYMYSITDPSSGRKPVTTGAEHLGEPDERIEQIYNDYDKISHLLSSSGGKDFSRFHHSPPNPNARLFPIFVNIPNYQHQASPTPSLAIDDGNLIFLTIVVSCLRGTARASARVRGLELLLAFSERLTDEAKLDRVVPYVSQLLTDKSEQVKIAALRTLTQILAMVQVVSPINAYIFPEYVLPRLEAYLPDSSTNVSPLIRMQYAFCIGTLAATAAKYLDMIQALRAEGSLPTADPETEDDLASSAHRNQFDSDRRILLEAFEKHTKALLTDSDASVRRAMLRSVSDLCVFFGSPRANDVVLSHLNTYLNDPDWMLKCAFFESIVGVAVFVGGASLEGYILPLMVQALTDPEEFVVEKVVRALSSMAELGLFQRSKIWELVDIVARLTMHPNLWIREAAAQFISAASRYLSVADTHSILVNLIRPYLKVVPSDFSEMRILESLKSPLTRLLIEMASNWALQAQKGLFWVPAKHQQTFTFGTSDDSLPAISGRELDHKVLQRLSKNEEDELWLKKLRNAGMSSEDDFKLVALREFIWRVSHRRRSDSLVSPLARFNSIVALKDLGVKALTVLFDEDVRNAVEHTRKSDTQQDGQTQPRTIRDALLDASTTEPDASTDRLPHAESQTGRLIREETRTRRLSRPARPSPSSSPSDIRSALDSPSSEAEARRSPLHIQKSSPTTQSPLGSLGTGDHVHALRHRSSALGLLSKGENKALAEIGTTSANAFGKVDGAHVRDSSLSRLPTSSLATDRRHTFLPRIRFRNAHNYTGNDPAVLKLLDSMLLEHFPSGEIEFGPRIQPAIRRQPIRYKDSHQSPPGVPWRPEGTLVASFGEHTAAVNRILVSPDQAFFISSSDDGSIKIWDTSRLERNITRRSRQTYRLGEDVKVTSLVFVEQTYSFVATGSDGSIHVVRVDYYQGQDGSAKFGRPRLLREYQLPKGDYAVWSEHYNEDNRSVFLLATNTSKVIALDLKIMEVLFTLQNPLNHGMPTCFCVDRRAHWLLLGTSHGVLDLWDLRFKLRLKAWACHGASPIHRLFQVFLPKVKRTRLYIAGGTGQGEVSVWDFEKHICKEVYRTGGCKDIGTKSTTLIDLDEEKSGGMLGRFATSLEPTASSTADRGIRALAVHTQAIDDKGESKHTFLLTAGPDWKIRYWDTNKVDSSMIVSGLEADETKPQYTSSQPAPDTIVVTERLSQPQSSASNGRDSRGSSSSKKTVQKSPRSGPISLQQQHLLKGHLDNILDVALIEQPYGMVISADRSGVINMFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKX5</accession>
    <name>A0A9W9CKX5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_006620</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKX5"/>
    <dbReference type="OrthoDB" id="10257314at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000003">
        <property type="entry name" value="Alpha-ketoglutarate-dependent taurine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051323">
        <property type="entry name" value="AtsK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468:SF31">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="8">
        <location>
            <begin position="92"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="363"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4368264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="399" mass="44755" checksum="B679255399FEED67" modified="2023-11-08" version="1">MAPGLVEPQYQPADAPTKASTLKPAIKSSNIHNYVPGRTTVQKHDETYEYEDLRPRFPDIKWPAYEEIPYVDKGQLGDPNFKNLLASATDVFDYNPKIGTEVHGVDLANLTDPQKNDLARLIATRGVVFFRNQKNFDIDEQRELGKYFGSLHKHATTGIPKREGLEDVHVIFTGEGSPDLRALFTPTFLWHSDVTYELQPPSYTSLKVLTGPPRGGGGDTLWSSQYAAYDMLSPHMQKYLESLTALHSAELQAQGSRDLGRTVRREPITTEHPLIRTNPVTGWKSIFFNPGFVTKIVGVPKTESDHVIGLLNEIVATSPEVHVRFQWGEGDVAFWDNRVTNHSASYGFAPHRRHAVRVAVQAERPYLDPNSRSQEDELSEKYGMPKANKDGSGIVNYND</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLK7</accession>
    <name>A0A9W9CLK7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLK7"/>
    <dbReference type="OrthoDB" id="3794824at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="10"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="550" mass="61477" checksum="4F128526E614C9C4" modified="2023-11-08" version="1">MASSSTPALRRSYDELPIELKREVCTYLPNSDLKTVRLLNDEWNHVASSPLFRVFTTNLVETKQRKLNTLLVSPARGGFVNSVKELNIRHQTVKTITQDVDSKLLRLLRVLPNDRLKKFSTNANVEESVISSLLQNQTQLRRLFLAYEFYREPPDSLPSIDLVQGSLQALESMTIFANGKHEDAYATWLPELAALRILKIEASQRSRRRTHNFTSWASPTVSSSLKLRTLTLENVRLPAELLHIVNAVHLPSLKNLFLFGCTNTEPLLDLMAQQLQASNSDTDTKPSKSKTFRYECLVKPSEDRLHHVQRFLSSITGLQAVLVDAVGSTPLAISSLESHSASLERLMLSFPTSRRSATDNLYTVRDLTQLVDTCPNLRILGISLANIDFEDWLPFTPVGVSLSPSNGPLGSIATTPEGLQIAQALVPLSRVSHLRTLVLTQVPYEQFQSDPAARIHRHTSLATEIFTFLAAHNSPVSYIVFHPVGYYETTPLSSPSSSIVTDASWPNFCYRRGKVVSDSAGPAFEGVVAIPMSREEFAEVELCHRLVMRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLY2</accession>
    <name>A0A9W9CLY2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLY2"/>
    <dbReference type="OrthoDB" id="5350396at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="593" mass="65297" checksum="E5B1AC6737C86903" modified="2023-11-08" version="1">MLTATIATSFDTAPPQPSQNSANSGASKRVVSNGEHVVLNSDSDSDSLPDLDWGEPKSSIKVPISTAPIKRASEDCEGDLRRPAKREKGGKRSFNRLVETAQRNMETERRIVERKADLDKSLEEPARAEATINEDVLGQVVPDDDDDPDKAHRLFLAMQRTNATQSESVFHFFDDSLHSENTQPKFPSNCLPKHRWASNFEDSATRDHAFLTGFAYQAHNEHLPKLLETNKLNAMFSSIGADMRSLDLDTKVAPSPVDLSASKIPLPASLKSITRLLRRAAPWLRSKDRSHALYILYHVCLDDRITADADVLSSIQDAIEAMICNFVDNHRLTAGVGCSPTQQNIQVLIFSQLSDAIPQLLSHITHPVLQRNLICALPASSPLTAYLQRHLALSFFLAPAVVDAPLADPKTPALVHKHLSTSPNFRINKDTDYGHLAARLTLLDIAIGPGFLSVPYQPLISPTLSPEDSSPVTAPYPVSSEVKDFNKVVDALATHIKLLGNSIVEGGAALDLTILDVKDCVERLCARLEHAVRIGGKKAHNVFGNEDDDEDKQLKMNKFFFKTRKTATSTAQHDSIFDKDVDRGVQLDDLLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM49</accession>
    <name>A0A9W9CM49_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ER membrane protein complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">oca3</name>
        <name evidence="6" type="ORF">N0V83_005017</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EMC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM49"/>
    <dbReference type="OrthoDB" id="124397at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001208">
        <property type="entry name" value="Tetratricopeptide repeat domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039856">
        <property type="entry name" value="EMC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055217">
        <property type="entry name" value="TPR_EMC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12760">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22890">
        <property type="entry name" value="TPR_EMC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="EMC2 TPR-like" evidence="5">
        <location>
            <begin position="113"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="256"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4370496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="330" mass="36486" checksum="6FE6346143D41E56" modified="2023-11-08" version="1">MPHANLLSPPSQIPPPTALALSQKAPLILASSPTSSLPWPLSLLFSRETPESWTIHENLFLSALRTGDETSARALLTRLEERFGEHNERIITLRAIYNEATAQSDSDLEKVFDGYEKILREDPTNMSVRKRRVAVLKALGRTQDAITAVTVLLQNSPTDVEAWAEASELYASQAAWGQAIYCAEEVLLITPNAWSAHAHIATLYYLSTASNTPPNLSALALSLKYFCRSIELNDAYLRGYYGLKLVTSKLTPLLSETSSSNNKRNNNSQEDDDVPIPTISTVKKLEEVATSKLAEIVRLYKAGTKGWTGFDEAEVIAARELLDRDGKVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN90</accession>
    <name>A0A9W9CN90_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_004514</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing (1-&gt;4)-linked alpha-D-glucose residues with release of alpha-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.20"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.20" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN90"/>
    <dbReference type="OrthoDB" id="5839090at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004558">
        <property type="term" value="F:alpha-1,4-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06602">
        <property type="entry name" value="GH31_MGAM_SI_GAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030458">
        <property type="entry name" value="Glyco_hydro_31_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF95">
        <property type="entry name" value="ALPHA_BETA-GLUCOSIDASE AGDC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00129">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040808168" description="alpha-glucosidase" evidence="8">
        <location>
            <begin position="19"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 N-terminal" evidence="10">
        <location>
            <begin position="115"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="9">
        <location>
            <begin position="289"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="11">
        <location>
            <begin position="713"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="463"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4371297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="918" mass="103475" checksum="DBE1909FA8523758" modified="2023-11-08" version="1">MLTQLLLALVACSSLSYAVPFPPSPYEVPPYPANVTSVPTPLSAGSACYGYIASNVVTTDSTLTADLTLAGAACNLYSEDIEDLKLVVEYQTNERLHVKIYDAALTAFQVQEQVLPRPKNENADASKAALKFDINEKPFSFSVTRQENDEVLFDTSEVPLIFEKQYVRLRTALPHHPNIYGLGEHSDSFRFQTKDYERVMLNSESPNIPVNSNLYGTHPVYFDHRGDKGTHGVFLLNSSPMNIDIKQDDDGDQWLEYNTIGGIIDLYFMAGKQPAEVSKQYADIVGYSAMYPYWTFGFHQCKYGYWDVNMVAEVVGNYSKAGIPLEVMWTDIDYMNLRQDFTTDPDRFPLAKMRELVSTLHERDQRYVLILDPGVHAVGNYSTYAKGHEKDVFLKGADGSDLLGVQWPGAVAWPDWFAPNTQQWWTDEFTTFFNPDTGINLDGIWVDMNEASNFCANQTCNPRQQAIDDGIPPVPTHSPRPNTGRPIPGFPADFQPTPSGSPSIKSRHSRSLSPRQSGGDMKGYPDRDWFYPEYHINSHRGNLTDYTIYTNTTNYDGTRQYDTHNFYGHMMAMTTHESMVTRRPALRPFVLTRSTFAGTGRKVAHWFGDNASTWEHYRTSIRQMLAFASMHQMPMVGSDVCGFNSNADPNMCARWALLGAFQPFYRNHAELSTIQQEFYQWPLVTEAAKKAIDTRYKLLDYIYTALYYQTTDGTPMINPLFFLYPEDDNTFGIQDQWFYGDSLLISPVTQDYSDIVTYYLPDDVFYNYWTHERVQGQGQNVTEEDVSYTDIPVHIRGGSIIPHRMNSANTTKAVRQEDFYLLVAPDADGNASGRLYLDDGESIEQGSVTEVQFSYKHGKFSATGEFAYAAASGESVTVGKVVVLGQDQGGAMGTYDAATHEVEVQGPWKLSAAWGFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CND9</accession>
    <name>A0A9W9CND9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminoglycoside phosphotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003766</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4371993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CND9"/>
    <dbReference type="OrthoDB" id="10003767at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051678">
        <property type="entry name" value="AGP_Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310:SF56">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="1">
        <location>
            <begin position="111"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="447" mass="50494" checksum="DBEC3BF463B27078" modified="2023-11-08" version="1">MERMDDFVHYAGNENTNDAASIASSDVSFTSTMNFEHEPFEVFQSKVAELCKEKWPRLSTDAFTITRMEGGSFNRIIALQVDTSKTQLPWIQRHARNFIKKVCSNPGRKSAIREYVLRIPRFEYAWVEHDIAVLLFLADKNIPVPKITSFTLSSNNAIGSPYTIQPRLPGQPKTGLDLTIPQRISLARDLGSALAEMSQLRSSCPGSLDPAGILNGSSHFQTLRFQCPLRNSFRPGSGEPFTPSAPQSVYDFLVSQIARQRAYDLTLHREATNPWKGLGKITDELNKMGLFHDDTYYLTHMDLMPRNILVNVTSPTTARLSGIMDWDEAVFAPAFLNCCPPSWLWDFEGDEDLDEKVANETPADPEVQAVKKAFEDAVGSQYLTYAYSTEYRLARTICLLAITGLHSNDETYMARDVIDEWNEMKPECQVPGIWDFDSDDELDENDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNG6</accession>
    <name>A0A9W9CNG6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tryptophan--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Tryptophanyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">WRS1</name>
        <name evidence="14" type="ORF">N0V83_003400</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNG6"/>
    <dbReference type="OrthoDB" id="10261385at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000003">
        <property type="entry name" value="Tryptophan--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000033">
        <property type="entry name" value="tryptophan--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055:SF1">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055">
        <property type="entry name" value="TRYPTOPHANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 12 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="432"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4373109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="493" mass="56132" checksum="F04312A81FC0EEE2" modified="2023-11-08" version="1">MTEPEPPSVAALSLAETATQPVSKATEQEINPWDVQAAVDEDGNVKEFDYAAISQQWATKLVDDALLERFERVTGHKPHRWLRRRLFFSHRDLELILDTYERGEDFLLYTGRGPSSDAMHIGHTIPFEFTKWLQDVFDVPLVIMLTDDEKFLFKEKLKQHEVYEFSRQNAKDIISMGFDVEKTFIYIDSEFFTSGYNRHFSLNATEFEKLITNNQVRGAFGFHGSTNIGSNAFAAKQCVAAFASSYPFIWGQDIKTYHRSQKLAAMPCLIPCAIDQDPYFRLVRDNCSRMDHPSPKPALLHSKFLTALQGAGGKMSASNPNSAIFMSDTEKQIKKKINNHAFSGGQETLELHREKGGNPDIDVPYQYLSYFEDDDAKLLRLADEYRKGELLSGEMKKECVDMMSQYVKKFQDARSKVTDEVLEEYLRPRKLEWKGNPNPTKPKVEKTEETTQGGPTLDADGKPMTKNAMKKALKMAEVEKKKKEKEAAAAAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNP4</accession>
    <name>A0A9W9CNP4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WD40 repeat-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004052</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNP4"/>
    <dbReference type="OrthoDB" id="20669at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991:SF12">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="473"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="17"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="651" mass="71753" checksum="D8E2FA17C0B35A7A" modified="2023-11-08" version="1">MANPHLPSADDDYWTAPHTMTTASTSQTHTPTATATATATASSPPSPTAIAAAVTLATLSDSRPPTSHGEDADEDHDHEHSGGVLLADWQFYVHDATLPSTYIMEEEEDVLALPQSVMAHFPTGLQLTPSSALDMFDPSDPYYMPSAHAWPPVSMSHMLSSEHLLEPPPTLQETQLHIQDKGSFFPLTSFFHDVVRLKDKERAERIVPGLELIEPPKSITREDLQGDSYDCQGIDWSVRNTTRLHVRAKRVEVETEKLFRTQKEVRKCIPSVPNTNNFFQFRRNNTTHRPFCPHFQLRNVIAASSRNDVFYASGHRVLRTDAEGSPADVVTDLSKRLAPDGCSITTIASAGDVLIAGAFEGEYAITDLSSTYGSSCTFGRTADYAADTKSRIVNHMHLSNSRTTYTPQAVLCSNDHRLRILDCATNTFTHSFLYPAAVNCSATSPNGRMRVVVGDFQETLITNAETGQPFETLNSHTDDAFACAWADDGIHVATAAQDSTIVVWDARHWGKPLTVIRSELSVPRSLRFSPIGSGPRVLVAAEADDFVSIINAQTFESKQLFDYFGPIGGTAFTPDGQSLFIANGERRFGGIIELNRTGWAEKTARTKSWDDVGDEVMTDWVHDYAMNDHERVVGGDMGRQRRPVDLNQLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPE7</accession>
    <name>A0A9W9CPE7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003569</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPE7"/>
    <dbReference type="OrthoDB" id="4194555at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="251"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1089"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1113" mass="123923" checksum="267E6C6E514497E4" modified="2023-11-08" version="1">MDLQNTLQTTASSQQTLWERRGSLGSGDIPREASYEEDLANALEDELLENEALQASTEQDPLSPSRVAPWTSTTAGAHKETSTSRHSLSNSDNVYDDWDDHAFDIDARLARIIRHTGGLHSSVDDTAIGSSSLQSLRLMIMASTKRSRDKYERAHAKDIATKLDEIGAVAIVLFESTTPNPKANDIWPEFRRQTKLPARYLLLDRNKEPPMSLSMSRRSRKQSRNAAELQPDLDRENKRHELVTAGRPHWNLPVELVELIASFLNRDDIKSLRLVSRELNFHVSQVIFKTVVVPFNTEIYGMLGQEPKPDFKGKKRARIERVGYLWKNANGDEVYNGHGLDVFRGFGKHILRYGMSFEVNEESLATPPAKSLTEKKTSFWGDYDWPFEEYRRFDTVAGLETAADETPRMKIAFSELSKVRELALSVDSGLGWLNGPDRSIRARILQRPPQVFGTLKAIPDRRAQAQQELWRVIKECHELHDKDIRLATLYKMDGRNGQRFLSELKEADMAAEEQPNMPYLDPRLIHEATPHDFGGLPIPASFDDPTVLDDFVLRPPSDANATGVLFSSNKKPPDAGEVMSSIIPANLTKAQKEWLLETEWAQRAFMSSYMLSIIDNPVTFKLVHTLTIAQLSARYLPMLHRADFWTALPNLTNVTLLVIPEWRTVHKDDAGFVDTPKTSPSHAIPPFYNLLQSHVACRPSIRNLTVGWVTGGEHAEGLHARNKLLLPAPLMGLGASEMLTENDVNGLRANLLHFVHVKRLTLKNCWITPPALVQFVNIHDHIFKHLVFDSVSLTAILRPNANVNQAAQPAAGLNPPIAGLFGPGFINLLHHHGGAQAGVPTAQLPPGQQVLQIYMQALHIQLQQLQANAVGTQQHNLVIALQNQLQQVQPLQNQNPQQPHVHAPGQPHVINQNFLGNLVAQVNQLQQLIIAVQAPLGPANIQPLADPQSILKAPPREGSWMNIIDIISPGTNLSDFGSKHSQAESQRSTSIQSIEFISCGYARLPHAPFDQTAIGPYHGIPPNFRKEAFTKRYSALCPAMLSASSAHLGEIVQEFDVSEFAALAAGWDMKFGWDDEEEARAVEFDGLRPGGTGRFSGKVQASDRMDQDSSAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPK1</accession>
    <name>A0A9W9CPK1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003586</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPK1"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4373292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="400" mass="45550" checksum="1B632DA567E1CDBC" modified="2023-11-08" version="1">MVMTFPKLATALRKLFTSKTASADTVPQKQFERVLPQPAARPPNMQIGQSRFQEHFDTSLTEIPTSWLEGHNPKLSKINKWKHRLCVNQKVLSFFEDFLTQKVLEPARQSKVQTNVKPLLPLTSDHVERDIHVLTTPKQSYKGRFRDSRLIYPGSSLRHQDNFVDIEKRAGHVDLAHVLSGLVDDYFASNNGPQLPKLPSPALLRCDSFVECDATVHCKGKISSRVHHPEQDRSVDGLYRADSPDVQPDDVVAASYIDDERLHTEETFEYPCLEDAVERCQSSSLPMVGVQQQRVEVLFSNAVQAPAEPDMQVVPTTTVAGVCEWKTENVPQVARMFGWESFVLDEEPETEAEWVVDGSKEMPTVESLRDHFEEVNWKEMHLVSADAFLEWYQFRTGQSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPM4</accession>
    <name>A0A9W9CPM4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Malate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_003662</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPM4"/>
    <dbReference type="OrthoDB" id="1859733at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021851">
        <property type="entry name" value="DUF3455"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35567:SF1">
        <property type="entry name" value="CONSERVED FUNGAL PROTEIN (AFU_ORTHOLOGUE AFUA_1G14230)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35567">
        <property type="entry name" value="MALATE DEHYDROGENASE (AFU_ORTHOLOGUE AFUA_2G13800)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11937">
        <property type="entry name" value="DUF3455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4373367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="19330" checksum="CA4D98C35FE0E519" modified="2023-11-08" version="1">MDSPTPLAAVSAGLTLRHIAIGRGTQNYTCPSTASSDVPTAVGAKATLFNATCDAARLNVKVLTDVTKLALNYAIPTAPEAVKRISGHHEFTDKGIPLFNLKTNLTDFGYVQAKPDAVKSAAPTDASSGKNGLGSVPWLKLNAIEGDYKEVFRVETAGGVAPKTCDGITGGFSVDYSAQYWFYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQB1</accession>
    <name>A0A9W9CQB1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_001921</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQB1"/>
    <dbReference type="OrthoDB" id="203908at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051603">
        <property type="entry name" value="Zinc-ADH_QOR/CCCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44154">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44154:SF1">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="2">
        <location>
            <begin position="11"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4374843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="329" mass="34907" checksum="BDCE3CFB59EA9B15" modified="2023-11-08" version="1">MMKAIVLRQPGGPEALQLENLPIPQPQAGEVLICVKAFGLNRSELFTRQGHSPSVLLPRVLGIEAVGVVKSCPGGELHEGDVVATAMGGLGRDRDGGYAEYTCVSCENVQTISNNVAEELGWETLGAIPEMLQTAWGSLIKGLKLGKGDKLLIRGGTTSVGLAAAAIARNYGATVAGTTRKTDKHTAELMHQNGITHVIQDSGTIVGSVRETWPGGADKVLELVGTKTLDDSQLCARPGGTVCMAGIVGDKWTLDNWNPMESIPTGTCLTSYSGGRDDFIQTPLSDLAEDIRKGNLRLQIGKVFKLEEIVEAHRAMEENRAGGKIVILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQI4</accession>
    <name>A0A9W9CQI4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">DNA replication regulator SLD2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RPN12_2</name>
        <name evidence="10" type="ORF">N0V83_002132</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Has a role in the initiation of DNA replication. Required at S-phase checkpoint.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the SLD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQI4"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902977">
        <property type="term" value="P:mitotic DNA replication preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22289">
        <property type="entry name" value="RecQL4_SLD2_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1460:FF:000001">
        <property type="entry name" value="DNA replication regulator Sld2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021110">
        <property type="entry name" value="DNA_rep_checkpnt_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040203">
        <property type="entry name" value="Sld2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28124">
        <property type="entry name" value="DNA REPLICATION REGULATOR SLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28124:SF1">
        <property type="entry name" value="DNA REPLICATION REGULATOR SLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11719">
        <property type="entry name" value="Drc1-Sld2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="57"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="163"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="283"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="175"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="336"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="411"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4375052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="458" mass="50797" checksum="2C582C75D7CFCAA7" modified="2023-11-08" version="1">MSTTDIEQRCTVLRHDLKAWEKEFAAANNGQRAGRDDIKANAEISQKYKEYNKLRVRLSSKAAPQTPSKRAASRKPTLDVERTPKGPPKALMSTPMKRKRDEELSTENAGLAHEVLSPQGPTVIGPTPQRDGIVLGLFDLLPAETPSKKRAILGDVEPNVLQTPSRKFQEAASDTSRESRARGERTPLSSGKRFLFNQFVTPKKRKLDEEGTPTSTARGLATPAFLRRDNVLSVIDEADETTPRPAPWKRRGLGRSLSAMIQAMKKDEDDRLDEEADIMRELEMEEEGIPVPKKPKVPQILVEDSQMAMPLGPDRGFESEEDSEEEPTLGRDGKPRKAWKKKGLKRQTRRVIMRPNFAKPKPQPTPQVQDDSEAEEKGVAETQVQANNAVDDRGFDDDGSDYASDASHAPKKTEAPKKEGVVKTAARKIKATANANYRRLKIKGTGGNGGKGKSGRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQP1</accession>
    <name>A0A9W9CQP1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mannosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_002318</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyltransferase 22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQP1"/>
    <dbReference type="OrthoDB" id="497541at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF2">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE ALG9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040718449" description="Mannosyltransferase" evidence="11">
        <location>
            <begin position="23"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="333"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4375232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="572" mass="64303" checksum="56D3BE69D72962F0" modified="2023-11-08" version="1">MAFYVFALANLIAAGFSPIQDCDEVFNYWEPSHYLNHGYGLQTWEYSPEYAIRSWTYTGIHSLFIWISTFPLKALGYGNSKVAEFYFLRTLLALLCALCQTRLFSVISRTFNPRVALFFMIAMVISPGMYHAAPAYLPSSFAMYTTMLGFSAFMDWSHGIKTSQGVMWFGLGSILGWPFAGALVIPFVVEEILLAAITGEVIGAFWRLLQGAGRSLIILALQTAIDSAFYKKFTCVPFNIVLYNVFSGGSKGPDIYGVEPWHFYIRNLALNFNIWFFLALAALPLLLVQHIGFQKAVSKQTLIRSIVFASPFYLWVAIFTAQPHKEERFMYPAYPALALNAAISLHILLANFGSTDPRHLVSKIPPQLKLAIVSIPLLLSFNIGVLRTFGTLTAYSAPLNVYKPLQNAGAARPGDTVCLSKEWYRFPSSYKLPHQVRAKFVKSEFSGLLPGEFSEANIGFGHFPGTWLVPIGMNDENQEDPGKYIDVDQCNFLVDSRLPGTPPTVLEPDYISDTEHWEILKCVPFLDASRTHLIGRLLWIPDFPFIPTRFRRKWGDYCLLQRKASGSKGLHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR46</accession>
    <name>A0A9W9CR46_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Membrane insertase YidC/Oxa/ALB C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_000427</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR46"/>
    <dbReference type="OrthoDB" id="2148490at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20069">
        <property type="entry name" value="5TM_Oxa1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="YidC/Oxa/ALB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF66">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEIN OXA1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="289"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane insertase YidC/Oxa/ALB C-terminal" evidence="12">
        <location>
            <begin position="131"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="458"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="469"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4377600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="495" mass="54987" checksum="37508DAD9B6E841A" modified="2023-11-08" version="1">MDHVPIADFSNHTVAQSPAQPASEFANAHQPVVTTATPELAPAGIDKAAVVDGTSTSATAESASPFTDSKTVEELLGLQVEPTPAKVEFDPTQLVDHPGQLQELGLEYGWGMTTLFEKTIEQIYLTSGWGWAGSIVAAGVVVRCATFFFQALSSDKMATMAALRPVTQPIQEKMEAALARGDKGQADIYKMQQQEILKPYMGGFVSMGGFMMVQMWMGFSAFRFLRAMGELPVPGMSTDGFLWFTDLTVRDPYFILPAMTSAIMYTVFKTGGETGVQQEVGQAAQRQKMFTGLAVFLGIVTAFQASGLQLYFLMSGIMGGITGWLLRQNGFRRMINIRTIPSKESNEIYTRVAKGELKLNDIKTRDGKIRYQAPTPAPKPTNRRNATTLSGINIKAGTAIPAHLRPEAPVVDTDRPDRDMDFEEGAQGKPLSQKLDYYRRNYRLSYMFRRLKGGIEDMGRRAGYGGAKTSKEQERRKRRAEEYEIERRRRFENRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRH9</accession>
    <name>A0A9W9CRH9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_001718</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRH9"/>
    <dbReference type="OrthoDB" id="10030083at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03390">
        <property type="entry name" value="PAP2_containing_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000035">
        <property type="entry name" value="Putative Lipid phosphate phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF84">
        <property type="entry name" value="PHOSPHATIDIC ACID PHOSPHATASE BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="8">
        <location>
            <begin position="58"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="278"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4376434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="333" mass="36539" checksum="D177457681DFF0B4" modified="2023-11-08" version="1">MVIFQDGEIVFPQFAYPMRNEIVPIWLAALLAFIIPFIVFLIIQIRARSFWDVNNATIGLLYALISAAVFQVFIKWLIGGLRPHFLAVCKPVIPAGLGVGANDDGVEGGINGNVANGYRQIMFDRSVCTGDRKEINDSLESMPSGHTTAAFAGFVFLYLYLNAKLKVFANYHPAMWKLIVIYAPLLGACLIGGALTIDEYHNWYDILAGAFIGTMMAFSAYRMVYASVWDFRFNHIPLLRHSPFVYGAGPSSFDGFHDTLFTRKAGWGTHEGASWGGAPFDAADGPRGTMAPHPDGPVHGHGHGHHHDGSMGSTTGRVQRKPIVGDRHGEQMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XX72</accession>
    <name>A0A9W8XX72_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010779</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XX72"/>
    <dbReference type="OrthoDB" id="408373at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050228">
        <property type="entry name" value="Carboxylesterase_BioH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43194">
        <property type="entry name" value="HYDROLASE ALPHA/BETA FOLD FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43194:SF2">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN LPX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="185"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="447" mass="49816" checksum="3720B61779057C5D" modified="2023-11-08" version="1">MSGWADLPAALEGSLDELTKLLQSDGQLKAFTTSNAIDKAATFGIKSSGSDNTLLITVTNGNAEASTGTSKDALFTLSALPEQWEQHFKQVPAMPYQSYWGMFGMNIKQKGIEVLGDQTAFAHWTHVWRRVLELVHDAKCGPMKEDEQQEPENDYLTGRYTFLEAPVWGRCKVFYEYSGEGKQPIVFLHTAGSDSRQYHGVMNDARMRKRCTMYAFDLPGHGRSFPSKSYYPGAHTNTEDSYVGIIAVFVKSLGLRRPIICGASMAGQVCLAVAIRHKEVGAGGTIPLQGSEYLNMERQWHDRSPYVNQSLFNPEWIYGMKSPTAPLANKQLIWHLYSAQAYGIFHGDLDFYFGGWDGRSRVASIDTQSCPVYMLTGEYDWSNTPEMSQKTADKIPGAMHKAMPDLGHFPATENPAKFVPHLIEAIDHIQKVRSENLSTMRLGNGTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY25</accession>
    <name>A0A9W8XY25_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GTP-binding protein rbg1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RBG1</name>
        <name evidence="5" type="ORF">N0V83_010962</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY25"/>
    <dbReference type="OrthoDB" id="603at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000003">
        <property type="entry name" value="Developmentally-regulated GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000740">
        <property type="entry name" value="Putative GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1070">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="3">
        <location>
            <begin position="65"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="4">
        <location>
            <begin position="291"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4362021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="399" mass="44114" checksum="3AF963C71D069EE0" modified="2023-11-08" version="1">MATTVDKIKDIEAEMAKTQKNKATSFHLGQLKAKLAKLKRELLEPSSSGGGGAGVGFDVARTGVASVGFIGFPSVGKSTLMSKLTGQHSEAAAYEFTTLTTVPGQVMYNGAKIQILDLPGIIQGAKDGKGRGRQVIAVAKTCHLIFIVLDVNKPLTDKRVIENELEGFGIRINKRPPNIVLKKKDKGGINITSTVPLSHIDHDEIKAVLNEYRMANADVAIRCDATVDDLIDIIEAKGRSYIPVIYALNKIDAITIEELDLLYRIPNACPISSEHGWNIDELLEQMWEKLNLVRVYTKPKGRMPDYSAPVVLRSTACTIEDFCNSIHKTIVEQFKHAIVYGKSVKHQPQRVGLAHELADEDIGTYFPVQSESCDFRYYIKGFWPCDRESQQKDDEQGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY59</accession>
    <name>A0A9W8XY59_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">60S ribosomal protein L16A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RPL16A</name>
        <name evidence="5" type="ORF">N0V83_010912</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY59"/>
    <dbReference type="OrthoDB" id="1882297at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3250:FF:000001">
        <property type="entry name" value="60S ribosomal protein L13a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1180.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005755">
        <property type="entry name" value="Ribosomal_uL13_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_uL13_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01077">
        <property type="entry name" value="L13_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4361971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="202" mass="23057" checksum="73F3B089074BBCD9" modified="2023-11-08" version="1">MSTFEPVIVIDGKGHLLGRLASTVAKQLLNGQKIVVVRCEALNISGEFFRAKLKYSAFLRKQTRFNPTRGGPFHFRAPSKMFWRTVRGMIPHKTARGKAAMERLKTFEGVPAPYDKKKRVVVPQALRVLRLKPGRKYCTVGRLGHEFGWKYQDVVARLEERRKVKGAAYYERKKAARRQLAEAKKTAKVDGKTQEHLTSLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ11</accession>
    <name>A0A9W8XZ11_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleolar complex-associated protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_009793</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for synthesis of 60S ribosomal subunits and the transport of pre-ribosomes from the nucleoplasm to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
            <location evidence="1 5">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ11"/>
    <dbReference type="OrthoDB" id="10263597at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011501">
        <property type="entry name" value="Noc3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016903">
        <property type="entry name" value="Nucleolar_cplx-assoc_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14428">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14428:SF5">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07540">
        <property type="entry name" value="NOC3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028977">
        <property type="entry name" value="Nucleolar_complex_p3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Nucleolar complex-associated protein 3 N-terminal" evidence="9">
        <location>
            <begin position="119"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="CCAAT-binding factor" evidence="8">
        <location>
            <begin position="478"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="450"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="360"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="13"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="453"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR028977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4363498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="679" mass="76137" checksum="6DC57BAD4F7954F8" modified="2023-11-08" version="1">MSQVPSAKRRRLSPPEHGERTNGATQDHKETFMKHAAKWDLEQDYETRPRKLKKQKETKKLPVRTTEGWVAHPAAAEDVKSDESESFLGSGSEADEELNEEEQVEDQKPKIPARQQIFEAKEELARIASLVNEDPEEHIGALKTLAAIADSENITVKKLALATQVAIFKDLIPGYRIRPLSEEKMQEKISKEVKKLRMFEQKLVSSYEAYVKHLAKLAKSSGSDKEQIASLASVAVSCTCNLITAVPHFNFRGELLKILIGKLSTRRVDADFVRCREALESLFENDEDGNVSLEAVTMLTKMMKGRNYHFDESVLNTFLHLRLLSEFAHKASYNSVDKSEDQPLSGKKMKQKREFRTKRLRKELKEKKAIEKEFKEADAAVSHEERDRMQAETLKMVFVAYFRILKARSPKLMGAVLEGLARYAHLINQDFFGDILEALRDIITTAEVSAAAEAEEDSDAEDSDSDDEAPDRNLTRESLLCVITAFALLEGQDASKAASTLKLDLNFFITHLYRTLHPVSLNPDIELSSKSLHLPGPDAAQSHAETAKINVQTTIVLLIRSLSSVLLPATSIRAVPPLRVAAFTKQLITIALHLPEKSCTAMLGLLNRITKTHGKKVAPLWNTEERRGDGVFEALKPEIEGSNPFAGTVWEGEILRRHYCPTVREAVGGIEKNILEAIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZE3</accession>
    <name>A0A9W8XZE3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_010210</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZE3"/>
    <dbReference type="OrthoDB" id="10656076at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4363090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="256" mass="29330" checksum="C05EA1E5353D49E3" modified="2023-11-08" version="1">MHEFHKNIVEIKNQFMAVRKILEEFKDIDGKVIKKLEAIEKEGENLKISFLAKLIDECYKKGYPRLPTHIEIQPDLWDAACDLLKESRQAVPVYTPQTKDGLGYPEGPQVGLQGQLRTRTEPPIPDEELGAARIRSPNCINRYNTVGIEDGLGERDVWDSKNSRSEQEMPSPKYQVVLHVYNIDKTKLTKTTRPTIYTEVDVANKVALEWLKDFFCEDEVVRVRDKELGMKEYAAQNVKNNQVAAWAEVVKVADEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0F8</accession>
    <name>A0A9W8Y0F8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_010287</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paxM FAD-dependent monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0F8"/>
    <dbReference type="OrthoDB" id="16820at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000270">
        <property type="entry name" value="FAD/NAD(P)-binding domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050493">
        <property type="entry name" value="FAD-dep_Monooxygenase_BioMet"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13789:SF315">
        <property type="entry name" value="FAD-DEPENDENT MONOOXYGENASE MDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13789">
        <property type="entry name" value="MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="7">
        <location>
            <begin position="15"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="8">
        <location>
            <begin position="125"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4363167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="486" mass="54626" checksum="8BD9F8761CFABE98" modified="2023-11-08" version="1">MEAKPLGSYPPSGIDVLIVGTGLAGLVAALECTRKGHNVRVLERNADINTAGDMYFMGLSATRFLKHWPELLKEYKKISLHNAWIETFKHSGEVVIGPKKVADRLRAQGLDPDTPPGEFQMRPLVYKMFVSAVENLGVSVEFNRKVVDYFEDEQTGKGGCVTDDGQRYEADVVIAADGVGSKSQKLVGGQVRARPSGRAMWRAAFPREELDKNPKVKEFFKMMPGNEPIVRTWLGPSTYALTLSREDVMVWIMNHDVTGTEKESWNNTIEKEEVLQGMEEMPGMEKVKWAPIFKELVEVTPPNTIVNFELLWRNPQPSWTSPGARIIQIGDAAHSYLPASGNGATQAIEDAISIASCLQIGGKENIPQSVRVHIRFRFIRNSCAQKLGFSNAELLQDTDWDKVKLDPRRAAPKLPKWVWSHDPEAYAYENYEKSAASLKKGVHMRDEDAFEPNYPRGYKYEPWSIEKIMEDMRAGKPIELGAGDWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0H0</accession>
    <name>A0A9W8Y0H0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein phosphatase 2A structural subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">TPD3</name>
        <name evidence="6" type="ORF">N0V83_011021</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphatase 2A regulatory subunit A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0H0"/>
    <dbReference type="OrthoDB" id="340346at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000011">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit A alpha isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054573">
        <property type="entry name" value="PP2A/SF3B1-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051023">
        <property type="entry name" value="PP2A_Regulatory_Subunit_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648:SF4">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2 (FORMERLY 2A), REGULATORY SUBUNIT A, BETA ISOFORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A 65 KDA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22646">
        <property type="entry name" value="PPP2R1A-like_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="88"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="204"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="245"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat" evidence="4">
        <location>
            <begin position="277"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="284"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="323"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="362"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase 2A Regulatory Subunit A helical" evidence="5">
        <location>
            <begin position="369"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="401"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="518"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="581"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4362079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="613" mass="68389" checksum="CDF0C7AB1C1E319B" modified="2023-11-08" version="1">MEANQNDELYPIAVLIDELKHDDVLLRLNAIHRLNTIALALGAERTREELIPFLDESVEDEDEVLTALSEELGKFVEYVGGPEHAHVLLSPLENLAAIEEPLVREKAVESLNKICEELSQQQIEDAFIPLVLRLSKADWFTSKISATGLYQTPYTRATPPSQEALRQHYGQLVHDDTPMVRRQAANNLAKFIKAMPPAIVIEEMIPLFQHLAADDQDSVRLLTVDILIAIAEAVPKEQQSSHGVLLTALRSLFEDKSWRVRYMVADRFEKIAKAVDEEVVNRDLVPAFVKLLKDTEAEVRSAIAGQIPGYCALLERETLLHDVMPSIEELVSDQSQHVRAALGTQISGLAPILGKEETISHLLPMFLQMLKDEFPDVRLNIISKLESVNNVIGIELLSQSLLPAIVQLAEDKQWRVRLAIIEYVPLLASQLGVKFFDEKLSSLCMSWLGDTVFSIREASTQNLKKLTEVFGVEWANDAIVPKVMAMGQHPNYLYRMTTCFAVSTLAPALSLDVIEQSILPMMDKLVNDDIPNIRFNVAKSYGQLIATLKQLPEEGTVIALDKAGTPGQGCQKATDLITKSILPNLEKLQQDDDVDVRYFATTAAQSFTEAMQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0V3</accession>
    <name>A0A9W8Y0V3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0V3"/>
    <dbReference type="OrthoDB" id="4158258at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="194" mass="21577" checksum="A030ADDFBA0470ED" modified="2023-11-08" version="1">MIENKPIGILISPRQPYHQTTPNEPKPSTMDKETEAKNNRQTEATQEDQAPPTYTPTSSSSSPPTYSSLSDSVRSSMDSLLHGDPTETISVPMITRRETTSIHNYNPFSRKSGKVVQSVHVRRMTREQYLKHYAKDAEGKYVGSGTAAPDAALVFVPEKSTEEDLLRQVEEVVRGKQEVRGKGIGKFGMPLDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0V7</accession>
    <name>A0A9W8Y0V7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mitochondrial inner membrane protease ATP23</fullName>
            <ecNumber evidence="9">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ATP23</name>
        <name evidence="10" type="ORF">N0V83_010152</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Has a dual role in the assembly of mitochondrial ATPase. Acts as a protease that removes N-terminal residues of mitochondrial ATPase CF(0) subunit 6 at the intermembrane space side. Also involved in the correct assembly of the membrane-embedded ATPase CF(0) particle, probably mediating association of subunit 6 with the subunit 9 ring.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Peripheral membrane protein</topology>
            <orientation evidence="1 9">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the peptidase M76 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0V7"/>
    <dbReference type="OrthoDB" id="285308at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019165">
        <property type="entry name" value="Peptidase_M76_ATP23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21711">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21711:SF0">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE ATP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09768">
        <property type="entry name" value="Peptidase_M76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4 9 10" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4363034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="241" mass="27881" checksum="4A9E34EBBB7432B8" modified="2023-11-08" version="1">MSTPEQAPDGASIEAIHQPPPEKIDPIYYNWSSTFSILLGRMTGNRDVPLERSYFTEMDALKSDSICKKCETNKEYLLQYSPIVRFLNDEVSKLGGDLNKDNILCRMCTNEQSGGFSLNHGILLCANKFRNRGHQEDTMAHEMVHAWDHLKWKVEDDNLRHQACLEIRASTLSGECRFSREFFTRNQWTITEQLQACVRRRATLSMLARPGIKDQEHAGKVVNEVWDSCFKDTRPFDEIYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y172</accession>
    <name>A0A9W8Y172_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carboxylesterase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008843</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y172"/>
    <dbReference type="OrthoDB" id="3946796at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="505"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="526"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="555"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="570"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="586"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="605"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="660" mass="71377" checksum="0388F803FF2351C1" modified="2023-11-08" version="1">MRLTRTRAAQRAQEGNNETTETSDLDERAPLNEISPNASPDLGKHKEELPKKAPAKKTKGKGGAKKGVKGKKGKTTEEEEQEAAEVPASDTAVAVDSPVADPSIVVDHILPSNETVVAPSEPVRLTRRQIAKQEEEELRQSQRTGSPLGDAEPAMESIEEERGAESQAVESREPQEELAGAPKTPQDPVLSTESEATATEAEHVETEGTNPSAVEAVAPSVEITSEPEPKTLATERPSDDAVTPVASRTPSRRASRSPSKSPMRIEESIEALDALEEALENVEAVTSFNHSSDEVSPRKKAVTPKVGGKTPSKTPLAASRISRTPSAAPKSVKPTKTSLARASSVRTAPSKDVRKGSTETVDYLATKRRPISMSFPTPPPPPKGRAPTRSTFQLSSDAVAQKLKAEKEERLKRQAEGGPKPRPISMPPPPKSTKPPTKASFQLPGEKIAAELKAKKEERLKREAEAPQQPGAPRAPRPISMPPPPKSTKPPTKSDFQLPGAAVAEKLKAQKEERLKRQEEAEAAKKAAAANLRQAPVRKPVTVPIRQQPGISIPSPAQRSSSIASKRDSIQLSRSTSTSSANRQSMLLNAKSTVTPVDAAQQKLKGREVFNRDKMEKEARERERKEKEDAAKRARAEAAERGRIASREWAEKQRKKMMGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y176</accession>
    <name>A0A9W8Y176_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_009975</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y176"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31794">
        <property type="entry name" value="AUXIN EFFLUX TRANSPORTER FAMILY PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31794:SF4">
        <property type="entry name" value="AUXIN EFFLUX TRANSPORTER FAMILY PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="157"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="164"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4363679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="342" mass="37783" checksum="F273B45FB9316F04" modified="2023-11-08" version="1">MDVDASELIVPFVGAIQASFSVLLTIAFGVVAAQCNLLSVRAAKEVSKLCVRMFLPALLIYKIGSNLHKDTGVRYIPVLSRALTRIFKLPAWVTPAIAFNNTTSLPLLLIQSLQQTQILDAILMDGESGSDAMDRAESYFLINAMVSNSLTFALGPRLLKPGDEDAPDDKEDREVEEEEQDEGEEDGENGDIERGPDGVINEHTSLLPQRIVHPTNRVEKKSYLKTRNWYNDLPPWAQETLDVAWQFANAPILGAIFGAIIGLVPALHRLFFNQSNEGGYVNAWLTTSIKNVGNLFASTQIIVVGVKLSQSMLRMKRGEDSGEVHKGSLALVTFFRFILWPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1C6</accession>
    <name>A0A9W8Y1C6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Guanine nucleotide-binding protein alpha-2 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GPA2_2</name>
        <name evidence="12" type="ORF">N0V83_009160</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the G-alpha family. G(q) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1C6"/>
    <dbReference type="OrthoDB" id="5817230at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010255">
        <property type="term" value="P:glucose mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000007">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000181">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000692">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002975">
        <property type="entry name" value="Fungi_Gprotein_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF369">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-2 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01241">
        <property type="entry name" value="GPROTEINAFNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="45"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="153"/>
            <end position="154"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="178"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="203"/>
            <end position="207"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="272"/>
            <end position="275"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4364564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="355" mass="40866" checksum="F47CE52AF4174E1A" modified="2023-11-08" version="1">MGICMSTSNDDVEQKKRSQAIDRKLEEDSRRLRRECKILLLGSGESGKSTIVKQMKIIHQNGYTQEELAMYRLTIYKNVIDCAKALIGAMRQFEITTENPGNDEYCSYLLDYTVDPDPEKPLDSRVGQAITSMWRDPCVSKVLEHSSEFYLMDSAPYFFDEVERIADPNYIPIESDVLRARTKTTGIYETRFTMGQLSIHMFDVGGQRSERKKWIHCFENVTSIIFCVALSEYDQVLLEESSQNRMMESLVLFDSVVNSRWFMRTSIILFLNKVDLFKAKLARSPLGNYFPDYSGGNDVNRAAKYLLWRFNQVNRAHLNLYPHLTQATDTSNIRLVFAAVKETILQNALKDSGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1D6</accession>
    <name>A0A9W8Y1D6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amine oxidase</fullName>
            <ecNumber evidence="9">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_010139</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="9">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <text evidence="8">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="8 9">Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the copper/topaquinone oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="9"/>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1D6"/>
    <dbReference type="OrthoDB" id="3341590at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009308">
        <property type="term" value="P:amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.20">
        <property type="entry name" value="Copper amine oxidase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049948">
        <property type="entry name" value="Cu_Am_ox_TPQ-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000269">
        <property type="entry name" value="Cu_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015798">
        <property type="entry name" value="Cu_amine_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036460">
        <property type="entry name" value="Cu_amine_oxidase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016182">
        <property type="entry name" value="Cu_amine_oxidase_N-reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015800">
        <property type="entry name" value="Cu_amine_oxidase_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015328">
        <property type="entry name" value="DUF1965"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638:SF20">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638">
        <property type="entry name" value="COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01179">
        <property type="entry name" value="Cu_amine_oxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02727">
        <property type="entry name" value="Cu_amine_oxidN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09248">
        <property type="entry name" value="DUF1965"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00766">
        <property type="entry name" value="CUDAOXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49998">
        <property type="entry name" value="Amine oxidase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54416">
        <property type="entry name" value="Amine oxidase N-terminal region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01164">
        <property type="entry name" value="COPPER_AMINE_OXID_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0186">Copper</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 7 9" id="KW-0801">TPQ</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040766039" description="Amine oxidase" evidence="10">
        <location>
            <begin position="20"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase N2-terminal" evidence="12">
        <location>
            <begin position="89"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1965" evidence="13">
        <location>
            <begin position="251"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase catalytic" evidence="11">
        <location>
            <begin position="331"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="404"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via topaquinone" evidence="7">
        <location>
            <position position="492"/>
        </location>
    </feature>
    <feature type="modified residue" description="2',4',5'-topaquinone" evidence="8">
        <location>
            <position position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF09248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4363021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="784" mass="87973" checksum="EF33D01F63AADF2A" modified="2023-11-08" version="1">MHFPTFTYLALTSVAISSAVPPLGYDRRVLRDIFDKRENNETTSSCLTEAALTTKAPKINPWAGLAPEENLAVWNLLHDPASGLNLTDPTEAKLTDNYVFWVDALHTNKSDVLPYLDEGAPKPSKYARAIIFEGGKAEPDSQEYMIGPLPVSAETTIEKLDYMYNGGSGGSVPFNARYFDGPRSTATDPLIASIMSNISDITAALFQGAAYYGSTDERSNLTLTSGTPLSFDGTSAFRSIMFRFPGPASYMTPLDFFLLIDCTGTDASLYFLKGFVTNEKFFPTIAELRAAFEAGELAMEYDQTLDADWALVDYKPELGVRDLEERLAPSTLEIGGKRYKLDEENRYVEYMGWSFYLTFSRTLGIMFYDIKFKGERILYELSMQEATAQYGGNQPKAANTVYHDTYYQLGTEMGSLLEGFDCPWGSTFWNITYSSYNTTEVNTNSICIFETDMNFPLARHRTGASNDYGFSNLGSVKGAALIVRAIATVGNYDYMFDYSFHMDGSLEVIVRASGYLQSSFYYPDQGKFGPRIQQATQGSLHDHIVTYKADFDILGTPNSLQVSELKAVNQSQPWFPELGTFEQMEMDIHNMQEEQQFNYAANNQAMYVVLNPNATNAWGEKRGYRIVPGRSDIHLSTLNSPWSLKNSEFAKSHLAVTRQHDNEPFANSVQNINLPWKPQQDFSKFFDGECVDGEDLVVWFNLGMHHFTRSEDVPVTLYTEAYSSIVFAPQNFFDRAQDGDLLNRRWVQIDDEGKMTFEDYGVGLDVCPIVLEEPTELLGGVGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1T4</accession>
    <name>A0A9W8Y1T4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Alcohol acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ATF1</name>
        <name evidence="1" type="ORF">N0V83_009660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1T4"/>
    <dbReference type="OrthoDB" id="2150604at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052058">
        <property type="entry name" value="Alcohol_O-acetyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010828">
        <property type="entry name" value="Atf2/Sli1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037:SF1">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07247">
        <property type="entry name" value="AATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4363368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="498" mass="55748" checksum="FD47573ECD5CA7B5" modified="2023-11-08" version="1">MTKKKVRERAKMVDITSLEKLRPCGRLETYSTARHYLGFYKSVGLTAAYTSYATVSTSLEKQIFEALRKVISKHPNLSAITVNEEKSYPEVYFARLPEIDLRTCVEFHERVRPFPKNGEADEELDALLAQQHSRDFKDEYGRNPFWRLVVVTSPDRPNEISVSWFFHHALADGTSAFLLHKSFLDALNSVAPEAEANPIVSSPKSDLVPSLEELHPLPISWPFLLHAILGLILPSVFNKRPAKLWTGNHVPVEVTTTPKLNYRTHVVSIESTKKLAQISRKEKTSVTATLQCLIAASLFASLPLAEWEKIKIECPISMRRFLNGVAEDQMTNTITQYGYLHERHTTTPSNGTAEVDVLQYFSWDEARAVKSAIQAEVAKEGCDNPIALLKYVSDLPQFFKKKLGKPRNPSTEFSNIGVYKNKEFGNEGSWKIGRMTFSQSPNLTSCPFCVNAVTGGDGNVVINFCWNQGAVNEAVIVQVIEGVAKGVEHLVAIHDTVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1X3</accession>
    <name>A0A9W8Y1X3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Nucleoporin protein Ndc1-Nup</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">N0V83_008680</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NDC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1X3"/>
    <dbReference type="OrthoDB" id="67850at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070762">
        <property type="term" value="C:nuclear pore transmembrane ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106166">
        <property type="term" value="F:spindle pole body-nuclear membrane anchor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070631">
        <property type="term" value="P:spindle pole body localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019049">
        <property type="entry name" value="Nucleoporin_prot_Ndc1/Nup"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13269">
        <property type="entry name" value="NUCLEOPORIN NDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13269:SF6">
        <property type="entry name" value="NUCLEOPORIN NDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09531">
        <property type="entry name" value="Ndc1_Nup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="10" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="261"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ4365064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="641" mass="71830" checksum="BDC2035C1D63E8F6" modified="2023-11-08" version="1">MALVPLGVQVRPYRDYLTPALHRRFNKASRYTLLLCYAIACWMGEWDNPLWLWFPIGPTGIRTLLLFLPALVIYVLRVAQWHVGRRQTLTQFDTFRKYFLRKSTLLTLVFYAFSAWLYSEVYIWSRTSKDKLGLTEYGRAHERLKLNERPLFLRYLFFLLAVAQSGIHLWKDYDKIEVPAMKPKKEREDAAAPAALRLSPVPRQVLATHLLAMVTRASILSAIVVTGGLVLYFAGPRYVIWDYYYSFSKYFVSLSKTSKPTGLAPFLPLCAMFVVEGTLLVLLWEFVNKAFDLYIAQEPLKNAQPITADSKDPNGTLLNGLKSKKEAVKAIAFWELALITDAFPDRRKTIYGEIERKKAPTYQQVTDICLAEIKLLIERISRGLDPAYNPGTAPGKQQPTAPVNLVPQISQPLKEDKLIAAPPPKPDTKWDHIESAATGIAKSYSAPGNSQQAYGREAINKSVKKAQEGAEQAASFATLYYNKLVKSPLGAPFSLSFPRTASLIVLGAPFSQISLICNAITALTNLTVFSLSQDSLGRFHEGVPAIIRVFTTAINQIDEYMNTTQIHWSDKKTLSKPEAARRKVPEVEVVRECLRGGLERILGNFNEYLGGMGLSKVEVLEAKKVAGVKKGPEMLQASGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y247</accession>
    <name>A0A9W8Y247_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF654-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y247"/>
    <dbReference type="OrthoDB" id="205993at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006994">
        <property type="entry name" value="TCF25/Rqc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684">
        <property type="entry name" value="NULP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684:SF0">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT TCF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04910">
        <property type="entry name" value="Tcf25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="569"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="579"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="687" mass="76729" checksum="505B79D01A63A439" modified="2023-11-08" version="1">MSSRALRRAQRELEEKQIQEKLAREAQDEEESDEEVAPKAKAKPSLFAMLGDRDDDDDEEDEEAEHEAGGAAEVASDQAEAEQAPTSSSSKKSKKKKKKKAKAKAVSSKPETNSATTKTQSGLDEIDQALLALNLTTSGRTGANSPHEGSGISEEKQQLFSVLSVDTQHLHAANEMKKLFGRAALQNTDEEQQRARQRGQGQQGGIAAATAGRNAPGNRNLTSIVLRRNIFVQGKEEWPRATSGGLGMEIVERRHDGSVEYRFVHNSMYQEVQRQFQVCVGSMDPERMVQLLHFNPYHISTLLQVSEIAKQQRDNATASELLERALFTFGRSIHSTFSSNLAAGKARLDFRRPENREFWLAVWRYISTLGVRSTWRTALEWARLLVAMDPEQDPYCIRLLLDQLALRGRDPQALVDLVEADYLQRLWKIPPNLAFSVALAHNRLKQPQKARSTLRNAIKEYPWLAARLCKELDISPIPKPVWGKEPNDDHQELLCQLYVPKAKDLWNTTEGTGLLVEVCYSFEEELGAGEDPYWLAQIPELDLARHVILSDDRSLLTLLDTRVKSKFTSVSDPLPPDDNLTSYDASTAGSSQGHRRSGAVRQELLAELEQLRTYFQGIDIENLVGGGLTEDDLIQALEQAGSSLEEFRRQSERFQVVRMGLQEEGVQVVFNGDPEGLGAGTGSDTDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2D8</accession>
    <name>A0A9W8Y2D8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_009591</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2D8"/>
    <dbReference type="OrthoDB" id="413460at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000019">
        <property type="entry name" value="DNA excision repair protein ERCC-6-like 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029256">
        <property type="entry name" value="Heliccase-ass-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057931">
        <property type="entry name" value="RHH_ERCC6L2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25806">
        <property type="entry name" value="RHH_ERCC6L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14773">
        <property type="entry name" value="VIGSSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="276"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="651"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="54"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="144"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="897"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="968"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="98"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="229"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="969"/>
            <end position="984"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4364136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1080" mass="122599" checksum="654747009327C99D" modified="2023-11-08" version="1">MSFSRNQARGHRRALRAFAESEAKSDSDDEDDLDIIRSTRKGLDLSQYIDGLTQTQTQSQDADVEHNTQNQEEEITDGDVQAVGPSRPRGKVVSSGTDEFDDAEKDLESDWSDEDTRALFRDFKNSKTLKRKRVAAETNRIRAKKKTKTTTTTSIVGRKPQPKKNAGHGPTGFSTDSVAEDADTTATFLDDPIPEYFLARQSNLKKLHEAGLRCPPSYRDVDFSDTETEEKPKLDKAIKPQRPKEDITLHESGGVIPAPIARWLRDYQVEGVQFLHERFVKQTGAILGDDMGLGKTIQVIAFLTAAFGKTATKRDAKCMRKIRRFGDGRWYPRVLIVCPGTLMQNWEDELSKWGWWEVYRYHGSNAADRKGVLGAAAKGMLEIMITTYTTYRNNESEINTVDWDCVIADECHALKGKNAEITKAMNKINALCRIGLTGTAIQNKYEELWNLLNWARPGAYGSAQEWKQTISIPLKLGQAHDATNAQLADSRSRAQELVHKILPSVFLRRMKTLIADQLPKKSDRVIFCQLTETQADAYRSFLESDRCEFIRTAKEPCDCGSGKPRGWCHFVNVPGENEKWARFMFPCMVTLQKLANHLALIVPVSTDNKDKQAKDMQTLEIACPDTFKDLFRIRDNILNQSQREFCGKWKVLRRLLDFWHANGDKVLIFSHSVRLLRLLRGLFDVDGTKYNFSYLDGSMKYEDRSKAVADFNADPNQFVFLISTKAGGVGLNITSANKVVVVDPNWNPAYDLQAQDRAYRIGQTRDVEVFRLVSSGTIEEIVYARQIYKQQQANIGYNASEERRYFKGVMDQSSKKGELFGLENLFTFQENSMLLRDIMHKTNVAETKAGVNAHDFQFDESQLDSDDDDILSNKSLGIIDDDANLAEIKKLAESFTSVSTTDTKSSRGRKKYTKRTGPDPINAILAKAGVQYTHENSEVIGRSEIEARLGKQAMELRNDIDLATKRVFQGSQSQSQSQHHSYQQNHDAAEFSPEDDDDEGDVRLLGSATKGEFKINYRYRPDERIRRRQFCSMAKSMGFADPVEFALLVESSTQDERRKMLERFYRGRRREMMGEFGGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2X3</accession>
    <name>A0A9W8Y2X3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DDHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007915</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2X3"/>
    <dbReference type="OrthoDB" id="431378at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004177">
        <property type="entry name" value="DDHD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058055">
        <property type="entry name" value="PA-PLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055555">
        <property type="entry name" value="PA-PLA1_DUF7131"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057826">
        <property type="entry name" value="WWE_C20G8.02"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509:SF10">
        <property type="entry name" value="LD21067P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509">
        <property type="entry name" value="PA-PL1 PHOSPHOLIPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02862">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23465">
        <property type="entry name" value="DUF7131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23463">
        <property type="entry name" value="WWE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01127">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51043">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDHD" evidence="2">
        <location>
            <begin position="661"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="816"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="821"/>
            <end position="842"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="989" mass="110291" checksum="14D8343D4267617E" modified="2023-11-08" version="1">MSAPDNKQGFLSNLSPWGSRAATPKPPSAPKPVEEGADKHKDKGKDPEKGAASLAPQQGGDHVVDRRHRLSLKRYPQDCPPLAVRWFHAIDLPKRKPLSAHAIAPDKPLPRPKKWIPFSASDSRAIEAAFQKTADEDEAAELRRGISNPTTPTRSNAGPTTTTKVPVNEDYLFDVEIETRELAPAYWLGPVYDVKRGTWFTPDGEAVDESLATQLEEGYLKLKPWRFGKPEEKRSASQTRGRPLSMGPSVGSDHRKELAKLNRDSTSNPVTPKSSFGSLRNEAHRSQEDAKPDDAPATAPADSPHMYRLFGAYMNSTVTYQDANTAWLLTDDMWTRMGSTLYQRFAGGAHYAGYKYIRGYTDPKDKRKAPAVKDPTSDKQAEPTTTPSMAYGSDHSNPSTATASETNSDGETSDNETGRESPSQTRRRNLERQVSQLMTSSKPEYEQKQEEELRKREEKEMRDDYKSQDKNDQGREIEHLLLITHGIGQRLGMRMESINFIHDVNMRKSFKSTKNNRIQVIPIVWRHLLDFPKQSLKHNRKEHDLGDLDHEDHEYPNLEDITVEGVPAVRNFLTDLALDILLYQSPAYKGHISRIVVNELNRTYRLFKDRNPSFKGKVSLVGHSLGSAIMFDILCIQKDAKSRSGSHSSKHRRSTEEGLKLDFEVEDFYALGSPIGLFQMLKGRTIAARPSASFVPPKTPASPLDDPFSEAATEYDQHAFDITTSSPLCKQIFNIFHPTDPISYRIEPLISPAMSTLKAQPLPYTKKGIFGAPASQGLTGIGARVGQGVTDFWSSLGSGIASGLLNRSLGISGTEMAGGSSIPQGQRTSRPLSVGPSQNASTGVVPGLNEPASAFLNEERRRRLGQETIAEGEDGEHPPTLIESEIETLFAGFQKRRKSQQSEDGTRDVEKDLEWQELEERSRKLKKEEMKVRSLNSNGRVDYSIQEGAFDISLLASIASHLAYWADEDVSHFMISQLLARHRVFKPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2Z5</accession>
    <name>A0A9W8Y2Z5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Nucleolar protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NOP15</name>
        <name evidence="7" type="ORF">N0V83_008943</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2Z5"/>
    <dbReference type="OrthoDB" id="21467at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12307">
        <property type="entry name" value="RRM_NIFK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46754">
        <property type="entry name" value="MKI67 FHA DOMAIN-INTERACTING NUCLEOLAR PHOSPHOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="232"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="406"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="168"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="429"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="459"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4365323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="539" mass="57687" checksum="24BC87A42C5B671F" modified="2023-11-08" version="1">MAAEDTKGKKRKGAADSAPKAKKARKSDDVAAAPSKPAKGTKKTPATAPVPTETKPAKSKPARKEAADFFEADEAPATEPAKLTKGKKGKAASKTNGDVTEPVAAEADAPKAKTKAKKGKQADAAPVVEDVPAEAQVEASGKKSKKAKTGKKQEEKEEEVAEVAPASDEVEDDIEEDDQTAALLAGFESEGDSEDPDEDLDFDDDVHVPALTKSQKKAVEKAEQANKSNSPGVIYVGRVPRGFFESQMKKYFSQFGRVNKLRLSRNKKTGASKHYAFVEFASTEVADIVARTMDNYLMFGHILKCKLIPAEQVHPDLFKGAGQRFKVDPRNKKAGLEMERGVERAQWEKRVEKENKRRHGRNKQLKEEFGYEYTAPEVKAVDGVPKQAAALDNAASQQLLSEAAAQESALVEEQPKGKKGKKTAKAQEEVQAAEAVEPAPAKKGKKAAKGKAADETNGEVEAAAEVAEPVAEKKGRGRPKRKPEAALDAAAATEDVEAAPKSKKAKGAKADTANVVVKVKAKLEQEAVAKPKKAKKAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y384</accession>
    <name>A0A9W8Y384_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein phosphatase 4 core regulatory subunit R2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPP4R2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y384"/>
    <dbReference type="OrthoDB" id="341898at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030289">
        <property type="term" value="C:protein phosphatase 4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015267">
        <property type="entry name" value="PPP4R2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16487">
        <property type="entry name" value="PPP4R2-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16487:SF0">
        <property type="entry name" value="PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="324"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="330"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="360"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="40582" checksum="293C9B2B2CF8B676" modified="2023-11-08" version="1" fragment="single">IVSEFPKPPAEADSTPQTSTELPSRPQTSNAISSQESHTTDKENAPPATPPRPPVPAFEAPSDSQHVPQDITTFYSSIRNTLATNFAQHPPHTVQRLAELILEPQRRYRYLPPYLRALDRVVSVSSPLTIFPLPQAVLPTAGGLLNGTTSANAPQTTALGSDESLGGALLTPIPWLQNRGQNELISESTEMVDGPNGAGRIETVTVGMLSGNARPESAQTSQIASSHPDGETLPSTGPVTQGELLRQEQEAGIVLNNPHSMTTSPTRTTFGEIEGGGTIVETVEGVEEAPHARGPEIIGLEDTGLQKQGGRLDIEGAIGRPSVVQANKSPAPAEPAQGEAQDENMADASGKSETAPTEKAVTRRDTAAKEEAKKSQDVDMKADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3N8</accession>
    <name>A0A9W8Y3N8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">endo-polygalacturonase</fullName>
            <ecNumber evidence="3">3.2.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">N0V83_007323</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(1,4-alpha-D-galacturonosyl)n+m + H2O = (1,4-alpha-D-galacturonosyl)n + (1,4-alpha-D-galacturonosyl)m.</text>
            <dbReference type="EC" id="3.2.1.15"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.15" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3N8"/>
    <dbReference type="OrthoDB" id="1546079at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050434">
        <property type="entry name" value="Glycosyl_hydrlase_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31884:SF9">
        <property type="entry name" value="ENDOPOLYGALACTURONASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31884">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00502">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 15" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="active site" evidence="14">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4366798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="212" mass="22445" checksum="CDB486AC043E135B" modified="2023-11-08" version="1">MRGSHNEDLPSDSENNQLIIKPLLGFDLASTNDTKVRDSVVINQDDCVAVTSGNNVIVNNMYCNGSHGLSIGSVGGKSNNNVTNISFTNSVVLNAENGARIKTNSNTTGFISNILFENIRVENISIYGIDIQQDYLNGGPTGSPTNGVIIQDIVIKNVTGTATEEGRDYYILCGDASCSNITINDVSITGGGVPSACNFKTNGNFDCDGGFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3Z1</accession>
    <name>A0A9W8Y3Z1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mitochondrial Translation Optimization</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MTO1</name>
        <name evidence="8" type="ORF">N0V83_007610</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the MSS1-MTO1 complex that catalyzes the 5-carboxymethylaminomethyluridine (cmnm(5)U) modification at the 34th wobble position (U34) of mitochondrial tRNAs.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MnmG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3Z1"/>
    <dbReference type="OrthoDB" id="3329at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070899">
        <property type="term" value="P:mitochondrial tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000145">
        <property type="entry name" value="tRNA uridine 5-carboxymethylaminomethyl modification enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.570:FF:000001">
        <property type="entry name" value="tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000002">
        <property type="entry name" value="tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.570">
        <property type="entry name" value="GidA associated domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00129">
        <property type="entry name" value="MnmG_GidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049312">
        <property type="entry name" value="GIDA_C_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004416">
        <property type="entry name" value="MnmG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002218">
        <property type="entry name" value="MnmG-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020595">
        <property type="entry name" value="MnmG-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026904">
        <property type="entry name" value="MnmG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047001">
        <property type="entry name" value="MnmG_C_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044920">
        <property type="entry name" value="MnmG_C_subdom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040131">
        <property type="entry name" value="MnmG_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00136">
        <property type="entry name" value="mnmG_gidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806">
        <property type="entry name" value="GLUCOSE INHIBITED DIVISION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806:SF0">
        <property type="entry name" value="PROTEIN MTO1 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01134">
        <property type="entry name" value="GIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21680">
        <property type="entry name" value="GIDA_C_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13932">
        <property type="entry name" value="SAM_GIDA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01228">
        <property type="entry name" value="GIDA_assoc_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01280">
        <property type="entry name" value="GIDA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01281">
        <property type="entry name" value="GIDA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA uridine 5-carboxymethylaminomethyl modification enzyme C-terminal subdomain" evidence="7">
        <location>
            <begin position="584"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4367080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="692" mass="76214" checksum="589ED1CFAE50B24A" modified="2023-11-08" version="1">MQRVVPQRARQLWGHAPRVRSVNRSRRTFATVSGGERPYDVIVIGGGHAGCEASAAAARSGARTALITPSVHNLGVCSCNPSFGGIGKGTMLREIDALDGVVGRIVDKAGVQFRVLNRKKGPAVWGPRAQIDRSLYKKYMKEEMLSYSGLSVVEGSVADIIVDRTQEEGVGGKGRYGKITGVRLESGEIIPTGNVVITTGTFLGGEIHIGLEAYPSGRMGEAATFGLSKSLRDAGFTLGRLKTGTPPRLDGKTIDYKVLEAQEGDDPPMPFSYLNDRVQVEDQLLNHETRTNEATHDIIRNNLDKSIHIRETVKGPRYCPSLESKVIRFTDKKSHIVWLEPEGFNNDTIYPNGISCTVPEDVQLAMLKTIRGLENVTMLQPGYGVEYDYVDPRHLRSTLETKNISGLFLAGQINGTTGYEEAAGQGVIAGINAGLKSLGRDPMVLTRADGYIGIMIDDLITKGVSEPYRMFTSRSEYRMSARADNADTRLTAMGRAAGVISDKRWTAFEREANEMADLTKIMQEKTLGWTDWNKAGFSVRNDSTKRSAYDLLRLPNTTPSSLTHLLPGISGFSQRIQDRVHIEATYAPYVAYQTISQARWFKDENLKLPEDLDYESIFGLSFEEKRALEVARPESVGMARRVEGVTPTGALRLLQYVKGAGREERNARLKEEVELRKAKYMNATPSLGVEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3Z3</accession>
    <name>A0A9W8Y3Z3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3Z3"/>
    <dbReference type="OrthoDB" id="6020543at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4366424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="170" mass="18594" checksum="03A56BDF47157371" modified="2023-11-08" version="1">MDPVEPVQPFPNLFDTSHPPSAFALTIFSLFGEGHTERADLRPLGPDVSLASTTAAAAAAQRRRSAALILDRLARPLGEEVVVDGYDVQVPAQWKGTYQDSQFKTLVASLRELNRQAWTASGGVEGGPNDLGADGRGVVFFEWVGEMLKREVVTEVVREGWVEWDGQAWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3Z7</accession>
    <name>A0A9W8Y3Z7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_008940</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3Z7"/>
    <dbReference type="OrthoDB" id="5304367at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4365320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="144" mass="15812" checksum="CA33EC832B91FD11" modified="2023-11-08" version="1">MGGRLSMPKGPDGMSETSKPTSANAAARLSRNKGIINGRTVMAPLAALTMAGLLFVYARTSIRAAKLNAQKHREADGGQISWHKESMRRHGHLEKVEDDRGTLKEALMGDIRKKNKETGGESEKTPVERSDNDQELRKIMGKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4P5</accession>
    <name>A0A9W8Y4P5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Spo12-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007431</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4P5"/>
    <dbReference type="OrthoDB" id="5578329at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007727">
        <property type="entry name" value="Spo12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05032">
        <property type="entry name" value="Spo12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="113" mass="12472" checksum="9725D99D74E02577" modified="2023-11-08" version="1">MSSNVLSVRDNNAQIKPTFSPEKEKPKSLEYHRQVLQSRMDSEQTQPYVSPSDEIMSPATQKLQAFKHKHAMKKSKPATLFKKTSSKNFESAKGTAMFADIPKATNGSKGAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4T3</accession>
    <name>A0A9W8Y4T3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_007269</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4T3"/>
    <dbReference type="OrthoDB" id="2213137at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF315">
        <property type="entry name" value="TRANSPORTER MCH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="87"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="413"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="450"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="5">
        <location>
            <begin position="291"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4367684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="523" mass="56140" checksum="3D3D984E87516297" modified="2023-11-08" version="1">MTTLPPRHHHGKEDEDSGEELGVARSDEKSMNSLEASNGGITNGPVSKEQHHAAPANGEDKGIASENAAAAAAAKKTPSDVPPNGGYGWVCVAACATINAHTWGLNSAYAVFLAYYLSNNVFPGATHLEYAFIGGLSISQALIVSPIATLTTRRFGTRTTLLIGVVFEAVSFISASFASHIWHLFLSQGICFGFGMGFLFVGSVGISAQWFTTHRSLANGIASAGSGLGGLMYSLAAQAMIKNIGLPWAFRILAIIAFVVNTTCALLIRDRNKQIGSSMLSFDYTLFKRVEFLGLMGFGFLSMLGYVVLLFSLPNYARTMGMTAKQGSIIGAILNLGQGLGRPPIGYFSDNIGRLNMASSLTFLCGLFCLVIWMFAKSFGVLIFFSLIGGTVAGTYWAVVAPVTTEVMGLKNLASALSITWLVLVLPTTFSEPIGLEIVAFNGGSYTGAILFTGWMYIGGAICLWLVRAWKIGEMEWEAALAGKDEQEIDPLADRPTVSEGMVSLHNFKRSPFVKRMFMWQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4W7</accession>
    <name>A0A9W8Y4W7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">C6 zinc cluster transcription factor-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">TFC7</name>
        <name evidence="1" type="ORF">N0V83_006371</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4W7"/>
    <dbReference type="OrthoDB" id="414418at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4368016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="127" mass="14056" checksum="371EA8143246A05F" modified="2023-11-08" version="1">MIVELHERVRRALDHIITTLDNEPEQPKTVLLCTHAATIIAAGRVLTGQMPDDPDEDDFHCFTAGLSKFVRRDADPSKGVAGNWDCVLNSDTSYLSGGAERGWHFNGEESFVSFAEDPKDDKEAPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y514</accession>
    <name>A0A9W8Y514_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20 21">NADPH--cytochrome P450 reductase</fullName>
            <shortName evidence="20">CPR</shortName>
            <shortName evidence="20">P450R</shortName>
            <ecNumber evidence="20 21">1.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">cprA</name>
        <name evidence="24" type="ORF">N0V83_006431</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 20 21">
            <text>2 oxidized [cytochrome P450] + NADPH = 2 reduced [cytochrome P450] + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24040"/>
            <dbReference type="Rhea" id="RHEA-COMP:14627"/>
            <dbReference type="Rhea" id="RHEA-COMP:14628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:55376"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="1.6.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="20">Binds 1 FAD per monomer.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="20">Binds 1 FMN per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="20">Endoplasmic reticulum membrane</location>
            <topology evidence="20">Single-pass membrane protein</topology>
            <orientation evidence="20">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Mitochondrion outer membrane</location>
            <topology evidence="20">Single-pass membrane protein</topology>
            <orientation evidence="20">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Cell membrane</location>
            <topology evidence="20">Single-pass membrane protein</topology>
            <orientation evidence="20">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the NADPH--cytochrome P450 reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="20 21">In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="20">In the N-terminal section; belongs to the flavodoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.4" evidence="20 21"/>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y514"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003958">
        <property type="term" value="F:NADPH-hemoprotein reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06204">
        <property type="entry name" value="CYPOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.990.10:FF:000009">
        <property type="entry name" value="NADPH--cytochrome P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000100">
        <property type="entry name" value="NADPH--cytochrome P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000111">
        <property type="entry name" value="NADPH--cytochrome P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000024">
        <property type="entry name" value="NADPH--cytochrome P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000018">
        <property type="entry name" value="NADPH--cytochrome P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03212">
        <property type="entry name" value="NCPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023208">
        <property type="entry name" value="P450R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF17">
        <property type="entry name" value="NADPH--CYTOCHROME P450 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000208">
        <property type="entry name" value="P450R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 20" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 20 21" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 20" id="KW-0274">FAD</keyword>
    <keyword evidence="3 20" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 20" id="KW-0288">FMN</keyword>
    <keyword evidence="2 20" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14 20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 20 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="15 20" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 20" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="9 20 21" id="KW-0521">NADP</keyword>
    <keyword evidence="12 20 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 20" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="18 20" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="13 20" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="17 20" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="22">
        <location>
            <begin position="85"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="23">
        <location>
            <begin position="296"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="91"/>
            <end position="96"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="142"/>
            <end position="145"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="471"/>
            <end position="474"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="489"/>
            <end position="491"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="506"/>
            <end position="509"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="572"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="634"/>
            <end position="635"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="640"/>
            <end position="644"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="676"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="714"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRNR" id="PIRNR000208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ4368076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="715" mass="79391" checksum="75C88D2378EDBAA2" modified="2023-11-08" version="1">MSAQPLSIPLTLPLTPLSKMAQLDTLDIIVLAALLLGTVAYFTKGTYWAVYGDPYGSSLANANGAARAGKSRNIIEKMEETDKNCVVFYGSQTGTAEDYASRLSKEGHSRFGLKTMVADLEEYDFDNLDSFPEDKLAVFVLATYGEGEPTDNAVEFYEFIGSDDISFAEGGSVDDKPLNKLHYVAFGLGNNTYEHYNSMVRNVDKYLTKLGATRLGAAGEGDDGAGTMEEDFLAWKEPMWAAVSEKMGLEEREAEYEPVFEVIEKPELNAEDDTVYLGEPNKNHLEGTQKGPYNANNPFIAPIAESTELFTAKDRNCLHMEISIAGSNLSYTTGDHIAIWPNNAGKEVDRFFKVLGKEDKRHTVIVVKGLDPTAKVPFPSPTTYDAAVRFHVEINAAVSRQLVSGLAQFAPTEDIKAEMVRLGGDKDYFKQHVTDRNLNLAQLLEIAGKGQIWSKIPFSFMFENLLKIQPRYYSISSSSLVQKDKISITAIVESLEKPGAPYVLKGVTTNYLLALKQKQHGDPNPDPHGLDYAITGPRNKYDGIHVPVHVRHSNFKLPSDPSKPIIMVGPGTGVAPFRGFIQERAAQAKAGQNVGKTILFFGCRKQSEDFLYQNEWKQYKEALGDNFEIHTAFSRDGPKKVYVQNKLEEAGEEVNKLLEQKAYFYVCGDAAHMAREVNTLLGKIISKYRNVTETKGEEIVKAMRASNQYQEDVWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y543</accession>
    <name>A0A9W8Y543_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tubulin-specific chaperone D C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_006461</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y543"/>
    <dbReference type="OrthoDB" id="10253476at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048487">
        <property type="term" value="F:beta-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058033">
        <property type="entry name" value="ARM_TBCD_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033162">
        <property type="entry name" value="TBCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022577">
        <property type="entry name" value="TBCD_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12658">
        <property type="entry name" value="BETA-TUBULIN COFACTOR D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12658:SF0">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23579">
        <property type="entry name" value="ARM_TBCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25767">
        <property type="entry name" value="ARM_TBCD_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12612">
        <property type="entry name" value="TFCD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin-folding cofactor D ARM repeats" evidence="4">
        <location>
            <begin position="341"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="356"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin-folding cofactor D C-terminal" evidence="3">
        <location>
            <begin position="927"/>
            <end position="1106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4368106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1183" mass="130280" checksum="620089DC15AA42C1" modified="2023-11-08" version="1">MGPGEDDDLKLQMASASLLADFNQLLPRLLRRRSRDGTPGRIRQQVRDVDMYKACALIEPFQEDPQILDSHLQAFIPPLVDAYLEAIQIASKEEPKKGFVPLSHAICYLVNVFCKVRGEKVIKGFLNNEPRYLELILNELESRGHLHAKDDEGNHEYLVFPWVERYVLLLWLSHLLLTPFPLDSISGLQQSAQTSTDLGIDLTHGVPEITLRVLTLCVQHLQSASKERGAAASLLVRLSIRPDMQKVGLLKSLVKWSLTFFLSVSEKKVDIHKSLGVLSYLSGLVASATNEEVGPFLAAIYRCCRSILNQECLAFIKSSAVARKLVIKTLRNIVLHCLQATSTTSELDPTSTVEEVIEYLLEALADGDSPVRYGASKALSVITLKLDLEMADEVVVAILDSLTENVYWENTERNLNSVNPLRWHGLTLTLSQLLYRKAISTNHLPNALDALLLSLGFEQRSPTGGSIGTNVRDAACFGIWALSRRYGTADLLAVSTASIRVSKKRKDISVPQLLAIELVVAACLDPAGNIRRGSSAALQELIGRHPNTIAEGIPLVQVVDFHAVGLRQRAVCEVALKAGKLQPLYWEALFENLLEWRGTAGLDSDSRLFAAKAVGLLSKFQPPGIIRQMADQVRSKLAALKPREVEERQGLVSALAALAYTVSLVGSAGNCYAPQSALSHLWTLLDEELKLEGKAFTSPSLRPEFTASAVCNFIGAMAALTTRLPEDCTPSDLQTKEVIRILSLCLARHEDTVLEAVKEATPAILRLLRRRTNTGTDELLSVWSSKLETVQSHNGLRCSGHAIALGAAYALLYAGHADTKESGLQTRIIEVLTFRCTPAVAIEARILALRALGILLSNGRSEAASTSIFLAAGLEAQVGSAIHIALNDYTITERGDVGSLVRLEALNTVLNAWNSGILRNVEKETGQKIYADVLRLSLEKVDKIRLRAARLIQNDTPGNVVHAKTTTADDVSSRAYFARALEMLRSIHPDSNLKESICVGFVSAAGMGTESVVQNSRAALLHFVDTLPTEHTSDREQLTLIGLLGCMVNLLKLYVAEDRILLSSLEVVAFLFDMQVIQRLIPTSFNFRMLLSYTQKAHFKSTHMQKLHLALDVYRGLGTIPTTRVDTITKAMSMLLHPFPKIRIAAAETLWVLTQEQDLMLHDWSLSWKSLKPIVDSMRKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5A2</accession>
    <name>A0A9W8Y5A2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008503</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5A2"/>
    <dbReference type="OrthoDB" id="1022638at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843:SF2">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="2"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="247" mass="27780" checksum="BFB8F8FDBE39DF03" modified="2023-11-08" version="1">MSEIRVGEGDDVRSFHIHKDLLTSRSEFFANVLKGYKDSSKSGTHNDTFEIVHWKEGSEGIIELPKDEPEVFETYVQLIYQGAIPIRKKLTVCVPVESVDAELKAKSEKGIKEIVDYEYVLLAELYVLAEKLQDIAAKRSIFSAFIEATLIRANGRSYYPTLKATDIIYAGTSASDPMRAFIVDCFVFVGHTAWVEDTAAASYPQDFLFGVMVGMFKERHKPKDATRVKNATSYLSKLQPYVEKKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5J7</accession>
    <name>A0A9W8Y5J7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006514</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the avfA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5J7"/>
    <dbReference type="OrthoDB" id="10254604at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15020">
        <property type="entry name" value="FLAVIN REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15020:SF50">
        <property type="entry name" value="UPF0659 PROTEIN YMR090W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="2">
        <location>
            <begin position="9"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="278" mass="29723" checksum="A91685DF977DEA77" modified="2023-11-08" version="1">MGHSILLIGGHGKVSQLLTPLLLAKSWNVTSMIRTAEQKATIEKLGQGQPGNLSVLVSSVADVKSEADAKKVLDQVKPDWVVWSAGAGGKGGAEMTFAIDRDAATYFTKASIHTPSIKKFLTVSYLSSRRGRPSWWTDKDWEAAQKVNNEVLPTYYKAKVAADEVLTVYAKERNDEEAKSSVPVQDRFCGISLRPGTLTDEKAGGVTVGKIGAGGKTSRATVAEAIVAALGTEGAKGWIDIIDGDDDVSTAIEKLTKEGVDSVDEEDLETMKENVAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5T0</accession>
    <name>A0A9W8Y5T0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_006358</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5T0"/>
    <dbReference type="OrthoDB" id="3364175at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052783">
        <property type="entry name" value="Metabolic/Drug-Res_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47655">
        <property type="entry name" value="QUINIC ACID UTILIZATION ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47655:SF2">
        <property type="entry name" value="QUINIC ACID UTILIZATION ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="3">
        <location>
            <begin position="40"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="371"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="371"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4368003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="526" mass="58374" checksum="F301ECA27E8034D3" modified="2023-11-08" version="1">MQCWLPICEKLDVLKLSYSYPAEGLVLSQDMPGSGYHAEMWSIFAVGCTQDISGTLGAHQHYHITTAKKLYDVARMLIPNELGKFDLNHIKALLNLAIFNMSQSHLNAAWLLVGAASRIFLTLDESSEATSPRRKNVWAGCFLLDNLLALHLNQRPYLEKSDFTWVGKIEEDGMEEWQPWSGDLIPGPASQSRLPTLALSTFNNLLEIVDILVSTARKSAARNFLHEMIGRLEMWKSALPPKFDYIRVDSASTPLTPPAVLLQLTYLATAFALVPSQAWLQRVLELLELLKDQFGFARLPSIVVCLLQNIKRCTANLAMDQGLRIRMHETFAEFDHAYAIHSGGLLLEPQRTPNALAYNSQNLIRMPNPEAIQTSPQSFTSQSGKPVTNPYQQPASSSTLLDDLLPDMHTNQQEHALPSLNSLPFNIANNAPPLEPGSLDAYDPYNAFISGDLDNFFDELASLHGAKKLRNQSQFMENLGFSSEASMADLLAADPGRFMPSPSANIASGNNDQSPNFAMDHFYEPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y624</accession>
    <name>A0A9W8Y624_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_006100</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y624"/>
    <dbReference type="OrthoDB" id="21449at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04369">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15398">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15398:SF4">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN 8 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="5">
        <location>
            <begin position="726"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="153"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="850"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="183"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="256"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="294"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="345"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="371"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="436"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="538"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="548"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="590"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="850"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="861"/>
            <end position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4369018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="880" mass="95583" checksum="BDF2AB64383380E2" modified="2023-11-08" version="1">MGSVSAYTNLESLLLFQCLHAYGQAPSVFGRISDLLKGNPDITGHKYFQAGRLSPDALRNFYLERLRRELEHEQATDDKGHSGDAANPRKRKLPTSSLPTVQEALQHQHLIPKLVTKLYASYRNEITEQIRAEEERYERLQREITSIQRGEWDDQLRERAKRSTPASHSPTVSKRSPHLAQKPLQPNTTPQLTPNGAQGGPVTAPTPDASQQPSLEPPRPPPSQNGQSSPSRGKKHASLDQRRAPSTPQPLAAPTPSHNETQPSGLSPRSQQSSNATYNSAPYTNLSPYPPVQQPQATHNIQSQPTPHQNHARPAHPQSPVVGSPGPQFQPPAIQPYSGSGVPQYGPPLAAPPHYSPTPQRFPHQPPPGSQSPGVQGPYQPQRAQYPQAGQPTYPLVQGQMGQPPPQAGFMLPPFQVSPQDPSRVHHQAAVPLQHPQVSTPANNRQHSRSNVQTPNAGRSGGPPMHPLVIQARQSVSTPMSARSPHSSLGTPVSAKSFWKRQSFGATPGTPASPRPIIEPIDDFQPLAQPLQSSPKAKSQRKTRAKGKGKEKGTAKDAEPDPAPDSDPAQEQKLLDAQEEDTPEVETRSGRSRRKAAPKRTRPGSIASSRAGGSGRDRSRSQSILSHTETVAADNESQAGHRIKSERGVSVDAIEEETVSTPSQVSTRRRGAAAQAPHSTRRKRNAREASLEEAEEHFTNTLGPPRSIIAQRHFSKMCAPIMNDISSHKHASTFTTAVRAKDAEGYYDIIKRPTDLKTIQKAIATGAKQVAAAASDTPVGSPGGGGAVIELPATADNMPPKAIVNAAQLEKELMRMFVNAVMFNPGEDGVVEDAREMFETVQRSVSNWRNVERSSGRTEVEDTPVAEEEEVAQVSKRRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6A7</accession>
    <name>A0A9W8Y6A7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphate transporter (Pho88)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PHO88</name>
        <name evidence="3" type="ORF">N0V83_007384</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6A7"/>
    <dbReference type="OrthoDB" id="18139at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012098">
        <property type="entry name" value="SND3_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28112">
        <property type="entry name" value="SRP-INDEPENDENT TARGETING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28112:SF1">
        <property type="entry name" value="SRP-INDEPENDENT TARGETING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10032">
        <property type="entry name" value="Pho88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008756">
        <property type="entry name" value="P_tr_PHO88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4366856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="191" mass="20807" checksum="1864FD97D58973A7" modified="2023-11-08" version="1">MAISPQISNLVIILGFMQISKKIPFDDPNVLNGVRALYVVSNLIIAGIYLYVQSQINKKNDMTVVKYVEPAPMGSGEEPKFVATTVKAYDLQKLREAFKAQLMGVGMMGVMHIYFKYTNPLLIQSIIPLKGAFEGNLVKIHLLGQSATGDLQRPWKASGGMMGAMSGGEIKTDKKSIEAAERAGRGGVKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y719</accession>
    <name>A0A9W8Y719_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_005928</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y719"/>
    <dbReference type="OrthoDB" id="655030at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051704">
        <property type="entry name" value="FAD_aromatic-hydroxylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46865:SF2">
        <property type="entry name" value="MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46865">
        <property type="entry name" value="OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="4">
        <location>
            <begin position="9"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4368846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="416" mass="46388" checksum="F6D676C333607068" modified="2023-11-08" version="1">MALSTLPKLKVLISGAGIAGPCLAYWLAKTRPNYSITVLERSPVPRATGQAIDIRGAAIEIIKKMKLEEAVRTRGTTEEGTRILSASGKTIAEFGKGDTFTADYEILRADLCGLFLEATENLKNVRYVYGDYVTSLQQTDKDVTVTYDGGSKEDFDLVVGADGATSKIRAMILDEQVRKDSYKFIGQYVAYFSIPRQSSDTKHWYWYNAPKGLALMTRPHRNPSTIGAYTCYTLPAHGQHEPAIEEALKQGPEAQKRIIHKYFDDAGWEAKRILDGMDKADDFYMSRAALVRLPKWTNGRTVVLGDAAFATFGVGTTLAIHSAYVLAGELSKVQSTDDIRRAVARYEEVFRPIYAKSEDPMPGFPQIAFPQTGWGLRVRDTLLWLVSRTKAYKLLPADKEENSPLPEYDWVGESRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7C1</accession>
    <name>A0A9W8Y7C1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_006344</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4367989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7C1"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000057">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF49">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G04250)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="39"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4367989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="234" mass="25693" checksum="A3CA3E8CD4C00183" modified="2023-11-08" version="1">MDDKNDVAMVEMIDKEDPLPPQLDPAAEKRMVRKIDIRLLPITAVVYLLCYLDRSNIGNAKILNSTTHDDMRETNNMTNYQFTIALMAFLIAYSIFEAPSNLALKVISPNRWIGMLVIAFGALCAGIAGTHNFAGVTALRFFLGASEAGSFPGLIFYFSLWYKPEERATRIAAFLCSATLAGAFGGALAYGVGHMNGVGGLEGWRWLFLLEGIITSKLILRLSCHGMFAESVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7G9</accession>
    <name>A0A9W8Y7G9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">(4-O-methyl)-D-glucuronate--lignin esterase</fullName>
            <ecNumber evidence="7">3.1.1.117</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_005634</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 4-O-methyl-alpha-D-glucuronosyl ester derivative + H2O = 4-O-methyl-alpha-D-glucuronate derivative + an alcohol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67452"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:171667"/>
            <dbReference type="ChEBI" id="CHEBI:171668"/>
            <dbReference type="EC" id="3.1.1.117"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:67453"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbohydrate esterase 15 (CE15) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.117" evidence="7"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7G9"/>
    <dbReference type="OrthoDB" id="3781271at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054579">
        <property type="entry name" value="GCE-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22244">
        <property type="entry name" value="GCE_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="4-O-methyl-glucuronoyl methylesterase-like" evidence="8">
        <location>
            <begin position="117"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4369870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="404" mass="43137" checksum="C5C67EA53C852A5D" modified="2023-11-08" version="1">MRTELIALVGVAIGTYGAPLEDIRLGQILDLLPRQSNVTCPQTPTPMPKAADLPAVKTMPDPFLYLDGKTRVQSKDEWVQCRQPEIMKLLQEYQYGYYPDHSQETVSATRSGNTLTVSVAAGGKSATIAATLTFPSGTGPFPAIINIGGMDSKTYLNAGIAVVSFDYGKVAADSNSKSGSFWTLYNGKDIGVLTAWAWGFHRVLDGIELKVPELDATKIGVTGCSRLGKAALAAGLFDKRIAVTMPMCSGVQGAGPYRYSLSGQGENLENAKSGAGWWTSSGISQFVGKSTQLPYDAHTIVAAIAPRAVILSQNSGDQFTDSKGTAEITFPAAKIVYSWLGAGEKLGMSIPSGGHCDQSGYADVLPFVQKVLQGKSTTRNYDDLKTWKAMPEAYPWGTDVPKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7H8</accession>
    <name>A0A9W8Y7H8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_006419</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7H8"/>
    <dbReference type="OrthoDB" id="3784116at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047499">
        <property type="term" value="F:calcium-independent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185:SF1">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5 6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="9">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="450"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="6">
        <location>
            <begin position="25"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="6">
        <location>
            <begin position="186"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="486"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4368064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1019" mass="113642" checksum="6E9E2DA8366C0D56" modified="2023-11-08" version="1">MEEISRGLPPNQVLLPCEYFDIICGTSTGGLIAILLGRLRLSIPEAIKHFNALSSKIFPGVKPTGWSSKIPFKDLWNKGKSVTGYALFDGKNMENVLQNFLATDLERDPQLPMIPLPDENADNCKTFVCATRNQSVDKILFRTYPTTDPSQADYPLKVWQAGRATSAAPLLFNPFTIEEYGVTVTDGALRLNNPIFEAMAEARRIDRNRSFGCVVSIGTGVADVRGIDTESIKMHEIAQTCVEIAINCDNVAREFVGSDEEGDLENSHRYFRFDVRRQLDAVDLVDWTRLNDITAYVDHYLSEKDTGDKLKLAPEFVYRYGQGQRTSRDIFYVQADLTEHARRDLDNIIGMLAPQTQLPRGTLTKVEELKALLEEPAVPPWVLIVDGTGRGSKVPIFPDSYLPNSDNGRVVMVKPDVRATQPPNGRTVPQELDVSVTKHIRYSSEDKLPISFQESIGLGGSGSVYPVTVSSKIGTLESTGEVFACKVVFSGRGHGESFQIELEVLKQVKHQHIVEVVISFEIEERIFQSGFLMRPKADGDLSHLLRDEEYGQSLIQPRWFACISNALNYLHGKLIVHGDIKPSNILYKDNEIFIADFGVSTIIQNQEAVNYVHAFTPGYAAPETFDSWTNSARRDRSLFLAESFSLGCTYFELLRKYLDAHLGRAVRPYSEVTPWMQSKLLDMEERLSNYPAEKNARLLAPIIEGTRGLIDSTASSRYKPVGNTVHLPAGRILGYHTSGDPNGIPVIYIHGNPDSGIQITGELETKIAKKLGVQWLGPDRPGIGLSTMYENQWVTDYPEDIRCLVNHLALKEYYILGTSGGTGFSLACARDPVPSQLKGVGICAGIGPVECGFDSMGETIKKAWDLWRDYPTEMQAYIEGEYVHSSQAADPTALRARIEADLKSFLTGDDLDHILQDGMLESAVQGYRQIYAQGAAAHAKGIEVNLKPWGFKMEDIKFPGIRFWYGSAEENTTPTMGRWMADRLPGAVYKEYPGKSHFTIWNEEILEEMLRDLIDGKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y889</accession>
    <name>A0A9W8Y889_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">WD40 repeat-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_005054</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y889"/>
    <dbReference type="OrthoDB" id="361494at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000109">
        <property type="term" value="C:nucleotide-excision repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042238">
        <property type="entry name" value="Rad28/ERCC8/Ckn1/ATCSA-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46202">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46202:SF1">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="52"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="195"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="262"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="358"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="404"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="361"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="413"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="423"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4370533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="458" mass="50551" checksum="49801E2BEA7BE374" modified="2023-11-08" version="1">MHTQHLLDRAYGLTSHHVLKRTTTSQLIYALQPAALRFSHHLPSADNPQEQVPAHVSGVNAITIDKFEGRYLLSGGADSSLAIWDLEAQLNATDGGETCLPLEKVAKTSEEHKLGITQVQFYPFDSLAFLTSSYDHTVKIYSSETLVPSASFDLDSVVYNIALSPIASHLLVACATQHPNVRLIDLRSGANTHSLAGHSGAILSTAWSPVREHILVSGATDGSVRFWDVRRSVGELGMLDLEDSIGVLGKPTSSFSRNARHGQAHRGPVNGLVWTEDGRHLVSCGHDQRIRVWNTDTGANTLANFGPMVKNSGLAPCIPVLPPADNFQPGADIMFYPNEHEILAYELFDGKLLRRLKRPQPRQQNHASNSAAAQNQRSAKDRVTSLTWRPHDVEMYSAHADGSIAAWKPRTEEDAELDEEEEQERKEKDEGRKRKRGVLDEIYQGLTKRPITFGNMGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y903</accession>
    <name>A0A9W8Y903_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_005023</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y903"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF187">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="364"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="440"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="1"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="474"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4370502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="501" mass="54580" checksum="AF6903DFA77D3316" modified="2023-11-08" version="1">MPLPRITADIGGAEKYVWIANSFVVAASVPQPLFGQLANIFGRQVPFIGSTVLFALGSGIAGGAYNVGMIIAGRTIQGVGAGGIYVLLDIVCCDLVPLRERGKYLGLMFSWSGVAAGMGPVVGGALADSNWRWIFYMNVPICGVALLAILVFMRMKKHTEPQKIDVLGNFIFIPSVLAVLYGLVMGGIEEPWNSWRIILPLVLGGIGWIAFHVQQHFVRYPSVPERLFKNRTSATAFALTFLSSVMVQASGYFLPVFFQAVLGTTVVRSGVNFLPFALGMLVFAVVAGLVMEKTGMYKPLHAASFAVAAVGFGLLTLLDESTVKWAIFQLIVGGGLGLTLSTLLPAIMAFLSESDVASSTATYSFIRTFGYVWGITIASTIFNGIFDHNLSRISSMELRQQLGNGQAYGFASRARFLRNTVSPNTWAEVIDVYQHSLRSIWFMCMGISIVCFCLVWVEKSIPLRDRLETQYGIEDQDDTKRDSSQMEMASGTELTETTHKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y967</accession>
    <name>A0A9W8Y967_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HORMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_004772</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y967"/>
    <dbReference type="OrthoDB" id="1928087at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051598">
        <property type="term" value="P:meiotic recombination checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007130">
        <property type="term" value="P:synaptonemal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003511">
        <property type="entry name" value="HORMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051294">
        <property type="entry name" value="HORMA_MeioticProgression"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48225">
        <property type="entry name" value="HORMA DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48225:SF7">
        <property type="entry name" value="MEIOSIS-SPECIFIC PROTEIN HOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02301">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="The spindle assembly checkpoint protein mad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50815">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0469">Meiosis</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HORMA" evidence="7">
        <location>
            <begin position="58"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="329"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="588"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="336"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="376"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="624"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4371553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="644" mass="72488" checksum="EDA7153771290CE4" modified="2023-11-08" version="1">MAGKTTGKSSKARTNQAKPAPMHTADAIAQVQGTQVLLQHPTPATTTTISTVQQVDVEQSVQIMQTMLNGCLSSILFQRSIFPPNCYESRYYVNSSSQWTYKDYLGAQQGVIKGDGAGGTILALKKGVSNRVDRFLSLMEMGIFDTLRRGFLRSLHLSIYESHPSDILESWTLTFHYVDVPAGGRVPSSFDLKERQGTTITLNQVKLSLNDFIRKLTGLCMTLPALPEDKKIALEIGYTNNRPQDYFALGFPHPVYDLVRFPSTDDWEKVTSDVAILHTGRHAASLKVSHLSRQNDSITNALPSELKCTEETRKLDDIMVDLAERAQAVSKPRIKSQVSKVVDPSSSVPADNSDQPTGTAPTSPVGLDVRPPSVSDSRRPSVERQERQRIEEMAKIDQVDKERSVTRLPRKEIFQTRTANEHVDQITIRCQCGHTGEEGHMVLCEFCEHYQHLHCYGYHGKDDIRLPNIHVCYQCLLDETDEQLRQLREHAMHRRALWFLQNNASFTSLRSFARSLSYTDRETERLLKKFRTLGLLTSVGSFKRGLGPIELSPSESAQTLMDQMYFDPAFGITHLLEDLALRDDEARRPAGHVESNKRPRQDADEELPPPKRPLPYASGATFIDGKEVNTPKSDKTHQLSLRRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9E1</accession>
    <name>A0A9W8Y9E1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004383</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9E1"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="244" mass="25256" checksum="506A0DA4474E4A42" modified="2023-11-08" version="1">MASNEPVITKTRRRIKDPYAIDSSDDDLDDLDEDDEVEDQLTALPYSSTSTSQPQPPLAPVVAQKPQRQERQESLMDFLNGMDPPSTSKPQPFMLSQETIAAARARAAASNSSLSSANTAVRNGNGIGNGNGNGGVSSPYGAVSSTSLGGSDGASASVGGAATRNYNKPRLAARAPAIAGSDGGRSGGGAGGGSKTATSDLADFLRNSGPPEPPVRSERKEEDGGGKKGSRKFWRSRKTYGDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9G4</accession>
    <name>A0A9W8Y9G4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein YIF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hrf1</name>
        <name evidence="10" type="ORF">N0V83_006333</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Has a role in transport between endoplasmic reticulum and Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Golgi apparatus membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the YIF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9G4"/>
    <dbReference type="OrthoDB" id="337750at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005578">
        <property type="entry name" value="Yif1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083">
        <property type="entry name" value="YIP1 INTERACTING FACTOR HOMOLOG YIF1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083:SF0">
        <property type="entry name" value="YIP1D-INTERACTING FACTOR 1, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03878">
        <property type="entry name" value="YIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="140"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="198"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="262"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4369248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="281" mass="31289" checksum="A8F05343616EA71F" modified="2023-11-08" version="1">MPQQQGGYNMHPSFGGFINDPTAQLGFQMGKSAVDAGQQYMEQNLGRYVSVSALKHYFNVTNSYVLSKLRIILFPWRVERWSRQLKNPDAASGASSLYLPPRDDTNSPDMYIPTMALVTYILLSTLLAGLRGAFHPELLGYTATLAISVTLLEILIIRTGTFLLAISNSSQLMDLVAYSGYKFVHVIVSLLLTHLSSWLGFGGATVSWIIFLYCFNANAFFLLRSLRYVLLPDQGGQGMGVGGNPAAGYTVARSQKNRRTQFLFVYSYVVQFGFMLWLSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9I5</accession>
    <name>A0A9W8Y9I5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Isochorismatase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_005770</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4370006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9I5"/>
    <dbReference type="OrthoDB" id="167809at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00431">
        <property type="entry name" value="cysteine_hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050272">
        <property type="entry name" value="Isochorismatase-like_hydrls"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540:SF9">
        <property type="entry name" value="FAMILY HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G08700)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540">
        <property type="entry name" value="PEROXYUREIDOACRYLATE/UREIDOACRYLATE AMIDOHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="4">
        <location>
            <begin position="68"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="37"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4370006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="301" mass="32069" checksum="D471C93C1691E785" modified="2023-11-08" version="1">MAILGDKNESRGDAPVVAGHDQSNSWSFTPTTGWDLTRTTNPSTTSSSSSRITLETTTTPITIDPSKSALVIIDMQNFFLSSALGNAKGEGHAAEAALLTSAIPAATQAGIQVIHITWGISDDELAVLPPILDRIFGFDIDAAKPNANDRERKMPRGIGHPMGTVTLADGAQVDAGRLLMRDQWNTELSDALLQDFEASQNSTKPHVRFHKNRISGLWAGGSTPFREYLKENGITTLLFSGVNTDQCVLGTLQDASNQGFDAVLLRDGCGTTSPGFASEAVGFNCRRIWGFVSSCGALVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9N6</accession>
    <name>A0A9W8Y9N6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">60S ribosomal protein L7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RPL7_2</name>
        <name evidence="7" type="ORF">N0V83_004549</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9N6"/>
    <dbReference type="OrthoDB" id="28644at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.15.30:FF:000001">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000002">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.15.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018038">
        <property type="entry name" value="Ribosomal_uL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005998">
        <property type="entry name" value="Ribosomal_uL30_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012988">
        <property type="entry name" value="Ribosomal_uL30_N_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01310">
        <property type="entry name" value="uL30_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08079">
        <property type="entry name" value="Ribosomal_L30_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00634">
        <property type="entry name" value="RIBOSOMAL_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30 N-terminal eukaryotes" evidence="6">
        <location>
            <begin position="14"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="5">
        <location>
            <begin position="90"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4371332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="249" mass="28747" checksum="D813D02058ECA52C" modified="2023-11-08" version="1">MARSSVPTQNDIAVPESVLKKQKAHQKTTDSQAAELKKKREANKQKRETLFKRAEKYNQEYVKAQRDVIEAKRAAKKDNALFVPAENRLVFVVRIKGINKIDPKKRKTLQLLRLLQINNGVFIRLTKATLEMLKIVEPFVAYGYPNLKTVRELIYKRGYAKVNKQRIPITTNDIIEGSLEKFGITSVEDLIHEIYTVGPNFKQASNFLWPFKLSSPTGGFRTRKFKHFIEGGDLGNREEFINQLVRQMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9R0</accession>
    <name>A0A9W8Y9R0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PIPK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_006290</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9R0"/>
    <dbReference type="OrthoDB" id="70770at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF126">
        <property type="entry name" value="PIPK DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="4">
        <location>
            <begin position="1"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="222"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4369205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="379" mass="43626" checksum="FC00143DC60491F0" modified="2023-11-08" version="1">MGYRDRLISTSILRAIFWTKPSQDDPEARPGILARILNFFSFFNLTLARYRAALFRKIRNETWGIEEEEYAESFRSPSKNKRTDLVAVGDLGYSGSTFFTTPNSKYLIKSLPRQFEQSFFRKRLLDPYVEHMELNQDSLLVRITDLLYCRTYTIGAITGAAPSHHMVMENILYGKSSCSKHHQQQWETYDLKPNDYFYPERDVAGGMLAPESVKERLIDEFNDKVRVTIDQKEELISQLSRDSKILQESNAVDYSLFLVRYPADLVSSDSDKIPTPPGRGSSWRNGVVSRDGRWVYRAIILDFFWAKDALQAQTLTGLVKLFNFVKWGKDHGPMSITTTSDEYRQRFLGMVENMVEVPDSAGRPSGEVRSPTPQRFGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA77</accession>
    <name>A0A9W8YA77_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004681</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA77"/>
    <dbReference type="OrthoDB" id="3644322at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="561" mass="62020" checksum="DAC4D7D8C3E0917A" modified="2023-11-08" version="1">MSYVAPDFLDQDTQVQEERGHDDAPSIEDCVHTFLNNTRHRFGEHSVEVNTINAILNSFRNGEITKRDALSSITLTLRDQDDIKQDLMNVLYHKDAKWGADDFDFDQLVAQSCPQFLQPAHEPQMRLPPISSLYPNRLAVQSINPAVLNVYGGLATLANDVYSGPIFAQHGNPNWTAPAQVPSSPQWQMPTSFHSLNQPAMTQASEFSHYKNANEADMSPAHEHFSGVAHPRRIQGDWENIDHSDDYATHIPIKKEVSADASPSVLGSPFAMPLGRPAVDMPPPPLKRSHQSVSSTPGTPTTPAATLHQPKKQPSSTVKVAQSQREAPSFIHSICGKGFSSRSKVKKHHWGAKNDDLNTTTGCCKFHIFRPASINFVLISSFQIQGVKHSKPNVSWDEHPSCKESTVSQRAVKKTNMQSTPRDHLSEQKAPVVPAMIPSYNTIPGFPILQDLPQTVAQALTLSNPTQHTFEHIGSYHSHRLPTSSNSFDSLLTAVNVASRIDAPKPQGRNDSVVSHLDAQAAAAERDVRYTSTCIDDGSLVRTSLMPEERSRFLLRSREPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAD4</accession>
    <name>A0A9W8YAD4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SMP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_003928</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LEA type SMP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAD4"/>
    <dbReference type="OrthoDB" id="2799468at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042971">
        <property type="entry name" value="LEA_SMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007011">
        <property type="entry name" value="LEA_SMP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31174:SF7">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT PROTEIN 31-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31174">
        <property type="entry name" value="SEED MATURATION FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04927">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SMP" evidence="4">
        <location>
            <begin position="333"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="280"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4372155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="382" mass="41800" checksum="A0D29E4ED94BB8DB" modified="2023-11-08" version="1">MAEMINDKVSSSETPKLAREEIFQKDMENILVKVKNRPESIIAEDVRRLSGGVDTLDEQSGRIISLLGTLALANGPLNEQDTNVDQTPHASLLTAVKNLNAAVDTNPDHVTTEVLRSAQSIVNKMQKAIGNASVPHPDLEAELQEETTKIEPKVARGTVTKAEADHLHSLEARAHGHTDKGGIAAIAQSVAAKRERRISLSHTISLDSGRSRANSKIVTATPQEQSHHDKEANLHEAEITNKPKLEQGTVTQGDADFLHSREMRAHGHIEKGGLAATAQSIVSKKRQESLSDRSNVSPRASEADYERRKEQSHHDKENNLKIAELTIEPKIEEENINKEDAAYVQSLENRAHGHIEKGGVAAEALSKAQRRENSQSVAESSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAT3</accession>
    <name>A0A9W8YAT3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Endoplasmic oxidoreductin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ERO1</name>
        <name evidence="20" type="ORF">N0V83_004267</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">May function both as a monomer and a homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EROs family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAT3"/>
    <dbReference type="OrthoDB" id="269384at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016972">
        <property type="term" value="F:thiol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007266">
        <property type="entry name" value="Ero1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037192">
        <property type="entry name" value="ERO1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613:SF0">
        <property type="entry name" value="ERO1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613">
        <property type="entry name" value="ERO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04137">
        <property type="entry name" value="ERO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017205">
        <property type="entry name" value="ERO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110019">
        <property type="entry name" value="ERO1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 17" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="15"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="411"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="417"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="16">
        <location>
            <position position="313"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="316"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="18">
        <location>
            <begin position="313"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ4372493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="474" mass="54416" checksum="1A7A868FF574E964" modified="2023-11-08" version="1">MAYIPLVWRAKELGKLEGPTAQHPGKKQQREERKRPLHGSLGEDVDESCVVEYDDECDQRDYCVPEDESATAKGDYVSLVENPERFTGYAGVGAQQVWEAIYRENCFSKPPKVAPSTGKSAPLGGFGNQQQMFAANQLRNVIKEQGMQQKVQSAIAHGTPVERLDPVEFDDTCLEKRVFHRVISGMHASISTHLCWDYLNQTTGRWGPNLTCYEQRLHNFPERISNIYFNYALVMRAVGKIKQHIQDYSFCSEDPEQDRFTKDSVLRLASALPSGPEIFDETIMFQDPDTIALKEDFRNRFRNVSRIMDCVGCDKCRLWGKLQTNGYGTALKVLFEFDENDSSKDPPLRRTELVALINTMDRLSHSLSAIKEFRRMVEEREGVSRAIVPETEKEVLKTRRALEGFDGEATAKSEIAADTDTDTDDDVDDGMPDFRRDWDARNMTLSEEFWAEFNLVRRTFWYVLWQWTQLPGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAZ0</accession>
    <name>A0A9W8YAZ0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_004241</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5563754at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000203">
        <property type="entry name" value="PH domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058155">
        <property type="entry name" value="Skg3/CAF120-like_PH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25381">
        <property type="entry name" value="PH_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="119"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="17"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="474"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="712"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="768"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="851"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="991"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1271"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1164"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="486"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="498"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="516"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="721"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="821"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="879"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1062"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1073"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1281"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1309"/>
            <end position="1325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4372467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1325" mass="146556" checksum="EF1C587D84B76DEB" modified="2023-11-08" version="1">MLSARSSTVLSFMSQWGGNATLQKNPPSAGTSPGPASPAPASPSPNPPTSSALSQSATALDHYNHERKASREYARPDSRASSRPMSMIQTYQPPVMEVGHDTLPELQRIFTFLNSHSNKLYQEGYFLKFHDTDTRGRPAPDRKWQEYFVQLVGTILSLWDASELDRAGGDAEVLPTFINLTDAAIHMIPSMTLKDGKKLDNILSVSTAASNKYLFHFDSYHALTQWTAGIRLAMYEHTTLQEAYTGALIAGKGKTLNNIRIILDRQCAQYDDWVRVRFGAGTPWRRCWCVVSPPDQKEYAKLQKSQKKTSLYDRSPVQLKGDIKFYDTKKITKKTKPIATVKDAYSCFAIYPQSKPLIDQSTLVKIEGKITVHSNPESTTEGFVFVMPESRPAVSGFEIMLQYLFPVFDTFCLYGRPKKLIADVLDSRGLMFAMPSDRRYGYLEMWDVVGLINTEGSATWSERQWRKQLKDLTSTRMSAAGPETESVYSRASRRNTVSRSGLSSRGNLRFEDPVSVRSNPTTRQPSPTRQRQASFELQEPRRAGTAPAAGPFAPPRHQRSVSEQVNGYTNHNKYQTMPSRLAQGQLADDYDRAPTPPTHGVYNAAPYNGAVQHHEVYDSDESPIHDPQNLPQLPTDLSAPPQGPVDLPPTFAHAANQQPPVRPNLPPMMKPNPAVDSATLHQMADASNTPLPAGVAAAGAAAAWRSSESLHSRRSGDYNAYPQNGQQMYLNANTRPPPSNHNSGNRLSTIPASPYVEHAEFVQASAGYQPTAPPVPEHTELPQASPSQELPYRPRPGSGGIQRKPVPGRSPLPSRDEDTRSTTSSSLGSLRNIIVDPDALDALNHTETTLFRQQSTSSSRYDDDAASTSTPDYASVASEEIRPRKLPERREDRPRSGVLRTVGDPNYKSKFDVGPDGCLTVPDGAQGSANVPSFDFGPTYALGFDDKRPGTSGTMTQMLHDGTLSKSRENLATTPNEQHQQLYVSGRSTPNAAMHLRSGSGSPQALDSRSVPWQPGMASPQQPDRQTLDPEEWVMHRASQQPQTMLHGHARTKSKTPPPFGRTTSGDWSQLQRSPDGSSGLSRPPSRPLSRPQSRGALLDQRPMSLSAREQEQVARMTNTPLVDLSHNSKKLQKQPPQSLTGYIDFREKEKAAAKAARQGHSSVMQAEIERRTLQAQQRQMMEAQQRQRQFMEMQQQQMHHQMAYAQSSYAPSMMGTPSGMGTPSVMGMPIGPGTPQGMAGMAYSTPNQMSQMYQQQQQQQYFPQQAATPHMHIPGAWGSPTPQTPQGQYFPQQQQPQPTQPYGASFDQAQAAARYAHQQGQYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB87</accession>
    <name>A0A9W8YB87_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_003496</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB87"/>
    <dbReference type="OrthoDB" id="1933717at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42901">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42901:SF1">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4373203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="297" mass="32451" checksum="32EDAD7E051E49EA" modified="2023-11-08" version="1">MGANFTSEHHDTYPYIDPTNADLSGKYVLITGASKGVGKSAALSYARAGASGIALGARSSLSSIVLEVQEAAKSAGRPEPKVVPLHLDVTDRKSVEAAAQAVSEEFDGRLDVLINNAGYLSKWGGIPDTDPEDWWRDWEVNVKGVYLVTRAFWPLLLKSPPSTRIIINVTSMGALMNGRHGSAYCSAKLASVRFTEYIHQDHGEGSADGMLAIAFNPGQLKTDLALGLPEYLHGYLMDGPELSGDTLVWLGKERREWLSGRYVSANWDMEELSAKREEIVRADLLKVRMAVNEFPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBK2</accession>
    <name>A0A9W8YBK2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_003380</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBK2"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000013">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000018">
        <property type="entry name" value="MFS transporter permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF3">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="437"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="48"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4373089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="488" mass="53918" checksum="3794F3CC23FEC97F" modified="2023-11-08" version="1">MNNDDHEKDVTIQNEKYSQPVNENNGVDYEAFDQIEEKRLIRKVDWRLLPILGALYSIALIDRTNISNARVAGMGEDLGLQIGNRYTIVLVVFFPTYALLELPSNIILRKVGSANWLAFIALSWGAVMIGQGFVKSWITLTICRVLLGAFEAGFFPGCVYLITCWYCRYETQKRLGGFYLFSVGIGGLANILAYGLMQMEGLAGLRGWQYIFVIEGIMTCVVAIAAWFIILDFPDKAEHKGFLTRREAAVILQRIENDRGDSVADPLTWSKFVGHLADLKLWAYATLFMSTTMPAYAFSYFLPVILVGMGYSAGQANALAAPPAITAMFTAFGFAWLGDKYRLRAPVIAAQSLLAIVGLMIVAYAENNGARYFGTFLGICGCQGNVPAILAYQSNNIRGQSKRSVGSALQIGFGAIGGILASTTFRQVDAPRYITGLWVTAGLQFYILIVLCFTSAYFWTKNKKVDRQIANGEPVEPNEGQVGFKYTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBL1</accession>
    <name>A0A9W8YBL1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_004288</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the gamma-BBH/TMLD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBL1"/>
    <dbReference type="OrthoDB" id="406634at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045329">
        <property type="term" value="P:carnitine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000058">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2020.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050411">
        <property type="entry name" value="AlphaKG_dependent_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038492">
        <property type="entry name" value="GBBH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696">
        <property type="entry name" value="GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696:SF25">
        <property type="entry name" value="OXIDOREDUCTASE AIM17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="7">
        <location>
            <begin position="220"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4372514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="474" mass="54454" checksum="FE879F71DEFD855B" modified="2023-11-08" version="1">MSFNVSTRLPLRTISSKSTRHCCRATSAAQRRQFFFKSFWSRSPSAAEHAEWSRATSGPKRPTKLEVKQQQEQELRQAKEQESLLHESAHIGQVDGESAKCIAVNIHGAPRIFDTAFLRDSCTCPQCRDPDSKQKLFQTSDIPADLEGSYKTVVDEEKGPSIELEWKTDVKGYGPEHRTRHSIDWLARALNTEVELRGGVRHDDRVLWNREKITKDNKWVDYNEYMSQDETLFDALTHLNKYGLLFIKNVPESEESVVDLAGRIGTLKDTFYGRTWDVRSKPKAENIAYTPQFLGLHMDLLYTSNPPHLQLLHSLRARTPGGESFFSDSYHAAHQLQRHSASHFRTLCNFPRPDPTNLAIKRVNWSPPFQGPFEARIGSNHQTSLRSFIAASHAYEKLLSAEENLFEYRLNEGECVIFDNRRVLHARKAFDATKGERWLKGAYVDDDVFFSKLRVLEERFEGRWCAEGVVRHGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC36</accession>
    <name>A0A9W8YC36_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003626</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC36"/>
    <dbReference type="OrthoDB" id="2142040at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34706">
        <property type="entry name" value="SLR1338 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34706:SF1">
        <property type="entry name" value="VWFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="46"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="259" mass="28467" checksum="FA5DA940E1487306" modified="2023-11-08" version="1">MNPFASSSEAPPAYTPGPAAQTNVDASRSNQTLTDTDPYAFLKTFDTLFLIDDSGSMAGRSWRETADAIKTIAPICTERDADGIDIYFLNHPDSSLYKNITTAGTVVEIFETVRPSGATPTGQRLNKILRPYLQRYERAPETTKPINIIVITDGEPSDDVESPIIQTAKKLDKLDAPAWQVGIQFFQVGKERGAREHLKQLDDGLKELAGDDDLRDIVDTVPFTGEGHAPLSAEGIIKVVLGSVNRRLDRNSKELHRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCB8</accession>
    <name>A0A9W8YCB8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_004627</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCB8"/>
    <dbReference type="OrthoDB" id="2962993at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF54">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="381"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="418"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="443"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4371410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="55734" checksum="F3DD83A94463AAF4" modified="2023-11-08" version="1">MADIAKMQSNSLSEEKTNPETSVTAIAEYDEYVGLCEVMTEDKMKKLVRKIDIRVLPQLIILYLMSYIDRTNVGNAKLFGALADMGLSGQDWNTALSVFFITYALGAVPSNIMLQKFGPRIWLPTIMLCVSIILICCSMQSTMAGWTTFRVLLGLVEAGVFPGCSAVLTTWYAAGLNEVSMRKCADIHCCRYSPHEVHTRMTIFYMGASAAGAFSGLLAYAIGQLDGTWGYKGWRWIFCLEGIFTALLSIAAFWIIHDTPSKVSWLTAEEKRFVVLRHKFSAGGESGVAEKEEFSLAAVRQVFEKSSLIMSSIKNLGYSAATAQAMTAPPYIFASLVTVFSGWAADRYKQRMLSVLLPNLLAMIGFIIIIVSVRYKDLPGVTLFGIFFAIGGLYPISPAVTAWTALNLAGTMKRSVGIGAMIAFSQLGGIVGSNIYISTQSPTYPVGFGVSISMLGLFGIVWPACYSFMLKRINANRAKISAEEVRAKYTEKELAEMGDESPLFRYST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD07</accession>
    <name>A0A9W8YD07_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">TAT-binding protein-like protein 7, AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">YTA7</name>
        <name evidence="13" type="ORF">N0V83_002870</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5421at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140674">
        <property type="term" value="F:ATP-dependent histone chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05491">
        <property type="entry name" value="Bromo_TBP7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001218">
        <property type="entry name" value="AAA family ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000016">
        <property type="entry name" value="ATPase family AAA domain-containing protein 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000061">
        <property type="entry name" value="ATPase family, AAA domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045199">
        <property type="entry name" value="ATAD2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069:SF0">
        <property type="entry name" value="TAT-BINDING HOMOLOG 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="12">
        <location>
            <begin position="1179"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="189"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="468"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1263"/>
            <end position="1457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1500"/>
            <end position="1525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1625"/>
            <end position="1666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="242"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="257"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="296"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="313"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="340"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="351"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="369"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="513"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1263"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1345"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1355"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1376"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1393"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1501"/>
            <end position="1516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1630"/>
            <end position="1643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4374129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1666" mass="184702" checksum="694CAD60D56155E2" modified="2023-11-08" version="1">MSSRTKRNVAHHLNVVAIETPRDPKKRPSKRQRRGYGGSDIDEDDQIESDDSFTERSESEEPEINPATGRSVRRATKKQFTYEESEVEEDEIKDTSSDDEDEVPTTTSRRRAQPSIEEVEKPSLIVKLKMPENAPGRNLRTRTGSKSIARGKTPEVAGARRSSRLSHDVEAPIVQLSDSGRHVDIVRQGTRSPEPVIARATRGGKGPRVQHPSAIMEASQETSMIRDESPGPLDALLAGAETQVQASKDSSPIQEAQQEEEDDEDEGMEGVIQESQHEGAAEDESEEEGPVTRGGRNLRARATSTKRKRGADESSDFEPAAEDEKEEEMSESDHEKGKQSASESASGSGRRSGRLRGRTRQSQRSRRNSSSDESRLDPNELQDELEELGTNKRRRLSRRVPDNDLSYDSAPRRRARAAQPDYRVVRPELNAVFEADDDAPAGEKSRSRGGRSAYRSLWANRGPFGGGVEAGMGAGAGQDSDSSDDDNQKMPKPLGGMVGMTPTTATAPAFGLPSQTHNADPQQASGGGPANLGKVKDRKALADADPLGVDPNVNFDGVGGLDDHINKLKEMVMLPLLYPEVFQRFKITPPRGVLFHGPPGTGKTLLARALASSVSGHGQKVTFYMRKGADALSKWVGEAERQLRLLFEEARKTQPSIIFFDEIDGLAPVRSSKQEQIHASIVATLLALMDGMDGRGQVIVIGATNRPDSVDPALRRPGRFDREFYFPLPNVAGRRAIIDIHTQKWEPPLKPDMKDQLAELTKGYGGADIRALCTEAALNAVQGTFPQIYTSERKLLIDPSTIKILAKDFMISVNKMVPSSQRTVTASAAPLAKNIEPLLRKPLEEILKRIDELIPRRKKLTALEEAEYDDREDEKGFEREATMRNFESSRVFRPRLLITGLQGMGQQYLGAALLNKIEGLHVQSFDLPTILEDPARTPEAAITQLFTEVRRHKPSVIYIPAVDVWYATLPGPAIKTFKLLLRSVGANEPIMLLGVMELAHEKEKPDRQMMMDLFSFSQSNQFQLARPDQEGRSEFFNSISHYIRMSPADFPDPDNRKKRVLPELEFAPITAPVVDPKELANREKLQRKQDRMTLQRLKILIQPVMDQLKRAYRKFFKPILEEGWYAYLLDEQDPEHFRTDLPPEQQQEQGVVRPWEFSKDAKGVDVLLHVDSGNKYYNLDLGNIEKRLSNGYYKRPKDFLFDIKTLVKDAKTYGDAERVLKGNEMAANVEVDMETIGQQNPGLLAECEAVYIRELERERKAQEKRDKALAEGKDVPADVARVPPEQSLGTTEASGPIMLGEPVPGHATMPPVTPVRLAPTGPLSNGTSGEEGAHSHLNGSTVPSRTDEDTHMTDSQDLNVNTQNESHFQTPSRIDTQATQTQKSQTSGRTFMAPNSNPNDYHNSASTTTSGQKTSNRSSDNKFGTQSSNGAAPAGIPNFSEMMPEAGGSQLPDTQGSRNFIRRQSPQLFRRFPPQFPYPFPPHQQITTTNHVPNAEVHYISSQPSNSQPSQSSQMPAPAPPRHTTNLADILNEEEPKPQLILDETMLTELHRNLVSSTSGCSLEQLEQINASLMDAIWKHRADYNRNVVLQQVKEAFNVIIEDIQIMQKILKSSQEDEELETQGARYHGYGIGGSGGGGGGMQLGLPPPSQDDWLTGAQGTQDMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD55</accession>
    <name>A0A9W8YD55_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_002650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD55"/>
    <dbReference type="OrthoDB" id="3768069at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="231" mass="25204" checksum="200CE3BFBCD5118D" modified="2023-11-08" version="1">MDSTISQHQAPQNHHSAAESEVSLASNSDMLFSNIGLVLSLVGFTATSVYAAPMEYRLEDIQCRCLSFSTSAKPTLCTYLESHGLDWDTAYSLASDNELKIQFASQSTISKVLDIRKPLPSSVLRTLSHGEAAPLDVSSSLLQSENKIVCGLGDEVKHMGSPDMDLSSECHYVGAVVAALMLFLIAYLVGEHIWTKYFRSQEGNIKLEGDEKTLTATLHHEDCVDAPTNFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDF2</accession>
    <name>A0A9W8YDF2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Condensation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_001955</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Mycotoxin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NRP synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDF2"/>
    <dbReference type="OrthoDB" id="416786at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19542">
        <property type="entry name" value="CT_NRPS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19534">
        <property type="entry name" value="E_NRPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.30:FF:000002">
        <property type="entry name" value="Nonribosomal peptide synthase Pes1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.30">
        <property type="entry name" value="Nonribosomal peptide synthetase, condensation domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001242">
        <property type="entry name" value="Condensation_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45398">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45398:SF1">
        <property type="entry name" value="ENZYME, PUTATIVE (JCVI)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00668">
        <property type="entry name" value="Condensation"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Condensation" evidence="5">
        <location>
            <begin position="117"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Condensation" evidence="5">
        <location>
            <begin position="488"/>
            <end position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4374877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="856" mass="96687" checksum="11795BD3A4DC3A20" modified="2023-11-08" version="1">MQVVGQCRKEGMSLGVQEVLRSRSIIQLATAVKELESSSYDHEEYFDEDFDLSPIQSLYFQRPNTHGHFNQSFYLRVTRRTTVAQFQSAVEQLVARHSMLRARFSFSEERGWQQRLTNSQQCFDHTNGPLFAADFFDFGNEQYAFLTGHHLVVDLVTWRLLLEELEEILKGGQLLAPALPFQKWAELQSEHAETLELDEVLPPVNVPPMDFSYWGIQHQDNTYGNAGHASFELDTTLSSAFLTSCHTAYKTEPVEVLLASLIQSWSQVFTDRPVPAIFNEGHGREPWKSDIDITRTVGWFTALSPILVAPSQNPTDTVRKTKDFKRRIPGNGRPYFARRCLTEDGREHFKTHWPMEILFNYLGQYQQLERPDALLQPLGTMAGETGKAGGTSDFGHDTCRWGLFEISAFVFKGHLKVAFTFNRHMQHQDLIQQWISECQNTISKTIRELSLLDPKPTPSDFTLLSVTDESFYSMLDRLAGMGIKDSDVEDVYPCSSLQEGLLLSQTKDAGFYAAATLHEVKAPNAHLKWNAVADAWRQVVKRHQALRTVFLENVGAKEGLYNSVVLKDIGANIVHIECQNGSEAIGLIEEQRSISYNHGRCPNHRFTVCTTLDGRMFCSLEISHAIMDGHSMSMLVFDLEKACEGQLRLDGPLYSDYISWLLKQPQESSLEFWKSYLGGSEVCSFPVLDDGKTTEKELVSIRMDPTSISLPDLQGFCNTNGITLSNVFHTAWALTLSCYVGSNDVTFGYLTSARDSEEVHGVEDMCGPIINTLVCRVNLSDGSRCLLDVLQDVQRDYMEAIPHRHIALADVQHVLDLSGASLFNTALSYRRLPQEQPTDGNRLQIVEAKPIYDPTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDQ8</accession>
    <name>A0A9W8YDQ8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Interferon-related developmental regulator N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_003259</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IFRD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDQ8"/>
    <dbReference type="OrthoDB" id="18978at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039777">
        <property type="entry name" value="IFRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007701">
        <property type="entry name" value="Interferon-rel_develop_reg_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12354">
        <property type="entry name" value="INTERFERON-RELATED DEVELOPMENTAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12354:SF1">
        <property type="entry name" value="INTERFERON-RELATED DEVELOPMENTAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05004">
        <property type="entry name" value="IFRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Interferon-related developmental regulator N-terminal" evidence="3">
        <location>
            <begin position="72"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="299"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4372968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="448" mass="49875" checksum="80C8634AF70D8EF0" modified="2023-11-08" version="1">MRDLRRRALESHKTVSRKARSTVASAASSKANSTANSPAPSRAASRSRQVSEDEDDYHSDGTAWSTNSIDEVINGEDVDVPEDAWKAELNVRVEQIINLKRSSTEGRTESLIAYAHVLMARYGKHEIEGHVHDLVTSMIKSIRAETTEREAVVALKALAVTVVTLDSDTIYDDVADQLKRSIQSSESVEIQVNAIHALGIAAFFGGAGDDEILDIMTLFVEIIESDGASVNAQDEGNVVIAALENWGLLATEIEDLEEETETAIEAFVEQLESADAGVQIAAGENIALLYEKSFTIQEEDEDLSDAEDPDDPEHTKDGQKMVKRYQVYRRQDQLLHTLEELASVSTRRISKKDRKTLHSSFADIRNSVEKPTRGPSYSTAIDQESGKAYGAGRMKIRINRNCELRIDKWWKLQRLNALRRTLQGGFTYHYEQNELVSSALPFSMSSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDZ2</accession>
    <name>A0A9W8YDZ2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">TOM (Translocase of outer membrane) complex component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TOM70</name>
        <name evidence="13" type="ORF">N0V83_003394</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Tom70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDZ2"/>
    <dbReference type="OrthoDB" id="2942533at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005687">
        <property type="entry name" value="Tom70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00990">
        <property type="entry name" value="3a0801s09"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="348"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="416"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="484"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="559"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="66"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4373103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="643" mass="71434" checksum="C93BCE4D831B55C6" modified="2023-11-08" version="1">MAPTNAPLPNTPGPFPVQIPVDQGTSSLWDRLSNWASENKGVVYTIAGITLVVGAGGAVYYFSNTDSRTDESATESSANKRKRQRERKRAKERAEKESATEKSTTETATEPKKASVAAASEDELPDVDENSVGALSEEERKDYAAKLKAAGNKAYGSKDYNRAIDLYAKAILCKQDPVFYSNRAACYNAMSDWDKVIEDTTAAINLDNEYVKALNRRANAYEQVERNSEALLDYTASCIIDGFRNESSAQSVERLLKKVAETKGKAILAGKEKKLPSPTFVTNYLQSFRPKPAPAGLEDDADLDEESGKGQLRKGLKAMATKTGEGYTEAATAFDRALELGDLGDHEAFAYNMRGTFSYLKGDNEDALKDMDKSIELQPTLTQSFIKRASMHLELANPDAAEADFAAALEQNSSDPDIYYHRAQLHFIKSEFGEAAKDYQKSIDLDKDFIFSHIQLGVTQYKMGSIASSMATFRRCMKNFTQVPDVYNYYGELLLDQQKYQEAIEKFDTAVEMEKTSKPLGMNVLPLINKALALFQWKNDFSEAEKLCEKALIIDPECDIAVATMAQLLLQQGKVTEALKYFERAAELSRTEGEIVNALSYAEATRTQLEVRRNPNIKYPHTVVINGVPFSQSNLTTILTRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YE51</accession>
    <name>A0A9W8YE51_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NTF2-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_002248</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YE51"/>
    <dbReference type="OrthoDB" id="5440at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030638">
        <property type="term" value="P:polyketide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009959">
        <property type="entry name" value="Cyclase_SnoaL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38436:SF3">
        <property type="entry name" value="CARBOXYMETHYLENEBUTENOLIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38436">
        <property type="entry name" value="POLYKETIDE CYCLASE SNOAL-LIKE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4375164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="385" mass="42875" checksum="24EA1CD599A812BF" modified="2023-11-08" version="1">MSITSFEQSGSSFLGFNSSPPRVYITTASPAPEKALLAWKEEGFDAQHLPYDPALQKTYIHTLKTLGESLKLGEHYAIIAYGEAASVVLKTAQKPLPKCCAIIAFSPTLLPGPKTKYPNSHQLQIHVAGLSQASARPEMCEWKLYRYEKCATGFADPTSKAYSEVEANLSWSRALASVRKGFNKNVDLEPVVQNAWNVKYEDEVPERASMGLVQNMTQNSPHVTIFPTLEGGVGRKKLEEFYREFFVPSLVEDFEIRLVSRTIGVDRVVDEMVVSFTHSDEVDWILPGVQPTDKFVEIPMVSIIAIRGGKLVSEHMYWDQASVLMQVGLLDPKLVPKKFKNEGLKRLPVVGVEAAKQLVEPQQQRYNKLLQEHGLLDGLNGVNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFM6</accession>
    <name>A0A9W8YFM6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Golgi mannosyltransferase complex subunit</fullName>
            <ecNumber evidence="10">2.4.1.232</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MNN9</name>
        <name evidence="10" type="ORF">N0V83_001468</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ANP1/MMN9/VAN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.232" evidence="10"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFM6"/>
    <dbReference type="OrthoDB" id="2405412at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033164">
        <property type="term" value="F:initiation-specific glycolipid 1,6-alpha-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000017">
        <property type="entry name" value="Mannan polymerase II complex ANP1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052086">
        <property type="entry name" value="Mannan_Polymerase_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083:SF6">
        <property type="entry name" value="MANNAN POLYMERASE COMPLEXES SUBUNIT MNN9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083">
        <property type="entry name" value="MANNAN POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03452">
        <property type="entry name" value="Anp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4376187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="368" mass="41414" checksum="6C3D363CFF17E413" modified="2023-11-08" version="1">MSRSFRRSNPITIVLAGILCIGFIFFFFSGSDAPIVGKSRGGGETASNPLSPPSLPFRKSKANAPQAAPPVVHYYMNNLTNTRTPIENEETVLILTPLARFYQEYWDNLAKLEYPHSLISLGFIIPKTKEGNAAYAALQAQVTKTQSGPKNKRFAAVTILRQDTESPLQSQSEAERHKMENQKARRAAMAKARNSLLFTTMGPTTSWVLWMDSDIVETPATLIQDLAGYDVPIIVPNCFQRYYNEDKKAMDIREYDYNNWIDSPTARDMGSKMGKDDILLEGYAEMPTYRSLMAKMYDRSADPGTKIKLDGVGGATLLVKAEVHRDGAMFPPFPFYHLIETEGFAKMANRLNWESFGLPNYLVYHYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFX8</accession>
    <name>A0A9W8YFX8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha-ketoglutarate-dependent dioxygenase AlkB-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001866</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFX8"/>
    <dbReference type="OrthoDB" id="2163491at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035516">
        <property type="term" value="F:broad specificity oxidative DNA demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051747">
        <property type="term" value="F:cytosine C-5 DNA demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032852">
        <property type="entry name" value="ALKBH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31573">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31573:SF4">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-ketoglutarate-dependent dioxygenase AlkB-like" evidence="3">
        <location>
            <begin position="748"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="75"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="923"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="170"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="770"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="779"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="898"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR632852-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4374790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="943" mass="104357" checksum="849118EDAFBC89D4" modified="2023-11-08" version="1">MTGLKRKIDERASNRAEKTSTEEPRPSNDSNEYELKLDMNNYGTAACLRPRRNTRPPASLFAAGFAAVLPTFATKVSRQKGKATSKAKQKPVPKKAKRAQETTFETSTGRKTQPEPGPDKDDHVEIPAVEENLSNVPQDNTWDPAVVQGTQSGDAHKSQPEPGPDGASRDASQTDVALTQVGTTPPPEPQVPGNSPSSPTHDGPQKAFETPQKVSSQDTQAFFAPLTPESMPRPVRAKPLLPTPIGASQIAAETDTETTAQQENAETDLYDNSASSQQLYALAEQLFSHQKSTSEKPEPCGQPEVWADGRQELCETLHYYRAYQGACYSTGGFVRGFMFDKVAHIRDYTDGNVVISRAGGGLIKDKDSGEMRSSRDQVEDIVAHNLRNCINHYNPVVIITGVDNPHIPSKPPQQYCVLDYFKPTHIWVEKSGGGKIVRYRFEKLNLKKDSWWRPKDETDAIELGSLPPPIKKACTSCGVVTSQLYLNGWMCLQPDCLMFWKIAGHGTDTEPQEPDEGSLVYDPRFLKQKTPWPNDDHEYSLVSNNAALSGYSVIGEDTSQAFWLGMVCPDCGRCIPRLSWMGWECQNPACGFTKEPPHTLVPAVTLREPLWPLTSQYTLSRDIQAPLISVCVYFANGYRINQYTIPGIDGFIAHMIANKAVIEEPGGPDAMFEELQQTDIGLRRRSMPNGQLKGPNYCRHFTVNYGMPYKFIAATASQSFEGAARPITATRSRLNWAAKLLLSQDSNRSMNDIEKQWKEKEFNEVLALGYFEQQKINYHDDGEFGLGPTIATLSLGAPGTMRIRMKARHYHGVSSAGVYDDAPPIPGCQQYAARLALQSSLDALKASDSKAYNARRKEIPKELKLRSSGQAKDVLNMQLGHGDIVVMHGADIQRYYEHAVEHAGKLRFALTCRYIDPGSLKEADKPDYEVGPDTEGYDGSGLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFY1</accession>
    <name>A0A9W8YFY1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFY1"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="88" mass="9850" checksum="45AA3982FE9F3613" modified="2023-11-08" version="1">MPSPSSIPQQQQQQFYGSLNQQTQQPLQQQRMQAPQRHPSFEYQPNRAGAGQTAAETTNYLNQAALLAEAAKRAQMACVMRDIGDMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGF8</accession>
    <name>A0A9W8YGF8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Anaphase-promoting complex subunit 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cut23</name>
        <name evidence="10" type="ORF">N0V83_000105</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGF8"/>
    <dbReference type="OrthoDB" id="10262026at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007192">
        <property type="entry name" value="APC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF10">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04049">
        <property type="entry name" value="ANAPC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cdc23" evidence="9">
        <location>
            <begin position="11"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="380"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="414"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="448"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="44"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4377281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="645" mass="72004" checksum="F1A0B5BA8E3FD72D" modified="2023-11-08" version="1">MGTLSPADEAQLKAQLQDAVVASTERCLFHSAKWAAELLNSLPSADDDGSDTDVDSPMSDSPLPHTPSLVPKDPTEARLEAREAHKFLLAKTYFDCREFDRCAAVFLPTALPKAPINASATPSKKSIQASKGKAKIGTPTKAKFPSESISGLSQKSLFLALYAKYIAGEKRVNEDSEMILGPQDGAVTMNKELPGVSAVLEEWFKNLPSSGRQPQGWLEYLYGVVLAKGKNEKLAMEYLLKSIHQYPYNWAAWQEIQGLLSTPEELNQLVTRLPQNLMTFIFHVTASQELYAVTDQIHSSLSQILSIFPTSAFLKSQRALLHYHNKDFDDAEQIFSDLLVSDPHRLDQLDNYSNILYVMGMRPKLAFLAQLATATDKFRPETCCVVGNYYSLKSEHEKAVMYFRRALTLDRTFLSAWTLMGHEFVEMKNTHAAIESYRRAVDVNRKDYRAWYGLGQTYEVLEMHSYALFYHQRAAALRPYDPKLWMAVGQCFGKVGKVMNGIRAYKRALVAGSYFEGGVGSSFGSGMASSLGGGILDPEVLYQIALLYERIHNMNECAAYMELVLAQEEGPDLDEVGSEGAGGVGVTATTSKARLWLARWEYMRGMYQRAMELANELCQDGVEVEDAKALVRDIRARMERDRGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGJ7</accession>
    <name>A0A9W8YGJ7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Galactose oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_000524</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGJ7"/>
    <dbReference type="OrthoDB" id="10250130at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019760">
        <property type="term" value="P:glucosinolate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47435">
        <property type="entry name" value="KELCH REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_5G12780)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47435:SF4">
        <property type="entry name" value="KELCH REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_5G12780)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="161"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4377695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="451" mass="48072" checksum="433F7F91473B10A0" modified="2023-11-08" version="1">MEPAAAGVLYAAESLLEGAAALVKGITHPTLPIRANLTRITSVPLPRSQHTVSIVKGRAYIFGGETENGRLADNAMHVVILPSSGVLEADYTTFPARSANGLDDVPNPRKGHTAVVIGDSIYVFGGEIDGLTNEMGRMWVYDTVSNTWSYLDPASGTLFPSKRTGHASASSNLPGPKTRTYHEKAPQAPADPATVVPEPAESDTWGTIFVVGGRDTMSGVFSNDALAFDVKSRTWSNIPSPPGQPRDGASLSLVGDRLYRFGGKGVETWTSGATKFLDVSHVWKHSEGGTTPLTSGWAWEEVSHTLSEGNEATAPQARSNAGLVGLTTGQGRHYLLAIGGETESGTFFDDIWALQLPPERSTAAATKDSIRASIKRDTHESQWAEVLYQYIDTKGEVEKEIPGEPKSGLGRRGHFGIAKGTEVDGATAVVWGGVDDQNMVLGDGWMVTVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGU7</accession>
    <name>A0A9W8YGU7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001751</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGU7"/>
    <dbReference type="OrthoDB" id="5367584at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="288" mass="31532" checksum="65A8C3259D9C431B" modified="2023-11-08" version="1">MLSYVSNMAEPMSTLSPEKLNANSNSMLIDDDFDITFHDPPSSPFVSHIENDDQENIAPNVAPTPSKPLLDFSDDVPQSAFKVSPEKKFGVTQRASPMKNLMGDFEEAALKSNVDSQRSPRKSSPIKQIAAERPESAMSSHSHKRQSLSSTPRAPSDESIQKPSPFDDSNPEIAPMPSKRPSSSHNESALRDNEGLTVAMRYMEETRTESREILTRQISQESDFGVDLGADNTDFNPDGPDLTSADMDDTCFSNFSEMPGLDMTKFAFLKQSPAKGGLQEVGICGCTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGX7</accession>
    <name>A0A9W8YGX7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar protein sorting-associated protein 74</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">VPS74</name>
        <name evidence="9" type="ORF">N0V83_002142</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Mediates the cis and medial Golgi localization of mannosyltransferases through direct binding of their cytosolic domains. Involved in vacuolar protein sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">Golgi stack membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GOLPH3/VPS74 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGX7"/>
    <dbReference type="OrthoDB" id="2189106at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048194">
        <property type="term" value="P:Golgi vesicle budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3630.10:FF:000002">
        <property type="entry name" value="Vacuolar sorting-associated 74 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3630.10">
        <property type="entry name" value="yeast vps74-n-term truncation variant domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008628">
        <property type="entry name" value="GPP34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038261">
        <property type="entry name" value="GPP34-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12704:SF2">
        <property type="entry name" value="GOLGI PHOSPHOPROTEIN 3 HOMOLOG SAURON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12704">
        <property type="entry name" value="TRANS-GOLGI PROTEIN GMX33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05719">
        <property type="entry name" value="GPP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4375062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="350" mass="39041" checksum="B4F2808C0F13272B" modified="2023-11-08" version="1">MASSSGLTRRRGGGGAAGNGEDEGSRVSSPAPRRNDDRTPETSYEASENGHKIAFDPRDISESAERSKQPKLTLMEEIILMGLKDKQGYLSFWNDNISYALRGCIVIELAFRGRVSMQKDSSRRRFPLADRVIEVIDDTLTGEVLLDEALKMMKSSEKMSVNSWIDLMSGETWNLMKIGYQLKQVRERLCKGLVDKGILRTEKKNFLLFDMATHPVADGGAKEEIRRRVRNVLTNRTVVLPGSQFLPEELEFRYIRTISMVCAAYAANVLENALTTLGHEARERAFAQVDELLAEYSQWPFARRQGGSQGVGANLGQVIADEVNGSKDKELQLEVVAACLSVFTRLDSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH06</accession>
    <name>A0A9W8YH06_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_000304</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH06"/>
    <dbReference type="OrthoDB" id="296386at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049456">
        <property type="entry name" value="Anoctamin_N_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF73">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20877">
        <property type="entry name" value="Anoctamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="332"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin alpha-beta plait" evidence="8">
        <location>
            <begin position="24"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="7">
        <location>
            <begin position="178"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="705"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="711"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4377479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="724" mass="82860" checksum="733D07C50FC71D16" modified="2023-11-08" version="1">MKPLRRAETTFSDDTALANITYNDKYVVVYDFSKVDDDVAIKEFTALLKDLQSVGLNTEVRPGYDQSLLVFIQAPRELLGNTVYKSRVKDWLYGITKHHPGGNASSSVSAAFEAEDVLSMHHLVNWRKDLGGAGVTPGFDRWENVTAIFPLHNHRTNHQLLAHLSTRIFLSLEDLDQIRDLWGAKVAFYFAFIQTYFRSLAFPCVAGILAWVLLPKYSLLFAVVIGIWCTVFLEYWKIKELDLALRWNVRGVGALKTNRPQFRYEKEIIDSAGRLRQHFPRWKRILRQSMVIPFVLVSTLVLGILITFVFIIETFISEAYEGSYKFYLEYFPTVLLAIFLPYATNILETTAATMTEYENHRTDDSHEMSLTQKIFILNSVTNYLPIFLTAFLYVPFGGQLIDLLEDFIDRTLGGKRWSDISFKTDPERLRNEVIALTLTGQVSSAVEELVFPWLKMQAKQWWRDRQAAQAHRRGVSGYQTSADDPSEAHFLRRTRKQALRPIYNVQDDIAEMVIQFGYLALFSPVWPLIPIGFFINNWFELRSDFLKICIEYQRPAPSRSDGIGPWVASLESLTWLGSISSAAIVHLFGSHKYIGHYMGLSTWASLPITILISEHIFMGFRAAVRFALAKIGSEQTRKESAESYARRRKYLDELEANADKPSRLDVTERERRKSVRINAADLFWTRQAEEGTSERTGISIIKLAKGAQAEAKADGQDNKKSKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHF9</accession>
    <name>A0A9W8YHF9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Autophagy protein 16, interacts with Atg12p-Atg5p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ATG16</name>
        <name evidence="5" type="ORF">N0V83_001587</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATG16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHF9"/>
    <dbReference type="OrthoDB" id="8949486at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013923">
        <property type="entry name" value="Autophagy-rel_prot_16_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08614">
        <property type="entry name" value="ATG16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Autophagy-related protein 16" evidence="4">
        <location>
            <begin position="7"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="34"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="83"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="198"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4376304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="217" mass="24442" checksum="FC291055E0831366" modified="2023-11-08" version="1">MSNPLAEYLSALESRDAREKAHEEYINAYTRLADRTASRVNQASPAVDEPAPSPSSSAKTIRAASPKAKGATAPAQVSSPSALAQIRAELASTQRTRSELETKLSTLTTELSALKTTDTQRRQRIEQLERAKTALERRGKDRADELKGKGRFVEEVQDEMVALNLQLNMAEQEKEKLKKENEELTRRWVQKMEEEARKMNDRMGWEEQGGRRKGSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHI2</accession>
    <name>A0A9W8YHI2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_000281</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHI2"/>
    <dbReference type="OrthoDB" id="5361565at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040993651" description="Peptidase A1 domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="411"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="4">
        <location>
            <begin position="45"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="450"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4377456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="486" mass="53887" checksum="4685EB7FA0C3EEF8" modified="2023-11-08" version="1">MASLLLCLSATVLFVQSTVAFNCSQKPIYVDIHKRAVHDSPVFQYGSFIGLGTPAQNQSLWPSLQQNHTSFASPTYCKDNSTLKNCDRSTGGFYNHQDSTTFIENDNFKTLDQVETDFKSFFGQETLRLYTHFFETDGASQTLVENSTIEVAEQGSITPARVGVGSSSTLLRDLAAQNIIAGKTYSLYIGQGFDRAGGVVNGSNVFGGYDSGRFTGTPNQYPMNIANVNPMSVRVKDIIITDSNNANVSLFDNTVFTDMKSRPDEFEAQITTEQFPFSFPYQITQNIIAKLGAVQDNTWGDNSLKLKNAFNGTLSIVLDDGFTVTLPPEVLMNASNITPIQDRDEKSTAPFYLGTAFLGQVYLMADYESDNFFLAPAVQKNNMVMPVTFCPKSTPVAYERPNQSAWQRQGLIGAVVGGVLGGLGAIAALYCLWITWMRKKDERKLKRELKKNRESKMEQMDLEDAPTFDPPPKNAAKAIFWRRNKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJ82</accession>
    <name>A0A9W8YJ82_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000106</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJ82"/>
    <dbReference type="OrthoDB" id="3690858at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052594">
        <property type="entry name" value="J_domain-containing_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44144">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44144:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="214"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="333" mass="38493" checksum="ED878D8F014EC420" modified="2023-11-08" version="1">MIITVNRIYDIIVARHGSLDLLHLRPADLVFLQRARQRLLVDQPQQDIVLAPRPQQIVVQQQQPDIVVPPPPEPQKIIVQQQQAPPPIVVQQPQPIIVRQQEPQRIIVQQRQGPQIVYQQQANLIQNPPGPTLSNAEEEEFKRRFEEEERKKREREDTLRRQFEEEEDFRRRHEQEQRLTARNPESSTPIPRTIEPDSDSDSDSDSDEDEKKFDPYEALGLHDRQRTSLDVIRSTYNNLVLIFHPDKQIGKTAQQIKHAAKRMYDINKAKDILLDEERRRAYDEAGAVFEYEFEEWKRMTSKCGGLCGEKIAVANGDLGGAGGGKGKNKYIPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJB1</accession>
    <name>A0A9W8YJB1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_000375</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJB1"/>
    <dbReference type="OrthoDB" id="47494at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47178:SF2">
        <property type="entry name" value="FAD-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47178">
        <property type="entry name" value="MONOOXYGENASE, FAD-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="6">
        <location>
            <begin position="123"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4377550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="407" mass="45113" checksum="84843A889035362C" modified="2023-11-08" version="1">MAADDRKNLHVLIVGAGTSGLLIAQGLKQASIPFTIFESETTSTYQTRPREWGMTLHWGSNHIASCLPPELVERFNEAYADPLQKPDAATGLPIHNGKTGELIMEMAAEKPYRVSRKKMRNLFKEGIDVQYGKQLENASVGEDGKVKVEFKDGSSATGDVLVGCDGAKSRVRECVVGVAAAKLTNVPVSMFNFPYKFDAELSSRIREENQLFTTSIHPDHGTMFWLSIQDVPDPNKPETWTFQVLQSWVDDTVPASADLTTFPGRLAFFKSRSAEWAEPWRSAGLAVAEGTQLPLDQGTYWERAAKWDNRSGRLTLCGDAAHPMTPHRGQGLNNALQDSANFVAALRKVKSGEVGLAQAVQEYDDEVLERGTLEMNISLKQTLFIHNWETLMQSPMVKMGMHQAKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YKQ5</accession>
    <name>A0A9W8YKQ5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Antiviral helicase ski2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SKI2</name>
        <name evidence="12" type="ORF">N0V83_000908</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="64767at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055087">
        <property type="term" value="C:Ski complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070478">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000354">
        <property type="entry name" value="ATP-dependent RNA helicase SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1500.20:FF:000001">
        <property type="entry name" value="DEAD/DEAH box RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000987">
        <property type="entry name" value="DEAD/DEAH box RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.30:FF:000001">
        <property type="entry name" value="Ski2 ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1500.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025696">
        <property type="entry name" value="Beta-barrel_MTR4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048392">
        <property type="entry name" value="MTR4-like_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016438">
        <property type="entry name" value="SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012961">
        <property type="entry name" value="Ski2/MTR4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040801">
        <property type="entry name" value="Ski2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE SUPV3L1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08148">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21408">
        <property type="entry name" value="MTR4-like_stalk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13234">
        <property type="entry name" value="MTR4_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17911">
        <property type="entry name" value="Ski2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005198">
        <property type="entry name" value="Antiviral_helicase_SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01142">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="325"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="609"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="208"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="525"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="208"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="543"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="580"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4378077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1290" mass="144605" checksum="FF888475043EDECA" modified="2023-11-08" version="1">METLSGELEKLQLSSNALDRDAGWIDSIAGEQRPPKRQRKTKEAIKKELEHEFLTPSRSFNTQWLNQLQQRWDAPTDYRSLFQIAPTQTRTIIRFTREGLEGRVTGYKEVTVPANSATAKNSTSFLRKPANRAEFVRGAAGFFPFAPGGLDGVEAIAAIEDEAILQQEQLNAKKSGGLDRVINFSAEGGLLEIPPGFTRGLDFKKKQTTDAKTAKEVEDTLGEASAVAKRTEADEEEEDSAGADENGVHDEEEVPGEEEIDALLPVEFPALAPHGTLAIGAGRKGGREWAHMVDVNRDITNFRELVPEMAREYPFELDTFQKEAVYHLENGDSVFVAAHTSAGKTVVAEYAIALAAKHMTKAIYTSPIKALSNQKFRDFRLTFDDVGILTGDVQIRPEASCLIMTTEILRSMLYRGADLIRDVEFVIFDEVHYVNDLERGVVWEEVIIMLPEHVTLILLSATVPNTYEFASWVGRTKKKDIYVISTAKRPIPLEHYLWAGKGMHKIVTADKKFIDNGWKDANDALSGKDKVKAPPAAKEAPTGRGGGGRGGQAGRGGQNQRGRGQQNGGRGGGARGGGPPASRGRGNIARTGRGGGRTTAAQDRNIWVHLIQHLRNKELLPACIFVFSKKRCEENAEALSNLDYCTAAEKSAIHMTIEKSLARLSREDRDLPQIKRLRELLSRGIAVHHGGMLPIVKEVVEILFAKTLVKVLFATETFAMGLNLPTRTVVFSGFRKHDGREFRDLLPGEYTQMAGRAGRRGLDSVGTVIICAPGADEAPPAARLRQMMLGEPTKLRSQFRLTYNMMLNLLRVEALKIEEMIKRSFSENATQALLPEHQKQVKISEADLEKVKREPCKICDIDLEACHQACEDYKRLTNEMHLSLLINPVGRRVLGKNRLIVYKKDNKRTAGMLLQDGTSKGNEPTVKVLEVAQHQERKADDLLPYLSPFAKYFRTLPKNPKDLILKTAFVPLNDIECFTKAIIDIPDSVQNLNRKKDTLKLAQDQFLPLCQSWNYEDWDEYDYGRINSLKFRELEDARKREGQNAVNKECLKCPDFLKHFSMEHDQWLIKENILSLRQLMSDQNLQLLPDYEQRIRVLNDLGFIDEGSRVELKGKVACEIHSADELVLTELVLENVLAEYEPEEIVALLSAFVFQEKTDIEPTLTASLERGVAKIVEISEKVNQIQTLHQVILSADDSNDFVSKPRFGMVEVVYEWARGMSFNRITDLTDVMEGTIVRVITRLDETCREVKNAARIIGDPTLFQKMGTCQELIKRWKCAGNMADPSGQWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHG8</accession>
    <name>A0A9W9CHG8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Trafficking protein particle complex subunit 31</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">trs31_2</name>
        <name evidence="2" type="ORF">N0V83_010419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHG8"/>
    <dbReference type="OrthoDB" id="3796753at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="170"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="401" mass="46246" checksum="30BE13B7D3B424BF" modified="2023-11-08" version="1">MLRDYRNLFLAESDAEFLRMLCVDMYTDARKQNPPAQKTLFPHAHPRVAKPQALGNALIPTSMSFDLDALFLERLELFAEPQPTESRRVNSGGKETPQICEKINHAVEEAMEKGVVDNNLRDSIQTLIHERDNWKEQAESQSEVIQKLHHRIYSKNIANSDLPADLVRRMDRVESENVRLRADNMQLKENLRAAESENATLCDENDGKNKKLKGANKKVKNAKNVAGKEEVKAKDAVLDKQRHLTSERKMKKERNDALAALEEQRKINESLRTELEVEKSGVPHMRDNETKPDITTVIVPVQFEVHRSHFRELLMGLEAYQMAFVDKMKCWYEEWKKPEEETPKVVGANYLDDGKKARIYEDMVEMPEGYLQTGAEHDGWRSKRGLEAVCRYAETRHSHQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHV1</accession>
    <name>A0A9W9CHV1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010309</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHV1"/>
    <dbReference type="OrthoDB" id="5587021at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040845645" evidence="2">
        <location>
            <begin position="24"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="269"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="292" mass="29811" checksum="ADB7D2C548D54365" modified="2023-11-08" version="1">MSFSTIRLLLISIFFTFATHVFAQTASSAASQPSTPPITSSTLPRGNGTSPTAPPVVGPTSSSPPDVLLRVPELHVGRIELDVERLQADLNLNAKVAGLVTVNAGVQVSVQKVNITIADVDVDLELVVRLGNLVSIVERVFDSLDLNPLLISLIGNVTNLVGEVIGAVDGLLGSITQNGKTLNFLVDNLGNIVQEVVGGAGDAISQIVGNFKLNMTEVAGTVKQVGQGLTQTTYSYGALSALVDIVTNTAGQVVSAVVQKKTGSGGGTTASPTASGTPMPVSLPVSSATSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI64</accession>
    <name>A0A9W9CI64_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_009983</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI64"/>
    <dbReference type="OrthoDB" id="10257314at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051323">
        <property type="entry name" value="AtsK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468:SF10">
        <property type="entry name" value="TAUD_TFDA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="7">
        <location>
            <begin position="32"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4363686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="175" mass="19732" checksum="57CB33AFAF0870B3" modified="2023-11-08" version="1">MKSNCKRRLACRVNHFLNTSFVKLYLREQSITFERIPSNYAILKIHTLPTDGSGGDTLWASGYEAYDRLSPAFKTFAETLTATHHQPNFNHVAEEFGAELIDNERGAPDNTGLDFKASHPVVRTNPGTGWKSLFGAAHQVGHGWIDGVSERESDILKAYFLQIIAENHDLQVRFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI79</accession>
    <name>A0A9W9CI79_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sphingolipid delta(4)-desaturase</fullName>
            <ecNumber evidence="8">1.14.19.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">DES1</name>
        <name evidence="12" type="ORF">N0V83_008719</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Delta(4)-fatty-acid desaturase which introduces a double bond at the 4-position in the long-chain base (LCB) of ceramides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>an N-acylsphinganine + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = an N-acylsphing-4-enine + 2 Fe(III)-[cytochrome b5] + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:46544"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:31488"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="EC" id="1.14.19.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the fatty acid desaturase type 1 family. DEGS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.17" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI79"/>
    <dbReference type="OrthoDB" id="200948at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042284">
        <property type="term" value="F:sphingolipid delta-4 desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03508">
        <property type="entry name" value="Delta4-sphingolipid-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011388">
        <property type="entry name" value="DES1/DES2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013866">
        <property type="entry name" value="Sphingolipid_d4-desaturase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12879">
        <property type="entry name" value="SPHINGOLIPID DELTA 4 DESATURASE/C-4 HYDROXYLASE PROTEIN DES2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12879:SF8">
        <property type="entry name" value="SPHINGOLIPID DELTA(4)-DESATURASE DES1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08557">
        <property type="entry name" value="Lipid_DES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017228">
        <property type="entry name" value="Sphnglp_dlt4_des"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01269">
        <property type="entry name" value="Lipid_DES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Sphingolipid delta4-desaturase N-terminal" evidence="11">
        <location>
            <begin position="36"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="365"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="366"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4365101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="395" mass="44483" checksum="0ECB31BB64D7F6D4" modified="2023-11-08" version="1">MRSSVSTATAPPRKPAGRFTVQAGKQQRASSSEHKHEHDDFFWTYTEEPHRTRRMAIIKAHPEVTKLCGPEPLTKYVVLLVVSIQILCAYLLRNTPVLSWPFFLTAYIVGATANQNLFLAIHEISHNLAFRNALANRVFAVFANLPIGIPYSASFRPYHLTHHKSLGVNGLDTDLPTAFEAVFLDSVAGKAFFATFQILFYALRPMFVYNLPLTQIHLFNVVVQLAFDYALVHYAGGKALGYLIMSSFLAGSLHPCAGHFIAEHYVFEKPNNREAATDPANKIPLPETYSYYGPLNFFTYNVGLHNEHHDFPAIPWTRLPKLNQIAHEFYDGLPQHKSWVYVIWQFIWDKDISLWCRVKREEGGRKVGAGGSGGGAWSEEELGANERKGPIPGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI92</accession>
    <name>A0A9W9CI92_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_009866</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI92"/>
    <dbReference type="OrthoDB" id="2123952at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052202">
        <property type="entry name" value="Yeast_MetPath_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782:SF1">
        <property type="entry name" value="PYRIMIDINE PATHWAY REGULATORY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="13"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4363570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="675" mass="76605" checksum="5FBED0EC378B18E4" modified="2023-11-08" version="1">MQNRTKVRRRVLACARCRKRKLSCDGKVPACTRCVESGVQCVGFDSSTQQEAPRSIADFLEAHIAKLDVLRSPSSLQALGRPSAAIPSPADSHSSEAASGQWRPEKCAFADSLVNQVMEDITPSFLGVSKVRPLLQCVVKGTQLPSKKGPVVSTDLNENHPRSILNPQPTKGLFHDLMPDEVGANTLLQIYLDRVITQYPIYHRNDVTIAFNSIYHKKASTPGEDSFRNRYIVCIIMAISLSTSARHKVQKANETAYQLVRHAMQWIPEVATNDIPGLQAILLLTQYIFLNPRMADLWLLTGLISQAVIDLGLHQELPNSSRVSAYQRDMRRRLFWCAWEMEVGVCCIFLRPTSLPIKNIEVAFPLEIDDTAITQASMEPSGRVSKFTQRIICRFRLIEAEIISVLWLGEPVPKEYASMEQWIDRCINAMSQWQQEIYASRDANKDRGLNARWNEMCLYTDIACPYILATLYRSSRRIPNPTTSQTMIALVNAVKVADGYFRQSEAEAGKIKYVFHPCHHVFNCALIFLQGLQRCKQEVSENFTWQRVEEWMHVFAKCFSSIAERWAAAKRCLEEYERLLLPIKKEYMEFLTQKASFLHPPRHPNTQMTGALFEYPGPAPAPAEIDEAYQFWSVFNPTTTTADTEALGAYVYNVPPRDWNAEFSLNYGMESMPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIE1</accession>
    <name>A0A9W9CIE1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009402</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIE1"/>
    <dbReference type="OrthoDB" id="5276978at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4363949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="179" mass="19806" checksum="730F4320956BBC61" modified="2023-11-08" version="1">MAKAAAETPYREVDPLMPWFAHLCHAWPRAKQNPGDWADESSTYAWDLSLAASKRDTHACHPSQRIRTYYLTPTDPKLPHQTAPAPMATASAGSNIKLLFGGNGHSRGDNVGGHGDPGQVIVYWKGEAGAEIVDVSEFRDEFKVQENGFAEESFAWPPDKSVTKPPDLRDKGNWMTLKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIL8</accession>
    <name>A0A9W9CIL8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009614</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIL8"/>
    <dbReference type="OrthoDB" id="3687237at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040985104" evidence="2">
        <location>
            <begin position="20"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="507"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="753" mass="80059" checksum="6AF84BDDC6A9455F" modified="2023-11-08" version="1">MARFLSLVVWGTLTALSLAEVFTLSLGDIGKYSTSLTATPTLTGSRVLFTEPANPNDGVDIVLSDELRANIDKAIADSCKNVDSQCVESIKNLLVNPHTELEARQLVAAGAGLFALLALAIPLYEKERSQGVPVAIHIPSAQIDPAASAAQASTIAAITGSGSPLITITQKPDLASVTATKRFRERALGDQYVAMACASEQIALNSSPGGAFAELIELQGPQPAWTSPELMQALGGATTWAVAKAPTMKYSSAEAKQIISVGFMLSFIVIILKQPLSGVNWISGSELRGSMTLSPMPTTISVIPTSLVTTSTVMSTSSVFECVASCKMIGHIKSCNTKCPTATGAPIPKPTNYAVKTATIERWVVPYLPQTPLQAPVGLCIAAEDTNYPIDLFAKTYSDFCDQADQSTSDISWTVNAQGQQIARKLRRFVPRGGAADVDEYKDYSFTLWWRPVAGGSGCAESCNDAFEQLVTSDSCKQGSNKSNMASRGSIDLGCGAYSFAINSKPAAESPKSSTKCRNHPLSAPKHDANANGATSVDAAAQRWCSDNDGHALTGETGKNEVYFRWGITQLSVPNRSSFWLRASLNGDNKQGKVIKSECIAALTDSLAQCDVDKDVTHGFTASVGSIDYSLDLSGVTVDGNPPWNEKPAFPAPEFTPGKDLGGAAQSPTCYPSDAELGRKVSGVDLESAMNAFCVNGADIAGFGYHWDKMFQYPPKGQPQFYNSASTKMHLEFGAETINNGAKEPYNDMSWCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIX4</accession>
    <name>A0A9W9CIX4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_008613</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4364997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIX4"/>
    <dbReference type="OrthoDB" id="10016252at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050641">
        <property type="entry name" value="RIFMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43004:SF19">
        <property type="entry name" value="BINDING MONOOXYGENASE, PUTATIVE (JCVI)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43004">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="7">
        <location>
            <begin position="28"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4364997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="436" mass="47331" checksum="21E60A284014FC4E" modified="2023-11-08" version="1">MAITIPSTSTNGHTNGHTNGHTDSPHHPIVIAGGGCVGLFLALLLSHSAIPNRILVIEPFPPDPTSTRAIAHQPPTYPILSRVPGLLEELLQTGSLSSGLCFRTSVANGSKVIAEKRFSNGEVSGVLMKRLDVLGRDKVEVRVGWKVVGFEQSETSVRVDIVPTSSSSPSLAGEGQNHPQQEQEHLEAAYLVGADGAHSTIRKIFDVDFSGETLDAQLVATDIHFDFKSHSFHDANFIIDSLHYGHISRIADSGLWRVSYSIPLGLSEEEIKQGVDAKLKGMLPNGGMNEMGEKMYEVVRVAPYKAQQRCVETFVKGRVCLVGDAAHLTNPYAGLGLASGMADASSLATVLAQILTHQASDEEALLKAWSEDRRKKFFEVVDKPSRMAYKRVRQDVSTDEKIQGLLSRDPMVGALKKGMPVMPPSLQTVEKELKGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL14</accession>
    <name>A0A9W9CL14_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Xylose isomerase-like TIM barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006471</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL14"/>
    <dbReference type="OrthoDB" id="5360893at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050312">
        <property type="entry name" value="IolE/XylAMocC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12110:SF38">
        <property type="entry name" value="DIOXYGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G00240)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12110">
        <property type="entry name" value="HYDROXYPYRUVATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="1">
        <location>
            <begin position="29"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="334" mass="37292" checksum="3BFB4EA99BF782C2" modified="2023-11-08" version="1">MRTFDIKPAIGTSSLGLHPSQSLDDKVLAAARHGFTGIEVVYDDLERYSASQKIDMALAAGRIRRLCLENGVDVLALCPFENFEGHKSPLGERLAAADRWMKLARILGATYLQVPAQYGKDVVGDETVIVSELQQLADLGSSTEPVISIAYEPMSWSIFCSTWQDALRIANLVNRPNFGLCLDSFHIATKLWGSPYNTQGKYPDGDQLLARSLREFVESFPLEKLFYIQLSDGERFDPPFSEKHPWYIEGEAAEFTWSKHARPFPLETECGGYMPVTEIVQALVVAKGFSGWVSLECFDRRTRDASVQPEDCAMKAGRSMKRLQSALQAPKSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL86</accession>
    <name>A0A9W9CL86_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006697</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL86"/>
    <dbReference type="OrthoDB" id="3034343at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001080">
        <property type="term" value="P:nitrogen catabolite activation of transcription from RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050797">
        <property type="entry name" value="Carb_Metab_Trans_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668:SF10">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="2">
        <location>
            <begin position="212"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="566" mass="63858" checksum="6949D7C457B714B3" modified="2023-11-08" version="1">MAADRRRGNVKAMYQTMQAWQFLPPLNMSSLMMNPPMAEDIAILQQYLTSHTSEGNPAAKLYNTISNAPGNPIIYLTVPRRRKGLNTAADPGRTQREIIEQILNPFAAEVRRLYFDRLHPCFPILDEKTFSDMWKRDNDRISSTLLCDLYASALSFWDCSELLQAYPRPDVQFVWNQAVAALQDDFMAPTISTVHAALLDMVGRPVIQVTGNIVNAGRIATLAQSLGLHRDPTLWKATAHEKSVRIKLWWGVLIHDYWSSLGHGIPPTTNPRYCDVPLPTPDMLATSIASETQKKATTTFIHLCKLSQILGDVLPFVYSLQLDEEEVSRSLRRTECFLDDWAIALPEYLRSDSSSYSGVNGASNLWFSYLSVKVLICRLAFKTTLKNSKASFSEARQYRLALLREASCDVLDFVTSLKEAQLREFWMPYTSYLLVTATTILLRCTIECGAVETKRVCAMKLVHFLDRLRDASKNSSWDLADFCLERCHEPIQKIADALKIPSQTSQAQIVEANLMNASSDIADQVGLETATSGSAPMTDFFLPIDSLDYPWETLWDTFEGPWPIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL91</accession>
    <name>A0A9W9CL91_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006578</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL91"/>
    <dbReference type="OrthoDB" id="4146887at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39609:SF1">
        <property type="entry name" value="RFEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39609">
        <property type="entry name" value="RFEG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="158" mass="17784" checksum="3F99ED1E466B6403" modified="2023-11-08" version="1">MSRSNQWWIPGEGIAREVITEDIQRYLGPDALVRPGTGTGEYEGQNGYWITAYRTLTSQMIQDLKMDSQRWQMENTGSRSSYQDSRTHAARQHWGPSSVTTVSGPAQNPYNQSSSISQLAGPPPADPYRNPYTVPAYEPYPPYATYSQSGYPVTIRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLH1</accession>
    <name>A0A9W9CLH1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_006819</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLH1"/>
    <dbReference type="OrthoDB" id="5416384at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14723">
        <property type="entry name" value="ZIP_Ppr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052202">
        <property type="entry name" value="Yeast_MetPath_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782:SF8">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="34"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="613"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="663"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="614"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="675"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4368462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="814" mass="91927" checksum="CD5798AEE8B3B462" modified="2023-11-08" version="1">MEAATATQAKTEGAPHPSEEGTAGQSRTAHTLTACCRCRTRKTRCDPGLPRCGPCERTNSHCEYYDPAKGRKIPRNYVVHLQHKVRELEKQLAELDKDDVEPDPEDVMRGAATVRVQDSDESKFLGPSSGITVTRLVMQLAKQFTESKSISEIVPHARAKSIKATFAQEDERPTSKIYPLVSDVAAEELPNRDLTNLLVELFYCKVHPMYPIFHEPTFTKDVEDVYRGSTDPYQNYCLRMVIAISLQKMDTQYAGLADSYYLAALKFFEAAIKPMNLKTLQCFALVAGYSLLTPTRTAVYYIMGLGVRLCQALGLHEEKTITRGPGGRPADPLEVDMRRRLFWSILTMDYGLSHSLGRPAHFATRREHIDVNFGELVDDAFITKEGIKPAPQASLKKWIGIHFYKMRLLQLEIRKMLYQRKKPEPKDDEHPWFSEIQAKIEAWRDTSPEMDGGSGLNKVWFIGRYNTMVVFIYRPSPQVPRPSVQAAIRCYDACQYNIYMTRKQIETKSVDMTWIFVQAIFMCINTMLWTLSYSEVRRVHSREEVEGHLNVAMESIRLSSERWPGVASAMELYYNLIGACMKIFDKDGDVPISASSPSDTASVVSSNIVDGINRSRTTSPATASTASVSTPSDKVPAPFGYLPNQNQPIFNFSNSTNVTNPFSTSPNAQHAAIHTSPQQSTSQPYTDMSPKASMDTNISIYTYPPTTQFTSLPTTFAELPQWTPTFSMPQQDPYGMPTMPMSSSFYNEAYAANQGYEVADYLNPGWSQDARGNGLNQEQQMQLMHDFEMNEAKNIEEMIQQSHQLFRPPQLQTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLJ5</accession>
    <name>A0A9W9CLJ5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Mannan endo-1,6-alpha-mannosidase</fullName>
            <ecNumber evidence="4 10">3.2.1.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_005992</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Random hydrolysis of (1-&gt;6)-alpha-D-mannosidic linkages in unbranched (1-&gt;6)-mannans.</text>
            <dbReference type="EC" id="3.2.1.101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyl hydrolase 76 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.101" evidence="4 10"/>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLJ5"/>
    <dbReference type="OrthoDB" id="4187847at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008496">
        <property type="term" value="F:mannan endo-1,6-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000006">
        <property type="entry name" value="Mannan endo-1,6-alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005198">
        <property type="entry name" value="Glyco_hydro_76"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014480">
        <property type="entry name" value="Mannan-1_6-alpha_mannosidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145:SF36">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03663">
        <property type="entry name" value="Glyco_hydro_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016302">
        <property type="entry name" value="Man_a_manosd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040917826" description="Mannan endo-1,6-alpha-mannosidase" evidence="12">
        <location>
            <begin position="23"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="427"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR016302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4368910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="449" mass="49048" checksum="DC5810A633834A58" modified="2023-11-08" version="1">MKFSSTLGALGASSILLSGTAAITLDISSPDSIKEAAKSLSTNLRTYYTGDKLGDTPGNLPDPYYWWECGAMFNAFIDYWYYTGDDQYNTMTTQAMEHQIGDYNAFMPENQTKTLGNDDQAFWGMAAMSAAENKLPDLGDGKPSWLALAQAVFNTQKSRWDQTTCAGGLRWQIFTFNNGYNYKNTISNGCFFNIASRLYKYLGNDTYSDWAEKAWEWELASGLMSSDYHYYDGTDDTTNCTIINKIQWTYNAGVHMAGAAAMWNVTQNDTWKGRLTGVINATSVFFKDNVMTEVACENNGKCDVDQRSFKAYLARWMGYTAIVAPWTRDSLDPLLQASAQAAAKQCTGGTNQTSCGLRWIDNGVNDGSFGVGEQMAAMEVVQALLYPQVSGPATQKVGGLSESNPDAGADAPTTAVIFNSITTGDKAGASILTLLVLVGILVGAWWMVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CM57</accession>
    <name>A0A9W9CM57_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CM57"/>
    <dbReference type="OrthoDB" id="3790026at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4370526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="154" mass="18062" checksum="268D8FAF47DE9383" modified="2023-11-08" version="1">MSSPTMPVTDQLREILQVNALCEIQAWFADRGINPEDVLAKYNSDGTLKEPPAVPTDERSRWQKLNLLKFEYDGVRVHRICHFVMETQLEHWRNNPYRLPVFYYVATFLMLMVQPFGLIVMYLVEAKDHIGAGEENVLMPMMRGLWGLFRLWLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMA2</accession>
    <name>A0A9W9CMA2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005704</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMA2"/>
    <dbReference type="OrthoDB" id="5385910at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="232" mass="22994" checksum="FF5193BA81E4931A" modified="2023-11-08" version="1">MPPIPIHKDAPIAPTKADGITPQTAPPDPLPPTRTTPAYAPATTTQDSSNNPPAPQPGARPAAPTQNNFDASSPAPPQPGAVPTATVITTETRQLSSGPPPQFTSPPPSDSALRAGRSTTTSTTASKPGPTTLNYGLAGAAPSPFQAADSSAAPAAEPTRSSLEHPPGYVQAPDNAPYTAGGGIGGISGGDGGGGQGSEDAGVGAAAWNMLSKAGEALKKGEEAAWRAMRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMD7</accession>
    <name>A0A9W9CMD7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_005794</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4370030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMD7"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="482"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="506" mass="56079" checksum="18037F7A642FD790" modified="2023-11-08" version="1">MSDTGFVRAGPVNATNDDHRQALARLDQVFQSNRSANAEIIVDMTHPVTTAPDQTTGSHEKNEDDDKDKDKNQNNNGEKEKRFKDENENIKEDKHDRTHNARLWFPAREQTKADLSEEPRPCDSEIHKRLLIPFFLAMIMLILSVCYILGPGAKFIDDRAVRLRRALVSYALLFGHFNAHKALAISKAVRLGFPPFFGCGILGYCLALRRSGTGGDEEVQGVISRTRSDFDYWNASKAPLTIEIMDAEMSAEKPPRTAGILSNPGEAAILVAQFGIAIHACMVTGDLSTLLLFMTCKFLMDTIACLSLCTTGRFTACNGCSGGNAAYTWICGLIQQEIIIIRGAHKDLWNLGNPGSATVPRKNHYGTIENRVAIMVSLSLITITYLAKLLSVSASASLLCIMVVGAFGHLMIIDLLRSFGKSDVALKSVEIIQDDNGSILALQRLEEKYPGLGEQLVKKMFHDGLRGEEKASWEKFRVEREEQKQGRTLETEISEKDKVVSKNTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMF7</accession>
    <name>A0A9W9CMF7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004911</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMF7"/>
    <dbReference type="OrthoDB" id="4838853at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42024">
        <property type="entry name" value="AMINO ACID PERMEASE_ SLC12A DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42024:SF1">
        <property type="entry name" value="AMINO ACID PERMEASE_ SLC12A DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4370393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="380" mass="43069" checksum="332F8FF479B1E195" modified="2023-11-08" version="1">MATQHSSTEPLRPVEPPPPAATDYAPRPLTAQSNTLQVPGIQVDGASDDDERGRSTEARAATPVNNASLNPSVNPSANVSRATSPNPHHIYPHTQITYDDPDFRRPPPLKYSLRTRRKSIFWFWTLIFVDCILMPIGMYFGMWYGLTRDQLSANAVFSISTALLGTVSIVEYFVRFHRLWKKNSHCRVIGAKRYYLDWFHWNLSVGWFFVMIELIAGTCPHDPPIRVLAMPAPTMLFAIGVELLLVDILRIMKVPAPCRISSLPQGAPLRPGIYAYIEDICAVDGSGGTEFRQRLNLRYQASKAFREMLHRQTLFWAIGAIVCSCATTIIVFTIQRDAAYVLGWILPFLWAGVWTAITIPWVQRELTKEYEQWTTAKWKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNN4</accession>
    <name>A0A9W9CNN4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNN4"/>
    <dbReference type="OrthoDB" id="5428863at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38663">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38663:SF1">
        <property type="entry name" value="L-ORNITHINE N(5)-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="727"/>
            <end position="861"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1242" mass="140755" checksum="50D10B5710B69535" modified="2023-11-08" version="1">MPTPPSTPGNSDCGCNTHSVPDHAIDMLILDADGANWMAKWNRLFKTFGIDYLRSPMFFHIDPSDRDGLLGYTYEKDRGKDIQALPGCAGKEISKHRKKKKLNTKGRFLGRTPDVDERDRKDYFVPKTDLFKSHCEEVIQRYGLGKDLLRQERVTDIEASFFGDAEHDSVISDDLLDDDGKIFRITTDQGVRFAHVVILAVGPGNAPSVPVVPSLPSQNPHEGYTHAMQLKQFPPPHVSAKIKLRRSTNMLIVGGGLTSVQLGDLAIKRGVSRVWLLMRGPLKVKYFDVDLDWVGKFRNYKQAEFWTADTDEERFEMIMQARNGGSITPRYRKVLDAHVANGKISLHTHTKLESVSWDSSSKTWTCTTSSPDVALPSIDYIVFATGIQSDIRTIPFLQTLQQKHPIEYVGGLPCLNDDLMWNDDVPFFVTDSTSTTSQHPFENYTDENIMDTKEVVEAISSKALLRSGLIFRGQESGVDNNNIQLYISEISELTQSSDKEVEGLCGTCREYGLEYLHLLECPTTLPGKAKYDDSISPYRSASPAPGDWQPDGEAFRPVPSRDTWLQCFDTRSHFQDDCAVCELLISMLPKDSTGPWRVCWVGYGFYKMHDQHKSWGRKNADINWKTQRTPVIVVAPDQSADFEEWWAFRRVYGFVLPTRRRDEDPSIPRARYLNSHSVNFDAAKTWIRSCSGIHGDTCGSVGQSLSSLRVIDCRTKTVVPAQANCHYVALSYVWGTNHVTAPRDLSNWTLVPTLIQDAVVATIELGYDFLWVDFYCIPQDDAVEMDRQIRNMNKIYSEAQATIIAACSESAATGLSGVSSNVRQRSMTATVGQNTLAAIYSKEAESVWKSKWNTRGWTYQEGELSRRRLMFTDSGLFFQCAGASEGESCASESLDVSFGTQGLRSTTNDPWVFSDTRSVTKLPGMVWACLRAYTEKTLSFDTDALNAFAGILSRFAETSETFDHIWGVPVYQKLLHHPTHDGGNIEFIRQYKVRQTYSEQLAASLLWRPAKANYYATYRADFPTWSWANDTGTITCPDDNIRNLDLDISVELENKEKMHTETFFSLPAEQRPKVGKGLYVNAPTTYIHIETPVRDEACIYSATSFERQDALDLRHRGLLSLFRWGKDTKLGRHLAIIIGASQNTMSQRLTYHLLVLQEKEDHYERAFVLEVNDATLEQAVPEETWAGWKEEACEVLKLDKLPWIWANYPADLGFTEEKREDWEKLFLISKHLRVEKRVVLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNU9</accession>
    <name>A0A9W9CNU9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Probable aspartate--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="4">6.1.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">KOG1</name>
        <name evidence="18" type="ORF">N0V83_002600</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>tRNA(Asp) + L-aspartate + ATP = L-aspartyl-tRNA(Asp) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9660"/>
            <dbReference type="Rhea" id="RHEA-COMP:9678"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat RAPTOR family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.12" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10262360at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030307">
        <property type="term" value="P:positive regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04320">
        <property type="entry name" value="AspRS_cyto_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000038">
        <property type="entry name" value="Aspartate--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000278">
        <property type="entry name" value="WD repeat-containing protein mip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004083">
        <property type="entry name" value="Raptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029347">
        <property type="entry name" value="Raptor_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003483">
        <property type="entry name" value="PRK05159.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848:SF16">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14538">
        <property type="entry name" value="Raptor_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01547">
        <property type="entry name" value="YEAST176DUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM01302">
        <property type="entry name" value="Raptor_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="1242"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="17">
        <location>
            <begin position="1601"/>
            <end position="1912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="872"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="932"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1554"/>
            <end position="1577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="11"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="890"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="932"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="959"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1566"/>
            <end position="1575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00070516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4373861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1912" mass="215060" checksum="52CBBBDDC70A0B91" modified="2023-11-08" version="1">MNGATAPPPQEAQQLPQFAASSRPSTSAAANASPPSADMEEQLTTVRSASNGVQAARPGLLRARSDVGPRHPMLPSESAEDGSVDGQFKIRHGWDDQLNSEEYSNLLTSNFFMYYTDKKHETGGHPKSEDGVFPVQEWRMRDRLKTVSAVLALCLNIGVDPPDVIKTNPCAKEECWIDPTVTSQTPGHTPMNQIGKALQTQYEQLSMRTRYKLLLDPTTEETRKYTSTLRRNARNERVLFHYNGHGVPKPTSSGEIWVFNRNYTQYIPLSLYDLQSWIGAPSLFVYDCSDAGLIISNFNRFAEKHQTEFEEARRRDPSVEYVDFSDCIHLAACREKESLPTNPELPADIFTSCLTDPITMAVRFFILQNPLPSKVSLKDAHNIPGKVSERRTPIGELNWIFTAITDTIAWNSLSKPLFKKLFRQDLMVAALFRNYLLAQRIMRAYHCHPVSHPEIPQTHDHPLWRSWDLAVELILAQLPDLQAEARGEKDYIYKHSEFFSEQLTAFEVYLTQGAVEQKIPEQLPIVLQVLLSQVHRLRALILLSSFLDHGPWAVNLALSIGIFPYVLKLLQSQANELKPVMVFIWARILAVDQSCQADLLKDNGYQYFINIFNPNSGIPIQNASEHRAMCAFIISMFCKDYNQGQRASLSAELVESCLDHLKDMQNPLLRQWSCLCLSKLWINYEEAKWMGIRCMAHERLCELVVDPVAEVRAAMLHALTSFLGIRDVTAQVANIEETIASMILVMTMDGNSMVRRELLIFFSMFIARYKTRFIVAAFEHFSEEGNSALVTQSDERNGEGLFMRMRSTANGVSTGSSSCSQNTIYSAIWKEMLVMSVDPHPEIAKDAGIVVDYVLIALIESSLAKFVQPQLDEVPHNAPPSRPMRRAQEEISTPQKNSNPPTPTKDSSYLSLFGGLRKSTSVGASLKSLWTGSDVSQKSPGRTKTEDLYPSTPDSRPQTPEKYHIEEEPKPRGFKSRSLAEKPEIPLRSVFFEWSVEYFREPQMKPTEADEPGSTDYNGRLWRRNRNDKIIAETQPLKEIAVSNRWDVPRGYFDNLSQPQKMCFHQFEDHLVVTDNRDTVNIIDYSKAVSRINCFSNGNPLDSRITEVRFINEDDQALLMTGSSDGVIKIFRNYDRKGETELVTGFRALTDLVPSNKNAGLVFDWQQGRGLVLVAGDVKVIRVWNAGTEVCTNDLPARSGSCITSLTSDQVEGDVFVAGFGDGAIRVYDQRQKPATAMVKVWKEHKQWVTNVHLQRGGQRELVSGCRSGEVKLWDIRMDRSVKTIQATTDHLRTLSVHEHAPVFATGTQKHRVKIFNINNGQPVSYFEPYSGFLRDRSAPISATAFHPHRLMIAAAALHDNHVNIFSCHEPRHSAVKTVKLWEGSTAASNRAEKKAAAKARELAERTKKDAADAADVSADAYGELPLCGSKDFKPTGTPRADLADIASNEDKEITFRCWVGNARVQSAKLAFLNLRQGIMTVQAVVAASDRLSKQMVKFCGSVSTESTLVVTGLVKRVQEPIKSATIKDFELHIQKLFVEVKADVPLPLQVDDAERPLPSDAAAEEEQKQEEGSERPLVSLNTRLNNRTLDLRAKINQSIFVIKSGVCALFQEFLSKKGFVLVHTPKILGAASEGGANVFEVKYFDRPAYLAQSPQFAKQMLIASRFQKVMEIGPVFRAENSNTARHLTEFTGLDLEMEFQENYHEVMGVLEDLMLFIFGELNARYKKETELVRGVYHIDDFKLPESGKVPRIPFQEGIKMLREAGEELGDYDDLTTPQEKHLGRLVLEKYNSDFYTLDQFPLAIRPAYTMPSPADPALSNSYDMFMRGQEICSGAQRIHNSALLAEQMRKHDPPIDPNGPGTKDYVDCFKYGCPPHGGGGFGLERIVQFWLGLPNIRMCSLFPRDPQRVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNY5</accession>
    <name>A0A9W9CNY5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7730 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002554</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNY5"/>
    <dbReference type="OrthoDB" id="3798004at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056632">
        <property type="entry name" value="DUF7730"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38790:SF4">
        <property type="entry name" value="2EXR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38790">
        <property type="entry name" value="2EXR DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24864">
        <property type="entry name" value="DUF7730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7730" evidence="2">
        <location>
            <begin position="48"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="422" mass="47499" checksum="2E8A9650E2E447DC" modified="2023-11-08" version="1">MLLPAILFKPTKLSPLLTSGNGKDGEISPPARERKPRVLGEWSGKVDDQSQCAFLIKLPAEIRNEIYEYVFAEPPGASDFKIMEEEKKKEKDQQPREQEDEEEEDSDATTPPPSPPPPPPPPLQPSPSPSHPLSLLLTCRKLNTEATLLAYRLHPFRIEAPDMPFYKLHNRTLILPRPLFQAISRVSMSLGENKHASPIWAPAVQTFISHMILLSPNIGELVVRFRTPVGYRRDDGHFVGSVRPPSPSPPSSSSRGRGEHDVVEVLSCREHVPEWLSVAFAGVARDPYNFWRKSEKWAVFWPDVAHPEIHNDDDGGNDEEEMHRRWDACLAVLKQEGTGRKVRVRLRYEGQDEEDTRKPQRKGLGVTLVPGIPAEEDMLDLEAVTGRDGLEGLVYDPGEEYWEGLRAMKIERGEGGRGSDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNZ2</accession>
    <name>A0A9W9CNZ2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Rho GTPase protein rac1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RAC1</name>
        <name evidence="6" type="ORF">N0V83_002584</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNZ2"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000088">
        <property type="entry name" value="Ras-related C3 botulinum toxin substrate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0636">Prenylation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4373845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="200" mass="22117" checksum="DC6356DF7E940CF3" modified="2023-11-08" version="1">MAAPATQSLKCVVTGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPISLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSIVSPPSFDNVKAKWYPEIDHHAPGVPIILVGTKLDLRDDEATKESLRQKKMAPIQYEQAVMVAKEIKAQKYLECSALTQRNLKSVFDEAIRAVLSPRPQQTTKKGKSKCTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPM8</accession>
    <name>A0A9W9CPM8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Filamentous Growth Regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">FGR2</name>
        <name evidence="9" type="ORF">N0V83_002572</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPM8"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000171">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF4">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="156"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="434"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="467"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="496"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="66"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4373833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="525" mass="57960" checksum="31960437ADBD890D" modified="2023-11-08" version="1">MDHDVTNKDHENKVQEKDVENQHVADDVTSIGHGDILQLEHTDPVLNAKMHLINDAIDEIGFTRYQWRLFVLNGFGYAVDSLILLIQSIIAGQAALEFKPGYHNGLTIAAYVGMLIGALFWGLTADIIGRKIAFNVSLIICSIFAIVAGASPNWEVLGLFVCLSAFGGGGNLVLDTAVFLEFLPSYKQWMLTLMAAWWGIGQLIAGVFAWAFIPNYSCQDPSVYPDAPPCTKSNNQGWRYVWYASGSLVFVMSILRITVIRLKETPKFLVGEGRDAECVETLQFIANKYNRPCSLTLERLQECGTVDKVSRNNKGKLGFGEVWLHLKGLYSTKRIGISTTLIWFSWTLIGLAYPLYNVFLPAYLSSRGAAFGQPSPYTTWRNYTLANFSGIWGPVLAGWMCGTKLGRKYTMVIGALVTMAFFFAYTQVRTATQNVAFTCIINFCLNVYYGTLYAYTPEVLPSAHRGTGNGIAIGWNRIMGILSAVIATVANTGTPVPIYICAALYILMAAVAIAFPFEPYGKRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPS9</accession>
    <name>A0A9W9CPS9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">N0V83_002270</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPS9"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000016">
        <property type="entry name" value="Solute carrier family 25 member 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 15" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="29"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="126"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="231"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ4375186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="330" mass="36115" checksum="C88BDD8B70B5E4B9" modified="2023-11-08" version="1">MASESEKGANPFGPPDHTRLQRFRQVLAQPVLASFVAGGVAGAVSRTVVSPLERLKILFQVQSVGRNEYKMSVPKALAKMWREEGWRGFMAGNGTNCIRIVPYSAVQFSAYNVYKRFFESEPGAPLGPYQRLLCGGLAGITSVTFTYPLDIVRTRLSIQSASFATLKKEAGQKLPGMWTLLVTMYKTEGGLPALYRGIIPTIAGVAPYVGLNFMVYEMARTQFTREGQKDPSAIGKLGAGAVSGAVAQTITYPFDVLRRRFQVNTMSGMGYHYAGVGDAIRSIVKTEGFRGLYKGIVPNLLKVAPSMASSWLSFEMTRDMLMGKWNSGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQ54</accession>
    <name>A0A9W9CQ54_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQ54"/>
    <dbReference type="OrthoDB" id="4842781at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4375907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="275" mass="29987" checksum="0027D578247C5F42" modified="2023-11-08" version="1">MPISGGQVYDIENYIEKLRRGISERAPNPAGVAYVPYYDLDGNYGKSMDKWYKDFPTDDFGNDISFSSEEGSESSVTNSGHKVENVDASLRFGWLRISVSSTNEKSWSDTKALSNNAKVTIKLSCKQKQVFSVNPGKTWSVECTSTSQAPKLTLLRDVPAYTQLFPNTKDGLVPAAKNLVQTTGMLCVSGLRLSAQFDSSAKEVFDTEFKEAKNNNGALSFFGISIDANSSPDASKKQTHSASWDKNTGTLSFEPTSYVGNCTLLAMIGSKSNIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CRA7</accession>
    <name>A0A9W9CRA7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CRA7"/>
    <dbReference type="OrthoDB" id="3791982at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4377795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="271" mass="29525" checksum="0E09898E14B21BDA" modified="2023-11-08" version="1">MSSSSSSNGANQPAKSDPWADFEKWMNVNYPVAVNTPTPPDIPVILRVRKQAETTLAQKGTDSAPAGDAILGPDHSVRPTHVSPTPVTTSPEPAPTSPLNVPEGIPIVAYFRPQLLKGPGIDVVVGPNDASSSTHERESWSLPKALIGFYSPLSKSRLRSQVPGARKKFAPGSLLRRFFFDLVATFFHVPARVVGTTEEWDEIMSEHAELRANLLSGFRKGADQRQSLEAKDAYMVRHKTARPSSNVANMNENTIIPAKRRADGARVKKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CS30</accession>
    <name>A0A9W9CS30_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Calpain catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_000537</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CS30"/>
    <dbReference type="OrthoDB" id="424753at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000072">
        <property type="entry name" value="Cysteine proteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF379">
        <property type="entry name" value="CALPAIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="8">
        <location>
            <begin position="139"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="571"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="734"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="968"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="586"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="608"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="649"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="808"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="880"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="968"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4377708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1049" mass="118658" checksum="CD5BEB975700B716" modified="2023-11-08" version="1">MAGEAAPAPIAPRPRLPVRKQAPQRNIDEFWNKFTTKHPGKIFTILPDNLYAKRAAAHAPQGSIPGVNALASYEEAVESCSRKVAKIVKECRRLNQKYRDPHFDIEADFKRSQAYQDVPSDCLTGLNEEQTDFHPLSVKRVEDIFDSPRFFIEGATANDVRQGNDGDCWFMSALATISNKEELIQRVCVARDEQVGVYGFVFHRDGEWISEVIDDKLYLIKEDFDEAKLERYNWLELQNRKSPEEEYRTVMQTGSRALYFAQCSDPNETWLPLLEKAFAKAHGDYSAIDGGFVGEGIEDLTGGVTSEVFATDILDKDKFWREELMNVNKTFLFGCGQMGGIYGERKGIQENHAYSIMEAREVDGQRLLKLRNPWGRTEWTGRWSDGSEEWTPEWMQKLNHKFGDDGVFWISYKDLLRHYQHFDRTRLFGPEWTVSQQWTSVNVPWSVDYLDTKFKISLANSGPVVIVLSQLDDRYYQGLEGQYEFNLQFRLHKDDEEEYIVRSNGTYYMKRSVSTELDLEAGNYTVLLKIKATRMPDNPTPDEVIRATCTTRREKLLAIGLSYDLAHAKGQFREQEKEKAQRHRDHKIERKKAEMKQSHERRKRLRRKAELREQKKAAKETRRLNRAGDMSEVEKNLQSLSGLGISTEEENRASGDDEAKPMHQVVSRHNRASSTGAIRERSSSPSGGFQGRGGYNRGRDGYNAALPGVLQGRGGYNREPHGSATPEVVALQGRGGYNQSPLASPSLHGQGFQGRGGYNQNMPGELHGRGGYNQNMPGELQGRDGYNGRDGYNGRDGYNGRDGYNEGQTTESPTPTPQTDEFRPGTPSQRPYSPRPERLSPAPSRRATLTPIPGIRPGIQVTRGRGTSVCSHRAHHGMNSSDDVSDVSSWDSEVDGPCSLDSFSDDDDVPRHFSDRHNLRYVWYNGTRYPYIEDEDEFERDPWNAVCVVGLRVFSKDNTDVTIEVHNGEGERKKRSDGDDASIASMGTVRRKEKELDVDDSAADATSPLRTRTTGLDDELKNRAKEQALVSHVGTAPAGQEPNRQGTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY54</accession>
    <name>A0A9W8XY54_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_011008</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4362066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY54"/>
    <dbReference type="OrthoDB" id="9976870at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF15">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="38"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="335"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="335"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4362066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="378" mass="41880" checksum="35E7BCA4A8127E7B" modified="2023-11-08" version="1">MSTAVDPNARIISPNGLPLAILVLSAVFLGLSLVTVALRTYTRLAKGIFGWDDGFIVIGTVVYTAATGLAIYAVLVGLGRLEIELNAWQWSEAMKYYIIWILTYVLALATVKSSVCITIMRIASTKMNLRITVYVLLAITWASFFITFVGTLLYCRPVETIWTPTLILSGKGTCAPVDTFVIIGHVATVSTIVTDMALVVVPAIILWNTQMKRQAKLQAFGLLSFASVASIITMIRIPYINRFKEGKNLQFWVAHIMLCSNIETGIGCIASSVPSLRHFFRRDNTESGSGGVTSKGARNFFTVGSEPRERKIRDSFRNPTDLGFSLSTVHHGRAEESWERLRDRDDDGSDAPMDPKGIHKERTYAVDIEMNDIEKNTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XY76</accession>
    <name>A0A9W8XY76_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">R3H-associated N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010101</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4362984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XY76"/>
    <dbReference type="OrthoDB" id="10256743at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025952">
        <property type="entry name" value="R3H-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13902">
        <property type="entry name" value="R3H-assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="R3H-associated N-terminal" evidence="1">
        <location>
            <begin position="79"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="332" mass="37227" checksum="F13F171E2D4581EB" modified="2023-11-08" version="1">MAIGETPSSPQHAAPPQPIDIEAWTEQATVALSSVTISAPGDVVEGTSVTLAIPLDEHDAPARPATASAAKEGGYYRRKEPLRRDSLKRREALLKGKEGSRRRQRWENDRLLNNPYAEPPLPRDWEVHPTHRVTNVPYYLAPLWDAGLKRQSNERKTAAVAQKAATKTVANKPTTPGVVPKELRDKLKRSRGAKGLLMDLESEVRKFVADWEEKERKAEQEGLPLDPDSDDDEIVFVGRNGQMNDMQESKDAQQIKRELLLFESPEEDMGGSFGRWLVHHIGVYYGLSTWSITTGNPARREAYIGLKDTKLRTGNRDQQICQPLPRPLYGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYD4</accession>
    <name>A0A9W8XYD4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterokaryon incompatibility domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYD4"/>
    <dbReference type="OrthoDB" id="2157530at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052895">
        <property type="entry name" value="HetReg/Transcr_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24148:SF64">
        <property type="entry name" value="HETEROKARYON INCOMPATIBILITY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="13"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="574" mass="64411" checksum="906CBACDB4A99B91" modified="2023-11-08" version="1">MRLEKVKESTLDFQALSYAWGDDEPSYGLLLSDISRSNEGRPGKDTIEGTIYPMDYRFFSVRSNLYQALKRIRRSESCSWIWVDALCIDQTNESETSQQIPMMPDIYYNAWNVIAWLGEEEDGMKEGVDMVPNILNLRILDYALRGEVLDEDILRSWGPFAKLLRRPWFERRWVIQEVAGARRPSVRIGEHILSWVDFADAVELYSDKIDLVQELLSRSYHSHAIRKLGKTESFRAMALIDLSRKVFRKSSDCRVLARLMTLESLVLTTSSFAASQPKDVVYALLYLAHDTSSRRSLTKLNERGDLLSSNLITDKKQPSTVKEYLERVQVATENRQIFRCEAVCPPFNPSAVQQEDETVIGMGLKHIKEGPVVVSLQHCPDIMSPYKDGLHPAIDPAVRQRPSPKANVKVQAYKLLSSDKHTLLYASTDTSERNDGAANNCTLMRPLVTVSSAAAKPTTKANRVVVKGKLRIVAPATFSIQQVEVEEREVESWVGIADVDSHRSQVQDQDSAGRKSGWTAPVTTDVRAWGASGRFCWLASPRTTMHCLVMLAASGIGAVGHVRALLTSTGTSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYJ4</accession>
    <name>A0A9W8XYJ4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_010451</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYJ4"/>
    <dbReference type="OrthoDB" id="2585655at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:001500">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF20">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G13880)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="301"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="349"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="387"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="414"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="481"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="66"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4362358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="521" mass="56627" checksum="77958C66DBCCF6B4" modified="2023-11-08" version="1">MGFGVLEDRYLAAPPGTATINTSGPAEDSTIDSSSLKKDGNIILQPQPSDSPNDPLNWSRIRKETIMVILAFASGVTTALGPMVTPGLPLVAMKYNVSIDLVASLIIGFLAFWIGFTTFFTAAGANIWGKRPFFVISTVLLLATNVWGYFTTSFVSLAVMRVVQGMASAPLETLVTSTVSDMYFVHQRGTRISIWGCMLASGVLLGQTIAGAIITNISFEATFGISALVFIPILCGIYFFVVETTYYGARPTGKKLAPEEKDMSFLDDDEEKDTYRQQLRIFRGRMSAESFWKNAWKPVPLIAYPAVLFSTIVYGSYFTWLLTISVLSVSVFTAPPYNLNPAQVGLTNLPLLVVSLIGAPLSGWLADAIAKLMARRNNGIFEPEFRLTLMVPATLFATIGFLGFGMSTSMGAPLVWPVVFMSIHSLSVPFASTASLTYVIDCHPKDANQAFVTINFTKAVFTFAATTYANGWMAQVGPRGVFDAITVVNLCVCALTVPAYVFGKRFRSYVARSAFGQRLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZ31</accession>
    <name>A0A9W8XZ31_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bacteriophage T5 Orf172 DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_010141</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZ31"/>
    <dbReference type="OrthoDB" id="3511049at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018306">
        <property type="entry name" value="Phage_T5_Orf172_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10544">
        <property type="entry name" value="T5orf172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacteriophage T5 Orf172 DNA-binding" evidence="2">
        <location>
            <begin position="248"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4363023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="445" mass="50978" checksum="D8CD0539DC2AC38C" modified="2023-11-08" version="1">MLQIKANLLPGRFPSPTPSRETTPFEVHTPLLPGIPDIARQVPQADVPSRPASSDFTAASQFRNRPQFKEAQSEPTCLPTMSALLPSDRASPLASPCNPYRTAKDRRRVSDPLLHSRSGRTVRNDPSAIRKTRQKRQRLSDTPQYYSCPTTPSQSSTTSQPPSTPPRRNTSLTESHNNINRPSPREGRVDNGQHTERKSESEQNRRGTPEKGRSRSSASPKTPVTPRDIHAKIRSALLEDLTFSEKKGIIYILHDPDRPELGYKIGATTRSTYLDRINEHRQKCNFEPNVVHEGIEIDYCFRTEKLIQAELDNFCQHWDCTGTHKSPRVSKSETHEEYFLVSEELAIQTAKKWEDFMRREKPYGWSGKLKPVWRYLLEKRRCTFIADDPSTKDFLAVREHWNTVMRAPPETADYVEFYWRVTNTTCANFLPSSSLILSFQAVRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZI7</accession>
    <name>A0A9W8XZI7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_010258</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000020">
        <property type="protein sequence ID" value="KAJ4363138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZI7"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045231">
        <property type="entry name" value="Yip1/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN YIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236:SF1">
        <property type="entry name" value="PROTEIN YIPF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="8">
        <location>
            <begin position="156"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4363138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="294" mass="31564" checksum="C534CFAE6880A7A4" modified="2023-11-08" version="1">MAAMMPSAATSSNPYDNDAVERDLIDPDDATLDDLDDPLQGTSDRAPLTGNIQRGSANTQSYLTSSIPGEDRRAPTNTIDETVWETLSRDIFAIWEKMKQVLYPKYLLGGMMQRGGGIGAAERGEASGFGGGLRDIAGRWPDADVILQGGMSEGLRDWDLWGPLIFCLLLSLFLSWGARGTQKDLVFSGVFAMVWIGEAVVTLQIKLLGGNIAFFQSVSIIGYTLFPLVIASILSAVGIPTIVRVPVYLVLIAWSLAAGVSIMGGSGVVRNRVGIAVYPLFVFYVALGCLCFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZL0</accession>
    <name>A0A9W8XZL0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZL0"/>
    <dbReference type="OrthoDB" id="3941538at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="636"/>
            <end position="659"/>
        </location>
    </feature>
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        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
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            <begin position="93"/>
            <end position="106"/>
        </location>
    </feature>
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        <location>
            <begin position="107"/>
            <end position="141"/>
        </location>
    </feature>
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        <location>
            <begin position="142"/>
            <end position="155"/>
        </location>
    </feature>
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            <begin position="156"/>
            <end position="171"/>
        </location>
    </feature>
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        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
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            <begin position="192"/>
            <end position="222"/>
        </location>
    </feature>
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            <begin position="260"/>
            <end position="276"/>
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            <begin position="277"/>
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            <begin position="295"/>
            <end position="324"/>
        </location>
    </feature>
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            <begin position="342"/>
            <end position="351"/>
        </location>
    </feature>
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            <begin position="352"/>
            <end position="361"/>
        </location>
    </feature>
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            <begin position="362"/>
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        <location>
            <begin position="640"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="659" mass="72078" checksum="113CE09D46C2ECBA" modified="2023-11-08" version="1">MPPPGPYVPPNQGTSAQSYNSQQQPGFQQSPAPSPYQPATSGYGAPSHSHKAGGTFGQMVNQAVTTGKPMLNKLGKTISSKLGNKPPAGPPQHLQSYQTYQNHLKPSQSYQQQQSQTYSPQPQAQSTFTPPQQSPYPQSSYVTPASGHSGQSNYFPQQPAQTPNAQPQAPNVSTYNAPQFEQSGFTTNQYAQGQQGQQGQSGQGQTLPAQPQAPFQQDQHLQNQYTGQQIGIVGGLQNTQQSQNIQFSHVSNVSPDIPAQTAQSQWNYSQPASEQLQPADPQQQPTNPPNLTQAFQSGPSQSSPIQHDQQQWNNISAGTPQDQRPGQLPPHSVSPPPLHQVNIQPQVSANKPPQPSQPPTPASQQAVPQSAPTEFVAELPADMSNLSLMESKGQQSAQSPQYQAYTPSRPQSMSPAPGFTIPRRSVSASNFPLADPWRFVDSITELPTREFYILADLLFDALDRKFEPRNTGLLEAPKILGSWVKLTEDAHELFSYKSYAALAKMWSLEGIPHIMVPCQLALCPIWNFHQQYHALELKVSADPPTPISTYATYMPALNRPAWYKFFFLEVMQAPEDIGDLIPLLCAETYRPGVLNHPDLNKRDKGVIPALQARASAIQTYAIGRVCEETKAAMSVDPDLSSAHQTHSAAQQQDPQGIWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZQ8</accession>
    <name>A0A9W8XZQ8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_009410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZQ8"/>
    <dbReference type="OrthoDB" id="3800314at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4363957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="550" mass="58931" checksum="70FEB510C85A8D57" modified="2023-11-08" version="1">MVARCDPRWVEAELIPFLNIAFRPRPRGVWENMIRSAEENIHDIRFPDNFPSTIDSIRLALINPELLLDWEVKTFKRVTPNQSLCISIIVRHALTFGHVPIFSLGTEENPEFPREALRAAIAEANAMNTQDPNITAAVVNVKTGETDVDKTDVSIAEVDNAETDTMKTDDAKIIPVEVVDATTGEVEVDMTDVSNAEADNAEAGPVKTEDAKITVAEGVEAMAGEEVDNTDGSNAVTDNAEACTVNDNNVEAFADVDGPTQRHLLAHGIDAGYSSVGAQKPQGRKKGKGNRRGNRSNGMGARGFGRGTGPVPPQLAYETPQGRPTHVGRSYGGPPGHAAGFVERPNGFYGVQSLQTAEPERTPGYTQSNLPYQQGDHSDTYPHFNGYANGYAMPNAYNVPQVEPFAGYYGMQGYPNSWSVGSAPPGVYQGASDRFEPSHSTVAPNGFQPYVAPPQFGPSSAVVAPNGFQYAAPLQFESSRPAVAPYGFQPSVASLQSQMSPAAVAPNGFQRPVASLQSEASMFQPGTGPHYLQGDAEDLVPTLEGGISRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XZU9</accession>
    <name>A0A9W8XZU9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ARM repeat superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_009745</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XZU9"/>
    <dbReference type="OrthoDB" id="49511at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052587">
        <property type="entry name" value="TELO2-interacting_protein_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057567">
        <property type="entry name" value="TPR_TTI1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057566">
        <property type="entry name" value="TPR_TTI1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049362">
        <property type="entry name" value="TTI1_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460">
        <property type="entry name" value="TEL2 INTERACTING PROTEIN 1 TTI1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460:SF3">
        <property type="entry name" value="TELO2-INTERACTING PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24181">
        <property type="entry name" value="TPR_TTI1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24173">
        <property type="entry name" value="TPR_TTI1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21547">
        <property type="entry name" value="TTI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TTI1 N-terminal TPR" evidence="2">
        <location>
            <begin position="7"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="TTI1 C-terminal TPR" evidence="3">
        <location>
            <begin position="762"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="751"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="794"/>
            <end position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4363452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1054" mass="116746" checksum="ED723C037136FBBB" modified="2023-11-08" version="1">MERQQAFQRLKQPCIQLLQVTARLAQDPGARKDLIQALTNLLSTLQAITTTSNVLDAKLTEYAFVPISQVLRISRQVPVRALELCLQCISVLLCAGWGGGLDPALSGQLLILFTFLAKPSSAENGIAATSEELQSLALQCVGNLLTEVARTEKGKQAMTATANIPALGETVLVMVDSLTNSNSNGVRLKAVTALAALNTALADDDALASFLPKIVSSLTKVLTSNISNRPGFRVVEQSLKLLSTLLLRLLSDQNTRGLPKTGPSEQSKNQTTITRSISWLQATAAQIKIALANVFKLRDHDKLEIQQALLQLCLCIIQDCRTSLNDCTSMAIETIINLVGHEGFQSTIENKLKGLLSADQNLSDLLRESLHGWIVALPRLMQSKDDRSRRRIINQISVTLRLFEQDSTTIDERLADGLRDGVSTVFSDSKGLEELSEKTSEFTLDRALVLGSVKTPSFQQLQLRFKGQDDTMAEFKLLLQELAKSDSALAVAQELTRTIDVGTLETQLASFWVSVNLIREITTNSPSFDDLFDLGTPNIREEILDTLYSHSLAILNGQAPITDTPWHFYALALETVALQATRYKSEFRPELSEVLYPVLHHLGSANASLREHALTCLNIVSESSGYSNAGELVVANVDYIVNAVGLKLAIGDVSPQAPQVLLMMMRLCGPSLLPYMDDLVGSIFDALERYHGYPKLAELLFSVLKGMAEEGVKAPQLALTTGNEIKTSRGQKKVVTMAHVIEALKRVEVESRKREEENKNWSAEPVPKEPWKDSNTTTDEDQNDPTGDGRNPPDAQPEEPPPPAPRTFDILLKISELTQHYLTTTSPSLRNSLLSLLRTTIPALAKHENSFLPLIHTLWPVLLPRLQDPEGYIVSNTLDVVGLMCEHAGDFMRTRIDDAWDIIRKVHRRTIQQTDNRSKSSKPLSTSLNISALETNMTKLSMGPDSTSEMFRPEMYVDAPTRMIWKSLVHLLCAIAKHVGIREERFDEILDMLDPILEKSAEVREALEEYNADAVWLRLYKKSKLRTETTKPVDSFVSIGRTPVGKPHWHFVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y049</accession>
    <name>A0A9W8Y049_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y049"/>
    <dbReference type="OrthoDB" id="2997776at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4364430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="200" mass="22395" checksum="9595C0EF7C6A6E0A" modified="2023-11-08" version="1">MPNYTITIGGGGGGNSFLLLVNDTALPGLDININIAERGAEIRFKWREMLHLFFREHARQQMLRQKWHLEATLKMAENNKGEKLLLPSDYHPSPPWCVAAEAQIRKEVRRKRLAEAYQGNEEMSWAIASLKHLEQDAVGAAAAGGHCSKKAVTLDAEIPGAGLGERWFGRSNLVQELFLDEWSCLHRIETKIEHLSGGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0D6</accession>
    <name>A0A9W8Y0D6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_009490</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0D6"/>
    <dbReference type="OrthoDB" id="3162524at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF697">
        <property type="entry name" value="AMINO ACID TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="358"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="405"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="93"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4364035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="449" mass="48155" checksum="47ED6606F421995F" modified="2023-11-08" version="1">MSDEKKDIQPIASSPARSIQAGEVEYAKPADDAFEVFKTHTDGVQFRLVGWLRASVIFLKIIFATGVLSIPTAMYGLGAVGGALSVIGWGALNTYMAQVVGGPILREICGGLFIIAYVLCSGSGIVGLSVGFNALSEHSACTVWWAFLAFVLVVVCASVRKFEKIGWLSWAGFISVYVAVFIVVVGVTTRDRPAAAPQTGDFDFGYHAIAYPTFMVGMTASCTIFVSSAGTSAFLPVISEMKNPREYNKAVYVCMALVQSSYLTFALVVYKWCGKWVTDPSLGSAGTTLKKASYGVGLIGLMVSGCLYLHVAAKYCFVRILRNSQHLQKNTIIHWGTWLGCTIGLGCLAFILAEAIPIFSFLIALTGSVCFAPLAIMLPGWLWLHDHGDYKRGGPLKMMAYYAHWLLILIGAFTCVGGTYAVIKSIIGAYDSGLIGSAFSCADNSGTVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0I0</accession>
    <name>A0A9W8Y0I0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="3 7">2.7.7.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">UGP1</name>
        <name evidence="11" type="ORF">N0V83_009405</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a central role as a glucosyl donor in cellular metabolic pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>alpha-D-glucose 1-phosphate + UTP + H(+) = UDP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="3 7"/>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0I0"/>
    <dbReference type="OrthoDB" id="932129at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-alpha-D-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00897">
        <property type="entry name" value="UGPase_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000001">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000002">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002618">
        <property type="entry name" value="UDPGP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016267">
        <property type="entry name" value="UDPGP_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43511">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01704">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000806">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 11" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000806-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000806-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4363952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="523" mass="57855" checksum="69A302F78DAF101B" modified="2023-11-08" version="1">MASRAIPSHLKPSAAAGSDEGGFSSQRHHGKSQSHVAFENTSTNVAASQMRNALNKLADTVTDPAEKKYFETEMDNFFALFRRYLNDKAKGTAIDWNRIAPPKAEQVVAYDDLANSEAVEYLNKLAVVKLNGGLGTSMGCVGPKSVIEVRDGMSFLDLSVRQIEYLNRTYDVNVPFVLMNSFNTDTDTASIIKKYEGHNIDILTFNQSRYPRILKDSLLPAPKENESDIANWYPPGHGDVFESLYNTGMLDKLLDRGIEYIFLSNADNLGAVVDLRILQHMVDSKAEYIMELTDKTKADVKGGTIIDYEGSVRLLEIAQVPKEHVNEFKSIKKFKYFNTNNIWMNVRAIKRVVEGNELAMEIIPNGKSIPADKKGEADISVLQLETAVGAAIKHFKNAHGVNVPRRRFLPVKTCSDLMLVKSDLYTLQHGQLVIDPNRFGPAPLIKLGSDFKKVSSFQSRIPSIPKIVELDHLTITGPVNLGRGVTLKGTVIIVATEGSTIDIPPGSILENVVVQGSLRLLEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0Y4</accession>
    <name>A0A9W8Y0Y4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7492 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009633</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0Y4"/>
    <dbReference type="OrthoDB" id="64281at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055915">
        <property type="entry name" value="DUF7492"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24320">
        <property type="entry name" value="DUF7492"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040995960" description="DUF7492 domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7492" evidence="2">
        <location>
            <begin position="19"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="414" mass="43887" checksum="C401B34E169369BD" modified="2023-11-08" version="1">MRNILRTAVVGLAAAPLALGHTWIEQMRNVNDKGEYVGEYGYPRGMVGKTDPGFNGNSMNWELPGSNLQGNVFINESTPLCHPSQRKQVQSSDKYPRLQAVPGGFIAMRYMENGHVSLPNNQKGKPKKAGTVFIYGTTDPKEDETLATVLQWSQDGQGGNKGGKLLTMNDFDDGRCYEVNSSPISTQRQKSDPNFAMGQVVEGAPGNYPLFCESSVQLPKTADLGKPYTFYWVWQWNTVPGADPGLPAGKDEYYTTCIDVDVASANVALAADAESKFALGQQDAMTIAVSDFASRTALMTDVMKAEVGPIFSSKPTGAASSSTAGAPAATGGAPFLNTTISAAPTGIPTLTGRPGASSTLSLGNADVVTVTDTVYVTVTAAPEATQPAASAVTPRAAHIIRHKNGAKFRSMFTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y104</accession>
    <name>A0A9W8Y104_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_008980</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y104"/>
    <dbReference type="OrthoDB" id="5596414at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173">
        <property type="entry name" value="ANKYRIN REPEAT CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173:SF74">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="154"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="188"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="348"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="391"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="405"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4364387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="414" mass="45875" checksum="F23990C66012D354" modified="2023-11-08" version="1">MVAALLRRSDIDVNKRVLKGCSPIHHAARNGDTGLIRMLLACNRIDPNNLTRAKRQTALHIAASRGHLDIVKLLGNLIDINSGSSSKHTPLHLAAASGHTEVVKYLSSQDGIKINSEDRWQHTPLLLAAAGGHVEVAKYLINQDGVDVNNTSHRGLTLLHFAAQNGHTDMVKLLLDQNFTDPRCKDRGGNTPLHLSAFSGHWATVHVLLEHSKNHNISLNQSSGREECDRVEVVRRLLTHHDFENDNRLDFHWPHYSLLHIAAKKGEVDVIRFLLAHPNIDVNNKDKYGVSPLMVAAREGQLDAGRLLLEHKDIDINLRGPYGWPALESARVRGRKDFVDLLLSYGAKDDESNSSSSPREESNPANTTTLAEVHSDTPLEPGVEVQAFSFLDEDSDEESMDALNVNDTMDSMGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1I5</accession>
    <name>A0A9W8Y1I5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Elongation factor 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Eukaryotic elongation factor 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">TEF3</name>
        <name evidence="18" type="ORF">N0V83_009251</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1I5"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000076">
        <property type="entry name" value="Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.990:FF:000001">
        <property type="entry name" value="Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000193">
        <property type="entry name" value="Probable Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1390.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047038">
        <property type="entry name" value="eEF3_chromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040533">
        <property type="entry name" value="EF3_4HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047036">
        <property type="entry name" value="EF3_4HB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF5">
        <property type="entry name" value="ELONGATION FACTOR 3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17947">
        <property type="entry name" value="4HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24984">
        <property type="entry name" value="HEAT_EF3_GNC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24987">
        <property type="entry name" value="HEAT_EF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 18" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <feature type="repeat" description="HEAT" evidence="15">
        <location>
            <begin position="187"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="17">
        <location>
            <begin position="442"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="17">
        <location>
            <begin position="688"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1028"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1031"/>
            <end position="1053"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ4364654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1064" mass="117831" checksum="F4C1F8B2420304B3" modified="2023-11-08" version="1">MAPAALHDASAVKSENSKALKALEELMGKLTVSKAQDEINAAASSIATFINGDIEEADAPTKAVELLKKQLASKKDAVARERALDAIRAIAQHAQVSAAVEPYLVSLLPNVLAAVGDKMSGVKVAAQTAAEAIVAAANPNAVKAIIPHIIHSLESAQKWPEKMTDLKCIEILTKSATAQMAFRVPDLIPVISGAMWDTKPEVKKAAYATMETLCSLISNKDIERFIPELIKCIAKPENVPETIHLLGATTFVTDVHEPTLAIMVPLLERGLVERETAIKRKAAVIIDNMCKLVEDPQIVAAFLPRLMPALEKNHDNLADPEAREKTRQGLDTLIRVGHVENGKIPELEKVGDIATIAGNLKAVLPSKYKIDERFTPVVNYIGAIGGQLIDEKDYEPLNWIANAGPFVAVLVGDEESKEITEALRKRSLPGAAAENAVEPDEEEGEDLCNCTFNLAYGAKILLNQTHLRLKRGQRYGLLGPNGSGKTTLMRAINNEQVEGFPKQNEVKTVYVEHDLDSADTEETVIGWTQKKLKSVGIDKPQEEVEKTLNEFGFSEHMYSGPIGALSGGWKMKLALARAVFEKPDILLLDEPTNHLDVKNVKWLEDYLTSSPCTSIVISHDSKFLNNIIQHVIHYERFKLKRYRGNLDDFVKRVPTARSYHELSASDMEFKFPEPGFLEGVKTKAKAILRATNMSFQYEGTSKPQIADITFQCSLGSRIAVIGPNGAGKSTLVNVLTGELVPTSGEIYHHENIRIAYIKQHAFAHIDHHLDKTPSEYIQWRFQTGEDRETMDRANKIVTEEDEKAMDKIYKIEGSQRRVIGINSRRKFKNSYEYECSFALGENVGMKNEKWTPMMSADNAWIPRSEIMASHSKMVAEVDQKEALASGQFRPLVRKEIEAHCANFGLDAELVSHSRMRGLSGGQRVKVVLSACSWQRPHLIVLDEPTNYLDRDSLGALSKAIKTFEGGVIIITHSAEFTKDLTEEVWAVMDGRMTPSGHNWVQGQGAGPRLAKDDGEEVETFDAMGNKIASQAKKKKLTSSEMRKKKKDRMARRKRGEEVFSDEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1M3</accession>
    <name>A0A9W8Y1M3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DASH complex subunit DAM1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Outer kinetochore protein DAM1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">DAM1</name>
        <name evidence="17" type="ORF">N0V83_008254</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DAM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1M3"/>
    <dbReference type="OrthoDB" id="5586015at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990537">
        <property type="term" value="C:mitotic spindle polar microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990758">
        <property type="term" value="P:mitotic sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013962">
        <property type="entry name" value="DASH_Dam1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28113">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28113:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08653">
        <property type="entry name" value="DASH_Dam1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="155"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="185"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4365634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="228" mass="23975" checksum="64A71AADB341F026" modified="2023-11-08" version="1">MASTTPTHEPPAFRQRTRSKSPLSRSRPTTPLRASSRSSLRDSAQRGRGASASGGNALEGLQDGFAELSDAMADLEQNFMALQLMHESLARFSESFAGFLYGMNMNAFCVDFPEAPIAESFKRPQNHPDLGAANFNRSQGPEDMEATFLTTDTSFVDNPPSSKISSKFQTPKSPAPASKSMGAPRGRGGIPRAGGRGGGIPVRGGSTRGTRGGSGIARGAAGRGRGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y298</accession>
    <name>A0A9W8Y298_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cell wall protein PhiA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y298"/>
    <dbReference type="OrthoDB" id="4093325at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040847588" description="Cell wall protein PhiA" evidence="1">
        <location>
            <begin position="21"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="17925" checksum="C6C69CEAB7A1E37E" modified="2023-11-08" version="1">MRFTTTTAAIAASTAALTSASPCGSSSNTTIAAGDIFRILTIRSGSALQYGSLQASNRALYINTPSQNASCSSSTPTNYASFFLSTDGSLYLNTDGNPPQQLFVDRSGMGQGVIQYTTGAEQPGRNSERTGWAVDGNGDLTFKESGLQACPGRWGRVECLVGWERESGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2C1</accession>
    <name>A0A9W8Y2C1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008890</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2C1"/>
    <dbReference type="OrthoDB" id="548867at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005654">
        <property type="entry name" value="ATPase_AFG1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169:SF2">
        <property type="entry name" value="AFG1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169">
        <property type="entry name" value="ATPASE N2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="78"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="201"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="289"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="80"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="298"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="327" mass="35866" checksum="4C3106A719B99764" modified="2023-11-08" version="1">MARRFITLLDAMYEARCRLLITAVTGPDDLFFPASKASSPTASSSGLSETADEQGLLNDAVYPETYSEIYQDLNSPFRPNVSSYNTDGSSTGTGSLAADALEDDPPNRVRRPGSSFTDERRLHPGTPNFADIGGLTGEDEKFAVKRAESRIWEMCSGRWWDRHASSVSASSSDDGTTGSGPYSDNANANANTWWRPLPLSSRHWEHPTSTPPIPKTNPTIAADNKPQPQRQNALPPNVKILSREEIEQDKRKEAEDMFAHGASPFRTHPDAPPKFSWTHAWGMMTWGKKAGPWGKGVEGLEERKRDGEEKEEGEKGNDGSRGGKGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2H9</accession>
    <name>A0A9W8Y2H9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="8">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_008149</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the tannase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="8"/>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2H9"/>
    <dbReference type="OrthoDB" id="3039123at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030600">
        <property type="term" value="F:feruloyl esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011118">
        <property type="entry name" value="Tannase/feruloyl_esterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33938:SF7">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33938">
        <property type="entry name" value="FERULOYL ESTERASE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07519">
        <property type="entry name" value="Tannase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4365530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="582" mass="62031" checksum="4A2C93CF293EA912" modified="2023-11-08" version="1">MLAAGANAASLADVCTVSNVQAALPANGTLLGLNLLPSTVTASAVYNATIGGGIGATTTSSGTTYNYCNVTVSYTHTGKGDVIALKYGFPEPSTFTNRFYLAGGGGFTLSSSATGGLTYGAASGATGASYDAFSNAYDAKVLYGNGSINWDATYDFAYVALGELAKIGKPLTEAFYGLNGTKLYTYFEGCSDGGREAMSQVQRWGEEYDGVVAGAPAFRFAQQQVLHVYPAAVENINGYFPPPCALDKIVNATIAACDPLDGRTDGVISRSDLCKLNFNLSSLIGESYYCAAETSSSLGFGFSKRQAAGGAAGSQTSNKPEQNGTLTADDIAIAQAVYDGLHDSEGRRAYLSWQIGSGLDDADTTYNNNTASWELNIPSTGGEWVTKFVQLLDIDTLANLDGVTYDTLVDWMNTAMVRYLDSLQTTLPDLTPFQSSGAKLIHYHGESDPSVPAASSVHYWQSVRSIMYPDLSDDEAIEALSDWYQLYFIPGAAHCGVNSLQPGPYPQDNMATLIDWVENGIKPSRLNATVSSGDYSGEVQQLCQWPTRPLWSSNSSDFDCVNDEASIESWTYTFDAFKLPVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y372</accession>
    <name>A0A9W8Y372_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Nitrate reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">N0V83_009029</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 17">Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>nitrite + NADP(+) + H2O = nitrate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19061"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16301"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.7.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </cofactor>
        <text evidence="18">Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the nitrate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y372"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050464">
        <property type="term" value="F:nitrate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008482">
        <property type="term" value="F:sulfite oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006809">
        <property type="term" value="P:nitric oxide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.650:FF:000001">
        <property type="entry name" value="Nitrate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000016">
        <property type="entry name" value="Nitrate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000049">
        <property type="entry name" value="Nitrate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.420.10:FF:000005">
        <property type="entry name" value="Nitrate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.420.10">
        <property type="entry name" value="Oxidoreductase, molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005066">
        <property type="entry name" value="MoCF_OxRdtse_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008335">
        <property type="entry name" value="Mopterin_OxRdtase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012137">
        <property type="entry name" value="Nitr_rd_NADH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000572">
        <property type="entry name" value="OxRdtase_Mopterin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036374">
        <property type="entry name" value="OxRdtase_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022407">
        <property type="entry name" value="OxRdtase_Mopterin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19372:SF7">
        <property type="entry name" value="SULFITE OXIDASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19372">
        <property type="entry name" value="SULFITE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03404">
        <property type="entry name" value="Mo-co_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00174">
        <property type="entry name" value="Oxidored_molyb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000233">
        <property type="entry name" value="Nitr_rd_NADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00407">
        <property type="entry name" value="EUMOPTERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56524">
        <property type="entry name" value="Oxidoreductase molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00559">
        <property type="entry name" value="MOLYBDOPTERIN_EUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="9 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 18" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="14 17" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="20">
        <location>
            <begin position="536"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="21">
        <location>
            <begin position="638"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </ligand>
        <ligandPart>
            <name>Mo</name>
            <dbReference type="ChEBI" id="CHEBI:28685"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000233-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ4364435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="892" mass="100586" checksum="59CD263C9FFD1CBF" modified="2023-11-08" version="1">MSVAPQEFQSAPFPPPSEHQPQPHAASKSIQLPPTPPETVDGDDSKSTSSTNLEPDFPLPPPANTPPQVLDVDKATPDSHVPRDPRLIRLTGVHPFNVEAPLTDLFNEGFLTSPELFYVRNHGAVPEVHDEDCMDWEFSVEGMVANPIKITLRQILEEYENVTYPITLVCAGNRRKEQNVVRKSKGFSWGPAGVSTALFTGVVMKDVIERAKPLRKARYVCMEGADKLPNGYYGTSVKLNWVMDPNRGIMLAHKMNGETLRPDHGKPIRAVIPGQIGGRSVKWLKKLILTAEPSDNWYHIYDNRVLPTMVDPDESAKNPKWWMDDRYAIYDLSPNSAIAFPAHEEKLSLTSAPESYKVRGYAYSGGGRRITRCEVSLDKGKSWRLANIEYAEDKYRAYEDRELFGARLDMDWRETCFCWCFWNLDIAIAELQETSDILVRAMDEAMCIQPRDMYWSVLGMMNNPWFRIAIHKENDTLRFEHPTQPALLPGGWMERVKKAGGNLTNGHWGETIGGEEPDPAAIETPKEIKMTKDGVDRIIEIDELRKHDKPENPWFVVNGEVYDGTAFLEGHPGGAQSIISAAGLDASDEFMAIHSETAKAMMPDYHIGSLDEASRRILLEGEPTLESSEPRPVFLDSRVWNKTLLESKKNVSWDTRVFRFKLDYDEQTLGLPTGQHLMIRLRDPVTREAIIRSYTPISQTTQKGYCDVLIKIYADTTEKVGGKMTKALDSIPVGHWVDFKGPIGKFEYLGRGRCSVNGKEKKVKKFFMICGGSGITPIFQVLRAVMQDKEDATHCTVLNGNRLVEDILCRDDLDMFARENEDRCKLLYTLTQGPEDWTGLRGRIGEGLLKEHCGLADDGESLVLLCGPEALEKSSHKILNEQGWKDEDLLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y379</accession>
    <name>A0A9W8Y379_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Folylpolyglutamate synthase</fullName>
            <ecNumber evidence="10">6.3.2.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">FOL3</name>
        <name evidence="10" type="ORF">N0V83_008923</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.12" evidence="10"/>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y379"/>
    <dbReference type="OrthoDB" id="5212574at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008841">
        <property type="term" value="F:dihydrofolate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF0">
        <property type="entry name" value="DIHYDROFOLATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01012">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur ligase central" evidence="9">
        <location>
            <begin position="73"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="486"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="551"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="610"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="514"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="499"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="564"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="620"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4365303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="687" mass="76885" checksum="4AF89413FBE52E78" modified="2023-11-08" version="1">MADLPRSFWATRGSDSRPSAGTQFQNSDTDLHRPRAVVAYATSTPSEAMIQPGLERISQLLKNVRFPWKSIHVAGTNGKGSICHYASSLLISKRVKCGKFTSPHLVDREKINASSFEILTATAFHIFNEERVDVGVIEVGMGGKLDATNILNNQAVCVISKIAQDHQNFLGTTLGEIAQHKAGILKPKVPYIVNPLNEWHVHDTIDQYAKEIGAGPRLAGDTPELGETLCSDQNWDRFAQALRPFQRDNAILAIVAVKETCRELGLEFAYPDIAEALVKIARKDNPGRLQYLKVQPVFGQPGIPGRDILVDGAHNPDAAQALKDFIFINERRKRYHGQPPKSGWPVTWVLAMTEGKDALQYLEPLLQPGDNVITTSFGPVDGMPWVKPMDPKQLLDMAKTVQPGITGLYTNTKGALRALCAAKHITEGHCPIVVTGSLYLVGDLHREIRGRGDQAWWKDPEHEEDRSYFTTMLEEERAKVNTVLSLKEPDAPDSVTPSRESEEMKRRRLENERRSELLDEIADLDGQMQRLDEEEKRLSRVRHYGLLKAPVRSSRSRSLPEAIPQSSNMSNTQINKSLSNLPEPPEGKYYAAFDSLRQSLQALHIKTPYVDPSNVSVSDSGKREQDAFDLREEEARPSEDGHEKGRREDSRGGRAREDRAGDRTSPEFRKHLSNADKTRPLRRVKSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3P6</accession>
    <name>A0A9W8Y3P6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PDZ GRASP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_008534</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3P6"/>
    <dbReference type="OrthoDB" id="3318at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000026">
        <property type="entry name" value="Golgi reassembly stacking protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007583">
        <property type="entry name" value="GRASP55_65"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024958">
        <property type="entry name" value="GRASP_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893">
        <property type="entry name" value="GOLGI REASSEMBLY STACKING PROTEIN GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893:SF0">
        <property type="entry name" value="GRASP65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04495">
        <property type="entry name" value="GRASP55_65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51865">
        <property type="entry name" value="PDZ_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ GRASP-type" evidence="6">
        <location>
            <begin position="68"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ GRASP-type" evidence="6">
        <location>
            <begin position="157"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="251"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="339"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="361"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="388"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4365912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="399" mass="42170" checksum="296D8748289AD9BF" modified="2023-11-08" version="1">MATASEPQHLSSINTNIPTSPQPDTPQRLSTPKNPHLIGSMFGVLNRFISKLDAAPLDEQQSATNGAYGFQVLRNANQEVPLEPWFDFIIGINGRTIDNPDPSLFATEVRNCAGNSISLGVFSAKGQRIREVYLSIPAEKPTLGVSLQWSPLSIAEDVWHILDVIPNSPADVAGLLPYGDYVIGSPEGLVRGESGLPELVEDFLNRPLRLYVYNHEYNVTRPVTITPARNWGGDGALGCVLGFGALHRIPPSLDEPPQAPGETLFSTDSAPTSFDEKRPISSASTLPPAQVGAPSELFVPANMALPSKSPPPQGSAPPKKKGRVHHGVSPAGGGGGLDDYFKEGEQKSKEEDYAPKKGSGVPPPPKAGGPPRGPPKSGSATPVPSGDAEPEAEAEVEAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3Q1</accession>
    <name>A0A9W8Y3Q1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_007475</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y3Q1"/>
    <dbReference type="OrthoDB" id="5291209at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4366945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="364" mass="39371" checksum="EBAE505F414DB2B4" modified="2023-11-08" version="1">MAVAHADLSKFTDSIFIPFVHEFSSKEVPQVLCTIEGIDIRMPVDTGSTCLLVGAPILPNISATAGTPAHHYFTSSRILYVGRLVELAVGFHGEGGSDATAIVEVLIVDKSWRCPWYNPSKDGFTCPLGPKGETPVERDTGNITYMGVGFGRNQPRDGMPNGTPRVNPFLNLHAIDGLPVSNMSMRAGYIIASEGVHLGLTQENTRGFVFEYLEPGWAHDEDPRDWAMAKMSFGINGEGDNRGPVLIDTGIPQMYIRTEEGVSLPTVTIRNPNKRGHARLVKRVKPGTGISVSFPSLDAPVVSYSFVVGDASYTAPSYVIPANRAGPPYINTGRNFLFGYSIAFDAIGGRFGLRPINRPSSSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y437</accession>
    <name>A0A9W8Y437_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008745</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y437"/>
    <dbReference type="OrthoDB" id="303614at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF11">
        <property type="entry name" value="HISTIDINE KINASE_RESPONSE REGULATOR, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="160" mass="17663" checksum="CA347E48EACC1C59" modified="2023-11-08" version="1">MQKRNLRYEIATNGLEALKAYQECAPKFNAILMDMSMPVMDGMSATRAIRQYEQTNNLPRCSIVALTGLASESAKLEAWNSGIDHFMTKPINFKALEQVLKGEEERRARNAANAAGKLQEVNVPNVEGGKLEANVLKIEEAKADRTVEAQAGNIKDDNIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y484</accession>
    <name>A0A9W8Y484_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007643</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y484"/>
    <dbReference type="OrthoDB" id="3540210at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="471"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="630"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="649"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="680" mass="75880" checksum="E1DE78DFDE1FC4BE" modified="2023-11-08" version="1">MLMSMMAWRSGRRAGRPTLRFLPIIVAAFTISAAFGVASIFSSNVTSETLNQVLLKGTRCGAYSEEKANSIYKQLTLLLPSQSEKASKFLNYGMQCYTNETHADGCNLYIKPKLPLVSTRGLACPYGDNICTLENDNLVMDTGLLDSLEHLGINTAPEHRFQLRFLHTCAPLKTKGYTSDYNDSDYGAVKRYHYGDIANFHQVNNWTFELPVDNAYIPGDNTSSADIPRLEYSLGTQHHYSTPNETLLPGANTWSPIDALKRDDADVYLLFMSADGIHYAGPTDDPWFSAHKNASEIHYQKTKDQFDAWLQDEPLGVMACTQQVQYCNPSLPEGERCEPLRGGADPRKAENVKKIFPTDDHFSVIEWVDNIWVYGVYTISATVGFIGASALRARYGLSYGYSAPLPDDQWQLEAEHWIKGTLASLQDVFVEAANGIPEALEDFRQPPLADETVAQNLCVNQKIVSTNYSSFNVLGLSLILIIGVIVVVLDMGLEPTVAWWQRRTYAKHQARDEGYPSDKIAHPLYAALEWNQTSILQLQRHAQEEAGFGEWTRCATDVPVTEPRQLLAGLDLRRIAHPLLTLKHGTPASEWSGENVIAFKPWGGVRRSDTGLDTLVEEAAAEVEKEGKKVEGEKGDTEVFVGDHNGYGPDDREEARGLGVHLHDRTMEDQQPGQEWRVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4D2</accession>
    <name>A0A9W8Y4D2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inosine/uridine-preferring nucleoside hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_007131</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IUNH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4D2"/>
    <dbReference type="OrthoDB" id="5783963at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008477">
        <property type="term" value="F:purine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006152">
        <property type="term" value="P:purine nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="entry name" value="Ribonucleoside hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023186">
        <property type="entry name" value="IUNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304:SF56">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G11790)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304">
        <property type="entry name" value="INOSINE-URIDINE PREFERRING NUCLEOSIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53590">
        <property type="entry name" value="Nucleoside hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="4">
        <location>
            <begin position="3"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4367547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="214" mass="23607" checksum="5490BD502DEBA296" modified="2023-11-08" version="1">MLLAFAAKPEELEILMLSLTFGNVEVQNCLRNVVTLFHYIEKERAWRKANGRPEGFETLKVRKPIVAVGAEEPLAEHMMVADFFHGIDGLGGIHHSHPHLSPAETWKSLFLPRPDNLSTEEATELEAVKEQHSLFTPSLKSAPEQMLDLLRENEPDTITIVAVGPLTNLAIAAAKDPEAFLRVKEVVVMGGAIEAPGNVSAFFHLIVCPGREMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4G3</accession>
    <name>A0A9W8Y4G3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">PAB1 binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PBP2</name>
        <name evidence="5" type="ORF">N0V83_008364</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4G3"/>
    <dbReference type="OrthoDB" id="1937934at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22439">
        <property type="entry name" value="KH-I_PCBP_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22455">
        <property type="entry name" value="KH-I_Rnc1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22456">
        <property type="entry name" value="KH-I_Rnc1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="127"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="210"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="411"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="332"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4365744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="487" mass="51423" checksum="17F306BF3B415324" modified="2023-11-08" version="1">MSGSPTIAASSAKRPLEEPSSPAAPNEQPDAKRPALDKMVKDEPTADDIDVAQPVKAEGMATEDLKDDEPKPDLDVAVAATQNGAENIQTDTVVPDAPSILETQPIQSTSGANGRPVNHNQQVDESNWLHLRACIGTQEAATIIGKGGENVTQIRRLSGAKCTVSDYSRGAVERILTVSGQVDAVSKAFGLIVRTLNQEDLEKASTSTSKAYPMRLLIPHILIGSIIGKAGVRIREIQDASNAKLNASDTLLPNSGERSLVVLGVADAVHIAVFYVAQTLVEQLLERFGGPAASQYATRSGMAANVVPGGMSVQPYVPQPAGGQYAHTQNFRRQEPQRTPAHGGYGAPMHGQPPQASPYGHPNMPYPAGSPSRAAYGGPVAANPYGGHHAAAPVAHGTAPNHAANVAMPGQPLTQQIFIPNDMVGAIIGKGGAKINEIRQLSGSVIKINEPTDNSNERLVTITGTQECNQMALYMLYSRLESEKHRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4K1</accession>
    <name>A0A9W8Y4K1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Pre-mRNA-splicing factor SYF2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">syf2</name>
        <name evidence="11" type="ORF">N0V83_007237</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">May be part of a spliceosome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the SYF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4K1"/>
    <dbReference type="OrthoDB" id="199717at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013260">
        <property type="entry name" value="mRNA_splic_SYF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13264">
        <property type="entry name" value="GCIP-INTERACTING PROTEIN P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13264:SF5">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SYF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08231">
        <property type="entry name" value="SYF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="55"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4367652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="284" mass="32158" checksum="AFFF649B00F44C65" modified="2023-11-08" version="1">MADTTKSDDEKPSTMTATDSPAEAEASLTEDAKAIPANDPKAAVAARMARFKALQAKKDAGRQATEKEVRDAEDRASRVAQISKLQDANVKASYKLLKNDDPDFERKRNWDYTVEESESWDKRMAKKARNRDGVAFADYRNEANKVYKRQIKQMSKVDQEAYASQKAEKLQSQVSSGLLQLVETSSGEIYTVDSLGRINTPVDEEYSHDHKPSKEAVDKLVEDLEKGERARLKARAARGLRDEQDNGDVTYINQKNKQFNDKLARFYNRYTSEIRDSFERGTAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4R7</accession>
    <name>A0A9W8Y4R7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008516</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4R7"/>
    <dbReference type="OrthoDB" id="3731753at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4365894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="404" mass="45416" checksum="E70480E887EA2117" modified="2023-11-08" version="1">MATHSSEFLDVDEYERKPGLHRSRSGGYADHLEEQVLWDRRDRERQMKRYHDSRYASTATPEQRSTSDRLGVPVFESTRRNRSNSDIARRKEESPAARYNVQEVRPNPNSTYDYSLTSEPALPSQLPVPHKWKPQQHRKKPSINVEIHQDNPPSSTPPSATSTHTPRRSPSASPRSPTAQPQLQYQHATLQTALAQITTTCNAYLEVEAADPRDLTFTKISDRVKGFSFDLQVWAHVVSLDSMAQIDSRKRRVVEAASRGLDRLIERVGELNDACKEAKPRDLKYRALPAVDDDDDDEDMFGNDDDEADGDEGSEQADPTESLGFIIQSSLHSIELQIQTLKRLSRALQEASPDAKDEVVQVGALVEEVAKYFGEQDALDRYGIDGKFAGGKALKEARYAAAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5G0</accession>
    <name>A0A9W8Y5G0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Chromatin structure remodeling complex protein sfh1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SFH1</name>
        <name evidence="7" type="ORF">N0V83_007135</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5G0"/>
    <dbReference type="OrthoDB" id="10258327at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006939">
        <property type="entry name" value="SNF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10019">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04855">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="250"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="493"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="275"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="517"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4367551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="599" mass="66789" checksum="A01100FD2E1B9067" modified="2023-11-08" version="1">MFTTTGKTAPPQAFMSSYAPRLRSHGNSLISPIVPPSNTLPAPRVTKRGTAVLSYAEDAYDDDDFEDSDRPRRATGLRSIQREDPSNVDKTAQIAALGEELSAPIDLQGIWRDWMGRPKRTLTEKQVQTQSALPVTLIPIRIDMDIQSFRPDAPLPTPSNARDFGINESLPAYKQPDVTPAYRLHDMFLWNLHEALTTPDQFAKQFVDELDLPNDRKPYLILSIANQIRQQLEEYAGVALHPLFHSTTSPALQGTTAKPTLLAPSRDGTSTPALRTSTPTIRTSTPALPLTNGTSTPVVNGTTQTDGVQTPAGIPNGISASATALPSEELHNPDDTYRCIVTLNINLMNRLYTDKFEWSLLHPPGFAEMFARTTCADLGLSGEWVAAMTHAIYEAVLRLKKEACENGGLVGDGEIDNDAVEPAVGAGWRYDHEHLCDEWEPKVEILSPEEIEKREGDRERQIRRLRRETARFSSTANMTGTPQNEFFNNIEQQENMGRGERSKKKRRFRSLSPVGRGTPGAGSGYGGQDGGLQEGERQTWRCAHCFVWGTAVWAVRDGPRGPRTLCNNCGYLYERDKKLPPWSENLFYHDRPFHEVRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y626</accession>
    <name>A0A9W8Y626_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006377</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y626"/>
    <dbReference type="OrthoDB" id="3786030at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="398"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="652" mass="75211" checksum="9CB627872FF7F8FA" modified="2023-11-08" version="1">MTKPYNMKVDPLSPLSWDNTDFRRLFEDYWHRRLWDNDYLQSSSDTNEDEDYDGPWKEFYQDNGYINALVPDQSEMLDNEVHPIFGFSNWITHFDYFDYDNLQPALRLASQFLEDPHMLKWWIHLRYGQPGIEGGRVCIKDHPDEHIPEATERIKDQLRKVAPQIKIIFLSHEDIQATGECMGDLRSLRVTFKNACNVAVPDLEYNLWDSTQQITITINEDNYDRVCASCSRQMQLRNHLYLAMTLVHELAHACALIWQPDACFSQESLIQPTDHLPEAGLSWEKFVFGGHIDEHFGPGPPLCAEFDHTYPSTTGVNLPLSAPIPGSYISQWFFKDTWADTTKRLDQLRLPTIRKQTEVYLIDRLVGKEFTSYLYVNGISQSPPYDKFGSAYCMQSGVKDQDTENPSKTARAKKVIQRSTSSSQPDYKSIRNHHKKLNGTSNKAEATSSGNEMQSPESKIPATAADVVAWYARVWYNDLDQAKEQGISIKAYGPHFYSYLRQICCPYSQIDVDAELELPTLEDNEETRKHYRSPVRPCYDCMPYCLSLYHEHKIPAVPFSQRLMTEVTLKRAHFWSESRGPRRVGLKHKRPGMSRSNKFMYSTKRVATLLKKDIASRSQVPNTFSFLSGGDMDSLNVSFRGLMREGVEKTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6D7</accession>
    <name>A0A9W8Y6D7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">E3 ubiquitin protein ligase</fullName>
            <ecNumber evidence="15">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">BRE1</name>
        <name evidence="20" type="ORF">N0V83_007430</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H2B to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the BRE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="15"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6D7"/>
    <dbReference type="OrthoDB" id="654191at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033503">
        <property type="term" value="C:HULC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16499">
        <property type="entry name" value="RING-HC_Bre1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058643">
        <property type="entry name" value="BRE1-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013956">
        <property type="entry name" value="E3_ubiquit_lig_Bre1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23163:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE BRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23163">
        <property type="entry name" value="RING FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08647">
        <property type="entry name" value="BRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26095">
        <property type="entry name" value="CC_Bre1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="20" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10 15" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11 15 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="676"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="709"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="448"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="62"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="187"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="568"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU365038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ4366901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="728" mass="81852" checksum="91C05EE343AE2F11" modified="2023-11-08" version="1">MRLEARTVAPPSLDAVKMEDRKRPIADDSAPPAKRQAVTVNGARSHPDADLPWKDDLEAFQKDAILRQMREFKREKVTLEAQLTELEKKSQHHDDRLRTMDAWFDQLVDEIKVRSAQNLPASSGDPASTPSIPAALFFEDGPTLKKHLSSRKENILTSLAGLFALYPPSSPEVADLQEKLSKLLATEKEHIIRLQRLTTEKEQLSDRLDTATHRYLVAEKKNDRLKSIQIQKLEQQAVATPVVKEEAPSATNGTEAVNGGSPDPCTEKVEAQREYAGAVAEAKKRKEQLEAVELENKKLTEEVSALKIKLTGLSDDDYAKTDLFKTFKNQHEDVIKRINNLEATNIQLREEAQKYQAERTAYRIKIDDETRSSLAESESNVSQAEANLARIRSARDELMAKNSMLETSHKDAHTSTEQSKDLISACESRIAALESECERLRLQIAEGQIAESQTDSGPDESTPEQSRNKISNLENQLKLLSNELPSMEAAWKKAQAIASKKIAELATWEDNIAKAHADKAKADQKFFAAMKAKGELEQQIRILRTQATKSTEVVAQLKETDSLSRSLVDKLEKQTAEMRSQMEELSVQHRQLQQKLSENAIVSEGQISQIVELKKSVEAKDTSYLAAKHAQRDSETERDKLAAQVTVLEKQVDIWKKKSTRNQSSETTIMESMIQCQICKSKFKNTVIKTCGHMFCDQCVQDRLTNRARKCPNCGKAFGTNDTMRVHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6U6</accession>
    <name>A0A9W8Y6U6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006814</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6U6"/>
    <dbReference type="OrthoDB" id="566138at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42678">
        <property type="entry name" value="AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42678:SF11">
        <property type="entry name" value="AMIDASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4368457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="426" mass="46653" checksum="FCF84865E2F70CA2" modified="2023-11-08" version="1">MLAVLDILMVKDNDTTCDFLRGQSFVRLLELEDVRPDSFPSLAIAESLKGMRISVPRVFIGEDDPAAVPNRSVQLSKKFDIPAVTNYDLPPWDAQVETDWPIPHYYNYSGGPNWTFVAAYAWDDFLAMVDDENSIISLAYVNPSLVFPQLPGALPDRYSNQLSNRTGSYDRYVEIAQKRNAEDIFGLPNVGAWLQSLEYRHIADLENWMDDKSLDLVVWPSAGDVGPQDIETNETAAEIGWRNGVARSNGNAAIHQLGIPTVSVSMGAMSDIHMPVDLAFAGKAYDDKALLSCGYAFETAHEKRFAPPQTPELEITSECAGKSAFTRGSEAPLLTAEAMRNGDSSSIIVSGSISSSDSSQVEVFLDVEHVGPVQFNGSEWSVTAAVTAPWFGSDFPEKNVPPQWLAMVVVVASARNGRSNGKLMFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7A7</accession>
    <name>A0A9W8Y7A7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006643</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7A7"/>
    <dbReference type="OrthoDB" id="5391496at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="274" mass="29987" checksum="D89ADA8FFA9FB423" modified="2023-11-08" version="1">MDSIAADPLKQRVGPRCTHSYDSVRTLGKLAMSFAPSVLYPLTLPELLHHILTTQSNTASTTLIVCSSRDAFAQDLASSLQPDHGESSTSGLEQLLIPTLHNLFNTRHVKVAFCASVQALLAYLTAYGGPGTRHIGDSGERERLILVNPLALHAPTPSFSAQGLSRTFAAAAETGLKTDAVLHVVECQGKRRHVPNHREEDPEIVTEDEERHSETEEDDPWEQEVSILNVSARRFGSGAGERAWAGRTIKAKRIVGRWFLFDKVKDHGNPNNPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7Q7</accession>
    <name>A0A9W8Y7Q7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005754</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7Q7"/>
    <dbReference type="OrthoDB" id="3682270at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="53785" checksum="3CFB87F48CB37B3E" modified="2023-11-08" version="1">MASLQQRSHLDRLPNELKLLIIENTRKRVDWKDLSLVSRGWALLVLPLLWDTFKGDLTWEVTRDRRLLADPTSNIAKYVRHLDIQDRSFLGSNLPTCLFAAIPKGQLRSFRCRKGASCIDNLDIMLSSHPGLTELSVAGDQRHYALKLSRLYLGELDFPGAFDNLSQQFDVLALEELTLDGIYGVTGLLTSMAAKFATGKPVLKKLRILTVCDRGTDAFSRSLRSLLESFQGLHQLYVECCDSSRLDVIGIGNHGATLKNLLIVNGGFVRTDASRCITAADVHHIVRTCPNLEQVCLNLYENDRTEVDPALYSLATLPRLEVLRLTNAPEYRKLHHWPNHFDRENIKLRRDVRRLDLQLRADDVMERFDNSVSNIKLLALSPLEDVGEIEVDEDGHTWPNYFYRPASDEGVDVEDSNTDVEEDVKVKPKRNRQRELAAALSAARVVSSDNELSQTSKRRNMSKRNTIQKMRGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7Q9</accession>
    <name>A0A9W8Y7Q9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_005626</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7Q9"/>
    <dbReference type="OrthoDB" id="2270193at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057683">
        <property type="entry name" value="DUF7923"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37543">
        <property type="entry name" value="CCCH ZINC FINGER DNA BINDING PROTEIN (AFU_ORTHOLOGUE AFUA_5G12760)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37543:SF1">
        <property type="entry name" value="CCCH ZINC FINGER DNA BINDING PROTEIN (AFU_ORTHOLOGUE AFUA_5G12760)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25540">
        <property type="entry name" value="DUF7923"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25542">
        <property type="entry name" value="zf-CCCH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="346"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="346"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4369862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="434" mass="48696" checksum="D4EBBE12145982F4" modified="2023-11-08" version="1">MADLHLFLQEMQRFRASDEARERLVNEVLAKYSTLSEEHFILKNDYASERDIRRNYQSTVEEKQSLIREYERQLESSSFVLALIDGDGAIFEDALLQAAAGDGGSEAASRLYQAIRNHVAAIYSNSGNWPVMVQIYLSLDKLAMKLAQVGLLRAPQELRAFAQRFSVNQPLFSIIDVGQGKERADHKIKGNFSLKVLLSGALTCVTEMLRTFSDNPTCRHIIFGGCHDAGYLLNLEHFKHNPSKAARITLLETTPAYRGFAELSNFTRARFDDVFKTGPLPDYAPPPLSKSGAPANGSISIAPSSNTKTNTKKKYAYYNKAEQRLDEALPPRDPAAAASLEARMKKTGKKMCNHWHLGGHCENGKFCNFQHEPKLSPAELNALRYKTHLGHQCALERDQGYCPYPDNCHLRGTHGMDRVKHVRYDKDGNADYAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7T2</accession>
    <name>A0A9W8Y7T2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ELP4</name>
        <name evidence="10" type="ORF">N0V83_006555</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ELP4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7T2"/>
    <dbReference type="OrthoDB" id="289162at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19494">
        <property type="entry name" value="Elp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008728">
        <property type="entry name" value="Elongator_complex_protein_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12896:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12896">
        <property type="entry name" value="PAX6 NEIGHBOR PROTEIN PAXNEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05625">
        <property type="entry name" value="PAXNEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="12"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="372"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="380"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4368199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="398" mass="42878" checksum="9F699A2C3C68FBD4" modified="2023-11-08" version="1">MAFRKRNIALSRTPVGEDASNVPSTLPTAPPAPTPLGVRPSPIDGRPTTSTGIPSLDGILAGHAGLALGNSILIEESGTTDYAGALLRFYAAEGVVQGHKVHVVGMGEVWGRQLPGISEDKGDKRKEGKERAEKMKIAWRYEGLGQFESARGSPSPNRGAAQGENATEEPSIFCHTFDLARRLTLPVGTAINYIPIPRVASDPSSIFPPILQNIQRQLDSTPPHTTHRLVLPSLLSPALYPPASAHPSSILQFLHALRALLRRYPTRLTAISTLSLSLYPRSSGLVRWMEILSDGVLELSPFPYSHMQALAQSAGTTKDEERPQGMLAVHKLPVFHEKGGGGGVEGLGEDMAFTLSRRKFVIAKFSLPPLEGDTEAQEQAVREAAGDSGMPRKEDLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7Y0</accession>
    <name>A0A9W8Y7Y0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhamnogalacturonan endolyase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_005968</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7Y0"/>
    <dbReference type="OrthoDB" id="2130367at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10316">
        <property type="entry name" value="RGL4_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10320">
        <property type="entry name" value="RGL4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051850">
        <property type="entry name" value="Polysacch_Lyase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029413">
        <property type="entry name" value="RG-lyase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029411">
        <property type="entry name" value="RG-lyase_III"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32018:SF9">
        <property type="entry name" value="RHAMNOGALACTURONATE LYASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32018">
        <property type="entry name" value="RHAMNOGALACTURONATE LYASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14683">
        <property type="entry name" value="CBM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14686">
        <property type="entry name" value="fn3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040769968" description="Rhamnogalacturonan endolyase" evidence="2">
        <location>
            <begin position="22"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="domain" description="Rhamnogalacturonan lyase" evidence="4">
        <location>
            <begin position="352"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Rhamnogalacturonan lyase" evidence="3">
        <location>
            <begin position="441"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4368886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="659" mass="73786" checksum="E83D8C752C447256" modified="2023-11-08" version="1">MRIYNIVSLLLPSASWAFLNAVENNSSLTIFNERLVASVSKSKGYVNKLTLDGQNLLGTEDGSTGVGPYLDCYCIPSGFWTPGRGSNVTYNLYQGTDSTGTKYGGISMGETYAPTGQRLEQYWFLREGETGLHTFSRVAYYNKTTPFLRNLQELRTMFRPNHDPPLFTHFVTNEKFAAPRPDTEGEIVVQDATWYLPNKDDPYVTGVGDYFTKYTFQDTWRDHKAHGMWADGSGSTNGTTYGAWLVHNTVETYFGGPLHSDLVVDGIVYNYISSNHHGDQTPNITDGFDRTFGPQYYHFNTGGSLDELRQDAEQYGHKPEWNAKFYDSIAKHVPNLVTSSARGTFKAKIDLPKGAKNAIAVLAQNKVDYQDNVYDTKAYQYWGNIDNNGEVTIPRVKADKYRLTIHADGVFGDYTEDDIVITPSKNTSIHSKWTAESSGTELWRIGVPDKSSGEYRHGYAPSPTHPLLTEEYRMYWAHYDFVDEFPEGVMFRVGKDDAATALNYVHWSVFGGYANYERPTPVYDNINNWTILFDTTAHQLIGKKTATFTVQLAGAKTAAGNTDIYNASEPYSNLPYTVNINGADLAPWVIPYHHSSSCAVRSAVICYNIAHKFSFDAGMLKAGGNTIVLSLPYNATDYESAVLPQSVYVQYDALRLEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7Y2</accession>
    <name>A0A9W8Y7Y2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA polymerase delta subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7Y2"/>
    <dbReference type="OrthoDB" id="337486at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000731">
        <property type="term" value="P:DNA synthesis involved in DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007218">
        <property type="entry name" value="DNA_pol_delta_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14303">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14303:SF0">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04081">
        <property type="entry name" value="DNA_pol_delta_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4368274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="22008" checksum="07AE566CE344A4DC" modified="2023-11-08" version="1">MPPKRRSTGPATKAQQSTLAFHGASNKVTKAGARAQGAKKNLPDQSSTKDAKPEVIEIEDSEPTTAETAIIDQTEQEIKAQQVDSTPEEDQARRVTDAAIKKYWAAKEKQRMAPRVHQQDLTVHEKILREFDMSAHYGPCTGIARIKRWKRAHRFDLDPPIEVLAVLLKEQKVKDKLAVQRSHVDELLNSKAELEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8G4</accession>
    <name>A0A9W8Y8G4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial distribution and morphology protein 31, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MDM31</name>
        <name evidence="12" type="ORF">N0V83_005088</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the organization of the mitochondrial membranes and the global structure of the mitochondria. Also required for mitochondrial distribution and mobility as well as for the maintenance of mitochondrial DNA nucleoids structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MDM31/MDM32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8G4"/>
    <dbReference type="OrthoDB" id="17678at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012571">
        <property type="entry name" value="Mdm31/Mdm32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31068">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31068:SF0">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08118">
        <property type="entry name" value="MDM31_MDM32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="229"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="105"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="581"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="108"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="584"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4370567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="726" mass="81107" checksum="40AABE804B99B025" modified="2023-11-08" version="1">MNKNVGTIGASLWRSTVDQPLKCGALLRRFEWGASSQSIWRTEIQRRSIASFPQTCSCSTSSLRPGLPSSQFRPLSTSLLQSSAPRPGHIQQSVTVAVLSAWSRHASNKPSRASKRSSGGSQRYKSSTTEGSTKNKRGPTGQAPQATLSPQPESPPKASSAPPKPKAKPEGSTASSKPLIDRLPHSLPHLYRPSKAELLEAASGFWSRIKIHFKWLTIRSARPFNADEIYALFSWILAAHVLWIVLGTTTFFMLAIFMINTAFSQETLGKWIGNYLTKSSGIKVVFETAVVPKWGDGVISFRKVFVSRRPGQGRGKVTKGSQTEMAAAAAAKAQHEKDVKDGVPAAAETVEDTNYTQFDISIDTVNVTLSFSKWFNGKGLLKDVEIKGIRGVVDRTSVETIEGVDPRSYKHEHSPGDFELDSFKMEDLLVTIYQPHGFRPFSVSIFSCDLPQLRKQWLFYDFLSANMMSGSFDNSLFTIHPRQTHNYTGAQLSSGRDSEDGSSWKKHSRIRIDGLNIDHLNRGYDGPFSWIHEGNVDIVADVMLPNDDDESLAKVMSDMYDRVEATVSQNGRKHHFSDHAAQGFDEHHPNDEKHDHTHDSPEDKRFMIMDMRVHLNDVRAAVPIFTRDISYVNNALVRPIVAYINSKRTFIPVNCRVVKRVSEFDGSWTLYDSGLMEEVSREMYDAFARDVLDDRTVRKRRIKKVGIWTLQLAAQALFLGLAGNIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8T9</accession>
    <name>A0A9W8Y8T9_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">MFS sugar transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MFS1</name>
        <name evidence="12" type="ORF">N0V83_005241</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. TCR/Tet family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8T9"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17502">
        <property type="entry name" value="MFS_Azr1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000489">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000196">
        <property type="entry name" value="MFS toxin efflux pump (AflT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000012">
        <property type="entry name" value="MFS toxin efflux pump (AflT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF199">
        <property type="entry name" value="MFS EFFLUX TRANSPORTER INPD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01036">
        <property type="entry name" value="TCRTETB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="88"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="391"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="448"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="481"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="556"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="91"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4370720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="589" mass="62947" checksum="F02156BC3C35AA94" modified="2023-11-08" version="1">MSETNKELRPVSMADSEKTAIQSHEPSMLGEKGVVEGVLPSTAAAVEASDPAVIDEKAAAKETSVRNSTASVEEGEEDDFEYPKKGPLAAITAALCLSVFCMALDNTIIATAIPRITDQFKALNDVGWYGSSYLLTTCATQLIYGKFYTFYSIKWVYLTALFIFEIGSLICGVAPNSTALIIGRAVAGIGAAGIFSGAILIIANTVPLRQRPTYMGLVGGMYGIASVAGPLMGGAFTDHLTWRWCFYINLPFGALTAAFIIPFFKINRRGKKTEATWKQQIQKFDLPGTFFFLPCVICLLLALQWGGSKYAWGSGRIIALLVITGLLAIAFVVVQWWKQEDATVPPRVFMNRNVWGSAWFGAMLGAAFFVMVYYIPIWFQAIKGVSATKSGIMNLPAILGLVIVSMAVGGAVTALGYYTPFMLTSSVLMAIGAGLLSTFKTDTNSPRWIGYQFIFGAGVGAGMQQTLIAVQTALPAEDVPVGTAMMMFAQTLGGALFISVGQNVFTNQLIKHLGAVVPDLDPSIVLVTGATELKNAIPAQYLEGVITAYNLALTNVFYVSVATATASIAGAAFVQWKSMKGKQITMAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y8Z8</accession>
    <name>A0A9W8Y8Z8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CAP-Gly domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_005499</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TBCB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y8Z8"/>
    <dbReference type="OrthoDB" id="5295208at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043014">
        <property type="term" value="F:alpha-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01789">
        <property type="entry name" value="Ubl_TBCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045172">
        <property type="entry name" value="TBCB_Ubl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF85">
        <property type="entry name" value="TUBULIN-FOLDING COFACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14560">
        <property type="entry name" value="Ubiquitin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CAP-Gly" evidence="5">
        <location>
            <begin position="188"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4369736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="245" mass="26849" checksum="4377EE70A2BC4ABC" modified="2023-11-08" version="1">MSLQSAADVPLIISSPNSSSERRISPSWSIAHLKTRLEPITGVPASSQQLSLRLGSQDAIPLTAADEEQTRLVAFPLQPYAEISVVDTRPPGARTDFTDLSSVEKYEMPTTEYESLTNSVLAWKKSQKLGRFDPNAGTIEEQKIRASEREVEERGLTLSSRVRLLPETDARRGTVSYVGLVPEIPGLGSWIGVTLDEPTGKNDGSVKGKRYFECGNNCGVFVRPERCEAGDFPPLDLGDEDLEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y912</accession>
    <name>A0A9W8Y912_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_004343</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y912"/>
    <dbReference type="OrthoDB" id="5351220at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4371127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="622" mass="69620" checksum="6C63670220BFDC32" modified="2023-11-08" version="1">MPADVFPPVIIDTRVTYRIGSPLLPAQPVHTIWRLSIAEWWKDGFRQEVDKILREDNVYVKQLGVVKRVQRGNDAQTIPKTILIVSDISVPDQRENWKRATSNLRALLRSRGRDELFIEIIDAKAVFGITSAAIGDDDSATVTAWKNMSRQFLEEVSDRDWLTIDVVHRKVGEHVPKKIPTICITASDALDAVWWDETIPRLRDVASPNFEIDLFYATHVLNTMDEHERIEDEDEGSEIFATIESYRNDVQMGASIGVDNSNTSGTVSACVELCTPKGEKIMCALTNHHVAATSDELANECPVGQYLDPNHRLAREQVVRIMSPSDKDHERLKSWNSMQVRDLELVEQMRKERGKDCNVIDKRLLAARNDATKLGQDGGVSPDRYLGAVWASSGHRTAPNTTYTTEQARRWSRIRNTNETCQHTNIRNQEASLAELINGSTLEWGLNWALIKVQAPRSLSSDVPASGSRVHIPEGALCTRYGDIQPERCYSVAKLGRTTGWTTGEVNQIESLVHLRYDNTSVVPIDLRKRFGKIVLAYAIVSDVEQKDFIAGGDSGSVVLLNERNPEATMLGLGFASNKATFVGYMLPMSHIIEDIERVTSSKVTMPERVAMASGDFILDDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y932</accession>
    <name>A0A9W8Y932_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribosome biogenesis protein ytm1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">YTM1_1</name>
        <name evidence="4" type="ORF">N0V83_005135</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y932"/>
    <dbReference type="OrthoDB" id="534815at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990423">
        <property type="term" value="C:RZZ complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046362">
        <property type="entry name" value="Zw10/DSL1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055148">
        <property type="entry name" value="ZW10_C_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12205">
        <property type="entry name" value="CENTROMERE/KINETOCHORE PROTEIN ZW10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12205:SF0">
        <property type="entry name" value="CENTROMERE_KINETOCHORE PROTEIN ZW10 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22766">
        <property type="entry name" value="ZW10_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZW10 C-terminal helical" evidence="3">
        <location>
            <begin position="705"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="441"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="43"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="452"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="479"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="489"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="514"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4370614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="858" mass="94764" checksum="B1629C1A5B5DA7A7" modified="2023-11-08" version="1">MPSQVSDEKLGDAILQSAEHGAFPQDEDVASATVPSSALPKLLEIVGKARENTKNEVRNLSREAAPDVDGWIKQARKLQDDIKRSQDTAKEIVQQAEGGKENTLRVQDTASKVSLLHSEIAYNESLAEVVEQLRDISTLLDSAQNAAVHGHVMHALQTLDDADGAFERLGPFETTRAVGVLKNKQGQLKKAITETLTESWNGLVNVDNTNHKISLRQSIEREATVDINGVVEALATLGLLDSFIARLSRDLDKAIISPRLVQSPDHPLSAFDIQGDDIQRAGHVNDGSVKAALEDIHKIAEYLSTRLPSNVAVPLSSKLVPIVAARLISNFLQPAVPVSTEGVLEFQDTLSYVLGLVEFLDELGWSGQHRLSDWVDKSAEIWLSKQKEAAIAKVQTLFPKRVKEKIAVERVETQVISKGDALHTGGNDEQDDDWGAEWGDEEEAAKQKEQNDSQEMEEEDMSAWGEVDEANTDQSTEAAKIEEQKPADDENADEWGADWGDEDDTKTAAPLESTAESVEQQKVNGKSASQRPPKEQEVTLRETYTVTAIPDSIMEIILQVVADVETLNSSDLAKTAIAPASGGLFAIPSLLLAMYRATAAVHYSKDVAGNMLIYNDCQRLSDRLRIFLQERIDKDKSSALPQLLRASNRLKATLEADIKTIDGFGKRAYGREMESQRQIIRDHLEDAQGFQGCTNVPFATVCDDAIATTIDRIGDVKRQWQNILSHSALLQSLGSLLSTALTKFVNDVEDMSDIAEEESKKLHSYCVSLTTLSQHFQTEDGSGEMRDTVSIYTPNWFKLQYLGEILDSSLADIKYFWTDGELKLEMEAEEVVGLIEALFAPSDHRRRAIAEIRRTSMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y995</accession>
    <name>A0A9W8Y995_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_004506</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y995"/>
    <dbReference type="OrthoDB" id="4095124at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055449">
        <property type="entry name" value="Iec3-like_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032742">
        <property type="entry name" value="Iec3_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24244">
        <property type="entry name" value="Iec3-like_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14612">
        <property type="entry name" value="Ino80_Iec3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INO80 complex subunit 3 N-terminal" evidence="2">
        <location>
            <begin position="32"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="INO80 complex subunit 3-like middle region" evidence="3">
        <location>
            <begin position="158"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4371289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="368" mass="40291" checksum="60DA73D5570B1EA1" modified="2023-11-08" version="1">MASEASPQVDTQLHEAPQAEAPADATQKPVKRSWRRKYRKMRIQFDNAMTESNNLISQEWKAMGTARRLQETNDQLLDALLDFNDQTRVNARLRYDLRQLSAADTAVPSLEAGPDPDFIQQQLRELRDDVAAGRITAEEYTQRADQLHSSQAIQQTLKSLASLEAKIPHTTRADLGEQPIEGLDLSEHAPGYMSPSHEEEYLLAMDQVLAEPGFDPALQMGRPMRLPSAQPPLGEKDLTVRNPDSVYNWLRKHQPQVFLQDKDAAHHENLSEKSAAKSGNAGGGGGRKGVKRESAVGSGTPGPRDHDDEDSTAADTGKGRRKTGGDDDTAYRPKGGSSRPAKRKREGDDTPAGKGGKKKSRASTSAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9B2</accession>
    <name>A0A9W8Y9B2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_004509</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9B2"/>
    <dbReference type="OrthoDB" id="10662325at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="353" mass="39599" checksum="7AB3C3AB67B68C55" modified="2023-11-08" version="1">MPTPTNIDSSLSPPHLDIEDTTDVVDTSPLPTLSAAAPEAQSELNHVDSIKYFRSSTCDGSLIPGTDNGVTRRQAHSFLYHYACLKRHLTANLAILQQYGEDGLSVTTTSPTDHDADTLSPLETAQNSFAIHHIGISETLLSSMPHNPALPSNQDFRISRNFFAHDFGTPKEEWDAERNLETQVKGKTALYENLLKVVEANRTLLEEQLADKSMWRELVLPEQTTAVVSWGAWGTTRDQEGMVYEPQFELPPYEEEALQGPGCLISSFSFLFSRSRFVRNVSERVNRCIQTARNEFYWDALDSEVGTGEEWNAERETESEEEGVAEAEADAEVVEDEEEDEPVDEEDGPVYHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA15</accession>
    <name>A0A9W8YA15_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_005412</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA15"/>
    <dbReference type="OrthoDB" id="8061355at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001345">
        <property type="entry name" value="Related to ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF36">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="220"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4369650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="540" mass="59421" checksum="154DBACF38E4B2BA" modified="2023-11-08" version="1">MVRSMQYSNGIRPLPLWLSHLSFDAIFVLIISIVATALLSISTPVWTGLGYIFIIFVLYGIVAALLSYVISMFAKGAVTAWFLLALGQIIFYFAYFGGILGVQSATAYPDLEYMMNALFFGLGIVSPAVCLERTLFIGLQMMALSCNQRAPGSIYLYGGPILYLILQGVFLFALLLWWDVGFVIPTSRSRKSTTDPEGTEMYSTDLISERKRLVSNQTDLRVESVSKFFGKNLAVDNVTFAVQQSEIFALLGPNGAGKSTIISMIRGDIKPSTSNSTIDIAGHSILKHAVAARANLGVCPQFDSADVLTVKETLRFFAKIRGVKNIDHNVSTVIAACGIGEWTDQLAQKLSGGTKRKLSLAIALVGNPRVLVLDEPSSALDANAKRTLWRCLQTISKGRAVVLTTHSMEEADALADRIGIVSSRMLALGEREDLKKRAGENFYIHIVSKSAPRTTRQELQVIRDWIHGTFQNVKISRETQGGQIRFEVPAAGWDMVGLIREFEGAKERLGIEFYSVGKATLDEVFENIVRKYGDDDVIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAA5</accession>
    <name>A0A9W8YAA5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PEBP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAA5"/>
    <dbReference type="OrthoDB" id="2506647at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030414">
        <property type="term" value="F:peptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030162">
        <property type="term" value="P:regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046578">
        <property type="term" value="P:regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF148">
        <property type="entry name" value="CARBOXYPEPTIDASE Y INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4372107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="21753" checksum="D9BE214BEF886F63" modified="2023-11-08" version="1">MVIDDFLPSLTLSVTWSKEQAKLGNTIKPKYLQDQPTISLHDETDHDSFSTKPDLTYIVTLTDPDAPSRDNPEWSEMCHWIASNVSLSQKTFPILPLPEFELTENDVSSTGSEPDDVVEYKPPGPPPKTGKHRYVFLVFAPKNGTTEALKLSKPKDRKHWGTGEERGGVRDWAEENELVPVAANFIYSKNKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAP6</accession>
    <name>A0A9W8YAP6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cellobiose dehydrogenase cytochrome domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004106</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAP6"/>
    <dbReference type="OrthoDB" id="507128at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09630">
        <property type="entry name" value="CDH_like_cytochrome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1210">
        <property type="entry name" value="Cellobiose dehydrogenase, cytochrome domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054539">
        <property type="entry name" value="Beta-prop_PDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015920">
        <property type="entry name" value="Cellobiose_DH-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH_b-prop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47797:SF5">
        <property type="entry name" value="CELLOBIOSE DEHYDROGENASE CYTOCHROME DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47797">
        <property type="entry name" value="DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G05805)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16010">
        <property type="entry name" value="CDH-cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22807">
        <property type="entry name" value="TrAA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49344">
        <property type="entry name" value="CBD9-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="Soluble quinoprotein glucose dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cellobiose dehydrogenase-like cytochrome" evidence="1">
        <location>
            <begin position="30"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroloquinoline quinone-dependent pyranose dehydrogenase beta-propeller" evidence="2">
        <location>
            <begin position="260"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="653" mass="69432" checksum="E121BED703926FA6" modified="2023-11-08" version="1">MRYAQVLGSAAALAGYALAQEGEVKSTVVYDAETKISYSSYSDAESGMTFGVALPKNVTDPYDAIIRITAPLANKWAGFAWGGDMVWDPLSVAWANGNTSTVSARFAFSISMPNPYDQAEHTYLKGTRTNSTHWQVNALCKGCTGWQSNEDVRYALNGTGTTQFAWAYGVSAVQDPARNDSAFNVHQAYGKWTHDLNAARNDNFESLVKANMLTAPVASSAPTTVVTSVKPSATASKPAGVAAAIPATCSGAGAPTFPGSLASGWKATKIAGGLTSPRSIVFDTAGNMLVVQAGKGISYHVMGTDGCITSTKMLVSLNSLNHGITFSIDGKTLYASSMTQAYSWPYDATAATVGTRTTVITGMVNGGSHLTRTLAIAPHAPNLLVVSHGSNANIDTGASDPKTGRAIIKVFDLTAIPSGGYTYASAGYTAGYGQRNEVGMCFDKNNMLWGVENSGDDFKRNGKDVHQNNPGEKIHYMGDVSKPNNNWYGYPTCFTVWQPSDFTDKTFKVGDWFVQAPSGTNTDDMCVQKATAPKLTLFPHSAPIDCKFDADSTTMYITYHGSWDRSPVTGFKLVAVDFKLGADGQYTPVEPLTSTTAAKDIFYNPNVASCQGNGPSFSSGCFRPAGLAWDKSGRLFMTSDTSTNGELWVLGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YAV0</accession>
    <name>A0A9W8YAV0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleolar protein 12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">NOP12</name>
        <name evidence="10" type="ORF">N0V83_003860</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in pre-25S rRNA processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRM RBM34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YAV0"/>
    <dbReference type="OrthoDB" id="442677at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF25">
        <property type="entry name" value="RNA-BINDING PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="337"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="438"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="66"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="150"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="197"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="438"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="499"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="518"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4372087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="541" mass="60186" checksum="D7EFE0CC9571544B" modified="2023-11-08" version="1">MAKSKDKRSAEAESQQKRDKSLIQIDAKSFDPTLSSLFASSAGPVQAPPKSRYQERIQRQQSEGSSDADDEVLSELESEPSDAEAVSEEEDDDEEISSANSDASSEDESEGEQSENEAVLAKLRESALNGAQEKPNKKRKRGDKEEIEETYMRKLAREEEKEEQQRQKKRKSERAAEEDEDDEASEAEEQEEAEITIPKHESLAETTDKDASELEKASRTIFLGNVSTECINSKSAEKALKKHLESFISDLADNKPPHKIESIRFRSTAFASALPKRAAFAKKEIMDSTTKSTNAYAVYTTTVAAREAVKRLNGSVLLKRHLHVDSVAHPRKVDHRRCVFVGNLPFVDDESQTPNEDGSEKKKKKTPSDVEEGLWVHFSKVGKIESVRVVRDAQTRVGKGFAYVQFIDENGVEAALQLNEKKYPPMLPRILRVTRCKAEKKNKDKPRGPPPSSSKSPRANGKAGYAPKMTEEQKSMQGRARSMLGKVAKAQTKESFVFEGHRASERQGKSGLKFKGSGGKKKGPNGRKARSSAWKKTGAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB96</accession>
    <name>A0A9W8YB96_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="6">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_003346</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cell membrane</location>
            <topology evidence="1 6">Lipid-anchor</topology>
            <topology evidence="1 6">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB96"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000038">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF8">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041012121" description="1,3-beta-glucanosyltransferase" evidence="6">
        <location>
            <begin position="21"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="301"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="371"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="376"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4373055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="435" mass="46139" checksum="C8F3EFC9665FA4FA" modified="2023-11-08" version="1">MVHTRSLLLSALAGLSAVNAISTIEVKGSKFFTSDGDQFYVKGIAYQLTPEDPLIDATQCGLDAALMKTIGTNSIRVYHVDPDGDHTDCMKAFEDAGIYLWLDLDTFNSQIEQISPHWNQSQLTAFSKVMDEFQQFDNLAGFFVGNEVLTTSNGSVAAPYVKAAARDIKAYRTSKKYREIPIGYSAADIAQLRPMLQNYLACGGNSSETIDFFSLNAYSWCGDSTYEQSGYNVLEQNSTELHIPIFMSETGCNTVRPRTFSDQSAIYGEMADVWSGSIIYEWIEEANDYGLISYGAKVDPASPSAPPDGYPRSGTPTPISPDFPNLSSVWKTLSPSGVKASAYNPTNSPVACPAFTSGAWEVNPTAALPTLGQEYTSGSSQATGASGSGSSSQTGSGSGSASATASPGAAAESLSKELKGVGAVVGGIVAVLAWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBN2</accession>
    <name>A0A9W8YBN2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003318</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBN2"/>
    <dbReference type="OrthoDB" id="3642826at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="708"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="712"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="724"/>
            <end position="742"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="765" mass="76857" checksum="E536BF9C5D104012" modified="2023-11-08" version="1">MTTAAPNFTNADSYTLITNTATFDAASALESDYACTSSYSYVSEWDFTITMCMEYTEKPTAAATSIAYRTAAAPFPPDGLIPVTDAYLYDLYSDGQPSATATISIAPNVTHIETSATPFVYFTAYEIESGNTTKTVQLRSTYAYPYWLKGVEQEATATGSLPEGFLDQIPQSACDAGQLQATVTVLIVVDLYYQNWPNANPFIVHVESSVLGFDDPTVGINNDGTSRGVPLTIADWDLPGATTPTSVSLKPAQQPDPAPTTHAGNHNAATQEPKMPNEPQPTQVTVGTIGTAPVVVGPSSEVVVGSQTLRPGGPAITIGGITTVSLAPSATAIVVGGTTSQLPQVFVPPAQSAARPPPVLTIGSSTLTPNAATQFFVAPGQTLTPGGVATVDGTVVSLAPSASFVVIGGSTQLLPTAAPVATSPPEIVVGGTTITAQPTLGRSGNSINQDNSSQGPTFVVWGQTLAPGGPAITVSGTTLSLAPSGSFVVINGITSTVADPAALATSSPTLTIGGGVFSPVAGSGTTFVISGQTLAPGGSAITVSGTTVSLAPSASFVVINGVTSTIANPSAAQITPPPLTVGNDVFKPLPGTGTSYLIGSSILTPGGIVVVAGTTISLAPGATALVINGQTSLIAPQAQNVITNAPLLTVGSQTYTAVAGTTFIIDGQTLTPGGTITVNGTTISLAPGATELIYGSSGRSTTTALFPATTTRSQSVTSISNPRAGASGSNGQAAATSHQGAAASKSETQSWILSSLLTIMGLLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC04</accession>
    <name>A0A9W8YC04_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003581</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC04"/>
    <dbReference type="OrthoDB" id="3690573at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="321"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="561" mass="61828" checksum="2A43E6A5AA7991E0" modified="2023-11-08" version="1">MVSTRRSTANDASTNGMSKPNTSNRADDTIHVLVPSKTPSTPTALKLASTAPKLARPGSISEHDLSLTSTPVHDSSDKVTMSTPTQENKLPSKNNNESSSILQTLALRPASVSGGALNPIVVTDSPPRFRRPQAPKKKQKRQTEPHRFMDNGYRNLYSHRPLRPALAATHANRSTFTGHKSHDIYRMMSAKMAAVPRRDEDRTATYRAFRDFDVRHPILAQHLVRYPVVQYQQPGPYIQYSNQGAPAAAVHRPVPAQNEATLRKKAVQYVREYSRPSPRKRRLSDADPDETSTSEFEETQTSISSHSSPVKSTTRRPKLVPPSLLSLSSSPLTSTASGSKLTISQDRSKFTTTTADSDPNFDINQLTEHTSLITSLFQAYPHSTDQRGLREDIAMLVSVQNQRMRAWMKSEKQLARKRKQSEADSGAITTTATSGVFSSHLHVDGTVDEPSVASDEDLAVVMEEALQRHRRLEHEQEKRKERDEEMRQLFSSTAEMWQGGSSGETLADVYANESDASSVVGSSDSMITCEDGGAWSPAARKGRGFGFGFSFEGVGYDGGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCD2</accession>
    <name>A0A9W8YCD2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dirigent protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4371956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCD2"/>
    <dbReference type="OrthoDB" id="3426753at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040757637" description="Dirigent protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4371956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="18582" checksum="54A839DAEC44BEB7" modified="2023-11-08" version="1">MLRSATIALAALSAIASAANITTLKPSSLTPFLHMDLKVEDSTFVTTTNGILTGQANNQGGTFSGNITGTVLKLGTEYETFPTAGMQAQSTYDNVFSLNTTDGTAMLLTVAANIHYTPNADGTIQLRGFGRMTFGASTPQYEYLTYGDYVTEFEAIYPSGKVSVDVFELGSSGHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCN1</accession>
    <name>A0A9W8YCN1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">GPI transamidase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GPI17</name>
        <name evidence="12" type="ORF">N0V83_002698</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCN1"/>
    <dbReference type="OrthoDB" id="28748at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019540">
        <property type="entry name" value="PtdIno-glycan_biosynth_class_S"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21072">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21072:SF13">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10510">
        <property type="entry name" value="PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4373959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="567" mass="61980" checksum="C1FB6E80A8AA2C29" modified="2023-11-08" version="1">MASVNASDASLAPSGEQPTSQPSSMPLDAVRSARQPPPETKEGLWLRRLAILSFWVVVVFLGLPVWLKTTAIYRAELPLQDMAGWAEGKACKPGFPLRIAVEAPILPSDARHLLRTTQHALDDLNEFSAHHLRLILADSPLQSNSSQQIVSDGTSIRSNDEDVVLAVRLIPVETGVTPRSHLQPYSPALDIYYSPSQIPPQSSTASPLATFLAHELHNLFSEEQAQLTHLFLTTPGAKSANTKTLSPETLAELDRQSTKALRYAPTYHLTFSLFSPTHAPSAWDIEAALHAYLAPLLESFSAISNFTVDTQVQLYATFSPSNRQPDYDEELKAWTLRKEDLSGFINAAEWPLSPSIGEGPTVNFVLYVPDQAQSPLLVKENAGNSWLIPQWGGVHIMNLQDEAGTPSTLSVEALAPAMQTFSNQLVSLFGLPQTPASLPLRISTLERVRATSLIYSASSTLGALAQVYRKLPSIPVPDKVAQSTHRTIAHLQQACDLLRDGRFHGALEYARAAQVEAEKAFFEKSMVGQVYFPDEHKVAVYLPLLGPVAVPLIMAALKELKSALKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCN6</accession>
    <name>A0A9W8YCN6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Short chain dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCN6"/>
    <dbReference type="OrthoDB" id="5327538at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051935">
        <property type="entry name" value="HSDL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42808">
        <property type="entry name" value="HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42808:SF4">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="32757" checksum="F34FFCF31E7A5CFB" modified="2023-11-08" version="1">MPTPQKQVALVVGASRGIGRQIAIDLAKEGYAALPPDPNSRDSTISTVAREIQLAGGTASPIAVDVRSPTSIQALVSQTISIYGRLDVLVYNSGAIWWSSVANTPLKRFQLMQQVNVEGLYATLQASLPHFEKKGAGGGEGEWKGRIIVVSPPIYSRFFRGKAAYAVGKVGMSVLTKGLAMDFQREGKAGMAVTSIWPATAIQSAATQNLAREDARELRHASIFSDAVVAMIKAPVEEVNGELLLDEDFLRTKGVSDFEKYSVVPMATPRRIMPVEFPDLRVREQDDEGRRMDSAKLRAAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCT6</accession>
    <name>A0A9W8YCT6_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">WD repeat-containing protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">WDR6</name>
        <name evidence="8" type="ORF">N0V83_002763</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat WDR6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCT6"/>
    <dbReference type="OrthoDB" id="5594999at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051973">
        <property type="entry name" value="tRNA_Anticodon_Mtase-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14344">
        <property type="entry name" value="WD REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14344:SF3">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="35"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4374024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="684" mass="74485" checksum="E822C3DA859164B3" modified="2023-11-08" version="1">MPIIVAAGTAFGEIIYWSWSHNPGVGSVSRIHRVFLGHEGSIFGVQISKELPSGCCQELRRVVASCSDDRTIRIWDVSDVDTIAATSEGEEHHEHVLRSRHTGFSNESFDADSFASSNCLATGWGHMSRVWTVRFLDPPSRNGSLFLLSGGEDATSRTWELVPNDDGKAQFPYKLSHLAYVSQHSGKNLWSTTVAPGSAGLPQVVCGAADSKITTHSIAHLWQNTMAQAKDGTTGYTLHDIYSLTQPPIEASHTAASQHRNQSSKKADFLRNYCFIDECTVLFTTNSGRVLSGSLATDASSQSGLLTHTTLVAQLDDLFGYSVCTSGVQPGLAFIAGAKGSIYTYSQNTGALSKLHSINGKVGEMHTASFKIDAGQEIVALLITIVGQEDAQLWYVDTADDGGIFYVTAVPISDGLTGSLVTSMAHVDTFSNHFLFLGFRRGSIAVYRLSDDDPQTSQTTLFRFIENAHGTETVTCLTWNPLSADSAQGHILSVGRNGRLVVHYVDLSTNFVQLVHNLTLPIGPNIESLYFYDDHLLVHGFSSKRWVLYDVTVEEEVMGVETGGAHRSWAFRPSSSSQGGTLVWTRAADMHIYSQIGPNHTVIRSGGHGREIKAVAVSPVGIQRSIPRRIATGAEDTDIKIFKYIDGELVCRKTIRKHTTGIQHLKWSDDGDYLFSSGGCEECT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YD72</accession>
    <name>A0A9W8YD72_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cystathionine gamma-lyase</fullName>
            <ecNumber evidence="4">4.4.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Gamma-cystathionase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CYS3</name>
        <name evidence="11" type="ORF">N0V83_003926</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.1" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YD72"/>
    <dbReference type="OrthoDB" id="3512640at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004123">
        <property type="term" value="F:cystathionine gamma-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019343">
        <property type="term" value="P:cysteine biosynthetic process via cystathionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000043">
        <property type="entry name" value="Cystathionine gamma-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000008">
        <property type="entry name" value="Cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF15">
        <property type="entry name" value="CYSTATHIONINE GAMMA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4372153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="418" mass="44525" checksum="D0A2BB8355532BED" modified="2023-11-08" version="1">MTITEHPIDTPPRAPSPVHGFGTLAVHAGSPHDPVTGAVIEPISLSTTFAQTSVGKPVGEYEYSRSSNPNRDNFERAVAALEHARYALAFSSGSATTANILQSLAAGSHVVSVSDVYGGTHRYFTKVALTHDVKVTFSPSIEIDIAELIRPETKLIWIESPSNPTLTLVDIRKVSTVAHQHGIMVVVDNTFMSPYVQNPLDHGADIVVHSVTKYINGHSDVVMGAAAFNSPELLDRLSFLQNAIGAVPSAFDCWLAHRGLKTLHLRAREASKNATSVAQALEASTHVISVNYPGLPSHAQRAIAIKQHRDGMGGGMLSFRIRGGHEAAERFCQATKIFTLAESLGGVESLVEVPSAMTHGGIPKDQREAAGVFDDLVRISCGIEDEADLVADVLQALEKAVVGPKIKHGLTPNGNGQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDH3</accession>
    <name>A0A9W8YDH3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">O-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_003110</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDH3"/>
    <dbReference type="OrthoDB" id="1606438at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF17">
        <property type="entry name" value="O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005739">
        <property type="entry name" value="O-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="5">
        <location>
            <begin position="66"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="4">
        <location>
            <begin position="239"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4374369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="44823" checksum="992F55FBE39F4E08" modified="2023-11-08" version="1">MASTPPSKTALSLLDNIAELSDGLKHGRPGAREGLLGACANLISELSHPSETMLQLLWAQPTHLNTLWMAVEIKLFQALEASSESGSTVADIAKRCDKDVDPVLVGRMLRHLAAMGTVRETGPDTFAPTATSKAFAEPAYQDSILYIAENFQPVHQALPSYFRERSFKSPDSGVDGPFQHAYNCKGSHYFEYFQKSNPEMGRRFASMMESWSRGRPRWFYEDYYPVKERLISGAEKDAPFLVDVGGGSGHDVEGLREAFERQLPGKLVLQDRPEIIELAKLGAGAEGMPHDFLIEQPVKGARAYFLHSIIQDWNDEVNTQILKAIVPAMKKGYSKVLVNDFVVPNQGAHWAQTCLDWELMASLGARHRTEAEHRTLYEGAGLKITGIWRHPQSLDSLIELELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YDY0</accession>
    <name>A0A9W8YDY0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mtf2-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002216</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4375132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YDY0"/>
    <dbReference type="OrthoDB" id="2444174at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043837">
        <property type="entry name" value="Mtf2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040009">
        <property type="entry name" value="Mtf2/C5D6.12-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39468">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39468:SF1">
        <property type="entry name" value="MTF2-LIKE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19189">
        <property type="entry name" value="Mtf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mtf2-like C-terminal" evidence="2">
        <location>
            <begin position="197"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="498"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="512"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="524"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="536"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4375132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="545" mass="61437" checksum="6C29017F3AE18355" modified="2023-11-08" version="1">MSICSSSIRALSRSRVSPSRTLLPFLYQTATLQQWRPAARRNVTSRSNDRDDIPFEDETLPPAIDNPDPARKTTITGSERAAFEKLYRTFNTRGQPKNDKDHVVELDQIADEYYEDDENNQSQSLDKVFDEVLKGEPRLRASRHDHGRPKQEKKEPDQDAQTFAEGTLSARTKKAQNKRKEAKAEAARIKELKFAERERVDKLLQNAQTDRKLWQILNREVLDQVRKLDLDGTKSGKAAQSSKTGGAPSRKSKPDLPATTDRRILFQNYSHHLITAIQTLRTAFPSSPLPHSILPTIKSLGRSSYALGTTTTLYKHLIRTAWLQQSSYTYIDSLLVDMNNGAIEFDSDILALLDAIIKQHDMARSGRLGREMQMVYGMEQFLEGIRKVRQWRGEVAERLGVVPPDERRVPQPGLVRAVDRWGKHVDPERLRGRREDSGGEADLEKAGTRENIPLVEEQGTGEAEVGGVLPKLETPQHVDAESMREAELLAQELGLLGDARDREAGNSGIATEKNHDDHDPLHEQQQATQDTVDTTTDDKPAKIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YEE7</accession>
    <name>A0A9W8YEE7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_001174</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YEE7"/>
    <dbReference type="OrthoDB" id="294295at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051122">
        <property type="entry name" value="SDR_DHRS6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057571">
        <property type="entry name" value="SDR_PhqE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43477">
        <property type="entry name" value="DIHYDROANTICAPSIN 7-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43477:SF1">
        <property type="entry name" value="DIHYDROANTICAPSIN 7-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23441">
        <property type="entry name" value="SDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4375896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="264" mass="28720" checksum="9F3D96D1CD3B641D" modified="2023-11-08" version="1">MTDQRKYTAKLEGSRVLIIGGTSGLGFTVAEACVEYGALVTVSSSNKDRVQGTMEKLKKSYPSASDRVFGLTVDLSKAETLEDELKTLLEGTMRDLGGKLDHVVFTAGDALAVIKLEDMTMQKILQAGQIRFFAPLLLAKYLPKYLNNTYKSSYTITSGTIAEKPIPNWSAIAPYAGGQHAMVRNLALDLKPIRVNGIGPGVVDTELWRQPKEEKEKLLKQASGRMATGRPGEPEDVAETYLAVLKDWNMSGSMIRSDGGGVLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFD6</accession>
    <name>A0A9W8YFD6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_002671</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFD6"/>
    <dbReference type="OrthoDB" id="303614at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43102:SF2">
        <property type="entry name" value="GAF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43102">
        <property type="entry name" value="SLR1143 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAF" evidence="2">
        <location>
            <begin position="57"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="468" mass="52386" checksum="46E9BEAF27F437C1" modified="2023-11-08" version="1">MEDPLPPLDTPKPAVDWRRERDVFNLYAAFQDIPRIDNLGFSPDTHSFPADYVPRPASDASLAAFAQLAAMRLQAQRSMISLLDGQFQYILAEATPKTSLRFDSPLHKNLDILLGNVRIPRNWGLCERVLDPLALAEGDPGIIIIKDLSQSREHQNRSYVKDGPQFRFYAGVPIRSPNNTIVGSMCIFDGPERDGMLPDDIMYLQDLAATVMDYLATYTLRDQRRRGAERLHGLLSFAEGDSKSVPSQEHDRPPGLSSHESDSVTTSTWDTASTRDTVNTEDNPKNEGILMAEELEQDAPVQPDMNQPRKTSDPPKRRTSDLQDLILPNTARKLFARAAEIMRRSNDMDGVVFLDASVAATGFHGGGKTPTRTSGKQCRILGFATHDRSSLKGDSMPRNMIPDETDFTWLLKKYPHGYSLDCDDGQAQSWTGDGVPLIEPISPVEPWIKLFEWTNQSKNLPAIMRNMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFF1</accession>
    <name>A0A9W8YFF1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">AAA+-type ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">AFG2</name>
        <name evidence="6" type="ORF">N0V83_001720</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFF1"/>
    <dbReference type="OrthoDB" id="27435at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000178">
        <property type="entry name" value="CDC48/VCP homolog, AAA superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002219">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF27">
        <property type="entry name" value="ATPASE FAMILY GENE 2 PROTEIN HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="248"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="510"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="310"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4376436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="745" mass="82420" checksum="016C37EA84D3C295" modified="2023-11-08" version="1">MSTGTAFALRPLERNPPSTPSNALEGAFRVHLSQKEQKNLALTNGDLIRLSTVNGFRGYGVVWTASQTNPGNKPIAKVTDLLREHYELALNDPIFVEKVVDPGKPLKSVEVSLLELSDQATKFSSNEELLYWVQYALVDLEIILPGCNFLVQQKGPRSQPKGARLRVTVQSIEPHPDEKHPCYFDPLKTQVTATTKIQVQSSSSSSPKILRMSSDGIGGLSEQVATVNRSLFFLTNVAPSLPDQHLLGPTAFLLHGPEGTGKSLLLERLANCSWKEVFRVNPESHPKGQAKAISETFENAHQNQPSLILMDNLDRFLEKAESLVARLRLELSNLEGSQVVVAAAARSVYDVDASLRTTSAFKTELELFPPNVRQREDIFRQILGTTRKLPNVNFSTLAERSHGFVGRDIHKLCGLARNRRVREVYDSLKEDQKAAIGDTLEKADFVAQEDFDAVIDQVQPTVLKDSILEVPKVKWIDIAGLDHVRALLEAITIRPFKYPDLDVKFGGPQSRKGVLLYGPPGCAKTLIAQAVATESNQNFLAVKGSELIKMYVGESERAIRDVFRRARAAKPCIIFFDEIDSIGKSREKTQDSGLNVVTTLLNEMDGIEALKDVFIIGATNRPDILDSALIRTGRFDAHIHIGLPTEEARKQILEIHTRKRPLAADVDLNVVAAKTEGSSGADISGLCSVAVELAISEYEKAPDSVPQICMRHFEQALDQHVPHTVKAEAERYKNWRPGKSLSVDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YFZ2</accession>
    <name>A0A9W8YFZ2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">DNA polymerase</fullName>
            <ecNumber evidence="21">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">POL3</name>
        <name evidence="26" type="ORF">N0V83_001380</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="26">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Catalytic component of DNA polymerase delta (DNA polymerase III) which participates in chromosomal DNA replication. Required during synthesis of the lagging DNA strands at the replication fork, binds at/or near replication origins and moves along DNA with the replication fork. Participates in leading strand synthesis during replication initiation and termination. Has 3'-5' proofreading exonuclease activity that corrects errors arising during DNA replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 21">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 21">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="21"/>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YFZ2"/>
    <dbReference type="OrthoDB" id="2414538at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05777">
        <property type="entry name" value="DNA_polB_delta_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05533">
        <property type="entry name" value="POLBc_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.730:FF:000004">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.342.10:FF:000009">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000004">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.730">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025687">
        <property type="entry name" value="Znf-C4pol"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00592">
        <property type="entry name" value="pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF23">
        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14260">
        <property type="entry name" value="zf-C4pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 21" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 21" id="KW-0235">DNA replication</keyword>
    <keyword evidence="17 21" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14 21 26" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15 21" id="KW-0408">Iron</keyword>
    <keyword evidence="16 21" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6 21 26" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18 21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 21 26" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 21" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 21" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="24">
        <location>
            <begin position="245"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="23">
        <location>
            <begin position="540"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="domain" description="C4-type zinc-finger of DNA polymerase delta" evidence="25">
        <location>
            <begin position="1017"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00055225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF14260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAJ4376099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1115" mass="126590" checksum="4BB3B95130A2F087" modified="2023-11-08" version="1">MPGTVTRTPAKRALAEASNTRANVLASPRSAKKLKLDSGSGKSKPPVKTVNGSFSSSQPAPSQFEETLEKMTQELETLKEKNTERDQQWRRPPLPQDFNEMTQKLVFQQIEAEEGVLNGGRTTVKLFGVTEDGHSVLLHVTGFLHYFYVAAPINFHKEDCDSYKVFLESECQKQFNQHSAVINSVQMTMRENILRFQGNQKSPYLKITVNDHKSINRVRTLVQKGHANYKGLWPYREDGILTFDNIAYVLRFMIDTKIAGMSWVEAPAGKYRMINPRDQHSNCQIEAQIHYTDLIAHSSDGEWAKLAPLRILSFDIECAGRKGIFPEANVDPVIQIANVVTRYGEDKPFVRNVFCMDTVSPIVATQILSFQNEGDMLMKWRDFVEEVDPDVIIGYNTSNFDFPYLLDRAKHLKLQRFPYWSRLKTVQSKVESSNFSSKQLGNRDTKTTNTNGRLQLDLLQLIQRDHQLRSYTLNSVCAHFLKEQKEDVHHSMITELYNGDANSRRRLAVYCLKDAYLPQRLLDKLLCLVNYTEMARVTGVPFNYLLARGQQVRFISQLFRKALDHQLIVPDLPNQGDSGDQYEGATVIEPKRGYYKQPVATLDFASLYPSIMQAHNLCYTTWLDKQTVEKLKMKKDEDYIVTPNGDMFCTAKTRKGLLSQILEELLGARKRAKKELAVETDPFKKAVLNGRQLALKVSANSVYGLTGATNGKLPCLAIASSTTSFGRQMIEKTKAEVEAKYNIANGYSHDAEVIYGDTDSVMVKFGTSELKEAMKLGEEAAEYVSSKFVKPIKLEFEKVYFPYLLINKKRYAGLYWTNPNKWDKMDTKGIETVRRDNCRLVQTVIETSLRMLLIDQDPEGAQEFVKETISDLLQNKIDMSNLVITKALTKQEDKEGKGGYANKQAHNELAERMKKRDAGSAPGLGDRVAYVMVRGATGARNFEKSEDPIYVLENNLPIDTRYYLDNQLAKPLGRIFEPILGEKKANSLLTGEHTRSISVAAPTMGGLMKFAKKTQTCMGCKKPLTAAHEKDGAVCENCRPRIGELYYKTLSKVSELEVRFSRLWTQCQRCQGSIHCEVICASRDCPIFYMRMKARKDVEDAGKEIVRFDKDAALW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG34</accession>
    <name>A0A9W8YG34_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">OPT super</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">OPT7</name>
        <name evidence="11" type="ORF">N0V83_001440</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG34"/>
    <dbReference type="OrthoDB" id="9986677at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="294"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="418"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="481"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="506"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="598"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="671"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="721"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="749"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4376159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="796" mass="89953" checksum="8B5100ECACB8C6A8" modified="2023-11-08" version="1">MAELIDDKLIANGRSEELSAASSPVTTKNKLAEFGATPTTTQSVLDHEDNTAIVEEEAHDDPEIAQLPLIVRQLVSLEDDPTLPTITFRYYVLAIIFVVPGAFLSQMSYFRTTYAPYSVFFVQIASHYAGHFLADHLPAWQVRLPFGYGFNLNPAPWSIKEHVLVTLTAASGATYNLAYAPICMAELFYGTKINPGVAVFFMFAIVWVGYGVAAIARTMLLWEPEYIWPQALMQTTLFETFRKQDRHSRLAKRQMVIFFLCLVGMTLWQFLPEFVFPLTSSLAFLCWVAPKNPVANFIGSGLGGMGFMNLSLDWSNINWNGSSILITPFWTQTVLFLAFVFNCWVLLPAAKWGKLGSYKHGLMSNSLLTANGTKYPTLEIINKDFSLNETAYEEHGPVYMGLQNIWATFFNYAKLPSAFVWMATFGYSSLAGTLKKNRQARRARKEAAKQQSTNESTTPNIHHQYTDRLNILMRPYKEVPAWWFLTLFTIGVVIIFAVVGSGHLFIPVWTVFVGLATGILVVIPCGYLYAISNYQVAIGDFNELMYGYMVQTKAGAGHHHPGGPSVYGSIAGDAWYRAQYMLQDQRIGHYMHIPPRTVFFSQIFGTILGIPINYGVIRWILNTKFDYISGNKKDPLNQWTGQRLVSTNALGVQYAIIGPKRLFSQAIFRPLPYAFLVGALLPLVPYALYRTFPKSKLKFHLWNTTIFFSGISVFYGNISTGYFSAFVGGFIAMYWIFRHHFKIWKRYNYIVAAAFDAAFNLNMLLIFLFFGSGKQISMPNWWGNNADTVERCFALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YG91</accession>
    <name>A0A9W8YG91_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HpcH/HpaI aldolase/citrate lyase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_000224</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YG91"/>
    <dbReference type="OrthoDB" id="2326446at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005000">
        <property type="entry name" value="Aldolase/citrate-lyase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050251">
        <property type="entry name" value="HpcH-HpaI_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30502">
        <property type="entry name" value="2-KETO-3-DEOXY-L-RHAMNONATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30502:SF8">
        <property type="entry name" value="SYNTHASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03328">
        <property type="entry name" value="HpcH_HpaI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="HpcH/HpaI aldolase/citrate lyase" evidence="5">
        <location>
            <begin position="154"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="388"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4377399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="408" mass="44248" checksum="6C373D9A4AB06C3A" modified="2023-11-08" version="1">MTAKIGPLSSSIIAVIAVGGIFGLLVIIITTAWLFDLPKRRRRAQHQGIMEPSGKKSLEIAEAEKGSVDVSVTEVSSTNASTNYSSMRPTRPASPEYFKPYSEQKELHANAPYRSSMLTYPGNLRKAFKDAQADASKTLMGVAHGIPSTFVTKLIATTKPDFIFIDVEHGMFNRLELHDAIHAAQHHSEGKTLVIVRVPKHDEVSLTTALDAGAAGIIIPHTESAEEVKKFIKEMYYGPKGQRSFSPWTFTPGLTTSLYPNDPYNVATSNNHVCIIPQIESVKGVENAEEITAVEGVDAVMFGPGDYLIDAGLDLGRVMAGDPQPEFLEAMGKFGAAAAKNNVPVFGGAMSLEMIPMLIETGSRGIVVQFDVWGFTRLMHESLETARGHAKQFEGNPKAIPNGQPKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YGG4</accession>
    <name>A0A9W8YGG4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_000297</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YGG4"/>
    <dbReference type="OrthoDB" id="77989at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="113"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="151"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="176"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4377472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="527" mass="57076" checksum="F3967246D3E69986" modified="2023-11-08" version="1">MATSRDAPNPLRPYYIPPSIGLPADVPHNQTSSPPRYGSSSSMKPSLGSQARDMLSDLDYDSYFPNNDPTIAGHAKKLLDQALWNYTSVLLAQPFEVAKTILQVREATGQLPGLKIGADGVGKRSRPESFASGKFEDYPSDEESDEDSPGYFTSTAPQIRQPNDYSPSASPSLHSPQKHRRRERSRSRSATPTRPPPATVRKLDLRRADSLLEVIAQLWQKESAWGVWKGTNCTFIYNFLLKTTESWFRSAISALLNVPDPGLIGNAVSGIGGLDILDSPSPLMSLGVAVAATAIAATILAPLDMVRTRLIVTPISSPPRSIYPCLALLPSFSISPSLLPATLLHACIPTIISTSTPLFLRSTLSIDPILTPTAYSFSTFFSSTAELFIKLPLETVLRRGQVAALQDHENKRLSSEYRTLPTHSKSPVYGMEESDLSIRSFKTIVEPGPYKGVLGTMWYVAREEGVTVIGPSPSKAASAKQMGFARPTRTRKGQGVHGLWRGWRVGVWGLVGIWGAAALGGGGGGEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YH89</accession>
    <name>A0A9W8YH89_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000161</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YH89"/>
    <dbReference type="OrthoDB" id="1669814at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008667">
        <property type="term" value="F:2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019290">
        <property type="term" value="P:siderophore biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003560">
        <property type="entry name" value="DHB_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43180">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43180:SF66">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01397">
        <property type="entry name" value="DHBDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="148" mass="15364" checksum="5C504A60F28C98BF" modified="2023-11-08" version="1">MSKFSGKVIAITGAASGMGLATARLLASQGAHISLADINSDAAKAAAESLPGGLDEHMYTSVDVRDAKVVNAWIQATKERFGKLDGAVNMAGIIAKAKPITDVTDEDWDQMFAVNTRGVFNCLKAQVKAMDGGEGCSIVGPNASELHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHD6</accession>
    <name>A0A9W8YHD6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NDT80 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_000487</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHD6"/>
    <dbReference type="OrthoDB" id="4117572at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1390:FF:000007">
        <property type="entry name" value="p53-like transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1390">
        <property type="entry name" value="NDT80 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052605">
        <property type="entry name" value="Fungal_trans_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024061">
        <property type="entry name" value="NDT80_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037141">
        <property type="entry name" value="NDT80_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35144">
        <property type="entry name" value="MEIOSIS-SPECIFIC TRANSCRIPTION FACTOR NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35144:SF1">
        <property type="entry name" value="PROTEIN PACG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05224">
        <property type="entry name" value="NDT80_PhoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51517">
        <property type="entry name" value="NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NDT80" evidence="4">
        <location>
            <begin position="82"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="NDT80" evidence="2">
        <location>
            <begin position="82"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="308"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="394"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="334"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="344"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="430"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4377659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="532" mass="58835" checksum="D0750485E522EA51" modified="2023-11-08" version="1">MSYLATPMPLQTVTGPDQQLALPMVPMDQYDQRSTYEADTFAGSEDAFSSYHLHQPPPPLKRFSSAYEDPFSDVQSGYEQSIHPHEQSGMPESPSIESNNKLLSFSLPSVPYTILTYAMQRVSISMAAQLHGMFFLAESPWATAGDVQAPPTELTCYRRNLFQITGTVTLPRSLQFLMTDQGDQIPILGQELTIAATESLEGNPVKIISVPWKTPATSNAPVEDKTEKEPPTYPLDLSTGQDMDSEYVSFPISWKRLQFRIATANNGRRKELQQHFVVRLKVVATLATGAKVPICEIHSGAIIVRGRSPRNFQSRKDMPISGGTASARKAHLPQQPSRTPTGDTNHTNQTTPNQAQNQNIATTKPMDMLQMPFDFNPNDVPVSPDFLDWKIPGGAMTAIPPDPPSSQIPHATPYAHSTPEISRSVPPPQRTSIAPVTLSLTDDEPKKAPSPSNPHQKRITPVRPPSFSIGVINSPDESADLLYEYFPLGLDDWMPPVDAVYRPHVVHHTNLPQDPKALAVKSRSKRYFSDAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHG7</accession>
    <name>A0A9W8YHG7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_000842</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHG7"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4378012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="76" mass="8953" checksum="1ED99AE196426559" modified="2023-11-08" version="1">MASSGHFRWLTEDWRNESRYLEPNGRHVFVEISETDGDITFNKDNKHDDDLPDLCKESRAKMGDSDWSLRLYVCCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YHZ1</accession>
    <name>A0A9W8YHZ1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Threonine/serine exporter-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_000876</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ThrE exporter (TC 2.A.79) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YHZ1"/>
    <dbReference type="OrthoDB" id="413008at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010619">
        <property type="entry name" value="ThrE-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051361">
        <property type="entry name" value="ThrE/Ser_Exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31082">
        <property type="entry name" value="PHEROMONE-REGULATED MEMBRANE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31082:SF4">
        <property type="entry name" value="PHEROMONE-REGULATED MEMBRANE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06738">
        <property type="entry name" value="ThrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="548"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="573"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="628"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="666"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="708"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="845"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="911"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="domain" description="Threonine/serine exporter-like N-terminal" evidence="4">
        <location>
            <begin position="442"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="295"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="360"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4378046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="946" mass="103798" checksum="15A96A5051BE6982" modified="2023-11-08" version="1">MDSFQDPGESSLSQFDPDDHMSSTPGRKRVGFTSEIPNVRPLSTHSPPDEDTGSAGHNTPLQRSDVSHDDLQKMRESLRLALGEEYPHDPSAIAPPPKEVQKPRPAIRKGPRSPPEFYLNDQPNPFEDDATETPSLHADVKERSGLAAQRRAAKLSKSVGTFSAPVSRRGSAELLATPPVELRDLARTYETVGDTTDDDEEDPQFLKRASMLLRQHTVHAAGPAISSEDLYHVDAALRSGQVTPDAIEIEDEHVSRPFKYRTGVLGTLLKASNAAQAPASGRPGHVRNLSASTFSGASTAANSPNPSPPASGYTTPRNGRSWFNRHHHTHSATSISQLVGSSTHSFATAAQKDLGLELDEQYKKSRPGMGKRSRSDESILPFKKSAKRRDNEIKIKIHFLLQTGLTQPLRDEFFYDLSKVYSGRSLTIVMNRAGTLARQNYLRKLCKALMMYGAPTHRLEEYLNMSARVLEIEAQFLYMPGCMIIAFDDSSVHTSEVKLVRTSQGVDLGKLRDVHEIYKDVVHDRINVEEATPRLEAIIGRKDKFRRWLRVFMYGLASATVGPFAFQARFIDLPFCFLLGCIIGWLQLIVAPGNDLISNVFEIGASIITSFAARALGSIKDHDGKEIFCFSAMAQSSIALILPGFMVLCASLELQSKHIVAGSVRMVYAIIYSLFLGFGITIGTVLYGMMDKNATSNTSCSDPMSSSFYYLFVPAFAICLMIVNQAKYKQMPSMTVIAFIGWVVNFHSSKYFKSNAQVSNTLGALAIGIAANAHARFGRHLENKSLDFWENTLRPRFVKVRKSWRRKHHGPHHPVPKLERSEYDPAYSSSRASSISEFVPHTRKIGYGLAAAAMLPAIFVQVPSGLSVQGSLVSGLTSADQIVRNSTGNATTLSSGDVGTSTSLNSIALDVGFSVVQIAIGITVGLFLAALIVYPLGKRRSGLFSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YIV4</accession>
    <name>A0A9W8YIV4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Scramblase-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_000165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4377340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YIV4"/>
    <dbReference type="OrthoDB" id="191150at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF9">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="388"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="396"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="450"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4377340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="50542" checksum="979D624D88456856" modified="2023-11-08" version="1">MPEDPHAVLKETHPAMRLLDNSAIVVQRQLEMMNVLMGFEQANRYVIMDPHGNHIGYLAERDHGVGSAVARQMFKTHRSFTTHVFDRDEKEILRFHRPFSWINSRIRVYDAVGDGTAYTSSTSLQGTSAASIVNQSSANVSGLALKDMRIIGSSEQEWAPLRRKYNMFLARNLGDDAAASGTPHIASGDLPLSSSKAVAVVEGDTREVGMLQFARVDEAFLSWDFSLMSEDGRLIGSVNRNFSGFAREIFTDTGVYALRMDAAGLVNEPSHLVSQTGERSKSSLERYPGMTLDQRAVMLATAVSIDFDYFSRHSGSGGMMPMWMPMWWGGGEAAGGAAAGEAGAAGAGAVGAAGESGITGEAGAAARGLGSAEGVAAGAGTMAGYEAMQRGREARQQQQEQSDDASPQAADPYQANPDQQPGPQQGQEDVWGQAQDPWAQDNNQEQDFWGGQGGQGGGGDGGGGGGDSDWGDWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YJQ2</accession>
    <name>A0A9W8YJQ2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_001027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YJQ2"/>
    <dbReference type="OrthoDB" id="3658431at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4378193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="187" mass="21847" checksum="5A1700C4E44A71AA" modified="2023-11-08" version="1">MPFIWADKQFAWNKNIDNPMRCIIQNDVFAGRRNQLRQTYFEIMSDSMAFMCGGKVAVMDRNIRRGRTGRVKQAGIWERKEFPRLKMDADHKWEDGNGNNRVATRIDAIGENLASVPDTLIWWKSWWYRGGPDWPNEFTKPFKRRGVEGEAEVNGTEAVGLEETQKLHKRDFDTSIFDEGGDLEIDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YKR5</accession>
    <name>A0A9W8YKR5_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_000924</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000001">
        <property type="protein sequence ID" value="KAJ4378092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YKR5"/>
    <dbReference type="OrthoDB" id="5334244at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4378092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="202" mass="21622" checksum="FFBABB1612265200" modified="2023-11-08" version="1">MITKELNIMSFLRPRSMLRSVALRPAALSAAPRARLQVRFATGDYGSPEGNPAGEKAQKQGQNASENLEHPGPPPPKVAEGKSSSSPNSDDKSSGDNSSPKSSSSSSSKGSSSGKREFSTSTRRWAGEEPAVKQGQANKPSKSDQKKAEPKILNENPPAKGEEAEDVKKHNKEMDNRAEQAHEKVANEDAAKDKVPSGFWKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CGL1</accession>
    <name>A0A9W9CGL1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Checkpoint forkhead associated with RING domains-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">N0V83_010491</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DMA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CGL1"/>
    <dbReference type="OrthoDB" id="687730at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097271">
        <property type="term" value="P:protein localization to bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090337">
        <property type="term" value="P:regulation of formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000921">
        <property type="term" value="P:septin ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000426">
        <property type="entry name" value="DMA1p Ubiquitin-protein ligase (E3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000030">
        <property type="entry name" value="FHA domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15067:SF7">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE DMA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15067">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17123">
        <property type="entry name" value="zf-RING_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FHA" evidence="15">
        <location>
            <begin position="83"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="226"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="387"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="395"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="436"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4362398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="456" mass="49134" checksum="C693A81ACA53C10A" modified="2023-11-08" version="1">MTRTRAETAAPQLTVRNNTGPTPTSTPGPSASVEPSDSSAEGAAASKHLPSIRFIPHQDVRAGRPSLQFPTITRTLPDESAVIRVGRYSERDNIPEVPSHAPSAAAVGFKSKVVSRKHCELWCKDGSWYIKDVKSSSGTFLNHIRLSQPNIESKPFRIKDGDIIQLGIDFRGGEEMIFRCVKIRVECNRGWQQGLNTFNKQTHQRLRNLAKSKKEGDAASTHTSECAICLMSIAPCQSLFVAPCSHVWHYKCIRPILNGPTWPNFLCPNCRAVADLEEDVDDMGEFEDWGEEADPVNGDSTAQVESEVGDRHVTPRASIAPVNGNNTDGGVGLSDLQQAISNISITDSLNGIANQPVPQTPDRHLTPVTSSVTQPVTINVSGSNEFSGLSPLYPPHHDGLTPDRVHDGPMTPRNDAGPFVLDGSAGRAAGSRMRDASPGSDSGSSGAPSLPPVRFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CGM0</accession>
    <name>A0A9W9CGM0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CGM0"/>
    <dbReference type="OrthoDB" id="5355007at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="144" mass="16021" checksum="264CEF2038B469F0" modified="2023-11-08" version="1">MSDKKPVSMTTDQAQSGISQELRNNIYSALLSYGGIRNIESTLDEVLRREGFKDELRRYITYLFRSGQATSAEQARDLAMEKIREAMQSSKTNGTTNGTNGTSNGTVDGSEDYNLRIPHEAIKAGTRSVQEELDKVCDITCEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHF0</accession>
    <name>A0A9W9CHF0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RA-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_010522</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHF0"/>
    <dbReference type="OrthoDB" id="445896at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01786">
        <property type="entry name" value="RA_STE50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09533">
        <property type="entry name" value="SAM_Ste50-like_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin-like_domsf"/>
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        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 15"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="67"/>
            <end position="130"/>
        </location>
    </feature>
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        <location>
            <begin position="409"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="210"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="287"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="486"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="166"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="318"/>
            <end position="329"/>
        </location>
    </feature>
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        <location>
            <begin position="332"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="500"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4362429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="522" mass="57132" checksum="0A7DBD6729616352" modified="2023-11-08" version="1">MAYTTKYHEDSDADDEFERQGIHSPTLPAEYESSPTSSGPLSTEHTPTTFTHSRDSKSSPTGHIMEWSADQAADFIADLGLEQYADTFVDEGITGDVLAALQHAELKEMGVGSVGHRLTILKAVYEIKIRQNVPMEPDDYVPLSADTSAQEAPPTQDDFAKVIRVVQARDERIHTAESELRHLREDLSRVMDENKRLREEILPLVRMLKDSQQPLPTPHEHTVTSPPATEQRSGGSSLSRKFSTKKLFLGSAPKNPSPTIQEHRALVDHSSLDPSAAAVAASSHLTASLGGGPQMSPNSIAQPSPTSPAYTQGQSRQYNRDVARHDHDSTPGWGQTNTWASDSTAVSDRNRDPRATPAPLSSRPMRSNAVPTPNPDDRDPPLSGGSSTLNVPTSNPASTPSTNPQVEIFKSFRVSIDDPCHKVLPVALKKYNITADWRQYALYIVHGDQERCLGLNERPLILFKQLDKEGRKPMFMLRKHAAPAEGHVSTVGGNDVRPVNPQNESFGWGNQNRGTPLPGGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CHI4</accession>
    <name>A0A9W9CHI4_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 4B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TIF3</name>
        <name evidence="5" type="ORF">N0V83_010464</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CHI4"/>
    <dbReference type="OrthoDB" id="48651at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000356">
        <property type="entry name" value="Translation initiation factor 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF11">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="82"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="150"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="158"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="229"/>
            <end position="242"/>
        </location>
    </feature>
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        <location>
            <begin position="255"/>
            <end position="271"/>
        </location>
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            <begin position="286"/>
            <end position="298"/>
        </location>
    </feature>
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        <location>
            <begin position="318"/>
            <end position="334"/>
        </location>
    </feature>
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            <begin position="357"/>
            <end position="366"/>
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    </feature>
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        <location>
            <begin position="383"/>
            <end position="422"/>
        </location>
    </feature>
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            <begin position="431"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="456"/>
            <end position="466"/>
        </location>
    </feature>
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        <location>
            <begin position="470"/>
            <end position="491"/>
        </location>
    </feature>
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        <location>
            <begin position="497"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4362371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="540" mass="58323" checksum="8F8D5783958BA2B6" modified="2023-11-08" version="1">MAPKKKEQQKMSLGDFLGDQSLGSWADEMEDAPMPGGARAGYGGDRPSYGSGGGFGSDRGASFADRGFAVREQLPLPSKPPYTAHLGNLSFDATEGDVNDFFAACEVTNVRIVEDKLDRKPKGFGYVEFGSVEGLKKALDLSGTQFQGRNVRVSVAEPPKDRPEARDITDWTRKGPLPDLPGQRRTSDRGGFGDRGGFGSNRDRNFDAGSDAGSERGERRRGPPPFSGDGKERDFGNWERKGPLSPVPSAGPPREGGRQRAFEGGPREGRRQSPAWGEGQGSEGSRGPRREFQERPQYDRQPTAAEQDNQWRTKMRPDAPAQSPTATPDASTPSSPAPQPAAPAGRPRLNLAKRTVSEAQPAQEATPSDKPNPFGAARPIDTAAREKEIEEKRQLAIRQKKEADDKAREEKKAKEAAEKAASKDSTPASPTEKKKEEGGEEKPKATFEVLQRVEESESAPQEEDANGEIVDDKGVKPQEVVRDPPKSEGSWRQKSSTPAAPADTTTETLDEDGWSTVTKPVKSAKNNRRGGGAPARAIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CI25</accession>
    <name>A0A9W9CI25_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009321</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CI25"/>
    <dbReference type="OrthoDB" id="10646376at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="203" mass="22202" checksum="379579BD336F070E" modified="2023-11-08" version="1">MVSATPSAPEPSTVHILVTSTITVFPSQTAPPLNSSNMAQQWTWPQAAPANLHKEEVDTEDLNTHLQTPTVTAMPTHYTLPPSVSQGSNPNDRSNPRQTLSHEVIAACVIGLFAGIGFVGLAVTFFCRTCCATRDVIKSGKAKKSARCSDIEMGGGLTREDLRAHSMPYSKRNSGIQAVKKPQEAYFVEQPLHWPPEEMQRYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIB1</accession>
    <name>A0A9W9CIB1_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CENP-V/GFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_009445</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfa family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4363991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIB1"/>
    <dbReference type="OrthoDB" id="9985472at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1590.10">
        <property type="entry name" value="glutathione-dependent formaldehyde- activating enzyme (gfa)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006913">
        <property type="entry name" value="CENP-V/GFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33337:SF43">
        <property type="entry name" value="CENP-V_GFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33337">
        <property type="entry name" value="GFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04828">
        <property type="entry name" value="GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51891">
        <property type="entry name" value="CENP_V_GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CENP-V/GFA" evidence="5">
        <location>
            <begin position="21"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4363991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="167" mass="18438" checksum="D426FA00F86C1142" modified="2023-11-08" version="1">MANDTYKFTGDPNSHDISKFKDGISATCLCGSIRVTINDNELFTKKRGHLCHCANCRKVAGSYVSSNLAIEKEKVNVDDPSGVLKKYKDYATASGKCVERCFCGTCGNPIMSIAEIYPTMVIVKMGMFPRIPDPECESFALHRHVWQGKHGDLPQFEIARGGKKLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIE7</accession>
    <name>A0A9W9CIE7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009508</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIE7"/>
    <dbReference type="OrthoDB" id="19657at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08983">
        <property type="entry name" value="GH43_Bt3655-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050727">
        <property type="entry name" value="GH43_arabinanases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43301">
        <property type="entry name" value="ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43301:SF8">
        <property type="entry name" value="ARABINOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4364053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="204" mass="22439" checksum="AF35D65C333B16DE" modified="2023-11-08" version="1">MVHDCDRVEGATAGMVWAPSAVWDEATQQFYVFWASRLYSSSDTGHTGTAGLDRIRYATTKDFSTFSAPQDYVAPSGTPVIDQEILYLGTAGHYARFIKNETVNQVYQEMTTGGLFGSWSRIPGYVVSGSPWEGPASYADNTVAGKYYLLLDNYTEYVPYQTTNIKSPNWSAANTSSFPQGLKHGSVTPVTQGEYNALKSKYPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CIN0</accession>
    <name>A0A9W9CIN0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAD(P)-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008974</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CIN0"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43963">
        <property type="entry name" value="CARBONYL REDUCTASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43963:SF6">
        <property type="entry name" value="CHAIN DEHYDROGENASE FAMILY PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G15350)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4364381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="296" mass="31998" checksum="D7562BE3F292C261" modified="2023-11-08" version="1">MANNLVVVVTGSNRGVGKGIVELLAKQKLERPLTIYATSRSGENANIKASSPNEIRYQKLDTKDSASIQSFFNIALKEQTALDVLINNAAVSNDYRETPDYAAETVQTNYGGTRDMCKAFLAQPNLKPGSRIVNVTSGYNLLKTYGQDIQNRFRAVTEISDLDAMADDYLKVVKLGQEAQQEAGWGSGARSYKVSKALNNAVTILLAKQHPEVLINSCCPGWTDTAMGNQAQGGTPPKTPQEGARVPVRLAIGDLGPKGDEDGALGHDTEKISGHFFENVNIMAPGWGKAKLWLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKN2</accession>
    <name>A0A9W9CKN2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006349</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4367994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKN2"/>
    <dbReference type="OrthoDB" id="1911848at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1020">
        <property type="entry name" value="Prion-inhibition and propagation, HeLo domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029498">
        <property type="entry name" value="HeLo_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038305">
        <property type="entry name" value="HeLo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37542">
        <property type="entry name" value="HELO DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37542:SF1">
        <property type="entry name" value="PRION-INHIBITION AND PROPAGATION HELO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14479">
        <property type="entry name" value="HeLo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="248"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="577" mass="65254" checksum="17557EFA1F8C26FA" modified="2023-11-08" version="1">MAQFNMDIGAGTVQTLGFAGQIFTGCLNGFQAFQKAQDLGRDALQLQITLEWTRSRLDMWGTLWGLERQEHLKDIRFRQFGMMALNHLVYINYCLEDFKTMDDIFPTLGDASKYASTPAVSLARLSKSAEVTPDEMDALRYKMEQLQVNAAGKEKVKWALQDGKALRMAETVKAMVEDLYTQFPPPAAETNAIVKNAYAASDSLDTLDDAANSSSSGPELASLCKFKAENIRLHLEKRSDKLQSTDPERRSDVLAETQMHKRADGTQNENRWLGRYREDGVSYSIPVLTEHKTVQGNVMTTSPIDNRIRNVARLLSMESKPAELRTLNCLGVTIGHSVPRTYRFIYELKSKTFFTLADCLRTPTEKNLNGLHRDKWPLEQKFNLAKQIARAVQYFHLAGWLHKGLRSSNIIFCADNEALIDLSEPYIIGFDYSRGEFARYETETVETTDDVEIAANAFRHPDTQGPPTDRSQFERKFDIYSLGMVLLDIGSKRSVPNLKEKFNKMPDRAPWAAEGFRDWLLVDAIDSNVTGLAARMGSKYADVVKTCLRGKFAESHKGDANVAFYLEILRQLQLCRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKS3</accession>
    <name>A0A9W9CKS3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphatidylinositol-specific phospholipase C X domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKS3"/>
    <dbReference type="OrthoDB" id="1046782at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08586">
        <property type="entry name" value="PI-PLCc_BcPLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF113">
        <property type="entry name" value="SI:DKEY-266F7.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatidylinositol-specific phospholipase C X" evidence="1">
        <location>
            <begin position="166"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4367455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="524" mass="58329" checksum="B8E895E5DD314CC2" modified="2023-11-08" version="1">MPSQLPVRNLTSTAITIRRIERFQDPNTLQSKATGYFFGSNNTSLAAPTSPQLGEHARSFNHQDLDVTLAPFETYNLRQSEAQDETASILSSTTTRITIETLQGERYRIDVNPSYSQRSSQPLIALSPNPSTSYTALFHPSNPVPHLTLHANHLPNYAEWMAKLPDTLPLSAISIPGTHNSHTHYRALPSVRCQVVNIKTQLDNGIRFLDIRVQPVHATDTTKKYLYLVHGAFPISLTGPKYLEPILQTCYAFLSSHPTETILISLKREGVGSSTDEHLARILEEHYFKPNASFWHTADSKLPYLGSVRGKLVLIRRYKTPPPPLSSPEGEEQEVSKQYSGAGTGLDATAWPWNATHALFPTPSTPSSSSLFCLQDYCEILVPSLITQKIQYSNDHLARAAAATHHIPGFNTDTLNPVPPGPLYLNFLSASNFWKRACWPEMIAKSVNRGVEEWVCRGLYLEEESGAERGREDVVVVRKKKKSGDGGTGVVVMDCVGENGDWEFVRLIVGMNMGVLMKMEELKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKT3</accession>
    <name>A0A9W9CKT3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_007189</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKT3"/>
    <dbReference type="OrthoDB" id="10250504at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041178">
        <property type="entry name" value="RPA43_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF5">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17875">
        <property type="entry name" value="RPA43_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RPA43 OB" evidence="7">
        <location>
            <begin position="122"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="55"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="232"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="58"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="239"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4367604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="262" mass="29293" checksum="B68A28E8890FFEFA" modified="2023-11-08" version="1">MAPRISQYVSLSPSSLSTPLPALCASVLSPLLLSYFPPAKGIVLAYENVQLSATAPASQSHVSKSKSKSQSQPKQRRSQEDQDEDEEENAKEARPEPLLLRHVDEYSAPFLWATASFLVWRPRRNAYITGRITHQSKTHITLAHLNTFPVSILKEHLPSSWVWHSTEESGKMRKGWDGRIADEGGWWVDENDGEKVEGEIKVRIRDFDGRMDGRGKGKGFLRIEGSLLSEAEEKERVKKGAKGKQKASSKGKQKNGEAMEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CKV5</accession>
    <name>A0A9W9CKV5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Complex I-ESSS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">NADH-ubiquinone oxidoreductase ESSS subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">N0V83_006545</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Complex I is composed of 45 different subunits. Interacts with BCAP31.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFB11 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CKV5"/>
    <dbReference type="OrthoDB" id="2147978at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019329">
        <property type="entry name" value="NADH_UbQ_OxRdtase_ESSS_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40637">
        <property type="entry name" value="ESSS SUBUNIT OF NADH:UBIQUINONE OXIDOREDUCTASE (COMPLEX I) PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40637:SF1">
        <property type="entry name" value="ESSS SUBUNIT OF NADH:UBIQUINONE OXIDOREDUCTASE (COMPLEX I) PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10183">
        <property type="entry name" value="ESSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ4368189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="123" mass="13412" checksum="F45B90B74BED925B" modified="2023-11-08" version="1">MQSLRAAASGSRALATCTARHSTSLRSLTGARAFSQSTAVRSGGHDSHYDPPSGWLFGVKPGEQYEKEGWENVMYWGFGGACAFGVIAYAFKPDTSIQTWALEEARRRLEAEGILPDPDNKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CL31</accession>
    <name>A0A9W9CL31_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006517</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CL31"/>
    <dbReference type="OrthoDB" id="2544694at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11578">
        <property type="entry name" value="DUF3237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4368161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="169" mass="17853" checksum="19C1C6E7077B6BEB" modified="2023-11-08" version="1">MSGFPSLQPAFTVRVSIDAPMPVGGQAGSSLVIVPMVSGTVKSEPGFEPKLDGELHGVGYDYIHNDASGENMRLDVRSQVKTSDGTILAMYYKGTVALSSGVQAILSGSPDAKTTGYGDSFVAFTFETGSQAYKELQNGTYVAAGHFVKEAGQQGPIVEYKVSKVVYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLB7</accession>
    <name>A0A9W9CLB7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Kinetochore protein SPC25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SPC25</name>
        <name evidence="12" type="ORF">N0V83_006519</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Chromosome</location>
            <location evidence="9">Centromere</location>
            <location evidence="9">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the SPC25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLB7"/>
    <dbReference type="OrthoDB" id="4056921at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23784">
        <property type="entry name" value="RWD_Spc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.457.50:FF:000001">
        <property type="entry name" value="Probable kinetochore protein spc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.50">
        <property type="entry name" value="Chromosome segregation protein Spc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045143">
        <property type="entry name" value="Spc25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013255">
        <property type="entry name" value="Spc25_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14281:SF0">
        <property type="entry name" value="KINETOCHORE PROTEIN SPC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14281">
        <property type="entry name" value="KINETOCHORE PROTEIN SPC25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08234">
        <property type="entry name" value="Spindle_Spc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 9" id="KW-0137">Centromere</keyword>
    <keyword evidence="2 9" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="4 9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromosome segregation protein Spc25 C-terminal" evidence="11">
        <location>
            <begin position="180"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4368163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="254" mass="30293" checksum="0BC1081816269980" modified="2023-11-08" version="1">MTTFEPSLSTTMRASSHDAPSMADQLPSINFGFDDLRSRMTQFTARFDAFIENGRRRVLEERNQFRMNVAELHEDQRMKKRDIEILELKQTQHSQTVSKESQETTEMQEAIATLTLQRDERLAHRDTLRSQIAEIQKSISARREAQLKHRRYLDGQSRYNEPELDFWESYLGMRIEGLGKDDRLKFVYTNVDEREWDREAWFELDTSERDYKVLEVRPKVEREEVERVVERLNETRDLASFLKGMRELFVEACK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLG0</accession>
    <name>A0A9W9CLG0_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_006789</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLG0"/>
    <dbReference type="OrthoDB" id="2985014at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF3">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4368432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="96" mass="10899" checksum="A8AF8F9F25EA23CE" modified="2023-11-08" version="1">MVKEKHDVDYVEKAYDVPIPPEGGEHPEQDWTPEEEKAVVKKADWRVFPMLCFVFGLSLLDRTNISAAYIAGLSVDLKLGIENRYNIALLVFFIGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CLJ2</accession>
    <name>A0A9W9CLJ2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006745</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CLJ2"/>
    <dbReference type="OrthoDB" id="5342184at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021986">
        <property type="entry name" value="Spherulin4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35040:SF7">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12138">
        <property type="entry name" value="Spherulin4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="427"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="716" mass="80981" checksum="49A4679C0FF67E82" modified="2023-11-08" version="1">MDQRQLAIQRSLAPEPIRGHWWRAAWATYTRLPRELRDMVYQHVWSDEANRTMIPKHSRPAFVVPLIVGEDFAREAAEWYYEHGCLGRPVLLKNLKDFLVDDYFLLGVTPDITTMRKLAVRVEFELLVPTSVLVADVEARFNHLFRLRLMPGFNITIVLDIKFEERRIPNSVLALCHYIQRVIQAFEHKGCKVMVELDLVKIETQYDYFNGPYARGLNPASYHYLSMMTPVASPSNPLYHSNPINRWQRIESSPSLLYRIIDGEAAKDMLASTAYEWEQFLWSDKDLLRNVTPEARNANVPPSQPLSQNTSRTSLSTYKAQSRMSTSSRRNISSPYSLAIPPKNNRTSTSTSAISEYQQAKPTLAGTKSVERLRVPTPPKKAVLVSEKPIQPCVSNDKAISTTSSHHGYASPYIPAPKKKLEMNDRVLAFAVTLGLVLLLAIIIPLGIIIPQKLIKPLPINAIVPFYINPEQSGSWGRLYDAIVRHPDTNFTIIINPDSGPGTTVWPPAPYIDAIKTLNVHLNVKTLGYIDTKGGTRDNATIRSEIDIYAGWANVSDKLTLNGIYFDHTPWNYTEHAAAYLRNISAAVRRSDGGFGDERAWVVYNPGRVPFEDNTQRGHSKGLMPYKPDVTVVFEGVYDDMPRKNKLQDLLGSSKDDRAGRAMLVNSVPKDLGLGGLRKIVDDVRRDVGWLFVTDLSDDVYAGYGSIWEEWLNVMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMN5</accession>
    <name>A0A9W9CMN5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PRE1</name>
        <name evidence="6" type="ORF">N0V83_005080</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMN5"/>
    <dbReference type="OrthoDB" id="268428at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03758">
        <property type="entry name" value="proteasome_beta_type_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000008">
        <property type="entry name" value="Proteasome subunit beta type-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035206">
        <property type="entry name" value="Proteasome_beta2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF2">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 5 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4370559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="192" mass="21658" checksum="AFE3D0A6F101901B" modified="2023-11-08" version="1">MRGATILKASDDKTRVLNTHTLMTFSGEAGDTGMTEDILGDLMQFAEYIQANVQLYSMRNNMELSPAATANFVRGELARALRSRKPYTVNLLLGGYDTIADKPTLYWIDYLASQAALPYAAHGYAQYYCLSLLDKHHHPDIDFDQGMKILRMCTEELKRRLPIDFKGVLVKVITKDGVRDVDYEDDVKVSIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CMP7</accession>
    <name>A0A9W9CMP7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">D-malate dehydrogenase (decarboxylating)</fullName>
            <ecNumber evidence="4">1.1.1.83</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_004675</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(R)-malate + NAD(+) = pyruvate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:18365"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15588"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.83"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.83" evidence="4"/>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CMP7"/>
    <dbReference type="OrthoDB" id="10261637at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046553">
        <property type="term" value="F:D-malate dehydrogenase (decarboxylating) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050501">
        <property type="entry name" value="ICDH/IPMDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011829">
        <property type="entry name" value="TTC_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02089">
        <property type="entry name" value="TTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43275">
        <property type="entry name" value="D-MALATE DEHYDROGENASE [DECARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43275:SF1">
        <property type="entry name" value="D-MALATE DEHYDROGENASE [DECARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="11">
        <location>
            <begin position="11"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4371458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="368" mass="40035" checksum="FEBD1C06EFB1AA76" modified="2023-11-08" version="1">MSPIATQRVHSIATIGADGIGPEVIDAGVQVLKKLAELDGTFSLDFQDYDWSSETYKKTGQYIPDGGLEKLKKHDAILFGAVGAPNVPDHISLWGLRLAICQTFQQYANVRPTKIFRGTSSPLRKAEHGDLDWVIVRENSEGEYAGQGGRSHRGQPWETATEVSIFTRHGVERLIRFAFETAQKRPRKFLTVVTKSNAQRNGMVMWDEIAAEVAKDFPDVKTDKMLVDAMTCRMVLDPKSLDTIVATNLHADILSDLAAALAGSIGIAPTSNLDPTRQNPSMFEPIHGSAFDITGKGIANPVGTFWTASEMLAWLGEEQAAKKLMDSVEAITERGIATRDLGGKAGTQEVTDAVCAELEKVYGTSASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CN61</accession>
    <name>A0A9W9CN61_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_004276</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000006">
        <property type="protein sequence ID" value="KAJ4372502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CN61"/>
    <dbReference type="OrthoDB" id="786951at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025239">
        <property type="entry name" value="DUF4187"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039249">
        <property type="entry name" value="GPATCH11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21032">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21032:SF0">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13821">
        <property type="entry name" value="DUF4187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01173">
        <property type="entry name" value="DUF4187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="34"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="274" mass="31596" checksum="7D0B71C1BEF2B360" modified="2023-11-08" version="1">MKMGEARGRQKSKAEREAEEEAAREASLATALPETNKGFKMMAKFGFKQGDALGKSEGARKEPIRVDVKDDRSGIGLESEKKRKFREQFAEAERLAKRSKEEEGDYLEMRRQEQKEKKLERDLDSAQRTAERLFEKAAEDSSTLKDGERPLEDINVLWRGRARNRVEAKREKQLRRELNDSIASRLPTLANEDDDDDSTVAEGRDLAPFYTTLENDLVDEDPELVEFEALPVAERLQKLVTFLREEYQYCIYCGYQYPDAKMDDCPGPTEEDHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNA6</accession>
    <name>A0A9W9CNA6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PLC-like phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_004770</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNA6"/>
    <dbReference type="OrthoDB" id="7984201at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF140">
        <property type="entry name" value="PLC-LIKE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26146">
        <property type="entry name" value="PI-PLC_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4371551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="34699" checksum="84997513939FC0C0" modified="2023-11-08" version="1">MVVAHNSPFVKAHNAASNQVYPVTNQLNDGIRGLQFETQKPNSTSAIRLCHTSCDLLDVGTLESYLTTVTSWLESNPYEVIAIIMGNNNGQSTRIAATDYVEPFQDSGIMKYLWTPSSSSLNLTDWPTLAEMIIRNKRVVVMMDYGSDQSQVPWLLSEFDYQWETPFSPTDPSFPCTEQRPTNQSEDVSRNRMYMMNHNLNIAISLSGLSDILIPAYSLLDEVNAVSGNGSLGLNVQNCEKMWGRPPNWLLVDYYNFGDFNGSVFQVAATANNVSYNRDSCCGTATTNAAAEIKRHYCLSVSVFLILFFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CNK3</accession>
    <name>A0A9W9CNK3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003522</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CNK3"/>
    <dbReference type="OrthoDB" id="3786670at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="546" mass="62139" checksum="C8425C3024CAE19B" modified="2023-11-08" version="1">MKPLTITRCAVANEILRRRKQMESDNTHRPQLHHEQRADLISISFEDIPAATTRREQREKPRAAFTEESDSSTADEEETVDLTRIHTSAPKRTDAPSGKELYWAKVRWVPNDLEANCEPKAQHRGSSTRGTELHARQWNSAIFTAVQRKVEARTRKALSVQSRWNVSPNDARQNRGPDARNGRRKPTVIPRRRPHQADEENTLNVAYCFPPDQSTIRNQKQYVARPESHLPHSCPDVSRDTTSPASDTTLYEAPLIYSLSQLKAGEDHQPYESFEETEKHIKLDQNLSTPVPRRHGRRQRRLSIESAFESPRTPRIPLRARAKTNDGMLQFMANASKEPTKSVPTRRHRHKISLNIPVVASVPPPEIFQNPDSSPTRIESPRRMRTRDVQAPSFLTLAERELEYKHRRTFVGTASLDDLLEILEVTLEHTTTKDAVAKAFIIMSSREQLYARQCSKKADGWELVLRVTPDVTNIDFVAQLQVKLGSITLRQFLDLIPFNEEEEVEALRVVEAFSAASHIDTNAGAGIGSKARAFRSWMVSQNDIGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CP91</accession>
    <name>A0A9W9CP91_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Glycoside hydrolase family 5 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">N0V83_002916</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4374175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CP91"/>
    <dbReference type="OrthoDB" id="412536at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000152">
        <property type="entry name" value="Extracellular endoglucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005102">
        <property type="entry name" value="Carbo-bd_X2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016282">
        <property type="entry name" value="Glyco_hydro_5_endoGlcnase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF41">
        <property type="entry name" value="ENDOGLUCANASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G01830)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03442">
        <property type="entry name" value="CBM_X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001043">
        <property type="entry name" value="Endoglucanase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="6 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040811496" description="Glycoside hydrolase family 5 protein" evidence="10">
        <location>
            <begin position="20"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="11">
        <location>
            <begin position="60"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding X2" evidence="12">
        <location>
            <begin position="375"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ4374175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="530" mass="57930" checksum="78EFB8C93622F3A7" modified="2023-11-08" version="1">MRVSHSSAALLLNSAFAVAQSTNGTGVTCPSTSKFTPISAGKFFDAINPGWNLGNTLDAVETEGSWNNPPVVASTFDDVKKAGFNGVRLPVTWAYHFTSQAPDWTVDEKWLQRVSDVVDMVTDRGLYAIVNVHHDSWTWADLTQASTNVTVVEERLAKLWFQIGTKLACKSEMVAFETINEIPGTTAEHGKVINRLNNIMLQAVNDAGGFNAQRVVTLVGAGEDGVKTTQWFERPDAKFKNPWGIQYHYYSPYDFIFQAWGKTTWGSDADKASLEADIAAVRGNFTDVPLIIGEWAASPVATETAARWRYFDFFLRTAAKYNTSTILWDNGADFLDRASHQWRDGVAIDIYRNAVQGVANALPDSTTDGSATSQQSSAYIYHKKGANVTDVALPFHFNGNTLKDISLTSSSTTANKSLTKDKDYTVSAETITFASPFLSTILTPPSKPQAPRKPNPHLLRRRPLTVTIIQYATPVLASTSSPLPANSSDLLIPITWAGQTRPAAVKAVKSDGGYLVDDWTQYLGPLQQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CPJ2</accession>
    <name>A0A9W9CPJ2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001994</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000003">
        <property type="protein sequence ID" value="KAJ4374916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CPJ2"/>
    <dbReference type="OrthoDB" id="3944493at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="76"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="430"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4374916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="486" mass="53344" checksum="CF140301F19349F6" modified="2023-11-08" version="1">MPPSFTFWKPSNADDQSRSPLLLNDDDAVVSKNLRSDAAAMPATQRTAVLDLDPGTEDSGVFFFDEADFQPPTKPAVPAASAAAVPLQSLPLPPPSLEEPQASSCPIPAVGGEKLAPATRRDRDSAVYLSEDEIPSSKGQTPSLAPSDPPTTCLRTPPFPPLKCTPSTHSSSSPLQRTDSHARRLRRPADLHLLTSKPATVDDTSKQPPPAPPRSELEQRYDLIRNSKSHSTKATNLRSPTELLRERLNLSPKKHKHEEKVRIFTPPSMGKKKSSANGGGGCLMPSPGGQSPEAFRSTSIRARTEAGGRPAWWCKFDKLVVFDGIDVDTNGKGDEPTFRTRTSKGLSIARRRGSQETIVIPMDCAHCQVMLNRVEWKYDVRVCKRGVCWECRARCRWEVEQERHQKKQEQEEEESTSGLDAVAAITRSQASRDRADSVLQDEQVHGGDEELLARKVGIEEQGRKGKGTIEVVGGIDERLESLRMGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CQX2</accession>
    <name>A0A9W9CQX2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Coatomer subunit zeta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">RET3</name>
        <name evidence="15" type="ORF">N0V83_001110</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. The zeta subunit may be involved in regulating the coat assembly and, hence, the rate of biosynthetic protein transport due to its association-dissociation properties with the coatomer complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Golgi apparatus membrane</location>
            <topology evidence="1 12">Peripheral membrane protein</topology>
            <orientation evidence="1 12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle</location>
            <location evidence="12">COPI-coated vesicle membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CQX2"/>
    <dbReference type="OrthoDB" id="10249988at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14829">
        <property type="entry name" value="Zeta-COP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000013">
        <property type="entry name" value="Coatomer subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039652">
        <property type="entry name" value="Coatomer_zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11043:SF0">
        <property type="entry name" value="COATOMER SUBUNIT ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11043">
        <property type="entry name" value="ZETA-COAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040906274" description="Coatomer subunit zeta" evidence="13">
        <location>
            <begin position="18"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="14">
        <location>
            <begin position="10"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4375833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="199" mass="22276" checksum="561A05780F8C4787" modified="2023-11-08" version="1">MSRPLSLFSIQAILILAIDDGSRILTKYYQNPHPPAGHQADYPGQIAYKTVKDQKAFEKGLLEKTAKQTTDIILYDQKVIVFKMESDVMLYVVGSAEENEVLLYSVVLALRDSLNILLKNSVDKRTLIENYDLVSLAVDELVDDGIILETDPVIVASRVSKPPAQDVTSMKNIDLSEEGINKLMSYAQKSFVERLRQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR03</accession>
    <name>A0A9W9CR03_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4375923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR03"/>
    <dbReference type="OrthoDB" id="3685541at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4375923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="421" mass="45267" checksum="28EE4171E5D255C5" modified="2023-11-08" version="1">MKNGDYYCKRRNFLSCPEEENIVGLSVGPVAVPTAPVDASDSLDDPLCDEGRLFCVVTNYVDKIYYCEFDRKGYTLIKACGANEYCVGRPEAPEMDPHCAKQTTTTHPTTSASPTSTAPFPYLPEQPTSRAATSSELAPRAEAAPPMCSGFEVFCASSPTGDLMAYCEPGFGPKVLHKCAAGEYCVGVADESKHTADLHCAKDAPTIPPSPSPHPPRYLHRAELSVDDDARVYCDGRYIMHAEAGGGPVEILHMCEVDNYCIGNDNPWEGRIQLSCRYEDPAVSTPGHSHPAPTPTPTGPVRVIGPQPSPAARAASEPIPSEIAAIIQNISNATVDTGETSAYMCGFCINLGSWRRQLSGLGVCNTLANDEEYRACSNQYCGTCIIFRQKNCQWLPGGAVHWGGPGEGSYDARGGHSHYCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W9CR95</accession>
    <name>A0A9W9CR95_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_001460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000002">
        <property type="protein sequence ID" value="KAJ4376179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W9CR95"/>
    <dbReference type="OrthoDB" id="3675680at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4376179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="365" mass="41000" checksum="BCD5D3F9DE4E4899" modified="2023-11-08" version="1">MSTILFNRTRRSPTEKKRVTMTMNEIEEKLASLDLRSCQDKFIIDLTSPPSSPSTEEFGYESESEDEEEATAELSIKMGQSLPSTSMVQMVSHLLTALKVTREQESIIAAECAIIEYLHVNAATLADVEALIPAMNSNTRIMGKLATNIGWHRSRGTISNAETEQILAHILTNGDLEGAMFERTKKCIKRNESPHGQRIAMMQADEYDNQAAWEAERRWKTMPRQLRVRQEHELLPAGCTCLADVYAYIYCMTWSGVLALNKIKRASTKKKLKVTVARNNPPKTVFDGTGSGSFSQTRLHNGPGLTDKRKPKVILPRAVERPILTTPEKLNTVKSATISPNQLWAKTMPSPGYYPRAGKRMIMWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XX65</accession>
    <name>A0A9W8XX65_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000022">
        <property type="protein sequence ID" value="KAJ4361969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XX65"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="224"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4361969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="36291" checksum="336A420B3A605087" modified="2023-11-08" version="1">MVNTPQSSPSGASDDPRSPPPPNPPNSPRSPTSPRSPTSPNANETEGHRRFGSLDGSMFPYHNPNVPYEAAFMNQGANDPLYRPRNEDPRYRHLYRGNYVWTNDGWTYAPYRTQTATPAPGSGNQEGPQGAASSRIVPVYMLEDTELNYDPLGQASQQGVAEDPNDATQGPAAASVYPAPPGDPVSAYQTAPGGGQERRGSVPAAAQPPQMRTPFTGYATRGGRQAPAQAPSAPRAAPSSAYADQYQLPPPTDLLRPREWSDAEILTLYKWKVVKKKGNQKIIEAVFPDETNLSLSAAWRRYKERGKELYEAQEDEEREGWRRRRRSGGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXE3</accession>
    <name>A0A9W8XXE3_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">N0V83_010375</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXE3"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006857">
        <property type="term" value="P:oligopeptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000651">
        <property type="entry name" value="Glutathione uptake transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01022">
        <property type="entry name" value="PTR2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01023">
        <property type="entry name" value="PTR2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="396"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="464"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="491"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4362282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="547" mass="60296" checksum="000EDE1C9618283B" modified="2023-11-08" version="1">MARDIEIQNNTLSRQETSEDKIPVATSEWENLRRVPDKLPMVALLILVVELGERFTYFGLSGPIQNYINNPYAPGSDLPGALGKGQAVATALGNFFKFWAYASTIIGAIVADQYLGKFKAIIAASCIYIIGLIILVATATPSAIQNEAGFGGLIAAMITIGLGTGGIKANVTPMCAEQYQNARPVLKTLKSGERVVVDPELTVQRLFMWFYWIVNVGALSPLITVNVEAKHSFWLAYLIPLIAIIVSLGVFLSGHKRYVKVPPQSSAIVDAIKTTTIACREKSFNNARPSALAEAGKADRYKFSRDARYTDAYVDDVKRGVKSCKIFLFFPFYFVCWIQIWNNLISQAGEMALHGTPNDLLQNLDPIALIIFIPFLDLVVYPGLRKWKIDFDPISRIFAGFLLASISMVYASVLQYYIYRSAPNSIHVWIQAPAYIFVAFSEAFIIITGLELAFTQAPANLRSFISALFWLTIGIAAAICIALSPVSQDPYLVWMYGSLAIVGFVAGCIFFLCFRKGRKWSANTMVLEAVHLDTNTYAPAMAVDKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXX1</accession>
    <name>A0A9W8XXX1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010588</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXX1"/>
    <dbReference type="OrthoDB" id="3929397at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="548" mass="60859" checksum="7222B9197C597608" modified="2023-11-08" version="1">MSKRDEKFNILLGTNEITVKVSNLETAHAVAKEILETPGIAEKIKKIYIVTNDDDEEEDKDGQGAEPGVKADNTTTDEKANQTASTQASSKENPKAFLTTEQRLIARTQACEQGARLADRLCEPLCQIFNSSVNLKRFSWSASYCAGTSFTRPQTFWDALYAHSQTLEGLHLDYFEHEVRQLTDPKTIFPNLKTLKLNTVSAHGDDGTAIDALLNSCPNLSTLDFTWPGCDLDDCQIQNITWSYTYPSLTNLSLSGWNFAPKPLADFLMRHPSITTFADGIDFQLNDPGSEPIQFDANALPALEALYKSNGPLSSVLPYFDPAARRQIQHLALYVGMGGGSVLSELATLPAATSTIKTLEMHGNITDWRLPEDEDSSDSDSDVATDEAKEPYVPPPSMPSLLTSTLPHFTHLQELAIGMESDHIMHRRKDGVFGCPDPTTPEDLRYVLGHLPSTTTIRALRVRDERAKTLPKEMLDEFAGVPESLEYFAWEGEERVLYRVLRGDGGVKCVVCDDNEEDILLGRKMGGGWVVTEDSSGRRWSERRVLDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XXZ2</accession>
    <name>A0A9W8XXZ2_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydantoin racemase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_010661</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HyuE racemase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000021">
        <property type="protein sequence ID" value="KAJ4362567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XXZ2"/>
    <dbReference type="OrthoDB" id="412018at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047661">
        <property type="term" value="F:amino-acid racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015942">
        <property type="entry name" value="Asp/Glu/hydantoin_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052186">
        <property type="entry name" value="Hydantoin_racemase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053714">
        <property type="entry name" value="Iso_Racemase_Enz_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28047">
        <property type="entry name" value="PROTEIN DCG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28047:SF5">
        <property type="entry name" value="PROTEIN DCG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01177">
        <property type="entry name" value="Asp_Glu_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4362567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="29645" checksum="64D8C2AE6BC550DF" modified="2023-11-08" version="1">MGEYRKGRNPRYSKMRSILIINPNSTESMTNGLKPLVDALQFKDVNQHHSLAIPKTNDSEQTAHEYFTAPSGPKSINNEDDAADSVEHCLPALRQVLDRHDGFLVACYSQHPLVPLLKEESIIKAGRKPVTGIFEASVGTSLQSIHPDEKFGIVSTGKVWETILTDATIAFLGTGSEASKRFAGVETTGLNATDLHDAPAEEVRKRMKDAVKRLLKKGSVGAVCLGCAGMAGMDQMVREACVEELGEVEGKRVRVVDGVMAGVAWLEGAVRAGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8XYU1</accession>
    <name>A0A9W8XYU1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009686</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000019">
        <property type="protein sequence ID" value="KAJ4363393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8XYU1"/>
    <dbReference type="OrthoDB" id="3912861at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.70">
        <property type="entry name" value="Fucose-specific lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4363393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="176" mass="19922" checksum="DAFA6A58596B480C" modified="2023-11-08" version="1" fragment="single">MHRRKLAADLRFADSVVRNSASFELFCEGAVYLVDKNNWLRSYANDLPAAQLDSEVVASKTVRRKDYQSKSVDVCGKIDGIQSNTRDIAALLFEITNLKDTIRFRLYYIDTDGVLAELRQNNPKSTPLTLIVGWSPGTTLKAKGYRVVEHSRITAHMSPATNTIKVYYYPKEFPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0F7</accession>
    <name>A0A9W8Y0F7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">M6 metalloprotease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_009088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0F7"/>
    <dbReference type="OrthoDB" id="3852498at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008757">
        <property type="entry name" value="Peptidase_M6-like_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03296">
        <property type="entry name" value="M6dom_TIGR03296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4364494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="690" mass="74355" checksum="EC1B63908722C74C" modified="2023-11-08" version="1">MPIQRPSAALMGAFAGTMFNMASAIPAIAPRQTLSDPFAPIDPQQWVNPDNMTWDDFKAPPGTSWSDTNNKGRDRNFNIAMVTVDYPDSPFVVTLEANSDIFSNPQPAAAGLKREDVPKFYHDLLNTPNDLNEGHTLHEYWMGDSFGKYGVDLTVFGPYRMPAKSYQYGIDDEQGGFNEGDCPEGPPCSVDLRTDALGAWRAEVGNETANQYELAFILSAGQDESSTWQEFGEMRFLTKEDVPDEFGPPGNSSLPNYASTRYVEWTSWAAASTIWPNAGGGSSTQGESSGMATFAHELSHLLGIGDNYNNPYSVPPRRAYTGPFSMLDRGSFNGPGGPHTRWIIPPVQGASMGSLHTMRDKHQIGLIDNTPILQISRSGLATSGPVIAQITARSVDPGNGLMGFNISFGTQGDLAPPCNISFDAFCDGRGYNNYNLEVIDRMGADSFCPDSGVMISKTKNADRAQPFQWVIDANPQDAHVVDFYLPNGTARYWSIGDYRQLVDALFHAGTGSGSEYEYEDGENGLHFYVLERIRDDAGVLRYTVGVKSTSTNTTSTATYGIELGSGKLETNTSGYGASAADGAYCSFELKNTGTAASFNSSSNPHPQDLSAYLGSDIYRLAAEVDGQGWKVAVPNALAYAKAGKQKTVKVAVGCSGSDGGGCAKEATVTLTVTSEGDKKVKKSKTCKVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0P3</accession>
    <name>A0A9W8Y0P3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_009497</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000018">
        <property type="protein sequence ID" value="KAJ4364042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0P3"/>
    <dbReference type="OrthoDB" id="3801572at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="76"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="499"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="581"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="753"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="786"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="911"/>
            <end position="943"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4364042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1048" mass="115722" checksum="E5FDAC88BE89E7FC" modified="2023-11-08" version="1">MFCLDYSHEITEPDSLSNSRSSRAPVNGYGALQISEGLEAYGTQSSASGTATVVEVFVAALHKSNLPGPVILIDETAERIMVGRGSQFVIYRQRMAVPELTQFSTRTVAVKVPRFSLNPKAPLRLAGTAAQKHIHDMYLEVLALTNPIIRSHPYVARLLAWSYDAYTMNTPLYLVMELAECSLRSFLQNPVEGTVSHSLRYDLCRNVAAGVDVLHECGLIHGDLKTDNILIFRNRKGAYVAKVADFGLSVAEAVSGVGPVSNVYIGGTLGWQAPEVEQARTRDLDRMSLYSDGSNMTDFVDCSFNKVHHDGPYYSWETPTMAQVFIRDLYARMMEVPESTAPEMLFSMFLVVTAWPKLLTSSWNQGLDILLLAANRGSTAARGIVDSVVTYYGLTLQEEVEAHLFDWRRTAALSGSILAQRALACHDPSLLQDCVKHFRDRGGYATLYGTFDSMRQTGNTSSGTSYSRLHELATYGTPVALEAHLRDDDASSLDALTDEGETSVYLACARGSWEILEIFLGHGADCRIPCTQWDITCLHWAFAFDEHRQADAVSCLIAAGVDVNALVRDATPFPHYPFILPPGTALHWAVVTRSHTVIESLLQNGVDVSLRDGCDMYVYDDRIRILDKFGGPNMEAYSIPKGRVKGLSALDYAAMAQDPFIFDVIGRMRLPIDINNVDEEGLSVLHRLSTDPKRRTRTGNAFCDKVFRGAPPDTNKDLRHIIKVIVDIGGDLELLTTPSAMVSEHNDSELTLPCYTPLMMAALGTSVELVEALLQAGARVDAVNDKGQTALMCISEDREAAATIIPVLIAHGANVNHVDKSDATPILLASGPRSIDTVDLLLCKGADIDSRVPYREDEHGQGRGIFGLLPRQDDPFNDEADLTIRGMLEKYVFACPDIAKKRRVIDKGDIDGRTLLHQFARSGMPRCVQALLSNNAPLNALDYIYRRDHDQGKDVKLSWFETPLDSAFVARERKIQEMQRESVHTLEENEDILTKIDRVIFILQEAGGICTRHQVKSEPFLFDQSKFGKRGFVKALRESGSGGTVTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y0U0</accession>
    <name>A0A9W8Y0U0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">N0V83_008646</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y0U0"/>
    <dbReference type="OrthoDB" id="6339427at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF26">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="343"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="412"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="1"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4365030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="501" mass="55417" checksum="932D35C055CC9A80" modified="2023-11-08" version="1">MPSFYGYDQGVFGNVIISEDFLHTFDYPSANMQGVMTSIYNIGCFIGAMSTIWTGDILGRPRQIILGSTVIAIGAIIQTCSFQVASMMVGRIVAGVGTGMNTATAGVWQAETSKMNSRGKLVIIQMANCITGFSISNWLTLGFSFAPRDIAWRFPLAFQIFFTLCIYVMCPWLPDSPRLLIRKGKHDEALEVLAALEGHGATPESPTVITQYNIIKDILDREHMNTYTWMQLLSGKGPSGVLRRMILGAWMQAMNQISGINVTSYYMSYIFINALGISTLLSRILAAAGSVDYLIFACLAYFVIERFGRRKVMMTSAAACSICWIVIAASQGQTEAGGNSYKLGIVAVSFFFLFFASFGMGVLGVPWLYPTEINALEMRTKGASLAMSTNWICNYAVVQATLPGIENLGYKFWIIWAVICFAFIPITYFFYPETANRTLEDIDRFFETEPGVFIHRNKIAVQLQRPAEYIEADARIAAGEDIKFGAGKGFSTEHVETKETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y170</accession>
    <name>A0A9W8Y170_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009086</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y170"/>
    <dbReference type="OrthoDB" id="3801083at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="352" mass="38732" checksum="FB5EF93E53D12654" modified="2023-11-08" version="1">MTGFVYPSLVVVTETDYTTVIPTSIAAIRPSATRSTLSSHMASTTTSSPSFSTTPVSTSLLPPSLLPTPPSSDNSPSHLPSDFTAPTTPKHKFNDTFAIMTLTAVVIMGLLLAALIGYTLYLRCKGECPKCRDFKEQVGKWERGELKRITKDMVVRRSKDADPNSEFFDVDLEKGEDDVVDVEAARAAALDKLEGRAPKVPMWNGMMDALQTAKEKVTGKGKKELPPLPHQTQEMSKKELPPLPQQTLEPSANGDRFFTVDVDSPAPKTVQQQPTLWPPRPYYEPAANRSLYAPPSPLTEYSQPTEGNVRRTFGEYASPGNVNDAGSGDQTDMHGSYKMPAWRQVRRQDGIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1N8</accession>
    <name>A0A9W8Y1N8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Quinate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">N0V83_008747</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1N8"/>
    <dbReference type="OrthoDB" id="508119at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000026">
        <property type="entry name" value="MFS quinate transporter QutD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF34">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0672">Quinate metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="351"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="458"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="24"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ4365129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="533" mass="59375" checksum="BB455E5F8243EDCF" modified="2023-11-08" version="1">MGLLTLVEDRPTPKAVYNWRVYTAAAVASWASCMIGYDSAFIGTTLALPSFVKEFGFDKMNATDLALHKANIVSVYQAGAFFGSFGAYASSHFIGRRKSLLVWVLVFIVGAGMMLGANGQRGLGLIIGGRVLAGLGVGACSNMVPIYISELSPPAVRGRLVGIYEMGWQIGGLVGFWINYGLQEGMKPSHKQWLIPFAVQLIPAGCLLIGGVWLKESPRWLFANGKRDEAIKNLCWIRKLDVTDPYIMEEVAAIDAQIEHDRIHIGPGFWSPFVSLKQPKVLYRFFLGGMLFLWQNGSGINAINYYSPTVFKSIGITGTSTGFLTTGLFGVVKTVVTFIWLFWLIDRLGRTKLLMIGATGGSFCMWFIGAYIKLSNPASKASKPDAKLTSGGIAAIFFFYLWTAFYTPSWNGTPWVINSEMFSQQTRSLGQASAAANNWFWNFIISRFTPQMFLKMGYGVYFFFASLMILSVPFVYFLIPETKGIPLERMDELFEIKPTVKAHPIMVERLKEQEDQVRDVFVEKSKHDQIENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1R3</accession>
    <name>A0A9W8Y1R3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_008602</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4364986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1R3"/>
    <dbReference type="OrthoDB" id="3447202at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11616">
        <property type="entry name" value="SAF_DH_OX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048423">
        <property type="entry name" value="DRL_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013974">
        <property type="entry name" value="SAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37850:SF3">
        <property type="entry name" value="BLR7815 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37850">
        <property type="entry name" value="STRU PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21135">
        <property type="entry name" value="DRL_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08666">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00858">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAF" evidence="1">
        <location>
            <begin position="365"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4364986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="468" mass="50358" checksum="2611AFBBD36D2558" modified="2023-11-08" version="1">MTSLSTKLAALEEAGKPIQVGIIGAGKFGSMYISQSHRTKGVRLAAIADLAPERALNALKKTGFPQEKYDTTGSLSLEEGLKQGKTAITTDSAAMIATPGIDVILEVTGNPAAGVRHALLCCEHKKHIVMINVEADVLAGPLLTRKAKEAGIIYSMAYGDQPALIAELVDWARTAGFDVVCAGKGTKHLPQYHYSTPDTVWDHYGFTKEQLASGDFNPQMFNSFLDGTKSALEMAAVANGCDLSPPSDGLAFPPCGTHDLPEVLKPARDGGQLQKKGTVEVVSCVEKDGRWVHNDLRWGVYVIIEAPGPYQKECFKQYGLKTDSTGKYAAQYKPYHLIGLELGISVASIMVRGEPTGQTKTWCGDVVATAKRDLKAGEKLDGEGGFMVYGKLMTAGDSLEIEGLPIGLAHGIVLKSDVKKDQGLSWKDVEYSEESQAVAFRREMEAVYREEFRAAGRAAEMQGVNGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1V2</accession>
    <name>A0A9W8Y1V2_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methionine permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MUP1</name>
        <name evidence="6" type="ORF">N0V83_008650</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1V2"/>
    <dbReference type="OrthoDB" id="5982228at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF498">
        <property type="entry name" value="HIGH-AFFINITY METHIONINE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="430"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4365034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="470" mass="51151" checksum="47C7F6C101F775D7" modified="2023-11-08" version="1">MSKFFSKHGATLATTANDKETDGPVTDLDEGSLYLVRQAKRQIGVTSAVFLIFNSMIGTGIFATPSAIFSLSGSIGLCLFIWVAGMLIAAAGMTVYSDLAPPSLVTAVRRITSNTSTASPDSWPRDSILDTSSYLDGQGPTPWSLVNISFMLREVNRWNQRGVGLACITTAFLIHGLALKLGLRLQNLLGIIKLLIILLIVVSGWVALGGGLKIDKPHNFDHAFEGTTGSAYGVVTALYNVIWNYIGYSNANYALSETKNPVRTLKLAAPLALGSIAILYMFVNIAYFAAVPAEEIISSKRLVAASLFRNVFGTKAERALSVFVALSAFGNVLSVIFSQGRLVQELGREGILPFSRIWASNRPFNAPLTGLFEHWLVSVIIMLAPPPGYAYNFVLNVISYPLAVVNVFVAGALIHLYLNRSKWNWHHPISASMPVAIFFLFSNIYIVVRLSYHPQTDKAFTSHCLTTFTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y1Z0</accession>
    <name>A0A9W8Y1Z0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_008361</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000015">
        <property type="protein sequence ID" value="KAJ4365741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y1Z0"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4365741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="352" mass="38166" checksum="4AADE63596E1E845" modified="2023-11-08" version="1">MPGELVLITGVNGYIGAVVAKHFLDHGYSVRGTVRKVASAKELMDSPLKEFVDVGKFGVVEVPDITGDGAFDEAVKGVTAILHLASPVSMDFKDPVPIIYAAINGTKTILNSALACAGSQLKTVIVMSSIAAVKSSHPPPYSFTEEDWNDFAEDMCREQKTETPGPAIYIASKTAGERVFWEFQSERQPPFMMASVNPVFVIGPPLVAPKTAYAVGGTIQSIYQVFAGASNILPSSTVPQAVDVRDVAQLLRYAVEHPHETNGERYIASSSINHPQAVADILREEWKSDVKALERIAVGKPGEGYSMDYQSVDEQGGYYIDSSKARKLLEGGQWIRYKQSVIDTAMMFIGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y251</accession>
    <name>A0A9W8Y251_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_008802</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y251"/>
    <dbReference type="OrthoDB" id="2851338at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4365184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="234" mass="26663" checksum="9F2D65B611449EE7" modified="2023-11-08" version="1">MSEPQPWSGPGVIFTFFKLPESSKVSQHTIERWFDEEYVPKLLETEIVKLGWLYKAANPQYDKQHLLIYKVSDFAQAGKLKEVPRISEKGLFGGDVHDHIEFESRIYSFVQLYETSKQDEDSAPTILLAMMQPQPGGEADLDSWYRDEHNQQMSEQPGWKRTTRFSLLFQHGSVGRTNEELSFLAIHEFGEGNKLGIDVEALEPISDWTKKVMGEAKAIDAGIYYKTKALGKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2A4</accession>
    <name>A0A9W8Y2A4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tail specific protease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_008878</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2A4"/>
    <dbReference type="OrthoDB" id="27214at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056186">
        <property type="entry name" value="PDZ_CPAF-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052766">
        <property type="entry name" value="S41A_metabolite_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005151">
        <property type="entry name" value="Tail-specific_protease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37049">
        <property type="entry name" value="PEPTIDASE S41 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37049:SF4">
        <property type="entry name" value="RHODANESE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23658">
        <property type="entry name" value="PDZ_CPAF_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03572">
        <property type="entry name" value="Peptidase_S41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040793945" description="Tail specific protease domain-containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="domain" description="CPAF-like PDZ" evidence="4">
        <location>
            <begin position="138"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Tail specific protease" evidence="3">
        <location>
            <begin position="340"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="687"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4365259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="753" mass="81594" checksum="B1576F6BBFF1CA78" modified="2023-11-08" version="1">MLTRSLLLASAALVAAQSSTTNEPCAVVAARLTDSSALPAQEAFDCLDSVPVDTDGNSKLIDELKQVWQFQSEIGWLKNPGSDWEYGALDIEAELDKVKSNLGSYSSEYTAQLAIQNITIRTGNFHFNYAPDILQVFRFRRQFNVASISSDGKSLPKLYWHDDVEAVGAGSDDVSDIKEINGQNPYDFLKSTFFSQYIDSDGQMNNMFSKGDTDNPGAFAQQRKFDGNSTDVTWTNGSSLSVLNIATSQLDFSGVTDGASFFSQFCTGAITGASSSSANTKDLISPGAPGPVPRIPTGIYHRRNKRQTIPTTGTYASAVAEASSGVVAGYFLNGDGYDDVAVLKIISFSNPDPTSIDETEFNNEFQATIESFIKQSTSEKKSKLIIDLRENGGGNTNLLLDAFMQLFPDMEPFSGQRYRAQDAFIKIGNAVNEIYSDSNKASQYQQYVDQSISEGFFYRYWAWWHFRTAGGDNFSGWDQFNGPLTLNSDKFTATMRYNYSSADRVSILPDGFNFVNGTHTSPFDSSNVVMFTDALCGSSCASFHEELKNIAGVHAVTVGGRPTNAPIQTITGSKGGEVIPLIQFPTYAEVALNISSRVTLSSLKDSDSTLNALANVPQITTRVGDSSSRAQSQDQVRKGDKTATPLQYIYEASDCKIFYTADTYADPDAAWKQAWDAFQDDSKCVDGSTKHKSSISGGYTPYGAGELKKEDQPDEPALGNDEDSSTSGGVVVVSSKKVSSLAVAVAVVVAAMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2D5</accession>
    <name>A0A9W8Y2D5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Enoyl-CoA hydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_007760</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Mycotoxin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2D5"/>
    <dbReference type="OrthoDB" id="2018133at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051053">
        <property type="entry name" value="ECH/Chromodomain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43684">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43684:SF4">
        <property type="entry name" value="ENOYL-COA HYDRATASE_ISOMERASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_1G01890)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0843">Virulence</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4366125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="35861" checksum="8D609328F7F6A7EF" modified="2023-11-08" version="1">MSPRQKSPRHGPDVVLPNSYNDLKFTQIQTRHHDKEPSVIILTLHRPNNHNAFTDKMREEIEAAYGMFNVDDRVKCIVVTGSGRIFCAGADLDIGFGSGTAGEEAEAVQDHRDGDNDDRGGRVTLSIFHCRKPTIGALQGSAVGIGITMTLPMNIRIAYKSAKIGFVFSRRGLIMEACSSFFLPRLIGHSRAMAATTTGSTYLASDKIWGGLFTELCERPEDVLPKALEVAEDVVRNTSSVSTYLMKELMFRDKGSPEGQHLLDSRVIFELFGSPDNTEGVQSFLEKRPAKFTGTLEGANVTGYPWWMPLDILGRPKVAPTGKAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y2I1</accession>
    <name>A0A9W8Y2I1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_009328</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000017">
        <property type="protein sequence ID" value="KAJ4364731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y2I1"/>
    <dbReference type="OrthoDB" id="10644784at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4364731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="194" mass="20915" checksum="54F91A284277C14E" modified="2023-11-08" version="1">MAATGSGSSTTPTKNDDPTSRGDSRGFIISGIAIAVIALVAIAAILQNPRSKRRRACPVAQRVNEMPVHAHSPHFNADDASAPASSAVRPQYTMYSTYAEDHPIYGFGLMMMVPTEAHTHAPGRVSASNDDNEDPPGFGSVQHRGFHSGLQRLDASRRQEIAEARESVDTLPRYDNAPSYQAKDDPLESAQRMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y366</accession>
    <name>A0A9W8Y366_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Emp24p/erv25p- protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ERP1</name>
        <name evidence="10" type="ORF">N0V83_008907</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000016">
        <property type="protein sequence ID" value="KAJ4365288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y366"/>
    <dbReference type="OrthoDB" id="3427at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040733583" evidence="8">
        <location>
            <begin position="19"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="9">
        <location>
            <begin position="28"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4365288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="220" mass="24845" checksum="4B61729C77E262FA" modified="2023-11-08" version="1">MWKPTVLLAAALAVPSHALHFFMDGAVQKCFYEELPKDTLVVGHYHAETWQDATKSFQTQPDVGVFVTVEETFDNNHRIVAQRGSSEGRFTFSAADSGQHRICITPQNVPSGGGWLGTGVHGTVKFTLDLAIGETSRIESTDKDKVETLVQKVTDLNSRLQDIRREQVFQREREAEFRDQSESTNARVVRWTLIQLVILGVTCVWQLSHLRAFFIKQKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y3J7</accession>
    <name>A0A9W8Y3J7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Macro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007604</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058348">
        <property type="entry name" value="DUF8035"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056223">
        <property type="entry name" value="PH_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058925">
        <property type="entry name" value="zf-C2H2_AcuF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106">
        <property type="entry name" value="GANGLIOSIDE INDUCED DIFFERENTIATION ASSOCIATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106:SF27">
        <property type="entry name" value="MACRO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26118">
        <property type="entry name" value="DUF8035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24345">
        <property type="entry name" value="PH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26082">
        <property type="entry name" value="zf-C2H2_AcuF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="2">
        <location>
            <begin position="754"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="domain" description="Macro" evidence="2">
        <location>
            <begin position="1171"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="497"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="583"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1361"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1712"/>
            <end position="1802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1978"/>
            <end position="2000"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2076"/>
            <end position="2142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="514"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1361"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1988"/>
            <end position="1998"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="2165" mass="242591" checksum="2A3205EE481FA008" modified="2023-11-08" version="1">MASLRLAAASNVRAFQILSNALSTSDNKWAHSIDREALEDEIGRYRVWCGNLGALLKGHASLDYRLRDSPLLSSNALKFLGELEENLNEAFAIVSGARLPYEEQPKPEKIDDDEDDTSFFSEDEDDEEDESSGPRFELSMRFSEVVDIIDNLYKLSVRIRTPTIRSRSLKAASYKPKDPETGVDILSTYAEYDLQHIKELLSHVREPHPASEETEDDFLVTRLSAALTLRRRQFKYWRRHREKLGASTMLEAATKPVQPMLSRPEELQQYDNIEVEAEVPINVTMVEASSQKTGKTLLSATEATQHHQSLDEIVDAKSVTSYAVTVKDIHGKGIDLPPPPRAANGEKDFECPYCYIICPARYGRGRAWRTHLLQDLQPYICTYPECESSEQLFRSRREWAEHEAGHRKVWRCPEHPAAVYKTSAGLEEHLRQRHMDDSFPESQVATIVKVGETSTVDVRQKCPICHVPADAEGLDNLQNHIANHLERIATFALPNAADDESDGASSAASGGWTGSQDVSEMSLPSDTTDGEEEKKEERNVLQDIRASGLAPNVDPTRAFLSAESLQQVPDASQNRFDLTARHYNEPGNLKEPPTEDEDSDTRQFNQEQVDETPAEVQEHLEQREEFRTYLMSLPGAELVRFYKRYGAWTGQATFKDEAAAMRAVVVFDANRFASVRIYQSSGKKNKVKFAASNLPKVEGKPVHMLENVDAHSASSASITYDREEETSRKRPVLTVSEIPTLRSLYRTRRLMQRDQSYAPNDYYNRTISFCYYDLTRLKVDAIVNSANSGMKITNSPETLNHAVHKAGGPGLTKEARSKGKVKAGQVELTHGHDLPSAWVIHASRPRFSGSKGMGHFNVLTECYRSALRMALNYEFKQIAFPCLGTGGCGFTPRVAARIALQEVREYLDAHANNFFERIVFCVNSALDEKAYMDFFPVFFPPTHGDLEVARSAGWSANRAALATQVLETRTQVQKVFEELSTDFGLVIPNFNTDILSELSAIDSAMASIRDFLLGTKELKRSLGDLNLICSVMQTVCGSVTETTELARDTAGVAPKHKVIWNDYNEHFKSTHGADLAHFLVDCQNFVQCLADIITRNGIELDEMSGMRQRLESFKAKQVGQDTEGIRDHLDEVLYAREFQREMVSHTREIVRHHQIPSVTRLYQLGELEAQPTLAKPSATFNHIIGLVREDITKLGVDMIINSTDVAFHGMGTLDRTVFKQGGIELQEHVKKLGECKEGDVKATPGYLLPAKHILHVVPPEQYRKNTKAVLRQIYRELLHTATVMRVTSIAIPSIGTGMLNYPRRDCASLAMEEVKRFLETADPSSLLERIIFVVYSSNDEFIYKSLLPVYFPPIEHSTQVRPAVQTKQSSSPEDSTPSRRRSLFGSVSEAFRSVRFGKQPESSREINTYEESALISFESHAKDCVTCKDIDKLYRDGDHLCEKGYALAQQLLWYMNMTSDHNVYPRPDKTGQSIKLEVPADMFPISLSLLTTVERSLWDDSRNEPFVSTNDLRSRVHDRDGPPLFITHGVEATKPISISSSSGQKIPRAGVATWSNLAKKWDPIFPGYCSIHVYPGRVEIRESENPTANQVPLLALQLTHSTTVVRDTANTGVTLNSAPRLKSVLTTRGDVLLHSQNATESTVLFDLLKRGIRDAPQGPAEPTHIKHEHELDISADVPGALGRVPLDESSESTGSYPEWNQRLQGIQKELAPATEQGGGRSSYLRSKLQSLGERVDRLSSSSSRRPAPEHPVQVNYNPFARTRSRGSQGDIENPLHRTTSTRSENHGTPPIQELPGDGNGSSLAEQVLAHLTIDLRSRPGSYIGQNAEDIASALQRDLLEVLAAITELAAQGKVHNTVDSSTWVISRPPPELPALLQQPEQPETRQPFATDDPQQSLSTLAKQILSYMEYVNHAPGNTSGQTIRELASALQLPTSEIWPAIRELEAKGHIRRSLNEETWIINTPEANTAVAEVRDAPIFKDTPPSPTSPSASSSFQPTAGSSSSYIDMDLDLSTINLDDYFTYPTPSGERWTRIDKRLVDAQVLIDAEEDFEDTGDSLIIHRVLRRGEIKWWAEKSKDRRNRQEATGEAARRDLRGSESSGRKEVGRERLTKRGSREIRGDKEGWRDGRGEKDRRQAKLDRVLAGDMKEEELRHFAEIEDDRRKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4L9</accession>
    <name>A0A9W8Y4L9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidyl-tRNA hydrolase</fullName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_007560</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the PTH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4367030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4L9"/>
    <dbReference type="OrthoDB" id="1711136at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1470">
        <property type="entry name" value="Peptidyl-tRNA hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001328">
        <property type="entry name" value="Pept_tRNA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018171">
        <property type="entry name" value="Pept_tRNA_hydro_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036416">
        <property type="entry name" value="Pept_tRNA_hydro_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00447">
        <property type="entry name" value="pth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17224">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17224:SF1">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01195">
        <property type="entry name" value="Pept_tRNA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53178">
        <property type="entry name" value="Peptidyl-tRNA hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01196">
        <property type="entry name" value="PEPT_TRNA_HYDROL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4367030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="330" mass="35596" checksum="79F0270DF243DE7A" modified="2023-11-08" version="1">MSNASARAHVASLTAAPLIPVANPALENHQTSTLQETDSDEAIKPTTTQSRKEKRREKKQKHDSALATPSDTDNEASSPPHKPPNKPPKKKRKPTTDTTIASLLPLMPPAKDAVQKVYPLLICSIGNPGSTYANTLHSAGHVITSYISERKGYKPFTKGLSGLVSRPDDTKMSFSIIRGYSRVQDPSALPPSDYWTFWQSTTLMNMSGASVKKAYNEWLRTVKAQSPFGSAAQGRLVVVHDELESALGKVTVRDGGASAKGHNGLKSCQQQLGGIKWWRVGVGIGRPESREPSVVSRYVLGKMSGAQRAALEKSAVGVYAALEEIAEGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4P4</accession>
    <name>A0A9W8Y4P4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Chorismate mutase</fullName>
            <ecNumber evidence="4 13">5.4.99.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ARO7</name>
        <name evidence="15" type="ORF">N0V83_006354</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">IMI 356814</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>chorismate = prephenate</text>
            <dbReference type="Rhea" id="RHEA:13897"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="EC" id="5.4.99.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:13898"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.5" evidence="4 13"/>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4367999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4P4"/>
    <dbReference type="OrthoDB" id="191918at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.590.10:FF:000002">
        <property type="entry name" value="Chorismate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.590.10">
        <property type="entry name" value="Chorismate mutase, AroQ class superfamily, eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008238">
        <property type="entry name" value="Chorismate_mutase_AroQ_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037039">
        <property type="entry name" value="CM_AroQ_sf_eucaryotic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01802">
        <property type="entry name" value="CM_pl-yst"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21145">
        <property type="entry name" value="CHORISMATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21145:SF12">
        <property type="entry name" value="CHORISMATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017318">
        <property type="entry name" value="Chor_mut_AroQ_eu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="Chorismate mutase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51169">
        <property type="entry name" value="CHORISMATE_MUT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 13" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13 15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0827">Tyrosine biosynthesis</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="14">
        <location>
            <begin position="147"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR017318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ4367999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="267" mass="30489" checksum="1F9E91D35E378A31" modified="2023-11-08" version="1">MADAAIDLSDASKALDLANIRSQLIRLEDTITFHLIERVQFPLNKTIYVPGGVDTGNPALSLFDWMLREQERLHSRVRRYQSPDEYPFFPDALQEPILQRLEYPQILWHNDVNVNAQLKDCYVNHIMPAACTQFGREDRGESQENYGSAATCDVMTIQALSRRIHFGKFVAEAKFQKEPERFAQLIKAEDRQGIDEAITNAAVEKKVLERLRLKAKTYGTDPDLGAEGSSKVNADAVVAMYKDWVIPLTKVVEVEYLMQRLKGTQWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y4R8</accession>
    <name>A0A9W8Y4R8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_007477</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y4R8"/>
    <dbReference type="OrthoDB" id="3684889at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4366947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="252" mass="29584" checksum="8196B07A77BB433E" modified="2023-11-08" version="1">MGTLAEYEEKMRAIEAERQKQRKIARRKEAIIDRIKAQHLSSAYEASRQQCLAFCADLHAALPRELRDMIYDQLIPPGLRHHVFEALDEHTGQPRTSSNPLVHVSFFDPRSRMPAQVSVRRNVWRYREYVGEVVAKEIAERWYHTGLFILDAEDLMVGKFLGTDVWERDVEPAGAVRHVRIQINGYRFDDGWDGRKMVEGMLKSFEELSRITNKKTEIVFFLSRSEALWHTRRILELLAPSVYRLRWEGFRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y524</accession>
    <name>A0A9W8Y524_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">BZIP transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">N0V83_007854</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Positive regulator of amino acid starvation-induced autophagy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
            <location evidence="4">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SCOC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y524"/>
    <dbReference type="OrthoDB" id="2163284at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019357">
        <property type="entry name" value="SCOC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21614:SF0">
        <property type="entry name" value="GEO08385P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21614">
        <property type="entry name" value="SHORT COILED COIL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10224">
        <property type="entry name" value="DUF2205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ4366218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="176" mass="19372" checksum="7D3D28EDE7C8CBF0" modified="2023-11-08" version="1">MSEQPQVIITPYPTAAEHVDRRSSYPDDMSTTSSSSSISDQDQLKGDPTLQARPRLGSRKSSGTIIIPRDSPNVELKEEEYDDGDARTMSPRRSSEEIEKMGQDARQALIEQAKALQASLMEIVDRVEVVKNEHEKLEGGNKFLQSYIGELMQTSKLTSTGAGKGSKTKGKGRSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y563</accession>
    <name>A0A9W8Y563_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pyridoxamine 5'-phosphate oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_006610</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y563"/>
    <dbReference type="OrthoDB" id="414774at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45588">
        <property type="entry name" value="TPR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45588:SF1">
        <property type="entry name" value="WW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="PNPOx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase N-terminal" evidence="3">
        <location>
            <begin position="52"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="157"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4368254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="688" mass="77138" checksum="56D3F1241D8556E3" modified="2023-11-08" version="1">MLHTANQTEKKIFAPTSNSDDPGHATQYTKGALDRADLLPSPTDQFNKWFLEAQNAKVYQPETVTFSTASLPSGKVSARVVYMKELDSTGFVIYSNWATSRKAADVRSNPYAALTFWWRELERQVRVEGPVERLTDEESQVYFDTRIRGSRIGAPKAQIWFDRGLTWCYAFHHEESARCFERAINEDPNCAMAYWGLAFALGPNYNKPWDLFDEEELTSTLDKINEANSKAKTKASASTELERALINAIQVRVPKGLDEDAFLACNKEYAEAMEGVYERFEDDLDIASLYADSIINLSAWNLWDLKTGEPTEGAHSLEAKAILDKALGQDGVYEHPGVLHLYIHLMEMSKTPEVALIAADHLRKLNPDAGHLVHMPSHLDILIGDYRRAIDSNADACLADEKYLAEYGGDNFYSFYRMHNYHSLVYAAMFAGQSRVALDAVSRMEASLPESLLRIESPPMADWLENFASVRIHVLVRFGRWKDLIALDMPKDQDLHCVTTAMMHYGKGVAYAASGDIEKAQKERDALKGAVERIPPSRICGDFPNRSNVVLQVGVAMLDGEVEYRKGNYDLAFKHLETAIELDDALTYAEPWPWMQPTRHAYAALLMEQGRIADAAAAHKADLGFDDTLPRARQHPNNIWALHGYHECLVKLGKEDEAAIVGQQLRIAQAVADVKVKASCYCRGAGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5A7</accession>
    <name>A0A9W8Y5A7_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteophosphoglycan 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_007502</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000013">
        <property type="protein sequence ID" value="KAJ4366972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5A7"/>
    <dbReference type="OrthoDB" id="2142961at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016071">
        <property type="term" value="P:mRNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028322">
        <property type="entry name" value="PNRC-like_rgn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15365">
        <property type="entry name" value="PNRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="412"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="524"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4366972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="565" mass="59972" checksum="606BB2609AD10534" modified="2023-11-08" version="1">MSTTQSTASPRPPRGNPQQPQGATQPLQNNTARQSQRKPRGNRAQNGHNQLNAAVASPNQRPANADPAFTDSAVFSSEEAQMPKGSRNPKKHTQSQPSLDRVCSPNGPQAPLTDTEAATNNPSATPAKVHGAYAGPTFHASPAPSALPIPKFLSRSVPAKTRSGPLTPPPEDSSDSAGSPSPSPASPSRAPIPVPSRHQDSPLDMLFKADKAERAKNPQGSPASAQYFQPSLPVRPQHYKQDSFNSSNGVFPIELDGASKDAHLSPPAASPAAHRSVTDPCHVPQVQNPPQANNGNDVMQDLFNRLSMSQKNLAAATPQKPEGQAPSDPQSRNLTPSPFHDGRPTTVRSASGPSTPAPTTQEASDFFYGNRNLSPLFKAAKGDTGKRNSGLRTEITADSPLVDQGLFHDFSAIPQPRVMDPNTFTRTQPGNHNERPVGARRGSAPFVQPYQQSPNNRRRTPGRQSYQPRPDSYPARVNQNGSGPKPGKAVGNIPQASAPKPSTTMMSFVPASVAAKPKQQATSTPPPAGTPPPMKVPDSSDTLALEQDLKRLLNLKMTGDTSSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5C6</accession>
    <name>A0A9W8Y5C6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">2EXR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006408</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5C6"/>
    <dbReference type="OrthoDB" id="3799620at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045518">
        <property type="entry name" value="2EXR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20150">
        <property type="entry name" value="2EXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2EXR" evidence="2">
        <location>
            <begin position="12"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="450"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="483" mass="54568" checksum="28A91988D7456A85" modified="2023-11-08" version="1">MPCAGHATSLLDLPRELRDRIWDFALVETTVHIEKDNHLITLAYPATTTTNDDSQHPNDQQATFESQNTPQWLLTNKQFLSEGLDQFSRRAACINFAQQALSPKQSKEESPPNTHQNLLITHTKQISLPVRLQVFRHEYNETIKDTVMDLHTSPFAVPHGHDRGGPDYRLDPGTKIADFLPKATNLHLRVEMIGGIERNARPKLIRINAGALRFLGTDFNRVRVSIAPPNFVLGRGRGSRSTGNEGTAATAAVVAEDYMFPPIAPLAAYYARMQRALVQLAGHLSRHLGPSQEYRESRYWWVSEHMMHPCAEYEVASGDDDVGDAEYEITDRMVERRDGEGGEWHLFVTRYSKEEAEEETIGEKEKEGEQEEEDVIHLHNLDLRYFRVTAAQAQDQDALNAHPHPPSSSHLFKRDAIAKLGTARSWSCASTGDVVWIEDRENAVTGWLLRRRDQDGNGDETKKEEEDERRLMVAEPNKPAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5X6</accession>
    <name>A0A9W8Y5X6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C4-dicarboxylate transporter/malic acid transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_006418</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5X6"/>
    <dbReference type="OrthoDB" id="2901184at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015140">
        <property type="term" value="F:malate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09317">
        <property type="entry name" value="TDT_Mae1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.150">
        <property type="entry name" value="Voltage-dependent anion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030185">
        <property type="entry name" value="Mae1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004695">
        <property type="entry name" value="SLAC1/Mae1/Ssu1/TehA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038665">
        <property type="entry name" value="Voltage-dep_anion_channel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31162:SF0">
        <property type="entry name" value="MALIC ACID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31162">
        <property type="entry name" value="MALIC ACID TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03595">
        <property type="entry name" value="SLAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4368063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="37436" checksum="88EC0C8A28465AB9" modified="2023-11-08" version="1">MSTGSLSVVLGKTPNTFRGLHTIGTIVFIFNLLLFLTFNALMATRFILIPRKLLASLHHPVEGLFYGTYWVSVALILNGTYIYGDAKTGAWLTKALEVCFWMYSGVAFAVGVLQYSMFFRLERLSVTDAVPAWIFPIYPLLVVGTLAGTILPGQGEEASWKIFVGGILLQGLAWVVSFLMYGIYMQRLMTSALPSASVRPGMYVSVGPAGYTAAGLISLANQAPTLIPPNYWTNISVPDGEIVRILGIVSGLFIILFAYWFFFITTIAVLAGLRQMSFSLTWWAFIFPNAGMTLATIQAAKVLHSEALNGVAKFTWRESTEFDKLRFDKKQRKIAWAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5Z5</accession>
    <name>A0A9W8Y5Z5_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Actin-crosslinking protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_008051</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FRG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000014">
        <property type="protein sequence ID" value="KAJ4366415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5Z5"/>
    <dbReference type="OrthoDB" id="5539371at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23339">
        <property type="entry name" value="beta-trefoil_FSCN_fungal_FRG1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008999">
        <property type="entry name" value="Actin-crosslinking"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010414">
        <property type="entry name" value="FRG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12928">
        <property type="entry name" value="FRG1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12928:SF0">
        <property type="entry name" value="FSHD REGION GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06229">
        <property type="entry name" value="FRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50405">
        <property type="entry name" value="Actin-crosslinking proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4366415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="29536" checksum="D734EF7EB7430BAE" modified="2023-11-08" version="1">MPKALHFKGDKKVKKRKKGAEPYDADDAPSKQLTTSASAEDAGDDDSWVSADAPSDISGPIIVVLPTDTPTCIACDANGKVFASALENIVDGDLTTAEPHDVRQVFVANRVAGTESLSLKGHHGRYLSCDKYGILSSNSTAISPEESFLVIPVPDNPSAFSLQTCRDKFFSIDESSTGGPEIRADAESISFNTTFRVRMQARYKPRLKANKEEKAREKISRKELEELIGRRLEDDEVRKLKKARREGNFHETALDMKVKSSHDKYASM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y5Z8</accession>
    <name>A0A9W8Y5Z8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PhoD-like phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_006755</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y5Z8"/>
    <dbReference type="OrthoDB" id="9999821at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07389">
        <property type="entry name" value="MPP_PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.70">
        <property type="entry name" value="PhoD-like phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018946">
        <property type="entry name" value="PhoD-like_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038607">
        <property type="entry name" value="PhoD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043904">
        <property type="entry name" value="PhoD_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46689">
        <property type="entry name" value="MEMBRANE PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46689:SF3">
        <property type="entry name" value="PHOD-LIKE PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19050">
        <property type="entry name" value="PhoD_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoD-like phosphatase" evidence="2">
        <location>
            <begin position="146"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="PhoD-like phosphatase" evidence="2">
        <location>
            <begin position="371"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="PhoD-like phosphatase" evidence="2">
        <location>
            <begin position="453"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="579"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="686"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="602"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="618"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="651"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="664"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4368398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="762" mass="85022" checksum="3B0FA534CC17A219" modified="2023-11-08" version="1">MQEAPIHQDPSGAVGVGPGPQVISTSGALDVIMGPLLNYRRTSNQQTGQPIWHGSVLIVTTPGQQSPQLVLSPAGAVDANAGNARFSVDSRDFRGEKLYEDPRKAFWRFTIDVPFQQFESRWKYDIQGLAYVDHNPPEDPQVFVVPSITQSMRIMFHSCNGFSVGTDVDAWSGPALWNDVLRMHEQRPFHVMIGGGDQIYNDGVRVDGPLKPWTAIHNPHKRREFPFDEKMRAECDEYYFKNYVRWYGTAPFSTANSQIPQVNIWDDHDIIDGFGSYTDHFMQCPVFRGIGGVAHKYYLLFQHHLAPPKSTFTTDAPKTTHVGTLGTTPADPVQLDNTFVMTDDVGDPSYLIGHKAGPYVEERSRSIYCQLGARIAFAGIDARTERTRHQINYPETYKMLFDRLDKEFSANKDLKHLVLLLGVPIAYPRLIWLENILQSPLIAPIKFLNKRFGWAGGFFNQFDGKVDLLDDLDDHYTARQHKDERRELVQSLQNFSKKHSARVTILGGDVHLAAIGRFYSNPKHQIPAEQDWRYIPNIVSSAITNKPPPQAVANLLARRNKIHHLDHDTDETLLEIFDRDPRSQSAANEGNPAETGLPSPPTNGTTANGNGTTTNGTKTDKKTSDKNHATMPSRNYAIICESSTTSSPRPLSLPPTPPPAKPLGPHHQHQQQRRRRLDPLLLASAQQKDNPRKAMHVGEQGAGTQHPASNGLVSTGLCGPFGLDVTLRVEISNQDREGRTDGYGFSIPALMAAGYSDQGSRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y6X0</accession>
    <name>A0A9W8Y6X0_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Luciferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_007060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y6X0"/>
    <dbReference type="OrthoDB" id="9987011at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048273">
        <property type="entry name" value="Luciferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040841">
        <property type="entry name" value="Luciferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38695">
        <property type="entry name" value="AMINO ACID PERMEASE_ SLC12A DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38695:SF1">
        <property type="entry name" value="AMINO ACID PERMEASE_ SLC12A DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17648">
        <property type="entry name" value="Luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Luciferase" evidence="2">
        <location>
            <begin position="180"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4367477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="306" mass="33953" checksum="FFDCA965A0F53E61" modified="2023-11-08" version="1">MVDLLQHTSGLKAPLQILHPFPTTMLTESHIHIFTTILTLLLPIVTAFYIHHDYQAFLSLGPGGTPATPLGYLKIKLLSLICLRDPLRPLPIPPHFRPQKGYFNDDGALPKRKGTKPIVQGIAPQRQQTQKSDCKVYARIVEKIKQVASDPRNQLLERTSCFEKHSSGLFTSIPITRTCGGEICHAHPSDGSMHMTLHPADAKLVLENGWGERHPLARGGWCRRFVPREFVLIYAPRDEDEVEIMMRIVAASVWWVSGVDVNGDENGPRRRSADVAAVSKAMEGNECWACKAHGCGSKVEKMTGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y734</accession>
    <name>A0A9W8Y734_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_006029</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4368947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2801544at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF51">
        <property type="entry name" value="SNF2-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="1030"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="34"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="954"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1219"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="980"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1244"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1317"/>
            <end position="1328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4368947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="1328" mass="148335" checksum="124B4E475197F226" modified="2023-11-08" version="1">MSGASLLETPLAWRTISEHNHQAAYDQAYTEDMKEMPGERDDRSTSSSPQSNIQPTTAVSTPLTSGSYPASVSDAKFQDIDRYVALGCLHIDSSIPTVSGSRYETNWIELLFPELPFEVISVIGNEAARLLDARWIRLFLHQPNFNVSYSRSIIRVYLLPEDWGRRFIDRNSKSLKQALRQLLQQIDISPNAWAGDFSEGEITFFDPWATAHKTSLYYLFNKLPSPAPTPETIKNRYAGAAVYDLLKSAASSEWEEYGEQPLAGLRTRLYPYQARSACLMIQREAAPQLQLDPRLEVRKSPNGETFYFGARDGSFLQEPRYYESNRGGILAETMGLGKTIICLAVILATKGHYPLIPAAYQPAPPVRERVGTLKDMAASIIGRYSIPTKAFLEQSEANGAGELTYVKNALDRNMPFYEIPPDLPRMNRNTRIPPPRQLVTCSGTIIVVPRNLLNQWQTEIRKHVLTGGLQILIVDSVPKRSSKAKAVPLASDTMEFTSELPAPTELMKYDVILFTRNRFEQEIQDGADDQGRRTAAGVTRTCNCPYIGATRIPDCNCVDSQRVYESPLKKLHWLRIIIDEGHSFSSSNSNAVLVAKQIQAERRWVVSGTPAKNLVGVEVDMSTLSADQDNPASIRESTIEQRKVFSHGDENTKAAKALGLLASNFLMVQPWGGPSIEEKLDWDEYIYRHEHQYRKTYSGFSSCFLRTLEGLVVKTRPEDVDKDIVLPPMRHRVVYLKPCWFDKMTANLFVQVLRANAITSERSDVDYLFHKNSVKARHSLIRNLRQSNFTWTGFRLADAISTVETSTKYLAKDDKKCSHEDAQLLMESSQVISKLAASNGWIALSQAHEVGMAVEDWPAASEDSFALAYPVKPAMVGITQLLDGQLHVDSHLLGKDPTESLELIGQAAKAKIIAMAEAENETSETKDKILSDSQLKKTGLPAACVEGQQPLTSRRGLALTSKMSPTKPMEITTKSDEISAGSTSQDTSTTTLASPARLKKRKLTLADETASLAADSPLRHTRVVGTTSAKLTYLIEKVLQHQETEKIIIFYDGDNAAFYIAQCLEMLYINHRIYARSLDNTRRSEYVSLFNEDPDVRVLLIDVACGALGLNLNAASVVLIVNPINRPEIEAQAIKRAHRIGQTREVLVETLVLENTIEHAIFDRARRMSRTQHLEAKELEDDEGITQIIQNAQILPVEQEESEGLSSFALLQTPQQVFGRPNRDRYHRYGLTDAKKITEKRKQKPEASKIEKKITEKRKQKPEASRSEKKVKKKIKINHDDDGITVGSPVVASSSSSNLAILGQKPPSGTDLRDNHSSGTVPSLFNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y746</accession>
    <name>A0A9W8Y746_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Spliceosomal protein DIB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">DIB1</name>
        <name evidence="8" type="ORF">N0V83_006553</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Essential role in pre-mRNA splicing. Also essential for entry into mitosis (G2/M progression) as well as for chromosome segregation during mitosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the DIM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y746"/>
    <dbReference type="OrthoDB" id="147752at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02954">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004123">
        <property type="entry name" value="Dim1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052:SF5">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEN 4A, 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02966">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017199">
        <property type="entry name" value="mRNA_splic_U5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01410">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="39"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR017199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR017199-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4368197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="131" mass="14665" checksum="F4B7E66B9B8D1830" modified="2023-11-08" version="1">MGSLTLQDLPSAWHVDQAILSEDTRIVTLRFGTSTQPSCMTMDNLLSKTAPLVQNFAVIYTVDNTAIKDFNAMYELYDPCTVMFFFRNKHIQVDFGTGNNNKINFEVGEKSELVDIMEAVYRGARKGRAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y779</accession>
    <name>A0A9W8Y779_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005354</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y779"/>
    <dbReference type="OrthoDB" id="5395390at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052109">
        <property type="entry name" value="SRRM_Domain-Containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34755:SF4">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34755">
        <property type="entry name" value="SERINE/ARGININE REPETITIVE MATRIX PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="610"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="717"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="645"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="669"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="746" mass="85325" checksum="A688420AAD9FE211" modified="2023-11-08" version="1">MAVTLKRKRGAVSYKEPSSEEDFSETSGEERTIRRRKRPTPSRRSTRHQQVDDEDESSSQQASPQARPTRNPRNVPRTSRSHGRQKISYKDISSDEEEDDPDADFEVEEVQTPRKTARAAAVRSPRPPRSLDSKRRQRRKLALGAPLKPNNAVVGSEGKAAEIPTDGHKPAWQSLPYHVLLQIFVYAAHPLHDENMKPTTSIPWLVQVARMCSTFTKPALTALYRNPPIFATRQTRKDLVHHLITPPSDAHEDYRVMVKRLELDATQMSALTDQAHSVADLAALVQSLTTLREIDIFDPIDRPPFRDRLRRLRRWVYPTELFEALRQTQLRLRTWRWNRTFCNDGPLWMKTIHGDAAFQTLRELTLTKFHPEPSHEEREKREDLQGQPTTEELLASALVVLPRLRSLSFETCGVVSGRLLPLLPSNLISLNITNCGELLSDALQAFLATHGAQLEELVLHHNQSLSLSFLVDLKRSCPKLEILRMDTIYYSSLAMSSDNEPLYDTLLGVNEAPSWPSSLRVIDMENLRNWNSTSATNFFSSLIEAAEELPWLREITIQAMVDTDWRQRAEFRMKWMAKFEKVFARKWVEPDPHLVSLRAFREWQEHSKNEGKSDSFIEGLPTHTPNGIEVAKTAPDSDSDAPLLRSREQKGDDRWGSKRLRLRSKAATSYDESSGSEIDSGDAQATSDDDDNIFVQGRCHTVMFRVDNFRPREEVYNEGDFLDAEPSGDEEWNGNEDNEDEGGYAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7F8</accession>
    <name>A0A9W8Y7F8_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_005619</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TTC36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7F8"/>
    <dbReference type="OrthoDB" id="539634at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006570">
        <property type="term" value="P:tyrosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038906">
        <property type="entry name" value="TTC36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21405">
        <property type="entry name" value="CDNA SEQUENCE BC021608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21405:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="241" mass="26393" checksum="F5FEB4FCE85AFDB7" modified="2023-11-08" version="1">MAIDIPDTTLSANDKHILSALFDPETLPSSVAKLKDAAAINVSLPPHPSISSSQLSTLEDEQNRIVKQVSSSSDVNIMKEAITQLDEVISTWPNYASAYVNRAMLRRMKMEASLQDTTQNIFSASTSADIEALFTDLARAIHLSLPSSSPTAPVSPYQARILRTAYSHRAYMYLKAVETGTELKGMGKSELEELASKDFAAAARYGDEVAREMSVRTNPYAKMCGAIVRNALREEREGESM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7H3</accession>
    <name>A0A9W8Y7H3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">N0V83_006749</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family. Monothiol subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7H3"/>
    <dbReference type="OrthoDB" id="423313at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005796">
        <property type="term" value="C:Golgi lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000093">
        <property type="entry name" value="Glutaredoxin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694:SF5">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="3">
        <location>
            <begin position="147"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ4368392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="259" mass="28916" checksum="A1C3A43EA064AA74" modified="2023-11-08" version="1">MMPSQRRIRLFGVLIVITVIALVYMSRGGHQTRTSDFYTKTQEALQELRYEEAAKQRDAEDVGSRLKAAEEQAKQAADKKYESKKVAVEGDDQKSVAGRVKIDGDKVPGVAAQGGRSRDQAVVKEDETLEDHEVEMELNAILKKSPIIIFSKSYCPFSKKAKHILLEKYRIVPEPYVVELDINPLGEKLQALLAKSTGRRTVPNILLMGKSIGGGDDMEELDETDTLVDKIKEMGGSRITEVEHRGARAAKPHRRAKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y7S7</accession>
    <name>A0A9W8Y7S7_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Arp2/3 complex subunit, actin nucleation center</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ARP3</name>
        <name evidence="9" type="ORF">N0V83_007259</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the actin family. ARP3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000012">
        <property type="protein sequence ID" value="KAJ4367674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y7S7"/>
    <dbReference type="OrthoDB" id="421448at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10221">
        <property type="entry name" value="ASKHA_NBD_Arp3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000029">
        <property type="entry name" value="Actin-related protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000006">
        <property type="entry name" value="Actin-related protein 3 (ARP3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="23"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4367674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="431" mass="46912" checksum="2A01ADE3DA0504AD" modified="2023-11-08" version="1">MDNGTGYSKLGFAGNDSPSFVFPTAIATKGPTGGSGGSGSGRPQVANKPSFLTGGAGPSGHLSGKRGTEDLDYFIGDEALAAAAGTGYGINYPIRHGQIDNWDLMERFWSNSIFKYLRVEPEDHHFLLTEPPLNPPENREATAEIMFESFNVAGLYIAVQAVLALAASWTSSKVQDRSLTGTVIDSGAGVTHVIPVAEGYVIGSSIKSVPIAGRDITNFVQSLLRERGEPDSSLQTAERIKEEYCYVCPDIVKEFARFDRESDDRFKKHTVNYANGKTTTVDVGYERFLAPEIFFNPEIYSSDFLTPLPTIVDNVIQTSPIDVRRGLYKNIVLSGGSTLYKDFGRRLQRDIRHLVDARIKASEARSGGAKSGGLDVQVISHKRQRHGPWFGGSLLGQTPEFKSYCHTKAEYDEIGPSIVRRFALLGGPGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y891</accession>
    <name>A0A9W8Y891_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">N0V83_006781</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000011">
        <property type="protein sequence ID" value="KAJ4368424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y891"/>
    <dbReference type="OrthoDB" id="2150942at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ4368424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="314" mass="35640" checksum="FF42EF87401BEE41" modified="2023-11-08" version="1">MAPSTRSSLASKINCSKFYFEWKGHPIEDLTTFHNITLEQRPHKPIVYLAGDSSLDNKFWVNRSSDNVPTDVPEIYKKTIKNPVPKPDVALWMNHLLEDRATCINTAVEESMLRDRDDKLLPHDEFIRDNIRAQDVLIVSIGANDIALKPLPCTIRHMLQLAWLTPRSSLENGSAWSLKYFKNLFGTKIQDYITRVTAKTKPRAIIVCMIYYPLEAGLGQASWADVQLKALGYNMYPGQLQTAIRAMYEIATKKIKVEGTEIVPCALHEILDGKDAKDYTDRVEPNEEGGRKMAIRFVELLDQLLEGTHVPDNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y954</accession>
    <name>A0A9W8Y954_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_005221</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000008">
        <property type="protein sequence ID" value="KAJ4370700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y954"/>
    <dbReference type="OrthoDB" id="48317at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08249">
        <property type="entry name" value="enoyl_reductase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047122">
        <property type="entry name" value="Trans-enoyl_RdTase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348">
        <property type="entry name" value="HYPOTHETICAL OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348:SF6">
        <property type="entry name" value="TRANS-ENOYL REDUCTASE APDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="5">
        <location>
            <begin position="12"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4370700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="359" mass="37870" checksum="397C21A3171A5355" modified="2023-11-08" version="1">MNAGAVMKALVGSESGGYRLADDIDIPVPKPGMMLYRVHAVALSPYDAKIVDFSNTPGAVGGCDFAGIVFGVGEGVTRFKEGDRVLAVTFGLNASDKTAGAFAEYALATEDLSCHVPDSLSFEQACSMGLGIATAGLALFQAPGLHLEMPRPKSADENTPSFVLVSGGATGTGTMATQLLKIAGYTPIVTCSPANNAMCEAYGAAACFDYRSSTCGADIREYTANSLVSVFDCVTDPSTMRMCYEAIGSSGGKYVALEAITPLVKYTRRDVRADWLMAPTIMGTPVEIPGTYGRSSTPEHRIFGVELFLLADKLLKEGRIKNHPLEIREGGLASIPTYVNDLRVGNVRAKRQVVPLVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9A9</accession>
    <name>A0A9W8Y9A9_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MFS general substrate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_004323</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000007">
        <property type="protein sequence ID" value="KAJ4371107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9A9"/>
    <dbReference type="OrthoDB" id="268400at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF47">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="200"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="301"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="331"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4371107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="41293" checksum="5DD78776AA6F2EEA" modified="2023-11-08" version="1">MNVKVLLIIVDLTYINQRAIAIALMWSVAGCFGTSGVALVPIISHHGRDWRHFYHIWLIPVAVSLVVTFALLPETYFKRPTVAFDGLIVMQSATEKLTVYQDTEVDSDIYRDLPAYPQRTGVAGFRDRLGLARSPFASWTAAGRCFIQMGYCAVNPLIFWVFVASAFNTASMVFIGATFARVLIAPPYNMAANMVGTVNIASGVGGLFGFVVWSLLICRIVTWLSRRNRGVLEAEHYLVGYIVPILTGATSSLVYGLVVKHHWSANLIFFAYGLNGFSFVTIMIGNTLWVAEAFPRWAAPAIAVVGGGCYILSFAMSFALGPWIAAHGFQWVSIELAILQMVSGLIAMPVAFWGKTARQAIHGRWAEDRSGALRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8Y9T1</accession>
    <name>A0A9W8Y9T1_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_006320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000010">
        <property type="protein sequence ID" value="KAJ4369235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8Y9T1"/>
    <dbReference type="OrthoDB" id="3921198at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4369235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="695" mass="77578" checksum="5FD73E8596BC0441" modified="2023-11-08" version="1">MNERREIAFSPRKIIGKTRQHLRTPATFKDSVDETNPVEIDYKANPWWRSRYLCWEVKGQIAEEYKILGPKIYDELRQSCRESSLEIFIWPYMLGKSTKSARPVLVIASEDEASRADAKATIERSDILKEHQHFKLWPLRYLPTGPVSPVAMEDHATAQPLPSTESFEVYYDPAERLRPIGMPIYIKHSGTTVRRATANAVHNGTNYGYMTAAHAFVEAVPQRAASKDSSSHFDFPFDSDSDSESSNNMDIDASSLYSGTSPETIGRGSSSTASSQRSETSSQTPSSLGDVEELGHIENLNFGDVDYSPVEATTPPKARGQLTSDKPQVDLKSSKIGSRSQPSRSIGSFLSSDKYDVMMEREYSIPIVFGFGPGLKMSVYGLQGMNANALCHKHIIYAWPPDSTKDTHTYVHAVLPAVLTPDVEANLQDAMDTHLLDLVTHHFHAFPLYTSPLKVLREVYVFFRSLETDSAHSLLLTKALKLLVLVHVGGDIVLLNPATDAATDELVRATMPATSLPITPTPCFIRAQFGTAIPALAVRLITEILSSLEQFLPRRSEHDWPAVLATLLVVLMTIESIQYHAAQRPYHEDATAVVARSAIEEGSNRDEEAVQLLLDFYFACFSGCHTRLHPQWAGEPKDSPGPTPELKFIKNIRGAINNMDIRIYLDKKAVERQWDGVDMNFFFDRLVARLFVAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YA25</accession>
    <name>A0A9W8YA25_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="3">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_005427</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="JAPEUY010000009">
        <property type="protein sequence ID" value="KAJ4369665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YA25"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="4">
        <location>
            <begin position="2"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4369665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="380" mass="40573" checksum="3C350E015984BAF8" modified="2023-11-08" version="1">MAFALAWVQKNIASFGGDVTKVTISGESAGGGAVMLLGIAQDGTLGTSLFRNGIAASPYLPPQYDFNANIPTQHYQDFASRAKCTGTGILACLRGKDSTTLQQANAAVTAAGTYGTWAFLPVTDQTFITTTPSKALTAKKVNGLNMLVGNNANEGALFVPANISTEADLKSWLKLEFPVFTDAEVTQVLAAYPVNASTNPNLKFATNGISAPTALDVSQVATGQQQRANDIYAEATFVCPSYWINDAYTSSTRSSYHYQYSIPFASHQDDVPALFGPAQPNQPAAFTSVFRRIWGEFIKYDASTVAQAAMTKWTSGTGAQMGNLNTTGGTPYQTVTQFGATVTQFSNSQPAFSLVNAYTWEGGRGKRCEFWRSIAPKVPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB73</accession>
    <name>A0A9W8YB73_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Extracellular membrane protein CFEM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003158</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB73"/>
    <dbReference type="OrthoDB" id="3695248at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040803202" description="Extracellular membrane protein CFEM domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="191"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4372867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="23145" checksum="2FCAE21167523BB4" modified="2023-11-08" version="1">MLSFIAHRLLFAQVLVLGVAAHRDWWLQGLPKCWQSCLSSTEDGCNSTKCVCKSTEDSASYLPDAVSCAASQCDVDDWALDLVLGPLQLYCSAIDCSIPDQVMASAYAAATSTEEPSQATATAQSSTKTKDSEPQTTTGSDDGQDLTSTVKTTLTQTTTDADGNTIQLVVPIIIGPSGMSTGDVVTSTVDGSASSTEAASSTIASPSPGAEGSSSSEWQWEPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YB74</accession>
    <name>A0A9W8YB74_9PLEO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="6">3.6.4.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">HAS1_4</name>
        <name evidence="6" type="ORF">N0V83_003316</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="6"/>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YB74"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="28"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ4373025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="90" mass="10301" checksum="8A78125CB171D0BE" modified="2023-11-08" version="1">MGDLANRKVFKLTKKQVFNQEFIVDERYNVTKELGQGAYGIVAATNNQTGEGVAIKKVTNVFSKKILAKRALREIKLLQHFRGKSKYTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBC4</accession>
    <name>A0A9W8YBC4_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">N0V83_003557</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBC4"/>
    <dbReference type="OrthoDB" id="5393057at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21383">
        <property type="entry name" value="GAT_GGA_Tom1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAT" evidence="2">
        <location>
            <begin position="208"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="455"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ4373263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="498" mass="54579" checksum="DA3C2FC0B6A68F4D" modified="2023-11-08" version="1">MELKKRLNLGSLMRKRSSQNVNTDPNADTPEANAARGVRLFCESGAANSGEEVLHLPTIVESAVASPTAAAAAAQQIRKFLSKENYNRPHVQYNAVMLIRILADNPGQSFTKNLDKQFADTVKHLLRNGQDPSVSQIVRESLDAMEREKAYDTNLNTLFTMWRKEKGLMANAAKQFGPRKLNAPAWSGQQVQGGFSSGNSRSSSRTLPPPAELAGRIEEARTSAKLLLQLVQSTPPNELLGNDLVKEFAERCTAAQRSVQGYIACDNPAPDDDTMLTLIETNEQLSLAASKHQRAVLQARRLIGASPSPPVPQSNNLPAVIPPQSYEQPNLPPRDVAPSMPPLNVATTEPSQPLPSNPISYKNDELPLPPALQAGPTRQRPNAQEEDPFADHYTSSYTPPPGPPPSQDPRSNGYGGSPDSYHPGYQSTPSYLGRQDSSANNLTMHGAQQSIAEEDEHRRQYPDSQRPRTPELNVPQQQQQQGHDVSPIADRNTVTYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBM6</accession>
    <name>A0A9W8YBM6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">N0V83_003303</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBM6"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF22">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="8">
        <location>
            <begin position="365"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="322"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="335"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ4373012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="759" mass="84004" checksum="B061548999A7895C" modified="2023-11-08" version="1">MRFSNDSGRPVPEIPSLMSSPAVLEMQSTQHQPTVSLDSMAFGHSNEYSLTALSMAAEYQALQGTIPDGGSRPDELSNALPLQPATGPKSVEQHHAMMPIGSPLQTYGSTMEESLDSLTSFLDNEPFNSYHFSSLINTEQPMPFFSPDSFGYGADLAPETDTVAASAPGWRPPHVEEPHSFSRFGSRFPSLQPDDIAQENGPQKRSIADISHEDRQRIAEMLNEFSSVIPCDFKLPTRLALCRYIAAYINGFHEHMPFLHIPSMSVHTCSIELILAMAAVGAQYTFEGEKGVELFHVSRAIATSRIRRRDARLVELQRYSDPDRASSHDLDRTSGIPSPQQSCSHSGPLGLPSDADGAPMGEDLMQTAQALLLLMALCTWAKHKEILREALAIQSIIATLIRDDGLQDEPLRDDISWLDWIRRESIKRTKLIVYGFSNLHCIVYNIPPFMLTSEVKLSLPCSAAQFKAPTEALWQEARKKSVPEVLFQDALKRLFSKNGRDVTEGNSSSGNYALIHALIQQIFFLRQVARCRFDGPSDLSPEDIAALELALRNWQMGWRQNPESSLDPVSPDGPVAFNSTALLRLAYIRLYVDTAGRALETRDPMLIANALRAGPAIKRSPKITRAVLHSAHALSIPIKIGYRLVAKTQSFIWSIQHSLCTLECAYLMSKWLEALSVPNPDPPATDDENKIIAIVKSMLDETEFAVPQEISASSLSYTKHLSAGVLRVWAMIFKGSQTWAIVDVIGTALNLYADMLESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YBY6</accession>
    <name>A0A9W8YBY6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">V-type proton ATPase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">VMA6</name>
        <name evidence="8" type="ORF">N0V83_003277</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments. This subunit is a non-integral membrane component of the membrane pore domain and is required for proper assembly of the V0 sector. Might be involved in the regulated assembly of V1 subunits onto the membrane sector or alternatively may prevent the passage of protons through V0 pores.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the V-ATPase V0D/AC39 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4372986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YBY6"/>
    <dbReference type="OrthoDB" id="10250083at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1690.10:FF:000001">
        <property type="entry name" value="V-type proton ATPase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1690.10:FF:000003">
        <property type="entry name" value="V-type proton ATPase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.50">
        <property type="entry name" value="ATP synthase (C/AC39) subunit, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1690.10">
        <property type="entry name" value="V-type ATP synthase subunit C domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036079">
        <property type="entry name" value="ATPase_csu/dsu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002843">
        <property type="entry name" value="ATPase_V0-cplx_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016727">
        <property type="entry name" value="ATPase_V0-cplx_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035067">
        <property type="entry name" value="V-type_ATPase_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044911">
        <property type="entry name" value="V-type_ATPase_csu/dsu_dom_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11028">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01992">
        <property type="entry name" value="vATP-synt_AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018497">
        <property type="entry name" value="V-ATP_synth_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103486">
        <property type="entry name" value="V-type ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR018497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ4372986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="366" mass="41212" checksum="13E69F47D0DB9B74" modified="2023-11-08" version="1">MEGLYYNVKYGYIEGIVRGYRNALLTSQNYSNLTQCETIDDVKLQLSPAYGDFLSSLPPNPSTSALAAKTTDKLVAEFRYLQANATGSLAKFMEYLTYGYMIDNVALLITGTLHERDTRELLERCHPLGWFETMPVLCVATNIEELYNSVLIETPLAPYFKGSLSHQDLDELNIEIIRNTLYKNYLEDFHQWVNSTPDIAGTPTADVMSEVLEFEADRRSINISLNSFGTELSKADRKKLYPSLGKLYPEGTLMLSRADDVEGVRIAVESVADYKSFFDQTGLSQGSGGGVGNMAGGVGGETRSLEDLFYQKEMEISKIAFTRQFTHAIVYAWVKLREQEIRNITWIAECIAQNQKERIGNYISVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC06</accession>
    <name>A0A9W8YC06_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">P-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">N0V83_003531</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">IMI 356814</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC06"/>
    <dbReference type="OrthoDB" id="2405944at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053226">
        <property type="entry name" value="Pyrrolopyrazine_biosynth_F"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48419">
        <property type="entry name" value="SULFOTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48419:SF1">
        <property type="entry name" value="SULFOTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ4373237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="341" mass="39154" checksum="076956ED12EF0223" modified="2023-11-08" version="1">MSQKPIFVFMTRHKTLQCVHEPFGDAYYFGPERLAERYENDPKARKESGYTESTYRTIFDHIARENSEGKRAFIKDMAQYWIPPNCKPATVAPSLANYRRGVGTDTTALSPVQTRTNSEGPPYPYNTKGEPNNPTVIPADLLATYHFTFLIRHPKHSIPSYYRCTIPPLDKMTGFYNFRPDEAGYEELRQLFDYLRSEGQIGPKFAGQHEANGQAETNGHSGGVEICVIDADDLLDKPSGIIEAYCKTTGIKYEPEMLIWDTEEDQHRAKEAFEKWKGFHEDALDSDRLRARTHKKAPKSDEQLFAEWTEKYGEEGAKVIRDTVAENVDHYEYLKQFAIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YC61</accession>
    <name>A0A9W8YC61_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 10" type="primary">RPS0</name>
        <name evidence="10" type="ORF">N0V83_003345</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">IMI 356814</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with RPS21.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YC61"/>
    <dbReference type="OrthoDB" id="414863at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000010">
        <property type="entry name" value="40S ribosomal protein S0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03015">
        <property type="entry name" value="Ribosomal_S2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032281">
        <property type="entry name" value="Ribosomal_uS2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027498">
        <property type="entry name" value="Ribosomal_uS2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005707">
        <property type="entry name" value="Ribosomal_uS2_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01012">
        <property type="entry name" value="uS2_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11489">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16122">
        <property type="entry name" value="40S_SA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS2 C-terminal" evidence="9">
        <location>
            <begin position="205"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="256"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="263"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="288"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ4373054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="300" mass="32370" checksum="113DB11D376DB440" modified="2023-11-08" version="1">MAPSNLPAIFNPTQQDIEMLLAAQCHLGAKNLQVHMEPYLWKTRPDGVNVINVGKTWEKIVLAARIIVAIDNPADICVISARPYGQRAVLKFAAHTGAVAIAGRFTPGNFTNYITRSFKEPRLIIVTDPRTDAQAIKEASYVNIPVIALCDGDSPTEYVDVAIPTNNKGRHAIGLIWWMLAREVLRLRGTLASREAEWDVMTDLYFYRDPEAEENKDSAGVEEAKVPGADEVGPAAVEAGFTSEWEVSGASAGAFTSQAAQPGASWDADATDWAASGDQQTGNEGWGAETAAAPATTTQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCB6</accession>
    <name>A0A9W8YCB6_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">N0V83_002711</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCB6"/>
    <dbReference type="OrthoDB" id="44015at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16988">
        <property type="entry name" value="ANTH_N_YAP180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000004">
        <property type="entry name" value="ENTH domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000025">
        <property type="entry name" value="ENTH domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-BINDING CLATHRIN ASSEMBLY PROTEIN LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="4">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="277"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="370"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="480"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="370"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="384"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="421"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="487"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="511"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="534"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="552"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="577"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="611"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="626"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ4373972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <sequence length="637" mass="70489" checksum="0115668445D35197" modified="2023-11-08" version="1">MASSFEKSVKGGTKIKLAAPKSKYVEHILVATHAGEAGVAEIFRALTNRLRDSTWTIVFKSLIIVHLMIREGEPEVTLKYLAQNPHRKLAINHFTEVQTQGHNIRTYSEYLLRRAIEYGATKVDYVRGGEGRLKRLTVDKGLLREAESVQDQIRSLLKCQPFDDEPENEITLTAFRLLTMDLLVLFHVMNEGTINILEHYFELSKPDATRALAIYRTFVKQTEAVVSYLSLARSHEHSTRLEIPKIKHAPTSLAASLEEYLNDKDFEINRRQYLAEKEAKKNGGKSTNGTTKLQEVKPAPSQITSSQPSAAPASKPSTSAPLIDLFASLEDNQQTMATQPMMQQYPQQTGFQQQNFQVPQQTANMGFPQQQQSFMPQNGQSTNPFQMQQAPQVQQQPQMQQQPQPQQIQTQFTGAGFGGYTPQQTFSPQNSLAPIPQNGFPDFSQQQQQMQIPTIAEPLQTQQTSTNPFRQSMLPGAAGMAEPKLLNNPTGASSLNRSATNPFAKHNTGFQQQSQSPTSSLPYSASPVQPSPFAPMQQQQQQPLQPLQPAPTGTNPFARQPSPQNAPSPQGGLTVHATGSTNPFRQSAFVNQQTGQGWQTAGGQGTLGGMSLNQVPTTSVFPRPGQQQQQQQQNFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCF3</accession>
    <name>A0A9W8YCF3_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Leucine-rich repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">N0V83_002568</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000004">
        <property type="protein sequence ID" value="KAJ4373829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCF3"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000273">
        <property type="entry name" value="Leucine Rich Repeat domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454:SF69">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 11-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="32"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="280"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="588"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="789"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="293"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="345"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="357"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="374"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="627"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="654"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="712"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="730"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="789"/>
            <end position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ4373829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001140560"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="823" mass="90273" checksum="30A879FFAE1EBF5C" modified="2023-11-08" version="1">MDTEDGQIFIRNLAYFVRTHEKALANALQLQRQAPKHGQSTVASPSSPTGPNSPPSSSSSSVWAAALSLPSLTFTSQSIKPAKLTLTPHHLFYLLSRFEDLSIPVGPMNVRLENIHTDASPTNYVSFLSNAQRNKTKSGDRDSIHSVSSVRSVMSGMSSLWSSLRIGSNSAAKAEKQKAQLQEDLRYLYSAFTKIPCLRLAPDHKARLIAGYEEFPFDTAVPLFAFKNVTALEIYDVDFRQFYGWDRMAENLRSLTVKRAGVDDPADLLINIVLDDMDKRRRRSAKAPPSPSAPWTTASPSAKHAQLARSDSPPSPTAPGKQSTSPRAVVTRERESFVKTHQRQRSTSPTRPASSRHGSTQVYSRNSMSAPNFRRSSGSSGSSVRSTTPRGSSANLLSLGWFPATKWRFLRHLSLADNALTNISAASLAPLANTLQSLDLSANLFAEIPDSLANLTSLRALNLSNCMIEGLHSLGRNPLPAITTLNLRSNRLTTLAGIERLLSLQRVDFRDNKLTDPTEVARLTCVPEICEIYVHRNPFCKTHATYRITIFNLFRKTPGYSEDVVIDSTGPSNGEKKQLVDRAPELLGVPVVKPPPEDDAPPTSHVPPKVVKAIDTPSDVPEALKRTTPHRTKSTRSAHGSQKKKKGPKRRIVELSQSEAAQQSSESSLTGLAYDDMPSGQSPRILKPASTAFVKDTPRFEEPLNGQILGDPTKKTSPDERAVTDDTHTSHQQLPPIDTTTKAKMPESQDADFDISNDLYKKKIEALRQDFGNTWLSALGDENWDTTSVTSFPSDRGYSSPTIRPTLPRTPSQSIVSGGRTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-11-08" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9W8YCP8</accession>
    <name>A0A9W8YCP8_9PLEO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PIN-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">N0V83_003649</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Neocucurbitaria cava</name>
        <dbReference type="NCBI Taxonomy" id="798079"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Dothideomycetes</taxon>
            <taxon>Pleosporomycetidae</taxon>
            <taxon>Pleosporales</taxon>
            <taxon>Pleosporineae</taxon>
            <taxon>Cucurbitariaceae</taxon>
            <taxon>Neocucurbitaria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tapping the CABI collections for fungal endophytes: first genome assemblies for Collariella, Neodidymelliopsis, Ascochyta clinopodiicola, Didymella pomorum, Didymosphaeria variabile, Neocosmospora piperis and Neocucurbitaria cava.</title>
            <authorList>
                <person name="Hill R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">IMI 356814</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAPEUY010000005">
        <property type="protein sequence ID" value="KAJ4373354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9W8YCP8"/>
    <dbReference type="OrthoDB" id="10267235at2759"/>
    <dbReference type="Proteomes" id="UP001140560">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31794">
        <property type="entry name" value="AUXIN EFFLUX TRANSPORTER FAMILY PROTEIN (EUROFUNG)"/>
        <p
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